LOCUS       DAOOVN010000001        66510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_232_length_66510_cov_14.378903, whole genome shotgun sequence.
ACCESSION   DAOOVN010000001 DAOOVN010000000
VERSION     DAOOVN010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 66510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 66510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66510
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_232_length_66510_cov_14.378903"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="PLY83_00005"
     CDS             <1..507
                     /locus_tag="PLY83_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOV28449.1"
                     /translation="RVSGRESLTIAPEAGSDRLRFRCGKAFTNRQILEKLEMASSEGI
                     RKAKLYFMVGLPTETEEDLVAIASLAESIRKELRLGVSLSVNVFVPKPGTSWENQPFV
                     GVADAENRFRILKTSVSERLGKSCDVRFSSLREAELEYRLSWGGPEIVDLMEALVEKR
                     RGGKTDAS"
     gene            641..1255
                     /locus_tag="PLY83_00010"
     CDS             641..1255
                     /locus_tag="PLY83_00010"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOV28450.1"
                     /translation="MLPIVGLVAVGVLVLGIRLFFFPSARVEPTPAGVEQTEATGSAA
                     VKEANPIPASGEVQETVVAVPEGSPLQKIESTPKAKPAQEKPVVKAVPAKASGQAPAE
                     SEAPPSPPKTEAVKPSPKKGTEPAGKENWGVQVGAFKDRGNAENLIKKLRQEGFEAGL
                     IEVASGEATLTKVVVQAGDDRPEAEKLAKTLSDKGYPVLIVKTR"
     gene            1285..2511
                     /locus_tag="PLY83_00015"
     CDS             1285..2511
                     /locus_tag="PLY83_00015"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HOV28451.1"
                     /translation="MSGFVKELISRKEAIEYVSRALDFPWTLNTRAVPLQDAMGKRSG
                     GNLLAPVDYPPFTRSTRDGYALRSADVAGASDSAPVFLRIIGEVPMGEWTEKRIGEGE
                     CVLVHTGGMIPNEADSVVMLEDTVQAGSWLEVRKALQSGENLVSLGEEIKQNDLLLKA
                     GDLVGDRNVGILATLGICTVAIYDLKIGILSTGDEIVPAETPSLPPGKIRDANAWFLE
                     FLLARHGFPAKRLGIVPDIRDRLADSVREAMEENDIVVLSGGSSVSVRDFCSEIMESL
                     PFPGLLVRGINMSPGKPALIAGCRENRRLVLGLPGHPLSCSVVAITLLLPLLYRLAGI
                     ESPLKKMEAVLAGDLFGKAGVEEFIPAILSDGEVLPMPGKSGYVSVLKDANAFVRLAE
                     NEETRRKGEKAEVLQW"
     gene            2505..4433
                     /locus_tag="PLY83_00020"
     CDS             2505..4433
                     /locus_tag="PLY83_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOV28452.1"
                     /translation="MVRACQEHVSLREARKIILEAVKEAGFPRVVRIPPQEGLGFVLA
                     EDVLSRRNVPHYVASAVDGYALLAAETGGATPATVVRLPRDRFQWVNTGSPLPDWCDA
                     VVMVEDTSTAENGDLLLSKALPPGANVRPVGEDVMKGQILARWGERVDPPLIALLLGA
                     GVEEIPVYRRPKCLFLPTGDEICPIQEWAATEEGPRGKVPETNSTLLKGIFQEWGLDL
                     ETHPVLPDIADLIRKKLKAATETYDLVLIGAGTAKGKRDHTAEVISAEGEILFRWVRM
                     KPGRPALFGRVKEALVFGIPGFPMSAAVTAWSLVAPAAFSMSGIPVGEMETEALFRRA
                     LGAFGSLRCEMMLPHSSPPGVEEWLRVKAAGVEGKDLIWILSSGASTMLSMHEADGFA
                     VLPAETVECPKGTGVTVWFRQPVPWSRRALFQGSDDPAFTRIVTPVRKKGADLAIRPV
                     GSLGGLAALARGEGHLAACHLLDGKTGRYNDAFIEEFANGQSWVRVLLYYREQGFLVA
                     KGNPRELSRIEDLVKKGIRIVNRQPGAGTRVLLDWFLQEAGLDRSRIAGYATQAMTHL
                     DAASRVASGVVDAALGIRAAADAFGLDFVTMAWEPFELVIPDKHLEHPGVKAVLESIR
                     EPEWVRQVEALGGYRWPD"
     gene            4421..5413
                     /gene="moaA"
                     /locus_tag="PLY83_00025"
     CDS             4421..5413
                     /gene="moaA"
                     /locus_tag="PLY83_00025"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HOV28453.1"
                     /translation="MAGLMGSFNRRLDYVRISVTDRCNYRCRYCMPEEGVDWLEHDRI
                     LTYEDLLALCRLLHEMGVRKIRVTGGEPTVRKGLTGFLRRLHEEIPALHVALTTNGSL
                     LLSMVEELSSIPLAGLNVSLDSLDPEKFRFLTRCGSLDVVLQGILAFRTRSRVPMKIN
                     TVLMRGFNDGEIPDLLNFAGSCGAVLRLIEFMPLDDSVWSHDRFISADEILESLPDRG
                     KWIPESKARRSPNHGIPEGPARYLVHAETGQRLGLIAAVGHHFCETCNRLRITATGEL
                     RSCLFSPQGVSLREALRRKEMETVRGLIVAEAGKKPKCWKEASASSRHMSQIGG"
     gene            5417..5902
                     /gene="moaC"
                     /locus_tag="PLY83_00030"
     CDS             5417..5902
                     /gene="moaC"
                     /locus_tag="PLY83_00030"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HOV28454.1"
                     /translation="MGELTHLDREGHPVMVDVSAKPETNREAVAEGWVHLPERVFSAI
                     LENQVQKGDVLKIAELAGIQGAKQTASLIPLCHGIRLDQASVRCQLVLERKAVHVECH
                     VKAREVTGVEMEALTGVSVAALTVYDMCKALDKGIEIRDIRLLMKKGGKSGEYRIPEG
                     V"
     gene            5906..6739
                     /locus_tag="PLY83_00035"
     CDS             5906..6739
                     /locus_tag="PLY83_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HOV28455.1"
                     /translation="MRVIGIVEGLNKADGLSMLYLHLGSKGECRVNGKPAEVFASGPG
                     FMERLREGQWQILFSSDCPMKEGLFVAFEKDGPVLLIEEPFETSFKLKPVRGGFLSLS
                     QKATLLRPVNVGVLTVSDKGSRGERKDTAGPALEELVTPIGAVVSARAIVPDDRVLIA
                     ETLRDWTDREKLHLVLVTGGTGLSSRDVTPEALQDVADRIVPGFGEAMRRKTEPLFDR
                     SILSRAIAVTRGSCLLISFPGSERGARQCYEAIGGVLRHALDILNGWEGDCAVHGHTH
                     T"
     gene            6753..7646
                     /gene="rapZ"
                     /locus_tag="PLY83_00040"
     CDS             6753..7646
                     /gene="rapZ"
                     /locus_tag="PLY83_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOV28456.1"
                     /translation="MPETLGSFVDKIVIITGMSGGGKSTALHILEDQGFFAIDNIPPS
                     LLPQLVEVLGKHRAAVLHGVAAVVDIRGDDLLRDLEAVLEGLRRRLASVTLLFMDASD
                     SALIRRFECTRRRHPLGPDLSTLEGIQRERESLAPIREASDVVLDTTGSNLSELRGRV
                     LESLGTPPGRLTVFLTSFGYKHGIPQDCDYLVDVRFLSNPYYEASLRAQSGKDPAVVE
                     YILKASPAAVEFLSKQKELFEFIFPLYEGTGKTQIHIAVGCTGGRHRSVAVVEWFASR
                     LEQTGVSCVVRHRDIDKEQTW"
     gene            7640..8755
                     /gene="yvcK"
                     /locus_tag="PLY83_00045"
     CDS             7640..8755
                     /gene="yvcK"
                     /locus_tag="PLY83_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HOV28457.1"
                     /translation="MVNLEWLTPFGIGILIGGFAVMLWMRRSIRNRRGLAEIMSSAIG
                     YRLAAGPRVVALGGGTGLSTLLKGLKAYTRNITAIVTVTDEGGSSGRLRTEWGVLPPG
                     DVRNCIIALAENDSALNRILSFRFNKGELAGHSLGNLVLLAATELAGDFRLAVEEMNK
                     LLAIRGRVLPVTTEAVTIAGVDAKGNRYRGELDVARHGRELTEVHLEPSDARPLQEVV
                     ESIREAEVIVLGPGSLFTSILPNLLVQGVAEAIRRAEAPRIYVANVMTQPGETDGFSV
                     YDHVRWIKQVLGQYPDIVMVNETEVPKDILQRYRETGSEPVILKEREEVRLAKAKVQV
                     IRGDFLEITPEGLVRHQTQRVSEAIMKVAREAKELGR"
     gene            8752..9675
                     /gene="whiA"
                     /locus_tag="PLY83_00050"
     CDS             8752..9675
                     /gene="whiA"
                     /locus_tag="PLY83_00050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HOV28458.1"
                     /translation="MSAIAEKLWDEWLTAPVNGEREADAEIGALILSMKGKEEKDKTA
                     LSSRRLFLFRRLHRLWNAGTWKEFGELGSFLRFPPDLKGHPALVVPQSMMEKALGILR
                     YLQEGPPGCRWAWLKGVWGGLGSLYLPQTGYYLVFRFEQKKRPIEEKTAALLKLSGIR
                     YTERFRSFHTDLLIRDQAQIVSFLSGLGLYGTGLALEEKSLMRSMRNRANKLVNCDSA
                     NIRKSLDAAERQLELAERVRSSGLFDRLAPSYRELVTTRLENPSASLRELGQMLSRPV
                     TKSTVEYRWSRLGRLLENTVKGDGCHVPWES"
     gene            9656..11620
                     /locus_tag="PLY83_00055"
     CDS             9656..11620
                     /locus_tag="PLY83_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HOV28459.1"
                     /translation="MYLGKADVNTYEKGAGREYLVSNGCGSYCFSTVAGANTRREHGL
                     LVVRPRDCAQHRVLVSKVEETLFTRGKKYQLSTNRYKDVIFPDGFRYIQEFHSNPLPS
                     ILFVVHSLLMRKTVFMPEGRDACIVKYELLASPDRIQLEARPLFAHRLYTDVRPKSTE
                     DSFHADYAANGILVNGRGMSSFLSYSKGEWHPKAFWHDHLLYEKDEIPAPSTEDLWSP
                     GALSIEMEEGETAYLVFSAEPFHPSVEQIEAFEKEAIANLQSRFGKLEPAGQSSAVRD
                     LTRTALHLFASGKDRGMTLFSGYPSLCERARDTFIALPGLTLSMGRYRDAEEILNRWL
                     ELARLNDSVMPSEIDPSTGHALLKAADAGLWFFYALEKLTQRTGSLEFVEKNWEILAE
                     MISRYETAIRVLDLHCEEDLLVLEKPDPERHWMEGTANGSPVVVRSGKLVEFNALWYN
                     ALRAMEQFAEGLGLPEESEKYGKQADRTREAFLQTFWNPEGGFLFDSVDGERKDDAIR
                     PNQILAVSLPSSPLPPEIGRSVVEVCWNELVTTYGLRTLDPHHEKYKGRFEGRLDQKE
                     KARYRGMAWPWLLGQFVTAYMRFNPKRSDIAWCFIRPFTAHLRQGCLGGIAEVFDGSM
                     PYLPHGDVLSAASMGEILRVMEEDLLLQQR"
     gene            11785..12804
                     /gene="gap"
                     /locus_tag="PLY83_00060"
     CDS             11785..12804
                     /gene="gap"
                     /locus_tag="PLY83_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOV28460.1"
                     /translation="MAKIRLGISGFGRIGRLTLRALFQHRHEGQVEVAAINHKPVPIE
                     DLAYLFKYDSVHRTFPGNVQVEGDAIVIDGTPVKVLAQADPEKLDWKSLGVDIVIEAT
                     GKFRDKAGAGLHLKAGAKKVIITAPGKDDDCTIVMGVNEGDYDPENHHVLSCASCTTN
                     CLAPVAKVLQEEFGIEKGLMTTAHAYTSDQSILDHTHKDRRRGRSAAMSIVPTTTGAA
                     KAIGRVIPELKGKMNGIALRVPTPDVSIVDLTAVLKREATPEEINAAMARWAEGPMEG
                     ILSIVDEELVSVDFTGNSASSLFDPSQTMVVGNLAKVLSWYDNEWGYSCRVVDLANLV
                     ARRGL"
     gene            12807..13997
                     /locus_tag="PLY83_00065"
     CDS             12807..13997
                     /locus_tag="PLY83_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOV28461.1"
                     /translation="MKMKGLPETGLEGKRVLVRVDFNVPLRDGAVSDDSRIRAHLDTI
                     RRLREAGARISLISHLGRPKGKASPEFSLRPVAYRLAELTGWDVGFVDECMGPLVAEA
                     VAEARPGSILVLENVRFHAEEEKNDPNFAKALAEPFDAFVMDAFSAAHRAHASTEGIT
                     AFLPSYAGILLCREVEILGQVRDHPKRPFILILGGAKVSDKIGVIGHLLEKVDTLVIG
                     GGMAFTFLKAQGGNVGRSLVEQDRIGFAENLLAEAAARNITVYLPEDIVAGESPDTSG
                     PIEIVPSKAIPEHLMGLDIGPKAAEAFTRAIAGASTVLWNGPMGVFENPHFAEGTRRI
                     AKSLEEATAMGCQTVVGGGDTAAAVGSMGFADKVSHVSTGGGASLEFFEGLPLPGIQP
                     FVLD"
     gene            14015..14806
                     /gene="tpiA"
                     /locus_tag="PLY83_00070"
     CDS             14015..14806
                     /gene="tpiA"
                     /locus_tag="PLY83_00070"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOV28462.1"
                     /translation="MERTPLIAGNWKMHHGISATGKFLSDLEARISRDAGAKKALEGA
                     FVEMAIFPPFTSLWRALEGKRYLPWLKVGAQNVHWASEGAFTGEISADMLKEAGCDYV
                     IIGHSERRQFFGETDEQVRRKIDAAVSAGLRAVLCVGETLQERETGETMAVVEKQLMK
                     ALVGLPPDEVGRSVCIAYEPVWAIGTGRNATPGEAQEVCQAIRRFVAERFGDEAGMAL
                     RVLYGGSVKTENAAGLLSEPDIDGALVGGASLQADSFAQILIAAL"
     gene            complement(14992..15339)
                     /gene="rsfS"
                     /locus_tag="PLY83_00075"
     CDS             complement(14992..15339)
                     /gene="rsfS"
                     /locus_tag="PLY83_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOV28463.1"
                     /translation="MSKEIQTIVKSLSDKHAEKITVMNLGETTTLADTFILATANSDV
                     HMNTLTETTLDTLEELGLPSRLEGASSPRWRLVDAGNLVIHIFSRQGRDYYGIERIWG
                     DAETVEIGEDLPL"
     gene            complement(15387..16643)
                     /locus_tag="PLY83_00080"
     CDS             complement(15387..16643)
                     /locus_tag="PLY83_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOV28464.1"
                     /translation="MKKARLALFFTLCALSIGLGIAWRMHTIVSPGSQEIEESFEHDT
                     LTGNINILAVGIDDVQGSHRSDTIAVVAINIDDRFVKVLSLPRDLRVQIEGHGWQKLN
                     HAYAYGGIDLLRSTISNFLGIPLHYSVIVNYQSFPKLVDLLGGVSVDVPKRLRYTDRA
                     GGLYIDIPAGRQTLNGKTALEFVRFRHDALGDIGRIQRQQLFLKEVLNKLKEPETVAR
                     LPKIVEEATRFFQTEIQPTQAIQMVNYLKDLDRGRFSFETMPGKAAMIDGISYWLGDI
                     ALASQYLTTPPAPVSGDKAESPEGTALVPADAILDRIKGQIAVLNGCGVKGVSQAFSE
                     HLQRLGIDVAFLGNAKHFDYRYTNIAYPENPTAQVLSNAKALGDLCGVPANLVRANRG
                     ATHATIIVGKDYDIILKRLKQYKRNL"
     gene            complement(16650..17312)
                     /gene="nadD"
                     /locus_tag="PLY83_00085"
     CDS             complement(16650..17312)
                     /gene="nadD"
                     /locus_tag="PLY83_00085"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOV28465.1"
                     /translation="MGSLISKGERNLNQTFLARNRVGIMGGTFDPIHYGHLVSAEEAY
                     FALGLSEVIFVPTGNPPHKRTKLVSSPEDRYTMTLLATLDNPHFKVSRLEIDRGGDSH
                     TVDTLREMRHWYAPGSTELFFITGLDGVLDILSWKEPYAIIELCRIVAVSRPGYNSAR
                     LKELPEPIQKAIIPLEIPLLAISSTEIRDRVLEGKSTRYLLPWTVEHYIFKKNLYIRN
                     GR"
     gene            complement(17300..18646)
                     /gene="obgE"
                     /locus_tag="PLY83_00090"
     CDS             complement(17300..18646)
                     /gene="obgE"
                     /locus_tag="PLY83_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOV28466.1"
                     /translation="MKFIDLATITVRSGNGGDGCMSFRREKYIPKGGPDGGNGGRGGN
                     VYLEATRSLQTLADFEFERRFFAESGKPGEGARRNGRSGEDLLIPVPCGTLVFDPSSP
                     EPIADLVEPGDRFLAARGGRGGRGNAVFASSSRRTPRFSEKGMPGEERTLRLELKLIA
                     DVGLVGMPNAGKSSLLAALSKAQPKIASYPFTTLSPNLGVLSVDDDRVILADVPGLIE
                     GSHRNLGLGLAFLRHIERTRFLVHVLDVSDGAGISSVLKQWETVREEFRQYKEALLDT
                     PTLVVGNKIDLLENRSVLEEIRAVFERMGLRFFPLSALTGEGIGNLAKAIVECSRSIP
                     RPTGETRLVALQEEEPGSASASRKRDVVQIVKLRANRGYRVIHPRLEEAVLRYNFEQD
                     EAIIRFSRLLARYRVEELLEEAGAKEGDLVSIGETEFTFKPEKAIEAPASRDEWEA"
     gene            complement(18740..18997)
                     /gene="rpmA"
                     /locus_tag="PLY83_00095"
     CDS             complement(18740..18997)
                     /gene="rpmA"
                     /locus_tag="PLY83_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOV28467.1"
                     /translation="MAHKKGQSSSTNGRDSIGKRLGLKRHDGQVVNAGTILVRQRGTK
                     IHPGLNVGRGKDDTLFALKSGIVRFTTRCGRKEARIETVPA"
     gene            complement(19025..19336)
                     /gene="rplU"
                     /locus_tag="PLY83_00100"
     CDS             complement(19025..19336)
                     /gene="rplU"
                     /locus_tag="PLY83_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOV28468.1"
                     /translation="MYAVIETGGKQYRVSPGEIIRVEKMSAEPGASVSLGKVLFFADE
                     NGVKIGTPFLETVNVEGTVLRHGREPKVFIFHYKRKKNYRKFRGHRQDFTEVRIDAVR
                     G"
     gene            complement(19452..21089)
                     /locus_tag="PLY83_00105"
     CDS             complement(19452..21089)
                     /locus_tag="PLY83_00105"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOV28469.1"
                     /translation="MNMPKGELSEFWVKNIGGIRQARLVLKGNFIAVTGESGAGKSSL
                     VRALELLAGKRGQTASIRFGAEEGEVWGIFREKDSGKEIVAGRILSRGTKNRNFLGEE
                     TVPFARLKDFTESRIGIQSQFSQLDLLDPRRQRDLLDSCGGEESSRLRRKLALDFERA
                     MGLEKELSALQDRRREVERTFLDAEKVIEEVRRLQLKEGNEAGWKEELESVEQFLSSQ
                     KKLLEIANRFVGGASEEGLADEFQRWIREMAGLLDKNQEAALGNSFEILVKEIQKLES
                     FARTLLLDRRLAEAEERRELLENRLGTLRKLKRLVGVESLDELLAFCEKAEKNLDWMR
                     KSSMERESLVQLCRQARKEVAQTANALGQNRRALAAKLEETVTSHLADLAMENFHFGV
                     SLKDLGKIRPSGSDEIEFTLSNGNFSGPIARVASGGELSRILLALQVSLPDDQLPPTL
                     VFDEVEAGLGGRSALLTGFKLRELSGRCQVILVTHEAVIAARADQHFTVRRNGDLTEI
                     SEVTGEERAAEIARMLSGTPDSPEALEHAVALLKETT"
     gene            complement(21086..21952)
                     /locus_tag="PLY83_00110"
     CDS             complement(21086..21952)
                     /locus_tag="PLY83_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOV28470.1"
                     /translation="MKDKIGLLINPQKPKALEIGKRLLLWGAEKGVSFLAPPHEAAVL
                     GIPGAEDEDWKEQVRFAVVLGGDGTFLRAARYVLGNDIPLYGINVGRLGFLASGDPNR
                     AEKDIETILEGRFFTQRRQLLKGTVFRKDAMVHEVYALNDLVVTKGAFARVITLEISV
                     GQRPLSLMTGDGLILSTPTGSTGYALSAGGPIIPPHVHCMLLVPICAHTLYARPMVFG
                     EDDLIRIIPRGDHRDMMLTQDGQLGYELLPGDQICASLAKEKSVSIILLPDRDYYDLL
                     LEKFQWGQNVIE"
     gene            complement(21956..22813)
                     /locus_tag="PLY83_00115"
     CDS             complement(21956..22813)
                     /locus_tag="PLY83_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOV28471.1"
                     /translation="MTRSKERLDKLLVEKGLAPTRAKAQALIMAGLVRVNGSPADKAG
                     QQVPVDLDLTVIGQEEEWVSRGAKKLIKALDVFEINPAGLVCLDVGASTGGFTEVLLS
                     RDASRVYAVDVGYGQLAWKIRTDPRVVVMERTNARYLSRESFPTPIDLIVVDASFISL
                     RLLLPPLLDILKPDGEMAVLVKPQFEAGKERIGKGGVVRSPEIHREVLEEFIDYVRGL
                     PGISLAGLTWSPIKGPSGNIEFLAHLSRVPREGKTLENVKETVAAVVSEAVLALSGKP
                     EREGTEKGD"
     gene            complement(22785..24659)
                     /gene="dxs"
                     /locus_tag="PLY83_00120"
     CDS             complement(22785..24659)
                     /gene="dxs"
                     /locus_tag="PLY83_00120"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOV28472.1"
                     /translation="MGILENLKNYEGFRQLPKEQLPGLCQEVRELMVRVVTENGGHLA
                     SSLGAVELIVSLLRVFDPRKDRILFDVGHQAYAYKILTDRLERFPTLRLEGGISGFPK
                     RRESPFDHFDVGHSSTSISAALGYAKARDLMRQNHEVVAVIGDGALYNGLAMEALNNV
                     KETQSRLTVIFNDNSMSISRPVGGLADYLARLAVNPYYKRFKEYVKDQCRKRQRGESI
                     EDLLSRSKTRMKSLLQPNNIFEGLGISYWGPFDGHNLDELLEIFNLSRFYGQPLLIHL
                     RTKKGKGYPPAEAEPTRYHGIPGKTAQKTKPSGTSWSDVVAHSVRELAETDERIVCLT
                     AAMKDGTRLNGFEERWPNRCFDVGIAEGHLLTYAAGLAAGGMRPFVFIYSTFLQRAMD
                     QLIHDIAMQDLPVVLLVDRAGLVGEDGETHHGLLDVCWGKSIPGMAIMAPRDAEEACW
                     MIRESYRQGHPAMIRFPRGLAPDRLKASGNPDAPQDWGKAEVLIEGRAPWTIMAYGKA
                     VSLALEIHELAAREGLPEPSVLDLRFVRPADWDRIDTILSCNRLACVIEDGFVCGGFG
                     EAIAARANSIPGSPRVLPFGVPDRYIPQATVAAQWRACGLTAKEILEKALDSLEGTAR
                     "
     gene            complement(24682..25584)
                     /locus_tag="PLY83_00125"
     CDS             complement(24682..25584)
                     /locus_tag="PLY83_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOV28473.1"
                     /translation="MTEREEDLDLRLRRCREEVDREIERLLGKDSGIPERLREAMGYS
                     LRAGGKRLRPFLCISAAELSGCPAESVLPMAVALEMFHTASLIHDDLPCMDNDPLRRG
                     KPSNHMVFGEGLATLAGDSLLLWAFETAILNLLPVFPAERILGAISEFAKAIGPSGVC
                     AGQVLDTDPSSRESEVDFVWKIASLKTGALIRASVLAGGILGGLEDVKMEALYNYGTH
                     LGLAFQVVDDILDVTGNSEQIGKTPGKDSVQGKITFVSTYGMEKARRIARDESLKAIE
                     SLSCFGPEADPLRSLARVLLDRSS"
     gene            complement(25581..25811)
                     /gene="xseB"
                     /locus_tag="PLY83_00130"
     CDS             complement(25581..25811)
                     /gene="xseB"
                     /locus_tag="PLY83_00130"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOV28474.1"
                     /translation="MSFSKEIEALQAIVKRLESEPMPLEEAMKLFEEGMAKAEKCKAF
                     LDQARQKVILLSEGDNSGGTAWDPLSGEEAVS"
     gene            25923..26117
                     /gene="rpmB"
                     /locus_tag="PLY83_00135"
     CDS             25923..26117
                     /gene="rpmB"
                     /locus_tag="PLY83_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOV28475.1"
                     /translation="MSKVCQCCGRGPVTGNNVSHSNRHTRRRWQLNLQTVRVDFGEGG
                     RTKLKVCSRCLRSGLVKRAV"
     gene            complement(26159..26881)
                     /locus_tag="PLY83_00140"
     CDS             complement(26159..26881)
                     /locus_tag="PLY83_00140"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOV28476.1"
                     /translation="MKKVEEIRLPQVLLSSPMIPQGEEILLVAGGRKPRVSWLLEAAE
                     NRTVWAIDRGVNACMEAGILPSLILGDADSASPKLWKAATGRGAEVLSLPREKDDTDL
                     QMALKTLAMRLGRIKILVTGCWGGRFDHTWSNVLSVLGTLRERDGYAVLVDHRELLAF
                     IRGGETVTLTQLRRPEAISLLAFSERCTGVSIRGVHWPLEKTELDKGLPYAISNRPKG
                     DEPIVAGLDGGEMGLYVSWWER"
     gene            complement(26889..29162)
                     /locus_tag="PLY83_00145"
     CDS             complement(26889..29162)
                     /locus_tag="PLY83_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOV28477.1"
                     /translation="MARVRKQEPELRERQSSTVRKGSGDSPETAAWIDGAVVFVLFLA
                     VYFLASLYTDLTGAWGQTIRNGFFGIAGGGVVLLLFFVLYCGLFHLLRKPVQDMAKQA
                     GGTIVLYLCLEVVLFIGQGAWPLAAVDWLNPGLLGRAIGEFLLKQTGVLGIVLAGLFG
                     VFLSAALYGLIEPLDVVHWAATIREGLFSGMRRFKDFVSEISLPMAGKLFPRQTEGVS
                     ELSTAPLLSTGGTQLAEPPEISTFSPSAAEEEADDLLVLPVEEALEPADSQEEDTLPE
                     GIDPSAFPPPLELFGNEGITVNPVSDELIRNQGERIISTLEEFGVEAELAETITGPTV
                     IQFRLQLAPGIKVSKVAGLSNDLAVALAVPALRVEAPIPGTTFVGIETPNPRRWIVPL
                     RALLESTPFQRGDFDLPLPLGLTVDGVPLVMGLEDLPHLLVAGTTGSGKSVLVSSCII
                     GLCCARRPDELRLLLIDPKRVEMTLYEKLPHTLTPPVVDSKKAVHALGWAIREMERRY
                     ELFAGARVRHLKAYNRKVLPKDRLPYIVIVVDELADLMMTSPKEAEDYICRLAQMARA
                     TGIHLILATQRPSVNVVTGLIKANIPARVAFTLPSQADSRTIIDLSGAEKLLGKGDML
                     LLTSRYPKPLRVQGPWIDESRISLFIQHVLRVFGEPQHIDIDSDENGAWGEIAANLED
                     PLLEEAVSIILQTGIASASRLQRQLKIGFTRAARLIDTMEQLGMVGPQEASRPREILV
                     DEDKAEEILRQAFSGGS"
     gene            complement(29149..30549)
                     /locus_tag="PLY83_00150"
     CDS             complement(29149..30549)
                     /locus_tag="PLY83_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOV28478.1"
                     /translation="MNVEPLAEGWNFLGEQLAALSGKTVDYVDLFVQSASGHSVTFEK
                     GRVEEVSSGASGGVGARVLLDEHSVFSHSPDSSLKGAWLCMGETLARAGLPSLSTLSF
                     TPAGYTDFEPVRLKAPETDPLGEVDRSIRNASPHVRQVTLRAKVSGRRTLIVRGDGKI
                     VTDLRFHSLFAVQVTVERKGTIQTGYEVRAFQTGPGNFWDDLFPLEVGQKALERALLM
                     LEASQCPAGRMPVVLSGQAGGTMIHEACGHGLEADIVQKDYSVYRERLGEQVASPLVT
                     LVDDATIPRAYGSYESDDEGTPAQRTILIDRGVLKAYLTDVQSAKKGGLPLTGNGRRG
                     SYRNLPLPRMSNTFLLPGEDPLESLLDKAGKGLLVTKMGGGQVNPTSGDFVFQVTEAF
                     LIENGRIGKPVRGATLIGNGPETLKQIEAIGGDLHFEPGLCGKDGQSVPVTDGQPSLL
                     IRDLLVGGCEIGDGEG"
     gene            complement(30539..31681)
                     /locus_tag="PLY83_00155"
     CDS             complement(30539..31681)
                     /locus_tag="PLY83_00155"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOV28479.1"
                     /translation="MTFFQGKEPPAVCPGCGAFLQSDDPEAPGFVPEGLKPRQGLVCR
                     RCFRLVHYRQATKAPLSDEQVFGILRREMENADGGIVFLDALQLAPNPRLEKRLKEWG
                     KPFLKVINKYDMLASWFPEGSPNGRLEAILGSSKDEFLTMNALDHAAVKKALAWLERR
                     FPSGARILLMGPVNSGKTTFLRSVCAEARFASSSPMPGTTLGPIEVSNSRLGMTFVDI
                     PGFRSDDPWIPVLCPDCLFRLQPEKQIVRSVYSLSFGDALLFGGLVEVKVLNYPKGNH
                     LKIAAFTPESLKPHRTKLQRTAELVEKHAGGLLKIPCRGCRTILDGIPRTEAEVQISK
                     GQDLVLPGCGWITLWRGEGLFSVAAPHFFKFRTRAALIRGDEIDER"
     gene            complement(31678..32727)
                     /gene="mltG"
                     /locus_tag="PLY83_00160"
     CDS             complement(31678..32727)
                     /gene="mltG"
                     /locus_tag="PLY83_00160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOV28480.1"
                     /translation="MIRSGGVGIALLTLLLFGTIVSKHGAWWEKNVPLGKGAPVEALV
                     EPGQNALAVARSLVEQGVTSGDARVLSRWFSLLGMDKRLQPGLYRIRPGTPWEIARQM
                     QHQEPDSAGVRLIPGTSLGELAAGLSSWGGEAVLLRELADPSNFPPAMRSYLPEKPED
                     RLAFLLPDTYLVTPSQTALKDLVRLASRKWLEKVGSRVPERVSPQWIRERAILASINE
                     REAKDNSERARVAGVFENRLRLGMPLQSCATVIFAWKQRGRPLNRLSYKDLEVDSPYN
                     TYRNKGLPPGPICIPSEPAWRAALFPEKHEYLYFFLSPSGSHLFSRTYEEHLAAQGTA
                     SRDENQDGKGIEGKP"
     gene            complement(32761..33387)
                     /locus_tag="PLY83_00165"
     CDS             complement(32761..33387)
                     /locus_tag="PLY83_00165"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HOV28481.1"
                     /translation="MLSDLSLRDFVKELGSASPAPGGGSAAALAGALASSLCSMVAAL
                     TAGKERFRDVWPEMAEAGEKSRQLTEKLIELMDQDAEAYNHVMEAIRMPKSTEEEKSL
                     REQAIQEALKSACRVPLETVRTVAETASIMESLLFKGNPNAVTDVGSAGRLAWAAAHA
                     AAYNVEVNLRGISDPSFVEETRRQLRFELSKVEASAIVAEAEMQKRFS"
     gene            complement(33406..34650)
                     /gene="hutI"
                     /locus_tag="PLY83_00170"
     CDS             complement(33406..34650)
                     /gene="hutI"
                     /locus_tag="PLY83_00170"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HOV28482.1"
                     /translation="MTVKLFVNACCFTPIDPGHPLSGNDQGMIREIPKGAILTQNGRI
                     LQIGPEPEVLEAVRHLPPDQEIDCGGHCVIPGFVDPHTHLCFHGLRESEFSMRLEGIP
                     YLEILRRGGGILSSVKRVRAAEEEELFASTLQLAQSALAHGTTTIEIKSGYGLETQAE
                     LKMLRVIDRVGRQSPLDVIPTFMGAHAIPEEYAGDSEAFVEMIIHEMLPEVASQGIAR
                     FCDVFCEEGVFSIAQSRRILEAARALGFGIKIHADEVHDLGGAGLAASVGALSAEHLL
                     AASDEGLKAMAAAGTIAVLLPATAYSLGKPFARGREMIRLGVPVALATDCNPGSCFCE
                     SVPFVFGLAVLGMRLSPEEALTATTLNAAYALGREKSCGSLEPGKKADFLILEGQTPA
                     ILAYHAGGSPVKAVYKGAERVI"
     gene            complement(34647..35570)
                     /gene="ftcD"
                     /locus_tag="PLY83_00175"
     CDS             complement(34647..35570)
                     /gene="ftcD"
                     /locus_tag="PLY83_00175"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOV28483.1"
                     /translation="MSRKLLECVPNFSEGRRPEVIEEIVTPFRKRAGCTLFDYRADPD
                     HNRLVVSLVGEPEALAESLLLAAEVAVRHIDMNHHQGAHPRIGAIDVIPFTPVQGMTM
                     EECVAFSHEFGKRLYEKTRIPVYFYEESALRPERTRLEVIRKGQYEILKNEVEKPERS
                     PDIGEPRLHPTAGATVIGARKFLVAFNVNLRTTDLAVAKRIAAAVRASSGGLCAVKGI
                     GVALEDKGMVQVSMNIVDYEKNALYRVLEMIRFEARRWGVEIAETEIYGMIPARAILE
                     SAAYYLQVADFDPMQVLELKLLETMGSEEAR"
     gene            complement(35577..37622)
                     /locus_tag="PLY83_00180"
     CDS             complement(35577..37622)
                     /locus_tag="PLY83_00180"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOV28484.1"
                     /translation="MSINSEISKAMTIRLDGPFPPDPVFEPGIRRAPNRGYSLNRRET
                     ELALKNALRYVPEEWHERLAPEFMQELLTRGRIYGYRFRPQGRIWGRPIDEYEGKTVE
                     GRAFQVMIDNNLDFDVALYPYELVTYGETGQVCQNWMQYRLIMKYLQVMTDEQTLVVA
                     SGHPLGLFRSRPDSPRVIITNGLMVGMFDNQEEWHRAMQLGVSNYGQMTAGGWMYIGP
                     QGIVHGTFNTILNAGRLKLGLGPNEDLSGRLFISSGLGGMSGAQPKAVEIARGVGIFA
                     EVDASRIETRHSQGWVSRVTADLDEAFRWAEKAMAEKEPLSIAYHGNIVDLLEYAVNQ
                     GRKVDLLSDQTSCHAPYEGGYCPQGLTFEERTEMLAKDREEYRRRVDESLRKHFSLIK
                     TLVERGTYFFDYGNSFMKAVFDAGVKEIAKNGRDELDGFIFPSYVEDIMGPMLFDYGY
                     GPFRWVCLSGRHEDLVKTDRAAMECIDPNRRGQDRDNWIWIRDAEKNALVVGTQARIL
                     YQDAEGRMRIARRFNEMVRRGEIGPVMMGRDHHDVSGTDSPFRETANIKDGSAIMADM
                     ATHCFAGNAARGMSLVALHNGGGVGIGKAINGGFGLVLDGSEKADRAIELSLAWDVMG
                     GVARRAWARNENALATSMEYNRQHPGTDHITLPFIADEDLVRETVKKALDGTLGKDA"
     gene            complement(37719..38873)
                     /locus_tag="PLY83_00185"
     CDS             complement(37719..38873)
                     /locus_tag="PLY83_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOV28485.1"
                     /translation="MRGFRVFAAVLVILCLLIGVASAAETIKIGMLAPLTGFAAADGA
                     SALNSVKIAVDQVNGQGGVLGKKIQLVVYDDRADAKEAVALAYKLIEQDKISAFVAGS
                     YSLPTRAVAPIFQEEKIPLVAAYAIHPDVTKAGEYCFRNGFLGTVEGKAAAYTAHKLL
                     NAKTVALLTSDNDFGRTLVEGFKMYQAKYGRDMKIVYEQAYPFSEKDFKPYLSKIKEA
                     NPDLIFASGYYFQTGPLLKQARELGIKSKILGEEGADSPKLAEIAGEAAEGFYIVTNF
                     DRDDPRPVVQNFLKEYRKRYKIEPDMVGASAYDAFMIIVDAMRKTKTYSNPTAIRMAI
                     SKTTNFDGLTGIIKGFTHIGEVVKPVQVQVVKNGLFRRFGVVDDPELIRP"
     gene            complement(38925..39635)
                     /locus_tag="PLY83_00190"
     CDS             complement(38925..39635)
                     /locus_tag="PLY83_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV28486.1"
                     /translation="MLLEVRDLRIAYGDIEAVHGVSFSVEEGELVSIIGANGAGKSSI
                     LNAIMGLVPARSGSVRFKAAEISRMAAHERARAGIRLVPERARVFPRLTVRENLRVGL
                     YGIHDRAREEKQLEWLYSLFPILAERSGQNAATLSGGEQQQLAIARALISDPRLLLVD
                     EVSMGLMPKLVDEVFKVLQELNRQKGLTILLVEQNALASLEISRRSYVLETGRIQFEG
                     ESAVLKDDPRIREAYLGL"
     gene            complement(39625..40392)
                     /locus_tag="PLY83_00195"
     CDS             complement(39625..40392)
                     /locus_tag="PLY83_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV28487.1"
                     /translation="MDEPLLSLREISMHFGGLKALDRVSFDLHEGEILGLIGPNGAGK
                     TTCFNTVSGVYRPTSGEIRFRGKRTDGLPPHRMAALGIGRTFQIVRPFPNLTVLENVI
                     VASGLTCYGRLIPSCRSWKGRKTREEASEILARVGLESQAGQKAGLLPLGNLRRLELA
                     RALALKPHLLLLDESFSGLRHEEIAQQEELVRKTRESGISILLIEHNMRVAMGLCDRL
                     VVLDHGRKLAEGKPEAIRNNPEVIRAYLGRGGSANAS"
     gene            complement(40392..41273)
                     /locus_tag="PLY83_00200"
     CDS             complement(40392..41273)
                     /locus_tag="PLY83_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOV28488.1"
                     /translation="MSEYTITIGTLIAIQAIVACGLNVIVGYAGQISLGHAAFFGIGA
                     YASALLTTAGGLSFWTALPVVLGITGLVGLFLGLPSLRLREDFLAITTIGINFIVVSI
                     FQYVPFFGGALGIGGIPRIVLFGERIRGVSFFLLSAGFLAATLAICWLFTRSWSGLAC
                     FALREDETAASSMGVSPVRFKLTAFILGTAMAGLGGALYAHFMRFISATDFGFPMSVM
                     ILSMVVLGGMGTLWGPVLGALVLGILPEVFRPLTDYRMFLYAALLLLMIRFQPGGLLG
                     ENSLARRTFSRLKGGKS"
     gene            complement(41290..42156)
                     /locus_tag="PLY83_00205"
     CDS             complement(41290..42156)
                     /locus_tag="PLY83_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOV28489.1"
                     /translation="MQLFLEQMLNGIAIGAIYAMITLGLALVYGILRILHVAHAAVYT
                     VGAYVGLAVFAATQNILLSAAGAMVSCAGLGFLIERFVYYPLLKYPPYVPLIGSIACL
                     LAIEEICRLAAGPYILSFPARLPFPSFTVGGIVISSTLTAVYCISAGVLALLWFITTR
                     TELGLAMRATSQDMAIASAMGINSHRIISLTFMIGSAVAAIAGILVGIYYNQVYPSMG
                     AVPAYKTLALIVVGGLGSVPGAVLASILLGVGETLLIGYAGIPLPRDALAFIAMIAVL
                     LWRPQGLLGSRG"
     gene            complement(42179..43711)
                     /gene="hutH"
                     /locus_tag="PLY83_00210"
     CDS             complement(42179..43711)
                     /gene="hutH"
                     /locus_tag="PLY83_00210"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HOV28490.1"
                     /translation="MDGKVIALNGHSLSLEDVVKVAREGFGVSLDPAAVTFVERGGRI
                     VREWARDGKVIYGITTGFGDLASVSIPPDQSELLQKNLLLSHACGVGEPLPEDAVRAI
                     MLLRINTLTRGFSGISLETLTQLVNYLNLGIHPVIPSQGSVGASGDLCPLSHLAITLL
                     GEGEVFYGGRRMPTARALQETGLKPVSLKPKEGLALNNGTAFMAGLAALAVYDASLLS
                     KVADIAAALSLEALHGVPYAFDPRTHELRPHTGQKLVAENIRTLIQDSRIIERFKGNR
                     VQDAYSLRCVPQVHGASRDALEFVRGKIDLEINSVTDNPIIFPTDGIAISGGNFHGQP
                     IALAMDFFGIAVAELADISERRVARLVDHKLSGLPPFLVPESGLNSGFMIPQYTCAAL
                     VSENKVLAHPASVDSITTSACQEDHVSMGAHAARKARTILENTFNVLAIEILTAAQGI
                     DFSLPLKPGKGTEAAYAAVRETVPFYEKDAFMEPLIREVHRITRSGKIVAEVEAAVGR
                     LN"
     gene            complement(43756..44772)
                     /gene="amrS"
                     /locus_tag="PLY83_00215"
     CDS             complement(43756..44772)
                     /gene="amrS"
                     /locus_tag="PLY83_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOV28491.1"
                     /translation="MNGSEAGAARALWWNRENGSVRCTLCPHRCLIQPGQRGRCGVRQ
                     ASADGQTLEALNARLAAAIHLDPIEKKPLFHWQPGSRILSVGTVGCNLACPFCQNWEL
                     ARWDPSVGLFRITPQGLLKAARDSGSRSVAFTYNEPLVWYEFLTEAAPLLQREGFDVV
                     LVTNGTINPGPLEAILPFLSAANVDLKAFSETAYRKMGGYLQPVLETICTLKKSNVHV
                     ELTHLLVPGINLERASFEDMVEWIAGLDETIPLHLSRYFPRWKWTEPATPLPVMEEYR
                     QIASGRLFYVYPGNLPGLGITRCRKCGRDIVVRDGYNVVQLELDPLGKCRFCGQENGF
                     VVHR"
     gene            complement(44769..45347)
                     /gene="amrA"
                     /locus_tag="PLY83_00220"
     CDS             complement(44769..45347)
                     /gene="amrA"
                     /locus_tag="PLY83_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOV28492.1"
                     /translation="MGYGIAFWEKAERKGLPHLAREAVLFYLKTGKRLPVEQARQILP
                     DPKIWTERKACFVSLKERRNGNLRGCIGTIEPTCPSLGEEIIRNAIAGATQDPRFEPV
                     SLAELQDLVFSVDVLGRPERVSGPESLDPKRFGVIVEKGNRRGLLLPDLEGVDTVDEQ
                     LFIASRKAGLQGPEGADLWRFTVERFREQEEQ"
     assembly_gap    45399..45498
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<45499..45996)
                     /locus_tag="PLY83_00225"
     CDS             complement(<45499..45996)
                     /locus_tag="PLY83_00225"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV28493.1"
                     /translation="MIEERRGGETMWLWGCLAPHPPIIVPAVGRGREREASKTLQGMK
                     ILAEKVKCKKPDLLFVLSPHAPFGNGLMFVDADTFEGGLQQFGVPGSFFTAPGNPEAV
                     QKLSAFLGGSIRLRTWRTSKFALDHASVVPLTWFAEAWGQLPPLVLANPIGLDLDAAY
                     SLGKKL"
     gene            complement(46050..47129)
                     /gene="queA"
                     /locus_tag="PLY83_00230"
     CDS             complement(46050..47129)
                     /gene="queA"
                     /locus_tag="PLY83_00230"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOV28494.1"
                     /translation="MNGKDLEGGMPKDTLPDLLKIDAYDYDLPPEQIAQVPATPRDSS
                     RLLVLKRSTGLLEHRVFREIPEFLRPDDLLVLNDARVIPARLLGRKMGGSARCEIFLL
                     RPTHPDWREWEALVRPGRRLQPGTTVLLAEGSTVRIEERLPEGLRRVQFPEALDVHDL
                     IAKAGQVPLPPYIENDTIDPERYQTVYASREGAVAAPTAGLHFTESLLENIRSRGIEI
                     RRLTLHVGIGTFRPVKESDIRNHPMHEEFFEIPEETAQSVNEALRRGRRIVAVGTTVV
                     RALEASSQTGTCLPGINRTRLFIYPGYRFRAIGALITNFHLPRSSLLMLVSAFAGHEP
                     MMKAYQTAVREKYRFFSFGDAMFIE"
     gene            complement(47148..48530)
                     /locus_tag="PLY83_00235"
     CDS             complement(47148..48530)
                     /locus_tag="PLY83_00235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOV28495.1"
                     /translation="MKKPIHFFSVLLPVAIALFFLLPSSHLEASSRQVRVGLETGVFS
                     GRIDSNSAIRVLVASGGSFSAGATASVESVPGGILVNGKQVSLPAEFESSQGFLYWNG
                     KPYRGRLRLVSRQNGFSVVNVLDVESYLRGVLKIEVNPAWPMEALKAQAVIARTYALR
                     QAGRHGAEGFDLCATTHCQVYRGMAAEDPKLDRAIAETRGIVVIFGSELAQTYYFADG
                     AGWTADVSSVWGGKTPYLTCRPEPVPYETPHSRWRASLTQEALQKIMNTLRQPVGSIL
                     EIRALQRDAGNRVTLLEVRGTSGNATIKGHSFRMAAGSNLIRSTLFEVSPAYGSTFFN
                     PPEKPVPGPAAPPGAPGEDPLVVLTRQGAFTSDELMDMLLHPEKKTDYLKKALSKGET
                     KASVPLPAPAPSAPFPGTVPPAFEFQGKGWGHGVGLSQWGAKALAENGWKFEAILGHY
                     FPGTTLQAIL"
     gene            complement(48527..48757)
                     /locus_tag="PLY83_00240"
     CDS             complement(48527..48757)
                     /locus_tag="PLY83_00240"
                     /inference="COORDINATES: protein motif:HMM:NF022596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 family protein"
                     /protein_id="HOV28496.1"
                     /translation="MNLYTFGKTLVQIGLLLVLLGGLLLLAGKAGVPFGRLPGDLSFR
                     GKHFILFAPIASMLLLSLVLTILLNLFLRWFK"
     gene            complement(48754..49797)
                     /gene="ruvB"
                     /locus_tag="PLY83_00245"
     CDS             complement(48754..49797)
                     /gene="ruvB"
                     /locus_tag="PLY83_00245"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOV28497.1"
                     /translation="MNQETNRMVEPFRGVGETDLLSLRPQSLEEFVGQKSLREKLRIY
                     IDAARMRKEPLDHLLFFGPPGLGKTTLAGIIANEMSGNLKVTTGPALERAGDLAALLS
                     NIQAGDVLFIDEIHRLSPNVEEILYPSMEDFSLDIIVGKGPFARSIRLKLPAFTLIGA
                     TTRLGLLTSPLRARFGIVEQLDLYTAEELSEIVMRGAALLKIDIDPSAAGVIAQRSRG
                     TPRVAIRLLKRVRDVASVTNHARIDASLAKEALEMLSIDHSGLDEGDRKLLRALIELF
                     SGGPVGLATLAAALNEEQQTVEDIYEPFLIRKGLIERTPRGRRATPAAFEYLGIKPPA
                     GYARQMPLLEGAE"
     gene            complement(49794..50408)
                     /gene="ruvA"
                     /locus_tag="PLY83_00250"
     CDS             complement(49794..50408)
                     /gene="ruvA"
                     /locus_tag="PLY83_00250"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOV28498.1"
                     /translation="MLRSLFGQVIQVEDETVVLDVNGLGIEVHCSRRALESCVEGEMT
                     RIQTFLLVNESGISLFGFANDEERRLFLELLAVKGIGGRMAMSLLRAEPPGRLIRAIL
                     SSDLETLTRVPGVGKKTAERLCFELRERLAKSFPSFTRDGAEPASSPAGSEVLEALVG
                     LGFSRHEAAAAVRTALSQADSGAREEEILESALRLLNRIRGEYR"
     gene            complement(50402..50932)
                     /gene="ruvC"
                     /locus_tag="PLY83_00255"
     CDS             complement(50402..50932)
                     /gene="ruvC"
                     /locus_tag="PLY83_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOV28499.1"
                     /translation="MADRRSIRCLGIDPGIGRMGFGLVAQAGSTLTAVEYGCIETPPG
                     DLLQKRLLCLFSSLEEIFHRVSPDFLAVERLYFGRNTTTAEAVWQARGVVLLLAAQRG
                     LEVFQPKPAEVKMAVAGNGNATKAQVQHMVCEILRLPAPPRPDDVSDALAIALTGLAI
                     RSVQSRLNPAGGPAPC"
     gene            complement(50925..51680)
                     /locus_tag="PLY83_00260"
     CDS             complement(50925..51680)
                     /locus_tag="PLY83_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOV28500.1"
                     /translation="MSGHSKWANIKHRKGAQDAKKGAIFQKLAKFIIVAAREGGGDPN
                     ANFRLKTAIEKAREYNMPMENIERAIKRGTGEIEGATYEEITYEGFGPAGVAILVETL
                     TDNRNRTSAEMRSLFSRNGGNMGEAGSVSWMFERRGVISVTGKGLDEEKLMEASIEGG
                     ADDLVPDGEDGYTVYCDPSVLSQVKEALAAKGYAVANAEVSMVPKNSVEITKKEDAAK
                     VLKLMEILEDHDDVQNVYGNFDIPDEVMEAIAG"
     gene            complement(51698..52453)
                     /gene="nadE"
                     /locus_tag="PLY83_00265"
     CDS             complement(51698..52453)
                     /gene="nadE"
                     /locus_tag="PLY83_00265"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOV28501.1"
                     /translation="MPTFYRDPSILLSSIENWLAEQLRSAGGKGGIVGLSGGIDSAVV
                     SAILKRVCGNRMLAVIMPCHSHPEDRLHAEMVAVHLQIPTMTVDLTQTYDALISALGQ
                     DAGEENRLARANLKPRLRMTTLYFLAQARNLLVCGTGNRVELTVGYFTKYGDSGVDLL
                     PLGDLLKGEVRQLASLLEIPAPIIEKPPSAGLWQGQTDEGEMGVTYEELDRFLALGEG
                     SDRVRSFVEQSRARSEHKRQTPPICRISETKQA"
     gene            complement(52531..53304)
                     /locus_tag="PLY83_00270"
     CDS             complement(52531..53304)
                     /locus_tag="PLY83_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOV28502.1"
                     /translation="MPPVRVAIIQTGSGPDSPLERVFQVDSFLRELPADVSLVILPEL
                     WSVPHVDEALPENARFSPSLLERLSDEASRRFCAIHCGSLPWVEHGFLFNRSFFLNEE
                     GRVIAHYDQTHLLPSVEEDSFFRPGKATSPFFWKGSVLGIAIGRDLLFPEFIRTLALA
                     GSRILLLCASWPENQMAQWRALLQARSIENQIFTVACNRCGHSMGIAPDGTLLAEAGK
                     GPETLVFEMDPAATERVRKKLPLLEGRRRDLYSLLSSMS"
     gene            53345..53755
                     /gene="nikR"
                     /locus_tag="PLY83_00275"
     CDS             53345..53755
                     /gene="nikR"
                     /locus_tag="PLY83_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HOV28503.1"
                     /translation="MTEKLVRFSVSMPEALVSEFDRWIEKKKLKSRSDALRQIIRDYI
                     GQSLWEVQEGEAYGTFTFSYDHHSRDASSEITRLQHDFMESIVCTTHVHIDHDTCMEV
                     VVLRGSTQEIVRFVEAASSLGCIRVGVPGLLPPI"
     gene            complement(53821..54765)
                     /gene="argF"
                     /locus_tag="PLY83_00280"
     CDS             complement(53821..54765)
                     /gene="argF"
                     /locus_tag="PLY83_00280"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOV28504.1"
                     /translation="MPVNLKGRSFLTLKDFTPEEIEYLLKLSADLKTKKRMGIRGNLL
                     AGKNIALIFEKPSTRTRCAFTVACIDEGAHPEYLGKNDIQLGHKEDVKDTARVLGRMF
                     DGIEFRGFKQSVVEELARYSGVPVWNGLTDTYHPTQILADFLTIQENFGHLKGIRLVY
                     CGDGRNNMANSLMIGSAKMGLHFVIAAPKSLWPESDLVETCQAIAKETGATIQFMEDA
                     MEAVKGADAIYTDVWASMGEEDKMAERVALLSPYQVNSKLMAATGKENTIFLHCLPAV
                     KGKEVTEEVFESKNSKVFDEAENRLHTIKAVMVATLAN"
     gene            complement(54825..56066)
                     /locus_tag="PLY83_00285"
     CDS             complement(54825..56066)
                     /locus_tag="PLY83_00285"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HOV28505.1"
                     /translation="MDKGTSGFNVSSEVGRLKRVMLHRPGKELERLTIDNKDELLFDD
                     ILWVEEAQKEHDAYADLLRDNGVEVVYFRNCLAHVLREEEIRSMLLDEVLAWEALDYP
                     LLCGLKTEMMSVPAPELAEILTGGLTKAEARELLGNCQSLVLNTLADHELLIRPLPNL
                     YFQRDPYSFVKNGVIISVMSFSARQREPIYARYIFKYHPYFKDVKIFFGEEKLDGVPY
                     CIEGGDFHVLSEDTVAIGISQRTTAGSVQIVGRRLAKEAGIRTVLAVDIPKHRAYMHL
                     DTVFTMVDWDAFTIFPGVANRIRVWELSYDEGGNLVRIDESLNLVEVLKKSLHLDQVR
                     LIETGGGDPVLAARDQWNDGTNTFSIAPGVVVTYRRNVVSNTVLRENGIKVYEIRGAE
                     LGRGRGGPRCMSMPLEREPKR"
     gene            complement(56155..56997)
                     /gene="speB"
                     /locus_tag="PLY83_00290"
     CDS             complement(56155..56997)
                     /gene="speB"
                     /locus_tag="PLY83_00290"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HOV28506.1"
                     /translation="MTRFIGSSRNLQASKWVLLGIPMDFTVSRKSGSRFGPESIRAES
                     YNLETYSPALDRDLSEVTFFDLEDLHFPYGDVHQALDTTERVAQRLIARGKRIGSLGG
                     EHLISLPLIRAAASREPELRVVHFDAHADLRTSLRGGTLSHSSVMRHVAQSCLGEPDR
                     LYQFGIRSGCKEEMAWGKAHTRLHLDDVLEPLSRRVDELKPFPVYLSIDIDVVDPAAA
                     PGTGTPEAGGIPSRELLEAIRTMKGLNLVGFDLVEVAPDLDVNNVTSALAAKCVREIL
                     LQWG"
     gene            complement(56994..57857)
                     /gene="speE"
                     /locus_tag="PLY83_00295"
     CDS             complement(56994..57857)
                     /gene="speE"
                     /locus_tag="PLY83_00295"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HOV28507.1"
                     /translation="MEPMARRETDLWFTEYQTPDMRLGLRISEILLNIQTPYQHLLVV
                     KTEQYGNLMALDGAIMITEKDEFTYHEMMSHVALCSHPSPRRVLVVGGGDGGIVRELV
                     RYDILEKVTLVDIDEKVIEASRRFFPSVSCALDHPKVEIRPMDALVYIKDHQDEFDVI
                     IVDSTDPVDFAAGLFEEAFFRDVHRALKKDGMVVSQTESPMGHPDILRTAYLEMKKVF
                     PIVELGWGIMPTYPTGSWTYTFASKVHDPRKIFREPPEGTRYYSSKIHEACFTLPPFL
                     EKILEEGPNQA"
     gene            complement(57864..58343)
                     /locus_tag="PLY83_00300"
     CDS             complement(57864..58343)
                     /locus_tag="PLY83_00300"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HOV28508.1"
                     /translation="MIETPDLYTLVVGKGEGEKKLTAFDAALLEAGVGNMNLLRVSSV
                     LPPRCEFAQKMEMPYGSLLPIAYGSISSDVPGERIAAAIGVGVPEDPGSFGMIMEYSG
                     FCSADEAARKVEEMVREAFEMRALALREVKVKAVDHVVEQCGSVFAGCPLFYRKKGE"
     gene            complement(58508..59563)
                     /gene="ispG"
                     /locus_tag="PLY83_00305"
                     /gene_synonym="gcpE"
     CDS             complement(58508..59563)
                     /gene="ispG"
                     /locus_tag="PLY83_00305"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOV28509.1"
                     /translation="MSVKSVSVGCLGIGGGNPVRVESMLKSPLHDLKACREELQGLAS
                     AGCEMVRVAFSDPSRAEALAELVSEAEIPLMADIHFNPDLAVLSLKAGVPAIRINPGN
                     MGSPRRLAEILHLARERRAVIRIGANGGSLSDAQIAESGGDRSLALVRAVEAQLKYLL
                     EHEFDDVILSAKSTCIQETVRANSLLAERYPYPLHIGITEAGPGLGGIVKNAAGIGIL
                     LNQGIGDTIRVSLTAPAEEEVKTGYSILNALGIRRKGFDLISCPTCGRKRIDVQALVQ
                     RILPSLSGLPDGFSVAVMGCEVNGPREAAHADLGISGSAGGFILFSHGRRIAVAEAGN
                     LEDILRETLEKICPKQN"
     gene            complement(59595..60632)
                     /gene="rseP"
                     /locus_tag="PLY83_00310"
     CDS             complement(59595..60632)
                     /gene="rseP"
                     /locus_tag="PLY83_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOV28510.1"
                     /translation="MIVSLFSFLLVICISVVIHELGHFLFSIRYGVKVYEFAFGMGPS
                     LFQKRQGETLYSIRVFPVGGFVRLAGMEEEQEGESAPPERTYPGKTPFQRFAILAGGP
                     FFNILLAIILTTVLLTFHGVLDIRTATVGSTISGFPAERIGLVSGDRIVSINDKPVTN
                     WDSMSATIRREAPKGPLSLKVEREGRILTFSVKAEIDKQSGIPLLGIRPRQIRYPLPE
                     ALFRSVGTAWEMTLSILRGLWTWAFGHQSVEFAGPVGIASMAGDAARQGWWSFLSFLA
                     VINLNLGILNLFPFPALDGGRLSFLIGEILTGRRLPPKWENLIHLTGFALLLALIVYV
                     TWKDILRLTHP"
     gene            complement(60629..61828)
                     /gene="dxr"
                     /locus_tag="PLY83_00315"
     CDS             complement(60629..61828)
                     /gene="dxr"
                     /locus_tag="PLY83_00315"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOV28511.1"
                     /translation="MRPQAGPPPGIAVIGATGSVGTAVLDVCRAHPDRFPVVALAASR
                     NVSRMAELAAEFNPPFLAMSDPDAARELALRVPVTTKVLSGPKSLEDLVLLPETGHAV
                     FASSGTSAIPALMSSLKCGKTVSLANKESLVIAGPWVMPLVQARDQLRPLDSEHNAVW
                     QCLAGERKGSVREIVLTASGGPFRNLDLASLEKVTPAMALRHPIWDMGTKITVDSATL
                     MNKGLEILEAMHLFGLPAERIRALVHPQSFVHGMVHFCDGSMKWLLSNPNMRIPAAVC
                     LGYPDRVELEGAHLDPAPLESLVLSFEKPDPRRFRCLALAIEAARSGGAAPAILVGAD
                     EIAVSAFLREKIPFLGISRLIEEVLAAFDEPSPQSPEEAIAIMEWARRKAAEILGESE
                     LSRRSTF"
     gene            complement(61825..62652)
                     /locus_tag="PLY83_00320"
     CDS             complement(61825..62652)
                     /locus_tag="PLY83_00320"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOV28512.1"
                     /translation="MEPSSRTFGSEIIIRALSGAFVVLAILGGILLGGIYWSFIVTLA
                     AMISLWEFYNLLSVKVKVTRGLGFISTFLLIAAATMNFSSAAILAITALIPFLVLCIE
                     IVRRQVLQNSHAIPNMGGTLVGLIFIALPWTFLVLLRSEPWGTMLLITLFACTWSCDV
                     TAYLVGVRWGRTYLCENVSPKKTWEGFAAGVAGSLFCAGLLAFAWQFPPFPLLLLGLL
                     CGLAGQIGDLGESLLKREAGVKDAGKLIPGHGGMLDRFDSILINATLAFLIFEVIWH"
     gene            complement(62639..63361)
                     /gene="uppS"
                     /locus_tag="PLY83_00325"
     CDS             complement(62639..63361)
                     /gene="uppS"
                     /locus_tag="PLY83_00325"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HOV28513.1"
                     /translation="MPEAPTDRCEALGKKIPSHVAIIMDGNGRWAKRKGFPRLVGHHA
                     GVRAVERTVRAANDLGIRTLSLYAFSSENWKRPENEVSGLMGLFRIYIRSKIGTLMAE
                     GARIRFAGKIDGLPADIQQIVRDAEEKTAGQKGLQLVICLNYGGRQELLDAMNRAAAA
                     GEPFPLDEVTLRKYLYLPDIPDPDLIIRTSGEMRLSNFWLWQSAYSEFYFTDKLWPDF
                     GPEDLAQALEAYSGRERRYGAV"
     gene            complement(63333..63992)
                     /locus_tag="PLY83_00330"
     CDS             complement(63333..63992)
                     /locus_tag="PLY83_00330"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOV28514.1"
                     /translation="MQAENRTIFSPTAEEIFSRREILWSRLQEEVSRCRLCPLAETRN
                     RTVFGDGPRETSLMLIGEGPGADEDEQGLPFVGKAGQLLTTILSAAGIDRREVFIGNV
                     VKCRPPGNRVPTLEEMMACDTFLQTQIALVSPRILVLLGSTPTRWILKTTAPIGELRG
                     KWFKWKGIEVFPMYHPSYLLRNQSRAVGSPKDLTWKDIREVKRRWDEVKKDAGSADGS
                     L"
     gene            complement(63976..64830)
                     /locus_tag="PLY83_00335"
     CDS             complement(63976..64830)
                     /locus_tag="PLY83_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV28515.1"
                     /translation="MQDDETAPISGVRVISFVGRAGTGKSQRAQVLARNLEIDFILDD
                     GLVIRKGQIVCGRSAKTERNQVRAIRRAMMEFPDHRQEVREFFKQAQPCTVMIVATSE
                     GMAAQIARRLGLPPPENVIRIEDVATPEEIRRARLERKVKGQHVIPVSQVQVRKNFAG
                     KLVGKLRVFWKGKNRYEGEKTIVRPPFSFIGALRIEPQAIEDLVRHVALRTPQVKSVQ
                     EVRIKPGSEGVSIQMKIGVILGDRNLVEIARTVKARISSSVSYFMGLDVRGVDVVVAE
                     VDLHAGGK"
     gene            complement(64817..65935)
                     /gene="hisC"
                     /locus_tag="PLY83_00340"
     CDS             complement(64817..65935)
                     /gene="hisC"
                     /locus_tag="PLY83_00340"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOV28516.1"
                     /translation="MSWKSHIVRKSLADVEPYRPGKPISEVQRELGLQSAIRLCSNEN
                     PWPLPEKVLEAVRASSEEICRYPDPASYRLKRAIAMKWGRSPSEVIVGAGTEGILYSL
                     FQSIIDEGDEVAYAVPTYPMYGLAAAAAGAKRIEIRTEWDGGGSCIDRLADACGPRTK
                     ALVICNPNNPTGTIMNRADLTALANSLNGRETLLILDEAYAEYVEDPKFASGIDLFDQ
                     LGNVVMIRTFSKIYGLAALRVGYAIAPKPVVETYLKVRKVFEVNSIAQNAALAALSET
                     AYIQEVRERTLAERERMSNALRDMGIHVQPTQTNFIILDFPNARTVFENLLMEGIIVR
                     LGDDLGFPGHLRVTVGLPEENDLFLQGLGKVLKRIAGR"
     gene            complement(65962..>66510)
                     /locus_tag="PLY83_00345"
     CDS             complement(65962..>66510)
                     /locus_tag="PLY83_00345"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048276.1"
                     /note="adds enolpyruvyl to UDP-N-acetylglucosamine as a
                     component of cell wall formation; gram-positive bacteria
                     have 2 copies of MurA which are active; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOV28517.1"
                     /translation="VTGGDVTVTGMEPEHLEALLAKLEEAGTRLSIGKDSVRTLPGGA
                     LSAVSLKTLPYPGFPTDLQPQIMAALCLAKGTSVIQERVFESRFLHVSELKRMGCQIE
                     LQGDTAIITGVEHLTGAEVRATDLRAGAALVLAGLAASGYTCIYQMGHILRGYEMMVE
                     KLQSLGGEVETVPCDENEGPKF"
BASE COUNT        13389 a        19390 c        19673 g        13958 t
ORIGIN      
        1 agggtttcgg ggcgagaaag cctgaccatc gcaccggaag ccggatcgga tcggctgagg
       61 ttccgttgcg gaaaggcctt taccaaccga cagattcttg aaaagctcga aatggcgtcc
      121 agcgaaggga tcaggaaagc gaagctctat ttcatggtgg ggcttcccac ggaaaccgaa
      181 gaggatcttg tcgcgatcgc ctctctcgcg gagtcgatcc ggaaagagtt acgcctcggg
      241 gtttcacttt ccgtgaatgt cttcgttccc aagccgggga cgtcctggga aaatcagccc
      301 ttcgtgggcg tagcggatgc ggaaaaccga ttccggatcc tgaagacctc cgtttcggaa
      361 agactgggca aaagctgcga tgttcgattt tcgagcctgc gtgaggccga acttgaatat
      421 cgcctttcct ggggaggacc tgaaatcgtg gatttgatgg aagcccttgt tgaaaaaaga
      481 agagggggga aaaccgatgc gtcctgaatc cgaagaatgg gaaatggacg aagacgaaat
      541 ggatcccgat gagctgcctg agcgcccgca gaggcgtgtc ccttcccgaa ggagtcgtag
      601 gagccggtct cgtcgaacga gtttcggatt tggccatttc atgcttccaa tcgtaggcct
      661 ggtggccgtc ggggtgcttg ttctcggaat ccgcctgttc ttcttcccgt ccgccagggt
      721 ggaacccact ccggctggtg tcgaacaaac cgaggcaacc ggttctgctg ccgtcaagga
      781 ggcaaatccc attccagcga gcggagaagt gcaagagacc gtcgtggccg ttcccgaggg
      841 gagtccgttg cagaaaatcg aatcgactcc gaaggcaaaa cccgcacagg aaaagccggt
      901 tgtcaaagcc gtcccggcga aggcctcagg ccaagcccct gccgagtcgg aagcgcctcc
      961 gtctcctcca aagaccgaag ccgtaaagcc cagcccgaaa aaagggacgg aaccagccgg
     1021 aaaggaaaac tggggggttc aggttggggc tttcaaggac cggggaaatg cggagaattt
     1081 gatcaagaag ctgcggcagg aaggctttga ggctggattg atcgaggtgg ccagcggtga
     1141 agcgaccctc acgaaggtgg tggttcaggc cggggatgac cggcctgagg ctgaaaaact
     1201 ggcgaaaacc ctgagtgata agggatatcc ggttctgatc gttaagacac gttaaaacgc
     1261 gcaaggtgga tggaggttcc cgggatgtcg ggttttgtaa aagagttgat ttcgaggaaa
     1321 gaagcgattg aatatgtctc ccgtgcgctg gatttcccct ggaccttgaa tactcgtgcc
     1381 gtcccccttc aggatgcaat ggggaagaga agcgggggga accttcttgc gccggtagac
     1441 tatccgccgt tcacgcgaag cacccgggac ggctatgcct tacggtctgc ggatgtggcc
     1501 ggagcctcgg attcggcccc ggtcttcctc cgaatcattg gggaagttcc gatgggagag
     1561 tggactgaaa agcggatcgg agaaggggaa tgtgtccttg tccacaccgg gggaatgatt
     1621 cccaatgagg ccgactccgt ggtgatgctc gaggatacgg ttcaggctgg aagctggctg
     1681 gaggttcgaa aggcccttca gtccggcgag aacctcgttt cccttggcga ggaaatcaag
     1741 caaaacgacc tgctcctgaa agcgggcgat ctcgtggggg atcgaaatgt cgggattttg
     1801 gcgacattgg ggatttgcac ggttgcgatc tacgatctga agatcggcat tctttcgacc
     1861 ggggacgaga tcgttcccgc ggaaacgccg tcccttccgc cgggaaaaat cagggacgcg
     1921 aatgcctggt ttctggaatt cctcctggcg cgacacggat tcccggcgaa acgcctgggg
     1981 attgtgccgg acatacgaga tcggctagcc gactcggtca gggaagcgat ggaagaaaac
     2041 gacatcgtgg tactcagcgg cggttcatcg gtgagcgttc gggacttctg ttccgaaatc
     2101 atggaaagcc ttcctttccc cggtcttctc gtccgcggga tcaacatgag ccccgggaaa
     2161 ccagccctca tcgcgggttg ccgggaaaac aggcgcctgg ttctgggatt gcctgggcac
     2221 ccgctgtcct gctccgtcgt ggcgatcact ttgctgctcc cgcttttata ccgactggcc
     2281 gggatcgaaa gtcccctgaa gaagatggag gcggttttgg ccggggatct tttcgggaaa
     2341 gccggtgtgg aggagttcat ccccgcgatc ctttctgacg gagaagtcct gcccatgccg
     2401 ggcaagtcag gatacgtctc cgttctgaaa gatgcaaacg cttttgtgcg actcgcggaa
     2461 aacgaggaga cgcggcgaaa gggagagaaa gcggaggttt tgcaatggtg agggcctgcc
     2521 aggaacacgt cagcctccgg gaggcaagaa agatcatcct tgaagcagtc aaggaggctg
     2581 ggttcccgag agttgtcaga atccctccgc aggaagggtt ggggttcgtt ctggcagagg
     2641 atgtcctttc ccgccggaac gttccgcatt atgtggcttc agccgtggac ggctacgccc
     2701 ttctggcggc cgaaacaggt ggggccactc cagcgacagt ggtccgactt ccccgggacc
     2761 gcttccagtg ggtgaatacc ggcagtccct tgccggattg gtgcgacgca gtggtgatgg
     2821 tggaggatac ctcaacggcc gagaacggtg atctcctcct ttcgaaagcc ctgccaccgg
     2881 gggcgaacgt ccgcccggtc ggggaggacg taatgaaggg acagattctg gcccgctggg
     2941 gcgaaagggt tgatcccccc ctcattgcac tcctcctggg agcgggagtg gaagaaattc
     3001 ccgtctatcg ccgtccgaag tgcctctttc tcccgactgg agacgaaatc tgcccgattc
     3061 aggaatgggc tgcaaccgaa gagggaccga gggggaaagt cccggaaacc aattcaacgc
     3121 ttcttaaggg catttttcag gaatggggac tggacctgga aacccatccc gtgttgccgg
     3181 atatagcgga cctgatcagg aaaaagctga aagcggcaac ggaaacgtat gatctggtac
     3241 tgatcggggc cggaacggcg aagggaaaaa gggatcacac cgccgaggtg atctccgctg
     3301 agggggagat ccttttcagg tgggttcgaa tgaaaccggg acgtcccgcc ctgttcggca
     3361 gggtcaagga ggccttggtc ttcggaattc caggattccc catgtctgcg gccgttacag
     3421 cctggagcct tgttgccccc gcggcgtttt ccatgtcggg catcccggtt ggcgagatgg
     3481 agacggaagc gctttttcgc cgagccctgg gggccttcgg ctccctgcgg tgcgaaatga
     3541 tgctccctca ttcgtccccc ccgggagtgg aagagtggct aagggtgaag gccgccgggg
     3601 tggaagggaa ggaccttatc tggattcttt cctccggggc ctcgacgatg ctgtcgatgc
     3661 atgaagcgga cgggttcgca gtcttgccgg cggaaacggt cgaatgccca aagggaaccg
     3721 gagtaaccgt gtggttccgg cagccggtgc cctggtcaag gagggccctt ttccagggct
     3781 cagacgatcc ggcgttcacc aggatcgtga ctcccgttcg aaaaaaaggg gccgacctcg
     3841 cgatccgtcc ggtcgggagc ctcggtgggc tcgcggcgct tgcgcgggga gaagggcatt
     3901 tggcggcctg ccaccttctg gatggaaaaa ccgggcgcta caacgatgcc tttattgaag
     3961 agttcgcaaa cggccagtcc tgggttcggg tgcttctgta ctaccgggaa cagggctttt
     4021 tggtggcgaa aggcaacccc agggagcttt cgaggatcga ggaccttgtc aaaaagggaa
     4081 tccggatcgt caaccggcaa ccgggggcag ggacgcgtgt cctcctcgac tggttccttc
     4141 aggaggccgg tttggaccgg tccagaattg ccggttatgc aacccaggcc atgacccact
     4201 tggatgctgc gagccgagtg gcttccgggg tcgtggatgc ggccctgggc atccgggctg
     4261 ccgccgacgc cttcggcctg gatttcgtga cgatggcctg ggaacctttc gaactggtca
     4321 ttccggataa gcacctggag cacccgggtg taaaggccgt tctagagtcg atccgggaac
     4381 cggagtgggt caggcaagtt gaagcattag ggggataccg atggccggat tgatgggttc
     4441 tttcaaccgg aggctcgatt acgtgaggat ctcggtaacg gatcgttgca actaccgatg
     4501 ccgctattgc atgccggaag agggggtcga ctggctcgaa cacgaccgga ttctgacgta
     4561 tgaggatctg ttggccctct gccgtttgct tcacgagatg ggagtcagaa aaatccgggt
     4621 aaccggaggg gagcccaccg tcagaaaagg cctgaccggc tttctccgtc gccttcacga
     4681 ggaaattccg gctttgcatg tggccctgac gacaaacggg tcccttctgc tttcgatggt
     4741 cgaggaactc tcctcgatcc ccctggccgg cctcaatgtc agcctggatt cgttggatcc
     4801 agaaaagttc aggtttctga cccgttgcgg ttcccttgac gtggtcctgc agggaatcct
     4861 tgccttcagg acacgatccc gtgtccccat gaagatcaac acggtcctga tgcgtggttt
     4921 caacgacggg gaaattccgg atcttctcaa ctttgccgga tcctgcgggg ccgtcctgag
     4981 gctgatcgaa ttcatgccgc tggatgattc cgtctggagc catgatcgat tcatctcggc
     5041 ggacgagatt ctcgagtcgc ttccggaccg tggaaagtgg atcccggaat cgaaagcccg
     5101 gaggagtcca aatcacggga ttccagaagg cccggcccga tacctggttc atgcggaaac
     5161 cggacagagg ctgggcctca ttgccgcagt gggacatcac ttttgcgaaa cttgcaaccg
     5221 actcaggatc accgcaacag gagagcttcg aagctgcctc ttcagccccc aaggagtttc
     5281 cctgagggag gcactccgca ggaaagaaat ggaaacggtc cgcgggctga tcgtcgcaga
     5341 agcgggtaaa aagccgaagt gctggaagga ggcttctgcc tcttccaggc acatgtcaca
     5401 gattggagga tgaagaatgg gagagctgac acacctggat agggaaggac acccggtgat
     5461 ggtcgacgta tccgcaaagc ccgaaaccaa cagggaagcc gtagcggaag gttgggtcca
     5521 tctgccggag cgcgtcttca gcgcgatcct cgaaaaccag gtccaaaagg gagatgtcct
     5581 gaaaattgcc gagcttgccg gaattcaggg ggccaagcag acggcttctc tgattccgtt
     5641 gtgtcacggg atccgtctgg atcaggcaag cgttcgctgt cagctcgtcc tggaaagaaa
     5701 agcggtccac gtggaatgcc atgtgaaggc tcgggaggtt acgggggtgg aaatggaagc
     5761 cttgacgggc gtttccgttg ccgcgctgac tgtctatgac atgtgcaagg ctctagataa
     5821 gggaattgaa atccgggata tccgcctcct catgaaaaag ggtggtaaaa gcggagaata
     5881 caggattccc gaaggggtgt gaaggatgcg ggtcatcgga atcgtggagg ggttaaacaa
     5941 ggcggacggg ctttcgatgc tctatcttca cctggggagc aagggagagt gccgggtgaa
     6001 cgggaagcct gccgaagttt tcgcttcggg gcctggtttt atggaaaggc tgagagaggg
     6061 gcaatggcaa atcctattct cttccgattg cccgatgaag gaagggcttt ttgtcgcttt
     6121 cgaaaaggat gggccggtcc ttttgatcga ggagccgttc gaaacttcct tcaagctcaa
     6181 gccggtcaga ggaggttttt tgtctctttc ccagaaggct acccttctta ggccggtcaa
     6241 cgtgggcgtt ctcaccgtca gcgacaaggg aagccgggga gaaaggaagg atactgccgg
     6301 gccggccttg gaggagttgg tgactccgat cggggcggtg gtttccgcgc gggccattgt
     6361 tccggacgat cgagttttga tcgcggaaac gctcagggat tggacggaca gggaaaaact
     6421 ccatctggtt ctcgtcaccg gcggcacggg gctttccagc agggatgtta cccccgaggc
     6481 ccttcaggac gtggcggaca ggatcgtccc gggtttcggg gaagcgatgc ggagaaaaac
     6541 cgaacccctt tttgaccgct cgatcctcag ccgtgcgatc gccgtgacga gaggcagctg
     6601 tctcttgatc tcattccctg gcagcgaaag aggggcgcgt cagtgttacg aggccatcgg
     6661 aggggttttg cggcatgccc tcgatatcct gaacggctgg gaaggggatt gtgcggttca
     6721 cggacatact cacacctaga agaggagtcg tcatgccgga gacattgggg tcctttgttg
     6781 acaagatcgt gatcatcacc ggaatgtcgg ggggagggaa gtcgactgcg ctccacatcc
     6841 tggaagacca gggttttttt gcgatcgaca acatccctcc gtcccttttg ccgcaacttg
     6901 tcgaggtcct ggggaaacac cgggcggcgg tccttcacgg agtggccgcg gtggtggata
     6961 tccgaggtga cgatctcctc agggaccttg aagcggtttt ggagggcctc aggcgaaggc
     7021 tcgccagtgt tacgctgctc ttcatggatg catcggattc ggccctgatc cggcggttcg
     7081 aatgcacgag gaggcgtcat ccccttggac ccgatctttc gacgctggaa ggcatccaga
     7141 gagagaggga aagcctggct ccaataagag aagcctctga cgtggtcctg gatacaacgg
     7201 gttcgaacct gtccgaattg agggggcgcg ttctcgaaag ccttggaact cccccgggac
     7261 gcctgaccgt tttcctgact tccttcggat acaagcatgg aattccccag gattgtgatt
     7321 acctcgtgga tgtccggttc ctatccaatc cctattacga agccagtctg cgtgcccaga
     7381 gcgggaagga tccagcggtt gtcgagtata ttctcaaggc ctctcccgcg gcggtcgagt
     7441 ttttgtcgaa acagaaggaa ctcttcgagt tcatctttcc cctgtacgaa gggacaggaa
     7501 aaacacagat ccacattgcg gtcggatgta cgggggggag gcaccgttcg gttgccgtcg
     7561 tggagtggtt cgcctccagg ctggagcaaa cgggcgtttc gtgcgttgtc cggcaccggg
     7621 atatcgacaa ggagcagaca tggtaaacct ggagtggttg acgccttttg gaattggaat
     7681 tttgatcggc gggttcgccg tgatgctctg gatgcgtcgt tcaatccgga atcgacgggg
     7741 ccttgccgaa atcatgtcaa gcgccatcgg ctaccgccta gcggcagggc cccgtgtcgt
     7801 tgccctaggc gggggcacgg gactttctac gcttctgaag gggctgaaag cctacacgcg
     7861 gaatatcacg gccatcgtca cggtgacgga cgaagggggc agttccggtc gcctccggac
     7921 ggaatggggc gttctgccgc cgggagatgt ccgaaactgt atcatcgctt tagctgagaa
     7981 cgacagcgca ttgaaccgga tcctgagctt tcgctttaac aagggggaac tagcgggcca
     8041 tagcctgggc aacctggtgc tgctggcggc gaccgagctg gcaggggatt tccgcctggc
     8101 tgtggaagag atgaacaagc tcctcgcgat ccggggaagg gtcctgcctg ttacgacgga
     8161 agccgtgacg atcgccggag tggatgccaa ggggaaccga tatcgtggag aactggatgt
     8221 ggcgcgtcat ggccgtgagc tcaccgaggt ccatcttgag ccctcggacg caaggccttt
     8281 gcaggaagtg gttgagtcca tccgggaagc cgaggtgatc gtgctgggcc cggggagcct
     8341 tttcaccagc attcttccga acttgctggt gcagggggtg gcagaggcga tccgcagggc
     8401 cgaggccccg agaatctacg tggcgaatgt gatgactcag ccgggggaaa cggacgggtt
     8461 ttcggtctat gaccatgtgc gatggattaa gcaagtcctc gggcagtacc ccgatattgt
     8521 catggtcaat gaaacggaag tgccgaaaga catcctgcag cgataccggg aaacgggatc
     8581 ggagcccgtg atcctgaaag aacgggagga ggtacggctg gcaaaggcga aagtccaggt
     8641 gattcgcggg gattttcttg aaatcacccc ggaagggctc gtccgacatc agacccagag
     8701 ggtttcggaa gcgatcatga aagtggcccg cgaggcgaaa gagctgggaa gatgagcgcg
     8761 attgcagaaa agttatggga tgaatggctg accgctccag tcaatggcga gagggaggcg
     8821 gacgccgaaa tcggagcgct gatcctttcc atgaagggaa aggaagagaa ggacaaaacg
     8881 gccctttcct ccaggcgtct gttcctcttc aggcgcctgc atcgtctttg gaatgcaggg
     8941 acctggaagg aattcgggga gctgggatct ttcctgagat tccctccgga cctcaagggg
     9001 cacccggctc tcgttgtccc gcaatccatg atggaaaaag ccctggggat cctgcgctac
     9061 ctccaagaag gtccccctgg atgtcgatgg gcctggctga agggggtctg gggagggctg
     9121 gggtcccttt atcttcccca gacagggtat tacctggtgt tccggttcga acaaaagaaa
     9181 cggccgatcg aagagaaaac agcggccctg ctgaaacttt cgggaatccg ctacaccgag
     9241 cgtttccgtt ctttccatac cgaccttctg atccgggacc aggctcagat tgtgtctttt
     9301 ctttcgggcc tcgggctcta cgggactggt ctggccctcg aggaaaagtc cttgatgcgg
     9361 tccatgagga acagggcgaa taaactggtc aactgcgatt cagcgaacat ccggaaaagc
     9421 ctggatgcgg cggaaaggca gctggaactg gccgaacggg ttcgttcctc cggcttgttt
     9481 gaccgactgg caccttccta tcgggaactt gtaacgacaa ggctcgaaaa tcccagcgca
     9541 tcccttcgcg agctggggca aatgctttca agacccgtga cgaaaagtac agtagaatat
     9601 agatggagcc gcttggggag acttttggag aacaccgtga agggggatgg ttgccatgta
     9661 ccttgggaaa gctgatgtca acacctacga aaagggggcc gggcgcgagt atctcgtttc
     9721 caacggctgc gggagctact gtttctcgac ggtcgcggga gcgaatacaa gaagggaaca
     9781 cgggcttctg gtcgtccggc cccgggattg cgctcagcac agggtcctcg tcagtaaagt
     9841 ggaggagact ctttttaccc ggggcaagaa gtatcaactt tccaccaacc gttataagga
     9901 tgtcattttc cccgacgggt ttcgctacat ccaggaattc cattccaacc ccctgcccag
     9961 cattctgttt gtcgtccaca gcctgctcat gaggaaaaca gtgttcatgc cagaaggcag
    10021 ggacgcctgc attgtcaagt atgagctttt ggcaagcccg gaccggatcc agctggaggc
    10081 gagacctctg ttcgcccatc gcctgtatac agatgtccgg ccaaaatcca cggaagactc
    10141 ctttcatgcg gattatgccg ccaacgggat tctggtgaat ggccgcggaa tgagcagttt
    10201 tctttcttac tccaagggcg aatggcaccc caaggcattc tggcatgacc atctccttta
    10261 tgagaaagac gagattcccg ctccatcgac cgaggatctt tggtcgccgg gtgctctttc
    10321 gatcgagatg gaggaagggg aaaccgccta tctggttttc tccgccgaac ccttccatcc
    10381 ttccgtcgag cagatcgagg ctttcgagaa agaggcgatt gcaaacctgc agagccgttt
    10441 cggcaagctc gaacccgcag ggcagtcctc cgcggtgagg gacctgacgc ggacggcgct
    10501 tcacctgttt gcctcgggga aggaccgggg gatgaccctg ttctccgggt atccatccct
    10561 ttgcgaacgg gcccgggaca cttttatcgc ccttcctggg ctgaccctct caatgggacg
    10621 ctaccgcgac gccgaggaaa ttctcaatcg ttggctcgaa ttggcccgcc tcaacgattc
    10681 ggtgatgccg agcgaaattg atccttccac gggccacgcc ctcctgaaag ccgcggatgc
    10741 agggctgtgg ttcttctatg cgttggaaaa actgacccag aggacaggtt ccctggaatt
    10801 cgtggaaaag aactgggaaa tcctagcgga gatgatttcc cgttatgaaa cggcgattcg
    10861 agtcctcgac ctgcattgcg aggaggacct cctggttctt gagaagcctg acccggagag
    10921 gcactggatg gagggaaccg cgaacggcag ccccgttgtg gtccgaagcg ggaaactcgt
    10981 ggaattcaat gccctgtggt acaacgcctt gcgcgccatg gagcagttcg cagaagggct
    11041 tggcctgccg gaagaatcgg aaaagtacgg gaaacaggca gatcggaccc gggaagcgtt
    11101 tctccagacg ttctggaatc cggagggcgg attcctgttc gattccgttg acggggagag
    11161 aaaggatgat gcgatccgtc cgaaccagat cctcgcggtt tctctcccga gttcaccgct
    11221 acccccggag atcgggagat cggttgtgga agtttgctgg aacgagctcg tgaccactta
    11281 cgggctgaga actcttgatc cccatcatga aaaatacaag ggcaggttcg aggggagact
    11341 cgaccagaaa gagaaggccc gttaccgcgg gatggcctgg ccttggcttt tgggacaatt
    11401 tgtgacggcc tacatgagat ttaacccgaa gaggagcgat attgcctggt gcttcatccg
    11461 tccgtttacg gcacatttga ggcaggggtg cctggggggc attgccgagg tgtttgatgg
    11521 gagcatgcct tacctccctc acggcgacgt cctgagcgcg gcaagcatgg gggaaatcct
    11581 gcgggtcatg gaggaagacc tccttttgca gcagcggtga gcctgtaacc tggactgaag
    11641 aggaaagcct tccttgattt accggacgga acggattaaa atttgatagg atttgaaaga
    11701 aggggtatgg ccgaggctgt acccttttcg catgaatcct ggtcaaaccg aaagaggaat
    11761 tgcaagaaag gggaggggag caaaatggca aaaatcaggc ttggaatcag cggtttcgga
    11821 aggatcggcc gcctcacgct gagggctctg ttccagcacc ggcacgaagg gcaggtagag
    11881 gttgcggcga tcaaccacaa gccggttccg atcgaggacc tggcatatct tttcaagtac
    11941 gattcggtgc atcgaacctt tcccgggaat gtccaggttg aaggggacgc gatcgtgatt
    12001 gacgggacac ctgtcaaagt actggcgcaa gccgatccgg aaaagcttga ctggaagtcc
    12061 ctcggagtgg acattgtcat cgaagcgacc gggaaatttc gggacaaggc cggtgctggc
    12121 cttcacctga aggcgggcgc taaaaaggtt atcatcacgg caccggggaa agacgacgac
    12181 tgcacgatcg tgatgggggt caacgaaggg gattatgacc cggaaaacca ccacgttctt
    12241 tcgtgtgcct cctgcaccac gaactgcctg gccccagtgg cgaaggtcct gcaggaagag
    12301 ttcgggatcg aaaaggggct catgacgacc gcccacgctt acacaagcga ccagagcatc
    12361 ctcgatcaca cccacaagga tcgacggcgg ggtcgttcgg ccgccatgtc gatcgttccg
    12421 acaaccaccg gtgcggccaa ggccattggg cgggtcatcc cggaactgaa agggaaaatg
    12481 aacgggatcg cacttcgcgt accgaccccc gacgtttcga ttgtcgatct gacggcggtc
    12541 ctcaagaggg aggccacccc cgaagagatc aacgcggcga tggcccgctg ggcggaagga
    12601 ccgatggagg gaatcctttc cattgtcgat gaagagcttg tttccgtcga tttcacgggg
    12661 aattctgcct cgtcactctt tgatccctcc cagacgatgg tcgtcgggaa cctggccaag
    12721 gttctttcct ggtatgacaa cgaatgggga tatagctgcc gggttgtgga ccttgccaac
    12781 cttgtagcca ggagggggct atagcgatga aaatgaaggg cctgcccgaa acagggctgg
    12841 aagggaagag ggtccttgtc cgggtcgatt tcaacgttcc cttgcgcgat ggagccgttt
    12901 cggacgattc ccggattcgc gcccacctgg acacgattcg ccgactccgg gaagcgggcg
    12961 caagaatcag cctgatctcc caccttggcc gaccgaaagg gaaagcctcc ccggaattct
    13021 ccctccggcc ggtggcctat cggctcgccg agctgacggg ctgggacgtc ggattcgtcg
    13081 atgaatgcat gggccctctc gtggcggagg ccgttgcaga agccaggccg ggctcgattc
    13141 tggttcttga aaatgtccgt ttccatgccg aggaggaaaa aaacgacccc aacttcgcca
    13201 aagcactggc agagcctttc gacgctttcg tcatggatgc cttcagcgca gcgcatcggg
    13261 ctcacgcctc gacggaggga atcaccgcgt ttctaccgtc ctacgcaggg atcctcctct
    13321 gcagggaggt tgaaatcctc gggcaggttc gggatcatcc gaaacgcccg ttcatcctga
    13381 tcctgggggg cgcgaaggtc tccgacaaga tcggggtgat cggccatctc cttgaaaagg
    13441 tggataccct tgtcatcggg ggaggaatgg cctttacttt cctgaaagcg caagggggta
    13501 atgtcggacg ttcccttgtg gagcaggacc gaattggttt tgcagagaac cttcttgcag
    13561 aggccgctgc aaggaacatc acagtatacc tgcccgaaga catcgtggcc ggagaatcac
    13621 cggacacgtc cggaccgatt gagatcgtcc cctcgaaagc catcccggaa cacctcatgg
    13681 ggctggatat tggtccgaaa gctgcagagg cctttacccg ggcgatcgcc ggcgcttcca
    13741 ccgttctctg gaatggaccg atgggagtgt tcgaaaatcc gcactttgca gaggggaccc
    13801 ggagaattgc gaaaagcctt gaagaagcaa cggctatggg atgccaaacc gttgtgggag
    13861 gcggagacac ggcggccgcg gtgggctcca tggggtttgc ggacaaggtg tcgcacgtgt
    13921 cgaccggagg aggagccagc cttgaattct tcgaaggtct tcctctgccg ggaatccagc
    13981 cgtttgttct agattaaccc aaaggggagg gaacatggaa agaacaccgc tgatcgcggg
    14041 caactggaag atgcaccacg ggatttccgc cacagggaag tttctttcgg atctcgaggc
    14101 gaggatctca agggatgcag gggcaaaaaa agccctggag ggggcctttg tcgagatggc
    14161 gatttttccc cccttcacga gcctctggag ggccctggaa ggcaagcgat acttaccctg
    14221 gttgaaagtt ggggcacaga acgtccattg ggcgtccgag ggggccttca cgggggagat
    14281 ttccgcggac atgctgaagg aagcaggatg cgattatgtc attataggac atagtgagcg
    14341 tcgccagttc tttggtgaaa ccgacgagca agtccgccgg aaaattgatg cggccgtgtc
    14401 ggcgggtttg cgtgcggttc tttgcgtagg cgagaccctg caggaacggg agacagggga
    14461 aacaatggcg gttgtcgaaa aacaactgat gaaggccctg gtgggccttc cgccggatga
    14521 agtcggaagg tctgtctgta tcgcctacga acctgtttgg gctatcggca ccggtagaaa
    14581 cgcgacccct ggcgaagcac aggaagtctg ccaggccatc aggcgttttg tcgcggagcg
    14641 cttcggcgac gaagcgggaa tggctttgcg agttctttac ggaggtagcg tcaaaacgga
    14701 aaacgccgcg ggattattga gcgaacctga catcgacggg gccctcgtgg gcggggcttc
    14761 tttgcaggca gactccttcg cgcagatctt gatcgcggct ttgtagggta ccgtttgctt
    14821 gaaaaacgcc cccgggaaac cttgacaacc gggggcgttt ttttggtttt caggaaataa
    14881 tgcaacataa gatacattat aggacaatcg attggggcaa aaaaaggagg cgttcaatgc
    14941 gccccttttt tgtcccgttt tcgcgttgcc cgattggttt ccagaaaggt gttacagggg
    15001 cagatcctca ccgatttcga ccgtttccgc atcgccccag attcgttcga tcccgtaata
    15061 gtcacggcct tgccggctga aaatgtgaat caccaggttc ccggcgtcga ccaggcgcca
    15121 ccgcggactt gaggctcctt ccagccgtga cgggagtccc agctcctcga gggtgtccaa
    15181 ggtcgtttcc gtcaaggtgt tcatgtggac atcggaattg gcggtggcca ggataaacgt
    15241 gtcggccaac gttgtggttt ctccaaggtt cataacggtg attttttcag cgtgcttgtc
    15301 ggacagcgat ttgacgatcg tttgaatctc tttgctcatc ttctccttca atcatgcgtc
    15361 acctcatccc tataacaaat aattaactat agattccgtt tgtattgttt taaccttttc
    15421 agaattatat cataatcttt cccgacgatg atcgttgcat gggtggctcc ccggtttgca
    15481 cggacgaggt tggctggaac accgcagaga tcccccagcg cctttgcatt ggaaagaacc
    15541 tgggccgttg ggttctccgg gtacgcaatg ttggtgtatc ggtaatcgaa atgcttggca
    15601 ttgcctagga aagccacgtc gatcccgaga cgctgcaggt gttccgaaaa agcctggctg
    15661 acccctttga ccccgcaccc attgaggacg gcaatctgtc ccttgattcg atccaggatg
    15721 gcatcggcgg gaaccagggc cgttccttcc gggctttctg ccttgtcccc gctgacagga
    15781 gccggggggg ttgtcaaata ctgtgaggca agagcgatat cccccagcca gtagctgatc
    15841 ccgtcgatca ttgcagcttt tcccggcatt gtctcgaagg aaaaccggcc ccggtcaagg
    15901 tccttcaggt agttcaccat ctggatggcc tgagttggct ggatctccgt ctggaagaat
    15961 cgcgtcgcct cctccacgat cttcggaagc ctggcgacgg tctccggttc tttgagcttg
    16021 ttcaggactt cctttaggaa aagctgctgc cgctgaatgc gaccgatgtc cccgagtgca
    16081 tcgtggcgga agcggacaaa ctccagtgca gttttcccgt tgagtgtctg gcgccctgca
    16141 ggaatgtcga tgtagagtcc gccggcccgg tccgtgtagc gtagccgctt ggggacgtcc
    16201 accgacaccc ctcccagaag gtcgaccagt tttgggaagc tctggtagtt gacgatgacc
    16261 gagtagtgaa ggggaatccc gaggaaattg gagatcgtgg aacgaagaag gtcgattccc
    16321 ccgtaggcat aggcgtggtt gagcttttgc cagccatgcc cctcgatctg gacccgcagg
    16381 tcgcgcggaa gggataggac cttgacgaac cggtcatcga tattgattgc cacgacggcg
    16441 atcgtgtcgg aacgatggga tccctggaca tcgtcgattc ccacggcgag gatattgatg
    16501 ttccccgtca gagtgtcgtg ctcgaaactc tcttcgattt cctgtgatcc tggggatacg
    16561 atcgtgtgca ttcgccaggc gattcccagg ccgatcgaaa gggcacagag agtgaaaaag
    16621 agagccaggc gggctttttt caagcctttt caccttccgt ttcgaatgta gaggtttttt
    16681 ttgaagatgt agtgttccac ggtccagggg aggaggtacc tcgtactctt cccctccagg
    16741 actcgatccc ggatttcggt gcttgaaatg gcgaggagcg ggatttcaag ggggatgatg
    16801 gccttctgga tcggttcggg caattccttc aggcgggcgg agttgtagcc gggccggctg
    16861 accgccacga tcctgcagag ctcgatgatc gcgtaaggtt ccttccagga aaggatatcg
    16921 aggactccat cgaggcccgt gataaagaaa agttccgtgc ttcccggggc ataccagtgc
    16981 cgcatttccc tcagggtgtc gacggtgtgg ctgtcgcccc cgcggtcgat ttcaagccgt
    17041 gaaaccttga aatgggggtt gtccagggta gccagaaggg tcatcgtgta gcggtcctcg
    17101 ggtgaggaaa cgagttttgt ccgcttgtga gggggatttc ccgtcgggac aaaaatcacc
    17161 tcggaaagcc ccagggcgaa gtaggcctct tctgcggaca ccagatgccc ataatggatg
    17221 ggatcaaacg tgccgcccat gattccgact cgattccggg caagaaaggt ctggttcagg
    17281 ttcctctccc ctttcgaaat caggcttccc attcgtcgcg acttgcgggg gcttcgatcg
    17341 ctttttccgg tttgaaggtg aattccgttt cccctatgct caccaaatct ccttctttgg
    17401 caccggcttc ctcgagcaac tcttccaccc gatatctcgc caggagcctg gaaaatcgaa
    17461 tgatcgcctc gtcctgttca aaattgtacc ttaggacggc ttcttcaagc cggggatgga
    17521 tcactcggta cccccggttc gctcggagtt ttacgatctg gacgacatcc cgtttcctcg
    17581 aggcggaagc ggatcccggt tcttcctcct gcagggcgac cagccgggtt tctccagtcg
    17641 ggcgcgggat ggaccgcgaa cactccacga tcgcctttgc caggtttccg atcccttccc
    17701 cggtcagggc gctcagcggg aagaaacgca accccatcct ttcaaaaacc gcacggattt
    17761 cctcaaggac tgaccggttt tctaaaagat cgatcttgtt cccaacgacg agggttggag
    17821 tgtcgagaag agcttccttg tattgcctga attcttcccg gaccgtttcc cactgtttca
    17881 gaacggatga gatgcctgct ccatccgaaa cgtcgaggac gtggaccaga aaccgcgtcc
    17941 gttcgatatg gcgcaggaaa gccagcccga gcccgagatt ccggtgggat ccctcgatga
    18001 gcccagggac gtcggcgagg atgacccggt catcgtcaac ggacagcacg ccgagattgg
    18061 gagaaagcgt cgtgaaaggg taggaagcga tcttgggctg ggccttggaa agggccgcaa
    18121 gcaggctcga tttcccggca ttgggcatgc cgacaagccc cacatccgcg atcagcttga
    18181 gctcaagtct cagggtccgc tcttctccag gcatcccttt ttctgaaaac ctcggggttc
    18241 ggcgggaaga actggcgaag accgcgtttc cccttcctcc ccttccgccc cttgcggcaa
    18301 gaaaacggtc acccggttcc acgaggtcgg cgatcggttc cggggaggag ggatcaaaaa
    18361 ccagggtccc gcaggggacc gggatcagga gatcttctcc cgaccgaccg tttctccggg
    18421 ctccctcccc cggtttcccc gattcggcga aaaagcgccg ctcaaactcg aaatcggcga
    18481 gcgtctgcag cgagcgggtg gcttcgaggt acacgttccc tccgcgtccc ccattccctc
    18541 cgtccgggcc gcctttcggg atgtactttt cccttcgaaa gctcatgcag ccatcgccgc
    18601 cgtttccaga acgaacggtg atggtcgcca aatcaatgaa tttcaatcgc aacacctcat
    18661 tgcagaaacc ttttgaaagc gaaaggggcc ctgcaaggcc cctttcgctt tgcaaatctc
    18721 agcgcagaaa gatctcgaat caggctggga ccgtttcaat ccgggcttcc ttgcggccgc
    18781 accttgtcgt gaaacgcacg attcccgact ttagggcaaa aagcgtatca tccttgcccc
    18841 tccccacgtt gaggccgggg tgaatctttg ttcccctctg ccgaacgagg atcgttcccg
    18901 cgttaacgac ctggccgtcg tgccgcttga ggcccagccg ctttccgatg gaatctctgc
    18961 cgttggtact gctgctctgt ccttttttgt gagccatcgt tttcctcctg tacccgaacc
    19021 ggcgctaccc gcgaaccgca tcgatgcgga cttccgtgaa gtcctgccgg tgtccgcgga
    19081 atttgcggta gtttttcttg cgcttgtaat ggaaaatgaa caccttcggt tcccggccgt
    19141 gccggaggac cgttccctca acgttgaccg tctcgaggaa gggagttccg atcttgaccc
    19201 cgttttcgtc cgcgaaaaaa aggactttgc caaggctgac ggacgccccg ggctcggcgc
    19261 tcatcttctc cactcggatg atttcgcccg gcgatacgcg atactgcttc cctcctgttt
    19321 caatcactgc atacatcgaa tcgttcctcc tcccgctggg ccacagggcg gccttcggca
    19381 cttgagccct tcccaagcga tatgttgaca gcttttgaat tttaaggtcc ctgcggcggt
    19441 ccgtcaagct tttatgttgt ctctttcaaa agggcgactg catgttcgag ggcttccgga
    19501 gaatcgggag tgcctgaaag cattcgggcg atttccgctg ccctttcctc ccccgtgact
    19561 tccgaaatct ccgtcaggtc cccgttgcgg cggacggtaa agtgctggtc ggctcttgct
    19621 gcgatcactg cctcgtgggt gacgaggatg acctggcacc ggcctgaaag ttcccggagt
    19681 ttgaatccgg tcaaaagggc tgaacgcccc cccagccccg cctcgacctc gtcgaaaaca
    19741 agcgttgggg gcaattggtc gtccggaagg gaaacctgga gggccaggag gatccggctg
    19801 agctcaccgc ctgaggcgac acgggcgatc gggccgctga aattcccatt cgaaagggta
    19861 aattcgattt cgtcagaacc gcttggccgg atttttccga ggtctttcag ggaaaccccg
    19921 aaatggaagt tttccatcgc aaggtcggca agatgcgatg tcacggtttc ctcaagtttc
    19981 gctgctaggg cccgcctgtt ttgtcccagg gcgttagccg tctgggcgac ttctttcctt
    20041 gcctgccggc aaagctgaac aagggattcc ctttccatgg agctttttcg catccagtca
    20101 aggtttttct cggccttttc gcaaaaagcg agcaattcat ccagcgattc gacgcccacg
    20161 agccgtttta gcttccgaag cgtgccaagc ctgttttcaa ggagctctct ccgctcttcc
    20221 gcctcggcca aacgcctgtc cagaaggagc gtcctggcaa aactctccag tttttgaatt
    20281 tccttcacga gaatttcgaa cgagtttccc agggccgctt cctgattttt gtcaagcagt
    20341 ccggccattt ctcggatcca ccgttggaat tcgtctgcta ggccttcttc ggaagcgccg
    20401 ccaacaaaac ggttcgcgat ttccagcaat ttcttctggc tggaaaggaa ttgttcgacg
    20461 gactcgagtt cctctttcca tccagcctcg ttgccttcct tcagctggag gcggcgtacc
    20521 tcttcaatga ccttttccgc gtccagaaag gttcgttcga cttcccgtct tctatcctgc
    20581 aaggccgaaa gttctttttc cagccccatc gctctttcga aatccagggc cagcttcctt
    20641 ctcaacctcg aggattcctc ccctccgcag ctgtcaagaa gatccctctg gcggcggggg
    20701 tcaagcaggt caagctggga gaactggctc tggatgccga tgcggctttc cgtgaaatct
    20761 ttcaatcttg cgaacgggac cgtttcttct cccagaaagt tccggttttt cgtccctctc
    20821 gagaggatgc ggcccgcgac gatttctttc ccggaatctt tctcccggaa aattccccac
    20881 acttctcctt cctcggcacc gaaacggatg gaagccgttt gccccctttt ccctgctagg
    20941 agctctaagg ccctgaccag gctgcttttc cccgccccgc tttcgcccgt tacggcgatg
    21001 aaattgccct ttagaacaag acgggcctgc cggatgcccc cgatattttt gacccagaat
    21061 tcggaaagct cccctttcgg catgttcatt cgatgacgtt ctgcccccac tggaatttct
    21121 ccagaagaag atcgtaatag tctcggtccg gcaaaaggat gatgctcacc gatttctcct
    21181 ttgccagcga tgcgcagatc tggtctcctg gtaaaagctc gtacccgagc tgcccatcct
    21241 gtgtcagcat catgtcccgg tggtctcccc gcgggatgat ccggatgaga tcgtcctcgc
    21301 cgaaaaccat gggcctggca tagagggtgt gagcacagat cggtaccagc agcatgcagt
    21361 gcacgtgggg tgggatgatg gggcctccgg ccgaaagggc gtagccggtc gatccggtgg
    21421 gggttgaaag gatcagtccg tctccggtca tcaggctcaa gggacgctgc ccaaccgaga
    21481 tttcgagggt aatgacccgg gcaaaagcgc cttttgtcac gacaaggtcg ttgagggcgt
    21541 agacctcgtg gaccatcgcg tctttgcgaa atacggtccc cttcagaagc tgccttctct
    21601 gggtgaagaa acgtccctcg aggatcgttt caatatcctt ttctgcccgg ttcgggtcgc
    21661 ccgaggccaa aaaacccagc ctcccgacgt tgatcccgta aagcgggatg tcgtttccga
    21721 gcacgtacct cgcggctctg aggaaggtcc cgtctccgcc gaggacgaca gcgaaacgaa
    21781 cttgctcttt ccagtcctcg tcctctgcac ccgggattcc aagaacagcg gcttcgtggg
    21841 gtggagcgag gaatgaaacg cccttttctg ccccccaaag aaggagacgc ttgccgattt
    21901 ccagagcctt tggcttttgc gggttgatca gcaggccgat tttgtctttc atcgttcaat
    21961 cccctttttc tgtgccttcc cgttccggtt ttccggatag ggccagaact gcttcagaaa
    22021 cgacggctgc caccgtttcc ttcacgtttt caagagtttt cccctctcgg gggacccgcg
    22081 agagatgcgc aaggaattcg atattcccgc tcggcccctt gatgggggac caggtcaacc
    22141 ctgccaggct gattccaggt aggccccgga cgtaatcgat aaattcctcg agcacctccc
    22201 ggtgaatttc tggggaacgg accactcccc ccttcccgat acgttccttg cctgcctcga
    22261 actggggttt caccaggacg gccatctctc cgtcgggttt cagaatgtcc agcagaggcg
    22321 gcagcaagag acgcagcgag atgaaggaag cgtcaaccac gatcaggtcg atcggcgtcg
    22381 ggaatgattc ccggctaagg taacgggcgt ttgtccgttc catgacgacg acccttggat
    22441 cggtccggat tttccaggcc agctgtccgt atcctacatc gacggcgtag actcgggaag
    22501 cgtcgcggga cagaaggacc tcggtgaagc ctccggtaga agccccgaca tccaggcaaa
    22561 cgagcccggc tggattgatt tcgaaaacat ccagagcctt gatcagtttt ttcgccccgc
    22621 ggctcaccca ctcttcttcc tgtccgatga ccgtcaggtc aagatcaacc ggaacttgtt
    22681 gtcccgcctt gtccgcgggg gatccgttga cccgaacgag tccagccatg atcagcgcct
    22741 gtgccttggc acgggtcggc gcgagtccct tttcgacaag cagcttatcg agccgttcct
    22801 tcgagcgagt caagagcctt ctccaggatc tctttcgccg taagtccaca ggctctccac
    22861 tgggctgcaa ccgtggcttg cgggatgtag cggtcaggaa caccgaaagg gagcacccgt
    22921 ggagaaccgg gaatcgaatt ggcccgggcg gcgatcgcct caccgaaccc cccgcagacg
    22981 aacccgtctt cgatgacaca ggctaaacgg ttgcaggaaa gaatcgtgtc gattcggtcc
    23041 cagtccgctg gccgtacaaa acggaggtcg agcacggaag gctccggcag gccttcccgc
    23101 gctgccaatt catggatttc cagcgccagg gagaccgctt ttccgtaggc catgatcgtc
    23161 cagggggccc ttccttcgat cagcacttct gctttccccc agtcctgcgg ggcgtcaggg
    23221 ttgcccgaag ccttcagccg atccggggcg aggccgcggg gaaaacggat cattgctgga
    23281 tgtccctgcc gatacgattc ccggatcatc cagcaggctt cttcggcatc tcgcggggcc
    23341 atgatggcca ttccggggat cgactttccc cagcagacat caagcagacc gtggtgggtt
    23401 tctccgtcct cccccaccag gccggcccga tcgacgagga gaaccacggg aaggtcctgc
    23461 atggcgatgt catgaatcag ctgatccatg gccctctgca aaaacgtgga atagatgaag
    23521 acaaagggcc tcattccgcc ggcggccaag cccgcggcat aggtgaggag atgcccttcc
    23581 gcgattccca cgtcaaaaca ccgattcggc cagcgttctt cgaaaccgtt cagccgtgtc
    23641 ccgtctttca tggctgcagt caggcaaacg atgcgttcat cggtttcggc cagttctctc
    23701 acggaatggg cgacgacatc gctccagctg gtccccgaag gtttcgtttt ttgcgccgtt
    23761 tttcccggga tcccatggta tcgcgtcggt tcggcttcgg ccggcgggta gccttttccc
    23821 tttttcgtcc gaagatggat caagagcggc tgcccataaa accgggaaag attgaagatc
    23881 tctagcagtt catccaggtt gtgcccgtca aaggggcccc agtagctgat tcccaggccc
    23941 tcgaagatgt tgttgggctg cagcagggac ttcatccgtg tcttggagcg ggagagcaga
    24001 tcctcgatgg attcccctcg ctgccttttc cggcactggt cttttacgta ctccttgaag
    24061 cgcttgtaat agggattgac cgctagccgg gccagataat cggcgaggcc cccgacaggg
    24121 cggctgatcg acatcgagtt atcgttgaaa atcacggtca ggcggctttg tgtttccttc
    24181 acattattca gggcttccat ggcaagtccg ttgtaaagcg ccccgtctcc gatgaccgcc
    24241 accacctcgt ggttctgccg catcagatcc cgggccttgg catatccaag ggcggctgag
    24301 attgacgtgc tgctgtggcc tacatcgaaa tgatcgaagg ggctttccct tcgtttgggg
    24361 aaaccggaaa ttccgccttc caggcgcagg gtcggaaagc gttcgaggcg gtccgtcagg
    24421 attttgtagg catacgcctg gtgccccaca tcgaagagaa tgcggtcttt cctgggatcg
    24481 aagacgcgaa gcagagaaac gatgagttcg acagccccga gtgaagatgc caagtgtcca
    24541 ccgttttctg taacgacacg aaccatcagt tcccggacct cctggcagag ccccggtagc
    24601 tgttcctttg gaagttgcct gaatccctcg taattcttca ggttctcgag aatacccatc
    24661 cttctggcga ttcgcctccg ttcaactgga tcgatcaagc aggacgcggg cgagggaacg
    24721 taggggatcc gcttccggtc cgaagcagga aagggactca atcgccttca ggctctcatc
    24781 gcgggcgatc ctgcgggcct tttccatccc gtaggtggaa acgaacgtaa tctttccctg
    24841 gacagaatcc ttccccggag tcttcccgat ttgttccgaa ttccccgtga catcgaggat
    24901 atcgtccacg acctggaagg ccaagcccaa atgtgtccca taattatata atgcttccat
    24961 tttcacgtct tccaaaccgc cgagaattcc accagccagg acggaagctc ggatcagggc
    25021 cccggttttc agggaggcga ttttccagac aaaatccact tctgattccc ggcttgaagg
    25081 gtcggtgtcc aggacctggc ccgcacagac gcccgagggc ccgattgcct tcgcgaattc
    25141 gctgattgcc ccgaggatcc gttccgctgg aaaaacgggc agaagattca aaattgcggt
    25201 ttcgaacgcc cagagcagga gggaatctcc agccagagtg gcgagccctt cgccgaaaac
    25261 catatggttg gaaggcttgc ccctccgtag cgggtcgtta tccatgcatg ggagatcgtc
    25321 gtggattagc gaagcggtgt ggaacatctc gagggcaact gccatcggca gcaccgattc
    25381 cgccggacag ccggaaagct ccgctgcaga gatgcacagg aatggacgca ggcgtttgcc
    25441 ccctgcgcga agggaatacc ccattgcttc ccttagacgc tcggggatcc cagagtcttt
    25501 cccgagcagt ctttcgattt cccggtcgac ttcctcccgg catcgccgaa ggcgaagatc
    25561 caggtcctct tccctttccg tcatgacacg gcttcctctc cgctcaaggg atcccaggcc
    25621 gttcccccgg agttgtcgcc ctccgaaaga aggatcactt tctgccgtgc ctggtccagg
    25681 aaggccttgc acttttccgc cttcgccatc ccctcttcga acagcttcat cgcttcctct
    25741 agcggcatcg gttcactctc caggcgcttg acgatggcct ggagcgcttc gatttccttc
    25801 gaaaaactca tgggatgctg cccccctctt tccggtcgtt tgttcaaacg gatttgccaa
    25861 ccatactgcc gtatgataca atgccactcg ctgcatggtt tccaatctgg gagggatcga
    25921 cgatgtccaa ggtctgccag tgctgtggcc gcgggcccgt taccggcaat aacgtaagcc
    25981 actcgaaccg ccatacccgc cgtcgttggc agttgaacct gcaaaccgtt cgggtcgatt
    26041 ttggcgaagg aggccgaacc aagctcaagg tttgttcgcg ctgtcttcgt tccggtctgg
    26101 tcaagcgggc tgtctaaggg cagcccgcct tttttggttc gactccctct ccctcgtcct
    26161 atcgttccca ccagctgacg tagagaccca tctcaccgcc atccaggccc gcaacgatgg
    26221 gttcatcccc ttttgggcgg ttgctgatcg catacgggag ccccttgtcc agttctgttt
    26281 tctccagggg ccagtgaacc cctcggatgg aaactcccgt gcatcgttcc gagaacgcca
    26341 gaagcgaaat ggcttcaggt cttctgagct gtgtgagcgt tacggtttcc ccgccccgaa
    26401 tgaaggccag aagttccctg tgatcgacaa ggaccgcata gccatccctt tcacgaaggg
    26461 tcccaagaac ggatagcacg ttactccagg tatggtcgaa ccgtcctccc cagcaacccg
    26521 tcacgaggat tttgattctt cccagccgca tcgcgagtgt cttgagagcc atttggaggt
    26581 cggtgtcatc tttctcgcgg ggaagggaca gcacctctgc tcccctcccc gtcgcggctt
    26641 tccagagctt cggggaagcg ctgtcggcat cccccaggat cagcgagggc agaattccgg
    26701 cttccatgca ggcgttgacg ccccggtcaa tagcccagac ggttcgattt tcggcggctt
    26761 ccagcagcca gctgaccctg ggtttacgcc ccccggcgac cagtaggatt tcctcccctt
    26821 gagggatcat cggggaagac aaaagcacct gaggcagtct gatttcctcg actttcttca
    26881 tttgtggatc aactccctcc gctgaaggcc tgacggagga tctcctcggc cttgtcttca
    26941 tcaaccagaa tctccctcgg gcgagaggct tcctggggac ccaccatgcc cagctgttcc
    27001 atggtgtcga tcaggcgtgc cgcgcgcgta aaaccgattt tcagctgcct ctggagacgg
    27061 ctggccgaag cgatccccgt ttgcaggatg atcgagacgg cctcttccag gagcggatcc
    27121 tccaggttcg cagcgatttc tccccaggcc ccgttttcgt cgctgtcgat atcgatatgt
    27181 tgcggttcgc cgaaaacccg caggacatgc tggatgaaca aggagatccg gctttcgtcg
    27241 atccaggggc cctgtactcg aaggggcttc gggtagcggg aagtcaggag aagcatgtct
    27301 cctttcccga ggagcttttc cgccccggac aggtcaatga ttgtccttga atccgcctgt
    27361 gaaggaaggg tgaaggcaac ccgcgccggg atgttggcct tgatcagtcc ggtgacgaca
    27421 ttcacggaag gccgctgggt ggcgaggatg aggtggatgc ctgtggccct ggccatttgg
    27481 gcgagcctgc agatgtaatc ctcggcttct ttcggagagg tcatcatcaa gtcggccagt
    27541 tcgtccacaa caatgacgat gtaaggaaga cgatctttcg gcaggacttt cctgttgtag
    27601 gccttcaggt gccggacccg tgcgcctgcg aaaagctcat accggcgctc catttcccgt
    27661 atcgcccatc cgagagcgtg gacggccttt ttcgagtcga cgacgggggg ggtcagggtg
    27721 tgggggagct tctcgtagag agtcatctcc acccgcttgg gatcgatcag caggaggcgc
    27781 agttcatccg gtcttctggc gcagcagagc ccgatgatgc agctggaaac gaggacgctc
    27841 ttccctgacc cggtggtccc tgcaacgagg aggtgtggca ggtcttctag ccccatgacg
    27901 agcggcacgc cgtcgacggt aagtcctagc gggagaggaa gatcgaaatc gccgcgctgg
    27961 aagggggttg actcgaggag ggctcttaac ggaacgatcc atcttctcgg atttggcgtt
    28021 tcaatcccta caaaggtcgt tcccgggatg ggagcttcga cccgcagggc aggaaccgca
    28081 agcgccacgg ccagatcgtt gctgaggccc gcgactttgc tgaccttgat ccccggtgca
    28141 agctgaaggc ggaactggat gacggttggc ccggtgatcg tttctgccag ctcggcctca
    28201 accccgaatt cttccagggt ggagatgatt cgctccccct ggttgcggat cagctcgtcc
    28261 gaaaccgggt tgacggtaat cccctcattt ccgaaaagtt caagaggcgg ggggaaggcg
    28321 gaaggatcga tcccctcagg aagagtgtcc tcttcctgag aatccgccgg ttcgagcgct
    28381 tcttcaacgg gtaggacaag caggtcatcc gcttcctctt ccgcagcaga tggagagaaa
    28441 gtggagattt ccggcggctc agcaagttga gtcccacccg tggaaagaag cggggccgtt
    28501 gaaagttcag aaacgccctc cgtctgcctc ggaaaaagtt tacctgccat ggggagggaa
    28561 atctccgaca caaaatcttt gaaccggcgc attcccgaaa acagcccttc ccggatcgtc
    28621 gccgcccagt gcacgacgtc gagcggctcg atcagcccgt acagggctgc agaaaggaac
    28681 accccgaaaa gcccggcaag aacgatgccc aggacgcccg tttgtttcag gaggaattcc
    28741 ccgatggccc tccccagcag ccctgggttg agccaatcaa cggccgccag gggccaggca
    28801 ccctggccga tgaacagaac gacctccagg caaagataca ggacgatcgt tcccccggcc
    28861 tgttttgcca tgtcctggac cggttttctc aggaggtgga aaagcccgca gtacaggacg
    28921 aaaaaaagca ggagcacgac cccgccaccg gcaatcccga agaagccgtt tctgatggtt
    28981 tgcccccagg ctccggtcag gtccgtgtag agcgaagcca gaaaatagac ggcaaggaag
    29041 agcacaaaaa cgacggctcc gtcaatccag gccgccgttt ctggagaatc gccggatcct
    29101 ttccgcaccg tggagctttg tcgctcacga agttcgggtt cctgtttcct aaccctcgcc
    29161 atcgccgatt tcacaccccc ctaccagcag atcccgaatg agaaggctgg gttgcccatc
    29221 cgtgaccggc acgctctgtc catctttccc gcacaggccg ggttcaaaat ggaggtcacc
    29281 gccaatcgct tcgatctgtt tcagcgtttc cggcccgttg ccgatgaggg tggcaccccg
    29341 gacgggtttc ccgatcctcc cgttctcgat gagaaaggcc tcggtcacct ggaaaacgaa
    29401 atcaccggag gtcgggttga cctggccccc ccccatcttt gtcacgagca ggccttttcc
    29461 tgctttgtcc agcagggatt ccaagggatc ttcgccgggc agaagaaacg tgttgctcat
    29521 cctcggcaga ggtagattcc ggtaggaacc cctccttccg tttcccgtca gaggaagacc
    29581 cccctttttg gctgactgca cgtccgtcag atacgctttc aagacccccc tgtcgatcag
    29641 gatggtgcgt tgggccggtg tcccttcatc atcggactcg tagcttccat acgctctcgg
    29701 gatcgtcgcg tcgtccacga gagtgacgag cggacttgcg acctgctctc cgaggcgctc
    29761 gcggtaaacc gaatagtcct tctgcacgat atccgcttcc aggccgtgcc cgcaggcctc
    29821 gtgaatcatg gttcctcccg cctggccgga gaggacaacc ggcattcttc ctgccgggca
    29881 ctggctggcc tcaagcatga gcagggctct ttccagtgcc ttttggccga cttccagggg
    29941 gaacaggtcg tcccaaaaat tcccgggtcc ggtttgaaag gcgcgaacct cgtatccagt
    30001 ctggattgtc cctttgcgtt cgaccgtaac ctggaccgca aaaagggaat gaaagcggag
    30061 gtccgtaacg atctttccgt cgccgcggac gatgagggtg cgccgtccgg agaccttggc
    30121 ccggagagtc acctgccgga catggggaga agcgtttcgg atggatcggt cgacttcgcc
    30181 caaaggatct gtttccgggg ccttcagccg gaccggttcg aaatccgtat agcccgccgg
    30241 cgtgaaggaa agcgtgctga gggagggaag tccagccctg gcaagggttt ctcccatgca
    30301 aagccaggcg cccttcaggg aagaatccgg tgaatgcgaa aagacggagt gctcgtccag
    30361 aaggaccctt gccccgacac cgccggaagc accggaagac acctcctcga ccctcccctt
    30421 ttcgaacgtc acactgtgtc ccgaagcgga ctgcacgaag agatcgacgt agtccaccgt
    30481 tttgcccgaa agggctgcaa gctgttcgcc aagaaagttc cacccttcgg caaggggttc
    30541 aacgttcatc aatctcatct cctcggatca gcgcagcccg cgtcctgaat ttgaaaaaat
    30601 ggggcgcggc aacagaaaaa agtccctctc cccgccagag ggtgatccag ccgcaacccg
    30661 gaagcacaag atcctgtccc ttagagatct gaacctccgc ctcggttcga ggaatgccgt
    30721 caagaatcgt tcggcagccc cgacaaggga ttttcagaag tccccccgcg tgcttttcaa
    30781 ccagctccgc cgttctttgc agcttcgtgc ggtggggctt cagggattca ggggtgaagg
    30841 ctgcaatttt gaggtggttt cccttgggat aattcagaac tttgacttcg acaaggccgc
    30901 cgaaaagaag ggcgtcccca aaagacaggg agtacaccga gcgcacgatc tgtttttccg
    30961 gctggagcct gaaaaggcaa tccgggcaaa gaaccgggat ccacggatcg tccgaacgga
    31021 acccggggat gtcgacaaag gtcatgccca agcgcgaatt cgaaacttcg atggggccca
    31081 gagtggttcc cggcatcggc gaactcgagg caaaacgggc ttctgcgcag acgcttctca
    31141 ggaaggtcgt cttccctgag ttgaccggcc ccatcagcaa aatcctcgcc ccgctaggaa
    31201 agcgcctctc gagccacgca agggccttct tgacggcagc atgatccagc gcattcatcg
    31261 tgaggaattc gtccttcgag ctccccagaa tcgcttcgag tctcccgttt gggctccctt
    31321 caggaaacca tgatgcaagc atatcatatt tgttgataac tttcaggaat ggcttccccc
    31381 attccttcag ccgtttttcg agacggggat tgggggccag ctgcaaggcg tccagaaata
    31441 cgatgccgcc atcggcattc tccatctccc ggcgaaggat cccgaaaacc tgctcgtccg
    31501 agagcggggc cttcgtcgcc tggcggtaat ggactagacg gaagcagcgc ctgcaaacca
    31561 agccttggcg gggtttcagt ccttccggaa cgaagccggg cgcttctgga tcatcgcttt
    31621 gcaggaaggc tccgcagcct gggcagaccg ccggcggttc tttcccctgg aaaaacgtca
    31681 tggtttgcct tcaatcccct ttccatcctg attttcgtct cgggaggctg tcccctgtgc
    31741 ggccaggtgc tcttcgtaag tcctcgaaaa caggtggctt ccagagggag aaaggaaaaa
    31801 atacaggtat tcgtgctttt ccggaaacag cgccgcacgc caggccggct cggatggaat
    31861 gcagatgggg cccggaggaa gtcctttatt ccgataggta ttgtacggcg aatcgacttc
    31921 gaggtctttg taacttagcc ggttcagcgg tcgtccccgc tgtttccagg cgaagatgac
    31981 cgtcgcacag gattgaagcg gcatcccgag cctcaggcgg ttttcgaaaa cccccgcaac
    32041 ccttgcccgc tcactgttgt ctttggcttc ccgttcattg atcgaggcga gaatcgcccg
    32101 ttccctgatc cattgaggcg aaacccgctc gggcacccgg gaaccgacct tttccagcca
    32161 tttgcgggag gccaggcgga ccaggtcctt caaggcggtt tgcgatgggg tcacgaggta
    32221 cgtgtccgga aggagaaatg cgagacggtc ttcaggcttt tccggcaggt aactgcgcat
    32281 cgctggagga aaattggatg gatcagcaag ctcgcgcagg agaacggctt ctccacccca
    32341 agaggagagc cctgccgcaa gttccccgag ggaagtcccc gggatgagcc ggactccggc
    32401 ggagtccggc tcctgatgct gcatctgtcg ggctatttcc cagggcgttc ccgggcggat
    32461 cctgtaaagg ccaggttgca gtcgtttatc cattcccagg agagaaaacc agcgggacag
    32521 aacccgcgca tcgccgctcg ttacgccttg ctccaccagg gatcgggcga cagcaagcgc
    32581 attctgcccc ggctcaacca gggcttcgac cggggcccct ttgccaagag ggacattctt
    32641 ttcccaccac gccccatgct tcgaaacgat cgttccgaag agaagaagcg tcaggagagc
    32701 aattccaact ccacccgatc gaatcaaagg cagggttttc tttcctggct tcacgtttgt
    32761 ttaagaaaac ctcttctgca tttcggcctc cgcgacaatg gcggaagcct cgaccttcga
    32821 aagctcgaaa cggagctgcc ttcgggtttc ctccacgaag gaggggtcac tgattcccct
    32881 gaggttgacc tcgacattgt aggctgcggc atgggcagct gcccatgcga gccttcccgc
    32941 ggaaccgaca tcggtgaccg cattggggtt tcccttgaaa aggagcgatt ccataatgct
    33001 ggccgtttcc gcgactgttc gcaccgtttc aagggggacc ctgcaggcgg acttcagggc
    33061 ctcttggatc gcctgttccc tgagggactt ttcttcttcc gtgcttttgg gcatcctgat
    33121 cgcttccatg acgtggttgt atgcctccgc gtcctggtcc atcaattcga tcaatttttc
    33181 cgtaagctgc ctgctttttt ccccggcttc ggccatttcc ggccatacgt cccggaaacg
    33241 ttcctttccg gcggtcaaag ccgcgaccat cgaacagagg ctgctcgcca gagccccggc
    33301 cagagcagcc gccgaaccgc ccccaggagc tggagaggcg gaacccaatt ccttgacaaa
    33361 atctcgaaga gaaagatctg aaagcataag agactccttt ccaaatcaaa taacgcgttc
    33421 tgcaccctta taaacggcct taacgggaga tcctcccgca tggtacgcaa ggatcgccgg
    33481 ggtctgtccc tcgaggataa gaaaatccgc ctttttccca ggttccaggc tgccgcagga
    33541 cttctcccgc ccgagcgcgt aggccgcatt caaggtggtc gcggtcagcg cttcttccgg
    33601 ggaaagtctc atgcccaaaa cggcaagacc gaacacgaag ggaaccgact cgcagaagca
    33661 ggatccggga ttgcaatccg tcgccagagc gacgggaacg ccgaggcgga tcatttccct
    33721 tcccctggcg aagggtttac ccaggctgta ggccgtcgcc ggcaaaagga ccgcgatggt
    33781 tccggcagcg gccatggctt tcaatccttc atcggaagcc gcgagaagat gctcggccga
    33841 cagggcaccg acagaggccg ccagccccgc ccctccgagg tcgtggacct cgtccgcgtg
    33901 gatcttgatg ccaaacccca aggctcttgc ggcttcgaga atacgcctgc tttgagcgat
    33961 tgaaaagacc ccttcctcgc agaaaacgtc gcagaatcgg gcgatcccct gggatgcgac
    34021 ctccggcagc atctcgtgga tgatcatctc gacgaaagct tccgaatcac cggcgtattc
    34081 ttccggtatg gcgtgggcgc ccatgaaggt cgggatgaca tccaacggcg actgtcgccc
    34141 cactcgatcg atcacccgga gcatcttgag ttcagcctgc gtctcaaggc cgtatccgct
    34201 cttgatttcg atcgttgtcg tcccgtgagc cagggcggat tgggcaagct gaagcgtcga
    34261 agcgaagagt tcctcttctt cggccgcccg tacccgcttc actgaagaga ggattccccc
    34321 gccgcgccga aggatttcaa gatagggaat gccttccagc cgcatggaga actcggattc
    34381 ccgcaagccg tggaagcata gatgagtgtg ggggtcgaca aaacccggga tgacgcagtg
    34441 ccccccgcaa tcgatttcct ggtctggggg caggtgcctg acggcctcca ggacctccgg
    34501 ttccgggccg atctggagga ttcttccgtt ctgggtcagg atcgcaccct tagggatctc
    34561 ccggatcatt ccctggtcat ttcccgacag gggatggccg ggatcgatcg gagtgaagca
    34621 gcacgcgttg acaaatagct tgaccgtcat cgggcttctt cgctccccat cgtttccaga
    34681 agcttcagtt ccaggacctg catagggtca aaatccgcca cctggaggta ataggctgcg
    34741 ctttccagga tggcccgggc cgggatcatc ccgtagattt ccgtttccgc gatctcgacc
    34801 ccccaccggc gggcttcgaa ccggatcatc tccagcactc gataaagtgc gttcttttcg
    34861 taatcgacga tgttcatgct aacctggacc attcccttgt cctcaagggc aacgccgatt
    34921 ccctttacgg cacagagtcc tccgctcgaa gcccggacgg ctgcagcgat ccgcttggcc
    34981 acggccagat ccgtggtccg gaggttgacg ttgaaggcca cgaggaattt tcttgctccg
    35041 atcactgtcg ctccggcggt cgggtgaagc ctcggttcac cgatgtcggg gctgcgttcg
    35101 ggtttttcca cttcgttctt caggatctcg tattgccctt ttcgaatgac ctccaggcgg
    35161 gtcctctccg gcctaagggc cgactcttcg taaaagtaga ccgggatccg ggtcttttcg
    35221 tataaccgtt tgccgaattc atgggagaaa gccacgcatt cctccatggt catcccctgg
    35281 acaggggtaa aggggatgac atcgattgcg ccgatgcgtg gatgggctcc ctgatgatga
    35341 ttcatgtcga tgtgccggac ggcgacttcc gctgccaata ggagggattc cgcgagcgcc
    35401 tcgggctctc ccaccaggct cacgaccagg cggttgtgat cggggtctgc ccggtagtcg
    35461 aacagggtgc atccggcccg ttttcggaac ggagtgacga tttcctcgat gacctccggt
    35521 cgtcttccct cgctgaaatt cggaacgcat tccagaagtt tcctgctcat cagaaatcag
    35581 gcatcctttc caagagttcc gtccagggcc tttttgacgg tttccctgac caggtcctcg
    35641 tcggcaatga acggcagggt gatgtggtcg gttcccgggt gctggcggtt gtattccatg
    35701 ctggtggcca gcgcgttttc gtttcgtgcc caggccctcc gggccacccc ccccatgacg
    35761 tcccaagcca gggaaagttc gattgcccgg tcggccttct cgcttccgtc gagcaccagc
    35821 ccgaacccgc cgttgatggc tttcccgatc cccactcccc cgccgttgtg cagggccacg
    35881 agactcatcc cccgggcggc gttgccggcg aagcagtgcg tggccatgtc cgccatgatg
    35941 gcgctcccgt ccttgatgtt ggcggtttcg cggaaaggcg aatccgttcc cgacacgtca
    36001 tggtggtctc gccccatcat gaccgggccg atctctccgc gccgcaccat ttcgttgaac
    36061 cgtcgggcga tccgcatccg tccctcggcg tcctggtaca ggattcgagc ctgggttccc
    36121 accaccaggg cgtttttctc cgcgtcccgg atccagatcc agttgtccct gtcctggccg
    36181 cgccggttcg ggtcgatgca ttccatggcc gcacggtccg tcttgacgag gtcttcgtgc
    36241 cgcccgctca ggcagaccca gcggaaggga ccgtagccgt agtcgaaaag catcggcccc
    36301 atgatgtcct ccacgtacga ggggaagatg aacccgtcca gttcgtcgcg gccgtttttc
    36361 gcgatctcct tcactcccgc atcgaatacc gctttcatga aggagttccc gtagtcgaag
    36421 aagtaggttc ccctttccac cagcgtcttg atcagggaaa aatgcttccg aaggctttcg
    36481 tcgaccctcc gccggtattc ttcccggtct tttgccagca tttcggtccg ctcttcgaag
    36541 gtgagtccct gcgggcagta gcctccctcg tagggggcat ggcacgaggt ctggtccgaa
    36601 agcaggtcga ccttccttcc ctggttcacg gcgtactcca gaaggtccac gatgttcccg
    36661 tgataggcga tggaaagcgg ttctttctcg gccatggctt tttccgccca gcggaaggcc
    36721 tcgtccaggt ccgcggtgac acgggaaacc catccctggc tgtgccgggt ttcgatccgg
    36781 gaggcgtcca cctccgcgaa aatccccacc ccccgggcga tttcaacggc tttcggctgg
    36841 gccccgctca tgccccccag gccggaggag atgaagaggc gcccggagag gtcctcgttc
    36901 ggtcccagcc ccagtttcag gcgtcccgcg ttcaggatag tgttgaacgt cccgtggacg
    36961 atcccctggg ggccgatgta catccatccc cccgcggtca tctggccgta gtttgacacc
    37021 cccagctgca tggcccggtg ccattcctcc tggttgtcga acatccccac catgaggccg
    37081 ttggtgatga tgacccgggg gctgtcgggg cgcgatcgga acaggcccag cgggtgtccc
    37141 gaggccacca cgagcgtctg ctcgtccgtc atgacctgga ggtatttcat gatgagccgg
    37201 tactgcatcc agttctggca gacctgcccc gtttcgccgt aggtgaccag ttcatacggg
    37261 tacaaggcaa cgtcgaaatc caggttgtta tcgatcatga cctggaaggc tctgccttcg
    37321 accgtctttc cctcgtactc gtcgatgggg cgcccccaga tcctcccctg aggccggaaa
    37381 cggtagccgt agatgcgccc ccttgtcaga agttcctgca tgaattcggg ggcgagccgt
    37441 tcatgccatt cttccgggac gtaccgaagg gcgtttttca gcgccagctc ggtttcccgg
    37501 cggttcaggc tgtatccccg gttcggggcg cgccggatcc cgggttcaaa aaccggatcc
    37561 ggcggaaaag gaccgtccag ccggatggtc atggccttgc tgatttcgct gttgatgctc
    37621 atgatctcat gcacctccag agggaacgcc cggttccctt tcgtgaatga ggggtgtgcg
    37681 gacacgcagg cacacccctc gcctcgaagg atccctttct acggacgaat cagttcgggg
    37741 tcgtccacca ctccgaaacg ccggaacaag ccgttcttca cgacctggac ctggactggc
    37801 tttacaactt ccccgatgtg ggtgaagccc ttgatgatcc cggtcaggcc atcaaaattg
    37861 gttgtcttgg aaatggccat ccggattgca gtggggttgg aatacgtctt ggttttcctc
    37921 atggcgtcta cgatgatcat gaacgcgtcg taggccgagg ctcccaccat gtccggttcg
    37981 atcttgtagc gcttccggta ttccttgagg aagttctgga caacgggccg cgggtcatcc
    38041 cggtcgaagt tggtaacgat gtagaatcct tccgctgctt caccggcgat ttcagccagt
    38101 ttcggagagt ccgccccttc ctctccaagg attttcgact tgattccaag ttcccgggcc
    38161 tgcttgagca acggtccggt ctggaaatag tagccgctgg caaagatcag gtcggggttg
    38221 gcttccttga tcttggagag gtagggcttg aaatccttct cgctgaaggg ataggcctgt
    38281 tcgtagacga tcttcatgtc cctgccgtat tttgcctggt acatcttgaa tccctcgacc
    38341 agggtccggc cgaaatcgtt gtcgctggtc aaaagcgcga ccgttttcgc gttcaggagc
    38401 ttgtgggcgg tataggctgc tgcttttcct tccaccgtgc ccaaaaaccc gttcctgaag
    38461 cagtactcgc ctgccttggt gacatccggg tggatggcgt aggcagcgac cagggggatc
    38521 ttctcctcct ggaaaatcgg ggcgacggca cgggtgggaa ggctatagga gcccgcgacg
    38581 aaagcagaga tcttatcctg ctcgatgagc ttgtaggcca gggcgaccgc ttctttggcg
    38641 tcggcccggt catcgtagac taccagctgg atcttctttc ccaggacccc tccctgcccg
    38701 ttgacctgat caacggcgat cttgacggaa ttcaaggcgc tggcgccatc cgccgccgca
    38761 aatccagtca aaggggccag catcccgatc ttgatcgtct ccgccgcgga agcaacgccg
    38821 atcaaaagac acaggatcac cagaacagcc gcaaaaaccc gaaaaccacg catttccgaa
    38881 acacctcccg aaatgaattt gaatcctgat tcgtgtgcct ggtctcaaag gcccaggtag
    38941 gcctctcgaa ttcgcgggtc atccttcagg accgcggact cgccctcaaa ctggatcctc
    39001 ccggtttcca ggacgtagga gcgcctggaa atctccagcg acgccagggc gttctgctcc
    39061 accagcagga tcgtcagccc cttctggcgg ttcagttcct gcaggacctt gaacacctcg
    39121 tccaccagct tcggcatgag ccccatggaa acctcgtcca ccagcaggag acggggatcg
    39181 gaaatcaggg cccttgcgat ggcgagctgc tgctgctccc ctccggagag cgtcgcggca
    39241 ttctgaccgc tcctctccgc caggatcgga aaaagggagt aaagccactc gagctgtttt
    39301 tcttcccggg ccctgtcgtg gatcccgtac aggccaaccc ggaggttttc cctcaccgtc
    39361 agccggggaa aaacacgggc cctttccggc accaggcgga ttcctgcccg ggcccgctca
    39421 tgggcggcca tcctcgagat ttccgccgcc ttgaagcgga ccgagccgga acgggcgggc
    39481 accagcccca tgatcgcgtt caggatcgag cttttccccg ccccgttcgc cccgatgatg
    39541 gaaaccagct cgccttcctc caccgagaag gaaaccccgt gtacggcctc gatgtccccg
    39601 taggcgatcc gcagatcccg gacctcaaga agcattggcg cttccccccc tccccaggta
    39661 ggcccggatg acctccgggt tgttccggat ggcttccggt tttccctcgg ccagtttcct
    39721 cccgtggtcg aggaccacca gacggtcgca caatcccatg gcgaccctca tgttgtgctc
    39781 gatgagaagg atcgagatcc cgctctcccg cgttttccgg accagttcct cctgctgggc
    39841 gatctcctcg tgccgaaggc cggagaagga ttcgtccaaa agcaggagat gcggtttcag
    39901 cgccagggct ctcgccagct ccagccgcct caggttcccc aggggcagga gaccggcttt
    39961 ctgccccgcc tggctttcaa gccccacccg ggccaggatt tccgacgctt cttcccgggt
    40021 cttgcgccct ttccaggaac ggcaggaggg gatcagtctc ccgtagcagg tcaggcccga
    40081 ggccacgatg acgttttcca ggaccgtcag gttcgggaag ggacggacga tctggaaggt
    40141 cctcccgatt cccagggcag ccatccggtg gggcggcaga ccgtccgtcc tcttcccgcg
    40201 gaaccggatt tccccggagg tcgggcggta aaccccggaa acggtgttga agcaggtggt
    40261 cttgcctgcc ccgttcgggc cgatcaatcc caggatctcc ccttcgtgca ggtcgaacga
    40321 cacccggtcc agggccttca ggcccccgaa gtgcatggag atctcccgca ggctgagaag
    40381 gggttcatcc atcaggactt ccctcccttc agccgcgaaa aggtccgacg ggccaggctg
    40441 ttctccccca acagcccccc gggctggaaa cggatcatca gcaggagaag ggccgcgtaa
    40501 aggaacatcc ggtagtccgt caggggcctg aacacctccg ggaggatgcc caggacgagg
    40561 gcccccagga ccgggcccca cagcgttccc atgcctccga ggacgaccat ggacaggatc
    40621 atgaccgaca tggggaagcc gaaatcggtg gcactgatga agcgcatgaa atgggcgtag
    40681 agggctccgc ccagacccgc catggccgtt cccaggatga aggcggtcag cttgaaccgc
    40741 acgggggaca cccccatgct ggacgcggcg gtctcgtcct cccggagggc gaagcaggcc
    40801 aggcccgacc agctccgggt gaacagccag cagatcgcca gggtggccgc gaggaagccc
    40861 gcgctcagga gaaagaagga tactccccgg atcctttccc cgaaaaggac gatccggggg
    40921 attcccccga tccccagggc cccgccgaaa aaggggacgt actggaaaat ggagacgacg
    40981 atgaagttga tcccgatggt ggtgatcgcc aggaagtctt ccctcaggcg gaggctggga
    41041 agccccagaa acagccccac gagaccggtg atccccagga cgaccggcag ggcggtccag
    41101 aaggatagcc cccccgccgt ggtcagaagg gccgaggcat aggccccgat cccgaagaag
    41161 gccgcgtgcc ccagcgagat ctgtcccgcg tagccgacga tgacgttcag gccgcaggcg
    41221 acgatggcct ggatggcgat caaggtcccg atcgtgatcg tatactcgct catgaaagcg
    41281 tcccctcccc tacccgcggc tgccgaggag cccctgcggc cgccagagaa gaacggcgat
    41341 catggcgatg aaggccaggg catcccgcgg gagagggatg cccgcgtagc cgatcagcag
    41401 ggtttccccc accccgagga gaatcgacgc caggaccgcc ccggggaccg accccagccc
    41461 ccccacgacg atcaaagcca gggtcttgta ggcaggcacc gcccccatgg agggatagac
    41521 ctggttgtag tagatcccca caaggatgcc ggcgatcgcc gccaccgccg agccgatcat
    41581 gaaggtcagg gagatgatcc ggtgactgtt gatccccatg gcgctcgcga tcgccatgtc
    41641 ctgcgaggtc gcccgcatgg caagccccag ctcggttctc gtcgtgatga accagagaag
    41701 ggcaagaacc ccggcgctga tgcagtaaac cgccgtcagc gtcgaggaaa tcacgatccc
    41761 ccccaccgtg aagctcggga acggcagccg ggccggaaac gagaggatgt aggggcccgc
    41821 cgcgagacgg cagatctcct cgatggcgag caggcaggcg atgctcccga tgagagggac
    41881 gtagggaggg tatttcagca gcgggtaata gacgaaccgc tcgatcagga accccaggcc
    41941 cgcacaggac accatggctc cggcggccga aaggagaatg ttttgggtcg cggcgaaaac
    42001 cgcaagaccg acataggctc caaccgtgta cacggctgca tgggccacgt ggaggatccg
    42061 caggatcccg tacaccaggg ccagccccag cgtgatcatc gcgtagatcg ccccgatggc
    42121 gatgccgttc agcatttgtt cgaggaaaag ctgcatggcg ttcgtcttcc tttggttttc
    42181 agttcaaccg gccgacggcg gcttctacct ccgccacgat ctttccggaa cgggtaatcc
    42241 ggtggacctc ccggatcagt ggctccatga aggcatcctt ttcatagaag gggaccgtct
    42301 cccgcaccgc cgcgtaggca gcctccgttc cctttcccgg cttcaggggc aggctgaaat
    42361 cgatgccctg ggccgccgtc aggatctcga tggcaaggac gttgaaggtg ttttccagga
    42421 ttgtcctggc cttccgggct gcatgggctc ccatggaaac gtggtcttcc tggcaggccg
    42481 aggtggtgat cgaatccacg gaggctgggt gcgccaggac cttgttctct gaaacaaggg
    42541 ccgcgcacgt gtactgcggg atcatgaatc cgctgttcag tccgctttct ggaacgagaa
    42601 aggggggcag tcccgagagt ttgtggtcca caagccgggc gacccttctc tctgaaatgt
    42661 ccgctagttc tgcgacggcg atgccgaaaa agtccatggc gagggcaatg ggctgcccat
    42721 ggaagttgcc tccgctgatt gcgatgccgt ctgtggggaa aatgatcggg ttgtccgtta
    42781 cggagttgat ctcaaggtcg atctttcccc ggacgaattc cagcgcatcg cggctagccc
    42841 cgtgcacctg ggggacgcag cgaagggaat aggcgtcctg gacgcggttt cccttgaagc
    42901 gctcaatgat ccggctatcc tggatcaggg tcctgatatt ctccgccacc agtttttgcc
    42961 cggtgtgggg ccgaagttcg tgggtcctgg gatcgaaggc gtagggaact ccgtgcagcg
    43021 cttcgaggga aagcgccgcg gcgatgtccg cgaccttgga gagcagcgat gcatcgtaga
    43081 cggcaagagc cgccaggccg gccataaagg cggttccgtt gttgagggcc aggccctcct
    43141 tgggtttcag ggaaacgggc ttcaggccgg tttcctggag agccctggct gtcggcatcc
    43201 tgcggccccc gtagaaaacc tccccttcac cgagaagggt gatcgccagg tgggaaagag
    43261 gacagagatc gccgctggca ccgacggagc cctggctcgg gatcaccggg tggattccca
    43321 aattgaggta attgacgagc tgggtcagag tctctaggct gattccggaa aagccccgcg
    43381 tcaacgtgtt gatcctcagc agcataatcg ccctaaccgc gtcttccggc aaaggttctc
    43441 ccactccgca ggcatggcta aggaggagat ttttctgaag cagttcactt tggtcgggcg
    43501 gaatcgaaac cgaagccagg tccccaaatc ccgtcgtaat cccgtagatt acctttccat
    43561 ccctggccca ttccctgacg atgcgccctc ccctctccac gaaggtcacc gctgcgggat
    43621 cgagggagac tccaaatcct tccctcgcga ccttgacgac gtcttcgagc gagagagagt
    43681 gtccgttcag tgcgatcacc tttccgtcca aaatagcccc tccttccgca gttttgaaac
    43741 cccgggtccg ggatattacc ggtgaacgac gaatccgttc tcctgcccgc aaaaccggca
    43801 ctttcccaat ggatctaatt caagctgcac aacattatat ccatcgcgta caacgatgtc
    43861 acgtccgcat ttcctgcacc gggtgattcc caggccgggc aagttgcccg ggtaaacgta
    43921 gaaaagcctg ccggacgcga tctgccggta ctcttccatg accggcaggg gggtagcggg
    43981 ttcggtccat ttccatcgtg ggaaatacct tgaaaggtgg agcggaatcg tttcgtccag
    44041 ccccgcgatc cactcgacca tgtcttcgaa ggaggccctt tcgaggttga ttcccggcac
    44101 gagcaggtgg gtgagttcaa catggacatt ggactttttc agcgtgcaga tcgtttccaa
    44161 aacgggctgg agatagcctc ccatctttcg gtaggccgtt tctgaaaaag ccttgagatc
    44221 gacgtttgcc gcggaaagaa acggaaggat cgcctccaga ggtcccggat tgatggtgcc
    44281 gttggtgaca agaacaacgt cgaacccttc cctttgaagg agcggggctg cttcggtgag
    44341 aaattcgtac caaacgagag gttcgttgta ggtgaaggcc acactcctgc ttcccgaatc
    44401 cctggccgct ttcaggaggc cctgaggcgt aatcctgaac agccccacgg agggatccca
    44461 gcgggccagc tcccaattct ggcagaaagg gcaggcgaga ttgcagccga cagtccctac
    44521 cgaaaggatc cggctgcccg gctgccagtg gaacagcggc ttcttttcga tggggtccag
    44581 gtggatggcg gccgccagcc gggcattcag ggcttcgagg gtttgtccgt cagcggaagc
    44641 ctgccgcact ccgcagcgcc cccgctgccc cggttgaatc aggcaacggt gcgggcaaag
    44701 ggtgcagcgg acactgccgt tttcccggtt ccaccagagc gcccttgcgg cccccgcctc
    44761 agacccgttc attgttcttc ctgctcccga aaccgctcga ccgtaaaccg ccacaggtcg
    44821 gctccttccg ggccttgaag ccctgccttg cggctggcga tgaaaagctg ctcgtcgacg
    44881 gtgtcgaccc cttccaggtc aggcaaaagc agcccgcgcc gattcccctt ttcgacgatg
    44941 acgccaaacc gtttcggatc gagcgattcg ggccccgaaa cccgttcagg tcgcccaagc
    45001 acgtcaaccg agaaaaccag gtcctgcaat tctgccaggc tgacgggttc gaagcgtgga
    45061 tcctgggtgg cgccggctat cgcgtttcga atgatttctt cgcccagcga ggggcatgtc
    45121 ggctcgatcg tgccgatgca gccccgaagg ttgccgttcc gccgttcttt cagggaaacg
    45181 aagcaggctt tcctttcggt ccagattttc ggatcgggca agatttgccg agcctgttcc
    45241 acgggaagcc ttttcccggt cttcaggtaa aagagcactg cctcccgggc aagatgcggc
    45301 agtcccttcc tttccgcctt ttcccagaac gcgattccgt accccacccc gaacggggct
    45361 tcgtaggaga ggacctcgat cggtccgctt ccggcgagnn nnnnnnnnnn nnnnnnnnnn
    45421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    45481 nnnnnnnnnn nnnnnnnnga gctttttccc cagactgtag gcggcgtcga ggtccaggcc
    45541 gatcgggttg gccaaaacga gaggcggcag ctgtccccag gcttcggcaa accaggtcag
    45601 gggaacgacc gaggcatgat caagggcgaa cttggaggtc ctccaggtgc ggagacgaat
    45661 cgatcccccg aggaaagcgg acagtttctg gacggcttcg gggtttcccg gtgccgtaaa
    45721 gaaagaaccg ggaacaccga attgctgaag ccccccctcg aaggtgtctg catcgacaaa
    45781 catcagtccg tttccgaacg gtgcgtgagg actcaggacg aagagcagat cgggtttttt
    45841 gcacttcacc ttttccgcaa ggattttcat cccctggagg gttttcgacg cttctcgctc
    45901 cctcccccgg cctaccgccg ggacgatgat gggcggatgt ggagccaaac aaccccaaag
    45961 ccacatggtt tccccccccc ttctctcctc aatcaatata ctccaaccct cccattttgt
    46021 tgaggaatgc ggggatccgg aaggccactc tattcgatga acatcgcgtc tccaaaagag
    46081 aaaaagcggt acttttcccg tacggcggtt tgatacgcct tcatcatggg ttcatgcccg
    46141 gcaaacgcgc taaccagcat caggaggctg cttcggggca ggtgaaagtt cgtgatgaga
    46201 gcgccgatcg ccctgaaacg gtatccagga tagatgaaca gccttgtccg attgatgccg
    46261 ggcaggcagg ttccggtttg ggaagaagcc tccagggccc tgacgacggt cgttcccacc
    46321 gcaacgattc tccttccccg gcggagggct tcgttcacgc tttgggcagt ttcttcggga
    46381 atttcgaaga attcttcgtg cattggatgg ttgcggatat cagactcttt gaccggtcga
    46441 aaagtgccga ttcccacgtg aagggtcagt ctacggatct cgatccccct ggaacggatg
    46501 ttttccagaa ggctttccgt gaaatggagc ccggcagtgg gagcagccac cgcgccttcc
    46561 cggcttgcgt agacggtttg gtagcgttcg ggatcgatcg tgtcgttctc gatgtacggg
    46621 ggtaagggaa cttgacccgc tttggcgatc aagtcatgga cgtcgagggc ttcgggaaac
    46681 tgaacccgtc tcaggccctc gggaagtctt tcctcgatcc gcacggtgga tccttccgcc
    46741 agaaggaccg tcgttcccgg ttgaagtctc cttccaggtc gaaccagcgc ttcccactct
    46801 ctccagtcgg ggtgtgtcgg ccgcaacagg aagatttcgc atcgggcgga cccgcccatt
    46861 ttccgcccta gaaggcgggc ggggatcacc cgggcgtcat tcagcacgag aaggtcgtct
    46921 gggcggagga actctggaat ttcccggaaa acccggtgct ccagcaggcc tgttgaacgc
    46981 ttcagcacga ggagacggga cgaatcgcgc ggggtcgccg gcacttgggc gatttgctcc
    47041 gggggaaggt cgtaatcgta ggcatcgatc ttgagcagat ccggcagggt gtctttcggc
    47101 atcccgcctt cgaggtcttt cccgttcatg aggctggccc ttttctctca gaggatcgcc
    47161 tgaagcgttg tacccgggaa gtaatgccct agaatggcct cgaacttcca tccgttttcc
    47221 gccagggctt ttgcacccca ttgggagagg ccgactccgt gtccccaccc cttcccctgg
    47281 aattcaaaag cggggggaac cgtcccggga aacggggctg agggtgcagg agccggaagg
    47341 gggaccgagg ctttcgtttc ccctttcgac agggcctttt tgaggtaatc ggtttttttc
    47401 tccgggtgaa gaagcatgtc catcagctcg tcgctggtga acgccccctg tctcgtcaga
    47461 acgacgaggg ggtcctctcc cggggctccc ggaggcgccg ctggtcccgg gacgggtttt
    47521 tcgggcggat tgaaaaacgt tgagccgtaa gccggagaga cttcgaaaag ggtgcttcgg
    47581 atcaggttgc ttcccgctgc catcctgaag gaatgtccct tgatggttgc gttcccggaa
    47641 gttcctctca cttcgaggag agtcacgcgg ttcccggcgt ccctttggag cgcccggatt
    47701 tccaggatgg atccaacggg ctgccggaga gtgttcatga tcttctgaag ggcttcctgt
    47761 gtcagggagg ctctccagcg agaatgaggg gtttcatagg gaaccggttc gggacggcag
    47821 gtcagatagg gagttttccc gccccagacg ctggaaacgt cggctgtcca gccggctccg
    47881 tccgcaaaat aataggtctg ggcaagttcc gagccgaaaa tcacgacgat tccccgggtt
    47941 tccgcgatgg cccggtcaag tttgggatct tcagcggcca ttcccctgta aacctggcaa
    48001 tgtgtcgtgg cgcagaggtc aaatccttcc gctccgtggc ggcccgcctg gcgcagggca
    48061 taggtgcggg caatgacggc ctgtgccttg agggcttcca ttggccatgc ggggttcacc
    48121 tcgatcttga ggacaccacg aaggtaggat tccacatcga ggacgttgac gaccgaaaag
    48181 ccattttggc gggaaaccag tcggagcctt ccccggtaag gtttcccgtt ccagtagagg
    48241 aacccctggg aactctcgaa ctcagccggg agtgaaacct gcttcccgtt gacaaggatc
    48301 cctccgggaa cggattccac gctagccgtg gctcctgcgg agaaagaacc gcctgaggca
    48361 accaaaacgc gaatggcgga gttcgaatcg atccgtcccg agaagacccc tgtttccaga
    48421 ccgacacgga cctgccggga agaggcctcc aggtgcgagg aagggagaag gaaaaaaaga
    48481 gcgattgcga ccggcaggag aacggaaaag aaatggatcg gctttttcat ttgaaccacc
    48541 tcaggaacag gttcaacagg atcgtcagga cgaggctgag gagaagcatg ctcgcgatgg
    48601 gcgcgaagag aatgaagtgc ttgccacgga aggaaaggtc tcccggcaac cgcccgaaag
    48661 gcactcccgc ttttcccgca agcagcagga ggccccccag cagaaccaac agcagtccaa
    48721 tctgaacgag ggttttccca aacgtgtaga ggttcattcg gctccctcca gaaggggcat
    48781 ctgacgtgca taccccgcgg gaggcttgat cccgaggtat tcgaaggcgg caggggtggc
    48841 ccttctgccc cgtggcgttc gttcaatcag tccctttcgg atcaggaaag gttcgtaaat
    48901 gtcctccacc gtctgttgct cctcgttcag cgccgcggcc agggttgcca ggcctaccgg
    48961 tcccccggaa aacagctcga tgagtgcccg tagcagtttc ctgtcccctt cgtccaggcc
    49021 ggaatgatcg atcgatagca tttcaagagc ctccttggcg aggctggcgt cgatcctggc
    49081 atggttcgtg accgaggcga cgtcccgcac ccgcttaagg aggcgaatcg ccacccgggg
    49141 agtgcccctt gaccgctggg caatcacgcc cgcggccgaa gggtcgatat cgattttcaa
    49201 aagggcagcc cctcgcatga cgatttccga gagttcctcc gcggtgtaca ggtcgagctg
    49261 ctcgacgatc ccgaaacggg cccgcagcgg ggaagtcaaa agcccgagac gggtggtcgc
    49321 cccgatcagg gtgaaggcgg gcagtttcaa gcggatgctt ctggcgaaag ggcccttgcc
    49381 cacgatgatg tccagcgaaa aatcttccat ggaggggtaa aggatctcct ccacgttggg
    49441 agaaaggcgg tggatctcat cgataaaaag cacgtcgccc gcctggatgt tggacaggag
    49501 ggcggccaga tcccctgccc tttcaagcgc tggccccgtt gtgaccttca agttccccga
    49561 catttcgttc gcgatgatcc cggctagagt ggttttcccc aagcccgggg gcccgaagaa
    49621 caggaggtgg tcgaggggtt cctttctcat tcgggccgcg tcgatataga tccggagttt
    49681 ctctctcagg gatttctgtc cgacgaattc ttccaggctt tggggtctta gcgaaaggag
    49741 atcggtttct ccgacccccc ggaaaggttc gaccattcgg ttcgtctcct gattcatcga
    49801 tactcacccc tgatgcggtt caaaaggcgc aaggcggatt ccaggatctc ctcttctcgg
    49861 gctccgctat cggcttgcga aagcgcggtg cggacggcgg cagcggcttc atgcctggag
    49921 aagccaagcc ccaccagcgc ctcgagcact tcgctacctg ccggcgagga tgccggttcc
    49981 gccccatcgc gggtgaatga agggaagctt ttcgcgagtc tttcccgcag ttcgaagcaa
    50041 agccgttcgg cggttttctt tccgacaccg gggactcttg tcagcgtttc caggtcggag
    50101 gaaaggatcg cccggatcag ccttccgggg ggttccgccc gcagcaggct catggccatg
    50161 cggccgccga ttcctttcac ggcaagaagt tcgaggaaaa gccgcctttc ctcgtcgttg
    50221 gcgaagccaa agagggagat ccccgattcg ttgaccaaaa ggaaggtctg aatgcgggtc
    50281 atctcccctt cgacgcagga ttcgagcgct ctccggctgc aatggacctc gattcccagt
    50341 ccgttgacgt cgaggaccac cgtttcgtcc tcgacctgaa tcacctgtcc gaaaagactt
    50401 ctcagcatgg ggccggtcct cctgcaggat tcagcctgga ctggacggac cgaatggcaa
    50461 ggcccgtcag ggcgatcgca agcgcatcgg aaacgtcgtc cggtcgcgga ggcgccggaa
    50521 gacgcagaat ctcacaaacc atgtgctgga cctgcgcctt tgttgcgttg ccgttcccgg
    50581 caaccgccat tttcacttcc gcgggcttgg gttggaaaac ctccagaccg cgctgagcgg
    50641 ccagcagaag aacgacaccc cttgcttgcc agacggcctc tgcggtcgtc gtgtttctcc
    50701 cgaaatacag gcgttccacc gctaggaaat cgggagaaac acggtggaaa atctcctcaa
    50761 gcgaggaaaa aaggcagagc aggcgcttct ggagaagatc cccgggaggg gtttcgatgc
    50821 agccatattc aacggccgtc agggtacttc cagcctgggc gacgagcccg aagcccatcc
    50881 tcccgatccc cgggtcgatc cccaggcagc ggatggagcg gcgatcagcc agcaatagcc
    50941 tccatcacct catcaggaat gtcgaagttg ccgtagacgt tctggacatc atcgtggtct
    51001 tccaggattt ccatcaattt caggactttc gcggcgtctt ccttcttcgt gatctccacg
    51061 gaattcttgg ggaccatgct gacctccgcg tttgcaacgg catagccttt tgccgcgagg
    51121 gcttccttca cctgggacag gacggaagga tcacagtaaa ccgtgtagcc atcctcgccg
    51181 tccggcacca ggtcatctgc cccgccctcg atggaggctt ccatcagctt ttcctcatcc
    51241 agccccttcc cggtgacgct gatgactccc cgcctttcaa acatccagct cactgagccg
    51301 gcctccccca tgtttccgcc gtttcgggaa aaaagggatc gcatttccgc gctggtccgg
    51361 ttgcgattgt ccgtcagggt ttcaacgagg atcgccacac ccgcggggcc gaagccctca
    51421 taggtgattt cctcgtaggt cgctccttcg atctctcccg tcccccgctt gatcgcccgc
    51481 tcgatattct ccattggcat gttgtactcg cgggcttttt cgatggcggt tttcaggcgg
    51541 aaattcgcgt tggggtcacc gccgccttca cgggcggcaa cgatgatgaa tttggcgagc
    51601 ttctggaaaa tcgctccctt tttcgcgtcc tgcgctcctt tccggtgctt gatgtttgcc
    51661 cactttgaat gtcctgacat cgatgatcac ctcttcatca agcttgtttc gtctcgctga
    51721 ttcggcaaat gggaggagtt tgcctcttgt gctcggaacg ggcacgggat tgttccacga
    51781 agctgcgaac cctgtccgat ccttctccca gcgccaggaa acggtccagt tcttcatagg
    51841 tgacgcccat ttccccctcg tccgtctgcc cttgccagag ccccgccgaa ggcggctttt
    51901 cgataatggg cgcgggaatt tccaggaggc tggccaattg ccggacttcc ccctttaaga
    51961 gatcccccag cggcagtagg tcaaccccgg aatccccgta ttttgtaaag tagccgacgg
    52021 ttagctccac cctgtttccc gttccgcaga ccaggaggtt tctggcctgg gcaaggaagt
    52081 agagggtggt catccgcagc ctgggtttca ggttggccct ggccagccgg ttttcttcgc
    52141 ctgcgtcctg cccgagagcc gaaatgagag catcataggt ttgggtcagg tcgacggtca
    52201 tggtggggat ctggagatga acagccacca tttccgcgtg aaggcgatct tcggggtggc
    52261 tgtggcaggg catgataacg gcaagcatgc ggttcccgca gacgcgcttc agaatcgcgg
    52321 agaccacggc ggaatcgatg ccgccgctca atccgacgat tccgcccttg cccccagcgg
    52381 agcgaagctg ttctgcaagc cagttctcga tcgaagaaag caggattgac gggtcgcggt
    52441 aaaaggtggg catctctagc caatctcccg atcttgaaag ttaaaaccag acacattcta
    52501 gcatatccgc tgctcttgct tcaggccttt tcagctcatc gaagaaagca gggaatacag
    52561 gtcccgccgt ctcccttcca gaagcgggag ctttttcctg acccgctcgg ttgccgccgg
    52621 atccatttcg aaaacaagcg tttccggccc cttcccggct tctgccagga gggttccgtc
    52681 aggggcgatt cccatggaat gcccgcaacg gttgcaggcg acagtgaaga tctggttctc
    52741 gatcgagcgg gcctgcagaa gggcccgcca ttgagccatc tggttttccg gccaggatgc
    52801 gcaaagcagg agaattcgac tcccggcgag tgcaagggtg cggatgaatt cggggaacag
    52861 gaggtcccgg ccgatcgcga ttcccaaaac gcttcctttc cagaaaaagg gtgaagtcgc
    52921 cttgcccggc cggaaaaagg aatcctcctc aaccgaggga aggaggtggg tctggtcata
    52981 atgggcgatg acccttccct cctcgttaag gaaaaaagat cggttgaaga ggaacccgtg
    53041 ttcaacccag ggaagactcc cgcagtggat ggcgcagaac ctccggcttg cctcatccga
    53101 cagtcgctcg aggagggacg ggctgaatcg ggcgttttcc ggcagcgctt cgtcaacgtg
    53161 cggcacgctc cataattctg gtaagatgac gagcgagacg tcggcaggaa gctcccgaag
    53221 aaaagaatcc acctgaaaga cacgctcaag cggcgaatcc gggccacttc ccgtctgaat
    53281 gatggcgaca cgaacaggag gcatcctgtc tttccccctt ccgaggcaat ggaggtgagt
    53341 cgatttgacc gaaaaactgg ttcgtttcag tgtttccatg ccggaagcgc tggtcagcga
    53401 gttcgaccgc tggatcgaaa aaaagaagtt gaaaagccgt tcggatgcct tgaggcagat
    53461 catccgggac tacatcgggc aaagcctctg ggaagtccag gaaggcgaag cctacgggac
    53521 cttcaccttt tcctatgacc accacagccg cgacgccagc agcgaaatca cacgtctcca
    53581 acacgatttc atggagtcca tcgtttgcac gacgcacgtc cacatcgacc atgatacgtg
    53641 catggaggtc gttgtgctac gaggctcgac ccaggaaatt gtccgcttcg tggaagcggc
    53701 ctcttccctt ggctgcatcc gggttggtgt ccccggcctg ttgccgccga tctaacggct
    53761 caaaaagggg cctcccctga aaggaggccc cttttccttc gtcagaaaga acagcgacgg
    53821 ctagtttgcc agtgtcgcga ccatgaccgc cttgatcgtg tggaggcggt tttcggcctc
    53881 atcgaaaacc ttcgaatttt tggattcgaa aacctcttcg gtcacttctt ttcccttgac
    53941 ggcgggcagg caatggagga agatcgtgtt ctcctttccc gtggcggcca tcagcttcga
    54001 gttcacctgg tagggggaaa gcagggccac ccgttccgcc atcttgtcct cctctcccat
    54061 cgacgcccag acatccgtgt aaatggcatc ggcacccttg acggcttcca tggcatcttc
    54121 catgaactgg atcgttgccc cggtttcttt tgcgattgcc tggcacgttt cgaccaaatc
    54181 ggattccggc cagagggact tcggagcggc gatcacgaaa tggagcccca tctttgcgga
    54241 tccgatcatc agggagttcg ccatgttgtt gcggccatca ccgcagtaca caagccggat
    54301 ccctttcagg tggccgaagt tctcctggat tgtcaggaaa tcggcaagaa tctgggtggg
    54361 gtgataggta tccgtcagcc cgttccagac cggcacaccg ctgtaacggg ccagctcttc
    54421 gaccacgctc tgcttgaagc cgcggaactc gatcccgtcg aacatgcggc caaggacccg
    54481 cgcggtgtcc ttcacgtcct ctttgtggcc aagctggatg tcgttcttcc caaggtattc
    54541 cgggtgagcc ccttcatcga tgcaggcgac ggtgaaggcg cagcgggtcc gggtcgaggg
    54601 cttttcgaag atcagggcga tgttctttcc ggcaagcagg ttcccccgga tccccatccg
    54661 cttttttgtt ttcaggtcgg cggacagttt cagaaggtat tcaatctcct caggagtgaa
    54721 atccttgagg gtgagaaaac tgcgaccttt caggttaact ggcatgtgac tcttcctcct
    54781 tttgattgtg ataaaccgta aaattgattt ttccgaaacg ggagtcagcg ctttggttcc
    54841 cgttcgagag gcatgctcat gcagcgagga ccgcctcttc cccgccccag ttccgccccc
    54901 cgaatctcgt agaccttgat cccgttttcg cgcaaaaccg tgttcgacac aacattcctc
    54961 cggtaggtca cgacgactcc cggagcgatg ctaaaggtgt tcgtgccgtc attccactga
    55021 tccctggcag ccaggaccgg gtctccgccg ccggtttcaa tcaggcggac ctggtccaaa
    55081 tggagagatt tcttgagaac ctccacaagg ttcaggcttt catcgatccg aacgaggttt
    55141 ccgccctcgt cgtaggaaag ctcccagaca cggatgcggt tggcgactcc agggaaaatc
    55201 gtgaaggcgt cccagtcgac catggtgaaa accgtgtcca ggtgcatgta agccctgtgc
    55261 ttggggatat cgacggcaag aacggttcga atgccggctt ccttggccag cctgcgcccg
    55321 acgatctgga ccgatcctgc ggtcgttctc tggctgatgc caatggcgac cgtatcttcg
    55381 gaaagaacgt ggaaatcgcc tccctcgatg cagtaaggca cgccgtcgag tttttcttcg
    55441 ccgaaaaaga ttttcacgtc cttgaaatag gggtgatact tgaagatgta gcgagcgtag
    55501 atcggttcac gctgtcttgc gctgaagctc atgacgctga tgatgacgcc gtttttgacg
    55561 aaggaatagg gatctctctg gaagtagagg ttgggcaaag gccggatcaa aagctcgtga
    55621 tccgccagag tgttcagaac aaggctctgg cagtttccca agagttcgcg ggcttccgct
    55681 ttcgtcagcc cgccggtcag gatttccgca agttcggggg cagggacgct catcatttct
    55741 gttttcagac cgcacaggag gggataatca agcgcctccc acgccaggac ctcgtcaagg
    55801 agcatggagc ggatctcttc ttcccgcagg acgtgggcca ggcaatttct gaaatagacc
    55861 acctcgacgc cgttgtcgcg gaggaggtcc gcgtacgcgt cgtgctcctt ctgggcctct
    55921 tcaacccaca ggatgtcgtc gaagaggagc tcgtccttgt tgtcgatcgt aaggcgttct
    55981 agttccttgc cgggccgatg gagcatcacg cgcttgaggc gccccacctc ggaagacacg
    56041 ttgaaaccgc ttgtcccttt gtccatgagc ccctccttaa aaatgaaccc gaacatgtct
    56101 aaggttcagg ataggacatt tatatcacaa agggatgggg cgaaaaaggg ggggttaccc
    56161 ccactggagc aggatttccc gaacgcattt tgccgcaagg gcggacgtga cgttgttcac
    56221 atccaggtcc ggggccactt caacgagatc gaagccgaca aggttgagac ctttcatggt
    56281 gcggattgcc tcgaggagtt cccgtgaggg aattcccccg gcttccggtg ttcccgttcc
    56341 cggggccgcg gccggatcca ccacgtcgat gtcgatggat aggtagacgg ggaacggttt
    56401 cagttcatcc acgcgacgac tcaagggttc aaggacgtcg tccaggtgga ggcgggtgtg
    56461 agccttcccc caggccatct cctccttgca cccggagcgg atcccgaact ggtagaggcg
    56521 gtcgggttca ccaagacaag actgcgcaac gtgccgcatc accgaggagt gactcagcgt
    56581 tccgcctctc aggcttgtcc tcaggtctgc atgggcgtca aaatggacga cccgaagttc
    56641 cggttcccgt gacgcggcgg cccggatgag gggcaaggaa atgagatgtt cgccccccag
    56701 gctcccgatc cgctttcccc gtgcgatcaa acgttgtgcc acccgctcgg tggtatccag
    56761 tgcttgatgg acatccccgt agggaaaatg gagatcttcc agatcgaaaa aggtcacttc
    56821 cgaaaggtcc cgatcaaggg ccgggctgta ggtttccagg ttgtaggatt cggcccggat
    56881 ggactccggg ccgaatcggc tccccgactt acgggaaacg gtgaaatcca tcgggatccc
    56941 gagaaggacc catttcgacg cctgaaggtt tctcgagctt ccgataaaac gcgtcacgcc
    57001 tggttcggtc cttcctccag gattttttcc aaaaacgggg gtaacgtgaa acaggcctca
    57061 tggattttcg aagagtagta ccgagtccct tccggcggct cgcggaagat cttccgggga
    57121 tcgtggacct tggaggcgaa agtgtaggtc caggaacccg tgggataggt cggcatgatt
    57181 ccccagccaa gctcgacgat ggggaaaacc ttcttcatct ccaggtaggc agtcctgagg
    57241 atgtcaggat ggcccatggg agattcggtc tgtgaaacca ccatcccatc ctttttcagg
    57301 gcgcggtgga cgtcccggaa aaaagcctct tcgaaaagcc ctgctgcgaa gtccaccgga
    57361 tcggtgctgt ccacgataat gacgtcaaac tcgtcctggt gatccttgat gtagaccagg
    57421 gcatccatgg gccggatttc gactttcgga tggtccaggg cacagctcac cgaggggaaa
    57481 aaccgccggg acgcttcgat gaccttctca tcgatgtcga ccagggtaac tttttccagg
    57541 atgtcgtaac ggaccagttc ccggacgatt cccccatccc ctcccccaac gaccagcacg
    57601 cgccgggggg aaggatggga gcaaagggcg acatgactca tcatctcatg ataggtgaac
    57661 tcgtcctttt ccgtgatcat gatggcgccg tcaagagcca tgaggttgcc gtactgttcc
    57721 gtcttgacga caaggaggtg ctggtagggg gtctggatgt taaggaggat ctcgctgatc
    57781 cgcagcccca gcctcatgtc tggggtctgg tactcggtga accagagatc cgtttcccgg
    57841 cgggccatcg gctccatttc tctttattct cccttcttcc ggtagaaaag ggggcaaccg
    57901 gcgaacacgc tgccacactg ctccacaacg tgatcgactg ccttgacttt cacttccctg
    57961 agggcaaggg cgcgcatttc aaacgcttcc cgcaccattt cctcgacttt ccgggctgct
    58021 tcgtcggccg agcagaaccc cgaatattcc atgatcatgc cgaaactgcc gggatcttcc
    58081 ggtacgccca ctccgattgc ggcggcgatc cgctcaccgg gaacgtcgct cgaaatggag
    58141 ccatatgcga tggggaggag gctaccgtag ggcatctcca ttttctgtgc gaactcgcag
    58201 cggggcggaa ggacactgga aacccggaga aggttcatat taccgacacc ggcttccagc
    58261 agggctgcgt cgaatgcggt cagttttttc tccccttcgc cttttcccac gacaagggtg
    58321 tacagatctg gtgtttcgat catctttgga gtcctccttg gttgcgaatg gtcccgaagg
    58381 gatcgggaca agggcgaaat ccattatatc ggacccccct cccctgtcaa ttgaatggcg
    58441 gaaaaaaaga aagcgggagt tcaaaaaaat gggatttgtt taggggaaat gcgggggaaa
    58501 aggccattca gttttgcttt ggacagattt tttccagtgt ttcccggaga atgtcttcga
    58561 gattgccggc ttctgcgaca gcgatccgcc ttccatgcga aaacaggata aagccgcccg
    58621 cggagcctga aatgcccagg tcagcatggg ccgcctctcg gggcccgttg acctcgcagc
    58681 ccatcacggc gacgctgaag ccgtcaggta gaccggaaag agacggaagg atcctctgga
    58741 ccagggcctg cacatcgatc cgctttcgcc cacaggtcgg gcaggaaatc aggtcgaatc
    58801 ccttccttcg gatgccgaga gcgttcagga tcgaataacc ggttttgacc tcctcttcgg
    58861 caggagcggt gagactgacg cggattgtat cgccgattcc ctggttcagc aggatgccaa
    58921 tgccggccgc gttcttgacg attccgccga gtcccgggcc tgcctcggtg atcccgatgt
    58981 gaaggggata ggggtagcgt tcggcgagaa gcgagttcgc acgcactgtt tcctggatgc
    59041 aggtcgattt tgccgaaagg atcacatcgt cgaactcatg ctcgagaagg tatttcaact
    59101 gggcttcaac cgcccgaacg agagccagcg accgatctcc accggattcc gcgatctgcg
    59161 cgtccgacag ggagcctcca ttggccccga ttcggatcac ggcccttcgt tccctagcga
    59221 ggtgaaggat ttcggccagg cgtctgggac ttcccatgtt tcccgggttg atccggattg
    59281 ccggtacccc ggccttcagg ctcagaaccg caaggtcggg gttgaaatgg atatccgcca
    59341 tgagcgggat ttccgcctcc gaaacaagct ccgcgagagc ttctgccctt gaagggtcgg
    59401 aaaatgcgac ccggaccatc tcgcaccccg cggaagccaa cccctgaagt tcctcccggc
    59461 aagccttcag gtcgtgcagg ggggatttca gcatgctttc cacccgaacg gggttgcctc
    59521 caccgattcc aagacatcca acggaaacgc ttttcacgga catggggccg cggcctctct
    59581 ctttgctccc ggcctcaggg gtgggtcagc cggagtatgt ccttccaggt gacatacacg
    59641 atcagggcca gaagcagcgc gaaaccggtc aggtgaatga ggttctccca cttgggcgga
    59701 aggcgtctcc cggtcaggat ctctccgatc agaaacgaaa gtctcccgcc gtccagggcg
    59761 gggaagggga acaggttcag aattccgagg ttcaagttga tcaccgcgag gaacgagagg
    59821 aaggaccacc agccctgtct ggcggcatcc cccgccatgg aggcgatccc gacgggtccg
    59881 gcgaactcca cgctctggtg gccgaaagcc caagtccaga gtcctcgaag gatggacagg
    59941 gtcatttccc aggccgttcc gacagagcgg aaaagcgctt ccggcaacgg gtagcggatc
    60001 tgtctcggcc ggatcccgag cagcgggatg ccggactgct tgtcgatttc cgccttgacc
    60061 gagaacgtca ggatccttcc ctcgcgttcg actttcaggg acaaggggcc tttcggggct
    60121 tcacggcgga tggttgcgga catcgagtcc cagttcgtca cgggtttgtc gttgatcgaa
    60181 acgatccggt cccccgaaac cagtccgatt cgttccgcgg gaaagccgct gatggtcgat
    60241 cccacggtgg cggtccggat atcgagaacc ccatggaacg tcaaaaggac cgtcgtcagg
    60301 atgatggcca ggaggatgtt gaaaaacggg cctccggcca gaatggcaaa gcgctggaaa
    60361 ggggttttgc cggggtaagt cctttcggga ggggcgctct ccccttcctg ctcctcttcc
    60421 atcccagcca gccggacaaa gccgccgacc gggaaaaccc gaatcgagta cagggtttca
    60481 ccctgccttt tctggaaaag ggatgggccc atcccaaagg cgaactcgta caccttcacc
    60541 ccgtaccgga tggaaaaaag gaagtgaccc agctcatgga taacgacact gatgcagatc
    60601 acgagcagaa acgaaaatag gctgacgatc aaaaggtgga cctccttgat agttcgctct
    60661 cgccaagaat ttcagcggct tttctcctgg cccattccat tatagcgatt gcctcttcgg
    60721 gggattgcgg ggatggctcg tcgaaagcgg caaggacttc ctcgatcagc cgggagattc
    60781 ccaaaaacgg gatcttctcc cgtaggaagg ctgaaacggc gatttcgtcc gctccgacca
    60841 ggatagccgg ggcggcccct ccgcttcgtg cggcctcgat ggctagagca aggcagcgaa
    60901 aacgccgagg gtccggcttt tcgaaggaaa ggaccaagct ttcaagcggg gcaggatcca
    60961 ggtgcgcccc ttcgagttcg actctatccg ggtatcccag gcagaccgct gccgggatcc
    61021 gcatattcgg gttcgacagg agccatttca tggagccgtc gcaaaaatgg accatcccgt
    61081 ggacgaagga ctgcggatga accagcgctc ggatcctttc ggcaggaagt ccgaaaagat
    61141 gcatggcttc aaggatctcc agccccttgt tcatcagggt tgcactgtcg acggtgatct
    61201 ttgttcccat gtcccagatg ggatggcgga gagccatggc cggcgtcact ttttcaagcg
    61261 atgcaaggtc caggttgcgg aaaggcccgc ctgaagccgt cagcacgatc tcacggaccg
    61321 agcctttgcg ttctcccgcc aggcattgcc agacagcgtt gtgctcactg tccaggggcc
    61381 gaagctggtc ccttgcctgg accaggggca tcacccaggg gcccgcgatg acaaggcttt
    61441 ccttgttcgc gagggaaacg gtcttcccgc atttcaggga ggacatgagt gcggggatgg
    61501 ccgaggttcc cgaagaagca aagacggcgt gtcccgtttc gggtaaaagg acgaggtcct
    61561 ccagggattt cgggccggaa aggactttcg tcgtcacggg cacccgcaag gcaagttccc
    61621 gggctgcatc cggatctgac atggccaaga agggcgggtt gaactccgcg gcaagttcgg
    61681 ccatccgcga aacgttgcgg gaggccgcca gggcgaccac agggaagcgg tcaggatggg
    61741 cccggcaaac gtcaaggacg gccgttccga cgcttccggt tgcgccgatc accgcgattc
    61801 cggggggagg ccccgcttga ggtctcaatg ccaaatcacc tcaaagatca gaaaggcaag
    61861 agttgcattg atgaggatgc tgtcaaaccg gtcgagcatt ccgccatgcc ccgggatcag
    61921 ctttccggcg tccttgaccc ccgcttcccg tttcaggagc gattccccaa gatccccgat
    61981 ctgtcccgca agcccgcaga ggagtcccag caggaggagt ggaaaaggcg ggaactgcca
    62041 ggcaaacgcc agcaggccgg cgcagaacag gcttcctgcg acgccggccg cgaatccctc
    62101 ccaggtcttt ttcgggctga cgttttcgca gagataggtc ctgccccagc gaactcccac
    62161 gaggtaggcc gtcacatcgc agctccaggt gcacgcgaag agggtgatga gaagcatggt
    62221 tccccatggt tctgacctta gaaggacgag aaatgtccag ggaagcgcga tgaagatcag
    62281 tccgaccaga gttcctccca tgttcgggat ggcgtggctg ttctggagaa cctggcggcg
    62341 aacgatttca atgcaaagga cgagaaaggg gatcagggcg gtaatcgcca gaatcgccgc
    62401 agaactgaaa ttcatcgttg cggccgcgat gagcagaaag gtcgaaatga agccgagtcc
    62461 acgggtgact ttaaccttca cggaaagcag gttgtaaaac tcccaaaggg aaatcatcgc
    62521 ggccaacgtc acgatgaaag accagtagat ccccccaagg aggatcccgc caagaatggc
    62581 caggaccaca aaggccccgc ttagcgcccg tatgatgatc tcactgccga atgtccggct
    62641 agacggctcc atatcgtctc tccctcccag agtaggcttc cagggcctgt gcgagatcct
    62701 cgggaccgaa atcgggccag agcttgtcgg taaagtaaaa ctcgctgtaa gcgctttgcc
    62761 atagccagaa gttgctcagc cgcatttcac cgctcgtgcg gatgatcagg tccggatccg
    62821 ggatatccgg aagataaagg tacttcctga gcgtaacctc gtccagaggg aaaggttctc
    62881 ccgcggcagc cgcccggttc atggcgtcga ggagttcctg cctcccgccg taattgaggc
    62941 agatgaccag ctgaaggcct ttctgccccg cggttttctc ctccgcgtcg cggacgatct
    63001 gctggatatc cgcaggaagt ccgtcgattt ttcccgcgaa ccggatcctg gccccttcag
    63061 ccatcagggt gccaatcttg ctccggatat agattcggaa aagccccatc agtcctgaaa
    63121 cttcattttc gggacgtttc cagttttcgc ttgaaaaggc ataaagagaa agcgtccgga
    63181 tccccaggtc attcgccgcc cgaacggtcc gttccacagc ccggacccct gcgtggtgtc
    63241 caacaagacg ggggaaccct tttcttttcg cccatcgtcc attcccatcc atgatgatgg
    63301 cgacgtggga ggggattttt ttccccaacg cctcacaacg atccgtcggc gcttccggca
    63361 tcttttttga cctcatccca gcgtcttttg acctcacgga tatccttcca ggtcaaatct
    63421 ttcgggctcc ccacagctcg ggactggttc cgaagaagat agctcgggtg gtacatcggg
    63481 aaaacctcga tccctttcca cttaaaccat ttccccctca attccccgat cggtgccgtg
    63541 gtcttcagga tccaccgtgt cggagtgctg cccagcagga cgaggatcct tggcgaaacc
    63601 agggcgatct gcgtttgtag aaaggtgtcg caggccatca tttcttccag ggtgggaact
    63661 cggttgccgg gtggtctgca tttcacgacg ttgccgatga agacctcacg tcggtcgatt
    63721 ccggcagcag aaaggatggt cgtgagaagc tgcccggctt ttcccacgaa gggcaagccc
    63781 tgttcatctt catccgctcc cggcccttca ccgatcagca tcagcgaagt ttctctcgga
    63841 ccgtccccaa aaaccgtccg gttccgcgtt tcagccaggg ggcaaagccg gcagcgagaa
    63901 acctcctcct gcagccggga ccagaggatc tccctgcggg agaagatttc ttctgctgtt
    63961 ggactaaaaa tcgttctatt ttccgcctgc atgcaaatca acctcggcca cgacgacatc
    64021 aaccccgcgg acatccagtc ccatgaaata gctgacgctg gaggaaattc gagctttcac
    64081 ggtcctcgcg atctccacca ggttcctgtc ccccaggatc acgccgattt tcatctggat
    64141 ggaaaccccc tcagaccccg gcttgatccg gacttcctgg acgcttttga cctggggagt
    64201 ccgcaacgcg acatgacgga ccaaatcctc gatcgcctgc ggttcgatcc gcaaggcccc
    64261 gataaagctg aaggggggtc tgacgatcgt tttttcccct tcgtaccggt tcttcccttt
    64321 ccagaaaacc ctcagtttcc ccacgagctt gccggcgaag ttctttcgga cctggacctg
    64381 ggaaaccgga atgacatgct gccccttgac cttcctctcc agtcgcgccc tgcggatttc
    64441 ttcgggggtt gccacatcct cgatccggat gacgttttcg ggcggaggga ggccaagtcg
    64501 ccgggcaatc tgtgcagcca ttccttccga agtggcgacg atcataacgg tgcagggctg
    64561 ggcctgcttg aaaaattccc tgacttcctg tcggtgatcc gggaattcca tcatggccct
    64621 ccggattgca cgaacctggt tcctttccgt ttttgcgctg cgcccgcaaa cgatttgccc
    64681 cttgcggatc acgagcccgt cgtccaggat gaaatcgatc tcgagatttc tcgctaggac
    64741 ctgagctcgc tggcttttcc cggttccagc ccgtcctacg aaggagatga ccctgacgcc
    64801 ggaaataggg gcggtttcat cgtcctgcaa tccgcttcaa cacctttccg agcccctgaa
    64861 ggaacaggtc gttttcttct ggcaggccga ccgtgaccct caggtgtcct ggaaagccca
    64921 ggtcgtcgcc aaggcgaaca atgatccctt ccatgagcag gttttcgaaa accgtcctgg
    64981 cattcgggaa gtcgaggata atgaagttcg tctgcgtggg ttgaacatgg atccccatgt
    65041 cgcgcagggc gttgctcatt cgctcgcgct ccgccagggt cctctcgcga acctcctgga
    65101 tgtaggccgt ttcggaaagc gcggccagcg cggcgttctg ggcgatgctg ttgacttcga
    65161 acactttgcg gaccttgaga taggtttcca cgacgggttt cggagcgatg gcgtacccga
    65221 cgcgaagggc cgcgagtccg taaattttgg agaaggtccg gatcatgacc acgttcccga
    65281 gctgatcgaa aagatcgatc ccggaagcga atttcggatc ttcgacgtat tccgcgtagg
    65341 cctcgtccag gatgaggagg gtttctcttc cgttcagcga gttggcaaga gccgtcaggt
    65401 ctgcccggtt catgatggtt ccagtcgggt tgttggggtt gcagatcacg agtgctttcg
    65461 tcctcggccc gcaggcatcg gccagccggt cgatgcagga gcctcccccg tcccactcgg
    65521 tccggatctc gatgcgtttg gctcctgcag ccgctgcggc gaggccgtac atcgggtaag
    65581 tgggaacggc ataggcaacc tcgtcccctt cgtcgatgat cgactgaaaa agggaataga
    65641 gaatgccttc ggtgcccgct ccaacgatga cttcggaagg ggaacggccc catttcatgg
    65701 caatggccct tttcaggcgg taggaagcgg ggtcggggta gcggcaaatt tcttcagacg
    65761 acgcgcggac cgcttcgagg accttttccg gtagaggcca ggggttttcg ttcgagcaga
    65821 gacggatcgc ggattgcagt cccagctccc gctgtacttc cgaaatcggt tttcccggcc
    65881 ggtacggctc aacgtccgca agggatttcc tgacgatatg gcttttccag gacacgatga
    65941 tcccctcccc gctctttttc tctagaattt tggcccctcg ttttcgtcgc aggggaccgt
    66001 ttcgacttct cctccgaggc tttgcagctt ctcgaccatc atctcataac cccgcagaat
    66061 atgccccatt tggtaaatgc aagtatagcc gctcgcggcg aggcccgcca ggacaagggc
    66121 ggcccctgcc cgcaggtccg tggccctgac ttccgccccg gtgaggtgtt cgactcccgt
    66181 gatgatcgca gtatccccct gaagttcgat ttggcagccc attctcttga gctcactcac
    66241 atgaagaaac cgggactcga aaacccgctc ctggatcacg ctcgttcctt ttgccaggca
    66301 gagggcagcc atgatctgcg gctggaggtc ggtgggaaaa cccgggtacg ggagggtttt
    66361 gagggaaacg gcggacaggg ctccacccgg aagggtccga acggaatcct tcccgatgga
    66421 caggcgagtt ccggcttctt cgagtttggc taaaagagcc tccaggtgtt cgggttccat
    66481 ccccgtgacc gtgacatcgc ctccggtgac
//
LOCUS       DAOOVN010000002        62691 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_260_length_62691_cov_13.593030, whole genome shotgun sequence.
ACCESSION   DAOOVN010000002 DAOOVN010000000
VERSION     DAOOVN010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 62691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 62691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62691
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_260_length_62691_cov_13.593030"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..1065
                     /locus_tag="PLY83_00350"
     CDS             73..1065
                     /locus_tag="PLY83_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HOV28518.1"
                     /translation="MTINPSITDVLRAYRFLRNRVRHTPVERSFPLSERTGADVFVKW
                     ENTQFCGSFKIRGALNKMFSLTPEERARGVVTPSSGNHGQGVAFAAAQLGVKAVVCVP
                     GACPRTKRQAIQRLGGEWVELRVVGQLYDDAEHEAHRLAEAEGMTFVSSYEDAFVISG
                     AATVGLELMMDEPELDLLLVPAGGGGLMNGVALAARTLRPDIRIWGVQSVASQPWVAS
                     WEGGRVVEVEYGDSLADGLTGSIPQSLLDLAKTRMKGCFAVTEDEIAQAIAFLHAEHH
                     QVVEGAGAVGVAALLAGKVPEVLSGLRVGVVISGGNIDHERLMTVLERWGKGRA"
     gene            complement(1062..4682)
                     /locus_tag="PLY83_00355"
     CDS             complement(1062..4682)
                     /locus_tag="PLY83_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV28519.1"
                     /translation="MRGKTRSPAKLAFWSFLILIPALLAVAAVRMPYGEGIVRNLAVQ
                     GAKSRLGADLYIGSMSGNPVIGYKARDIVLTREGLPVLSAAELTFRQDLPSILQRRIF
                     IRRLDIGGADVDIDRLREILPEFRQETDRINLPLDAVRLDDCLLRTPFGRIDIRKATL
                     KISDFAVDATFRAEVNGVETRGRGRYRLVGGLAQIENLEMEAGKGHIEVFGTVRPNLG
                     LLVRLSGVELLTLRNFWPTLGDRFFGPLLTEFRVEGRWPDLSYSGDLSLRSGRVAGID
                     FENLKSRWAFDGKILSFEGVSGNAFGHPLTGTVSADLSQNPVPWTLRVAGEDMEISRW
                     KTVLPWIGFLKGTARKVSIDLKGPLRSPSGKVEAEAEAMSIASQDLTAARAAFTVEEG
                     RTGNLELSGKWLDSPVEARGPVQIRPRVELDLRLKLQGVDFDRLATRFKGLSELKGKG
                     ILGADLRLFGPASSPRYEGQIRSERLEALGESLQGLRVAFASDGKNARIQEGTASWQG
                     GKASVSGLVEGLFAPKPRLTLSGRMMEVDAVRVANRFGALRSLGLAGKIGGSFRVAGS
                     TGELSLEANLSGVQARLLPLFPLRRFDLSFRFRPDLLEILSMSEAGRSPSLFLSGRIS
                     RLSSRPEADLKGGFSRMEIAPFLEGLLPIPVSGRVEGRFSLKGALSSPAWSAEGSSES
                     LTAGGLPVGGFHFSLGGADDRFRLEKLEGEVIGGRVKGSGQVRRAAASRPAELDLEAS
                     AEGLDLKEITNRYSLALPIRGLASAEATVKGPANRPTVSLLVRVPELNVSGLFFRQVQ
                     VKGEGASTGEILHIREARALVGDSPVNGSGTIVKGREGFELAFSMEGSRLELQDLVPR
                     FISGARGVLRGTVDTRVSGRISAEGFQGSGRLKSPELVLWGFHLTDVDAPFATTDDFI
                     TVESGTAKAYGGDVKAAGSLEMGKARWGARMTVRGAELAPALKDALKTEGTVSGKADL
                     DFRISNEYGKAFLMDGHGLLKVKDGAVEGFSALKSLVPITGSPSIRYSSVNANFNIDG
                     KSVFLLPGTRANAVPGDRFYRYLSVDGSIGEGGSLDLKSYGEINVRGLNLLLGGLEGL
                     LSSDGSWSTATLQQFLGGALGGAARRDFQEVSFNIKGPWRKPAISDLRVIQHRKESPI
                     PSSPSDPAEKADQNQMRITITIPTGEGGTRTSEDPTTQIGDQLLEQIIKQILKPGNGG
                     EN"
     assembly_gap    4710..4809
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4901..5740
                     /gene="larE"
                     /locus_tag="PLY83_00360"
     CDS             4901..5740
                     /gene="larE"
                     /locus_tag="PLY83_00360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HOV28520.1"
                     /translation="MGYGGSESPGREQKWLRLLGLLKEKKNAVVLFSGGVDSTLLAAA
                     AFLALGDRAVALTFRSPLEDPSEAERASSAAERIGIRHVCLESGDLSVPEIANNAPER
                     CYACRKNRDARAWAWARESGFTSVVDGLSLSDLAEDRPGRKASDEDGIGHPLLEACWG
                     REDVREFSRQLGIEGADRPGSPCLATRFPHGTFLSEERLRQVGRAERALAALGFSPVR
                     VRSFPGFGAVVETGDPAGIAARKEEVLRILKEAGFPAGFVDLEGYARGKMSRLPADGN
                     YPR"
     gene            complement(5728..7353)
                     /gene="pruA"
                     /locus_tag="PLY83_00365"
     CDS             complement(5728..7353)
                     /gene="pruA"
                     /locus_tag="PLY83_00365"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HOV28521.1"
                     /translation="MVLVWKAEKPKNDVDSIPLYAPGTAERELLLGELERLQKAPEEI
                     PLFIGGRRSWEGEIQEIRSPADNRLILGRARLAGEKEIDSAVESALQAWKSWSSMPWR
                     DRAAVFQKAADLLAGPRRTRNIASIMLNLSKTPYEAEIDLAELVDFWRFNAWYLHLLY
                     GMQPEQARGEINRFDWRPLEGFVFAVSPFNFYSIAGNLPTAPALAGNVAVWKPARAAL
                     ACNWEIMKVLEEAGLPAGVINFVPFSSRYAPRILEHPDFAGLHFTGSYDTFMTLWRTI
                     GKNVDLYRSFPRVVGETGGKDFAVVHASADPRIVAVSLLRGGFEYQGQKCSALSRAYI
                     PESLWPEIRKILLEEIPRLRVGPTSDLGCFMGALIDEDAYRKTVRYIEYALSRPEEYE
                     RVFGGSYRNEEGWFVEPTLFVTNNPHGKLMREEIFGPVVTVFVYPDERYGEILELCDS
                     STPYALTGSIFARDREAIQKAACSLRFAAGNFYINDKPTGAIVGRQPFGGSRHSGTND
                     KAGFLNNITRWLSPRTIKETTVPPTDWRRPYLG"
     gene            complement(7512..8339)
                     /locus_tag="PLY83_00370"
     CDS             complement(7512..8339)
                     /locus_tag="PLY83_00370"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOV28522.1"
                     /translation="MPGPKRPFILLFLLLLLTLPGNSAGPLLPLGTAWGETILGTVTT
                     VFDGDTVEVLLDTGRIEKVRYLMIDAPELHHPKREVEEFGLEAALANRDLVLGKRVRL
                     ETDVQPRDRFGRLLAFVWIDRPNGSILVNEKLVEEGFALPFTLPPNVRFAGRIRSALY
                     RARNGGKGFWGTAAGRLFTPKQVWADLPLLAGSFITLEMRVDGMTRFKTGWRLFEQGT
                     RTALVLYDEPPGLFGSIGELKGARVRVLGKVQASYRGAEIPISDPLQILSVFHPARP"
     gene            8442..9137
                     /gene="rsmG"
                     /locus_tag="PLY83_00375"
     CDS             8442..9137
                     /gene="rsmG"
                     /locus_tag="PLY83_00375"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOV28523.1"
                     /translation="MRTAELLPLEAALEEARANRDRLWKYCEALAVGNTRARLTGPAD
                     PETLWREHVLDCAAALPLLPQEGRVVDVGTGGGLPGVVWAICRPGLSISLLDSVSRKC
                     IALQGIVDSLGLRNVTVVCLRSEELAGKDREGFDIAAARAVTAAGPLAELLSPLAAVG
                     GTLLAFKGPRVHEELDAVGEKWSRLGLGDPVLYPYPLDGKERVIVRWPKVKPTPAAFP
                     RNPAMLERKPWWR"
     gene            9142..9912
                     /locus_tag="PLY83_00380"
     CDS             9142..9912
                     /locus_tag="PLY83_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOV28524.1"
                     /translation="MLVMAVANQKGGVGKTTCCVNLSAELGRMGVRTLVVDADPQGNS
                     SSALGIDRNSLERSVYDVIAGNLSASEAVVATAWENVFLLPATLDLAGAEVELAGAMS
                     RESRLRRKMADLKGFDLVLIDCPPSLGLLTVNALVASDGILVPVQCEYFALEGLGQLL
                     RTMDLVRSYLREDLSLSGLVMTMFDGRTRLSREVVEEVRRQFGDLVLETVIPRNVKLS
                     EAPSHGRPIGYYDPLSAGAEAFRELAKEVSQRWLGRKL"
     gene            9891..10769
                     /locus_tag="PLY83_00385"
     CDS             9891..10769
                     /locus_tag="PLY83_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOV28525.1"
                     /translation="MAGQKALGRGLGALFPGGENATAKAVPVSLPIGEIRPNPAQPRK
                     RIDPDQLESLAESIRQHGIIQPLVVRPAGEGYEIVAGERRWRAAGLAGLKEVPVRVLV
                     MNESQSVQAALIENLQREDLNPLEVADGIRDLISRFAISHEEASRRLGWSRAAVTNKL
                     RLLSLPDPVKAMLADRSLSEGHARALAGLDDPVRVTRLAERIVRQGLSVRQTEILVQE
                     EKERRLDPPPREKNEIPLPEDFAVFSKEKGFKASLKRKGKGVSLVLEGLSQWQAEELL
                     DLIRTGGDRVFQAEKG"
     gene            10773..11003
                     /locus_tag="PLY83_00390"
     CDS             10773..11003
                     /locus_tag="PLY83_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent proline transporter"
                     /protein_id="HOV28526.1"
                     /translation="MPFKMEDLAAFSKILSAALVICGFIVLGLLLGRQLAGKGAPAWV
                     VPATTALGALVGMSQGWAMIRPLWKDRGKKRP"
     assembly_gap    11041..11140
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11197..11685
                     /locus_tag="PLY83_00395"
     CDS             11197..11685
                     /locus_tag="PLY83_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOV28527.1"
                     /translation="MDTLYSPWRMAYIESAEKTPGCIFCEFPKKGDDEAFLILHRGKS
                     CFVIMNAFPYNPGHLMVVPYRHIAEYENLLDEELLEMNRLAGDCIAALKKAMSPHGFN
                     MGVNLGRIAGAGVEGHVHLHIVPRWSGDTNFITVTGQTRVLPEALPVTWAKLREALRA
                     RE"
     gene            11678..12301
                     /locus_tag="PLY83_00400"
     CDS             11678..12301
                     /locus_tag="PLY83_00400"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOV28528.1"
                     /translation="MNEGEDRFLEPAAEIEIEMTEKRSRFIAAVRMARTPPEAKEAVR
                     QIASARPDANHHCWAYRVGLPPVEYYSDAGEPAGTAGKPILGAIQRAGMTNTVVVVTR
                     YFGGIKLGVRGLIEAYGAAARKALEASGTVWKVASRTSVIRLSYEEQGNLRFYLRELG
                     VEAESLNCRYEEAVIMEVPVPLSMEERASALFGSLLARGQILGWEWR"
     gene            12337..13842
                     /locus_tag="PLY83_00405"
     CDS             12337..13842
                     /locus_tag="PLY83_00405"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HOV28529.1"
                     /translation="MIQEETQDSLTRRLRHPVCRSLLRDLTEEELRHLSATDCVPNNR
                     NAALYVTRIRSRSAAFTENVYDIDEGTISLLEQVWGYLRWQQLIRVSARVGSPEGSPL
                     KANLYVTRRFSRIAHMFYGNFFPQVDEDFADMVTVVVPEWHQRKVLVFPNQRVTYILG
                     SDYYGETKMATLRMAMHIAREELNGIGLHAGSKNIRLQSESGMKELGLLVFGLSGTGK
                     TTITTADHGLKAPEGVEVLQDDINLLLSDGRSLGTERNFYIKTDNVSKQPPLLAAAMD
                     SRAILENCWVDDDGDINFDNHAISTNGRAVVPREAIPNTSQRIDLPHTDILLFNMRRY
                     DIPPIGRLVSPEQAAAYFMLGESTITSAEDPSRVGQAKRVVGFDPFIVNQPQKNGNRI
                     LQILRENPQIRCYVMSTGRVGGNSGANITPEVTFACIEAALRGQLQFRYDDILGYEIP
                     ESYPFEGGERFDPYRVWDSETYRKMIGDLRKERREYLGKFSGLAQEIVTAV"
     gene            13953..14513
                     /locus_tag="PLY83_00410"
     CDS             13953..14513
                     /locus_tag="PLY83_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HOV28530.1"
                     /translation="MPNRTRTMILASLFAVLTAVGAQVRIPLPLVPMTLQVLFVFLSG
                     VLLPPRAALTSMILYLAMGLLGLPVFAGESGPSVVLHPTFGFLLGFILAAWLISLIVT
                     LKGQRFFVCLTACLAGLAAIYALGLAGLYLNVNLVMGKSMTWAQAAKVGMVPFLAGDL
                     LKGVAAAFIASRIGPRLSAAGKGNGH"
     gene            14598..15695
                     /locus_tag="PLY83_00415"
     CDS             14598..15695
                     /locus_tag="PLY83_00415"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOV28531.1"
                     /translation="MNWQERIRTDCPACGQRYETDTRPIRCGCGSPLNTTVEWEEMRI
                     DGRAHGLWRYGSALPFELAEAPSMGEGWTPLVRARESENLYYKLEFLFPTGSFKDRGA
                     VMVMAEARFRKASEVIQDSSGNAGASIAAYASRSGIKATILVPAGTAEGKKDQVLSYG
                     AELRCVDGDRTATAREAWKLAQNRFYASHVWNPLFLEGTKTFAFETWEQLGFQAPDVL
                     FIPAGNGTLLLGAWKGFSELLARGLIERLPRLFACQSFRCAPLFSRLHGKPFAGPGET
                     VAEGIAVSVPPRIAEMERAVAETGGACVLVKDDEVLQEQKRLAKEGFLVEPTAAVASA
                     AALKAMRERRLKEEERIVVPLTGSGLKKTPA"
     gene            16084..16833
                     /locus_tag="PLY83_00420"
     CDS             16084..16833
                     /locus_tag="PLY83_00420"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOV28532.1"
                     /translation="MKDVETLAEWILEARPFVVLSGAGMSTESNLPDFRSRQGLWAQY
                     DPARLATEWAMENRPDEFYAFYRMRVEALKASSPNRGHRILADWERRGLLSAIVTQNV
                     DGLHQEAGSRSVVELHGSLRTARCRGCGAKSPGVRLLEGPFCPACGGRLRPDVVLFGE
                     NLPEAPLEEAERLASTARLFVVLGSSLAVSPANRLPGIAAACGAKVAIVNREATPFDG
                     RAHLVIQRGIGEVLAETDRIVSEKESLKGPV"
     gene            complement(16816..17952)
                     /locus_tag="PLY83_00425"
     CDS             complement(16816..17952)
                     /locus_tag="PLY83_00425"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOV28533.1"
                     /translation="MAGKIAVTLQEASLSEQAHFLQLLKDAGFSIEAAPSAPGDEAPL
                     PLLALGKVLTQSDPSGEDLAALVEGVTRVIQLPVSLFLPVQEKTLLSCRAAAPGTPPA
                     WIEAEFPAGSAETVLKVQGEIRPCLFPLDKTELSAGFLVAALRGSSGEEKAFSFLNEL
                     AGAIGEARARTERLRALDRGASLLDSSVDALVEACTTLLELRGHETAGHSERVTSLSV
                     KLGERLGLSSRELIDLRRGALLHDIGKLTLPDSLLQKSPPLSEEDWKILRTHTVRAFE
                     AFRSVPALQGALDVPLRHHERFDGSGYPDGLKGKDIPIVARIFAVVDVWDALLSETCY
                     RKRWSPGKVLDHLRRAAGVHFDPEVVSAFISLLEEDPSLLDRPF"
     gene            complement(18011..18391)
                     /locus_tag="PLY83_00430"
     CDS             complement(18011..18391)
                     /locus_tag="PLY83_00430"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOV28534.1"
                     /translation="MTKLFSGVPLAALGPDGRTWFEPVWAAATFRERFRGLMFTDPKP
                     MGVWFQGCRSIHSHWMRYPLDILFLGEDGAVLRHVVLNPWSFASHPHAASILEIPLGY
                     ANTKALPPRLSLVPADTGLSPGSL"
     gene            complement(18388..19278)
                     /locus_tag="PLY83_00435"
     CDS             complement(18388..19278)
                     /locus_tag="PLY83_00435"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOV28535.1"
                     /translation="METWISMVLFAMLGLSLWLFFQAFLPGEDVRLQRASEYLEVQRR
                     TETPSFRQRILSPLGKALVDVLSRLYPPRTLERLDEYCERAGRPLGITGKALAVSKVV
                     SAFLLWTAAGLFVRGRFFLPASILAACTGYLLPGLWVTSRTRRRVELARAQLADAMDL
                     LVVSVEAGLGLDAALQRVASRFRHPLGDLFSKAMAEISLGSPRESAFRGIAEALPFDE
                     IKSFTASIIQAERLGTSIAAVLRAQAAGLRKRRRLRAEEHARKAPVKILFPLVLFIFP
                     SLFVIILGPAMIQILENLRP"
     gene            complement(19293..20225)
                     /locus_tag="PLY83_00440"
     CDS             complement(19293..20225)
                     /locus_tag="PLY83_00440"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOV28536.1"
                     /translation="MQDLLVALLSVALGLASLLCVFRAFRPVAGTDPIERVKRWFTQD
                     RPSNEERQRLRFVSSLAPRQLQRKVSRLLQNAGIPLEVEEALLLWAILLLACTLSGFL
                     SAGVFGLVAGLASGGSLPLLFLRVQSARRKRQMEGQIPDLLDLLASGLRAGFSFFQSL
                     QHAAGQMEAPIGPTLRQVITEMTVGFDVETALRRWVERTGSLDLELAVSAILIQREVG
                     GNLAEVLDNISGIMRDRREAQMEMRSLTAQGRMEGWIISLLPVVLGIFFTLTRPEYMG
                     VLFHTPQGVRMLTLSGVFGVIGIFLVNRIVKPRY"
     gene            complement(20238..21575)
                     /locus_tag="PLY83_00445"
     CDS             complement(20238..21575)
                     /locus_tag="PLY83_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HOV28537.1"
                     /translation="MSLLKRLAGRSAEEQKERIQVSPAFRAAQRRLLVRLAEEVDPKD
                     LGGSDPGSQGNAELRRKVVRIVDSFLSASPELSAAEQERLREEVLNEVFGYGPIQPLI
                     DDDSISEIMVNGPDIVYVERRGNIELTDVVFADDEHVKRVLERIVSPLGRRIDESSPM
                     VDARLPDGSRVNGVIPPLSLEGPLITIRKFRKTPLTTADLIAGGTLSEEAVAFIRTAV
                     EARFNIVIVGGTGSGKTTLLNVLSGFIPEKERILTIEDAAELKLQQPHVVRLEARPAN
                     IEGKGAIPIRSLVKNALRMRPDRIIVGECRGGEAFDMLQAMNTGHDGSMTTIHANSAR
                     DALARLESMVMMSGLELPLKAIRDQIASAVDLLIYQERLRDGSRRVMTVTEITGMEGE
                     IIQTQDLFHFDLQGFDERGRVRGKLRPTGIQPKHLAKFELAGVALPADCLAGR"
     gene            complement(21572..22702)
                     /locus_tag="PLY83_00450"
     CDS             complement(21572..22702)
                     /locus_tag="PLY83_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV28538.1"
                     /translation="MIRLFLAGTEERRNGWKRGLQESSTIEVAGEAGSFAELRSLVQA
                     ARPHVLLMGLCAGEQEAADLLDALKSLQFLPGIIVAAEENDPEAIRTIIRLQARDFVP
                     AACPVAELDAAVNRAYSAAVGQNRDRTGVLLGLWGCRGGAGTTSLALALAGAARRKGL
                     KVAVADGDIALGDTAFFMNARPTLTWVDWARDSSGGERDIHRYLLTTEEGFSILAAPK
                     SPAQAELIRPQAVSEALDLLQKSHDLVIADLHRSFDEITLHIAERAQRLWLVTDASLT
                     GVKNVSLFWNLMDQLRLVHEERCAVINRVNKGNAQTAAKMASLYKASALIPEEPALDR
                     AWESGRSPVLTLPRSPFVKAVGELLNRLFPDSSPGGKGRKSR"
     gene            complement(22699..23880)
                     /locus_tag="PLY83_00455"
     CDS             complement(22699..23880)
                     /locus_tag="PLY83_00455"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HOV28539.1"
                     /translation="MRKKRTFPVLVLATVLLLLSQPLPSAGGPAQPQQTLHLETGENA
                     PLTFQSLARVAIGNPNVLDAQPVNRNDLLLFGKSEGVSSLVVWDSKGCHVFDILVHPS
                     RRVRTWDLEQMLQDSGIKVALTENTVILEGAVRTPYEKKRAEAIARGFAPNVVNLLNV
                     EGDPRVKIEAIVMEMAKGNGQKLGLSYLEGGRGQLVSGSANLVNSELGQIGFTWNRAA
                     DITSNLKGLFDAVKNTSSTRILSRPSVSGLSGEKAFINVGGEIPVPVGVENNQIKVEW
                     KPYGVIMTIVPEVLSDGDILLNLSAEVSELDWENRIVTNGVSIPALRSRKVENRTVLK
                     DGEPLVIGGLLDNKQTRMVKKIPLLADLPLLGSLFRSKQFQNNETELVITVVPQVVKN
                     P"
     gene            complement(23894..24775)
                     /gene="cpaB"
                     /locus_tag="PLY83_00460"
     CDS             complement(23894..24775)
                     /gene="cpaB"
                     /locus_tag="PLY83_00460"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HOV28540.1"
                     /translation="MRRKILLLTLSLLLALIAAALVFGQIREYRKRLVAAENRMIPVP
                     AAAREIEAFSTLTPEDIVVLKMPRGAFPAEEIPAADQLAGKRTLYRLAKGDPILKFKL
                     AEAGGKGSLSLTLPNGMRAIAMAVDEVSLVSGHIRTDDRVDVVILEKRPGGITPARNL
                     LSGLKVLAVGVDAGIPAEEREKAAPAELAKSVVLEVSPQQAPILAWAQRRGDLWLSLR
                     PAGDTGKTPEAVYAGPDPAAWKEQPRPGGDTAQAQSPSKPVSSPHPETSAGRPTRGWA
                     VTVMVPGKTESVAVPVQ"
     gene            complement(24788..25753)
                     /locus_tag="PLY83_00465"
     CDS             complement(24788..25753)
                     /locus_tag="PLY83_00465"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tad domain-containing protein"
                     /protein_id="HOV28541.1"
                     /translation="MGQFRQKQGGGILVLTALSLGILLSAASLAVDYGYAVLMRERLQ
                     RAVDAAALSGAMELAMGHPQTAADRAVQMGNENLEASYSVRFPASNRCHVDGEKALRT
                     FFAVFLGKDTILVRAHAEAIASPVSEVQGLRPFAIVPPEGGFTYGVTYVIKYGPGPGS
                     GAYHGNFNAVALDPTDRGAKDYLQNIKYGSDGWIRIGSEISTDDDWIYTEPGNMAGPT
                     REGVNYLVGQDSHDWDYYQSMPGIETSSRLITIPVIESMADINGRELVHVIGFARVFL
                     ENAVGTGNDVDIYARFVKMATPNSRTARNVPDYGAYGIRLVVPEP"
     gene            complement(25756..26148)
                     /locus_tag="PLY83_00470"
     CDS             complement(25756..26148)
                     /locus_tag="PLY83_00470"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HOV28542.1"
                     /translation="MNGEKHRGAALVELALTLPVLLVLVMGIIEMASLFQQFQVVQHA
                     AREGARLAAVGESPEEVTAAVLNLLSLRSFAGTPTIRVEEVTLSSYVESKVSISVPLR
                     TVTPLGQFVPALLGTFRPTAVAAFRKEG"
     gene            complement(26145..26651)
                     /locus_tag="PLY83_00475"
     CDS             complement(26145..26651)
                     /locus_tag="PLY83_00475"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HOV28543.1"
                     /translation="MAAFWIFLPASVFGGLWDLRFRKVPNLLNGAILLGALAWHAYRG
                     DLVSSALGAAAGFSALLLPYWLGGMGAGDVKFMAALGAAVGWPSAADTLLWSALSGGV
                     LVVVLALRPEDWTQAWLEAASGWKEAVRTLCRNAGSRKREKVPYALAMTAGFILSHFF
                     PLLGRVTA"
     gene            complement(26749..26931)
                     /locus_tag="PLY83_00480"
     CDS             complement(26749..26931)
                     /locus_tag="PLY83_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HOV28544.1"
                     /translation="MMKKLIGLVRDEEGQGMVEYGLIIAVIAVALVAALVTLRGGISG
                     VFQNASDQMSSNSSGS"
     assembly_gap    27010..27109
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(27155..28969)
                     /locus_tag="PLY83_00485"
     CDS             complement(27155..28969)
                     /locus_tag="PLY83_00485"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOV28545.1"
                     /translation="MAVLVASRSVPGAEPLLRSLRAEGIEGHAWEVGTELPGPESARW
                     DAVILDLRAPWPVKARFLPAGPRVAVLDPAHEALAAVARERGARQFLYDASPSVLAEA
                     LRRLSAETIEARGSLPEILLQTSLPVALFHPVYGLESCNLAFCERVRARREELEGLDL
                     VSLFGQEQARSLSGLFTGLLLGERETEWRDLCFSLPARGEVPAEASFSPVFRDALDRA
                     RILATLAPEGRTRQWEGCLEARSRKLAVLQSLNLALNETWNPRSTLKVLVNLILSHAD
                     VDAAAVSLLDEATMKLKFAEGEGFWGSAIQSRFSDVGEGVGGEAALRRQTVLLPDLDN
                     PGVTRVEPFLLNEEGFRAACAAPLLAPDRLLGVLEIYSRKSLEAPEALKEMTETVARQ
                     ASAALHRGFLVEELERANLELNLAYDSTIEGWSRALDLKCREPEWHTKKIAELTVCLA
                     RRLGMEGKDLQAIRRGAILHDIGKMGIPDAILTKPGPLTEEEWKVMRLHPVYALQMLQ
                     PIGFLKDSVAIPYSHHERWDGQGYPLGLRGEGIPFFTRVFSVVDVWVSLTADQPYRKA
                     WTPERALAYIREGSGRLFDPLVVRAFLEMIESEPGKNP"
     gene            complement(28974..29762)
                     /locus_tag="PLY83_00490"
     CDS             complement(28974..29762)
                     /locus_tag="PLY83_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOV28546.1"
                     /translation="MNSVCIPVSDLALQRGVGVFDSLRTLGKRPLALSLHLDRLEHSL
                     REAGIAPPASREEIERVVREGISFMEEETSIRIYVTGGDRCEGGRFPSPRWFVLFEPM
                     HRIDPSATAEGVTLWPLPHERPLPLLKSINYMAAFVERGQHPEAFEILYCPGGEITEA
                     TSSNAFMVIDGKLVTAPLERVLSGVTRAFVLEVAREAGYRIEERCPRLEELPRAREFF
                     ITGSVKEIVPVIRIGTTRVGDGRPGPVTRHLTQRLASSIERWVE"
     gene            29958..30653
                     /locus_tag="PLY83_00495"
     CDS             29958..30653
                     /locus_tag="PLY83_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOV28547.1"
                     /translation="MEFIAQGFSQAVTLLLSGDRETWSAIEVSMRLTSLSMLASLLLG
                     LPLGILLGTRNFPGKGWVRVVVDTCLALPTVVVGLLVYALISRQGPLGSLNLLFTVRG
                     MAIGQTFLALPIVVALTASALEGADPRMLLTLQTLGTPPGKMILALLREARYSVLAAA
                     VTAYGRVVAEVGVSMMLGGNIKWHTRTITTAITLETGKGEFSMGIALGMVLLFISFAL
                     NGALSLFRRRSAS"
     gene            30650..31675
                     /locus_tag="PLY83_00500"
     CDS             30650..31675
                     /locus_tag="PLY83_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOV28548.1"
                     /translation="MSAPVYRLENLQVRYQDRLVLDVEHLEIGEGSILGLEGPNGSGK
                     STLLRVLAFLEAPSSGSLHFMGSPADSCDLLALRRQVTLLLQDPYLLRTSVFENIAYG
                     LKARGIREGLKDRVFHSLELVGLSPGVFAGRSWKSLSGGEARRVALASRLALRTPVLL
                     LDEPTAGVDADSAILLKSAALKAREEWGATLVVVSHDMAWMSQVADRLLAFQDGRPGR
                     YVVQNRFEGPWFEQEGGLVSMKIGDGQSIVATKPPASGRTGFLDAEDITVAVLKPQGL
                     STRNAVRVRILQLSLENGSGAVLLKGACGDRILTARLTRSSAAELGLVPGTEAWFLFK
                     ASAFQWE"
     gene            31874..32962
                     /gene="buk"
                     /locus_tag="PLY83_00505"
     CDS             31874..32962
                     /gene="buk"
                     /locus_tag="PLY83_00505"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOV28549.1"
                     /translation="MGFQVLAINPGSTSTKVGWFDDGVEVWHSTVTHDPDELAAFPTM
                     ADQFGFRMAAIEKACEEHGNHFSQLAAVVGRGGLLDPIPGGTYAVDALMKVHLRRGKP
                     WEHASNLGGILADAIAAPLGIPALIVDPVAVDELDDVARVTGLPELPKRSLGHALNIK
                     ATVRRAARDLNGDWKKMDFVVAHLGGGITVCAHRQGRMVDTNNANEFGPFSPERAGGL
                     PAGDLARLCFSGRYTLQDLRKRLVGKGGLVAYTGESDVRKVKALIAGGDESAALAYRS
                     MAFQVAKEIGACAAAMGGKVDAILLTGGVAHDSDFVEMVVGRVQWIAPVLLYPGEDEL
                     LALAEGAMRVLRKEETPMRYGDFVKTEA"
     gene            32969..33874
                     /locus_tag="PLY83_00510"
     CDS             32969..33874
                     /locus_tag="PLY83_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HOV28550.1"
                     /translation="MQLKNLDFLLEKCKAAPRMTLAVACPQGDDILEAVADAAQEGVV
                     DPVLVGNRGKTLAIAESLGLDISGLEFVDEPDDALAAEKAVRMVSSGEAHLLMKGNVK
                     TAVLLKAVLNKEWGLRSGSLLSHLFLFQVPRMPGVIGMSDGGMTMYPDLPTKVGIVEN
                     AVWAYHRIGVECPKIAALAAVEVVNPDMQATLDAAALAQMNRRGQIKGCLIDGPLALD
                     NAVSAEAARIKGIESPVAGDADMLLVPSIEAGNFVGKVMMYMSGGKGAGVILGARRPI
                     VLTSRFDNAETKRLSIAFGAVLAMS"
     gene            33913..34842
                     /locus_tag="PLY83_00515"
     CDS             33913..34842
                     /locus_tag="PLY83_00515"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HOV28551.1"
                     /translation="MEQLRSLTALLDYAKRIGAEKGPRKVAIALAEDAGVLEAIEAAR
                     REGFAFGILVGNSDKIKAEADKAGIDLANYEVVDERRGEAQVGLTAVQLVSSGKADIF
                     MKGMIHTNYFLKALLNKEIGLRTGKTISHCYFHEVKGYDRIFFIADAAFNMYPDLKTK
                     ADILVNTVGLARSFGVECPKVAVLAAVEVVNPDMPATLDAAAITQMQRRGQIKNCLVD
                     GPFALDNAVSEESAKHKGIVSEVAGKADILLVPNIDAGNMMVKALVYFSENKTAGLIL
                     GASAPVVLTSRSDTIEAKTLSIAAAVALSVFKG"
     gene            34887..35984
                     /gene="buk"
                     /locus_tag="PLY83_00520"
     CDS             34887..35984
                     /gene="buk"
                     /locus_tag="PLY83_00520"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOV28552.1"
                     /translation="MGGVALEKGKYRILTLNPGSTSTKVALFEDETELWSDTQRYDAD
                     LVRTFANIMDQESFRLDAVRQALARHETPVSSLDAVVGRGGLLKPIPGGTYTVSPAMI
                     EDLKSCRWGAHASNLGAPMAARLAAEAGHERAFIVDPVVVDELVPEARLSGIPEIERR
                     SIFHALNQKAVARRAARDLGKAYAEVNLVIAHMGGGISVGAHERGRVIDVNNALDGEG
                     PFSPERAGSLPAGELVRLCFSGTYGLDQIMKKLVGGGGLVAHLGTNDLREVERRIDEG
                     DEKAGRVYEAMAYQVAREIGARAAVLKGQVDAVVLTGGLAYSSRFTDRIASRVAFIAP
                     VRIYPGEDESAALAQGALRVLKGEEQPRQYA"
     gene            36140..38263
                     /locus_tag="PLY83_00525"
     CDS             36140..38263
                     /locus_tag="PLY83_00525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOV28553.1"
                     /translation="MDGLFGEQFFNCSPLAVAVLGPGGTILKVNPAFTDLFGYTPEEA
                     EGSNLDDLIAFGPENAEARMLTRRAENGDCFDFRALRRRKGGDPVRVRCLAVRTSPGL
                     GAAVDFAIYENLERVGREADTPSEDRACLEALFGGAPCAVCIVESGSVVRQVNREFEN
                     LFGYSAEEAAGRKLDDLIVPESLREEAERLTREGDSGGTYRIETERRRKDGTLLPVMV
                     YNLKTRIGSGPVRDFVVYLDLTEAREKERLQREAADRMNRVFRAIPDAVFILDAPEGR
                     VLDMNAQAETLSGYCLEELRGKTFADLGAWLDLEERERFHRLLGEKGTVRDFEARFRR
                     KDGTEIHCLLSGERIELDGEVRIIGIGKDITERVRYEEKIRREKAYFENLFHHSPEAV
                     VLADEMSRIRRVNPAFLELFGLDPEDASPGVAVDDLIAPRDRHDEASRITREVHGGGR
                     VSTETVRYRKDGTPVEVFLQGLSFQVEGEGRAVYGIYQDIRDRKAAERKLRENAETMR
                     RLWIDTVSVLASTVDFRDPFTAEHQRNVSRVAERIAREMGLDEEAREVLSLAGLVHDI
                     GKLGIPAEILNKPCRLSPMESKLVQMHAEVGYDILKKIRFPWPLADIVRQHHERLDGS
                     GYPCGLPGEAILPEARILAVADVFDAMQSHRPYRGAFTLEQVCEELRRGAGTLFAPDA
                     VEACLKIACEARESGTDPYRGGRSR"
     gene            38260..38823
                     /locus_tag="PLY83_00530"
     CDS             38260..38823
                     /locus_tag="PLY83_00530"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HOV28554.1"
                     /translation="MKGYFDGASRGNPGEAGAGALIVDDLGKVVWETATPLGVRTNNE
                     AEYLALILLLEEADRRQIREMDVYGDSRLVVSQVSRLWKINEPRLRELASRVWTLCEG
                     KTVRFAWIPREQNSRADLLSNRALDGPGVSLEPLKWVHDGIAIASDPDGEVYAVDVRH
                     RSCSCDRFRQSGNCPHLEAAQGRGKPE"
     gene            38828..40168
                     /locus_tag="PLY83_00535"
     CDS             38828..40168
                     /locus_tag="PLY83_00535"
                     /inference="COORDINATES: protein motif:HMM:NF020416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase/BAAT C-terminal
                     domain-containing protein"
                     /protein_id="HOV28555.1"
                     /translation="MTSPVLRVFPAESLLDEDVRIRLEGVEPGSRIRLVCRLHDEKGT
                     DWVSEAEFEASAQGTVDPAEIPSLFGTYEGLDSRGLFWSMVPDPQVKGQGKFSLHDLS
                     PLTLDLEAYGQGSFLARAMLTRVLVAGGVRRVEIRENDLVGTLFLPPGPGPFPGVVDI
                     VHSGGGLRHEPFSALLASRGIAALALAPFGTPPLSRALVEIPVERVGAALKRLAAAEG
                     VDPERIGVSGTSKGAELALLAASIFPEVKAVVAFMPSSVLWSGVGRGYEKRASWTLGG
                     TPLPFVSPRYDRLAPRSKGPLHLEPMYRAALSEIPEDHPAWIPLERTSAAVLLLSPEE
                     DAVMPSALMGSQIEMRLKKAGFARPFRHRTYPGAGHLIGSDLLPGLPTTVRHTFHPLA
                     RFAIAYGGTAAQTAEAVRMSWIETLEFLREQLGSQRPVSETGPSGVRQPVPLSP"
     gene            complement(40116..40886)
                     /locus_tag="PLY83_00540"
     CDS             complement(40116..40886)
                     /locus_tag="PLY83_00540"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOV28556.1"
                     /translation="MSLGFFLYKLAGSLIVPPGLFILAAAALAAQNLRRREGRAAGFL
                     ALFLAVGLFLFSTPAGARFLAGDLEEVKASLPKAGEKAALLVLGGGVRYGGAFSGDEP
                     GPLTTLRILSAFEISRKHPWPVIVTGGSPWRAGGATTSEIMAGKLRDLGYSGPLLLVS
                     RSRTTREDLAQAREVLRAKGIRQVVLVTNAFHMKRALWIASQVMPEVLVYPFPAGHLL
                     DRVPLQPADFLPAPEMAGYLAFREKVALAAERLMAPFR"
     gene            41005..42135
                     /gene="alr"
                     /locus_tag="PLY83_00545"
     CDS             41005..42135
                     /gene="alr"
                     /locus_tag="PLY83_00545"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOV28557.1"
                     /translation="MQDLRLARVEIDISAIRENYRAVKRLLKPECRIIAVVKADAYGM
                     GLGPVARVLASEGCSHFAVATVAEAVELRSQGVGGEILVLGSIPERQAAEIPRWNLCA
                     TCGDLALATTLAQAGLLAGRPARLHVKVDTGLGRIGFAPGEILSAVKAIRSMEGTEIS
                     GIYTHFATADEPNLDYARWQGERFLDVLRALEEEGIRIPLRHACNSAGILNCPEFHLD
                     AVRCGIFLYGLPSGYASRPIGLAPVFEVKSELVAVRELPARHPVGYGLRYVTRGATRI
                     GVIPVGFNDGFLRGTRNPEVLIRGVRVPVVGSICMDQMMVDLAGVPGTRKGDEVVLIG
                     RQGGEEITALEYAGRIDSIPAQAMTLFSRRVARVYKGGEDHA"
     gene            42128..43396
                     /locus_tag="PLY83_00550"
     CDS             42128..43396
                     /locus_tag="PLY83_00550"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HOV28558.1"
                     /translation="MPEHSGILEEMAEGKAVFWKNGGKPPAREVLGRLPIGMADVLEA
                     EARLNRFAPFIRSVFPETGEGLIESPLVFVPAAKAALEKAYGKPIPGGLWVKADSELP
                     VAGSIKARGGIHEVLKVAERIALEAGLVQEGSDYSCLALPKAREVFSRKEIAVGSTGN
                     LGMSIGIMASALGFRATVTMSRDAKAWKKALLRARGVTVVEVESDYSEAVAEGRRKCE
                     ADPDCHFVDDERSIDLFMGYAVAALRLKRQLEEAGVTVDESHPLSVTLPCGVGGAPGG
                     ITFGLKSVFGDAVKCWFVEPVQACCMTLALVRGTPEVSVKDLGLDGLTEADGLAVGVP
                     SALVWGLVKDLVDGAFTLEDDELFRLLAILWDSERRKAEPSAASALMGPIVTGGTPQT
                     NHVAWLTGGLFLPEGIFEQMLSRGRELLGK"
     gene            43409..43936
                     /locus_tag="PLY83_00555"
     CDS             43409..43936
                     /locus_tag="PLY83_00555"
                     /inference="COORDINATES: protein motif:HMM:NF024094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HOV28559.1"
                     /translation="MKILVVFYSRTGVTRSIADLAASVLKELGGESVSVETEEIIDLK
                     ERSGILGYLGAGRDAALKAEARIKPPRAKPEEFDLVVIGTPVWAFTVAPAIRTFCERH
                     CRNAKAVGFFCTMGGSGDRGAFEAMEAACGKKPIASLPIVVKPGRLESMEAYRPKVRR
                     FAEQLLKACLPGFRG"
     gene            43944..44897
                     /locus_tag="PLY83_00560"
     CDS             43944..44897
                     /locus_tag="PLY83_00560"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV28560.1"
                     /translation="MKRAAVFDIGSVSVKMLVAEAKPEGGFIPLLERMRVTRLAEGLA
                     GRKEFLPEAVERTLEALAEFSAEAVALGATELEALGTEALRRVSNPGDFARRLFCRTG
                     IPLEVIEPETEARLAFRSAVQLFPDREGPACTLDVGGGSTEILSGEKERLGPRALLPI
                     GALRLKEACIPADPPGEAEISLMRGTVSKLVQRHLMGLSCTRLVGMGGTVMVLASIQA
                     GRPSFEPEKLHGTWLSRDSVDSLILKLRGLPREERMQIPGLPRDREDLALPGAVIVAE
                     VLNALGLPGLVASAWGIRHGRMAERFGQAWKGENPCPPPSD"
     gene            44876..46474
                     /locus_tag="PLY83_00565"
     CDS             44876..46474
                     /locus_tag="PLY83_00565"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HOV28561.1"
                     /translation="MSPSLGLTFFVGGLALFLYGTQVLTESFRKAFGGKTKNALASLT
                     AHRPGAFLFGVFLAGGTQSSSVATSLAVGLVDAGLLPFADALTVLMGASVGAMAVTFL
                     LGLDWTVFSPLFLAACVAGTRLPEGKVRDVSENLLGLSLLLTGMALLSAGIRPLSDSS
                     LLKGCIGAAEGNFLAVLAVSAVLTAIFQGGNAVVALSIALISAGLLPPDSVFPTALGA
                     HVGGAAPVLLVSLGTRKRNARKLAWANLLYRLSGAAAASLAWGLLPLRATLATLPPSM
                     LPPVVLGLIVVLNALLLYPFTGTLARVLEKLPIRERETLSEPLYLDEAALALPFVALR
                     LLAMEMARLAGYADELLTRILLAPEPGGRVEALRKDLPVLAESCLDFLFEIPAPPAED
                     PARMAYASISYALIALRSLVWVAAQRLAPLCLGGECGNLKARLEDSDWENSVRRLVRL
                     VGFALGSFVLGDSDLSRRALDLAEDFREDAESLRSRLLEKGGLFDSRSEVAAWEFLSA
                     AEDLVRSASEIARSESVAERFGKR"
     gene            46487..47227
                     /gene="phoU"
                     /locus_tag="PLY83_00570"
     CDS             46487..47227
                     /gene="phoU"
                     /locus_tag="PLY83_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOV28562.1"
                     /translation="MIFGSRNEEHSQDHFLYEKDRLALTALVMEMGSRAARAFRDAVR
                     ALEERDSELAQGVLEGDDVIDELEVDAELECLRSIGMRKPVREDLRFAFSVLKIVTDL
                     ERIGDEASNIAERALWLNRRPLLKPLIDIPRMRDIASTMLEQALESFEKGDPALAEVV
                     FRADDEMDRLYHSIFDELLGIMAGPAQGNPDALLSSTALLAVARHLERIGDHSVNIAE
                     RAYFMVTGRRLKKVLREAGEKPSPPFIR"
     gene            complement(47224..47682)
                     /locus_tag="PLY83_00575"
     CDS             complement(47224..47682)
                     /locus_tag="PLY83_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOV28563.1"
                     /translation="MPKRILVCLDFSRLGDDVVTYGHHLAHRLDAEVSFIHVIEFSQA
                     FRGYGNPSMISLPEAVRAKAQEHLELLVLQSEARMPTGKDHRHPLVVREGEPAEEIIA
                     FARDGRFDLVVVGNRGNSRIASLVVGSTASKVARYAPCSVLIYRPGFEPL"
     gene            complement(47695..48381)
                     /locus_tag="PLY83_00580"
     CDS             complement(47695..48381)
                     /locus_tag="PLY83_00580"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOV28564.1"
                     /translation="MKPWRERARRMIETQIRPRGIRDWRILSALERVDRTEFVPEDLA
                     PLADADGPLPIGEGQTVSQPYMVALMTELLSLPDRTRVLEIGTGSGYQAAILAEMGME
                     VVSVERIAALARAARERLARLGYRVAVFHGDGRLGVPELAPYGAVLVTAAAPRVEKAW
                     TDQLGEGGRLVVPLVIQPGLERLLVRLKTPDGFEDSWSEYCRFVPLLPGTGDDSPENN
                     GPPEECRSTE"
     gene            complement(48378..48632)
                     /locus_tag="PLY83_00585"
     CDS             complement(48378..48632)
                     /locus_tag="PLY83_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV28565.1"
                     /translation="MDSFPMRTDRESVGRLLVKVRDTFGDRIEIDILDPRCSFWIFDL
                     VRFRVKSTEPVWILDGKLLFRGIPGWTELEGVLAERLGME"
     gene            48874..49293
                     /locus_tag="PLY83_00590"
     CDS             48874..49293
                     /locus_tag="PLY83_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOV28566.1"
                     /translation="MFDLRDAFTVGMTEETSKTVEASDTVGNFSPSLNEFLSTSACVN
                     TMIRMAIDMIDRFLPEGMISVGRKVEISHTAPSLLGVHVHYRIVLTGIDENHLHFTFV
                     GWDGLGEICHGRHERVVVSREALVRKAQERAGFLTEA"
     gene            49300..50022
                     /locus_tag="PLY83_00595"
     CDS             49300..50022
                     /locus_tag="PLY83_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV28567.1"
                     /translation="MLDLSVEKRVNSNLAVASVVLSIVGILGTAIGFFGFMGALMAAS
                     PLGAVGGGGILLISLLLGVIGTVLQLVVVYQWGGALNTNLANLRLLLSHLIAHAGDET
                     ERVNYQNGLDLLAGMGVSTVLFWGYILLHLVSVFLPGGLGRLVGFVAFVFIALYLNSV
                     FRLTDAVQDLRQKLVAHVLGEGPDLPPKRIRHRNLFLSLVFMVLTLGIYWLFLLYNLT
                     AEINAYLDDDQAVRRRILQDLA"
     gene            complement(50091..51965)
                     /locus_tag="PLY83_00600"
     CDS             complement(50091..51965)
                     /locus_tag="PLY83_00600"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HOV28568.1"
                     /translation="MGGYFGTALMVDLTERTVEESLLPGEWYRDYIGGEGLGVRLLLD
                     LVDPEAELLAPEQPLIFTTGSLTGTAAPASGRCAVVFRSPLTGTLGASNAGGHFAPAL
                     KRAGYDILVVTGRSETPVVLVIDDDGVDFLDAEEAWGKGVGATEDWIRGQLEGSGWQI
                     ASIGPAGEKGVLFSSIMTDRHRAFGRGGAGALMGSKNLKAIAVRGTEELPIADPEGLK
                     EAAKAAREELFNEAFVRDEMKPYGTPSFYDAICGLGILPTRNWQRDTYPESLPEMAYK
                     TYHETLEVKPYACFGCPIACGRHTKIRTGKWAGMEGGGPEYEAMAAFGSKCEVKDLNA
                     VAAANHLANDMGLDVISTGQVIATAMEWAEKGILTKERLGGLDLAFGNGEAVVEAVRL
                     IANREGVGDLLALGSVRAAERLGPGAEGAVMAVKGMEMAADGVRASKGEAVVHAVSPR
                     GADHLRPYASTIDAFGYREPELGIAPDAAISITEDGNKGWVKPLMANAMIPNLLGTCL
                     FTNITLGIKPSTWAKLHSKAVGREMEKDELLLAAERVINLERMVNAIFGFDGSDDTLP
                     ERFLKEPGADGPGAGQVVDLKTALESFYKAMGWDPEEGLPSVETLTRLGLDWMLYGCE
                     "
     gene            complement(52071..54887)
                     /locus_tag="PLY83_00605"
     CDS             complement(52071..54887)
                     /locus_tag="PLY83_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HOV28569.1"
                     /translation="MNEDGWTFEGRKTWPPFQGEAPEKTPRERSGRIFGRGRRFWILL
                     PLLLVLFIAWPAFARFYTDWLWFDSLGFSAVFRTRILTRITLFLGSSLLFFAFLAFNW
                     ISAWKRSKDELSEAWSVRWNPRFLPWLILGAAAVLSAGNSLGGSPAWETILGFLYRGP
                     FGQKDPIFGKDIGFYVFTLPFLALIRSWLLNGLFLALGGTAVLALPGKLGRFFASDFS
                     LRDQTRRHLSILASAFVLVWGSGFLLDRWNLLYSPSGIVFGPGYTDIHALLPALSVLF
                     FLCAAAAILVLTGTGRSRKTLWWILGSLAAAVFILRSVVPGLMQSYVVKPNEFEREKP
                     YIGYHIAATLEAFGLSEVTVLDMTPENEVTPEGVAANSDTIRNIRLWDYGPLLRTYKQ
                     LQEIRSYYDFTDVDIDRYRFGGQMRQVMLSARELDPAQLQNRTWVNTHLEFTHGYGLV
                     MNPVNEVGPSGLPILFIRDLPPKVDVPLSIDRPQIYYGEKTSTYALVKTDVREFDYPM
                     GESNVRNTYEGKGGVGIGSAWRRLLFATRFGDTEIFFTGSLRPESRVLYNRNIQGALR
                     QLAPFLLYDSDPYLVVLGGKLLWVQDAYTATDRYPYSRPVSLSDPTLSNFQGANYLRN
                     SVKATVDAYDGTVTFYAIDEKDPLLAMWRSVFPSLFRPASALSPELREHLRYPEGLFE
                     IQSAVYRTYHMKDPNTFYNKEDVWTLIPEGSRKAVSPNYTILRLMGEKAPEFALIIPF
                     LPVGRNNMIAWMAGRSDGENYGKLVVYKFPKQKLIFGPAQIEALIDQNPEISAQLSLW
                     SQRGSEVIRGDLLVIPVGKGLLYVQPLYLKAERGDLPELKRVILSTGGRVAMAETFDE
                     ALRRVVGIQAGKPAGPSKPESLFPSSGPSAPGAEDLKALAQEARKAYEAAEAAQRAGD
                     WARYGEELKRLRTLLERMTGNATP"
     gene            complement(54884..56356)
                     /locus_tag="PLY83_00610"
     CDS             complement(54884..56356)
                     /locus_tag="PLY83_00610"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOV28570.1"
                     /translation="MEVRAIEITRGNWPCDFLAAGVFEDDWSTLERLETGWTERLKAL
                     AEKRAYKGKAGEVLLLPLPGELTLAMVGLGKKGPLGQETVRQAAGEIVRAALRDRGAR
                     VLFVLPDPPGWALSKAAAEGAILAGYSFEKYRGRSEDEPPRFEVETLLLADGDEAGLQ
                     TGAILGEAQNYARDLANEPGNVITPESLAETARRISEESGLECTIYDDEAIRQMGMEA
                     LWSVGKGSKNKPRLIHLAWKPGEAARERIALVGKGLTFDSGGLNIKTGDFMRTMKGDK
                     SGACAVLAAMSAVSALGLPLEVHGLIGAAENMPGGDAYRPDDILRAKNGKTIEIDNTD
                     AEGRLTLADVLCYASDLEPSVVLDIATLTGSCHIALGDYTAGLFSPDETLVGQLMEAS
                     RLSGERLWPFPMDDERLRKKLKSDVADLVNAGSRYGGAIFAAMFLEEFVKRGIAWAHI
                     DMAGTDHSKEEYGYVPKGFTGFGTRTMLEFLLSRAGRTGA"
     gene            56464..58287
                     /locus_tag="PLY83_00615"
     CDS             56464..58287
                     /locus_tag="PLY83_00615"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HOV28571.1"
                     /translation="MFGRLLFVDLASGKIQDRFLTASAVSRFLGGKGLGIALLEELAS
                     PGGDPLDFANPLCFLTGPLTGTPFPTSGRFTVATRSPLTGLWLDSHAGGHFAPFLRRA
                     GYDGIVFTGRADVPVYLWLCDGKAELREAAGFWGQGVEETVRRVREETDPQAHVASIG
                     PAGENRVRFASIAVDRDSDPWRAGMAGRGGAGAVMGSKNLKAVAVKGSKPVPLADEAG
                     LKALVKELLERVMKNPGVHSLRVLGTAALVEPMNRTGILPCFNFRKGFLENAHALNGA
                     NLRYHAKRDAGCFNCPIVCGRVMPSDGRDVKVEYEALALLGASCGVTDVADVARAIAV
                     CNDMGMDVISAGGVVAFAMDAASRGLLEEVPEFGDAAGQVRLLEAIARREGIGDLLAE
                     GSRKASEAIGVEASELAVHVKGLELPGYDPRSSWGMALAYATADRGGCHQRAWTVMPE
                     IDGAVERFSAEGTARIVKNLQDDRAAAYSLVVCDFLPMETEDALKGLKAASGIELDES
                     GYLEIGERVWNLARMFNLRHAGPEGIQDSLPQRLLREPLPMPPQGKTAVSLTVWDLKL
                     MLEDYYSLRGWDAQGHPRESTLKRLGLSEGFRESILEKGEK"
     gene            58290..59345
                     /locus_tag="PLY83_00620"
     CDS             58290..59345
                     /locus_tag="PLY83_00620"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOV28572.1"
                     /translation="MWNICLVGFGNVAQGLARILADKRERLAAKYGFGFRITSVVTAR
                     RGCLANDQGLDPAMLLEAMERDGGLWGCEGARVIDPLEAIKSPHTDILVETTPTSLVR
                     GEPGLTHIRTALKAKKHVVTSNKGPLVRAMSELTALAEGCGVAFRYEGVIMSGTPALN
                     LVRETLAGCDIQHVQGIVNGTTNFILTEMEKGKTYEEALAEAQRIGIAEADPTADVEG
                     WDAAVKACIMAVLFMDATLELEDVERTGITEVSPEMIQTALSRGKRIKLVAEVYREKG
                     RVKALVAPVAVPLTRPLAGIMGAMNAITYTTDHLGDITIVGPGAGRIETGQALLSDLL
                     AIHRVYGREEHPSRSPF"
     gene            complement(59342..60223)
                     /locus_tag="PLY83_00625"
     CDS             complement(59342..60223)
                     /locus_tag="PLY83_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOV28573.1"
                     /translation="MAESGKPVLNPVIALTVGIAAISTGAIFARVAEAPALTIAAYRV
                     GLATLVLAPFCSRKCPKELSALHHGDWLSVLGAGFFLAVHFATWIASLGYTSVASSVV
                     LVETIPLWVGILTPLVTGERPGKATLAGIALSFTGGILIGAGDFALGGRALLGDALAL
                     AGGFSGALYIMLGRRVRPRLSLLGYVTTCYGTAGVLLWILVLASRTPFTGFSAPTWWA
                     FAGMALIPQVIGHSSYNWALRWFSASTVAVCLLGEPIGSTLLAWIFFGETLTLWKTAG
                     GALILAGIVLAARGEKS"
     gene            60303..61184
                     /locus_tag="PLY83_00630"
     CDS             60303..61184
                     /locus_tag="PLY83_00630"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOV28574.1"
                     /translation="MERKRRRALAAGIAAAVSWGASFVAIKVALRTLTPLPLVWLRFG
                     LGTAVLWGFAALRREKDPLSAREWGLVVLLGFLGVPFHHLVQSTGLKTAQAATSAWII
                     SSMPVFIAILGSLFLGEKMSFRRWFGLACGACGVLLVVTRGDPGRLRFSNPGSFGDLL
                     VLLSALNWAVFSVMSRNFLKRHEPAPMMLKVMLAGWLMTTALFAVKGDLGPILRMNFQ
                     AWVSVLFLGIFCSGLAYVFWYDALKILPASQAGVLMYLNPVSATLLAVSGLGEPLSAA
                     MILGAVLITAGVWQVSR"
     gene            complement(61174..62508)
                     /locus_tag="PLY83_00635"
     CDS             complement(61174..62508)
                     /locus_tag="PLY83_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV28575.1"
                     /translation="MTVEMNPKDRALAIDIGDTYLFAMISNAPLSRAVLVKNPLDRAA
                     IDRARRSMQSEPEKFHTEMGRIASCVDATCDLVLRKCLEWQVGSIAIGTRSIKNPFSI
                     RPRALRDNPLLNLSLHLLSTLRFRCALARIDVHLVDETNTSRIDALGLEPVAGAGSRR
                     KDRATGRLTRKEYHSTTGAAIHRDINAAINIGRLVFGDSFAEPILRGRQWEHPEVEFI
                     EIEGPGPEIRSRAVSPSLTAEELDSLVAELAGIALDEARDAFLRQKLEESLAGLIHSG
                     LDFGQLDKESELQAETVRGLNTKRLRRYLKRRRDLEENARKLGLVVSRRAKKILVEWE
                     PGEEEVLQAEGEALALEAEKGGARSLLSAALSLQSRREARAARRETLEKILTDQVQAG
                     TTYRLDSPPARVRFIRTRRDTYTFPDILEVLPPSARPYLVETPARKTVPVSG"
BASE COUNT        11338 a        19144 c        20397 g        11512 t
ORIGIN      
        1 cggatgatgt tgttttcagc aggcttgtcc taaactaggg gaacgaaatc caagacaagg
       61 aggttttttc ccgtgaccat caacccctcg atcaccgatg tccttcgggc gtaccgtttc
      121 ctcaggaacc gggtccgcca tacccccgtg gaacgctcgt ttcccctgag cgaacgaacg
      181 ggggcggacg tgttcgtcaa gtgggaaaac acgcagttct gcgggtcctt caagatcagg
      241 ggggccctga acaagatgtt ttctctcacc cctgaagagc gggcccgggg ggttgtgacc
      301 ccttccagcg gcaatcacgg acagggcgtg gctttcgcgg ccgcgcaact gggggtgaag
      361 gcggtggttt gcgttcctgg agcctgtccc cggacaaagc ggcaagcgat ccagcggctg
      421 ggtggggaat gggttgagct gagagtggtt gggcaattgt atgacgacgc ggaacacgag
      481 gctcaccggc ttgcggaggc tgagggaatg accttcgttt cgtcctacga ggatgctttc
      541 gtgatcagcg gggcggccac cgtcgggttg gagctcatga tggatgagcc cgagctggat
      601 cttctgctcg taccggcggg gggcggaggt ctgatgaacg gcgtggccct ggcggccagg
      661 acgctccgcc ccgatatccg gatctggggg gtccagtcgg tggcttcgca accctgggtt
      721 gcttcctggg aagggggaag ggttgtcgag gtggaatacg gggactccct ggcggacggg
      781 ctaaccggtt ccattcctca gagcctcctg gacctggcga aaacacgcat gaaagggtgt
      841 tttgccgtca cggaggacga aatcgcccag gcgatcgcct tcctgcacgc cgaacaccac
      901 caggtggtgg agggcgccgg ggccgtcggt gttgctgcgc ttctggccgg gaaggtcccg
      961 gaggtcctct ccgggcttcg tgtcggggtg gtcatctcgg gagggaacat cgaccacgaa
     1021 aggctgatga ccgttctcga acgctgggga aagggccggg cctagttttc acccccgttg
     1081 ccgggtttca ggatctgctt gatgatctgt tcaagcagct ggtcgccgat ttgggtagtg
     1141 gggtcctcgc tggtccgggt ccccccctcg ccggtgggga tggtgatcgt gatccgcatc
     1201 tggttctgat ccgctttttc tgccggatcg gatggggacg acgggatcgg gctttccttc
     1261 cggtgctgga tgaccctcag gtcgctgatg gcgggcttcc tccagggtcc cttgatgttg
     1321 aagctgactt cctggaagtc ccggcgtgcg gctcccccaa gggctcctcc gaggaactgc
     1381 tgcaaggtgg ccgtggacca gctcccgtcg gaagaaagaa gcccttccag gccccccagg
     1441 agcaggttca gtccgcggac gttgatttct ccgtaactct tgaggtccag gctccctccc
     1501 tccccgatcg agccgtccac cgaaaggtaa cggtagaacc ggtccccggg aaccgcgttg
     1561 gcccgggttc ccggaaggag gaagaccgac ttgccgtcga tgttgaagtt ggcgttgacc
     1621 gagctgtagc ggatggaagg gcttcccgtg atgggaacga ggcttttcag ggccgagaat
     1681 ccttccactg ccccgtcctt caccttcaga agaccgtggc cgtccatgag gaatgccttg
     1741 ccgtactcgt tggaaatacg gaaatccagg tcggcctttc ccgagacggt tccctcggtc
     1801 ttgagggcgt ccttcagcgc gggggccagc tcggcccccc tgaccgtcat ccgggctccc
     1861 caccgggctt ttcccatctc cagggatccc gcggctttga cgtcgccccc gtaggctttg
     1921 gctgttccgc tctcgaccgt gatgaaatcg tccgtggtcg cgaacggggc gtcgacatcc
     1981 gtcaggtgga atccccagag caccagttcg ggggacttca ggcggccgga cccctgaaag
     2041 ccttcggcgc tgatccttcc ggaaaccctg gtgtccacgg ttcctctcag aacccccctg
     2101 gctccgctga taaaacgggg gacgaggtcc tgaagctcga gtctcgagcc ttccattgag
     2161 aaggcgagtt cgaatccctc cctgcctttg acgatcgtcc cgctaccgtt cacgggtgaa
     2221 tcgcctacca gcgcccgggc ttcccgtata tgcaggattt ccccggtgga cgccccctct
     2281 cccttcactt gaacctgcct gaaaaacagg ccagacacgt tcagttcagg aacgcgaacc
     2341 agaagcgaga ccgtcggccg gtttgcgggg cctttcaccg ttgcttcggc ggaagcgaga
     2401 cctcttatcg gcagcgccag gctgtaacgg ttcgtgattt ccttcaggtc aagcccttcg
     2461 gcggaagcct cgagatccag ctcggcaggc cgggatgcag cggccctgcg gacctggccc
     2521 gatcccttca cccggccccc gatgacttct ccctccaatt tttcgagccg gaaccggtcg
     2581 tctgctcctc ccaggctgaa atggaagcca ccgacgggaa gtcctcctgc ggtaaggctt
     2641 tccgacgagc cctccgcgct ccaggccggg gaagaaaggg cccccttcag gctgaagcgt
     2701 ccttcgaccc tgccggaaac cgggatcggg agcagcccct ccaggaacgg ggcgatctcc
     2761 attcgggaga acccgccttt caggtcggcc tccggccggg aggaaagtct ggagatccgg
     2821 ccggagagaa agagcgatgg gctccttcct gcttcagaca tggagaggat ctctaaaagg
     2881 tccgggcgga aacggaagga caggtcgaac cgccgcaagg ggaacaaggg gagcaggcgg
     2941 gcctggaccc ccgacaggtt cgcttcgaga gagagttccc ctgtagagcc ggcaacccgg
     3001 aaggaaccgc cgatctttcc ggcaagcccc agggatcgaa gggctccaaa acggtttgca
     3061 acccggaccg catccacttc catcatgcgg ccggacaggg tcaggcgcgg ttttggggca
     3121 aagagccctt ccacgagccc ggacacgctg gctttccccc cctgccagga cgccgtccct
     3181 tcctggatcc gggcgttttt cccgtcgctt gcgaaggcca ccctgagccc ctgcagggat
     3241 tcaccgagag cctcaagccg ttcggagcgg atctgtccct cgtaccgagg cgaggaagcg
     3301 ggcccgaaaa ggcggaggtc cgctcccagg atccccttgc ccttcaattc agaaagcccc
     3361 ttgaaacggg tcgccagtcg atcgaaatcg acgccctgga gtttgaggcg aaggtcgagt
     3421 tcgacccggg gcctgatttg taccggtcct ctggcttcga ccggagaatc cagccacttt
     3481 cccgaaagtt cgaggttgcc ggtgcgtcct tcctccaccg tgaaggcggc ccgggcggcc
     3541 gtgaggtcct ggcttgcgat tgacatggcc tccgcttcgg cctccacttt tccggagggg
     3601 gaccgaagcg gacccttcag gtcgatggaa accttccggg ctgttccctt caggaaaccg
     3661 atccagggca ggaccgtttt ccagcgggag atctccatgt cctctccggc aacccggagg
     3721 gtccagggca ccgggttttg cgagaggtcc gcggaaacgg tgcccgtgag aggatggccg
     3781 aaagcgttcc cggagacccc ttcgaaagag aggatcttcc cgtcgaacgc ccatcggctt
     3841 ttcaggttct cgaagtcgat cccggccacg cggccggaac ggagggatag atcccccgag
     3901 taggaaagat caggccagcg cccctccacc cgaaactccg tcaggagggg gccgaaaaag
     3961 cggtccccca gggtcggcca gaaattccgc agcgtcagga gctcgacccc ggagaggcgg
     4021 acgagaagtc cgaggttcgg gcggaccgtc ccgaagacct cgatgtgccc ttttccggcc
     4081 tccatctcga ggttttcgat ctgggcaaga cctcctacca ggcggtaccg gccccgtccc
     4141 cgggtttcga cgccgttgac ctcggcacgg aacgtcgcgt cgactgcaaa gtccgagatt
     4201 ttcagggtgg cctttcggat gtcaatcctg ccgaacggag tccgaaggag acaatcgtcg
     4261 agccggacag catcgagcgg aaggttgatt cggtcggttt cctgccggaa ttcgggaagg
     4321 atctcccgaa gacggtcgat gtcaacgtcc gcccccccga tatccaggcg ccggataaaa
     4381 atgcgccgtt gtaaaatcga gggaaggtcc tggcggaagg tcagctcggc cgcggaaagc
     4441 accggcagcc cctcccgcgt gagaacgatg tcccgggcct tatagccgat aacgggattc
     4501 ccgctcatgg agccgatgta aaggtccgca ccgagtcggc ttttcgctcc ctggacggca
     4561 aggttgcgca cgatgccttc cccgtacggc attcggactg ccgcgacggc gagcagggcg
     4621 gggatcagga tcaggaaact ccagaaggcc agtttggccg gagaacgggt tttgccgcgc
     4681 atcaggcacc tctttgaaag gaatgcgacn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnt ggccggagaa cgggttttgc cgcgcatcag gcacctcttt gaaaggaatg
     4861 cgacggttcc attttaccct accggagggg atgacagccc atgggatacg gcgggagcga
     4921 aagtccaggg cgggagcaaa aatggctgcg gctcctgggt ctgctgaagg agaagaaaaa
     4981 cgcggtcgtt ctcttttcgg ggggggtgga tagcacgctg ctcgccgccg cggcctttct
     5041 tgcgctgggg gaccgggcgg tcgccctcac cttccgttca cccctggaag acccttcgga
     5101 agcggagagg gcttcttcag cggcggaacg gatcggcatc cgccacgtct gcctggaatc
     5161 cggggacctc tcggtcccgg aaatcgcgaa caacgctccg gaacgctgtt acgcctgtcg
     5221 gaaaaaccgg gacgcgaggg cctgggcgtg ggctcgagag agtggcttca cttcggttgt
     5281 agacgggctt tccctttctg accttgcgga agaccgtccc ggaaggaaag cttcagacga
     5341 ggacgggatc ggtcaccccc tgctggaggc ctgttgggga agggaggatg tccgcgagtt
     5401 ttcccggcag ctgggaatcg agggggcgga tcgccccgga agcccctgcc tggcaacgcg
     5461 tttcccccac ggcacttttt tgagcgaaga acggctccgg caggtgggca gggcagaaag
     5521 ggccctcgcg gctctcgggt tttcccccgt ccgggtccgg tcgtttcccg gattcggggc
     5581 ggtggtggaa acgggcgacc ccgcagggat cgccgcccgg aaggaagagg tccttcgaat
     5641 cctgaaagaa gccggtttcc ctgcagggtt cgtcgacctg gaggggtacg ccagggggaa
     5701 aatgagccgc ctccccgccg acggaaacta tcccaggtaa gggcgcctcc agtcggtggg
     5761 aggaacggtc gtttccttga tcgtcctggg ggaaagccac ctcgtgatat tgttcaggaa
     5821 accggccttg tcgttcgttc ccgaatgcct ggatcccccg aagggctgcc ggccgacgat
     5881 ggcaccggtg ggcttgtcgt tgatgtagaa attcccggcc gcgaatctca aggagcaggc
     5941 cgccttctgg atggcttctc ggtcgcgggc gaagatcgac ccggtcaggg cgtagggtgt
     6001 gctggaatcg caaagctcca ggatctcgcc gtagcgttcg tccgggtaga cgaagacggt
     6061 gacgacaggg ccgaagattt cctctctcat cagcttccca tgcgggttgt tcgtgacgaa
     6121 gagggtcggt tccacgaacc acccctcttc gttcctgtag ctgcctccga agaccctttc
     6181 gtattcctcc gggcgggaga gggcgtactc gatgtagcgg acagtcttcc ggtacgcatc
     6241 ctcgtcgatg agggccccca tgaaacagcc caggtcgctg gtcggaccga cgcgaagccg
     6301 gggaatttct tccagcagga ttttccggat ctcgggccag aggctttcag ggatataggc
     6361 ccgggaaagg gcggagcact tttgtccctg gtattcgaag cctcccctga gcaggctgac
     6421 ggcgacgatc ctggggtccg ccgaagcatg gaccacggca aagtctttcc ccccggtttc
     6481 cccgacgacg cgcggaaagg accggtacag gtcgacgttt ttcccgattg tccgccagag
     6541 cgtcatgaag gtgtcgtagc tgcccgtgaa atgaaggccg gcgaaatccg ggtgttcaag
     6601 gattcggggt gcgtaccggc tggaaaacgg gacgaagttg atgacgcctg cgggcaatcc
     6661 ggcttcttcc aggactttca tgatttccca gttgcaggca agggcggcgc gggcgggctt
     6721 ccacacggca acgtttcccg ccagggccgg ggcggtcggc aggttccccg cgatgctgta
     6781 gaaattgaag gggctgaccg cgaaaacgaa tccctcgagg gggcgccagt cgaagcggtt
     6841 gatttccccc ctggcctgtt caggctgcat cccgtagagg aggtggaggt accaggcgtt
     6901 gaagcgccag aagtccacga gctcagccag gtcgatttcc gcttcgtagg gggtcttgga
     6961 caggttcagc atgatggagg cgatgtttcg cgtccgtctt ggcccagcaa ggaggtcggc
     7021 ggctttctga aaaacggctg cgcggtccct ccacggcatc gaagaccagc ttttccaggc
     7081 ctgcagggcg gattcgaccg cggaatcgat ttctttttct cctgccaggc gagcccttcc
     7141 aaggatcagg cgattgtctg ccggagagcg gatctcctgg atttctccct cccaggaacg
     7201 ccgacccccg atgaaaaggg gaatttcttc cggagctttc tgaaggcgct caagctcacc
     7261 gagaagaagt tccctttccg cggttcctgg agcgtaaaga gggatcgaat cgacgtcatt
     7321 tttgggtttt tcggccttcc acaccaggac cacacccctt tccctttatc atcatggtac
     7381 ataaaaggaa tagcaagtca acacgagcaa ccgatatagt ctttaatacg gtggaagaag
     7441 gataaggtaa tctatgcggg gttgtggata tgtggaaaaa aatgtggaaa accgccctgc
     7501 ggtctgtccc cttaggggcg agccgggtgg aaaactgaga ggatctgcaa cggatcgctg
     7561 atgggaatct ctgccccgcg ataggaggcc tggacctttc ccaaaacccg gacgcgggct
     7621 cctttcagct ccccgatcga gccgaaaagg cccggaggct cgtcgtacag gaccagggcg
     7681 gtgcgggtgc cctgttcaaa cagtcgccag ccggttttga aacgggtcat cccgtcgact
     7741 cgcatctcga gagtgatgaa ggagcccgcc aggagaggca ggtcggccca gacctgcttc
     7801 ggggtaaaga ggcgtcctgc ggcggtcccc cagaagccct ttcccccgtt gcgggcgcgg
     7861 taaagggcgg aacggatccg ccctgcgaac cggacattgg ggggaagcgt aaaaggaagc
     7921 gcaaaccctt cctcaaccag tttttcgttg accaggatcg atccgttcgg gcggtcgatc
     7981 cagacgaagg cgagaagcct gccgaaccgg tccctcggct ggacgtcggt ttcgaggcga
     8041 acccgttttc ccaggacgag gtctcggttt gcaagggcgg cttccagccc gaattcctcg
     8101 acctccctct tcgggtggtg cagctccggg gcgtcgatca tcaggtagcg caccttttcg
     8161 atgcggccgg tgtcgagcag gacctccacg gtgtccccgt cgaaaaccgt cgtcacggtc
     8221 cccaggatcg tttcgcccca ggcagtcccg agaggcaaaa gaggcccggc ggagtttccg
     8281 ggcagggtca gaaggagaag caggaaaagc aggatgaagg gcctttttgg cccgggcaag
     8341 gcggggttcc ccctttccgg cgaaaactcc cggaaatcgt gtccgatacg atatgatacc
     8401 ttgtgaggac gagtttcggg aaagggaagg agacggaagg gatgaggacg gcagaactcc
     8461 tccccctgga ggcggccctg gaggaagcac gggcgaaccg ggatcggctg tggaagtact
     8521 gcgaggccct ggctgtcggg aacacgcgcg cccggctcac cggtccggcc gaccccgaaa
     8581 ccctgtggag ggaacatgtt ctcgactgcg cggcagccct tcctctcctg ccgcaagaag
     8641 gacgggttgt cgatgtcggc acgggaggcg ggttgcccgg tgtcgtctgg gccatctgca
     8701 ggcctggcct ttccatcagc ctcctcgaca gcgtgagccg caagtgcatc gccctccagg
     8761 gaatcgtcga ttcgctcggc ctgagaaacg tgacggttgt ctgccttcgc tccgaggaac
     8821 tggccgggaa ggaccgcgag ggattcgaca tcgcggcggc cagggccgta acggccgccg
     8881 ggcctctagc ggagctgctt agcccccttg cggcggtcgg gggaaccctt ttggccttca
     8941 agggtccccg ggtccacgag gagctggatg cggttgggga gaagtggtcc cgcctggggc
     9001 ttggggatcc ggtcctctac ccgtatccgc ttgacgggaa ggagcgcgtg atcgtccgct
     9061 ggccgaaggt gaaacccacc ccggcggcat tcccgaggaa tcccgcgatg ctggaaagaa
     9121 aaccctggtg gaggtgagtc gatgctggtg atggcggtcg cgaaccagaa gggcggagtc
     9181 ggcaagacga cctgctgtgt caacctatcg gcggaactgg gacggatggg ggtccggacc
     9241 ctcgtggtgg atgccgatcc ccagggcaac agcagcagcg ctctcgggat cgaccggaac
     9301 tcccttgaac gcagcgtcta cgacgtgatc gcgggaaacc tgtccgcttc cgaagccgtc
     9361 gtggccactg cctgggaaaa cgtctttctt ctcccggcga ccctggatct ggccggagcc
     9421 gaagtggaac tggcgggggc aatgagccgc gagagtcgtc tccggcggaa gatggcggac
     9481 ctgaagggat tcgacctcgt cctgattgat tgccccccat ccctggggct tctgacggtg
     9541 aacgccctgg ttgcgagcga cggcatcctc gttcccgtcc agtgcgagta ttttgccctg
     9601 gaaggcctgg gccagctcct gcggaccatg gatctcgtgc gatcctacct cagggaggac
     9661 ctttctctct ccgggctggt catgacgatg ttcgacggtc gcacgcggct ttccagggag
     9721 gtggtggaag aggtgcgccg acagttcggg gatcttgtgc tggagacggt catcccccgg
     9781 aacgtcaagc tgtctgaagc cccgagccac gggcgaccca tcggttacta cgatcccctt
     9841 agcgcgggcg cggaagcctt cagggagctt gcaaaggagg tatcacagag atggctgggc
     9901 agaaagctct aggccgcggt ctcggggccc ttttcccggg gggcgagaat gcaacggcga
     9961 aagctgtccc ggtcagcctg ccgatcggcg aaatccggcc gaaccccgca cagccgagaa
    10021 aacggataga cccggaccag cttgagtccc tggcggaatc catccgccag cacggaatca
    10081 tccagccgct cgtggttcgc ccggccggag agggatacga gatcgttgcc ggggaaaggc
    10141 gttggagggc cgccgggttg gccgggctca aggaagtccc cgtccgcgtc ctggtgatga
    10201 acgaatccca gtcggtccag gccgccctca tcgagaacct gcagcgagag gacctgaacc
    10261 ccctcgaggt ggcggacggc atccgcgacc tgatctcccg gtttgccatt tcgcacgaag
    10321 aggcttccag gaggctgggg tggagccgtg cagcggtaac caacaagctc cggcttcttt
    10381 ctcttcccga cccggtgaag gcaatgctgg cggaccggtc tctcagcgaa ggtcacgcca
    10441 gggccctggc cgggctggac gacccggtga gggtcacaag gctggcggag cggatcgtcc
    10501 ggcaggggct atcggtccgg cagacggaaa tcctcgtcca ggaggagaag gaacggcggt
    10561 tggatccccc gccccgagag aaaaacgaga tcccgctccc tgaggatttt gccgtattct
    10621 cgaaggagaa ggggttcaag gcttccctga agcggaaagg caagggggtt tcgctcgtgc
    10681 tcgaaggcct gagccaatgg caggccgagg aacttctcga tctgatccgc accggcggag
    10741 accgggtgtt ccaggctgaa aaggggtaaa ccatgccctt caaaatggag gatctcgccg
    10801 ccttttcgaa gatcctctcc gctgccctgg tcatctgcgg cttcatcgtc ctgggactcc
    10861 tgctgggaag gcagcttgcc gggaaagggg ccccggcatg ggtggtgccg gccacgacgg
    10921 cactgggggc cttggtgggg atgtctcagg gctgggcgat gattcgaccc ctttggaaag
    10981 accgggggaa aaaacgcccg taagggaatg tttcacgtga aacagcgaag gaggaatctc
    11041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gggaaaaaac gcccgtaagg
    11161 gaatgtttca cgtgaaacag cgaaggagga atctcattgg atacgcttta ttccccatgg
    11221 cgaatggcgt atatcgaatc ggcggaaaaa acgcccggct gtattttctg cgagtttccc
    11281 aaaaaaggcg acgatgaggc cttcctgatc ctgcaccggg ggaagagctg ctttgtcatc
    11341 atgaatgcgt ttccctacaa tccaggacac ctgatggtgg ttccctacag gcacatcgct
    11401 gaatacgaga acctcctgga cgaggaactg ctcgaaatga accggctggc cggcgactgc
    11461 attgcagccc tgaagaaagc catgtcgccc cacgggttta acatgggagt caatctcggc
    11521 cggatcgcgg gcgccggggt ggaaggccat gtccatctcc acatcgttcc ccggtggagc
    11581 ggggatacca acttcatcac cgtcaccgga cagacccgtg tcttgcccga ggcgcttccc
    11641 gtgacctggg ccaaactcag ggaggcgctc cgggcccgtg aatgaaggag aggatcgttt
    11701 tctcgaaccg gccgccgaga ttgaaatcga gatgacggaa aagcggtcgc ggttcatcgc
    11761 ggcggttcgg atggcccgca cgccgccgga ggcgaaggag gcggtgaggc aaatcgcgtc
    11821 cgctcgcccg gacgccaatc accactgctg ggcctaccgg gtgggacttc cccccgtgga
    11881 gtattattcc gacgcggggg aacctgcggg gacggcggga aagccgatcc tgggggccat
    11941 ccagcgggcg gggatgacca acacggtcgt ggtggtcacg cgctactttg gggggatcaa
    12001 gctgggggtc cggggtctca tcgaagccta cggggccgcc gcgaggaagg ctctcgaggc
    12061 gtcagggacc gtttggaaag tcgcatcccg gacgtcggtg atccgccttt cctacgagga
    12121 acagggaaac ctgaggttct acctcaggga gctcggcgtc gaggccgaat ccctgaactg
    12181 ccgttacgaa gaagcggtga tcatggaggt gcccgtcccc ctttcaatgg aagaacgggc
    12241 atccgcactc ttcggctctc ttctcgcgag ggggcagatt ctcggctggg aatggcgctg
    12301 aagagcggta cccgaatcga aggaggcgaa tcgaagttga tacaggaaga aacgcaggac
    12361 tcccttaccc gaaggttgag gcatcccgtg tgccgcagcc ttcttcgcga cctgacggag
    12421 gaggagttgc ggcacctcag tgcgacggac tgcgttccga acaaccggaa tgcggctctc
    12481 tacgtcacca ggatccgctc gcggagcgcg gcttttaccg agaacgtcta cgacatcgac
    12541 gagggaacga tctccctgct ggagcaggtt tggggctacc tccgctggca gcagctgatc
    12601 cgggtaagtg cacgggtcgg atccccggag ggcagtcccc tgaaggcaaa tctctatgtc
    12661 acgaggaggt tctcccggat cgcccacatg ttttacggga acttctttcc ccaagtggac
    12721 gaagacttcg cggacatggt gaccgtggtg gtcccggaat ggcaccagag gaaggtcctg
    12781 gtcttcccga atcagcgggt gacctatatc ctgggaagcg actattacgg ggaaacgaag
    12841 atggcgaccc tccgcatggc gatgcacatc gcgagggagg aactgaacgg catcggcctt
    12901 cacgcgggga gcaagaacat ccgcctgcag tcggaaagcg gaatgaaaga actggggctt
    12961 ttggtgtttg gcctgtcggg aacgggcaag acgacgatca ccaccgcgga tcacggtttg
    13021 aaagcccctg aaggagtgga agtcctccag gacgacatca acctccttct gtcggatggc
    13081 cgttccctgg gcacggaacg gaacttctac atcaagaccg acaacgtgag caagcagccg
    13141 cccctcctgg cggcagccat ggacagccgg gcgatcctgg agaactgctg ggtcgacgat
    13201 gacggggaca tcaatttcga caaccacgcc atttccacga acggccgggc cgttgttccc
    13261 agggaggcga ttcccaacac atcgcagcgc atcgatctcc ctcataccga tatccttctt
    13321 ttcaacatgc gccgctacga tatccccccg atcggccggc tggtctcgcc agaacaggcg
    13381 gctgcctatt tcatgctcgg ggaatccacc atcaccagcg cggaggaccc cagtcgggtc
    13441 ggccaggcga aacgcgtggt cggattcgat cccttcatcg tcaaccagcc tcagaagaac
    13501 gggaaccgga ttctccagat cctgagggaa aacccccaga tccgctgcta cgtcatgagc
    13561 acgggccggg tgggagggaa cagcggtgcg aacatcaccc cggaggtcac gttcgcctgc
    13621 atcgaagcag cccttcgggg ccagctgcaa ttccgctatg acgatatcct gggctatgaa
    13681 atcccggaat cctacccctt cgagggcgga gagaggttcg acccctaccg ggtctgggac
    13741 agcgaaacct acaggaagat gatcggcgat ctcaggaagg aaaggcggga atacctgggc
    13801 aaattctcag ggctggccca ggaaatcgtt accgcggtct gagccttcgg gacggacgcg
    13861 atcggggctt tttttgcacg agattgcggt ggagtagggg aggggctata atgccatgtg
    13921 tcaaccataa cgaaaagaga ggatgacaca tgatgccaaa ccggacccgc accatgatcc
    13981 tggcttccct ctttgccgtc ctgactgcag tgggggcaca ggtgaggatt ccgcttcccc
    14041 tggttcccat gacgcttcag gttctctttg tttttctttc cggagtcctg cttccgccgc
    14101 gggccgccct gacgtcgatg atcctttacc tggccatggg cctgctcggc ctccccgtgt
    14161 ttgcgggaga aagcggaccg tcggtggttc tccaccccac cttcgggttc cttctgggat
    14221 tcattctggc ggcgtggctc atctccttga tcgtcacgct gaaagggcaa cgctttttcg
    14281 tgtgcctgac cgcctgcctg gcgggcctgg cggcgatcta cgccctgggg ctcgcggggc
    14341 tttacctgaa cgtaaacctg gtcatgggca agtcgatgac ctgggcccag gccgcgaagg
    14401 tcgggatggt tccgttcctg gcgggtgatc tcctgaaggg agtcgcggcg gccttcatcg
    14461 cctcgcgcat cggtccccga ctctccgcgg ccgggaaggg aaacgggcac tgaacaggag
    14521 gagggccccg gggaaagccc ggggcccttt tgcttgagaa cggacggaga ggatcctttt
    14581 ctcccggagg tgaaaagatg aactggcaag agcggatacg aacggattgc ccggcatgcg
    14641 ggcagcgcta cgaaacggac acccgaccca ttcggtgcgg ctgcggttcc cccctgaaca
    14701 ccacggtgga gtgggaagaa atgcgaatcg acggaagggc tcacgggttg tggcgttacg
    14761 ggtcggcgct ccctttcgaa ctcgcggagg cgccgtcgat gggtgaagga tggacgcccc
    14821 tggtccgcgc ccgcgagtcg gaaaacctgt actacaagct cgaattcttg ttccccaccg
    14881 gatccttcaa ggacaggggg gccgtcatgg tgatggcgga ggcgcgcttt cgaaaagctt
    14941 ccgaggtgat ccaggattcc tcgggcaacg ccggagcgag catcgccgcc tacgcctcaa
    15001 gaagcggaat caaggcgacg atcctggttc cggcgggcac ggcagaggga aagaaggacc
    15061 aggttttgtc ctacggggcc gaactgcgct gcgtcgacgg ggatcggacg gcgacggcca
    15121 gggaggcctg gaaactggcg caaaaccgtt tctacgccag ccacgtctgg aaccccctct
    15181 tcctcgaggg gacaaagacg ttcgcctttg aaacctggga acagctgggt ttccaggctc
    15241 cggacgtgct tttcatcccc gccgggaacg ggaccctgct cctgggagcc tggaagggct
    15301 tttcggaact cctggccagg gggctcatcg aacggttgcc ccgcctcttc gcctgccaga
    15361 gctttcggtg tgcgcccctc ttttcccgac tccacgggaa acccttcgct ggaccgggag
    15421 aaaccgtcgc tgaaggaatc gcggtttcgg ttccgccccg gattgccgag atggagcgcg
    15481 cagtcgctga aacgggggga gcctgcgtcc tcgtgaaaga tgacgaggtg ctccaggagc
    15541 agaaacgcct ggccaaagaa ggtttcctcg tagaaccgac ggctgccgtg gcctccgcgg
    15601 cagccttgaa ggcaatgcga gaacgacgct tgaaagaaga agaacggatc gtggttccgc
    15661 ttacgggctc ggggctgaaa aaaacgccgg cctaggcagg atgggttttg ggaagcttcg
    15721 tcaaaaagag gaccaggagg gggagccggg gctccccttc atttattttt ctcccgcgcg
    15781 gcatggcgtt ctaaaaggtc aaaaaaagac aacatcttct ttctccttga aaggggattt
    15841 ttccgccaaa agccgggcgc aactcagtgc ggggcggctg aaatcggttg aggcagggaa
    15901 gaacggcgga ccggccgggc aagttgaccc ggaaaaaggg agggaacccg atggctaaac
    15961 ccggaatcag gcttttttcg gctttacagg aggatggaaa ggctgatcaa tcctgaccta
    16021 acgacggaaa ccggggtaca atgaagcagg aaaccgatct gtatccaggg gaggcgagcc
    16081 ggcatgaagg acgtcgaaac gctggcggaa tggattttgg aagcaagacc gttcgtcgtg
    16141 ctctcgggag ctggaatgag caccgagtca aaccttccgg atttccgttc caggcagggg
    16201 ctttgggcgc aatatgatcc agcccggctg gcgacggagt gggccatgga aaaccggccc
    16261 gacgagttct acgcctttta tcgaatgagg gtggaagccc tgaaggcatc ctccccgaac
    16321 cggggacacc ggatcctggc ggattgggag aggcggggcc tgctgtcggc gattgtgacc
    16381 cagaatgtgg atggtctcca ccaggaggca ggttcccgta gcgtggtgga gctccatggg
    16441 agccttcgaa cggcacggtg cagggggtgc ggggcgaaga gccccggtgt gagactcctg
    16501 gaagggccct tctgccctgc ctgcgggggc cgcctaaggc cggacgtggt tcttttcggg
    16561 gaaaaccttc ccgaggctcc cctggaagaa gccgaacgcc tggcttccac ggctcggttg
    16621 ttcgtggtcc tgggctcctc cctggccgtt tctccggcga atcgcctccc gggcatcgcc
    16681 gcggcctgcg gggcgaaggt ggcgatcgtg aaccgcgaag ccacgccttt tgacggaagg
    16741 gcccacctcg tgatccagcg cggcatcgga gaagtcctcg ctgaaaccga ccggatcgtt
    16801 tcggaaaagg agtctttaaa agggccggtc taagagggag gggtcttcct ccaaaagcga
    16861 gatgaaggct gaaacgacct ccgggtcgaa atgcaccccg gctgcccgcc ggaggtggtc
    16921 gaggaccttc ccggggctcc accgtttccg gtagcaggtt tccgagagaa gggcgtccca
    16981 gacgtccacg acggcgaaaa tccttgccac gatggggatg tcctttccct tcaggccgtc
    17041 cgggtatccg ctgccgtcaa agcgttcgtg atgccgtagg gggacgtcca gggccccctg
    17101 gagggccggc acggatcgga aggcttcaaa agcccgtacc gtgtgggtcc gcaggatctt
    17161 ccagtcttcc tccgacagcg gggggctctt ctggagaagg ctgtccggaa gggtgagctt
    17221 gccgatgtcg tggagcaggg caccccggcg caggtcgatc agttcccggc tggaaaggcc
    17281 caggcgctcc ccgagcttga ccgagaggga ggtcacgcgt tcgctgtgcc cggcggtttc
    17341 gtgccctcgg agttccagca gggttgtgca cgcttccacc agggcatcga ccgaggaatc
    17401 cagcagggaa gcgccccggt cgagggcccg gaggcgttcc gttcgcgccc gtgcctcgcc
    17461 gatcgccccc gccagttcgt tcagaaagga gaaggctttc tcttccccgg aggatccgcg
    17521 gagggcggcc acgaggaacc ccgcggagag ttccgttttg tccaggggga aaaggcaagg
    17581 gcggatctct ccctgcactt tcaggaccgt ttctgcggaa ccggccggaa attccgcctc
    17641 gatccaggcg ggaggggttc cgggagcggc tgccctgcag gacaggaggg tcttctcctg
    17701 caccgggaga aagagggaaa cgggaagctg gatcaccctt gtaacgcctt ctaccagcgc
    17761 ggcgaggtct tccccggaag ggtccgactg ggtaagcacc ttccccagcg ccaggagggg
    17821 aaggggcgct tcatcgccgg gcgcggatgg agccgcctcg atcgagaacc cggcatcctt
    17881 cagaagctgc aggaaatgcg cctgttccga aagtgaagcc tcctggagcg tgacagcgat
    17941 ttttccggcc aacgcggttc cctccctgtg cggttcaaat gcagctgaag cgtgaacgat
    18001 ttctattgta tcaaagggaa cccggcgaaa gcccggtatc tgccggtacc agggaaaggc
    18061 ggggcggcaa ggctttcgtg tttgcatacc ccaggggaat ctccagaatc gatgccgcgt
    18121 ggggatggga ggcgaaggac caggggttca ggaccacgtg ccggagaacg gccccgtctt
    18181 ctcccaaaaa caggatatcc agggggtagc gcatccaatg ggaatggatg gagcggcatc
    18241 cttgaaacca gacccccatc ggcttggggt ccgtgaacat cagccccctg aagcgttccc
    18301 ggaacgtggc ggccgcccag accggctcga accaggtcct gccgtccggg ccgagcgcgg
    18361 cgagaggcac cccggaaaag agcttcgtca tggcctcagg ttctccagga tctggatcat
    18421 ggccggcccc aggatgatga cgaacaggct gggaaagatg aagaggacga gggggaaaag
    18481 gatcttcacc ggcgctttcc tcgcgtgctc ttcggctctc aggcgccgcc gcttccgcag
    18541 cccggcggcc tgggcgcgga gaaccgccgc gatgctggtc cccaggcgct cggcctggat
    18601 gatgctggcg gtgaaagatt tgatttcgtc gaagggcaac gcctcggcga tgcctctgaa
    18661 cgcgctttcg cggggagaac cgaggctgat ttccgccatg gctttcgaga aaaggtcccc
    18721 cagcggatgc cggaaccggc ttgcgacgcg ctggagggcc gcgtcgagcc ccagcccggc
    18781 ttccacgctg acgacgagca gatccattgc gtcggccagc tgggcccgag cgagttccac
    18841 tctcctccgg gtccgggagg tcacccacag gccgggcagg aggtagccgg tgcaggcggc
    18901 gaggatcgag gcgggaagga agaacctgcc ccggacgaac agtccggcgg cggtccagag
    18961 caggaaggcc gacaccacct tggacaccgc gagagctttt ccggtgatgc ccagcgggcg
    19021 ccctgccctt tcgcagtatt cgtccagccg ctcgagcgtc ctggggggat agaggcgcga
    19081 caggacgtcc accagggcct ttcccagcgg ggagaggatc ctctgcctga agctgggcgt
    19141 ctcggttctt ctctggactt ccaggtactc cgaagccctt tgaagccgga cgtcctcacc
    19201 cgggagaaaa gcctggaaga agagccagag gctcagccca agcatcgcaa acagaaccat
    19261 cgagatccac gtttccattc ggatcccctc cttcagtagc ggggcttgac gatccggttc
    19321 accaggaaaa tcccgatcac cccgaagacc ccggaaagcg tgagcatccg gactccctgg
    19381 ggagtgtgaa aaagaacgcc catgtattcc ggccgcgtca gggtgaagaa aatcccaagg
    19441 acgaccggga gcagcgagat gatccagcct tccatgcgcc cctgggccgt cagggagcgc
    19501 atttccatct gggcctcgcg ccggtcgcgc atgatcccgg aaatgttgtc cagcacctcg
    19561 gcaagattcc cgccaacttc cctctggatc aggatggcgg aaacggccag ttccaggtca
    19621 agactgcccg tgcgttccac ccacctccga agggccgttt ccacgtcgaa accgaccgtc
    19681 atctccgtga tcacctgccg gagcgtcggg ccgatcgggg cctccatctg gccggcagcg
    19741 tgctggaggc tctggaaaaa gctgaatcca gcccttaggc cggaagcgag caggtccagc
    19801 aggtcgggga tctgaccctc catttgccgt ttcctccggg cgctttggac ccgaagaaac
    19861 agcaggggaa ggctccctcc cgaggcaagg ccggcaacaa ggccgaacac tccggccgaa
    19921 aggaaccccg acagggtgca ggccaagagc aggatggccc acagaagaag ggcttcctcc
    19981 acttccaggg gaatcccggc gttctgcaaa agccgggaga ctttccgctg aagctgcctg
    20041 ggggccagcg aggaaacgaa gcgcaggcgc tggcgctcct cgttggaagg ccggtcctgg
    20101 gtgaaccagc gcttcacccg ttcgatggga tcggttcccg caaccggacg gaaggcccgg
    20161 aagacgcaca agagagaggc gagtcccagg gcaaccgaca ggagagccac aagcaggtcc
    20221 tgcaccgttc tcccccctta ccgccccgcc aggcagtccg cgggcagggc cacacccgcg
    20281 agctcgaact tggccaggtg tttcggctgg atgccggtag gccgaagttt tccgcgaacc
    20341 cgtccccttt cgtcgaatcc ctggagatca aaatggaaga ggtcctgcgt ctggatgatc
    20401 tccccttcca tgccggtgat ttccgtgacg gtcatcacgc gccgagaccc atcccgcagg
    20461 cgttcctggt agatcaagag gtccacggcg gaggcgatct ggtcccggat ggccttgagg
    20521 ggcagctcga ggcccgacat catgaccatg ctttccaggc gagccaaagc gtcccgggcg
    20581 gaattggcgt ggatcgtggt catggaaccg tcatgccccg tgttcatggc ctggagcatg
    20641 tcgaaggcct cccctccgcg gcattccccc acgatgatcc ggtccgggcg catccggaga
    20701 gcgtttttaa caaggctccg gatggggatg gcccctttcc cctcgatgtt ggccggccgc
    20761 gcttcgagcc tgacgacgtg gggctgctgg agcttgagct cggcagcatc ctcgatggtc
    20821 aggatccttt ccttttcggg gatgaagcct gaaagcacgt tcagaagcgt cgttttccca
    20881 gaccccgttc ccccgacgat cacgatgttg aaccgcgcct ccaccgcggt ccggataaac
    20941 gccacggctt cctccgacag ggtcccgccc gcgatgaggt cggcggtcgt caggggggtc
    21001 ttccggaact tgcggatggt gatcaggggg ccttccaggg agagaggggg aatgacgccg
    21061 ttgaccctcg atccgtccgg caggcgggcg tccaccatgg gggaggactc gtcaatgcgc
    21121 cttcccaggg gggagacgat gcgttcgagc acccgcttga cgtgttcgtc gtcagcgaag
    21181 acgacgtcgg tgagctcgat gttcccccgg cgctccacgt agacgatgtc cggcccgttc
    21241 accatgatct cgctgatcga atcgtcgtcg atcaaaggct gaatgggccc gtacccgaac
    21301 acctcgttca ggacttcctc gcggagccgc tcctgctcgg ctgccgacag ttccgggctg
    21361 gcggaaagaa agctgtccac gattcggacg accttcctgc gaagctcggc gttcccctgg
    21421 cttccagggt ccgacccgcc caggtccttc gggtcgactt cctcggcaag ccggaccaaa
    21481 agccgcctct gcgcggcccg gaaggccggg gaaacctgga tcctttcctt ctgttcctcc
    21541 gcggaacgcc ctgcaagccg cttgagaagg ctcatctgga tttcctccct ttcccgccgg
    21601 gggaggaatc agggaaaaga cggttcagaa gttctcccac cgctttcacg aacggcgagc
    21661 gcggaagggt cagaaccggg cttcgtccgc tttcccaggc ccgatccagg gcgggttcct
    21721 cgggaatcag ggccgaggcc ttgtaaaggg acgccatttt tgcggcagtc tgggcgtttc
    21781 ccttgttgac ccggttgatc acggcgcatc tctcttcatg gacgagccgg agctggtcca
    21841 tcaggttcca gaagaggctg acattcttga ctcccgtgag ggaggcatcc gtcacgagcc
    21901 agagacgctg cgcccgttcg gcgatgtgaa gggtgatctc gtcaaagctc ctgtgcaggt
    21961 ccgcgatgac caggtcatgg gacttctgga ggaggtccag ggcttccgac accgcctggg
    22021 ggcggatgag ttcggcctgg gccggtgatt tgggggcggc caggatcgag aagccttcct
    22081 ccgtggtcag gaggtagcgg tggatgtccc gttccccgcc gctgctgtcc cgggcccagt
    22141 ccacccaggt cagggtgggc ctcgcgttca tgaagaacgc ggtgtctccc agggcgatgt
    22201 ccccgtcggc aacagcgacc ttgagcccct tccggcgggc agccccggcc agtgccaggg
    22261 cgagggaggt cgtcccggct cccccccggc atccccaaag tccgagaagg acgcccgtcc
    22321 gatcccggtt ttgccccaca gcagcggaat aggcccggtt caccgcggca tcgagttcgg
    22381 ccacggggca ggcggcaggc acaaaatccc ttgcctggag ccggatgatg gtccggatgg
    22441 cctccggatc gttctcctcg gccgcaacga tgatccccgg gaggaactgg agggacttga
    22501 gcgcatccag gaggtccgcc gcttcctgtt ccccggcaca caatcccatg agaagaacgt
    22561 ggggcctggc ggcctgcacg agggacctca gctccgcaaa cgaaccggcc tcgccggcga
    22621 cctcgatcgt ggaactttcc tgaagtcccc tcttccaccc gtttcgacgc tcttcggtgc
    22681 ctgccaggaa aaggcggatc atggattctt caccacctgg ggaacgaccg tgatcacgag
    22741 ctcggtctcg ttgttctgga actgcttgct gcggaagagg ctgccgagaa gaggcaggtc
    22801 cgcgaggagc gggatcttct tgaccattcg ggtttgcttg ttgtccagta gccccccgat
    22861 caccagcggc tctccgtctt tcaggaccgt ccggttttct acctttcggc tccttagggc
    22921 cgggatggaa acgccgttcg tgacgatgcg gttttcccag tccagttccg atacctcggc
    22981 ggagaggttc aggaggatgt ccccatcgct gaggacttcc gggacgatcg tcatgatgac
    23041 cccgtagggt ttccattcga ctttgatctg gttgttctcg acgccgaccg gaacgggaat
    23101 ttctcctccc acgttgatga aggccttttc tccgctgagc ccgctcacgc ttggccggct
    23161 gaggatcctt gtgctggacg tgtttttcac ggcatcgaaa aggcccttga gattggacgt
    23221 gatatcggcg gcccggttcc aggtgaaccc gatctgtccc agctcggagt tgacgaggtt
    23281 cgcggagcct gaaacgagct gccccctgcc gccctcgaga taggatagcc cgagcttttg
    23341 cccgtttccc ttcgccatct ccatgacgat ggcctcgatt ttgactcgcg ggtccccctc
    23401 gacgttcagg aggttgacga cgttgggcgc gaagcccctg gcgatggcct cggccctctt
    23461 cttttcgtag ggggtccgga cggctccctc caggatcacg gtgttttccg tcaaggcgac
    23521 tttgatgccc gaatcctgca gcatctgttc aaggtcccag gtccgaactc gccgggaggg
    23581 atggacgagg atgtcgaaca cgtggcatcc ctttgaatcc cagaccacga gggaagacac
    23641 gccctcggat ttcccaaaga ggagaaggtc gttccggttg acgggttgcg cgtccaggac
    23701 gttcgggttc ccaatggcca cccgggccag ggactggaac gtgagcgggg cgttttcccc
    23761 cgtttccagg tggagggtct gttgcggttg ggcaggccct ccggcggaag gcaggggctg
    23821 ggacaacagc agaaggaccg tcgctagaac gagaacagga aaggtccgct tttttctcat
    23881 cgttcagtcc tcctcattgc actggaaccg ccacggattc cgtctttccc ggaaccatca
    23941 ccgtgacggc ccagcctcgg gtagggcgcc cggcggaggt ttcaggatgc ggtgaagaaa
    24001 cgggtttcga cggtgactgc gcctgcgcgg tgtccccgcc cggcctgggc tgctccttcc
    24061 acgcggcggg gtccggcccg gcatagaccg cttcgggggt cttccccgtg tcccctgcgg
    24121 gacgaaggga aagccagagg tccccgcggc gctgggccca ggccaaaatc ggggcctgct
    24181 gcggcgaaac ctcgaggacc accgatttcg ccagctcggc gggggccgcc ttttcccgct
    24241 cttccgcggg aatgcctgca tccaccccga cggccaggac cttcagtcca gacagcaggt
    24301 tgcgggctgg ggtgatcccg cctgggcgct tttcgaggat gaccacgtcc acccggtcat
    24361 cggtgcggat atgcccggaa accaggctga cttcgtccac ggccatggcg atcgcccgca
    24421 tcccgttcgg cagggtcagg gacaggcttc ctttcccccc tgcttcggcc agcttgaatt
    24481 tcaggatggg atctcccttg gcgaggcggt agagggtccg tttgccggcg agctggtcgg
    24541 cggccgggat ttcttccgcc gggaaggccc ctcggggcat cttcaggacc acgatgtcct
    24601 ccggggtgag ggtgctgaag gcctcgatct cccgggcggc cgccggaacc ggaatcatcc
    24661 tgttctcggc ggcgaccagg cgtttccggt actcccggat ctgcccgaag accagcgcgg
    24721 ctgcgatcaa agccagaagg agcgatagag tcaaaagcag gattttcctt cgcattccat
    24781 tccctcctca gggttccggg acgaccaggc ggatcccgta ggctccgtag tcggggacgt
    24841 tcctcgcggt cctggaattc ggggtcgcca tcttcacgaa gcgggcatag atgtccacgt
    24901 cgttgcccgt tcccaccgcg ttttccagga agaccctggc gaagccgatg acgtggacca
    24961 gctcccggcc gttgatatcg gccatggact cgatgacggg gatcgtgatc agccggctgc
    25021 tcgtctcgat gcccggcatg gactggtaat agtcccagtc gtgggaatcc tgccccacga
    25081 gatagttcac cccttcccgg gtgggtccgg ccatgttgcc cggctcggtg tagatccagt
    25141 cgtcatccgt ggagatctcg cttccgatgc ggatccagcc gtcggaaccg tacttgatgt
    25201 tctggagata gtctttggct ccgcggtccg tcgggtccag ggccaccgcg ttgaagttgc
    25261 cgtgataggc cccgcttcca gggcctggac cgtacttgat cacgtacgtc acgccgtagg
    25321 tgaaccctcc ctcgggagga acgatggcga aaggccgtag tccctggacc tcggaaacgg
    25381 gggaagcgat ggcttccgcg tgggcccgga ccaggatcgt gtcttttccc aggaaaacgg
    25441 cgaagaacgt ccggagggct ttttccccgt ccacgtggca ccggttggat gccgggaaac
    25501 ggaccgaata ggaagcctcg aggttttcgt tgcccatttg gacggcccga tcggcggctg
    25561 tttggggatg tcccatggcc agctccatgg ccccgctgag ggcagcggca tcgacggctc
    25621 tctggaggcg ctccctcatc aggaccgcgt aaccgtaatc caccgccagc gaagccgccg
    25681 aaagcaggat gcccagggaa agcgccgtca gcacaaggat gccgccgccc tgtttttggc
    25741 ggaattgacc catgatcacc cttcctttcg gaaagcggcc accgccgtgg gccggaaggt
    25801 tcccaaaaga gcgggaacga actggccgag cggtgtgacc gtcctcaggg gtaccgagat
    25861 ggagactttc gattccacgt aggaagagag ggtcacttcc tccacccgga tggtcggagt
    25921 cccggcgaaa gaccgcaggg agaggagatt caggacggcg gcggtcacct cctcggggga
    25981 ttcaccgact gccgcgaggc gagccccctc ccgggctgca tgctgaacga cctggaactg
    26041 ctggaagagg gaggccatct cgatgatccc catgacgagg accagcagca cgggaagggt
    26101 gagggccagt tcgaccaggg cggctccgcg atgtttttcc ccgttcatgc ggtgaccctc
    26161 ccgaggagag ggaaaaaatg ggaaaggatg aaaccggccg tcatcgccag ggcgtaagga
    26221 accttttccc gtttcctcga gcctgcgttc ctgcaaaggg tacggaccgc ctctttccat
    26281 ccggaggcgg cctccagcca tgcctgcgtc cagtcctccg gcctgagggc cagcacgacg
    26341 accagaacgc ctcccgacag ggcggaccag aggagggtat ccgccgcgga gggccagccc
    26401 acggcggctc ccagggccgc catgaacttg acgtctcccg ctcccatgcc gccgagccag
    26461 taaggaagga gaagggccga aaacccggca gcggctccca gggccgatga gaccaaatcc
    26521 ccgcggtacg cgtgccaggc aagggctccc agcaggatcg ccccgttcag caggttggga
    26581 actttccgga accggagatc ccaaagccct ccgaaaacgg acgccggaag aaagatccag
    26641 aaagccgcca tgttctcacc tcttccaggg ttggctttcg ccttccgggg agaaaaaagg
    26701 ggcggaggcc cgcccctttc tctcgcctgc ttcgcgcctg ggttttgctc aggacccgct
    26761 gctgttgctg ctcatctgat cggaggcatt ttgaaacacc ccggaaatgc ctccccgaag
    26821 ggttaccagg gcggccacaa gagccaccgc gatgacggcg atgatcaggc cgtactccac
    26881 catcccctgt ccctcttcat ctcgaaccag accgatcagc ttcttcatca tctcgtccac
    26941 ctcccagact gaatgtcctc tccccgattt ctaacggaaa ccccatcctg cctcgctgac
    27001 tgaaaggaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng aaatgacctg
    27121 gaaggtccgg tcgttcggcc tctttttttc gatttcaggg atttttcccg ggttcggatt
    27181 cgatcatttc caggaaagcc cgcaccacga gggggtcgaa caggcgtccc gaaccctccc
    27241 ggatgtaggc cagggccctc tcgggagtcc aggccttccg gtaaggctgg tccgccgtca
    27301 ggctgaccca gacgtccacg acggagaaga cccgggtgaa gaagggaatc ccctctcccc
    27361 tcaggccgag aggatacccc tggccgtccc agcgttcgtg gtgactgtac gggatagcga
    27421 cggaatcctt cagaaagccg atgggctgca gcatctggag ggcatagacc ggatgcaggc
    27481 gcatgacctt ccactcttcc tcggtcaggg ggccgggttt cgtgaggatc gcgtccggaa
    27541 ttcccatctt cccgatatcg tgcaggatcg cccccctgcg gatcgcctgg agatccttcc
    27601 cttccatccc gagccgccgg gcaaggcaga cggtcagctc ggcgattttc ttcgtgtgcc
    27661 attctggttc gcggcacttc aggtccaggg ctctcgacca cccttcgatg gtggaatcgt
    27721 aggcgaggtt gagttcgagg ttcgcccgtt ccagctcttc caccaggaaa ccgcggtgca
    27781 gggccgcgga agcctgcctc gcgaccgttt cggtcatttc cttcagggcc tcgggggctt
    27841 ccaggctctt cctgctgtag atctcgagga cgccgagtag ccggtcggga gccagtaggg
    27901 gtgccgcgca ggccgccctg aacccctctt cgttcaggag gaaaggctcc accctggtga
    27961 cgccggggtt gtccaggtcg ggcaggagga cggtttgcct tcgcagggcc gcctcgccgc
    28021 cgactccctc cccaacgtca gagaaccggg actggatcgc tgatccccaa aacccctccc
    28081 cttctgcgaa tttcagtttc atcgtcgcct cgtccagcag ggacacggca gccgcgtcga
    28141 cgtctgcgtg tgaaagaatc aggttgacaa ggaccttcag ggtggaccgg ggattccagg
    28201 tttcgttcaa ggccaggttc agcgattgca agaccgccag ctttcgggac cgtgcctcca
    28261 ggcatccctc ccactgtcgg gttctccctt ccggggccaa ggtggcaagg atccgggccc
    28321 ggtcgagggc atcgcgaaaa accggggaga aggaggcctc ggcagggacc tctcctcttg
    28381 cgggaaggga gaaacaaaga tctctccatt cggtttcccg ttcccccaaa aggaggccgg
    28441 taaaaaggcc ggacagcgag cgggcctgct cctggccgaa gaggctgacc aggtcgagtc
    28501 cctccagttc ctcgcgcctt gctctcaccc gttcgcagaa agcgaggttg cagctttcca
    28561 gtccgtaaac cgggtggaag agggcgacgg gcaggctggt ttggaggagg atctcaggca
    28621 ggctgcctcg ggcctcgatc gtctcggcgg aaagccgccg gagggcctct gccaggacgg
    28681 agggggacgc gtcgtaaagg aattgcctcg ctcctctctc ccgggcgacg gcagccaggg
    28741 cttcatgggc cggatcgagg acggcaaccc gtgggccggc cggcaaaaat cgagccttca
    28801 ccggccacgg ggcccggagg tccaggatca cggcatccca ccgtgcgctt tccggcccgg
    28861 ggagttcagt ccccacctcc caggcgtgcc cctcgatccc ttctgcccgg agagaccgga
    28921 gaaggggttc tgcgcccggg acagaccgcg aggcgaccag gacggccatc ggcttactcc
    28981 acccagcgtt cgatggatga agccaatcgc tgcgtcagat gcctcgttac aggtccgggc
    29041 cgcccgtccc cgacccgggt tgttccaatc cggatcacgg gaacgatctc cttgacgctg
    29101 ccggtgataa aaaactcgcg ggcccggggc agctcctcca gcctggggca ccgttcctcg
    29161 atccggtaac cggcttccct ggccacctcc aggacgaacg cccgggtgac gccggagaga
    29221 acccgctcca gcggggccgt gactagcttc ccatcgataa ccatgaaggc gttgctcgaa
    29281 gtggcttccg tgatctcgcc gccgggacag taaaggatct cgaaggcctc cgggtgttgt
    29341 cccctttcca cgaaggcggc catgtagttg atgcttttca ggagcggaag aggccgttcg
    29401 tgtggcaggg gccagagggt cacgccctcg gccgtcgcgg aggggtcgat ccggtgcatg
    29461 ggctcgaaca ggacgaacca cctcggggag ggaaatctac ccccctcgca gcggtctcct
    29521 cccgtgacgt agatccggat cgaggtttcc tcttccatga aggaaatgcc ctctcggacg
    29581 accctctcga tctcctctct cgaagccggg ggcgcgatgc cggcttccct cagggagtgc
    29641 tcgagtcggt ccaggtgcaa ggagagtgca agaggtcttt tcccaagggt gcgcagggaa
    29701 tcgaacaccc cgaccccgcg ctggagggcc aggtcgctta ctgggatgca gacggagttc
    29761 aaggggcgga acttcccttc aaaatacgcc agtgacaaaa ggaaaccctt ctttccggca
    29821 ggaggatggg ggcagactgc ccgcgccagt atacggcgaa aacgtgaaaa gacggtgacc
    29881 gctttcggtt tcttgcgccc cggcctgtcc tgtgctagtt ttcgacatat ccgccaacgg
    29941 tgaagcgggg gattttcgtg gaattcattg cgcagggatt ttcccaggcg gtgacgttgc
    30001 tcttaagcgg ggaccgggaa acctggtcgg ccatcgaagt ttcaatgcgc cttacctctc
    30061 tttcgatgct ggccagtctt ctgctgggtc ttccgttggg gatcctgctc gggacgcgga
    30121 atttccccgg caaggggtgg gtccgggtcg tggtcgacac ctgcctggct cttcccacgg
    30181 ttgtggtggg ccttttggtc tatgccctca tctccaggca ggggcccctg ggttctctga
    30241 acctgctttt cacggtccgc gggatggcca tcggccagac cttcctggca ctcccgatcg
    30301 tcgtggccct taccgcgtcc gcgctggaag gggccgaccc ccggatgctg ctgacgctcc
    30361 agacccttgg gacacctccc gggaaaatga tcctggctct cctccgggaa gcccgctatt
    30421 ccgtccttgc agcggccgtt acggcgtacg gccgcgtcgt ggcggaggtc ggggtttcga
    30481 tgatgctcgg cgggaacatc aagtggcaca cccggacgat cacgacggcc atcaccctgg
    30541 aaaccggcaa gggagagttc tccatgggga ttgccctcgg gatggtcctt cttttcattt
    30601 ctttcgcgct gaacggcgcc ctctcccttt tcaggaggag gtcggcctca tgagcgcccc
    30661 ggtctaccgc ctcgaaaacc tgcaggttcg ctaccaggac cgcctggtgc tggatgtgga
    30721 gcacctggag atcggcgagg gctcgatcct gggtctggag gggcccaacg gcagcggcaa
    30781 gagcaccctt ctgagggtcc tggctttcct ggaggctcct tcaagcggtt cgctgcattt
    30841 catgggctcc ccggccgaca gctgcgactt gctggcgctc cgccggcagg tcacgctttt
    30901 gctgcaggat ccctacctgt tgcggacctc ggttttcgaa aacatcgcct atggccttaa
    30961 ggcccgggga atccgggaag ggctgaagga cagggtgttt cacagcctcg aactcgtggg
    31021 gctcagcccg ggtgtcttcg cgggacgttc ctggaaatcc ctttcaggag gcgaagcccg
    31081 ccgcgtggcc ctggcttccc ggcttgccct ccggaccccg gtgctgcttc tcgacgaacc
    31141 cacggccggg gtggatgccg atagcgcgat tctcctcaag tccgcggcgc tgaaggcccg
    31201 ggaggagtgg ggcgccaccc tggtggtggt gagccacgac atggcctgga tgagccaggt
    31261 ggccgacagg cttctggcgt tccaggacgg ccgtccgggc cgttacgtcg tgcaaaaccg
    31321 gttcgagggg ccctggttcg agcaggaggg gggcctggtg tcgatgaaaa tcggagatgg
    31381 gcagtccatt gtcgcgacga agccccccgc ttcggggagg accggtttcc tggacgcgga
    31441 agacatcacc gttgccgttt tgaagccgca agggctctcc acgcgcaacg ccgtccgggt
    31501 gaggatcctc cagttaagcc tcgagaacgg atcgggggcc gtgctcctga agggagcctg
    31561 cggtgaccgc atcctgaccg cgcggctgac ccgctcgtcc gccgctgaac tcggcctggt
    31621 tccggggacg gaggcctggt tcctgttcaa ggcttcagct tttcaatggg aatgactgtt
    31681 ttcccgaagt gatataatcg tttttcggaa aaggcctgta aaggctttgt ttttttgtgt
    31741 gcccggacac gggtttcggt ccggtgaaag agattcctgg agggggtctg cccggtgaag
    31801 agatcctttg ttgaaggaac gtccgtttgt ttcagggcgg ctggccggca agaggaccgc
    31861 ggggtgcgct gacatgggct tccaggtcct ggccatcaac cctggctcta cgagcaccaa
    31921 ggtgggttgg ttcgatgacg gcgtcgaagt ctggcacagt acggtaaccc acgatcccga
    31981 cgagctggcg gcctttccga ccatggccga ccagttcggg ttccggatgg ccgccatcga
    32041 gaaagcctgc gaggaacacg gcaaccattt cagccagctt gccgcggttg tgggaagagg
    32101 cggccttctg gatccgatcc cgggcggaac ctacgccgtg gacgctctca tgaaggttca
    32161 tctccggcgc gggaaaccct gggagcacgc ttcaaacctg gggggcatcc tggccgacgc
    32221 gattgcggcg ccgctcggga tcccggccct gatcgtggac ccggtggccg tggacgagct
    32281 ggatgacgtg gcccgcgtga ccgggcttcc cgagctgccc aagagatccc tggggcatgc
    32341 cctcaacatc aaggcgacgg tacgccgggc ggcgagggac ctgaacggcg actggaaaaa
    32401 gatggatttc gtcgtggctc acctgggagg cggcatcacg gtttgcgccc atcgccaggg
    32461 aaggatggtc gataccaaca acgccaacga gttcggcccc ttctcccccg agcgcgcggg
    32521 cggcctgccg gccggagacc tcgcccgcct ctgcttcagc ggccgctaca ccctgcagga
    32581 cctgagaaaa cgcctggtcg gcaagggagg gctcgtggcg tacacggggg aaagcgacgt
    32641 gcgaaaggtc aaggccctca ttgccggcgg agacgagtcc gcggccttgg cctaccgctc
    32701 catggccttt caggtggcca aggaaatcgg cgcctgtgcc gcggcgatgg ggggaaaggt
    32761 cgatgccatc ctcctgacgg gaggagtcgc tcacgattcc gatttcgtcg agatggtggt
    32821 cgggagggtg cagtggatcg ccccggtcct gctgtatccc ggcgaggatg aactgctcgc
    32881 cctggcggaa ggagcgatgc gggttctcag gaaagaggaa accccgatgc gttacgggga
    32941 tttcgtgaag acggaggcct gaagagcgat gcagcttaaa aacctggatt tcctgctgga
    33001 aaaatgcaaa gccgcccccc ggatgactct cgccgtggcc tgcccccagg gggatgacat
    33061 cctggaagcg gtagcggacg cagcgcaaga gggagtcgtc gatcccgtcc tggtgggcaa
    33121 ccgcgggaaa acgcttgcca ttgccgaaag cctcggcctg gacatttccg ggcttgagtt
    33181 cgtcgacgag ccggacgatg cccttgccgc ggaaaaggcc gtccggatgg tgtcctcggg
    33241 agaagcgcac ctgctgatga aaggcaacgt caaaaccgcg gttctcctga aggccgtcct
    33301 gaacaaggag tgggggcttc gcagcgggtc cttgctttcc cacctgttcc ttttccaggt
    33361 ccctcgcatg cccggggtga tcgggatgag cgacgggggg atgacgatgt accccgacct
    33421 tccgaccaag gttggaatcg tggaaaatgc agtctgggcc taccaccgga tcggagtgga
    33481 gtgccccaag atcgcggccc tggcggccgt ggaagtcgtg aacccggaca tgcaggccac
    33541 gctggacgcg gcggccctgg cccagatgaa ccggcggggg cagatcaagg ggtgtctcat
    33601 cgacgggccc cttgccctgg acaacgccgt cagcgccgag gcagcccgga tcaaggggat
    33661 tgaatctccc gttgccggcg acgcggacat gctccttgtc ccgagcatcg aggccggcaa
    33721 tttcgtgggg aaagtcatga tgtacatgtc gggaggcaag ggggccgggg tcatccttgg
    33781 agctcgccgt cccatcgttc tcacgagccg gttcgacaac gcggaaacca agcggctttc
    33841 gatcgctttc ggagccgttc ttgccatgtc ctgaagcgag tcaaaattat cttttcaagg
    33901 aggtttcaac gtatggaaca gcttcgttcg ctcaccgcac tgctggatta cgcaaaacgg
    33961 atcggcgccg agaaggggcc ccgcaaggtc gccatcgctc tcgcggagga cgcgggggtc
    34021 cttgaggcca tcgaggcggc ccgccgggaa gggttcgcct tcggcatcct cgtgggcaac
    34081 agcgacaaga tcaaggctga ggccgacaag gcgggaatcg acctggccaa ctacgaggtc
    34141 gtcgacgagc gccgcgggga ggcccaggtg ggcctgacgg ccgtccagct cgtttcctcc
    34201 ggcaaggccg acatcttcat gaaaggcatg atccacacca actacttcct caaggccctt
    34261 ctgaacaagg agatcgggct ccgcaccggc aaaaccattt cccattgcta cttccatgaa
    34321 gtgaaggggt acgaccgcat cttcttcatt gccgatgccg ccttcaacat gtaccccgac
    34381 ctcaagacca aggccgacat cctcgtgaac acggtcgggc ttgcccgttc cttcggggtc
    34441 gagtgcccga aggtcgctgt cctcgctgcc gttgaggtcg tgaaccccga catgccggcg
    34501 acgctggatg ccgccgccat tacgcagatg cagcgccgcg ggcagatcaa gaactgcctc
    34561 gtggatggcc cctttgccct ggacaacgcc gtcagcgagg aatcggccaa acacaagggc
    34621 atcgtttccg aggtggcagg aaaggccgac atcctgctcg tcccgaacat cgacgccggc
    34681 aacatgatgg tcaaggccct ggtctacttc tccgagaaca agaccgcggg actgatcctg
    34741 ggcgcctcgg ccccggtggt cctgacgagc cgttccgaca cgatcgaggc caagacgctg
    34801 tccatcgctg cagccgtcgc cctctcggtc ttcaagggct aggagagttc agccgagggg
    34861 gctgcctagg gaggccccct tcaccgatgg gaggagtagc tttggagaag ggaaaatacc
    34921 ggatcctgac gctgaacccg ggttccacca gcacgaaggt ggcgcttttc gaggacgaaa
    34981 ccgagctctg gagcgatacc cagcggtacg acgcggacct cgtccggacc tttgcgaaca
    35041 tcatggacca ggaatccttc cggctggatg cggtgcggca ggccctggcg cgccacgaaa
    35101 cgccggtttc gagcctcgat gccgtcgtcg gccggggggg gcttctgaag cccatcccgg
    35161 gcggaacgta taccgtgagc cccgccatga tcgaggacct gaagagctgc cgctggggag
    35221 cgcacgcttc gaacctcggg gccccgatgg cagcgcgcct ggcggcggaa gcggggcacg
    35281 agcgggcctt catcgtcgat cccgtggtgg tcgacgagct ggtccctgag gcacggctct
    35341 cgggcatccc cgagatcgaa cggcgatcga tcttccacgc gctgaaccag aaggcggtgg
    35401 cccgccgtgc cgctcgtgac ctgggaaagg cctacgcgga agtcaacctc gtcatcgccc
    35461 acatgggcgg ggggatttcc gtcggcgccc acgagagagg gcgcgtgatc gacgtgaaca
    35521 acgccctgga cggggaaggg ccgttttcac cggaaagggc cggctccctg cccgccggag
    35581 agctcgttcg cctctgtttc tccgggacct acggcctgga ccagatcatg aaaaagctgg
    35641 tgggaggcgg cgggctggtg gcacacctgg gaaccaacga cctgcgggaa gtcgagcggc
    35701 gcatcgacga aggcgatgag aaggcagggc gtgtctatga ggcaatggcc taccaggtgg
    35761 ccagggaaat cggggcaagg gccgcggtcc tgaaagggca ggtcgatgcc gtcgtcctga
    35821 cggggggcct ggcctacagc agccggttca ccgaccggat cgcctcccgc gtggccttca
    35881 tcgccccggt ccggatctac cccggcgagg acgaatcggc ggccctggcc cagggagccc
    35941 ttcgcgttct caagggggaa gaacagccgc ggcagtacgc gtaggttcaa ccaaaggaaa
    36001 gctcgggaag gcccggcgaa ccccgcaagg gtgccgggcc ttttttcgcg gcccgttccc
    36061 ggtgcttctc ctgcgcttcg aagagggtgc tacaatcaac acggcatcag ccgagcagga
    36121 gatcggaggg gaaaaagcga tggacggttt gttcggagaa cagttcttca attgttcccc
    36181 ccttgcggta gcggtccttg ggcctggcgg aacgatcctg aaggtgaatc ccgcttttac
    36241 ggatcttttc ggctatacgc cggaagaggc cgagggatcg aacctggacg acctgatcgc
    36301 cttcgggcct gaaaacgccg aagccaggat gctcacccgc cgggcggaaa acggggattg
    36361 tttcgatttc cgggcgctcc ggcggcgcaa gggcggggac cctgtccgcg tccgctgcct
    36421 tgcggtcagg acgtccccgg gattgggagc agcggtcgac ttcgcgatct atgaaaacct
    36481 ggaaagggtg ggtcgggagg cggacactcc ctcggaagac cgggcctgcc tggaagccct
    36541 gtttggggga gccccctgcg cggtgtgcat cgtggaatcg ggaagcgtcg ttcggcaggt
    36601 caaccgggaa tttgaaaacc tgttcggcta ttcggcggaa gaggctgcag ggagaaagct
    36661 cgacgacctg atcgtccccg aatccctcag ggaagaggcc gaacgcctga cgcgcgaggg
    36721 ggattccggc gggacgtacc ggatcgaaac cgaacgaaga cggaaggacg ggacgctcct
    36781 tccggtgatg gtctacaacc tgaagacgcg gatcgggagc ggccccgtgc gtgattttgt
    36841 cgtgtacctg gacctgacgg aagcgcggga aaaggaacgc ctccagaggg aggcggccga
    36901 ccggatgaac cgggtcttcc gggccatccc cgacgccgtc ttcatcctgg acgccccgga
    36961 ggggagagtc ctggacatga acgcccaggc ggaaaccctt tccggctatt gcctggaaga
    37021 actccggggg aagacgttcg ccgacctggg ggcctggctg gacctcgagg aaagggagcg
    37081 tttccaccgg ttgctggggg aaaagggaac ggtgcgggac ttcgaagccc ggttccggag
    37141 aaaggacggg acggaaatcc actgcctgct ttccggggaa cggatcgagc tcgacggtga
    37201 agttcggatc atcggcatcg gaaaggacat caccgagaga gtccggtacg aggagaaaat
    37261 ccggagggaa aaggcctact tcgagaatct tttccaccac tctccggagg ccgtggtgct
    37321 ggcggacgag atgagccgga tccgccgggt gaaccccgcc ttccttgaac tctttggcct
    37381 ggatccggag gacgcctccc ccggggtcgc ggtggacgac ctgatcgccc cccgggaccg
    37441 gcacgacgag gcctcccgca tcacccggga ggtccacggg ggcggccggg tcagcacgga
    37501 aaccgtgcgt taccggaagg acggcacgcc cgtagaggtg ttcctccagg ggctgtcctt
    37561 ccaggtggaa ggcgaggggc gtgccgtcta cgggatctac caggatatcc gcgaccggaa
    37621 agccgcggag cggaagcttc gcgagaacgc cgaaacgatg cgccgcctct ggatcgatac
    37681 cgtcagcgtg ctggcctcca cggtcgattt tcgtgacccg ttcacggccg agcaccagag
    37741 gaacgtctcc cgggtcgccg agaggatcgc ccgggaaatg gggctggacg aggaagcccg
    37801 ggaagtcctt tccctggccg ggcttgtcca cgacatcggg aagctgggaa tcccggccga
    37861 gatcctcaac aagccctgcc ggctgagccc gatggaatcg aagctggtgc agatgcacgc
    37921 ggaggtcgga tacgacatcc tgaagaagat ccggttcccc tggcccctgg cggacatcgt
    37981 ccgccagcat cacgagaggc tggacgggag cggctatccc tgcggcctgc ccggtgaggc
    38041 catcctcccg gaagcccgga tcctggcggt cgcggacgtg ttcgatgcca tgcagtcaca
    38101 ccggccctac cggggagcct tcacgctgga acaggtctgc gaggaactcc gccggggcgc
    38161 cgggaccctg ttcgccccgg atgcagtcga agcctgcctg aagatcgcct gcgaggcccg
    38221 cgaaagcgga acggatccgt accggggagg gcgatctcga tgaagggata ttttgacggg
    38281 gcatcaaggg gcaacccggg cgaagccggg gccggagccc tgatcgtgga cgacctgggg
    38341 aaggtggtct gggaaacggc gaccccgctg ggtgtccgga cgaacaacga ggcggagtac
    38401 ctggccctca tcctcctgct cgaggaagcc gaccgaaggc agatccggga aatggacgtc
    38461 tacggggaca gccgcctcgt ggtcagccag gtctcacggc tctggaagat caacgaaccc
    38521 aggctccgcg agctagcgag ccgggtttgg acgctctgtg aggggaaaac ggtccggttt
    38581 gcctggatcc cccgagagca gaacagccgg gctgacttgc tgtccaacag ggccctggac
    38641 ggacccgggg tctcgctgga acccctgaaa tgggtgcacg acgggatcgc cattgcatcg
    38701 gacccggacg gggaggtcta cgcggtcgat gtccggcacc gctcctgctc gtgcgatcgc
    38761 tttcggcagt caggcaactg cccccacctg gaagcggccc agggccgcgg gaagccggag
    38821 tgacgccgtg acgtccccgg tccttcgggt ctttccggcg gaaagccttc tggacgaaga
    38881 cgtccgtatc cggttggaag gcgtcgaacc tggatccagg atcaggcttg tctgccgcct
    38941 ccacgacgaa aagggcaccg actgggtgtc cgaagccgag ttcgaggctt ccgcccaggg
    39001 aacggtcgat ccggcggaaa tcccctctct ttttgggact tacgagggac tggattcccg
    39061 ggggcttttc tggtcgatgg tccccgaccc tcaggtcaag ggacagggaa agttctctct
    39121 tcatgacctc tccccgttga ccctggacct ggaggcctac ggccagggga gtttcctggc
    39181 ccgggcaatg ctgacgcggg ttcttgtcgc cggtggggtc cgtcgcgtgg agatccggga
    39241 gaacgacctg gtcggaaccc tgttcctccc ccccggtcca ggccctttcc cgggcgtggt
    39301 ggatatcgtc cattccggag gggggcttcg gcacgaaccg ttttccgccc ttctggcctc
    39361 acgggggatt gccgcactgg ccctggcccc gttcggcact ccgcccctct ccagggctct
    39421 cgtggagatc ccggtggaaa gggtcggggc ggccctgaag cggcttgcgg cggcagaggg
    39481 ggtcgatccg gaaagaatcg gggtgagcgg aacctccaag ggggcggagc tggccctgct
    39541 ggcggcttcc atttttcccg aggtgaaggc cgtggttgcc ttcatgccga gcagtgtcct
    39601 ctggtccgga gtgggcaggg ggtacgaaaa gagggcgtcc tggacactgg gcgggactcc
    39661 cctgcccttc gtttccccac ggtatgaccg ccttgctccg cgatcgaaag ggccccttca
    39721 cctcgaaccc atgtaccgtg cggcactttc tgaaatcccc gaagatcacc cggcctggat
    39781 tcccctggag cggacttccg cagcggttct cctgctgtct cccgaagaag acgccgtgat
    39841 gccctccgcc ctgatgggaa gccagatcga gatgcgcctg aaaaaagctg gatttgcaag
    39901 gccttttcgg catcgaacct accccggggc gggtcacctg atcggctcgg atcttctgcc
    39961 tggcctgccg accacggttc ggcacacgtt tcaccccttg gcccgttttg ccatcgccta
    40021 cggggggacc gccgcccaga ccgcggaagc cgtccggatg tcctggatcg aaaccctcga
    40081 gtttctccgg gagcagctag gatctcaacg gcctgtcagc gaaacggggc catcaggcgt
    40141 tcggcagcca gtgccacttt ctccctgaag gccaggtacc cggccatctc gggggcgggc
    40201 aggaaatccg ccggctgcag cgggacacgg tctagcaggt gcccggcggg aaaggggtag
    40261 acgaggactt caggcatgac ctgacttgcg atccagagcg cccgtttcat gtgaaacgcg
    40321 ttggtgacga gaacaacctg gcggatcccc ttcgctctga ggacctcacg ggcctgggcc
    40381 agatcttccc gggtcgtccg ggaacggctc accaggagca atggcccgga atagccgagg
    40441 tcccggagct ttcccgccat gatctctgag gtcgttgccc cgccggctct ccacggcgag
    40501 ccgccggtta cgatgacggg ccaggggtgc tttcgggaga tttcgaacgc ggagaggatc
    40561 cgaagggtgg tcaaagggcc gggctcgtct cccgagaatg ccccgccgta ccggactccc
    40621 cctcccagca cgagaagagc ggccttttct cccgctttgg gcagagacgc cttcacttcc
    40681 tccaggtcgc cggcgagaaa gcgggcgcct gccggggtcg agaaaagaaa aagcccgaca
    40741 gccaaaaaca aagccaaaaa gcccgcggcc cggccttccc gacggcgaag gttctgcgcg
    40801 gcgagcgcgg cggcggccag gatgaaaagc ccgggaggaa cgatcaacga tcctgccagc
    40861 ttgtaaagga aaaagcccag ggacatcccc ttcctctcct ctcaccgacc cactttcttt
    40921 cccattgtac cgcgacagcc tcgttgtcat agaatggaag caagggcgaa agctgagtca
    40981 cgccgggagg gatcggaaat cgaaatgcag gatttgaggc tcgcaagggt ggaaatcgac
    41041 atctctgcaa tccgggagaa ctaccgcgca gtgaaacgtc tcctgaagcc ggaatgccgg
    41101 atcattgcgg tggtcaaggc agatgcctac gggatggggc tgggtcccgt tgcccgggtc
    41161 ctcgcttccg agggctgctc tcactttgcc gtggcgaccg tcgctgaagc cgttgagctg
    41221 cggagccagg gagtgggagg agagatcctc gtcctgggtt ccatccctga gagacaggcg
    41281 gcggagatcc cccggtggaa cctctgcgcc acctgcgggg acctggctct cgcaacgacc
    41341 ctcgcccagg ccgggctgct ggcaggcagg cccgcccgcc tgcacgtgaa ggtcgacacc
    41401 ggccttgggc ggatcggttt tgcaccggga gaaatcctgt ctgccgtcaa ggcgatccgt
    41461 tccatggagg ggacggaaat ttccgggatc tacacgcact ttgcgaccgc ggacgaaccg
    41521 aacctcgatt atgcccggtg gcagggggag agattcctgg atgtcctccg cgcactcgaa
    41581 gaggaaggga tccggattcc cctccgccac gcctgcaata gcgcggggat cctgaattgc
    41641 cccgaatttc accttgacgc ggttcggtgc ggcatcttcc tgtacggcct gcccagcggc
    41701 tatgcctctc ggccgatcgg cctcgcgccc gttttcgagg tcaagagcga gctcgtggcg
    41761 gtacgcgagc ttcccgccag gcacccggtt ggatacggcc ttcggtacgt gacgagaggg
    41821 gcgacccgga tcggggtgat ccccgttggc ttcaatgacg gattcctgag ggggacccga
    41881 aacccagagg ttctcatccg gggagtccgt gtccccgtgg tgggttcgat ctgcatggac
    41941 cagatgatgg tcgacctggc cggtgtcccg gggacccgca aaggggacga ggtcgtgctc
    42001 atcggccgcc agggagggga ggaaatcacg gccctggagt acgcgggccg gatcgacagc
    42061 ataccagccc aggccatgac ccttttttcc aggagggtgg cccgggtcta caagggaggg
    42121 gaagaccatg cctgaacatt ccgggatcct tgaagagatg gcggagggca aagccgtttt
    42181 ctggaaaaac ggcggcaaac cccctgcaag ggaggttctc ggccggctgc ccatcgggat
    42241 ggccgacgtc ctggaggcag aagcccggct caaccgcttc gccccgttca tccggagcgt
    42301 tttcccggag accggggaag ggctgatcga atcccccctg gtttttgtcc cggcagcgaa
    42361 ggcagcgctg gagaaggcct acgggaaacc gattcccggc ggtctctggg tcaaggcgga
    42421 cagtgaactc cccgttgcgg gatctatcaa ggcccggggg ggcatccacg aggtcctgaa
    42481 agtcgccgag aggattgccc tggaggccgg cctcgtccag gagggatccg actattcctg
    42541 cctcgccctg ccgaaggcgc gggaggtctt ctcccgaaag gagatcgccg tcggttccac
    42601 gggcaacctg gggatgagca tcgggatcat ggcgtcagcg ctggggtttc gggcgaccgt
    42661 caccatgtcg cgggacgcca aggcctggaa gaaggccctt cttcgggcca ggggcgtcac
    42721 cgtggtggag gtcgagtcgg attacagtga agcggtggcg gaggggcgcc ggaagtgtga
    42781 agcggatccc gactgccact tcgtcgacga cgagcgttcc atcgaccttt tcatgggcta
    42841 tgccgtcgcc gctctccgcc tgaagcggca gctggaggag gccggcgtga cggtcgacga
    42901 gtcgcacccc ctgagcgtca cgctgccctg cggagtggga ggcgcgccgg gtgggatcac
    42961 cttcggcctg aagtccgtgt tcggggatgc cgtcaaatgc tggtttgtgg agccggtcca
    43021 ggcctgctgc atgacgttgg ccctcgttcg ggggaccccg gaggtttccg tgaaggacct
    43081 ggggctggac ggcctgacgg aagccgacgg cctggcggtg ggggtgccat cggccctcgt
    43141 ctggggactc gtgaaggacc tcgtggatgg ggctttcacc ctcgaggacg acgagctttt
    43201 ccggctcctg gccatcctct gggattcaga aaggcggaag gcggagcctt ccgcggcaag
    43261 cgccctgatg gggccgatcg ttacaggagg aacaccgcaa acgaaccacg tggcctggct
    43321 gaccgggggg ctcttcctgc cggaggggat tttcgaacaa atgctttcca ggggaaggga
    43381 acttcttggg aaataggagg ggattctggt gaagattctc gtggtgttct attccaggac
    43441 gggggtgacc cggtcgatcg ccgacctggc ggcttcggtt ctgaaggaac tggggggaga
    43501 gtccgtctcg gtcgaaacgg aggaaatcat cgacctcaag gaacggtcgg gcatcctcgg
    43561 ttacctgggg gcaggccgcg acgccgccct gaaggcagag gcccggatca agcctccccg
    43621 ggcgaagccg gaagagttcg atctcgtggt gatcggaacg ccggtctggg ccttcaccgt
    43681 agctcctgca atccggacct tctgtgagcg tcattgccgt aacgcaaaag ccgtcgggtt
    43741 tttctgcacg atgggaggct cgggagaccg gggggctttc gaagcgatgg aagccgcctg
    43801 cgggaaaaaa cccatcgcct cccttcccat cgtcgtgaag cctggcaggc tggaatccat
    43861 ggaggcgtac aggccaaagg tccgccgttt tgcggaacag ctgctcaaag cctgccttcc
    43921 gggctttcgg gggtaaaggg ctcgtgaaac gagccgccgt tttcgacatc ggctccgtct
    43981 cggtcaagat gctcgtggcg gaagccaagc cggagggcgg gttcatccct ctcctggaac
    44041 ggatgcgggt gacccgcctg gcggaggggc tggccggaag gaaggaattc ctgccggaag
    44101 cggttgaaag gactctcgaa gccctggccg agttttccgc ggaagccgtt gccctgggtg
    44161 caacggaact ggaagccctg ggaaccgagg ccttgcggag agtctccaac cccggggatt
    44221 ttgcccggag gctgttttgc cgaacgggaa tcccgcttga ggtcatcgag cccgaaaccg
    44281 aggcccgcct tgcctttcgt tcggccgtcc agctgtttcc cgaccgggaa ggccccgcat
    44341 gcacccttga cgtgggagga ggaagcaccg aaatcctttc cggcgagaaa gaaaggctgg
    44401 gaccgcgtgc cttgctgccc atcggagcct tgcggctgaa agaagcctgc atccctgccg
    44461 atccccccgg agaggccgaa atttctctca tgaggggaac cgtttccaag ctcgtgcagc
    44521 gccacctcat gggcctttcc tgcactcgcc ttgtcggcat gggaggaacc gtcatggtct
    44581 tggcttccat ccaggccgga cgcccctctt tcgagcccga aaagcttcat gggacctggc
    44641 tttcgcggga ttctgtcgat tcgctgatct tgaagcttcg ggggcttccc cgggaggaaa
    44701 ggatgcagat tccagggctt cctcgggacc gcgaggatct tgctcttccg ggggcggtca
    44761 tcgtcgcgga agtcctgaac gcccttggcc taccgggcct tgtggcgtct gcctggggga
    44821 ttcggcacgg acggatggcg gaacgtttcg ggcaggcatg gaagggggaa aacccgtgtc
    44881 cccctccctc ggactgacgt tcttcgtcgg gggcctggcg ctctttcttt acggaaccca
    44941 ggttctgacc gaatcgtttc gcaaagcctt cgggggcaag acgaagaatg cccttgccag
    45001 cctgacggca catcggccgg gggctttcct ctttggggtt tttctcgccg gcgggaccca
    45061 gagcagttct gttgccactt cactggctgt cggcctcgtg gacgcggggc tgctcccttt
    45121 tgcggacgcg ctgaccgtcc tcatgggagc cagtgtcggg gcgatggccg tgaccttcct
    45181 cctggggctg gactggactg ttttctctcc ccttttcctg gccgcctgcg ttgccgggac
    45241 gaggctgccg gagggaaaag tcagggacgt ttccgagaac ctgctggggc tctcgctcct
    45301 tctgacgggc atggcgctcc tcagcgccgg aatccggccc ctttccgaca gcagtctcct
    45361 caaaggctgc atcggcgcgg cggagggaaa cttcctggcg gttctggcgg tttcagcggt
    45421 cctcacggcg atctttcagg ggggcaatgc cgtggtggcc ctctcgattg ccctgatcag
    45481 cgcgggactg ctcccaccgg attccgtctt tccgaccgct ctgggggccc acgtgggggg
    45541 cgccgccccc gtccttctgg tttccctggg aacccggaag cgaaatgccc ggaagctggc
    45601 ctgggccaac ctgttgtacc ggctgtccgg ggctgcagcc gcaagcctcg cctgggggct
    45661 tctgcccctc cgcgcaaccc tggcgacact ccccccgtcc atgttgcctc ccgttgtcct
    45721 gggcttgatc gtcgtgctga acgctcttct cctgtatccc ttcaccggca ctcttgcgag
    45781 ggtcctggaa aaactcccca ttcgagagcg ggaaaccctc tcggagccgc tttacctgga
    45841 tgaggccgcc ctggctctgc ccttcgtcgc gcttcgcctt ctcgccatgg aaatggccag
    45901 gcttgcgggc tatgccgacg aactgctgac gaggatcctc ctggccccgg agccaggagg
    45961 gagggtggag gcgctgcgga aagaccttcc ggtgctggcg gaatcctgcc tggacttcct
    46021 gtttgagatt cccgccccgc cagcggagga tcccgcgaga atggcttacg ccagcatttc
    46081 ctatgccctg atcgcgctcc ggagcctggt ctgggtggcg gcccagcggc tggcacccct
    46141 ctgcctggga ggagagtgcg gcaatctcaa ggctcgcctg gaggatagcg attgggaaaa
    46201 ctcggttcgg cgcctggtga ggctggtcgg gttcgcgctc gggtcctttg tgctcgggga
    46261 ttccgacctc tcgcgaaggg ctttggacct ggcggaggat ttccgggagg atgccgaatc
    46321 gctccgctcc cgcctgctgg aaaagggagg gctgttcgat tcccgctcgg aagtcgcggc
    46381 ctgggagttc ctgtctgccg cggaagacct ggtccggtcg gcttcggaaa tcgcccggag
    46441 cgaatccgtt gccgaaaggt ttggaaagcg ctagggaggg gttcgcatga tcttcggttc
    46501 aaggaacgag gaacattctc aggatcattt cctgtacgag aaagaccgcc ttgccctgac
    46561 ggccctggtg atggagatgg gaagccgggc cgcaagggcg ttccgcgatg cagtccgggc
    46621 cctggaggaa cgcgacagcg agctcgccca gggcgtcctg gagggcgatg acgtgatcga
    46681 cgagctggaa gtggacgcgg aactggagtg cctccgctcg atcggaatgc ggaagcccgt
    46741 tcgggaggac cttcgctttg ccttttcggt cctgaagatc gtgaccgatc tcgaacggat
    46801 cggggacgag gcttcaaaca tcgccgaacg ggcgctttgg ctgaacaggc gccccctcct
    46861 gaagcccctg attgatattc cccggatgag ggacattgcc tcaacgatgc tggagcaggc
    46921 ccttgaatcg ttcgagaagg gggaccccgc cctggcggag gtggtgttca gggccgacga
    46981 cgaaatggac cggctctacc attcgatttt tgacgaactc ctggggatca tggctggacc
    47041 cgcgcagggc aaccccgacg cactcctgtc ctccacggca ctcctcgccg tggcccgtca
    47101 cctggagcga atcggcgacc actccgtcaa catcgcggag cgggcctact tcatggtgac
    47161 gggccggcgg ctcaagaaag tgctgcgcga ggcgggcgaa aaaccttcac cgcccttcat
    47221 ccgctagagg ggctcgaatc ccggcctgta aatcagcacg gaacagggag cgtaacgcgc
    47281 cactttcgat gcggtggatc cgaccaccag gctggcgatt cggctgttgc cgcggttccc
    47341 caccacgacc aggtcgaaac gcccgtccct ggcaaaggca atgatctctt ccgccggttc
    47401 cccttcccgt acgaccaggg gatgacggtg gtcctttccg gtgggcatcc tggcctcgga
    47461 ctgcaggacg aggagctcga ggtgttcctg ggccttggcg cggaccgcct cgggcaggct
    47521 gatcatggac gggttcccgt accccctgaa ggcctgggag aattcaatga catggatgaa
    47581 tgagacttcg gcgtcgagac ggtgcgccag atggtgcccg taagtcacca catcgtcccc
    47641 gagccggctg aagtcaagac agaccaggat gcgtttcggc aaggcacttc cccctcactc
    47701 agtagatcgg cactcctccg gagggccgtt gttctcgggc gaatcatccc cggtgcccgg
    47761 cagcaggggc acaaaccggc agtattccga ccagctgtcc tcgaagccgt ccggggtttt
    47821 cagcctcacg agcaggcgtt ccagcccagg ctggatcacc agggggacaa ccagtctccc
    47881 cccctcgccc agctggtccg tccaggcctt ttccacccgg ggggctgccg ccgtcactag
    47941 aacggcgccg taaggagcca gctccggaac ccccaggcgg ccgtccccgt gaaaaacggc
    48001 cacacggtac cctagccttg ccaggcgttc gcgcgccgcg cgcgccaggg cggcgatccg
    48061 ctcgacggaa acgacctcca ttcccatttc agcgaggatc gccgcctggt accccgatcc
    48121 cgttccgatt tccaggaccc gtgtccgatc cgggagcgac agaagttccg tcatgagggc
    48181 gaccatgtac ggctgcgaaa cggtctgtcc ctcaccgatg ggaagcggtc cgtccgcatc
    48241 cgccagggga gcgagatctt ccgggacgaa ctccgtccgg tcgacccgtt ccagggcgga
    48301 aagaatccgc cagtcgcgga tccccctggg acggatctgg gtttcgatca tccgccgggc
    48361 ccgttctctc cagggtttca ttccattccc agcctttccg cgaggacacc ctccagttcg
    48421 gtccagccgg ggattccccg gaaaagcagt ttgccgtcaa ggatccagac gggttctgtg
    48481 ctcttgaccc tgaaccggac cagatcgaag atccagaaag aacaccgcgg gtccaggatg
    48541 tcgatctcaa tccggtcccc gaaggtgtcc cgcaccttca caaggagcct gccgacggac
    48601 tcacggtccg tcctcatcgg gaaagaatcc atttcttctt tcccgtagct gaatccgcct
    48661 cagcaaacgg ccgactgggg gcgctccatg aagatcttca gccggtgttt tctcatggtt
    48721 gtttttcctc ccgcggagaa tccgctccca ttatacgttg cggtaaggaa ctcgaacatg
    48781 gattctgcta agataaagat gagaatcaag ggaaaccggg agcccggttt tgcttctggg
    48841 ccctcggcaa agccgtggaa aggggtgggg agaatgtttg acctgagaga tgcgtttacg
    48901 gtcggaatga cggaagaaac ctcgaaaacg gtggaagcct ccgatacggt ggggaatttt
    48961 tccccgagcc tgaacgagtt cctctcgacg tccgcctgtg tcaacacgat gatccggatg
    49021 gcgatcgaca tgatcgaccg gttccttccc gaagggatga tctccgtcgg caggaaggtc
    49081 gagatctcgc atacggcccc ttcgctcctt ggcgttcacg tccattaccg gatcgtcctg
    49141 acggggatcg acgagaatca cctgcacttt actttcgtgg gctgggatgg gctcggagag
    49201 atctgccacg gacgccacga gcgcgtggtc gtttcccggg aggcgctggt gaggaaggcc
    49261 caggagcggg cgggcttcct gacagaggct tgaggatcga tgctggatct ctcggtggaa
    49321 aaacgggtca actcgaacct ggcggttgcc tcggtggttc tgagcatcgt cgggatcctc
    49381 ggaacggcca tcggcttttt cgggttcatg ggagccttga tggcggcaag cccgctgggt
    49441 gccgtcgggg gaggaggaat cctgctgatc tcccttttgc tgggggtgat cgggacggtg
    49501 ctgcagctgg tggtcgtcta ccagtggggc ggagccctga acacgaacct ggcgaacctc
    49561 cgacttctgc tttcccacct cattgcccat gccggcgatg aaaccgaacg ggtcaattac
    49621 cagaacggcc ttgacctttt agcgggaatg ggggtttcca ctgtcctctt ctgggggtat
    49681 atcctgctgc acctggtcag cgtgttcctc cccggtggtt tgggcaggct ggtggggttc
    49741 gtggcgttcg ttttcatcgc cctctacctg aactccgtct tcaggctcac cgatgcagtc
    49801 caggacttga ggcagaaact ggtggcccat gtgctgggag aaggccccga cctgccgccg
    49861 aagcggatcc gccacaggaa cctgtttctt tccctggttt tcatggtcct gaccctgggg
    49921 atctactggc tattcctgct ctacaacctc accgccgaaa tcaacgccta cctcgacgat
    49981 gaccaggcgg tccgaaggcg gatcctccag gacctggcct gaaagacaac cctccgaaaa
    50041 aaaagggcgg gctccagggc ccgccccctt ttttctcgga accagaggga tcattcgcaa
    50101 ccgtaaagca tccagtcgag cccgagccgt gtcagggttt ccacggaggg aagcccttcc
    50161 tccgggtccc accccatcgc cttgtagaag ctctccaggg cggtcttcag gtcgacgacc
    50221 tgtcccgcgc cggggccgtc ggcccccggt tccttcagga agcgttccgg cagggtgtcg
    50281 tcgctcccat cgaacccgaa aatggcgttc accatgcgct ccaggttgat cacccgctcg
    50341 gcggccagca gcagttcgtc cttttccatc tctctgccga cggctttcga gtgcagtttc
    50401 gcccacgtgg acggcttgat tcccagggtg atgttggtga agagacaggt ccccagcagg
    50461 ttcgggatca tggcgttggc catgaggggc ttgacccagc ccttgtttcc gtcctccgtg
    50521 atggaaatgg ccgcgtcagg ggcgatgccc agctccggct cccggtagcc gaaggcatcg
    50581 atcgtggagg cgtaggggcg caggtgatcc gctccccgag gggaaaccgc gtggacgacg
    50641 gcctcgccct tgctggcccg gactccgtcc gcggccatct ccattccctt gacggccatg
    50701 acggcccctt ccgcccccgg tcccagcctc tcggcggcac ggacgctgcc cagggcaagc
    50761 aggtcaccga cgccttcccg gtttgcaatg agccggacgg cttccacaac agcttctcca
    50821 ttcccgaaag ccaggtcgag accgccgaga cgttctttcg tcaggatgcc cttttccgcc
    50881 cactccatgg ccgtggcgat gacctggccg gtggagatga cgtccaggcc catgtcgttg
    50941 gccaggtggt tggcagcggc tacggcgttc aggtccttca cctcgcactt gctcccgaaa
    51001 gcggccatgg cctcgtattc ggggcctccg ccctccatcc ccgcccattt gccggtcctg
    51061 attttcgtgt ggcgcccgca ggcgatggga cagccgaagc acgcgtaggg cttcacttcc
    51121 agggtttcgt gataggtctt gtaggccatc tcgggaagcg attccggata ggtgtccctt
    51181 tgccagttgc gtgtcggcag gatccccagg ccgcaaatgg catcgtaaaa ggaaggtgtc
    51241 ccgtagggct tcatctcgtc ccggacgaag gcctcgttga agagttcctc ccgggcggcc
    51301 ttggctgctt ccttcagccc ctcgggatcg gcgatgggaa gctcttccgt tccccgaacg
    51361 gcgatggcct tcaggttctt gctgcccatg agggctccgg ctcccccccg gccgaaggcc
    51421 cggtgccggt cggtcatgat ggaggaaaag aggactccct tttccccggc cggcccgatc
    51481 gatgcgatct gccagccgga accttcgagc tggccccgga tccagtcttc cgtcgctccg
    51541 actcccttgc cccaggcctc ttccgcgtcc aggaagtcga cgccgtcgtc gtcgatgacc
    51601 aggacgaccg gtgtttccga tcttcccgtg acgaccagga tgtcatagcc ggctcgtttc
    51661 agggccgggg caaaatgacc gcccgcgttg gaggctccca gagtgcctgt caggggggag
    51721 cggaacacga cggcacaacg cccgctcgcg ggcgccgcgg tacccgtgag ggacccggtg
    51781 gtgaagatca gcggctgctc cggagccaaa agctcggctt ccgggtccac caggtcgagc
    51841 agaaggcgca caccgaggcc ctctcccccg atgtagtccc tgtaccactc tccgggaagg
    51901 agcgactctt ccacggttcg ttcagtgaga tccaccatga gcgcggttcc gaagtagcct
    51961 cccatctttc ctcctccaat cgttttgaga tggtttaaaa actccgtttc caggattcca
    52021 ttatagcgag ttgatgcgct ctctggatga acagcccggc cagggttgtt ctagggggtt
    52081 gcgtttcccg tcatccgctc cagcagggtt cgaaggcgct tgagttcctc cccgtagcgg
    52141 gcccagtccc cggcacgctg ggctgcctcc gcggcttcat aggcctttcg ggcctcctgg
    52201 gcgagtgcct tcaggtcctc tgcccccggt gcggagggcc cggaggaggg gaaaaggctc
    52261 tccggttttg acggacccgc cggcttcccg gcttgaattc ccacgacgcg cctcaaggct
    52321 tcgtcaaagg tttccgccat ggcgacacgg cctcccgtgg aaaggatgac ccgtttcagc
    52381 tcgggaaggt cacccctctc cgccttcagg tagaggggct ggacatagag gagccccttg
    52441 ccgaccggga tgacaagaag gtccccccgg atgacctcgg atcctctctg gctccagagg
    52501 gacagctgcg cggagatctc cgggttctgg tcgatcaggg cctcgatttg ggcgggcccg
    52561 aagatgagct tctgcttggg aaatttgtag accacgagct tcccgtagtt ctccccgtcg
    52621 ctgcggcccg ccatccaggc gatcatgttg ttgcggccca ccggcaggaa ggggatgatc
    52681 agcgcaaact ccggtgcctt ttcgcccatc agcctcagga tggtgtagtt gggagaaacc
    52741 gcctttcggc tgccctcggg gatgagggtc cagacgtctt ccttgttgta gaaggtattc
    52801 gggtccttca tgtggtaggt gcggtagacg gcactctgga tttcaaagag tccttccggg
    52861 tacctcagat gctccctgag ctccggggac agggcggaag cgggcctgaa aaggctggga
    52921 aagacggaac gccacatcgc aaggaggggg tccttctcgt cgatggcgta aaaggtgacc
    52981 gtgccgtcat aggcgtccac cgtggccttc acgctgttgc gcaggtaatt cgctccctgg
    53041 aaattcgaga gcgtcgggtc ggacagggac accggcctgg agtagggata gcggtccgtg
    53101 gctgtgtagg catcctggac ccacaaaagc ttccctccca ggacgaccag gtaggggtcc
    53161 gagtcgtaga gaaggaacgg ggccagctgt ctcagggctc cctggatatt ccggttgtac
    53221 aggacccggc tttcgggacg cagggaaccc gtgaagaaga tttccgtgtc tccgaatcgg
    53281 gttgcgaaca aaagtctccg ccaggcgctt ccgatcccaa ccccgccctt gccctcgtag
    53341 gtgttccgga cgttcgactc tcccatggga tagtcgaact cccgtacgtc ggtcttcacc
    53401 agggcgtagg tgctggtctt ctccccgtag taaatctggg gccggtcgat cgaaaggggg
    53461 acgtccacct tcggagggag gtcccggatg aaaaggatgg gaagccccga ggggcccacc
    53521 tcgttgacgg ggttcatcac cagtccgtag ccgtgggtga attcgaggtg ggtgttgacc
    53581 caggtgcggt tctgcagctg ggctggatcc agctccctgg cggatagcat gacctgccgc
    53641 atctgtccgc cgaaccggta gcggtcgatg tccacgtcgg tgaaatcgta gtaggagcgg
    53701 atttcctgga gctgcttgta ggtccgaaga agcggaccgt agtcccagag gcggatgttc
    53761 cggatggtgt ccgagttggc cgcgactccc tcgggggtta cctcgttctc cggggtcatg
    53821 tcgaggacgg tcacctcgct gagcccgaag gcttccaggg tggctgcgat gtgatagcca
    53881 atgtagggct tttcgcgctc gaactcgttc ggcttgacga cgtacgactg catcagcccg
    53941 gggacaacgc tcctcaggat gaagacggcc gcggccaggc tccccaggat ccaccagagg
    54001 gttttcctgg agcgcccggt acccgtgagc acaaggatcg ccgccgcggc gcagaggaag
    54061 aaaaggacgg acagcgcggg cagaagcgcg tggatgtccg tgtagcccgg gccgaagacg
    54121 atcccggagg gagaatagag caggttccag cggtccagca gaaaaccgct tccccagacc
    54181 aggacgaagg ccgaggccag aatcgacagg tgccttcggg tctggtcccg gaggctgaag
    54241 tcgctggcga agaagcgccc cagctttccg gggagcgcca ggacggccgt tccccccagg
    54301 gccaggaaga gcccgttcag gagccaactt cggatcaggg ccaaaaacgg aagcgtgaac
    54361 acgtaaaacc cgatgtcctt cccgaaaatg gggtctttct gcccgaaggg tcccctgtac
    54421 aagaacccca ggatggtttc ccaggccggg cttcccccga ggctgttgcc cgcggacaga
    54481 acggctgccg ctcccaggat gagccagggc agaaagcgcg ggttccaccg gaccgaccag
    54541 gcttcggaca gttcatcttt cgaacgtttc catgcagaga tccagttgaa ggccaggaag
    54601 gcgaaaaaca ggagcgacga cccgaggaaa agggtgatcc tcgtgaggat ccgcgtgcgg
    54661 aagacggcgg aaaaccccag ggaatcgaac cagagccagt ccgtgtagaa acgggcaaag
    54721 gcgggccagg caatgaaaag cacgaggagg agggggagga ggatccagaa gcgcctcccc
    54781 ctcccgaaga ttcgtccgga acgctcccgg ggagttttct cgggggcttc cccttgaaaa
    54841 ggcggccagg tctttctccc ctcgaaggtc cagccgtcct cgttcatgct ccggtccttc
    54901 ctgcccggct gaggaggaat tccagcattg tccgtgttcc gaatcccgta aagcccttcg
    54961 ggacgtagcc gtactcctcc ttgctgtgat cggttccggc catgtcgata tgggcccagg
    55021 cgatgcccct cttgacgaat tcctccagga acatggctgc gaagatggcc cctccgtaac
    55081 ggctgccggc gttcaccagg tcggcgacgt ccgatttcag ttttttccga agccgttcgt
    55141 catccatggg gaacggccag agccgctcgc cggaaaggcg ggaagcctcc atgagctgac
    55201 cgacaagcgt ttcgtcgggg gaaaagaggc ccgcggtgta gtctcccagg gcgatgtgac
    55261 aggatcccgt gagcgtggcg atatccagca cgacggacgg ttcgaggtcg ctggcgtagc
    55321 agagaacgtc cgcaagcgtc aggcgtccct cggcatccgt gttgtcgatc tcgatcgttt
    55381 tgccgttctt tgcccgaagg atgtcgtcgg gacggtacgc gtctcctccc ggcatgttct
    55441 cggccgcacc gatcagcccg tggacctcca ggggcagacc cagggcactg acggcgctca
    55501 tggcggccag caccgcgcag gccccggact tgtcgccttt catggttctc atgaaatcgc
    55561 cggtcttgat gttcagcccg ccgctgtcga acgtcagccc tttcccgacc agggcgatcc
    55621 gctcccgtgc ggcttccccc ggcttccagg ccaggtggat gaggcggggc ttgttcttcg
    55681 agccttttcc gacgctccac agggcttcca ttcccatctg gcggatcgcc tcgtcatcgt
    55741 agatggtgca ctcgagcccc gattcctcgg agatccttcg ggcggtttcc gccagcgatt
    55801 ccggggtgat gacgttgccc ggttcgttgg cgaggtcacg ggcgtagttc tgtgcctctc
    55861 ccaggattgc cccggtttga agtcccgctt catccccgtc agccaaaagc agggtctcca
    55921 cttcgaaacg gggcggctcg tcttcgcttc ggccgcggta tttctcgaag ctgtagcctg
    55981 ccaggatcgc cccttccgcg gcggccttgg acagggccca cccggggggg tccggcagaa
    56041 cgaacaggac acgggctccc cggtcccgaa gtgcggccct cacgatctcg ccggcagcct
    56101 gccggaccgt ttcctgcccc agcggaccct ttttgcccag tcccaccatc gccagcgtga
    56161 gttcaccggg gaggggaaga agcagcactt cccccgcctt ccccttgtag gcccgctttt
    56221 cggccaaggc tttcagccgc tccgtccagc cggtttccag ccgttccaga gtgctccagt
    56281 catcctcgaa aacccccgcg gccaggaaat cgcacggcca gttcccccgg gtgatctcga
    56341 tcgcccgaac ttccacatcg atcgcctcct ggaaaatctt ttttcgttat gacgctattc
    56401 tagcagtttc tttccttatg gagctttaac atgctatcat tcccctgaca ggaggtgctg
    56461 gccgtgtttg gaagactgct ttttgtggac ctcgcgtccg gaaagattca ggaccgcttc
    56521 cttaccgctt cggccgtgag ccgtttcctg ggaggcaagg ggctgggaat cgcccttctt
    56581 gaggaactgg cttcccctgg aggcgatccg ctggattttg cgaacccgct ctgctttttg
    56641 accggacctc ttaccgggac gcccttcccg acctccggcc gtttcaccgt cgctacccgt
    56701 tctcccctca cggggctttg gctagacagc catgccggcg ggcactttgc ccctttcctc
    56761 aggagggccg gctacgacgg gatcgtcttc acgggccggg cggatgttcc cgtttacctg
    56821 tggctttgcg acggaaaggc agaattgcgg gaagcggctg gcttctgggg gcagggagtg
    56881 gaagaaacgg tccggagggt ccgggaggaa accgaccctc aggcccacgt ggcctccatc
    56941 ggcccagcgg gggaaaacag ggtccgcttt gcctccatcg ccgtcgaccg cgactctgat
    57001 ccctggcggg cggggatggc cggccgggga ggcgccggtg ccgtcatggg atccaagaac
    57061 ctgaaggccg tggcggtgaa gggctcaaag ccggtccccc tggcggacga agccggactg
    57121 aaggcactcg tgaaagaact cttggagcgg gtgatgaaaa acccgggggt ccatagcctc
    57181 cgggttctcg ggaccgctgc cctcgtggaa ccgatgaacc gcacgggaat cctgccctgc
    57241 ttcaatttcc gcaagggctt cctggaaaac gcccatgctc tcaacggggc gaacctccgc
    57301 taccatgcga aacgggacgc gggctgcttc aactgcccga tcgtctgcgg gcgggtgatg
    57361 ccctcggacg ggcgcgacgt gaaagtcgag tacgaggcgc tggccctgct gggggcctcc
    57421 tgcggtgtga ccgacgtggc ggacgtggct cgggcaatcg ctgtctgcaa cgacatgggg
    57481 atggacgtga tctcggcggg gggcgtggtg gcgtttgcca tggatgccgc atcccggggc
    57541 ctgctcgaag aagtccccga gttcggggac gccgccgggc aggtgaggct tctggaagcc
    57601 atcgcccggc gtgaaggcat cggggacctc ctggcggagg ggtcaaggaa ggcgtccgaa
    57661 gcaatcggcg tggaggcctc ggaactggcc gtccacgtga agggcctgga gctgccgggg
    57721 tacgacccgc gctcgagctg ggggatggcc ctggcttacg cgacagcgga ccggggggga
    57781 tgccaccagc gcgcctggac agtcatgcct gagatcgacg gggcggtcga gcggttctcg
    57841 gccgaaggga ccgcccggat cgtcaagaac ctgcaggatg accgcgccgc ggcgtattct
    57901 ctcgtggtct gcgatttcct tcccatggaa acggaggatg ccctgaaagg gctcaaggcc
    57961 gccagcggga tcgaactgga cgaatcggga taccttgaga tcggggaaag agtatggaac
    58021 ctggccagga tgttcaacct caggcatgcc gggccggaag gcatccagga cagccttccg
    58081 cagaggctcc tccgggagcc cctgcccatg cccccccagg ggaaaacggc cgtttcgctg
    58141 acggtttggg acctgaagct gatgctggag gattattact ccctgcgggg ctgggatgcc
    58201 cagggacacc ccagggaatc gaccctgaag cgcctgggcc tgtcggaggg cttcagggag
    58261 tccatcctgg agaaagggga gaagtagcca tgtggaacat ttgcctggtg ggattcggca
    58321 atgtcgccca ggggcttgcc cgcatccttg ccgacaagcg ggagcggctt gccgcgaaat
    58381 acggtttcgg attccggatc acgtcggtcg tcacggcccg gcggggctgc ctggcgaacg
    58441 atcaggggct cgatccagcg atgctgctgg aagccatgga aagggacgga gggctctggg
    58501 gatgcgaggg agcccgggtg atcgacccgc tggaagccat caagagcccc cacacggaca
    58561 tcctggttga aaccaccccc accagcctcg tgcgcggcga accgggtctc acgcatatcc
    58621 gcaccgcgct caaggcgaag aagcatgtcg tgacctccaa caaggggccc ctggtccggg
    58681 caatgtcgga gctgacggcc ctggccgagg gatgcggggt cgcgttccgc tacgagggag
    58741 tgatcatgag cgggacaccg gccctgaacc tggtcaggga aaccctggcc gggtgcgaca
    58801 tccagcacgt ccaggggatt gtcaacggca ccaccaactt catcctgacc gagatggaaa
    58861 agggaaaaac ctacgaggaa gcgctagcag aggcccagcg gatcgggatt gccgaagcgg
    58921 acccgactgc cgacgtggag ggatgggatg cggcggtgaa agcctgcatc atggcggtcc
    58981 tgttcatgga cgcaacgctg gagctggagg acgtcgaacg gacgggaatc acggaagtga
    59041 gccccgaaat gatccagacg gccctttcca ggggcaaacg catcaagctg gtggcggagg
    59101 tctaccggga aaagggccga gtcaaggccc tggtcgcccc ggtggcggtc ccgctcacgc
    59161 gccccctcgc cggcatcatg ggggcgatga acgccatcac gtacacgacg gaccatctcg
    59221 gagacatcac gattgtggga ccgggggccg gtcgcatcga aaccgggcag gcgctcctgt
    59281 ccgacctgct ggcgattcac cgtgtctacg ggagggaaga gcacccttcc aggagccctt
    59341 tctagctttt ttcgccccgg gcggccagaa cgatcccggc caggatgagc gcgccgcctg
    59401 cggttttcca gagagtcagg gtttcaccga agaagatcca ggccaggagg gtgcttccga
    59461 tcggttctcc cagaaggcag acggcgaccg tgctcgctga gaaccagcga agggcccagt
    59521 tgtaggaaga atggccgatg acctggggaa tcagggccat gccggcgaaa gcccaccagg
    59581 ttggcgccga aaatccggtg aagggagttc tcgaggcgag gaccaggatc cagaggagaa
    59641 ctccggctgt cccgtaacag gtcgtcacgt aacccaaaag agaaagcctg gggcgcaccc
    59701 tgcgccccag catgatgtac agggccccgg aaaaccctcc cgcaagtgcg agggcatctc
    59761 caagaagggc gcggccgccg agggcgaaat cgcccgcccc gatcaggatg ccgcccgtga
    59821 agctcagggc aattcccgca agggtggcct ttccgggacg ttccccggtc accagcgggg
    59881 tcaggatccc tacccagagg ggaatcgttt ccacgagaac gacactgctc gcaacggagg
    59941 tataccccag ggacgcgatc caggtggcga aatgcactgc caggaaaaaa ccggccccca
    60001 ggacggaaag ccaatcccca tgatggaggg cggaaagctc tttcgggcat tttcgggaac
    60061 agaagggtgc cagaaccagc gtcgccaggc cgacccgata ggcggcaatg gtgagagcgg
    60121 gagcttccgc gacccgggca aagatggctc cggtcgaaat ggccgcgatg ccaacggtaa
    60181 gcgcaatgac gggattgaga acgggttttc cggattctgc catgtcttga gtttagcatg
    60241 aaattccctt gcgcctcccc ggggtcaatg ccgataatgg ccctgcagag gagggaaaac
    60301 ccatggaacg gaaaaggagg agggccctgg ctgccggaat cgccgcggcg gtttcctggg
    60361 gggcatcgtt cgtggccatc aaggtggccc tccggaccct gaccccgctt cctctcgtct
    60421 ggctgcgctt tgggctcgga acggcggtgc tctggggctt tgcggccctc cgcagggaaa
    60481 aggaccccct atcggctcgg gaatgggggc ttgttgtcct cctcgggttt ctcggtgtcc
    60541 cgtttcacca tctcgttcag agcacgggac tgaaaaccgc ccaggccgcg acgtcggcct
    60601 ggatcatttc cagcatgccc gttttcatcg cgatcctggg aagcctgttc ctgggagaga
    60661 aaatgtcctt ccgccgctgg ttcggcctgg cctgcggcgc ctgcggcgtc cttctcgtcg
    60721 tcacaagggg ggatcctggc cgcctgcgct tttcgaaccc cggttctttc ggagacctcc
    60781 tcgttctcct gagcgccctc aactgggctg tcttcagcgt catgtcccgc aattttctca
    60841 agcggcacga gccggccccc atgatgctca aggtcatgct cgccggatgg ctcatgacga
    60901 ccgccctgtt cgcggtgaag ggagacctgg ggccgatcct ccggatgaac ttccaggcgt
    60961 gggtgtccgt ccttttcctg gggattttct gctcggggct ggcctacgtt ttctggtacg
    61021 acgcgctgaa aatccttccc gcctcgcagg cgggggtcct gatgtacctc aatccggtat
    61081 ctgccaccct tcttgcggtt tcgggtctgg gcgaacccct ttccgcggcc atgatcctgg
    61141 gggcggttct gatcactgcg ggcgtctggc aggtcagccg ctgaccggga ccgttttccg
    61201 ggcgggcgtc tcgaccaggt agggcctcgc actcggaggg agcacctcca ggatgtcggg
    61261 aaaggtgtag gtgtcccgac gggtgcggat gaaccgtacg cgggccggcg gggaatccag
    61321 tcgatacgtg gtgcccgcct ggacctgatc ggtgaggatc ttttcaaggg tttccctgcg
    61381 ggcggcccgg gcctctctcc gggattgcag cgaaagggct gctgacagga gcgaacgggc
    61441 ccctcccttc tcggcctcca gcgccagggc ttctccctcg gcctggagaa cctcctcttc
    61501 gccgggctcc cattcgacga ggatcttttt cgccctcctg gacacgacga gtcctagctt
    61561 cctggcattt tcctccaggt cccttcgtcg cttcaggtac cttctgagcc gcttcgtgtt
    61621 gagcccgcga acggtctccg cctgaagttc ggactccttg tcgagctggc cgaaatccag
    61681 tccggagtgg atgagcccgg ccaggctctc ctccagcttt tgccggagga aagcatcccg
    61741 cgcctcatcg agggcgatcc cggcaagttc ggccacgagg ctgtcgagct cctcggccgt
    61801 gagagaaggg gaaacggcgc gagagcggat ctccggcccc ggtccttcga tctcgataaa
    61861 ctccacctcc gggtgctccc actggcgtcc ccggaggatg ggctccgcga aggaatcccc
    61921 gaaaaccagc ctcccgatgt tgatggcggc gttgatatcc cggtggatcg cagcgcccgt
    61981 ggtgctatgg tactcctttc gtgtcaggcg ccccgtcgcc cggtctttcc ttcggcttcc
    62041 tgcccccgca acgggttcca ggccgagggc gtcgatgcgg ctggtattcg tttcatcgac
    62101 gaggtggacg tcgatccttg ccagggcgca ccggaaacgg agggtgctca gcaagtgaag
    62161 gctcaggttg aggagagggt tgtcccgaag ggcccggggg cggatggaga acgggttctt
    62221 gatcgagcgg gtgccgatgg cgatcgaacc cacctgccac tccaggcatt ttcgcaggac
    62281 caggtcgcag gtcgcgtcca cgcaggaggc gatgcggccc atttccgtgt ggaatttctc
    62341 gggttccgac tgcatggatc gccgtgcccg gtcgatcgct gcccggtcga gcgggttctt
    62401 cacgagcact gcccgggaca ggggtgcatt ggaaatcatt gcgaacaggt aagtgtcgcc
    62461 gatgtcgatg gccagggccc tgtctttcgg gttcatttcc acggtcatca cctccccgta
    62521 gaacacaaaa ggcggcaggg agaaaaaacc cctgccgcct cgaaagcgga ctcttttcgc
    62581 cttttcgggt tttccggagc gcttctgccg ttcattcccg gcatcctgtc atccccccga
    62641 gccccttttc acttgtaatt ttagacgttc tcccgggtaa aggcaagact g
//
LOCUS       DAOOVN010000003        58404 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_311_length_58404_cov_16.099230, whole genome shotgun sequence.
ACCESSION   DAOOVN010000003 DAOOVN010000000
VERSION     DAOOVN010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 58404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 58404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58404
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_311_length_58404_cov_16.099230"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PLY83_00640"
     CDS             complement(<1..294)
                     /locus_tag="PLY83_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV28576.1"
                     /translation="MTEREAALLVLNACEGFHARHLEGWSRRGLLPEEVLEGGRACWK
                     DLGFSEGCQEKLEGLREKQWGSKEAERSSRLGIRLVFWGTPDYPEAFLDLSRPP"
     gene            complement(281..1792)
                     /locus_tag="PLY83_00645"
     CDS             complement(281..1792)
                     /locus_tag="PLY83_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOV28577.1"
                     /translation="MGPILGVTLKGIEAIPIDIEVEITGGLFVISVVGLPDAAVREAR
                     ERVRAALRSNGIQLRGRIALNLAPADLPKVGSLLDLPMAVGLAMESGAVRVGETALFL
                     GELAMDGRLRTTKGAVPAALLARKMEIPLFLPPGNAREVALVKGVKAFAPPDLKSLLA
                     HLRGESRIPPVTESSAETEDKVIEPDLADIKGQAAAKRALEIAAAGHHNLLLMGSPGS
                     GKTLLARALRGILPPLSDEERLETLLVKSTLGIPAKPDRTRPYRVVHHTASAIALCGG
                     GTTLRPGEISLAHRGVLFLDEFPEFHRDLLEALRQPLEDGIITVSRASGSVTYPARVL
                     LVCACNPCPCGYWGDPVEPCRCSAHTLEKYRNRLSGPILDRIDLHVSVPRLTPEELVC
                     LEGRQGPSSREIREKVTAARKIQQERWKAQGFGCNAEIPERLVRSGLCLSRKVRPFLM
                     EAVRGLRLSGRGISRVLKVARTIADLRGDLEVSVEDVCEALAYRGGGNPIDGA"
     gene            complement(1792..2160)
                     /locus_tag="PLY83_00650"
     CDS             complement(1792..2160)
                     /locus_tag="PLY83_00650"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOV28578.1"
                     /translation="MDHLETGRLGEALAERFFRAKGWTILGRNIRVRGGEIDLVAMDG
                     PELVLIEVRTRSIGKILPAEETVGPRKLKALVRSGRIYVDRCRWQGPWRIDLLGINLD
                     EEGHPRFSHLPDITVGRVQV"
     gene            complement(2216..2788)
                     /locus_tag="PLY83_00655"
     CDS             complement(2216..2788)
                     /locus_tag="PLY83_00655"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOV28579.1"
                     /translation="MTKIAGVDEAGRGPLAGPVVAAAVILTPAQRKVLVGLGLDDSKR
                     LPPSRREALFSTMLELGVRWKAQAASAGRIDSMNILNATLWAMRACVLGLDPEAELVV
                     VDGNRPIAGLPVRQKAIPRADQLVPAVSAASVIAKVLRDRVMVQLACLFPQYGFEIHK
                     GYPTKAHREALKEYGPCEIHRISFCRKILS"
     gene            complement(2785..3624)
                     /locus_tag="PLY83_00660"
     CDS             complement(2785..3624)
                     /locus_tag="PLY83_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOV28580.1"
                     /translation="MGRTVWFPGHMARGQKRLFEVVANLDAVVEVRDARAPALTASPL
                     SERLARMKPVVLVLSRRDLAEEAKTVRWLENFRKSGRTALAFNLRQDRLDALKAELHR
                     YKPSHRDLRIGIVGIPNVGKSLFLNGLVGRNASRVGALPGITRGVEWFRGQGVLVVDS
                     PGILQAKTGLKTGRALSWLGCNRADVIGSFESCALALIAFLGDQGLWPVVEKAWGVTL
                     DPAGPEATLDALGRRLGCLVSGGSIDAGAAARKFLESFASGRLGRVTLELPESPLTEE
                     NLP"
     gene            complement(3629..4141)
                     /gene="lepB"
                     /locus_tag="PLY83_00665"
     CDS             complement(3629..4141)
                     /gene="lepB"
                     /locus_tag="PLY83_00665"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOV28581.1"
                     /translation="MPAKPWWRETIETILWALVLALILRAFVVQAFWIPSGSMIPTLD
                     PGDRVLVAKFWHALSEPKRGQIIVFKYPVDPSRDFVKRIIALPGETVRMENGTVFVNG
                     KALSEPYVVNHDDFNMPEMKVPEKHYFVMGDNRPNSQDSRFWGFVPRSFIKGPAFLRY
                     WPLSRIGLLN"
     gene            complement(4196..4726)
                     /locus_tag="PLY83_00670"
     CDS             complement(4196..4726)
                     /locus_tag="PLY83_00670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOV28582.1"
                     /translation="MSRGTRRNAKPRQRREAERPLPSASCGFSLVETLVVCAIMGILA
                     GAVGVSITALVQWERSEGAVVRQAEAVQRWLDVALEKGLLRQLSFTLKLPASPSGKLV
                     LVWQGQDFTQSEIYDSMGKCDFAVAGGKAVTAVYNPCYHSVSPGFTLKILPPGKRAPV
                     RSIIVSPYARVRISLP"
     gene            complement(4710..5270)
                     /locus_tag="PLY83_00675"
     CDS             complement(4710..5270)
                     /locus_tag="PLY83_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOV28583.1"
                     /translation="MQYVIVGLGGQGILFTSRVLGHIAVSRGERVMGSEVHGMAQRGG
                     SVISHFKVGPYESPLVLAGSADLLIAFDQGEGIRNLHFLREGAQAILNLHDPAALENP
                     HLKKYAADRSLSIAGIPGYQILREKMGGRFLFMNVLMLGAMCGINAGNVSIDEIRETV
                     AKLAPEKHKADNLKAVDLGYGYFKGN"
     gene            complement(5274..7130)
                     /gene="iorA"
                     /locus_tag="PLY83_00680"
     CDS             complement(5274..7130)
                     /gene="iorA"
                     /locus_tag="PLY83_00680"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HOV28584.1"
                     /translation="MGNKVILLGNEAIARGIVEAGCEVASAYPGTPSSEILPAVARYA
                     DELGTRTAVEWAVNEKVAFEVAATASFTGKRTCAIMKQVGLNVAADPLMSVAHFDLAG
                     GFLIVSADDPGPHSSQTEQDSRYFAMFAKIPCFDPASAQEAKNMVFDAFDLSEKYHMP
                     VMLRPAVRVDHCRQDVELGAVKDLGRKADFKKDIRRWVCLPAHVRITHPQLNAKLDSV
                     REDFETLYGRYNYEVPARGRASLGIIAAGVSFSIVMDLLKAWKREDISVLKIGTPHPL
                     PVRMVEDFIARHENVLVLEETYPVIEMQLPDRTHLKGRWNGFVPRAGELLPEVIRDLI
                     LKALGEPLPVEENSELKAAIEELKVTPKPPMLCPGCPHRASFFAMRKALPNGIYPSDI
                     GCYTLGVNQKDVDTTIDMGASLTSASGFYLAYKTDGKDQPVVATIGDSTFFHMGILGL
                     VNAVYNRHAFVLAILDNRITAMTGGQSNPSIGDKLRKGDEGRQVDIEGVVRGCGVSFV
                     KVVEPYDTTESAEVFKAAWEHARTHREPAVVILRHPCMLLRQKQAIVKVRVNPDKCIG
                     CKYCTNFFNCPGLPFDEAAKKAKIDERFCVQCGVCIQVCPHGAIEAVEGEEE"
     gene            complement(7123..7575)
                     /locus_tag="PLY83_00685"
     CDS             complement(7123..7575)
                     /locus_tag="PLY83_00685"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOV28585.1"
                     /translation="MEERSSGPAARIRVRYAETDAMGVAYHANYLVWFESGRTEFCRL
                     KGLPYREWEQAGVFLPAVEARCRYKHPARYDDLLDVLVDLSDLSPYTVTFSYRILRSE
                     DGLLLAEGQTKHGFCDRNGKLLKAPEPFFGWLQKIIGQAREEGVVSVG"
     gene            complement(7676..8110)
                     /locus_tag="PLY83_00690"
     CDS             complement(7676..8110)
                     /locus_tag="PLY83_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HOV28586.1"
                     /translation="MSKGRVVAVCRSDRKGIPKEDIRTGRLIEELGLEGDAHAGFAHR
                     QVSLIAMEDICKAQEKLPGLRPGSFAENVTVEGIDLGESRVGRRIRVGETLLEITQIG
                     KECHTKCEIYRTTGDCIMPKKGIFCRVIQGGIVSAGDPVEWE"
     gene            complement(8103..8549)
                     /gene="nifU"
                     /locus_tag="PLY83_00695"
     CDS             complement(8103..8549)
                     /gene="nifU"
                     /locus_tag="PLY83_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HOV28587.1"
                     /translation="MYNEKVIQAFMNPKNVGAMENPDGVGEVGNPRCGDVMRIYLRVR
                     DDRIEDIRFETFGCAAAIATSSTATEMVKGKTIEEALKLTNREVAEALGGLPPEKIHC
                     SLLAEEGIRAAIEDYRRRRQGLPPLPPRHDAVCHHDIPGMEEGHGE"
     gene            complement(8572..9744)
                     /gene="nifS"
                     /locus_tag="PLY83_00700"
     CDS             complement(8572..9744)
                     /gene="nifS"
                     /locus_tag="PLY83_00700"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HOV28588.1"
                     /translation="MDLRRIYMDHSATTCVDPSVLEKMLPFFSEKYGNPNSVHAWGRE
                     VRTAIDEARASVARLLNAEPREILFTGGGSEADNLAIKGVAESFSGKGRHVITSAIEH
                     HAVLDTVKWLGKEGFDVTILPVDSEGRVRVEDFRAAIRPDTILATVMYANNEVGTVQP
                     IRELGEICREKGILFHTDAVQAAGHLPLNVRDLPVDLMTMAAHKMYGPKGVGALYVRK
                     GVRIAPLVHGGGQEFGLRSGTENTAGIVGFGAAAELAARRIANGEIDKERRLRDRLID
                     GVLSRIEDVLLTGHRTERLPFHASFCIRYIEGEAMLLRLDFAGIGASSGSACTSGSLE
                     PSYVLLAMGLDHATAHGSLRLTLGKDTTEEDIDTILNTLPPIVENLRAMSPFKKNA"
     gene            complement(9854..10270)
                     /locus_tag="PLY83_00705"
     CDS             complement(9854..10270)
                     /locus_tag="PLY83_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOV28589.1"
                     /translation="MTKEKEEVIREFVCKPSAIAIVGASSNRERPVFAVMSYLLERGF
                     RLFPVNPAYAGQKVLDLDCAASLLELSQNVDVVALFVGAKNQEPILRDLREMPYRPVV
                     WMQPGAENAEAEKALQVEGFPVVSGACLMATHRTHC"
     gene            complement(10328..10714)
                     /locus_tag="PLY83_00710"
     CDS             complement(10328..10714)
                     /locus_tag="PLY83_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV28590.1"
                     /translation="MSVRFKKWIFWLVSLVCLLVVSPPCLAGCAVDPGVSLAITKLGW
                     SLDPKTGSLQVEAEILNVSEWDVVDPGIAVSLLDSKGHEFGSWVVRSSLKRIRPNEQG
                     RLSLRVKIPSIPGTIRVLPYQGNTGT"
     gene            10809..10896
                     /locus_tag="PLY83_00715"
     tRNA            10809..10896
                     /locus_tag="PLY83_00715"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10843..10845,aa:Ser,seq:tga)
     gene            11020..14181
                     /locus_tag="PLY83_00720"
     CDS             11020..14181
                     /locus_tag="PLY83_00720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOV28591.1"
                     /translation="MGDFLFFDLTNEPFFIAPSPRGAGVSGAHEPETARSFDELRQLL
                     PKGAFQALLFRIPPDASLKETVSRLEEICTDVPILFLCDETQAEEASKRIGRGLHGCI
                     DRKGLTWENLQHSIQSASALRNVLGANSRLKKELAETRLDVELHRKAYRRISEDLRSA
                     VRTMSSLGELHRLFFHAQDEKSTLRNFCRAIVKGHGYQMAWVGRILESEEGIRIEPIA
                     HAGDEQGFLATPFPGNIPLRGILEGHPWAVQNIREEAKRFEWAGEALQRRYRSVAVIP
                     FFRPDRNSRMAFCVFDGREGRFSADRLAMLADLAKLLLRGLSLLEEKIQKQADDETLK
                     LMKQAVETSVSGFVIFGLEEGIQYVNEAALRLAGYSSQEEVIGRSYRDFVLVNEKTPQ
                     ILETVFRGGNWTGEFDLIRRDGSLREVFSSVSAVQREEEETRFVLVSFTDISEKRKAE
                     QALLESRRLYEAIIEDQTDMILRFLPETLEITYANLPYARYYGKSPGELIGQSFRCRY
                     TPEEEGRIREHLRKLTPQEPVRQIEERVIHPDGEIRWQQWIDRGIFDSEGNLVEVQGV
                     GRDITELKIAQQNLLREKTRLMTLFDNSPDGIVFCTAEQVIESANKAFLELFGFSAPE
                     VLGRKIDDLLHEPPDERAAALELNLASQDGRPRSLEGTRIGKNGVPVFVSVTVIPRKE
                     PGGEISGTFAIYRDLTERRQKEQALKISSTIIEGSPVVVFRWKAEKGWPVEYVSDNIR
                     QFGYTPEELTAPGFHYAKIIHPEDLPRVESETEALEAKGSSSFCHQYRIVMKNGEHRW
                     VVERNRPERDSSGGLLYHFGVVIDDTERRNAEMEAKKSHEALQKSLRKLERSFLQTIE
                     VLSSTTEARDPYTAGHQRKVALLSEALARRLGLPEKTCEGIYLAAMVHDLGKISIPAE
                     ILSKPSRLNDFEMALIRTHPESAYEILKNVEARWDLAEIVRQHHERWDGSGYPRGLRG
                     EEILLEARILAVADVVEAIASDRPYRPGLGLKAAMAMLEESKGREFDPQVAQACLDLL
                     REGFDFQGEIAFAPDPWGKAGTP"
     gene            14319..14816
                     /locus_tag="PLY83_00725"
     CDS             14319..14816
                     /locus_tag="PLY83_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOV28592.1"
                     /translation="MVKLETSKGDILIALDEEKAPETTANFIQYVRDGHYDGTIFHRV
                     IDGFMIQGGGFDREMNQKQTRPPIRNEAENGLKNEAYTIAMARTSDPHSATAQFFINV
                     KDNDFLNFSSRTPQGWGYAVFGKVIAGRDVVDAIKSVRTGNRGFHRDVPLEPVLILKA
                     SIVEP"
     gene            complement(14870..16816)
                     /gene="glgB"
                     /locus_tag="PLY83_00730"
     CDS             complement(14870..16816)
                     /gene="glgB"
                     /locus_tag="PLY83_00730"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HOV28593.1"
                     /translation="MTKETAGEALHGSVFSAQDLYLFREGTHRRLYRMMGAHLAEEDG
                     KAGVHFALWAPNAEGVSVIGDFNGWDCRKNPLQMIWRDSGIWEGFVPGARQGDRYKYR
                     IWGKGGIQIPDKLDPFGFYSEIPPGTASVVWDLEKNWQDGGWMEGRASRGRLTEPMLI
                     YEVHLGSWRRSPGDGSPLPYREIAPLLAEYVREMGFSHVELLPVMEHPFYGSWGYQTL
                     GYFAPTSRYGTPQDFMYLVETLHRAGIGVILDWVPSHFPTDPHGLAQFDGTHLYEHLD
                     PRQGFHPDWKSAIFNYGRNEVRSFLLSSAAFWLDRYHADGLRVDAVASMLYLDYSRKA
                     GEWIPNRFGGRENLEAVEFLRQLNAACYGDFEGIQMVAEESTAWPMVTRPVHLGGLGF
                     GLKWNMGWMHDILDYFGKDPIYRKHLHDRLTFSIWYAFSENFLLPLSHDEVVYGKGSL
                     YGKMPGDPWKKFANLRLLLGFQGAHPGKKLLFMGGEFGQEREWDHERELDWSLLENPG
                     NAGTARWVKDLNRFLKETPALHEGDFDPSGFEWVDFRDVDNSIVAFLRKRPGERPDPV
                     LVVCNFTPVPRHDYRIGVPVSGFWRERLNSDAKEYGGSGQGNMGGVKSEPVPFQGFAQ
                     SVALTLPPLGILFLVPEEEHPERN"
     gene            17797..19734
                     /locus_tag="PLY83_00735"
     CDS             17797..19734
                     /locus_tag="PLY83_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOV28594.1"
                     /translation="MNGWNGKSLWIDLTLGKLETREIPFPLLESFVGGRGLNDRVLFD
                     TIKEGANPLGPENVLALAVGPLSGTILSSTSRVQVSTLSPLSGILGDGNAGGTFGLCM
                     KRSGFDQFVFTGSSPKPVYLLVDGLHAVLVEASDLIQTSTWEKTDLLKQRHGPGTSVA
                     AIGQAGENLVRFASVIIDRHHSAARGSGAVMGSKNLLAIAVKGHRETPLADPATFRIL
                     AETDRDFLRNDPFQRDPIARFGSHIGMIRWFPGSRNNACYLGPDEVPRSLLPETLKTW
                     EVGRTSCAGCVVPCKDVFRIPSGPYAGEIGKAMEHECLHCLGTNCGIEDPVALMTMEN
                     LADKYGMCVIGLGNTLALAKDLFAHGLIGLEETGGLDLSWENAGAQIELIHQTALRQG
                     FGNLIAEGMLNLARIIGGRAMDFCYHVKGLGRGHHPAGIFSLAHATSTRGADHLRGRS
                     WAFGENDPDIWPMLIREGFIPVDDPVRTLFISERAVTLTDMIGRCKGAVNNWASAVPL
                     VASHPLLRGEALLLSSATGVPFTEAMLEKASDRVYLLEMAFNARQKVRRRDDRFPVRW
                     DRIGSDWAEQERKKHEAMLDRYYALRGCDPETGIPGQESLTRLGLDFAAKALESIDPG
                     AQWVGPPLWSSERYPRGTRRC"
     gene            complement(19769..20947)
                     /locus_tag="PLY83_00740"
     CDS             complement(19769..20947)
                     /locus_tag="PLY83_00740"
                     /inference="COORDINATES: protein motif:HMM:NF019585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HOV28595.1"
                     /translation="MDTGYETRILRIVSRKGKEARLILEENRFHVSGGGQPGDKGRLA
                     ASGFSAEVTDCFRDGEEEVLAVRILQGEPAEGLAVLAEVDLDRQALLSRMHSGEHILS
                     RVLESRHEGLTVYKVAVGEEETTVFLKWDRQIDWPLLFEAEDEANEIIARDLPVTIRL
                     FPPEEAQEIEGLKANWDRLGKEPIRVVTIPGFDCIACSGTHVSSTAQVGGVLVTSYRG
                     TAPDWEVKFTVHRDRVLKEYSRVVRVLLRKVSCPLEKLEKVYDRLQEERQALLRSLDK
                     AKGMLSLPWERETLGGSPLFVSVLPGILPEMAVPGLKSLVESDPDSVGLVLVPSGEGS
                     EGSFILARGARAKIDLRKVIREQPKLMARGGGSESWVTGSTGAAVAKLWIETLQNEIA
                     "
     gene            21046..22149
                     /locus_tag="PLY83_00745"
     CDS             21046..22149
                     /locus_tag="PLY83_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOV28596.1"
                     /translation="MTSDVFFTGTRTTSRRNLLDRLEELVLAAGLDDLPLKGRMTAVK
                     IHFGEPGNLATIRPNYVARLVRMIRERGGKTFLTDANTLYKGQRANAVDHLQAAMENG
                     YNPLSAGCPVVIADGLWGFDYREIEVNLKHFARAKIASAIAEADVLISMNHFKGHEQA
                     GFGGALKNLGMGSGSREGKMAMHCDSPPRIQSARCVGCGECVRNCAHGAVRLTPERKA
                     LIDQTLCVGCGQCVVVCPAGAAQISWGSAKTLGERIAEYAFAALKGKPAFHVNFLMDV
                     SPNCDCWNHNDTPIVPDIGIAASKDPVALDQASVDLVNQAPVLPGSALQKTGEHLEDK
                     FQALHPDADWERCLVHAEAIGLGTRSYRRVDLP"
     gene            complement(22125..23285)
                     /locus_tag="PLY83_00750"
     CDS             complement(22125..23285)
                     /locus_tag="PLY83_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HOV28597.1"
                     /translation="MKIERFGVEEWMNAYEEKARFNIAETCVESLTVEELLGLSGGKE
                     NFLEELLSRKLTYGEIPGAFDLRAHISRLYETLDEKNILLAHGGIGANFLALFTLVEP
                     GDRVVAVKPTYQQLYSVPEALGADVRILRLLPENGFLPDLDDLEKLAGDRCKLIVLNN
                     PNNPTGALMSGSFLKEVVRIAERSGAWILCDEVYRGLEHDPGVSTPSIVDLYERGVAT
                     GSLSKVFSLAGLRLGWVAGSSGLIRECFIRRDYTTISCGMIDEMLALRAFSFKDRILG
                     RNLPLVRKNAVLLDNWVRSEKTLSYVRPKAGTTAFIRYGFPLPSEDFCRGLFEFNGAF
                     LVPGKAFDFEGWFRMGFSCSTETLEKGLNAVSDYLKALEKTTAGTQGKSTLL"
     gene            23542..25041
                     /locus_tag="PLY83_00755"
     CDS             23542..25041
                     /locus_tag="PLY83_00755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOV28598.1"
                     /translation="MIERGDFITPTLNYVKYFEKPVLHYWLTALSFKLFGYSEFASRF
                     WPALLGVLGTGLTFWLASALYGTRAGFFSGLILATSWIYFAIAQINIIDMGVSVFITM
                     ALAGFFLGSTGDRRNLLLFYGGMGLATLSKGLIGIVLPCAVAGTWILATRNWKLIRRT
                     LYGWGILLFFGLTLPWYIAVCLKNPEFFHFFFIREHFLRYTTKIHGRYEPAWFFIPIL
                     LAGGLPWTAFWIRPLAGLRESREKLFLFLWFAVIFSFFSFSSSKLIPYILPVFPALSI
                     LAGDKIDRYLKQPERETIKFELLLSSILIGLFSAALIAYPFLQDRFPVTVLLRVTVPL
                     ATTLLLGLLCAWTSWMKRRPQALVATLFIGAAFACLALKGGFAFYGSFLSAKQTADII
                     EKARRPGDLIVSYEDYDQGIPFYLKTRVVLVNWKGELEFGSGIGDQSEWFISREKFMD
                     LWKSNRHMIVIFRENRYRELLSKGVGNMHVLGRANGAVVVTNRPEGETQ"
     gene            25038..26225
                     /locus_tag="PLY83_00760"
     CDS             25038..26225
                     /locus_tag="PLY83_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HOV28599.1"
                     /translation="MRRDTFLPFSRPSIDELTIEDVCESLRSGWITTGPKSIRFENAF
                     RECVGTPYALAVNSATAGLHLAHLALGIGPDDEVITTPMTFVATLNTIVLAGARPVLV
                     DIDPGTFNMDPGRLESAVTSKTKAIVPVHFAGLPVDLDAINEVARRHGLAVIEDCAHA
                     LGASYKGRTIGSDPGHISVFSFHPTKNITTGEGGMVCTGLEEVAERIAILRQHGMSKG
                     AWSRYAASGTPHYDVALPGLKYNMLDIQAAIGLGQLSRLDAFNARRASIVERYKSAFS
                     GLEGLVLPATPSYEHVHAWHIFTPLLDLDRLDLTRDEFMSLMKEANIGTAYHYQAVHL
                     FDFYRKTFGFKPGDFPNAERISERIVSLPLFPAMTDEDVEDVIEAVTSILKSRLRRGR
                     GKA"
     gene            26222..27160
                     /locus_tag="PLY83_00765"
     CDS             26222..27160
                     /locus_tag="PLY83_00765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOV28600.1"
                     /translation="MTSPHLSVVIPVYNEEESLPLLFARLMPVLENLNRPYEVVFIND
                     GSRDRSLALLLDCRERYPGRVVVVDFNGNFGQHMAIMAGFSQARGEMIVTMDADLQNP
                     PEEIPRILAEMEAGHDVVGTVRSGRQDPLFRKFASKIVNRISNRITGLKLNDYGCMMR
                     GYNRRIINFINQCKETTTFIPALAQKFAMNPVEIPIAHSEREQGQSKYGLYRLIRLNF
                     DLMTGFSLIPLQAVTMLGIIVSILSFLFSVFLLLRRLVVGPEVEGVFTLMAISFFLMG
                     ITLFSVGLVGEYIGRIYQEVRERPRFVIRKVWNHEG"
     gene            27150..28088
                     /locus_tag="PLY83_00770"
     CDS             27150..28088
                     /locus_tag="PLY83_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase"
                     /protein_id="HOV28601.1"
                     /translation="MKAEPRVLLAAYSEVGVVCLEELLAQKAQVVGVFTHADDQFEEI
                     WFRSVARTAAEAGIPVFMPENINAAEEIERIRGLRPDILFSFYYRKLISKEILDIPQL
                     GAYNMHGSLLPRYRGRACVNWAVLKGETQTGATLHVMTEKADAGDILDQEAVPIEFTD
                     TAQDVFWKVRDAARKVLARTWPLLREGRAPRTPQDESLATTFGRRRPSDGLIDWNRSA
                     LELYNLVRAVTHPFPGAFSFIGERKWFIWKAWPLESGGKGQEGKVLGLDPLVIGTREG
                     SLRIDRCQAEGEPETDGADFASRSLRPGQILGGKTN"
     gene            28085..29110
                     /locus_tag="PLY83_00775"
     CDS             28085..29110
                     /locus_tag="PLY83_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HOV28602.1"
                     /translation="MKILILGVNGFIGSHLAERILNTTDWEVRGHDLRTTNLHACLNH
                     PRFTLRQNDMREDWEWIEAQVSQCDVILPLVAVAQPKKYVEDPLFIFELDFEENLKIV
                     RLCSRYGKRLVFPSTSEVYGMSQDEEFREDESNLVLGPICKTRWIYSCSKQMLDRVIA
                     AYGMQKGLQYTLFRPFNWIGPRLDDPSLAAEGKSRVLTQFIYNLLEGLPIQLVGGGRQ
                     KRCFTDIEDGIDALMAILKNEGSVADGQIFNIGNPRNNHSIRELAETLQRVLATFPGY
                     EEKARNALILDTPSETYYGEGYEDVEYRVPSIEKARRLLGWEPRIPLEETLRKTLSAA
                     IGQERPA"
     gene            29107..30012
                     /locus_tag="PLY83_00780"
     CDS             29107..30012
                     /locus_tag="PLY83_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOV28603.1"
                     /translation="MSITLGLKLDVDTLRGYREGVPALLDLFEKLGIRASIFFSMGPD
                     NSGKAIRRVFRKGFLSKMRRTRAVSTYGLKTMLYGTLLPAPLIVDSDPSILRRAAELG
                     HECAVHAWDHVEWQDCLDRMRIEAVRDALRRAFDAFERIAGQPARSCAAPAWKVTESS
                     LEVQDELQLEYCSDTRGQGPFLPVLGNRRFSTPQIPSTLPTLDEILGLDGMTPEESNR
                     RLLDGLREGINVHTFHAEMEGIGQIGLFEEFLVECKNRGVRFCTLGEIARFRSREELP
                     LCFLETGEIPGRAGKVALQGKEWRP"
     gene            30009..31343
                     /locus_tag="PLY83_00785"
     CDS             30009..31343
                     /locus_tag="PLY83_00785"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOV28604.1"
                     /translation="MKIAIIGTGYVGLVTGSCLANFGNEVLCVDVDKEKVESLNAGKV
                     PFFEPGLGDLLSPNLSAGRLAFTTDLDSAVKGAEIIFITVGTPPGPTGEADLSTVWEV
                     ARSIGSSLEQGSIVVTKSTVPVGTTFKVRTMIEEELQRRGKTVPFEVANNPEFLREGS
                     AVLDFMNPDRVVIGTESEKARESLAKLYSFLDEEKLLFMDILSSEMTKYAANALLATK
                     ISFMNEIAGICQNVGADVEKVRIGIGSDARIGFAFISPGCGYGGSCFPKDVRALIHTA
                     ASFGCKSRILEAGEKVNNAQKHLLFKMIAHHFETTAGRTIAIWGLSFKPNTSDTREAS
                     SLVLIEDLLVAGARVRAHDPVAIPEARKALPHHDNLEFCENHLEAVRGADALVLVTEW
                     DLYKQPDLARLAALMGSCVIFDGRNQYNPQEMKRHGFTYYSIGRPAVLGRRG"
     gene            complement(31340..32392)
                     /gene="corA"
                     /locus_tag="PLY83_00790"
     CDS             complement(31340..32392)
                     /gene="corA"
                     /locus_tag="PLY83_00790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HOV28605.1"
                     /translation="MPRDFRAGFYRRVSEKAPQESRPKPPRLGLITYTNERFFEKADC
                     SLEECLASREAEGIRWINVDGHSDPALLERLMEAFGIHPLVLEDVLALEQRPKLEDYG
                     EYLFAVLNMLTLEKRTGRTVSEQVSVILGKGFVLSVQEDPGDVFDAVRDRLRSGKGKL
                     RKMGSDALAYSLFDAVVDGYFIVLESLSDRVESLQDELVSSPNRETLQALHGLRSELN
                     YVRRCVWPLRELIGNLERSDSSLLDEALAPYLRDLYDHTVQVLESVESLRETLSGFLD
                     IYLSSLSNRMNEIMKVLTVISTLFIPLTFIAGVYGMNFRFMPELEWRWGYPAVLGVMA
                     GVAAVMLRYFRRKGWI"
     gene            complement(32406..33176)
                     /locus_tag="PLY83_00795"
     CDS             complement(32406..33176)
                     /locus_tag="PLY83_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOV28606.1"
                     /translation="MRMFWVRFEKEGRARSGLLEDGTVREIRGSFFEGGEFSGEVFPL
                     EEVRLLAPVEPSSIFCVGRNYTEHAKELGHDLPEEPLIFLKAPGSVLNPEGTVRIPLW
                     AGRIDYEGELAVVIGKTCRNIPEDEAQSVIFGLTCLNDVTARDLQNKDGQWTRSKSFD
                     TFCPLGPWILQSGETRGRRIETRLNGRVVQAASTDMMIFSVGRLISHISRFATLRPGD
                     VVATGTPAGVGPMLPGDVVEVEIEGIGILKNRVAADPS"
     gene            complement(33208..33597)
                     /locus_tag="PLY83_00800"
     CDS             complement(33208..33597)
                     /locus_tag="PLY83_00800"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - diacylglycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HOV28607.1"
                     /translation="MGSNPNTKCCWKNDSLARKFMNSLNGLKTAFFMETAIARETACA
                     VILTALAVYAGKGEFWLAFQVFLLSLVPMVIELINTSLELLIDSHVGTEFNEDVKRTK
                     DMLSASVLLALLVSYGAAAALIVPNWR"
     gene            complement(33587..34405)
                     /locus_tag="PLY83_00805"
     CDS             complement(33587..34405)
                     /locus_tag="PLY83_00805"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOV28608.1"
                     /translation="MNERNVWTVNSMQQIREWFASYTRSFQKGDNPLEPLLDLKLRHS
                     FRVAGEAGFLAREMGLDARSVDVAETLGLLHDVGRFPQFREFRTFYDAASVDHGELGW
                     ETVKRAGILDPLEDEASLAILDGIRFHNKKEVPSGIRPSSLTMVRLVRDADKLDVFEL
                     VQDYLDRGTIHELLPRIPDTGGISGELLDTLGETGRASYSQVRSVPDFLLVQLSWVFD
                     LNFQPTFRRILERRVIERIENKLPRIQQVVRLAGKARDFVRASAMAGEGTLDGE"
     gene            34486..34974
                     /gene="gpt"
                     /locus_tag="PLY83_00810"
     CDS             34486..34974
                     /gene="gpt"
                     /locus_tag="PLY83_00810"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HOV28609.1"
                     /translation="MDSSERYKKTFPISWDQLHRDCKALAWRLHEKGRWNRILAIARG
                     GLVPAAIIARELDIHFIDTLCVSSYTLRTQGEPAILKLATSFGNDPQGSLIIDDLVDT
                     GRTAQLVRRMFPEACFATVYAKPEGRPLVDLFITEVSQDTWILFPWDSEFSFSQPIIG
                     RT"
     gene            complement(34971..35900)
                     /locus_tag="PLY83_00815"
     CDS             complement(34971..35900)
                     /locus_tag="PLY83_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOV28610.1"
                     /translation="MEILIPILSAAIRSGTPILYATLGEILTEKAGVLNLGLEGLMLL
                     GAFSGFSATMVTGNPWVGLAAAVCVGMAVSALHAFLCVTMGANQVVSGLAITMFGTGM
                     SALLGKAYVGETIRGLGILPVPVLAKIPLLGPVLFNHDPLVYLSYVLVGLACWFISST
                     RWGLTLRAVGENPRAVDAAGLSVARLRYAYTMVGGGLAALGGAYLSVVYTQMWVEMMT
                     AGRGWIAVALVIFGIWNPLRAAFGSYLFGGVGALQLRIQAAGTTVPAPLLLMLPYILT
                     IVVLVGISVRKGKGILLGAPASLGQPFHREERD"
     gene            complement(35906..36976)
                     /locus_tag="PLY83_00820"
     CDS             complement(35906..36976)
                     /locus_tag="PLY83_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOV28611.1"
                     /translation="MKPRLRIERRLDVPSWMNWSIPAGAVGVALAAGAVLLSLLGVSP
                     LAAYKAMIQGSLGDAWGISETIVKAIPLTLAGLAVGLAFTMVVWNIGAEGQIVMGALA
                     AVAVVRYAFVDNSLVMFSLMFLMACLAGGLWAGFAGFLKARWNVNETITTLMMNYIAI
                     LLADYFIYGPWRDPSSLGFPMTTPFPEAARLPVLGGTRVHVGIFLAVGLAILFRVLYR
                     WTKWGFELRVIGNNPNAARYAGINDLKNVVLAMFLSGAVAGIAGMTEVAGLQGRLQPG
                     FSGGYGFTAIIVAWLARLNPMATILVSFLLGVLLVGGDTLQVVMRLPLASVQVLQGLI
                     LFCVLGAEFFRNYRIVFIERGE"
     gene            complement(36973..38526)
                     /locus_tag="PLY83_00825"
     CDS             complement(36973..38526)
                     /locus_tag="PLY83_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV28612.1"
                     /translation="MESGKQQPLVQMLGITKSFASVKANRGINFDVFPGEVHTLLGEN
                     GAGKSTLMNILNGLYQPDAGSIRIKGAECRFSSPREAIAAGIGMVHQHFMLVPVLTVW
                     ENLVMGLPGLKAVLPKREVIRKIGELSDRYGLDVEPEAQVWQLSVGEQQRVEILKMLY
                     RNAEVLVLDEPTSVLTPQEVRRLFDTIRQMTAEGHGVVFISHKLDEVMEISDRITILR
                     SGEVVETLPGPMATREMLAEKMVGRKIVFAVDKAVLNPGDTVLEAEGICALNDRGLPA
                     IRNLSLSIRQGEILGIAGIAGNGQKELCEALVGLRPIQFGRVLLRGKDMTGRKPMDFL
                     RAGVSYIPADRKGTGLVGAMNLRENFALKNFWRKPYAKGPFLSWESIAGETGRIVERF
                     GVSAPGLGIPVRNLSGGNQQRLMLGRELSGTPDLIVAMQPTWGLDVGATEFVRDQLLR
                     QRERGAAILLVSEDLEEVLALSDRLAVIHKGEIMGLVEDPRSASEEELGLMMAGIRRE
                     TAIEGEKGR"
     gene            complement(38650..39732)
                     /locus_tag="PLY83_00830"
     CDS             complement(38650..39732)
                     /locus_tag="PLY83_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOV28613.1"
                     /translation="MRRGLGFAFVMAFLLAATTAWAFAPVPAKDLKPGFVYVGPVGDG
                     GWTYMHDKGRKDMEKALPGVTSSIVESVPEGPDAVRVMETFVRNGSKLIFATSFGYMD
                     YVQEVAKKHPDVVFMHCSGYKTAPNVGTYFGRIYQPRYLSGLVAGKMTKSNIIGYVAA
                     NPIPEVIRGINAFTLGVRKVNPKAKVKVVWLFSWFDPGKEKEATKALIDSGADVIAMH
                     ADSGAAPQAAEEAKVWVVGYNNDMSRYAPTRHLTAPIWNWGMLYKYAAENVIKGTWKS
                     ESIWWGLKENMVGLAPFGKAVPESVRKLVAEEKGKIVSGKWDVFTGPIKDQSGKIRVE
                     AGKRMSDPEMLSFNWFVQGVEGTIPK"
     gene            complement(39868..41412)
                     /locus_tag="PLY83_00835"
     CDS             complement(39868..41412)
                     /locus_tag="PLY83_00835"
                     /inference="COORDINATES: protein motif:HMM:NF024941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4127 family protein"
                     /protein_id="HOV28614.1"
                     /translation="MSRFKKPLLRLLTMVLLLPFVARPVFAGLVVYVPLDDRPYSLDR
                     VIEAARALKVELLLPPAGFFRGQAGVSDYEELFRWLASNSSQGAFVIAADALLFGGLV
                     SSRIHDLDEEEVGWRFEALKKFCDSLQKPKIALFGTLLRMPRESQGVLDADYNAKWAP
                     QLFRLARLQTAASRGELSKEELAAAERLLKTIPVPVLREWAERRNRSLRAANRLLALH
                     GAAGVDFVGIGLDDNVCPQLLEPEFRSMASVIGEASRDRAAVVPGVDQMGLLLFARMV
                     LHDKPRQKVCLLFPRSFGEKLQPNYSGFTPEESFEIQADVLGVERVATPQEADMVLVV
                     NLPQGERTLEASSPANNATPDESDEAFAGEIGRILDTGKRVAVADIAFDNGADRGLME
                     ALRKEGLLTRLAAYSGGNTSDNSIGVALATGVLSAWMTEEDRKWLLFTRYLDDWGYQA
                     VVRPAVVPKGPFAGQAVAGRLEGEIREKLQAFSQGPLADFGFADFEVSYPWSRLFEIR
                     ITREKP"
     gene            complement(41474..41549)
                     /locus_tag="PLY83_00840"
     tRNA            complement(41474..41549)
                     /locus_tag="PLY83_00840"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41514..41516),aa:Asn,seq:gtt)
     gene            complement(41640..42380)
                     /locus_tag="PLY83_00845"
     CDS             complement(41640..42380)
                     /locus_tag="PLY83_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV28615.1"
                     /translation="MSHHFLEVRDLWVAYPDGTEALRGLSFRLEHGEAVGLVGANGAG
                     KSTTLLALAGLINPCRGSVNLGGTVLSKQTVGEIRRRIGFLFQDPDDQLFMPTVFDDV
                     AFGPLNQGLTAEEVEERVRTALEQVGARHLAERPAHRLSGGEKRSVAIATVLSMMPDV
                     LIMDEPSASLDPRGRRLLIGLLKSFTHTRLVATHDLDLVLEVCPRTIVLSGGRVEADG
                     ETARIFQDRTLLERCGLEPPLCLQGMPR"
     gene            complement(42377..43168)
                     /gene="cbiQ"
                     /locus_tag="PLY83_00850"
     CDS             complement(42377..43168)
                     /gene="cbiQ"
                     /locus_tag="PLY83_00850"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HOV28616.1"
                     /translation="MPTSGCSLDENPGFFSRGGVDPRAKWLVTFAFLLVVTSFGKYEI
                     SRLTPLVLYPVSLTAVSCVPVTKLLRRVLVALPFVLFVGLFNPLFDREILFKVWGLSI
                     SGGWISFVSITFRSLLAVAAAVVLAMTTPFDRLCLGLEKMGVPRVLVTQLQFLYRYLS
                     LLQEEAVRMVRAHALRARGNRPKIRVSSAASMIGFLLLRSLDRATRIYRSMLVRGFDG
                     EVRLMKPLRFAWSRDGIFVGFWTLYFLTVRFVDVPGRLFGWVLGR"
     gene            complement(43178..44236)
                     /locus_tag="PLY83_00855"
     CDS             complement(43178..44236)
                     /locus_tag="PLY83_00855"
                     /inference="COORDINATES: protein motif:HMM:NF014007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HOV28617.1"
                     /translation="MHMADALLSPAVGGTMLAVTAGLTAYSARKLKESPDESRIPLMG
                     VLGAFIFAAQMINFSIPATGSSGHLGGGMILAILLGPYAAFLVLASVLVVQALFFADG
                     GILALGCNIFNLAFFPCFVAYPFIYRKIVGDSPSRKKILLGAILSAVAGLQLGAFSVV
                     LETVASGISELPFRTFLLLMQPIHLAIGIVEGIVTGAVVTFVWKARPEVLESAARNTP
                     LPGVSLRKVLAGLALAAIFTGGALSWFASTYPDGLEWSMAKTAGTEELEAPAERIHTA
                     LAALQEKLAFLPDYGFRPSPAGKDSQPEAAAAGENEGKWPAVDPATTVSGLVGGALTL
                     TLAGGLGILLRGAVSRRK"
     gene            complement(44353..46032)
                     /locus_tag="PLY83_00860"
     CDS             complement(44353..46032)
                     /locus_tag="PLY83_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOV28618.1"
                     /translation="MEQAEPKSGNFIEQIIREDLESGRIKKVVTRFPPEPNGYLHIGH
                     AKSICLNFGLAEKFGGDCNLRFDDTNPAKEEIEYVESIKKDVEWLGFRWSNLCFASDY
                     FDQFYLWAIELIKAGKAYVDHQSPEEIRKTRGTLTEPGTESPWRNRTVEENLELFEKM
                     KNGEFEEGECVLRAKIDMSHPNVNMRDPVMYRILKESHHNTGDRWCIYPMYDFAHGYE
                     DAIEGVTHSICTLEFQDHRPLYDWFLDNVSVPHRPRQYEFARLNLTYTVMSKRKLLEL
                     VTTKCVSGWDDPRMPTISGLRRRGYTPSSIRRFCEEIGVAKTNSMVDIEFLQFCIREE
                     LNKTANRVMAVLKPLRVVIENYPEDRVEEIEALDNPESETPTTRKIPFSRVLFIEQDD
                     FMENPPKGYFRLSPGKEVRLKHAYLITCKSVVRDEEGRIVELRCEYDPATRGGDAPDG
                     RKVKGTLHWVSASHAVEAEVRLYDHLFTLKNLDEVEEGKEYRDYLNPDSLVVLSGCRV
                     EPGLSRVEAGQRFQFLRHGYFCVDPDSRPGRPVFNRIVPLKDSWAKEQKKG"
     gene            complement(46084..47700)
                     /locus_tag="PLY83_00865"
     CDS             complement(46084..47700)
                     /locus_tag="PLY83_00865"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HOV28619.1"
                     /translation="MNGLFSPDHYHFQACSALFLISGLLASGFLSFVRRKRIGGAGVY
                     LLLLLWAVAHWLFAQVLESAAATAGLQLFWRQVATPAIASCPVFFFLFALRYALAGNF
                     PTRYWSSLLFALPGITVFLAITNERFHGLWSEILLNPETGRLFFEPGPWFWVHVVYSS
                     VLLFAGALLIVKRALQVSRHAMSQFFVLLAGSLAPLVVNASSFLWGTLGQTPDWMPLA
                     FLFSSGMLTVGITRLGILDLVPVARSLLVETMPDGVLVVDPRGGIADLNPAMASILGR
                     DRGEIYGMTMEEAFRDWWGISGFFDGDGEGLAEVERVLPDGGKLAYEVKVSTLRDRRA
                     GGRPAVMGKLFVFRDASLRRKVEEQLREAAVVDPLTGLMNIRAAYGLLEELVKGKRSD
                     GTIFSLALLDLDHFKRINDTLGHRAGDDVLEKFAALVRKSSVPGQVSARYGGDEFLLV
                     FSDYSKREAEILLLGLLEEVRLSIRAAPGRKLSMGFSAGLVDTEEFSTLSELTVEKML
                     EVADERLYQAKAAGRNRVCADSPIGTRSNL"
     gene            complement(47710..48048)
                     /locus_tag="PLY83_00870"
     CDS             complement(47710..48048)
                     /locus_tag="PLY83_00870"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOV28620.1"
                     /translation="MNGLRYCVYLLRCGDGSLYCGITTDLQRRVREHREGKGARYTAA
                     HLPVELEYATSCWFNRAEAQAVERRVKSRRRGEKKSFLEEVERARFERESERTETAGK
                     GGFPNQGWIV"
     gene            complement(48045..48965)
                     /locus_tag="PLY83_00875"
     CDS             complement(48045..48965)
                     /locus_tag="PLY83_00875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HOV28621.1"
                     /translation="MENVLFLGMGAVGMTFASQFADRGYPFHFLCDRIRKEKYADRER
                     FVNGRLYAFPCLMPSEISVPCDFVFVSVKAYQLQDVLPLLEEAVGPQTVVLSLLNGID
                     SEEVIAARLGADKVLPSFVARMDSTREGDRLVYSSPGQIVYGEADGTFSPRMKRLAGL
                     LEEAGVRYEATGEIRKKMWWKFMVNVGINQTGTILKAPYRVFQEVPQSIEAAFAAMRE
                     VMALAPYSGVSLTEEDLEESLLMLRQFNPEGKNSMLQDLEAGRKMEIETFAGKVCRLG
                     EQHGVPTPVNRLYYNLLKTLECQFASDRSE"
     gene            49042..49500
                     /locus_tag="PLY83_00880"
     CDS             49042..49500
                     /locus_tag="PLY83_00880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOV28622.1"
                     /translation="MVGRIECCLKDAKPEAKRGGRSMQNSLEDYTRIFDALLQNSQDA
                     VALCDTDQVILKINRAFTELFGHTEEEAVGKRLDLLVATTPEMMASSDMQVDRIRIWE
                     KVFTQAVRRTRKDGSTVPVSIEGNVFRLANGERLILWNYREIPAEEGPGN"
     gene            complement(49523..49765)
                     /locus_tag="PLY83_00885"
     CDS             complement(49523..49765)
                     /locus_tag="PLY83_00885"
                     /inference="COORDINATES: protein motif:HMM:NF016141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HOV28623.1"
                     /translation="MSFLGFVFVGLLAGWLAGHLVKGGGFGLVGNIGVGVVGALIGGF
                     IARNVGLSFGSGFWGNLLIATLGAVVLLVLIRLMRK"
     gene            complement(49775..50728)
                     /locus_tag="PLY83_00890"
     CDS             complement(49775..50728)
                     /locus_tag="PLY83_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOV28624.1"
                     /translation="MKRILVTGANGQIGVELVPYLRRLYGDANVLATDCRAASQEMRD
                     SGPFELLDVRDGKAFLDLLSRFKADTVMHLAGILSATGEANPQLAWDVNANGTFTALE
                     CARSTGAAVFFPSSIAAFGPNTPPKMTPQDTLQRPATIYGIAKVTGELLCDYYHLKYG
                     LDTRGLRFPGLISYVALPGGGTTDYAVHIYYDAVSKGSYTSYIAKGTFMDMMYMPDAL
                     EAVVQLMEADPSRLVHRNAFNISAMSIEPEQIALEIQKHIPGFSISYEVDPVRQRIAE
                     SWPDSLDCSAARIEWGFSPKYDLASMTTDMLKRVREKLARS"
     gene            50927..51628
                     /locus_tag="PLY83_00895"
     CDS             50927..51628
                     /locus_tag="PLY83_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOV28625.1"
                     /translation="MDHQMELEELALRIKGDSSAPYYIANVLREAIYRGILEEGKPLH
                     QATLAQRLNVSPIPLREALRLLETEGLVTFRGCRGAVVTALSLEEARELYEMITALET
                     SLMRIAFPKITRRILEEAAAILEEMESEEDCIRWRDLNQMFHNLFYEPADRPLTLDML
                     ARLRQKTDRTIRVHLASMRIDSQKQHREILLAAESKDLEGTLAALSNHLTYTSNDLQS
                     CMRAEASTRGKPPQA"
     gene            complement(51625..52011)
                     /locus_tag="PLY83_00900"
     CDS             complement(51625..52011)
                     /locus_tag="PLY83_00900"
                     /inference="COORDINATES: protein motif:HMM:NF021462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2177 family protein"
                     /protein_id="HOV28626.1"
                     /translation="MPFFKVYLLTLLPFIGIDALWLGLVAPRFYKSQIGSILAPKPNW
                     FAAGIFYLLFVAGMVVFVTGRPGTPLQVAMRGALFGLVCYATYDLTNLATLQGWPVLV
                     TVVDLAWGTFLSTVVALVAHGLSRVF"
     gene            complement(52025..52609)
                     /locus_tag="PLY83_00905"
     CDS             complement(52025..52609)
                     /locus_tag="PLY83_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOV28627.1"
                     /translation="MKSLKGTKTAKNLMKSFAGESQARNRYSFYSSIAKKEGLIQISN
                     IFTETAENEKEHAKRFFKFLVKDLDGEAVPVEAAYPVALGDTKRNLLAAAEGEHEEWH
                     DLYPAFAQVAEEEGFAEIAAVFRLIAEVEKRHEARYRKLYSNLCSGTVFKQSEPVEWK
                     CINCGYIHKGDSAPETCPACAHPQGYFEVLSEKY"
     gene            complement(53130..54335)
                     /locus_tag="PLY83_00910"
     CDS             complement(53130..54335)
                     /locus_tag="PLY83_00910"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOV28628.1"
                     /translation="MPHFAERMKTMEKSARIIRNLFGAMGDPGLISFSGGAPAKEALP
                     IDIVREIAEDVLRSEKRGVEALQYGSVQGLPDLREVVVRELLAPKGIDAKAENILITS
                     GGLEGINLLCQLFIDPGDVILVESPTFVHCVEIFEMFQAKCISVAMDENGMIPEDLEE
                     KIRRYDPKVVYVIPTFHNPTGRTLSLERRKKIAEMGSRYDVVILEDDPYRDIRYSGED
                     LKPIKAFDETGNTVLANSFSKIFSPGSRLGYVLASDPVIEKLLDAKSATNSHTSMLPQ
                     VICAEFFKRGHYPAHHRMICDLYRTRRDAMLESIDLFFPAGTKRTFPDGGLFTWVELP
                     GRIDTTELLADATSNPDVKVAYVAGEGFFIEGGGMGKNCMRLSFGAVPPEKIRIGAER
                     LGKFLRSRV"
     gene            complement(54461..55567)
                     /locus_tag="PLY83_00915"
     CDS             complement(54461..55567)
                     /locus_tag="PLY83_00915"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HOV28629.1"
                     /translation="MSKKTLLVIDVQKDYFPGGKFPLWQAEETLVQVEKAICQAKTLG
                     IPVILVQHVVSSENSGASFFKEGTTGVEIHPKILAVAQGAPIVVKGNADAFLETDLEE
                     TLGRFKTAELLVCGMMTQNCVTHTAISRSAEKYAVTVLSDCCTTVDEKIHRIALNALS
                     GRVRLASWTEALAGDSGEKKQPGTSPAGPLRALSLPGFSIRPATGEDVPLILQFILEL
                     AEYEGLAHEVEATEDLLRKTLFGNPPSAEVIFACHEGIPVGFALFFQNFSTFQGRPGI
                     YLEDLYVRPAWRGKGYGTALLAFLAKMTVERNGGRLEWGVLTWNTPAREYYLSIGARP
                     QERFLLNRMEGEELKALADRFPEDAWLGGPVRSF"
     gene            55758..56162
                     /locus_tag="PLY83_00920"
     CDS             55758..56162
                     /locus_tag="PLY83_00920"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOV28630.1"
                     /translation="MKRKVLKSKLHRVTVTGADLNYEGSVTIDPLLMEKANIVEYEYV
                     DIWNVTYGTRFSTYAMAGQPGSGTICINGAAARLVSVGDKVIIATWQEIEEEEAKTHL
                     PRLVFVDENNVPVDPEREREIPGQAERKIASR"
     gene            complement(56257..56949)
                     /locus_tag="PLY83_00925"
     CDS             complement(56257..56949)
                     /locus_tag="PLY83_00925"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HOV28631.1"
                     /translation="MKKLGIVGGIGPESTLEYYKGIIQGFRKRTGEDRYPRMIIDSLD
                     LSEMYDYAAKKQWEPFIGRLVGSIKALAAGGADFAVIAANTAHIVFDEVRKDSPLPLI
                     SIVEETCKYAKSKSCERVVVFGTAFTMRSGLYTRAFEKYGIEAFVPDEQEQEAIHKII
                     FPNLEAGIVLEEEKRKILEIANRNLSLRKADALVLGCTELPLIIKREDLDVMVLDTTK
                     IHIEAILDSMLG"
     gene            complement(57038..58339)
                     /locus_tag="PLY83_00930"
     CDS             complement(57038..58339)
                     /locus_tag="PLY83_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV28632.1"
                     /translation="MKQPGTIRVAIAGAGAMGKGIAYQCRFTDGVQCVALADTRIQKA
                     IDVASWLGFEPVPIGRPSEVGAALSKGRMAVSEDTCVLAESPDVDILIDATSAISEAG
                     RFCELALKSGKDVIMMNAEADLIFGPYFLELAREKGVVYTSCDGDQHGVIKRIVDDIR
                     LWGFELVMAGNIKGFLDRRANPHSIAHEAEKRRLDCRMTTAYTDGTKLCIEMALLANA
                     LGLDVTTAGMTGPRLAEVREVLEAFDFRTLWERRQGVVDYILGADPGGGVFVVGYCEN
                     EYQRFMMNYYKMGEGPFYLFYRPYHLCHVEVGKTILETALLKKPLLAPYFGFRSNVYA
                     YAKTDLGKGARLDGIGGFSCYGLIESTLQDEERGVPICLAEEMILKRDIPKDGKITWE
                     DVDFDEERSDIAMYRKALECCSKESKKAGTSGVCPRTERPS"
BASE COUNT        12277 a        18244 c        16055 g        11828 t
ORIGIN      
        1 gggcggccgg gaaaggtcaa gaaaggcctc ggggtaatca ggggtccccc agaagacgag
       61 gcggatcccc aaccggctgg acctttccgc ttccttgctc ccccattgtt tctccctcag
      121 cccctctagt ttttcctggc atccttccga aaagcccaga tccttccagc aagccctgcc
      181 tccctccagg acttcttccg gcagcaaccc ccgcctggac cacccttcaa gatggcgggc
      241 atggaatcct tcacaggcat tgagaaccag gagggccgcc tcacgctccg tcaatgggat
      301 tcccccctcc ccggtaggcc agggcttcac agacatcctc gacagagact tccaggtcgc
      361 ccctaaggtc tgcgatcgtc cgggccacct tgaggaccct gctgatcccc cggccggaaa
      421 gtcgcaggcc tctcaccgcc tccatcagga aaggacggac tttccgggaa agacaaaggc
      481 ccgaacggac cagcctttca gggatctccg cgttgcagcc gaacccttgg gccttccacc
      541 tctcctgctg gattttcctg gcggccgtga ccttctcacg gatttcccgg ctggagggtc
      601 cctgccgtcc ctcgaggcag acgagttcct ccggcgtcag acgagggaca gaaacgtgca
      661 ggtcgatccg gtccaggatt ggacctgaca agcggtttct gtacttttcg agcgtgtggg
      721 ccgagcagcg gcagggttca acggggtccc cccaatagcc gcagggacag ggattgcaag
      781 cacagaccag cagcactcgc gcgggatagg tgacggaacc ggaagcgcgg ctaaccgtga
      841 tgatgccgtc ttctagaggt tgccggagcg cctcgagcag gtcccggtgg aattcgggaa
      901 attcatcgag aaaaagaact ccccgatggg ccaggctgat ctctcccggc cgtagggtgg
      961 tccctccccc gcaaagggcg atggcactgg cggtatggtg gacaacacgg tagggccgcg
     1021 tccggtcggg ttttgccggg attccgaggg tgcttttcac caggagggtc tcaagtcttt
     1081 cttcatcgct cagcggcggc aggatccctc tcagggcacg tgccaggagg gtcttccccg
     1141 agcccgggga ccccatcagc aggaggttgt ggtgtccggc agcggcgatt tcgagggcgc
     1201 gttttgccgc cgcctgcccc ttgatgtcgg ccagatcggg ctcaatcacc ttgtcttccg
     1261 tttccgcgct gctttccgtg acgggaggaa tccgggactc tccccgcagg tgggccagga
     1321 ggcttttcag gtccggcggg gcaaaggctt tcactccctt gacgagggca acctccctgg
     1381 cgttccctgg gggaaggaac agggggatct ccatcttccg ggccaggagg gccgcgggaa
     1441 ccgccccctt ggtggtccga agccgcccgt ccatcgcaag ctcacccagg aacagggccg
     1501 tttcaccgac tcgaaccgct cccgattcca tcgcaagtcc caccgccatc gggagatcca
     1561 ggagggatcc aaccttgggc aagtcggccg gggccaggtt cagcgcgatc ctccctctaa
     1621 gctggattcc gttgctcctc agggccgctc ggacccgttc ccgggcttcc ctgaccgctg
     1681 cgtccggaag ccccaccacg gagatgacaa acagtcctcc cgtgatttcg acctcgatat
     1741 cgatggggat cgcttcgatc cccttcaggg tcactccgag gatgggtccc atcagacctg
     1801 aaccctcccc acggtgatgt ccggcaggtg ggaaaacctg ggatgtcctt cctcgtccag
     1861 gttgattccc agaaggtcga tcctccaagg gccctgccac cggcatcgat cgacgtagat
     1921 ccggcccgac ctcacgaggg ccttcagctt cctgggcccc accgtttctt ccgccggcag
     1981 gattttcccg atcgaacgcg tccgaacttc gatgagaacg agttccggcc cgtccatggc
     2041 gacgaggtcg atttcgcccc cgcgcacacg gatgttccgg cccaggatgg tccagccctt
     2101 tgcgcgaaaa aagcgctcgg caagggcttc tccaagtcga cccgtttcaa ggtgatccaa
     2161 gacaacgcct cctcgagagg cccgtccggg aaaaggactc tggtgggcaa tccgctcagg
     2221 agaggatttt ccggcaaaac gaaatacggt ggatttcgca cgggccgtat tctttcaggg
     2281 cttccctgtg agccttggtc gggtagccct tgtggatctc gaaaccgtat tgaggaaaga
     2341 ggcaggccag ttgcaccatc acccggtcac gcaagacttt cgcgatgacc gaggcggcag
     2401 atacggcagg aaccagttgg tctgcccgcg ggatggcctt ctgccgaacc ggcagtcccg
     2461 cgatggggcg gttcccgtcc accacgacga gttcagcttc cgggtcgagc cccaggacac
     2521 aggccctcat ggcccaaagt gttgcgttca ggatgttcat cgagtcgatg cggccggcgg
     2581 aggccgcctg agctttccag cgcacgccga gttccagcat ggtggaaaaa agcgcttctc
     2641 tcctagaggg gggaagcctt ttcgaatcgt ccaggccaag cccgacgagg actttccgct
     2701 gcgccggcgt caggatgacg gctgctgcca caacgggtcc cgccaggggg ccccgtcccg
     2761 cctcgtccac tcccgcgatc ttcgtcaagg aaggttctcc tccgtcaaag gagattccgg
     2821 cagttccagc gtcaccctgc ccaggcgtcc cgaagcgaag ctttccagga actttcttgc
     2881 tgccgctcct gcatcgatgc ttcctcccga tacgaggcag cccagccggc ggccgagggc
     2941 atccagcgtc gcttccggcc ccgcggggtc caaggtcacc ccccaggcct tttcaacgac
     3001 cggccagagg ccctggtctc ccagaaaggc gatgagagcc aatgcgcagg attcgaagga
     3061 cccgatgaca tccgcccggt tacatccaag ccaggaaagg gcccgtccgg tcttcaaacc
     3121 ggttttagcc tggagaatgc caggcgagtc gacgaccagg actccctgtc cgcggaacca
     3181 ttcgactccc ctggtgatcc ctggaagggc cccgaccctg gaggcattcc gccccacgag
     3241 gccattcaga aaaagcgatt taccgacatt tgggattccg acgatcccaa ttcgcaggtc
     3301 gcgatgggaa ggcttgtagc gatgcagttc ggctttcagc gcatcaagcc ggtcctgcct
     3361 gagattgaaa gccagggcgg ttctgccgct cttcctgaaa ttctcgagcc atcggaccgt
     3421 cttcgcttct tctgcaagat cccgccggct caggacaagg accaccggct tcatcctggc
     3481 cagccgttcg gacaaaggag aggcagtaag ggccggggcc ctggcgtcac ggacctcgac
     3541 caccgcatcc aggttcgcga ccacctcgaa aagccgcttc tgtccgcggg ccatgtggcc
     3601 cgggaaccag accgttcgcc ccattttgct agttcagcag cccgattcgg gaaaggggcc
     3661 agtagcgcag gaaggccggt cccttgatga aagaccgcgg gacaaagccc cagaaacggc
     3721 tgtcctggga attcggccgg ttgtctccca tgacgaaata gtgtttttca ggcactttca
     3781 tctcgggcat attgaaatcg tcgtggttca ccacgtaggg ttcggaaagg gcctttccat
     3841 tcacgaaaac cgtcccgttc tccatgcgaa cggtttcccc tggcagggcg atgatccgtt
     3901 tgacgaaatc cctggatgga tcgacgggat acttgaagac gatgatctgc cctcgctttg
     3961 gttccgaaag agcgtgccag aacttggcaa cgaggacccg gtcccccggg tccagggtcg
     4021 ggatcatgga tccgctgggg atccagaaag cctggacgac aaaagccctc aagatcaggg
     4081 caaggacaag ggcccacaga atcgtttcaa tggtttctct ccaccacggc tttgccggca
     4141 tttctactcc tcctcctgga tccggaacgg tacaaggata cacccgttct tcccatcagg
     4201 gtaggctgat tcgaaccctt gcataggggg aaacgatgat cgagcggacc ggtgccctct
     4261 tcccgggggg caggattttc agggtaaacc caggggaaac cgaatggtag caagggttgt
     4321 agaccgccgt cacggctttc cccccggcca cggcaaagtc acattttccc atggaatcat
     4381 agatctcgct ctgcgtgaaa tcttgcccct gccagacgag gacaagcttt cccgacggac
     4441 ttgccggcaa tttcagcgtg aaggacaatt gcctgaggag ccccttctcg agggccacat
     4501 ccagccatcg ttgcaccgct tccgcctggc ggacgacggc cccttcggag cgttcccact
     4561 ggaccagggc ggtaatcgac acaccgactg caccggccaa tatccccatg atggcacaca
     4621 ccaccagcgt ttcgaccagg ctgaatccgc acgaagcgga gggaagcggc cgttccgctt
     4681 ccctccgctg tcggggtttc gcgtttcgtc tagttcccct tgaaataccc gtagcccaga
     4741 tccaccgcct tcaggttgtc ggccttgtgt ttctcgggtg ccagtttcgc cacggtctcg
     4801 cgaatctcgt cgatggaaac gttccctgcg ttaatcccgc acatcgcccc cagcatcagg
     4861 acattcatga agaggaaacg tccgcccatc ttttcgcgca ggatctggta accgggaatc
     4921 ccggcaattg acagactcct gtcggcggcg tacttcttca ggtgagggtt ctcgagggcc
     4981 gccgggtcgt ggagattgag gatcgcctgc gccccctcgc gaaggaaatg gaggttgcgg
     5041 atgccttccc cctggtcgaa agcgatcaga agatccgcgg aacccgccag aacgagaggg
     5101 ctctcgtacg gcccgacctt gaagtggctg atgacggacc cgcccctttg ggccatccca
     5161 tgaacctcgc ttcccatgac ccgctcgccc cgcgaaaccg cgatatgccc caggacccgg
     5221 ctcgtgaaga ggattccctg gcctcccagc ccaacgatga cgtactgcat cctttattcc
     5281 tcctctccct cgacggcctc gatggcgccg tgggggcaaa cctggataca gaccccgcac
     5341 tggacgcaga accgttcatc tatttttgcc tttttggccg cttcgtcaaa cggcaatcca
     5401 ggacagttga agaagttggt gcaatacttg catccaatgc atttgtccgg attcacgcgg
     5461 accttcacaa tcgcctgctt ttgcctcaac agcatgcagg gatggcgcag gatgaccaca
     5521 gcgggttcac ggtgagtcct ggcatgttcc caggccgctt tgaacacctc tgccgattcc
     5581 gtcgtgtcgt agggctcgac gaccttgacg aaagagaccc cgcagccccg gacgactccc
     5641 tcgatgtcca cctgtcggcc ttcgtcccct tttctcagct tgtccccgat gctcgggttc
     5701 gactgtccac cggtcattgc ggtgatccgg ttatccagga tcgccagaac aaaggcatgc
     5761 cgattgtaaa ccgcattgac caggccgagg atccccatgt ggaaaaaggt ggaatccccg
     5821 atcgtggcga cgacgggctg gtccttccca tccgtcttgt aggccaggta aaagcccgat
     5881 gccgaagtca gggaagctcc catgtcgatc gtcgtatcca cgtccttctg attgacaccg
     5941 agggtgtagc acccgatgtc ggagggatag atcccgttgg gcagggcttt cctcatcgcg
     6001 aagaaggagg cgcggtgggg acatccaggg cagagcatcg gaggcttggg cgtcaccttc
     6061 aattcctcga tggcggcctt caattctgag ttttcctcaa ccggcaaagg ttccccgagc
     6121 gccttcagga tcaggtcccg gatcacttcc ggcagaagtt ccccggctct cggaacaaat
     6181 ccgttccagc gccccttcag atgcgtccgg tccggaagct gcatttcgat gaccgggtaa
     6241 gtctcctcga ggacgaggac gttttcgtgc cgggcgatga agtcctcgac catcctcacg
     6301 ggaagggggt gcggcgtccc gatcttgaga acggagatat cctcccgttt ccaggccttg
     6361 agcagatcca tgacgatgga gaacgaaacg ccggcggcaa tgatgccaag ggaggcgcgt
     6421 ccccttgccg gcacttcgta gttgtagcgc ccatagaggg tctcgaaatc ctcgcggacg
     6481 gaatcgagct tcgcgttcag ctgcggatgg gtgattctca catgcgccgg gaggcagacc
     6541 cagcgccgga tgtccttctt gaaatccgct ttcctcccga gatccttcac ggcaccgagc
     6601 tccacgtcct ggcggcagtg atcgacgcga actgcggggc ggagcatcac gggcatgtgg
     6661 tacttttccg agaggtcaaa ggcatcaaaa accatgtttt tcgcttcctg agcggaggcc
     6721 ggatcaaagc acggaatctt cgcgaacatc gcgaaatacc ggctgtcctg ttccgtttgg
     6781 gaactgtgcg gacccggatc gtccgcggaa acgatcaaga aacctccggc aagatcgaag
     6841 tgagccacgc tcatcagcgg gtcggctgcg acgttcagcc ccacctgctt catgatcgcg
     6901 caggtccgct ttcccgtgaa ggaagccgtt gcggccacct cgaacgcaac cttctcgttc
     6961 accgcccact ccaccgccgt ccgggttccc agttcgtctg cgtatcttgc gaccgcgggc
     7021 aggatctccg acgagggcgt cccgggatac gcggaggcga cctcgcatcc cgcttccacg
     7081 attcccctgg cgatcgcttc gttgccaagc agaatgacct tgttacccaa ctgaaaccac
     7141 cccttcctcc ctggcttgac cgatgatttt ctgcagccac ccgaaaaacg gctcgggggc
     7201 cttgagcagc ttcccattcc tgtcgcagaa accgtgcttc gtctgccctt cggcgagcag
     7261 cagcccgtcc tctgaccgga ggatccggta gctaaacgtg acggtgtagg ggctcaggtc
     7321 agaaaggtca accaggacat cgaggagatc gtcgtagcgg gccgggtgct tgtagcgaca
     7381 cctcgcctcc accgcaggca gaaacacacc ggcttgttcc cattccctgt atggaagacc
     7441 tttcaaacgg cagaattctg ttcgccccga ctcgaaccag accaggtaat tcgcgtgata
     7501 ggccacgccc atggcgtccg tttccgcgta cctcacccgg atcctggccg ccggtcccga
     7561 tgagcgctcc tccatcctac cccacctcct tcctagccgc caacgattgt acaacaaaaa
     7621 gggggaaagc accccttccc cctcttaaaa ccttttccct ctgccaccgg gtcttttact
     7681 cccattccac cgggtcgccg gctgaaacga tgcccccctg aattacacgg cagaaaatgc
     7741 cttttttcgg catgatgcag tcgcccgtcg ttcgatagat ctcgcacttc gtgtggcatt
     7801 ccttcccgat ctgggtaatc tccagcaggg tttctccaac gcggatccgg cggccgaccc
     7861 tggattcgcc taaatcgatc ccctcaaccg ttacattctc ggcaaagctt cccggacgaa
     7921 gcccagggag tttctcctga gccttgcaga tgtcttccat cgcgatcaag ctgacctggc
     7981 ggtgagcgaa cccggcatgg gcatccccct ccagtcccag ctcttcgatg aggcggccag
     8041 tccgaatatc ctcctttggg atccctttcc gatctgaccg acacacggcc acgactcgcc
     8101 ccttactcac cgtgcccttc ctccattccc gggatgtcgt gatggcagac ggcatcgtgc
     8161 cgcggcggga gggggggaag cccctgcctc cggcggcggt aatcttcgat ggccgcacgg
     8221 atgccttctt ccgcaagcag ggagcagtga atcttctccg ggggcagccc tcccagggcc
     8281 tccgccactt cgcggttcgt cagtttcagg gcctcctcga tcgtttttcc cttgaccatt
     8341 tcggtcgcgg tggaactcgt tgcgattgca gccgcgcatc caaaggtctc gaaacggata
     8401 tcctcgatcc ggtcgtccct gacgcgcagg tagattctca tcacgtcccc gcaccgcggg
     8461 ttgcccactt ctcccacccc atccgggttt tccatcgcgc cgacgttttt gggattcatg
     8521 aaggcctgga tgaccttctc gttgtacatg gacatgcctt tcccctcccg gctaggcgtt
     8581 cttcttgaac ggcgacatgg cccgaagatt ttcaacgatc ggcggaaggg tattcaggat
     8641 ggtgtcgatg tcttcttccg tcgtatcctt ccccagcgtc agcctcagcg acccgtgcgc
     8701 ggtcgcatgg tcgaggccca tggccaggag gacgtagctc ggctcgagac ttccggacgt
     8761 gcaggccgac ccgctgctgg caccgattcc ggcgaagtcg agcctcagca gcatggcctc
     8821 tccctcgatg taccggatgc agaaactggc gtggaacggg aggcgttcgg tcctgtgccc
     8881 ggtcaggagg acgtcctcga tcctcgaaag gaccccgtcg atcagacggt cccggaggcg
     8941 ccgttccttg tcgatttcac cgtttgcgat gcgcctggcg gccaattccg cagcggctcc
     9001 gaagcccacg atcccggccg tgttttccgt gcctgaccgg agaccgaatt cctgcccgcc
     9061 cccatggacc aggggcgcga tccggacgcc cttccggaca taaagcgccc ccactccttt
     9121 cgggccatac atcttgtggg cggccatcgt catcaggtca acggggaggt ctctcacgtt
     9181 caggggaaga tgacccgccg cctgcacggc atcggtatgg aaaaggatcc ccttctcccg
     9241 gcagatctcc cccagttccc gaatcggctg aaccgtgccg acctcgttgt tcgcgtacat
     9301 cacggtcgca aggatcgtgt ccggccgaat cgcggcccga aaatcctcaa ccctgacgcg
     9361 gccttcggag tcgaccggca ggatcgtcac gtcaaagcct tccttcccga gccacttcac
     9421 cgtatccaag accgcatggt gttcaattgc gctggtgatc acatggcgtc cctttccgga
     9481 gaaggattcg gcgacgccct tgatcgccag gttgtcggcc tcgctgcctc cccccgtaaa
     9541 aagaatctcc cggggttcag cgttcaaaag acgggcaacg cttgcccgag cctcgtcaat
     9601 ggcggttcgc acttcccgtc cccatgcatg aacactgttg gggtttccgt atttctccga
     9661 gaaaaaggga agcatctttt ccaggaccga cggatccacg catgtggtcg ccgagtgatc
     9721 catgtagatt ctacgcaaat ccaagacctc ctagcctgcg gttgtataaa ttagggctgc
     9781 ctaatttatg gcctcatcct agcaactccg gtccggttcg tcaagagtcc caagtcgagg
     9841 ccgaatcgac cgttcaacag tgcgtccggt gagtcgccat caggcaggcg ccggaaacca
     9901 cggggaagcc ttccacctgg agagcctttt ccgcttcggc gttctcggca ccgggctgca
     9961 tccagacgac aggcctatag ggcatttcac gaagatcccg caggatgggt tcctggttct
    10021 tcgccccgac aaaaagcgcg accacatcca cgttctggga cagttccagg aggcttgccg
    10081 cgcaatcgag gtccagcact ttctgccccg cataggccgg attgacaggg aaaagccgga
    10141 agcccctttc cagcaggtag ctcatgaccg caaagacggg ccgctcacgg ttggacgagg
    10201 cgccgacgat cgcgatcgcg gagggtttac agacaaattc ccggatcact tcctctttct
    10261 ctttcgtcat tggaatactc ctcccgaatc caggatcagg gtctcgacga gaccgtcctt
    10321 ctcagtttca ggttccggta tttccctggt aaggcaaaac ccttatcgtt cctgggattg
    10381 acgggatttt tactctgagg gacagccgtc cttgctcgtt cggccggatc cgcttcaggg
    10441 agcttcgcac aacccaggaa ccaaactcat gtcccttgct gtccagaagc gaaacggcga
    10501 tccctggatc cacgacatcc cactcggaaa cgttcaggat ttctgcctcc acctggagag
    10561 aacctgtctt cgggtccagg gaccatccaa gcttcgtgat ggccagggaa acccccggat
    10621 caaccgcgca ccctgccagg caagggggcg ataccaccag caggcaaacc agggaaacca
    10681 accagaagat ccatttttta aaccgaaccg acatttccat ctctccttcc cttccaatta
    10741 gcttgttccg gcatgatttc agccaggctt tcgtcttgga gcgactcgat ggtataatcc
    10801 gcaattgcgg agggctggcc gagtggtcga tggcggctga cttgaaatca gttgtggggt
    10861 gacccaccag gggttcgaat cctctgccct ccgccagcga ccttccatgc ggcaaccgat
    10921 ggttccatta tagatgctgc cttcctgttc acagccggcc aaacttgcct taaactaggg
    10981 gaaaccgcct ttccggcgga aaacgggggc aagaacgcgt tgggcgattt tctcttcttc
    11041 gatctgacaa acgagccgtt cttcatcgct ccttcccccc ggggagcggg tgtttcaggt
    11101 gcccacgagc cggaaacagc ccgttcgttt gacgaactca ggcaactttt acccaaaggc
    11161 gcattccagg cccttctttt caggatccct cccgatgcca gcctaaaaga aaccgtttcc
    11221 aggcttgaag agatctgcac cgatgtgccg attcttttcc tctgcgacga aacgcaggcg
    11281 gaagaagcca gcaagcggat cggcaggggg ctccatggat gcatcgaccg gaaggggcta
    11341 acctgggaaa accttcagca ttcgatccag tccgcgtccg ctctccggaa tgtccttggg
    11401 gcgaactccc gcctgaagaa ggaacttgca gaaacgcgcc ttgacgtgga gttgcaccgg
    11461 aaagcctacc gccgcatttc cgaagatctc cggtcagcag tccgaaccat gtcgagcctg
    11521 ggagaactcc atcggctctt cttccatgcc caggacgaga aatccaccct ccggaacttc
    11581 tgccgggcca tcgtaaaggg tcacggatac caaatggcct gggtcgggag gatcctggaa
    11641 agcgaggaag gaatccggat tgaaccgatt gcccacgccg gagacgaaca gggttttttg
    11701 gccaccccct ttcccggaaa cattcccctt cgagggatcc tcgaagggca cccctgggcc
    11761 gtccaaaaca tccgggaaga ggcaaagcgt tttgagtggg caggcgaagc gctgcaacgc
    11821 cgttaccgtt cggttgccgt gatcccgttc tttcgccccg acagaaactc ccggatggcc
    11881 ttctgcgttt tcgacggaag ggaaggcagg ttttcggcgg accgtcttgc catgctggcc
    11941 gacctggcga agctgctctt gaggggactt tcccttctcg aggaaaagat ccaaaagcag
    12001 gcggatgacg agacgctgaa gctgatgaag caggccgtcg agacctccgt cagcggtttc
    12061 gtcatttttg gcctcgaaga aggcatccag tacgtcaacg aagccgcctt gcgcctggca
    12121 ggatactcgt cccaggaaga ggtgatcggc cgttcgtacc gcgattttgt cctggtgaac
    12181 gaaaagaccc cccagatcct ggaaacggtt tttcgcgggg ggaactggac gggcgagttt
    12241 gacctgatcc gcagagacgg ttctctccgg gaagtcttca gcagcgtgag cgccgttcaa
    12301 cgggaggagg aggaaacccg gttcgtcctg gtttcgttca ccgacatcag cgaaaagcgg
    12361 aaggcagagc aggctctcct tgaatccaga agactctacg aggccatcat cgaggatcaa
    12421 accgacatga ttttgcgctt cctgccggaa acccttgaaa tcacgtacgc aaacctgccc
    12481 tatgcccgct actacgggaa gtcgccgggc gaactcatcg ggcaatcctt ccgttgccga
    12541 tacacccctg aagaggaggg ccgaatccga gaacaccttc ggaagttgac cccgcaagaa
    12601 ccggtgaggc agatcgagga acgggtgatc catccggacg gggaaatccg gtggcagcaa
    12661 tggatcgacc gggggatctt cgactcggag gggaacctgg tcgaggtcca gggcgtgggc
    12721 cgggacatca ccgagctcaa gattgcgcag cagaacctgc tgcgggagaa aacccgcctg
    12781 atgacgcttt tcgacaactc ccccgacggg attgtgttct gcaccgcaga acaggtgatc
    12841 gaatctgcca acaaggcgtt cctcgagctt ttcggctttt ccgcccccga ggtcctcgga
    12901 agaaaaatcg acgacctcct gcacgaaccc ccggacgaac gggcggctgc gctagagctg
    12961 aaccttgcgt cccaggacgg ccgaccgaga tccctggaag ggacacgcat cgggaagaac
    13021 ggcgtaccgg ttttcgtttc cgtcaccgtc attccccgca aagaacctgg gggagaaatt
    13081 tcaggaacgt tcgccatcta ccgggacctc acggagagaa ggcagaaaga acaggccctg
    13141 aaaatcagct ccaccatcat cgagggaagc cccgtggtgg ttttccggtg gaaagcggaa
    13201 aagggctggc ctgtcgagta cgtctcagac aacatccggc aattcggcta cacccccgag
    13261 gaattgaccg ccccgggatt ccactacgcg aaaatcatcc atcccgagga tctcccacgg
    13321 gtcgaatctg aaaccgaggc cctcgaagcg aaggggagca gctcgttctg ccaccagtac
    13381 cgcattgtca tgaagaacgg agagcaccgg tgggtggtcg agcgcaaccg gcccgaaagg
    13441 gattcatcgg gtggcctcct ctaccatttc ggggtcgtca tcgacgacac ggaacggaga
    13501 aacgccgaga tggaagcgaa gaaaagccac gaggcgctcc agaaaagcct ccggaaactg
    13561 gagcgatcct tccttcagac catcgaggtc ctttcttcga cgactgaagc gcgggaccct
    13621 tacaccgccg gtcaccagcg aaaggtcgct ctgctttccg aagccctcgc ccggcggttg
    13681 ggcctgccgg agaaaacctg cgagggaatc tacctggccg ccatggttca cgatttgggg
    13741 aaaatctcaa tccccgcgga aatcctgagc aaaccctcga ggctgaacga tttcgaaatg
    13801 gccctgatcc gtacacaccc ggaatccgcc tacgagatcc tgaaaaacgt cgaagcccgg
    13861 tgggacctgg ccgagattgt ccggcagcac catgaacgct gggacgggag cggctacccg
    13921 agggggctgc gcggggaaga gatccttctg gaggcccgga ttctggcggt cgccgacgtg
    13981 gttgaggcca ttgcgtcgga tcgtccctac cgtcccggac tcggcctcaa ggctgccatg
    14041 gccatgctcg aggaaagcaa gggaagggaa ttcgacccgc aggttgccca agcctgcctg
    14101 gaccttttga gggaaggctt tgatttccag ggggagattg ctttcgctcc cgatccctgg
    14161 ggaaaggccg gcactccctg atggctgaag ggcacgcttc caagcgcttc ctgctgcttc
    14221 ccgggttggc cgcgctcctg ctcttttcgg tctggatctt ctctacggcg gtgcaggaac
    14281 caaaacagga aagaatcgat tccaaaggag gaagtacgat ggtcaagctt gaaacgagca
    14341 agggcgacat cctgattgcc ctggatgagg aaaaggcccc tgaaaccacg gccaatttca
    14401 tccagtacgt cagggacgga cattacgacg ggacgatctt tcaccgcgtc atcgacggct
    14461 tcatgatcca gggcgggggg ttcgaccggg aaatgaacca gaagcagacc cgtcccccga
    14521 ttcgaaacga ggcggaaaac gggttgaaaa acgaagccta cacgatcgcg atggccagga
    14581 cctcggatcc ccattccgcg acggctcaat tcttcatcaa cgtcaaggac aacgatttct
    14641 tgaacttttc ttcccgcacc ccgcagggat ggggatacgc ggttttcgga aaggtcatcg
    14701 caggtcggga cgtcgtggat gccatcaaat cggtcaggac agggaaccgg ggttttcacc
    14761 gggatgtccc gctcgagccg gtcctgatcc tcaaggcatc gatcgtcgaa ccctaggcga
    14821 acgggaaagg ggatcccgcc ccagaaaggc cgggatcccc ttcgtgccgc tagtttcttt
    14881 ccgggtgctc ttcttccgga accaggaaaa gaatccccag gggtggcagc gtaagggcaa
    14941 ccgactgtgc gaatccctga aacggcacag gttccgactt aaccccgccc atgttcccct
    15001 ggccgctgcc gccgtattct tttgcgtcgc tgttgagcct ttcacgccag aatccggaaa
    15061 cggggacgcc gatccggtag tcgtggcgcg ggactggcgt aaagttgcac accaccagca
    15121 ccggatcggg cctttctccg gggcgcttgc gcaaaaaagc cacaatgctg ttgtccacgt
    15181 ccctgaaatc gacccactca aaccctgacg gatcgaaatc cccctcgtgc agcgccggcg
    15241 tttccttcag aaagcggttc aaatccttta cccagcgggc cgtccccgca tttcccgggt
    15301 tttccaggag cgaccagtcg agttcccgct cgtggtccca ctcccgttcc tgcccgaact
    15361 cccctcccat gaaaaggagc ttcttgcccg gatgcgcccc ctgaaaaccc agcaggagtc
    15421 ggagattggc gaacttcttc cacggatccc cgggcatctt cccgtacagc gagcctttcc
    15481 cgtaaaccac ctcatcgtgg gaaaggggga gaaggaagtt ttcggagaag gcataccaga
    15541 tgctgaaggt cagccggtcg tgaaggtgct ttcggtagat cgggtctttc ccgaaatagt
    15601 ccaggatgtc atgcatccat cccatgttcc atttcaggcc gaaacccagc cccccgaggt
    15661 ggaccggcct tgtgaccatc ggccaggcgg tcgattcctc cgcaaccatc tggatccctt
    15721 caaaatcccc gtagcaagcg gcgttgagct gacggaggaa ttcgaccgct tccaggtttt
    15781 cccttccgcc aaaacggttc gggatccact cccccgcttt cctggagtaa tcgaggtaca
    15841 gcatggaagc cacggcatcc acgcgtagcc cgtcggcgtg ataccggtcc agccagaaag
    15901 cggcgctgct caaaaggaac gaacgcactt cgtttcgacc gtaattgaaa atcgcgctct
    15961 tccagtcggg gtggaaaccc tggcggggat caaggtgttc gtagagatgg gttccatcga
    16021 actgggccag accgtgcgga tcggtgggga aatgggaggg aacccagtcc aggatgaccc
    16081 cgattccggc ccggtggaga gtttcgacca ggtacatgaa atcctgcggg gtcccgtaac
    16141 ggcttgtcgg agcaaagtag ccaagggtct ggtaccccca ggaaccataa aaggggtgtt
    16201 ccatcaccgg caaaagctcg acatggctga accccatctc cctgacgtat tcagccagca
    16261 agggagcgat ctcccggtag ggcagggggc ttccatcccc cggggaacgc ctccaggacc
    16321 caagatggac ctcgtagatc aacatcggtt ccgtcagcct tccgcgggat gcccggcctt
    16381 ccatccaccc cccgtcttgc cagttcttct ccagatccca cacgaccgaa gccgtccccg
    16441 gcgggatctc cgaatagaaa ccgaacggat caagcttgtc ggggatttga atcccaccct
    16501 ttccccaaat gcggtacttg taccggtccc cctgtcgggc acccgggacg aatccttccc
    16561 aaatcccgga atctcgccag atcatttgca gcgggttttt cctgcaatcc catccattga
    16621 agtcgccgat gaccgaaacc ccttccgcgt ttggggccca gagagcgaag tgaacccccg
    16681 cctttccatc ctcttccgct agatgggccc ccatcattct gtaaagtctc cgatgcgtcc
    16741 cttccctgaa caaatacaga tcctgtgcgg aaaaaaccga tccgtgcagc gcttcgcccg
    16801 cggtttcttt cgtcatgctg attctcctcc cgctttcccg gcattccggg ggatcctcag
    16861 gaccccgcgc accggcaatt caacccggcc aggccggtcg ttccccttac acttcagatc
    16921 ttggaagtcc ttcccgaccg agatgattca agaagctccg atcctttgcc tttcgtcccc
    16981 ggagggggat gcttcttcct ccaccgcatg aaaacccctt caaggggaca tttcgccgcg
    17041 gcatccctcc aggtcgaaag tcagctttag agagcattct cttcttccgg ccgcttcttt
    17101 gccctttcaa ggacggccta ccaggccgcg tcgtccgagg accgcaactt ccctgcgaga
    17161 tcccgcaaag cggaccgttt caggcgcctt acctcaggga ctcgcggcgc cccctccgat
    17221 cctatttcca taatacaggt acggccttcc ccgcagatag aggcggagga atttcaggag
    17281 ctgaacgttc cgtcagttct gctgcatgcg gggaaaaagt cggcgacggc aaggcttcca
    17341 gggttaaccc gggccctggc atgggtccgg tacaggaaat ccttttttca tccgacccat
    17401 cattctgccc tagggccatc ggaacgagag gccgcaacct tcgacagagc gacctgtaaa
    17461 gaggttccgc cagggccgcg tcgtgcccat ctgcctcaac gtcagcacga acatggaccg
    17521 tttgcctggg actgggccgg acccctttgc cacaggtgcg cctcgaccct tgcgctgcca
    17581 acacgcgcgc gttctttcat ccattctccc cccatcttcc tgctttcgaa agaacccttt
    17641 tgaacaataa aacatcatcc cttccggatt gcggacgggg ggctttcccg ttttgatccg
    17701 tctgttggaa aggaatcgga tcattttgaa gcgttttaaa gctttataat aggtttttac
    17761 cccctttggg ggtggtgaaa gaaagagggg atccacatga acggatggaa cggaaaatcg
    17821 ctctggatcg atttgaccct ggggaagctg gaaacccggg aaatcccctt ccctctcctg
    17881 gagtcctttg tcgggggaag agggctgaat gaccgggtgc ttttcgacac gatcaaggag
    17941 ggagccaacc ctctcggacc cgaaaatgtc ctcgccctcg cagtcggccc gctgagcggg
    18001 acaattctct cttccaccag ccgcgtgcag gtcagcactc tttctcccct ttcggggatt
    18061 cttggagacg ggaatgcggg aggaaccttt ggtctttgca tgaagcggtc cggattcgac
    18121 cagtttgtct tcaccggttc ttcgccaaaa ccggtctacc ttctcgtcga cgggttgcat
    18181 gccgttctcg tggaagcttc ggacctcatt cagacttcca cctgggaaaa gaccgacctt
    18241 ttgaagcagc gccacgggcc gggaacttcc gtcgcggcga tcggccaggc gggagaaaat
    18301 ctggtccgct tcgcttcggt gatcatcgac cggcatcact cggctgcccg tgggagcggc
    18361 gcggtgatgg gttccaagaa cctccttgcc atcgccgtga aaggccaccg ggaaacgccg
    18421 ctggcggatc ctgccacctt ccggatcctg gccgaaaccg accgggattt tctaaggaac
    18481 gatcctttcc agcgtgatcc gatcgcccgc ttcgggagcc acatcggcat gatccgctgg
    18541 tttcccggca gccggaacaa tgcatgctat cttggacccg acgaggtacc ccggtccctg
    18601 cttccggaaa cccttaaaac atgggaggta ggacggactt cctgcgccgg gtgcgtcgtc
    18661 ccctgcaaag acgttttccg aatcccctcg ggaccctacg cgggcgagat cggcaaggcc
    18721 atggaacacg aatgccttca ctgcctggga accaattgcg gaatcgaaga tcccgtcgcg
    18781 ctgatgacga tggaaaacct ggccgacaag tacggcatgt gcgtgatcgg gctcgggaac
    18841 accctcgctc tcgccaagga tctcttcgcc catgggctga tcgggctgga agaaacgggc
    18901 ggactggacc tttcctggga aaacgcaggg gcccagatcg aactgatcca ccagaccgcc
    18961 ctccgccagg ggttcggaaa cctcatcgcg gaagggatgc tgaatctggc ccggatcatc
    19021 ggcggaaggg caatggattt ctgctaccac gtgaaggggc tgggccgggg tcaccatccg
    19081 gcggggatct tctcccttgc ccacgcgact tccacccgcg gggccgatca cctcaggggg
    19141 cgcagctggg ctttcgggga aaacgatccg gacatctggc cgatgctgat ccgggaagga
    19201 ttcatccctg ttgatgaccc ggtccgaacg cttttcatct cagaaagggc cgttaccctg
    19261 accgacatga tcggccgctg caagggggcg gtcaacaact gggcaagcgc ggttcccctt
    19321 gtcgcctccc accctcttct gaggggcgaa gcgctgctgt tgtcctcggc aaccggggtc
    19381 cctttcacgg aagcgatgct ggaaaaggct tctgatcgag tctatcttct cgaaatggcc
    19441 ttcaacgcga ggcagaaagt ccgacggcgc gacgaccggt tcccggtccg gtgggaccgg
    19501 atcgggtcag actgggccga gcaggagagg aaaaaacatg aagccatgct cgatcgatat
    19561 tacgccctga ggggatgcga ccccgagacg ggcatcccgg gacaggaaag cctcacccgg
    19621 ctgggactgg atttcgcggc aaaagccctg gaaagcatcg atcccggtgc gcaatgggta
    19681 gggcctccgc tctggtcatc cgaacgctac cctcgtggaa cccggaggtg ctgagttttt
    19741 aacgagggaa ggggagatcc cccttccctc aagcgatctc gttttgaagg gtctcgatcc
    19801 acaattttgc gaccgcggct cccgttgaac cggttaccca ggactcggac cctccgcccc
    19861 tggccatgag ctttggctgt tcgcggatga ctttccgcag gtcgattttc gcccgggccc
    19921 cgcgtgccag gatgaagctt ccctcggatc cttctccgga cggcaccagc accaatccaa
    19981 cggagtcggg gtctgactcg acaagggact tcagccccgg aaccgccatc tcaggaagaa
    20041 ttcccggcag gaccgagaca aagagggggc tgcccccgag ggtttccctc tcccagggta
    20101 gagaaagcat ccccttggct ttgtccaggc tcctcagtag cgcctgcctt tcctcctgca
    20161 gccggtcgta aaccttctcc agtttctcca gcgggcaact gacctttcgg agcaggacac
    20221 ggacgacgcg ggagtattcc ttgaggactc gatcccggtg aacggtgaac ttgacctccc
    20281 aatccggggc agtgcctcgg taagaggtta cgaggacccc accaacctgc gcggtggagg
    20341 aaacgtgcgt tcccgagcag gcgatgcaat cgaatcccgg gattgtcaca acccgaatgg
    20401 gttccttgcc gagccggtcc cagttggctt tcagcccttc gatctcctgg gcttcttccg
    20461 gaggaaaaag ccggatcgtg acgggcaaat ccctggcaat gatttcgttg gcctcatctt
    20521 ccgcctcgaa aagcaggggc cagtcgatct ggcggtccca tttcaggaaa acggtcgtct
    20581 cctcctcgcc aacggcgacc ttgtacaccg tcagcccctc gtggcggctc tccagtacac
    20641 gggaaaggat gtgctccccg gaatgcatcc tggaaaggag cgcctgcctg tccagatcca
    20701 cctcggccag gacagcaagc ccttccgcgg gctctccctg caggatcctg accgcaagga
    20761 cctcctcttc cccgtcgcgg aaacagtccg tcacttccgc ggaaaaccct gaagctgcaa
    20821 gccgcccctt atcgccgggc tgtcctccac cggacacatg gaagcggttc tcttccagga
    20881 tcaggcgggc ttctttcccc tttctcgaaa cgattcggag gatgcgtgtt tcgtaaccgg
    20941 tgtccaacgt tgtttccccc cttccacggg acattatacc catcccggca ccagccccgg
    21001 ggagtagaat ggaaccggat tctgaagacg gaggggagaa gagaaatgac ttccgatgtg
    21061 ttctttaccg gaacacggac aacctcaaga aggaacctgc tggaccgcct cgaggagctt
    21121 gttctggcag cagggctcga cgacctcccg ctgaagggcc ggatgacggc ggtgaaaatc
    21181 catttcgggg aaccgggcaa cctggccacg atccggccga actacgtggc caggctggtc
    21241 cgtatgattc gtgaacgggg agggaaaacc ttcctgacgg atgcgaacac cctctacaag
    21301 ggacagcggg ccaatgccgt ggaccatctg caggctgcaa tggaaaacgg gtacaacccg
    21361 ctctccgcgg gctgcccggt cgtgatcgcc gacgggctct gggggttcga ctaccgggag
    21421 atcgaggtca acctgaaaca ctttgcacgc gcgaaaattg cctccgccat cgccgaagcg
    21481 gatgtactca tctcgatgaa ccatttcaag ggacatgaac aggccggttt cggcggagcg
    21541 ctgaagaacc ttggcatggg ttcgggctcg agggaaggaa agatggccat gcattgcgac
    21601 tcccctcccc gcatccaatc ggccagatgc gtcgggtgcg gcgaatgcgt gagaaactgc
    21661 gcccacggag ctgtccggct gaccccggaa aggaaggcct tgatcgatca aaccctctgc
    21721 gtcggctgtg ggcaatgcgt cgtggtctgc cctgccgggg ccgcccagat cagctggggc
    21781 agcgcgaaaa ccctgggtga aaggattgcc gagtacgcct tcgcggctct gaaagggaaa
    21841 ccagcctttc acgtcaattt cctcatggac gtttctccaa actgcgactg ctggaaccac
    21901 aacgatacgc ccatcgttcc ggatatcggc atcgccgctt cgaaggaccc cgtcgctctc
    21961 gaccaggcct ctgtggacct cgtcaaccag gcccccgtgc tcccggggag tgcccttcag
    22021 aagaccgggg aacacctcga agacaagttc caggccctgc accccgatgc ggactgggag
    22081 cggtgcctcg ttcacgcaga ggcgatcggc ctcgggacgc gctcctacag gagggtcgac
    22141 ttgccttgag ttccggcagt agtcttttcg agggctttca ggtagtccga aacggcattc
    22201 aatccttttt cgagggtttc cgttgagcag gaaaagccca tccggaacca gccctcaaaa
    22261 tcaaaggcct ttccgggaac caagaaggcg ccgttaaact cgaaaagccc cctgcagaaa
    22321 tcttccgagg ggagtgggaa cccgtagcgg atgaaagccg tggtgccggc tttcggccgg
    22381 acataggaca gcgttttttc gctgcggacc cagttgtcca ggagaaccgc gtttttccgg
    22441 acaagaggga ggttgcgccc caggatcctg tccttgaatg aaaacgcacg aagcgccagc
    22501 atttcgtcga tcatcccgca actgatcgtc gtgtagtccc tgcggatgaa acactcccgg
    22561 atcaaaccgg acgatcccgc gacccagccc agacggaggc cggcaaggga aaaaactttc
    22621 gaaaggcttc ccgttgccac tcccctttca tacaggtcga cgatcgaggg tgtgctgacc
    22681 ccgggatcgt gctccagacc ccggtaaact tcgtcgcaaa ggatccaggc tccggaacgc
    22741 tcggcgatcc ggacaacctc cttcaggaaa gagccggaca tcagggcccc ggtcgggttg
    22801 ttggggttgt tcaggacgat cagcttgcac cggtcccccg ccagcttttc caggtcgtcg
    22861 aggtcaggca ggaaaccgtt ttcgggtaaa agccgcagga tcctgacatc ggcgccgagg
    22921 gcctcgggga cggaatacag ctgctggtag gtgggcttca cggcaacgac cctgtcgccg
    22981 ggttccacga gcgtgaaaag agccaggaaa ttcgcgccga ttcccccgtg ggccagcagg
    23041 atgttttttt catccagcgt ttcgtaaaga cgggagatat gcgccctcag atcgaaggcc
    23101 cccgggattt ccccataggt cagcttcctt gaaaggagtt cttccaggaa attctccttc
    23161 cccccggaaa gaccgaggag ttcttccacg gtcagggatt cgacgcacgt ctcggcgatg
    23221 ttgaaccttg ctttttcctc ataggcgttc atccactctt ccacaccaaa acgttcgatc
    23281 ttcaacgctc ttcccccctt cggccttcaa gaataccatg aacccgcgaa gaatggccgt
    23341 ttaccgcaac cctggctgtt ggtatcattc ttcgggtgat ccggaactct cccgtcccga
    23401 atggaggatc caatgcagaa aaaccgtttc cgtgccttag ccctgctttt gctgatttcg
    23461 tcgtttctgt tttttctttt cctgggaaat catcctctcc tggaaccgga tgaggggagg
    23521 tattccgaga ttcccaggga gatgatcgaa cgcggagact tcatcactcc gacgctgaac
    23581 tacgtcaagt atttcgagaa gcccgttctc cactactggc tgacggcact ttccttcaag
    23641 ctctttggct actccgaatt cgcctcccgc ttctggccgg ctttgctcgg agttctcggg
    23701 acggggctca ctttctggct tgcgtccgcc ctttacggga cccgggcggg atttttttcc
    23761 ggcctcatcc tcgccacatc ctggatctat tttgctattg cccaaatcaa catcatcgac
    23821 atgggcgtct cggtcttcat cacgatggcc ctggccggtt tcttcctggg ctccacagga
    23881 gaccggcgta acctgctcct cttctacggg gggatggggc ttgccaccct ttccaaaggg
    23941 ctcatcggca tcgtcctgcc atgcgccgtt gccgggacct ggatcctggc aacccgaaac
    24001 tggaagctga tccgaaggac tctttacggg tgggggatcc tccttttctt cggcctgacc
    24061 cttccctggt acatcgcggt ttgcctgaaa aacccggagt ttttccattt cttctttatc
    24121 cgggagcatt ttctccggta caccaccaaa attcacggca gatacgaacc ggcctggttc
    24181 ttcattccca tcctcttggc gggcggcctg ccgtggacgg cgttctggat ccgcccgctg
    24241 gccggcttgc gcgaatccag ggaaaagctt ttcctgttcc tgtggtttgc cgtgattttt
    24301 tcgtttttct ccttttccag ctcgaagctg attccctaca ttcttcccgt tttccctgcc
    24361 ctttcgattt tggcgggaga caaaatcgac cggtacctga aacagccgga gcgggaaacg
    24421 atcaaattcg agcttctcct ttcttcgatc ctgatcggcc tgttttccgc cgcactgatc
    24481 gcctatccct ttctgcagga ccgcttcccc gtcacagttc ttctcagggt gacggtcccg
    24541 ctggccacga cgctcctcct ggggctcctt tgcgcctgga cctcctggat gaagcgtcgg
    24601 ccgcaggccc tcgtggctac ccttttcatc ggggccgcgt ttgcttgcct ggcgctgaaa
    24661 ggcggtttcg ccttctacgg aagtttcctt tcggcaaagc aaaccgctga tatcatcgaa
    24721 aaggcacgga gacccggcga cctgatcgtc agctacgaag actacgacca gggaatcccc
    24781 ttctacctga aaacccgcgt tgtcctggtg aactggaagg gggagctgga attcggaagc
    24841 ggaatcggcg atcaatccga atggttcatc agccgcgaaa agttcatgga tctctggaaa
    24901 tccaaccgcc acatgatcgt catcttcagg gaaaatcgat acagggaact gctctcgaaa
    24961 ggagtgggca acatgcacgt tctgggaaga gccaacggtg cggtcgtggt cacgaaccgg
    25021 ccggagggag aaacacaatg agaagggata cgttccttcc cttctcccgg cctagcattg
    25081 acgagttgac gatcgaggac gtctgcgaat ccctccggtc gggatggatc accaccggtc
    25141 caaagtcgat ccggttcgaa aacgccttcc gggaatgcgt cggcactccc tacgcgttgg
    25201 cggtcaattc cgccacggcc ggactgcact tggctcacct ggcactggga atcggacctg
    25261 acgacgaggt catcaccacc ccgatgacct ttgtggcgac cctcaacacg atcgtccttg
    25321 ccggcgcccg ccccgttctg gtcgatatcg acccgggcac cttcaacatg gatcccggcc
    25381 ggctcgaatc ggcggtgacc tcgaagacga aggccatcgt ccccgtccat tttgcaggac
    25441 ttcccgtgga cctggacgcc atcaacgaag tggcgcggcg ccatgggcta gcggtcatcg
    25501 aggactgcgc ccacgctctc ggagcttcct acaaagggag aacgatcggt tccgatccgg
    25561 ggcacatcag cgttttcagc tttcacccta cgaagaacat cacgaccggc gaagggggca
    25621 tggtctgcac ggggcttgag gaggtcgcag aacgcatcgc aatcctccgg cagcatggca
    25681 tgtcgaaggg agcctggagc cggtatgcgg cgagcgggac cccgcattac gatgtcgcgc
    25741 tgccagggct caagtacaac atgctggaca tccaggccgc gatcggactc gggcagctct
    25801 ccaggctcga tgccttcaac gcccgtcggg cttccatcgt ggagcgctac aagagcgctt
    25861 tctcggggct ggaaggcctt gtccttccgg cgaccccctc ctacgagcac gtccacgcct
    25921 ggcacatctt tacaccgctg ctggacttgg accgcttgga cctcacccgg gacgagttca
    25981 tgtcgttgat gaaagaggcc aatatcggaa cggcctatca ttaccaggcc gtgcatcttt
    26041 tcgactttta tcgaaagacg ttcggattca aacccgggga tttccccaat gccgaaagga
    26101 tatccgaaag gatcgtcagc cttcctctct tccctgccat gacggatgag gacgtggagg
    26161 acgtcatcga ggcggtcacc tcgattctta aaagccgcct ccgaagaggg aggggcaagg
    26221 catgacgtcg ccccatcttt ccgtcgtgat tcccgtctac aacgaagagg aaagcctccc
    26281 cctcctcttt gcccgtctga tgccggtcct ggaaaacctg aaccgtcctt acgaggtggt
    26341 gttcatcaac gacgggagcc gtgaccgatc tctcgccctg ctcctggatt gccgcgagcg
    26401 ctaccccggc agagtggtcg tcgttgattt caacggcaat ttcggacagc acatggccat
    26461 catggcggga ttctctcaag cccggggaga aatgatcgtg acgatggatg cggatcttca
    26521 gaatcccccg gaagaaatcc cgaggattct cgcggaaatg gaagccggcc acgatgtcgt
    26581 cggtacagtc cgctccggcc ggcaagaccc gctgttcagg aaattcgcct caaagatcgt
    26641 caaccggatt tccaaccgga tcaccggttt aaagttgaat gattatggct gcatgatgcg
    26701 gggctacaac cgccggatta tcaacttcat caaccagtgc aaggaaacga ccaccttcat
    26761 cccggctttg gcccagaaat ttgccatgaa ccccgtcgag atcccgatcg cccattccga
    26821 acgggagcag gggcaatcca agtacggcct gtatcggctg atccgcctga atttcgacct
    26881 catgacgggc ttttccctca tccctctgca ggctgtgacg atgctgggga tcatcgtttc
    26941 gatcctgagc tttttgttca gcgttttcct ccttcttcgt cgcctcgtcg tcggcccgga
    27001 ggtggaaggc gtcttcaccc tgatggccat cagcttcttc ctcatgggaa tcaccctttt
    27061 ctcggtgggc ctggtcgggg aatacatcgg caggatctac caggaagtcc gtgagcggcc
    27121 ccgtttcgtg atccggaagg tctggaacca tgaaggctga accgcgtgtt cttcttgccg
    27181 cctacagcga ggttggcgtc gtttgcctgg aggaattgct ggcacagaaa gctcaggtcg
    27241 tcggggtctt cacccacgca gacgaccagt tcgaggagat ctggtttcgc tcggtggcac
    27301 gcacggcggc cgaggcaggg atcccggtct tcatgccgga aaacatcaat gccgcggaag
    27361 agatcgaacg gatccggggg cttcggccgg atattctgtt ctctttctac taccggaagc
    27421 tgatttcgaa ggagatcctg gacatcccgc aactgggggc ttacaacatg cacggatccc
    27481 tgctgccccg ctacaggggc cgggcctgcg tcaactgggc cgttctgaaa ggagaaaccc
    27541 agaccggtgc caccctccac gtgatgaccg aaaaggccga cgcgggggac atccttgacc
    27601 aggaagccgt ccccatcgag ttcaccgaca cggcgcagga tgtcttctgg aaagtgcggg
    27661 acgccgctcg aaaggtcctt gccaggacct ggccgctgct tcgcgaagga cgggcccccc
    27721 gaacgccgca ggacgagtca ctggcgacca cgttcggccg gaggaggccc tcggacggac
    27781 tgatcgactg gaacaggagc gcgctcgaac tgtacaacct ggtccgggcc gtgacccatc
    27841 ccttcccggg agccttttcc ttcatcgggg aacgaaagtg gttcatctgg aaagcctggc
    27901 ccctggaaag cggggggaaa gggcaggaag gaaaggtgct gggactggat cccctggtga
    27961 tcggaaccag agagggatct cttcgaattg accgctgcca ggcagaagga gaaccggaaa
    28021 ccgacggagc cgatttcgct tccaggtcgc ttcggcctgg tcaaattttg gggggaaaaa
    28081 ccaattgaag atcctgattc tcggagtgaa cggattcatc ggatcccacc tggcagagcg
    28141 catcctgaac acgaccgact gggaagtccg gggacacgac ctgcgaacga cgaacctgca
    28201 tgcctgcctg aaccatcccc gttttacctt gaggcagaac gacatgcggg aggactggga
    28261 atggatcgaa gcgcaggttt cccagtgcga cgtcatcctt ccgctcgtgg ccgtcgccca
    28321 gcccaagaaa tacgtcgagg accctctttt catcttcgaa ctggatttcg aggaaaacct
    28381 caagattgtc cgcctttgca gccgttacgg gaaacgcctg gtgtttccct caacctccga
    28441 ggtctacggg atgtcccagg atgaggaatt cagggaagac gaaagcaacc ttgtcctggg
    28501 cccgatctgc aagacccgct ggatctacag ctgcagcaag cagatgctcg accgggtcat
    28561 cgccgcctac ggcatgcaga agggactcca atataccctc tttcgcccat tcaactggat
    28621 cgggccccgc ctggacgacc cttctctcgc ggcagaaggg aaatcacgtg tccttaccca
    28681 gttcatctac aacctgctcg aaggtctccc cattcagctc gtcggagggg gaaggcaaaa
    28741 acgctgcttc accgatatcg aggacgggat cgatgcgctg atggccatcc tgaagaacga
    28801 ggggagcgtc gccgacgggc agattttcaa catcggaaac ccgaggaaca atcattccat
    28861 ccgggaactc gcggaaaccc tgcaaagggt cctggcgacc tttccagggt acgaggaaaa
    28921 ggccaggaac gccctcatcc tcgacacgcc gagcgaaacc tattacggcg agggatacga
    28981 agacgttgag taccgggttc cctcgatcga gaaggcccgg cgcctgctgg ggtgggaacc
    29041 gcggatcccg ctggaggaaa ctctccgcaa gaccctttcc gcagcgatcg gacaggaacg
    29101 gcctgcatga gcatcacgct gggactcaaa ctggacgtcg atacgctgag aggctaccga
    29161 gagggggtcc cggctctcct ggacctgttc gaaaaactcg gcatccgcgc ctcgatcttt
    29221 ttctccatgg gtccggacaa tagcgggaaa gccatccgcc gcgtgttccg gaaaggattc
    29281 ctgtccaaaa tgcggcggac ccgggcggtc agcacttacg gactgaagac gatgctttac
    29341 ggaacccttc tcccggcacc cctcatcgtc gattccgacc cttccatcct gcgccgagct
    29401 gccgagcttg gtcacgagtg cgccgtgcac gcctgggatc atgttgaatg gcaggattgc
    29461 ctcgacagga tgagaatcga ggccgttcgt gacgccctca ggcgtgcgtt cgacgccttc
    29521 gagaggattg ccggtcagcc tgcccgatcc tgtgccgccc ctgcctggaa ggtgaccgaa
    29581 agcagccttg aagtgcagga cgaattgcag cttgaatatt gcagcgacac ccggggacag
    29641 ggacctttcc tgcccgtcct tgggaaccgt cggttctcga caccccagat tccctccaca
    29701 ctgccgaccc tggatgaaat cctcggcctt gacgggatga ccccggaaga gtccaacagg
    29761 aggcttcttg atggactccg ggagggaatc aatgttcaca ccttccacgc tgaaatggaa
    29821 ggaatcggtc aaatcgggct tttcgaagaa ttcctcgtgg aatgcaaaaa caggggcgtc
    29881 cggttctgca ccctcgggga aatcgcccgc ttccggtcga gggaggaact ccccctctgt
    29941 ttcctggaaa cgggagaaat ccccggcaga gccggaaagg tcgcattgca gggaaaggag
    30001 tggcgtccgt gaagatcgca atcatcggga cgggatacgt cgggctggtc acgggaagct
    30061 gcctggcaaa ttttggaaac gaagtccttt gcgtggatgt cgacaaggaa aaagtcgaaa
    30121 gtctgaacgc cgggaaagtc ccctttttcg aaccgggcct gggagacctt ctttctccca
    30181 atctctccgc cggccgcctg gcgttcacga ccgacctgga cagcgcggtc aagggagccg
    30241 agatcatttt catcaccgtc ggaacgccgc cgggccccac gggggaggca gacctttcca
    30301 ccgtgtggga agtcgcccgg tcgatcggaa gttccctgga acagggcagt atcgtcgtga
    30361 ccaagtccac cgttccggtc ggaacaacgt tcaaggtccg aacgatgatc gaggaagagc
    30421 tccagaggcg ggggaagacc gtcccgttcg aagtggcgaa caaccccgag tttttgcggg
    30481 aagggtcggc cgtcctggat ttcatgaacc cggaccgggt ggtgatcgga acagaaagcg
    30541 aaaaggcccg tgaaagcctg gccaagctgt attcgttcct cgacgaggaa aagctcctct
    30601 tcatggacat cctttcatcc gaaatgacca aatatgctgc caatgcgctg ctggcgacaa
    30661 aaatctcgtt catgaacgag atcgccggaa tctgccagaa cgtcggtgcc gacgtggaaa
    30721 aggttcggat cgggatcggg tcggacgcac ggatcggttt cgcgttcatc tccccgggtt
    30781 gcggttacgg gggatcctgt ttccctaagg acgtccgggc cctgatccat acggcggcca
    30841 gtttcgggtg caaatcccgg attctggaag cgggggaaaa ggtgaacaac gcccagaaac
    30901 accttctctt caagatgatc gcccatcact ttgaaacgac cgcgggccgg acgatcgcga
    30961 tctgggggct ttccttcaag ccaaacacat ccgacaccag ggaggcctct tccctcgtcc
    31021 tgatcgaaga cctgctggtc gcgggagccc gggtcagggc gcacgacccg gtcgccatcc
    31081 ccgaggcaag aaaggccctg cctcaccatg acaacctgga gttctgcgag aaccacctcg
    31141 aggccgtccg gggtgccgac gccctggttc ttgtcaccga atgggacctt tacaagcagc
    31201 ccgacctcgc ccggctggcg gccttaatgg gctcctgcgt catttttgac ggaagaaacc
    31261 agtacaaccc gcaggaaatg aaacggcacg gctttactta ctactccatc gggagaccgg
    31321 ccgttttggg acgaaggggc tagatccagc ccttacgccg gaaatagcgg agcatgaccg
    31381 cggcaacacc ggccatgact cccaggacgg cgggataccc ccagcgccat tccagttccg
    31441 gcatgaaccg gaagttcatc ccgtagacgc cggcgatgaa ggtcaggggg atgaacaggg
    31501 tggaaatgac ggtgaggacc ttcatgatct cgttcattcg gttgctcagg ctcgaaaggt
    31561 agatgtccaa aaagcctgaa agcgtttccc gcaggctctc gaccgattcg aggacctgaa
    31621 ccgtatggtc gtaaagatcc cggaggtagg gggccagcgc ttcgtctaaa agcgaggaat
    31681 cactgcgctc gaggttaccg atcagttccc ggaggggcca gacgcaccgg cggacataat
    31741 ttagctcgct tcgcagcccg tgcagcgcct gaagcgtttc ccgattcggg gaacttacga
    31801 gctcgtcctg cagcgactcg actcggtcgc taaggctctc caggacgatg aaatacccgt
    31861 caacgacggc atcgaaaaga ctgtaggcaa gggcatccga ccccatcttt ctcagcttgc
    31921 ccttgcctga tcgaaggcgg tcccgaaccg cgtcgaacac atccccggga tcctcctgga
    31981 ccgaaagcac gaatcccttc cccaggatga cgctcacctg ttcggagacg gtccggcccg
    32041 ttctcttttc gagcgtcagc atgttcagga cggcgaaaag gtattcgccg taatcctcta
    32101 gcttcggccg ttgttcgagg gccagcacat cctcaaggac cagggggtgg atgccgaagg
    32161 cctccatgag cctttccagc agggcaggat ccgaatgccc gtccacgtta atccaccgga
    32221 tcccttccgc ttcacgggaa gcgagacact cttccaggct gcagtctgcc ttttcgaaaa
    32281 agcgctcgtt cgtgtaggtg atcaagccca gccgaggggg tttcggtcga gactcctggg
    32341 gtgccttttc ggaaacccgg cggtagaatc cggccctgaa atccctcggc atttccggct
    32401 cctccctaag acgggtccgc ggccacgcgg ttcttcagga tcccgatccc ttcgatttcc
    32461 acttccacta cgtctcccgg aagcatgggg ccaacgccgg ccggggttcc cgtcgcaacg
    32521 acatcccccg gccgcagggt cgcaaagcgg ctgatatggg agatgagcct gccgaccgaa
    32581 aagatcatca tgtccgtcga ggctgcctgg acgacacggc cgttcaggcg ggtctcgatc
    32641 cgcctgcccc gcgtttcccc tgattgcagg atccaggggc ctagggggca gaaggtatcg
    32701 aagcttttgg agcgggtcca ctggccatcc ttgttctgca gatcccttgc cgtgacgtca
    32761 ttcagacagg tcaggccgaa aatgaccgat tgggcctcgt cctccgggat gttccggcag
    32821 gtctttccaa tgaccaccgc cagttcccct tcgtagtcga tccgtcccgc ccagagcggg
    32881 atgcgaaccg tcccttccgg gttcagcacg gatcccgggg ctttcaggaa gatcagcggc
    32941 tcttccggga ggtcgtgtcc cagttctttc gcgtgctcgg tataattcct tccaacacaa
    33001 aaaatgctgc tcggctctac cggagcgaga aggcgaacct cttcgagggg gaaaacctcc
    33061 ccggaaaact cgccgccttc aaaaaacgac cccctgattt cacggaccgt accatcttcg
    33121 aggagcccgg atcgagcccg cccttccttt tcgaatcgaa cccaaaacat tctcatccct
    33181 ccgttttcct cccgttttga tccgtcccta ccgccaattc ggaacgatca gggccgccgc
    33241 cgcgccgtaa ctgaccaaca gcgccaggag caccgaggcg gaaagcatgt ctttcgtccg
    33301 tttcacatct tcgttgaact cggtccccac gtggctgtcg atcaacaact ccagggaagt
    33361 gttgatcagc tcgatcacca tcggtaccag gctgagcagg aagacttgaa aggccagcca
    33421 aaattctcct ttccccgcgt aaaccgccaa tgcggtcaga atcacggcac acgccgtttc
    33481 ccttgcgatc gcggtttcca tgaaaaaggc cgtcttcagc ccattcaggg aattcatgaa
    33541 ctttctcgcc agagagtcgt ttttccagca gcacttcgtg ttcggattac tccccatcca
    33601 gggttccctc ccctgccatc gcggaggccc gaacgaaatc ccgggctttt cctgcaagcc
    33661 gtaccacctg ctggattcga ggcaacttgt tttcaatcct ttcgatgacc cgcctttcta
    33721 aaattctccg gaaggtgggc tgaaaattca agtcaaagac ccaggacaac tgcacgagga
    33781 gaaaatcggg aaccgatcgg acctggctgt aggatgccct gcccgtttca cccagggtgt
    33841 ccaggagttc cccgctgatt ccccccgtat ccgggatcct tgggaggagt tcatggatcg
    33901 ttccccggtc caaataatcc tggactagct cgaagacatc cagcttgtcg gcatcccgga
    33961 ccagcctgac catggtcagg ctcgaaggtc ggatcccgga agggacctcc ttcttgttgt
    34021 gaaagcggat cccatcaaga atggcgagac tcgcttcatc ctcgagcgga tcgaggatcc
    34081 ccgcccgctt cacggtttcc cagcccaatt ccccgtgatc gacggaggcg gcatcgtaga
    34141 aggtccggaa ctctcgaaac tgggggaaac ggccgacatc gtggagtaac cccagcgttt
    34201 ccgcgacatc gaccgagcgg gcgtccagcc ccatctcccg ggccaggaaa cccgcttcac
    34261 cggcgacacg gaaactatga cgcaacttga ggtcgagcag aggttcgagg ggattatcgc
    34321 ctttctggaa actccgcgtg taggaagcga accactcccg gatttgctgc atcgaattca
    34381 ccgtccagac attcctttcg ttcattctca tcggcctttt ccttgcggcg cctgttcctg
    34441 gctgatatag taatcgaacg gctttcaggg aaggagcgat ttccgttgga ttcttccgaa
    34501 cgttacaaga agacgtttcc gatttcctgg gatcagctgc accgggactg caaggccctg
    34561 gcctggcggc ttcacgagaa ggggagatgg aaccggatcc tcgcgatcgc ccgcggcggc
    34621 ctggtcccgg cggcgatcat cgctcgggaa ctggacatcc atttcatcga cacgctctgc
    34681 gtttcaagct ataccctccg aacccaggga gaaccggcaa tcctcaagct ggccacttcg
    34741 ttcgggaacg acccccaggg atccctgatc attgacgacc tggtggacac cgggagaacc
    34801 gcgcagctgg tccggcggat gttcccggaa gcctgttttg ccaccgtcta cgccaaaccc
    34861 gagggaaggc ctctggtgga tctcttcatc acagaagtca gccaggacac ctggatcctc
    34921 ttcccctggg attcggaatt ttcgttttcc cagccgatca tcggaaggac ctagtcccgt
    34981 tcttcccggt ggaaaggctg ccctagcgag gccggagcgc ccaggaggat tccctttccc
    35041 ttcctgaccg aaatccccac cagcacgacg atcgtaagga tgtagggaag catcagaaga
    35101 agcggagccg gtacggtggt ccctgccgcc tggattcgaa gttgcagggc cccgactcct
    35161 ccgaagaggt aggaaccgaa agccgccctc agggggttcc agatcccgaa aatgacaagg
    35221 gcgacggcga tccagcctct tccggcagtc atcatctcca cccacatctg cgtgtacacc
    35281 accgagagat aggctccccc caaagccgcg agacctccac cgaccatcgt ataggcgtac
    35341 ctcagccggg cgacgctcaa gcccgccgca tccacagccc gcgggttttc gccgacagcc
    35401 cgaagggtga gtccccagcg ggtcgaggag atgaaccagc aggccaggcc cacgaggacg
    35461 taagaaagat agaccagcgg atcatggttg aagaggacgg gtcccaggag ggggatcttc
    35521 gccagcacgg ggaccggcag aatcccgagc ccccggatcg tttcccccac gtaggccttc
    35581 ccaagaaggg cgctcatccc ggttccaaac atggtgatcg ccaggccgct caccacctgg
    35641 ttggctccca tggtcacgca gagaaacgcg tgcagggcgg aaacggccat ccccacgcaa
    35701 accgccgccg cgaggccaac ccagggattg cccgtgacca tcgtcgcgga gaaacccgaa
    35761 aaagccccta gcagcatcag cccctcgagg cctaggttga gcaccccggc cttttcggtc
    35821 aggatctcac cgagggtcgc atagagaatc ggcgtcccgc tccgaatggc ggcagaaagg
    35881 attgggatca ggatttccat tcccctcact cccctctttc gatgaaaacg atccggtagt
    35941 tccggaagaa ctctgccccc agcacgcaga aaaggatcaa cccctgcagg acctgaacgc
    36001 tcgccagagg aagccgcatg acaacctgga gcgtatcccc gccgaccaga agcacaccga
    36061 ggaggaagga aaccaggatc gtcgccatcg gattcaagcg ggcaagccag gccacgatga
    36121 tcgcggtgaa gccgtacccc cccgaaaagc caggctggag acgtccctgg agccccgcaa
    36181 cttccgtcat ccctgcgatt cccgcaacgg ccccggaaag gaacatcgcc aggaccacgt
    36241 tcttcaagtc gttgatcccg gcataccggg ccgcgttcgg gttgttcccg atgacccgca
    36301 gttcgaatcc ccactttgtc cagcggtaga gcacccgaaa aaggatcgcc aggccgaccg
    36361 ccagaaaaat cccgacatgg accctcgttc ctcccaggac aggcaggcgg gcggcttcag
    36421 ggaagggggt tgtcatggga aacccgagac tcgacgggtc tcgccatgga ccgtagatga
    36481 agtaatcggc gaggagaatg gcgatgtagt tcatcatcag ggtggtaatg gtctcgttca
    36541 cattccaacg agccttgaga aaacccgcga aacctgccca aagccctccg gcaaggcagg
    36601 ccatcaggaa catgagagag aacatcacga gggaattgtc gacgaaagcg taccggacga
    36661 ccgcgacggc cgccagggct cccatgacga tctggccctc ggccccgatg ttccacacga
    36721 ccatcgtaaa cgcgagcccc accgcaagcc ctgccaacgt tagaggaatc gccttgacaa
    36781 tggtctcgct gatcccccag gcatccccca gcgaaccctg gatcattgct ttataggcgg
    36841 caagcggcga aacccccaaa agggacagga ggacggcccc ggccgccaga gcaaccccca
    36901 ccgctccggc ggggatggac cagttcatcc aggaaggaac gtccaaccgt ctttcgatcc
    36961 gcaaacgcgg tttcatctgc ccttctctcc ttcaatcgcg gtttccctcc ggatccccgc
    37021 catcatcagc cccagttcct cctcggaagc gctccgggga tcctcgacca accccatgat
    37081 ctcccccttg tgaatgacgg caagcctgtc gctcagggcc aagacctctt ccaggtcttc
    37141 cgaaaccaga aggatggccg ctccccgttc ccgctgacgg agaagctggt cccgcacgaa
    37201 ctctgtcgcg ccgacatcca gtccccatgt cggctgcatc gccacaatca aatccggggt
    37261 tcctgaaagt tccctgccaa gcatgaggcg ctgctggttc cccccggaga ggtttcgaac
    37321 cgggatcccc aggccggggg ccgaaacacc gaacctttcc acaatccgtc cggtttcccc
    37381 ggcgatgctc tcccagctca ggaacgggcc cttggcatac ggtttcctcc agaagttctt
    37441 cagggcgaaa ttttccctca ggttcatggc tccaacaagc cctgtccctt tccggtccgc
    37501 tggaatgtaa ctcaccccgg cccgaaggaa atccatgggc ttgcgccccg tcatgtcctt
    37561 tccccgcagc aggacccttc cgaactgaat cggcctcaaa cccaccagcg cttcacagag
    37621 ttccttctgc ccgttcccgg cgatccctgc aatgccaagg atttctcctt gcctgatcga
    37681 gaggctcagg ttccggatcg ccggaagacc acggtcattc agcgcgcaga tcccttccgc
    37741 ctccaggacc gtatccccgg gattcagaac ggccttgtcc actgcaaaaa cgatctttct
    37801 tcccaccatt ttctcggcca gcatttcccg ggttgccatc gggccgggca gcgtctcgac
    37861 gacctccccg ctccgcagga tcgtgattcg gtcggagatc tccatgacct catccagctt
    37921 gtgggagatg aacaccaccc cgtgtccttc ggccgtcatc tggcggatcg tatcgaaaag
    37981 ccggcgaact tcctggggag tcagaaccga tgtgggttca tccaggacga gcacctcggc
    38041 attccggtac agcatcttca aaatttccac ccgctgctgc tctccaacgg aaagctgcca
    38101 gacctgggct tccggctcaa catcgaggcc gtaccggtcg gagagttcgc cgattttccg
    38161 gatcacttcc cttttgggca gcacggcctt caggcccgga agccccatca ccaggttttc
    38221 ccagaccgtg aggacgggaa caagcatgaa atgctggtga accatcccga tccctgccgc
    38281 gatggcttcc cgcggcgagg agaagcggca ttcagcccct ttaatgcgga tgctgcccgc
    38341 atcgggctgg tagagcccgt ttaggatatt catcagggtg cttttccccg ctccgttctc
    38401 acccaaaagc gtatgaacct ctcccgggaa aacgtcaaaa ttgatccccc ggttcgcttt
    38461 gactgacgcg aagcttttcg tgattcccag catctggaca agcggctgtt gttttcctga
    38521 ttccatgagg taggccctcc gggaagaaga atacctgaga ccttccaagt ttgcccgaat
    38581 gagcggaatt gtcaacgctc agcaaaaagg ggggcccgaa cgagcccccc tggattcccg
    38641 cctgcgggcc tacttcggga tcgtcccctc gaccccctga acaaaccagt taaaggagag
    38701 catctccggg tcagacatcc gtttgcccgc ttcaaccctg atttttcctg attgatcttt
    38761 gatgggcccg gtaaagacat cccactttcc cgaaacgatc ttcccttttt cttccgcgac
    38821 cagtttccgg acgctctccg gaaccgcctt cccgaaaggt gccaggccga ccatgttttc
    38881 cttcagcccc caccagatcg attcggattt ccaggtcccc ttgatcacgt tttccgcagc
    38941 gtacttgtaa agcatgcccc agttccagat cggggcagtc aggtgccggg tcggggcgta
    39001 gcggctcatg tcgttgttgt acccgacaac ccagaccttg gcttcctctg cagcctgggg
    39061 ggcggcgccg gagtcggcat gcattgcgat aacgtccgcg ccggagtcga tcagggcctt
    39121 ggtcgcttcc ttctccttcc cggggtcaaa ccaggagaac agccagacca ccttgacctt
    39181 cgccttcggg ttcaccttcc ggacacccag cgtgaacgcg ttgatgccgc gaatgacctc
    39241 agggatcggg ttcgccgcaa cgtagccgat gatgttgctc ttcgtcatct ttccagccac
    39301 aagcccggaa aggtaccggg gctggtagat ccggccgaaa taggtcccga cattcggagc
    39361 cgtcttgtag ccggagcaat gcatgaaaac gacgtcgggg tgctttttgg ccacttcctg
    39421 gacatagtcc atgtaaccaa aggaagtggc gaaaatcagc ttcgatccgt ttcgcacgaa
    39481 ggtttccatg acccggactg catccggacc ctccggaacc gattccacga tgctcgaggt
    39541 cactcccgga agcgccttct ccatgtcctt gcggcccttg tcatgcatgt aggtccagcc
    39601 gccgtctccc acgggtccca cgtaaacgaa cccgggtttg aggtccttcg ctggaacagg
    39661 agcaaaggcc cacgctgttg tcgctgcaag caggaaagcc atgacgaatg caaatcccaa
    39721 accccttctc atccttgttc cgcctcccga atatgacgtt tgaaccgcaa cccgtcttct
    39781 ttctattgat accatggcat acatttaacc gcaagacccc aaaaatatca gcccttcttc
    39841 cctggctcct cgcgttggaa gggctcgtca gggtttttcc ctggtgatcc ggatctcaaa
    39901 aaggcggctc cacggatagc tcacctcaaa atcggcgaag ccgaaatccg ccaggggccc
    39961 ctgggagaaa gcctgcagtt tctcccggat ttcgccctcc agccttccag caaccgcttg
    40021 acccgcgaat ggccctttcg ggaccacggc cgggcgaacc accgcctggt acccccagtc
    40081 atccaggtac cgcgtaaaga ggagccattt gcggtcctcc tcggtcatcc aggcggacag
    40141 gactcccgtt gccagggcta ccccgatgct gttgtccgag gtgttccccc ccgagtacgc
    40201 tgcaagccgg gtcagaagcc cctctttcct cagggcttcc atcaaccccc ggtccgcccc
    40261 gttgtcaaaa gcgatatcgg caacagccac ccgttttccc gtatccaaaa tcctcccgat
    40321 ttccccggcg aacgcctcgt cggattcgtc cggcgtcgcg ttgttcgcgg gggacgacgc
    40381 ttccagcgtc cgctccccct gcggcaggtt caccacgagg accatatcgg cctcctgggg
    40441 ggtagcgact ctttccactc cgaggacatc cgcctggatc tcgaaagatt cttcaggagt
    40501 aaagccggaa tagttcggtt gcagcttctc gccgaacgag cgcgggaaca gcaggcagac
    40561 tttctgccgg ggcttatcgt gaaggaccat cctggcaaac agcaggaggc ccatctggtc
    40621 cactcccggg acaacggcgg ccctgtcccg ggaagcctcc ccgatcaccg atgccatcga
    40681 gcgaaattcg ggttcgagca gctgcgggca aacgttatcg tctaggccga tcccaacaaa
    40741 atcgaccccg gcagcaccgt gaagggcaag gagccggttg gcggctcgaa ggctccggtt
    40801 ccgcctttcg gcccactctc ggagaaccgg aaccggaatc gttttcagga gccgctccgc
    40861 cgctgccagt tcttctttgg aaagctctcc cctggatgcg gccgtctgga gcctggccaa
    40921 tcggaaaagt tgcggggccc attttgcgtt gtagtctgca tccagaaccc cctggctttc
    40981 cctgggcatt cgaagcagcg tcccaaaaag ggcaattttg ggcttctgga ggctgtcgca
    41041 aaacttcttg agggcctcga agcgccatcc aacctcttcc tcatccagat catggatcct
    41101 cgaggagacc agccccccga aaagaagggc atccgccgcg atcacgaatg ccccttgact
    41161 ggaattggat gccagccacc ggaaaagctc ttcatagtcg gaaactccag cctgccccct
    41221 gaaaaaccct gccggaggga gcaaaagctc gactttcaag gcccttgcgg cttcgatgac
    41281 ccgatccagg gaatacggcc ggtcatcgag gggcacgtag acgacgagac ccgcaaacac
    41341 tggacgggcg acaaaaggca ggagcaaaac cattgtcagc agtctcagca gtggtttctt
    41401 gaacctgctc atcgccggtc cacactccct gaaagagaaa aagccccatc gtacgatggg
    41461 gctggtccta tgctggctcc ccgacgagga cttgaacctc gaacctagtg gttaacagcc
    41521 acccgctctg ccgattgagc tatcggggaa ttccgtttcg aatgccgagc gagaaaacgc
    41581 ttcagcggtt ggaaatatag cataacctct ttcggctggc aagaggattc tggcaatctt
    41641 cagcgcggca tcccctgtag gcagagaggg ggttccagac cgcaacgctc caggagagtg
    41701 cggtcctgga aaatcctggc cgtttcccca tcggcttcga cccgcccccc ggaaaggacg
    41761 atcgtccggg gacagacttc gaggaccaaa tccaggtcgt gagttgcaac cagacgggtg
    41821 tgcgtgaatg acttcaggag tccgatcaga aggcggcgcc ctctcgggtc aaggctagcg
    41881 ctcggctcat ccatgatcag gacgtccggc atcatggaaa gcacggtggc gatggcaacc
    41941 gaacgtttct cccctcccga aagacggtgc gccggccgtt ccgccaggtg acgtgcgcca
    42001 acttgctcca gggcggttct cacccgttcc tccacttctt ccgcagtaag cccctggttc
    42061 aatgggccaa atgcaacgtc atcaaaaacg gtcggcataa aaagctggtc gtcagggtcc
    42121 tggaacagga aaccaattcg cctccggatt tccccaaccg tctgttttga aaggaccgtt
    42181 ccgccaaggt tgacggagcc cctgcatgga ttgatcaatc cagccagagc cagcagcgtc
    42241 gtcgattttc cggccccgtt ggcccccaca agtccgaccg cctccccgtg ctccaggcgg
    42301 aaggaaaggc cgcgcaacgc ttctgtccca tccggatagg caacccagag gtcccggact
    42361 tccaggaaat ggtggctcat cgtcctagaa cccacccgaa cagccggcct ggaacgtcca
    42421 cgaaccggac cgtcaggaaa tagagggtcc agaacccaac gaatatcccg tcccggctcc
    42481 aagcaaaccg aagaggcttc atcaagcgta cttccccgtc aaaccccctg acgagcatgg
    42541 agcggtagat tcgagtcgcc cggtccaggc ttctcagcag gagaaacccg atcatcgaag
    42601 ccgcgctcga aacccggatt ttcggacggt tgcccctggc cctcagggca tgggcacgga
    42661 ccatccggac ggcctcctcc tgcaggaggg acagatacct gtaaaggaac tgaagctgcg
    42721 tgaccaaaac tcgaggaacc cccattttct caagtccgag gcagagtctg tcaaatggcg
    42781 tcgtcatcgc caggacaacg gccgccgcta ccgccagaag cgatctgaac gtgatcgaaa
    42841 caaaggagat ccaccctcct gatatcgaaa gcccccatac cttgaaaagg atctcccggt
    42901 cgaaaagggg attgaacaat cccacaaaaa ggacaaaggg aagggcaacg aggactcttc
    42961 gtagcagttt cgtcacaggg acgcaggaaa ccgccgtgag ggaaacggga tacaggacaa
    43021 gcggcgtcag gcgagaaatc tcgtacttcc cgaaggaagt cacgacaagg aggaaagcaa
    43081 acgtgacgag ccactttgcc ctgggatcca ccccgccccg gctaaagaaa ccggggtttt
    43141 cgtccaggct gcagccgctt gttggcaaac gcttccccta ctttcgccgg gaaacggcac
    43201 cccgaagcag gatcccaagc cctcccgcaa gtgtcagcgt caaggctcct ccaacaaggc
    43261 cggaaaccgt tgttgcgggg tccaccgcgg gccatttccc ttcattttcc ccggccgcag
    43321 ccgcttccgg ctgagaatct ttcccggccg gtgaggggcg gaaaccataa tccggcagga
    43381 acgccaattt ttcctgcagg gccgcaagcg ccgtgtgaat cctttccgcc ggcgcctcaa
    43441 gctcctccgt accggcggtt ttcgccatcg accattccag tccgtccgga taggtcgagg
    43501 caaaccagga aagggcacct ccggtgaaaa tcgcggcaag ggcaagtccg gccaggactt
    43561 tcctgaggga aacgcctggt agcggcgtgt tccgcgcggc ggattcaagc acttcaggcc
    43621 gggccttcca gacgaaggtc acgaccgctc ccgtgacgat cccttccaca atcccgatcg
    43681 ccaggtggat cggctgcatc aggagcaaga aagtcctgaa gggcaattcg gaaattcccg
    43741 aggccacggt ttccagcacc acggaaaagg cgccgagctg aagccctgca acagctgaaa
    43801 ggatcgcgcc aagaaggatt tttttacggg aaggggagtc cccgacaatt ttcctgtaga
    43861 tgaaaggata agcgacaaaa cacgggaaga aagccaggtt gaagatgttg caccccaagg
    43921 ccaggatgcc tccgtcggca aagaagagcg cctggacaac cagaaccgaa gccagtacca
    43981 ggaatgccgc atagggcccc agcaggatcg ccaggatcat cccccctccc aggtggccgc
    44041 tcgaccctgt tgcaggaatg ctgaagttga tcatctgcgc ggcgaaaatg aaagccccta
    44101 aaactcccat cagggggatc ctggattcat ccggcgattc cttcagtttt ctggccgaat
    44161 aggccgtcag gcctgccgtg accgcgagca tcgtcccgcc taccgccgga gaaagaagcg
    44221 catctgccat gtgcatgaat accaccccta tcttcgtgtt acttttatta gggtaaataa
    44281 cactacccgg gcccgattgt caacaaaaaa aacgggcagg ggagcacccc tgcccggaag
    44341 aacacgggct attcagccct ttttctgctc ttttgcccaa ctgtccttga gaggcacgat
    44401 ccggttgaac accggacgtc caggcctgga gtcgggatcg acgcagaaat agccgtggcg
    44461 caggaactgg aagcgctgcc ccgcctccac cctggaaagc cccggttcga ccctgcaccc
    44521 ggaaaggaca accagtgaat ccgggttcag gtagtctcgg tattccttcc cctcctcgac
    44581 ttcatccagg ttcttcaggg tgaaaagatg gtcgtaaagc cgaacctccg cctcaacagc
    44641 atgagatgcc gaaacccaat gcagggtccc cttcaccttt ctgccgtccg gggcatcccc
    44701 cccacgggtc gccggatcgt actcgcaacg gagttcgacg attctgccct cttcgtccct
    44761 gaccacggac ttgcaagtga tgaggtaggc gtgcttgagc cgtacttctt ttcccgggga
    44821 aaggcggaag tagcctttcg gcggattctc catgaagtcg tcctgttcga tgaagagaac
    44881 ccgggaaaac ggaattttcc tggtcgttgg tgtttccgat tccgggttgt ccagggcctc
    44941 gatctcttcg acccgatcct cgggataatt ctcgatcacg actcgaagcg gcttcagcac
    45001 ggccatgacc cggttcgccg tcttgttcag ctcttcacga atacagaact gcaaaaactc
    45061 gatgtcaacc atgctgttcg tcttcgcgac accgatttcc tcgcagaacc gccggatcga
    45121 ggaaggggtg taccccctac gccgaaggcc cgagatcgtt ggcatcctgg gatcgtccca
    45181 tcccgaaacg catttcgtcg tgaccaattc aagcagcttt cgcttgctca tgacggtgta
    45241 ggtgaggttc agccttgcaa attcgtactg gcggggcctg tggggaaccg aaacgttatc
    45301 gaggaaccag tcgtagagag gccggtggtc ttggaactcg agggtacaga tcgaatgggt
    45361 gacgccttcg atggcatcct cgtagccgtg ggcgaaatcg tacatcgggt agatgcacca
    45421 cctgtccccg gtgttatgat gcgactcctt caggatcctg tacatcacgg ggtcccgcat
    45481 gttgacattg ggatgcgaca tgtcgatttt cgcccgcagg acgcactccc cttcctcgaa
    45541 ttccccgttt ttcatctttt cgaacagttc gaggttttcc tcgacggtcc tgttgcgcca
    45601 gggcgactcc gttccgggtt ccgtcagtgt cccgcgggtt tttcgaattt cctccgggga
    45661 ttgatggtcc acgtaggcct ttccggcctt gatcagttca atcgcccaga ggtaaaactg
    45721 gtcgaaatag tccgaagcga agcagaggtt gctccacctg aaaccgagcc actcgacgtc
    45781 tttcttgatc gactccacgt attcgatttc ttcctttgcc gggttcgtgt cgtcaaaccg
    45841 gaggttgcaa tcgccgccaa atttttccgc caggccgaag ttcagacaga tcgacttggc
    45901 atgcccgata tggagatacc cattgggttc cggcgggaag cgggtgacga cttttttgat
    45961 tcgccccgac tccagatctt cccggatgat ttgttcgatg aaattgccac ttttcggctc
    46021 tgcctgttcc atcttgctcc tccaccctca tgatcaaagt tacggatctt cccgaatgtt
    46081 ttatcacaag ttgcttcttg tcccaatcgg agaatccgcg caaacacggt ttcgaccggc
    46141 ggctttagcc tggtagagcc gttcgtcggc cacttccagc attttttcca ccgtcagttc
    46201 cgaaagggtg gagaattcct ccgtgtcgac aagacccgcg ctgaatccca tggagagctt
    46261 tcgcccggga gcggcccgaa tcgaaagccg gacttcctcc agtagcccga gcagaaggat
    46321 ctccgcttcc cttttcgagt aatcggaaaa gaccagcaga aactcatctc ccccatacct
    46381 cgcagagacc tgcccgggga cggaactttt tcgaacaagt gccgcgaatt tttccaggac
    46441 atcatccccc gcccggtgcc ccagggtgtc gttgatccgt ttgaaatgat cgaggtcgag
    46501 cagagcaagg gaaaagatcg ttccatcgga tcgctttccc ttgaccaact cttccaggag
    46561 accatacgcc gcccgtatgt tcatcaaccc ggtcaacggg tctacaacgg ccgcttcccg
    46621 aagctgctcc tcgacttttc ggcgaaggct cgcgtccctg aaaacgaaaa gctttcccat
    46681 cacggcaggt ctgcccccgg ccctcctgtc cctgagcgtt gaaaccttga cttcgtaggc
    46741 cagcttcccc ccatcgggca aaacccgttc gacctccgcc aggccctccc cgtctccgtc
    46801 gaaaaagcct gaaatacccc accagtccct aaaggcctct tccatcgtca tcccgtagat
    46861 ctcacccctg tcccggccca gaatggaggc cattgccggg ttgaggtccg cgatccctcc
    46921 ccgaggatcg accacgagca ctccatccgg catcgtttcc accaggagac tgcgggcgac
    46981 cggaacgaga tccaggattc ccaggcgggt gatgccgacc gtgagcatcc cggatgagaa
    47041 aagaaaggca agcggcatcc agtctggagt ttgccctagt gtcccccata ggaaggaaga
    47101 agcgttgaca accaggggag ccagggatcc cgcgagaaga acgaaaaact gcgacattgc
    47161 atgacgtgaa acctgcaagg cccgtttgac gatgagaagc gctcctgcaa aaagcagcac
    47221 cgatgaatag acgacatgca cccagaacca ggggccgggt tcaaagaaca gccggccggt
    47281 ttccgggttc agcagaattt cggaccagag gccatgaaac cgttcgtttg tgatggcaag
    47341 gaaaaccgtg atccccggca gggcaaacag aagggaactc cagtaccgtg tcgggaaatt
    47401 gcctgccaaa gcgtagcgga gggcaaaaag aaaaaagaag acaggacagc tggcgattgc
    47461 cggggtggca acctgcctcc agaacagctg cagccccgcc gtcgcggccg cgctttcgag
    47521 aacttgcgca aagagccaat gagctaccgc ccacaaaagg agcagcaggt agacccccgc
    47581 tcccccgatc cgttttcggc ggacgaaact cagaaatccg gatgcaagaa ggcctgaaat
    47641 gagaaagaga gcgctgcagg cctggaaatg ataatggtcg ggagaaaaga gtccgttcat
    47701 gaaagtattc taaacgatcc agccctgatt cgggaagccg cccttcccag ccgtttcggt
    47761 tcgttcgctc tccctttcaa aacgagcgcg ttccacctct tcaagaaagg actttttctc
    47821 ccccctcctg cgacttttga ctcttctttc gactgcctgg gcttctgccc ggttaaacca
    47881 gcatgaagtg gcatattcca gctccaccgg aaggtgagcc gcggtgtacc tcgccccttt
    47941 gccttcccgg tgctccctga cccgccgctg gaggtccgtc gtgatcccgc aatacaagga
    48001 accgtctccg cagcgtagaa ggtagacgca ataccgaagg ccgttcattc gctccgatcg
    48061 ctggcaaact gacactccaa cgttttcagg agattgtagt acaaccggtt gacgggggtg
    48121 ggaaccccgt gctgttctcc cagacggcaa accttgccgg caaaggtttc aatttccatt
    48181 ttccggcccg cctcgaggtc ctggagcatc gagtttttcc cttccggatt gaattgcctc
    48241 aacatcaaga ggctttcctc caggtcctcc tctgttaggg aaaccccgga atatggagcc
    48301 aatgccatga cttcccgcat cgccgcaaaa gccgcttcga tgctttgcgg gacttcctgg
    48361 aatacccggt agggagcctt caggattgtt cctgtctgat tgattccaac attgaccatg
    48421 aacttccacc acatcttttt ccggatttct cctgtggctt cgtaacgaac cccggcttcc
    48481 tccaggagac cggccaaccg cttcatgcgg ggcgagaatg tcccatccgc ctcgccgtac
    48541 acgatctgcc ccgggctaga atagacgagc cggtccccct cccttgtcga atccattctt
    48601 gcgacaaacg agggaaggac cttgtcagcc cccagcctgg ccgcgatgac ctcttcactg
    48661 tcgatgccgt tcagcaggga aaggacaacc gtctgggggc ctaccgcctc ttccaggagc
    48721 ggaaggacat cctgaagctg gtacgccttg acagaaacaa aaacgaagtc gcaaggcacg
    48781 gagatctccg agggcatcag gcacggaaac gcgtacagcc ttccgtttac aaacctttcc
    48841 ctgtctgcgt atttctcctt ccggatcctg tcgcagagga aatgaaaagg gtaccccctg
    48901 tcggcaaact gggaggcaaa ggtcatcccc acggctccca ttccaaggaa cagcacgttc
    48961 tccattctca ccactctccc ctgggttcat acaggaacaa cgattccgat ttcagtatta
    49021 ttataaccgg gagatttttt aatggttggt aggatagaat gttgcctgaa agacgcaaaa
    49081 ccggaggcaa aacggggagg caggtccatg cagaattctc tggaagacta cacgaggatc
    49141 ttcgacgcac ttcttcaaaa ctcgcaagat gccgttgctc tttgcgacac ggatcaggtc
    49201 atcctgaaaa tcaatcgggc cttcacggaa cttttcggac ataccgagga ggaggcagtc
    49261 ggaaagagac tggacctgct ggtcgccacc acccccgaga tgatggccag ttccgacatg
    49321 caggtggatc ggatccggat ctgggaaaaa gtgttcactc aggccgttcg tcgtacccga
    49381 aaagacggct caacggttcc tgtttcgatc gaggggaacg tcttccgcct tgccaacggg
    49441 gagaggctta tcctctggaa ttaccgggaa atccccgcag aagaaggtcc cggcaattag
    49501 aatccatttg ccgggacctt cttcatttgc gcatcagacg gatcagaacc aggaggacga
    49561 cggctcccag cgtcgcgatc agaaggttcc cccagaaacc gcttccgaag gacaatccca
    49621 cattcctcgc gataaagccg ccgatcaagg ccccgacgac cccgacacca atgttgccca
    49681 ccagcccgaa gccccctccc ttcacgaggt gaccggccag ccagcctgct agaagcccca
    49741 cgaaaacaaa ccccaaaaag ctcaccgggg cctcctacga gcgggccagt ttttccctga
    49801 cccttttcag catatcggtc gtcatcgaag cgagatcgta cttcggggag aacccccatt
    49861 cgatccttgc cgccgaacag tccagcgagt ccggccagga ctcggcgatc ctttgccgga
    49921 ccgggtccac ctcgtacgag atcgaaaaac ccgggatgtg cttctgaatt tcaagcgcaa
    49981 tctgttccgg ctcgatgctc atcgcggaga tgttgaaggc attgcggtga accagcctgg
    50041 aaggatccgc ttccatgagc tgcacaacgg cttccagggc atccggcatg tacatcatgt
    50101 ccatgaacgt ccctttcgca atgtagctgg tgtaggaccc cttcgacacc gcatcgtagt
    50161 aaatgtgaac ggcgtaatcg gtcgttcccc ccccgggaag ggccacatag gaaatgaggc
    50221 ctgggaaacg cagccccctt gtatccaggc cgtacttcag gtggtaataa tcgcaaagca
    50281 gttccccggt gaccttcgcg attccgtaga tcgtcgccgg gcgttgcagc gtatcctggg
    50341 gggtcatctt ggggggagtg ttgggcccga aagcggcaat ggaactgggg aaaaacaccg
    50401 cggccccggt cgatcgggca cattcgagtg ccgtgaaggt cccgttcgcg ttgacgtccc
    50461 aggctagctg cgggttggcc tccccggtgg cagacaaaat ccctgccagg tgcatcaccg
    50521 tatcggcttt gaaccgtgag agcaggtcca aaaacgcttt cccgtctcgg acatccagca
    50581 gctcaaacgg gccggaatcc cgcatttcct gggaagcggc cctgcagtct gtcgccagaa
    50641 cgtttgcgtc cccataaagc cgtctcagat aggggaccag ctcaacccct atctgtccgt
    50701 ttgcccccgt cacaaggatt cgtttcatcc ttttcgccat ctcctctctg aacccgggca
    50761 ggcttgtccg ttcggttccc gcccgcatga ctcgcgtcag gcttagtatc ggtctgcccg
    50821 ggaagctctg tcaaagtgct atattttaag aacgcttcct atattccccc ccgctctttc
    50881 tataaaataa ccgagacagg aaatgaattc cgggaaggtg ttgaacttgg atcaccagat
    50941 ggaactggaa gaactggcct tgcggatcaa gggggactca agcgcgccct attacattgc
    51001 gaacgtcctg cgggaagcga tttaccgggg catcctcgaa gaaggcaagc ctctccacca
    51061 ggcgaccctc gcgcaaaggc tgaacgtcag ccccattccc ctgagggaag cgctgcgact
    51121 tcttgaaacg gaaggactcg tgacgtttcg aggatgcagg ggagcggtcg tcacggctct
    51181 ctccctggaa gaggcccgag aactctacga aatgatcacc gcccttgaaa ccagcctgat
    51241 gcgcatcgcg ttccccaaaa tcacgcggag aatcctcgag gaggccgccg ccattctcga
    51301 ggagatggag tcggaagaag actgcatccg gtggagggac ctcaaccaga tgttccacaa
    51361 tcttttctac gaacccgcgg accgtcctct caccctcgat atgctggctc gcctcaggca
    51421 gaaaacggac cgcacgatcc gcgttcacct cgcatcgatg agaatcgact cccagaaaca
    51481 gcaccgcgag atcctcctgg ctgcggaatc aaaagacctg gagggcactc tcgctgcctt
    51541 atccaaccat ctcacctaca cttccaacga cctccagtcc tgcatgagag ccgaggcatc
    51601 cacccggggc aaaccccctc aagcttaaaa gacccgggac aacccatgcg cgacgagggc
    51661 gacgacggtg gataggaatg ttccccatgc aagatccacg accgtcacta gcaccggcca
    51721 tccctgcagg gtagccaggt tcgtcaggtc ataggtagca tagcaaacaa gaccgaacaa
    51781 agcccctctc attgcgacct ggagcggcgt cccaggtcga ccggtgacga aaacaaccat
    51841 ccctgcgacg aagagaaggt aaaagatccc tgccgcgaac caattcggct tcggggccag
    51901 gatggatccg atctgagact tgtagaacct cggggcgacc aaccccaacc acagggcatc
    51961 gatcccgata aaggggagca gggtcaaaag gtaaacttta aaaaacggca tggttccgac
    52021 tcgctcaata cttctccgac aggacctcga aatatccctg cggatgagcg caggcagggc
    52081 acgtttcggg tgctgaatcg cctttatgga tatagccgca attgatgcat ttccattcga
    52141 ccggctcact ctgtttaaaa acggtccccg agcaaagatt ggagtaaagc ttgcggtacc
    52201 ttgcctcgtg ccgcttttcc acttcggcga tcaaccgaaa gacagcggca atttcggcaa
    52261 agccctcttc ctcggccacc tgggcaaagg ccggatagag gtcgtgccac tcttcgtgct
    52321 ccccctctgc ggccgccagg aggtttcttt tcgtgtcgcc caaagcgacc gggtaagccg
    52381 cttccacagg aaccgcttct ccgtcgaggt cttttaccag gaatttaaaa aaacgtttcg
    52441 cgtgctcctt ttcattttct gcggtttccg taaagatatt tgaaatctgg atcaggcctt
    52501 cctttttcgc tatggatgag tagaaggaat agcggtttct cgcctgcgat tcccccgcaa
    52561 acgacttcat cagattcttt gccgttttcg tccctttcag gcttttcatt ttcatcacgc
    52621 tccctgggat gagggttctc taccggaggc cggaggtgcc ttcgcctctg ctcttcatct
    52681 gattatacct tcaatctggc agacagaatg gcaactagaa accctgacgg tttgttctgc
    52741 taaaataaag gaagcacccc aactcggtat tggtgtcttc tttgagaaga gaaaggagga
    52801 acgacgatgc caaaggaaac catacgaaaa taccggtgcg catcctgcga taggaccttc
    52861 gagttcgagg ggaaaccgga aaagtgcccc ttctgccggt gtcgcatctt attcctggtg
    52921 gaaggggaat ccgtgaggga accttcaagc ggaggcggat gtgctgcctc tgggtgagga
    52981 atctgatttt gggaccgtgt gtcccaatcc tgattcgcga acgagcagga ggccgcacgt
    53041 gcctctttga cagcggggcc tccggatgcc ttttcttgcg aagacatccg gaggcccctt
    53101 ctgaaaaccc ggaagaccgg tgaaagcggt cagacccggg aacgcaggaa tttccccaat
    53161 ctctctgctc cgattcgaat tttctcggga ggaaccgcac caaagctaag cctcatgcaa
    53221 tttttcccca tcccgccgcc ttcgatgaaa aagccttccc cggcgacata agcgaccttg
    53281 acatccgggt tcgacgtcgc atctgccaac aactccgtgg tatcgatgcg gcccggcaat
    53341 tccacccagg tgaaaagccc gccgtccggg aaagtccgtt tcgtccccgc cgggaaaaag
    53401 aggtcgatgc tctcgagcat cgcatcccgc cgagtcctgt aaagatcgca gatcattctg
    53461 tgatgggccg gatagtgtcc tctcttgaaa aattcggcgc agatcacctg cggcagcatc
    53521 gaagtgtggg agttcgtggc cgattttgcg tcaaggagtt tttcgatgac cggatcgctc
    53581 gccagaacgt atccaagcct ggatccaggc gaaaagatct ttgagaaact gttggccagg
    53641 acggtattcc ccgtctcgtc gaatgccttg attggtttta ggtcttctcc gctgtagcgg
    53701 atatcccggt atggatcatc ttcgaggatc accacgtcat accggcttcc catctcggcg
    53761 attttcttcc ttctctccag cgaaagggtt ctgcctgtgg ggttatggaa agtcgggatc
    53821 acgtagacga cctttggatc gtagcgcctg atcttctcct ccaggtcctc cgggatcatt
    53881 ccgttctcgt ccatggcaac gctgatgcat tttgcctgga acatttcgaa gatctccacg
    53941 caatgaacga aggtagggga ttccaccagg atcacgtctc ctggatcaat gaacagctga
    54001 cacaacaaat tgatcccctc gagtcctcca ctggtaatta ggatgttttc ggccttcgcg
    54061 tcgatcccct ttggagcgag caactcacgg acgacaactt cccgaaggtc gggcaagccc
    54121 tgaacactgc cgtactgaag tgcctcgact cctcgttttt cgcttcttag gacatcctcc
    54181 gcgatctctc ggacgatatc gatcggcaag gcctcctttg caggagctcc ccccgaaaac
    54241 gaaatcagtc ccgggtctcc catcgctcca aataaattgc ggatgatccg ggcggatttt
    54301 tccattgttt tcatgcgctc agcaaaatgt ggcatggcag cctccttctg gtaggaatct
    54361 tttttccagc ttacgcttct cgaacagcct aacatccgcc gcgagagggc tcaacaggaa
    54421 aaacggaacg tccttgtttc aacgagtgac tttcgcaatt tcaaaaactc cgcaccggtc
    54481 ctccaagcca ggcatcctca gggaacctgt ccgccagggc tttcagttcc tctccttcca
    54541 tgcggttcaa aaggaacctc tcctgaggcc tggccccgat ggacaagtag tattctcgag
    54601 cgggagtgtt ccacgtcagc accccccatt cgagcctgcc cccgtttctt tccaccgtca
    54661 ttttcgcgag gaacgcgagc aaagccgtgc cataaccttt cccccgccag gccggcctca
    54721 cgtataaatc ttccaggtag atcccgggcc tgccctggaa agtcgagaag ttctgaaaga
    54781 agagggcaaa cccgacggga attccttcat ggcaggcaaa aatcacctcc gccgaggggg
    54841 gattcccaaa aagagttttc cgaaggagat cctccgtcgc ctccacttcg tgggccaatc
    54901 cttcgtactc cgcaagctca aggatgaact gcaggatgag ggggacgtcc tcccctgtcg
    54961 ccggccggat tgaaaaccca ggcagcgaaa gcgcccttaa aggcccggca ggggaggttc
    55021 ccggctgctt tttctcgccg ctgtctcctg ccagcgcttc cgtccagctt gcaagccgga
    55081 ccctgccgga tagcgcattc aaggcgatcc ggtggatctt ttcgtccaca gtcgtgcagc
    55141 aatccgacag gacggtcacc gcgtattttt cggcggatct ggaaatggcc gtatgagtca
    55201 cgcaattctg ggtcatcatc ccgcaaacca aaagctcggc agttttgaac cgacctaaag
    55261 tttcctccag gtccgtctcc agaaaggcat ccgcattccc cttgacgaca atgggggcac
    55321 cttgggcaac ggcaaggatt ttcgggtgga tttccacccc cgttgtcccc tccttgaaaa
    55381 agctcgcgcc gctgttttcc gagcttacaa catgctggac gaggatcacg gggataccca
    55441 gggttttggc ttgacaaatc gccttttcca cctgtaccag ggtttcctcg gcttgccaaa
    55501 gagggaactt tccaccggga aaataatctt tctgaacgtc gatcaccaga agtgtttttt
    55561 tcgacacgac ttcccatcct tcccttcttt tgggtggaat tcctgcctct tcgctattct
    55621 acaaccctgc ccgcccgggt catttcggaa ttgccgattg caaggacctt tcgtaaaata
    55681 ccctgggcaa ggaattcccg gcctttttca tccacgagcg ggaaccgaaa ctcaaggtct
    55741 gcaggagggc attggagttg aaaaggaaag tcttaaaatc aaagctgcat cgtgtaaccg
    55801 taaccggcgc agacttgaat tacgagggca gcgtcacgat cgaccctttg ctcatggaaa
    55861 aggcgaatat cgttgaatac gagtacgtcg acatctggaa cgtgacgtac ggcacgcggt
    55921 tctcgactta cgcgatggcc ggccagccgg gcagcggaac gatctgtatc aacggagccg
    55981 cagcaagact cgtttctgta ggcgacaagg tcatcatcgc gacttggcag gaaatcgaag
    56041 aggaggaagc gaaaacccat ctcccaagac tggtctttgt ggacgaaaac aacgtgccgg
    56101 tcgatccgga aagagaaagg gaaattcccg gacaggccga gcggaagatt gcgtcccgct
    56161 gaagcaggga aaacgaggca gggaagaggt ctcacccggt ggatggccgc ttccctgctc
    56221 ctgcacaatc tcctgcgcgg tccattgctc tgctgttcag cccagcatgg aatccaaaat
    56281 cgcctctatg tgaatcttcg ttgtatccag aaccatcacg tcaaggtctt cccttttgat
    56341 gatcagcggc aactcagtgc accccaggac caaggcatcg gctttgcgga gggataaatt
    56401 gcggttcgcg atttccagga ttttcctttt ttcctcctcc aaaacgatcc ccgcttccag
    56461 gttcgggaaa atgatcttgt ggattgcctc ctgctcctgt tcgtccggga caaaagcctc
    56521 gatcccgtac ttttcaaacg ctcgcgtgta aaggccgctt ctcatggtga aagcggtgcc
    56581 gaaaacgaca accctctcgc aagatttgga ttttgcatat ttgcaggttt cttccacgat
    56641 gctgataagc ggcagagggg agtcttttct gacctcatca aaaacaatat gcgccgtgtt
    56701 cgccgcaatc acggcaaaat ctgccccacc ggccgcaagt gccttgatgg atcccaccaa
    56761 ccttcctata aaaggctccc attgcttttt tgcagcgtaa tcgtacattt ccgatagatc
    56821 caggctgtcg atgatcatcc gaggataccg gtcctctcct gtccttttcc ggaaaccctg
    56881 gatgatgccc ttgtaatatt ccaaagtcga ttctggaccg attccaccga caattccgag
    56941 tttcttcatc ctggatcacg cctcctaaga aatgggacgg aaagggctca cttgccccgc
    57001 cccactcaaa cagccgctct tgaccaccct taccgcttta actgggcctt tcagttcttg
    57061 ggcacactcc ggaagtcccc gccttcttgc tttccttgct gcagcattcc agggccttcc
    57121 tgtacatggc aatatcgctg cgctcttcat cgaagtccac atcctcccag gtgatcttcc
    57181 cgtccttcgg aatgtccctc ttcaggatca tttcctccgc caggcagatc gggacccccc
    57241 gttcttcgtc ctgcaaggtc gattcgatca atccgtaaca cgaaaaccct ccgatgccgt
    57301 ctaaccgcgc tcctttcccg agatccgttt ttgcataggc gtacacgttc gaacggaaac
    57361 cgaagtaagg agccagcagg ggctttttca agagtgccgt ctccaggatc gttttcccaa
    57421 cctccacgtg gcagaggtga taaggccgat aaaagaggta aaatggtccc tcgcccatct
    57481 tgtagtaatt catcatgaac ctttggtact cgttctcgca atacccgaca acgaagactc
    57541 cgccgccagg atcagctccc aggatgtaat ccacgacccc ctgccttctc tcccataacg
    57601 tcctgaagtc gaaggcttcg agaacctccc tgacttccgc gaggcgaggc ccggtcatgc
    57661 cagcagtcgt gacgtctagt ccaagagcat tggctaaaag ggccatttcg atgcacaact
    57721 tggtcccatc cgtgtaggcc gtcgtcattc ggcaatccag cctccgcttt tcagcctcgt
    57781 gcgcaatgct gtgcggattg gccctccgat ccaggaaccc cttgatgttc ccggccatga
    57841 cgagctcaaa tccccaaagc cggatgtcat ctacaatccg cttgatgaca ccatgctggt
    57901 cgccgtcgca actggtgtag acaacaccct tttcccttgc cagttccagg aaataggggc
    57961 caaagatgag gtcggcttcg gcattcatca tgattacgtc ttttccgctc ttcaaagcca
    58021 gttcacagaa acgccccgct tccgaaatcg cactcgttgc gtctatcagg atatcgacat
    58081 ccggggattc ggccagaacg caagtgtctt ccgacaccgc catccggcct ttgctcaagg
    58141 ctgctcccac ttccgaaggc cgcccgatag gaaccggctc aaaaccaagc caggaggcaa
    58201 catcgatcgc tttttggatc ctcgtgtcag ccagggcaac gcattgaact ccatcggtga
    58261 atcggcattg ataagcgatc cccttgccca tcgctccggc tcccgcgatg gccaccctga
    58321 tcgttcccgg ctgcttcatt tgatgtctct cccccttttc gatattctca tgattagttt
    58381 actctctctc gatccaaagc actc
//
LOCUS       DAOOVN010000004        45799 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_509_length_45799_cov_14.555155, whole genome shotgun sequence.
ACCESSION   DAOOVN010000004 DAOOVN010000000
VERSION     DAOOVN010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 45799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45799
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_509_length_45799_cov_14.555155"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="PLY83_00935"
     CDS             complement(<1..563)
                     /locus_tag="PLY83_00935"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOV28633.1"
                     /translation="MNLRKGTDPRTALVRDEGVADPLLWLIPLLLSGMGVIMISSSSS
                     PIAMARFGNPQALGMKQAVWILVGMIAMLGSYSIPLQAWRKWSGFFWLAAVAFCFLPL
                     FPVTGVAAGGARRWVNLGVVTVQAADILFLGFTIHASRKLFESRDQETRIGALVKVGI
                     LWGISAVPLILQPDMGSTILVFVLAMG"
     gene            complement(541..1932)
                     /gene="murD"
                     /locus_tag="PLY83_00940"
     CDS             complement(541..1932)
                     /gene="murD"
                     /locus_tag="PLY83_00940"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOV28634.1"
                     /translation="MQNKNASIEGIRIGIVGSGVSGLSLASLAKRKKARVFVSDSGAF
                     RETAAKQLESMGIAFEFGEHGPRLWESDLIVLSSGVAPSAVPVQEARKRGIPLVGELD
                     FVAPYLKGKRVGVTGSNGKSTTTALLGHLLQRMGMKAAAVGNIGSPLADYADLPLDVL
                     VMELSSFQLFWANRLPVDVAVVTNLDPDHIDWHGSYENYVAAKANLLRMQKAGSWAIL
                     QGRDLHFFQEFDAVRKISLGSNEEFPTEGRIVMGEREAVLWTEKGPKRLFTFDSLPLL
                     GKHNLENAAMAMAALEALGFDASTAEPLLGSFSALPHRCEAVATINGVRYVDDSKGTN
                     VAASVTALGSLPGVKLVILGGQGKGEDYTPLAQAVKRHARLALVMGTEREKICDALKD
                     AGFEGIRKVRDMEEAVATAYREALAGETVLLSPACTSWDAYPNYKERGKHFQRLVLGI
                     REGEGLGEPAEGN"
     gene            complement(1937..2908)
                     /gene="mraY"
                     /locus_tag="PLY83_00945"
     CDS             complement(1937..2908)
                     /gene="mraY"
                     /locus_tag="PLY83_00945"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOV28635.1"
                     /translation="MPMNTVAGWSLAAMAAFFTGILEGQWIRLVRRWEVRQVEKSYGL
                     STHLERKSGTPTMGGVVFPIVFCLCLVAFGNLSWQAVPGLLGLPLGAFAVGFTDDWLK
                     FSKRSSEGLKSLEKLALQILVALPWSAYVLLVNRVELFPGISLGLLPAIALLGFCSVG
                     MMNAVNVTDGLDGLAAGASGLSFAGLALLPLIGPGAFETASFVGAGICAGFLWHNAHP
                     ARVFMGDCGSHFLGGFLVSLCVSGGGLMLLVPFGFLFGVEIASVAIQIVAIRGFGKKI
                     FRMSPLHHHFELLGWHETQVVTRFWLVHAAGMIATGFFLEKAMEAWG"
     gene            complement(2899..4296)
                     /gene="murF"
                     /locus_tag="PLY83_00950"
     CDS             complement(2899..4296)
                     /gene="murF"
                     /locus_tag="PLY83_00950"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOV28636.1"
                     /translation="MHRERLRLSEAAAFCGGIVKGPEIPFPGNLWPDSRDVCPGDGFV
                     ALPGVHADGHRFVGAALDNGAGLVIGTAEGIAPWEPRILEAGASCLVVEDAEKALVQI
                     AEGYLQEVSPKVKIAVTGSVGKTTTRELIRSALEPVFRVHSAKRSHNTRIGCALTVLA
                     MPPGTEVLVLEMGTNHPGEIRSIVEHFPPTIAVITAIAPVHLEGLGDLEGVLSAKLEI
                     IPQEGLDLLIYNADDSRLSQAIEGAQRPFRIAGVGYRKGSALLLTGADTVFEEGNLQV
                     GLSYGGQSWVCRSLLKGTHHATNIGFAVMVGLALGVPMEILLKAISGMAPLRGRGKWI
                     RDGRVHLLIDESYNANPSSVAAALEVLRSLAVPGRRLAVLGGMRELGEKSGKLHSEIV
                     GMFDFLDGVVLVGEEWENITEPPAGAKVQQWRVRNAKEAADLLEGLVGKKDTVLVKGS
                     RSYGLEQVVERLSCP"
     gene            complement(4289..5806)
                     /locus_tag="PLY83_00955"
     CDS             complement(4289..5806)
                     /locus_tag="PLY83_00955"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOV28637.1"
                     /translation="MDFRKIIAHLDEEGHFCSAVWGAVSPASELEIERIAHHTAKVTP
                     GSLFCCLPGGRHDGHDYAEEARLKGAVAILCERPLAVDIPQILVDDARKAVNSLAAHF
                     YGYPGKKLKMIAITGTNGKSTTTYLVRSILESAGIQTGLLGTIVYHDGTRETLAERTT
                     PDCVELQEWLSRMVQNGSQACVMEASSHGLVQGRLEGCQFDAVGFTNLTPEHLDYHGE
                     METYFQAKRMLFGEYTRGQWTGAANEDDPYGRRLTEEFPDNVIPYGLVRCGRQTVHAT
                     VRSMDLEGIRLDVHFPSGNVARDWKVPLTGQYNIYNILTATTIAFGLGLHEETIRLGL
                     ERVPQVPGRLERYLFENGVCCIIDYAHSPDALRNVLKTLGDVVRGKIWSVFGMGGDRY
                     APNRPLMGEVASELADELVITMDNPRSEDPGEIARQIEGGLKEGLRLKTHRIILDRPE
                     AIHYALDHARPGDVVLVSGKGPEPYIIIKDQTLPYSDKETVMNWAKARGLEFPHA"
     gene            complement(5837..7426)
                     /locus_tag="PLY83_00960"
     CDS             complement(5837..7426)
                     /locus_tag="PLY83_00960"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOV28638.1"
                     /translation="MFVQAWPDPRTVLQANSQYWTQVAISTSRGVIRDSRGSALALSI
                     PATSLFIDPQYWDPANAAVVKNVLGDLISPEVLEKISRPMKGRFVWVARKIPPEVAAR
                     ILSKKLEGIYALKERKRVYPQGKLLAHVLGFCDIDDEGLAGLEQSWQEILYSPPQVKI
                     LARDAAGQGIDLLSLQRAPKQHGPGELTLTIDPILQMVVEKHLEEATVEYRPKWAAAV
                     CMDPRSGAVLASASWPTFDPNRRETLLDPAVRLNNVIGRVYEPGSTLKPVIMATAMEQ
                     GLVRENEAFHCAGRIRIADGTVTDIKAHGSEILQDLIVNSCNVGMAQIGIRFRPRFAY
                     DNLRQWGFSDPSGIELPGEEAGLVSTPEQWRGVVPANIAIGQGIGVTPLQLVAALSAV
                     INGGRCVRPFIVKEARDGTGNVVYRGEVCVRNVVISKKTSDWVRSAMRRVVTNGTGKA
                     ANSPHVTVGGKTGTAQVAERGKYVKGRFVASFVGFWPAENPDHILLVVLGEPSQKGHL
                     GGAIAAPAFRAILEDYLLLAR"
     gene            complement(7541..7960)
                     /locus_tag="PLY83_00965"
     CDS             complement(7541..7960)
                     /locus_tag="PLY83_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV28639.1"
                     /translation="MPSPVGNRKRMQRKGRAHSAFAAVAVFVGVLVMGLGGLRIYNMS
                     LEQRLTRLNETIENYQELELDLRNKLASLLSPSRVYSVASAKMGMVSDAGVAVVTVPP
                     AARPAQSVALRPISGQKAPVAGFSLAGLFQKEAHAQD"
     gene            complement(7967..8887)
                     /gene="rsmH"
                     /locus_tag="PLY83_00970"
     CDS             complement(7967..8887)
                     /gene="rsmH"
                     /locus_tag="PLY83_00970"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOV28640.1"
                     /translation="MIEHIPVLLEEVVTQLSRECPPAFIVDATVGLGGYSERFLLEWP
                     SCRVLGIDQDPMALKRTRERLSGPLAAGRFRVVEGNFRCLQQLVSETGWGRPDGVVFD
                     LGVSNLQLSLSERGFSFQEEGPLDMRMGRGETTAEELINKLSEAELRRLFRDYGEEPH
                     AAPIARGIVKHRDQKGPIRTTAELVNVIRNALPAPLQRKMNRHPARRVFQALRIAVND
                     EMEALREGLNQAVRSIGENGCVLVVSYHSLEDRIVKHAFNQWEKDGLGRRMPRKAIQA
                     SPEEVQRNYKSRSAMLRVFRMNGKEIDPGR"
     gene            complement(8884..9309)
                     /gene="mraZ"
                     /locus_tag="PLY83_00975"
     CDS             complement(8884..9309)
                     /gene="mraZ"
                     /locus_tag="PLY83_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOV28641.1"
                     /translation="MLVGTFEHRLDSKGRLVIPARFREELGDSVVASFGIDRCVSIYS
                     LKSWEGILEKLHNLPYSKGKTRDLIRVLLASAHELPIDPAGRVLLPQSLREQAGIDQE
                     VSINGVLDHLEVWDSEQWNRYRTRVLETLPEIAEEVDGF"
     gene            complement(9515..9863)
                     /gene="rnpB"
                     /locus_tag="PLY83_00980"
     ncRNA           complement(9515..9863)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLY83_00980"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(9900..10415)
                     /locus_tag="PLY83_00985"
     CDS             complement(9900..10415)
                     /locus_tag="PLY83_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOV28642.1"
                     /translation="MDKRKFPCEWKLETAEAAFVAASAVLVGSVFLEKDSSVWYGSVL
                     RGDLNSIRIGSRANIQDGAVLHVTLERGVRIGPDVTVGHGAILHGCVVDRECLVGMGA
                     ILLDGAQIGEGSVVAAGSLVPEGQKVPPGSLVMGVPARVVRSIRPEERDRIRELAASY
                     VELAFLHASLK"
     gene            complement(10428..11054)
                     /locus_tag="PLY83_00990"
     CDS             complement(10428..11054)
                     /locus_tag="PLY83_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOV28643.1"
                     /translation="MAKRLNVNPNRMELSRLKKRLVVAKRGHKLLKDKQDALIKEFLG
                     RARAIKLLREQLEGELAECYRSFLLARAQTLPAMLEQSLMISGASCSLEVDYRNVMSV
                     IVPEYTVNQGGGTLSYGLATSPGSLDIALEKFSRLISRLIKLAADEKALKLMASEIER
                     TRRRVNALEHVMIPSFKETIRDITMKLDEMERSSQSRLMVIKEIVRSH"
     gene            complement(11067..12485)
                     /locus_tag="PLY83_00995"
     CDS             complement(11067..12485)
                     /locus_tag="PLY83_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOV28644.1"
                     /translation="MLPREYRTITNLSGPLMVVEKVNEVRYDELVEIELASGERRRGR
                     VLEITTDKALVQVFEGTTGIDCESTKVRFLGKVLTLPVSRTMLGRIFNGRGEPLDGGT
                     GVIPEALLDINGNPINPYSRDFPSEFIQTGISTIDGMNPLVRGQKLPIFSGSGLPHNR
                     MAAQIARQAAVISGHEAFAVVFAAMGITFEEASFFMEDFRKTGAIERTVMFVNLADDP
                     AIERITTPRLALTAAEYLAFECDMHVLVILTDLTNYCEALREISAARKEVPGRRGYPG
                     YLYTDLATMYERAGRLRGRKGSITQLPILTMPEDDKTHPIPDLTGYITEGQIILSRGL
                     HRKGIYPPVDVMPSLSRLKDKGIGKGKTREDHADLMNQLFAAYARGKEAKELAIILGE
                     GALTEEDKAFARFSDAFEDRYVRQGEYENRTVEETLGLGWELLTMIPVRELKRVRDAY
                     VEKYLKPLLKEREANESEAVLS"
     gene            complement(12475..14268)
                     /locus_tag="PLY83_01000"
     CDS             complement(12475..14268)
                     /locus_tag="PLY83_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOV28645.1"
                     /translation="MAVGKSVKGKISKISGPLVVASGMTGAGMYDVVRVGNLGLVGEI
                     IELKGDLASVQVYEETSGLMPGEPVISTGAPLSVELGPGMIEQFYDGVQRPLNAIEEK
                     AQSPFISRGINVPAIDRARKWTFRAKVKKGDFVVSGDVLGVVQETVLVEHRIMVPPGM
                     QGRVEEIREGDFTVEETIAVLIDGEERREVQMLQRWPVRTPRPVARRLPPVVPLTTGQ
                     RVVDMFFLIARGGTACVPGPFGSGKTVIQHQLAKWADAEIVVYIGCGERGNEMTDVLL
                     EFPELEDPRSGQPLMKRTVLIANTSNMPVAAREASIYTGITIAEYYRDMGYSVALMAD
                     STSRWAEALREISGRLEEMPGEEGYPAYLGTRLASFYERAGRAICLGKAGVEGAVSVI
                     GAVSPPGGDLSEPVTQNTLRVTKVFWGLDASLAYQRHFPAINWLSSYSLYAEKLGEYW
                     DGIYDGEWAKNRIEAMSLLEDEDRLKEIVRLVGIDSLSKEERMILETAKSLREDFLHQ
                     NAFHEVDTYASMDKQFKMLKNILTFHHLAMEALKRGAALKDITDMAIRERITRMRYVD
                     EKELQLLDQLEEDIKKEIGNLMPAGGEVHVA"
     gene            complement(14324..14665)
                     /locus_tag="PLY83_01005"
     CDS             complement(14324..14665)
                     /locus_tag="PLY83_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HOV28646.1"
                     /translation="MSAVSRNRSMAALGDYESVLPFQAVGIEPHVLEDGADAEAKRIL
                     NELVQGKYAVVFVVEDWYERLKERIEEINEKEDTSIVPIPGLRGSRGLGFKSIKSSVE
                     RAVGMDIFSVK"
     gene            complement(14652..15668)
                     /locus_tag="PLY83_01010"
     CDS             complement(14652..15668)
                     /locus_tag="PLY83_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HOV28647.1"
                     /translation="MAQAESYGYAVARLRAMENRLLDSGAFQRMLDAEDLQGALKVLN
                     ETCYSSWLVEMKNEGAFDGAIEAELNYVYRETAQFVPDKALVTLCKMPYDFHNVKVLL
                     KSSFLQKSGGPRRWDLLTRLGNVSPEFLIESLESEDYRLLPFGLRSAVPQSLAQWEQF
                     HDILEIERQLDDLLFSEMLKLAAKLERKGIVEWVRGRIDAENVRNLLRLRRMQVENNR
                     IAGFLHEGGIITVERLLSLTSEPLESWGRLLAYTDLTGALSQLQEISDFDRLIVDVEK
                     ALDDYVLRVVEKTRYSSTAPEYVLYYLWLKEMEAKNVRIILVGKANGANRDVLRGLLR
                     HVSG"
     gene            complement(15683..16264)
                     /locus_tag="PLY83_01015"
     CDS             complement(15683..16264)
                     /locus_tag="PLY83_01015"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HOV28648.1"
                     /translation="MSLTDIKAKIEEDARAEAEEILSSARSQAEKIHRQTEEEIRRME
                     ETQKKRLEAEIPEILRRKEIVAKLDVNNQDLTARRTLIDEAFEAAKGLMCGMKADEAR
                     RFAETLLLKAVETREEWLLVGRDEKALDATWLQQCNEKHGWNLKMAEEKAPITGGFIL
                     RRGLVDVNCSWEMLIRTAREELEAETVKKLFSN"
     gene            complement(16279..16791)
                     /locus_tag="PLY83_01020"
     CDS             complement(16279..16791)
                     /locus_tag="PLY83_01020"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOV28649.1"
                     /translation="MDQVVATLLNTMSEQLGAMIAVLGAGIAAGFAGSGSAIGIAIAD
                     EAAAGVMTEDPGKFGLALLLLALPGTQGIYGLLAAFFAILKVGLLGGAAVKVTIWQGI
                     GIFGSLMPIAIACYYSAVAQGKASAGAILLISKRPEEIGKAVILPAMVETYAVLALLL
                     SIILMNSIKL"
     gene            complement(16836..18869)
                     /locus_tag="PLY83_01025"
     CDS             complement(16836..18869)
                     /locus_tag="PLY83_01025"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HOV28650.1"
                     /translation="MALVEMKKVELALHRSVTDRVIASLQRLGCCEIIRGGPDSASEK
                     AGGVTPSDELKRYDNLLADARFALRFLETYRGEKAGFLSRALAEKPQHSMSELESWSK
                     ETDFQQLILHLRNLERQFVEIRTELSQIAGLEGILRAFGELPQPLFFFTTGTERVKGI
                     LGTLPADQVDRLEVAFSAHVGKEGELFVRINNPEKDKDAKAALVYLRENEEKVASICS
                     EVSFNRIDLPRDLSGTVGEEIARLEARKELLVKRETDLQNQARSLAAEWLSRVQALSD
                     YWSVMKGRYEALDSSERTEQSHFIRFWAPAESLEKIKKELEPFSSLSETTVKAPEEGE
                     VAPSLLKNPAWASPCEPLTNMFGVPTYGGVDPTLMMAPFFYVFFGMCLGDAGFGIILS
                     ALLGWVLLKFPVGGNLRKLLVVMFICGLSTIVMGALTGSWLANTIDSFAFLHFLRPLR
                     DRLVVWDPMNDPMTYLGVCLALGFVQLIFGLCIAFWDALRRKSYLEAFADRGGWILLL
                     NGVALFFLVMIGKAPASMGMLAKSLSIAGAVTLLLTQGRSKKGILGKAFSGALSLYGI
                     TSYLGDTLSYSRLLALGLSGAAIGLIINLLAKMISEGVPYVGWILALILFVVGNIFSL
                     AINVLGAFVHSLRLQYVEFFSKFYEANGRLFSPFCHETQFVKIVDSCGQQEKQ"
     gene            complement(18856..19191)
                     /locus_tag="PLY83_01030"
     CDS             complement(18856..19191)
                     /locus_tag="PLY83_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein TolA"
                     /protein_id="HOV28651.1"
                     /translation="MKAMATSLAEEIRQAEENAKKIIQQAKAEASRLVSDARSESERR
                     AKEAKQELHQKFRQEIAEAEAQAEKRAETIRAEGKKQTEAFVQARKSRVEEVATWLVK
                     EVTAKYGLS"
     gene            complement(19368..19443)
                     /locus_tag="PLY83_01035"
     tRNA            complement(19368..19443)
                     /locus_tag="PLY83_01035"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19408..19410),aa:Gly,seq:ccc)
     gene            19417..19539
                     /locus_tag="PLY83_01040"
     CDS             19417..19539
                     /locus_tag="PLY83_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV28652.1"
                     /translation="MLYQLSYVRICGGYYIDEVLLWQGKFFEIARNSRTALQDC"
     gene            19670..20086
                     /locus_tag="PLY83_01045"
     CDS             19670..20086
                     /locus_tag="PLY83_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOV28653.1"
                     /translation="MWTLDDGIARLKESGTKITAQRIAILKRLEGRRDHPSAEQIYHE
                     LKRDFPTISFATIYSTAQLLAEKNLVQILTIDDKRVNYDPNPEPHGHFRCTACGHIED
                     VPITESLLEMLKMGQDSTISSVQLYMYGLCPSCNVR"
     gene            20420..21532
                     /locus_tag="PLY83_01050"
     CDS             20420..21532
                     /locus_tag="PLY83_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV28654.1"
                     /translation="MKTDAAHHPRELSPLQKLLVGLVLLYGLVFTPASAQAATPQLLY
                     SILLPFQVGGTVKVILPDGSEQPIGTVEMLPEKTRWPAYTATRWGTPGTVAASAVNAI
                     HLLIDIEKGRGRTLSLLPSETIAPAAGPGSALVVTGKGGYGLFGGWAPPVGSPVTVIS
                     ASGEERPLNGGSIPREGETLRIEVNNLCSPYMIEIENRPGGRVFAWSRAAEQSGVIAR
                     VIRPVRGVGRFEGTLFQSVGRLRANHPGVIDISTSPEGAIGGFQIIPFNHAHSAEMEG
                     AWQRTQWLIIDSPDGKTPLTGRPPLFGGILVPGPRETERLWDLWSTYGRRPLVLCRID
                     GGPWTRLPEVIGKQDLALENVTHLRLYFPVVEEPNL"
     gene            complement(21613..23439)
                     /gene="glmS"
                     /locus_tag="PLY83_01055"
     CDS             complement(21613..23439)
                     /gene="glmS"
                     /locus_tag="PLY83_01055"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOV28655.1"
                     /translation="MCGIVGYVGMRNAPDVLLEGLKRLEYRGYDSAGLAVKSDGRIEV
                     IKEVGKVADLARLVEERQLEGCQGIGHTRWATHGGVSVDNAHPHCDDCGNFVLVHNGI
                     IENYLDLREELEAAGVSFTSETDTEVIVQLLARLYRKDMLDALLKLTEKLRGSYALAI
                     MNREVDGTFYCLRKGSPLILGIGEGEGFCASDVPALLPYTRQVVYLEEGEIAAVSRDG
                     IQVWASDGQRVEKSISTIDWEVSMTEKAAYPHYMLKEIHEQGAVLRTTLKGRVENGKV
                     DLSPELAWDKELAQSWRKLHIVACGTSYYAALVAERVLEKWTDLEVKVDIASEYRYRH
                     LRADTETLAVFVSQSGETADTLAACRLARLQGAHCVAVTNVRGSTLAREVHDVLLLKA
                     GPEIGVAATKTFMGQIAALYLFAIYLGRLRETLSAAEGNRLLEEMLRLPYKVEVTLER
                     EDSVRALAEKYADRKDFLFLGRGMSFPVALEGALKLKEISYVHAEAYAAGEMKHGPIA
                     LLEAAVPVMVIIPRDGLYEKTLSNIQEAKARKAPILAVATDGDDLISRHANDVLRVPW
                     TDEEMTPFLTVVPLQLFAYHMARLRECEIDQPRNLAKSVTVE"
     gene            complement(23658..24539)
                     /gene="galU"
                     /locus_tag="PLY83_01060"
     CDS             complement(23658..24539)
                     /gene="galU"
                     /locus_tag="PLY83_01060"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HOV28656.1"
                     /translation="MSVRHPLRKCLFPVAGLGTRFLPATKDIPKEMLPLIDRPIIHYG
                     VEEAVSSGCRDIVFVTGRSKRSIEDYFDRSVELENLLERRGQLGLRDELARISEMARF
                     AYVRQSEPLGLGHAVLCGEPCFNDSFFGLILPDDVILSDQPVLGQLAEVHESLGGSVL
                     ALEEVPWEETSRYGIIEGYPSGQGVFRITNLVEKPSPGQAPSRFAIIGRYLLSPSIFG
                     CLRSLQAGVGGEIQLTDALQALLAEEPVYGLLYRGKRLDCGTKLGWLKATVTLALHDP
                     ELREAILSIVRKEECND"
     gene            complement(24544..25932)
                     /gene="glmM"
                     /locus_tag="PLY83_01065"
     CDS             complement(24544..25932)
                     /gene="glmM"
                     /locus_tag="PLY83_01065"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOV28657.1"
                     /translation="MSETGKIRCLFGTDGVRDIANRGLMTPEMALSLGRAFVLFMTER
                     GIPRPTVALGRDTRKSGCMLEAALSAGLTSAGADVLSLGVIPTPGVSYTVRNMGIHGG
                     AVISASHNPAEYNGIKFLDSKGYKLSDDDEAAIEEYLGDPLMDEWRPSGASIGVVRQS
                     PESILRYRDWLMGLFGEGWLSGKVAVIDCANGAASWIAGELFSGCGLDVRMVGNCPDG
                     LNINEGVGVMHMPYLARAISASGAHFGIALDGDADRVLLADSKGRIIDGDILLWVLAR
                     WLQKKGTLGSGVVSTVMSNMALEERLSETGIPLTRCPVGDRYVLEEMKKSNSRLGGEQ
                     SGHIILYQHVHTGDGLCTALNFLKACEELGEDLDTLVDRFARFPQLLRNFRVETKSGI
                     LESEGVQKAIRDCQLRLGDKGRVFVRMSGTEPLVRILVEAREESLLSEITDRILETIR
                     AAGAESASERKG"
     gene            complement(25961..27190)
                     /locus_tag="PLY83_01070"
     CDS             complement(25961..27190)
                     /locus_tag="PLY83_01070"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOV28658.1"
                     /translation="MAFKRIESLLSSKMFLRAVSLIVALFMWFYVSGDRGYEVNRTLR
                     LKVNYQNASPGFSILGGEKEVEVRIAANRKTFESLELDKTECAVDLKGLEGGKYRLPV
                     RVLLPPGVRLVSVNPEQVTVELSRLIEKVLPVKIVWEGEFPEDVLLEKLEVTPSEVTL
                     RGPENVLSSLNEVFVQATPAKVLSGEEITLEVKPRTGKGESLPRGADLYPGVIRLKAI
                     LVKDVEKKELQVVPTVSGVPEAGYRIEETVPQPPAVQVVGARSVLEKLGPVETKPIDV
                     TGLAQDKTFETEPILPEGQIKLLGSGKIKVSVIVRPVENGTGSLRLKIPVKVEGKSLY
                     SSWKAVPSSVAVQVVSESGSSAFQNQSEPLVEAYVDVTNLVSKRIVVPVQVRVLSKDV
                     RVLKVEPERVVLYTVSP"
     gene            complement(27180..28007)
                     /gene="cdaA"
                     /locus_tag="PLY83_01075"
     CDS             complement(27180..28007)
                     /gene="cdaA"
                     /locus_tag="PLY83_01075"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOV28659.1"
                     /translation="MLENFRWQDLLDIFIVAFILYRVLLLMVDTRAMQLVKGILVIGA
                     TAAVSKYFDLKALSWLLERALGAIFIAIPIVFQPELRRMLEELGRGKIWKRRKAIERA
                     EQMADDVTRAISFLRDQRQGAILVFQRETGLADVWRSAVRLNALISQELIVSIFWPNN
                     PLHDGAAIIDRSVIVAAACYLPLTSNTDLSRWYGTRHRAALGVTEVSDAIALVVSEER
                     GEVALATNGHLSRGLKEDQIHRLVYHYFMAMEGPKGLWDQLREEIKALWREEGQDGV"
     gene            complement(28111..28953)
                     /locus_tag="PLY83_01080"
     CDS             complement(28111..28953)
                     /locus_tag="PLY83_01080"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HOV28660.1"
                     /translation="MSLIGLHVSISGGFLKAIERGKAFGCEAIQIFTKNQLQWKAPSI
                     QPSQGISFLKALRESGIRKVVAHASYLINLASEGEQRRKSVEALIGELDRCDQLGIGE
                     LVLHPGAHLGIGEERGVENLLEGLRYVLAKTSHLKVKILLETMAGQGTVLGARLELFR
                     EVLDGLDWDERLGLCLDSCHLYAAGYEFRERSSYEHFARSLEKAFGLSRIGCWHLNDS
                     MAERGSLRDRHQHLGDGEVGLKFFSMILNDDRWENVPLILETPKAGIGDAGNMALLRK
                     LRGY"
     gene            complement(28944..29543)
                     /locus_tag="PLY83_01085"
     CDS             complement(28944..29543)
                     /locus_tag="PLY83_01085"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HOV28661.1"
                     /translation="MLWERRLREEIPAGCPDWEAIPTEGKFRQSAVLVLFCGPEEDTR
                     ILLIRRPRHLNRHPGEIAFPGGRREPFDRGPFETALREAQEEIGLDPARIRFLGLLER
                     EYAYTSDFEIQPVLAFQEAGAVSFPRLDPNEVDEIFLPRVEEFLSPPRLEWVKHGTVQ
                     VLFPVFELPGAQRVWGATARILWRLGRVLRETGVENPCL"
     gene            complement(29562..31319)
                     /gene="pyk"
                     /locus_tag="PLY83_01090"
     CDS             complement(29562..31319)
                     /gene="pyk"
                     /locus_tag="PLY83_01090"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOV28662.1"
                     /translation="MKKVKIVCTLGPACRSRETLTEMVKAGMNVARLNFSHGSYESHG
                     ENLENIRTVEQQLRTPIATILDTKGPEIRTGKLVNGLPVTLVRGQFFRFVAQEGMGTE
                     NQVSISHPQVCRELQKGQDVFIDDGSLHLKVLEAGETELLMEVLVGGELGEQKGVSFP
                     GAKLKIPTLTDKDREDIRWGLEHGLDYIAVSFVRTRDDIMEVRRVMEEMGGSMKIIAK
                     IETSEAVASLQEIVEVVDGLMVARGDLGVELPAEEVPLIQKRMIDLGRSQGKPVIVAT
                     QMLDSMIRNPRPTRAEVNDVANAVLDGADAVMLSGETAKGAYPVQAVETMARIIARTE
                     KEIRVWQRPSSLPPVASQTVPDAVSHAAVTIAEQMQAKVILSLTQSGSTARMVSKYRP
                     GCPISGVTAERRTWRELALVWGVVPILTGKTHADEREMVESGLLATLAEGMASEGDIV
                     VVTSGLPTGIAGTTDMVRVHTVGRIVVKGISLVRKEAYGPVVKGTTAAEARSKMRPGA
                     VLVARQTDRDYVDAIRKASAIICEEGGLTSHAAILALEMGIPCVVSATDALKLLEEGM
                     FVTVDGSRGLIYHGKVTVR"
     gene            complement(31306..31566)
                     /gene="mtrB"
                     /locus_tag="PLY83_01095"
     CDS             complement(31306..31566)
                     /gene="mtrB"
                     /locus_tag="PLY83_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp RNA-binding attenuation protein MtrB"
                     /protein_id="HOV28663.1"
                     /translation="MREEAGEIASVLSDYIVIKAMENGVTVIGLTRGQETKFSHTEKL
                     DAGEVWISQFTEHTSAMKIRGAAEIYTKHGKILCGRLHDEKG"
     gene            complement(31591..35001)
                     /gene="dnaE"
                     /locus_tag="PLY83_01100"
     CDS             complement(31591..35001)
                     /gene="dnaE"
                     /locus_tag="PLY83_01100"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOV28664.1"
                     /translation="MKDPFVHLHVHSEYSLLDGAIRCSEIAARVAEWGSPAVALTDHG
                     AMYGTVEFYEKCCACGVKPIIGCEVYLDPLGHTCRDRKGGNNHLILLARDLEGYHNLV
                     KLVSIANTDGFYYRPRIDHDLLARHSKGLIGSSACLAGEIPALILEGNEEGAAERAIL
                     YQDILGKGNFYLEVMHNALPEQAVVNKAIVRISKATGIPLVATNDAHYLNASDASWHD
                     ILLCVQTNATVNDPNRYRFGASEFYLRSPEEMWALFGKELPEALTHTLEIAEKCDVKL
                     EFNVYRLPRFEVPEGDTLESYLERLAWDGLNERMGGSPPQEYRDRMSYELGVIKKMGF
                     AGYFLVVSDFIRAAKARGIPVGPGRGSAAGSLAAWALKITELDPIRYHLLFERFLNPE
                     RISMPDIDTDISDKRRDEVLEYVVRKYGREHVAQIITFDRMKSKGAIRDVGRALGIPL
                     PDVNRVAKSVPDGVGSIAEALEGSPDLALFQKKEPAIRNLLEVAGKIEGLARHCSQHA
                     AGVVISPVKVTDLVPVRKIGDDQVVTQFGMEAIEKLGLVKMDFLGLRTLSMLEDTLEN
                     IRLNGKEIPDINALPLEDPSTFALLQAGDTLGVFQLESPGMRQLLKKILPDSFEDLVA
                     ILALYRPGPLESGMVDQFINCKHGRSEVVYPHPLLEPVLKETYGVILYQEQVMQIAAT
                     LAGFSLGEADLLRRAMGKKKMEVMEQQRKKFVDGCLSNGVERTTAESLFDLIQKFAGY
                     GFNKSHSAAYALISYQTAYLKAHYKAEFLAAFLSSQIGSRMDVLARYVREVRNAGIQV
                     LPPDINESGAAFSAVRGGVIRFGLKAVAKAGETAVEAILAARQSKGAFTSLWDFLNKV
                     DLRIVNRSVIENLIKAGAFSGLHQNRKQLLENLPLMIDRVQKKQSDGAQRSLLTLVEE
                     LPQEEPVMGECDDFTLLETLDLEKEVLGLFISGHPLEKEEERIARFSTCRISDLRFWK
                     APESQATVGGLLVSFKEKTTKRGDLMGILEFEDTESRLEVVCFPKLWQSVKGILDPGK
                     IFFARGKVQDRGGITMVADEVIPLSEIENSQEPFVRIRLKGETGPLPFKALCRELKGH
                     AGTSPVIVEYFGENSMTVIRLKEIRVKPEASLVERLVNLCGEGLEVIL"
     gene            complement(34998..36785)
                     /gene="dnaX"
                     /locus_tag="PLY83_01105"
     CDS             complement(34998..36785)
                     /gene="dnaX"
                     /locus_tag="PLY83_01105"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOV28665.1"
                     /translation="MSEKGIFSSPVRYVSLYRRYRPLRFEEVVGQDAAVSVLRRSLQT
                     GKTVHAYLFSGPRGCGKTSLARILSKALNCTDDNHGAEPCGSCPSCLAITAGESLDVV
                     EIDGASNRGIDEVRELKAHVSLAPFSSRRKVYIIDEVHMLTEPAFNALLKTLEEPPSH
                     AVFILATTEPHKVPVTIRSRCQHIPFRRISGKDIATRLSLVCEKEGITADEAALWELA
                     RQADGAMRDALSLLEQAIPLGSGVVTIDSVRALLGGGSRCEMERFTASFRTTPDLAVS
                     ELMESLRKGTSMERILENLFLIFRDLWILKAWGETALEGLELSGEERAFLIGEVPFWT
                     LEGLRACLDTLSRLISRVRTGMRTDVLAGLLLEGLGRAALASGPISEGAAMPLQQERT
                     LPRDERIPRPSPVVPDPETQPSSDALPAIDIDAIRRFWIGLLESRDPEDRQIVAAIAP
                     ARVLAGKGGVEVDFPQRDRICYELIRLERNRRRFHVLKEGALGEIPFIFCCGKDRFLE
                     KETPAMETVPAGEDPNGIPWLRRDQADDSERGKRIQETRPEAEPARGASRNATIEEML
                     SWFGGEILLVRTDDQEGGIGGFEEGSAGE"
     gene            complement(36804..37889)
                     /locus_tag="PLY83_01110"
     CDS             complement(36804..37889)
                     /locus_tag="PLY83_01110"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOV28666.1"
                     /translation="MRKKTFVFLVLIALFAVSPPQAMGALSKEDEREVALGKKVCEQV
                     EKKWERVTDPVPVARLAAILQKILPEARKPFPYEVRLIREKQPNAFSLPGGFIYFTTG
                     MLDFAKSDAELASVMAHEMIHADNSHGMIQSARNERLSLLALAVAVASRGEAAAMIMA
                     NIAQVAILNAYGRDLEKEADLGALRILHATGFPVAASVTVMERLAEEQLKHPYVNPGV
                     FMDHPEIPERIGYMVKFIRNSGWALHRKEPLHLLRISIESQEDRFVMTVDGKAIWRGP
                     VGEKVRVLMEGVKRGLDQSLQMELMSNEIEVRPDGKSRVLRVGQKAVVRESDLPAGMD
                     SLDVLSENLKKALIEAQRVHPMGHYLL"
     gene            complement(38009..38461)
                     /locus_tag="PLY83_01115"
     CDS             complement(38009..38461)
                     /locus_tag="PLY83_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HOV28667.1"
                     /translation="MTRPYRILLVCTGNTCRSPMAEGLLRKLMRERGIQGEVSSAGLF
                     TVDGLPASFYAIEVMKEWGIDLSGHRSRQLTHTLVKRADHVVGMTAEHVASLLAQYPE
                     ELSRIRTLELPDIPDPYGKPLETYREVRDLLRDWGHCLLDSLFDCIAG"
     gene            complement(38470..38928)
                     /locus_tag="PLY83_01120"
     CDS             complement(38470..38928)
                     /locus_tag="PLY83_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOV28668.1"
                     /translation="MRICEVMDRDLTALSAETTLSEAIEILTRHRVSGLPVVDGAGRV
                     VGFVSEKDIVKAALPGYFDYLQDPSFIPDYGQFQKRLKKIGKDPVSKIMAKEIVSFSE
                     EDSDFYVAITLIRNHLKRAPVVRDGILVGVVNRADLLERIMLEEESPSFA"
     gene            complement(38944..39876)
                     /locus_tag="PLY83_01125"
     CDS             complement(38944..39876)
                     /locus_tag="PLY83_01125"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HOV28669.1"
                     /translation="MIVTVTLNPAIDEEYVVPEFRPGGWFRVSRVQRSPGGKGINVSL
                     ILKQLGVETAAMGFLAGFSGEYVRDVLRKAQITTNFLHVRGETRTNVYIVDEVGKVET
                     GLAELGPYISEDEIARFLSNYERILKRTELVVLGGSLPPGVPQDFYCELIRLAKQREI
                     PTLIDAAGAPLLAALDEGPTYAKVDQRFMATYEGVALNSLDNLIEAVQRVHSHGVEWV
                     IASYRTYGNVFCTHEGVFLAEFDRKGIVSMFGSGEALIAGLILGGQERMETKDRIVFG
                     MAAALENALHVEKGIRDRAAVERQIPRIRFERLL"
     gene            complement(39910..41100)
                     /gene="trxB"
                     /locus_tag="PLY83_01130"
     CDS             complement(39910..41100)
                     /gene="trxB"
                     /locus_tag="PLY83_01130"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOV28670.1"
                     /translation="MEKRELVIIGAGPAGLTAAIYGRRSGLDVLILEKGLPGGQINTT
                     DEIENWPGVKHASGQELADMFREHAEKFTPEFREAEVEAIEFKDGKKIVVTDKGPVEA
                     QAILLATGASFKKQGCPGEAEFTGRGVSYCAVCDAAFFEDQEVAVIGGGNTAVEEAVY
                     LTQFASKVTIIHRRDSFRADRIAVERALSNPKISVIWDSVVEEIRGTDMVESLSLKNV
                     KTGEKTVLPVSGVFVFIGNEPNIGFLKPLIDSGRLKTAKGGWLRVNERMETSIEGLFA
                     AGDLIDKYLRQVVTAAGDGATAAMAAYAYISEQVHLEGKLLEPEEVTAVFFSSVDEAQ
                     VRLAAEIEASPEWNRVVLVDGYKNSRMAEKLQVQSLPSALKIRKGEVLKRLQIHSAEE
                     LKGL"
     gene            complement(41183..42025)
                     /gene="prmC"
                     /locus_tag="PLY83_01135"
     CDS             complement(41183..42025)
                     /gene="prmC"
                     /locus_tag="PLY83_01135"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOV28671.1"
                     /translation="MNRGQVRFEVRERLRKSGIPLAALEADILCSEVFGCSREDLLAH
                     PERQVKPEEVHRLFSLAEARIARVPVAYLKGRASFWGLDLLVGRGCLIPRPETELLVE
                     TALELFSKGRFLDWGTGSGCLALALLAEREEAMAVMMEKSPLALAWAWRNLVRFPFQH
                     RALLWHGSSIRQIPSAWLPFDLIVGNPPYIPSSEIPSLMEDVRLYEPILALDGGPDGL
                     QQVRNILREAESALLPGGWLLLEIGGEEQAERLKTVSSPALELAWVKRDWAGKDRIAV
                     WRHR"
     gene            complement(42039..43112)
                     /gene="prfA"
                     /locus_tag="PLY83_01140"
     CDS             complement(42039..43112)
                     /gene="prfA"
                     /locus_tag="PLY83_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOV28672.1"
                     /translation="MDFVKKLEEVEKTYIELERLMTLPEVVGNPQELQRVAKKHSDLS
                     EIVGAFREYEAVRRELQEARELFDTDESEMKEMAREEIERLEAVLSELEKRIGLLLIP
                     KDPNDDRSVIVEIRAGAGGEEAALFAANLYRMYVRFAERQGWSTELLTLNETGIGGFK
                     EVAFRIPNKGAYSQLKYESGVHRVQRVPVTEASGRIHTSTATVAVLPEADEVDVQINP
                     EDLKIDTYRASGAGGQYVNMTDSAVRITHIPTGVVVSCQDERSQLKNRVKAMAMLRAK
                     LYDSALQKQQESLASERRGQIGTADRSERIRTYNFPQNRLTDHRIGLTLYKLDQVMDG
                     DLQELIESLNLADQAEKLKNLES"
     gene            complement(43133..44083)
                     /locus_tag="PLY83_01145"
     CDS             complement(43133..44083)
                     /locus_tag="PLY83_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOV28673.1"
                     /translation="MKNLRAGCLFLLGSLTAVGESAEKPERLPVGGQAVIEGVLMRGR
                     GQWALAVRKEDGTLFEKVWPNVPWAAAGIWKLPILRGLATMAEMLTIGYKALALSAEV
                     ALGEDGQVSAKEMALTVGIALSLVIALFVAFPVWLTDRLYGQDWGNAWEGRILEGLIR
                     AAVFIAYIAVIGLWKDMRRVFEYHGAEHKVINAYEAGLPMGTFRSLRSSSRFHLRCGT
                     SFLLVIVAVSIVVFALAGNGSLFWRVGCRILLFPLVIGVAYEIIRGASSIPRAGKWLL
                     FPTLWIQYLTTREPGEEQLALREKPLNWHSETKRGRARPL"
     gene            complement(44070..44765)
                     /gene="thyX"
                     /locus_tag="PLY83_01150"
     CDS             complement(44070..44765)
                     /gene="thyX"
                     /locus_tag="PLY83_01150"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOV28674.1"
                     /translation="MPPLVRLITWTPEADRIVAAAARLCYSPGSASELVSELKPEETD
                     RLLKHLSRSGHFSPFEHASFVFGVDGISRACSHQLVRHRIASFSQQSQRYVRAEKAEV
                     VTPQTVETNSEALPFFREAIRMAYAAYGELLARGVPPEDARYVLPNACVTHLVVTMNA
                     RELLHFFSLRLCKRAQWEIREVARQMLCLAREKAPLLFAVAGPGCLTKGGCPEERPCG
                     EPYSSMEDLLEKP"
     gene            complement(44812..45048)
                     /gene="rpmE"
                     /locus_tag="PLY83_01155"
     CDS             complement(44812..45048)
                     /gene="rpmE"
                     /locus_tag="PLY83_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOV28675.1"
                     /translation="MKKDIHPRYEECKVVCACGNTFETRSTSKELKVVVCNACHPFYT
                     GKKGRFVSAEAGRLEKFQKKYAGVDYGQRKEEEN"
     gene            complement(45133..45675)
                     /locus_tag="PLY83_01160"
     CDS             complement(45133..45675)
                     /locus_tag="PLY83_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutP family protein"
                     /protein_id="HOV28676.1"
                     /translation="MKLREIFGVKGTSLDGQQAEPIQEDSGRQENSMRGFELPVRLKF
                     TEESQVGRAALLLSCTTDKKSEEELKALLGEEGWKSVATEVGGVASELPQKITRALVG
                     AALNAGVVDKKPREMHALMHAALEAINGFVSLGTLEASIGAKLAIVRNRNWIAVSMVG
                     DTAYHAVAHHERCGLGVMHI"
BASE COUNT         9426 a        14355 c        12002 g        10016 t
ORIGIN      
        1 atccccatcg ccaggacgaa gacgagaatc gtgcttccca tgtccggctg gaggatgagc
       61 ggtacggcgg aaatgcccca caaaatgccg actttgacca gggctccgat tcgtgtttct
      121 tgatccctgg attcgaagag ctttcggctg gcgtggatcg tgaagccaag aaagaggata
      181 tcggccgcct gaaccgtgac caccccaaga ttgacccagc gccttgcccc accggctgcc
      241 actcctgtga ccgggaaaag gggcaagaag cagaaggcga cggcagcaag ccagaaaaaa
      301 ccgctccatt ttctccacgc ttgcagagga atcgaatagg aaccaagcat ggcgatcatg
      361 ccgacaagga tccagaccgc ctgtttcatc cccagcgcct gcgggttgcc aaatcgcgcc
      421 atcgcgatcg ggctggagga agaactgatc atgatgaccc ccatcccgct caggaggagc
      481 gggatcagcc acaagagagg atcggccacg ccctcgtccc ggacgagggc ggtgcgggga
      541 tcagttccct tccgcaggtt caccaagccc ttctccctcc cggatcccaa gaaccagccg
      601 ctgaaagtgc ttgcctctct ccttgtagtt tgggtaggca tcccagctcg tgcaagccgg
      661 agagagcagc accgtttccc cagccagggc ctcccggtaa gccgttgcga ccgcctcttc
      721 catgtcacgg accttccgga tcccttcaaa accggcatct ttcagggcgt cacagatctt
      781 ttcgcgttcg gttcccataa ccagggcaag cctcgcatgt cgcttgaccg cctgggcgag
      841 cggggtataa tcctctcctt tcccctgtcc gccgaggatg acgagcttga ccccgggaag
      901 ggacccgaga gccgttacag aagccgcaac gttggttcct ttcgaatcgt ctacataacg
      961 gacaccgttg attgtggcaa ccgcttcgca ccggtgaggc aaagccgaaa aacttccaag
     1021 aaggggttcg gcagtgcttg catcgaagcc cagggcctcg agtgcagcca tcgccatggc
     1081 tgcgttctca aggttgtgct ttcccagcaa cgggaggctg tcaaaggtga aaaggcgctt
     1141 ggggcctttt tccgtccaga ggaccgcctc cctttccccc atcacgatcc ttccttctgt
     1201 agggaactct tcgtttgagc cgagggaaat cttccgaacg gcatcgaatt cctggaagaa
     1261 atggagatct cgaccctgca ggatcgccca gcttcccgcc ttctgcatcc gaagcaagtt
     1321 cgcctttgcc gcgacatagt tttcgtagga accgtgccag tcgatgtgat cggggtcaag
     1381 attcgtcacg accgcgacat ccacgggaag ccggttggcc cagaacagct ggaagctgct
     1441 caattccatc acgaggacat ccaggggaag atcggcgtaa tccgccagcg gacttccgat
     1501 attccccact gcagccgcct tcatccccat tctctggagc aaatggccta gaagcgcggt
     1561 cgtcgttgac ttcccgttgc tgccggtgac ccccacccgc ttccccttga ggtagggagc
     1621 gacaaaatcc agttccccca ccagcgggat tccccttttt ctggcttcct ggacgggaac
     1681 ggcactaggg gcgactcccg agctgaggac gatcaggtct gactcccaaa gccttggacc
     1741 atgctcgccg aactcaaagg cgatccccat cgattccagc tgctttgcgg cagtttcccg
     1801 aaaagccccg ctgtcggaaa caaaaacccg ggctttcttc cgtttcgcca gagaggccag
     1861 cgaaaggccg ctaacccctg agcccacgat gccgattcgg atcccttcaa tggacgcatt
     1921 cttgttctgc atctgattac ccccaggctt ccatggcctt ctccaagaag aaccccgtcg
     1981 caatcattcc cgcagcatga acaagccaga atcgggtgac cacctgcgtc tcatgccacc
     2041 ccaaaagctc gaaatgatga tggagcgggc tcatcctgaa tatctttttc ccgaaccccc
     2101 gtatggcgac aatttgaatc gcaacgctgg cgatctcgac tccgaagagg aaaccgaagg
     2161 ggaccagcag catcagaccg ccgcctgaaa cacacaggct gaccagaaat cccccgagaa
     2221 agtgggatcc gcaatctccc atgaacaccc tcgccggatg tgcattgtgc cataaaaacc
     2281 cagcacagat ccccgctccc acaaaagagg cggtttcgaa cgccccaggc ccgatgagag
     2341 gaagaagggc cagtcccgcg aagctgagcc ccgaagcccc ggcggcgagt ccatccagcc
     2401 cgtcggttac atttacggca ttcatcattc caacgctgca aaagcccaaa agcgcgatag
     2461 cgggaagcag gccaagggaa attccaggaa acagttccac ccggttcacg aggaggacgt
     2521 aggcactcca cgggagagcg acaagaatct gtagggcgag cttttccagc gacttcaggc
     2581 cctcgctcga acgtttggaa aacttgagcc agtcatctgt gaaccccacg gcaaaggctc
     2641 ccagcggcag ccccaacagg cctgggactg cctgccagga aaggttcccg aacgcgacga
     2701 gacagaggca gaagacgatc gggaaaacga cccctcccat cgtcggggtt ccgctttttc
     2761 tctccaggtg agtcgaaaga ccgtacgact tctccacctg ccggacctcc cacctgcgaa
     2821 ccaggcggat ccactgccct tcgagaatcc ccgtgaaaaa agccgccatt gcggcaaggc
     2881 tccatccggc aaccgtgttc atgggcatga cagcctttcc acgacttgtt caagcccgta
     2941 gcttctcgaa cccttgacca ggacggtatc tttcttcccg accaaacctt ccagaaggtc
     3001 ggctgcctct tttgcattcc tgacccgcca ctgctgaact ttcgccccgg ctggcggttc
     3061 ggtgatgttt tcccactctt ccccgacaag aaccactcca tcaaggaagt cgaacattcc
     3121 gacaatctcg gaatgcagct tcccggattt ctccccgagc tccctcattc ctccgagcac
     3181 ggccagcctc cgcccgggga cggcaagcga cctgagaacc tcgagggctg ccgccaccga
     3241 ggacggattc gcgttgtaag attcgtcaat gagcaggtgc acacggccgt cgcggatcca
     3301 ttttccgcgg ccccgaaggg gagccatccc ggaaatcgct ttcaaaagga tctccatggg
     3361 aacacccagg gccaatccca ccatgacggc aaatccgatg ttggtggcgt gatgagtgcc
     3421 tttgagcagg gatcggcaaa cccaactctg tcccccgtag gacaacccga cttgaaggtt
     3481 cccctcctca aaaacggtat ccgcccccgt taaaagcagg gcgcttccct tgcggtagcc
     3541 gactcctgca attcgaaacg ggcgctgcgc gccttcgatt gcctggctga gcctcgagtc
     3601 gtccgcattg tagatgagca gatccagtcc ttcctgtggg atgatttcca gttttgcaga
     3661 aaggaccccc tccaggtccc ccaacccttc gaggtgcacc ggggcaattg cggtgatcac
     3721 ggcaatggtg ggggggaaat gctccacgat gctgcggatt tctccggggt ggttcgtccc
     3781 catctccagc acgagcactt ccgtgccggg aggcatggcg agaacggtca gggcacagcc
     3841 gatcctcgtg ttgtggcttc ttttcgcaga atggacccga aagaccggtt caagggcgga
     3901 tcggatcagt tcacgggtcg tcgtcttccc cacacttcct gtcaccgcga tcttcacctt
     3961 tggggaaacc tcctggagat acccttctgc gatctgcacc agggcctttt cggcatcttc
     4021 cacgaccagg caggaagctc cggcctccag gatcctgggt tcccacgggg caatcccctc
     4081 ggccgttcca ataacgagtc cggcaccatt gtcgagggcg gcccccacga agcggtgacc
     4141 gtccgcgtgt actccaggaa gcgcgacaaa tccatctcca gggcaaacat cccggctgtc
     4201 cggccaaagg tttccaggaa acgggatttc cggtcccttc acgatcccgc cgcaaaacgc
     4261 agccgcttcc gagaggcgaa gcctttccct atgcatgggg gaactccaat cctcgggcct
     4321 ttgcccaatt catcaccgtt tccttgtcgc tgtagggcaa ggtttgatcc ttgatgatga
     4381 tgtacggttc gggacctttt cccgaaacaa ggacgacatc tcccggccgg gcgtgatcga
     4441 gggcgtagtg aatcgcttcc ggccggtcga ggataatccg gtgcgttttc agcctcaggc
     4501 cttctttcag cccaccctcg atttgccggg cgatttcccc cggatcctcg ctacgggggt
     4561 tgtccatcgt gataacgagc tcgtctgcca attccgaggc gacctccccc atcaaggggc
     4621 gatttggggc gtaacggtct cctcccattc cgaacacgga ccagatcttc ccccgaacca
     4681 catccccgag agttttcagg acgttgcgaa gggcgtccgg actatgggcg taatcgatga
     4741 tgcagcacac accgttttca aaaaggtatc gttccaggcg cccgggaact tgcggaaccc
     4801 tctccagccc caggcggatg gtttcttcgt gcaacccaag cccgaaagcg atcgtcgtgg
     4861 cagtaaggat gttgtaaatg ttgtactgcc cggtcagcgg aaccttccag tcgcgtgcaa
     4921 cgtttccgga cgggaagtgc acatcgagcc gaatcccttc taggtccatc gaccggaccg
     4981 tggcatgaac ggtttgcctg ccgcaacgga ccagcccata gggaatgacg ttgtcaggaa
     5041 actcctctgt cagccttctc ccgtagggat cgtcctcatt cgcggccccg gtccattgcc
     5101 cccgcgtata ttctccaaag agcattcgct ttgcctggaa ataggtttcc atttccccgt
     5161 ggtagtccag gtgttcggga gtcaggttcg tgaatccgac cgcatcgaac tggcagcctt
     5221 caagacgccc ctgaaccagc ccgtgggagg aggcttccat cacgcaggcc tggcttccgt
     5281 tttgcaccat ccgcgaaagc cattcctgaa gctcgacgca atccggcgta gttcgttcag
     5341 caagggtttc ccttgtgccg tcatggtaaa caatcgttcc tagaagtcct gtctggattc
     5401 ccgccgattc caggatcgaa cggacaaggt aggtggtcgt gctcttgccg ttggttcccg
     5461 tgatggcgat cattttcagc ttcttgccgg ggtatccgta aaagtgcgcg gcaagggagt
     5521 tgaccgcctt tctcgcgtca tcgacgagga tctgcgggat gtccacggca agaggcctct
     5581 cgcagaggat cgcgacggcc cctttgagac gggcttcttc ggcataatca tgaccatcgt
     5641 gccgtccccc tggcaggcag cagaaaaggg atcccggagt cactttcgcg gtatgatgag
     5701 cgattctttc aatctcaagc tcgcttgccg gggaaaccgc cccccagact gcagagcaga
     5761 aatggccttc ctcatcaaga tgggcaatga ttttcctgaa atccatcggt tcacgactcc
     5821 cctccgggcg catgatctat cgggcgagca aaaggtaatc ctcaagaatc gccctgaaag
     5881 caggcgcagc aatcgctccg ccgaggtgcc ccttctgcga tggttccccg aggacgacca
     5941 gcaggatatg gtctggattt tccgcgggcc agaacccgac aaaggaggca acgaaccgcc
     6001 cttttacgta tttgccccgc tcagccacct gtgcggttcc ggttttgccg cccaccgtca
     6061 cgtgagggga attcgcggcc ttgccagtcc cattcgtgac gacgcggcgc atcgccgatc
     6121 gaacccaatc gctggttttc ttggagatga ccacgttcct gacgcagacc tctccccggt
     6181 agaccacatt tcccgtcccg tccctggctt cctttacgat gaacggccgg acgcaccgcc
     6241 ccccgttgat cacggcggag agggctgcaa ccagctggag aggggttacg ccaatcccct
     6301 gtccgatcgc gatattggct gggacaacgc ctctccattg ttccggggtg ctgaccagtc
     6361 ccgcctcctc acccgggagt tcaatcccgc tcggatcgga aaacccccac tgtctcaggt
     6421 tgtcgtaggc aaatctcgga cggaaacgga tcccgatctg cgccatcccc acgttgcagc
     6481 tattgacgat cagatcttgc aggatctcgc tcccgtgcgc cttgatgtcc gtcaccgttc
     6541 catcggcgat tcggattcgc ccggcgcagt gaaaggcctc gttttccctt accaggccct
     6601 gctccatggc agtcgccata atgaccggct tcagggtgga accgggctca taaacccttc
     6661 cgatgacgtt gttcaacctg actgccgggt cgagcagcgt ttctctccga ttcgggtcga
     6721 aagtcggcca ggaagcggag gcaaggacag ccccgcttct cggatccatg cagaccgccg
     6781 cggcccattt tggcctgtat tccacggtcg cctcctcgag atgcttttca acgaccatct
     6841 gaaggatcgg atcgattgtc agggtcaact ctcctggtcc atgttgcttc ggggctcttt
     6901 ggagcgacaa aagatcgatg ccctgccccg ccgcgtctct tgccaggatc ttcacctggg
     6961 gcggcgagta aagaatctcc tgccaggact gttcaagtcc ggccagcccc tcgtcgtcga
     7021 tatcgcagaa accgagaacg tgagccagca gtttcccctg cgggtaaacc cgtttccttt
     7081 ccttgagagc gtagatccct tccaacttct tcgaaaggat gcgggcagcc acctcaggcg
     7141 gaattttcct ggcaacccac acaaaacggc ctttcatggg cctggagatc ttttcaagca
     7201 cttcggggga aatcaggtct cccagaacgt ttttgaccac ggcagcattg gcgggatccc
     7261 agtattgtgg gtcgatgaaa aggctggttg cgggaatgga aagcgcaagg gcggaacccc
     7321 ggctgtcgcg gatcactccc cggcttgtac tgatggcgac ctgggtccag tactggctgt
     7381 tcgcttgcag gacggttctc ggatcaggcc aggcctgaac gaacacaagc cgtgccgcca
     7441 aaagaccaag ggcaagaaag agccaggtcc agacacttcc aaaccgtcta aacaggctta
     7501 ttccgactac ccccttggcg actggatgaa aaaaacgaga ttaatcctgc gcgtgagctt
     7561 ctttctggaa caaaccggca aggctgaaac cggcaacagg cgctttctga cctgagattg
     7621 gccgtaacgc aacggactgg gcggggcgag ctgcaggcgg aaccgttacg acggcgactc
     7681 cggcgtccga aaccatgccc atttttgcac tcgccacact atacactctt gatggagata
     7741 gtaaagaagc cagtttgttt ctcaaatcga gttcaagctc ctggtagttt tcaatggtct
     7801 cgttgaggcg ggtcagccgc tgttcgaggc tcatgttgta gatccgcagc cctccgagcc
     7861 ccatcacgag aaccccgaca aagaccgcca cggccgcaaa tgcggaatgc gcccgcccct
     7921 ttcgctgcat ccgttttcga ttcccgaccg gagacggcat cccctttcac ctccctggat
     7981 cgatttcttt tccgttcatc cggaagacgc gcaacatcgc gctcctggac ttgtaattgc
     8041 gctggacttc ctccggagag gcctgaattg ccttcctcgg catccgacgt cccaaaccgt
     8101 ccttttccca ctggttgaag gcatgtttta caatcctgtc ctcaagggag tggtaactga
     8161 cgaccaggac acaaccgttt tcgccgatgg accgaaccgc ctggttcaat ccctcacgca
     8221 gggcttccat ctcgtcgttc acagcgatcc ggagggcctg gaaaaccctt ctggccggat
     8281 gccggttcat tttcctctga agcggcgccg gcaaggcgtt tcgaatcacg ttcaccaatt
     8341 ctgccgttgt ccggatagga cccttctgat cccggtgttt cacgatcccc ctggcaatcg
     8401 gtgcggcatg cggttcttcg ccataatccc tgaaaagtct gcgcagttcg gcttcggata
     8461 acttgttgat caattcctcc gccgtcgttt cgcccctacc catccgcatg tcaagcggcc
     8521 cttcctcctg gaaggaaaat cctctttccg acagcgatag ctggaggttg gaaaccccca
     8581 ggtcgaagac cacgccgtcc ggccttcccc aacccgtctc gctgacaagc tgctggagac
     8641 atcggaagtt cccctcgaca acacggaaac ggcctgcagc cagcgggccg gacaaacgtt
     8701 ccctggtccg cttgagggcc atcggatcct gatcgatccc gaggaccctg caagacggcc
     8761 actcgagaag aaatcgctcc gagtagcctc cgagtcccac cgtggcatca acgataaaag
     8821 cggggggaca ttccctggac aattgggtca cgacttcttc gaggaggaca ggaatatgct
     8881 cgatcaaaag ccgtcaacct cctcggcgat ttccggcagg gtttccagaa ccctcgtcct
     8941 gtagcggttc cactgttcgg agtcccaaac ctccagatga tcaagaaccc cgttgatact
     9001 gacctcctgg tcgattcccg cctgctccct gagggattgc ggcaggagaa cacgacctgc
     9061 gggatcgatg ggaagttcat gggcggaggc aagaagcacc cggatcaaat cccgggtttt
     9121 cccctttgaa tagggcaggt tatgaagttt ttccagaatt ccttcccagc ttttcagcga
     9181 gtagatggac acgcagcggt cgattccgaa agaggcgacg acactgtccc cgagttcctc
     9241 ccggaaccgg gcagggatca ctaggcgtcc ttttgagtcc agcctgtgtt caaatgtccc
     9301 caccagcatc gaaaaacctc ccacaaaatg acactttatc ccacttatgc ccactttatc
     9361 acttcacacc cgctggatat gattctattg aatcaaataa accaattgat tatgggaggc
     9421 aggtcctatt tttagcgttc gttcatttgg ctggccaagg attttcgacc tggaagaaga
     9481 gcagaggaca aaaaaagccc cccttaaagg ggggccgggg agtatcccat aagccgggtt
     9541 ctgtcaagaa cgaccattta tctagacttg gacttgcgcc caagttcaag cgaccgtacc
     9601 cgggggtcag cgggccgcct catccccccc tattcggtct tgctccggac ggggtttgcc
     9661 tagcctgcag gtcaccctgc agctggtggg ctcttgcccc accttttcac ccttgccggc
     9721 agcgatgccg gcggactgtt tctgtggcac tttcctgagg ttcgcaccta ctggacgtta
     9781 tccagcgtcc tgccctttgg agcccggact ttcctctccc gccggtggaa acaaccggcg
     9841 gcagcggtcg tttgtgatac tccccggatc cattttaccc cagaaccgcg cttgacggcc
     9901 tatttcaggg aagcgtgcaa aaacgcgagt tccacgtagg acgctgccag ttctcggatc
     9961 cggtcccgtt cttctggccg aatcgaacgg accacccttg cgggaacccc catgaccagg
    10021 cttccgggag ggaccttttg tccctccggt acaaggcttc ccgccgctac gacagagccc
    10081 tccccaatct gggccccatc gagcaggatc gcccccatcc cgaccaggca ctcccgatca
    10141 acgacgcatc cgtgaaggat tgcgccgtgt ccaacggtca cgtcgggacc aatcctgacg
    10201 ccccgttcga gagtgacgtg gaggaccgcc ccatcctgga tgttggctcg ggatccgatg
    10261 cgaatgctgt tcaggtcccc tcgcaggacg gatccgtacc agacgctcga gtccttttcc
    10321 aagaaaaccg agcccacgag aacggcggag gcggcaacaa aggccgcttc cgccgtttcc
    10381 agcttccatt cgcaggggaa tttcctcttg tccatgatcc tctgccccta atgggaccgg
    10441 acgatttcct tgatcaccat cagccggctt tggctggaac gttccatctc atccagtttc
    10501 atcgtgatgt cccggatcgt ttccttgaat gacgggatca tgacgtgttc gagagcgttc
    10561 acccgccgcc gagttcgctc gatttcagaa gccatgagct ttagggcttt ctcatcggcc
    10621 gcaagcttga tcagcctgga gatcagccgc gaaaacttct ccagcgcgat atccaggctt
    10681 ccgggagagg tcgcaaggcc gtagctgagg gttcccccgc cctggttcac ggtgtattcg
    10741 ggaacgatga cgctcatgac gttcctgtag tcgacttcca ggctgcagga agctcccgaa
    10801 atcatcagag actgctccag catggccggc aacgtttgcg ccctcgcaag gaggaagctg
    10861 cggtaacact ctgccagctc gccctccagc tgttcccgca agagtttgat cgcccgcgcc
    10921 cgcccaagga attccttgat gagggcatcc tgtttgtcct tcagaagctt gtgtcccctc
    10981 ttcgccacca ccaggcgctt tttcagcctg gacagctcca tgcggttcgg gttcacattc
    11041 agtcgcttcg ccatgggtat gcccccttaa gacagaacgg cttccgactc gttggcctcc
    11101 cgttccttca ggagcggctt gagatacttc tcaacgtagg catcccgcac gcgcttcagc
    11161 tcccgcacgg ggatcatcgt cagcaattcc caaccgaggc ccagggtttc ttcgaccgtc
    11221 ctgttttcat attcgccctg cctgacataa cggtcttcga aggcatccga aaagcgggcg
    11281 aacgccttgt cttcctccgt cagagcccct tccccgagga tgatcgcgag ttctttcgct
    11341 tccttgcccc gggcgtatgc agcaaagagc tggttcatga ggtccgcgtg gtcttcccgg
    11401 gtctttccct tgccgatccc cttgtctttc aaacgggaaa gcgagggcat gacatccacg
    11461 ggcgggtaga tcccctttcg gtgcagaccc cggctgagaa tgatctgtcc ctcggtgatg
    11521 tagcctgtaa ggtccgggat cggatgcgtc ttgtcgtctt ctggcatcgt caggatgggc
    11581 agctgcgtga tcgatccctt tcttcccctg agccgcccgg cacgctcgta catggtcgcc
    11641 aggtccgtgt acaggtaacc ggggtaccct cggcgcccgg ggacttcttt ccgggccgcg
    11701 gaaatctccc ggagggcctc acagtagttg gtcaagtccg tcaggataac caggacatgc
    11761 atgtcgcact cgaaggccag gtattccgcg gcggtcaggg ccaaacgggg cgtcgtgatt
    11821 cgttcgattg ccggatcgtc cgcgaggttc acgaacatga ccgtccgttc gattgcaccg
    11881 gttttccgga agtcttccat gaagaacgag gcctcttcaa aagtgatccc catggcggca
    11941 aagacgacgg caaaggcttc gtgcccgctg atgaccgccg cctggcgagc gatctgcgcc
    12001 gccatccggt tatgagggag accgcttccg gaaaaaatcg ggagcttctg gccccgaacc
    12061 agagggttca tcccgtcgat ggttgaaatc ccggtctgga taaattctga cgggaaatcc
    12121 cgggaatagg gattgatcgg gttcccgttg atgtccagca gggcctcggg aatcactccg
    12181 gtccctccgt cgaggggctc tccacggccg ttgaagattc gtcccagcat tgtccggctt
    12241 accggcaggg tcaggacctt tccgaggaaa cgcaccttgg tcgattcgca gtcgatgccc
    12301 gtggtccctt cgaaaacctg gaccaaagcc ttgtccgtcg tgatttccag gacgcggccc
    12361 cttctcctct ctccggaagc cagttcgatt tccacaagtt cgtcgtagcg gacctcgttg
    12421 actttctcga caaccatcag cgggcccgaa aggttggtga tggttcggta ctccctaggc
    12481 aacatggacc tcacccccag ccggcatcag attgccgatt tccttcttga tgtcctcttc
    12541 aagctgatcc agcaactgca gttccttttc gtcaacgtag cgcatacggg taatgcgttc
    12601 ccggatcgcc atatcggtga tgtccttcag ggctgcaccc cgctttagtg cctccatcgc
    12661 cagatggtgg aacgtcagga tgttcttcag catcttgaac tgcttgtcca tcgaagcgta
    12721 ggtgtctact tcgtggaaag cgttctggtg aaggaagtct tcgcgcaggg atttcgccgt
    12781 ttcgaggatc atccgctcct ctttggagag ggaatcgatt cccacaaggc gaacgatttc
    12841 cttcagccga tcctcgtcct ccagcaacga catggcttcg atgcgatttt tggcccactc
    12901 cccgtcataa attccatccc agtactcccc aagtttttcg gcgtaaagag aatagctgct
    12961 cagccagttg atcgcgggga agtgcctctg gtaggccagg ctagcatcca ggccccagaa
    13021 aacctttgtg acgcggaggg tattctgggt cactggctcg gacaggtcac ctccgggagg
    13081 cgaaacggcc ccgatgacgc tgacggctcc ctcaaccccg gcctttccaa ggcagattgc
    13141 cctgcccgcc cgttcataga acgaggccag tcgggtcccc aggtaggcag ggtacccttc
    13201 ttcgcccggc atttcttcaa gtcggccgga aatctcccgg agggcttccg cccaccggct
    13261 cgtcgaatcc gccatcaggg cgaccgaata ccccatgtca cggtaatatt ccgcgatcgt
    13321 gatccccgta tagatcgaag cctcccgggc agcgacgggc atgttcgagg tgttggcaat
    13381 gagaaccgtc cgtttcatca gcggttgacc ggatcgcggg tcttcgagct cggggaattc
    13441 cagcagaaca tccgtcatct cgtttccccg ctctccgcac ccgatgtaga ccacgatctc
    13501 ggcatcggcc catttcgcca gctgatgctg gatgaccgtc ttcccggacc cgaacggtcc
    13561 cggaacgcag gcggtcccgc cccgggcgat caggaagaac atgtccacga cacgctggcc
    13621 ggtcgtgaga ggcaccaccg ggggtagacg ccgggcaaca ggacgggggg tccgaaccgg
    13681 ccagcgctga agcatctgga cctcgcggcg ttcttcgccg tcgatgagaa ccgcaatggt
    13741 ttcttcaacg gtaaaatccc cttcccggat ctcctcgacc ctcccctgca ttcccggcgg
    13801 aaccataatc cggtgctcga ccagaacggt ttcctggaca accccgagga catcacccga
    13861 aaccacgaag tcacccttct tcactttagc ccggaaggtc cacttccggg cgcggtcgat
    13921 cgccggaacg ttgattccgc gtgaaataaa ggggctctgg gccttttcct cgatcgcgtt
    13981 caaaggacgc tgaacgccgt cataaaactg ctcgatcatt ccggggccga gttccacgct
    14041 caacggtgcg cccgtactga tgaccggctc tccgggcatc aaccccgagg tttcttcata
    14101 aacctgaacc gaagccaggt ctccctttag ttcgatgatc tctccgacta ggccgaggtt
    14161 tccgacacga accacgtcgt acatgccggc cccggtcatt ccgctagcaa cgacaagcgg
    14221 tccggagatt ttgctaatct ttccctttac agacttccca acggccacat ggatcgcctc
    14281 caacctgttg catcgttttt cgatcagccg gtccaatccc gatttacttc accgaaaaga
    14341 tgtccatccc gactgcccgt tcgacactcg acttgatcga tttgaaaccc aagcccctcg
    14401 atccccggag tccaggaatc ggaacgatgc tcgtgtcttc cttttcgttg atttcctcga
    14461 tccgttcctt gaggcgctcg taccagtcct cgacgacaaa aacgacggcg tatttcccct
    14521 gaacaagctc gttcaggatc cttttcgcct ctgcatccgc cccgtcttcc agaacgtgcg
    14581 gttcaatccc caccgcctga aagggaagta cgctctcgta atcgccgaga gccgccatcg
    14641 acctgtttcg gctaaccgct gacatgccgc agcaaccccc tcaggacgtc cctgttggct
    14701 ccgttcgcct tgccgacaag gatgatccgg acattcttcg cttccatttc ctttagccag
    14761 aggtagtaaa ggacatactc cggagcagtg gaactgtatc gagtcttctc gacaaccctg
    14821 aggacgtaat cgtccagtgc tttttcaacg tcgacgatga gtcggtcgaa atcggagatc
    14881 tcctgaagct gtgatagggc tccggtcagg tcggtgtagg ccagcaatct tccccagctt
    14941 tccagaggtt cgctcgtcag ggagaggagt cgctcaacgg taatgatgcc cccctcgtgc
    15001 aggaatccgg caatccggtt gttctcgacc tgcatccggc ggagcctcag gaggtttctc
    15061 acgttttccg cgtcgatcct gccccggacc cattccacga tacctttccg ctcaagtttt
    15121 gcggccagct tcagcatttc ggagaaaagc agatcgtcaa gctggcgttc gatctcgagg
    15181 atgtcatgga actgttccca ctgggcaaga gactggggga cggcactcct cagtccgaaa
    15241 ggcaaaaggc ggtaatcctc cgactcgagc gattcaatca aaaactcggg ggaaacgttc
    15301 cccagccggg tcaggagatc ccagcgcctg gggccgccgg atttctgcaa aaaagagctc
    15361 ttcaacagca ccttgacgtt atggaagtca tacggcatct tgcagagggt gaccagcgcc
    15421 ttgtcaggga caaattgtgc ggtttccctg tacacgtagt tcagctccgc ctcgatggcc
    15481 ccgtcgaaag cgccctcgtt cttcatctcg acgagccagg aagaatagca ggtctcattg
    15541 agaactttca atgctccctg caggtcctcg gcatccagca tcctctgaaa ggcaccggaa
    15601 tccaggaggc gattctccat cgccctcagg cgtgcaaccg catagccata actttccgcc
    15661 tgggccattc cctcacctcc cgtcaattgg aaaaaagttt cttgaccgtc tccgcctcga
    15721 gctcttccct ggccgtccgg atcagcattt cccaggaaca gttcacgtcc accaggccac
    15781 gccggagaat gaatcctccg gtgatcggag ccttttcctc ggccatcttc aggttccagc
    15841 cgtgtttttc attgcattgc tgcaaccacg tggcatccag ggctttttcg tcccgtccga
    15901 caaggagcca ctcctcgcgg gtttcaactg ccttcaaaag cagggtttct gcaaagcgcc
    15961 tggcctcatc ggccttcatt ccgcacatga gcccttttgc cgcttcaaag gcctcgtcga
    16021 tcagggttct tcgggcggtc aggtcctggt tgttcacgtc cagcttcgcc acgatttcct
    16081 tcctgcgcag gatttccggg atttccgctt ccaggcgctt tttctgggtt tcctccatcc
    16141 gtcggatctc ttcctcggtt tgacggtgga tcttctcagc ctggctccgg gcggaagaaa
    16201 ggatctcttc cgcctcagcc cgggcatcct cctcgatctt cgccttgata tcggtcagcg
    16261 acatgccaga caccccggct agagcttgat gctattcatc aggatgatac taagaaggag
    16321 cgcgagaacc gcgtaggttt caaccatggc gggaaggata acggctttac cgatctcttc
    16381 cggacgcttg gaaatcagca ggatggcacc agcggaggct ttgccctggg cgaccgcgga
    16441 ataatagcat gcgatcgcga tgggcatcag ggaaccgaaa attccaatcc cctgccagat
    16501 ggtcactttc acggcggctc ctccaagcaa acccactttc aggatggcaa aaaaggccgc
    16561 gaggaggccg tagatcccct gggttccggg aagcgccagc agaagcaggg cgaggccaaa
    16621 cttgcccggg tcttcagtca tgactcctgc tgccgcctcg tccgcgatcg cgattccgat
    16681 cgcggagccc gaaccggcga accccgctgc aattcccgcc ccgaggacgg caatcattgc
    16741 cccaagctgt tccgacatgg tattcaagag agttgcgaca acctggtcca taagttccat
    16801 tcctccttag taaaaatggg gttttcttca atcaatcact gtttttcctg ctgtccgcag
    16861 gaatcaacga tcttcacgaa ctgcgtttcg tggcaaaaag gagaaaagag ccttccgttc
    16921 gcctcgtaga acttgctgaa gaactcgacg tactggaggc gcagggaatg gacgaaagcc
    16981 cccaagacgt tgatcgccag gctgaagatg ttgccgacga caaaaaggat cagggcaagg
    17041 atccagccta cgtacggaac tccctcggag atcatcttgg caagcaggtt gataatcagc
    17101 ccgatggcgg cgcctgatag ccccagagcc agaagacggc tgtaactgag ggtatcccca
    17161 aggtaggaag tgatcccgta caggctgagc gctcccgaaa aagctttccc aaggattccc
    17221 ttcttgctcc tcccctgggt caataacagg gtgaccgctc cagcgatgga caggcttttc
    17281 gcaagcattc ccatcgaggc gggggctttc ccgatcatga cgaggaaaaa gagggccaca
    17341 ccgttaagca gaaggatcca gcctccgcgg tcggcaaagg cctccaggta ggattttcgc
    17401 cggagggcat cccagaaggc aatgcaaagt ccgaaaatca gctggacgaa cccaagggcg
    17461 aggcaaactc cgaggtaggt catcggatcg ttcatcggat cccagacaac caaacggtcc
    17521 ctgagcggac gaaggaaatg gaggaaagca aagctgtcga tcgtgttggc gagccacgat
    17581 cccgtaaggg cgcccatgac gatcgtcgaa aggccgcaaa tgaacatgac gacgaggagt
    17641 ttcctgaggt tgccgccaac ggggaacttg aggagaaccc acccaagcag ggccgacagg
    17701 atgatcccaa agcccgcgtc gccgagacac atgccgaaaa agacgtagaa aaagggggcc
    17761 atcatgagcg tcgggtcgac gcccccgtac gtcggaacac cgaacatgtt cgtgaggggt
    17821 tcgcagggtg aagcccaggc tgggtttttg agcagggagg gagccacttc cccctcttcg
    17881 ggagccttga ccgtcgtttc ggaaagagaa ctgaacggtt ccagttcttt tttgatcttt
    17941 tccagcgact ctgcaggggc ccagaaacga atgaaatggc tttgttctgt cctttcgctc
    18001 gaatcaaggg cctcgtaacg ccccttcatc acgctccaat aatccgacag cgcctgtaca
    18061 cggctcagcc attccgcggc aagcgatctc gcttggtttt gcaggtcggt ttccctcttc
    18121 accaggagct ctttccgtgc ttccagccga gcgatttcct ccccgaccgt tccggaaaga
    18181 tcccgtggaa gatcgatccg gttgaaagag acttccgagc agatggaggc caccttttct
    18241 tcgttctcgc gaaggtaaac gagagctgct ttcgcgtcct tatccttttc cgggttgttg
    18301 attcggacaa acagctcccc ctcttttccc acgtgagcgg aaaaggcaac ctcaaggcga
    18361 tcgacctggt ctgccggtaa cgtgccaagg atccccttga ccctttccgt tccggttgtg
    18421 aaaaagaaca gaggctgggg aagttcgccg aaggctcgca gaatcccttc caatcctgcg
    18481 atctgtgaga gttccgttcg aatttctaca aactgtcttt ccaggttgcg aaggtgaagg
    18541 atgagttgct gaaaatccgt ttcctttgac caggattcca gttcgctcat cgaatgctgg
    18601 ggtttttcag ccagggccct agagagaaaa ccggcctttt ccccccggta ggtttccagg
    18661 aaacgcaagg cgaagcgcgc atcggccagc aggttgtcat agcgcttgag ctcatccgat
    18721 ggcgtaaccc ccccggcctt ctcagaagcg ctgtcgggac ctcccctgat gatctcgcaa
    18781 caaccgaggc gctgcaggga cgcgatcacc cggtccgtca cggatcgatg cagggcaagc
    18841 tcgacctttt tcatctcaac taaggccata tttcgccgtc acctctttca ccagccaggt
    18901 cgccacttct tccaccctcg acttgcgtgc ctggacgaag gcttccgtct gcttcttccc
    18961 ttcggcccgg atcgtttcgg cgcgcttctc cgcttgcgct tccgcctcgg cgatttcctg
    19021 gcggaacttc tgatgcagtt cctgcttcgc ttccttagcc cttctttcac tttcggaacg
    19081 cgcatcggaa acaagacgag aggcttccgc ttttgcctgc tgtatgattt tttttgcgtt
    19141 ttcctctgcc tgccttattt cttcagccaa acttgtcgcc attgctttca cctcccttgc
    19201 aaaccgtttt aaaaaaatgc attctacaat acacagaaaa attcaccccg agtattctaa
    19261 tcggcaacgg aatattgtgt caattgtcac aatcggcggg aaaaaaagga aatttgttcg
    19321 attcaatcaa aaaagggagg ctttctccgc ctccctccga attcctatgg agcggacaac
    19381 gggattcgaa cccgcgaccc tcggcttggg aagccgatgc tctaccagct gagctatgtc
    19441 cgcatctgcg ggggatatta tatcgacgag gtccttcttt ggcaaggcaa atttttcgaa
    19501 atcgcccgga attcccgaac cgcgctgcag gactgctgaa aattccctat tgaagccctt
    19561 cacagggaaa gatgcgaaac gtttctcaat ttccgagaag gccaggcttg tcattacgtt
    19621 ttaaatggat tattatgtaa aaacagttat tgttctggag ggttgtcaaa tgtggacact
    19681 tgacgatggc atcgccaggt taaaggaatc ggggacaaag atcacggcgc agcggatcgc
    19741 catcctgaaa cgcctggaag gtcgaaggga ccatccttcc gccgagcaga tctaccatga
    19801 actcaaaaga gattttccga ccatttcctt tgccacgatc tacagcacgg cgcagcttct
    19861 ggcagaaaaa aacctcgtcc agattctaac gatcgacgac aagcgggtca actatgaccc
    19921 caatcccgag ccacacggtc acttccgctg tacggcctgt gggcatatcg aggacgtccc
    19981 gatcacggaa tcgttgcttg aaatgctgaa gatgggacag gattcaacga tttcaagcgt
    20041 ccagttgtac atgtacggat tgtgccccag ctgcaacgtt cggtaagtgc tcttcgacag
    20101 caatgatcct ctttttttcg ggagcggtta cgctccttgt ccttgcaatt gcgctgggcc
    20161 ttcaggcagg ccggcttaaa agcaggaaag tgagaagact tttctggctt tccctccttt
    20221 gcgggctttt tgtttctgcc cttttccttg ggtgggccgg aagatggtgg ctagctgccg
    20281 gtccacttct cccttttttt ctgattctcc tgctttggat tcgtttccct ttcatttttg
    20341 acggggcagc cccccctcaa aagcaggccg aaacgaaaac ggaggaagat cccaaaccgg
    20401 aaaagggtat aattgagtca tgaaaacgga cgctgcccat cacccacgag aactctcacc
    20461 tcttcaaaag ctcctggttg gccttgttct cctctacggg ttggttttca ccccagcctc
    20521 cgcacaggcg gcaacaccgc agcttcttta ttcgatcctc cttcctttcc aggtcggcgg
    20581 aaccgtgaaa gtcatccttc cggacggatc agaacaaccg atcggtacag tcgagatgct
    20641 cccggagaaa actcgatggc ccgcctacac ggcaacccgt tggggaactc cgggaaccgt
    20701 tgccgcttct gccgtcaatg caattcacct tttgatcgac atcgaaaagg gccgaggcag
    20761 gactctcagc ctgctcccct ccgaaaccat cgcccctgca gccggaccgg ggagtgccct
    20821 cgtcgtcacg gggaagggag ggtacggact tttcggaggc tgggcaccac cggtgggttc
    20881 gccggtgacc gtgatttcgg cttccgggga agaacgcccg ctgaatgggg gatcgatccc
    20941 tcgggaggga gaaaccctcc ggatcgaagt gaacaacctc tgctccccct atatgatcga
    21001 gattgaaaac cggcccggcg gtagggtgtt tgcctggtcc cgtgccgcgg aacaaagcgg
    21061 tgtgatcgcc cgggtcatcc ggcccgttcg gggagtcggc cgttttgaag gaaccctttt
    21121 ccaatccgtg ggacggctca gggcgaacca ccccggcgtc atcgacatca gcacatcgcc
    21181 cgaaggggca atcggtggtt tccagatcat ccccttcaat catgcccatt ccgcggagat
    21241 ggaaggggca tggcaaagga cccaatggct gatcattgat tctcctgacg gcaaaactcc
    21301 gctcaccggg cgccctccgc tcttcggagg aatcctcgtc cccgggcctc gcgagacgga
    21361 acgtctttgg gatctctggt caacctacgg caggagaccg ctcgtccttt gccgcatcga
    21421 cggcggcccc tggacaaggc tgccggaggt gatcggcaaa caggatctgg ccctggagaa
    21481 tgtgacgcat ctccgtctct attttcctgt tgtcgaagag ccgaacctct gaagcgttga
    21541 tcacggaata aaagggatgc gccggacacc gccggcgcat cctttttcgt ctttccctgc
    21601 cgttttctct tcctattcca cggtcacgct tttcgcaagg ttccttggct ggtcgatctc
    21661 gcattccctc agtcttgcca tgtgataggc aaacagctgc aagggaacca ccgtcagaaa
    21721 cggggtcatc tcctcgtccg tccaagggac gcggaggaca tcgttcgcgt ggcgggagat
    21781 cagatcgtct ccatccgttg caaccgccag gatcggagcc ttcctggcct tggcttcctg
    21841 aatgttggaa agggtcttct cgtaaagccc gtccctcggg atgatgacca taaccgggac
    21901 ggcagcctca agaagggcaa tggggccatg cttcatctca ccggccgcgt aggcttccgc
    21961 gtgaacgtag gaaatttcct taagctttaa cgcgccttca agggcaacgg ggaaagacat
    22021 ccctcttccc aaaaagagaa aatcctttcg gtccgcatat ttctctgcaa gtgcacggac
    22081 ggagtcttcc ctttcgagag tgacctcaac cttgtagggc aacctcagca tttcttccag
    22141 aaggcggttt ccctccgcag ccgaaagggt ttctctcaag cgccccaggt aaatggcaaa
    22201 aaggtacaag gcggcaatct gtcccataaa cgtcttggtg gccgccaccc cgatctcggg
    22261 tccagccttc agaagcagga catcgtggac ttcacgggcc agcgtcgatc cacggacatt
    22321 ggtcaccgca acgcagtggg ctccctgtaa tcgagccagg cggcaagctg cgagggtgtc
    22381 tgccgtttcc ccggattgcg atacgaatac ggcgagcgtt tccgtatcgg ccctcaggtg
    22441 gcggtaccgg tactccgaag cgatgtcgac cttcacttca aggtccgtcc atttctcgag
    22501 gactctttcg gcaaccaggg cggcataata ggaggtcccg caggccacga tgtgaagctt
    22561 cctccaggat tgggccagct ccttgtccca ggcaagctcg ggggaaaggt cgactttccc
    22621 gttttccacc ctccccttga gagtcgtccg gagaaccgcc ccctgttcat ggatttcctt
    22681 gagcatatag tgggggtagg ccgccttctc cgtcatggag acttcccagt cgatggtgga
    22741 aatgcttttc tccacccgtt gcccatcact tgcccatacc tgaatgccgt ccctcgaaac
    22801 ggccgcgatt tccccttctt ccaggtaaac cacctggcgg gtatacggca ggagggcagg
    22861 aacatccgac gcgcagaacc cctctccctc cccgatcccc aggatgagcg gggagccttt
    22921 cctgaggcag taaaaggtcc cgtcgacctc ccgattcatg atcgcaagag cataggatcc
    22981 ccgcagtttt tcggtaagtt tcagcaaggc atccagcatg tctttccggt agaggcgcgc
    23041 cagaagctgc acgatgacct cggtgtccgt ttccgaagtg aaggataccc cggccgcttc
    23101 gagttcttcc cgcaaatcca ggtaattctc gatgatcccg ttgtgaacga gcacgaaatt
    23161 tccgcaatcg tcacaatggg ggtgggcatt gtccacggaa acgcccccat gggtggccca
    23221 ccgcgtgtga ccgatcccct ggcagccttc aagctgcctt tcctcgacaa ggcgcgccaa
    23281 atcggccacc ttgccaacct ctttgatcac ttcaatgcgc ccatcgctct tcacggccag
    23341 tcctgcggag tcgtaccccc ggtattccag cctcttcagt ccttccagaa gaacgtccgg
    23401 ggcatttctc atgcccacat agccaacgat tccgcacata ttcctttttc tctcctttgc
    23461 ttcctcaagg tgggagaccc ggcctttgtc ccgcaaatcg cttcgcggtt ttgtagccgg
    23521 gctttcgtgg gcctctctca ccaaggcccc ggggtatccg ccgaattttc gattcgcccc
    23581 gacctcgtca actggcttcg ccagttcagg cgcttccacc ggcacgcaaa cggttcaaaa
    23641 cccttgagtc gttgccttca gtcattgcat tcctccttcc tgacaatgct caggatggct
    23701 tcccggagtt cagggtcatg caacgcaagg gtgaccgtcg ccttcagcca ccctagcttc
    23761 gttccgcagt ccagcctctt tccgcgatac aggaggccgt agacgggttc ttccgccaga
    23821 agagcctgca aggcgtccgt cagctggatc tctccaccga ctcccgcctg gagcgaacga
    23881 aggcacccga aaattgaggg gctgagaaga taccggccga tgatcgcaaa gcggcttggg
    23941 gcctgcccgg gagagggttt ctccaccagg ttcgtgatcc ggaaaacccc ctgtccgcta
    24001 gggtacccct cgatgattcc gtatcgggag gtttcttccc aggggacctc ctcgagagcc
    24061 agaaccgatc ccccgaggga ctcatgaacc tcggccagct gtccgaggac cggttggtcc
    24121 gaaaggatga cgtcatcggg gagaatgagc ccgaaaaaag aatcgttgaa gcagggttcg
    24181 ccgcaaagca cggcatgccc caacccaagc ggctcggact gcctcacgta ggcaaagcgg
    24241 gccatctctg aaatcctggc cagctcgtcc ctgagtccga gttgccccct tctttccagc
    24301 agattctcaa gctcaaccga acggtcaaaa taatcctcga tcgaccgctt gctccttccc
    24361 gtcacgaaaa cgatatccct gcagcctgag gaaaccgctt cctccactcc atagtgaatg
    24421 atggggcgat cgatcagggg aagcatctct ttgggaatat ccttggtagc gggcaaaaac
    24481 cgcgtgccca aaccggcaac gggaaaaagg catttccgaa gcgggtgccg gacgctcatg
    24541 gggttagccc ttcctttccg acgccgattc agcgccagct gcacggatcg tttccaggat
    24601 ccggtccgtg atttccgaaa gaagcgattc ttccctggcc tccacgagga tccggacaag
    24661 cggttccgtc ccggacatcc gaacgaaaac acgccccttg tccccaagtc tcagctggca
    24721 atcccggatc gccttctgaa ctccctcgct ctccagaatc cccgatttcg tttcaacacg
    24781 gaagtttctc aggagttggg gaaaccgtgc aaaacgatcg accagagtat ccagatcttc
    24841 tccaagttct tcgcaggcct tcagaaaatt gagtgccgtg cagagtccgt cccctgtgtg
    24901 gacgtgctgg tacaggataa tatgccctga ctgttctccg cccagccttg aattgctttt
    24961 cttcatttcc tccagcacgt agcggtcgcc aaccgggcac ctcgtcaggg gtatcccggt
    25021 ctccgagaga cgctcctcga gcgccatgtt gctcataacc gttgaaacga cgccggatcc
    25081 cagggtgcct ttcttctgga gccagcgggc taggacccag agaaggatgt ccccgtcaat
    25141 gatccttccc tttgaatccg ccaacaggac acggtctgca tctccatcca gcgcaattcc
    25201 gaagtgggct ccgctggctg agatcgccct ggcaaggtac ggcatatgca tgaccccgac
    25261 cccttcgttg atgttcaagc cgtccgggca attcccaacc atccggacat ccagcccgca
    25321 tcccgaaaaa agttcgccgg cgatccagct tgcagctccg ttagcgcaat cgatgacagc
    25381 aaccttcccc gatagccatc cttcaccgaa tagtcccatg agccaatcgc gatagcgcag
    25441 aatggactct ggggattgcc tgacgacgcc gatcgaggct cccgaaggcc gccactcgtc
    25501 catgagggga tccccgaggt attcttctat agcagcctcg tcgtcatcgc ttagcttgta
    25561 tcccttagaa tcgagaaact tgatcccgtt atactccgcg ggattgtgcg aggcactgat
    25621 tacggcaccc ccatggatcc ccatgtttcg aaccgtgtag ctgacgcccg gagtcggaat
    25681 cacaccaagg ctgaggacat ccgcccctgc cgaggtcagg cctgccgaaa gagcggcttc
    25741 aagcatgcac ccggactttc gggtatccct gcccagcgcc actgtaggcc gcggtatccc
    25801 cctttccgtc atgaaaagga caaaagcccg tcccaggctc aaggccattt ctggggtcat
    25861 cagccccctg ttcgcaatat ccctgacgcc atccgtgccg aacaggcaac gaatttttcc
    25921 ggtctcagac atcaccgact tcacctcatc cttttctccg ttaggggctt acggtgtaca
    25981 aaaccacgcg ttccggctcg accttcaaaa cccggacatc ctttgagaga accctgacct
    26041 ggaccggaac cacgattctt ttcgaaacca ggtttgtcac atcgacgtag gcttccacga
    26101 gcggttcgct ctggttctga aaagcgctac ttcccgattc tgaaaccacc tgaacggcca
    26161 ccgaggaggg gaccgccttc cacgaggaat agaggctctt tccctccact tttactggga
    26221 ttttaagccg tagcgaaccc gtcccgtttt caacggggcg aacgataacg gaaaccttga
    26281 tctttcctga ccccagcagc ttgatctgcc cttctggaag gatcggctcc gtttcaaagg
    26341 ttttgtcctg ggcaagtccc gtcacatcga tgggtttggt ctcaacaggt cccagtttct
    26401 cgagaacgga cctggcaccg acaacctgta cggctggagg ctgaggcacc gtttcctcga
    26461 ttcgatagcc ggcttccggg acacccgata cggtcggcac cacctggagt tcctttttct
    26521 ccacgtcctt gactaaaatc gcctttagcc ggatgactcc cgggtaaaga tccgcacccc
    26581 ttggcaggct ttcccctttt cctgtccttg gcttgacttc cagcgtgatt tcctctccgg
    26641 aaagaacttt tgccggagtt gcctgaacga agacttcatt caggctcgaa aggacgtttt
    26701 ccggcccccg gagggtgact tcagagggag tcacctcaag tttttccaaa aggacatcct
    26761 cagggaattc gccttcccac acgatcttca cgggaagcac tttttcgatc aggcgtgaga
    26821 gttcaaccgt cacctgctcc gggttgaccg aaaccaacct gacaccggga ggcaggagta
    26881 cgcgaaccgg caatcgatac ttgccgcctt ccagtccctt gagatcgact gcgcattccg
    26941 ttttgtcgag ttccaggctt tcaaaggttt tccggttggc agcgatccgg acctctactt
    27001 ccttttcccc gcccaggatg gaaaaccccg gagaggcatt ctggtagttg accttgagac
    27061 gcagtgtccg gttgacttca taccctcgat caccggaaac ataaaaccac atgaacaggg
    27121 cgacgatcaa agaaaccgct ctcagaaaca ttttcgagga aagcaggctt tcgatacgct
    27181 taaacgccat cttggccttc ctccctccac aaagccttga tttcttcccg cagctgatcc
    27241 cagagcccct tcggaccttc catggccatg aagtaatggt aaaccagccg atgaatctgg
    27301 tcttccttca gtccgcggga aaggtgaccg tttgtggcaa gggccacttc tccccgttcc
    27361 tcggaaacga ccagggcaat cgcgtcggac acttcggtca cgccgagtgc ggcccgatgc
    27421 ctcgtgccgt accaccttga caggtcggta ttggacgtta agggcagata gcaagccgcg
    27481 gccacgatga cactccgatc gataatggcg gccccgtcat gaagaggatt gttcggccag
    27541 aagattgaga cgatcaattc ctgggagatc agggcgttca atcggactgc agaccgccag
    27601 acatctgcca ggccggtctc tctctggaaa acaaggatcg ctccctgacg ctggtcccgg
    27661 agaaaagaga tggctctcgt cacatcgtcg gccatctgtt ccgcacgctc gatggctttc
    27721 cgccgcttcc agatttttcc ccggcccagc tcttccagca tgcgcctcaa ttctggctga
    27781 aacacgatgg gaatcgcgat aaaaatggct cccagtgccc tctccagcag ccaggaaaga
    27841 gccttcaaat caaagtactt ggacaccgcc gccgttgccc caatgaccag aatccccttg
    27901 accagctgca tcgcccgggt atcgaccatc aggagcaaaa cccggtaaag gataaaggcc
    27961 acgatgaaaa tgtcaagcag atcctgccac cggaagtttt ccagcatgga tgcctccttc
    28021 ggaaagcctc ttctctcaag ggaaatcctg aaggatattc ggggattctc ccctcgctta
    28081 gtttatctca attctttctt cgaggcccgt tcaataaccc cttaactttc taaggagagc
    28141 catgttcccc gcatcaccga ttccggcttt aggggtttcg aggatcaagg ggacattttc
    28201 ccaccggtcg tcattcagga tcatggaaaa aaacttcaag cccacttctc catcccctag
    28261 atgctggtgg cggtcgcgca ggcttccccg ttcagccatc gaatcgttca ggtgccagca
    28321 gccgatcctg ctgagaccga aagccttttc aagacttctt gcgaagtgct cgtaagagga
    28381 tcgttcacgg aattcgtagc ctgcggcata cagatgacag ctatccagac agagtcccag
    28441 gcgttcgtcc caatcgagcc catccaggac ttccctgaat aattcgagcc tggcaccgag
    28501 gaccgttccc tggcccgcca ttgtttccag aaggattttc actttcaggt ggctcgtctt
    28561 cgcgagcacg taccgcaacc cttccaggag gttctccact cctctttctt ccccgatccc
    28621 cagatgtgcc cccgggtgaa gcacaagttc tccgattcca agctggtcgc accggtcaag
    28681 ctcaccgatc agggcctcca cgctttttcg cctctgctcc ccttccgatg ccaagttgat
    28741 cagatatgac gcatgggcaa cgaccttcct gattccgctt tcccgtagag ccttcaggaa
    28801 tgaaatgccc tgcgagggct gaatggatgg ggccttccac tggagctggt ttttggtgaa
    28861 aatctgaatc gcctcacagc caaaggcttt gccgcgctcg atcgccttga ggaacccgcc
    28921 cgagattgaa acatgcagtc cgatcagaga cacgggtttt ccaccccagt ctctctcaag
    28981 acccggccga gccgccaaag gatcctggct gtggcccccc agacccgttg ggctccggga
    29041 agctcgaaca cggggaaaag gacctgtacc gtcccgtgct tcacccattc caggcggggc
    29101 ggagaaagaa actcctccac cctggggaga aagatttcat ccacctcgtt cgggtcaagg
    29161 cggggaaagg aaaccgctcc ggcttcctga aaggcaagca ccggctggat ctcgaaatcg
    29221 ctggtatagg catattccct ttccagcaac ccaaggaagc ggatccgggc tggatcaagc
    29281 ccgatttctt cctgagcctc gcggagtgcc gtttcaaacg gtccccggtc aaagggctcc
    29341 cttcttccgc cagggaacgc gatttccccg ggatggcggt tcaggtgcct gggacgccgg
    29401 atcaacagga tccgggtatc ttcttcaggg ccgcaaaaaa ggacaagcac cgcactttgg
    29461 cggaacttcc cctcggtggg aatcgcttcc cagtccgggc aacccgcagg gatttcttct
    29521 cgtagccttc tttcccagag cattttcctc cccttggagg cttacctgac ggtgaccttg
    29581 ccgtgataga tcagtccgcg ggagccgtcc accgtgacaa acatcccttc ttcaaggagc
    29641 ttcagggcat cggtcgccga aacgacacaa ggaatgccca tctcaagagc aagaatggcg
    29701 gcatgactgg tcaacccgcc ctcttcacag atgatcgcag aggcctttcg aatggcatcc
    29761 acataatccc ggtccgtttg acgtgccaca agcacggctc caggacgcat cttgcttcgg
    29821 gcttcggctg ccgtggtgcc cttgaccacg ggcccatacg cttcttttcg aacaagagaa
    29881 atccccttca cgacgatcct gccgaccgtg tgaacccgga ccatgtccgt cgtacccgca
    29941 atgccggtcg gcaagcctga ggtcacgacc acgatatccc cttccgaggc catcccttcc
    30001 gcgagggttg ccaagagccc cgattccacc atttcgcgtt cgtcagcatg agtctttcca
    30061 gtcaggatcg gaacgactcc ccatacaagg gcgagttctc tccaggtgcg gcgttccgcg
    30121 gttacccctg aaatcgggca accgggtcgg tacttgctga ccattctggc cgtgcttccg
    30181 ctctgggtga gggaaaggat gacctttgcc tgcatctgtt ccgcgatcgt cacagccgca
    30241 tggctaacgg catcgggaac ggtctgggaa gccaccggag gaagagaaga gggtctttgc
    30301 catacgcgga tttctttctc cgttctggcg atgatccgcg ccatggtttc caccgcctgg
    30361 accgggtatg cccctttcgc ggtttccccg gatagcatga ccgcgtccgc cccatccaga
    30421 accgcgttcg ctacgtcgtt cacttctgcc cgggtcggcc tcgggttccg gatcatcgaa
    30481 tccagcatct gggtcgccac gatgaccggt ttcccctgcg accggcccaa gtcaatcatg
    30541 cgtttctgga tcagcggcac ctcttctgcc ggaagttcta cccccaggtc tccccgtgcc
    30601 accatcagcc cgtccacgac ttcgacgatc tcctgcaggg aagccacggc ctcgcttgtc
    30661 tcgatctttg cgatgatctt catggatccg cccatttcct ccatgacgcg gcggacttcc
    30721 atgatgtcgt cccgggttcg aacgaaagaa acagcgatgt agtcaaggcc gtgttcgagt
    30781 ccccagcgga tgtcttcccg gtctttgtcg gtcagcgtcg ggattttcaa ctttgctccc
    30841 gggaaactga cgcctttctg ctctcccagt tctccgccga cgagcacttc catcaacaac
    30901 tcggtctctc ccgcttcgag aaccttcaaa tgcaggctcc cgtcgtcgat gaagacatcc
    30961 tggccctttt gcaactccct gcagacctgg ggatgactga tcgacacctg gttctccgtc
    31021 cccatccctt cttgggcgac aaaccggaag aactgccccc gcacaagggt gacgggaagg
    31081 ccgttaacca gcttgcccgt acggatttcg ggcccctttg tatcgaggat cgtcgcaatc
    31141 ggggtcctca actgttgctc cacggttcgg atgttctcta gattttcccc gtggctctcg
    31201 taggatccgt gactgaagtt caaccgggcg acattcattc ccgccttgac catttcagtg
    31261 agcgtctccc tggatcgaca ggcaggcccc agcgtgcaga cgatcttaac ctttttcatc
    31321 atgcaacctc ccgcaaagga ttttcccgtg cttggtgtag atttcagccg ctcccctgat
    31381 tttcattgcc gacgtgtgtt cggtgaactg ggaaatccag acctctcctg catcaagctt
    31441 ctcggtatgg ctaaacttcg tttcctggcc tctcgtaagc ccgatcaccg tgactccgtt
    31501 ttccatggcc ttgatgacaa tgtagtcgct caggacagac gcgatctctc ccgcttcttc
    31561 cctcatgaaa gtcccctccg ttttgaagaa ttaaaggatg acttcaagcc cttccccgca
    31621 aagattcacc aagcgctcca cgagagaggc ttccggtttc acacggatct ccttcagccg
    31681 gatcaccgtc atcgaatttt ccccaaagta ctcgacgatc acgggagaag tcccggcatg
    31741 tcccttcagc tctcgacaaa gcgccttgaa gggcagtggg cctgtttcgc cttttagccg
    31801 aatcctgaca aacggttcct gggagttctc gatctccgac agcgggatga cctcgtcggc
    31861 aaccatcgtg attccgcccc gatcctggac tttcccccgg gcgaaaaaaa ttttccccgg
    31921 atccaggatg cccttgaccg attgccagag cttcgggaag cagaccactt ccagccgact
    31981 ctccgtatct tcgaactcca ggatccccat caggtccccc cgtttcgtcg tcttctcctt
    32041 aaagctgacc agaagtcctc ccacggtcgc ctggctttca ggggctttcc aaaatcgcag
    32101 gtcggaaatc ctgcaggtcg aaaatctcgc gatccgctct tcctcctttt ccagcggatg
    32161 ccccgaaatg aaaagcccca ggacttcctt ctccaggtcc agcgtttcga gaagggtaaa
    32221 atcgtcgcac tcccccatca ccggttcctc ctggggcaat tcctccacca gtgtcaacag
    32281 cgatctctgg gctccgtcgc tctgtttctt ttgcacacgg tcgatcatca gcggcaaatt
    32341 ctcgagcagt tgtttccgat tctggtgcag cccagaaaag gcccctgcct tgatgagatt
    32401 ctcaatcacg ctgcggttga cgattcggag gtcaaccttg ttcaggaaat cccagagaga
    32461 tgtaaacgcc cctttcgact gcctcgccgc caggatggcc tccacggcag tttctccagc
    32521 ctttgcaact gccttgagcc cgaagcggat aactcccccc ctcaccgcgg agaaagcggc
    32581 tcctgactcg ttgatatcgg ggggcagaac ctggatcccc gcgtttcgaa cctccctgac
    32641 ataccgtgca agaacatcca tcctggaacc aatctggctt gacaggaaag ctgccaggaa
    32701 ttccgccttg taatgcgcct ttagataggc ggtctggtaa ctgatcaggg cgtaggccgc
    32761 actgtgggac ttgttgaagc catagcccgc gaacttctgg atgaggtcga aaagggattc
    32821 cgccgttgtc cgttcgactc cgttgctgag gcagccgtcc acgaattttt tccgctgctg
    32881 ctccatcact tccattttct tcttgcccat cgcccgccga agaagatccg cttcccccag
    32941 ggagaatcca gccagagtcg cagcgatctg catcacttgc tcctggtaaa ggatgacccc
    33001 gtaggtttcc ttcaggacag gctcgagcaa agggtgcggg tagacaacct cggatcggcc
    33061 gtgcttgcag ttgatgaatt gatccaccat gccgctctcg agcggacccg gccgataaag
    33121 ggccagaatc gccacgaggt cctcgaagga atcgggcagg attttcttca gaagttgcct
    33181 cattccggga gattcaagct ggaagacccc cagggtgtcg cccgcctgaa gcagggcaaa
    33241 cgtcgatgga tcctctaacg gtagagcgtt gatgtcgggg atttccttcc cgttcaaccg
    33301 gatgttctcg agggtgtctt cgagcatgga aagggttcgt aacccgagaa aatccatctt
    33361 gaccaggcca agtttctcga tggcctccat gccaaactgg gtaaccacct gatcatcccc
    33421 gattttccgg acgggaacca ggtcggtcac cttcaccggg gagattacga cccccgcggc
    33481 atgttgggaa caatggcggg caagtccttc aatcttgccc gccacttcaa gtaagttccg
    33541 gatcgcgggt tctttcttct gaaagagggc caggtccggg gatccttcca gcgcctcggc
    33601 aatacttcca accccgtcgg ggacactctt ggccacccga ttcacgtctg gcagggggat
    33661 ccccagcgct cgtccgacat cgcggatcgc tcctttgctt ttcatccggt caaaggtaat
    33721 gatctgggcg acgtgctccc ggccatactt gcgcacaacg tactcgagaa cttcgtcccg
    33781 cctcttgtcg gagatgtccg tgtcgatgtc cggcatgctg atgcgctcag gattcaggaa
    33841 gcgctcaaaa aggaggtgat accggatcgg atccagctcg gtgatcttca acgcccaggc
    33901 ggcaagagaa ccggccgctg atcctcttcc cggccccacg gggattccgc gcgcctttgc
    33961 cgctcggata aaatcggaaa ccacgaggaa gtacccggcg aaccccatct tcttgatgac
    34021 ccccagctcg taggacatcc ggtcacggta ttcctgcggt ggggatcctc ccattcgctc
    34081 gttcagaccg tcccaggcaa gtctctccag gtagctctcc agggtgtctc cctccgggac
    34141 ctcgaagcga ggaagccgat aaacgttgaa ttccagcttg acatcgcatt tctcagcaat
    34201 ttcaagagta tgggtcagcg cttcggggag ttcctttccg aacagggccc acatctcctc
    34261 cggagagcga aggtagaact ccgatgcccc gaatctgtag cggttgggat cattgaccgt
    34321 cgcgttggtc tggacacaaa gcaggatatc gtgccaggaa gcatcagaag cgttcaggta
    34381 gtgggcatcg ttggtcgcga ccagggggat tcccgtggcc ttcgagatgc ggacgatcgc
    34441 cttgttcacc acggcctgct cgggcagggc gttgtgcatg acctccagat aaaaattccc
    34501 tttccccaga atgtcctgat agaggatcgc cctctctgct gccccctcct cgtttccctc
    34561 aaggatcaag gcggggatct ctcccgccag gcaggcggaa gaaccaatga gcccttttga
    34621 atgccgggca agaagatcgt ggtcaatccg cggccggtag taaaatccgt ccgtgttggc
    34681 aatggaaacc agcttgacca aattgtggta tccttccagg tcccgggcca gaaggatcag
    34741 gtgattgttt cctcccttcc ggtcccggca ggtatgccct agcggatcaa ggtacacttc
    34801 gcagccgatg atgggcttga ctccgcaagc gcagcatttt tcgtagaact ccaccgtccc
    34861 gtacatggcg ccatgatccg ttaacgcgac cgcgggtgat ccccactccg cgaccctggc
    34921 tgcaatctca gaacagcgga tcgccccgtc aagcaggctg tattcgctat gaacgtggag
    34981 gtgaacaaag gggtccttca ttcccctgcg ctcccttctt caaacccgcc gattccgcct
    35041 tcctgatcat ccgtcctgac cagcaggatt tcaccgccaa accagctgag catctcctca
    35101 atcgttgcat tcctggaagc acccctggca ggttcggcct ccggccgcgt ctcttgaatc
    35161 cttttccctc gctcggagtc gtcagcctgg tccctgcgca gccaggggat cccattggga
    35221 tcttcccccg caggaaccgt ttccatggcc ggagtttcct tctcgagaaa ccggtccttc
    35281 ccgcaacaga aaatgaaggg gatctccccg agggcccctt ccttcaggac atggaagcgt
    35341 cttcggttcc gctcaagccg aatcagttca tagcagatcc ggtcccgctg gggaaaatcc
    35401 acctcgactc ctccttttcc cgccaaaaca cgggctggag caatcgccgc cacaatctgc
    35461 cggtcttccg gatcccggct ttccaacagt ccgatccaga aacggcgaat cgcatcgata
    35521 tcgatcgccg gcagcgcatc ggaggagggt tgcgtttccg ggtcagggac gaccggagag
    35581 gggcgaggga tcctttcatc ccggggaagc gtccgctcct gttggagagg catagcggcc
    35641 ccctccgaga tcgggccgga agcaagcgcg gcccgaccaa gaccctcgag aagaagtcct
    35701 gccaggacat cggtgcgcat ccccgtccgg acacggctga tcagccggga aagggtgtcg
    35761 agacaggcgc ggagtccctc caacgtccag aacggaacct caccaattag aaaggctctt
    35821 tcctctcccg aaagctcaag gccctccagg gcggtttctc cccatgcctt caggatccac
    35881 agatcccgaa agatcaggaa cagattttcc agaatcctct ccattgaggt tcccttcctc
    35941 agggactcca tgagctctga aaccgccagg tcaggcgtgg ttcggaagga tgccgtgaac
    36001 ctttccattt cgcaacgact ccctccgccc aggagggctc ttacggaatc gattgttacg
    36061 acccccgatc caagcgggat cgcctgctcg aggagcgaca gggcatctcg catcgctccg
    36121 tccgcctgtc ttgcaagttc ccagagcgcg gcctcatccg cggtgatccc ttctttttcg
    36181 caaaccaggg acaatcgtgt ggcgatgtct tttccggaaa tccttcgaaa cgggatgtgc
    36241 tggcaccggg aacggatcgt gaccggaacc ttgtgaggct ccgttgtcgc gaggatgaaa
    36301 acagcgtggg acggcggttc ctccagggtt ttgagaagcg cgttgaaagc cggttctgtg
    36361 agcatgtgaa cctcgtcgat gatgtagact ttccttcggg aggaaaaggg agcaagcgaa
    36421 acatgggctt tcagttccct gacctcgtcg atcccccggt tggaagcgcc gtcaatttcg
    36481 acaacatcga ggctctcccc cgccgtgatc gccagacagc ttgggcaact cccgcagggc
    36541 tcggccccgt gattgtcatc cgtgcagttg agcgctttcg agaggatccg ggccaaagac
    36601 gtcttcccac atcccctggg cccggaaaac aggtacgcat gcacggtctt ccctgtttga
    36661 agggaacgcc ggagcaccga aaccgctgca tcctgaccta cgacctcctc aaagcgcaat
    36721 ggccggtagc gccggtaaag ggaaacgtag cggaccggag aggaaaaaat tcctttttcg
    36781 ctcatcccag gatggctcct ttcttaaagg aggtaatgtc ccatgggatg gacccgctgg
    36841 gcttcgatca aagctttttt tagattttcc gacaggacat ccagtgagtc cattccagcc
    36901 ggcaggtcgc tttccctgac aaccgctttc tggccgaccc ggaggactcg actcttccca
    36961 tccggacgga cttcaatctc gttggacatc aactccatct ggagggactg atccagtcca
    37021 cgtttgaccc cctccatcag gactcgaacc ttctcgccga cagggccccg ccagatggcc
    37081 ttcccatcca cggtcatcac aaaccggtct tcctgggact caatggaaat cctcagcagg
    37141 tgaaggggtt ccttccgatg gagagcccag ccggaattcc ggataaactt gaccatgtat
    37201 ccgattctct ccggaatttc cgggtgatcc atgaaaacgc cgggattcac gtaagggtgt
    37261 ttcagctgct cttcggcaag cctttccatc acggtcaccg aggccgcaac cggaaacccc
    37321 gtagcatgca ggattcgaag ggcccccagg tccgcctcct tttctaggtc cctcccatag
    37381 gcattcagga tcgctacctg ggcaatgttt gccatgatca ttgcggcggc ttccccccgg
    37441 ctggcgaccg caacggccaa agcaagcagg gaaagtcgct cgtttctcgc cgactggatc
    37501 atcccatggc tgttgtccgc atggatcatc tcgtgggcca tgaccgaggc cagttcggca
    37561 tcgctttttg caaaatccag catgccggtc gtaaagtaga tgaaacctcc gggcagcgaa
    37621 aacgcgttgg gttgtttttc gcggataagc cgcacctcat agggaaaggg tttcctagcc
    37681 tccggcagga ttttttgaag gatcgcggca agccgggcaa cagggacggg gtctgtaacc
    37741 ctttcccatt tcttttcgac ttgctcgcaa accttcttcc ccagggcaac ttccctttcg
    37801 tcctctttgg agagggcacc cattgcctgg ggaggcgaaa cagcgaacag cgcgatcagg
    37861 accagaaaga cgaacgtttt tttcctcaac agggaatctc cttcccggtc atcctgatga
    37921 aggggagaaa tccttcgggg cctgaaatga gaggctgcaa ccattttacc atcaggagga
    37981 aatgggacgt cccggatcgt ttcctcgact aaccggctat gcaatcaaaa agggagtcga
    38041 gaagacagtg cccccaatct ctgaggagat cccggacctc ccggtaggtt tcaaggggtt
    38101 tgccatatgg atcggggatg tctggaagtt cgagggttcg gatgcgtgaa agctcttccg
    38161 gatactgagc gagcagcgac gccacatgct cggccgtcat gccgacgacg tggtcggctc
    38221 tcttcacaag ggtgtgagtc aactggcgac tccggtgccc ggaaagatca atcccccatt
    38281 ccttcatcac ctcaatggca tagaacgatg cggggagacc gtcaaccgtg aacaatccag
    38341 cggaagaaac ctccccctgg atcccccgtt ccctcatgag cttacggagg agtccttccg
    38401 ccatgggact tctgcaggtg tttcccgtac agacgagaag aatccgatag gggcgcgtca
    38461 acttctggct caggcaaacg atggggattc ctcctccagc atgatgcgct caaggagatc
    38521 tgccctgttc accacaccga ccaggattcc atcccggaca accggtgccc gctttaagtg
    38581 gttgcgtatc agggtaatgg ctacatagaa gtcgctgtcc tcttccgaga agctgacgat
    38641 ttctttcgcc atgatcttgc tcacgggatc ctttccgatt ttcttcagtc tcttctggaa
    38701 ttggccatag tccgggataa aggagggatc ctgcagataa tcgaagtacc ccggcagagc
    38761 ggctttcaca atatccttct cgctgacgaa gcccacaacc cttccggccc catccaccac
    38821 gggcaggcct gacacccggt ggcgagtcag gatctcgatg gcttccgaaa gggtcgtttc
    38881 agcagaaagg gcagtcaggt cccggtccat cacttcacag attctcatct cgattgcccc
    38941 ttcctacaaa agcctttcga agcggatccg ggggatctgc cgttccacgg cggcgcggtc
    39001 cctgatcccc ttttccacgt gaagcgcatt ctctagcgca gcggccatcc cgaacacgat
    39061 ccgatctttc gtttccattc tttcctggcc tccgaggatc aacccagcga tcagcgcttc
    39121 ccccgagccg aacatcgaaa cgattccctt cctgtcgaac tctgccagga aaacgccttc
    39181 atgggtacaa aatacgttcc cataggttct gtaggaagcg atcacccatt cgaccccgtg
    39241 agaatgcacc ctttggaccg cttcaatgag attgtccagg ctgttcaggg ctaccccttc
    39301 ataggtcgcc atgaatcgct gatccacttt cgcgtacgtc ggcccttcgt caagggcagc
    39361 caaaagagga gccccagccg cgtcgatcag cgtcgggatt tctctctgct ttgcgagtcg
    39421 gatcagttcg cagtagaaat cctgagggac gccgggaggg agagaccctc ccagcaccac
    39481 cagttccgtt ctcttgagga tccgttcgta attcgaaagg aaacgggcga tctcgtcctc
    39541 gctgatgtag ggacctagtt cggccagacc cgtttcaacc ttcccgacct cgtccacaat
    39601 gtagacattg gtccgtgttt ctccccggac atgcaggaag ttcgtcgtga tttgcgcctt
    39661 tctaaggacg tcccggacat actctccgct gaacccggca agaaatccca ttgccgcggt
    39721 ttctactccc agctgcttga gaatcaacga aacgttgatt ccttttccac cgggcgaacg
    39781 ctgcacccgg gagactctaa accagcctcc tggccggaat tcgggaacga cgtactcctc
    39841 gtcgatggcg ggattcagcg tgactgtgac gatcaagggc actctcccct ttcgatgctt
    39901 gcctctcttt tacaggccct tcaactcctc cgcggaatgg atttgcaaac gcttgaggac
    39961 ctctcccttc cgaatcttca aagcggaggg cagtgattgc acctgcagct tctcggccat
    40021 cctcgagttc ttgtatccat ccacgagcac cacccggttc cattctgggc tcgcttcgat
    40081 ctcggctgca agcctcacct gggcctcgtc gacgctcgag aagaaaaccg cggtgacctc
    40141 ttccggctcc aggagtttcc cttccaggtg aacctgctcc gagatgtagg cataggctgc
    40201 cattgctgcc gttgcaccat ccccagccgc agtgacgacc tgccggaggt atttatcgat
    40261 caggtctcct gcggcaaaga gcccctcgat cgacgtttcc atcctttcat tcacccgaag
    40321 ccatccacct ttagcggtct ttagacggcc cgaatcgatc agaggcttca aaaacccgat
    40381 attcggctca ttcccaatga aaacgaagac cccggatacg gggagaacgg ttttctcgcc
    40441 cgttttgacg tttttcaggg aaaggctttc caccatatcg gttccacgaa tctcctccac
    40501 gacagaatcc cagatgacgg agatcttcgg gttgctgagg gccctctcca ccgcgatccg
    40561 gtcggccctg aaggaatccc ggcggtgaat gatggtgacc ttcgatgcaa actgggtcag
    40621 gtaaaccgct tcttccactg cggtgttccc tcccccgatg accgcaactt cctggtcttc
    40681 gaaaaaggct gcgtcgcaaa ccgcgcaata actcactccc cggcccgtaa actcggcctc
    40741 ccccgggcat ccctgctttt taaaacttgc cccggttgcc agaaggatcg cctgagcctc
    40801 aacgggtccc ttgtcggtca cgacaatttt cttgccgtcc ttgaattcga tggcctccac
    40861 ttccgcctcc cggaattccg gggtaaactt ttccgcgtgt tccctgaaca tgtccgcgag
    40921 ttcctgtccg ctcgcgtgct tcacgcccgg ccagttttcg atctcatccg tcgtgttgat
    40981 ctggccccca ggcaggccct tttccagaat caggacatcc agcccagaac gcctgccgta
    41041 gatcgcggcg gtcaggccgg ccggtcccgc tccgatgatg acaagctccc tcttttccat
    41101 cgtttcgtcc tcccccgaga aaattggctt tgaaggccct tccgttcttc gattcttcct
    41161 ttcgttctat tctaagcaaa tcctaacgat ggcgccagac ggcaatccgg tccttcccgg
    41221 cccaatccct tttgacccag gcgagttcaa gagccgggct cgataccgtt ttgagcctct
    41281 cggcctgctc ttctccgcca atttcaagca gaagccatcc accgggaaga agggcgctct
    41341 ccgcctcccg cagaatgttc cgaacctgtt ggagtccatc ggggcctccg tccagcgcca
    41401 ggatgggttc gtaaagccgg acatcttcca tcagggacgg gatttccgag gatggaatat
    41461 agggtgggtt tcccacgatc aagtcgaagg ggagccaggc tgagggaatc tgtcggatcg
    41521 agctcccgtg ccaaaggagc gcccgatgct ggaaaggaaa gcggaccagg ttgcgccagg
    41581 cccaggcgag ggcaagcggg cttttttcca tcattacagc catggcttct tcccgctctg
    41641 caagaagggc aagcgctagg catccgctcc ccgtacccca gtcgaggaaa cgccccttgc
    41701 tgaaaagctc aagcgccgtc tcgaccagga gctcggtctc aggacgcggg atcaggcagc
    41761 cgcggccaac aagcagatcc agcccccaga aagaagccct gcccttcaga tacgccacgg
    41821 gaacacgagc aatccgggct tcagcgaggc tgaaaagtcg atgcacctct tcgggcttta
    41881 cctgtcgttc cgggtgggct agcagatcct cgcgagaaca cccgaacacc tccgagcaga
    41941 ggatgtcggc ctccaacgcc gctaagggaa tcccgctttt ccgaagcctt tcacgaacct
    42001 caaagcggac ttgccctcgg ttcatgcttc aggcactcct agctttccag gttcttcagc
    42061 ttttcggcct gatccgcaag gttaagggat tcgatgagtt cctggagatc cccgtccatg
    42121 acctggtcca gtttatagag ggtcagtccg atccggtggt ccgtgagccg gttctgcggg
    42181 aaattgtaag tccggatccg ttcggacctg tccgcggttc cgatttgtcc cctgcgctcc
    42241 gatgcaagag actcctgctg cttctgaagt gcactatcgt agagcttggc tcggagcatg
    42301 gccatggctt taacccggtt cttcagctga gaccgctcgt cctggcagga aaccaccacg
    42361 ccggtgggga tgtgcgtgat cctcacggcc gagtcggtca tgttcacgta ctggcctcca
    42421 gccccgctag ccctgtaggt atcgatcttg aggtcttccg ggttgatctg gacatccacc
    42481 tcgtccgctt cgggcaggac ggcaaccgtc gcggtggaag tgtgaatgcg cccgctcgcc
    42541 tcggttacag gaacccgctg gacccgatgc acaccgctct cgtatttcag ttggctgtaa
    42601 gcccctttat tgggtattct aaaagcaact tccttgaatc ccccgatccc ggtctcgttc
    42661 agggtcagca gctccgtgga ccacccctga cgctccgcga accggacata catgcggtag
    42721 aggttcgcgg caaaaagggc tgcttcttct ccgccggcac ctgcccggat ttcgacgatc
    42781 acgcttcggt catcattcgg atccttcggg atcaggagaa gcccgatccg cttttccagc
    42841 tccgagagga ccgcctccag cctttcgatc tcttctcttg ccatttcttt catttcgctt
    42901 tcgtcggtgt cgaagagttc tcgggcttcc tgcaattctc tacggaccgc ctcatattcc
    42961 ctgaacgccc ccacgatctc ggaaaggtcg gaatgtttct tggctaccct ttggagctcc
    43021 tgggggtttc ccacgacttc cggaagcgtc ataagccttt caagttcaat ataggttttc
    43081 tcaacttcct cgagcttctt cacaaaatcc atccgtcatc cctccatcaa acctagaggg
    43141 gccgagccct tccccgcttc gtttccgagt gccagttcaa gggcttttcg cgcaacgcca
    43201 gctgttcttc ccccggctcc ctggttgtca ggtactgaat ccaaagggtc gggaacaaca
    43261 gccatttccc ggccctcgga atgctcgacg cccctcggat gatttcgtaa gcgactccga
    43321 tcactagggg aaaaagaaga atgcgacagc caaccctcca gaaaagtgag ccgtttcctg
    43381 ccagtgcgaa aacaacgatg ctgaccgcaa cgatcacgag gagaaaggac gtcccacacc
    43441 gcaggtggaa ccgggaactc gatctcaagg aacgaaacgt ccccatgggg aggccggctt
    43501 catacgcatt gatcaccttg tgctcggcac catggtattc aaacaccctt ctcatgtcct
    43561 tccagagtcc aatcacagcg atataggcga tgaaaaccgc cgcacggatc aatccttcaa
    43621 ggatccgccc ttcccaggcg tttccccagt cctgtccgta aaggcggtcc gtcagccaga
    43681 ccggaaaggc gacaaagagg gctatgacta gggacaaagc aattcccacc gtgagggcca
    43741 tttcctttgc tgaaacctgg ccatcctccc ccagggcgac ctccgcggag agcgcaaggg
    43801 ctttgtagcc gatcgtcagc atctcggcca ttgtcgccag tccacgaagg atggggagct
    43861 tccaaatccc cgcagccgcc cagggcacgt tgggccagac cttttcgaaa agggtcccgt
    43921 cttcctttcg gacagccagg gcccactgcc ctctccccct catgaggacc ccttcaatga
    43981 ctgcctgtcc gccaaccgga agcctttctg gcttttcggc agattctccg accgccgtta
    44041 ggctgccgag caaaaaaaga cagccagctc taaggttttt caaggaggtc ctccatgctg
    44101 gaataaggct caccgcaggg ccgctcctcc gggcagccac ccttcgtcag acaaccgggg
    44161 cctgctacgg caaacaggag gggggctttt tctctcgcga ggcacagcat ctgtctggca
    44221 acttcccgga tttcccactg cgctcttttg cacagccgga gggaaaagaa gtggagaagc
    44281 tctctggcgt tcatcgtcac gacgagatgg gtgacacagg cattgggcag cacgtaccgc
    44341 gcatcttccg ggggcactcc ccgagccagg agttccccgt aagcagcgta ggccattcgg
    44401 atcgcctctc tgaaaaaggg aagcgcctcc gaattcgttt ccacggtttg cggggtgacc
    44461 acttccgcct tttcagcgcg gacatatcgc tggctctgct ggctgaagct cgcaatccgg
    44521 tgccgaacca gctggtgcga gcaggcccgg ctgatcccgt ctaccccgaa cacgaacgag
    44581 gcgtgctcaa agggggaaaa gtgtccactt cgagagagat gtttcaagag acgatccgtc
    44641 tcttcgggtt ttaactcgga aacgagctcg cttgcagatc ccgggctgta gcatagccgc
    44701 gcagcggcag cgacgatcct gtccgcttcc ggggtccacg tgattagccg aaccaaaggg
    44761 ggcatcgttg cgcttccttt ccttatgaga aaggggagct tgcgctcccc cttaattttc
    44821 ttcttctttc ctctgaccgt aatccacccc ggcgtatttc ttttggaatt tctccagcct
    44881 ccccgcctcg gcagagacga agcgaccctt cttgccggtg tagaagggat gacaggcgtt
    44941 gcagaccact accttcagct ctttgctcgt cgagcgggtc tcgaatgtgt tgccgcaagc
    45001 acagacaacc ttgcattcct catagcgggg atggatgtcc tttttcacgg tggagcacct
    45061 ccatacggac ttttctaaga ctacacgcag aagcaaactt tatcacagac acttgtcgat
    45121 ggcaagggaa catcagatgt gcatgactcc caggccgcac ctttcatggt gagccacggc
    45181 gtgatatgcc gtgtcgccga ccatcgaaac agcaatccag ttcctgttcc tgacgattgc
    45241 cagtttcgcg ccgatgctgg cctccagcgt tccaagagaa acaaagccgt tgatggcctc
    45301 aagagccgcg tgcatcaggg catgcatctc cctgggcttc ttatcgacaa ctccggcatt
    45361 cagagcggcc cccaccagag ccctggtgat tttctgcggc aattcgctcg ccactccgcc
    45421 gacttccgtc gccacggact tccatccctc ctcgccaagc agggctttca actcttcctc
    45481 cgacttcttg tcggtcgtgc aggagagcaa caaagccgca cggcctacct ggctttcctc
    45541 cgtaaatttc aatctcaccg gcagttcgaa cccgcgcatc gaattttcct gccgcccgct
    45601 gtcttcctgg attggttcag cttgctgtcc atccagggag gtgcctttca ccccgaaaat
    45661 ttccctgagt ttcatcctaa aaggcctccc tctctctacc ctgagttgaa acgggattat
    45721 tataacgcat gtccagagcc ttcctggcag ccggttgaga tcctcccctt ttgatgataa
    45781 aatgccaagt ggagtttca
//
LOCUS       DAOOVN010000005        44887 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_529_length_44887_cov_14.112665, whole genome shotgun sequence.
ACCESSION   DAOOVN010000005 DAOOVN010000000
VERSION     DAOOVN010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 44887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44887
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_529_length_44887_cov_14.112665"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="PLY83_01165"
     CDS             <1..512
                     /locus_tag="PLY83_01165"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOV28677.1"
                     /translation="RMDRMAEMLACSPEETVLDLGGGTGLFACRLVDRCGEIHLLDES
                     LRMIQQGSCGKIRVRQGDATDTGYPERFFDWVVLSDVIHHIREQDLLFREVARILKPG
                     GKLLVNEIDREKPLGRLVGKLERLVFPPVYHIGFKELSNSLLRYGFTLRQQVQDAWSF
                     IALWDNSGG"
     gene            complement(509..1135)
                     /gene="hisIE"
                     /locus_tag="PLY83_01170"
     CDS             complement(509..1135)
                     /gene="hisIE"
                     /locus_tag="PLY83_01170"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HOV28678.1"
                     /translation="MSLLDQIRFDSAGLVPVVVQDCESGEVLMVAWTNREALQETERS
                     GELTLFSRSRNRLWKKGETSGNTMRVVEMKIDCDGDSLLALVEPAGPACHTGQRTCFY
                     RSLWGEGGTVCTFWGRLWRWLLERRHAPAEESYTARLLQEGSSRIAQKVGEEGVETAL
                     AVALKDREGTRYEAADLLYHLLVAFISLDLKPTDILGELSRRHHSAKD"
     gene            complement(1132..1911)
                     /gene="hisF"
                     /locus_tag="PLY83_01175"
     CDS             complement(1132..1911)
                     /gene="hisF"
                     /locus_tag="PLY83_01175"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOV28679.1"
                     /translation="MLMKRIIPCLDIKGGRVVKGIRFRNLRDAGDPAELAAAYMEEGA
                     DELVFLDISASVERRRTMTDWVKAVAERLFIPFTVGGGITHADEARHLVALGADKVSL
                     NTGAVRNPTLIRDCARTLGRQAVVLAIDAKRKPDGGWEVLVEGGRTSTGLDVLDWARE
                     GVRLGCGEILLTSMDQDGTNEGYDLELIQTVSETAPVPLIASGGAGSAEDLRLAFEAG
                     ADAALAASIFHYGVLSIREVKLELAKWGVPVRIGSEEGKSR"
     gene            complement(1905..2624)
                     /locus_tag="PLY83_01180"
     CDS             complement(1905..2624)
                     /locus_tag="PLY83_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HOV28680.1"
                     /translation="MRIWPAIDLYQGKVIRLTRGDYSARTEYADDPLSVASAFKSCGC
                     PRLHVVDLEGAQRGTPSHADILAPLSALNLTIRYGGGLRTPASVGRVLEMGAAFAMVG
                     SLLFATRETPFDLFRKFGERVIPTIDIKKGQVAVSGWTEKAALGPEEALHRLFMAGFR
                     HCLVTAVDRDGTGEGPDLGLYSTLREAFPGMQFTAAGGIRCLKDIQALAELGISDVVI
                     GKALYEGDLKLEDALEVERQC"
     gene            complement(2621..3241)
                     /gene="hisH"
                     /locus_tag="PLY83_01185"
     CDS             complement(2621..3241)
                     /gene="hisH"
                     /locus_tag="PLY83_01185"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOV28681.1"
                     /translation="MIGIIDYGAGNLGNVLRALSSLQYEASLLGSPSNLGDQVDLLVL
                     PGVGAFPPAREELARRGWIPVLREWGNTGKPLLGICLGMQLLYEASLEDGLTEGFGFF
                     PGTIAPLAGLKKTPHMGWNRVSWLRELPGITDLFQGGLDAYFVHGYAAPVNEASLADT
                     AIDGIRFSSVSLQGKIAGFQFHPERSGREGLQLFGTFIRFLSGKSS"
     gene            complement(3238..3807)
                     /locus_tag="PLY83_01190"
     CDS             complement(3238..3807)
                     /locus_tag="PLY83_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HOV28682.1"
                     /translation="MEKRYERRSRETAVEFEFLPPGETVRVETPIGFLNHMLGLFLHH
                     ADVPGKLKASGDTEVDAHHLTEDVGIVMGSAFLDLFRSGPCERYSSIALPMDGTLVLV
                     AADISGRGGLTWNAVFPTQRCGEFDTELVREFWTAFAREGKVTLHIHALAVDNSHHLA
                     EAIFKGTGRALRAALVPSSREASTKGLWI"
     gene            complement(3791..4453)
                     /gene="hisG"
                     /locus_tag="PLY83_01195"
     CDS             complement(3791..4453)
                     /gene="hisG"
                     /locus_tag="PLY83_01195"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOV28683.1"
                     /translation="MLTLCLPTGRVLDEAVEVLENLDIPTQRLRDRGRSLVIQEGDFR
                     FLLAKPMDVPVYVHYGIADLALAGSDVIWETGASLVELADTGRGLCRIVLAGPKKVAA
                     RFLGHESRLMGLRVATKYPRIAEAFFADRGIQVEFVHLNGSIEMAPRLGLSDCIVDIV
                     QTGSTLKANALSVIEEIAPVSLKFVASCKSLQLAWNGIEEIIEKFSGSSKKAGDEDGK
                     AV"
     gene            complement(4447..5628)
                     /locus_tag="PLY83_01200"
     CDS             complement(4447..5628)
                     /locus_tag="PLY83_01200"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HOV28684.1"
                     /translation="MSRQPKGTRTLGGREGERVERCRKVFFETFSAYGYQPFYAPGLQ
                     LFESAWEKLPPGFRKRLIPVYTPSGEPCFLRADLTLAAVAYLSSHFSPQERPLRVCYA
                     GPLYRAPIPPENDFEKMQFGAELLGWEGQGADVEVIALLLRSLRRLGIHSPVVVLGHA
                     GFLRESLSRLDRTLSSLLTLALLERRYEAFFNALGEAHLPSGTEDPLFQLPYLRGNAS
                     RVLKEATRLFGETQPLRDLSLIVEALEELGLGENLRLDLALARELGYYSGPVFEIFSG
                     SAGFSLGGGGRYDGLLEQYGIVGQALGFGLDLRTVASLSRFEGPTPAVAWCGELSPRV
                     ALERADDFIASGYRIELSWQENREGAIQLARSRNYSWWIDLPACTAYPLDSMEGEESG
                     C"
     gene            complement(5625..6887)
                     /gene="hisD"
                     /locus_tag="PLY83_01205"
     CDS             complement(5625..6887)
                     /gene="hisD"
                     /locus_tag="PLY83_01205"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOV28685.1"
                     /translation="MIIVKAPASSDRESIDRSIQERVHSILQRVFEDGEQALLELNRH
                     LDGYEGPILVREKAMNEALAQMDPSLRQAVETAIDHVRAFHRAQREMFRNQEWEISPG
                     IRAGMRFLPVRSAGVYIPGGRYPLISTAVMGIIPAQEAGVSRIVAFSPPRGSSGISKP
                     ILATLCLLGLKEVLAVGGAQGVGALVRGLPGIPPVDLLAGPGNAYVTEAKRQMFGRVG
                     IDSLAGPSEVLVIADHTANPCVLASDLLAQAEHDPLARVSLYCLEESLAKRTVAAVED
                     LLQTLPTSKTAQQAWEASGTVAFCTLEEAVNAANRVAPEHLELAVQNPREVLEKCTAY
                     GAAFLGHHSAEAFGDYIAGTNHVLPTEGRARYSGGLWVGTFLRPLTHLEMTKEAAASL
                     SKPGECLALAEGLHAHALAMRLRGGPGN"
     gene            7096..8442
                     /gene="radA"
                     /locus_tag="PLY83_01210"
     CDS             7096..8442
                     /gene="radA"
                     /locus_tag="PLY83_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOV28686.1"
                     /translation="MARKPGEKRFRCSECGFFALTWSGKCPACGSWGSFEEERAEFTS
                     SDLASKTVTAVSGLFDLALPGRIPSGIEELDRVTGGGWVEGSVALLAGEPGIGKSTLL
                     LQTCAAISRAGYRVLYVSGEESAPQVALRARRLGAADSGVSVLCQESVQEILPLLQKY
                     DFAVFDSVQALRHADTPGWPGTPGQVRAVAQVISEEAKRSGCSAVLVGHITKDGQIAG
                     PKLLEHMVDVVLYFTGDRTSPYRHVRGVKNRYGSTDELGVFEMAEAGLVGIPDPSRLY
                     WNENEEPIPGTAVTVVLEGARPLVAEIQSLSCTTPFPYPKRTARGVSISRMGLLLAVL
                     ERRGGLPSRSMDVFMNVVGGLHIEDPAADLAVCMSLASSLTDLPLPRDTVFIGEVGLG
                     GEVRPVPRIATRIRESARLCFSTVVTSRYQDQDVPAGIRIVKVGSLGEAMKELTGG"
     gene            8439..9794
                     /locus_tag="PLY83_01215"
     CDS             8439..9794
                     /locus_tag="PLY83_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HOV28687.1"
                     /translation="MKRLIKAALAVFLVVFPLWGIAESAPLSSEKTAVVVSVPMEGVV
                     GVSLENYLEEAFAYAVKEGAALVVISLDTPGGLVTSMRGMSQKILSAPIPVAVWVGPT
                     GARAASAGAFLVMAAHIAAMAPGTNIGAAHPVSASGQDVPDSEMNRKITNDLAAQMRS
                     LAEERGRNSGVAASMVLNSVSLTAREALEKKVIDTIAPDVPSLLKWADHRKVRVKTAD
                     VVLDLRRYEIRTFEMSARLRALHFVSRPDVAYLLLTLGILAVVFEVLTPGGFVLGTAG
                     AVMALIGAYGLRMLPFNWAGIILLAAGIVVLVLDLVVGGIGVLSLFGLASLIVGSLVL
                     FRAPGGELLNVSFGFMAGVALTLGLFFILAAGAVWRSTRKRVTSGQEGMVHDEGVVVE
                     DLAPYGFVSCHGEVWKAVSEDGASIPKGTRVRVSRAEGLLLHVREERAESLKEKGAEH
                     K"
     gene            9791..10588
                     /locus_tag="PLY83_01220"
     CDS             9791..10588
                     /locus_tag="PLY83_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HOV28688.1"
                     /translation="MIGSLIDFLFNAGSTLGILLIALVLLTSAVKIIPEYQRGVVFRL
                     GRLVGAKGPGLVLIVPFVDRMVRTDLRVVTLDVPVQEVITKDNVPIKVNAVVYFRVMD
                     PNRSVVEVENYIMATSQLSQTTLRSVIGRAELDEVLSERDKINLELQKIIDEKTDPWG
                     IKVSAVEVKELELPEGMKRAMARQAEAERERRAKIISAEGEAQAAERLSEAARIMAVS
                     PITLQLRYLQTMREIASEKNSMTLFPFPFEIVKAFMERANPEKGPLG"
     gene            10717..13197
                     /gene="leuS"
                     /locus_tag="PLY83_01225"
     CDS             10717..13197
                     /gene="leuS"
                     /locus_tag="PLY83_01225"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOV28689.1"
                     /translation="MPYDFERVEKKWQQYWAENRVFDVERDPGKPKFYCLEMFPYPSG
                     ALHMGHLRNYSIGDLLSRFLWMKGWNVLHPMGFDAFGLPAENAALKYGVHPKKWTWDN
                     IEHMTAQLKSMGCSYDWRRRVETCAPEYYRWTQWIFLLFLKNGLAYRKHAPVNWCESC
                     QTVLANEQVIDGGHCWRCGSVVTKRKLEQWFLNITRYAQELLDSLDNLPGWPERVKLM
                     QRNWIGRSEGVRLTFPVEGMEKGLETFTTRFDTIYGVTFLALAPEHPMVEEFARLSPN
                     GEQIRAFVKETIQKSEIERTAVGGEKKGVDTGFKAINPVNGERVPIWVSNYILIEYGT
                     GAIMGVPAHDQRDFEFARKYGIPIRLVIQPEAGALDPETLSHAFEEGGIQCNSASFDG
                     LPTRESIPRMAQWAEETGIGKREVNYRLRDWLISRQRYWGAPIPVVYCEKCGIVPIPE
                     EQLPVLLPDVQVLPGGKSPLPETPEWVNTTCPACGGPAKRETDTMDTFICSSWYFLRF
                     TSPWTQDGPFRGEDVNYWMPVDQYIGGIEHACLHLIYARFFTKVLSDLGYVEVREPFT
                     NLLTQGMVIKDGSKMSKSKGNVVDPDEIIRKYGADTARLFILFAAPPDKDLDWSEKGV
                     EGTHRFLKRVWRLVEEGGARLKEAPRRRIPLAEIEERSRRDLKRRIHSTIENVGRDIE
                     KERQFNTAVARLMELSNDLSDFVPETDQDWILYREGIETLLLCLSPFTPHLCEELWEM
                     LGNRDCLAKAAWPEVEADCLEAESVTIVMQVNGKVREKIELPASLSEEQLKERVLSHE
                     AVRKRLEGKEILKIIAVPDRLVNVVVKG"
     gene            13197..14147
                     /locus_tag="PLY83_01230"
     CDS             13197..14147
                     /locus_tag="PLY83_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV28690.1"
                     /translation="MPHLIIAGAAGTTQRRLVSETVRREREIGYPLAARREGGDWREL
                     LSAENCGGLFDARRLVLVEEAQQLGKFPIELESLLENASSATHLLLVYDEDPKAVFPK
                     ETHSRITWLKGEEIPRFGAARRRWAEERASEMGVLLSEDAMGLLLESVEDPEELVSEL
                     AKLAVAAAGRRVEVEEIRALCFDEGEKALLSLLDGLCEADRGKVTTVLPHVKRRELIP
                     VLAALHNRFRLAFYRSVFKAPRLLKTVSEALKARPYQERQAAEATRHYSREKMALFLK
                     QLCGINMGEKTGNRNGWIELELAILGLLADCKPAERKERR"
     gene            complement(14158..14433)
                     /gene="rpsT"
                     /locus_tag="PLY83_01235"
     CDS             complement(14158..14433)
                     /gene="rpsT"
                     /locus_tag="PLY83_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOV28691.1"
                     /translation="MPNKKSAEKRVRISERNRLYNRFWKSRCKTAAKRLLESLEGKDP
                     QIIKARLDEAQSVLDKAAGKGVLHPNTVARRKSALTLKAKEAMKADA"
     gene            14572..15498
                     /locus_tag="PLY83_01240"
     CDS             14572..15498
                     /locus_tag="PLY83_01240"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HOV28692.1"
                     /translation="MEMSHLGQGIKGGRGLVTAGLVILSVAGFFAENRGIALGKLLWL
                     AGGSGIVGLMTNVLAVRMIFHKVCLRFGAWQWILPGSGLVTRNQEKILETLARELRER
                     LLTQETLERAVSEGKLLEKTGPTFRKEVRRIFLRKSNLRKMATAVEGPLQRLLLGQET
                     ERFVRLQVEKALQDSLLLGMAGKLGLIKSEKVAEWILRSASESLNRSLVTEAGLRATQ
                     RKLAFLLENTARLGDAEILEMVKDSLERLLIRMLREIDLGELAKRSLRQATPEQLEDY
                     LEALARKYLDWIEIWGGLMGAALGLVLWVLSK"
     gene            15510..17414
                     /locus_tag="PLY83_01245"
     CDS             15510..17414
                     /locus_tag="PLY83_01245"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOV28693.1"
                     /translation="MEAYFREDGILSRMQGFEFRPQQLKIAVKVWDLLQSGNADTLAI
                     EAPTGIGKSFALLVPSLLWATEADRTVLLLTAGHALQGQLMTKDLPLLEGFLRHPFRF
                     GTLKGRNNYLCLRRCAELERGGFLSFGDEGEISEKVLEWSRESTTGDLSELELPETHP
                     VLERIAATREGCLGRLCPLKGECFLQRLYLQAQDWHLIVTNYHLFFSHLDGGKRFFPV
                     HFNALLCDEAHRIPEAARSSATRAASPERFRKMFSARPLSNLVKAFRFLKLPDGKLES
                     GWQQAAQGVEGVFNRLALLSREEGVFTRPLTDLRHDLESSVESLATEVERFLARMMEE
                     GEAPDVAEGFLVASQLVEALDDLRWCLDVVDYPRWATWWDGAQIQSTPVLAAAPVREA
                     LSSLDDVPIVCTSATLTVGGDFGFWVRESGISPAETLTLDSPFDLPSQMSIWVIDLGQ
                     RVTDKGYDSTASRVIEKLCDLNGGRTLVLCSSMRLMRAVSARLKSKERGYRLLCQGEA
                     PKEELLEDFRSDETSVLVGSLSFREGIDIPGDGLTQVILDRIPFPHPADPLVRARRDL
                     EGKDTFLRSTLPMSRLFLRQAFGRLIRRKTDAGRVAILDGRALQRDDWGIFQDFRGVP
                     VKRVRLVQTP"
     gene            17507..17641
                     /gene="rpmH"
                     /locus_tag="PLY83_01250"
     CDS             17507..17641
                     /gene="rpmH"
                     /locus_tag="PLY83_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOV28694.1"
                     /translation="MKRTFQPHKTRRKRTMGFLVRSSSPTGRRVIRNRRRKGRKRLAV
                     "
     gene            17642..18070
                     /gene="rnpA"
                     /locus_tag="PLY83_01255"
     CDS             17642..18070
                     /gene="rnpA"
                     /locus_tag="PLY83_01255"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOV28695.1"
                     /translation="MDRLFGFPADFRLKKGWEFDRVFRTGRRVKGELVRLLFLDTGEG
                     PPRLGAAVGKRQGASVVRNRGRRILREAFRNCRPWLKEGIWIVAMLRSAGLCAGAGEI
                     YEDLVRLLEREGLLRPGFPTHFPPNGKTNDEEIGAGGASS"
     gene            18048..18299
                     /gene="yidD"
                     /locus_tag="PLY83_01260"
     CDS             18048..18299
                     /gene="yidD"
                     /locus_tag="PLY83_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOV28696.1"
                     /translation="MPAALALSLIRFYQRFLSPLLGKNCRFLPTCSAYTFEAIERYGF
                     LRGVYLGIRRILRCGPWCAGGYDPVPDLEKEKRAKEFED"
     gene            18321..19106
                     /locus_tag="PLY83_01265"
     CDS             18321..19106
                     /locus_tag="PLY83_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HOV28697.1"
                     /translation="MGTLWNQAGELLLKILNLFYALTHSYGIAIILLTILVRVVLHPL
                     NQKQLVSMQKMQKIQPRIKTLQEKYGHDKQKLNEETMKLYKENQVNPAAGCLPLLVQL
                     PILILLFRVLTNFKFGGVSFLGVGLEESVLSTMGKAVGLTGQTPGITALLTGIAANPS
                     GLLNVGMYAPNLILLVSIGFLTWFQQKLSASNNPQMAFMNWFMPVFLTFICLNLPGGV
                     LLYWGVSSLLSVVQQWRVVKVTELEMEKKPVLFKNKPTRDGDS"
     gene            19140..19781
                     /gene="jag"
                     /locus_tag="PLY83_01270"
     CDS             19140..19781
                     /gene="jag"
                     /locus_tag="PLY83_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HOV28698.1"
                     /translation="MERTEPLVVETDSIENAVLEAAQNWKILPEDVQVRILEEGKRFL
                     GFFARKTRVELFPSGDLELLKAKRILSGILEQMGMDVEIHLGEEELLNITGTDAGIVI
                     GRYGETLKALEYLVNLISREGGPSRPIRLDSDGYRERREENLKRMALASAREAIRRRR
                     PVTMEPMTSWERRIVHLALKDTLEVDTRSVGDEPQRRVVIWPRPGSRQKNRSS"
     gene            19900..>21316
                     /locus_tag="PLY83_01275"
     CDS             19900..>21316
                     /locus_tag="PLY83_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOV28699.1"
                     /translation="MERIRKIGLVLGIALLFIGIVYTAPQSFKPVVTPAVSQDVFSGN
                     PVAAIASKSSPAVVNIDTKTLVRRSFSPFGDDPILRQFFGDQMDLFSRVVPMEGKGSG
                     FIVSPDGMILTNNHVVDGADSVTVTLADGRRFDAEIVGKDPTYDLAVIRIKASNLPVL
                     KMGDSDRTQVGEWVVAIGNPFGLEHTVTVGVISAKNRSIRLERLNFDGFFQTDAAINP
                     GNSGGPLINLDNQVIGINTAIVPYAQGIGFAIPINVAKQVMNDLVRYGRVKRAWLGVY
                     MQPITPDIVEAFGLKSSDGALVSDVVPDSPAEKAGVQRGDIIRKAGSVPVKSPDDLSN
                     AVRKQAAGDSLKLEIVRDGKPLSLTAKLEEMPSERSLGGQPQGRIGNRLGLQLGNVDL
                     KNRQRFDLPAIQGVVVLGVEDGSPAQKAGLQPGDVIQGVNGRVVRDLVSWERAMSQVS
                     GKNLVLLVWREGRTTFVPLKAR"
     assembly_gap    21317..21416
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21417..22048)
                     /locus_tag="PLY83_01280"
     CDS             complement(<21417..22048)
                     /locus_tag="PLY83_01280"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOV28700.1"
                     /translation="MDEERTRETQSREETAEQETLRALAEYFEDLNLDRPGPLPPLPG
                     KKEDRDRLEAALASFSERFRQAIGNLRRYTSNVSHELLNPLSVIRSKAEVTLLRPRST
                     EYYEKKLAEIIDHVSSMRNMIESLLELSRLDLAEKPLELERVNLMHIADVACQNLVPI
                     FNRRGQEVLKEVKPASLLGREPLLLTLACNLLDNASKYSPPGGRLGIRTYP"
     gene            complement(22075..22755)
                     /locus_tag="PLY83_01285"
     CDS             complement(22075..22755)
                     /locus_tag="PLY83_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOV28701.1"
                     /translation="MKLLIIEDNKELVEVLSEGFSENGFTVDFTYDGQDGLKKARAGD
                     HDCIILDLMLPKLSGLEIISALREEGKKVPVIMLTARDSIEDRVTGLEQGADDYLIKP
                     FDFRELLARVNALIRRVSEPKPALLRCGPLTLDPLARECRVGEETVPLRRREFDILEL
                     LMRHENQVFTRDKIISNVWQEEYEGTSNVVDVHVKYLRDKLRPFGLDLMVVTVRGVGY
                     KVVCPDQA"
     gene            22954..23220
                     /gene="rpsO"
                     /locus_tag="PLY83_01290"
     CDS             22954..23220
                     /gene="rpsO"
                     /locus_tag="PLY83_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOV28702.1"
                     /translation="MIEREEKQRIIEEFKVHGADTGSPEVQVAILTHRIRSLTEHLKI
                     HKKDFHSRRGLLMMVGQRRKLLNYLKTRDFNRYQTLIQRLGLRH"
     gene            23338..25635
                     /gene="pnp"
                     /locus_tag="PLY83_01295"
     CDS             23338..25635
                     /gene="pnp"
                     /locus_tag="PLY83_01295"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOV28703.1"
                     /translation="MVQTFSIEVGGRALSFETGRLAKQASGAVQVTYGETVLLVTACM
                     TEKPREGLDFFPLLVDFEERFYSAGKIPGGFIKREGRPSETAILSARIIDRSIRSLFP
                     EHMRNDVHVVATVLSVDQVNPPNIQAINAASMALTLSEIPWNGPVAALRVGCIDGKFV
                     VNPTEEELPLSTLDLVLAGHMGGITMVEAGAKEVPESLMVDALEFGHEEIKRLVRFQL
                     EVREKAGKPKVEIPAPVRIEALDEAIRKNYYGEIYEAVGIHAKGQRNEKIALIKQKAV
                     ETLEGEFPDSSRYIGEVVEELVKKAMRSMTVDERRRADGRTMDQVRPISCEVGVLPRT
                     HGSALFTRGETQALVTTTLGMMGEDEQVLDGLKIDEPSKRFMLHYNFPPYSVGEVRPM
                     RGPGRREIGHGALAERAVRHLMPSEEEFPYIVRVVSDILESNGSSSMASVCGASLALM
                     DAGVPIKRPVAGVAMGLIKEGEKVEILTDIQGLEDHYGDMDFKVTGTEVGITALQMDN
                     KAGGITREILEKALGQAREGRLHILGEMAKVIREPKKELSPYAPRMYTTSVPVDSIRD
                     VIGPGGKVIRGICADTGVKMNVEDDGRIFIAATNEESAKKALQIIRDLTRDAEPGEIF
                     YGKVTRLLSFGAFVEVLPGKEGLLHVSEISTHHVPKLEDAVKPGDMVLVMVKEIDDMN
                     RVNLTRRRLLEKAAEHRLAELYPDAYALELAREERIAKASAAAGSERPRRGEGETRDR
                     RPGGERHDRRGSGPRDRGRGPVERH"
     gene            25622..26068
                     /gene="dut"
                     /locus_tag="PLY83_01300"
     CDS             25622..26068
                     /gene="dut"
                     /locus_tag="PLY83_01300"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOV28704.1"
                     /translation="MSDIRVKVKRGENAKQIPLPQYATSGSSGVDLRSAEAALIEPGT
                     WAILGTGLHLEIPEGYEAQVRSRSGLALSRGVMVLNTPGTIDSDYRGEVRVILMNLGK
                     APFQVEPGDRIAQMVFCPVARAELEEVDTLSSTEREQGGFGSTGSK"
     assembly_gap    26372..26471
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<26472..27900)
                     /locus_tag="PLY83_01305"
     CDS             complement(<26472..27900)
                     /locus_tag="PLY83_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOV28705.1"
                     /translation="MTENTNEQWSSRWGLVFAAMGMAIGTGNIWRFPRVAATNGGGAF
                     YIAYFLALFLWAIPLLAAEGVWGKVSRMGVIGSFKEMLGKKWTWVGALVAWISLAIAF
                     YYAVVIGWCFRYFLYAATGVLKPGLDSKALWDAFINNPTQGALFFIIALLVSILIVSR
                     GIQNGLEKANKIMIPSIFILLLFLAIRAQFLPGAWKGLEYLFTIRMEDVLKGRTYLEA
                     FTQVAWSTGAGWGLFLTYFVYAKKNEDILLNSATTAFADTAAASLAGLCVIPTIFAIA
                     PDPMAAVKSGNNGLAFIHLTNLFTQIPGGIIMALAFFAALIMAALTSEISMIQLGVRL
                     LEDAGWGTKKATWAIGAACLVCGLPSSMFNWFFENQDWVWGVGLLLSGLLFSIGAIKI
                     GIRKIWVEYIEPDSDIKHYWMWWLICLFPVWFVIIFGWWTWQAISWYPGEWYKWLPIS
                     KYTYTVGTMYYQWIIAAVVFFFLNNW"
     gene            28210..>29083
                     /locus_tag="PLY83_01310"
     CDS             28210..>29083
                     /locus_tag="PLY83_01310"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOV28706.1"
                     /translation="MPQFTGKEGQVLETDHRSAWEILKEWGIHRGAVAAFVNGKPTDL
                     KAVLDFDATVEPILADSETGLEILRHSASHLMAQAVLHLYPGTKVAIGPSIKDGFYYD
                     FELPVPVSEEDLPKIEEEMKRLAREALPVERLEIPREKLMEKYRAAGEVYKAELLADI
                     PDETISVYRQGDFEDMCRGPHVPDTSFLRHTKLLSVAGAYWRGDEKNRMLTRIYGTAF
                     ADREALEAHIKKMEEARRRDHRKLGKELDLFSIQPEGPGFPCFHPKGMVILNTLIDFW
                     RREHVRRGYSEIRTP"
     assembly_gap    29084..29183
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <29184..30260
                     /gene="thrS"
                     /locus_tag="PLY83_01315"
     CDS             <29184..30260
                     /gene="thrS"
                     /locus_tag="PLY83_01315"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOV28707.1"
                     /translation="DFWRREHVRRGYSEIRTPLILDKDLWVRSGHWDHYKENMYFTEI
                     DEKPYAIKPMNCPGGLLVYKSSLKSYRDLPLRMAELGIVHRHERSGVLHGLMRVRCFT
                     QDDAHLYVTPAQIKQEILGIMDLTRYIYRDVFGFNYHVELSTRPEKAMGSLEMWERAE
                     TALEEALKESGVPYRVNPGDGAFYGPKIDFHLEDCIGRTWQCGTIQLDFQMPERFDIT
                     YVGADGGQHRPVMLHRTVFGSLERFLGILIEHFAGAFPYWIAPVQVKVIPVKPELEEY
                     AKEMEARVKAWGVRVEIDLRDEKLGKKIRDAQVQKVPYMVVLGEREMAEKTLSVRDRS
                     LGDLGTMTLEAFKEKLDLEFDPRH"
     gene            30494..31051
                     /gene="infC"
                     /locus_tag="PLY83_01320"
     CDS             30494..31051
                     /gene="infC"
                     /locus_tag="PLY83_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOV28708.1"
                     /translation="MNEEIRAQEVLLIDDQGVKVGVVPTREALKLAEERLLDLVEVAP
                     QATPPVCRIMDYGKYKYQIQKRDKDARKKQKVQTVKEMKMRPKIDEHDYNFKVRAIRG
                     FLESGHRVKVSVFFRGREMAFLDRGKAVLEKVEKDVAEYGKVEMEPRMEGRYMRMMVA
                     PVPSTKKPASKAEGSKLAEEGENRE"
     gene            31114..31311
                     /gene="rpmI"
                     /locus_tag="PLY83_01325"
     CDS             31114..31311
                     /gene="rpmI"
                     /locus_tag="PLY83_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOV28709.1"
                     /translation="MPKMKTHSGAKKRFRTTGTGKVTYQKGGRRHILESKNSKRTRTL
                     RRKGYLSGAIEDRVKVLLVSR"
     gene            31355..31714
                     /gene="rplT"
                     /locus_tag="PLY83_01330"
     CDS             31355..31714
                     /gene="rplT"
                     /locus_tag="PLY83_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOV28710.1"
                     /translation="MARVKGGSASNSKRKKLFSITRGYWGRKKNVYRRAREAFLRSMT
                     NAFRDRKAKKREYRKLWIIRVNAAARMNGMSYSSFMHGLKLSGIELNRKILADMAVND
                     MPAFVKLVEKSREALAR"
     assembly_gap    31865..31964
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <31965..32933
                     /gene="pheS"
                     /locus_tag="PLY83_01335"
     CDS             <31965..32933
                     /gene="pheS"
                     /locus_tag="PLY83_01335"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOV28711.1"
                     /translation="NSESLQALRVRYLGKKGELTQMLRSLGNLPPEKRPAAGQALNQL
                     RQEIEAALDARALLIAREEDEAREATDFVDVTLPPRGRGWGGFHPVVQTMNEIIDIFV
                     GMGFSVAYGPEIEDDFHNFEALNIPPHHPARDMQDTFYLDNGRLLRTHTSPVEVRAML
                     RWGAPLRIIVPGKVYRRDSDQTHSPMFHQLEGLLIDEDVSLADLKGCLNTFVGAIFGR
                     PLDSRFRASYFPFTEPSLEMDVECFACSGRNPSCRICKGTGWLEILGCGMTHPNVLRY
                     GGIDPDRYNGFAWGMGPDRVAMLKYGLTDLRVLFEGHGSFLTGGGY"
     gene            32937..>35206
                     /gene="pheT"
                     /locus_tag="PLY83_01340"
     CDS             32937..>35206
                     /gene="pheT"
                     /locus_tag="PLY83_01340"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOV28712.1"
                     /translation="MRISWKWLNEFCKIPLSPEEAAERLTVTGCEVENIERPCSALKG
                     LVVGKIERLQIHPERPDLFVAEVDLGGPRAQCVTAARNLREGDTVPYGAPGSVIADGT
                     VLGKRTFDSVVSVGMMLSAEEIGLPGIADEFGILRLPAHLTAGEDLLKALNLDDCILE
                     ISITPNRGDLLSLMGLARECYGVIEGSTLEKHELKLPRIGDPRSISWEGVTLEDPGCP
                     LYGLSFAPSVQIGPSPMETRIRLAFSGIRSINNIVDATNYIMLVTGQPLHAFDLDRLP
                     AREITVRGAREGERFRTLDGQDHTLAEGDLLITSGGIPVALAGVMGGENSEIKAETKS
                     VAFESAFFEPLRVSQTARRLGMGTEASYRFSRGVDPSRVLPTLSCLYEYLLKDAAQHH
                     FEENVRVVSTRSWEKRRISLTRRKMHRVLLWDDLEQASKILQKLDLREISATDEKRVF
                     EIPTSRADLYIEEDLIEEVGRLRGYESVPSRIPPLLHGTGRVGEEVEAHRRIRVAAMS
                     RGYLEIVTYSFINPKWVDWLRIPEGDPRSRGFRLTNPISQEQSLMRTTLLPGLLQALQ
                     TNLRSGWKDPIRLFEVGRVFLPGEEGEDHRESLRMAGVSWNGKERRVLHETSLKDDVL
                     SIKADVMALCGSRGQIPGFSTGHEPFGHAGQTAQVSLEGKPCGYLTSIKPGILRELEI
                     PGPVFAFELEIESLLPEKGFRFGQVVRYPAVYRDISLIAPVNKPAASVEEDLRRLGGT
                     LLRELVLFDVYQGPGIP"
     assembly_gap    35207..35306
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <35307..37628
                     /locus_tag="PLY83_01345"
     CDS             <35307..37628
                     /locus_tag="PLY83_01345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HOV28713.1"
                     /translation="ELRKILQIIGKHCRSELGIKILHGVLPAQDRKQLDLRQALLRNV
                     LRYREIEGDFPWDSRIVPVTALLEEAKSSALMSGEDLVHVRNLLALATEVKNEIRRVQ
                     ERFTELEQIGRRIRDFSAELALLDVLDEEGSLYDHATPQLREIRNRIESLKATTRKTG
                     QALLNDPATSNLLQDRVLSFRNGRFVVLVRAENISSYPGIAIDRSGSGNSFYVEPYRL
                     AALNNRLSAAVEEERQEVFRILRRMTLKILERETAIAEAESALGSLDLVCAEAEMVEK
                     GGWHLPEISLSPGFRFHGLVHPLLGEKAVPIDIACGDDFRVLVVTGPNTGGKTVALKT
                     VGVAVILSWMGLPISASEGSVVGEIDSVYVDVGDEQSIEQNLSTFSAHVKNIVSILME
                     TGPGSLVLLDELGSGTDPQEGAALGIALLEAFREKGALVLATTHHNSIKTYALTTPGV
                     ETASMEFDAVSLKPTFALLMGIPGKSNALLIAERLGMPPAVIERARANLRGEDVSAEE
                     LIGQLQEKRAALEIEARNLAEERARLIAARQSLESRISRFEAAKEDRLKEADREAGKI
                     VREAQEAARELLRSLERSKTVPAAHRAINAKGRDLEKIRKEAEIREEKRIEKRSVKAE
                     GIRLQPGDTVQMVDSSIKGIVESVSGEKVTLVAGSFRMEVPMNRLKKAGEALSKTKPE
                     ISVKTIRPVGIPGSIMIRGMTVDEALPEVERYLDQAYRAGYDQVAVIHGRGTGTLRKA
                     VTELLKTLSYVKEYRLGEHGEGGYGVTIVTFSR"
     gene            37647..38336
                     /locus_tag="PLY83_01350"
     CDS             37647..38336
                     /locus_tag="PLY83_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOV28714.1"
                     /translation="MEEGFFNLKSLREQVYEYLKIQLNEGKLEHGAFLNLNAISAEMG
                     ISKTPLRDALFQLEAEGFVTIYPRRGVKVNTLTLEKIRNIYQILGGLEGAVLVEVASK
                     FRKKDVDAMRQFNEEMKDALQRNDFERFYSRNLDFHNAYLELSENTDLLRTVRILKER
                     LYEFPRSKGFLKEWEVESTGEHDRIIEFLAKGDFNGAADYIRDVHWSFAVQERYIRKY
                     YFGLAAQTAER"
     gene            complement(38408..>39405)
                     /locus_tag="PLY83_01355"
     CDS             complement(38408..>39405)
                     /locus_tag="PLY83_01355"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOV28715.1"
                     /translation="SRFFHVSACESPDELLLSRLRQFSSKALIRQSRPVMAPLKGTDL
                     FQDTWALLRLAEERGWSLGKAALANEEALLGIPREELSREMEQRLVVMLNAVDSGLSM
                     TGGMKLLRPMASSILDAESKGRLFLGGPHLRAAARAMAAMHVNASGGVVCAAPTGGSA
                     GVLPGILSTLILDFGLSKEKVLDALWAAGAVGQALDRVSTFAAEVCGCQVEIGAAGAM
                     GAAAVVEAAGGTARQACDAAATVFQNIMGSVCDLVQGIVEIPCHSRNAALASMAFLCA
                     DLVLGGYENPVPLEETLVAVDSVGRMLPEELRCTARGGLALCPSARAMPRLDVGK"
     assembly_gap    39406..39505
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<39506..39803)
                     /locus_tag="PLY83_01360"
     CDS             complement(<39506..39803)
                     /locus_tag="PLY83_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase beta chain"
                     /protein_id="HOV28716.1"
                     /translation="MSILNHVIGPIMRGPSSSHTAGPWRIGRISRDLLGSKPTMARFL
                     IHPSSSLSVCYHDQGSDLALLSGLLDIPLTDERFPRMLELAPEMGLQARFELTPF"
     gene            40156..41172
                     /gene="dctP"
                     /locus_tag="PLY83_01365"
     CDS             40156..41172
                     /gene="dctP"
                     /locus_tag="PLY83_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOV28717.1"
                     /translation="MTKTRKSLLLSALLFVSLFALMASSALAAPAVTLKFAGQSPADH
                     PASILMNEIAKEVAQKTNGRIEIKVYPANQLGDYTLVYEELIKGTIDMSCTSVPSQFD
                     PRLELVYINGFVKGYGDVKRVFAPNGWLFKKMDELNSRLGVKLGGFFIEGMIGTATTK
                     PAASPLDPKVKKGVLIRVPNMDVYKLAAEAMGYRTVTIPYADVYQALQTGVAEGVNGF
                     SVVAAYTMLRDVIKYWYMTNYSLECLNYMISGKTWAKLSPADQKILQEAINHAAAKSI
                     TLAKQSDEKYMQLMRDKGIKVYTYSEKELIPIAKACASTWPMLEKKMTKELIDEFRKE
                     LAPK"
     gene            41331..41855
                     /locus_tag="PLY83_01370"
     CDS             41331..41855
                     /locus_tag="PLY83_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HOV28718.1"
                     /translation="MMKPRNLFDKIVVNGFSAVTLVMSILLTILIALATFIRYVIQGD
                     LYGYEEWVKLFAFWLYFSGAAIGAFNRTHVSADLVQSYVPEGVLKRLLVFLKDLITVA
                     VTLLFVWYGYGFFMFGFRGPLGTGIALPRTTIWQIPLWTSYLAIFLGLIFMAFYFTRD
                     LFLSAKALFKGGRK"
     gene            41852..>42753
                     /locus_tag="PLY83_01375"
     CDS             41852..>42753
                     /locus_tag="PLY83_01375"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOV28719.1"
                     /translation="MIYVAIVILLATLVLGVPVPVSFMASAAWLLFFGGPDGAGYEAS
                     QLLPYAFAQINSVSLIAIAMFIMAGGIMERGKIGEKLIDLVDVFVGHIRGGLGIVGTI
                     SCAVFGSISGAACATLSCIGTIMFPRLEAGGYPRGHSAALMANASLLGLLIPPNATLI
                     IFAWLSGQPILACFLSTVMPGIVTIILISVVNMWLLRNNKEVLVAHKRTTAEKWELFK
                     TRGKLAIPALMLPVIVLGGIYGGIMTTTEASAMAVLYAIPVGVLVYKGMKWREVIGPL
                     VESAVTTGVIMAMLFSVSMLSRLYL"
     assembly_gap    42754..42853
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <42854..43323
                     /locus_tag="PLY83_01380"
     CDS             <42854..43323
                     /locus_tag="PLY83_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOV28720.1"
                     /translation="GVIMAMLFSVSMLSRLYLLEDLPSRLLALFRAVSENRWVILFMI
                     NVFLVIMGMLMDDISVVVLTTPIMMPIIMELGFNPIHYAAIIGVNTALGCITPPAAPV
                     LYLSGRLSKTPINEMMTPALYFMLLCWGPVLLFTSYVPKLVLFLPHIVLGTPW"
     gene            complement(43581..44198)
                     /locus_tag="PLY83_01385"
     CDS             complement(43581..44198)
                     /locus_tag="PLY83_01385"
                     /inference="COORDINATES: protein motif:HMM:NF020905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HOV28721.1"
                     /translation="MKGFLYYRKEILVPGGYARAFEIEKGDRITLIDVEGGQVCVFVA
                     FYRDRLSEFLSPAHTRLALNSVSIRGKDRLRSNLLRPLLEVTENTAPTHDFLMPACDS
                     WVGGTDDHHSCIGNFEGALAPWEISRNQVPEPLNVFGNTLFETDGTLVRLPPVSKPGD
                     RLTLTALLPLVCAVSACPREPLGPGKTRVSDISVIVSHGDEVGKT"
     gene            44325..44401
                     /locus_tag="PLY83_01390"
     tRNA            44325..44401
                     /locus_tag="PLY83_01390"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:44359..44361,aa:Arg,seq:ccg)
     gene            44492..44812
                     /locus_tag="PLY83_01395"
     CDS             44492..44812
                     /locus_tag="PLY83_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV28722.1"
                     /translation="MGNSWKKKFQELGMALEEQGNEVLERVSEEALAIGKQAKASLSR
                     ARKKTEEILNSIQEIEEIKARLDTIENDQQRLSKLVSELAQQMKAIRGESRQRTQAEE
                     GENE"
BASE COUNT         9651 a        12021 c        13448 g         9067 t
ORIGIN      
        1 cgcggatgga ccggatggcc gagatgctcg cctgttcacc cgaagagacc gtgctggacc
       61 tcggaggagg cacagggctt tttgcctgca ggctcgtgga ccgttgcggg gaaatccacc
      121 tgctggacga aagcttgagg atgatccaac agggatcttg cgggaaaatt cgcgtgcgtc
      181 agggagacgc aacggacacc gggtatccgg aacgcttctt tgattgggtc gtcctttcgg
      241 acgtcattca ccacatccgg gaacaggatc ttctttttcg ggaagtcgcc cggatcctga
      301 aacccggcgg aaaacttctg gtcaatgaga tcgaccggga gaaacctttg ggtcggcttg
      361 tcggaaagct ggagcggctt gtcttccctc cggtctatca cattggcttt aaggaacttt
      421 ccaattccct tcttcgctac ggtttcaccc tcaggcaaca ggttcaggac gcctggagtt
      481 tcatcgcatt atgggacaac tccgggggct aatctttggc gctgtggtgg cgtctcgaca
      541 attcccccag gatgtccgtg ggcttgagat ccagggaaat gaaagcgacg agcaggtggt
      601 acaagagatc cgcggcttcg tagcgagtgc cttcccgatc cttcaaagca acggccagag
      661 cggtttcgac gccttcctcc cccaccttct gcgcgattcg ggaagatcct tcctgaagca
      721 ggcgggccgt gtagctctct tccgcggggg cgtggcgcct ttccagaagc catctccaca
      781 gccgccccca gaaagtgcaa acggtcccgc cctcacccca gagagacctg tagaagcagg
      841 tcctctggcc ggtgtgacag gctggacctg caggctcgac gagagcgagc agggaatcgc
      901 cgtcgcagtc aattttcatc tccacgaccc gcatggtgtt gccgctcgtt tccccctttt
      961 tccagaggcg gttccgggaa cggctgaaaa gggtcaattc tcccgatcgc tccgtttcct
     1021 gaagggcctc cctgttggtc caggccacca tgagcacttc gcccgattcg caatcctgaa
     1081 cgacaacggg caccaggccg gccgaatcga aacggatctg atccagaagg ctcatcttga
     1141 ctttccctcc tcgcttccga ttcgaacggg aacgccccat tttgccagtt ccagcttgac
     1201 ttcccggatc gagagaaccc catagtgaaa gatcgacgcg gccaaagctg catccgcccc
     1261 ggcttcgaag gcgaggcgca ggtcttcggc cgaccccgct ccacccgagg cgatcagggg
     1321 aacgggagcg gtttcggaca cggtctgaat cagctcaaga tcgtacccct cgtttgtccc
     1381 gtcctggtcc atgctggtca ggaggatttc cccgcacccg aggcgcaccc cttcccgggc
     1441 ccagtctaaa acatcgaggc ccgtggaggt tcggccccct tcgacaagaa cctcccagcc
     1501 cccatcgggc ttccgtttgg catcgatcgc aaggacgacc gcctgcctgc cgagggttcg
     1561 ggcgcaatcc cggatcaggg ttggattgcg gacggcccca gtattcagcg acaccttgtc
     1621 agccccaagt gcaacaaggt gtcgtgcctc gtccgcatgg gtgatcccac ccccgacggt
     1681 aaaggggatg aagagccgtt ccgccacagc cttgacccag tcggtcatcg tcctgcgtct
     1741 ttccactgag gccgaaatat ccagaaaaac gagctcatcc gcgccttctt ccatgtaggc
     1801 tgccgcaagc tccgcgggat ctccggcatc ccggaggttc cggaaacgga tccccttcac
     1861 gacgcgtcca cccttgatat cgaggcaagg gatgattctt ttcatcagca ttgtcgttcc
     1921 acctccaggg cgtcttccag cttcaggtcc ccctcgtaca gggccttccc gatgaccaca
     1981 tcggaaatcc cgagttcggc aagcgcctgg atgtccttca ggcagcggat gccacccgcc
     2041 gcggtgaatt gcataccggg gaaggcctcc cgcagagtgg agtagagtcc gagatcgggt
     2101 ccctctcctg ttccgtcccg gtcaaccgcg gtgacaaggc aatgccggaa accggccatg
     2161 aacagccgat gcagggcttc ttccgggccc agggccgcct tttcggtcca cccgctaacg
     2221 gcgacttgtc ccttcttgat gtcgatcgtg gggatgacgc gttccccgaa tttccggaaa
     2281 agatcaaaag gcgtttccct ggtggcgaaa agaaggctgc ccaccatcgc aaaagcggct
     2341 cccatctcga gaaccctccc gacggaagct ggggtcctca ggccgccacc gtaacggatg
     2401 gtcaggttca gggcggaaag cggtgccagg atgtccgcat ggctgggggt tcccctctgg
     2461 gccccctcca ggtcgaccac gtgcagccgg gggcatccac aggacttgaa ggcggaagcc
     2521 acggaaaggg ggtcatcggc atattccgtt ctggcggaat agtctccccg ggtgaggcgg
     2581 atcaccttcc cctggtaaag atcgatcgcg ggccagattc tcatgaggat ttacccgaaa
     2641 ggaaacggat gaacgtccca aaaagctgca acccttccct gccgcttcgt tccgggtgga
     2701 actggaagcc tgcgattttg ccctgcaggg agaccgagct gaaacgtatt ccgtcgatag
     2761 ctgtgtcggc aaggcttgcc tcgttgacgg gggcggcgta gccgtggaca aagtaggcat
     2821 cgagcccccc ctggaaaaga tcggtgatcc cgggcagctc cctgagccag gaaactcggt
     2881 tccagcccat gtgaggcgtt tttttgagac ctgcgagggg agcaatcgtc cctgggaaga
     2941 aaccgaatcc ttccgtaagc ccgtcttcca ggcttgcttc gtaaagaagc tgcattccca
     3001 ggcagattcc gagcaggggc ttcccggtgt ttccccattc cctgaggacg ggaatccatc
     3061 cccttctggc gagttcctcc cgtgcaggag ggaacgctcc gactcccggg aggacaagca
     3121 aatcgacctg atcgcccagg tttgagggac ttcccagaag ggaggcctca tattgtaggc
     3181 ttgaaagggc ccgcaaaacg ttccccaggt ttcctgcccc gtagtcgatg atcccgatca
     3241 tatccagagc cctttcgtgc tggcttcacg gcttgaaggg acgagagctg ccctgagggc
     3301 ccgcccggtg cctttgaaga tcgcctccgc aaggtgatgg gagttatcga ccgcaagggc
     3361 atggatatgg agagtcactt ttccttcccg ggcgaaggct gtccagaatt ctcggaccag
     3421 ttccgtgtcg aattctccgc agcgttgtgt gggaaagacg gcattccagg tgagcccacc
     3481 ccgtccgctg atgtccgctg ccaccagaac aagcgtgccg tccatgggga gggcgatcga
     3541 ggaatagcgt tcgcagggac cgcttcggaa aagatccagg aaagccgacc ccatcacgat
     3601 ccccacatcc tcggtcaggt gatgggcgtc cacttcggta tcgcccgaag ccttcagttt
     3661 tccggggaca tcggcatggt ggagaaaaag cccgagcata tggtttagaa acccgatggg
     3721 agtttccacg cggaccgttt cccctggggg aaggaactcg aactccacag ccgtttctct
     3781 ggaacgcctc tcataccgct tttccatcct catcgcccgc tttcttcgaa gaaccggaga
     3841 atttttcgat gatctcctcg atgccattcc aggccagctg caggctcttg cagctagcca
     3901 cgaacttcaa actgactgga gcgatttcct cgatcacgga aagcgcattg gctttaaggg
     3961 tcgagccggt ctgaacaata tcgacgatgc aatcgctcag ccccaggcgc ggagccattt
     4021 cgatcgaacc gttgaggtga acgaactcga cctggattcc ccggtccgcg aaaaaggctt
     4081 ccgcgattcg ggggtatttg gtcgcgaccc ggagccccat cagccggctc tcatggccga
     4141 ggaaccgagc ggcaactttt ttggggcccg caaggacgat ccggcaaagc cctcttcccg
     4201 tgtccgcaag ttccacgagg gaggctccgg tttcccagat cacgtccgaa cccgcaaggg
     4261 caagatcggc gatcccgtaa tggacataga cggggacatc cattggcttt gccaggagga
     4321 aacggaaatc gccttcctgg atgaccaggg atcggcctct atccctcagc ctttgagttg
     4381 ggatatcgag attttcaaga acctcgaccg cctcgtccag gacccggccg gtcggaaggc
     4441 aaagcgtcag catccggact cctccccttc cattgaatcc agcgggtagg cggtgcaagc
     4501 cggtagatcg atccaccagc tgtaatttct tgaacgggcg agctggatcg ccccctctct
     4561 gttctcttgc cagctcagct cgatcctgta ccccgaggcg atgaaatcat ccgcccgctc
     4621 gagggcgact ctcggggaaa gctccccgca ccatgcgact gccggcgttg ggccctcaaa
     4681 gcgggagagc gaggcgaccg ttcgcaggtc cagcccaaac ccgagggcct gcccaacaat
     4741 gccgtactgc tccagcaggc cgtcgtacct tcccccgcct ccgaggctga acccggcact
     4801 cccggaaaaa atctcgaaaa ccgggccgct gtagtaccca agttctcggg ccagggcgag
     4861 gtcgagcctc aggttttctc ccagcccgag ttcttccagc gcttcgacga tcagggagag
     4921 gtcccgcaag ggctgcgttt ctccaaaaag gcgcgtggct tccttcaaga cccgggaagc
     4981 gtttccccgt aaatacggaa gctgaaaaag cggatcttcg gtcccgctcg gtagatgggc
     5041 ctccccaagg gcgttgaaga aggcttcgta acggcgttca agaagggcca gcgtcaacag
     5101 ggacgagaga gtccgatcga gacgcgaaag ggactcccgg agaaaacccg cgtgccccag
     5161 gacgacgacg ggagaatgaa tcccgagccg tcgcagggat ctcaagagaa gcgcgatgac
     5221 ctcgacatcc gccccttgtc cttcccatcc gagaagctcc gctccgaact gcattttttc
     5281 gaaatcgttt tctggcggga tcggagccct gtagaggggc cccgcgtaac agacccgcag
     5341 cggcctttcc tgaggagaaa agtgcgacga gaggtaggcg actgcagcca gggtcaagtc
     5401 cgccctcaag aagcagggtt caccggaagg ggtgtagacg gggatcaggc gtttcctgaa
     5461 gcctggcggg agcttttccc acgcggattc aaaaagctgg agccccggag cgtaaaaagg
     5521 ttggtatcca taggcggaga aggtttcgaa gaagaccttc ctgcagcgtt cgaccctttc
     5581 tccctcccgg cctccgagcg tccgtgtccc cttgggttga cggctcaatt cccgggtcct
     5641 cccctcagcc gcattgcgag ggcatgggcg tgcagccctt cggcgagagc caggcattct
     5701 cccggctttg acagcgaagc ggccgcctct ttcgtcattt ccaggtgggt caggggcctc
     5761 aggaaagttc caacccagag gccgccggag tagcgggcgc gcccctccgt ggggagaaca
     5821 tggttggtgc cggcgatgta gtctccgaac gcttccgctg aatgatggcc aaggaaggcg
     5881 gccccatagg ccgtgcattt ctccaggacc tcccgcggat tttgaaccgc gagttcgagg
     5941 tgttccgggg caacccgatt tgcggcgtta acggcttcct cgagggtgca gaaggcgacc
     6001 gttcctgaag cctcccaggc ctgttgcgcc gtctttgacg tgggcagcgt ctgcaggagg
     6061 tcctcgactg ccgccacggt tctcttggcc aggctttcct caaggcaata cagggaaacc
     6121 cgggccagcg ggtcgtgctc ggcctgggcc agcaggtcgg aggcaaggac gcagggattt
     6181 gcggtgtgat cagcgatcac caaaacctcg cttggtccgg ccaggctatc gattccgact
     6241 ctcccgaaca tctgtctttt cgcttccgtt acataggcat tcccgggtcc tgccagaaga
     6301 tcgaccgggg gaatccccgg aagccccctg accaaggctc cgactccctg ggctcctccc
     6361 accgcgagga cctctttcaa accgagaagg cacagtgtgg ccaggatcgg cttggagatg
     6421 cccgagctgc ctcttggggg agagaaagcg acaattcggg aaacaccggc ttcctgggcc
     6481 gggatgatcc ccatcacggc cgtgctgatc agcggatagc ggcccccggg gatgtaaacc
     6541 cccgcgcttc tgacggggag gaaccgcatg ccggccctga tgcccggcga gatttcccat
     6601 tcctggttcc tgaacatctc ccgttgtgcc ctgtgaaagg cacggacgtg atcgatggcg
     6661 gtttcgaccg cctgcctcag tgacgggtcc atttgggcca gggcctcgtt catggctttt
     6721 tcccgaacca ggatcgggcc ttcgtagcca tccaagtggc ggttgagttc taacagggcc
     6781 tgttccccat cctcgaaaac ccgttggagg atcgaatgaa cccgttcctg gatggaacgg
     6841 tcgatggact cgcggtcaga agatgccggg gccttgacga tgatcataca tgtctccttt
     6901 acttatttag tataatagca tgataagaat tggcgcggat tgcatcatat cacgctaccc
     6961 tggctcctgt aaagggtctt tgaaaaagga atcccgacta gcaattggaa ggcctcaagg
     7021 tcgacaaaca cgacgcgagt tgttaaaatc tatcgtgtta aattgtagaa gtctggaggc
     7081 aaaaagaaac gaaatatggc gagaaaaccc ggggaaaaac gctttcgttg ctcggaatgc
     7141 ggtttttttg ctctgacctg gtcaggaaaa tgtcctgcct gtggaagttg gggatcattt
     7201 gaggaagaac gggccgaatt cacgtcttcg gaccttgcct cgaaaaccgt gacggccgtc
     7261 tccggcctct tcgaccttgc attgcccggg agaattccct ccgggataga agaattggac
     7321 cgggtgacag gcgggggctg ggtcgagggg agcgtggccc tgctcgcagg tgagccgggg
     7381 atcggaaagt ccaccttgct gctgcaaacc tgcgccgcca tttcaagggc cggctaccgt
     7441 gtcctttatg tttccggcga agagtccgcc ccgcaggtcg ccttgagggc gaggcgtctc
     7501 ggtgcggcag attctggagt ctcggtcctt tgccaggaaa gcgttcagga aattctaccg
     7561 ttgcttcaga agtacgattt cgcggtgttt gacagtgttc aggcccttcg tcatgcggac
     7621 acgccgggtt ggccggggac gccgggacag gttcgagccg tcgcgcaggt gatctcggag
     7681 gaagccaaaa gaagcggctg ctctgcggtt cttgtggggc acattacaaa agacggccag
     7741 atcgcgggcc caaagcttct cgaacacatg gttgatgtcg tcctttactt taccggtgac
     7801 aggacttctc cctaccggca tgtccgggga gtgaaaaatc gttacggcag tacggatgaa
     7861 ttaggcgtct tcgagatggc ggaagcgggc ctggtgggaa ttccggatcc tagccggctt
     7921 tactggaacg aaaacgagga gcccatcccg ggtacggctg tgacggtcgt cctggaaggg
     7981 gctcgtccct tggtggcgga gatccagtcg ctttcttgca ccactccgtt tccgtatcca
     8041 aagaggacgg cgcggggggt gtccatcagc cggatgggac tgcttttggc ggttctggaa
     8101 cgaagggggg gattgccatc gcgatcgatg gatgtcttca tgaatgttgt ggggggactt
     8161 catatcgagg acccggccgc agacctggcc gtctgcatgt ccctggcttc ttcccttact
     8221 gaccttcctt tgccccgcga cacggtgttc atcggcgaag tgggattggg aggggaagtc
     8281 cgtcccgtac ctcgtatcgc cacgcggatc agggaatcgg cccggctatg cttttccacg
     8341 gttgtcacga gccgttacca ggatcaggac gtcccggcag ggatccgaat cgtaaaagtg
     8401 ggatcacttg gcgaggcaat gaaggagttg accgggggat gaaaaggttg atcaaagcgg
     8461 ccttggccgt ttttctcgtt gtttttcccc tctggggaat cgcagaaagt gcgcccctgt
     8521 cgtcagaaaa gacggcggtg gtcgtttccg tgccgatgga gggggtggtg ggggtttccc
     8581 ttgaaaatta cctggaagag gcgttcgcct atgccgtgaa ggaaggagcg gccctggtag
     8641 tgatcagcct ggataccccc ggaggcctgg tgacctcgat gcgcggaatg tcccagaaaa
     8701 tcctttctgc accgattcct gtggcggttt gggtgggtcc aaccggggcc cgggcggcat
     8761 cggccggagc gtttcttgtg atggccgcac atatcgcggc catggcaccg gggacaaata
     8821 tcggggcggc acaccccgtc agtgcttccg ggcaggatgt gccggacagc gaaatgaacc
     8881 ggaaaatcac caatgacctt gctgcccaga tgcgatccct tgccgaggaa cgcgggagga
     8941 actccggagt ggccgcatcg atggttctta acagcgtttc tctgaccgcc agggaagccc
     9001 tggaaaagaa ggtgatcgac acgatcgccc ccgatgtgcc ttctctgctt aaatgggccg
     9061 accaccgcaa ggtaagggtg aaaacagccg acgtcgtcct ggatctccgg cgctacgaga
     9121 tccggacctt cgagatgagc gcacggcttc gggcccttca cttcgtttct cggccggatg
     9181 tggcctacct gcttctgacg ctcggcatcc tggcggtcgt tttcgaggtc ctgacaccgg
     9241 gagggttcgt cctggggacc gcaggagccg tgatggcact catcggcgcg tacggcctga
     9301 ggatgcttcc gttcaactgg gccgggatca tccttctggc cgccggaatc gtcgttctcg
     9361 tcctggacct cgtcgttggg ggaattggtg tcctgagcct tttcgggctg gcttccctga
     9421 tcgtggggtc gctggtactt ttcagggcac ccgggggtga gctcttgaac gtgtcgttcg
     9481 gattcatggc cggggtcgcc ctgaccctcg gtcttttctt tatcctggca gcgggagctg
     9541 tttggcgttc aacaaggaag agggtaactt cagggcagga aggaatggtc catgatgaag
     9601 gcgttgtcgt cgaagacctg gccccttacg ggtttgtgag ctgtcacgga gaagtctgga
     9661 aggccgtttc ggaagacggg gcaagcatcc ccaagggaac ccgggtccgg gtttcccgag
     9721 cagaaggcct gttgctccac gtcagggaag aaagagcgga atccttgaaa gagaagggag
     9781 cggagcacaa atgattggta gcctgatcga ttttcttttc aacgcgggaa gcacgctcgg
     9841 aatccttttg atcgcgctgg tccttctgac ttccgcggtc aagatcattc cggaatacca
     9901 aagaggcgtc gttttccgcc tcggacggct tgtaggggcg aaggggccgg gtctcgttct
     9961 cattgtgccc tttgttgacc ggatggttcg gaccgacctc agggttgtta cgctggacgt
    10021 tccggtccag gaagtgatca cgaaggataa cgtcccgatc aaggtcaacg cggtcgtcta
    10081 tttcagagtc atggatccca accgttcggt ggtcgaggtc gagaactaca tcatggcgac
    10141 cagccagctt tcccagacga cccttcggtc ggtgatcggg cgggcggaac tggacgaagt
    10201 gctgagcgaa agggacaaga tcaaccttga gcttcagaag atcatcgatg aaaagaccga
    10261 cccctggggg atcaaggtga gcgcggtcga agtcaaggag ctggagctgc cagaggggat
    10321 gaagagagcc atggcgcgcc aggcggaggc cgagagggaa cggagggcga agatcatcag
    10381 cgccgaggga gaggcccagg cagccgaaag gctcagcgag gccgcccgga tcatggccgt
    10441 gagtccgatt accttgcagc ttcggtacct gcagaccatg agagagattg ccagcgagaa
    10501 gaactcgatg acgcttttcc ccttcccctt tgaaatcgtg aaagcgttca tggaacgggc
    10561 caacccggaa aagggtcctc tcggctaggc gggaaagttg cagaggcgca ggccagaaag
    10621 ggcctgcgcc tctgcttgtt ttgcctacgc ttgccatggt gggtataata aaccaacatc
    10681 cggtttggat ggtttaacgg gaggggagag gcctagatgc catatgattt tgaacgcgtt
    10741 gagaaaaagt ggcagcaata ttgggcagaa aacagggttt tcgacgtcga acgggatccc
    10801 gggaaaccca aattctattg cctggagatg tttccctacc ccagcggtgc cctgcatatg
    10861 gggcacctga ggaactattc gatcggcgac ctcctgagcc gcttcctctg gatgaaaggg
    10921 tggaatgttc tgcacccgat gggctttgat gcctttgggc tccctgcgga gaacgctgcc
    10981 ctgaaatacg gtgtccaccc caagaagtgg acctgggaca acatcgagca catgaccgcc
    11041 cagctcaaat cgatgggatg cagctatgac tggagacgaa gggtcgaaac gtgtgccccc
    11101 gagtactacc ggtggaccca gtggattttc cttctcttcc tgaaaaacgg ccttgcctac
    11161 cgcaaacacg cgccggtcaa ctggtgcgag agctgccaga ccgttctggc gaacgagcag
    11221 gtgattgacg gaggacattg ctggcgatgc ggcagtgtcg tcacgaaaag gaaactggag
    11281 caatggttcc tcaatatcac ccgttacgcc caggaactgc tcgattcgct ggataaccta
    11341 ccgggctggc ccgaaagggt caagctcatg cagcgcaact ggatcggccg atcggaaggc
    11401 gtccgcctga ctttccctgt ggaaggaatg gaaaagggcc ttgaaacctt taccacccgc
    11461 ttcgacacga tctatggggt cacattcctg gccctagccc ccgagcatcc gatggtggag
    11521 gagtttgcgc ggttgagccc aaacggggaa caaatccggg cgttcgtgaa ggaaacgatt
    11581 cagaagagcg agatcgaacg gacggccgtg ggaggggaaa agaagggcgt cgacacgggc
    11641 ttcaaggcga tcaaccccgt caatggagaa agggttccca tctgggtttc caactatatc
    11701 ctgattgaat acggaaccgg ggcaatcatg ggcgttccgg cccatgacca gcgggacttc
    11761 gagtttgccc gcaaatacgg gatcccgata cgcctcgtca tccagcctga agcaggtgcg
    11821 ctcgatcctg aaacactgtc ccatgccttc gaagaaggcg ggatccagtg caattcggct
    11881 tccttcgacg gcctcccgac gcgggaaagt atcccccgga tggcccagtg ggcggaggaa
    11941 accggcatcg ggaagcggga ggtcaattac aggcttcgcg actggctcat ttccaggcag
    12001 cgctactggg gagcgccgat ccccgtggtt tattgtgaaa agtgcggtat cgtccccatc
    12061 ccggaagagc aactgccggt tcttctcccg gatgtccagg ttcttcccgg ggggaaaagc
    12121 cccctgccgg aaacccccga gtgggtcaat acgacctgcc cggcctgtgg tggtcctgca
    12181 aagagggaga ccgacaccat ggacaccttt atctgctctt cgtggtattt cctccggttc
    12241 acctctccat ggacgcaaga cggccccttc aggggagagg acgtcaacta ctggatgccg
    12301 gttgaccagt acatcggagg catcgagcac gcctgcctgc acctgattta tgcgcgcttt
    12361 ttcacgaagg tgctttcgga tctggggtat gtagaggttc gagaaccgtt cacgaacctt
    12421 ttgacccagg gcatggtcat caaggacgga tccaagatgt ccaagtcgaa ggggaatgtt
    12481 gtggacccgg acgagatcat tcgcaagtac ggcgctgaca ccgctcgcct tttcatcctt
    12541 tttgcggctc ctcccgacaa ggacttggac tggtcggaga agggggtcga aggaactcac
    12601 cgttttctca aacgggtctg gcgactggtg gaagagggag gggcccgttt gaaggaggcc
    12661 ccgcgtcgca ggatcccgct ggctgaaatt gaggaaagga gccgtcgcga tctcaagcgg
    12721 aggatccata gcacgatcga aaacgtgggc agggatatcg agaaggagcg gcagttcaac
    12781 acggctgtcg cacggctgat ggaactgagc aacgatcttt cggactttgt cccggaaacg
    12841 gaccaggact ggatcctgta ccgggagggc atcgaaaccc ttttgctttg cctttctccc
    12901 ttcacgcccc atctttgcga ggagctctgg gaaatgctgg ggaacaggga ctgtttggcg
    12961 aaggccgcat ggcccgaggt cgaggccgat tgcctggagg cggagtccgt cacgatcgtg
    13021 atgcaggtga acggcaaggt gagagagaag atcgagcttc cggcctctct ttcggaggaa
    13081 cagctgaagg aaagggtcct ttcccacgaa gcggtccgga aacggctgga agggaaggag
    13141 attttgaaga tcatcgccgt gccggaccgg ttggtcaatg tggtggtgaa gggctagtgc
    13201 cccacctgat catagcgggc gccgctggaa cgacccagcg gcgcctggtt tcagagacgg
    13261 tgcgacggga gcgggagatc gggtatcctc tcgcagcgag aagggaaggg ggggactgga
    13321 gggaactcct ctccgcggaa aactgcggtg gactcttcga cgcccggagg cttgtgcttg
    13381 tcgaagaggc tcagcagctg gggaagttcc cgatcgagct ggaatccctt ctcgagaatg
    13441 cctcttctgc cacgcacctc cttttggtct acgacgaaga tccgaaggcg gtattcccga
    13501 aggaaaccca ttcaaggatc acctggctca agggagaaga aatcccgcgg tttggggcgg
    13561 caagaaggcg atgggccgaa gagagggcct cggaaatggg ggtcctcctt tcggaagatg
    13621 ccatgggcct tcttctcgag tcggtggagg atcccgaaga actcgtttcg gaacttgcga
    13681 aactggctgt cgcggcagcc ggccgcaggg tcgaagtcga agaaatccgg gctctttgct
    13741 ttgatgaggg ggaaaaggcg cttctttccc tgctggatgg tttgtgcgaa gcggacaggg
    13801 ggaaggttac gacagtcctg ccgcatgtga agaggaggga gctgatcccg gtccttgcgg
    13861 ccttgcacaa tcgtttccgc ctggccttct accgttcggt cttcaaggct ccccgtcttt
    13921 tgaaaaccgt cagcgaggcg ctgaaagcga ggccctacca ggaaagacag gcagccgaag
    13981 ccactcgcca ctatagccgg gagaagatgg cccttttcct gaaacagctc tgcgggatca
    14041 acatggggga aaagacaggg aaccgaaacg ggtggatcga actggagctg gcgattctgg
    14101 gccttcttgc ggactgcaag ccagcggaga gaaaggaaag gcgatgaaag ccgacgccta
    14161 ggcgtcggct ttcatcgctt cttttgcttt cagggtcaga gcggacttgc gtcttgcaac
    14221 cgtattggga tggaggacac ccttcccggc ggctttgtcc aacaccgact gagcttcgtc
    14281 cagacgggcc ttgatgatct gtgggtcctt gccctcaagg ctttccagaa gcctcttcgc
    14341 ggcggttttg caacgactct tccagaaccg gttgtagagt cggtttcttt ccgaaattcg
    14401 gactctcttt tcagcggact tcttgttcgg cactcggttc acctcctccc gtaaagcaaa
    14461 gcaaattcta gcatgcccaa gaccaccggg caaggagaag aaaaaacagt ccccgtgaag
    14521 ccgtattata caataccgga aggtttttga cgcggagaag aggcgggatc gatggaaatg
    14581 tcgcatctgg gccaagggat aaagggcgga aggggacttg tgacagccgg gctggtgatt
    14641 ctttccgtcg cgggcttttt tgcggaaaac cgagggattg ctctggggaa gctgctctgg
    14701 cttgccgggg ggtccgggat cgtggggctc atgacgaacg tccttgcggt aaggatgatt
    14761 ttccataagg tctgcctgcg ctttggcgcg tggcaatgga ttctgcctgg aagcggcctg
    14821 gtgacgagaa accaggaaaa aatcctggaa acgcttgccc gtgagctgag ggagaggctt
    14881 ttgactcagg aaacactcga acgggcggtt tcagaaggga agctcctgga aaagaccggg
    14941 cccactttcc gaaaagaggt ccggcggatt ttccttagaa agtcgaacct gcggaagatg
    15001 gcaaccgcag ttgaggggcc gctccagcgg ctgctgctgg ggcaggaaac cgagagattt
    15061 gtccgcctgc aggttgaaaa agcgcttcag gacagcctgc ttctcggcat ggccggaaag
    15121 ctggggctga taaagagcga gaaagttgcc gaatggatcc ttcgctctgc ttcggaaagc
    15181 ctgaaccggt cccttgtgac tgaagcgggg ttgcgggcga cacaaaggaa actggctttt
    15241 ttgctggaaa acaccgcccg gctgggggac gcggaaattc tcgagatggt caaagatagc
    15301 ctggagaggc ttctaatccg gatgctcaga gagatcgatc tcggcgaact cgcgaaaaga
    15361 tccttgaggc aagcgactcc ggagcagctg gaggattacc tggaagcgct ggccagaaaa
    15421 tacctcgact ggatcgaaat ctggggaggg ctcatgggag ccgccctcgg cctggtcctg
    15481 tgggttttgt caaaatgacc tctcacccga tggaagcgta tttccgggag gacggaatcc
    15541 tctcgcggat gcagggcttc gagttccgtc cgcaacagct caagatcgcc gtaaaagtct
    15601 gggacctgct tcagtcgggg aacgcggaca cgctggccat cgaggctccc acaggcattg
    15661 ggaaaagttt tgccctcctt gtcccgtccc tgctatgggc aacggaagcc gatcggacgg
    15721 tgcttttgct gacggcgggg cacgctctgc agggacagct gatgacgaaa gacctccccc
    15781 tcctggaagg gtttctcaga cacccctttc ggttcggaac actgaaaggg cggaacaatt
    15841 acctttgcct gagaaggtgc gccgaacttg aaaggggtgg attcctttcc ttcggggatg
    15901 agggggagat ttcagagaaa gtccttgaat ggagcaggga aagcacaaca ggggacctgt
    15961 cagaacttga actcccggaa acccatccgg ttctcgaaag gatcgcggca acccgggagg
    16021 ggtgcctggg aagactttgc ccgctgaagg gggagtgctt tctccagcgt ctttatcttc
    16081 aggcccaaga ctggcacctg atcgttacca attaccacct tttcttttca cacctggacg
    16141 ggggaaaaag gttctttccc gtccatttca atgccctgct ttgcgatgag gcacaccgca
    16201 ttcccgaagc cgcacgctca tccgcaacga gggccgcatc gccggaacgg ttccgtaaga
    16261 tgttttccgc aaggcccctt tccaacctcg tcaaagcttt ccggttcctg aaactgccgg
    16321 acgggaaatt ggaatcgggg tggcagcagg cggcccaggg ggtggaaggc gtcttcaacc
    16381 ggcttgcctt gctgtcaagg gaagagggcg tgtttacaag gcccttgacc gacctccgcc
    16441 acgacctgga aagcagcgtg gagtcccttg cgacggaagt cgaaaggttt cttgccagga
    16501 tgatggaaga aggggaggcc ccggacgttg cggaaggctt tttggtcgcc tcgcagctgg
    16561 ttgaggccct cgacgatctt cgatggtgcc ttgatgttgt ggactaccca cgctgggcaa
    16621 cctggtggga cggcgcccag atccagagca cccccgtact ggcggctgcc cctgttcgag
    16681 aggccctttc ttcgttggac gatgtcccca tcgtctgtac gtctgcaacg ttgacggttg
    16741 ggggcgattt tggcttttgg gtaagggaaa gcgggatttc ccctgcggaa accctcaccc
    16801 tggattctcc cttcgatctg ccttcgcaga tgtcgatctg ggtgatcgac cttgggcaga
    16861 gggtgacgga caaggggtac gattcaacgg caagccgggt gattgaaaag ctttgcgatt
    16921 tgaacggggg aaggaccctc gtcctatgca gctcgatgcg tctgatgagg gctgtctcgg
    16981 cgaggctgaa gtcgaaagag agagggtacc gccttctctg tcagggggaa gccccaaaag
    17041 aggaactgct tgaggacttc cgcagcgatg aaacctcggt tcttgtcgga agcctctctt
    17101 ttcgggaagg gatcgatatc ccgggagacg gcctgacgca ggtgatcctg gatcgcatcc
    17161 ccttccccca tcctgcggat cccctcgtga gggccaggcg tgatctggag ggcaaggaca
    17221 cgtttctgcg aagcactctc ccgatgagcc gcctttttct caggcaagcc tttggtcgcc
    17281 tcatacgaag gaagaccgat gcgggaaggg tagcgatcct ggatgggaga gccctccagc
    17341 gcgatgattg gggtattttc caggactttc ggggagtgcc ggtcaaaaga gtccgcctgg
    17401 ttcagactcc ttgatagcct tccgtagcct gtgttatagt atcaaggctg ccaaacgagt
    17461 ctatttaatc ttcttaagat ttttccatag caagggggtg tcgtcaatga agcggacttt
    17521 tcaaccccat aaaacgcgcc gcaagcggac aatgggcttt ctcgtgcgtt ccagttctcc
    17581 gactgggcgc cgcgtcatcc gcaacaggag acgcaagggg cgcaagcgcc tcgcagttta
    17641 gatggatcgt cttttcgggt ttccagccga ctttcggttg aaaaagggat gggaatttga
    17701 tcgggtcttc cgcaccggcc gacgcgtcaa aggagagctg gtgcggttgc tgtttctgga
    17761 cacgggtgaa gggcctcctc gactgggggc ggctgtagga aagcggcagg gggcttccgt
    17821 ggtccgcaac cgcggaagga gaatccttcg cgaagccttc cgaaactgcc gaccctggct
    17881 gaaagaagga atctggatcg ttgccatgct gagatccgcg ggactttgcg ctggagcagg
    17941 ggaaatctac gaggatctgg tccgcctcct tgagagggag ggccttttgc gtcccggatt
    18001 tcccacccac ttcccgccga acggcaaaac gaacgatgaa gagatcggtg ccggcggcgc
    18061 tagctcttag cctgatccgg ttttatcaac gtttcctttc tcctctgctg gggaagaact
    18121 gccgtttttt accgacctgc tcggcctata cgtttgaggc catcgaacgc tatggtttcc
    18181 tgcgcggggt ctacctcgga atccgtcgca tccttcgatg tggtccatgg tgtgcggggg
    18241 ggtacgatcc cgttcccgac ctggaaaagg aaaagcgcgc caaagaattc gaagattgaa
    18301 ccagagggac ggtgaaccgt ttgggtactt tgtggaacca ggcaggcgaa ctgctgctga
    18361 agatcttgaa cctgttttac gcgctgaccc attcctacgg aattgcaatt atcctgctca
    18421 cgatcctcgt gcgggttgtc cttcaccccc tgaaccagaa acagctggtc agcatgcaga
    18481 aaatgcagaa gatccagcca aggatcaaaa ccctgcagga gaagtacgga cacgacaagc
    18541 agaaactgaa cgaagaaacg atgaagcttt ataaggagaa tcaggtgaac ccagccgcgg
    18601 gctgcctgcc tctccttgtc caattgccca ttctgatttt gcttttcagg gttctcacga
    18661 acttcaagtt cgggggagtg tcgttcctgg gagtgggact tgaggaatcc gttctctcca
    18721 cgatggggaa ggctgtcggg ttaaccggcc agaccccagg catcacggcg cttctgaccg
    18781 ggattgctgc aaacccctca gggcttctga atgtagggat gtatgccccc aacctgattc
    18841 ttctcgtgtc catcgggttt ttgacgtggt tccagcagaa actttccgct tccaacaacc
    18901 cccaaatggc cttcatgaac tggttcatgc ctgttttttt gactttcatc tgcctgaacc
    18961 tgccgggagg ggttctgctc tactggggag tttcctcgct gttgagcgtt gtccagcagt
    19021 ggcgagtcgt gaaggtcacc gagctcgaaa tggagaaaaa accggttctg ttcaagaaca
    19081 agcccactcg ggacggggat tcgtgatcgg tttcccgaac gacggaaagg aaagaaaaaa
    19141 tggaacggac cgaaccactg gttgtcgaaa cggattcgat cgagaatgcc gtactcgaag
    19201 ctgcccaaaa ctggaagatc ctgccggagg atgtgcaagt tcgcatcctc gaggagggga
    19261 agcggtttct cggctttttt gcaagaaaaa ccagggtgga actgttccct tccggagacc
    19321 tggaactcct gaaagcgaaa cgcattctct ccgggatcct ggagcaaatg gggatggatg
    19381 ttgagatcca cttgggcgag gaggaattgc tgaacatcac ggggacggac gcaggaatcg
    19441 tcatcggcag gtacggcgaa acgctgaaag ccctggagta cctggtgaac ctgatttcgc
    19501 gcgagggagg accgtcccgg ccgatccggc tcgacagtga cgggtacagg gaaaggcgcg
    19561 aggaaaacct gaaacgaatg gctcttgcct ccgcaagaga ggccatcagg cgccggcgtc
    19621 cggtgacgat ggagcccatg acgagctggg agagacggat tgtccatctc gccctcaaag
    19681 acaccctgga ggtcgatacc cgctcggtgg gggacgagcc gcagcggcga gtcgtaattt
    19741 ggcccaggcc aggttcaagg cagaaaaaca ggtcgagctg aaacgatgcg ggacctctcg
    19801 ggcgggaggt cccgcatcgt ttcagtaaaa aaaccgatcc ctaacgcaac ctgcttcgct
    19861 aaaatggagc aacgaaccgg aaaggagagg tgaatcccta tggaacgaat cagaaagatc
    19921 ggtttggtct tgggcattgc actcctgttc atcggcattg tctacacggc gccacaatcc
    19981 tttaaacccg tcgtgactcc ggccgtttcg caggatgtct tcagtggaaa tcccgttgcg
    20041 gcgattgcca gcaaatcttc acccgccgtg gtgaacattg acacgaaaac gctggtcagg
    20101 cgctcttttt cgcccttcgg ggacgatccg atcctcaggc agtttttcgg agaccagatg
    20161 gacctgtttt ccagggtggt gccaatggaa gggaagggtt ccggtttcat cgtgagcccg
    20221 gacgggatga tcttgacgaa caaccacgtt gttgacgggg ccgactcggt gacggtaacc
    20281 ctggcggacg gaagacgctt tgatgcagaa atcgtcggca aggacccgac ctatgacctg
    20341 gccgtgatcc gaatcaaggc ttccaacctt ccggttttga agatgggcga ttcggaccgg
    20401 actcaggtgg gggaatgggt cgtcgcgatc gggaacccct ttggtcttga gcataccgtg
    20461 accgtcgggg tcatctctgc caaaaacaga agcattcggc tcgagagact caattttgac
    20521 ggttttttcc aaaccgatgc agccatcaac cccggtaaca gcggaggtcc gttgatcaac
    20581 ctggacaacc aggtcatcgg gatcaacacg gcaatcgtcc cttacgcaca gggaatcggc
    20641 tttgcgatcc cgatcaacgt cgccaagcag gttatgaacg atcttgtccg gtacggcagg
    20701 gtgaagaggg cctggcttgg ggtctacatg cagccgatca cgccggatat cgtcgaggct
    20761 tttggactga aatcctccga cggggccctt gtcagcgacg tggtcccgga ttctcccgcg
    20821 gaaaaggcgg gcgttcagcg gggggacatt atcaggaaag cagggagtgt gcccgtcaaa
    20881 tccccggacg atttgtccaa tgctgttcgg aaacaggcgg ctggagacag cctcaagctt
    20941 gaaatcgtga gggatgggaa acccctcagt ctgacggcga aactggaaga aatgccgagc
    21001 gagcgaagcc tcggcggcca gccacaagga aggatcggaa accgccttgg cctccagctg
    21061 ggtaacgtgg acctgaaaaa caggcagagg tttgatctgc cggccattca gggcgttgtg
    21121 gtccttggtg tcgaggacgg atcccctgcc caaaaagcgg ggcttcagcc cggggatgtc
    21181 atccaggggg tcaacggaag ggttgttcgt gacctcgttt cctgggaaag ggcaatgagc
    21241 caggtgagcg ggaaaaacct cgtccttctt gtctggagag aggggaggac gactttcgtg
    21301 cccctgaaag cccgctnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngggt
    21421 aggtccggat tcccaggcgt ccccccggag gggaatattt gctggcgttg tcgaggaggt
    21481 tgcaagcgag ggtgaggagg agcggttctc gcccgagcag cgaagcgggt ttgacctctt
    21541 tgaggacttc ctgtcccctt cggttgaata tcgggacgag gttctgacag gccacgtccg
    21601 cgatatgcat cagattcacc ctctcgagtt ccaggggctt ttctgcgaga tcaagccggg
    21661 aaagctcgag aagggattcg atcatgttcc tcatggagga aacatgatcg atgatttcag
    21721 cgagtttttt ctcgtaatac tcggtggatc tcgggcgcag cagggtaacc tctgccttgc
    21781 tgcgaatcac gctcaaggga ttgagcagtt catggctcac gttcgaggtg tagcgccgca
    21841 agttcccgat cgcctgcctg aaccgttccg agaaggaagc taaggcggcc tcaagcctgt
    21901 cacggtcctc ttttttcccg gggagtggcg gtaacggacc aggacgatcc aggttgagat
    21961 cctcaaaata ctccgccagg gcacgaagag tctcctgctc ggctgtctcc tctctggatt
    22021 gggtttctct ggtccgctcc tcatccatgg gtatttttcc ccaatcgcgc tccctcaggc
    22081 ctgatcgggg cagacgactt tgtatccgac tccgcggacg gtaaccacca tgaggtccag
    22141 gccaaagggg cgaagcttgt cgcgaaggta tttcacgtgg acgtccacca cgttgctggt
    22201 tccttcgtac tcttcctgcc agacgttcga gatgatcttg tctcgagtga agacctggtt
    22261 ttcgtgcctc atgaggagtt ccaggatatc gaattccctc cttctcaggg ggaccgtctc
    22321 ttccccgaca cggcactccc ttgccaatgg gtcgagcgtg agcggaccgc agcgaagcag
    22381 ggccggtttg ggctcggaaa ccctccggat cagggcattc accctggcga gcaattcccg
    22441 gaaatcgaac ggcttaatga gatagtcgtc ggccccctgt tcaagccccg tgacgcgatc
    22501 ctcgatcgaa tcccgggcgg tgagcatgat cacgggaacc tttttgccct cttccctcaa
    22561 cgctgaaatg atttcaaggc cgctgagctt tggcagcatc aggtcgagga tgatacaatc
    22621 atggtcaccc gcacgggctt ttttcaggcc atcctgaccg tcataggtga agtcgaccgt
    22681 aaagccgttt tccgaaaaac cttcggaaag gacttccacc agttccttgt tgtcttcgat
    22741 aattagcaat ttcatcggga tccccctctc aattctaatg aaatcgggat tttcattcta
    22801 ttaaaacaca ggaaagaaaa gttgtcgaga gggaaatctg cgttttttgc acaatctgtg
    22861 tcgtgatggt agaatacctt tcgacccggt tctcggatcg aggattcgga cctcgttctg
    22921 gggcttggga tttgtttgaa tcggggtgtc gaaatgatcg aacgtgagga aaaacagcgg
    22981 atcatcgagg agttcaaggt tcacggggct gataccggct ctccggaggt tcaggttgcg
    23041 atcctgacgc acaggattcg cagcctgacg gagcatctga aaattcacaa gaaagatttc
    23101 cattcccgca gaggcctcct catgatggtg gggcagagga ggaagctgtt gaactacctg
    23161 aaaacaaggg atttcaaccg ttaccagaca ctgatccagc ggctgggtct gcggcactaa
    23221 ggaacgcgga agtcgtgagg cggggacggg acgaccgtcc tcgccttttt ttatcatcgt
    23281 atggtatgct cttcaggacg aaaaaatata gaaagagtca aggaggctca aaagattatg
    23341 gttcaaacat tttcaatcga agtgggagga agggccctat ctttcgaaac cggcaggctc
    23401 gcaaaacagg ccagcggtgc ggtccaggtc acttatgggg agacggtcct gctcgtcacc
    23461 gcctgtatga cggagaagcc gcgggaaggg ttggattttt tcccgctcct cgtcgatttt
    23521 gaagaacgat tctactcggc gggaaagatc ccgggaggat ttatcaagcg ggaaggaagg
    23581 ccttccgaaa cggcaatcct tagcgcccgg attatcgacc gctccatccg ctctcttttc
    23641 ccggagcata tgaggaacga cgtgcacgtg gttgccactg tcctttccgt tgaccaggtc
    23701 aatcctccga atatccaggc gatcaatgcg gcctcgatgg ccctgactct ttccgaaatc
    23761 ccgtggaatg ggcctgttgc ggcgctccgt gtcggctgca tcgatggaaa attcgtcgtg
    23821 aaccccacgg aggaagaact ccctctgagc accctggacc tggttttggc cgggcacatg
    23881 ggcgggatca ccatggtgga ggctggtgcg aaagaagtcc cggaatcgtt gatggtggat
    23941 gccctggagt ttggccatga agagatcaag cggcttgtcc gtttccagct cgaagtcagg
    24001 gagaaagccg gaaaaccgaa ggtggaaatt ccagcccccg tcaggatcga agcgctcgac
    24061 gaggccatcc ggaagaatta ttacggggag atttacgaag cggtcggaat tcatgcgaaa
    24121 gggcaacgaa acgagaaaat cgccctgatc aagcagaagg ccgttgagac gcttgagggg
    24181 gagttccccg attcttcccg atacatcggt gaggttgtgg aagaactggt caagaaggca
    24241 atgcgttcca tgacggtcga tgaacgccgg cgtgccgacg gaaggaccat ggaccaggtt
    24301 cggcccattt cctgcgaagt gggagttctg ccgagaacgc atggatcggc ccttttcacg
    24361 cgcggtgaaa ctcaggccct ggtgacgacc accctaggaa tgatggggga ggatgagcag
    24421 gtcctcgacg gcctgaagat cgacgagccc tccaaacggt tcatgctcca ttacaatttc
    24481 ccgccttact ccgtcggcga ggtccggccg atgagggggc ccggaaggcg cgagatcggt
    24541 catggagccc ttgccgaaag agcggttcga catctgatgc cgtccgagga agagttcccc
    24601 tacatcgtcc gagtggtttc ggacatcctc gagtcgaacg gctcaagttc gatggcctcc
    24661 gtctgcggcg ccagcctggc ccttatggat gccggggtcc cgatcaaaag gccggtcgcc
    24721 ggcgtggcga tggggttgat caaggaaggc gaaaaggtag aaatcctcac ggacatccag
    24781 gggttggaag atcactacgg ggacatggat ttcaaggtta ccggcaccga ggtggggatt
    24841 accgccctgc aaatggacaa caaggcgggc ggaatcacgc gggaaatcct ggaaaaagcc
    24901 ctgggacaag cccgtgaagg gcgtttgcac attctcgggg aaatggccaa ggtgatccgg
    24961 gagcccaaga aggagctctc cccttacgcc ccccgaatgt ataccacaag tgttcccgtg
    25021 gattccatcc gggacgtcat tggtcccggg ggcaaggtga tccgtggtat ctgcgccgac
    25081 acgggcgtaa agatgaacgt cgaggatgac ggcaggattt tcatcgcggc gaccaacgag
    25141 gagtccgcga agaaagcctt gcagatcatt cgggatctga cccgggatgc agaaccgggg
    25201 gagatcttct acggaaaggt gactcggctg ctgagcttcg gggcctttgt cgaagtcctt
    25261 cccggcaagg aaggattgct tcacgtcagc gaaatcagca cgcatcatgt cccgaagctg
    25321 gaggacgccg taaagcctgg ggacatggtc cttgtgatgg tcaaggagat cgacgatatg
    25381 aaccgggtga acctgacacg gcgacgcctg ctggagaagg ctgctgagca ccgccttgcc
    25441 gagctgtacc ccgacgccta cgcgctggag cttgcccggg aagagcgcat tgctaaggcg
    25501 agcgctgcgg caggatcgga acggcctagg cgcggggaag gagaaacccg ggaccggcgt
    25561 cccggcgggg agaggcatga caggagagga agcgggccaa gagaccgcgg gaggggtccc
    25621 gttgagcgac attagagtga aagtgaaacg gggagaaaac gcgaagcaga ttccgctgcc
    25681 ccagtacgcg acttcaggct cttcgggggt ggatcttcgt tcggcggaag cagccctgat
    25741 cgaacctggg acctgggcga tcctcgggac cggtctccat ctggagattc cggaaggcta
    25801 cgaggcccag gtccggtccc ggagcggcct tgccttgagc cggggggtga tggtcctgaa
    25861 caccccgggg accatcgaca gcgattatcg aggggaagtg cgggtcatcc tgatgaacct
    25921 tggaaaagct cctttccagg ttgaacccgg tgatcgaatc gcccagatgg tcttttgccc
    25981 ggtcgccagg gcggaactgg aagaagtcga taccctctct tcgacggaaa gggaacaggg
    26041 cgggttcggc agcacgggct caaagtaaaa aagacgaaaa actgggagcg gccttcgggg
    26101 ccgctcccag tttttttgcc ctctgcttct gacttcggaa cgactagtgc cctttcttcg
    26161 tctcagcgtt catggcggcc ttcaaacacc agatgaagcc gccgaagacg caaaccagga
    26221 tgatcgtcat cgtcacaggc cacatttcca tgccctcctt ttcaagccgc ggaagctagt
    26281 acttcgcttc cagcttgtga gtggtctttt ctgccagcca gttgttcagg aagaagaaca
    26341 cgacggcagc gatgatccac tggtagtaca tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26461 nnnnnnnnnn ngccagttgt tcaggaagaa gaacacgacg gcagcgatga tccactggta
    26521 gtacatcgtc cccacggtgt aggtgtactt ggagatcggg agccacttgt accattcgcc
    26581 ggggtaccag gaaatggcct gccaggtcca ccagccgaag atgatgacga accacaccgg
    26641 gaagaggcag atcagccacc acatccagta atgcttgatg tccgagtcag gttcgatgta
    26701 ttcgacccag atcttgcgga tcccgatctt tatcgcaccg atggagaaga gaagccctga
    26761 aagcagcagt cccacgcccc aaacccagtc ctggttctcg aagaaccagt tgaacatgga
    26821 ggaaggcagc ccgcagacga ggcaggccgc gccgatcgcc cacgttgcct tcttagttcc
    26881 ccagccagcg tcctccagaa ggcggacgcc gagctggatc atcgaaattt ccgaagtcag
    26941 ggccgccatg atcagggccg caaagaaggc cagagccatg atgatgccgc ccgggatctg
    27001 ggtgaagagg ttcgtcaggt gaatgaacgc caggccgttg ttgccggact tgacggcagc
    27061 catcgggtcg ggggcgatgg cgaaaatggt cgggatgacg caaagccccg ccagagaagc
    27121 cgcagccgtg tcagcgaacg cggtcgttgc ggagttcagg aggatgtctt catttttctt
    27181 ggcgtaaacg aagtaggtga ggaagagccc ccagcctgcc ccggtggacc aggcgacctg
    27241 ggtgaaagct tccaggtaag ttcttccctt caggacatct tccatgcgga tcgtgaagag
    27301 gtattccagc cctttccatg ctccgggcag gaactgggcc ctgatggcga ggaagagtag
    27361 caggatgaaa atggagggga tcatgatctt gttggccttc tccaacccgt tctggatccc
    27421 cctggaaacg atgagtatgc tgacgagcag ggcgataatg aagaaaagag cgccctgcgt
    27481 gggattgttg atgaaagcat cccatagcgc cttcgagtcc agcccgggtt tgagcacccc
    27541 ggttgcggcg tacaggaaat agcggaagca ccagccgatg acgaccgcat agtaaaaggc
    27601 gatggccagg gaaatccagg cgacaagggc cccgacccag gtccatttct tgccgagcat
    27661 ctctttgaac gaaccgatga cgcccatccg ggagaccttc ccccagacgc cttccgctgc
    27721 aaggagcggg atggcccaga ggaaaagggc caggaaatac gcgatataga aagccccgcc
    27781 gccgttcgtg gcggcaaccc gcgggaaccg ccagatgttg cccgttccaa tggccattcc
    27841 cattgctgcg aaaacgagac cccaacggct tgaccactgc tcgttcgtgt tctcggtcat
    27901 acaacgtgcc ctcctttttc atcgggtggt agtcgggtgc aaaggcaaca gcgatcctgt
    27961 ttcaacctgc tccatggcat cccccctcct gaaatgtaaa cctggataaa atatacaaaa
    28021 acaaacagac ccccagttca acacagttga ttataaaggc aggatagaag aaatgccaag
    28081 gacatggcct gaacaaaaaa acttaaatta acagtcaaat gaaacaatgc ggatctggcc
    28141 gagttgtccg aaaacaagaa gcaggggtag aatggctcaa aagatcaaga cggaccgggg
    28201 ggattttcga tgccgcagtt tacggggaaa gaagggcaag tcctggagac agatcatcga
    28261 agcgcttggg agattttgaa ggaatggggg atccaccggg gcgctgtggc tgccttcgtg
    28321 aacgggaagc cgacggacct gaaggccgtg ctcgattttg atgccaccgt ggaaccgatc
    28381 ctggcggata gcgaaacggg actggaaatc ctgcgtcatt ccgcctctca cctcatggct
    28441 caggccgttc tgcacctgta tccagggacg aaagtcgcga tcgggccctc gatcaaggac
    28501 ggattttact acgatttcga gcttccggtt ccggtttcgg aagaagacct gcccaaaatc
    28561 gaggaagaga tgaagcggct tgcgcgggaa gccctcccgg tggagcggct tgagatcccc
    28621 cgggagaaac tgatggaaaa atacagggcc gcgggggaag tctacaaggc ggagcttctc
    28681 gcggatatcc cggatgaaac catttctgtc tatcgacagg gggatttcga ggacatgtgc
    28741 cgaggccccc acgtgccgga cacttccttc ctccggcaca cgaaacttct ctcggtcgcg
    28801 ggggcctatt ggcgcggcga tgagaagaat cgcatgctca cgagaattta cggcacggct
    28861 tttgccgaca gggaagcttt agaggctcac atcaagaaaa tggaggaggc ccgccggaga
    28921 gatcaccgga agctcggaaa agaactggat ttgttcagta tccagccgga ggggccagga
    28981 ttcccctgtt ttcaccccaa aggcatggtg attttgaata ccctgatcga tttttggagg
    29041 cgggagcatg tccggcgagg atactccgag atccggaccc cccnnnnnnn nnnnnnnnnn
    29101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29161 nnnnnnnnnn nnnnnnnnnn nnngattttt ggaggcggga gcatgtccgg cgaggatact
    29221 ccgagatccg gacccccctg atcctcgata aggacctttg ggtccgctcg ggccattggg
    29281 accattacaa ggagaacatg tacttcacgg aaatcgacga gaagccctac gcgatcaaac
    29341 cgatgaactg tccgggcggt cttctggtct acaagagctc gctgaaaagc taccgggatt
    29401 tgccgctgcg gatggcggaa ctcgggatcg tccaccgcca tgaacggtcc ggggtccttc
    29461 acggcctgat gagggtgcga tgcttcaccc aggatgacgc gcatctctac gttaccccgg
    29521 cccagatcaa gcaagagatt ctcgggatca tggacctgac ccgctacatc tatcgagatg
    29581 tgttcggatt caattatcac gtggagttgt cgacgcgccc ggaaaaggcc atggggagcc
    29641 tcgagatgtg ggaaagggca gaaaccgccc ttgaagaggc attgaaggaa agcggagtcc
    29701 cgtaccgcgt gaatcccgga gacggggcct tttacggtcc aaagatcgat ttccacctgg
    29761 aggactgcat cggaaggacc tggcagtgcg ggaccatcca gctggatttc cagatgcccg
    29821 agcgcttcga catcacctac gtcggggcag acgggggtca gcaccggccg gtcatgcttc
    29881 atcgaaccgt ctttggaagc cttgagcggt ttcttggaat tctgatcgag cattttgcgg
    29941 gagcctttcc ctactggatt gccccggttc aggtgaaggt catccccgtg aaacccgagc
    30001 tggaagaata cgcgaaagag atggaggcca gggtaaaagc ctggggtgtg cgcgtcgaga
    30061 tcgacctgag ggatgagaag ctcggaaaga aaatccggga tgcacaggtt caaaaggtcc
    30121 cctacatggt ggttctcggc gaaagggaga tggcggagaa aaccctgtcg gtccgggatc
    30181 gttcgctggg agacttggga acgatgactc ttgaggcttt caaggaaaaa ctggatttgg
    30241 agtttgaccc ccggcactga aaggcttgtg gaatctcgca aactgtggta tattccgccg
    30301 gtacgcttga gagaagaaga gggcccacct ctcacctgaa gacgccaaaa tggctgttgt
    30361 cgggtttcaa gccgattcaa gtgcgggttc aatcgcaagt ggatcaggtc ttagggggcc
    30421 gctcgaagag gcggtccctt ttatttttag ccggaggtga tgagctatag ctacaaaacc
    30481 ggatgaaccc aacgtgaacg aggagatcag ggctcaggag gtccttctca ttgatgatca
    30541 gggcgtcaag gtgggggtgg tcccaacgag agaagccctg aaactggctg aagagcgtct
    30601 gctggacctg gttgaagttg ctccgcaggc cactcccccg gtctgtcgaa tcatggacta
    30661 cgggaagtac aagtaccaga ttcagaaacg agacaaggac gctcggaaaa aacaaaaggt
    30721 tcagaccgtc aaagagatga agatgaggcc caagatcgac gagcacgatt acaactttaa
    30781 agttcgggca atccggggtt ttctggagag cggacacaga gtgaaggttt ccgtcttttt
    30841 ccgagggcgg gaaatggcgt ttctcgacag gggaaaggcg gttcttgaga aagtcgaaaa
    30901 ggacgtggcc gaatacggca aggtggagat ggaaccgcgc atggagggcc gttacatgag
    30961 aatgatggtt gcccccgtgc cctccacgaa aaagccggca tccaaagcgg agggctcgaa
    31021 gctggccgaa gaaggagaaa accgcgaata gctccccgaa cctgggggct gacaaagcgc
    31081 tgtttcgaat catacaagga gggacttgtt cagatgccca agatgaagac gcattcgggg
    31141 gccaagaagc gtttccgcac gaccggaacg gggaaggtga cgtaccagaa gggcggaaga
    31201 aggcatatcc tggaatcgaa aaattccaag aggacgcgta ccttgcgcag gaagggctac
    31261 ctttcagggg cgatcgagga tcgcgtgaaa gtccttctgg ttagccgcta agtctgaccc
    31321 gtcgttccag aaaccgaaag aggagtgatc tcccatggca agggtaaagg gcggaagcgc
    31381 cagtaacagc aagcggaaaa aactcttcag catcaccagg ggctactggg gaagaaaaaa
    31441 gaacgtctac cggcgtgcac gcgaggcgtt cctccgatcc atgacaaacg ctttccggga
    31501 tcggaaggca aaaaagcggg aataccggaa gctgtggatt atccgcgtca atgcggcggc
    31561 ccggatgaat ggcatgtcct acagcagctt catgcacgga ttgaaattgt cggggattga
    31621 gctgaacagg aagatccttg cggatatggc cgtcaacgac atgcccgctt tcgtcaagct
    31681 ggtcgaaaaa tccagggaag cgctggcccg ttagtcccca gctttcttcc tttcaggatg
    31741 cgaaggatca tagggttccc gcgcaaaacg gcgcaggaac cctatgatct ttcggaggcc
    31801 ttggtttttc tgcgggcaac gcttggtctt tttcgatata ctcttttaga attgccatgg
    31861 atgannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaacagc gaatccctgc
    31981 aggctttacg ggttcgatac ctcgggaaaa agggcgagtt gacccagatg cttcgctctc
    32041 taggcaattt accgccggaa aagcgacccg ccgcggggca ggcactgaac cagttgcgcc
    32101 aggaaatcga ggccgccctt gacgctaggg cccttctgat cgcgcgagag gaagatgagg
    32161 ccagggaggc cacggacttc gtcgatgtga cccttcctcc ccggggaagg ggatgggggg
    32221 gcttccatcc agttgtccag acgatgaatg aaatcatcga tattttcgtc gggatgggct
    32281 tttccgtggc ttacgggccg gaaatcgaag atgatttcca taacttcgaa gccctgaata
    32341 tccctcctca ccatccggca cgggacatgc aggacacgtt ctatctggac aacggacggc
    32401 tcctgagaac acacacgagc ccggtggagg tcagagcaat gcttcgatgg ggggccccgc
    32461 tgaggatcat cgtcccgggc aaggtttacc ggagagacag cgaccagacc cattccccga
    32521 tgtttcacca gcttgaaggc cttctgatcg acgaggacgt ttccctagcc gatctcaagg
    32581 gatgcctgaa cacgttcgtc ggggcgattt tcgggcggcc actggacagc cgtttcaggg
    32641 ccagttattt ccctttcacg gaaccctccc tggagatgga tgtcgaatgt tttgcctgtt
    32701 cgggacggaa cccctcctgc agaatctgta agggaacggg ctggcttgaa atccttggtt
    32761 gcgggatgac gcatcccaat gtcctccgct acggcggcat tgatcctgac cgatacaatg
    32821 gttttgcctg ggggatgggg ccggatcggg tcgccatgct gaagtacggc ttgaccgacc
    32881 tgagggtgct gttcgagggg catggatcgt tcctgacagg gggagggtac tgaatcatgc
    32941 ggatctcctg gaagtggctc aacgaattct gcaaaatccc gctttctccc gaagaggctg
    33001 ccgagaggct aaccgtgacg ggctgcgagg tggaaaacat cgaacgtccg tgttccgccc
    33061 tgaaagggtt ggtcgtcgga aagatcgaga gactccagat ccatcccgaa cgccccgatc
    33121 ttttcgtggc cgaggttgat ctcggcgggc cgcgtgccca gtgtgtgacg gctgcgcgaa
    33181 acctccggga gggggatacc gttccctacg gcgcgccagg aagtgtgatc gcggacggga
    33241 cggttctcgg aaagcggacg ttcgattccg tcgtcagcgt cggaatgatg ctttcggcag
    33301 aggagatcgg tcttcccggg atcgctgatg aattcggcat cctgagattg ccggctcatt
    33361 tgactgccgg agaggatcta ctcaaagccc tgaacctcga tgactgcatc ctggaaatct
    33421 cgattacccc aaaccgcggg gatctcttga gcctgatggg gcttgcccgg gaatgctacg
    33481 gagtgatcga gggttcaacc ctcgagaagc atgagcttaa gctgccccgg atcggagatc
    33541 cccgatccat ttcctgggaa ggagtgaccc tagaggatcc tggatgtccg ttgtatggcc
    33601 tttcgtttgc cccttctgtg cagatcgggc cctccccgat ggaaacccgg atccgactgg
    33661 ccttttccgg gatccggtca atcaacaata ttgtcgatgc gacgaactac atcatgttgg
    33721 taacgggaca gccgctacac gcattcgacc tggaccggct tccggcaagg gagatcacgg
    33781 tgcgcggagc gagggaaggg gagcgtttta gaacgctgga cgggcaggat cacacgcttg
    33841 cagaaggtga cctgttgatc accagcggag gcatcccagt ggccctcgcc ggggtcatgg
    33901 ggggagaaaa cagcgagatc aaggcggaaa cgaaatccgt cgccttcgag tcggccttct
    33961 tcgagccgct tcgagtcagt cagactgcgc gaaggctggg catggggacg gaagcgtcct
    34021 accggttctc ccgaggagta gatcccagcc gggtcttgcc gaccctgtcc tgtctctacg
    34081 agtatctttt gaaagatgca gcccagcatc atttcgagga aaacgtccgt gtcgtatcga
    34141 cgagatcctg ggagaagcgg aggatttcct tgaccaggag gaaaatgcac cgggttcttc
    34201 tttgggacga cctggagcag gcctcgaaaa tcctgcagaa gctcgatttg cgggaaatct
    34261 ccgcaacaga cgaaaaacgg gtctttgaga tcccgacctc ccgggctgat ctgtacatcg
    34321 aggaggactt gatcgaagaa gtcggccgct tgagggggta cgaatcggtc ccctcacgga
    34381 ttcctcccct tcttcatggg actggcagag tgggggagga agtggaggcc caccggagga
    34441 tccgcgtcgc tgccatgtcc aggggatacc tggaaatcgt gacctatagc tttatcaatc
    34501 ccaagtgggt ggattggctt cggatcccgg aaggggatcc cagatcccgc ggtttccggc
    34561 tgacaaaccc catcagccag gaacagtccc tgatgaggac gactctcttg cccgggcttt
    34621 tgcaggctct ccagacgaac ctgcgttcgg ggtggaagga tccgattcgg ctttttgagg
    34681 tcgggcgcgt cttcctgcct ggagaagaag gggaggacca tcgggagagc ttgagaatgg
    34741 ccggggtctc ctggaacgga aaggagcggc gggttcttca cgagacctcc ctgaaggatg
    34801 acgttctcag cataaaggcg gatgtgatgg cgctttgcgg gagccgcggg cagatccccg
    34861 gtttctctac gggacatgaa ccgttcggac atgccgggca aacggctcag gtgagcctgg
    34921 aggggaagcc atgcggatac ttgaccagca tcaagcctgg aatcttacgg gaactggaga
    34981 tcccgggtcc ggttttcgcc tttgagctgg agatcgaatc tcttttgccg gaaaaagggt
    35041 tccgcttcgg ccaggtggtt cgctatccgg ccgtgtatag ggatatttcc ctgattgcgc
    35101 cggtgaacaa gcccgccgcg tcggtcgagg aggatcttcg ccggcttgga ggaacgctgt
    35161 tgcgcgagct ggtccttttt gatgtgtatc aagggcccgg aattccnnnn nnnnnnnnnn
    35221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    35281 nnnnnnnnnn nnnnnnnnnn nnnnnngagc tccggaaaat cctccagatc atcgggaaac
    35341 actgccggag tgagctggga atcaaaatcc tgcacggggt tctcccggcg caagaccgaa
    35401 aacaactgga tcttcgccag gcgctccttc gaaatgtcct ccgttaccgg gagatcgagg
    35461 gggacttccc ctgggattca cggattgttc cggtaacagc cctccttgaa gaggcgaagt
    35521 ccagcgccct gatgtccggg gaagatctcg tccatgtccg gaacctgctt gcgctcgcca
    35581 cggaagtgaa aaatgaaatt cgccgcgtcc aggaacgttt caccgaattg gagcagatcg
    35641 ggaggagaat ccgggatttt tcagcggaac ttgccttgct cgacgtcctg gatgaggaag
    35701 gatcccttta cgatcacgct accccgcaac tgagagaaat ccggaaccgc atcgaatcct
    35761 tgaaagcgac gacgagaaaa acagggcagg ctcttctgaa cgatcccgca acctcgaacc
    35821 tgctccagga ccgggtcctg agcttccgaa acggtcgatt tgtggttctc gtcagggccg
    35881 aaaacatctc ctcctatccc gggatcgcca tcgaccgaag cggttcggga aattccttct
    35941 acgtggaacc gtaccgtctg gctgccctga acaaccggct ttcggccgca gtggaggagg
    36001 agcggcagga ggtttttcgt atccttcgaa ggatgaccct gaagatcctg gaacgggaaa
    36061 cggcgatcgc cgaggccgaa tcggcccttg gcagcctgga cctggtttgt gccgaagctg
    36121 aaatggttga aaagggtggc tggcatttac ccgaaatttc cctttctccg ggttttcgct
    36181 ttcacgggct tgttcacccg ctactgggcg aaaaagcggt gccaatcgac atcgcctgcg
    36241 gggatgactt tcgtgttctt gtggtgaccg ggcccaacac ggggggcaag acagtggccc
    36301 tgaagaccgt tggggttgct gtcattcttt cctggatggg gttgccgatc tcggcaagcg
    36361 agggatcggt cgtgggcgaa atcgattccg tgtacgtgga tgtgggcgac gagcaaagca
    36421 ttgaacagaa cctctcaacc tttagcgccc atgtgaaaaa catcgtttcg atcctgatgg
    36481 agacggggcc gggatccctt gtgctccttg acgagctggg atcgggcacg gatccccaag
    36541 agggggcagc cctgggaatt gccctgcttg aggccttcag ggagaaggga gcactcgttc
    36601 tggccaccac acaccataat tccatcaaaa cctacgctct cacgactccc ggggtggaaa
    36661 cggccagcat ggaattcgat gcggtttcct tgaaaccgac gtttgctctt cttatgggaa
    36721 tacccgggaa aagcaacgcg cttctcattg ccgagaggct gggaatgccc ccggcggtga
    36781 tcgagcgggc cagggccaac ctgcgaggcg aggatgtttc tgccgaagaa ttgatcggtc
    36841 agcttcagga aaaacgcgcc gcccttgaaa tcgaagcccg gaacctggcg gaagagagag
    36901 ctcggcttat cgcagccagg caatccctgg aaagccggat ttcgcggttc gaggcggcaa
    36961 aggaggatcg attgaaagaa gcggatcggg aagcgggaaa gattgtcagg gaagcccagg
    37021 aggctgcgag ggaactgctc aggagccttg agaggtcgaa aacggtccca gcggcacatc
    37081 gggcgatcaa cgccaagggc cgggatctgg aaaagatccg taaagaggct gaaatccggg
    37141 aagaaaaacg cattgaaaaa cgttccgtga aggcggaagg gatccgcctt cagccgggag
    37201 acaccgttca gatggtggat tcctccatca agggaatcgt cgaaagcgtt tccggggaaa
    37261 aggtcaccct cgttgccggc tccttccgca tggaggtccc catgaacagg ctgaaaaaag
    37321 cgggggaagc cctgtccaaa acaaagccgg agatttcagt gaagaccatc cgtcccgtcg
    37381 ggatcccggg atccatcatg attcggggga tgaccgtcga tgaagcgttg ccggaagtgg
    37441 aaaggtatct cgaccaggcc taccgggcgg gatacgatca ggttgccgtc attcatggga
    37501 ggggcacggg gacattgcgg aaggcagtca cggaacttct gaaaaccctt tcctacgtca
    37561 aggagtaccg gttgggcgaa catggagaag gcgggtacgg agtaacgatc gtgacctttt
    37621 caagatagac cgaggagggg cttttcatgg aagagggctt tttcaacctg aaatccttga
    37681 gagagcaagt ttacgaatat ctcaagatcc agctcaacga ggggaagctg gagcatgggg
    37741 ctttcctgaa cctgaacgcg atcagtgccg aaatggggat cagcaagact cccttgaggg
    37801 atgcgctctt ccagcttgaa gcggaaggat tcgtgacaat ctatcctcga aggggagtga
    37861 aggtgaacac ccttacgctg gaaaaaatcc gaaacatcta ccagatttta ggggggctcg
    37921 agggagccgt cctggtggaa gtcgcatcga agttccggaa aaaggatgtt gacgccatgc
    37981 gtcagttcaa tgaagaaatg aaagacgctc tccagcggaa cgactttgag cggttctact
    38041 cgaggaacct tgattttcac aacgcttacc tggagctgtc ggaaaatacc gatcttctga
    38101 ggaccgtccg gatcctgaag gaaaggcttt acgaattccc ccgaagcaag ggattcctga
    38161 aggaatggga agtcgaatcg accggagagc atgaccggat catcgaattc ctcgcaaagg
    38221 gggatttcaa cggggcggca gattatatcc gggacgttca ctggtctttc gcggtccagg
    38281 aaagatacat ccgcaaatat tatttcggct tggctgccca gactgccgag cgttaagctt
    38341 tgcccctgca ggcctggtgg aacagggcag cagcccggcc gatcggccca aggctgctgc
    38401 cgttttttta tttcccgacg tccaagcggg gcatggccct ggcggaaggg cagagagcca
    38461 acccaccccg ggcggtgcat cggagttcct ctggaagcat tcttccgaca ctgtctacgg
    38521 cgaccagggt ttcttccagc ggaaccgggt tttcgtaccc gcccagaacc aagtctgcac
    38581 agaggaaggc catggatgcg agagcggcat tcctgctgtg gcaggggatt tcaacaattc
    38641 cctgcaccag gtcgcaaacc gaccccatga tgttctggaa aaccgttgca gccgcatcgc
    38701 aggcctgccg ggcagtaccc ccggcggctt caacaaccgc agcggctccc atggctccag
    38761 cggccccgat ttcaacctga cagccgcaca cctccgccgc gaaggtgctg accctgtcca
    38821 gggcctgtcc taccgctccg gctgcccaca aggcatcgag aactttctcc tttgaaagtc
    38881 cgaaatccag gatcagagtg gaaaggatcc ccggtagcac tcctgcggat cccccggtgg
    38941 gcgccgcaca aacgacaccg ccggatgcgt taacgtgcat ggctgccatt gcccttgcgg
    39001 cggccctcag gtgcggtcca ccgaggaaaa ggcgcccttt tgactccgcg tccaagattg
    39061 aagatgccat cgggcgcaga agtttcatcc ctcccgtcat cgaaaggcca gaatcaacgg
    39121 cgttgagcat gacgaccagg cgctgctcca tttctctgga gagctcctcg cgggggattc
    39181 ccagcaaggc ttcttcgttg gccagtgcgg cctttcccag ggaccacccc cgttcttcgg
    39241 ccaatcgaag aagagcccac gtatcctgaa agagatcggt tcctttcaag ggagccatga
    39301 ccggcctgga ttgccggatg agggctttcg aggaaaactg gcgaaggcgg gaaagaagga
    39361 gctcatccgg cgattcgcag gcggaaacgt ggaaaaagcg ggaggnnnnn nnnnnnnnnn
    39421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    39481 nnnnnnnnnn nnnnnnnnnn nnnnnggaaa ggggtcagct cgaagcgggc ctgaagtccc
    39541 atctcggggg ccaactcgag catccggggg aaacgttcat ccgtaagagg gatgtcgagc
    39601 agtcctgaca ggagggccag gtcgctaccc tggtcatggt agcagacgga cagagaactt
    39661 gatgggtgaa tcaggaaacg agccattgtc ggttttgatc ccagcaggtc cctggaaatc
    39721 cggccgattc gccaggggcc cgcggtgtgg gaactggaag gtcccctcat aatggggcca
    39781 atcacgtgat tcaggatgct cactctcttc aatctaaatc cccctctttg ttggtgtggc
    39841 gtctcttcct ctccctttgg aatggacctt cttcttcccg tggttttccc cctccaatca
    39901 tagacctgtt gacagaggcg ggacctttcg cctaggataa ctctatccta catcaagata
    39961 tgtgatgtat tagatacctt tagttcatct ttttcgtcaa gccgattgaa tctccctcac
    40021 ctgattccga ggaacgatgt gaacccgtcg agaagaggag gtgaatcgat cccgccataa
    40081 ggatctgtga gcaccagcag agcagtccat gttgcaggca gcaaaaattc gcccacagaa
    40141 aggagtcacc gatccatgac caaaaccaga aagtccctgt tgctctccgc ccttcttttc
    40201 gtttccctct ttgccctaat ggcgagttcc gccctggctg ctccggccgt taccctgaag
    40261 ttcgccggac aatccccggc cgatcacccg gcctcgatcc tgatgaacga gatcgcaaag
    40321 gaagtagccc agaaaaccaa tggccgcatc gagatcaagg tctacccggc caaccagcta
    40381 ggtgactaca ccctcgtcta cgaggagctc atcaagggaa ccatcgacat gtcctgcacc
    40441 tccgtcccca gccagttcga tccgcggctg gaattggtct acatcaacgg tttcgtcaag
    40501 ggatacgggg atgtcaagag ggttttcgcc cccaacggct ggcttttcaa aaagatggat
    40561 gaactgaaca gccggctcgg ggtcaaactc ggcggcttct tcatcgaggg catgatcggg
    40621 actgccacga cgaagccggc agcctctccc ctcgatccaa aggtcaagaa gggcgtcctg
    40681 atccgggtcc ccaacatgga cgtttataag cttgccgccg aagccatggg ataccggacg
    40741 gttaccattc cctacgcgga tgtctaccag gccctgcaga ccggcgtggc cgaaggagtc
    40801 aacggattct ccgtcgtggc agcctacacc atgcttcgcg atgtcatcaa gtactggtac
    40861 atgaccaact attctctcga atgcctgaac tacatgatca gcgggaagac ctgggcaaag
    40921 ctctcccccg cagaccagaa gatcctccag gaagccatca accatgcggc tgcaaagagc
    40981 atcacgcttg caaaacagtc tgacgaaaag tacatgcagc tgatgcggga caaaggcatc
    41041 aaggtctata cgtacagcga gaaggaactg atcccgatcg ccaaggcctg cgccagcacc
    41101 tggccgatgc tcgagaagaa gatgaccaag gaactgatcg acgagttccg gaaggaactg
    41161 gcaccgaaat agacacattg gttctagaat ggacccaaag ggagaccctc tccctttggg
    41221 tccattcatc tcagtagaaa ggaaaaagcc cttcaaaggg aatctagttt tttagagggg
    41281 agatctgtcc cgtgcctcag tgtagcgatg cgatagagga aaaaccgggg atgatgaagc
    41341 cccggaacct attcgacaag attgtggtga atggtttttc agccgtcaca ctggtcatgt
    41401 ccatcctgct gacaatcctg attgcgctgg cgaccttcat tcgctatgtc atccagggcg
    41461 atctttacgg atacgaagag tgggttaagc tgtttgcctt ctggctgtat ttttcaggtg
    41521 ctgcgatcgg cgcattcaac cggacccacg tttcggcaga cctagtgcaa tcctatgtcc
    41581 ccgaaggcgt tctgaaacgg cttctcgttt tccttaagga cctcatcacc gtcgcggtaa
    41641 cattgctttt tgtctggtac ggctacgggt tcttcatgtt tggcttcaga ggtcctctag
    41701 gaacgggcat cgcgctgccc agaacaacga tttggcagat tccgctttgg acttcctatt
    41761 tggcgatttt cctggggttg atctttatgg ccttttactt cacccgggat cttttcctga
    41821 gtgcgaaggc cctgtttaag ggaggacgca aatgatttac gttgcgattg tcatccttct
    41881 tgccaccctt gttctcggag ttcccgtacc ggtcagtttc atggccagcg cggcctggct
    41941 ccttttcttc ggagggccgg acggggcggg ttacgaagcg agccaactcc ttccttatgc
    42001 tttcgcccag attaactcgg tatccctgat cgccatcgcc atgttcatca tggcgggggg
    42061 gatcatggaa cgtggaaaga tcggggaaaa gctcatcgat cttgtggacg ttttcgtggg
    42121 acacatccgg ggaggcctgg ggatcgtagg gaccatctcc tgcgcggttt tcggatcgat
    42181 ctcaggggct gcttgcgcga ccctctcctg catcgggacg atcatgttcc cgaggctgga
    42241 ggcggggggg tacccgaggg ggcactccgc tgcgctcatg gcgaacgcct ccctcttggg
    42301 gctgctgatc ccgccgaacg ctacactgat catcttcgcg tggctgagtg gccagccgat
    42361 cctggcgtgc tttttgtcca cggttatgcc ggggatcgtg acgattatcc tgatcagtgt
    42421 cgtgaacatg tggcttttgc gcaacaacaa ggaagtcctc gtggcccaca agcgaaccac
    42481 agcggaaaag tgggaattat tcaagacacg tgggaagctc gccatcccgg ccctcatgct
    42541 tccggtcatc gtcctcgggg ggatctacgg aggcatcatg accacaacgg aagcgtccgc
    42601 catggcggtt ctttacgcca ttccggtggg agtcctagtc tacaagggga tgaaatggcg
    42661 ggaagtcatc ggtccgctcg tggaatcggc cgtgacgacg ggtgtcatca tggccatgct
    42721 cttttctgtc tccatgctga gccgcctcta cctnnnnnnn nnnnnnnnnn nnnnnnnnnn
    42781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    42841 nnnnnnnnnn nnncgggtgt catcatggcc atgctctttt ctgtctccat gctgagccgc
    42901 ctctaccttt tggaggacct cccgtcaagg cttttggccc tttttcgagc cgtttcggaa
    42961 aaccgctggg taattctttt catgatcaac gtcttcctgg tgatcatggg gatgctcatg
    43021 gacgatatca gcgtcgtggt gctgactacc ccgatcatga tgccgattat catggagctg
    43081 ggctttaacc cgatccacta cgcggcgatc atcggggtta acactgccct ggggtgcatc
    43141 acgccgcctg cggcaccggt gttgtacctg tcaggtcggt tgagtaagac gcccataaac
    43201 gagatgatga cccccgccct gtacttcatg ctactctgct gggggcccgt gctgctcttc
    43261 accagctacg ttccgaaact ggttctgttc cttccccaca ttgtcctggg gaccccctgg
    43321 taggcaagga atgttttacc ggaacctgtt tgccgccctc tgttttcttt tcctggcctt
    43381 ggggattgtt ctccattcac cgcatgcctt ctgggtaagc ctgatgtgcg gggctttttc
    43441 ccgtacggcg gtgaagaaga agtggcccgc gcctcctttc gccgagaagc ttttcctcaa
    43501 gaggcagact gaagagagaa tctcggaatg acagaacccc cgtccgctaa ggacgggggt
    43561 tctgtcattc cgagtggatg tcaggttttt cccacttcgt ccccgtggct aacgatgacg
    43621 gatatgtccg aaacccttgt ttttccagga cccaggggct cccgagggca tgcggaaacg
    43681 gcgcatacaa gcggcagcag ggctgttagg gtcaggcgat ctcccggttt tgagacaggg
    43741 ggcaaacgga cgagggtccc gtcggtttcg aagagggtat tcccgaagac gttcagaggt
    43801 tctggaactt gattgcgtga aatttcccag ggggctaggg ctccctcgaa attgccgatg
    43861 cagctgtggt gatcatcggt cccccccacc caagagtcac acgcaggcat caggaaatcg
    43921 tgcgtgggcg ccgtgttctc ggtcacctcg agaagcggac gaagcaagtt ggatcgaagc
    43981 ctgtctttcc ccctgatgct gactgaattc agtgcaaggc gagtgtgtgc gggggagagg
    44041 aattcggaaa gtcgatccct gtagaaagcc acaaagacac aaacctgccc tccctcaacg
    44101 tcaatgagtg tgatccgatc ccccttttcg atttcaaaag ccctcgcgta tcctcccggc
    44161 accagaattt cctttctgta atagaggaat cccttcattt atcccgtacg ctccttcgga
    44221 atgtttcaac tgtcattgca ttataagtca ccaacgacgg cagagcgcga gattttcgcg
    44281 ttgacgatag ccggtaatcg tggtattttt tccgcgcacg gtgcgtgtcc gtagctcagc
    44341 tggatagagt gttggcctcc ggagccaaag gtcgcgggtt cgaatcccgc cgggcacgcc
    44401 ataaagcgat aagaagacca ggaaacgtca ggttcctggc cttttttcta tccaggaagg
    44461 gcataagcgg gcttggcggg gaggcgtgga catgggaaat agctggaaga aaaagttcca
    44521 ggagctcggt atggcgcttg aagagcaggg aaacgaagtt cttgaaagag tgagcgagga
    44581 agccttggcc atcgggaagc aggcgaaagc ttccctcagc cgggcaagga agaagacaga
    44641 ggagatcctg aattccattc aagagatcga agagatcaag gcccgtctcg atacgatcga
    44701 aaacgatcag caacggcttt cgaagctggt ctcggagctt gcgcaacaga tgaaagcaat
    44761 ccgtggggaa agtcggcaac gaacgcaggc agaagagggg gagaacgaat gacagtgccg
    44821 tcggacatcg aaatcgcgca gggagcgaag ctgctgccga tcggggagat cgcggagaag
    44881 gcgggga
//
LOCUS       DAOOVN010000006        43462 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_559_length_43462_cov_14.622365, whole genome shotgun sequence.
ACCESSION   DAOOVN010000006 DAOOVN010000000
VERSION     DAOOVN010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 43462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43462
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_559_length_43462_cov_14.622365"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..604)
                     /gene="rpsG"
                     /locus_tag="PLY83_01400"
     CDS             complement(134..604)
                     /gene="rpsG"
                     /locus_tag="PLY83_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOV28723.1"
                     /translation="MPRKGHVRKRTQAADPVFGIPALTKFVNCVMYCGKKSTAEKIVY
                     GALELSAKRLNTQPDEVFRKAMDNVKPVVEVKPRRVGGATYQVPVEVGPERAEALAIR
                     WIIQYARAKKGMPMVERLAREFADAYKGEGASMKKREDTHKMAEANRAFAHYRW"
     gene            complement(633..1004)
                     /gene="rpsL"
                     /locus_tag="PLY83_01405"
     CDS             complement(633..1004)
                     /gene="rpsL"
                     /locus_tag="PLY83_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOV28724.1"
                     /translation="MPTISQLIRLGREEKRERSSAPALQGNPSRRGVCTRVYTVTPKK
                     PNSALRKVARVRLTNGIEVTAYIPGVGHNLQEHSVVLVRGGRVKDLPGVRYHIIRGTL
                     DCGGVEGRRQSRSKYGARRPK"
     gene            complement(1022..1288)
                     /locus_tag="PLY83_01410"
     CDS             complement(1022..1288)
                     /locus_tag="PLY83_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HOV28725.1"
                     /translation="MPLDELASDRRVVGVRQVFRKIRSGQLQKLFLARDAEEDRVKEL
                     LEEAGKRNLEVEWCESMEILGRACAISRKAAAAGLLGENVKNIE"
     gene            complement(1405..6324)
                     /gene="rpoC"
                     /locus_tag="PLY83_01415"
     CDS             complement(1405..6324)
                     /gene="rpoC"
                     /locus_tag="PLY83_01415"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOV28726.1"
                     /translation="MSRREIVGVRIKLASTERIRELSSGEVKKPETINYRTLRPEKDG
                     LFCERIFGPTRSYECACGKYKRSGPKYKGILCDRCGVEVTDNRVRRERMGHIELAAPV
                     VHIWYLRGIPSRLSLLLGTATKDLEKVVYFAPIRRRKPVFKVVMEGRRTDLARRGDLI
                     DASEERIHSHYDPKFKAEEAYRVIQVDDVPLDEGDLVSAQQVARFKADFGDEIFKVEP
                     AFRIEQEGSEGPGEILSASELEARGGVEAFPGIQRAVVGNQEAFIVTAVAHLPFQKND
                     VLALSEVQLYNQKYPGRFQAQQETATIEDPCYLVIDGGESPFARGEIILEREQRLCAA
                     YDREFSAGIGAEGVQQLLNKLDLDEMAASLREEISETTGQKKRKLIKRLQVVEDFRKS
                     ASKPQWMILEVLPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLRKLQELRAP
                     EIIVRNEKRMLQESVDALIDNGRMGKAVLGAGNRTLKSLSDLLRGKKGRFRQNLLGKR
                     VDYSGRSVIVIGPKLRIYQCGLPKQMALELFKPFVMHKLVERGLAPNVKSARRLIERG
                     RDEIWAILEEIIKDHPVMLNRAPTLHRLGIQAFEPVLMEGKAIRLPPLVCTAFNADFD
                     GDQMAVHVPLSLEAQAEARILMLSANNLLSPASGRPVVTPTQDIVLGIFYLTAMRDGL
                     KGEGMAFRDIDDALTAIDHGVVHPNTRVKMLWKGEWIETTPGRALFNSVLHPDLRYIN
                     RQIGKKDLGSLLDKAYDKVGQAAIVDMLDAIKILGYQWSTRSGISFGLDCAVIPPSKK
                     TTVDETMKREEELSAQYGMGVLTEEEYLRQKEILWSEAARQIADDIVANMDQLNPLRM
                     MIDSGARGSKNQVAQMAGIRGLMADPSGRIIDYPIVANFRDGLNMLEYFISTHGARKG
                     LADTALRTAKSGYLTRRLVDVAQDLIITEEDCGTDKGTRISALIQDGKTVVTLADRIS
                     GRTALYDIEDPSSGDVLVRGGDEISQETALKIENAGIESVWVRSPMTCGLRNGICRKC
                     YGRDLATRKTVAIGEAVGVVAAQSIGEPGTQLTMRTFHTGGVRLTGEDITQGLPRIEQ
                     LFEVRRPKKVAQLVEADGRVLEIREMEGKRKVIIGKGEDEGEKIICTVPASQNLLVEE
                     GQLVKKGQCLTEGYIDPQQLLEVEGLEAVQKYLVDGIQEVYFSQGVSINNKHIEVILR
                     KVAPVNRVRITEEGDSSFVANELAWLDDLDQELDRIRKENERCLGEALDFLQGKTLRD
                     IEGQSGIDHVLSLKGKPLDADTIERLLQPGSAAREITVEDGEGLVRVILGEATFRREM
                     EGLELITPVSLENNTVIEPKSALELQDLLAITRGKPQILCVRDTETLASLERGAYLAE
                     DVVSGGKVFLSRDRALTPEALERLKEANASVVKIWRAPARHNIGDALNHYLIEKFFSR
                     PIKQAINPQGNPVNDVGRILDGRVVRGLVEGQIAAVELEGETQEILTREKLLGQVVSD
                     IAYGKVLLEPICNARGELLADAGTEISREVIDRLVQGEFQELVVRPISSSIETKRLIQ
                     RVSFVRRMREEPQWRPVVHGVTKAALATDSFMSAASFQQTAQILAQAAVRGDVDTLKG
                     LKENVIIGHLIPAGTGAEAYRHVEMQEKA"
     gene            complement(6325..9945)
                     /gene="rpoB"
                     /locus_tag="PLY83_01420"
     CDS             complement(6325..9945)
                     /gene="rpoB"
                     /locus_tag="PLY83_01420"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOV28727.1"
                     /translation="MTDFAPSKEHIQRRVFGRTKDLIGLPDLVEVQKNSYRWFFQAEK
                     EADSREPQGLQEILEEIFPIESYDGSFALEFVRYFVDESPITEEEARQRDLTWSMPVR
                     ATIRLVNRRNGEIKEEEIYLGDFPVMTERGTFIVNGTERVVVNQLARSAGVYFSSDTT
                     VPGGESYAAKVIPDRGAWLEFDLTPGDLLSVNIDNRKKIPVTMLLKAFGATTDEEILK
                     LFGAKPMQVDLVELHDEARGRLLAEAILDKEGQVLIRRNDRLTKEHLELLFEMGVTTV
                     WVWDVDNAIAATLERDNSRSGEDAVLELFRRLRPNEPARMENAKEYVYGLFFDTRRYN
                     LGRVGRYKLNKRLGLDIPESQRLLTVDDIVAIIKGILQLRNGEEKEDDIDHLGNRRVR
                     AVGELLQNQVRIGLLRMERIAKERMTTLPDLSSATAKDLINVRPISASLREFFGSAQL
                     SQFMDQTNPLAELTHRRRLSALGPGGLSRERTGFEARDVHYTHYGRVCPIETPEGPNI
                     GLVTSLATYARINDYGFLVTPKRRVVEGRVTDEIVYLSADEEDEFFVGRANTPMDEQG
                     RILGPDVHVRFRGEIETVEPEKINFLDVSPKQIVSISTALIPFLEHDDANRALMGSNM
                     QRQAVPLVLPQAPIIGSGIEHHIAKDSGCCVRARRSGTVEFVDASRIEIRTESGSLDS
                     YNLTKFRRSNQGTVIHHRPIVSNGDRVEAGEIIADGQSVDHGELALGRNVLVAFVSWE
                     GYNFEDAILLNERLVQEDHYSSIHIEEYEVEARDTKLGPEEITRDIPNVGEDALKNLD
                     ENGIVRVGAEVRAGDILVGKVTPKGESDQSPEEKLLRAIFGEKAREVRDTSMRVPNGE
                     GGKVVSVKRLTIDQNSDDLSPGVNEVVKVYVAQLRKISEGDKIAGRHGNKGVVSKILP
                     VEDMPYLPDGTPVDVVLSPLGVPSRMNLGQVMETVMGFVAVHNGWKVSTPVFEGATEQ
                     DIFDGVRKLAEGPYPEMAEDGRITLYDGRTGEAMENKVTVGYMYMLKLIHLVDDKIHA
                     RSIGPYSLITQQPLGGKAQFGGQRFGEMEVWALEGYGAAHILQEILTVKSDDIRGRLK
                     TYERIVKGQNLTKPGVPESFRVLVKELQGLALDVEIMYDDGTVGEILLEDEDEEMGAP
                     GTPRPRPAIVDLESGEDLLPPSAAGVTEEMLFVSPKELDSSFEEEEEPDEQDLTREGE
                     DA"
     gene            complement(10083..10169)
                     /locus_tag="PLY83_01425"
     tRNA            complement(10083..10169)
                     /locus_tag="PLY83_01425"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10133..10135),aa:Leu,seq:cag)
     gene            complement(10229..11224)
                     /locus_tag="PLY83_01430"
     CDS             complement(10229..11224)
                     /locus_tag="PLY83_01430"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOV28728.1"
                     /translation="MRVMVSEALSARDMEILREAARILREGALVVFPTETVYGLGANA
                     LDKRAVRKIFEAKGRPSDNPLIVHVSEPAGAEKVAFVPELGIDLMKRFWPGPLTLVMP
                     ARSVVPEEVTAGLKTVAVRMPAQRIALELIRLADLPVAAPSANLSGRPSPTDAAAVEE
                     DLGEKVDIILDGGSTAFGVESTVLDITGERAVLLRPGGTSLESLADFFGYCPLQAGAG
                     APVSRRSPGTRYRHYAPKVPLFLREDESVWAKVAAWEPGERAYIGIRPPVVPFGCVRL
                     FPDVETYARCLFSVFREMEKAGAKVIVADWPEEAHVGLALRDRLRRASGGSFNPQ"
     gene            complement(11221..12342)
                     /gene="tgt"
                     /locus_tag="PLY83_01435"
     CDS             complement(11221..12342)
                     /gene="tgt"
                     /locus_tag="PLY83_01435"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOV28729.1"
                     /translation="MFEFRLEARCPVTGARAGVLRTPHGPIETPVFMPVGTQATVKAM
                     TPDELSGMGARIILSNTYHLFLRPGSKLIREAGGLHRFMDWKGAILTDSGGFQVFSLS
                     DLRKVTDEGVTFRSHLDGSSHFMTPERAMEIQEDLGSDIAMCFDECVPYPASESASLE
                     AVLRTTRWAQRCRAVHRKEDQALFGIVQGSVFEEHRRRSAEALMEIGFDGYAIGGLSV
                     GEPHSEMYRILDVLNPVLPEDRPRYLMGVGLPSNLLEGVSRGVDMFDCVLPTRTGRNG
                     TVFFSGGRLNLKNATFARDFSPIDPECDCMVCQRFTRAYIRHLYKAGEILAARLCSWH
                     NLHFLIRLMKKARESIVKGTFPEFRQAFLERFGDGGPAS"
     gene            complement(12335..13126)
                     /locus_tag="PLY83_01440"
     CDS             complement(12335..13126)
                     /locus_tag="PLY83_01440"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOV28730.1"
                     /translation="MRLLDSHCHLNMEEFAEDLDLILRLAREARVERTLVAGFDEPSS
                     GKALALAKAHSAEGVYAAVGVHPHDAKTCPPAGLPESLQAWAADPRVVAIGETGLDLH
                     YNLSPRDVQEESFRRHIALAERVRKPLVVHVRDAYDEAIGILRDEAARGCTGVIHCFA
                     GEWRHAAEALEMGFFLSFAGPLTFPRAAALREVAAKVPEDRLLCETDSPYLAPQPYRG
                     RRNQPAYVAAVYETLAEVRKVSLEVLAEQVWRNAQRLFGWGDGNV"
     gene            complement(13214..15142)
                     /gene="metG"
                     /locus_tag="PLY83_01445"
     CDS             complement(13214..15142)
                     /gene="metG"
                     /locus_tag="PLY83_01445"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOV28731.1"
                     /translation="MNREPFYITTPIYYVNDVPHIGHAYTTIAADVSARFHRMMGQPV
                     LFATGTDEHGQKIQQAAESRGLTAQQLADQTVENFKKLWEALGISNDDFIRTTEERHF
                     KVVTAVFEKLLAQGDVYKGTYEGWYCVPCETYVPEAQMGEGKTCPDCKRPLEKMTEES
                     YFFRMSKYQEPLLRFYEENPDAILPRTRYNEIVSFIRSGLKDQSISRTTLKWGIPVPG
                     DDRHVIYVWFDALINYLTVCGYPDEEAKWKQYWPHVRHLVGKDIIRFHCVVWPAMLLA
                     LGVNPPRQVFSHGWWTVEGEKMSKSKGNVVDPFEMVDLYGKDAFRYFLLREVPFGLDG
                     DFSEIALVKRINSDLANDLGNLLNRTLQMIERFKGCTVTACGPVDPLDLEMARLATST
                     FETVRARMSEFAFDEALKAIWSFISRMNKYIDETAPWKLGKENDPRLDSVLGTLYQAL
                     IQIALMVAPFMPETACRMWRQLGLAGDPASALFEDYPWGFLPEGTTIRRDEVLFPRID
                     LKAWEEAKAEREAKKNLLPDPGEHEAEVGVEEFRKIELRTARVLSVEPVPNASKLYKL
                     NLDLGYERRTIVSGIREFYEPAELVGRDIVVICNLKPAKLRGVVSNGMLLAAESPDGK
                     ELALLTLDRPITPGSRIH"
     gene            complement(15139..16026)
                     /gene="rsmI"
                     /locus_tag="PLY83_01450"
     CDS             complement(15139..16026)
                     /gene="rsmI"
                     /locus_tag="PLY83_01450"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOV28732.1"
                     /translation="MPLIVVPTPVGNLEDITLRAIRVLREADWIACEDTRRTLRLLNH
                     LGIRAPLVSSHEHNEREKAPFLLKALREGKRVALVTDAGTPGISDPGAFLVNEAIREG
                     IEVEVLPGATAFVPALVLSGLPIQTFQFAGFLPPRKGDRQRRLLALKDVPCTLVFYVS
                     PHRLSDEVDDCARVLGNRPCALVRELSKVHEEVLRMNLDDLRESVRTPMKGEMVLVVA
                     GASQDPEKTEDCPSESWEKLVDAAVEEGLSGKDVVKMVYERYGIPKNRIKAYLIENAT
                     KQGESPLSGQGSAKRRIKG"
     gene            complement(16017..16763)
                     /locus_tag="PLY83_01455"
     CDS             complement(16017..16763)
                     /locus_tag="PLY83_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOV28733.1"
                     /translation="MRAGTSRDDILFGELNLIQPLEGPRVSVDTILLAAASRVKRGDR
                     VLELGCAHGAVLFILARKYPEAAAFEGLEIAEDLVEIARENARLNGLDGRVHVSRGDL
                     REIRKLYAAESFDVVLMNPPYNDPGRSRVSPRSAKAVARHGTTCSLADVFSAARFLLK
                     PRGRFFLVLRAARAAEALTLLVQKGLEPKRIRFVHPGPGKEASTFLLVAMKQGRGGIV
                     VDPPLFITDESGNYTRELLSAYRVESSPCL"
     gene            complement(16760..20182)
                     /gene="smc"
                     /locus_tag="PLY83_01460"
     CDS             complement(16760..20182)
                     /gene="smc"
                     /locus_tag="PLY83_01460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOV28734.1"
                     /translation="MYIAQLLLKGFKSFGGEHELQLSPGLTCIVGPNGSGKSNLLDAI
                     RWVLGEGSPSELRISRQTDLLFQGSASLSPCKEAEASVRLVSGKKDCRIRRKIDREGN
                     GLLTIDGNRARLQDLDQLKRDWGLEGIRFAFIGQGEISEIIQQKPLQRRMLLEGLFGI
                     DLYRRKREEAVDRLRKAEDELLRLLTLLSELSARRDEIAPMVEKARKAKEVMDLLEDQ
                     RRLYYWLRRERSEIALEALKTRREVLIREQARHSFWTAGWMRRKGLLQKELESLQKEM
                     EDCRRRQAAVSSSLQETLRQGAALASSARMNKDQVKTAQADCLAKKETLRVLEREASV
                     QRDLLQSLEAELSRKKAEAIALREDRLLRRQRWEKSEQELRSLTRRRAELQAEEETQR
                     ARLAALGRQWASLEAERRGLQEEFSRLETVGAEREAQVLSLVRSAEQAVERHAVAFAA
                     SQRTATELARLRKRENQVTQELEGLNQKLDGQVFPKPVQFLIAASRLGRLAVSPVPVI
                     EAFECPGRFEKAIESYLGARQFWLLVRDMEEAGLCIEKLRQSGAGRATFLPLDRCRGG
                     KPARDPGLTREPGVLGWALDLIVAEKEWKKAIEHLLGDLLVLENYRFASDLVRRLPRL
                     AVATLDGEVFMPGGSISGGAQKTGTGGMEIRRRTKETEALLENLQGEIRRLETELARA
                     EAMEIEASEEKDQAFSARREAEEALRLLHARKDRTEKDIRQLADTERKLKATLSEAGK
                     SLLALVLERRQAEAKIGSLEKEKEKENFLPDEGPTGLETEVLLLEEKQRGTLELLKRV
                     EKEVRGGRESLKTEEARIASRESALEKLFEDLSRLGQNYLALWRTKSGLEEMERTIAG
                     RLEKDQKFLVRVVAREGRAGEASRRCLTRLEELEREILEAENGLAELVTEWEEKFPYP
                     GPGRCGEIDLDEVKRSIRNAEKVLRSMGEVELGVLSEEESLLERTAFLSEQSSDVREG
                     IRSLQGFIAETDREASRVFGNAMDGIDRRFNSLFQRLFGGGEAHLQVEGPQEGWEAGV
                     DFTARPPGKRPQGLAQLSGGEQSLTAIALLFAAMEEARVPIAILDEADAALDEVNLRR
                     YVELAAEYARELQILAMTHRRTTMEKADVLYGVTLSEPGLSQLVGVRMEDWT"
     gene            complement(20148..21623)
                     /locus_tag="PLY83_01465"
     CDS             complement(20148..21623)
                     /locus_tag="PLY83_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOV28735.1"
                     /translation="MSSERQIIANVLDSEEMRLAILEDGRVAEIFCERMWETQKAGEI
                     FKARVDSVLPGMNAAFVNLGDGRNAFLYLEDARGVDIRANTEVVVQVVKTARKGKGAR
                     VSTRLSLPGRFLVLVPGGNETGVSKRILEEGERKRLRQIARALRPEGFGVIVRTVAEG
                     VPGEALQADLSMLLEQWAEIAALAERQPAPSLLYRDIGLVGRILRDELNGDVSEIVLD
                     CPEEFEKVSAYVRDFFAVNPPRVELYGGSVPIFEFYGIEKELAAALERKVWLPSGGTL
                     VVDQTEALTVVDVNTGKYVGATSLRETVLKTNLEAAEAVYRVLRLRSIGGIVVVDFID
                     MEYEEDRNILLQRLEEVFRHDRCKARVYGVTQLGLVEITRKRARSDLRGTFTKSCPFC
                     SGNGWVLKEDTVALSIKRFLRKVCRTGKAEALILELYPAVARHIVENYLESWEEEFER
                     KIFLREMPNFPWEKFRLDFQGSQSQAEHKFNVYRSTAAQGI"
     gene            complement(21641..22240)
                     /locus_tag="PLY83_01470"
     CDS             complement(21641..22240)
                     /locus_tag="PLY83_01470"
                     /inference="COORDINATES: protein motif:HMM:NF021610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HOV28736.1"
                     /translation="MSRVRFLYAKRGLSAFIPHVELPPLLARSARRAGLSLLLTEGFS
                     PHPKISLGPALPVGVPALAEPAEIWVGNWEQRALEDWQRGMPPGFSLLCASIVEGPTL
                     SRLCKAGEYRVYFRNPFPGEGPLRLAVESALQEPEILCAMDFDGDSARIVLAQPDRFG
                     PGFLVKAFRASGLVEGWKDLLILRTTVGGWEMNTVKSLV"
     gene            complement(22237..24060)
                     /locus_tag="PLY83_01475"
     CDS             complement(22237..24060)
                     /locus_tag="PLY83_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HOV28737.1"
                     /translation="MNSPLWSLLTTVKQPARYIGQEWGSNPGKSGGATPGLRVCLAFP
                     DVYEVGMSFLGFQILYALGNAQEDTILERAYCPWIDMEESLRASGLPLSSVESQTPLN
                     RFDAVGFTLQYELTATNILTMLDLGGIPLRSEDRGENDPLVLAGGPGALAPEPLAPFF
                     DFFCLGDGEELFPKVLRVISETRGLSREQRLSCLGEIPGVYVPQLASCEFSGTGVKIR
                     SKQPLPIRRQFVRDMDSVFVPSSLIVPNTGIIHDRIPVEVFRGCTRGCRFCQAGMIYR
                     PLRERSPERVVEIAMGLLEKTGWEEIGLVSLSSCDYTGLSPVLEALSGQLAEKGVKLS
                     LPSLRMDAFSVELAAQLGTVRKGGLTFAPEAGSQRLRDVINKGIDEGNIRMTLESMSE
                     HGWDRVKLYFMVGLPTETESDLQGIVEISKEAFRILRKLHRRAEVSVSVNGFIPKPHT
                     PFQWEAQLPYPETEARCKWLKRQLENPRISLNYHDPRQGFLEAVLARGDRRLAEVIER
                     AWRNGCRFDGWTETFHFDTWMEAFDSEGIDPAFFANRQREADEDFPWDHVDTGVSRSF
                     LWRERENAFRERITPDCRTGNCQGCGFSPTDCPRLKEEKGP"
     gene            complement(24093..25220)
                     /gene="rodA"
                     /locus_tag="PLY83_01480"
     CDS             complement(24093..25220)
                     /gene="rodA"
                     /locus_tag="PLY83_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOV28738.1"
                     /translation="MTFKEEKHDILRLVFVLSDRKLLFAAAGLFLVGLFSIFSASLGM
                     EKHAGVFVIRQAIWGAVAFAAYTAVLKIGYARLLEMAYRLYGVALLLLFSVLILGHVS
                     RGAQSWIGLAGLRLQPSELTKVALALLLAKHLCRHPVEGLKDLAGVLALSGISLILVL
                     AQPDLGSAIVLSVMVFTALYAAGTPVRYLWGLAGLALFAFPVAWHFLKEYQKLRLMVF
                     LNPYIDPLGAGYNVIQSRIAVGSGRLLGKGFMMGTQSKLGFLPEPHTDFIFSVFAEEF
                     GFLGGVALLGLFCFLLWRIIAAGVKARDLRGKILVASIAGWIWFQAVEGMAMSMGIAP
                     VTGLPLPFLSYGGSSLLALGMALGLAQSVFLENPKPDDDLF"
     gene            complement(25217..25504)
                     /gene="minE"
                     /locus_tag="PLY83_01485"
     CDS             complement(25217..25504)
                     /gene="minE"
                     /locus_tag="PLY83_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HOV28739.1"
                     /translation="MNFLARLFQVIGKEKSKETAKKRLQLVLIHDRTDISPEMMENLR
                     KDLIGVISNYLDIEEEKIELDFSKEERSVALVASIPVKSVKRRAERRAEPA"
     gene            complement(25517..26317)
                     /gene="minD"
                     /locus_tag="PLY83_01490"
     CDS             complement(25517..26317)
                     /gene="minD"
                     /locus_tag="PLY83_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HOV28740.1"
                     /translation="MDPRIIVVTSGKGGVGKTTTTANLAVALAKAGYKTAAVDGDIGL
                     RNLDVVLGLENRIVFNLVDVVEGACKLQKAMVRDKRVENLFLLPAAQTRTKDAVSADQ
                     MLKLCDELRENFDITLIDSPAGIEQGFRNAAIGADEAIVVTTPEVSAVRDADRIIGML
                     ESMSKSPIRLVLNRLRPAMVKSGDMIDVKDVLDILAIELIGVIPDDTSVVISTNKGEP
                     LTLGDESLAAKAFSNVARRILGEDVPLLDLDAPDQKGFFGFLRKALGL"
     gene            complement(26349..27023)
                     /locus_tag="PLY83_01495"
     CDS             complement(26349..27023)
                     /locus_tag="PLY83_01495"
                     /inference="COORDINATES: protein motif:HMM:NF015716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HOV28741.1"
                     /translation="MEKEKEKPAVVLKGGAGHLRLIVPEGLPDPEVWPALEKAIQEAH
                     HLLEGSRVVLDLQGRPLTEEIIGRMLKDVIWNYGLSVLSWVSFDIGGQSLLRKAGLSV
                     GEPAAALSEPRQVRSVQTLLLDRSLRSGQKVEHPGDVFIIGQVNDGAEVVSAGNIVVW
                     GRLQGLVHAGAEGNVSSRVIAKSFEATQVRIGRLFSTFDSASPWYGKSVLFAVEGDAL
                     IAKELP"
     gene            complement(27038..28777)
                     /gene="mrdA"
                     /locus_tag="PLY83_01500"
     CDS             complement(27038..28777)
                     /gene="mrdA"
                     /locus_tag="PLY83_01500"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOV28742.1"
                     /translation="MNRNIGRRTQLNLRLNHWRVFMVYSFLALAVSLFFFQVLRGGSY
                     AALSTQNKIRVIRTSSPRGEITDAHGAPLAVSVRTFDIVGYPPDLRKAGALEEVAALL
                     QRQGIPATAQGLEETIQKQYWAPYRPVTVASNLTLGQVATLVSDPFFPKFLFPTPVFR
                     RVYPAGPLAAHVVGYVGEVTREEMDSRAEENYRAGDLIGKTGIELVHEATLRGIAAEE
                     AVEVDALGRRRKRLSMTPPVKGKDLRLTLDLGAQREAQQLLGERKGVILAMDVRDGAI
                     KVLYSAPSYDPNPLTWGVSSSEWAKLLKDPDRPMMNRAISGAYPPGSTFKPVPALAAL
                     VEKVVTPGTTVYCPGQFKLGNRVFRCWKKGGHGTVNLVTALKDSCDVYFYQVGLWLGA
                     DRLLDWSRKMGVGQLTGIDLPGESRGNLAGREWKKRRFSESWFQGDTVNYSIGQGFLL
                     MTPLQLARVYAAFANGGTLVIPHLSAEEHPAGIDLHLPPEALGWVRKGMEEVVRAGTG
                     RASGVFGVSLSGKTGTAQNPHGEDHAWFVGYAPSDRPRYVAVALVEGGGHGSSAAAPL
                     VGQILAYLVGVER"
     gene            complement(28770..29231)
                     /locus_tag="PLY83_01505"
     CDS             complement(28770..29231)
                     /locus_tag="PLY83_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV28743.1"
                     /translation="MGILWISLLARDLLQVAFSGTALVPDLTLFSLIWLSVVRCEEPE
                     PLFLHAFGAGLLWDLRWTGVPGMTSASYALTVAVTAWVWSSLPPGRRANPVLFFFWSS
                     AAHVLTSVFPAIFTSKGLGLSSSLATQQGMAIACLLGLTALYIRHCRSHHE"
     gene            complement(29231..29995)
                     /gene="mreC"
                     /locus_tag="PLY83_01510"
     CDS             complement(29231..29995)
                     /gene="mreC"
                     /locus_tag="PLY83_01510"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOV28744.1"
                     /translation="MGRTRTKRWIVFLGTLSLSLFLAAVSPGSLPAEKAMDLVERVMA
                     VPEWPSRMVRDLVVKAVDLVAGERSLRERIDRLEQENLALRGLLQTTVNYGLGTGAGE
                     GHVTLRPPTSWWSEIRIDKGSREGIRPGMAVVQDGALVGKISRVESNFSWAELVTSSS
                     LYIPVVVDETRDLGVLNGDGNGLVLLLYIPVEKDLKPGMSVSTALVSELLPPGIPIGR
                     IAGAGEEIGGYRLYRITLKADLSRLYKVKVMTGGRS"
     gene            complement(30012..31067)
                     /locus_tag="PLY83_01515"
     CDS             complement(30012..31067)
                     /locus_tag="PLY83_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOV28745.1"
                     /translation="MFRLFSGFFGSDVGIDLGTVNVVVYVKGKGIVINEPSVIAVRRT
                     QRGGRKEILAVGREAKAMVGKTPSGIETVKPLKEGVISDFDMTEAMISHFVRAADERR
                     GFFSRPRVVVSVPACVTEVERRAVVDATLSAGAREAYVVEEPLAAALGASLPIHEPRG
                     NMVVDIGGGTCEVAVLSMGGIVVCKSIRGAGDEIDSAIVSMLRLNHKLAIGEGTAEQI
                     KWALGSVLPLEEELQMEVKGRALTDGLPSSILLSSIQVREAIEPIIQRISETIKETLE
                     STPPELVRDIYEQGLVLAGGGALLRGLSQRLSKELNVPVFVAEEPLLAVTKGLARILE
                     DLDSKRPILNTVERGSF"
     gene            complement(31090..31749)
                     /gene="radC"
                     /locus_tag="PLY83_01520"
     CDS             complement(31090..31749)
                     /gene="radC"
                     /locus_tag="PLY83_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOV28746.1"
                     /translation="MSLASLPAKERPRERLLQLGPEALSLQELLAVLLRTGQKGSDVM
                     SLAARILREYPDERALARATPAELCSFPGLGAAKAAALVAAVELGRRFCCNGQNGGRG
                     RWQDSLRKLEQTMVSEEREWIVSLFTDREGRVLGSEKLSFGGLDGAFLDAPYLCRRAV
                     RIGAAGLVLIHNHPDGNPRPSEEDRSLTIAVSKQLKLLGIELVGHFVVAGGSSRPVTV
                     H"
     gene            complement(31746..32372)
                     /locus_tag="PLY83_01525"
     CDS             complement(31746..32372)
                     /locus_tag="PLY83_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOV28747.1"
                     /translation="MEYRRFPMGALWTNAYLIWDREGTAILVDPGGDPSEALDFAERN
                     GLRIEWVLLTHGHGDHIAGLDAARGKSARGVAIHRLDASMLAAAEENLSRWLGAPIQC
                     SEAEKSLEDGEILPAGKMTVRVIHTPGHTAGSSCFLVSEGEETVLVSGDTLFARSVGR
                     TDLPGGSEKDLKASLRKLNDLADALPVLPGHGPETTLGRERAENPYWP"
     gene            complement(32375..32824)
                     /gene="dtd"
                     /locus_tag="PLY83_01530"
     CDS             complement(32375..32824)
                     /gene="dtd"
                     /locus_tag="PLY83_01530"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOV28748.1"
                     /translation="MRSVVQRVSWSEVKVDGQVKGKIGSGLCVLLGVTHGDGEKDVAW
                     MAEKLVNLRIFEDDQGKMNRSVRDVGGEILLVSQFTLYGDARKGRRPSFTLAAPPDEA
                     ERLYEALGAALREQGVPVATGVFQAMMQVSLCNDGPVTLILDSRGVF"
     gene            complement(32831..35041)
                     /locus_tag="PLY83_01535"
     CDS             complement(32831..35041)
                     /locus_tag="PLY83_01535"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOV28749.1"
                     /translation="MRDSFLGRIEESQRVASVKLAWQELWGKASRYLSKDELTRLGEA
                     MVLASKAHEQQKRSSGEPYIVHTISVASILADMELDVDTLVAALLHDVLEDTDLSGDT
                     IRSAFGENVLLMVDGVTKLGKLPFNSFEDYQAQNLRKMFLVMAKDIRVVLIKLADRLH
                     NMRTIQTLRRDKQVRIAKETVEIYAPLAHRLGIYQIKRDLEDLSFKVLDPEMFYEIRR
                     RVRKKLPEREQIIKQAIDILNQKMKEIGIEVSITGRAKHFFSIYEKMQRKNLSLEQLY
                     DLLALRVIVGTVAECYSVLGIVHTIWKPIPGQFDDYIANPKSNMYQSLHTTVIGPSGE
                     PLEIQIRTREMHWLAEYGIAAHWHYKEPGTRLNEMDKRLAWVRQALEGQGETPTEFME
                     NLKGDVLTSEVFAFTPNGDVVSLPAGSTPLDFAYMIHTEVGNKYVGAMVNGKIVPIDY
                     EIQNGEIVRILTSPQGKPSRDWLKIARSSKARSKIRAYFRQIERTEREERVIKGRELL
                     ERELRRREPGLEWNAQAMAGALNRVAHDLGYSSGEDLIAAVGGNSQSASTVAMKLLET
                     RVAPQQEVSLETSQAAATSAMARKEFDTEIVVEGAGGVLVSLANCCCPVPGDAIVGYV
                     TKTRGITIHRSDCRNIEEAKSERAVRVEWGRLISDRYTARLKLEGMDRSGLFADVSQA
                     ISSMDGNIIGVKASVVAANRARMTIEVRVRDVEHLYRIMAKLNTVNGIIGVFRG"
     gene            complement(35132..36802)
                     /locus_tag="PLY83_01540"
     CDS             complement(35132..36802)
                     /locus_tag="PLY83_01540"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOV28750.1"
                     /translation="MIAGIEPGLLRHWRIDRTTADLSREIGCSPLAGAILQMRGVPIE
                     EDPRDLFHPDLDVLMERVNLGPGVEAAKEVFDRMKAGTRLVVYGDYDVDGVSATTLAA
                     RLFSFRDVDVRYFIPHRHQEGYGLHERIVRKIADWGCDVLVAVDCGTSSENALAAARD
                     LGMEAVVFDHHLPREKRQAETGAVMVNPQFAGNPEAKALCGTAVLWAWAWKSKAFERN
                     WLLENLGLVALATVADCVPLGPLNRALVREGLQKIREGKEPGLEALSNQLGLDLGALD
                     CESLAMKVIPCLNAPGRLDTADLAVKVLSGESPLEKAVGDLVVLNRKRQGLTARIMEE
                     ARPLFEGKAGLVAEHDDWPVGVLSGVASRICSEMGCPVVLAAPVNAGLVRGTLRVPKG
                     ADAVSVLERLAPSLKEWGGHRQAAGFSVARQDWPSVRDSLKILLAGLRTCDSDCLDVL
                     DLHPEDLSLEALEEIDRLGPFGFGNPGPLFYVQRGAENRIVSHGKDGLHARIEFSKAS
                     IVAFRSPELLKDAGSVEGWVYRARKGFWRGRARVDLFLEKPVVRREKG"
     gene            complement(36799..37161)
                     /locus_tag="PLY83_01545"
     CDS             complement(36799..37161)
                     /locus_tag="PLY83_01545"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HOV28751.1"
                     /translation="MKSYALAILLAMLVAAGYAFQNTGDVVVRFIFWEKTLPQGLWDI
                     LLFAAGGLLMWIVSLASLIEVRSALRRKMKEQEERIRSLEQERQQLLEALKGSTAEVA
                     RAVEANPDPEGSASGSPQ"
     gene            complement(37158..38639)
                     /locus_tag="PLY83_01550"
     CDS             complement(37158..38639)
                     /locus_tag="PLY83_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOV28752.1"
                     /translation="MVRQSIFDQARGIVSARLLDGLIQAIEDGREKNLAALFRIISAL
                     APSKYHRTGFEKLAFMVETDHPFIRVFKRTFSELHPNVRKKFIANFLVNFIVLGRGIR
                     DREEKARGIHIPNFLVISPTMRCNLHCRGCYASEYRGGEELSFEELDRILTEAKQLGM
                     YFFTFSGGECFIRKDLPDLWEKHGDCFFQVYTNGTLLDEPMVERLVRLGNVAPMVSIE
                     GSPKSTDARRGPGMHDRIMETYARLKKAGVLFGFSATYTRENAEEIASEEFIDSMISA
                     GCLAGWFFQYIPTGLNPDPSLMATPEQRARLHEKVEQWRREKPIFMGDFWNDGPYVDG
                     CMAGGERYVHIIANGDVEPCVFVHFAVDNIHGKKLTEVFQSSFFQAIREAQPYDDDNL
                     LRPCLIIDHPHVLREIVERTHAHPTHPGAESILQEMAGDLDRYAEEMKAIYDPIWENS
                     GRDRYLQSLQNEDKPEVHARIGRRASRQESLSGSGAATKEGTM"
     gene            complement(38642..38953)
                     /locus_tag="PLY83_01555"
     CDS             complement(38642..38953)
                     /locus_tag="PLY83_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV28753.1"
                     /translation="METVLQLLMEGVGTGTAALAIMRDNMSSLVQKGIISQADLDGLL
                     QEVERSLEARRKTLIFGFQKELQRLLAHLPLATKEDLQAFELRFRILEERIERLEAKG
                     A"
     gene            complement(39061..39933)
                     /gene="secF"
                     /locus_tag="PLY83_01560"
     CDS             complement(39061..39933)
                     /gene="secF"
                     /locus_tag="PLY83_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOV28754.1"
                     /translation="MKFLHDRQFDFMAFRKIAMGISLFLVVGSLFLIFFRGLNLGIDY
                     TGGTLLQVEFPAPVSVGQVRETLKSVGQEQSVIQSYSDRGMIIRLKAGEETVGQKARE
                     ALRKQFGEIQVLRLEKVGPVVGKELSRGALIALGLALVGILLYVSFRFQFRFAAVSVI
                     ALAHDAIIVTGAFALTGKEISLTFIAAILTVVGYSINDTIVVLDRVRENWGRLRQLGI
                     ERLLNDSVNQTLSRTINTSLTTLLPVIALFLFGGPVIADFSFALLVGLIVGTYSSIFI
                     ACALLCEWMQRSPR"
     gene            complement(39947..41338)
                     /gene="secD"
                     /locus_tag="PLY83_01565"
     CDS             complement(39947..41338)
                     /gene="secD"
                     /locus_tag="PLY83_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOV28755.1"
                     /translation="MLKRDRWRLIIVAAVIVAALLAVFPIQGRVRLGLDLKGGAHIVL
                     LAKDTPENPLTDDSIERLIAVLRNRVDQYGVTEPVIQRQGKDRVILDLPGIQDPEAAL
                     ELIGRTALLEFKQVLQSTGAVPPAPNRPNYASEEDFLAAQARWQKAREEVDALANELK
                     ATAARTPGTTVGRDRDGRAYLLGKTYVVGKDLKDARTGFDSLSRPMVSLKFSPEGTRL
                     FDEATASNVGRQIAIVLDGEVISAPVVQERISGGEAQITGRFTVDEANRLAIMLRAGA
                     LPVPVEVAENRTVGPTLGSDSIRSGLRAGIIGAVLVLVFMLVYYRFLGIAANLALCVA
                     LLLVFAGLITFRSTLTLPGIAGIVLTIGMAVDGNILIYERMKEELRSGKTRLAALDAG
                     FRKALMTILDSNLTTLIAAMILFYFGSGPIRGFAVTLSIGIVSSVFCNVVVTRAILQL
                     LLDFSRKKALGSN"
     gene            complement(41450..41752)
                     /gene="yajC"
                     /locus_tag="PLY83_01570"
     CDS             complement(41450..41752)
                     /gene="yajC"
                     /locus_tag="PLY83_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOV28756.1"
                     /translation="MGMLLPLALFVVIFYFLIIRPQKKRQRQHQEMLASIRKGDQVVT
                     AGGFFGIVRDTKEDSFILEIADGVKARILKSSIQYKRTEEAGKKEETPSVEGIPEA"
     gene            complement(41838..42464)
                     /locus_tag="PLY83_01575"
     CDS             complement(41838..42464)
                     /locus_tag="PLY83_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOV28757.1"
                     /translation="MKVAEPTIERLVQYYRLLELLHEEGRRVVSSQEIGEMLSLKASQ
                     VRKDLSYFGEIGKRGVGYHVEKLYEHIKSILSTPRLWRIALVGVGRLGEALLGHKAFQ
                     SSKFKVLALFDVDPAKVGRSILGIPCYPIEKITDVLREQQVEILILTVPAAAAQGCVD
                     RAVAAGTIRGILSFAQTAVIVPKSVLVYHVDIATELEKLLFFLKQLER"
     gene            complement(42501..42881)
                     /locus_tag="PLY83_01580"
     CDS             complement(42501..42881)
                     /locus_tag="PLY83_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002886926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOV28758.1"
                     /translation="MKKIISTENAPAAIGPYSQGVWAGDFLFLSGQIPIDPKTGSLVE
                     GDAAAQAEQVLKNVKAILESQGLTLRHVVKATVFSTDMGTFAAVNEVYGRFFSEEPPA
                     RSFVEVAALPKGALVEIEVVASRQ"
     gene            complement(42909..43196)
                     /locus_tag="PLY83_01585"
     CDS             complement(42909..43196)
                     /locus_tag="PLY83_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HOV28759.1"
                     /translation="MRIDLFLKLSRLVKRRATAREMVEMGAVKLNGKTVKPSAEVRSA
                     DRIEIAYPRRVLLVEVLSGESRIPRGENEMFRVLEDRCVKGEGLWEKGLDS"
     gene            complement(43266..>43462)
                     /locus_tag="PLY83_01590"
     CDS             complement(43266..>43462)
                     /locus_tag="PLY83_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HOV28760.1"
                     /translation="EDLTDREREVLHLRFGLEDGHAYTLEEVGKRFGVTRERIRQIEA
                     KALRKLRHPSRSRKLRDFLE"
BASE COUNT         8423 a        14116 c        11870 g         9053 t
ORIGIN      
        1 cgctcggtgg tcgtggtctt gcccgcatcg atgtgggcgg cgattccgat gtttcggata
       61 tttttcaaat cgagtttcgg catgatttca ggctccccgg cgccggcagt ccctgcgctg
      121 cttcatcacg gatctaccag cggtagtgcg caaacgccct gttggcctcc gccatcttgt
      181 gcgtatcttc gcgctttttc atggacgccc cttcaccctt gtacgcgtct gcaaactccc
      241 gcgcgagacg ctctaccatc ggcatcccct tcttggcccg ggcatactgg atgatccagc
      301 ggatggcaag ggcttccgcc cgctcgggcc cgacctctac cggcacctgg taggtggcgc
      361 cgccgactcg tctcggcttg acctccacaa ccggcttcac gttgtccatg gccttccgga
      421 aaacctcgtc gggctgcgta ttcagccgct tcgctgaaag ctccagggcc ccgtagacga
      481 tcttctcggc cgtgctcttc ttcccgcaat acatgacgca gtttacgaac ttcgtaaggg
      541 cgggtattcc gaaaaccgga tccgccgcct gtgtcctttt tctaacgtgc ccctttcgtg
      601 gcatgggcta aagcctccct ttccggaaaa aactatttcg ggcgccgtgc cccgtatttc
      661 gaacggctct gccggcggcc ttcaaccccg ccacagtcca gcgttccgcg gatgatgtga
      721 taacgcacgc ctggcaggtc cttgacacgc ccgccacgga caagaaccac cgagtgctcc
      781 tgcaggttat gcccgacacc cgggatatag gcggtcactt cgatcccgtt tgtgaggcgg
      841 acacgggcga ccttcctcaa agcggaattc ggcttcttcg gtgtgaccgt gtacactcgc
      901 gtgcacactc cccttcggga agggttcccc tgaagggcgg gagcgctgga gcgttctctc
      961 ttttcttccc gcccgaggcg gatcaactga ctgatcgttg gcacacaaac tcctcctttc
     1021 cctactcgat attcttcaca ttctccccga gcaggcctgc ggcggctgcc tttcgagaaa
     1081 tggcgcaggc acggcccagt atttccatgc tctcgcacca ctccacctcg agatttcgct
     1141 tcccggcttc ctcaaggagt tccttgaccc gatcctcttc agcgtcacgc gcgagaaaaa
     1201 gcttctgcaa ctgccccgac cgaatcttcc ggaaaacctg gcgaacaccg accactcgac
     1261 ggtccgatgc cagctcatcc aaaggcacag caatcccccc tgtgcggacg caccgagaca
     1321 taatatcaac cggggaagcc cctgtcaaga aaacggggcc tccccggttc ggggtcgttt
     1381 ttgttgccgc ttttctttcc cggtttacgc cttttcctgc atctcgacgt ggcggtaagc
     1441 ttccgctccg gtcccggccg gaatgaggtg cccgatgatc acgttctcct tgagtccctt
     1501 aagggtgtcg acgtctccgc ggacagcggc ctgcgccagg atctgcgccg tctgctggaa
     1561 cgaggcagcg ctcatgaagc tgtcggttgc cagggcggcc ttggtcactc catggacaac
     1621 gggacgccat tgcggctcct ccctcatcct ccgaacgaac gaaacccgct ggatcaggcg
     1681 cttggtttca atggagctgg agatcggcct gacgaccagt tcctggaatt ctccctgaac
     1741 gaggcggtcg atgacctcgc ggctgatctc cgtgcctgcg tccgcaagca gttcccctct
     1801 cgcgttgcag atcggttcca ggaggacctt cccgtaggcg atatcgctta ccacctggcc
     1861 caggagtttt tcgcgcgtca ggatttcctg cgtctccccc tcgagttcaa ccgccgcgat
     1921 ttgtccttcc accagccctc ggacgaccct tccgtccagg atccggccaa cgtcgtttac
     1981 cgggtttccc tgggggttga tggcctgctt gatcggccgg ctgaaaaact tctcgatcag
     2041 gtagtggttc agggcatctc cgatgttatg cctggccggg gcccgccaaa tcttcacaac
     2101 cgatgcgttg gcctccttca gccgttcgag cgcttccggc gtgagcgccc ggtcgcggct
     2161 caggaacacc ttccctccgg acacgacatc ctcggcgagg taggctccgc gctccaggct
     2221 ggccagggtt tcggtatccc tcacgcagag gatctgcggc tttcctcgcg taattgccag
     2281 aagatcctgg agctccagcg ccgatttggg ttcaatcacc gtgttgtttt ccagggagac
     2341 cggcgtgatg agctccaggc cttccatttc ccgacggaaa gtcgcttccc cgaggatgac
     2401 ccgaaccagg ccctctccat cctccacggt gatctccctc gcggctgatc ccggctggag
     2461 cagcctctcg atcgtgtccg catcgagggg ctttcctttc agggatagga catggtcgat
     2521 cccgctctgt ccctcgatgt cccgcaatgt cttgccctgc aggaaatcca gggcttcgcc
     2581 gagacacctc tcgttttcct tccggatccg atccagctcc tggtccaggt catcgagcca
     2641 tgccagttcg ttcgcaacga aggagctgtc cccctcctcg gtgatccgga cgcggttgac
     2701 cggcgccact ttcctgagga tcacctcgat atgcttgttg ttgatcgaaa caccctggga
     2761 gaagtagacc tcctggatgc cgtcaacgag gtatttctgc actgcttcca gtccctcaac
     2821 ctcgagcagc tgctgcggat cgatataccc ttcggtaagg cactggcctt tcttcacgag
     2881 ctggccttcc tcgacgagca ggttttgcga agcgggaacc gtgcagatga ttttctcccc
     2941 ctcgtcttcc cccttgccga tgatgacctt ccgcttccct tccatctcgc ggatttcaag
     3001 cacccggccg tccgcctcga caagctgggc caccttcttg ggccggcgga cctcgaaaag
     3061 ctgctcgatt ctcgggagcc cctgggtgat gtcttcgccc gtcaggcgca cgccaccggt
     3121 atggaaggtc cgcatcgtca gctgggttcc cggttcaccg atcgattggg cggccaccac
     3181 gccaaccgct tccccgatgg ccacggtttt cctcgtcgcg aggtcccttc cgtagcactt
     3241 gcggcagata ccgttccgaa gtccgcaggt catcgggctg cgaacccaaa ccgattcgat
     3301 tcccgcattc tcgattttca aagcggtttc ctggctgatt tcgtccccgc cccggacaag
     3361 cacatccccc gaggaggggt cctcgatgtc ataaagcgca gtccttccgg aaatccggtc
     3421 cgcaagcgta acgacggtct tcccgtcctg gatcagggcg ctgatccgcg ttcccttgtc
     3481 tgtgccgcaa tcctcttcgg tgatgatcag gtcctgggcc acgtcgacca ggcgcctcgt
     3541 gaggtagccc gacttggccg tccgcaaagc cgtgtcggcc aaccctttcc gggccccgtg
     3601 agtcgagatg aagtactcga gcatgttcag cccgtcacgg aaattggcca caatcgggta
     3661 gtcgatgatg cggccggacg gatccgccat aaggccccgg atcccggcca tctgggcaac
     3721 ctggttcttg cttccgcgag ccccggaatc gatcatcatc cggaggggat tcagctggtc
     3781 catgttggca acgatatcgt cggcgatctg ccttgccgcc tccgaccaga ggatttcctt
     3841 ctgcctcagg tattcttcct ccgtcaggac ccccatgccg tactgggcgg aaagctcttc
     3901 ctcccgtttc atggtttcat cgacggtggt cttcttgctg ggcgggatga ccgcgcagtc
     3961 aagtccgaag ctgatgccgc tccgggtgga ccactggtag ccgaggatct tgatcgcatc
     4021 gagcatgtcg acgatcgccg cctgcccgac cttgtcgtag gccttgtcga gaaggcttcc
     4081 gaggtccttc ttcccgattt gccggttgat gtaccgaaga tcagggtgaa ggacgctgtt
     4141 gaatagtgca cgtccgggag ttgtttcgat ccactcccct ttccacagca ttttgacccg
     4201 ggtgttcggg tgaacgacgc cgtggtcgat cgccgtcagg gcatcatcga tatcgcggaa
     4261 ggccatgcct tcccctttca gcccgtccct catggccgtc aggtagaaaa tgccgaggac
     4321 gatgtcctgc gtaggcgtca cgacgggcct gccgctggcc ggggaaagaa ggttattggc
     4381 cgacagcatc aggatgcggg cctcggcctg ggcctcgagc gacaggggaa cgtggacggc
     4441 catctggtcc ccgtcgaagt cggcgttgaa ggcggtgcag acgagcggcg ggagacgaat
     4501 ggccttccct tccatcagaa ccggttcgaa agcctgaatc ccgaggcgat ggagggtcgg
     4561 ggcccggttc agcatgaccg ggtgatcctt gatgatctct tccaagatcg cccagatttc
     4621 atctcgcccg cgctcgatga gacgcctggc gctcttgacg ttcggggcca gcccccgctc
     4681 caccagcttg tgcatgacga aaggcttgaa aagctcgagg gccatctgct tcggcaggcc
     4741 gcactggtag atcctgagct tggggccaat cacgatgacc gaccgcccgg agtagtcgac
     4801 gcgcttgccc aggaggttct ggcggaaacg tcccttcttg ccccgcagga ggtcgctcaa
     4861 gcttttgagc gtgcggtttc ccgcacccag gacggccttg cccatccgtc cgttgtcgat
     4921 gagggcgtcc accgactcct gcagcatcct tttttcgttg cggacgatga tctcaggagc
     4981 cctcagttcc tgcaatttcc gaagccggtt gttccggttg atcacccggc ggtacaggtc
     5041 attcaggtcg gaggtggcga accgtcctcc ctccagctga accatcggac gcagatccgg
     5101 gggaatcacc gggaggacct ccaggatcat ccactggggc ttgctggcgc ttttacggaa
     5161 gtcctcgaca acctgcaggc gcttgatcag cttccgtttt ttctggcccg tggtttcgga
     5221 aatctcttcc cgaagggagg cggccatttc gtccaggtcc agcttgttca aaagctgctg
     5281 gactccttcg gcaccgatcc cggcggaaaa ttcccgatcg taggctgcac agaggcgttg
     5341 ctccctttcg agaatgattt ctccgcgggc gaagggagat tctcccccgt cgatgacgag
     5401 gtaacaggga tcctcgatgg tcgcggtttc ctgctgggcc tggaaacggc caggatattt
     5461 ctggttgtaa agctggacct cgctgagggc cagcacgtcg ttcttctgga acggcaaatg
     5521 ggcgacggcc gtgacgatga acgcttcctg gttgccgaca acggcgcgct ggattccggg
     5581 gaatgcctcc actccccctc gtgcctccag ttcgctggcg gacaggatct cgcccggtcc
     5641 ttccgagccc tcctgttcga tccggaatgc cggttcgacc ttgaagatct catccccaaa
     5701 atcggccttg aaacgggcaa cctgttgggc ggaaacgagg tccccttcgt ccaggggaac
     5761 gtcgtccacc tggatcaccc ggtacgcctc ctccgccttg aacttcgggt cgtagtggga
     5821 gtggattctc tcttcagacg catcgatcag gtcgccgcgg cgggcaaggt ctgtccttcg
     5881 cccttccatg acgaccttga acacaggttt tctcctccgg atcggggcaa agtagacgac
     5941 tttctccagg tcctttgttg ccgtccccag gaggaggctc aggcggctgg gaatgcctct
     6001 caggtaccag atatggacga ccggggccgc tagctcgatg tgtcccatcc gttcccgccg
     6061 gacccggttg tccgtcacct ccacgccaca acggtcgcaa aggatcccct tgtactttgg
     6121 accgctccgc ttgtatttcc cgcaagcgca ctcgtagctg cgcgttgggc cgaagatcct
     6181 ctcgcagaac agcccgtcct tttccggacg gagggtccgg taattgatcg tctcgggttt
     6241 cttgacttcc ccgctggaaa gttcacgaat ccgttccgtg ctcgcgagct tgatgcggac
     6301 tccgacgatt tcgcggcggc tcatttatgc atcctccccc tcgcgggtca aatcctgttc
     6361 atccggttcc tcttcttctt caaaagagga gtcgagttct ttcgggctta caaaaagcat
     6421 ctcctccgta acgccggccg cagagggtgg cagcaggtcc tcgcccgatt ccaaatcgac
     6481 aatcgccggc cgcggcctcg gcgtccccgg ggctcccatt tcctcgtcct cgtcctccaa
     6541 gaggatttcc ccgaccgtcc cgtcgtcgta catgatctcg acatccagcg ccaggccctg
     6601 aagttccttg acgagtaccc ggaagctttc cggcaccccg ggcttcgtca ggttctgccc
     6661 cttcacgatc cgctcatagg ttttcaggcg gccgcggatg tcgtcggatt tcaccgtcag
     6721 gatctcctga aggatgtgcg cggccccgta cccctcaagg gcccagacct ccatctcccc
     6781 gaaccgctgg ccgccgaact gggcttttcc tcccagcggc tgctgggtga tcaggctgta
     6841 aggaccgatg gaacgggcat ggatcttgtc gtccaccagg tggatcagtt tcagcatgta
     6901 catgtagccg acggtaacct tgttttccat cgcttccccg gtcctgccgt cgtagagcgt
     6961 gatgcggccg tcctcggcca tttcgggata ggggccctcg gccagcttcc tgacgccatc
     7021 gaagatgtcc tgctccgtcg cgccctcgaa aaccggggtc gaaaccttcc agccgttgtg
     7081 gacagcaaca aaccccatga ccgtctccat gacctgtccg aggttcatcc ggctcggtac
     7141 gccgagtggg ctgaggacca catcgaccgg cgtgccatcc gggaggtagg gcatgtcctc
     7201 gacagggagg attttcgaaa cgaccccctt gttcccgtga cgacccgcga tcttgtctcc
     7261 ttccgagatt ttccggagct gggccacgta gacctttacc acctcgttca ctccggggct
     7321 cagatcatcg ctgttctggt cgatcgtcag gcgcttgacg gaaaccacct tgccgccctc
     7381 tccgttcggg acccgcatgg acgtgtcccg gacctcgcga gccttctccc cgaagatggc
     7441 ccttaaaagc ttctcttccg gggactggtc agattctcct tttggagtca ccttgccaac
     7501 gaggatgtcg ccggcccgca cttcggcccc gacccggaca atgccgttct cgtcgagatt
     7561 cttgagggcg tcttccccga cattggggat gtcccgggtg atctcctcgg gtcccagctt
     7621 ggtatccctt gcctcgactt catactcctc gatgtgaatg gaggaatagt gatcctcctg
     7681 cacgagcctc tcgttcagga ggatcgcgtc ctcgaagttg tagccctccc aggagacgaa
     7741 ggcaacgagg acattgcgtc cgagggccag ttccccgtga tccacggact ggccatcggc
     7801 gatgatttcc ccggcctcga ccctatcgcc gttcgaaacg atggggcgat ggtggatcac
     7861 ggtgccctgg ttggatcgcc ggaatttcgt caggttgtaa gaatccagcg aaccgctctc
     7921 ggtgcggatc tcaatccggc ttgcatcgac gaattccacg gtccccgagc gccgcgccct
     7981 tacgcagcag cctgaatcct tcgcgatatg gtgctcgatt cccgacccga tgatgggagc
     8041 ctgcggaagc accaggggca cggcctgccg ctgcatgttc gatcccatca gggcacggtt
     8101 cgcgtcatcg tgctccagga agggaatcag ggccgtcgaa atcgaaacga tctgtttcgg
     8161 ggaaacatcc aggaaattga ttttctcggg ttcgaccgtt tcgatctccc ccctgaaacg
     8221 gacgtggaca tcggggccca agatccggcc ctgctcgtcc atcggggtgt tcgccctccc
     8281 cacgaagaat tcgtcttctt cgtccgccga aaggtacacg atctcgtcgg tcacccgccc
     8341 ctcaacgacg cgcctcttcg gggtcacgag aaagccgtag tcgttgattc gagcgtaggt
     8401 tgcaagagac gtcacgagcc cgatgttcgg gccttccggc gtttcgatcg ggcaaacgcg
     8461 tccgtagtgg gtgtagtgaa cgtcccgcgc ctcgaagccg gtccgttccc ggctgaggcc
     8521 accgggcccc agcgcggaga gcctgcgcct gtgcgtcagc tcggccagcg ggtttgtctg
     8581 gtccatgaac tgggaaagct gggccgaacc gaaaaattcc ctcagggagg cggaaatcgg
     8641 cctgacgttg atcaggtcct ttgccgtagc gctgctcagg tccggaagcg ttgtcatcct
     8701 ttccttggcg atccgctcca tcctcagaag gccgatgcgc acctggttct gcagaagctc
     8761 gccaacggcc cggacccgcc tgttccccag atgatcgatg tcgtcttcct tttcctctcc
     8821 gtttcgaagc tgcaggatcc ccttgatgat ggcaacgatg tcatcgacgg tcaaaagcct
     8881 ctggctctcg gggatatcaa gccccaaccg cttgttgagc ttgtagcgcc ccacacggcc
     8941 gaggttgtac cggcgggtgt caaaaaagag cccgtagacg tattccttgg cgttttccat
     9001 ccgggcgggt tcgttcgggc gaaggcggcg gaaaagctcc aggaccgcgt cctcccccga
     9061 gcgggagtta tcccgttcca gggttgccgc gatggcattg tccacatccc agacccaaac
     9121 ggtcgtcacg cccatctcga agaggagctc aaggtgttcc ttcgtcaggc ggtcatttcg
     9181 ccggatcagg acctggcctt ccttgtccag gatggcttcg gccaggaggc ggcctcgcgc
     9241 ctcgtcgtgg agttccacga gatccacctg catgggcttc gccccgaaca gcttcaggat
     9301 ttcctcgtcg gtcgtcgccc cgaatgcctt gagaagcatc gtcacgggaa tcttcttgcg
     9361 gttgtcgatg ttgacggaaa ggaggtctcc cggcgtcaga tcgaactcaa gccaggcgcc
     9421 ccggtccggg ataacttttg cagcatagga ttcccccccg gggacggtcg tgtcggaact
     9481 gaaatacaca ccggcggacc gtgcgagctg gttcaccacg acccgttcgg tcccgttgac
     9541 aatgaacgtt ccccgctccg tcattacggg aaaatcaccg aggtagattt cttcttcctt
     9601 gatctcaccg tttcgacggt taaccagccg gatcgtcgcc ctgacgggca tggaccaggt
     9661 caggtctctc tgccgggctt cttcctcggt aatcggggac tcatcgacaa aatagcggac
     9721 aaactcgagc gcaaacgatc cgtcgtaact ctcgatgggg aaaatttcct ccaggatttc
     9781 ctggagtccc tgaggttccc gggaatccgc ctccttttcg gcctggaaaa accagcggta
     9841 ggaatttttc tgtacctcga cgagatccgg taagccaatg agatccttcg ttctcccaaa
     9901 aacgcggcgc tgaatgtgtt ccttcgaggg ggcaaaatct gtcatgggag ttacgacctc
     9961 ccttttgagg tttgtgggga ctatcctaga cgtgacgaag gggcataata gcaaaaaggt
    10021 cgcgcaatgt caacagacag gcgcgacctc gctgacccat tctctgggaa tcatttttat
    10081 ggtggtgtcg agggggggag ttgaaccccc acaggctaat gcccactaga gcctgagtct
    10141 agcgcgtcta ccaattccgc caccccgaca tcacgatcga caaacgggat tttagcaacg
    10201 tgcgcccttt ttggcaaggg ctttccctct attgggggtt gaacgatcca cccgaggctc
    10261 gcctgagccg gtcgcgcaag gcgagcccga catgggcttc ctccggccaa tccgccacga
    10321 tgactttcgc tcccgccttc tccatttccc gaaacacgga aaacaggcat cgggcgtagg
    10381 tttccacatc gggaaagaga cggacgcagc cgaacgggac aaccggggga cgaatcccga
    10441 tgtaggccct ttcaccgggt tcccatgcgg cgacttttgc ccacacggac tcgtcctccc
    10501 gcaggaacag aggaaccttc ggggcataat gccggtagcg ggtgccaggc gagcgcctgg
    10561 aaaccggcgc tccggctccg gcctgcaggg ggcagtatcc gaaaaaatcc gccaaggatt
    10621 ccaggctcgt tcctccgggc ctcaacagga ccgcccgttc tccggtaata tccagaacgg
    10681 tcgattcgac cccgaaagcg gtcgatcctc catccaggat gatgtccacc ttttccccga
    10741 gatcttcttc gacagcggcc gcatccgtcg ggctaggccg gccgctcagg ttggcgctgg
    10801 gggccgctac cgggagatcc gccagtcgga tcagctcgag ggcgatcctt tgggcaggca
    10861 ttctcaccgc caccgtttta agcccggccg tcacctcttc cggaacgacg ctcctggccg
    10921 gcatgacaag cgtcagggga ccgggccaga accgtttcat gaggtctatc cccaattccg
    10981 gtacaaaggc cactttttcc gctccggcgg gctcggacac gtgaacgatc agggggttgt
    11041 cagaagggcg ccccttggcc tcgaagattt tccggacagc ccttttgtcg agcgcgttgg
    11101 ccccaagccc gtaaaccgtt tctgtcggga aaacgacgag tgctccctcc ctgagaatcc
    11161 gggccgcttc tctcaggatt tccatgtcac gggccgaaag ggcctccgaa accatgaccc
    11221 tcatgacgca ggtcccccgt caccgaaccg ttccagaaag gcctgccgga attctgggaa
    11281 tgtccctttc acgatcgatt cccgcgcctt tttcatcagg cggatcaaaa agtgaaggtt
    11341 atgccaggaa cagagcctcg ccgccaggat ctccccagcc ttgtacaagt gccggatgta
    11401 ggcccgtgtg aagcgttggc agaccatgca gtcgcattca ggatcgatcg gcgagaaatc
    11461 ccgggcgaag gtggcgttct tcaggttcag tcgacccccc gaaaagaaga ccgtcccgtt
    11521 ccttccggtt ctcgtgggca gaacgcaatc gaacatatcg acccccctgg aaaccccctc
    11581 caggaggttc gaaggcaacc cgacccccat caggtaccga gggcgatctt ccggcaaaac
    11641 tgggttcagc acatccagga tccgatacat ttccgagtgg ggctctccaa ctgaaagccc
    11701 cccgatcgcg tagccgtcga aaccgatctc catcagggcc tccgccgaac gccgccgatg
    11761 ctcttcaaaa acggacccct ggacgatgcc gaataaggcc tgatcctctt tccggtgaac
    11821 cgcccggcac cgctgggccc atcgcgtcgt cctcaaaacg gcctccagcg aggcagactc
    11881 gctcgccggg tacgggacgc actcgtcgaa acacatggcg atgtcactcc cgagatcctc
    11941 ctgaatctcc atggctcttt ccggagtcat gaaatgggaa ctgccgtcca ggtgagaacg
    12001 gaacgtcacg ccctcatcgg tgacttttct aaggtccgat agggagaaga cctggaagcc
    12061 tccgctgtcg gtcagaatcg cacccttcca gtccatgaag cggtgcagtc ccccggcttc
    12121 ccggataagc tttgaccccg gcctcaggaa aaggtgatac gtgtttgaaa gaatgattct
    12181 ggctcccatc cccgacagct cgtcgggagt catggccttg accgtcgcct gggttcccac
    12241 cggcatgaac accggcgtct cgataggccc gtgcggggtc ctgaggaccc ctgcgcgtgc
    12301 gcccgtcacc gggcaccgcg cctcgaggcg gaattcaaac atttccgtcc ccccatccga
    12361 aaagccgttg ggcatttcgc cagacctgct cggcaaggac ttctagggaa acctttcgaa
    12421 cctccgccag agtttcataa acagcggcaa cataggccgg ctggttgcgc ctgcccctgt
    12481 agggctgcgg agcgaggtac ggggaatccg tttcgcaaag gaggcggtct tctggaacct
    12541 ttgcggccac ctcccgtagc gccgctgccc ggggaaaggt cagcggtccc gcgaaagaaa
    12601 gaaaaaaccc catttccagc gcttcggccg cgtgacgcca ctcccccgcg aaacagtgga
    12661 tcactcccgt acagccccgg gcggcttcat cacgaaggat tccgatcgct tcatcgtagg
    12721 catcccggac gtgaaccacc agcggcttcc tgacccgttc cgccagggcg atatgccggc
    12781 ggaaagactc ctcctgcaca tccctcggcg ataggttgta gtgaaggtcg agcccggttt
    12841 ctccgatggc gacgactcta gggtcggctg cccaggcctg gaggctttcc ggcaagcccg
    12901 ccggcgggca ggttttcgca tcatggggat gaacccccac cgcggcataa acgccttccg
    12961 cggaatgggc tttcgctagg gccagggcct ttccgctcga aggttcgtca aaaccggcca
    13021 ccagcgttcg ctctacacgg gcttccctgg ccaagcgaag gatcaggtcc aggtcttcag
    13081 caaattcttc catgttgaga tggcaatggc tgtcaagaag tctcatcagg gcctccaaaa
    13141 ttgaatcgcg ggcgatcggg cgcccgcgat tttcagactg gcacggttcc gggcgggatg
    13201 ggcgaaccgc ctattagtgg atccggcttc ccggtgttat gggcctgtcg agcgtcagaa
    13261 gggccagttc ctttccatcc gggctttccg ccgccagcag catcccgttg ctcacaaccc
    13321 cccgtagttt tgcgggcttc aggttgcaga tgacgacgat atcccgcccc acaagttccg
    13381 cgggttcgta aaactcccga atgcccgaaa cgatcgttct tctctcgtag ccaaggtcaa
    13441 ggttgagctt gtacagcttg ctggcgttcg ggaccggttc gacgctcagg acccgggcgg
    13501 ttctcagctc gatcttccgg aattcctcca ccccgacttc cgcctcgtgt tctcccggat
    13561 cggggagcag gttctttttc gcttcccgtt ccgccttggc ttcctcccag gctttcagat
    13621 cgattcgcgg gaaaaggacc tcgtccctcc ggatggtcgt cccttccgga aggaagcccc
    13681 aagggtaatc ctcgaacagg gcgctggcgg gatcccctgc cagcccgagc tgtctccaca
    13741 tgcggcaagc cgtttcgggc atgaaggggg ccaccatgag ggcgatctgg atcaaggcct
    13801 ggtacagcgt tccaaggact gagtccagcc tggggtcatt ctccttccca agcttccagg
    13861 gagccgtttc gtcgatgtac ttgttcatcc gggaaatgaa cgaccaaatg gccttcaggg
    13921 cttcgtcgaa agcgaactcg gacatccggg cacgaacggt ctcgaatgtc gaagtggcca
    13981 gacgggccat ctcgagatcg agtgggtcca caggtccgca ggccgtcacg gtgcagccct
    14041 tgaaccgctc gatcatctga agcgttcggt tcagaaggtt gcccagatcg ttcgccagat
    14101 ccgagttgat ccgcttgaca agggcgatct cggaaaagtc cccgtcgagt ccgaagggga
    14161 cctcgcgaag aaggaagtac cggaacgcat ccttcccgta aaggtccacc atctcgaacg
    14221 gatccaccac attccccttg gacttggaca ttttctcccc ctcgacggtc caccagccgt
    14281 gggaaaacac ctggcgcggc gggttgaccc ccagggcgag aagcatcgcg ggccagacga
    14341 cacagtggaa ccggatgatg tctttcccga caaggtgccg gacgtgcggc cagtattgct
    14401 tccatttcgc ttcttcgtcg gggtatccgc agaccgtcag gtaattgatc agggcatcga
    14461 accagacgta gatgacgtgg cggtcatctc ccgggaccgg gatcccccac ttcagcgtcg
    14521 tccgggagat ggactggtcc ttgagtccgc tcctgatgaa gctgacgatc tcgttgtacc
    14581 gggtccgggg caggatggcg tccgggtttt cctcgtagaa gcgcagcagg ggctcctggt
    14641 acttggacat gcggaagaag tagctttcct cggtcatctt ttccagcggt ctcttgcaat
    14701 cggggcaagt cttgccttca cccatctggg cttcaggaac gtaggtttcg caggggacgc
    14761 aataccatcc ttcgtaggtc cccttgtaaa cgtccccctg ggcgaggagt ttttcgaaaa
    14821 ccgccgtcac gaccttgaaa tgccgctctt cggtggtgcg gataaagtca tcgtttgaaa
    14881 taccgagggc ttcccagagc tttttgaaat tttcaaccgt ctggtcggca agctgctgag
    14941 ccgtaagtcc ccggctctcc gcggcctgct ggatcttctg cccgtgttca tcggttcccg
    15001 tagcgaacag caccggttgt cccatcatcc ggtgaaaccg tgcgctgaca tccgccgcga
    15061 tcgtggtata ggcatgcccg atgtggggaa catcgttgac gtaatagatg ggggtcgtga
    15121 tgtaaaaagg ttccctgttc atcctttaat cctccttttc gcgctcccct gaccgctcag
    15181 gggggattcc ccctgttttg tggcattttc gatcaggtag gccttgatcc gatttttcgg
    15241 aataccatat cgctcgtaaa ccattttgac aacatccttc ccagaaagcc cctcctcgac
    15301 agccgcgtcg accagcttct cccaggattc cgaagggcaa tcctcggttt tttcgggatc
    15361 ctgggaggcc cccgccacca ccagcaccat ttcccccttc atgggagtcc ggacgctttc
    15421 cctcaggtcg tcgaggttca tccgcaggac ttcctcgtgg accttgctga gttcccggac
    15481 caaggcgcaa gggcggttcc cgagaacgcg ggcacaatcg tccacttcat cggaaaggcg
    15541 atggggagaa acgtagaaaa caagcgtaca ggggacatcc ttcaaggcga gaagccgcct
    15601 ctgcctgtcc ccttttctgg ggggaaggaa accagcaaac tggaaggtct ggatcggcag
    15661 gcctgaaagc acgagcgctg ggacaaaagc cgtcgctcca gggagaactt caacctcgat
    15721 cccctcccgg atggcttcgt tcacgaggaa agctccgggg tccgagatgc caggcgtccc
    15781 tgcatccgtg accagcgcaa cccgttttcc ctcccggagc gccttcagaa ggaaaggcgc
    15841 tttttctctt tcattgtgct cgtgggaaga aaccagcggc gcgcgaatac ccaggtgatt
    15901 caacaggcga agcgttctcc tcgtatcctc gcaggcgatc cagtccgcct cccggagaac
    15961 ccggatcgcc ctcagggtga tgtcctcgag gttccccacc ggcgtcggga caacaatcag
    16021 aggcatggcg agctctccac gcggtaggcg gaaagaagtt cgcgggtgta gtttccgctt
    16081 tcgtctgtga tgaagagggg aggatcgacg acaatgccac ccctgccctg cttcatcgca
    16141 acaaggagaa aggtcgaggc ttccttcccg gggcccggat gaacgaaacg gatccgtttg
    16201 ggctcaagac ccttttgcac cagcagggtc agggcttccg cggcccgggc ggccctcagg
    16261 acgaggaaga atctccccct gggcttcagg agaaagcgcg cggccgaaaa aacatccgcc
    16321 agggaacagg ttgtcccgtg gcgggccaca gccttggcgg aacgaggact gacccggctt
    16381 ctcccggggt cgttataggg ggggttcatg agcacgacgt caaaggattc ggccgcataa
    16441 agcttccgga tctcccgcaa gtctccccgg gaaacgtgca cccggccgtc gagcccgttg
    16501 agtcgggcat tctcacgggc aatctccaca aggtcctcgg cgatctcgag accttcgaag
    16561 gccgccgcct ccgggtactt tcgggcgagg atgaagagca cggccccgtg ggcgcagccc
    16621 agctccagga cccgatcccc ccgcttaacc ctggaagcgg cagcaagcag gatggtgtcg
    16681 acggaaaccc gggggccctc caggggctgg atgagattca gttcaccgaa gaggatatcg
    16741 tcccggcttg tcccagccct catgtccagt cctccatccg cacccccacc agctgcgaga
    16801 gccccggttc cgaaagggtg accccgtaaa gcacatccgc cttttccatc gtcgttctcc
    16861 tgtgcgtcat ggcaagaatc tgcagttccc ttgcgtattc cgcggcaagc tccacgtagc
    16921 gcctcagatt cacctcatca agggccgcat ccgcctcatc caggatcgcg atgggaaccc
    16981 gcgcctcttc catcgccgcg aacaaaaggg cgatggccgt cagcgactgt tctcctcctg
    17041 atagctgggc aagcccctgc gggcgttttc cggggggtcg ggcagtgaaa tccaccccgg
    17101 cttcccatcc ctcttgtggc ccctcaacct gcaggtgggc ttccccaccc ccgaaaagtc
    17161 tctggaacaa cgagttgaac cgcctgtcga tcccgtccat ggcgtttccg aagacccgcg
    17221 aggcctcccg gtccgtttcc gcgataaagc cctggaggga ccgtatcccc tccctgacgt
    17281 cggagctctg ctcggaaaga aacgccgtgc gctccaggag agattcctcc tcggaaagca
    17341 cccccagctc cacctcgccc atggaccgca ggaccttttc cgcgttccgg atcgaccgct
    17401 tgacctcatc gagatcgatt tccccgcacc ttccaggccc cgggtaaggg aatttctctt
    17461 cccattccgt cacaagctcg gccagcccgt tttcggcttc caggatttct cgctccagtt
    17521 cttccaggcg ggtcaggcaa cggcggctgg cttccccagc ccttccttct cgggcgacga
    17581 cccggaccag gaatttttga tccttttcca ggcggccggc aatggtacgc tccatttctt
    17641 ccaagccgct ttttgttctc cagagggcaa ggtaattctg ccccaggcgg gaaagatctt
    17701 caaaaagctt ttcgagggcg ctctcccggg aggcgattct cgcttcctcc gttttcaggg
    17761 attccctgcc cccccgaact tccttttcga cccttttcag caattccagg gttcctctct
    17821 gtttttcctc cagcaggagg acctcggttt caagtcccgt cggcccctca tccgggagga
    17881 agttctcctt ctccttctcc ttctcaaggc ttcctatctt cgcttcggcc tgccttcttt
    17941 ccaaaacgag agccaggagc gatttgccgg cctccgacaa ggttgccttc agctttctct
    18001 ccgtgtcggc cagctggcgg atgtcctttt ccgtccggtc tttccgggcg tgcagaagac
    18061 gcaacgcctc ctccgcttcc cgccgcgctg aaaaagcctg atccttttcc tccgaagcct
    18121 cgatttccat ggcttccgcg cgggcaagct ccgtttcaag gcggcggatc tccccctgca
    18181 ggttctccag caaggcctcg gtctccttcg ttctgcgacg gatttccatc ccgccggtgc
    18241 cggttttctg ggctcctccc gaaatggaac cgccgggcat gaagacctca ccgtcaaggg
    18301 tggcaaccgc aagcctgggc aggcgtctca ccagatccga ggcgaaacgg taattctcga
    18361 gaaccaggag gtctcccagc aaatgttcaa tcgccttttt ccactctttt tctgcaacaa
    18421 tcaggtccaa cgcccacccc agcactcccg gttccctggt gaggcccgga tccctggcgg
    18481 gtttaccccc ccggcaccga tcgaggggaa ggaacgtggc cctgcctgcc ccagactggc
    18541 gaagcttctc gatgcaaagc cccgcttctt ccatatccct gacaaggagc cagaactgcc
    18601 ttgctcccaa ataggactcg atggctttct cgaaacggcc cgggcattcg aacgcctcga
    18661 tgactggaac gggagaaacc gccaaccttc ccagccgcga ggcggcaatc aggaactgga
    18721 ccggcttggg aaacacctgg ccgtcgagtt tctggttcag cccttccagc tcctgggtta
    18781 cctggttttc cctttttcgg agcctggcaa gttccgtcgc cgttctctgg gaggcggcga
    18841 acgccactgc atgcctttcg acggcctgct ctgcactccg gacgagggaa agcacctggg
    18901 cctccctttc cgccccgaca gtctcgaggc gggaaaactc ctcctgcaaa ccacggcgtt
    18961 ccgcttccag ggaggcccat tgcctgccga gtgcagccag cctcgccctt tgcgtttcct
    19021 cctcggcctg cagttcggct ctcctgcggg tcagcgatct caattcctgc tcgctttttt
    19081 cccatctctg ccgccgaagc aaccggtcct cgcgaagagc aatcgcctcc gctttcttcc
    19141 tagagagctc ggcttcgagg gattgcaaaa gatcccgctg gacgcttgcc tctctttcca
    19201 ggacccgtag cgtctctttc ttggcaaggc agtccgcctg ggcagtcttg acctggtcct
    19261 tgttcatcct ggctgaggaa gcaagggctg caccttgccg aagagtttcc tgaagagagc
    19321 tggaaaccgc cgcctgtctg cggcggcaat cctccatttc cttctggagg gattcaagct
    19381 ccttttgcaa caaccccttt ctacgcatcc atcccgcggt ccagaaagaa tgcctggcct
    19441 gttcccggat caggacttcc cgacgggttt tgagcgcctc cagggcgatt tccgaccgtt
    19501 cccttcgtag ccagtaatac aaacgccgct gatcttcaag aaggtccatg acctctttgg
    19561 ctttccgggc tttctcaacc atgggagcga tttcgtcgcg gcgcgccgaa agctctgaaa
    19621 gaagggtcag caggcgcaac agttcgtctt ccgcctttct aagacggtct accgcctctt
    19681 ctcgtttcct tcggtaaaga tcgatcccga aaaggccttc gaggagcatc ctccgctgga
    19741 ggggcttttg ctggatgatt tccgaaatct cgccctgacc gataaaggcg aaccggatgc
    19801 cctccagtcc ccagtcccgc ttgagctggt ccaggtcctg gagacgggcc cgattgccat
    19861 cgatcgtcag aaggccgttg ccttccctgt cgatctttct gcggatccgg cagtcctttt
    19921 tcccggacac cagccggact gaagcctcgg cttccttgca gggggacagg ctcgcgctgc
    19981 cctggaaaag gagatcggtc tgccgcgaga ttcgcagttc cgacggggac ccctccccca
    20041 gaacccaccg aatggcatcg agaaggttgc tcttgccgct tccgttggga cctacgatgc
    20101 aggtcagccc gggcgaaagc tgcagttcgt gttctccccc gaagctttta aatcccttga
    20161 gcagcagttg agcgatatac attgaacttg tgttccgcct ggctctggga cccctggaaa
    20221 tcgaggcgga atttttccca ggggaaattc ggcatttccc gaaggaaaat cttcctttcg
    20281 aactcttcct cccagctttc caggtagttc tccacgatgt gcctggcaac cgcagggtaa
    20341 agttccagga tcagcgcttc cgccttcccg gtccgacaga ccttccgcag gaagcgcttg
    20401 atcgaaaggg cgacggtgtc ttccttcagc acccagccgt tcccagagca aaaggggcag
    20461 cttttcgtga aggttccccg aagatcactt cgggccctct tccgggtgat ttccacaagc
    20521 cccagctggg tgacgccgta gacccttgcc ttgcagcggt catggcggaa aacttcctcg
    20581 aggcgctgca aaaggatatt cctgtcctcc tcgtattcca tgtcaatgaa atccaccacg
    20641 acgattccgc cgatcgaccg gagccgcagg acccggtaaa ccgcctccgc tgcctcgagg
    20701 tttgtcttca gaaccgtttc cctcaggctc gttgccccga catatttccc cgtgttcacg
    20761 tccacgacgg tcagggcctc cgtctggtcg accacgaggg ttccgccgga gggaagccag
    20821 accttgcgct ccagcgccgc cgccagttcc ttttcaatcc cgtaaaattc aaagatcggg
    20881 acggaacctc cgtagagttc gaccctgggc ggattgaccg caaaaaaatc ccggacatag
    20941 gcagaaactt tctcgaactc ctcgggacaa tccaggacaa tttccgaaac atcgccgttg
    21001 agttcgtccc tgaggattct cccgaccagg ccgatgtccc tgtacaaaag agagggggcg
    21061 ggctgcctct cggccagtgc cgcaatttcc gcccactgct ccaggagcat cgaaaggtcc
    21121 gcctggaggg cctctcccgg aaccccctcc gccacggttc tcacgatgac tccgaatccc
    21181 tccggccgga gggcccgggc tatctggcgc aggcgcttgc gctccccttc ttccaggatt
    21241 ctcttggaaa cccccgtttc attgcctccg gggaccagaa cgaggaagcg gccgggaagg
    21301 gaaagacggg tggaaaccct cgcccctttc cccttccttg ccgttttcac cacctggaca
    21361 accacctcgg tattggcccg gatatcgacc ccgcgcgcgt cttcaaggta gagaaaagca
    21421 ttccgcccat ccccgagatt gacgaatgcg gcattcatcc ccggaaggac gctgtcgacg
    21481 cgggccttga aaatctcgcc cgctttctgc gtctcccaca tccgctcgca gaagatttcc
    21541 gcaacgcgcc catcctccag gatcgccagg cgcatttctt cagaatccag aacgtttgcg
    21601 ataatctgcc tttcgctcga catccggtca ctctccaaaa tcaaacgagg gacttaacgg
    21661 tgttcatctc ccagcctccg accgtcgtcc tcaggatcag aaggtccttc catccctcca
    21721 caaggccaga agctcggaaa gccttgacca gaaagccggg gccaaaacgg tccggctggg
    21781 cgagaacgat tctggccgaa tcgccgtcaa aatccatggc acagaggatt tcgggttcct
    21841 ggagggccga ttccacggcc agtctcaacg gtccttcccc gggaaagggg ttcctgaagt
    21901 aaacccggta ctccccagcc ttgcagaggc gactcagcgt aggcccctcc acgatagagg
    21961 cgcaaaggag agagaagccc ggtggcatcc ccctttgcca gtcttcaagc gccctttgct
    22021 cccaattccc gacccagatt tccgcgggtt ccgccaatgc cgggaccccg accggcaacg
    22081 ccggtccgag gctgattttg gggtgagggg aaaacccttc cgtcagcaga agggacaacc
    22141 ctgccctcct cgccgacctt gcgagaaggg gaggcagttc gacgtgcgga ataaatgccg
    22201 aaagcccccg tttcgcgtag agaaagcgta cccggctcac ggcccttttt cctccttcaa
    22261 ccgcgggcag tccgttgggg aaaatccgca gccctggcaa ttacccgtcc ggcaatccgg
    22321 ggtgattctc tcccgaaagg cattttcccg ttccctccag agaaagcttc gggagacccc
    22381 ggtatcgacg tggtcccatg gaaaatcctc atccgcttct cgctggcggt tggcaaaaaa
    22441 agctggatct atcccttctg aatcgaaggc ctccatccag gtgtcgaaat ggaaggtttc
    22501 cgtccacccg tcgaatcggc acccgtttct ccaggctctc tcgatcacct cggcaagtcg
    22561 cctgtccccc ctggccagaa ccgcttcgag gaacccctgg cgcgggtcat ggtaattcaa
    22621 ggagatcctg gggttttcca gctgccgttt cagccacttg caccgggcct cggtttcagg
    22681 gtaaggaagc tgggcctccc actgaaaggg cgtgtggggc ttgggaatga agccattgac
    22741 ggagaccgaa acttccgccc gtcgatgtag ttttctcagg attcgaaaag cttcttttga
    22801 gatttccaca attccctgaa ggtcggattc cgtttccgtc gggagtccga ccatgaaata
    22861 gagcttgact cgatcccacc catgttcgct catcgattcc agcgtcattc ggatattgcc
    22921 ctcgtcgatc cccttgttga tcacatctcg aagccgctgg ctccctgctt ccggggcgaa
    22981 ggtcaatccc cccttgcgga ccgttcctag ctgcgcggcc agttcaaccg aaaaggcgtc
    23041 catgcggaga ctcggcaagc taagcttgac acccttttcc gcgagttggc cggaaagagc
    23101 ttcaagcacc ggggaaagcc cggtgtagtc gcaagaagaa agagaaacca gcccaatttc
    23161 ctcccaccct gttttctcca ggaggcccat agcgatctcg acgacccttt ccgggcttct
    23221 ttcccggagc ggccggtaga tcatgccagc ctggcagaaa cggcatcccc gtgtgcaccc
    23281 gcgaaagacc tccacaggga ttcggtcatg gatgatcccc gtgttcggca caatcaggga
    23341 agaggggaca aacacggaat ccatgtctcg aacgaactgt ctcctgatcg ggaggggctg
    23401 cttcgaacga atcttcactc ccgttccgga aaattcgcag gaggccagct ggggaacgta
    23461 gacgcccggg atctcaccca ggcaggacag gcgctgctcc ctggaaagtc ctctcgtttc
    23521 ggaaatcacg cgaaggacct tcgggaacag ttcttcccca tctcccaggc agaagaaatc
    23581 gaaaaagggg gccaaaggct ccggggcgag cgcgccaggc cctcccgcca ggaccagcgg
    23641 gtcgttttcg ccccgatctt cggaacgaag aggaatccct ccgagatcca gcatggtcag
    23701 gatgttcgtc gcggtcaact cgtactggag cgtgaagccc acagcatcaa agcggttcag
    23761 cggggtttgg gactccaccg aagacagagg caaccccgag gcacgcaagg actcttccat
    23821 gtcgatccat gggcagtagg ctcgttccag gatcgtatcc tcctgggcat tcccaagagc
    23881 gtagagaatc tgaaacccca aaaagctcat tcccacctcg tacacatcgg ggaatgccag
    23941 gcacactcgg aggccggggg ttgccccacc gcttttgccc gggttcgatc cccattcctg
    24001 tccaatgtag cgcgccggct gcttgacggt tgtcaggagg gaccacaagg gggaattcat
    24061 ttcaaaaggc ctttccaaat ccttttctct ccttaaaaaa ggtcgtcgtc cggtttcggg
    24121 ttttccagaa acacgctctg ggcaagcccc agggccatcc ccagggccaa aagagaactg
    24181 cctccatagc tgaggaacgg caacggcaat ccggttaccg gtgcgatccc catgctcatc
    24241 gccattcctt cgaccgcctg gaaccagatc catccagcga tcgacgcgac aaggattttt
    24301 ccccggaggt ccctggcctt gaccccggcc gcgatgatcc tccacaaaag gaaacagaaa
    24361 agcccaagca gggcaacgcc gcccaggaac ccgaactcct ctgcaaacac gctgaaaatg
    24421 aaatccgtgt gcggttccgg aagaaaaccg agcttgctct gggttcccat catgaacccc
    24481 ttgcccagta gccgtcccga acccacggcg attctcgact ggatcacgtt gtacccggct
    24541 cccaggggat cgatgtaggg attcaggaag accatcaggc gcagcttctg gtactccttc
    24601 aggaagtgcc aggccaccgg gaaggcgaaa agcgccaacc cggcaaggcc ccaaagatac
    24661 cggaccgggg tcccggcagc gtagagagcc gtgaaaacca tgaccgacag gacgatcgcg
    24721 cttcccaggt ccggctgggc cagcacaagg atcagactga tccccgagag agccagcacg
    24781 cccgcaaggt ccttcagtcc ctcgacgggg tgcctgcaga ggtgtttcgc caggaggagc
    24841 gccagggcca ccttggtcag ctcggaaggc tgaagccgta agccggcgag accgatccag
    24901 ctttgagccc ctctcgaaac atgtcccagg atcagaacgc tgaaaagcag gaggagggcg
    24961 actccataaa gcctgtaagc catttcgagc aggcgcgcgt agccgatctt cagcaccgcg
    25021 gtgtaggcag cgaaggcaac cgccccccag atcgcctggc ggatcacgaa cacccccgca
    25081 tgtttctcca tgccgaggct agcgctgaaa atcgaaaaga ggccgacgag gaaaaggccg
    25141 gctgctgcga acagcagttt ccggtctgac aaaacgaaaa ccagccgcag gatgtcgtgc
    25201 ttttcctcct tgaaggtcat gcaggttccg ccctgcgctc cgctctcctc ttgacgctct
    25261 tgacgggaat gctcgccacc aaggccacag agcgttcctc cttcgaaaaa tcaagctcga
    25321 tcttttcttc ctcgatatcg aggtagttgc tgatgacccc gatcaaatcc ttgcggaggt
    25381 tctccatcat ctccggtgag atgtcggtcc ggtcatggat cagcaccaac tggagccttt
    25441 tttttgcagt ttccttcgat ttttccttgc cgatcacctg gaagagacgg gctagaaagt
    25501 tcatgccttt cacctcctac agtcccaggg ctttccttag aaagccgaag aaccccttct
    25561 ggtctggtgc atccaggtcc agaagcggga catcctctcc caggatacgc cgtgccacgt
    25621 tcgaaaaggc tttggcggcc agcgactcgt ccccgagcgt caggggttct cccttgttgg
    25681 tcgagatcac caccgaggtg tcgtcgggaa tgacgccgat cagctcgatg gccagaatgt
    25741 cgagaacgtc cttcacgtcg atcatgtctc cggacttgac catggccggg cgcaaccggt
    25801 tcagcacaag cctgatcggc gatttcgaca tcgactccag catcccgatg atccggtccg
    25861 catcccggac cgccgaaacc tccggggtgg tcacgacgat cgcttcatcc gctccaatcg
    25921 ccgcattccg gaatccctgc tcgattccag ccgggctgtc gatcagcgtg atatcgaaat
    25981 tctcccgcag ttcgtcgcac agtttcagca tctggtcggc gctgacagca tccttggttc
    26041 tggtttgagc ggcgggaagc aggaagaggt tctcgacccg cttatcgcgg accatcgctt
    26101 tctgaagctt gcaggcccct tccaccacat cgacaaggtt gaacacgatg cggttctcga
    26161 gccccaggac gacgtccagg ttcctcaacc cgatgtcccc gtccacggcc gccgttttgt
    26221 accccgcctt tgccagggca acggccaggt tggctgtcgt ggtcgtcttg cccactccgc
    26281 cctttccgga agtcacgacg attatccttg gatccataaa gccccctcct cccaaaaatc
    26341 gactctgctc agggcaattc cttcgcgatc agggcgtccc cctcaacggc gaagagcacg
    26401 ctcttgccgt accaggggga tgcgctgtca aacgtgctga aaaggcgtcc gattcggacc
    26461 tgtgtcgctt caaaggattt tgcgatgacc cgggaggaaa cgttcccctc cgctcccgca
    26521 tggacaagtc cctgcagcct tccccagacg acgatgttcc ctgcggaaac gacttccgct
    26581 ccgtcgttca cctgtccgat gatgaagaca tcccccgggt gttcgacttt ctgacccgaa
    26641 cgaagggatc ggtccaggag aagggtctgg acggaccgga cttgccgggg ctcggaaagg
    26701 gccgcggcag gctccccgac agacagcccc gccttgcgga gaaggctctg cccgccgatg
    26761 tcaaagctga cccaggagag aacgctcaga ccgtagttcc agatcacatc cttcagcatc
    26821 cggccgatga tctcttccgt aagaggcctt ccctgcaggt ccaggacaac ccggctgccc
    26881 tcaagaagat ggtgggcctc ctggatcgcc ttttccagcg cgggccaaac ctccggatcg
    26941 ggcaaccctt ccggaacgat gaggcgaagg tggccagccc cacctttcag aacaacggca
    27001 ggtttctctt tctccttctc catctccttc ctccctttca ccgttcaact ccgacgagat
    27061 aagccaggat ctgacccaca agaggagcgg cagccgaact cccgtgccct cccccctcca
    27121 ccagcgcgac cgccacgtat cggggcctgt ccgacggagc gtatcccacg aaccaggcat
    27181 ggtcctcacc gtgaggattc tgggcggtcc ccgtcttccc ggagagcgaa accccgaaaa
    27241 ccccggatgc acgtccggtt cccgctcgca caacctcctc cattcccttc cggacccatc
    27301 cgagggcctc gggggggagg tgaagatcga tccctgcagg atgctcctcg gccgaaaggt
    27361 gagggatgac cagtgtaccc ccgttcgcga aagcggcata aacccgggcc agctgcaagg
    27421 gagtcatgag caggaacccc tgaccgatcg aatagttgac cgtgtctccc tgaaaccagg
    27481 attccgagaa ccgtctcttt ttccactccc ttcccgccag gttgccgcgg gattctccag
    27541 gcaggtcgat ccccgtgagc tggccgaccc ccatcttccg tgaccagtca agcagtcgat
    27601 cggctcccag ccatagacca acctggtaga agtatacatc acaagaatcc ttgagagccg
    27661 tcaccagatt gacagtcccg tgtcctccct tcttccagca gcggaaaacc cggttgccta
    27721 gcttgaactg gcccgggcaa tagaccgttg tccccggggt cacgaccttc tccaccaggg
    27781 ctgccagtgc gggcacgggc ttgaaggtgg atcccggcgg gtaggcgccg ctgatcgccc
    27841 tgttcatcat cggcctgtcg ggatctttga gtagcttcgc ccactcagag gaggaaacac
    27901 cccaggtcag gggattcggg tcatacgaag gggccgagta gagaaccttg atcgctccgt
    27961 cccggacatc catggcgagg atcacgcctt tccgttctcc aaggagctgc tgcgcctcgc
    28021 gttgtgcccc aagatccaga gtcagcctca ggtcctttcc cttgactggc ggggtcatgc
    28081 tcagtcgttt tctcctcctg ccaagggcat cgacctcgac cgcctcttcc gcggcaatcc
    28141 cccggagcgt cgcttcatgg accagttcga tccccgtctt tccgatcagg tctcccgccc
    28201 ggtagttttc ctcggcacgg gaatccattt cttcccgggt gacctctccg acatagccga
    28261 caacatgggc ggccagcggc ccggcgggat aaaccctgcg gaaaaccggg gtcgggaaga
    28321 ggaacttggg gaaaaacggg tctgaaacca gggtcgccac ctggcccagt gtcaggttcg
    28381 aggctaccgt cactggacgg tacggagccc aatattgctt ttggatcgtt tcctccagtc
    28441 cttgagccgt cgcgggaatt ccctgcctct gcaggagagc cgcaacttct tccagggccc
    28501 cggcctttcg aagatccggg ggatacccga cgatatcgaa agtccgcacg cttaccgcaa
    28561 gcggagcccc atgggcgtcg gtgatctccc ccctggggct cgaagtccga atcacgcgga
    28621 tcttgttctg agtcgagagc gccgcatagg atcctccccg aaggacctgg aaaaaaaaga
    28681 gagagaccgc aagggcgaga aaggaataca ccatgaaaac ccgccagtgg ttcaagcgca
    28741 ggttcagctg tgtgcggcgg ccgatattcc tattcatgat gggatctgca gtgccggata
    28801 taaagggccg tcagccctaa aagacaggcg atcgccatcc cctgctgcgt ggccagcgag
    28861 gaggacagcc ccaggccctt gctcgtgaag attgccggga ataccgaggt cagtacgtgg
    28921 gcggcagagg accagaagaa gaatagaacc gggtttgccc gcctgccggg gggaaggctc
    28981 gaccaaaccc aggccgtcac ggccacggtc agggcatagg aggccgaagt catgccggga
    29041 acgcccgtcc acctcagatc ccaaaggagc ccggccccaa aggcgtggag gaagaggggt
    29101 tcgggttcct cgcaccggac aacggacagc cagatgagcg aaaagagcgt caggtcgggc
    29161 accagggctg taccggaaaa agcgacctgc aggagatccc tggccaagag ggagatccac
    29221 agaatgccca tcacgaccgt ccccccgtca tgaccttgac cttgtaaagc cgcgaaaggt
    29281 ctgccttcag ggtaatcctg taaagccggt aacccccgat ctcttctccg gctcccgcga
    29341 tcctgccgat cggaatccct ggcggtagga gttctgaaac gagagcagtg ctgacggaca
    29401 tgccgggctt caagtccttc tcgacaggga tgtacagaag caagaccagc ccgtttccgt
    29461 caccgttcag cacccccagg tccctcgttt catccacgac caccgggata tacagggacg
    29521 aagaggtgac cagttccgcc cacgagaagt ttgactcgac ccttgaaatt ttccctacca
    29581 acgcaccgtc ctgaacgacc gccatgccgg ggcggatccc ttccctggaa cccttgtcga
    29641 tacggatttc ggaccaccag gaggttggag gccgaagcgt cacgtgccct tctccggctc
    29701 cagttcccaa tccgtaattg acggttgtct gcagcaatcc tcgaagggcc aggttttcct
    29761 gttcaagccg gtcgatcctt tctcttagcg atctttcccc cgccacaagg tccacggcct
    29821 tgaccaccag gtccctcacc atccgggagg gccattcggg caccgccatg accctttcga
    29881 ccagatccat cgccttttcg gcgggcagcg aacccggcga aaccgctgca aggaacaggc
    29941 tgagggaaag cgtccctagg aacacgatcc agcgcttcgt cctggtacgc cccatcgcca
    30001 gcccccatcc cctagaaaga accccgctcg accgtgttca ggatcggtcg cttggaatcc
    30061 aggtcttcca gaattctggc caaacctttc gtcacagcga gcagcggttc ttcggcaacg
    30121 aaaacgggca cgttcagctc cttcgacaac cgctggctca accctctgag cagcgcgcct
    30181 cctcctgcca ggaccagccc ctgttcgtaa atgtccctga ccagttccgg gggggtgctt
    30241 tcgagcgttt ccttgatcgt ttcgctgatc ctctggatga tcggttcgat cgcttcgcgg
    30301 acctgaatgg aggagagcag gatggaactg ggcaacccgt ccgtcaaggc ccttccctta
    30361 acctccatct gcagctcttc ctcgaggggg agcacgcttc caagcgccca tttgatctgc
    30421 tccgccgtcc cctcgccgat cgcaagcttg tgattcaggc ggagcatgga aacgatcgcg
    30481 ctgtcaatct cgtctcccgc tccccggatc gatttgcaga ccacgatgcc gcccatggaa
    30541 agaaccgcaa cctcgcaggt tccccccccg atgtcgacca ccatgttccc cctgggttcg
    30601 tgaatgggga gagacgctcc caatgccgcg gccagtggct cctcgacaac ataggcttcc
    30661 ctggcacccg ccgatagggt ggcatccacg acggccctac gctccacttc ggtcacgcag
    30721 gccggtacgg agacaaccac cctcggccgg gaaaagaatc ctctcctttc gtcggccgcc
    30781 cggacaaaat gagaaatcat cgcctccgtc atgtcgaagt cggaaatgac cccctccttc
    30841 agcggcttca ccgtttcgat tcccgagggc gtcttgccga ccatggcctt cgcctcccgg
    30901 cccacggcca ggatttcctt tcgccctccc ctctgtgtcc gcctgacggc gatcaccgag
    30961 ggctcgttga tgacgatccc ctttcccttg acgtagacaa ccacgttgac cgtcccaagg
    31021 tcgatcccca catcactgcc gaaaaaaccc gaaaatagac gaaacaaggc ttccccccgc
    31081 tttcatcgtt cagtgcaccg tcacgggacg ggagctcccc cccgcgacga caaaatggcc
    31141 caccagttca attcccagca gtttgagctg ctttgaaacc gctatcgtca aggaccgatc
    31201 ctcttcgctc ggccttggat tcccatccgg gtggttatgg atcaggacga gtcctgcggc
    31261 tccgatccga accgccctcc ggcaaaggta cggagcgtca agaaacgccc cgtcgagccc
    31321 cccgaaggag agcttctccg atccaaggac cctcccctcg cggtccgtaa aaagggagac
    31381 gatccattcc cgttcctccg aaaccatcgt ctgttctagt tttcgaaggg aatcctgcca
    31441 ccggcccctt cccccattct gcccgttgca gcagaacctt cgtccaagtt cgaccgccgc
    31501 cacaagggcg gccgcctttg ctgcacccag tccagggaaa gaacacaatt ccgccggcgt
    31561 ggccctggcc agggcccgct catcgggata ctcccgaagg atccttgcag cgagggacat
    31621 gacgtcggat cctttttgac cggttctcag cagaaccgca agaagctcct ggagggacaa
    31681 cgcctccggg cccagttgca aaagtctttc tctcggccgc tcttttgcgg gaagacttgc
    31741 aaggctcatg gccaataagg gttttccgcc ctctcgcggc ccagggtcgt ctccgggcca
    31801 tgcccgggaa gcaccggaag cgcgtctgcc aggtcattca gcttccgcag agaggctttc
    31861 aaatcctttt cgcttccccc cggcaggtcc gtccgaccca cgctcctggc aaagagcgta
    31921 tccccggaaa ccagaaccgt ttcttcccct tcggaaacga ggaaacagga actgcccgcc
    31981 gtgtgtcccg gggtatggat gacccggacc gtcattttcc ccgcagggag gatttctccg
    32041 tcttccaggc tcttttccgc ttccgaacac tggatcgggg cacccagcca gcgggaaagg
    32101 ttttcctcgg cggcagccag catcgacgca tccagacggt ggatggccac tcccctggcg
    32161 gattttcccc tggccgcatc caggccagca atgtgatcgc catgtccatg ggtcagaagg
    32221 acccattcga tccggagccc gttcctttcc gcaaaatcaa gcgcctccga gggatctccg
    32281 ccgggatcaa cgagaatggc cgtcccctcg cggtcccaga tgagatacgc gttcgtccac
    32341 agagccccca tagggaaacg tcgatattcc atcttcagaa aacacccctg ctgtcgagaa
    32401 tcagagtgac gggcccgtca ttgcaaaggg aaacctgcat catggcctgg aagactcccg
    32461 tcgcaaccgg aacgccctgc tcccgcaagg cagctcctaa cgcttcgtaa agcctttctg
    32521 cctcatccgg cggagccgcc agggtgaatg acgggcgtct tcccttcctc gcgtccccgt
    32581 aaagcgtaaa ctgggaaacg agtaaaattt ctcctcccac gtcccggacg gatcgattca
    32641 tcttcccctg atcatcctcg aaaatcctca agttcaccag cttttcggcc atccaggcga
    32701 cgtctttctc cccgtccccg tgcgtcaccc ccaaaaggac acacagcccg gaaccgatct
    32761 ttcccttcac ttggccatca accttgacct ccgaccagga cacccgctga acgaccgaac
    32821 gcaaatcgat tcacccccga aacactccga taataccatt cactgtatta agctttgcca
    32881 taatccggta gaggtgttcc acgtcccgga cccggacttc aattgtcatt cttgcccggt
    32941 tcgccgcgac aacgcttgct ttcaccccga tgatgtttcc atccatggag gagatcgcct
    33001 gggaaacatc cgcgaaaagc ccagagcggt ccatcccctc gagcttcaga cgggccgtgt
    33061 agcggtcgga aatgagcctc ccccattcaa cccggacggc tctttccgac ttggcttctt
    33121 cgatattgcg gcaatccgac cggtggattg tgatcccccg ggttttagtg acatagccga
    33181 cgatcgcatc ccctggaacc gggcagcagc agttcgccag tgaaacgagc accccgcccg
    33241 ctccttccac cacgatttcc gtgtcaaatt ccttcctcgc catggccgag gtcgcagcag
    33301 cctgggaagt ttccagagac acttcctgct ggggcgccac acgggtctcc agcagcttca
    33361 tcgcaacggt agaagcgctc tggctgttgc ctccgaccgc tgcgatcaga tcttccccgc
    33421 tggagtagcc cagatcgtgg gcaacccggt tcaaggcacc ggccattgcc tgcgcgttcc
    33481 actccaatcc gggttccctc cggcggagtt ccctttcaag aagctcgcgc cccttgatga
    33541 cccgttcctc ccgctccgtc ctctcgatct gcctgaaata cgcgcggatt ttggatcggg
    33601 ccttgctgct ccgggcaatc ttgagccagt cccgcgaggg cttcccctgg ggagaggtca
    33661 gaatcctgac gatctctccg ttctggatct cgtagtcgat cgggacgatt tttccgttca
    33721 ccatcgcccc aacatactta ttgcccactt cggtgtggat catgtaggca aaatcaagcg
    33781 gagtcgaccc cgccggcaac gagacgacgt ctccgttggg ggtgaaggca aagacttccg
    33841 aggtcagcac atcccccttc aggttctcca tgaactccgt gggggtttcc ccctgtccct
    33901 ccagcgcctg tcggacccag gccaggcgct tgtccatctc gttcaggcgc gtcccgggtt
    33961 ccttgtagtg ccagtgagcc gcaatgccgt actccgcgag ccagtgcatc tctctcgtcc
    34021 ggatctggat ttcgaggggc tctccgctag gaccgataac ggtcgtatgg agggattggt
    34081 acatgttgct cttcgggttt gcaatgtagt cgtcaaactg gccgggaatc ggtttccaga
    34141 tcgtgtggac gatccccagg accgaatagc attccgcaac cgtaccgacg atcaccctca
    34201 gggcgagaag atcgtagagc tgttcgaggg aaaggttctt ccgctgcatc ttttcgtaga
    34261 tactgaaaaa atgcttggcc cggcccgtga tggaaacctc gatcccgatc tccttcattt
    34321 tctggttgag gatatcgatc gcctgcttga tgatctgctc cctttccgga agctttttcc
    34381 tgacccgccg ccggatctcg tagaacatct ccggatctag gactttgaag gaaagatcct
    34441 ccaggtcccg cttgatctgg tagatcccca gccgatgggc cagcggggcg tagatttcca
    34501 ccgtttcctt ggcgatgcgg acctgcttgt ccctccgaag ggtctggatc gtcctcatgt
    34561 tgtgcaggcg gtcagccagc ttgatgagaa ccacccggat atccttggcc atgacgagga
    34621 acatcttcct gagattctgg gcctggtaat cctcgaagga attgaaggga agcttgccga
    34681 gcttcgtcac gccgtccacc atgaggagga cgttttcgcc aaaggcgctt cgaatcgtat
    34741 ccccgctgag gtccgtgtcc tccaggacat cgtgcagcag ggccgccacg agtgtgtcga
    34801 catccagctc catgtcggcc aggatcgagg caaccgagat cgtgtggaca atgtagggct
    34861 cgcccgagga tcgcttctgc tgttcgtggg cttttgaagc aagaaccatg gcctcgccca
    34921 accgagtcag ttcgtccttg gaaaggtacc ttgaagcctt cccccaaagc tcctgccagg
    34981 caagcttgac cgaggccacc ctctgggatt cctcgatcct tccaaggaaa ctgtccctca
    35041 ggaggcgcag ggctttgctt ccctcctgcg gggccttttc ttcaggactg cctgcggatc
    35101 ctctctccgg ccccttcaag aaaggcaccc cctacccttt ctccctgcga acgaccggct
    35161 tttccaggaa aaggtccacg cgggctcgcc ctctccagaa cccttttctg gcccgataca
    35221 cccatccttc cacggagccg gcatccttca gcagttccgg cgaccggaaa gcgacaatgg
    35281 aggcttttga gaattcaatc cgggcatgca gcccatcttt gccatgggaa acgatccggt
    35341 tctcggcccc ccgctggaca taaaacaggg gcccgggatt cccgaagcca aacggtccga
    35401 gccgatcgat ctcctccagt gcttccaggg agagatcctc cgggtggaga tccaggacat
    35461 caagacagtc ggaatcgcac gtccgaagtc ctgcaagaag gattttcagg gaatccctta
    35521 ctgaaggcca atcctggcgg gccacgctaa accccgcagc ctgccggtgt ccgccccact
    35581 ccttcaggga aggggccagt ctttccagca ccgaaacggc atccgcccct ttcgggactc
    35641 tcagggtacc gcgcaccagg cctgcattca cgggcgctgc aagaacgaca gggcagccca
    35701 tttcgctgca gatccggctg gcgacaccgc tcaggacccc aacgggccaa tcgtcatgtt
    35761 ccgcgaccag gccagctttc ccttcaaaaa ggggtctggc ctcttccatg attctggcgg
    35821 tcaacccctg tctcttgcgg ttgagcacga caagatcacc gactgctttc tccagaggag
    35881 attccccgga cagcaccttg actgccagat cggccgtatc cagtcgtcca ggggcattga
    35941 ggcagggaat gaccttcatg gccagggatt cacagtcgag cgcgccgaga tccagcccca
    36001 gctggttcga aagcgcttca agtccaggtt ccttcccctc ccggatcttt tgcaaccctt
    36061 ccctgacaag ggcacggttc agcggcccca ggggaacgca atccgccacg gtcgcgagag
    36121 cgaccagccc caggttttcc agcagccagt tcctttcgaa cgctttgctt ttccaggccc
    36181 aggcccaaag cacggcagtg ccacagaggg ccttggcctc cgggttcccg gcgaactgcg
    36241 ggttgaccat aaccgcgcct gtctctgcct gacgcttttc cctgggaagg tggtgatcga
    36301 aaaccaccgc ctccatgccc agatctcttg ccgctgccag ggcattttcg cttgaagtcc
    36361 cgcaatccac ggcaacgagg acatcgcacc cccaatcggc gatttttcgc acaatccgct
    36421 catgcaggcc atagccttcc tggtgccgat ggggaataaa atagcgaacg tcaacatcgc
    36481 gaaacgaaaa aagccgcgcc gcgagggtgg tcgccgatac cccgtccaca tcgtagtcgc
    36541 cgtagacgac aagacgggta ccggctttca tccgatcgaa aacctccttc gcggcctcga
    36601 cacctggccc caggttcacc ctctccatca ggacgtccaa atccgggtga aagagatccc
    36661 tggggtcctc ttcgatcggg actccccgca tctgcaggat tgcgcccgcc agtggagagc
    36721 agccgatctc cctcgagaga tcggctgtcg tccgatcgat cctccaatgc cggagaaggc
    36781 cgggttctat ccctgcaatc actgtgggct cccgctcgct gatccttccg gatccggatt
    36841 cgcctcaacc gcccgagcca cctcggcagt ggaccccttc agggcttcga gcagttgttg
    36901 cctttcctgc tcaagagagc ggatccgctc ttcctgttcc ttcattttcc gtcggagggc
    36961 gcttcgaacc tcgatgagag aggctaggga aacgatccac atgagaagtc cccctgcggc
    37021 aaatagcaaa atatcccata gcccctgggg gagggtcttc tcccagaaga tgaaccggac
    37081 cacgacatct cccgtgttct gaaaagcata gcctgcagcc accagcatgg ccagcaggat
    37141 cgcgagggca taactcttca cattgttccc tccttcgttg cggctccaga accgcttaag
    37201 ctttcctgcc gagaggccct ccgcccgatt cgagcgtgaa cttcgggctt gtcctcattc
    37261 tgcaggcttt gcaggtacct gtccctgccg gagttctccc agatgggatc ataaatcgcc
    37321 ttcatctctt ccgcatagcg gtctaggtct cccgccatct cctgcaggat ggattccgcc
    37381 cccgggtgag tgggatgggc atgggtcctc tcgacgattt cccgaagcac atggggatgg
    37441 tcgatgatca ggcaggggcg aagaaggttg tcgtcatcgt agggctgcgc ttcgcggatc
    37501 gcctggaaaa atgaactctg gaacacttcc gttagttttt tcccatgaat gttgtcgacc
    37561 gcgaagtgga caaacacgca aggctccaca tctccgttcg cgatgatgtg aacgtagcgt
    37621 tcccctcccg ccatgcaccc gtcaacgtag gggccgtcgt tccagaagtc acccatgaaa
    37681 atcggttttt cccgcctcca ttgctcgacc ttttcgtgga ggcgcgctct ttgttccggt
    37741 gtcgccatca gggagggatc cgggttcagg ccggtcggga tgtactggaa aaaccatccc
    37801 gccaggcacc cggccgaaat catgctgtca atgaattctt ccgaagcgat ttcctccgcg
    37861 ttttcccgcg tgtaggtcgc gctgaaaccg aaaagaaccc cagccttttt cagccgggcg
    37921 taggtttcca tgatcctgtc gtgcattcct ggacctcgcc gggcatccgt gcttttcggg
    37981 cttccctcaa tcgaaaccat gggcgccacg ttgcccagcc tgaccagtct ttcgaccatc
    38041 ggctcatcca gaagggtgcc gttggtatag acctggaaaa agcagtcccc gtgcttttcc
    38101 cacaaatccg gcaggtcttt ccggatgaag cattctccgc cggaaaaggt gaagaagtac
    38161 atccccagtt gtttcgcttc cgtcaggatc cggtccagct cttcgaatga gagttcttcc
    38221 cctcctctgt actcggaagc ataacacccc ctgcagtgga ggttgcagcg catcgtcggg
    38281 ctgatgacta ggaaattggg aatgtggatc ccgcgcgcct tttcttcccg gtcccggatg
    38341 cctcttccca ggacgatgaa attgaccagg aaattcgcga tgaatttttt tctgacattg
    38401 gggtgaagct cgctgaaggt ccgcttgaaa accctgataa acggatgatc ggtttccacc
    38461 atgaaagcca atttttcaaa ccccgtcctg tggtatttcg acggggcaag cgcagagatg
    38521 atccggaaaa gggcggcaag gttcttttct cttccatcct caatagcctg gatcaggcca
    38581 tccagcagcc tggcggaaac gatccccctg gcctggtcga aaatggactg cctcaccatg
    38641 atcacgctcc tttcgcttcc agtcgttcaa tcctctcttc caggatccgg aatcgaagtt
    38701 caaaggcctg gagatcttcc ttcgtcgcta gaggcagatg cgcgagcaaa cgctggagtt
    38761 ctttctggaa accaaagatc aacgttttcc tccttgcctc cagggaccgc tcgacttcct
    38821 gaaggagccc gtccaggtcg gcctggctaa tgatcccctt ctggaccagg gaagacatgt
    38881 tgtctctcat gatcgccagc gcggccgtcc ccgttccgac tccttccatg agaagctgaa
    38941 ggaccgtttc catccgtctt cccccctttc cgttttccgc tccattataa acctcgcctt
    39001 aaaacgagag ggacggcgtt tccgcgccgt ccccccgttg tggccgatgc ctcccgccgg
    39061 ctagcgggga gagcgctgca tccactcgca aagaagagcg caggcgatga agatcgaact
    39121 ataggtcccg acgataagcc ccaccaggag ggcgaaagaa aagtcggcaa tgacgggccc
    39181 cccgaaaagg aagagcgcga tgaccgggag gagggtcgtc agggacgtgt tgatcgtccg
    39241 ggatagcgtc tggttcacgg aatcgttgag caggcgctcg atcccgagct ggcgcagcct
    39301 cccccagttt tccctgacgc ggtcgagaac cacgatcgtg tcgttgatgg aataccccac
    39361 gaccgtgagg atcgccgcaa tgaaggtcag tgaaatttct ttacccgtga gggcaaacgc
    39421 tccagtgacg atgatcgcgt cgtgagccag ggcaatgacg ctgaccgcag cgaagcggaa
    39481 ctggaaacgg aagctgacgt aaaggaggat gcccaccagg gcaagaccga gggcgatcag
    39541 ggccccgcgg ctaagctctt tcccgacgac aggtcccacc ttctcaagcc tgagaacctg
    39601 gatctcgccg aactgtttcc ggagagcttc gcgagccttc tgaccgaccg tctcctcacc
    39661 cgctttcagg cggatgatca tgccccggtc gctgtaggac tggatgacag actgttcctg
    39721 ccccacggat ttgagtgttt cccgaacctg gccgaccgag accggcgccg ggaattcaac
    39781 ctgcagaagg gttccccctg tatagtcgat ccccaggttc agaccgcgga aaaagatcag
    39841 aaaaaggctt ccgactacca ggaaaaggct gatccccatc gcaatcttgc gaaaagccat
    39901 gaaatcgaat tgcctgtcat gcaaaaactt catgctgttc cctccctcag ttcgacccga
    39961 gggccttttt tcggctgaaa tccaacagaa gctgcaggat cgcccgggta accacgacgt
    40021 tgcagaaaac gctggaaacg atcccgatgc tcagggttac cgcaaaacca cggatcgggc
    40081 cgctcccgaa atagaacagg atcattgccg caatcaaggt tgtgaggttg gaatccagga
    40141 tggtcatgag cgctttccgg aaaccggcgt caagagccgc aagccgggtt ttgcctgaac
    40201 gcagctcctc cttcatccgc tcgtagatca ggatgttccc gtcgaccgcc atcccgatcg
    40261 tcaggacgat tccggcgatg cccggtagcg ttagcgtcga cctgaaggtg atcagccccg
    40321 caaagacgag aagaagggcg acgcacagcg ccaagttggc ggcgatcccc aggaatcggt
    40381 aataaaccag catgaagacc aggaccagaa cggctccgat gatccccgcc ctgaggcctg
    40441 atcggatcga atcggaaccg agggtcggtc cgacggtcct gttttcggcc acttcaaccg
    40501 gaacaggaag tgcacctgcc cgcagcatga tcgcaagccg gttggcctcg tccaccgtaa
    40561 atcggccggt gatctgggct tctcccccgc tgatccgctc ctgcaccacc ggagccgaga
    40621 tcacttctcc gtcaaggacg atggcgatct gccggccgac gttggatgcg gtggcctcat
    40681 cgaaaagcct tgttccctcg gggctgaact tgagggaaac catcggccgg gaaagactgt
    40741 caaatcctgt tctggcgtct ttcagatcct tgccgaccac ataggttttc cccagaaggt
    40801 aggccctccc gtcacgatcc cttccgacgg tcgttcccgg cgtccgcgca gcggtcgcct
    40861 tcagttcgtt cgcaagggca tcgacttctt ccctggcctt ctgccaccgc gcctgggcag
    40921 ccaggaaatc ttcctcgctg gcgtaattgg gacggttggg cgcgggaggc acggcacccg
    40981 tggactgcaa aacctgcttg aactcgagca gcgccgttct gccgatcagt tccagcgccg
    41041 cctccgggtc ctggatccct ggaagatcga ggatgacccg gtcctttccc tgcctctgga
    41101 tgacgggttc cgtgacgccg tactggtcaa cacggtttct caggacggca atcagcctct
    41161 cgatgctgtc atcggtcaag gggttttccg gggtatcctt cgccagaagg acgatgtggg
    41221 cgcccccctt gagatccagc cccagccgga ccctcccctg gatgggaaaa accgccaaca
    41281 gcgcagctac gattaccgcc gcgacgatga tcaatcgcca gcgatccctc ttcaacatgg
    41341 gacaaacctc ctcgtgaaac aacttggttc agaaaagccg agcattcccc gtctccgggc
    41401 agaaaaaaag gagcaggcac gttcacccac tccgtctgct aaagggcagc tacgcttcag
    41461 gaatcccctc aaccgaaggg gtttcttcct tcttgccggc ttcttcagtc cgcttgtact
    41521 ggatggagga cttcaagata cgggccttga ccccgtccgc gatctcaagg ataaagctgt
    41581 cctctttcgt atcacggacg atcccgaaaa acccgccggc ggtcacgacc tgatcgcctt
    41641 tccggatcga tgcgagcatc tcctggtgct ggcgctgccg cttcttctgg gggcggatga
    41701 tcaggaaata gaaaatgacc acaaacaatg caaggggaag aagcattccc atcagtccac
    41761 cttgagctcc actgttcaaa ccgaacgcct cctatcttca gtctcgtatt ttcggcatgg
    41821 acaattctaa cacgacttta ccgttcaagc tgcttgagaa agaaaaggag cttctccagt
    41881 tccgtcgcga tatccacgtg gtagacaaga acggacttcg gaacgatcac cgctgtctgg
    41941 gcgaaggaaa ggatcccccg gatcgttccc gcggccaccg ctcggtcgac acatccctgc
    42001 gccgccgcag cgggcaccgt caggatcagg atctcgacct gctgttccct gaggacatcg
    42061 gtgatctttt cgatcggata gcaggggatc cccaggatag agcggccgac cttcgcggga
    42121 tcgacatcaa aaagggcaag gaccttgaac ttgctgctct gaaaggcctt gtgccccaac
    42181 agggcttcac ccagccgccc cacgcccacc agggcgatgc gccaaagccg gggggttgaa
    42241 agaatgctct tgatatgctc atacagtttc tcgacatggt agcccacacc ccgcttgcca
    42301 atctccccga agtaggaaag atccttccgg acttgactgg ctttcaggga aagcatctcc
    42361 ccgatctcct gcgaggaaac caccctgcgg ccctcttcat gaagaagttc caggaggcgg
    42421 tagtactgga ccagcctttc tatcgtcggc tcggcaactt tcatcaacgc gatcccccga
    42481 tctcggcgcc gcttgcctga ctactgtcgg ctggcgacga cctcgatttc gaccagggcc
    42541 cctttcggca gggcggccac ttctacgaac gagcgggccg gaggctcctc gctgaagaaa
    42601 cgaccataga cctcattgac ggccgcaaac gtccccatat cggtggaaaa cacggtcgcc
    42661 ttgacaacgt gacggagcgt cagcccctga gactcaagga tggccttgac attcttcagg
    42721 acttgctccg cctgggctgc agcgtcacct tcaacgagcg agcccgtttt cgggtcgatc
    42781 gggatctggc ccgagaggaa caggaaatcc cctgcccaaa ccccctggct gtaaggaccg
    42841 atcgctgccg gcgcattctc ggttgaaatg atttttttca cttgacccac tccctctcgt
    42901 tttatttttc aggaatccag ccctttttcc caaaggccct cccctttaac gcagcgatcc
    42961 tcgagcacgc gaaacatctc attctcgcca cgcgggatcc ttgactctcc cgagaggact
    43021 tcgacaagaa gaacccttct cgggtaagcg atttcaatcc ggtcggccga gcgaacttcc
    43081 gcagatggct tgacggtttt cccgttcagt ttcaccgcgc ccatctccac catttctcgc
    43141 gcggtggccc gccgtttcac cagtcgggag agtttgagga aaaggtcaat cctcatgctt
    43201 gatccgtccg tcacaaaccc ttcgaagtat accaaaatcc attaaccgca acaaatactg
    43261 cgctcctatt ccaggaaatc ccgcagcttc cggctcctgc ttgggtgacg cagctttcga
    43321 agggccttgg cttcaatttg ccgaatcctt tcccgtgtca cgccaaaccg tttccccacc
    43381 tcttcaaggg tgtaggcgtg cccgtcctcc agcccgaatc tcaggtgcag gacttccctt
    43441 tccctgtcgg tcaggtcctc ga
//
LOCUS       DAOOVN010000007        41989 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_588_length_41989_cov_15.443030, whole genome shotgun sequence.
ACCESSION   DAOOVN010000007 DAOOVN010000000
VERSION     DAOOVN010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 41989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41989
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_588_length_41989_cov_15.443030"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..380
                     /gene="rpsJ"
                     /locus_tag="PLY83_01595"
     CDS             75..380
                     /gene="rpsJ"
                     /locus_tag="PLY83_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003672692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOV28761.1"
                     /translation="MAKKIRINLKAFDHRVLDASAVQIAETAERTGAKVSGPIPIPTE
                     INKFTILKSPHKDKDAREQYEIRTHKRLIDIVDPTQKTMEALMQLNLPSGVDIQIKL"
     gene            420..1046
                     /gene="rplC"
                     /locus_tag="PLY83_01600"
     CDS             420..1046
                     /gene="rplC"
                     /locus_tag="PLY83_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOV28762.1"
                     /translation="MSMGILGRKIGMTQIFDEEGKAVPVTVVEAGPCPVLAVRTQEKN
                     GYTAVVLAFGAKKAKRVPKPMKVVFEKAGIEPKRWIREFRIDDVTPFQPGMDVTATIF
                     EAGESVDVTGVSKGKGTAGGMKRYGWGGGPASHGQSKWHRRPGSSGAHSYPGRVFKGH
                     GNPGRMGNERVTVKNLKVAAIDAENNLILIRGAIPGARNGLVMVRKKH"
     gene            1071..1697
                     /gene="rplD"
                     /locus_tag="PLY83_01605"
     CDS             1071..1697
                     /gene="rplD"
                     /locus_tag="PLY83_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOV28763.1"
                     /translation="MPTMKLINFEGEVVGSLELSDAVFGAPIHVPAMHQVVVAHLANA
                     RQGTASAKTRGEVSGGGRKPFRQKHTGRARAGSTRSPLWLKGGVAHGPKPRDYSQKVN
                     RKVRQLAMRSALSLKATEDRMLILENGDFGTHKTKAMLAFLGKVEATRKPLMVIDETN
                     ANIYRSVANIPGAKVIHVDSLNVYDILNHEHLIMTLASAQKLQEVYGR"
     gene            1694..1990
                     /gene="rplW"
                     /locus_tag="PLY83_01610"
     CDS             1694..1990
                     /gene="rplW"
                     /locus_tag="PLY83_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOV28764.1"
                     /translation="MNLVAHDVIIRPIITEKSNRQMEHNKYAFEVHPKANKVLIRQAV
                     EEVFKVKVESVNTIKVRSKPKRLGVFLGRSRSWKKAIVTLAAGERIKFFEGASA"
     gene            2011..2838
                     /gene="rplB"
                     /locus_tag="PLY83_01615"
     CDS             2011..2838
                     /gene="rplB"
                     /locus_tag="PLY83_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOV28765.1"
                     /translation="MAMKKFKPTTPSRRTMANSDYKEITSTKPEKSLLEPLRKTAGRN
                     NTGRVTMRHRGGGNKRQYRKIDFKRDKIGIPGKVATIEYDPNRSARIALVHYADGEKR
                     YILAPSGLSVGAQIMAGPGADIKPGNALKMMDIPVGTVVHNIELEPGRGGVLVRSAGS
                     SAQLMAKEGKYAYVRMPSGELRLVLQQCMATIGQVGNEEHENVVDGKAGKTRWLGRRP
                     KVRGMVMNPVDHPMGGGEGCSKSNKHPVSPWGTPAKGYRTRKSKPSDKMIVRRRYAK"
     gene            2858..3139
                     /gene="rpsS"
                     /locus_tag="PLY83_01620"
     CDS             2858..3139
                     /gene="rpsS"
                     /locus_tag="PLY83_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOV28766.1"
                     /translation="MARSTKKGPFVELKLLHRVEMMNETSKKAVIKTWSRRSTIVPAM
                     IGHTIAVHNGKTHIPVFITENMIGHKLGEFAPTRKFGGHAGQERASGVK"
     gene            3155..3487
                     /gene="rplV"
                     /locus_tag="PLY83_01625"
     CDS             3155..3487
                     /gene="rplV"
                     /locus_tag="PLY83_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOV28767.1"
                     /translation="MEARAISKGLNISPTKLRQVLSLIRGKKADEALAILKFTPKKAA
                     RYVEKALQSALANADNNYGMDVDKLVVSEAFADRGTAMKRFRPVSMGRAHPFKRTSSH
                     LTIVVSER"
     gene            3500..4171
                     /gene="rpsC"
                     /locus_tag="PLY83_01630"
     CDS             3500..4171
                     /gene="rpsC"
                     /locus_tag="PLY83_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOV28768.1"
                     /translation="MGQKIHPVGMRLGIIRDWESKWFASGRNYAKNLHQDLELRQWII
                     DRWKAAGVSKVEIERIGNVIAFTVSTARPGVVIGKGGTEIQKVREELQAKTGLRVMIN
                     VQEIKNPDMEASILAESVASSLEMRVSFRRAMKKAIFRAMKSGAKGIKVQCGGRLGGA
                     EIARTEWYLEGQLPLSTLRADIDYGFAEAKTIYGVIGVKVWVYRGDAAYKARKTAKEG
                     AKERG"
     gene            4178..4600
                     /gene="rplP"
                     /locus_tag="PLY83_01635"
     CDS             4178..4600
                     /gene="rplP"
                     /locus_tag="PLY83_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOV28769.1"
                     /translation="MLMPSRVKYRKPFRRPLSGRAKGATQVDFGDFGLQALDCAWITA
                     RQIEAARVAISRKMKKGGKIWIRIFPDRPFTKKPLETRMGKGKGNPEFWVAPVKRGRI
                     MFEIAGVPREVAEEAFITAGHKLPIRVRFVAREGLGGE"
     gene            4604..4819
                     /gene="rpmC"
                     /locus_tag="PLY83_01640"
     CDS             4604..4819
                     /gene="rpmC"
                     /locus_tag="PLY83_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOV28770.1"
                     /translation="MKAKDFRDLTLEELQDKHRQYKEELFNLRFQSAIGQQGNHSRIS
                     EVKRTIARILTVKREKEMAMERATARR"
     gene            4826..5119
                     /gene="rpsQ"
                     /locus_tag="PLY83_01645"
     CDS             4826..5119
                     /gene="rpsQ"
                     /locus_tag="PLY83_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOV28771.1"
                     /translation="MEERTSHRKTRIGTVVSNKMQKTIVVRVDRMAKHALYGKPVLKF
                     KKYMAHDEENTCRIGDQVLIEETRPLSAHKRWVLKEVLRRAPILGTETGGEEA"
     gene            5123..5491
                     /gene="rplN"
                     /locus_tag="PLY83_01650"
     CDS             5123..5491
                     /gene="rplN"
                     /locus_tag="PLY83_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOV28772.1"
                     /translation="MIQLRTVLNVADNSGAKKIMCVHVVGGSQRFVGSVGDVIVASVK
                     EAIPNSNISKGQVVKAVIVRTSKEIRRKDGSYVRFDDNAAVIIDNNGDPKGTRIFGPV
                     ARELREKKYMRIVSLAPEVV"
     gene            5504..5830
                     /gene="rplX"
                     /locus_tag="PLY83_01655"
     CDS             5504..5830
                     /gene="rplX"
                     /locus_tag="PLY83_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOV28773.1"
                     /translation="MGKMRIKKGDRVHVISGKDAGKEGKVLRHIPSKDMIVVEGVNLA
                     TKHVRPTQKNPKGGIIKQETPFYAAKAMLVCPACGKPTRVARAFMDDGRKVRLCKKCG
                     EIIDKV"
     gene            5850..6392
                     /gene="rplE"
                     /locus_tag="PLY83_01660"
     CDS             5850..6392
                     /gene="rplE"
                     /locus_tag="PLY83_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOV28774.1"
                     /translation="MVPRLQTKYKEEVVPRLMKEFSYKNVMETPRIEKVVVNIGVGEA
                     KVDIKYMDAAINELAQITGQRPVLKRAKKSVAGFKLREGMPVACAVTLRGTKKWEFLD
                     RLMSLALPRIKDFQGVSRKGFDGRGNFNLGLKEQLIFPEIDYDKVIRVRGMNITIVTT
                     AKSDEEAYLLLKELGMPFSR"
     gene            6407..6592
                     /locus_tag="PLY83_01665"
     CDS             6407..6592
                     /locus_tag="PLY83_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOV28775.1"
                     /translation="MSRTALEVKAKREPKFKVRKVNRCPLCGRVHSVLRKFNMCRCCF
                     RKLAREGKIPGVVKSSW"
     gene            6620..7024
                     /gene="rpsH"
                     /locus_tag="PLY83_01670"
     CDS             6620..7024
                     /gene="rpsH"
                     /locus_tag="PLY83_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOV28776.1"
                     /translation="MYVTDPIADMLTRIRNANTVFQETVDIPMSKMKLEIAKILKEEG
                     YIRNFKVLNDAKKPISMLRLVLNYGPNRERVIQGLRRISKPGRRIYVNKSDLPKVMGG
                     LGVAIISTSQGMMTDATARFNGLGGEVICYVW"
     gene            7040..7582
                     /gene="rplF"
                     /locus_tag="PLY83_01675"
     CDS             7040..7582
                     /gene="rplF"
                     /locus_tag="PLY83_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOV28777.1"
                     /translation="MSRIGRKSIPLSKNVQVSVEGRHVKVKGPKGELSMDLLPCVDVS
                     LEEGQIVVTRKDDEIKSRAFHGMTRALLNNMVVGVSEGFQRQLEIVGVGYRAQMQGKT
                     LVLNLGYSHPIQVEPPAGIEIQTDGPTKILIKGADKQAVGQLAANIRGFRPPEPYKGK
                     GIRYAGEFIIRKAGKAGSKK"
     gene            7598..7966
                     /gene="rplR"
                     /locus_tag="PLY83_01680"
     CDS             7598..7966
                     /gene="rplR"
                     /locus_tag="PLY83_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOV28778.1"
                     /translation="MIRKRSRNEMRLIRHQRLRQKLSGTAETPRLSVFRSLKHIYAQV
                     IDDEKGTTLVAVSTREKAVAEQLKGTGNIEAAKFVGKLVAERALEKGIQAVVFDRGGH
                     MYHGRVKALADSAREAGLKF"
     gene            7980..8492
                     /gene="rpsE"
                     /locus_tag="PLY83_01685"
     CDS             7980..8492
                     /gene="rpsE"
                     /locus_tag="PLY83_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOV28779.1"
                     /translation="MAARMQKATENELTERVVCIKRVSKVVKGGKRFKFSVLVVAGDG
                     NGQVGVGMGKAREIPEAVRKGIEKAKKSMVPLKKVGNTVAHPIIGRFGAAEVLLKPAA
                     PGTGVIAGAVVRAIMELGGVKDVLTKVIGRTSNPINVAYATFEALRAMRTPEETLRLR
                     GKDRKPVQAE"
     gene            8504..8689
                     /gene="rpmD"
                     /locus_tag="PLY83_01690"
     CDS             8504..8689
                     /gene="rpmD"
                     /locus_tag="PLY83_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOV28780.1"
                     /translation="MAKLRITWKRSCIGRPDKQEKTIRAFGFKKLNDTVEMEDSPQIR
                     GMIEAVHHLVDWEAIEK"
     gene            8702..9148
                     /gene="rplO"
                     /locus_tag="PLY83_01695"
     CDS             8702..9148
                     /gene="rplO"
                     /locus_tag="PLY83_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOV28781.1"
                     /translation="MHLHDLSPAPGSRRKPKRLGCGVGSGTGKTAGKGHKGQKARTGG
                     GVGRGFEGGQMPIQRRVPKRGFNNARHAVTYEVVNLQSLEERFEAGAEITAVELLSAG
                     LIHGNRNGVKILATGELTKAFNVKASAFSAKAAEKIAAAGGKAEVI"
     gene            9149..10438
                     /gene="secY"
                     /locus_tag="PLY83_01700"
     CDS             9149..10438
                     /gene="secY"
                     /locus_tag="PLY83_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOV28782.1"
                     /translation="MLDSVRNAMRLPDLKRRILFTLGVLFVFRLGAHIPTPGIDGTAM
                     SRLFEQGGVLGFLDLFAGGALRRFSIFALGVGPYINASIVMQLLVVVFPALEKLQKEG
                     EEGRKKIVAYTRWSTVGFAAVQALGLSFWLRGLGIFSGTPWDLLLVVATITTGSIGVM
                     WLGEIISDHGIGNGISLLIFAGIVARIPEAIVQTWTSVSSGQMNFLVLLLALVLMVAV
                     VAGCILLQEGQRRLPVQYAKRVVGNKVYGGQSTFIPLRVNQSGVIPIIFASSVLLLPY
                     TIARFFPGSAGAFIQRTFSPNSLVYMVLYVALIIFFAYFYTAVVFNPVDVANNMKKYG
                     GFILGIRPGKPTSDYIEKVMIRITLAGAIFLSLIALLPNLMTQIMGINTFYFGGTAIL
                     IVVGVALDTVQQIEGQLLMRHYEGILKRRDRAGLFKL"
     gene            10450..11103
                     /locus_tag="PLY83_01705"
     CDS             10450..11103
                     /locus_tag="PLY83_01705"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOV28783.1"
                     /translation="MRLIFLGPPGSGKGTQAAKLIEKVQVAHISTGDILRENVKMGTA
                     LGVKAKEFMDAGKLVPDDVIIEMMRQRLQKDDCSRGFILDGFPRTVAQAEALEKMLNE
                     LDMKLDAVILFKVSDEVVVERLSGRRGCRQCGAIYHIRFNPSLRGDSCQICGGELYQR
                     DDDREEVIRKRLDVYHSQTAPLVGYYGERGLLLEVDAGRSSDEVLKTILDAPWGRSE"
     gene            11100..11873
                     /gene="map"
                     /locus_tag="PLY83_01710"
     CDS             11100..11873
                     /gene="map"
                     /locus_tag="PLY83_01710"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOV28784.1"
                     /translation="MITLKTEEQLTRMRRAGQIVADTLRLLREHVKPGVDTYTLDAMA
                     YEHVVKCGGRPAFKGYRVPGIRSAFPGTLCISVNDEVVHGIPDRTRRLEEGDIVSFDM
                     GVEFGGYYGDAACTYGVGNVSETRRRLMEHTLESLHRALAVVRDGATVGDIGYAVESY
                     IVPMGYGLVRSYAGHGIGRKLHEPPQIPNYGRPGSGVTLKKGMTLAIEPMVMTGGEEV
                     VTGKDGWTVLTADHSDAAHFEHTVLVCESGSEILTPWES"
     gene            11964..12182
                     /gene="infA"
                     /locus_tag="PLY83_01715"
     CDS             11964..12182
                     /gene="infA"
                     /locus_tag="PLY83_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOV28785.1"
                     /translation="MAKDDVIEVKGRVVEPLPNAMFRVELENGHRILAHVSGKMRMHF
                     IRILPGDQVLIQLSTYDLTRGRIVYRYK"
     gene            12250..12378
                     /gene="rpmJ"
                     /locus_tag="PLY83_01720"
     CDS             12250..12378
                     /gene="rpmJ"
                     /locus_tag="PLY83_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOV28786.1"
                     /translation="MKVKPSVKPICEHCSVIKRHGVVRVICSKNPRHKQSQGPRRP"
     gene            12383..12766
                     /gene="rpsM"
                     /locus_tag="PLY83_01725"
     CDS             12383..12766
                     /gene="rpsM"
                     /locus_tag="PLY83_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOV28787.1"
                     /translation="MARIAGVDLPREKRLEVALTYIYGIGPARAKEILAATGVNPDTR
                     VKDLTDDESKRLRSEIESNYRVEGDLRREVAMNIKRLMDIGCYRGLRHKRGLPVRGQR
                     TKTNARTRKGPKKTIAGKKKATMKK"
     gene            12789..13181
                     /gene="rpsK"
                     /locus_tag="PLY83_01730"
     CDS             12789..13181
                     /gene="rpsK"
                     /locus_tag="PLY83_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOV28788.1"
                     /translation="MAKRVQKKGKRKEKKHVSYGVAHIHSTFNNTIVNITDKQGNVLS
                     WASGGNVGFKGARKSTPFAAQIAASQAAKLAQDHGLMEIDVVVQGPGPGRESAIRSLQ
                     AAGLQVRLIKDETPIPHNGCRPPKRRRV"
     gene            13215..13847
                     /gene="rpsD"
                     /locus_tag="PLY83_01735"
     CDS             13215..13847
                     /gene="rpsD"
                     /locus_tag="PLY83_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOV28789.1"
                     /translation="MSRYVGPACRQCRAEGVKLFLKGDRCYTEKCAIVKRNKKPGQHG
                     DSKRHAKLSEYGLRLREKQKLRRYYGMHEAQFRRFYDMATKMGGQAGHNFLQLLERRL
                     DNVVYRTGFAVSRRQARQLVCHGHFEVNGKKIDIPSYLVRAGDVISVRESSRDIGYIK
                     ENAEVAGSRSVPAWLEVNGENLSARIVALPNRDQIDSPVNEQLVVEFYAR"
     gene            13870..14883
                     /locus_tag="PLY83_01740"
     CDS             13870..14883
                     /locus_tag="PLY83_01740"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOV28790.1"
                     /translation="MDPMRPEIKLEESTPRFGRIVVEPLERGYGVTLGNALRRVLLSS
                     IKGAAITAVRIEGVLHEYSTVPGLKEDVLGLMINLKHIPLRCHSPEARILHLEVEGPK
                     EITAADIQPDSEVEFVDPSSVIGTLEKGHRLVMDLYVEQGMGYATTDRPRPSYLPIDA
                     LMVDAIFSPIQRVKYEVQNARVGQRTDYDRLVLEVWTNGVVSVPEAVSEACNILKGYF
                     ALFAEEVRLPEGEIPGRTGQEEPQRPEDSHPEDKTVALADGDSILARPIKDLELSVRS
                     ENCLLRGGVHTIGDLVSKSRSELMKIRNLGKISLREIEEKLEKFSISLPGDEASDEPL
                     KED"
     gene            14887..15240
                     /gene="rplQ"
                     /locus_tag="PLY83_01745"
     CDS             14887..15240
                     /gene="rplQ"
                     /locus_tag="PLY83_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOV28791.1"
                     /translation="MRHKVDTRRLGRYTSHRLAMLSNMAASLILEEKITTTLLKAKEL
                     RRVSERLITRAKGGTLQDRRLVAARMNNKEAVKKLFDEISPRYANRPGGYTRIVKLGN
                     RAGDAAPMAVIELVQ"
     gene            15259..16071
                     /locus_tag="PLY83_01750"
     CDS             15259..16071
                     /locus_tag="PLY83_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOV28792.1"
                     /translation="MCALRGVSFTYPGAAAPAIQSVDLTIRRGEWVALIGANGSGKST
                     LAKILNALLIPTRGDCFSCGYRTDREEHHWAIRSRISMVFQNPDNQIVASVVEEDVAF
                     GPENLGVPPCRIREIVRESLCQVGLEEKRRTPTYALSGGQKQRLAIAGALAMKPECLV
                     LDEATVMLDPQGREDVLKILRDLHSKGMTLVQITHRLEEILDATRVVVLRKGQIEWEG
                     ELPELFERVDRFPEWGLEIPPLVRLTGSLKKRKLLPESCLPSVKELLENLCP"
     gene            16062..16904
                     /locus_tag="PLY83_01755"
     CDS             16062..16904
                     /locus_tag="PLY83_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOV28793.1"
                     /translation="MSIKVDSLCHVYHPGTPLETQALSGVDLIVERGKWVAVVGQTGS
                     GKSTLAQHLNALLLPSSGRVEVDGIPTTAGASELREIRRKVGLVFQYPEHQLFAETVR
                     EEMAFGPRNWEFPEEEITNSTRKVMDLLGLPHELLDRSPFELSGGQKRRLSIASVLVS
                     RPDYLVLDEPTAGLDARGRNELLCLLKGLVKEGLGVVHVTHDLELALTMADRILVLEK
                     GKAVFCGTPGSLLEELLERPIRGLCLPPVLELVSGLRSYGYSVPYTLDSEELADCLER
                     EIRS"
     gene            16901..17707
                     /locus_tag="PLY83_01760"
     CDS             16901..17707
                     /locus_tag="PLY83_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOV28794.1"
                     /translation="MKFLNHLTLGQYVPANSPVHRLDPRCKILSVIALLAGIFLMEHP
                     VAFLAWGGLLLLLSRFSRISFRVVLQGARPVVLLLVFTAAIHLFLTKGTVLWEWGILS
                     VTREGVTMALRMGLRLFFLVLFAGFLTLTTSPMELSDGLESLFHPFERFGFPAHEMAM
                     MMTIALRFIPTLFDETDRILRAQIARGADLEHGGLFRRMKAFIPVLVPLFVIVFQRAE
                     DLALAMEARCYRGGSGRTRMNPLVWKKADTFAIAAVIGIVAFFVLLDLKG"
     gene            17711..18463
                     /gene="truA"
                     /locus_tag="PLY83_01765"
     CDS             17711..18463
                     /gene="truA"
                     /locus_tag="PLY83_01765"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOV28795.1"
                     /translation="MRYALEIAYDGRPFSGWQKQPGKKTVQGVLEEVLSRLEGADVHV
                     TGAGRTDAGVHARGQVASFELSKPWETERLLKALENNLPPEIGVLRAAIASDQFNARH
                     SAIWREYAYFLWTASYCYPQVRPFVWRVRSGWDSPDLLLACKALEGEHDFALFCPQSE
                     CPPNSVRRVLRAGIVRRREWVAFRIRATGFLFHMVRNIVQDLKSIADGKLSVDAFKLS
                     LKGRGEQRSNRGMAPASGLFLWKVGYSPSPWE"
     gene            18519..19571
                     /locus_tag="PLY83_01770"
     CDS             18519..19571
                     /locus_tag="PLY83_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOV28796.1"
                     /translation="MLGKDIGIDLGTATVLIFLRGKGVILREPSVVAIDQNNGRVLAV
                     GDEAKKMLGRTPGHVVAVRPLRDGVIANYTMTEAMLRHFLNKVSTALKMGSLFRHRVM
                     VCVPSGATDVEKRAVLEAAIEVGAREAFLIEEAMAAAIGAGLEVGEPRGNMIVDIGGG
                     TTDVAVISLGGVVVAESLRIGGDEFDEAIARFIKREFNLAIGEQTAESLKIQIGTCKV
                     KPHDEEATLIIKGRDLVQGLPRQIEVTSSMVARAIEEPVNAIIAGIRRILEVTPPELS
                     ADIIDRGIWLTGGGALLRDLAERVSEETAMPCHVAESPLECVALGTGKALESLDQLKA
                     SGAILSVIRKGLLKGR"
     gene            19609..20049
                     /locus_tag="PLY83_01775"
     CDS             19609..20049
                     /locus_tag="PLY83_01775"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOV28797.1"
                     /translation="MIEPGKSEGLLFSCIQCGRCCRRNGIVALTAQEMETISAFLGLP
                     PDEGLRRYATRQEGRLVLRDGPRGECILYEWATARCRVYPVRPEQCRKYPFWPSILQS
                     REKWEEEGKRCPGIGKEKPSLSEVTPVVSEEEDRNDLPRNDLKP"
     gene            20135..21517
                     /gene="lat"
                     /locus_tag="PLY83_01780"
     CDS             20135..21517
                     /gene="lat"
                     /locus_tag="PLY83_01780"
                     /EC_number="2.6.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0045484 - L-lysine 6-transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine 6-transaminase"
                     /protein_id="HOV28798.1"
                     /translation="MSYTCGRNFNVTPDEVFPTIETTLLRDGFDIIIDMEKSKGSHIV
                     NLKDGSEWLDFYTFFASAPFGMNHPMLTTEEFKERIFRAAINKVASSDIYTMEMADFM
                     KTFNEVAKPEGFNHTFFIDYGTLAVENTFKTSMDWKVRKLLKAGKVSKGDAIDGKKGT
                     KVIHFRDAFHGRSGYTLSTTNTYDPNKYQYFAKFDWPRIIPPKITWPLEQNLANVEWL
                     EEVAVSQIKKAIWSNPDDICALIIETIQGEGGDNQFRTEFFKQLRQICDENEIILIFD
                     EVQCGMGITGKMWAWQHHAPVKPDIFSFGKKAQICGFVAGPRFDELEENCFTVSSRIN
                     STWGGNVVDMVRAQRYLEIYREEKILDYVSNVAGPRLLNGLLEIQKEFPDMVRNTRGK
                     GLMCAFDLVTHDLRDKVLRACWEEKMLILPCSTQSIRFRPALNVPTEDLDKGLEIVRK
                     ALKKVKGCCC"
     gene            21725..22927
                     /locus_tag="PLY83_01785"
     CDS             21725..22927
                     /locus_tag="PLY83_01785"
                     /inference="COORDINATES: protein motif:HMM:NF022727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3084 domain-containing protein"
                     /protein_id="HOV28799.1"
                     /translation="MRDFLGEFNWMLILSLIVASAIVAYVGDVVGMRIGKRRISLFGL
                     RPRYTSSFITVLTGILITVVTLWLVSMTSETVRTALFSMKYVQRQITQLTSQLQESRQ
                     ELQEMEFKLFASQDELQKKQAELKEAQGKFEKVQAQLAQVRKDLKLQVERSRAVEAER
                     VRLEGELKGLENRRASLEKSVSTLKAEMESLKRGLEQVREGQIIVLAKEVLGQVPLSP
                     KSSRESVRQALRTLVEQARAELAFRSGVKPEQVQIVTEKGNELENPDPFAGKPDRQVI
                     RLVAESNAVREEPVLVSLESHPSRLIFKKGQELGRRKVPGPLKREEAERELFLLLREV
                     NAFSVKEGVIPDPLRGTVGNLSAGDFYGSVEKLVESDSPSVVIVEAEEDVFSEGPVRV
                     RIVLKKAS"
     gene            23181..23606
                     /locus_tag="PLY83_01790"
     CDS             23181..23606
                     /locus_tag="PLY83_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOV28800.1"
                     /translation="MPLEKGYLFYDSKLAVKADELQDLYRFTHWGRSRSLDQIERMLA
                     GTSICFSARHGGRLVAFCRILTDFVFRASLWDVLVHPDHQGKGLGTALIRYAIEHPAI
                     REIPLVVTYTSELAPFLAQLGFEPKEGLLMLLRCPIEYS"
     gene            23716..24921
                     /gene="metK"
                     /locus_tag="PLY83_01795"
     CDS             23716..24921
                     /gene="metK"
                     /locus_tag="PLY83_01795"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOV28801.1"
                     /translation="MKNEKSFITSESVTEGHPDKLADQISDGVLDAILKEDPMGRVAC
                     ECMVATGLVMVAGQITTECYVDIPNLARKIVKEIGYTRAKYGFDGDTCAVLTAIDEQS
                     KDIAQGVDRALEVREAHMSEEEINRIGAGDQGMMIGYASNETEEYLPLPIALAHRLSR
                     GLASLRKEKILPYLRPDGKTQVTVEYQGDKPIRVETVVVSTQHHPAVDQAEIEADLVE
                     RLILPTIPEYLRERKPRILVNPTGRFVQGGPLADTGLTGRKIIVDTYGGVVPHGGGCF
                     SGKDPTKVDRSAAYMTRYAAKNLVAAGLAEKCQIQVAYAIGVAHPVSLMVHTYGTGKL
                     PDETLTRLVSEVFDFRPAAIIRDLDLRKPQYRRLAAYGHMGRVDLNPLPAWERLDRVE
                     QLSAAAKTA"
     gene            25077..25655
                     /locus_tag="PLY83_01800"
     CDS             25077..25655
                     /locus_tag="PLY83_01800"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOV28802.1"
                     /translation="MPKRCLWCGGGFFPCGILSHTTFLRAGTWHEGAARELVHLVKYS
                     GHRRLAFEMGRALARLYREDSNVALVPVPLHRGSSRSYNQAERLARGMSEEWGVPLVE
                     GLTWKLSFPSQAGRNAFERRALPRGAFRWEGPRMERDILIVDDVCTTGMTLLRAAEAL
                     REAGIKVKGAYCWSVSPMETLNVTWKGKASVE"
     gene            complement(25932..26147)
                     /locus_tag="PLY83_01805"
     CDS             complement(25932..26147)
                     /locus_tag="PLY83_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HOV28803.1"
                     /translation="MECLATFDTTHMALLFEKACRARGISARIIPVPRELSASCGLAC
                     TYPCENEEAVEEICREKKVEVAGFHHL"
     gene            26265..>26704
                     /locus_tag="PLY83_01810"
     CDS             26265..>26704
                     /locus_tag="PLY83_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOV28804.1"
                     /translation="MVSSKKAVLLAVLSAFILTIVAGVALAEEKIGVIEPQKVLFQHP
                     KMAQVQKQVQAVVQKKQEEAKAAIDKEQDNKKKAEIYQTKRNEAATEEQKLMAPLFKD
                     IDLAIRSVAKAKGLTIVLDKAQVFMGGIDITDDVIQELKKKFSN"
     assembly_gap    26705..26804
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<26805..27490)
                     /locus_tag="PLY83_01815"
     CDS             complement(<26805..27490)
                     /locus_tag="PLY83_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV28805.1"
                     /translation="MTEAQDQKQPLLSVRDLHVSYGAIKALRGFNLDVYPGEIVCVIG
                     SNGAGKSTLMNAIMGNVKRQSGKILFEGKPLEGQSFQIVTKGLSLSPEGRKVFAPLTV
                     YENLMMGSFPVNDKEKVKKDLEWVYTLFPRLRERRSQYAGTLSGGEQQMLAIGRALMA
                     SPRLLLLDEPSLGLAPIIIKDIFKELKRINEEGVTILLVEQNARQALLLSHRGYVMQT
                     GQLVMEGDAK"
     gene            complement(27487..28266)
                     /locus_tag="PLY83_01820"
     CDS             complement(27487..28266)
                     /locus_tag="PLY83_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV28806.1"
                     /translation="MSEAVLKLDHVNKYFGGVHAVQDMSFSLEKGELAGLIGPNGAGK
                     TTIFNLITGVYDVDSGSILFCGEDITPYKTYEVIRKGIARTFQNLRLFPRSTVLENVM
                     TACQQHYQYGFFEAATHLGRWKSLEKSIRDQSMALLERVGLDSLAEQAAGTLPYGHQR
                     RLEIARALSLQPRLLLLDEPAAGMNPDEVQELNGLITSIHRDFDLTILVIEHHMELVM
                     EICPHIICMNFGAKIAEGDPDHIQNHPEILKAYLGEEVLEA"
     gene            complement(28263..29153)
                     /locus_tag="PLY83_01825"
     CDS             complement(28263..29153)
                     /locus_tag="PLY83_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOV28807.1"
                     /translation="MSGYVEGIIILLFINGIAAMGVSLLTGFTGVFTLGHAGYMAIGA
                     YSAAIMTVRYGIHWLPAILAGGIIAMVVAYLIGVPTLRLMGDYYAIASIGLGESIRLI
                     LENWQSFTRGARGYPGIDNYTNLPVAVTFFVIMALLMFFLIRSNFGRAFKACRDDYVA
                     ASLLGFNTARYRVLSLSISAFYCGVAGALMAGFLTFIQPVMFDLNKSTELTAVVVFGG
                     LGSLSGSLIGTAVITLVTELFRPISQYRMLIYGAVMVIIMVVRPEGIMGQHELSPRFI
                     RSLFNRKDRSPRTAEGGDGR"
     gene            complement(29165..30046)
                     /locus_tag="PLY83_01830"
     CDS             complement(29165..30046)
                     /locus_tag="PLY83_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOV28808.1"
                     /translation="MDTLIQQIINGLSLGSVYALIAVGYSLVYSILLFSNFAHGGFLV
                     IGGYICYYTLAAMGHNIWLSGLLAMVGAGVVAILVERLAYKPIRERTNVTLYLLIASM
                     GMSIVIENIFVVTVGGRFRALPPVIPTDPVNFFGLATTSAFDLLSLGMAVLFLTALQL
                     FLSKTKWGLAIRAASYDLRTSGLMGVNVNRLISIVFFVAGLLAAVGGIFLSVRYTLYP
                     QLGFITIKAFVAAVIGGLGSLPGAVLGSLILGLAEMLTAGFISSQMRDLVVFGMLVLT
                     LLWRPSGLLGKQVSEKV"
     gene            complement(30190..31344)
                     /locus_tag="PLY83_01835"
     CDS             complement(30190..31344)
                     /locus_tag="PLY83_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOV28809.1"
                     /translation="MRKLGLLLVALALAVVFAGSAMAANTIKVGYLAALTGDWAAYGQ
                     TELNSAKLAVDEINAKGGVLGKKLELVPYDFRTRPEDAVNAFRRMAENDKVVAVIGAN
                     GSGINIATAPLSTKYRIPQIGTVSTNLNVTVTPEGKLNPYMFRICFTDPYQGQVIAYF
                     TAKNLKKMKAAVLYDVGSDYSQGLRDFFMQYFKKYGGSVVADQGFRAGTDVDFRAQLT
                     TIRDSKAEVLVLPNMGKEMALIMKQARELGMKDIVFIGGDGYGDFMWEIAGPSMEGSY
                     WINHVDPADPAMKPFFDSYKKKYNDECKEFVNGVLAYDSVYWLADAIKRAGKVDSTAI
                     RDALEKTKDVKLHHAVMSIDPKTHNPMNKTAVVLLAKGGKAVFYTKVQPK"
     gene            31495..32382
                     /locus_tag="PLY83_01840"
     CDS             31495..32382
                     /locus_tag="PLY83_01840"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOV28810.1"
                     /translation="MKLLITDIDGTLTDGNRVPEEVAEACGRLRARGWKLMIATGRIW
                     ASARPFAEAIGSDLPSIVYDGARVLQDGGSTPLPFREWKIPNRTADRVLALGWGSSLL
                     IQAYGDEEVTCRPSDEVTQRFFRWLGVPVSPDLKDPRLDFDPYRIIFYGDPKEARALG
                     ESIREILATEVEVTLAGEGFLDILPAGVSKGAAFRAYRTTAGLRPEVVVAAGDHLNDI
                     DLLLEADLAVTFEDAPLEVRAACHLVLPPASCMGFLELCRFLERGEFPRFFSNIDRKH
                     KRGCPVFLRGQPPETKREV"
     gene            32385..32882
                     /locus_tag="PLY83_01845"
     CDS             32385..32882
                     /locus_tag="PLY83_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HOV28811.1"
                     /translation="MSAVKGWQVERMEIPEGCNIILGQSHFIKTVEDLFEALVTSSPG
                     LQFGIAFCEASEPCLIRRDGNSPDLVEAAVRNALALSAGHAFVVLIRNGYPINVLNRI
                     KEVQEVCGIFAATANPLEVLVFETEQGRGIAGVVDGFSSRGVEEESHVEARKNLLRNI
                     IGYKR"
     gene            complement(32903..34291)
                     /locus_tag="PLY83_01850"
     CDS             complement(32903..34291)
                     /locus_tag="PLY83_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOV28812.1"
                     /translation="MVAFGMGAAIILLAFVLLGTLSGRGIRSVADYALAGRSAGTLEV
                     SGILLGALVGGASTVGTVEMAYRWGLSAWWFTLGGGIGCLILGLWFARPLRRTGLTTI
                     PEFLAREYGRKTGIVALAASTLGTFVSVVAQFLAGLAMIQGIWPIPDTVAAGVVSLAV
                     LGFILLGGLKSYGVLGKAKIAFLYFVLAVCAAVTAMEGRNLPPLWNALPFHPWFNLFG
                     RGVGVDLNAGLSLLVGVMTTQIYIQAVFSASSEETARKGALLSAVLMPPLGLFAIWVG
                     LGMRIRQPGLVASQVLPKFLAQHFPPFVAGVLWSGIAITIIGTAAGLCLGIATNLVRD
                     LPSALRRKEAGEKDRLLTSRLALLALVFLSAFLALLAPGSLILSWSYLSMGLRGAGTF
                     LPFSIALLAPGRLSARWAFLSSLAGLVLTLSWPLTRLPGNPLFSGLLVSALCCLLGWK
                     RKGTACGGPSPD"
     gene            34420..34785
                     /locus_tag="PLY83_01855"
     CDS             34420..34785
                     /locus_tag="PLY83_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOV28813.1"
                     /translation="MNLRQIAACIGAEILHGEPLLEKVEIERAYASDLMSDVLAFASP
                     GTLLLTGLTNVQIIRTAQMMDIPAVVFVRGKRPQEEAVNLARSVGMPLLVSSKSMYET
                     CGLLYAAGLSPCVIPHREA"
     gene            34789..35235
                     /locus_tag="PLY83_01860"
     CDS             34789..35235
                     /locus_tag="PLY83_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HOV28814.1"
                     /translation="MDSRYEVEFPVRGDDFLAAGEASNQIKETLVMLGVPSAISRRAS
                     VITYEAEMNLVIHAGGGILRAIITPERLTIVATDLGPGIPDLALAMKEGFSTASDRVR
                     EMGFGAGMGLPNIKRNADVLEIDTEVGRGTTLTATVFFGRQDAREG"
     gene            35239..36552
                     /locus_tag="PLY83_01865"
     CDS             35239..36552
                     /locus_tag="PLY83_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOV28815.1"
                     /translation="METGVKIVEGSCKGCVNCIKSCPTEALRVIEGKVNILPELCIDC
                     GECLRTCREKALALDEDDWDLLKSHGPVTLMADPTFCAQFSHYWSPALIREALASLEM
                     QDLFDQASVAFDLAALATANQLQEASRESLPLISIYCPAVVRLIQVRFPELLHRLVKT
                     ETPLETGTLLWRRENESKSGVVLVTPCSAKVMLVRGPVGRTRSTMGHVVSVKRLARDL
                     LAAGPKVEGKSAAPVGSRWLAWALRGGESRHVRAFSERKLTTMAVSGLRNTIDLLQDL
                     ELGRLKGVDFVECRVCDLGCIGGIGNAESRFLSELRLSRQPIEWKVSAGEHAALQELY
                     ETGIWKLEQPIQPRPRPSLADTLDEAMKKLKEMQALYAELPHIDCGACGRPSCRALAE
                     DVVRGEGELTDCVFKLRERISLLGEEITLLSGKLPHAFHERKHGK"
     gene            36549..36884
                     /locus_tag="PLY83_01870"
     CDS             36549..36884
                     /locus_tag="PLY83_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine kinase"
                     /protein_id="HOV28816.1"
                     /translation="MTVSSVIEALGLTVHHEGKGNCPVSRGVVGDLLSFVMGGAPEGA
                     VWVTLQTHVNIAAVAVLKDIPLIILASGRNPDEELLERCRLENIGLAGASASPFDVCG
                     ALHDLGLKG"
     gene            36893..37645
                     /locus_tag="PLY83_01875"
     CDS             36893..37645
                     /locus_tag="PLY83_01875"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOV28817.1"
                     /translation="MRPVRADLHIHTVLSPCGGLDMGAPEIVARALKCGLEVVAVTDH
                     NTCRNAAAVMKAARGKQLVVFPGLEVQSSEDIHAVVLFGTLEEALDFEAWLWEGFPKV
                     LNRPELFGEQILIDEENRILAEEPILLVQGARRSIDEIVTEARSRNGVVFLSHVDREA
                     FSYPAVLGPIPVDYPVDALELTCYATEKTVENCRKACPSFPLIRNSDAHTLAQIEPRC
                     STAFLVEEPDFQNLRRAFRNEGGCRVLDPWKV"
     gene            37706..37909
                     /locus_tag="PLY83_01880"
     CDS             37706..37909
                     /locus_tag="PLY83_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV28818.1"
                     /translation="MLEEKPEELSAPQRKLQCWCGRRMILVESFPDDGTFRYACPLLL
                     AGDTNHDQLLSEEEYIEEPDEKP"
     gene            complement(37916..38440)
                     /locus_tag="PLY83_01885"
     CDS             complement(37916..38440)
                     /locus_tag="PLY83_01885"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOV28819.1"
                     /translation="MGRNRKKRTALTLVEILVVLLLVGILAGAMMLVSFSVTNKAEAT
                     RILEDMRSMKSAAVLYHADYGRWPVWMKRGTTIQDMNGNAGPGRYLEKIPLASDHWIG
                     VAGASTDRAEATVIFVSLSVNRDILARLQTLGGALPLYGMTVKGPLFSIPESPSSFDA
                     ATHNAALWFIRKPE"
     assembly_gap    38607..38706
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            38802..39185
                     /locus_tag="PLY83_01890"
     CDS             38802..39185
                     /locus_tag="PLY83_01890"
                     /inference="COORDINATES: protein motif:HMM:NF013422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HOV28820.1"
                     /translation="MEHRLLEDLFKARGLLPEGEIRSPEGETPRQEKPVHQPDYSIVE
                     RIVLRFERKGHGGKSVTRVEGLPFAEEVLEDLARELKKAMGCGAWVAGNEIFLQGDLT
                     ERSQAWFQKKGAKRVVISKAGGFRK"
     gene            39182..40117
                     /gene="glsA"
                     /locus_tag="PLY83_01895"
     CDS             39182..40117
                     /gene="glsA"
                     /locus_tag="PLY83_01895"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HOV28821.1"
                     /translation="MNFRAFREEDLAALVDEVRPLASEGKVATYIPELARGNPESLAV
                     AAVDGKGSEVAAGDRDLRFTMQSISKVVSLAVALSEFGEKAVFGRVGMDPTADPFNSV
                     MRLEMVRPHRPQNPLINAGAIVVDSMLLARGPEEAFHSIREMARRMTGNPQLRENEAV
                     YLSEKRTGDRNRSLAYFLKSVKTIRGDVEEILDVYFRQCSLEVTVSDLAVLAGTLAFD
                     GKNPLTGETVLPGKICRILRALMATCGLYDGSGEFAVRVGIPAKSGVGGGIVASVPGV
                     MGISVCGPALDHQGNSIAGIALLERLSDRFGLRVL"
     gene            complement(40183..40677)
                     /locus_tag="PLY83_01900"
     CDS             complement(40183..40677)
                     /locus_tag="PLY83_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOV28822.1"
                     /translation="MKKTEENLKEAFAGESMANRKYLAFARQAEAEGFHQAAKLFRAA
                     AEAETIHAIDHLKALGMIRSTEENLRAAIEGETYEFTKMYPGFVENAKQEGAEPLARK
                     LDLVGKAEAVHAHLYEKALQTLHIGETTDLFLCTVCGHIAEGDAPDTCPICGAKKQAY
                     RKID"
     gene            complement(40760..41815)
                     /locus_tag="PLY83_01905"
     CDS             complement(40760..41815)
                     /locus_tag="PLY83_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV28823.1"
                     /translation="MSDRTVKFVHGREGVDPFLGSYERKGKIVIPTDSGRSALVLALR
                     HLGGNGFSGTAWIPAYSCPSLPAVFRKSGIRLRFYENSAGFKPVFPGSGPSRGDLLLI
                     IHYFGFPNHGALAWFRSRSPENRPFLVEDCAGASLSGNVGLEGDFALFSFRKFFQVPD
                     GGALVSSFPVQADLAPPDPRIEEKREKAFGYLQKGEFEKGLSLLEEAEADLEASFSSL
                     PRRPSSASWEKLNAENFPEEAHRRRVFGKLILDHLSQPAFESCGIRPLFGSVGEDAAP
                     LVFPVRVAFDANRIRFLLRKSGYECPSLWRLNPALRFSFPSAYALGERTVGLPLPLQG
                     KDEDLELLFRSLEMASR"
BASE COUNT         9377 a        11168 c        13070 g         8174 t
ORIGIN      
        1 ccgggaaggc ggccgcacgg tgggtgccgg cgtcgtcacc gagattctcg actagccgtc
       61 gggaggggac agccttggca aaaaagatcc gcatcaacct aaaggcgttt gatcatcgcg
      121 tgcttgacgc ttctgcggtc cagatcgccg agacggcaga acgcacgggc gcgaaggttt
      181 caggaccgat tccgatcccg acggagatca acaagtttac gatcctcaag tctccgcaca
      241 aggacaagga tgctcgggag cagtatgaaa ttcggacaca caagcgcctc atcgacatcg
      301 tcgacccgac gcagaagacg atggaggcct taatgcagct gaatctccct tccggagtgg
      361 acatccagat caagttgtaa cttgcggggg gagctctgag cgaaaaggag ctgaatcgga
      421 tgagcatggg aattttggga cggaagattg gaatgacgca gatcttcgac gaagaaggga
      481 aagccgtccc cgtaacggtc gtggaagcgg gaccttgccc ggtcctcgcg gtcaggacac
      541 aggaaaagaa cgggtacacg gccgtggtac tggccttcgg agcgaagaag gcgaaaaggg
      601 tcccgaagcc aatgaaggtc gttttcgaga aggcgggaat cgagccgaaa aggtggatca
      661 gggagtttcg gatcgacgat gtcacgccgt tccagcccgg aatggatgtg acggccacga
      721 ttttcgaggc cggggagtcc gttgatgtaa ccggcgtgag caagggaaaa ggcacggcag
      781 gcggaatgaa gcgttacggc tggggaggcg ggcctgcatc ccatgggcag tccaagtggc
      841 atcgccgccc cggttcctca ggggcccaca gctacccggg gcgggtcttc aaagggcacg
      901 gaaacccggg gcgcatgggc aacgagcgag tcacagtaaa gaacctgaaa gtcgctgcga
      961 ttgacgcaga aaacaacctg atcctgatca ggggggccat tcccggggcg cgtaacggcc
     1021 tggtgatggt ccggaagaag cactaggcgc cggaaggagg gggaaagacc atgcctacca
     1081 tgaagttgat caattttgag ggcgaagtcg taggaagcct cgaactttcc gacgccgttt
     1141 tcggtgcgcc cattcacgtt cctgcgatgc accaggttgt tgtggcccat ttggccaatg
     1201 cgcggcaggg gactgccagc gccaagacac gaggagaagt gagcggcgga gggcgaaagc
     1261 ccttcaggca gaagcacacc ggacgggccc gcgccgggag cacgcgttcc ccgttgtggt
     1321 tgaaaggtgg cgtcgcccat ggaccgaaac cgcgcgatta cagccagaag gtgaaccgca
     1381 aggtgcgtca gctggcgatg cggagcgcgc tttctctgaa ggcgacagag gatcggatgc
     1441 tcattctgga gaacggggat ttcgggactc acaagaccaa ggcaatgctc gcgtttctag
     1501 ggaaggtcga agcgacccgc aagcctctga tggtgatcga cgagacgaac gcgaacatct
     1561 accgctccgt ggcgaatatt cccggcgcga aagtgatcca cgtggacagc ctgaatgtct
     1621 atgacatcct gaaccacgaa cacctgatca tgacgcttgc gtccgcccag aaactgcagg
     1681 aggtgtacgg ccgatgaatc tcgttgcgca tgatgtgata atccggccga tcatcacgga
     1741 gaaatcgaac cgccagatgg agcacaacaa gtacgccttc gaagtccatc cgaaggccaa
     1801 caaggtgctg attcgccagg cggtcgagga agtcttcaag gtgaaggtcg aaagcgtcaa
     1861 cacgatcaag gttcgctcca agccgaaacg gttgggagtg ttcctggggc ggtcccgctc
     1921 gtggaagaaa gccatcgtga cccttgccgc gggagagcgc atcaagttct ttgaaggcgc
     1981 gagtgcgtag ggaacgagag gagaacgaac atggcgatga aaaagttcaa acccacaacg
     2041 ccgagccgcc ggacgatggc gaactcggat tacaaggaaa tcacctcgac aaagcccgaa
     2101 aaaagccttc ttgaacctct ccggaagaca gcgggccgaa acaacaccgg tcgggtgacg
     2161 atgcgtcatc gtggcggggg aaataagcgc cagtaccgca agatcgattt taagcgcgac
     2221 aagatcggaa tccccggcaa agtggcgacg atcgagtacg atccaaaccg ttcggcgagg
     2281 atcgcgctgg tccactacgc ggacggagag aaacgctaca tcctcgcccc ttcaggcctt
     2341 tccgtgggag cgcagatcat ggcgggcccc ggggccgaca tcaaacccgg caacgcgcta
     2401 aaaatgatgg atatcccggt gggcaccgtt gtccacaata tcgagctgga acccggccgc
     2461 ggcggcgttc tggtccggtc tgccggatcc agcgcacagc tgatggcaaa agagggcaag
     2521 tatgcgtacg tcaggatgcc cagcggggaa ctgcgcctcg ttcttcagca gtgcatggcc
     2581 acgatcggcc aggtggggaa cgaggaacac gagaacgttg tggatgggaa agctggaaaa
     2641 actcgctggc ttggccgcag gccgaaggtc cgcggaatgg tcatgaatcc cgtcgatcat
     2701 ccgatgggcg gcggcgaagg gtgcagcaag tcgaacaagc atcctgtttc tccatggggt
     2761 actccagcca agggatacag aacccgcaaa tcgaagccgt ccgacaagat gatcgttcgg
     2821 cgtcggtacg cgaagtagag gctgaggagg gaaggacatg gcaaggtcca cgaagaaggg
     2881 cccctttgtc gagctgaagc tcctgcaccg ggtcgaaatg atgaacgaaa cgtccaaaaa
     2941 ggcggtcatc aagacctggt cgaggcgctc gacgatcgtg cccgccatga ttgggcacac
     3001 catcgcggtt cacaacggga aaacgcacat tcccgtgttc attacggaaa acatgatcgg
     3061 gcacaagctc ggcgagtttg cacccacgcg caagttcggg ggacatgccg ggcaggaacg
     3121 cgcttccggc gtgaagtagg gggttgagga agagatggaa gccagagcga tctcaaaggg
     3181 tctgaacatc tccccgacga agctgcgcca ggtactcagc ctgattcgcg ggaaaaaggc
     3241 ggacgaagcc ctcgcaatcc tgaagttcac ccccaaaaag gcggcgcggt acgtggaaaa
     3301 ggcattgcag agcgcgttgg ccaatgcgga caacaattac ggaatggatg ttgacaagct
     3361 tgtcgtttcc gaagcctttg ccgaccgggg aactgccatg aagcggtttc gccccgtctc
     3421 gatgggccgg gctcatccgt tcaagcggac gtccagccac ttgaccatcg tggtctcgga
     3481 acgctaagga ggggtgaccg tgggtcagaa aattcatccc gtggggatgc ggttgggcat
     3541 cattcgcgac tgggaatcca agtggttcgc cagcggaagg aactatgcga aaaaccttca
     3601 ccaggatctc gagctacggc agtggatcat cgaccgctgg aaggctgcgg gggtttcgaa
     3661 ggtcgaaatc gagcgaattg gtaacgtcat cgcgtttacg gtttcgaccg cccggcctgg
     3721 ggtagtaatc gggaaaggtg gcaccgaaat ccagaaagtc cgtgaagagc ttcaggccaa
     3781 gacagggctt cgggtcatga tcaacgtcca ggagatcaaa aacccggata tggaagcttc
     3841 gatcctggca gagagcgttg cctcctcgct ggaaatgcgg gtcagcttcc gccgcgccat
     3901 gaaaaaggcg attttccgcg ccatgaaatc cggcgcgaaa gggatcaagg tccagtgcgg
     3961 aggacggctg ggcggagccg aaatcgcaag aacggaatgg tatctcgagg gccagctccc
     4021 tctttcgacc ctgagagccg atatcgatta cggctttgcc gaagccaaga cgatttacgg
     4081 agtcatcggc gtcaaggttt gggtttaccg tggcgatgcc gcctacaagg cccgaaagac
     4141 ggccaaagag ggcgcaaagg aaaggggtta atgcaccatg cttatgccta gccgcgttaa
     4201 atacaggaaa cccttccgaa ggcccctgag tggtcgagcg aagggagcca cccaggtgga
     4261 tttcggggat ttcggcttgc aggcgttgga ttgtgcctgg atcaccgcaa ggcaaatcga
     4321 ggcggcccga gtcgcgatct ccagaaaaat gaaaaagggc ggcaagatct ggatccggat
     4381 tttcccggac cgtcccttta cgaagaaacc gcttgaaacc cgtatgggaa agggaaaggg
     4441 aaacccggaa ttctgggttg ccccggttaa gagagggcgg atcatgtttg agatcgccgg
     4501 tgtccctcgg gaagttgccg aggaagcctt catcacggcg ggtcacaaac tgccgatcag
     4561 ggtccggttt gttgcccggg aagggctggg tggtgaatag accatgaagg caaaggattt
     4621 cagggatctc acgctagaag agcttcagga caagcatcgt cagtacaagg aagaactttt
     4681 caacctgcgt tttcagagcg ccatcgggca gcagggaaac catagtcgaa tttcggaagt
     4741 caagcggacc atcgcacgaa ttctcacggt gaaacgcgag aaggaaatgg ccatggaacg
     4801 cgcgacggca aggaggtagc ggacgatgga ggaaagaacc tctcatcgga agacgcggat
     4861 tggcaccgtc gtcagcaaca aaatgcaaaa gacgatcgtg gtccgggtgg atcggatggc
     4921 aaagcatgcg ctctatggca agccggtgct gaaattcaag aagtatatgg cacacgatga
     4981 agaaaatacc tgccggattg gcgaccaggt gctgatcgaa gaaacgcgtc ccctgagcgc
     5041 ccataagcgg tgggtgctga aggaagtcct gcgccgtgcc ccgattctgg gtacggaaac
     5101 cggcggcgag gaggcgtagg aaatgattca gctcaggacg gttctcaatg ttgcagacaa
     5161 ttccggcgcc aaaaagatca tgtgcgtcca tgtcgtgggt ggcagccaga gattcgtggg
     5221 ttcggtcggg gatgtcatcg tcgcctcggt gaaggaagcg atcccgaaca gcaacatctc
     5281 gaaagggcaa gtggtcaagg ccgtgattgt caggaccagc aaggagatca ggaggaaaga
     5341 cggttcgtac gtccgtttcg atgacaatgc cgcggtcatc atcgacaaca acggggaccc
     5401 gaaggggact cgcatctttg gacccgttgc cagggagctt cgcgagaaga aatatatgcg
     5461 gattgtctcg ctggcccctg aggtagtgta ggaggctctt gtcatgggaa agatgagaat
     5521 aaagaaaggc gaccgggtcc atgtcatttc cgggaaggac gcggggaagg aagggaaagt
     5581 tcttcggcac atcccctcga aggacatgat cgtcgttgaa ggggtaaacc ttgcgaccaa
     5641 gcacgtccgt ccgacccaga agaacccgaa gggcgggatc atcaagcagg aaaccccgtt
     5701 ctatgcggca aaggccatgc tcgtttgccc tgcatgcgga aagcccacgc gcgtcgcccg
     5761 agcgttcatg gacgacgggc gaaaggtgcg gctctgcaaa aagtgcgggg aaatcatcga
     5821 caaggtgtag ggaggaggct tctttgacca tggttccgcg tttgcagaca aagtataagg
     5881 aagaggtcgt cccgcgcctg atgaaggagt tttcctacaa aaacgtcatg gagacgccgc
     5941 gcatcgagaa ggtggtcgtg aatatcggtg ttggtgaggc taaggtcgac atcaagtaca
     6001 tggatgcagc catcaacgaa ctggcccaga tcacggggca gcggcccgtc ctgaaaaggg
     6061 ccaagaaatc ggttgcgggg ttcaagcttc gcgaagggat gcccgtcgcc tgcgcagtca
     6121 cccttcgggg tacgaaaaaa tgggagttcc tggaccggct tatgtccttg gcccttccca
     6181 ggatcaaaga cttccagggt gtttcccgta aaggctttga cggcagaggg aacttcaacc
     6241 tcgggctgaa ggagcagctg attttccccg agatcgacta cgacaaggtt atccgtgtcc
     6301 gcggcatgaa catcacgatc gtcacgacgg cgaaaagcga cgaagaggcc tacctccttc
     6361 tcaaggagct gggtatgccc ttcagtcgtt aaggaggtca ttcaagatgt cgagaacggc
     6421 ccttgaagtc aaggcgaaga gggaaccgaa attcaaggtg cgcaaggtga atcgatgccc
     6481 tctttgtggg cgcgttcatt ccgttttgcg caagttcaac atgtgccgtt gctgcttccg
     6541 caagctggcc cgtgagggga aaatccccgg cgtggtcaag tcgagctggt agcggctaga
     6601 gcagaaggag gcagaggaaa tgtacgtgac cgatcccata gcggatatgc tcacgcgcat
     6661 ccggaatgcg aatacggttt tccaggaaac cgttgatatc ccgatgagca aaatgaagct
     6721 cgagatcgcg aagatcctca aggaagaggg gtatattcgc aatttcaagg ttctgaacga
     6781 tgccaaaaag ccgatttcga tgctcaggct agtgctgaac tacgggccga accgtgagcg
     6841 tgtgatccag gggcttcgta gaatcagcaa gcccggtcgt cgaatctacg tcaacaagag
     6901 tgatttgccc aaggtcatgg gagggctcgg cgtggccatc atttccacct cacaggggat
     6961 gatgacggat gccacagccc gtttcaacgg cctgggaggc gaagtcatct gctacgtttg
     7021 gtagtggggg gtgtagtcct tgtctcgaat tggaagaaaa agcattccgc tctccaagaa
     7081 cgtccaggtt tcggtggagg gacggcacgt caaagtcaag ggaccgaagg gcgaactttc
     7141 gatggacctg ctgccgtgcg tggatgtctc gctcgaagaa ggccagatcg tcgtcacccg
     7201 caaagacgac gagattaagt cgagggcgtt ccacggcatg acccgtgcgt tgctgaacaa
     7261 catggtcgtg ggggtcagtg aaggtttcca gaggcaactt gaaatcgttg gggtcggata
     7321 cagggcccag atgcagggga aaacgcttgt cctgaacctg ggctattctc acccgatcca
     7381 ggtggaaccg ccagctggca tcgagatcca gaccgacggc ccgacgaaga tcctgatcaa
     7441 gggcgctgac aagcaggcag tgggacagct cgcggcaaac atccgcggtt tccgtccgcc
     7501 ggagccctac aaggggaagg ggatccgcta tgcgggagag ttcatcattc gcaaggccgg
     7561 taaggctgga tcgaagaagt agccgaggtg aaagagcatg attagaaaac gaagccgaaa
     7621 cgagatgcgg ttgatccggc atcagcgcct ccgccaaaaa ctttcgggga cggcagaaac
     7681 gcccaggctt tccgtttttc ggagcctgaa gcacatttac gcccaggtca tcgatgacga
     7741 gaagggcacc actcttgtgg ccgtttcgac ccgcgagaag gccgttgcgg aacagctgaa
     7801 gggaaccggg aacatcgaag cggcgaaatt cgtcggcaag ctggttgcgg agcgagccct
     7861 tgaaaaaggg atccaggctg tcgtcttcga ccggggcggg catatgtacc acggtcgggt
     7921 caaggccctg gccgattccg cccgcgaggc cgggctgaag ttctaggagg gaagggccga
     7981 tggcagccag aatgcagaaa gcaacagaga acgagctgac cgaacgagtc gtttgcataa
     8041 agagggtcag caaggtggtt aagggtggca agcgcttcaa gttcagcgtg ctggtcgtcg
     8101 ccggagatgg gaacgggcaa gtcggcgtcg ggatggggaa agcccgggag atccccgagg
     8161 ccgtgcggaa ggggattgaa aaagcaaaga agagcatggt gcccctgaag aaggtgggca
     8221 acaccgttgc tcatccgatc atcggacgct tcggcgcggc agaggttctt ctcaagccgg
     8281 ccgctccggg aacgggcgtt atcgccggcg cggtcgtccg ggccatcatg gaactgggtg
     8341 gagtgaaaga tgtcctgacc aaggtaatcg gaaggacgtc caatcccatt aacgtggcct
     8401 acgcgacctt cgaagccctt cgcgcgatga ggactccgga agagaccctt cggcttcggg
     8461 ggaaggaccg gaagcccgtt caggcggaat aggaggcccg aacatggcaa agctgaggat
     8521 cacctggaaa cggagttgca tcggacgtcc cgacaagcag gagaagacaa tccgggcttt
     8581 tgggtttaag aagctgaacg acacggtcga gatggaagat tcgccccaga ttcggggaat
     8641 gatcgaggca gtccatcacc tagtcgactg ggaagccatc gagaaatagg agggagatcc
     8701 catgcacctg catgatctca gtcccgcccc gggatcccgg cgcaagccca aaaggctcgg
     8761 gtgcggagtt ggaagcggta cagggaaaac ggccggaaaa ggacacaagg gacagaaagc
     8821 ccggacgggc ggcggtgtcg gccgcggttt cgaaggcggc cagatgccga tccagaggag
     8881 ggtccccaag cgagggttca acaatgcccg gcacgcggtg acctacgaag tggtcaatct
     8941 ccagtcgctt gaggagcggt ttgaagccgg tgcggaaatc accgccgtgg aactcctttc
     9001 cgccggcctg attcatggga accgaaacgg tgtaaagatt ctcgcaacgg gcgagctgac
     9061 caaggccttc aacgtcaagg ccagcgcttt cagcgcaaag gccgccgaga agattgctgc
     9121 tgcaggcgga aaggcagagg tgatctaggt gctcgactcc gtccgcaacg cgatgcggtt
     9181 gcctgacctg aaacgtcgga tactcttcac tcttggcgtg cttttcgttt tccgcctggg
     9241 ggctcacatc ccgacgcccg ggatcgacgg aaccgcaatg tcgaggttgt ttgaacaggg
     9301 cggggtgtta ggtttcctgg atctttttgc gggcggggca ctccggcgat tcagcatttt
     9361 tgcgctcggc gtggggccct atatcaacgc gagcatcgtg atgcagcttc tggttgtggt
     9421 gtttcccgct ctggagaagc tccagaagga gggagaggaa ggccgtaaaa agatcgtggc
     9481 ctacactcgc tggagcaccg ttggtttcgc cgcggttcag gctctgggac tctccttctg
     9541 gctgagaggc ctggggatct tctcgggtac cccctgggac ctgcttctcg tggtggcgac
     9601 cattacgacc ggttcgatcg gagtgatgtg gctgggagaa atcatctccg accacgggat
     9661 cggcaacggg atcagtctcc tgatcttcgc ggggatcgtg gcccgcatcc ccgaggcaat
     9721 cgtgcagacc tggaccagcg tcagctccgg ccagatgaat ttcctggtcc tgcttctcgc
     9781 cctcgtgctc atggtcgcgg ttgttgcagg ttgcatcctg ctacaggaag ggcaacgccg
     9841 gttgccggtc cagtacgcga agcgggttgt cggaaacaag gtttatggcg ggcagagcac
     9901 ctttatcccc ttaagggtca accagtcggg ggttattccg atcatctttg cttcgtcggt
     9961 cctgctgctg ccttacacga tcgcgcgttt ctttccgggt agcgcagggg ctttcatcca
    10021 gcgaaccttt tcgccgaaca gcctcgtcta catggtgctg tacgtcgctc tgatcatttt
    10081 cttcgcctac ttctacaccg cggtcgtctt caacccggtg gacgtggcaa acaacatgaa
    10141 gaaatacggt gggttcatcc tgggaattcg cccgggcaag ccgacctccg attacatcga
    10201 aaaggtgatg atacgaatca cgctggccgg ggcgattttt ctctcgctga tcgccctgtt
    10261 gccaaacctg atgacccaga tcatgggaat caacacgttc tatttcggag gaacggcgat
    10321 tctgatcgtc gtcggtgtgg ccctggacac cgttcagcaa atcgagggac agcttttgat
    10381 gcgccattat gagggcattc tgaagcgccg cgacagggcc ggactgttca agctgtgagg
    10441 acgaaagcca tgcgccttat ctttttggga ccccccggat cgggcaaggg aacccaggct
    10501 gcaaaactga tcgaaaaggt ccaggtggcg catatctcca cgggagatat cctgagggag
    10561 aacgtcaaga tgggaacggc gctcggtgtg aaagcaaagg aatttatgga cgccgggaag
    10621 cttgtcccgg atgatgtgat tattgaaatg atgagacaga ggcttcagaa agatgactgc
    10681 tcaagaggat tcatcctcga tggctttccc agaacggtcg ctcaggccga agccctggaa
    10741 aagatgctga acgaactgga catgaagctg gatgcggtga tccttttcaa agtgtccgac
    10801 gaggtcgtag tggagcgtct ttcggggcgc cggggatgcc ggcagtgtgg agccatttat
    10861 cacatccggt ttaaccccag cttacgggga gattcctgcc agatctgcgg aggcgagttg
    10921 taccaaaggg acgatgaccg ggaagaagtg atccggaaac gcctggatgt ctatcactcc
    10981 cagacggctc cgctcgtcgg ctactacgga gaaagagggc tgctgctcga ggtcgatgcc
    11041 ggcaggtcca gcgacgaagt cttgaaaacc attctggacg ccccctgggg aaggtcagaa
    11101 tgatcacctt gaaaaccgaa gaacaactca cacggatgcg cagggcgggg cagatcgtgg
    11161 cggacaccct tcggctcctc agggagcatg tcaagccggg ggtggacacc tacacccttg
    11221 acgcaatggc ttatgaacat gtcgtcaaat gcggagggcg ccccgccttc aagggatatc
    11281 gagtccccgg tatccggagt gcgtttccgg gaacgctctg catctccgtc aacgacgagg
    11341 tcgttcatgg gatcccggac aggactcgcc gactagaaga aggcgatatc gtcagctttg
    11401 acatgggcgt cgagtttggt ggatactacg gagatgccgc ctgcacctat ggggtgggga
    11461 acgtttcgga gacacggcgc agattgatgg aacacactct cgaatctctc catcgggcgc
    11521 tcgcggttgt tcgggatggg gcaaccgtag gtgatatcgg atatgccgtg gagagttata
    11581 tcgtccccat gggctatggc ctggttcgga gttacgcggg gcacggaatc ggcaggaagc
    11641 tgcatgagcc ccctcagatt ccaaattacg gccggccggg gagcggggtt accctcaaaa
    11701 agggaatgac cctcgcaatc gaaccgatgg taatgacagg cggcgaggaa gtcgtcacgg
    11761 ggaaggacgg ttggacggtc ctgacggccg atcattccga tgcggctcat tttgagcaca
    11821 cggtcctggt ttgcgaaagc ggttcggaga tcctcacgcc atgggagagt tgatctcctc
    11881 gaccggagta aaaatggaca gaatggaccg gattgccgct tccgggcaaa gggattccgt
    11941 agcgaggagg tcgattgatc cgcatggcga aggatgatgt gatcgaagtc aaaggcaggg
    12001 tcgttgaacc gctccccaat gccatgtttc gggtggagct ggaaaatggg catcgtatcc
    12061 ttgcccatgt ttcgggaaag atgaggatgc attttatccg tattcttccg ggcgaccagg
    12121 tgctgatcca gctttccacc tacgacttga cacggggtcg gatcgtgtat agatataaat
    12181 gatcgcgaga agccccttcc tgaattaaga ggaattgaat cgttgagaat cgaatgggag
    12241 tgtgaggaaa tgaaggtcaa gccttctgtg aaaccgattt gcgaacattg ttcagtgatc
    12301 aaacggcacg gcgttgtgcg cgtgatttgc agcaagaacc cgcggcacaa gcagagccag
    12361 ggaccaagga ggccatagga cgatggcgcg tattgctgga gtggacttgc cccgggaaaa
    12421 gcggctggag gttgccctga cctacattta cgggattgga cccgcgaggg ccaaggagat
    12481 ccttgccgcc acgggtgtga acccggacac gcgggtgaag gatctgacgg atgacgagtc
    12541 gaagcgactc cgctccgaaa tcgagtccaa ttaccgggtt gaaggagatc tccggaggga
    12601 agtcgcgatg aacatcaagc ggctgatgga catcggctgc tacaggggac tccgacacaa
    12661 gcgcggcttg ccggtccggg ggcagaggac gaagacgaat gcacggaccc gcaagggacc
    12721 gaaaaagacg atcgccggca agaaaaaggc gacgatgaag aagtagcacg gcgaggaggt
    12781 cgacccaatt ggcaaagcgg gttcagaaaa aagggaagcg caaggaaaag aagcacgtta
    12841 gctatggagt ggctcatatc cactccacgt tcaacaacac gatcgtcaac attacggaca
    12901 agcagggcaa tgtcctctcc tgggcttcgg gcggcaacgt ggggttcaag ggggccagga
    12961 agtcgacgcc cttcgctgct cagattgcgg cttcccaggc agcgaaactg gcgcaggatc
    13021 acggcctgat ggaaatcgat gtcgtcgttc agggccctgg acccggccgc gaatcggcaa
    13081 tccgttccct gcaggcagcc ggtctccagg ttcggctgat caaggacgaa acgccgattc
    13141 cacacaatgg ctgccgtccc ccgaagcggc gacgcgttta gcgaagacga aggtaaggag
    13201 ggaacctaca cccgatgagc agatatgtcg gaccagcctg caggcagtgc agggctgaag
    13261 gagtcaagct ttttctcaag ggcgaccgtt gctacacgga gaaatgcgcg atcgtgaagc
    13321 gcaacaagaa accaggccag cacggtgaca gcaaacggca tgccaaactg agcgaatacg
    13381 ggttgcgcct ccgggagaag cagaaactcc gccgctacta cggaatgcac gaagcccagt
    13441 tccgccggtt ctatgatatg gcgacgaaga tgggcggaca ggccggccac aacttcctgc
    13501 agcttcttga aaggcggctg gacaatgtgg tctaccgcac gggttttgcc gtgagccgga
    13561 gacaggcgcg gcagctcgtc tgccatggtc atttcgaggt gaacgggaag aagatcgata
    13621 ttccaagcta ccttgtcagg gccggggatg ttatctccgt tcgggagtcc agccgggata
    13681 tcggatacat caaggaaaac gccgaggtcg cagggagccg ttccgtgccg gcgtggcttg
    13741 aggtgaacgg agaaaacctg agcgcaagga ttgtcgccct ccccaaccgt gatcagatcg
    13801 actcgcctgt caacgagcag ctggtcgtcg agttctatgc ccgctaacgg gtagaagggt
    13861 ggggggagga tggatcccat gcgaccggag atcaaactgg aggagagtac cccccgattt
    13921 ggccggatcg tcgttgagcc tttggagagg gggtacggcg tcactctcgg gaacgcgctg
    13981 aggcgggtcc tcctgtcgtc cattaaaggg gctgcgatca cggcggttcg gatcgaagga
    14041 gtccttcatg aatacagcac ggttccgggg ttgaaggaag atgtcctggg cctgatgatc
    14101 aacctgaagc atatccctct gcgatgccat agcccggagg ctcgtatcct ccatcttgaa
    14161 gtggaaggac cgaaggaaat cacggctgcg gacattcagc cggatagcga ggtcgagttc
    14221 gttgatccct ccagcgtgat cggcacgctg gagaaagggc acaggcttgt gatggacctt
    14281 tacgtggaac aggggatggg atatgccaca acggatcgtc ccagaccctc ctatctgccg
    14341 atcgacgcgc tgatggtgga tgccatcttt tcgccgatcc agcgggtgaa gtacgaggtc
    14401 cagaacgccc gggtcgggca gagaacagac tacgatcgcc ttgtcctcga ggtctggacg
    14461 aacggggtgg tttcggtccc ggaagcagtg agcgaggcct gcaacatcct gaaggggtat
    14521 ttcgcccttt tcgccgagga ggttcggttg ccggaaggag aaattcctgg ccggaccggg
    14581 caggaggagc cgcagcggcc ggaggactcc catccggagg acaaaacagt tgccctggcc
    14641 gacggggatt cgatcctggc cagaccgatc aaggatcttg agttgtcggt cagaagcgaa
    14701 aactgcctgt tgcgtggcgg ggtccacacg attggcgatc tggtgagcaa aagccgatcc
    14761 gagttgatga agatacgcaa tctgggcaaa atctccctac gggaaatcga ggaaaaattg
    14821 gagaaatttt cgatttccct gccgggggat gaggcatcgg atgagccttt gaaggaggac
    14881 tagtttatga ggcataaagt cgatacccgt aggcttggcc ggtacacgag ccaccggctt
    14941 gccatgctga gcaacatggc ggccagcttg atcctcgagg agaagatcac cacaacactc
    15001 ctcaaggcga aggaactgcg ccgcgtttcc gagaggctga tcaccagggc caagggagga
    15061 acgctccagg atcgccggct ggttgccgcc aggatgaaca acaaggaagc ggtcaagaag
    15121 ctctttgatg aaatctcccc gcgctatgcg aaccggccgg gaggctacac ccggatcgtc
    15181 aaactgggaa accgggcggg ggatgccgcc ccgatggccg ttatcgagct ggttcagtaa
    15241 acgatggaac cggaggctgt gtgcgcgctc cggggcgtga gcttcacgta cccgggcgct
    15301 gccgcacccg cgatccagag cgttgacctt accatccgcc ggggagagtg ggttgccctc
    15361 attggggcga acggttcagg caaatccacg ctggcaaaga ttttgaacgc cctgctgatc
    15421 ccgacgcggg gcgattgttt ttcctgcgga tacaggacgg accgggaaga gcatcattgg
    15481 gcgattcgaa gccggatctc gatggttttc cagaatccgg acaaccagat cgttgcttcg
    15541 gtcgtcgagg aggacgttgc tttcgggccc gaaaacctgg gagttccccc ttgcaggatc
    15601 cgggaaatcg ttcgtgagag cctttgccag gtcggactgg aagagaagcg gagaacgccg
    15661 acctacgccc tgtccggagg tcagaagcag cgtctggcga tcgcaggggc tcttgccatg
    15721 aagccggagt gcctggtcct tgacgaggcg acagtgatgc tggaccctca aggaagagaa
    15781 gatgtcctca agatcctgcg ggacctccac tcgaaaggaa tgaccctggt tcagatcacc
    15841 catcgacttg aggaaatcct ggacgctaca cgggtggtcg ttttgagaaa agggcaaatt
    15901 gaatgggaag gtgaacttcc ggaacttttc gagagggtgg accgtttccc ggagtggggg
    15961 ctagagatcc ctccgcttgt tcgtctgacg gggtcgctga agaaaagaaa acttctgcct
    16021 gagtcatgcc tcccttccgt gaaggagctg ctggaaaacc tatgtccata aaggtcgatt
    16081 ctctctgcca cgtctaccac cccggaaccc cgctcgaaac acaagccctt tccggagtag
    16141 acctgattgt cgaaagaggg aagtgggttg cggtcgtcgg ccaaaccggc agcgggaaat
    16201 ccacgctcgc gcagcatctc aacgcgttgc ttctgccctc ttccgggagg gtggaggtcg
    16261 atggcattcc aacgacggca ggggcttccg aacttcgtga aatccgtaga aaagtcggcc
    16321 ttgtcttcca atacccggaa caccagctct tcgcggaaac cgtccgggaa gagatggcct
    16381 tcggcccgag gaactgggaa ttccccgaag aagagatcac aaactcgacg cggaaagtca
    16441 tggatcttct gggattgccg cacgagctcc tggaccggag cccgtttgag ctttccgggg
    16501 gccagaagcg gcggctcagc atcgcttccg ttctggtttc acgccccgat tacttggtcc
    16561 tcgacgagcc gactgccggt ctcgatgcgc gggggcgaaa cgaactgctc tgccttctga
    16621 aggggcttgt caaggaaggt cttggagtgg tgcatgtcac gcatgatctt gagctggcgc
    16681 tgacgatggc ggaccgcatt ctggttctcg aaaaggggaa agcggttttt tgcggcactc
    16741 cagggtccct gctggaagag cttctagagc ggcctatacg ggggctttgt cttccgcccg
    16801 tcctcgagct ggtttcgggc cttcggagtt atggctattc ggtcccctat acgctggatt
    16861 cggaagaact ggctgactgc ctcgaaaggg aaatccggtc ttgaaatttc tcaatcatct
    16921 cacgctcggg cagtacgttc ctgcaaattc tcccgtccat cggctggatc cgagatgcaa
    16981 gatcctatcc gtcattgcac tcctggcagg catcttcctg atggagcatc cggttgcctt
    17041 tcttgcctgg ggaggattgc ttctccttct gtcacggttc tcgcgcatct ccttcagagt
    17101 ggtccttcag ggagccaggc cggttgttct cctgcttgtt tttacggcag cgatccatct
    17161 tttcctgaca aaaggaaccg tcctctggga gtgggggatc ctttcggtga cgagggaggg
    17221 agtcaccatg gctctccgca tgggattaag gcttttcttc ctggtcctct ttgcgggttt
    17281 cctcaccttg accaccagcc ccatggaact ttccgatgga ctggagagcc tttttcatcc
    17341 tttcgagcgc tttggattcc ccgctcacga aatggcgatg atgatgacca tcgctctccg
    17401 gttcatccca accctttttg atgaaacgga ccggatcctc cgggcgcaaa tcgcaagggg
    17461 ggcagacctg gaacacggag ggctcttccg gaggatgaaa gcctttatcc cggtcctggt
    17521 cccgcttttt gtcatcgttt ttcaacgggc cgaagatctt gcccttgcga tggaagcgcg
    17581 ctgctaccgg gggggaagcg gaagaacccg gatgaatccg cttgtctgga agaaggccga
    17641 taccttcgcc atcgccgcgg tgatcgggat tgtggcgttt ttcgtcttgc tggatttgaa
    17701 aggctgaacc atgcgttatg cgttggaaat cgcctatgat gggcgaccgt tctcaggttg
    17761 gcagaaacag ccgggaaaga agactgtcca gggagtcctg gaagaggtcc tttcccgcct
    17821 ggaaggggcg gatgtccacg tcacaggtgc cggacgaacg gatgcaggcg ttcacgcgag
    17881 ggggcaggtg gcttctttcg aactctccaa accctgggag accgagcgac ttctgaaagc
    17941 gctcgagaac aatctgcccc cggaaatcgg tgtgctccgg gcggcaatag cttctgacca
    18001 gttcaatgcc aggcatagtg cgatctggag ggaatatgcc tatttcctct ggacggcttc
    18061 atattgctac ccccaggtac gacccttcgt gtggcgggtc aggtccggct gggattcccc
    18121 ggacctgctc ctggcctgta aggcccttga aggggagcat gattttgccc ttttttgccc
    18181 gcagagcgaa tgtcccccga actcggtcag gcgagtacta cgggcgggga ttgtccgaag
    18241 aagagaatgg gttgcgttcc ggatcagggc gacgggcttc ctcttccaca tggttcgaaa
    18301 catcgtccag gacctgaaga gcattgcaga cgggaaactc tccgtggacg cgttcaagct
    18361 cagcctgaag ggaagagggg aacaacggtc gaaccgcggg atggcgccgg cctccggact
    18421 gtttctttgg aaggtcggct attctccctc tccctgggag tgatacactc ttgggggatc
    18481 gggatttaag caagaagctc cgctaggggg gttggaaatt gttgggaaaa gacattggaa
    18541 tcgacctggg gacggccacc gttctcattt tccttagagg gaagggcgtc atcctgagag
    18601 aaccctccgt ggtcgcgatc gaccagaaca acggaagagt cctggctgtc ggggacgagg
    18661 cgaagaagat gctggggaga accccaggcc acgtcgtggc ggttcgaccc ctccgcgatg
    18721 gtgtcatcgc caactatacg atgacggagg ccatgttgag gcatttcctg aacaaggttt
    18781 ccactgcgtt gaagatggga tccctcttcc ggcaccgggt catggtctgc gttccgtcag
    18841 gggcgacgga tgtggagaaa agggctgtcc tcgaggcggc aattgaagtg ggggcaaggg
    18901 aagccttcct gatcgaggag gcgatggccg cggcgattgg ggctgggctc gaagtcggcg
    18961 agcctcgcgg caacatgatc gtcgatatcg gtggaggcac gacggacgtg gcggtcatct
    19021 cccttggggg agtcgtcgtc gcggaatccc ttcgaatcgg tggcgatgaa ttcgacgagg
    19081 cgatcgcccg cttcatcaag agggagttca acctggcgat cggcgagcag acggcggaaa
    19141 gtctcaagat ccagatcgga acgtgcaagg tcaagcctca tgacgaagaa gcaacattga
    19201 tcatcaaagg cagggatctc gtccaaggcc tgccgagaca gatcgaagtg accagctcga
    19261 tggttgcccg ggcgatcgag gaaccggtta acgccattat cgccggtatc cggaggattc
    19321 tcgaggtcac ccccccggaa ctgtccgccg atatcatcga caggggcatc tggctgacgg
    19381 gtggaggagc cttgctcaga gacctggcag aacgcgtttc agaggaaacg gccatgcctt
    19441 gtcacgtcgc cgagtctcct cttgagtgcg ttgccctcgg gactggaaaa gctttggagt
    19501 cgcttgacca gctaaaggct tccggcgcca tcctctcggt catccgcaaa ggtctcctga
    19561 agggacgcta gggacccaga aagtcgaaga agcaagaaac gggaaccgat gattgaaccc
    19621 ggtaaaagcg aaggtttact gttttcctgt atccagtgcg gccgctgctg caggcggaat
    19681 gggattgttg ccctgacggc gcaggaaatg gagacgatct cggcattcct tgggcttccc
    19741 cccgacgaag gattgaggcg ctatgccacc aggcaggaag ggcgcctcgt tctccgggac
    19801 ggtcctcgag gggaatgcat tctttacgaa tgggcgaccg cccgctgcag ggtctacccc
    19861 gtcaggccgg agcaatgccg gaaatacccc ttctggccat ccatcctgca gagccgggag
    19921 aagtgggagg aagaggggaa acggtgcccg gggatcggca aggaaaagcc gtccctttcc
    19981 gaagtcaccc ccgtggtttc ggaggaagaa gacagaaacg atcttcccag aaatgacctc
    20041 aaaccctaga cgagtttaca ggggttgtga tattataaac aagctgggtt ggtttgttac
    20101 ttctcccaaa atccccgaca aggaggaaaa cagaatgtcc tacacgtgcg gccgcaactt
    20161 caacgtcacc ccggatgagg ttttcccgac gatcgagacc actctcctgc gagatggatt
    20221 cgacatcatc attgacatgg aaaaatcgaa gggaagccac atcgtaaacc tgaaggacgg
    20281 gtcggaatgg ctcgacttct atacgttttt tgcttcggct ccgttcggca tgaaccatcc
    20341 catgctcacc actgaagagt tcaaggaacg gatcttcaga gccgccatca acaaggttgc
    20401 aagctccgat atctacacga tggaaatggc cgacttcatg aaaacgttca acgaagttgc
    20461 caaacccgag ggattcaacc acacgttttt catcgactac ggcaccctcg cggtggagaa
    20521 caccttcaag acgtccatgg actggaaggt ccgcaaactc ctgaaggcag gcaaggtcag
    20581 caagggagac gccatcgacg gcaagaaggg aaccaaagtg atccacttcc gcgacgcgtt
    20641 ccacggccgc agcggataca ccctttccac gaccaatacc tatgatccca acaagtacca
    20701 gtatttcgcg aagttcgact ggccccggat cattcccccg aaaatcacgt ggccccttga
    20761 gcagaacctc gccaatgtgg aatggctgga ggaagtcgcg gtcagccaga tcaaaaaggc
    20821 catctggagc aaccccgacg acatctgcgc cctgatcatc gagacgatcc agggagaggg
    20881 cggggacaac cagttccgga ccgagttctt caagcagctc cgtcagatct gcgacgagaa
    20941 cgagatcatc ctgatctttg acgaagtcca gtgcgggatg ggcatcaccg ggaagatgtg
    21001 ggcatggcag catcatgcgc cggttaagcc agacattttc agcttcggca aaaaggccca
    21061 gatctgtgga ttcgtggctg gtccgcgctt tgacgaactg gaggagaact gcttcacggt
    21121 ttcgagccgg atcaactcca cgtggggcgg aaacgtggtt gacatggtca gggcccagcg
    21181 ctacctcgag atctaccgcg aggagaagat tcttgactat gtcagcaatg tcgcgggccc
    21241 gcgccttctc aacggcctcc tcgaaattca gaaggaattc cccgacatgg tcaggaacac
    21301 gaggggcaag ggcctcatgt gcgctttcga cctcgtgacc cacgatcttc gggacaaggt
    21361 tctccgtgcc tgctgggaag aaaagatgct catcctgccc tgcagcacgc agtcgatccg
    21421 cttccggccg gccctgaacg tccccaccga agacctggac aagggactcg agatcgtccg
    21481 caaagcactg aaaaaggtca aggggtgctg ctgctagaag ctagaccgat cggagggaaa
    21541 tcctccttct agaagaaacg aatcgttcag ggggagcggc gttgccgctc cccctgaacg
    21601 attcgtgtag acgcttaaaa gcaggggatt cgactgattt tcagttgggg atttttatgg
    21661 taggctttag ccgttttaaa aaaatccctg taaaaaaagc gagacttgga aagaggacgg
    21721 gaaattgcgg gacttccttg gagaattcaa ctggatgctg atcctttccc tgattgtagc
    21781 cagcgcgatc gtcgcctatg tcggcgacgt cgttggaatg aggatcggaa agaggaggat
    21841 ttctctcttc ggacttcgtc ccaggtacac ctcctccttc attaccgtgc tgacgggcat
    21901 cctcattacg gtggtgactc tctggcttgt ctccatgaca tctgaaaccg tcaggacggc
    21961 ccttttcagc atgaaatacg tccagaggca gatcacccag ctgacctccc aactccagga
    22021 aagccgccag gaacttcagg aaatggaatt taagcttttc gccagccagg acgaactcca
    22081 gaaaaagcag gcggaattga aggaggcgca ggggaagttc gaaaaagtcc aggcccagtt
    22141 agcgcaggtc cggaaagacc tgaagcttca ggttgaacgt tctagggcag tcgaagccga
    22201 acgagtccgc cttgaaggag aattgaaggg gctggaaaac aggcgcgcct cgctggaaaa
    22261 atccgtctcc accctcaagg ccgagatgga atcgctgaag aggggactgg agcaggttcg
    22321 cgaaggtcaa atcatcgtcc tggccaagga agtccttggc caggtgccgc tgtccccaaa
    22381 aagcagcagg gaatcggttc ggcaggcgct tcggactttg gttgaacagg cgagagccga
    22441 attggcattc cggtcgggtg tgaagccgga acaagttcag atcgtgacgg agaagggaaa
    22501 cgagcttgaa aacccggatc cttttgcggg aaaaccggac cgacaggtga tccggcttgt
    22561 ggcggaatca aacgcggttc gggaagagcc tgtccttgtg tcactggagt cccatccaag
    22621 ccgtttgatc ttcaagaaag ggcaggaact agggaggaga aaggtcccgg gaccgctcaa
    22681 gagggaagag gctgaaaggg agctgtttct tctcttgcgg gaggtcaatg cattttccgt
    22741 gaaggaaggg gtcatcccgg atcctcttcg gggaacggtc ggcaacctgt cggcagggga
    22801 tttctacggt tcggttgaaa agctcgtgga aagcgattcc ccgtccgtgg tcatcgtcga
    22861 agcggaagaa gacgtgttca gcgaaggacc ggttcgagtt agaattgttc tgaagaaggc
    22921 ttcctaaggc cctatacgag agaggaagga tcgtgaatgc taatcgtagc ttgccaggtt
    22981 tgcggcagcg tgaagatttt gccgggaacg ccggatagtg acgggatggc ccgggctacc
    23041 tggatctgcg aaagttgtgg aacaggccag gttttgcaac tttccgtcag tgcggatgcc
    23101 aggggcggag atttgcggcg aatccttgga gggatgtcat ttgcctgcag tggggatccc
    23161 gttcgcgtaa actaatggcg atgccgctgg agaagggcta ccttttctat gacagcaaac
    23221 tggcggtcaa agccgatgag ctccaggacc tttaccggtt cacgcactgg ggacgcagcc
    23281 ggtccttgga ccagatcgag aggatgcttg ccggaacgag catctgcttt tcggcaaggc
    23341 acgggggtag gctggtcgca ttttgccgga ttctgaccga ttttgttttt cgggcctctc
    23401 tttgggatgt gcttgtccat ccggatcacc aggggaaggg actcggaacg gcgttgatcc
    23461 gctatgcgat cgaacacccg gccatacggg aaatcccgct ggtcgttacc tatacgagcg
    23521 agctggcccc ttttttggcc cagctcggtt tcgagccgaa agaagggctt ttgatgctcc
    23581 ttcgttgtcc gatcgagtat tcctgagcgt gaaaattgcc cagggggatc gtttcctgaa
    23641 agttaggctt tactgccctt caccccgaga accacacggg ggagggctac ttttttgagg
    23701 aggtgcggca ggttgatgaa aaacgaaaag agcttcatca cttctgagtc cgtgacggag
    23761 ggacaccccg acaagcttgc ggatcagatt tcagatgggg tcctggatgc cattctgaaa
    23821 gaggacccga tgggaagggt agcctgcgaa tgcatggtgg caacgggcct tgtgatggtg
    23881 gccggccaga ttaccaccga atgctacgtc gacatcccga accttgcccg gaaaatcgtc
    23941 aaggaaatcg gatacacgcg ggcgaaatac ggattcgatg gagacacctg tgccgtcctg
    24001 accgcaatcg acgagcagtc caaggacatc gcccagggag tggaccgggc cctcgaagtg
    24061 cgggaagcac acatgagcga agaagagatc aaccggatcg gggcgggaga ccagggcatg
    24121 atgatcggtt acgcgtccaa cgagaccgag gaatacctgc ctctcccgat cgccctggcg
    24181 caccgccttt ccagggggct tgcttccctg aggaaggaaa agatcctgcc ctacctccgt
    24241 ccagacggga agacccaggt gacggtcgaa taccaggggg ataagcccat tcgggttgaa
    24301 accgtcgtcg tgtcgactca gcaccacccg gcggtggacc aggccgagat cgaggcggac
    24361 cttgttgaaa ggctgatcct gccgacgatt cctgaatacc tgcgggaaag gaagccaagg
    24421 atcctggtga atccgacggg acgctttgtc cagggcggtc cgctcgccga tacgggactg
    24481 accggaagaa agatcatcgt ggacacctac gggggagtcg tgccgcatgg agggggctgc
    24541 ttctcgggca aggatcccac aaaggtggat cgctctgcgg cctacatgac ccgatacgcc
    24601 gcgaagaacc tggtcgccgc tggactcgcg gagaaatgcc agatccaggt ggcgtatgcc
    24661 atcggagttg cacacccggt ttccctgatg gttcatacct acgggacggg aaagcttccg
    24721 gacgaaaccc tgacccgcct ggtttccgaa gtcttcgact ttaggccggc agcgatcatc
    24781 cgggaccttg acttgcgaaa accccagtac cgtcgtcttg ccgcctacgg acacatgggg
    24841 cgggtggacc tgaatccttt acccgcctgg gagcgactgg accgggtcga acagctttct
    24901 gccgcggcaa aaacggcctg atctcccctg gtcagatgat gaagggcggg ttggagactg
    24961 cagcgaagca agcgtggatc atcgctagcc attttctgtg gccttcaacc tgtccagttt
    25021 gcggaaggat cggaagtgac ggctgcccct cctgcctgga cggccttctc tttgaaatgc
    25081 cgaagcgctg tctttggtgc gggggaggat tcttcccctg cggcattctt tcgcacacca
    25141 ccttcctccg agcggggacc tggcatgaag gggccgcaag ggagctagtc cacctggtga
    25201 agtattcagg ccaccgccgc ctggctttcg aaatggggag ggcccttgcc aggctctacc
    25261 gggaggattc caacgtggca ctggtcccgg ttccgctgca tcgagggtcc agccgttcct
    25321 acaaccaggc ggagaggctg gcgcgaggga tgtcggagga atggggggta ccgcttgttg
    25381 aaggcttgac gtggaagctt tcgtttcctt cccaggccgg tcgcaatgcc ttcgaaagga
    25441 gggccctgcc tcgaggagcc tttaggtggg agggcccccg catggaacgg gacatcctga
    25501 tcgttgacga tgtctgcacg acaggaatga cattgctcag ggccgcggag gctctcaggg
    25561 aagcaggaat caaggtgaaa ggggcctact gctggtcggt aagtccgatg gaaacactga
    25621 acgtaacctg gaagggaaaa gcttccgttg aataggagcc gatggagcct tagggtgtgg
    25681 acgagccgag ttttcgtctg gggatccgtc ctgcttggcc ttgcctgggc gctgcatccg
    25741 ttctttctgc ggggcgtccg gcggcatggg ggggtggatt cagcggttcg gggagggacc
    25801 cccgaacttg ttgttttgtc cttgctcgtt gtttcaggcg ggcttctttt ctggtggttt
    25861 ctggagcgcc ttttctggcg taaatatttc cctacgcagg attctcccga gccccccgcg
    25921 aaaaaggacg ttcacaagtg atggaaacct gcgacctcca cttttttttc ccggcagatt
    25981 tcctcgacgg cttcttcgtt ctcgcagggg taggtacagg ccagcccgca gcttgcggac
    26041 aactcccgtg gcacggggat gatccttgcg gaaattccgc gggccctgca ggccttctcg
    26101 aaaagcaacg ccatatgggt ggtgtcgaac gtcgcgagac attccatgtc gaaaagcctc
    26161 cggtggtggg gatgggattg ttacgtaatt atacctagac ttcggaagtc cgtgagagta
    26221 gaatctatgc ccgtagtccg gaaaaatcca aaggagggtg tctgatggtt tcaagcaaaa
    26281 aagcggtctt gttggcagtt ctctcggctt tcatcctgac gatcgtcgcc ggtgtcgccc
    26341 tggcagaaga gaaaatcggc gtgatcgagc cgcagaaggt cctcttccag cacccgaaga
    26401 tggcgcaggt tcaaaagcag gtgcaggctg tcgttcagaa gaagcaggaa gaggcaaaag
    26461 cggccatcga caaggagcag gacaacaaga agaaggccga gatctaccag acgaagcgga
    26521 acgaggcggc gaccgaagag cagaagctga tggcacctct cttcaaggat atcgatcttg
    26581 cgatccgttc cgtggcgaag gccaaggggc tcacgatcgt tctcgacaag gcccaggttt
    26641 tcatgggcgg gatcgacatc accgacgatg tgatccagga actgaagaag aaattctcaa
    26701 actannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntctttg gcatctccct
    26821 ccatcaccag ctggcctgtt tgcatgacat acccccggtg agaaagcagc agggcctgcc
    26881 gtgcgttctg ttcgaccagg aggatcgtta cgccttcctc gttgattctc ttcagttctt
    26941 tgaaaatatc cttgatgatg atcggggcaa gcccgagtga cggttcgtca agaagcagca
    27001 gcctggggct ggccatcaac gctcgtccga tcgcgagcat ttgctgctcc cctcccgata
    27061 gggtgccggc gtactgcgaa cgcctttccc ggaggcgggg gaaaagagtg taaacccatt
    27121 cgaggtcctt cttgaccttc tccttgtcgt tgaccgggaa ggatcccatc atgaggtttt
    27181 cgtaaacggt cagcggtgcg aacactttcc tgccctccgg ggaaagggaa agccctttcg
    27241 tcacgatctg gaaactttgc ccctccaggg gcttcccttc aaagaggatc tttccgcttt
    27301 gccttttcac gttgcccata atggcgttca tgagcgtgga tttccctgca ccgttcgagc
    27361 cgatgacgca gacgatttcc ccgggataca cgtccaggtt gaaacccctg agggctttga
    27421 tcgccccgta ggaaacgtgc aggtctctca ccgacaggag cggctgcttc tggtcctggg
    27481 cttcggtcat gcttccaaca cctcctcgcc gaggtaggcc ttgaggattt cggggtggtt
    27541 ctggatatgg tcaggatcac cctcagcgat tttggcgccg aaattcatgc agatgatgtg
    27601 cgggcagatc tccataacca gttccatgtg gtgttcaatg acgaggatgg tcaagtcgaa
    27661 atcgcgatga atgctcgtga tcaggccatt cagttcctgc acttcatcgg ggttcatccc
    27721 tgccgcgggt tcgtccagga ggagaaggcg gggctgcagc gagagtgccc tggcgatctc
    27781 caggcgacgc tggtgcccgt agggaagagt ccctgctgcc tgctctgcaa gagaatccag
    27841 gccgacccgc tcaagcaggg ccatgctttg gtcgcggatc gacttttcca gggatttcca
    27901 gcggcccagg tgcgtagccg cttcgaagaa accgtactgg taatgctgct ggcatgccgt
    27961 catcacgttc tcgagaacgg tcgatctcgg gaaaagccgc aggttctgga aggtccgtgc
    28021 gatccctttc cggatcacct cgtaggtctt gtagggagtg atgtcctctc cgcagaaaag
    28081 gatgcttccg ctgtcaacat cgtagactcc cgtgatcagg ttgaaaatcg tggtcttccc
    28141 ggctccgttc gggccgatca gtccggcaag ctcccctttt tcgagcgaga agctcatgtc
    28201 ctggacagca tgaacccctc caaagtattt gttgacgtga tccagcttga ggacggcttc
    28261 gctcatcgcc cgtcaccccc ttccgcggtc ctgggtgaac gatccttccg gttgaaaagg
    28321 gatcgaatga agcgtgggct cagttcgtgc tgccccatga ttccctcggg tcggaccacc
    28381 atgatgatga ccatgacggc accgtaaatc aacatcctgt actgggagat cggccggaat
    28441 agttccgtta ccagggtgat gacggcggta ccgatgaggc ttccgctcaa ggaaccgagt
    28501 ccgccgaaaa cgacaaccgc ggtcagctcg gtggatttgt tgaggtcgaa catgaccggt
    28561 tgaatgaagg tcaagaatcc tgccatcagg gcaccagcca cgccgcagta gaacgcggag
    28621 atggaaaggc tcaggacgcg gtagcgggcc gtgttgaacc cgagaagcga ggctgccacg
    28681 taatcgtcgc ggcaggcttt gaaagccctt ccgaaattgc ttcggatcag gaagaacatc
    28741 aggagggcca tgatcacaaa aaaggtgacg gcgaccggca ggttcgtgta gttgtcgatt
    28801 ccggggtatc ccctggcacc gcgggtgaag gactgccagt tttccaggat gagccggatg
    28861 gactcgccga ggccgatgga ggcgatggcg taatagtcgc ccatgagccg cagggtcggg
    28921 acgccgatca ggtaggcgac caccatagcg atgatgcctc ccgcaagaat cgccggcaac
    28981 cagtgaatcc cgtaccggac cgtcatgatg gctgccgagt aggcccctat ggccatgtag
    29041 ccggcatggc cgagggtgaa gacccccgtg aaacccgtca gaagggaaac gcccatggcg
    29101 gcgatcccgt tgatgaaaag gagaatgatg atcccttcaa catagccaga catgggaggg
    29161 cctcctacac tttttcgctg acctgtttcc ccagaaggcc ggaaggcctc cagagaagtg
    29221 tcaggaccag catgccaaac acgaccaggt ctcgcatctg gctagagata aaacccgcgg
    29281 tcagcatttc cgccagaccg aggatcaggc ttcccagcac ggctccggga aggcttccca
    29341 accctccgat gaccgcggca acaaaagcct tgatcgtgat gaagcccagc tggggataga
    29401 gcgtgtaccg gacggacaaa aagatgcctc cgaccgcggc gagtaacccg gcgacaaaga
    29461 aaacgatcga gatgagccgg ttcacgttta cgcccatcaa tccggaggtt cgcagatcat
    29521 aggatgcggc ccggatggca aggccccatt tcgtcttgct gaggaagagt tgaagtgccg
    29581 ttaggaaaag gacagccatt ccgagggaaa ggaggtcgaa ggcgcttgtt gtcgcaagcc
    29641 cgaagaaatt caccgggtcc gtggggatga cggggggaag ggcccggaaa cggcctccga
    29701 cggtgacgac gaagatgttt tctatcacga tgctcatgcc catggaggcg atcaagaggt
    29761 aaagggtgac attggtccgt tcgcggattg gcttgtaggc gagccgctca accaggattg
    29821 ccaccacgcc ggcgcccacc atggccaaaa ggcctgatag ccagatgttg tggcccattg
    29881 cggccagagt gtaataacaa atatatcccc cgataacaag aaagccgccg tgagcgaagt
    29941 tggagaaaag cagaatcgag taaaccagcg aatagccgac ggcgatcaat gcgtagaccg
    30001 atccaaggga aaggccgtta ataatctgtt ggatcaatgt gtccaagtgg ccaacacctc
    30061 acttgccgaa caggatatcc ctcaggtcaa ggaattcatg aagcatgcga tacagagccg
    30121 ggtcggagaa ttgtgaaggc ggacgcggac taccgcgccc gccttcacga taattcggtt
    30181 tccggagtat tacttgggct gtaccttcgt gtagaagacc gccttgccgc cctttgcaag
    30241 aaggacaacg gccgtcttgt tcatcgggtt gtgagttttc gggtcgatgg acatgacggc
    30301 gtggtgcagc ttcacgtcct tcgttttctc cagggcatcg cgaatcgcgg tgctgtccac
    30361 cttgccggcg cgcttgatcg cgtcagcaag ccagtagacg gagtcatagg cgagaacacc
    30421 gttgacgaat tccttgcact cgtcgttgta tttcttcttg taggaatcga agaaaggctt
    30481 catggcgggg tcagccgggt caacgtggtt gatccagtag ctcccctcca tggaaggtcc
    30541 ggcgatttcc cacataaagt cgccgtaacc gtcgcctcca atgaacacga tgtctttcat
    30601 gccgagctca cgagcctgct tcatgatcag agccatttcc ttgcccatgt tcggaaggac
    30661 aaggacctcg gctttgctgt cgcggattgt ggtcagctgg gcgcggaagt ccacgtcggt
    30721 cccggcgcgg aagccctggt cggcgacaac gctcccgccg tatttcttga agtactgcat
    30781 gaagaagtca cgcagtccct gcgagtaatc gctcccgacg tcgtagagga cggcggcctt
    30841 catcttcttg aggtttttcg cggtaaagta ggcaatgacc tgtccctggt agggatccgt
    30901 gaagcagatg cggaacatgt aggggttcag cttgccttcc ggggtcaccg tgacgttcag
    30961 gttcgtggag acggttccga tctggggaat cctgtacttc gtggagaggg gggcggtggc
    31021 gatgttgatc ccgcttccgt tggctccgat gacagcgacc accttgtcat tttcggccat
    31081 tcggcggaaa gcgttcaccg cgtcctcggg acgggtgcgg aagtcatagg ggacaagctc
    31141 gagtttcttg ccaagcacgc ctcccttggc gttgatttcg tcaaccgcga gttttgccga
    31201 gttcaattct gtctgtccat aggcagccca gtcacctgtg agggcggcca ggtacccgac
    31261 cttgatcgtg ttcgcggcca tagccgaacc ggcgaacaca accgccagcg caagtgcaac
    31321 cagcaacagt cccaacttcc tcaaaaccaa ccacctccaa tgattgtttg gaactttcaa
    31381 acgcgcatcg attctatcac aaaacccttg ggcgatggac gtttcagagc aggactcccg
    31441 ctgatgtata agttcccagc tggtacaatg aacctgtctg gagggagggg caggatgaag
    31501 cttttgatta cggatatcga cgggacgctg acggatggga accgtgtccc tgaagaggta
    31561 gcggaagcct gcgggcgatt gagagccaga ggatggaagc tcatgatcgc cacaggacgg
    31621 atctgggctt cggccaggcc attcgcggaa gcgatcggct cggatctccc ctcgatcgtg
    31681 tatgacggag cgagggttct gcaggacggg ggatcgaccc ccctcccctt tagggagtgg
    31741 aagatcccga atcggacagc ggatcgggtc ctggcgcttg gatggggctc ttccctgttg
    31801 atccaggcgt acggggacga agaggtcacc tgcaggccta gcgacgaggt cacgcaacgc
    31861 ttctttcggt ggctaggcgt gcccgtaagt ccggacctga aagacccccg acttgacttt
    31921 gatccttacc ggatcatctt ttacggcgac cccaaggagg cacgggccct gggagaaagc
    31981 atcagggaaa ttctggccac agaggtagag gtcaccctgg cgggagaagg ctttctggac
    32041 attttgcccg ctggggtttc caagggcgcg gcattcaggg cctaccggac aaccgcaggg
    32101 ctcaggcccg aggtggttgt cgctgcaggc gatcacctga acgacatcga ccttctcctt
    32161 gaagccgacc tggccgtcac gttcgaggac gccccgcttg aagtgcgggc tgcctgccat
    32221 ctggtcctgc ctccggcttc ctgcatgggg tttctcgagc tttgccgttt cctggagagg
    32281 ggcgaattcc cccggttttt ctcgaacatc gatagaaagc acaagagggg ttgcccggtc
    32341 tttcttcggg ggcaaccgcc cgaaaccaaa cgggaggtgt gaagatgtcg gcagtcaagg
    32401 gatggcaggt cgaacggatg gagatcccgg aagggtgcaa catcatcctt gggcagagtc
    32461 acttcatcaa aacagtggaa gatcttttcg aggccttggt cacgtcctcc ccggggctgc
    32521 agttcggaat tgccttttgc gaggcttccg aaccctgtct tatccgccgt gatgggaaca
    32581 gcccggatct ggtcgaggcc gcggtgagaa acgccctggc tctctcagcc gggcacgcgt
    32641 tcgtcgttct catccgcaac gggtatccaa tcaacgtcct aaaccgaatc aaggaggtcc
    32701 aggaggtctg cggaattttc gcggccacgg cgaaccccct cgaagtcctc gtcttcgaaa
    32761 cggaacaggg gcgcggaatc gccggggttg tggatggttt ctcctcgagg ggagtggaag
    32821 aggaaagcca cgtcgaggcc aggaagaacc ttttgaggaa catcatcggc tacaagcgct
    32881 agtcgaaaag cgcgtgaaag gttcagtcag gggacggccc cccgcaggcc gtcccttttc
    32941 ttttccagcc caggaggcag cagagagcgg aaacaagaag gccggaaaag agagggttcc
    33001 cgggaaggcg cgttagcggc cacgaaaggg tcagcaccag ccccgccaaa gaagacagga
    33061 aggcccacct ggcggagagt ctccccgggg ccagcagggc gatcgaaaag ggaaggaagg
    33121 tccctgcgcc cctcagcccc atgctcagat aactccagga gaggatgagg gacccgggtg
    33181 ccaggagggc cagaaaggcg gaaagaaaga ccagcgcaag cagggcgagt cggctggtca
    33241 ggagccgatc tttttctccc gcttcttttc gccggagagc ggaggggaga tcgcggacca
    33301 ggttcgtggc gatgccaaga cagagtcccg cagcggttcc gatgatcgtg atggcgattc
    33361 cgctccagag gaccccggcg acaaagggag gaaaatgctg agcaagaaac ttcggcagga
    33421 cctgggaggc gacgagcccc ggttgccgga tcctcatgcc gagtcctacc caaatggcaa
    33481 aaagtccaag aggcggcatc aggacggcgg agagaagggc gcccttccgg gcggtttctt
    33541 cgcttgaagc cgaaaaaacg gcctggatgt agatctgggt ggtcatgact ccgaccagaa
    33601 gggaaaggcc tgcgttcaaa tcgaccccaa ctcccctgcc gaaaaggttg aaccacggat
    33661 ggaagggcaa ggcgttccag agcgggggga gattcctccc ctccattgcg gtgacggcgg
    33721 cgcaaaccgc cagcacgaaa taaagaaaag caattttggc ttttcccagg accccatagc
    33781 ttttcaagcc gccgagaagg atgaacccta ggacggccag tgataccact cctgcggcaa
    33841 ccgtgtccgg gatgggccag attccctgaa tcatggccag gcccgccagg aactgggcca
    33901 ctacggaaac gaaggtgccg agtgtcgagg ccgccagggc gacgattccg gttttcctcc
    33961 cgtactcccg ggcaaggaat tccgggatgg tcgttagtcc cgttcgcctc agggggcgcg
    34021 caaaccagag ccccaggatc aggcatccga ttcctcctcc cagggtgaac caccaggccg
    34081 atagtcccca acggtacgcc atttcgacgg ttccgaccgt gctggcccct cccacgaggg
    34141 caccgagaag gatcccgctg acctcgagag tgccggcgct gcgtcctgcc agggcgtagt
    34201 ccgcgaccga gcggattccc ctgcctgaaa gggttcccaa aagaacaaag gcgagaagaa
    34261 tgatggcggc tcccataccg aaggcaacca aggcgctcgt tcctttcgtt tcgaatctgc
    34321 cgatgtcggg gaattcggcc ccgctgacgg atcgtgggca ttatgttatt tttatcacag
    34381 agaacaccat gctgatgcag accggggagg tcggacttct tgaatctgag gcaaatcgca
    34441 gcttgtatcg gagcggagat ccttcacgga gaacccctgc tcgagaaggt cgagatcgag
    34501 agagcctacg catcggacct gatgagtgac gtgttggcgt ttgccagccc ggggacactc
    34561 cttctgaccg gattgaccaa cgttcagatc atccggacgg cccagatgat ggatattcca
    34621 gccgtggtct tcgtgagggg caaaagacct caggaagagg cggtgaacct ggccaggtcg
    34681 gtcggcatgc cgcttctggt ttcctcgaaa agcatgtacg aaacatgcgg cctgctttac
    34741 gcggccggcc tgtctccctg cgtcatcccc caccgggagg catagcgtgt ggatagccgt
    34801 tatgaagtgg agtttccggt aaggggggac gatttccttg cagcgggaga agcctccaac
    34861 caaatcaagg aaacgctagt catgctgggg gtcccctctg ccatttccag gagggcatcg
    34921 gtcattacct acgaggcgga aatgaacctc gtgatccatg cggggggcgg tatcctgcgg
    34981 gcgatcatca ccccggaaag gttgacgatc gtggcgaccg acctgggccc gggcattcca
    35041 gaccttgcct tggccatgaa ggaagggttt tcaaccgcct ccgaccgagt gcgcgagatg
    35101 ggattcgggg ctggaatggg actgccgaac atcaagcgca atgcggatgt cctggagatc
    35161 gacacggagg tcggaagggg aaccaccttg actgcaacgg tttttttcgg tcggcaagac
    35221 gcacgggagg ggtaggggat ggaaaccggg gtcaagatcg tcgaaggaag ctgcaaagga
    35281 tgcgtcaact gcatcaagtc ctgtccaacc gaagccctga gggtcatcga ggggaaggtg
    35341 aacattctcc ccgaactttg cattgactgc ggggagtgcc tccgaacctg tcgggagaag
    35401 gccctcgccc tcgacgaaga cgattgggat ctcctcaaaa gccacggtcc ggtgacgctc
    35461 atggccgacc ccaccttctg cgcccagttt tcccattact ggagcccggc cctgattcgg
    35521 gaagctttgg cctccctcga aatgcaggat cttttcgacc aggcctccgt cgccttcgac
    35581 cttgccgccc tggctaccgc caaccagctg caggaggcgt ccagggagag ccttcccctc
    35641 atttcgatct attgcccggc ggttgtgcga ctgatccagg tccgcttccc cgagctgctc
    35701 caccggcttg tgaaaacgga aacccccctg gaaacgggga cccttctctg gagaagggaa
    35761 aacgagtcga aaagcggcgt tgtcctggtg accccctgca gtgcgaaggt aatgctggtc
    35821 cgaggccccg tgggaaggac aagaagcacg atggggcacg tcgtttcggt gaagcggctg
    35881 gcgagggacc tgcttgccgc cggccccaag gtggaaggca aatcagccgc ccccgtcgga
    35941 agccgctggc tagcctgggc gctgcgggga ggcgaaagcc ggcatgtccg ggcgttttcc
    36001 gaaaggaagt tgacgacgat ggccgtgtcc gggctcagga acacaattga cctgttgcag
    36061 gacctggaac tcggaagact gaaaggagtg gatttcgtgg agtgccgggt ttgcgatcta
    36121 ggttgcatcg gggggatcgg gaacgcggaa tcccggttcc tttccgagct gagactttcc
    36181 cggcagccca tcgaatggaa ggtctcggca ggggagcatg ctgcactcca ggaactctac
    36241 gaaaccggaa tctggaagct ggagcagccc atccagccca ggcccaggcc ttctttagcg
    36301 gataccctcg acgaggcgat gaagaaattg aaggagatgc aggctctata cgcagaactg
    36361 ccccatatcg actgcggggc ctgcgggcgc ccctcctgca gggctcttgc agaagacgtg
    36421 gtgagaggcg agggcgagtt gacggactgt gtcttcaagt tgcgggaacg gatctccctt
    36481 cttggggagg agattaccct tttaagcggc aaactgccgc atgccttcca cgaaaggaag
    36541 catggcaaat gaccgtttcg tcggttatcg aggcactcgg gctgaccgtc caccacgaag
    36601 ggaaggggaa ttgtcccgtt tcccggggag tggtcggaga tcttttgagt tttgtcatgg
    36661 gcggggcgcc ggaaggtgcc gtctgggtga ccctccagac tcacgtcaat attgcagcgg
    36721 tagcggttct gaaagacatc ccgctgatca tccttgcatc ggggaggaat cccgacgagg
    36781 aacttctcga gcggtgtcgt ctggaaaaca ttggtcttgc tggagcttcc gcttcgccct
    36841 tcgatgtctg cggagccctt cacgacttgg gactgaaggg ataaccgggc ggatgagacc
    36901 ggtccgggcg gatcttcata tccacaccgt cctttctccg tgcggcggcc ttgacatggg
    36961 agcccccgaa atcgttgccc gtgccctgaa atgcggactc gaggtggtcg cggtgaccga
    37021 ccacaacacg tgtcgaaatg cggccgccgt gatgaaagcc gcccggggca aacaactggt
    37081 ggttttcccc ggccttgagg tgcaaagctc cgaggacatc catgccgtcg tgcttttcgg
    37141 gactcttgag gaagcccttg attttgaagc ctggctttgg gaaggattcc cgaaggtatt
    37201 gaaccggccg gaactttttg gagagcaaat tttgattgac gaggagaacc ggattctcgc
    37261 cgaggaaccc atccttttgg tccagggcgc caggcgcagc atcgatgaga tcgtaaccga
    37321 ggccaggtcc cgaaacggag tggttttcct gagccacgtc gaccgagagg cgttctccta
    37381 tccagcggtt ctcggcccga taccggtcga ctatcccgtc gacgccctcg aactgacctg
    37441 ttacgcaacg gaaaaaacgg tggagaactg ccggaaagcc tgcccttcgt ttcccctgat
    37501 ccggaattcg gatgcgcaca ccctggctca gatcgagccc cgttgctcaa cggccttcct
    37561 cgtggaggaa ccggatttcc aaaatcttcg gcgggcgttc aggaatgaag ggggctgtcg
    37621 ggtcctggat ccttggaaag tttaggaatg aaaaagggca acccccctcc ggatagggta
    37681 aaatcgagaa aaggaggtgt ccgccttgct agaagaaaag cccgaagaac tcagcgcccc
    37741 ccagcggaag ctgcagtgct ggtgcggacg aaggatgatc ctggtggaat ccttccccga
    37801 tgatggaacg tttcgttacg cttgcccctt gttgctggca ggggacacga atcacgacca
    37861 actgctcagc gaagaagaat acatcgaaga accggacgag aagccgtagc gtccgttact
    37921 ccggttttcg aatgaaccag agcgccgcat tgtgggtcgc ggcgtcgaag gaagaggggc
    37981 tttcgggaat ggagaagagg ggccccttga cggtcatccc gtaaaggggc agagcacccc
    38041 ccagggtttg aaggcgggca aggatgtccc ggttaacgga gagcgagacg aaaatgacgg
    38101 ttgcctctgc cctgtctgtc gaagctcctg ccacgccgat ccagtggtcg gaagcgagag
    38161 gaattttttc caggtaccgt ccagggccgg cattcccgtt catgtcctgg atcgtcgttc
    38221 cccttttcat ccagacgggc catctcccgt agtcggcatg ataaaggaca gcggcgcttt
    38281 tcatcgaccg catgtcttcc aggatccggg tcgcttcagc cttgttcgtc acggaaaagg
    38341 aaacgagcat cattgctcct gccaggatgc cgacgagaag gaggaccacc aggatctcaa
    38401 cgagcgtgag tgccgtccgt ttctttcggt ttcttcccat tttctttctt cccattttcc
    38461 gaccccgtct ctttttggat ccggccgctt ttcctggaga attcatcaaa accaagcata
    38521 cccgagaagc cgtggcgtcg caatcggcct tccggtacgg tgccgttttt cagttttcag
    38581 ggccgatgct aagatatcgg catgggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    38641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    38701 nnnnnnccgg tacggtgccg tttttcagtt ttcagggccg atgctaagat atcggcatgg
    38761 ggaaaaagcg gaatcctcga gttgaattga gggaaggtcc ggtggaacac cggcttctgg
    38821 aagatctctt caaggccagg gggctgctcc cggaggggga aatccgttcc cctgagggag
    38881 aaacgccccg gcaggaaaag cccgtgcacc agccggatta ttcaatagtc gagcggatcg
    38941 tcctgcggtt cgaaaggaag ggacacgggg gaaaatcggt gacccgcgtc gaaggtctgc
    39001 ctttcgcgga ggaggttctc gaggatctgg cccgcgaact caagaaagcg atgggatgcg
    39061 gagcctgggt ggcgggaaac gagatttttc tgcagggcga cctgaccgaa aggtcgcagg
    39121 cctggtttca gaagaaggga gcaaaacgcg tggtcatctc aaaagcgggt ggattcagga
    39181 aatgaacttt cgggctttcc gggaagaaga tctggctgcg ctcgtcgatg aagtgcgacc
    39241 cttggcatcg gaggggaaag tcgcgaccta tatccctgaa ctggcgcggg gaaaccccga
    39301 gtccctggcg gttgcagcgg tggacgggaa aggatcggaa gtcgccgccg gagacaggga
    39361 tctacggttc accatgcagt ccatatcgaa agtcgtttcg cttgctgtcg ccttatcgga
    39421 gtttggcgaa aaggcggtct tcggcagggt ggggatggat cccaccgctg atcccttcaa
    39481 ctccgtcatg cgcctggaga tggttcgtcc tcatcgtccc cagaaccccc tcatcaacgc
    39541 cggggcgatc gtcgttgact ccatgcttct cgccagggga ccggaggaag ccttccattc
    39601 catccgggaa atggcgaggc gcatgaccgg caaccctcaa ctccgggaaa acgaagcggt
    39661 ctatctttcc gagaaacgga caggcgaccg caatcgttcc ttggcttact tcctgaagag
    39721 tgtcaaaacg atccgcggag atgtggaaga aatcctggat gtctatttca ggcagtgcag
    39781 cctcgaagtg acggtttcag accttgcggt gcttgccggg actctcgcct tcgatgggaa
    39841 aaatcccctg accggggaaa cggtgcttcc cgggaagatc tgccggatcc ttagagcgct
    39901 gatggccacg tgcggtcttt acgacggttc aggagagttc gcggtcaggg tcggtattcc
    39961 ggcaaaaagc ggggtcggag gaggcatcgt ggcttcggtt cccggcgtca tgggaatctc
    40021 agtctgcggg cccgccctgg atcaccaggg gaattccatc gcgggtatcg cgcttttgga
    40081 gcgtttgtcc gatcggttcg gcctgagggt tctctaggcc ccgctgcgct tgaaaaaccc
    40141 ggctgggaca ggaacaccga cccagccggg tcctttgtcc gcctagtcga tttttcggta
    40201 ggcctgcttt ttggccccgc agatggggca ggtatccggt gcatcccctt ccgcgatgtg
    40261 tccgcaaacg gtgcaaagga aaaggtcggt tgtttctcca atgtggaggg tctgcagagc
    40321 tttctcgtag aggtgcgcat gaacggcctc cgccttgccc accagatcca gtttcctcgc
    40381 taagggctcg gcaccctcct gcttcgcatt ctcgacgaat ccggggtaca tcttggtgaa
    40441 ctcgtacgtt tccccttcga tggccgccct gaggttttct tccgtgctgc ggatcattcc
    40501 cagagccttg agatggtcga tcgcatggat tgtttccgct tccgcagcag ccctgaacag
    40561 cttcgcagcc tgatggaacc cttccgcctc cgcctggcgc gcgaaggcga gatacttgcg
    40621 gttggccatc gattccccgg caaaagcttc cttgaggttt tcctccgtct tcttcatctg
    40681 agaaacatcc ctcctgttct aaatttgtaa tgattataaa taaactggac ccgggatgca
    40741 atgcgaaaaa agcagatcct caccgcgagg ccatctccag ggagcggaag aggagttcaa
    40801 ggtcctcatc cttgccttgc aggggaaggg gcaggccgac ggtcctctcg ccgagggcgt
    40861 aggcggaagg gaaggagaat cgaagggcag ggttcaggcg ccagagagaa ggacactcat
    40921 atccggattt tcgcagaaga aagcggatcc ggtttgcatc gaaagcgacc cggaccggga
    40981 acacaagggg tgcggcatcc tctccgacgg agccgaaaag agggcggatt ccacagcttt
    41041 cgaaggccgg ttgggaaagg tggtccagga tgagttttcc gaaaaccctt cttcggtggg
    41101 cttcctctgg aaaattctcc gcgttcagct tttcccagga agccgaagag ggacggcggg
    41161 ggagcgagga aaaggaggct tccaaatctg cttcagcctc ttccaggagg gaaagtcctt
    41221 tctcaaattc ccctttctgg aggtacccaa aagccttctc ccgtttttcc tcgattcgcg
    41281 ggtcaggcgg agccagatcg gcctgcaccg gaaagctcga aacgagggct cctccatcgg
    41341 ggacctggaa gaatttccga aaactgaaca gtgcaaaatc cccttcaagc ccgacgtttc
    41401 cgctcagcga agcgcctgcg caatcttcga ccagaaaagg acggttttcg ggggacctcg
    41461 agcggaacca ggcaagggcc ccatgattcg ggaatccgaa ataatggatg attagcaaaa
    41521 gatcgccccg ggaagggccg gaaccgggaa aaaccggttt aaagccggcg ctgttttcat
    41581 agaaacgcag gcggatcccg cttttcctga aaaccgcggg aagagacgga caggaatatg
    41641 ccgggatcca tgccgtcccg gaaaaaccgt ttccccctag gtgccgaagg gcaagaacca
    41701 aggcggatcg cccgctgtcc gtcgggatga cgattttgcc cttcctctcg tatgaaccga
    41761 gaaacgggtc gactccctct cttccgtgga cgaatttgac cgttcggtcg ctcatggaga
    41821 tatcatagct ctcatcgggc cttttaacga aaggggggtc gatctcccgt atggaaaagc
    41881 cctttgaccg aattgcgccg tattacaacc gatggattgg tcggttcatg tcctcgcgga
    41941 tggaccggat ggccgagatg ctcgcctgtt cacccgaaga gaccgtgct
//
LOCUS       DAOOVN010000008        35015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_809_length_35015_cov_16.220366, whole genome shotgun sequence.
ACCESSION   DAOOVN010000008 DAOOVN010000000
VERSION     DAOOVN010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 35015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35015
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_809_length_35015_cov_16.220366"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1464
                     /locus_tag="PLY83_01910"
     CDS             <1..1464
                     /locus_tag="PLY83_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV28824.1"
                     /translation="LVTPIEDMKAVFDWKDKVPSFYQPPRQEPEPVSADVTSAEVIPN
                     EGSPEENSCVSEDCLEVPPPMNVLLADGMSRRAVESLSREIRQNIFAVGGGKDDTVPP
                     VKKVSGLKPGEAVGVMLAWGDVNIGATGTLTALSNDGRFLAFAHPFLNHGAVAYPLTE
                     AWVHEVVPSIESPFKLGSPLSIIGTVTQDRPQAIGGKLNVYPPSYDFTVRFDDVDNKS
                     VQTKRFHVALAPFLLSKLAPEALTGLLDDLWGRVGAGTARLNLSVTGPNLSGGWSRNN
                     LFFSDKDLTKEVLKELKELMETFALNPFTEIAPLGFRLDLEMTSSPRILLIEDVKLDK
                     KKINPGEKLKVEVRLRPYRKDPFTRTFELTVPKDASGRSMVVVRGGGINEPGQESIME
                     GKTTIRDFPTLLKELSVRETNNQVVVEILASGAPGGKAPIRKGKEAEPADETPELLSE
                     ITEKKLKEGSMKIYNTNYYVDGLQQREISVGTDSAED"
     gene            1472..2248
                     /locus_tag="PLY83_01915"
     CDS             1472..2248
                     /locus_tag="PLY83_01915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOV28825.1"
                     /translation="MEIFRWLGRIAIGFFETLGFFSLFLLKSARGVFRGKWSRSDLSK
                     QLERVGVSSIFVVSITSAFTGMVMAVQTLDQFVRFGATNYIGGVIGLSMVREMSPVLT
                     GLVVTGRVGAAMAAEISSMRITEQLDALQAFGLDEVAFVGSPRLVAGLVMLPLLTIYS
                     FVIGIGGGYLFVVTRGIHSTTFLKSLEALLEPSDIFGGLLKAAFFGVIIATTACAEGF
                     RAGNGAIGVGEATTRAVVWSNMLILAFNYILSTILFGGIR"
     gene            2245..3018
                     /locus_tag="PLY83_01920"
     CDS             2245..3018
                     /locus_tag="PLY83_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOV28826.1"
                     /translation="MKARLLEATAIEKSFGENVVLRGIDLALSAGETLSLMGPSGCGK
                     TTVLRMVVGLIKPDRGKITLFGKEMTDSEDDESVIELKRRIGIVFQGGALFDSLNVGE
                     NVAFPLRYCLGLSDEDEIQRRVFEVLEQVELPGTESLMPAELSGGMKKRIAIARALVH
                     EPDILLLDEPTTGLDPQTARHIDRLVSEIGKRRNLGILAVTHDLVTAIGISDTILLMD
                     QGIISWRGTPEEWKTSEDPNVRRFATGLRPVRAGRTVCP"
     gene            3015..4382
                     /locus_tag="PLY83_01925"
     CDS             3015..4382
                     /locus_tag="PLY83_01925"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOV28827.1"
                     /translation="MNREFKVGLTVFLALLALGGMVFITGGALFRHGGNSYEILFRDA
                     MGLEQGAPAYVSGIESGAVRSLRLSPEGVIVTVILDPSVRIPKDSKILIGTGGLLGKP
                     LLRIERGSSQEFFQPGDRLSGEIPPDFDSVLEEIQKNLKALGATFQNLNEVLGDPERK
                     AKLGKALDNLPDLVEDSRSAMRRIGAAGTEIQELGRLARKQIKLLSDRLQELGGHLDR
                     VVLENREDVRKSIVSLASLLKRADETFAQFDEDRMAGKEMREAIRNLSKAAKGIEALS
                     SSLSEALGDSLPTSEDKEGRINLKETLQGAARIVSSVGSIRTSGEVALHRSVSGEDDN
                     EGWAMDLSLQIGKEHSLWNLLLEGTGLGNGSRVTASALYQTAWGRVWGGLVRGDVGFG
                     AAVDFRSEFPFSLSAEWWDEQGGSWAFRGKWYVKPRWGFYYERLEPGHDPASDFVGVF
                     YQFSR"
     gene            4396..5001
                     /gene="tmk"
                     /locus_tag="PLY83_01930"
     CDS             4396..5001
                     /gene="tmk"
                     /locus_tag="PLY83_01930"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOV28828.1"
                     /translation="MFITFEGIDGCGKSTQARRLVEFLLREKGNGKALWTREPGGWPG
                     SGDLRELILSQEFEHPWTEVFLFLADRCEHAKKIIEPALQEGVIVICERFDDSTRAYQ
                     VWGRGIELEKIERALALAEYPRPDRTILLDVSVETAMGRVLARANLDRYEKEGKSFLE
                     RVIEGYRFLAAREKSRIRPVNGEGKPDEVFARILDSLEGCF"
     gene            5001..5783
                     /locus_tag="PLY83_01935"
     CDS             5001..5783
                     /locus_tag="PLY83_01935"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV28829.1"
                     /translation="MTEHPTNPDQGGENGISLFDAVASSRAWKELSSALEAGVVPQSV
                     VFCSPSLFHDPFVSLYAHTLFSGNGSGWLGETHPDLLRLGTPEAPPGIETFREQLVEL
                     YSSPVAAPYRLAVIHCADRLSPPAANSLLKITEEPPPRGRILFLLEEENLLPTLRSRS
                     WILKMPPEEVLAPAAPPKNDEEWVHWLSEASSWKTDAVLLRATAWARWYALSGQFRKA
                     EELDTLVVLFQKTKLSASMAIDLIFLMTREESTFEDFLDSLR"
     gene            5842..7086
                     /gene="ricT"
                     /locus_tag="PLY83_01940"
     CDS             5842..7086
                     /gene="ricT"
                     /locus_tag="PLY83_01940"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOV28830.1"
                     /translation="MIIIESPRGREIGIVAGPISPSQEERYRKAPTSETADGSSKPGT
                     PTDEVQFIGFPLPDETGFRKECAEEENQVLQRAREILKTHNLPMKLVDVEYLLDRSKL
                     FFYFTAEQRIDFRGFVRDLAREFKTRIELRQIGGRDEAKVAAGISQCGMPCCCSTWLN
                     RFEPICIKMVKQQNLALNPTKISGICGRLMCCMGYEHQTYQELWKGLPNPGTKLKSLT
                     GETYLISGVDLMGKSLRIFCPDRTELLVPVSEFAKFKEAIQKGEVWEPQREEVVPLPF
                     DSLLQEAVTDSDRIVEDGICPVEKPVRQRPGRLISRERAVRRTGERETRERELPAKPV
                     VSTVPAVKSEAPAPEETRTKRRRPRRKKRKPAAAAPEMAPAKPALETRIEASPTGPDT
                     QERPHRKRNSRRRKSRKPQAEE"
     gene            7186..8073
                     /gene="ftsY"
                     /locus_tag="PLY83_01945"
     CDS             7186..8073
                     /gene="ftsY"
                     /locus_tag="PLY83_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOV28831.1"
                     /translation="MTRLISASDENPDFWEEIEGTLLKGDVGVDLSEKLIADFRKLRK
                     NPGMDRCPPVWLFGKQLEAIFAGNPLNGAPLRLNGKPSVVLLAGVNGSGKTTTAGKLA
                     SQFTRAGNRVVLAAADTYRAAAIDQLKTWGDRCGARTISHGMGSDPAAVVFDAIRSAK
                     ADGAEWVIADTAGRLHTRHNLMEELKKVARVVEREVGPGNAEFLLVIDAVMGQNSFQQ
                     AKVFGEALPLTGIVLTKYDNTAKGGVILSVVHDLGLPVRYVGLGEKVEDLALFQPKEF
                     IEALLGLPEGDLCKEGLRS"
     gene            8070..9392
                     /gene="purB"
                     /locus_tag="PLY83_01950"
     CDS             8070..9392
                     /gene="purB"
                     /locus_tag="PLY83_01950"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOV28832.1"
                     /translation="MIERYETEEMKRIWSDENRFQKWLEVELAVCEAWTEEGKIPAEA
                     MDTIRKRAGFDMQRIAEIESSTHHDVIAFVTSVAEKIGPEGRFIHLGLTSSDVVDTAG
                     SLLILEALAVLTETLKSFSGLVLEKAREYRHIPCVGRTHGIHGEPTTFGLKLLNWHSQ
                     LLRDLERLELANKHIRAGKISGAVGTYAHCPPQIEEKVCSKLGLQRAAVSNQILQRDR
                     HAEVLTAIAILGSTMERIATEIRHLQRTEVLEAAEPFSKGQKGSSAMPHKRNPILSER
                     VCGMARLLRGYALASMENIALWHERDISHSSVERVAWPDAFHLAHYSLKTLMRILAGL
                     QVDPGRMAGNLEMTRGLLFSQRVLLFLIEEMGVSREEAYAIVQENAMRCWAGEGHLRD
                     LLSSDPRVSRYLDDDALDLLFDYRYFTRYVDEIFSRFENKGETDPQNA"
     gene            9406..10386
                     /gene="glpX"
                     /locus_tag="PLY83_01955"
     CDS             9406..10386
                     /gene="glpX"
                     /locus_tag="PLY83_01955"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HOV28833.1"
                     /translation="MSLPERNLALELVRVTESAAMAAGRWMGRGDKNGADGAAVNAMR
                     YMLNTIRMDGTVVIGEGEKDEAPMLFNGERLGTGDEPKVDIAVDPIDGTRLLSLGRQN
                     SISVVALAERGSMYFPQHIFYMNKLATGPEAAGSIDIRLPIGENIRRVAKAKKKNVED
                     VTVVLLDRPRHEKLIADIREAGARIRLITDGDIAGALMTCKEDAGVDLLAGIGGSPEA
                     VITACALKCVGGAMYCTLWPRNEEEAERCRELGMDLEQVLSMDDLVKSNNVFFAATGI
                     TNGDLLQGVRYEGDRIRTQSIVMRSASGTIRYIEAIHKLSKLETISGINY"
     gene            10504..10701
                     /locus_tag="PLY83_01960"
     CDS             10504..10701
                     /locus_tag="PLY83_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOV28834.1"
                     /translation="MKGTVKWFNATKGYGFITTEEGKDVFAHYSAIEMEGFKTLEEGQ
                     AVEFEVVEGAKGPQAAHIRPL"
     gene            complement(10752..13019)
                     /gene="hypF"
                     /locus_tag="PLY83_01965"
     CDS             complement(10752..13019)
                     /gene="hypF"
                     /locus_tag="PLY83_01965"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HOV28835.1"
                     /translation="MEKARFLIGGVVQGVGFRPFCARLAMEEKLKGSVRNTSSGVELE
                     LWGERGQIDRYLRRLERECPTAAYIQSVIPLERQDNASSPPDSFEILKSEGRAVRRVL
                     IPPDIATCESCLEEMRDPSDRRYRYPFINCTLCGPRFSIIRDLPYDRSNTTMSKFSMC
                     EECSAEYRDPTNRRYHAQPNACLVCGPRLWACDCRGITLAEDEAAIQTATELISRGSI
                     VAVKGLGGFHLACDARSDDAVGELRNRKRRSHRPFAIMVRDLEAALKVAVLTEKGKDC
                     LMSSRRPIVLCQARSDSGLSPLLNPGLDSIGIMLPYTPLHHLLLEKLEVLVMTSANLS
                     EEPIVADNEEALGSLSSLVDAFLLHNRKICMRIDDSVVSVAGSRAFYVRRSRGVVPQP
                     LFLPKSGPSILASGGEMKATFSITHEGALFPSQYLGDLKSIGGATLYREALKHFLSLY
                     SIHPEFLVHDLHPLYLSTRLCHEVLPNPTETLAVQHHHAHLAACLFENGIDEPAVGLI
                     LDGTGYGTDGGIWGGEILWGDASGFERLGHLVPFTLPGGEKAIWEPWRCGMSLAYASG
                     LNEEAREWAKRKSKLSPDFDRFLEVSIQAGVKTTSCGRLFDGVSALLGFVESVTFDGQ
                     APMVLEAAARGSGNLPFSVVESDGKILIDWRPAIRKLFADRGRKSPASISAAFHGGLA
                     RALGEAAALAAERKGTRRVVLSGGVWQNRRLFSLMIAEARRRGLIPVIHTLLSPNDEC
                     VSVGQAVAGYWKWGK"
     gene            13105..15657
                     /locus_tag="PLY83_01970"
     CDS             13105..15657
                     /locus_tag="PLY83_01970"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOV28836.1"
                     /translation="MSPATDDHPHSVPVEDLLRSLSSDPERGLSQEQAQAALKRYGPN
                     AFAKIKPRPWWSFLLSQFKSPMIGLLALAAVVSLTMGEILDAAAIFTVILLNALIGFF
                     TEFRAEKAMEALRSMTSPNARVVRNGSVAVVSAEKVVPGDLLLLEAGDIVPADARLLE
                     SRNIALDEASLTGESMPVEKNVDCLPAETPLAERLNCVFSGTAVVRGNAKALVFATGT
                     DSELGRISMLLESVKKQSTPLEERLEQFSRFLIVLVGVLAFLVFALGVFQGRGLFKMF
                     ETGIALAVAAVPEGLPFVATMTLAIGVHRMAKQKALVRNLSSVETLGSTTVICTDKTG
                     TLTLNDMTVRLVLPVCDEQERTLLQTAVLCNNAKLEQSGDIGDPMEIALLRAAREKGM
                     NLEEVKMRFPRTGENPFDSRTMTMETFHGVWKALKGAPEKVLSQTNHIWTEEGIRPMG
                     GQDRKHWLERAEEISGQGMRSLAFAWGNEKEGLAFVGLIGIIDPPRPEVKDAVSACHE
                     AGIRVVMVTGDHLATARSIAKEVGILCPLHPDSLEGKELELLDEKSLSSKIAAVSVIA
                     RVAPEHKLKIVQAFQAAGETVAMTGDGVNDAVALKQADVGIAMGIQGTEVSKESSDLI
                     LQDDRFSTIVNAIDEGRRIFGNIRKSVLFLLCCNLSEVFTVLFAVLSRLPAILLPLQI
                     LWINLITDVLPALSLALDPAEPGTMRRPPMPRSERILTPRHLRLILFYGTLMSLGVLF
                     VSLAVLRTSPGSPEKAVAMGFHAMVLSQLFFVFNVRNHSLFRKPGQIFSNPMLILSVL
                     VSIGVQVFITHIAIFQKVLSIHPLQLPEWGLILAGALLPTAVAQIHKFALGR"
     gene            15722..18175
                     /locus_tag="PLY83_01975"
     CDS             15722..18175
                     /locus_tag="PLY83_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV28837.1"
                     /translation="MPRPRNFELPLSKLRKKTPRSSLGFETTAEIGCLEGLIGQQRAV
                     EAMEFGLDVTSKGYNIFVVGDHGSGRTTYTLDQLRKRASQLPSPDDWLYVYNFMNSGE
                     PLAINLPPGMGKKLSESLSELVDELKTTLSKAFENSQYEDAKATLVKEFQEQVNALME
                     SVRNWALEKGFSIKRTPQGFVNIPLSLSKDEEGNVVSKEMQQEEFENLSEAEQKQLQA
                     RSEEVSQKTLEVLRKIRDLEKNLKEKIKNLEAEICRNAIRPYLADLKAQYGKGEKISE
                     WIDALTEDIIENFNIFIAAARDENAEVDFSRYSVNVFVSNDPEAGAPVVHETNSTYYN
                     LVGKVEYESRQGYLYTDFRKIVPGAVHRANGGYLVLDAEEILRQFMSWEALKRVLRTQ
                     ELSIENLGEQLGFVPVSSLRPEPIPIQIKVVIIGSRWIHYLLNLYDPEFQKLFKIKAD
                     FDVDMPRTKSTERQLACFVASYVQKEGALHFTADGVGEVIEFSSRLAGNQNRMTTHFN
                     QIEEVLVESVAWARKLSRKVVTADIVRKALNAKRFRANLIEERIQRAYENGQIRIDTD
                     GFAVGQINGLTVLDFREYSFGHPVRITANVYMGQEGVVNIEREVKMTGPIHNKGLLTL
                     SSFLGRRFAKDMPLSLSARLAFEQTYDGIEGDSASSTELYALLSALSGVGINQSIAVT
                     GSVDQFGNIQPIGGANEKIEGFFRYCSSRGLTGKQGVLLPKQNVINLMLDHEVLEAVS
                     KGLFHIWTVETVEQGIEILTGVPAGDANEKGEYPSGTVYGKVMEQLKTWMEKSRQLKK
                     SGSDSKKPEQESNEPENND"
     gene            18153..18851
                     /gene="nth"
                     /locus_tag="PLY83_01980"
     CDS             18153..18851
                     /gene="nth"
                     /locus_tag="PLY83_01980"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOV28838.1"
                     /translation="MNPRTTIEPETLLTVLDRLEKAWGKEKVPHERSFGSPLDGLIAT
                     LLSQNTNDKNSEKAFFEMKRRWPTWTQVMDVPVEALIDSIRVAGLAPTKAARIREILM
                     RIRLDFGSYDLDSLKNWDPVEARSYLENLPGIGPKTAACVLAFELGKPAFPVDTHVAR
                     IVRRLGWFTEKQSPAEIQEVLETAVPKERQGGGHLNFILHGRNTCHSRKPACHRCVLS
                     DLCPSATGKGLSRS"
     gene            18848..19996
                     /locus_tag="PLY83_01985"
     CDS             18848..19996
                     /locus_tag="PLY83_01985"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOV28839.1"
                     /translation="MSFLYRVGVCACRTYDPEEVRQAVAIAMERSGGGPRIGRNVLFK
                     ANLLAPAKPEQAVTTHPEILSALAAWVKGKNPVTNLSVSDSPGYLFAGQWPLFEGNCG
                     LRALKEKWGFDISPLNSKGFAETRTSFSATLGRFRIPIKAAEADTLFNVAKCKTHVET
                     EITACLKNTFGYLDTPTRKRAHRSGSIGKLCEAILDAHLSRIPDWNVIDAVVGMEGNG
                     PSHGNPKPLGWVIASENALAADVVAAWMMGYRNPFSIPLLAAAARRGIGPASRSHIDL
                     VGSTWENILSARFRKATSALRRFFPTPLRGLAHTLVKQTPFWDPSCCIFCGACEKVCP
                     AGAIRINCSTVLIDPEKCVRCLCCHEMCPVGAIGIRRNILARFLLRQK"
     gene            complement(20059..20355)
                     /locus_tag="PLY83_01990"
     CDS             complement(20059..20355)
                     /locus_tag="PLY83_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HOV28840.1"
                     /translation="MAEKWKDRLTDQLCKAILVLETEEEVYRFLEDIATIGEIRALAQ
                     RLEVARLLSEGYTYPQIAQQTGASTATISRVKKFLEYGADGYKIVLDRLKEKNQ"
     gene            complement(20476..21528)
                     /locus_tag="PLY83_01995"
     CDS             complement(20476..21528)
                     /locus_tag="PLY83_01995"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOV28841.1"
                     /translation="MYGRPHLAGFECKEDTTILLDKNENPFRLPPSLREQIRQVVSGI
                     DLNRYPDPEARALKQVLSEMTGISWREIVVGNGGDEILDLLFTAFTDKGQKVLTLSPT
                     FTQYPELCRRHKVEWVPVPLRFEENRIVFDQERFLETVSSCSPALVLLDTPNNPTGIR
                     IPNDFLSEFSERFKGVLVIDEAYGEFARETFLECFRNRTLPERLCILKTLSKAWGMAG
                     LRIGYAACSPSVAERVESVRDPYNVGLLPQEIAKIVLKYGEWMESRVLSIRYLRDQLV
                     KGINRITGWRAFESESNFVLVRSPIPISRVRERMEEACIRLKYLTLEQPGDWFRVSVG
                     REEELGMFMNLLQELN"
     gene            21694..21780
                     /locus_tag="PLY83_02000"
     tRNA            21694..21780
                     /locus_tag="PLY83_02000"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21728..21730,aa:Leu,seq:taa)
     gene            21793..21869
                     /locus_tag="PLY83_02005"
     tRNA            21793..21869
                     /locus_tag="PLY83_02005"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21827..21829,aa:Met,seq:cat)
     gene            21872..21948
                     /locus_tag="PLY83_02010"
     tRNA            21872..21948
                     /locus_tag="PLY83_02010"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21906..21908,aa:Met,seq:cat)
     gene            22029..23378
                     /gene="tilS"
                     /locus_tag="PLY83_02015"
     CDS             22029..23378
                     /gene="tilS"
                     /locus_tag="PLY83_02015"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOV28842.1"
                     /translation="MEHPVIQNARAAGKDQGWIESTEPLVAAVSGGGDSVALFFVLRE
                     IYRGPIIVAHLDHGIRRVSSRADAEFVLQLCKENGIDCVLRSACIPLQKERGESLEEA
                     ARRIRYAFLEEVRRKVRAAWIAIGHNSDDTVETILLNLLRGTGLAGLCGLPERRGSII
                     RPLIHSTRCELRQFLRERGFSWREDESNLDSQYLRNRLRNELIPLLERDYNPAVARRL
                     LSMRKTLIPCRETLEERGRNAAAFLRRELPCVVSSWDLSAVRRLDPSTCAEVFRSEAA
                     RLGLKTLDCDRFSCLMGLIHSSQGWRFQWEKSLEIRAGSGYLALLDRKMFDRPPEEPL
                     VLEEPSGTARWGLGTFRWERTEKGKLGFSDFSAFLPALPEKPVIRSSIDAVRYRGIET
                     MPWFYRKGWPTVTIGDRMSWTPFWSKACNFFEIETNSPRVRIQFYPEAGAGELGNGV"
     gene            23368..23934
                     /gene="hpt"
                     /locus_tag="PLY83_02020"
     CDS             23368..23934
                     /gene="hpt"
                     /locus_tag="PLY83_02020"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOV28843.1"
                     /translation="MEYELGRILLDGRTISARVAELAAEVSETYASKEFVAVGVLKGA
                     IFFLADLVRSLPRNSLVRLDFMAVSSYGASTKSSGIVKIVKDLDTDIRGRDVLLVEDI
                     LDTGLTLSYLLDLLKGRSPRSLRTCVLLDKPDRRKIDVRVDFRGFRIPDEFVVGYGLD
                     YSGYWRNLPDIHVVNPIEAPAGNIQPKE"
     gene            23942..25870
                     /gene="ftsH"
                     /locus_tag="PLY83_02025"
     CDS             23942..25870
                     /gene="ftsH"
                     /locus_tag="PLY83_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOV28844.1"
                     /translation="MNRIAKNLGLYLILIVLVVSLVNAFLTPSQNSQESTSLSYSDFI
                     KSAQSGQIEKVTIEENLVKATSKDGKHFKTYVLDPGPLAQDLVKLGVAVEFVPPQKAP
                     WWAGMMTSLFPTLLLIGAWIFILYNMQGGGSKVMNFAKSKAKLFLDNRPKVTFKDVAG
                     CDEAKEELQEVIEYLRDPERFARLGANVPKGVLLLGPPGTGKTLLARASAGEADVPFF
                     SISGSDFVEMFVGVGAARVRDLFEQARKMQPCIVFIDELDAVGRQRGAGLGGGHDERE
                     QTLNQMLVEMDGFDATTGIILIAATNRPDILDPALLRPGRFDRHIVVDKPDWKGRQAI
                     LEVHSKGKKLEEDVRLDIIARRTTGFVGADLANLVNEAALLAARRGRKALAMTDFEEA
                     IDRVIAGPERRSRLINAKEKEIIAYHESGHALIARLIPGCDPVHKISIIPRGHMALGY
                     TLQLPEEDRFLISRKELIDRICVLLGGRVAEEITQGDVTTGAQNDLERATQLARQMVT
                     EFGMSEKLGPMTLGRKQHEVFLGRDIVDDRNYSDEVAFAIDQEMRSIIDGCHSRVKTL
                     LSENLDLLHRVTRNLLDREILEGEELETLIAGGDLPPLSESGGTASKPATGVREEAAE
                     GTEPLPGGRGNPEPEPAA"
     gene            25953..27992
                     /gene="uvrB"
                     /locus_tag="PLY83_02030"
     CDS             25953..27992
                     /gene="uvrB"
                     /locus_tag="PLY83_02030"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOV28845.1"
                     /translation="MGIFKLNCPWPPAGDQPQAIEALSQGLAKGFPMQTLVGVTGSGK
                     TYTVANVIARFDRPVLVLAHNKTLAAQLYTEFKTFFPENEVHYFVSYYDYYQPEAYVP
                     AHDLYIEKDASINERIERLRLATTKALIERRDVIVVASVSCIYGLGKKKAYEEAVFGF
                     KVGEQWTRRDFFQSLLGNYYQRNDSVLEPAKFRVRGDVVEVFPAYSDTALRIFFDDRS
                     IDRIDEIEPVSGKVLRRKEQALIFPAQHYVTTKENIARALQAIESELEEQLARMTAEG
                     RFLEAQRLRERTRYDMEMLAQVGYCSGIENYSRHLEGREPGEPPGTLLDFFPPDFIMV
                     IDESHITIPQIRGMYNGDRSRKETLVRYGFRLPSSLDNRPLKWEEFRGYMKQVIFVSA
                     TPGDWETNHSSQVVEQLIRPTGVVDPEVVVVEAKNQVDDLIGRLSALDRNRERALVTT
                     LTKRSAEDLAEYLSEAGLKVRYIHSELDTFERADLLKHLRLGEIQVLVGVNLLREGLD
                     LPEVSLVAILDADREGFLRSHRSLIQMIGRAARNIAGTVVLYADTMNDSIRKAVEETQ
                     RRREIQRTYNESRGITPRTILKEVVGILPGGVEEKEGETAGFSETESFCSEDRSILEE
                     KMWKAVERLDFEEAARIRDLLASGGKGDLKGGPSHRRSGSKGTQSEKHFRRTSKR"
     gene            27916..30813
                     /gene="uvrA"
                     /locus_tag="PLY83_02035"
     CDS             27916..30813
                     /gene="uvrA"
                     /locus_tag="PLY83_02035"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOV28846.1"
                     /translation="MDHRIVVRGAREHNLKNISVELPKDKLVVFTGPSGSGKSSLAFD
                     TIFAEGQRRYVQSLSAYARQFLGVLNKPDVDDISGLSPSVSIEQKGISHNPRSTVGTV
                     TEIYDYLRLLFSRIGTPHCPLCGERVHRYSIDEILSVILRDFSGKRVEILAPLVRGKK
                     GEHRNVFEEARREGFLRVRVDGRVLWLEEEIALDRNRKHSIEVVVDRLTVSSENRDRL
                     SESTETALRIGTGSVLFATPEGEEKLLSENFACPKCEISLPEIEPRLFSFNSPFGACQ
                     ECSGLGSHEHFSVELAVDPERSVWDGALLPWKTTHYMLRKLKAVCEAKGWDLSKPFRG
                     LPPEIRDSILNGVSDRLPMTYRENGQVQTYLGRYEGLLPWLETRWAETESDAVTEELI
                     SFRTEDRCQACQGRRLRPEALAVEIDGLSIADVTETPVSSLLQRLDQLNLSPQALEIS
                     GQILDELKKRLSFMDDVGVGYLTLSRRADSLSGGESQRIRLATQIGSKLCGVLYVLDE
                     PTIGLHPRDTERLLQTLRAVRDLGNTVLVVEHDRDTMLAADYILELGPRAGEHGGQVV
                     AAGTREEIASLDCLTGPYLEGRVSGAIIRPTPREPGGWLTVLGAREHNLKDLDVALPL
                     GVMTCISGVSGSGKSTFMHEVLYKGLKRILDRDFRGRPGAHREILGWEALRNVVLVTQ
                     DPIGRTPRSNPATYTGLFTLIRELFSQLPEARIRGFQPGRFSFNVRGGRCEACKGEGE
                     SRISMLFMPDVFVPCEICKGKRYNHETLEVRYRGKNIADVLDLPVDEALQFFADIPRI
                     ASKLKVIEEAGLGYIRLGQPAPTLSGGEAQRVKLANELSKRFTGSTLYLLDEPTTGLH
                     YTDVRKLLLLLHKLVDQGNTVVLIEHNLDVLASCDYLVDLGPEGGEEGGRLVTTGTPQ
                     EVARSGIGHTSSCLRDFLGQLRIKDKEEGPCLKKEAKTRRKLSGGATPSSP"
     gene            30747..31523
                     /gene="rlmB"
                     /locus_tag="PLY83_02040"
     CDS             30747..31523
                     /gene="rlmB"
                     /locus_tag="PLY83_02040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOV28847.1"
                     /translation="MPEERSQNPQEIVWGRNSVLALIEGAPARIKKVLVARGTHHQSI
                     RKIEDSCHDRRIPLQRVDRITIERACPGNAHQGVVALISPADLHDVSELVHFVPENPE
                     PALVLLLDHLQDPGNLGSILRSAEAFGASAVVIPKRRAALPTGTVLKASAGAAERIPI
                     VSTPNLLCALDSLEPEGFWSVAMDPGAGEPLDGKPLPARMALVVGSEGQGISRPVENH
                     CDERRSIPMGGASGSLNASVAAAIAMYVWKRMIDKAGGAP"
     gene            31539..31613
                     /locus_tag="PLY83_02045"
     tRNA            31539..31613
                     /locus_tag="PLY83_02045"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31571..31573,aa:Val,seq:gac)
     gene            31709..32089
                     /locus_tag="PLY83_02050"
     CDS             31709..32089
                     /locus_tag="PLY83_02050"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOV28848.1"
                     /translation="MIIGVGVDLCSVSRIAAAIANRRFVEKVFSPEEQLYAESGGNPA
                     LHYASSFAAKEAFAKAGGWGLARTGLASVWVSRKSGRPEIRLSDRAKRLFPDRTGRIH
                     LSLSHEGDLVVAVVILEGINDPLL"
     gene            32073..33650
                     /locus_tag="PLY83_02055"
     CDS             32073..33650
                     /locus_tag="PLY83_02055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOV28849.1"
                     /translation="MIPFFDANTIREADRLAEEQFNFPPPLLMENAGKGAAEAILELY
                     GKRDWTILCGPGNNGGDGFVAARHLALAGCEVTVLLTEDRARIKSLPAANLKLLDQMG
                     CPMLQVAEHSDEFLAERLRKSAGIIDALLGTGSSGTLRGEPLRLLRLVPENGPVKVAL
                     DVPTGVDASTGIADPEAFRADLTLTFLAPKIGLRVMPGALCAGKIRVIDLGIEKKLLL
                     PPPSVEGYGIEDAARDWPSVSFSDHKGKKGTVLILGGSSLYRGAPILAARAALRAGAG
                     LVVLVVPECVARSASSALPEALVLSASKEDGGHVDFDSAVQILSNWSEKAGCLIFGPG
                     SGRSTTTGLLLEWISASWQKPLLLDADSLFFLQGRVSASSCLITPHEGEAGRLLRKPT
                     AEVANRRLESASILAARFGTVLLKGPYSICCDQKRIGIVLESTPSLAVPGSGDVLSGI
                     AGTLLAKGLSPWRAGLAASWAHARAGKLLSDSRGTETGLLAGEIADSLHLVFRELAVF
                     SKNGRSSVNAELCQPGI"
     gene            33622..34140
                     /gene="tsaE"
                     /locus_tag="PLY83_02060"
     CDS             33622..34140
                     /gene="tsaE"
                     /locus_tag="PLY83_02060"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOV28850.1"
                     /translation="MPNSASPEFELVFPSSGERETSSFGNLLSRFVFPGLLILLHGDL
                     GAGKTVLVRGITEGLSAGNVRSPSFTLVNEYPAAIPVTHADLYRRPPGGQEDLALEEA
                     LEEGRLVLVEWAENWKHPPIEEAWICRIELPEPPDSTDRRTIRIFSFGEKATFSLREA
                     ATSILKKGEELD"
     gene            34137..34802
                     /gene="tsaB"
                     /locus_tag="PLY83_02065"
     CDS             34137..34802
                     /gene="tsaB"
                     /locus_tag="PLY83_02065"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOV28851.1"
                     /translation="MTLNLAINCSTTWTSIGLSENGTVLAEMNLNIGRGQSSELPKAV
                     EEILRHSGRSLAQVDLFVLTIGPGSFTGVRVGLSYGLALAAARRCQVVPVNTLELMAY
                     QAAAVFRGNVLIPILNAKCGFFYAAAYGFNQETKRLSGFWEPGFYSFSQLDERLSSWE
                     GKALLILEKTKSFPLFSNPGFVYEDYIPSGGRLALLGDLFFERAVAPESVKGVYLRPP
                     DLG"
BASE COUNT         7871 a         9821 c        10043 g         7280 t
ORIGIN      
        1 ctcgttacgc cgatcgaaga catgaaggcc gtttttgatt ggaaggacaa ggtcccctct
       61 ttttaccagc cccctaggca ggagccggaa cccgtttcag ccgatgtgac ctcggcagag
      121 gttatcccga acgaggggtc acccgaagaa aactcctgcg tttccgagga ctgccttgaa
      181 gtcccccctc cgatgaacgt ccttctcgcc gatgggatga gccgtcgggc cgtcgaatcg
      241 ctgtcccggg aaattcggca gaacatcttt gccgttggcg ggggaaaaga cgatacggtc
      301 cctccggtca aaaaggtatc ggggctgaaa cctggggaag ccgtcggggt gatgttggca
      361 tggggagacg tcaacatcgg ggcgacaggg acgctgaccg ccctttccaa cgacggcagg
      421 ttcctcgctt tcgcccatcc tttcctgaat cacggagcgg tcgcctaccc tcttacggaa
      481 gcctgggtgc acgaagtagt tcccagcatc gaatcccctt tcaaactggg atcacctctg
      541 agcatcatcg gaaccgtgac gcaggacagg ccacaggcca tcggaggaaa gctcaatgtt
      601 taccctccct cctatgattt caccgtccgg ttcgacgatg tggataacaa gtcggtgcaa
      661 acgaagcgct ttcatgtggc cctagcaccc tttctcctca gcaagcttgc gccggaagcc
      721 ctaaccgggc tcctggacga cctttgggga agggtcggtg ccggaacagc ccggctcaat
      781 ctgtcggtaa cgggacccaa tctgtcgggt gggtggagca gaaataatct tttcttctcc
      841 gacaaggatc tgacaaagga agttctgaaa gaactgaaag aattgatgga aacgttcgcg
      901 ctgaacccct tcacggaaat tgcccccctg gggttccgcc tggatctgga gatgacctcg
      961 agcccgcgca tcctcctcat tgaggacgtg aaactggaca agaaaaagat taaccccggc
     1021 gaaaagttga aggtcgaagt ccggctgaga ccctacagaa aggatccatt cacgcgcacc
     1081 ttcgagctga cggtgccaaa ggatgcatcc ggccgcagca tggttgtcgt ccggggaggg
     1141 ggaatcaacg agccggggca ggaatcgatc atggaaggca agacaaccat tcgggatttc
     1201 cccactttgc tgaaggaact ttccgtccgg gaaaccaata accaggtggt cgtggaaatc
     1261 ctcgcttcag gagccccggg cgggaaggct cctatccgga aggggaaaga agcggaaccg
     1321 gccgatgaaa caccggaact tctgagcgaa atcactgaga aaaagctgaa agagggttcg
     1381 atgaagatct acaacacaaa ttactacgtg gacggactgc agcaaagaga aatttccgtg
     1441 ggaaccgata gcgcggagga ctaggcttgc aatggaaatc ttccgctggc ttggaaggat
     1501 tgcaatcggc ttttttgaaa cgcttggttt tttttccctt ttcctcctga aatcagcaag
     1561 gggcgtcttc cggggaaaat ggagccgttc ggatctgtcc aaacagctgg aacgtgttgg
     1621 cgtgagctcg atttttgtcg tgtcgatcac cagcgccttc acgggaatgg tcatggcggt
     1681 ccaaaccctt gaccaattcg ttcggttcgg agcgaccaac tacatcgggg gggttattgg
     1741 gctcagcatg gtccgtgaaa tgtccccggt cctgaccggt cttgtggtga ccggacgggt
     1801 tggagccgca atggctgccg agatcagctc gatgcggatc acagaacagc tggacgcctt
     1861 gcaggcattt gggttggacg aagtggcctt cgtcggttcc ccccggctcg ttgcgggcct
     1921 agtcatgctc cctctgctga cgatttattc ctttgtcatc ggaatcggcg gaggctatct
     1981 tttcgtggtc acccggggca tccatagcac aaccttcctg aaatcgctgg aagctctcct
     2041 tgagccttct gacatctttg gggggcttct taaagcggcg tttttcggcg tcatcatcgc
     2101 gacaacggcc tgcgcggagg gattccgtgc gggcaatgga gccatcggcg tcggcgaggc
     2161 cacgacgcgg gcggtggtct ggagcaatat gctgattctg gcgttcaact acatcctttc
     2221 aacgatcctt ttcggaggta tccggtgaag gcccggctgc tcgaagccac tgccatcgaa
     2281 aaatctttcg gggaaaacgt cgtcctcagg ggaatcgatc tggctctctc ggccggagaa
     2341 accctttcct tgatgggtcc atcgggatgc ggaaagacga ccgtgctccg gatggtcgtg
     2401 gggctgatca agcctgatcg cggaaaaatc accctcttcg gcaaagagat gaccgattcg
     2461 gaggacgatg aatctgtcat cgagctgaag cggaggatcg gcatcgtttt ccaggggggg
     2521 gctcttttcg actccctgaa cgtgggagaa aatgttgctt tccccttacg ctactgtctt
     2581 ggcctctcgg acgaagatga gatccaaagg cgcgttttcg aagtgctaga gcaggttgaa
     2641 ttgcccggaa ccgagtcgct gatgccggcc gaactttcag gaggcatgaa aaaaaggatc
     2701 gccatcgcgc gagccctcgt ccacgagcct gacatccttt tgctcgatga accgacaaca
     2761 gggttggatc cccagacagc ccgccacatc gaccgtctcg tttcagaaat cggaaagcgg
     2821 cggaatctcg ggatcctcgc agtaacccat gatctggtca ccgctatcgg gatttccgat
     2881 acaattctcc tgatggacca ggggataatc tcctggaggg ggactcccga agaatggaaa
     2941 acctctgagg atccgaatgt caggaggttc gcaactgggc ttcgccctgt ccgggcagga
     3001 aggacggtgt gtccatgaac agggagttca aggtcggcct gacggtcttt ctcgcccttc
     3061 tagcccttgg tggaatggtc ttcatcacgg gcggggcctt attcaggcat ggcggcaatt
     3121 cctacgagat cctattccgg gacgcaatgg ggttggagca gggtgccccc gcctatgttt
     3181 ccggcatcga gtcgggtgct gtccgatctt tgaggctatc gcccgaagga gtgatcgtca
     3241 ccgtgatctt ggatccttcg gtgaggattc ccaaggacag caaaatcctg atcggtacgg
     3301 gggggctttt aggaaagccg cttcttcgaa tcgagcgggg atcgagccag gagttttttc
     3361 agcccggcga tcggctttcg ggggaaattc cccccgattt cgattccgtc ctcgaagaaa
     3421 ttcagaaaaa cctgaaagca ctcggggcaa cctttcaaaa cctcaacgag gttctcggtg
     3481 atcccgaacg gaaagccaag ctggggaagg cgctcgacaa cctgcccgat ctcgtggagg
     3541 acagccgttc tgcaatgcgc cgaatcgggg cggcgggaac ggaaatccag gaactcggac
     3601 gattggcgcg caagcagatc aaactgcttt ccgaccgtct ccaggaactg ggggggcacc
     3661 tggatcgggt tgttctcgaa aacagggaag atgtccgaaa atccattgtt tctctggcct
     3721 ccctcttgaa gagggcggat gaaacgtttg cccagtttga cgaagaccgc atggctggaa
     3781 aggaaatgcg tgaagccatc cggaaccttt caaaagctgc caagggtatc gaagcccttt
     3841 cctcaagcct ttccgaggct ctcggagatt cccttccgac ttccgaggac aaggaaggcc
     3901 ggataaacct gaaagaaacc cttcaggggg ccgcccggat cgtttcctct gtcggatcga
     3961 tccgcacatc gggtgaggtt gccctgcatc gttctgtttc aggggaggac gacaacgagg
     4021 gatgggcgat ggacctctcg ctccaaatcg gaaaggaaca ctctctgtgg aacctgcttt
     4081 tagaaggcac cggactgggc aacggaagcc gggttaccgc ttccgcgctt tatcaaaccg
     4141 cctggggacg cgtctggggg ggactcgtgc ggggagatgt aggattcgga gccgccgtgg
     4201 atttccgctc tgaatttccc ttttctcttt cggcggagtg gtgggatgaa caggggggga
     4261 gctgggcctt tcggggaaaa tggtacgtca agccgcgctg ggggttctat tacgagcggc
     4321 tggagcccgg gcatgacccc gccagtgatt tcgttggtgt tttttaccag ttctcgcgat
     4381 aggtagggaa aagctttgtt catcacattt gaagggatcg acggctgcgg gaaatccact
     4441 caggcacgac ggctggttga gttcctgcta agggaaaagg gaaacgggaa agccctctgg
     4501 acccgagaac ctggcggatg gcccggttcc ggggatctac gggaattgat cctttctcag
     4561 gagttcgagc atccatggac cgaagttttc ctttttcttg ccgatcgctg cgagcatgca
     4621 aaaaaaatca ttgaacccgc ccttcaggaa ggggtcatcg tgatttgcga gcgattcgat
     4681 gattctacca gagcctacca ggtgtggggg cgaggcattg aactcgagaa aatcgaacgg
     4741 gcactggcct tggcagaata cccgcggccg gatcgaacga ttctgctgga tgtctccgtg
     4801 gaaacggcca tggggcgagt cttggccaga gcaaacctcg accgatacga aaaggagggg
     4861 aaaagcttcc tcgaacgcgt tatcgaggga taccggttcc ttgctgcgag ggaaaagagc
     4921 agaattcgcc ccgtcaacgg agaaggaaaa ccggatgagg tcttcgccag gatccttgac
     4981 tcccttgagg ggtgtttctg atgactgaac atcctaccaa tcccgatcaa gggggagaaa
     5041 atgggatcag ccttttcgac gccgtggcgt cgagtagggc ctggaaggaa ctttcctcgg
     5101 ctctcgaagc gggagtcgtg ccccagtcgg ttgttttctg ttctccctcg ctgtttcacg
     5161 atcctttcgt gagtctttac gctcacaccc ttttttcagg gaacggatcc ggctggctcg
     5221 gggaaaccca tcccgacctg ctccgcctgg ggaccccaga ggctccgccg ggaatcgaaa
     5281 ccttccgtga acagctcgta gagctctatt caagccccgt ggccgctccc tacaggctag
     5341 cggtcatcca ttgtgccgac cgactcagcc ccccagcggc aaacagcctt ctaaaaatca
     5401 cggaagagcc accccccagg ggcaggatcc tcttcctcct ggaagaagaa aaccttcttc
     5461 cgaccctgcg gagccgaagc tggatcctga aaatgccccc cgaggaggtc ctggctcccg
     5521 cagctccccc aaagaacgac gaggaatggg ttcattggct ctcggaagct tcttcctgga
     5581 aaacggatgc cgttctcctg cgtgcgacag cctgggcaag gtggtatgct ctttcgggtc
     5641 aatttcggaa ggccgaggaa ctggatacct tggtggttct gtttcagaaa acaaagcttt
     5701 cggcttcaat ggcgattgat ctaatttttt tgatgacaag agaggaatcc acgtttgaag
     5761 actttcttga ttctttacgg taagccccgt taccttgggt tcttaaggac ggaggaggaa
     5821 cctgttcttc gtaaggggga tttgatcatt atcgaatccc cgagaggcag agaaatcgga
     5881 atcgttgcgg gccccatcag tcccagccag gaggagagat accgaaaagc gcccacgtcc
     5941 gaaaccgccg atggaagttc gaagcccgga accccgaccg acgaggtgca gttcatcggt
     6001 tttccccttc ccgacgagac cggattcaga aaggagtgcg ccgaggaaga aaaccaggtc
     6061 ctgcagcggg ctagggaaat tctgaagacg cacaatcttc ctatgaaact ggtggacgtc
     6121 gaatatctcc tcgaccgctc caagctcttt ttctatttca cggccgagca acggattgat
     6181 ttcaggggat ttgtccgaga tttggcgagg gagttcaaaa cccgaatcga acttcgccag
     6241 atcgggggga gagatgaagc gaaagttgcc gcgggaattt cacaatgcgg gatgccttgc
     6301 tgctgcagca catggcttaa ccgcttcgag cccatctgca ttaagatggt aaaacagcaa
     6361 aacttagccc ttaacccgac aaagatttcc ggaatttgtg gtcgcctgat gtgctgcatg
     6421 ggatacgagc atcaaaccta ccaggaactc tggaagggtc ttcccaaccc ggggacgaag
     6481 ctcaagtcac tgacgggaga aacctacctg atctcgggtg tcgacctgat ggggaaatcc
     6541 ctgcggatct tctgcccgga tcgaaccgaa ttgctggtcc cggtttccga attcgcaaaa
     6601 ttcaaggaag ccatccagaa aggcgaggtc tgggagcccc agcgcgagga ggttgtgccc
     6661 cttcccttcg attcgctcct tcaggaagct gttacggatt cggacaggat cgtcgaggac
     6721 gggatttgcc ccgttgaaaa gccagtccgc cagcgccccg gccggttgat ctccagggag
     6781 cgagccgttc gaagaaccgg ggaacgggaa acacgggaaa gagaacttcc tgcaaaacct
     6841 gttgtttcca ctgttccagc ggttaagagt gaggcgcctg ccccggaaga aacccggacc
     6901 aaaagaagac gaccgcgacg gaaaaagaga aagcccgccg ccgctgcacc ggagatggct
     6961 ccggcgaaac cggctctcga aacccggatc gaggcctcgc cgaccggccc cgatacccag
     7021 gaaaggcctc acaggaaacg gaattccagg cggcgtaaat cacgaaagcc ccaggcggag
     7081 gaatagccgt ttcagctgac tctcaacagg aggtggatac ccctgaattt ctttgaacgc
     7141 tttcgatccg gactgaaaga ggtcaggtcc aagtggggga actccctgac ccggctgata
     7201 tcggcctccg atgaaaaccc cgatttctgg gaggaaatcg agggaaccct cctcaagggt
     7261 gacgtggggg tcgacctttc ggaaaagctc atcgctgatt ttaggaagct gagaaaaaac
     7321 ccggggatgg accgatgccc tccggtttgg ctcttcggga aacagcttga ggcgattttc
     7381 gccgggaatc ccctgaacgg ggctcctctc cgcctgaatg gaaaaccttc ggtggttctc
     7441 cttgccgggg tcaacggcag cgggaaaacc accaccgcag gaaaactggc ctcgcagttc
     7501 acccgggcag gaaaccgcgt cgtgcttgcc gccgccgaca cttacagggc tgctgccatc
     7561 gaccagttga agacctgggg agaccgctgc ggcgcaagga caatcagcca cgggatggga
     7621 agcgatccag ccgccgtcgt tttcgatgcc atccggtccg cgaaagccga cggggcggaa
     7681 tgggtgattg ccgatactgc gggacggctg cacacacggc acaacctgat ggaggaactg
     7741 aaaaaggtgg cgcgcgtggt ggaacgagag gtcggtccgg gcaacgcgga atttttgttg
     7801 gtcatcgacg ccgtgatggg gcagaactcc tttcagcagg cgaaggtttt tggcgaagct
     7861 ctccccctca ccggcatcgt cctgacgaag tacgacaaca cggctaaggg cggggtgatc
     7921 ctgtccgtcg tccacgatct ggggctaccc gtgcgttatg tcgggctggg cgaaaaagtc
     7981 gaggatcttg ccctttttca gccgaaggaa tttatcgaag cccttctcgg cttgccagag
     8041 ggcgatttgt gcaaggaggg acttcgttca tgatcgagcg ctacgaaacc gaagaaatga
     8101 aaagaatctg gtccgatgaa aaccgctttc aaaaatggct cgaggtcgaa ttggcagttt
     8161 gcgaggcctg gacggaagaa ggcaaaatcc ctgccgaagc gatggacacc attcggaagc
     8221 gcgccggttt cgacatgcag agaatcgccg aaatcgaatc cagcactcac catgatgtca
     8281 ttgcttttgt cacttccgtc gccgagaaaa ttggacccga gggacgtttt attcacctcg
     8341 gcttaacgag cagcgacgtg gtggacactg cgggatccct tctgatcctg gaagcccttg
     8401 ccgttctgac cgaaacgctg aagagctttt ccggacttgt cctggaaaaa gccagagagt
     8461 accgccacat cccctgcgtg gggagaaccc acgggatcca cggtgaaccg acaactttcg
     8521 ggctgaagct cctaaactgg cattcacagc ttctgagaga cctcgagagg cttgagcttg
     8581 cgaacaagca catcagggcc ggaaagattt ccggtgcagt cggaacctat gcccactgcc
     8641 ccccgcaaat cgaggaaaaa gtctgttcaa agctgggatt gcagagggct gccgtttcaa
     8701 accagatcct tcagagggac cgccacgccg aggtcctgac ggccatcgca attctcggat
     8761 ccacgatgga acgaatcgca acggaaatca ggcatcttca gagaacggaa gtcctcgaag
     8821 cagcagaacc attctccaag gggcagaagg ggtcttccgc catgccccac aagagaaacc
     8881 ccattctttc cgaaagggtc tgcgggatgg cgcgccttct gaggggatac gcccttgcct
     8941 cgatggaaaa cattgccctt tggcatgaac gggatatcag tcattcgtcg gtggagcgcg
     9001 ttgcatggcc ggatgctttc cacctggctc actactcgct gaaaaccctg atgcgaatcc
     9061 tcgccggact gcaggtcgat cccggcagaa tggcgggaaa tctcgaaatg acccgggggc
     9121 ttcttttcag ccagagagtc cttcttttcc tgatcgagga aatgggagtc agccgcgagg
     9181 aagcctacgc catcgtgcag gaaaacgcta tgcggtgctg ggcgggggaa ggccacctca
     9241 gggaccttct gtcctctgac ccgcgtgtga gccgttatct tgacgatgat gccctggacc
     9301 tgcttttcga ttaccgttac ttcacccgat atgtcgacga aattttctcc cgttttgaaa
     9361 acaagggaga aaccgaccca caaaacgctt gaggaggtgc tcgcaatgtc gcttccggaa
     9421 agaaatctgg cgctggaact tgtccgggta acggagtctg ccgccatggc tgccggccgg
     9481 tggatgggcc ggggagacaa gaatggggct gacggggcag cggtcaatgc catgcggtac
     9541 atgctgaaca cgatccggat ggatggaacg gttgtcatcg gagaagggga aaaggacgaa
     9601 gccccgatgc tctttaacgg cgaacgcctc gggactgggg atgagcccaa agtcgacatc
     9661 gcggtggatc cgatcgacgg gacgaggctc ctttccctcg ggaggcaaaa ctccatcagc
     9721 gtcgtcgcgt tggcggaacg agggagcatg tatttccccc agcacatttt ttacatgaac
     9781 aagctcgcga cggggccgga agcagcggga tcaatcgata tccggctacc gatcggggag
     9841 aacatccgca gggttgcaaa ggccaagaaa aagaacgtag aggacgtcac cgttgtcttg
     9901 ctcgaccgcc cgcggcatga aaagctcatc gcggacatcc gcgaagcggg cgcaagaatc
     9961 cggctaatca ctgacgggga cattgctggg gcactgatga catgcaagga ggatgccggc
    10021 gttgatcttc tcgccggtat cggcggatct ccggaggcgg tcattaccgc ctgcgccctc
    10081 aagtgcgtag gaggcgccat gtactgcacg ctctggccca gaaacgagga agaggcggag
    10141 aggtgccgag aactgggcat ggatcttgag caagtcctca gcatggacga tctcgtgaaa
    10201 agcaataacg tcttttttgc cgcgaccggc atcacgaacg gcgatttgct tcagggcgtg
    10261 cgttacgaag gagaccgcat ccgaactcaa tccatcgtca tgcgttccgc gagcgggacg
    10321 atccggtaca tcgaggcgat ccataagctt tcaaagctgg aaaccatcag cggcatcaat
    10381 tactagcgcg gcagggatgg gcaaaattcc gccatattga cgcgccgttt ctgcgatgat
    10441 atatttacca tgttctttta acctgcaatt gcaggaaaac gaaaaaccgg aggggtacag
    10501 tcaatgaaag gaacagtcaa gtggttcaac gcaacgaagg gatacggttt cattaccacg
    10561 gaagagggga aggacgtctt tgcccattac agcgccatcg agatggaggg cttcaagact
    10621 ctggaggaag gccaggccgt tgaattcgag gttgtagaag gagccaaggg accccaggca
    10681 gcccatatcc gcccgctcta ggcgcggctc gaatagagtg gggcgccggt cttaagccgg
    10741 cgcccctttt tttatttccc ccatttccag tatcctgcca ctgcctgccc taccgaaacg
    10801 cattcatcgt ttggcgagag gagcgtatga atgaccggga tcagtcctct ccttcgggct
    10861 tccgcgatca tgagtgaaaa aaggcgccgg ttctgccaaa ccccgccgga gagaacgaca
    10921 cgccgggttc ctttccgttc cgcggccaac gcggcggcct cgcccagggc ccgcgcgagc
    10981 cccccatgaa aggcggctga aatcgaagcg ggcgactttc gccctctgtc cgcgaaaagt
    11041 ttccggattg cgggccgcca gtcaatcagg atttttccgt cggattcaac cacggaaaag
    11101 ggaagatttc ccgaacctct cgcggcagcc tccaggacca tgggagcctg tccgtcaaaa
    11161 gttaccgatt ccacgaaccc cagaagggca gacaccccgt caaacagcct tccgcaagaa
    11221 gtcgttttca cccccgcttg gattgaaacc tctaggaacc ggtcaaaatc cggtgaaagc
    11281 ttgctttttc gttttgccca ctctcttgcc tcttcattga gtccgctggc ataggcaagc
    11341 gacattccgc aacgccaggg ctcccagatc gccttttccc cgcccggaag agtgaatggg
    11401 accagatgtc cgagacgctc gaaaccggaa gcatcccccc agagaatttc ccctccccag
    11461 atcccgccgt ccgtcccgta ccccgtccca tcgagaatga gaccaacggc cggctcatcg
    11521 atcccatttt caaaaaggca ggctgcgagg tgggcatggt ggtgctggac ggcaagggtt
    11581 tcggtagggt taggaagcac ttcatggcag aggcgggtag acaggtacag ggggtgcagg
    11641 tcgtgaacca ggaattccgg atggatggag tagagggaaa gaaagtgctt cagggcctcc
    11701 cggtaaaggg tcgctccccc gatcgatttc aggtcgccga ggtactggct tggaaagagg
    11761 gcgccttcat gggtgatgga gaaggtcgcc ttcatttcgc ctccggaagc cagaatggaa
    11821 ggcccgcttt tcggtagaaa aaggggctga gggaccacgc cccgagatct ccggacgtag
    11881 aaggcccggc tcccagctac ggaaacgacg gaatcatcga tgcgcatgca gatctttcgg
    11941 ttatgcagca agaaggcatc caccagggac gacaggcttc ccagggcctc ttcgttgtcg
    12001 gccacgatgg gttcctcgct tagattcgca ctcgtcatga ccaggacctc aagcttttca
    12061 agaagcaggt ggtggagggg agtgtaggga agcatgatcc cgatcgaatc cagtcctggg
    12121 tttaacagag gggaaaggcc cgagtcggac cgggcctggc acaggacgat cggccgccgg
    12181 gaagacatca ggcagtcctt ccccttctcg gtcaaaacgg ccaccttaag ggcagcctcc
    12241 agatcccgga ccatgatcgc aaatgggcgg tgggaacgcc gtttccgatt ccggagttcc
    12301 ccgaccgcgt catcgcttcg cgcgtcacag gccaggtgaa atccacctag tcctttgact
    12361 gcaacgatgc tgcccctgga aatcagttct gtcgcggtct ggattgcagc ctcgtcttcc
    12421 gccagggtga tcccccggca gtcacaggcc cataagcggg gaccgcagac caggcatgca
    12481 tttggctggg cgtgatatcg ccgattggta ggatcccggt actcggccga gcactcctcg
    12541 cacatcgaaa acttggacat tgtggtattc gaacgatcgt agggcaggtc ccggatgatc
    12601 gaaaacctcg gcccgcatag ggtgcagttg ataaagggat agcgatagcg gcgatccgaa
    12661 gggtccctca tttcttcaag gcacgattcg caggtggcga tatcgggcgg gatcagcacc
    12721 cgccggacag ctcttccctc gcttttcagg atttcaaagg agtcgggagg agaggaagcg
    12781 ttgtcttgcc tttctaaagg aatcaccgat tggatatagg ccgcggtagg acactcccgc
    12841 tcgaggcgtc tcaggtagcg gtcgatttgg ccgcgttcac cccagagctc cagctcaact
    12901 cccgaactag tgttcctgac cgaccccttg agcttttcct ccatcgccag ccgggcgcag
    12961 aaaggacgga accccactcc ctggaccaca ccgccgatca ggaaacgtgc tttttccatg
    13021 gttgacaacc ctccgtactg ggcgcatttt aagagaaaca cggcatcacc gcaaggcaca
    13081 tcggacacga ggaggctcag cctgatgtca ccggcaaccg atgatcatcc gcatagcgtc
    13141 cccgttgaag atctgttgcg aagtctttcc tcggacccag agagggggct gtcccaggag
    13201 caggcacaag ccgctctcaa acggtacggc cccaacgcat tcgccaaaat caaacccagg
    13261 ccctggtggt cgtttctcct gtcgcagttc aagagcccaa tgatcggatt gcttgccctg
    13321 gctgccgtgg tcagtctcac gatgggagaa atcctggatg cggccgccat tttcaccgtg
    13381 atcctgctga atgcgttgat cggtttcttt acggaatttc gagccgaaaa agcgatggaa
    13441 gccctccggt cgatgacctc gccgaacgcc cgcgtggtcc gcaacgggag cgtagccgtc
    13501 gtttccgcgg aaaaagtcgt tccgggagat cttcttctcc tcgaggcggg agacatcgtc
    13561 ccggcggatg cccgtcttct cgagtcgaga aacattgcat tggacgaggc gtcgctgacg
    13621 ggagaatcga tgccggtcga aaagaacgtc gactgcctgc cggctgaaac accgcttgcc
    13681 gaacggctga attgcgtttt ttcgggaacc gccgtggtcc ggggaaacgc gaaagccttg
    13741 gtcttcgcca cgggtaccga ttccgaactc ggcagaatca gcatgcttct ggaaagcgtg
    13801 aaaaagcagt caacccccct ggaggaacgg ctggaacagt tcagccgttt tttgatcgtc
    13861 ctggtcgggg ttcttgcgtt tctcgttttc gcactgggtg tctttcaggg cagagggctg
    13921 ttcaaaatgt ttgaaacggg aattgccctg gcggttgccg cggttcctga aggactcccc
    13981 ttcgtggcga ccatgacgct ggcgattggt gttcacagaa tggcaaaaca gaaggctctc
    14041 gtcagaaacc tttcttccgt tgaaactctc ggttccacta cggtgatctg tacagacaaa
    14101 accggaacct tgacgctcaa cgacatgacg gtacgactcg ttctccctgt ctgcgacgaa
    14161 caggaaagaa cccttctaca gacggcggtg ctttgcaaca acgcgaagct ggagcaaagc
    14221 ggcgatatcg gcgatcccat ggaaatcgcc ttgcttcgtg ccgcaaggga gaagggaatg
    14281 aacctcgaag aagtgaaaat gcgctttcct cgcaccgggg agaacccctt cgattccagg
    14341 acgatgacca tggaaacttt tcacggagtt tggaaagcct tgaagggggc accggagaag
    14401 gtcctttccc aaacgaacca catttggacc gaagaaggca tccgacccat ggggggacaa
    14461 gaccggaaac actggctcga gcgggcggaa gagatttccg ggcaaggaat gcgctccctg
    14521 gcgttcgcct ggggaaacga aaaagagggg ctggccttcg tcggcctcat cggaatcatc
    14581 gatccgccgc ggccggaagt caaggacgcg gtttcagcct gccatgaagc cgggatccgt
    14641 gttgtgatgg tgaccggaga tcaccttgcc accgcccgct cgatcgcgaa agaggttggc
    14701 atcctctgcc cgctgcaccc ggacagcctc gaggggaagg aattggaatt gctggatgaa
    14761 aaaagcctct cttcaaaaat agcggcagtc tcggttatcg ccagggtggc tcccgaacac
    14821 aagctgaaga ttgtccaggc cttccaggca gccggagaaa ccgtagccat gactggagac
    14881 ggcgtcaacg atgccgtcgc gctgaaacag gcggatgtgg gaattgccat gggcatccag
    14941 ggcaccgagg tcagcaaaga gtcttctgac ctgatcctgc aagatgaccg cttctcgacc
    15001 atcgtaaatg ccatcgacga gggaaggcgg attttcggga acatccggaa atccgttcta
    15061 tttctccttt gctgcaacct gagcgaggtc tttaccgtcc ttttcgccgt tctttcccga
    15121 ttgccggcca tcctgctccc gttgcagatt ctctggatca atctcattac ggatgtcctt
    15181 cccgcccttt ccctggccct ggaccccgcc gaacctggca caatgcggcg tccgccgatg
    15241 ccccggtcgg aacgcattct cactccacgg catctccggc tgatcctctt ttacggaacc
    15301 ctcatgagcc tcggggttct attcgtctcc ctagcggttc tgagaaccag ccctggaagt
    15361 ccggaaaagg ccgtcgcgat gggattccac gccatggtcc tctcccagct cttcttcgtt
    15421 ttcaatgtcc gaaaccacag cctctttcgc aagccgggac agattttcag caaccccatg
    15481 ctgattctct ccgtgctggt ttcgatcggg gtccaggtct tcattaccca catcgccatc
    15541 tttcagaagg tcctttccat ccatcccctt caactcccgg aatgggggct gattctcgca
    15601 ggagctcttc tgccgacggc agttgcccag attcacaagt ttgccctcgg gcgctgaagg
    15661 catccgggcc ctgcctgtgc taacataaac ccatggaccg cactcgactg gagggaaaga
    15721 aatgccgaga ccaaggaatt ttgagcttcc cctttcaaag ctgagaaaga aaacgccgcg
    15781 ctcttccctc ggattcgaga caaccgcaga aatcggctgc ctcgagggac tgatcggcca
    15841 gcagagagcc gttgaagcga tggaattcgg tctggatgta accagcaagg gatacaacat
    15901 ttttgtcgtt ggcgatcacg gaagcggacg aaccacctat acgctggatc agctgcgcaa
    15961 gcgtgcctcc caactccctt cacccgatga ctggctgtac gtctacaatt tcatgaactc
    16021 cggtgagccc ctggcgatca acctccctcc cggcatgggc aagaagctct ccgagagtct
    16081 ttccgaactc gtcgacgagc tgaagaccac attgagcaag gcttttgaaa acagccagta
    16141 cgaagacgcg aaggccactc tcgtcaagga gttccaggaa caggtcaatg ccctgatgga
    16201 atcagtccgt aactgggccc tggagaaggg attttcgatc aagcggaccc cgcagggatt
    16261 cgtgaacatt cccctttccc tgtccaagga cgaagagggg aatgtcgtca gcaaggaaat
    16321 gcagcaggaa gagttcgaaa acctttcaga agcggaacag aaacaacttc aggccagatc
    16381 ggaggaagtt tcccagaaga ccctcgaggt cctgagaaag atccgggacc tcgaaaagaa
    16441 cctcaaggaa aaaatcaaga accttgaggc ggagatttgc cgcaacgcga tccgccccta
    16501 cctggcggac ctgaaggccc agtacgggaa aggggagaaa atttccgagt ggatcgacgc
    16561 cctgacagaa gacattatcg aaaatttcaa cattttcatc gccgccgcac gcgatgaaaa
    16621 cgctgaagtc gatttcagcc gctacagcgt caatgttttt gtatcgaacg acccggaagc
    16681 aggggccccc gtcgttcatg aaaccaactc gacctattac aatctggtcg gcaaagtgga
    16741 atatgaaagc cgccagggat acctctacac ggattttcgt aaaatcgttc ccggtgccgt
    16801 tcaccgggcc aacgggggct acctggttct cgatgccgaa gaaatccttc ggcaattcat
    16861 gtcctgggag gctctcaagc gcgtcctccg tactcaggaa ctttccatcg aaaacctggg
    16921 tgaacagctt gggtttgtcc cggtttcatc cttgagaccg gagccgatcc ccattcagat
    16981 caaggtggtg atcatcggga gtcgctggat ccactacctt ctgaacctct atgatccgga
    17041 attccagaag ctgttcaaga tcaaggcgga ttttgatgtt gacatgccgc ggacaaaatc
    17101 gaccgaacgt cagcttgcct gtttcgttgc cagttatgtc caaaaagaag gcgcccttca
    17161 tttcaccgcg gacggcgtcg gtgaggtcat agaattttca tcacgcctgg cgggaaacca
    17221 gaaccggatg accacccatt tcaaccaaat cgaggaagtc cttgtcgaat ccgttgcatg
    17281 ggcgagaaag ttgtcacgga aagtcgtgac cgctgacatc gtaagaaagg ccctgaacgc
    17341 caagcgattc cgtgctaacc tgatcgaaga aagaatccag agggcatacg aaaacggcca
    17401 gattcgaatc gacaccgacg gttttgccgt cgggcagatc aacggactca ccgtcctcga
    17461 tttcagggaa tactctttcg ggcatcctgt tcgaatcaca gccaacgtct acatggggca
    17521 ggaaggcgtc gtcaacatcg agcgcgaagt caagatgacc ggcccgatcc acaacaaggg
    17581 gcttctgacc ctgagcagtt tcctgggacg gcggttcgcg aaagacatgc ctctttccct
    17641 ttctgcacgt ctcgctttcg agcagaccta cgacggaatc gagggggaca gtgcctcttc
    17701 cacagagctt tacgcgcttc tttccgccct ctcgggagtg gggatcaacc agtccatcgc
    17761 cgtaacggga tccgtcgacc agttcgggaa cattcagccc atcggcggag ccaatgaaaa
    17821 aattgaaggc ttcttccgtt actgctcatc ccggggactc accggcaagc agggggttct
    17881 gcttcccaag caaaatgtca tcaatctcat gctggatcac gaggtactgg aagccgtttc
    17941 aaagggattg ttccatatct ggacggtcga aacggtggag caggggatcg agatcctcac
    18001 aggggttcca gccggggatg cgaatgaaaa gggcgagtat ccctccggca ccgtctacgg
    18061 gaaagtgatg gaacaactca aaacatggat ggaaaaaagc cggcaactca aaaagagcgg
    18121 ttcggacagc aagaaaccgg aacaagaatc gaatgaaccc gagaacaacg attgaaccgg
    18181 aaacgcttct taccgtcctg gaccgtcttg aaaaggcctg ggggaaagag aaggtgcccc
    18241 acgaaagatc ttttggcagt cccctggatg gactcattgc gacccttctt tcgcaaaaca
    18301 cgaacgacaa aaacagcgag aaggcgtttt tcgagatgaa aaggcggtgg cccacctgga
    18361 cacaggtgat ggatgttccg gtggaggccc tgattgattc cattcgggtt gctggcctgg
    18421 ctccgacaaa ggccgcgaga attcgcgaga ttctcatgag gattcgcctc gatttcggaa
    18481 gctatgatct cgattccctc aaaaactggg atccagtcga ggcacgctcc tacctggaaa
    18541 accttcccgg aatcgggccg aaaaccgccg cctgtgttct cgctttcgag ctaggaaagc
    18601 ccgcttttcc cgtcgacacc cacgtggcgc gaatcgtccg aagattggga tggttcacgg
    18661 aaaagcaaag ccccgcagaa atccaggagg tgctggagac tgctgttccc aaggaaaggc
    18721 agggtggcgg gcatttgaac tttatccttc acggcaggaa tacctgccat tctaggaaac
    18781 cggcctgtca ccggtgcgtc ctttcggatc tctgcccttc ggcaacgggg aaaggtcttt
    18841 cccggtcttg agttttcttt atcgcgtcgg tgtctgtgcc tgtcgaactt acgatcccga
    18901 ggaagttcgg caggccgtgg caattgccat ggaacgttcg ggcggagggc ctcggatcgg
    18961 gaggaacgtc cttttcaagg ccaacctcct ggctccagca aaaccggaac aggcggtcac
    19021 cacccaccca gaaatccttt ctgcgctggc agcctgggtt aaggggaaaa atccggttac
    19081 aaatctctct gtatccgatt ctccggggta tctttttgcg gggcaatggc ccttattcga
    19141 aggaaattgt ggtcttcggg cgctcaagga aaaatggggc ttcgacatat ccccgctaaa
    19201 ttcgaagggt tttgcggaaa cccgaacgtc cttttccgca acgctcggaa ggttcaggat
    19261 ccccatcaag gcggccgaag ccgacacgct tttcaacgtg gccaaatgca aaacgcacgt
    19321 cgaaacagaa atcacggcgt gcctcaaaaa cacctttggt tatctcgata cccccacacg
    19381 aaaaagggcg catcgcagcg gatcgatcgg gaaactttgc gaggcgatcc tggacgccca
    19441 tctttctcga atccccgact ggaacgtgat cgacgcggtc gtcggcatgg aagggaatgg
    19501 gccttcccat ggaaatccaa agcctcttgg ctgggtgatc gcctcggaga acgccctggc
    19561 cgcggatgtt gttgccgcat ggatgatggg atacaggaat cccttctcga tccctctcct
    19621 tgctgctgca gcgcgcaggg ggatagggcc ggcatcgcgg agccatatcg atcttgtcgg
    19681 ctccacctgg gaaaacatcc tttcggctcg cttcagaaaa gccacctcgg ccctcaggcg
    19741 tttctttccc acccccctga gaggattggc ccatactctc gtgaagcaaa cccccttttg
    19801 ggacccctcc tgctgcatct tctgcggagc ctgcgaaaaa gtctgcccgg cgggcgcaat
    19861 tcgaatcaat tgcagtacgg tcctgatcga ccccgaaaaa tgtgtacgct gcctttgctg
    19921 tcacgaaatg tgtcccgtcg gggcgatcgg catccgcaga aacatccttg ctcgattcct
    19981 gctccgccag aaatgaaacg gccccccgaa agaacgccgg ggggctcatg aggtttgaag
    20041 tcggtcaaat ccgtctttct attggttttt ctccttgagc ctgtcgagaa cgatcttgta
    20101 accgtcggcc ccatactcga gaaatttctt gacccggctg atcgttgccg tgcttgcacc
    20161 tgtctgctgg gcaatctggg gatacgtgta tccttcgctg agcagcctcg ccacttcaag
    20221 ccgctgggcc aacgcacgaa tttccccaat cgtggcaata tcctcgagga agcggtaaac
    20281 ttcctcttcc gtttccagaa cgaggatagc cttgcaaagc tgatccgtca gacggtcctt
    20341 ccatttttcc gccactcggt tcacctccat tatttatacg caacagtttg cgctatttgg
    20401 actgattata acataaactt tacttcgttc aagcgctgat acaccgtgat ttcgaatcgt
    20461 tccgttcctt tccgttcagt tcagttcctg cagcaagttc atgaacatcc ccagttcctc
    20521 ctcccggccc accgaaaccc tgaaccagtc tccgggttgt tcaagagtga ggtacttgag
    20581 gcggatgcag gcttcttcca tcctttcgcg gacccgggag atcgggatcg gggatcggac
    20641 aagaacaaag ttcgattcgc tttcaaaagc tctccagcct gtgatccgat tgattccctt
    20701 cacaagctgg tcccgaaggt accgaatgct caggacgcgg ctttccatcc actccccgta
    20761 ttttaaaacg attttcgcga tttcctgcgg aagcagaccg acattgtacg ggtccctgac
    20821 actctctacc ctttccgcca cggaagggga gcaggcggca tatccgattc gcaggcccgc
    20881 catcccccag gcttttgaaa gggttttcag gatacacagc cgttcgggca gggttcgatt
    20941 ccggaaacat tcgagaaagg tttctcttgc aaattccccg tacgcttcgt cgatcacgag
    21001 gacccccttg aatcgttccg aaaattccga aaggaaatcg ttgggaatcc ggattcccgt
    21061 cgggttgttc ggcgtgtcca gaaggacaag agcgggagaa caggaggaaa ctgtttccag
    21121 aaagcgctcc tggtcgaaaa cgatccggtt ttcctcgaaa cgcagcggga cgggcaccca
    21181 ttccacctta tgccggcgac agagctcggg gtattgcgtg aaggtcgggg atagtgtcag
    21241 gaccttttgt cccttgtcgg tgaaagccgt gaaaagaaga tccaggattt catctccgcc
    21301 gttgccgaca acgatttccc gccaggaaat ccccgtcatt tccgaaagga cctgcttcag
    21361 ggctcttgcc tcaggatccg gatagcggtt caggtcgatg ccagaaacga cctgtctaat
    21421 ctgctctctt aatgaggggg gcaaccggaa aggattctcg ttcttgtcaa ggaggatggt
    21481 cgtgtcttct ttgcactcaa aacccgccag gtgcggcctt ccgtacaagt ccttcgcctc
    21541 cattgttatg ctgtctaggt aaagcggtgc ttttttcaca cggaggagag tttaccagct
    21601 tttccaatgc ctgtcaaacg gacaagtcct gcgtttcaag ttccgccgcc tgttgacgcg
    21661 gcaggaaacc ctgcatataa taggtcagcg ctggccgggc tggcggaatg ggtagacgca
    21721 cgggacttaa aatcctgtgg acgaaagtcc gtgcgggttc gattcccgcg cccggcacca
    21781 gagagcattg atcgcggggt ggagcagtca ggaagctcgt cgggctcata acccgaaggt
    21841 cgcaggttca aatcctgccc ccgcaaccaa tggcggtgta gctcagttgg ctagagcatg
    21901 cggttcatac ccgcagtgtc agtggttcga ctccactcac cgccaccaat cgaatatatt
    21961 ttcctgaaag gcgggccgaa acccgccatt tttgttccct gacttctctt tttcagaagg
    22021 gggaagcaat ggaacaccct gtcattcaaa acgcaagagc ggctgggaaa gaccagggct
    22081 ggatcgaatc gactgaacct cttgttgctg cggtttcggg cgggggggat tcagtggcgc
    22141 tttttttcgt ccttcgggaa atctaccggg ggccaattat cgtcgcccat ctcgaccatg
    22201 ggatccgccg cgtttcttcc cgcgcagacg cggaattcgt ccttcaactg tgcaaggaga
    22261 acgggatcga ttgcgtcctt cgaagcgctt gcatccccct tcagaaagag aggggcgaat
    22321 cccttgaaga ggcggcaagg cgaatccggt acgctttcct ggaagaggtc cgcagaaagg
    22381 tccgcgccgc ctggatcgct attggccaca attctgatga cacggtggaa acgatcttgc
    22441 tcaaccttct tcgaggaacc ggactggctg ggctctgcgg gcttcccgag agaaggggtt
    22501 ccattatccg gccgctgatt cattccaccc gctgcgaact ccgtcaattc ctcagggaaa
    22561 ggggcttctc ctggagagag gacgaatcga acctggattc ccagtacctg agaaaccgtc
    22621 ttcggaacga attgatcccc ctgctggagc gggattacaa cccggcggtg gccagaaggc
    22681 tcctttccat gagaaaaact ctgattccct gcagggaaac tctcgaggaa agaggaagaa
    22741 atgcggcagc cttcctgagg agggaattac cctgcgtggt ttcttcctgg gacctgtccg
    22801 ccgttcgtcg cctcgatccg tcgacttgcg cggaagtgtt ccgctccgaa gcggcgaggc
    22861 tggggctgaa aacgctggat tgcgaccgtt tctcttgcct catgggattg atccactcca
    22921 gccagggctg gcgtttccag tgggaaaaat cgcttgagat cagggcgggg agcggctatc
    22981 tggcactcct ggacaggaag atgtttgacc ggcctcccga ggaacccctt gttttggaag
    23041 aaccatccgg aacggcacga tggggtttgg gtaccttccg gtgggaaagg acagagaagg
    23101 gaaaactcgg gttttcggat ttttccgcat tccttccggc tctgccggaa aaaccggtca
    23161 tccggtcttc cattgatgca gttcgctatc ggggaatcga aaccatgccc tggttttacc
    23221 gaaagggctg gcctaccgtt acaattggcg atagaatgtc gtggacgccc ttctggagca
    23281 aagcctgcaa cttttttgaa atcgaaacga attctccccg agtccggatt cagttttatc
    23341 cggaagcggg cgcgggagag ctgggaaatg gagtatgagc taggaagaat ccttctggac
    23401 ggccgaacca tctccgccag ggttgcggag ttggcagccg aagtgtctga aacctacgcg
    23461 agcaaagaat ttgttgcggt cggcgttctc aagggagcga tctttttcct tgccgacctg
    23521 gtcaggtcct tgccgcgaaa cagccttgtc cggctcgact ttatggcagt ctcgtcttac
    23581 ggcgcctcca caaagtcctc cggaatcgtg aaaatcgtga aagacctgga taccgacatt
    23641 cgtggccgtg atgttcttct cgtcgaagat atcctggaca cgggcctgac cctttcgtat
    23701 ctcctcgatt tgctgaaggg caggtccccg cgcagcttgc ggacctgcgt gcttttggac
    23761 aagccggacc ggcgaaagat cgatgtccgg gtcgatttca ggggatttcg gatccccgac
    23821 gagttcgtgg tcggatacgg attggattac tcgggatatt ggaggaacct cccggatatc
    23881 catgtagtga acccgatcga agccccggcc gggaacatcc aacccaagga gtgaagtgca
    23941 cgtgaatcga atcgcgaaaa acctgggcct gtacctgatc ctgatcgtcc tggtggtcag
    24001 cctcgtcaac gctttcctga cccccagcca gaacagtcag gaaagcactt cgctaagcta
    24061 tagcgatttc atcaagtccg cccaatccgg tcaaatcgaa aaggtcacaa tcgaagaaaa
    24121 ccttgtcaag gcgacctcaa aggacggcaa gcatttcaag acttacgtcc tggatcccgg
    24181 ccccctcgcc caagacctcg tgaagctagg ggttgcagtc gagttcgtcc cgccgcaaaa
    24241 ggccccctgg tgggcgggca tgatgacctc gcttttcccg acgcttcttc tcatcggggc
    24301 ctggatcttt atcctgtaca acatgcaggg cgggggaagc aaggtcatga actttgccaa
    24361 aagcaaggca aagcttttct tggacaaccg cccgaaggtt accttcaagg acgtcgcggg
    24421 ttgcgatgaa gccaaggagg aactgcagga agtgatcgag taccttcgtg accccgagcg
    24481 gttcgccaga ctaggcgcca atgtcccgaa aggggtcctt ctccttgggc ctcctgggac
    24541 cgggaaaacc ctcctggcaa gagcctctgc gggcgaggcg gatgtcccct ttttcagcat
    24601 cagcggttcg gatttcgtcg agatgttcgt gggcgtgggg gccgcccgtg tacgagacct
    24661 tttcgagcag gcccgcaaaa tgcagccctg catcgtcttc atcgacgagc ttgatgccgt
    24721 aggtcgccaa cgcggcgccg gcctaggggg cgggcatgat gaaagggaac agacgctcaa
    24781 ccagatgctg gtcgaaatgg atgggttcga cgcaacaacg ggaatcatcc tgatcgccgc
    24841 gacgaaccgc ccggacattc tggatcctgc ccttctccgg cctggacggt tcgaccggca
    24901 cattgtcgtc gacaagcccg actggaaggg gcgccaggcg atcctggagg tccattcaaa
    24961 agggaaaaaa cttgaagagg acgtccgtct cgacatcatc gcgcggcgga ccactggttt
    25021 tgtcggagcc gacctggcta atctcgtgaa cgaagcggca ctccttgctg cgcggagggg
    25081 cagaaaggcg cttgccatga ccgattttga agaagccatc gacagggtta tcgcaggtcc
    25141 tgaaaggaga agccgcctga tcaacgcaaa agagaaggag atcatcgcct atcacgaatc
    25201 cgggcatgcc ctgatcgccc ggctgatccc cggttgtgat ccggtgcaca agatttcgat
    25261 cattcccagg ggacatatgg ctctcggata cacattacag ctgccagagg aagatcgctt
    25321 cctcatttcc cggaaggagt tgatcgaccg catctgcgtc cttctcggcg gacgggttgc
    25381 cgaagaaatc acccaggggg acgtgacgac cggcgcccag aatgacctcg aaagagcgac
    25441 gcagcttgct aggcagatgg tcaccgagtt cggcatgagc gagaagctcg gccccatgac
    25501 cctgggcagg aaacaacacg aggtgtttct cggaagagac atcgtggacg accgaaatta
    25561 cagcgacgag gtggctttcg ccatcgacca ggaaatgcgg agcatcatcg atggttgtca
    25621 cagccgggta aaaacactcc tctccgaaaa cctcgatctc cttcataggg tgacgcgaaa
    25681 cctcctcgat agggaaatcc ttgagggaga agagctggaa acactgatcg ccggagggga
    25741 tcttcccccg ctttcagaaa gcggcggcac ggcttcgaag cccgcaacgg gggttcgtga
    25801 ggaagcggca gaagggactg aaccgctccc gggaggacga ggcaatccgg agccggaacc
    25861 cgctgcatga catcctttgg gttgaagggc aatgaagacg cacgtgacac tcgtgcgtct
    25921 tcattttttg gagaaaccgc aggaggcaac gtttgggaat tttcaagctg aactgtccat
    25981 ggccgccggc gggtgatcag ccccaggcga tcgaagccct ctcgcagggg cttgccaaag
    26041 gcttcccgat gcagacgctg gtcggagtga ccggaagcgg gaaaacctat acggttgcaa
    26101 acgtcatcgc ccgtttcgac cgccccgttc tggtcctggc gcacaacaag acactggccg
    26161 cccagcttta taccgagttc aagacctttt tcccggagaa cgaggtccac tattttgtca
    26221 gctactacga ttactatcag ccagaagcct acgtcccggc ccatgatctc tacattgaaa
    26281 aagacgcttc gatcaacgaa cggatcgaga gattgcgact cgccacaacc aaggccctta
    26341 ttgaaaggcg ggacgtcatc gttgtcgcga gcgtgtcatg catctacgga cttgggaaga
    26401 agaaggccta cgaagaagcg gtcttcggct tcaaggtggg agaacaatgg acgcggagag
    26461 attttttcca atccctcctg ggaaactatt accagcggaa cgattccgtt ctggaacctg
    26521 caaaattcag ggtaaggggt gacgtggtcg aagtctttcc agcctacagc gataccgccc
    26581 tacgcatttt ttttgatgac aggagcatcg acaggatcga cgaaatcgag ccggtttcgg
    26641 gcaaggtcct ccgcaggaag gaacaggccc tcatctttcc agcccagcat tatgtgacaa
    26701 caaaggagaa cattgcccgc gccctgcagg ccatcgaatc ggaattggag gaacagctcg
    26761 cccggatgac ggcagagggc cggttcctgg aagctcagag gttacgggag agaacccgct
    26821 acgacatgga aatgctggcc caagtgggct attgctcggg aattgagaac tattcccgac
    26881 acctggaggg gagagaaccc ggcgaacccc cgggaacact cctcgatttt ttccccccgg
    26941 actttatcat ggtcattgat gaatcccaca tcacgatccc ccagatccgg ggaatgtaca
    27001 acggagaccg ctcccgcaag gaaaccctcg tccgctacgg attcaggctg ccctcaagcc
    27061 tggacaaccg gcccttgaaa tgggaagagt tccgggggta catgaaacaa gtgatctttg
    27121 tttccgcgac tccaggagat tgggaaacga accattcatc gcaggtggtc gaacagctga
    27181 tccgtccgac cggggtcgtg gatcccgaag tcgttgtggt ggaagcgaaa aaccaggttg
    27241 acgatctgat tggaaggctt tcagcactag accgcaaccg cgagagggcc ctcgtcacaa
    27301 cccttacgaa gcgctcggcc gaggatctgg cagaatacct ttcggaagcc gggttgaaag
    27361 ttcgatatat ccattccgag ctggacacgt tcgagagggc cgaccttttg aagcacctcc
    27421 ggttgggaga gattcaagtc ctggtgggag tgaacctcct ccgcgaaggg ctggatctcc
    27481 ccgaagtttc tctcgtggcc atcctcgatg ccgacaggga aggcttcctt cgctcccacc
    27541 gctccctcat ccagatgatc gggcgggctg cccgcaacat cgccgggacg gtggtcctct
    27601 acgcggatac aatgaacgac agcatccgca aggccgtgga agaaacgcaa cgacgccgtg
    27661 aaatccagcg gacctacaac gagtccaggg gaatcactcc gcgaacgatc ctgaaagagg
    27721 tggtcgggat tcttcctggc ggggtggaag aaaaggaagg cgaaacggct ggtttttcgg
    27781 agaccgagtc tttttgttcg gaggatcgct cgattctcga agagaaaatg tggaaagcgg
    27841 tcgaaaggct cgattttgag gaagcggcta gaatcagaga tttgctggct tcaggcggga
    27901 aaggagatct gaagggtgga ccatcgcatc gtcgttcggg gagcaaggga acacaatctg
    27961 aaaaacattt ccgtcgaact tccaaaagat aaactggtcg tattcacggg gccgtcaggt
    28021 tcagggaaat cctcgttagc tttcgacacg attttcgccg aggggcaaag acgctatgtc
    28081 cagtcccttt cagcctatgc tcgccagttc ctcggagtac taaacaaacc cgacgtcgac
    28141 gacatctccg gcctgtcccc gtccgtttcg atcgagcaga aaggaatttc tcacaaccct
    28201 cggtcgaccg tcggcaccgt caccgaaatc tatgattacc tgcgactgct tttcagccgg
    28261 atcgggactc ctcactgccc gctttgcggg gaacgggtcc accgctactc gatcgatgaa
    28321 atcctctccg tgattcttcg cgatttttcc gggaaacgcg ttgaaatcct tgcaccgctt
    28381 gtcaggggaa agaaaggtga acaccggaac gtcttcgagg aagcaagaag ggaggggttc
    28441 cttcgcgttc gggtggacgg aagagtcctg tggctggagg aagaaatcgc cttggaccgg
    28501 aaccggaaac attccatcga ggtcgtcgtg gaccgcctga cggtatcgtc agaaaaccga
    28561 gaccgcttga gcgaatcgac agaaaccgcg ctccggatcg gaaccggttc cgtccttttc
    28621 gcaacgccag agggagagga aaagctgctt tcggaaaatt tcgcctgccc gaaatgcgaa
    28681 atttcccttc cagaaatcga accccggctt ttttccttta acagcccgtt cggagcctgc
    28741 caggaatgct cggggctcgg cagtcacgaa catttttccg tggaacttgc cgtcgacccg
    28801 gaacgatcgg tctgggatgg agcgctgctc ccgtggaaaa caactcatta catgctaaga
    28861 aagctgaaag ccgtttgcga agcgaaaggc tgggatcttt ccaagccatt cagggggctc
    28921 ccgccggaaa tcagggactc gatcctgaac ggagtttcgg atcgcctccc gatgacctac
    28981 cgggaaaacg gacaggtaca gacgtacctg gggcgatacg aaggcctcct tccctggctg
    29041 gaaacccggt gggccgaaac ggaatcggac gccgtaacgg aagagctgat ctcctttcgg
    29101 accgaggacc gatgccaggc ctgccagggc cgtcgccttc gccccgaagc gctggccgtt
    29161 gaaatcgacg gtttgagcat cgcagacgtg accgaaaccc cggtttcttc cctgctccaa
    29221 cggctggacc agctcaacct gtccccccag gcgcttgaaa tttccggtca gatcctcgat
    29281 gaactgaaaa agcggctctc cttcatggac gatgtcggtg tcggttactt gacgctgtcc
    29341 cgccgtgcgg attcccttag cggtggcgaa agccagagga tccgcctagc cactcagatt
    29401 ggctccaagc tttgcggagt gctgtacgtt ctggatgaac ccacgatcgg ccttcatccc
    29461 cgggatacgg aaaggcttct ccagacgctt cgggcggttc gcgatctcgg caacaccgtc
    29521 ctggtggtgg aacacgaccg ggacacgatg ctcgcagcgg actacattct cgagctcggc
    29581 cctcgagcgg gggaacacgg aggtcaggtc gtcgctgccg gtacccggga agagatcgct
    29641 tctttagatt gccttacggg gccctacctg gaaggaaggg tctccggggc aattatccgc
    29701 cccactccaa gggaaccggg cgggtggctc accgtgctcg gggcaagaga acacaacttg
    29761 aaggatttgg acgtagccct gcctctcggc gtcatgacct gtatttcagg ggtttcaggt
    29821 tcagggaaga gcacgttcat gcacgaggtc ctgtacaagg gcctaaagcg tatactggac
    29881 agggacttcc gaggacggcc tggggctcat cgggaaatcc tgggctggga agccctcagg
    29941 aacgttgtcc tggtcaccca ggatcccatc ggacgcaccc cccggtccaa ccccgccacc
    30001 tataccgggc tttttaccct gatccgagag cttttttcgc agcttcccga agccagaatc
    30061 cgtggcttcc agcccggccg gttcagcttc aatgtccgag ggggccgctg cgaagcctgc
    30121 aagggcgagg gcgaaagccg gatctccatg cttttcatgc cggatgtatt tgtcccttgc
    30181 gaaatctgca aagggaagcg ctacaatcat gaaactctcg aggtccgata cagggggaaa
    30241 aatatcgcag atgtcctgga tctgcccgtg gatgaagcct tgcaattttt cgccgacatt
    30301 cccaggatcg cctcgaaact gaaggtgatc gaggaggccg gtctcgggta catccgcctc
    30361 gggcagcccg cacccaccct gagcggcgga gaggcccaga gggtcaagct agctaacgag
    30421 ctttccaagc ggttcaccgg ctcgaccctt tacctcctgg acgaaccaac caccggcctg
    30481 cattacacgg atgtaaggaa gcttcttctt cttctccaca agctggtgga ccagggaaac
    30541 acggtggttt tgatcgaaca caacctggac gtgctggctt cttgcgatta cctggtcgat
    30601 ctggggcccg aaggtggaga agagggcgga agactcgtca cgacgggaac cccgcaggag
    30661 gtagcgcgat ccgggatcgg ccacacctcc tcctgcctga gagattttct gggccagttg
    30721 agaatcaagg acaaggagga aggaccatgc ctgaagaaag aagccaaaac ccgcaggaaa
    30781 ttgtctgggg gcgcaactcc gtcctcgccc tgatcgaggg ggctccagcc cggatcaaga
    30841 aagtcctcgt tgcccgggga actcaccatc agtccatccg gaagatcgag gattcctgcc
    30901 acgatcggag aattcccctt cagcgggttg accgcatcac gatcgagcgg gcctgccccg
    30961 gaaacgccca tcagggcgtg gtggccctga tttccccggc agaccttcac gatgtttctg
    31021 aactggttca tttcgtgccc gaaaacccgg aaccggcgtt ggtccttttg ctggatcacc
    31081 tgcaggatcc cggtaatctc gggagtatct tgcgctccgc agaagctttt ggtgcatcgg
    31141 cggtcgtgat cccgaaaagg cgtgcggccc ttccaaccgg aacggtcctg aaagccagtg
    31201 caggtgcggc ggaaaggatc cccatcgtat caaccccgaa tcttctttgc gctctggata
    31261 gcctcgaacc cgaaggattc tggtcggtcg caatggaccc tggtgcaggt gaacccctgg
    31321 acggcaagcc tcttcccgcg agaatggcgc tcgttgtcgg aagcgaaggg cagggaatca
    31381 gccggccggt ggaaaaccac tgcgatgaac ggcgcagcat tcccatgggc ggggcctcgg
    31441 gctcgctcaa cgcttcggtt gcagcggcga ttgccatgta tgtctggaaa agaatgattg
    31501 acaaggcggg aggcgcccct taaaatgcat gatgctccgg gtggttagtt cagaggaaga
    31561 acgcttcctt gacgcggaag aggtcgcagg ttcgagtcct gcaccaccca ccaaaaatga
    31621 aggcatccct ttcgggatgc ctttttcttt ccctccagct ccaattcctt tataatcgcc
    31681 acgaagccgg tacttctagg aggccctcgt gattatcgga gtcggcgttg acctatgcag
    31741 tgtttccagg attgcagccg caatcgcaaa ccgcaggttc gtcgagaaag tcttttcccc
    31801 ggaggaacaa ctctacgcag agtccggagg aaaccccgcg cttcattatg cttcgtcctt
    31861 tgccgcaaag gaggctttcg cgaaggccgg cgggtgggga ctcgccagaa ccggcctggc
    31921 ttccgtgtgg gtctctagga aaagcgggcg accggaaata cgcctgtctg accgggcaaa
    31981 aaggctgttt cccgatcgga cgggccgaat ccatctcagc ctttctcacg aaggcgatct
    32041 tgtagtcgcc gtagtgattt tggagggtat caatgatccc cttctttgat gcgaacacga
    32101 ttcgcgaagc agaccgcctg gctgaagaac agttcaattt ccctccaccg ctgctcatgg
    32161 aaaacgcggg aaagggagct gccgaggcta tcctcgaact ttacggcaaa cgagactgga
    32221 caatcctttg tggacccggg aacaacggtg gggacgggtt tgtggcagcc cgccacctgg
    32281 cgcttgccgg ctgcgaggtt accgtccttc tcacggaaga tcgagccagg atcaaaagtc
    32341 ttcccgcagc caatctcaaa cttctggatc agatgggttg tcccatgctc caggttgcag
    32401 aacacagcga tgagttccta gcggaacggt tgcgaaaatc cgccggcatc atcgatgctc
    32461 ttttgggcac tggctcatcc ggcactctgc gcggggaacc gctcaggctc cttcggctag
    32521 tgccggaaaa cggccccgtg aaagtcgctc tcgatgtccc cacaggggtg gacgcttcaa
    32581 cgggcatagc ggatcccgaa gccttccggg cggacctgac gctcaccttc ctcgccccga
    32641 aaatcggcct gagggtcatg cccggagccc tctgcgccgg aaagatcagg gtcatcgatc
    32701 tcgggatcga aaagaaactc cttctcccgc ccccttcggt cgaaggctac ggcattgagg
    32761 acgcggctcg ggactggccc tccgtatcct tcagtgatca caaggggaag aagggaaccg
    32821 tcctgatcct cggaggatcc tcgctctacc gcggggcacc aatccttgcc gctcgcgcag
    32881 ccttgcgagc cggagccggc ctggtcgtcc tggtcgtccc ggaatgcgtt gcgagatcgg
    32941 cttcctctgc gctaccggaa gccctcgtgt tgtccgcctc aaaagaagat ggggggcatg
    33001 tcgattttga ttcggcggtc caaatccttt ctaattggtc tgaaaaagcc ggttgcctga
    33061 tcttcgggcc cggctcaggg cgttccacca caaccgggtt gcttctggaa tggatttccg
    33121 cctcatggca gaagcccctt ctgctagacg ccgactccct tttctttctc caggggagag
    33181 tctccgcttc ctcttgcctc attacacccc acgagggaga agcgggccga cttctccgga
    33241 aaccgacggc tgaagtggcc aaccggcgcc ttgaaagcgc atccatcttg gccgcccgtt
    33301 tcgggaccgt ccttctgaaa ggcccctact cgatctgttg cgaccagaaa agaatcggaa
    33361 tcgtcctcga atcgacacct tccttggccg ttcccggatc gggggatgtc ctgtccggaa
    33421 tcgcgggaac ccttctggcg aaagggcttt ctccgtggag agcgggcctc gcggcctcct
    33481 gggctcacgc cagggcaggg aaactcctga gtgattcccg cgggaccgaa accgggttgc
    33541 tcgcaggcga aatcgcggat tcactccacc tggtttttcg cgaactcgct gtcttctcca
    33601 aaaacgggag atcctcggtc aatgccgaac tctgccagcc cggaatttga gcttgttttt
    33661 ccttccagtg gagagagaga aacctccagc ttcgggaacc tgctttcccg gtttgtcttc
    33721 ccaggccttc tcatccttct tcacggagac ctcggggcgg gaaaaaccgt cctcgtcaga
    33781 ggcataaccg agggtctgtc ggccggaaac gtcaggagcc cttctttcac tctcgtcaac
    33841 gagtatcccg cggccatccc tgtgacccac gcggaccttt accgtaggcc accgggcggt
    33901 caggaggatc tcgctctgga agaagcgttg gaagaggggc gcctcgtcct ggtggaatgg
    33961 gcggaaaact ggaaacaccc cccaattgaa gaagcctgga tctgcaggat cgaacttccc
    34021 gagcctcccg actctacgga ccgccgcaca atcaggatct tctccttcgg tgagaaggct
    34081 actttttccc tccgggaggc ggctacttcg attctgaaaa aaggagaaga attggattga
    34141 cgcttaatct tgcaatcaac tgctccacca cctggacatc gatcggcctt tcagaaaacg
    34201 gaacggttct tgcggaaatg aacctgaata tcggcagggg acaatcctcc gaacttccga
    34261 aggcggtcga ggaaattctc cggcattccg gccgatccct tgcccaggtt gacttgttcg
    34321 tcctgacgat cggccctggc tcctttaccg gggtacgcgt cggtttgagc tacggactcg
    34381 ccctggcagc cgcaaggcga tgccaggtgg ttcccgtgaa taccctcgag ctgatggctt
    34441 accaggcagc cgccgttttc cgcggaaacg tcctgatccc aatcctgaat gcaaagtgcg
    34501 ggttttttta cgcagcggcc tacggcttta atcaagaaac gaagcgcctt tctggattct
    34561 gggaacctgg attctattcg ttttcgcaat tggacgaacg gctttcttcc tgggaaggca
    34621 aggctctttt aatacttgaa aagaccaagt cctttccgct tttttcaaac cccggtttcg
    34681 tttatgaaga ctacatccct tccggaggaa ggctcgctct ccttggcgac ctttttttcg
    34741 aaagagccgt tgctcccgaa tcagtcaagg gagtctatct ccgccctcca gatttgggat
    34801 aaatcatccc ttgcgattcg ctcctgaaaa tgctatatat gcttgaggcg tattctgtac
    34861 acacgtttaa atttttggtt aacagtaaac taggctgaaa ggagggttta ttcaaaaggt
    34921 tttacggctt ctttttccag acctgctggg gaggtgtggt aaggaaatgg acctgatcaa
    34981 ggagcatccg atcctggaat ttgagcacgg gaaga
//
LOCUS       DAOOVN010000009        32487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_913_length_32487_cov_14.773865, whole genome shotgun sequence.
ACCESSION   DAOOVN010000009 DAOOVN010000000
VERSION     DAOOVN010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 32487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32487
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_913_length_32487_cov_14.773865"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(226..2067)
                     /locus_tag="PLY83_02070"
     CDS             complement(226..2067)
                     /locus_tag="PLY83_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HOV28852.1"
                     /translation="MRQAFRTWVDLSSRSAKGETLSEKETRTWGGERGLALPVLEKRL
                     TRETDALSPENPLVIAAGLLNGLAFSGTCRYGVYAKSPLTGALGESEAGGSFGPAMRA
                     QGVDAIVLESASENPVYLWVENGTVSVLDASDLWGKTNRETYEALTARHGRISALLTG
                     PAGERRVRYACIINDLRHANGRTGLGAVLGSKRVKAVAAPAPKAIPPVVPERLKEAQA
                     LFSSWKENPLAWTLRETGTPAALGGLNALGILPTRNFREGSFEGADLMNGAFYNRELL
                     AQTHGCFACAIRCKRVPRPGARNLDPAYGGPEYETVAAFGPACGCSDLSAAAKAHERC
                     NALGLDTISTGMTIAWLMEAVERGLISPEKAGVSGFGDTEGMLSLIEAIAFRQGIGDI
                     LAEGSKRASSILGFGADFLMEVKGQELPMHDPRGKVGVALGYALAPGGADHMQMAHDT
                     LMTDPEGFGTKSVAPLGILGPMDPLSLGREKISALMTLWTFWTFQNHLGGCHFVFAPR
                     SAFPVEGIPLLVEAATGWKTSLWELMRMGQRSLAAARILNRNLGFGPADDVLPKRLFE
                     PISSGPYEGRRTLDPETFENALKMAWAILGWDEEGNPRPGTKAALGL"
     gene            complement(2075..3214)
                     /locus_tag="PLY83_02075"
     CDS             complement(2075..3214)
                     /locus_tag="PLY83_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HOV28853.1"
                     /translation="MREFRIAVLYASVGTGHKTAALALTKWFQAEFPGVKTVCLDTLA
                     YSSPLVRGIYTRSYLEMVRRAPQLWGYFYESMEHPESQDGLLSTLNELAEKLNLTKLR
                     LSLESFAPNVILFTHFFGTSAVMSAFPGVPVYYVNTDFLSHIFHRNRAFSGWFVASDE
                     AVRQYAADGLDLGRVVLSGIPIDPLYCTPPSREEARKRLGLERDAPAVLVVSGGIGVG
                     PIEGVVESLVRQDGMDVFAVCGRNEELAEALRTRFEDEPRVRVEGFVNGLIDWYCASD
                     LVIMKPGGLCSSEALALGKPILIMDPIPGQEQRNSDILLDSGAARTLLEYRAAGEKAR
                     QILENPAELARLREGARRLAKPFAGRTVARTLAEWHGIEPNGQKE"
     gene            complement(3219..3413)
                     /locus_tag="PLY83_02080"
     CDS             complement(3219..3413)
                     /locus_tag="PLY83_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV28854.1"
                     /translation="MSEEKPFCKRITPGFLKKNLEEYKEWVREPLYVCRKCGRVARRS
                     RDLCKPVPLQGAGEPSASDE"
     gene            3648..4811
                     /locus_tag="PLY83_02085"
     CDS             3648..4811
                     /locus_tag="PLY83_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOV28855.1"
                     /translation="MKKKVTVLGCGLVGSVMALDLAADEAYEVTVCDASLENLSRTKA
                     RAKGPLAIRTDVDFASPASITEAVRGQDLVIGAVPGFLGYAMLGAVIRAGVNMSDISF
                     MGEDYREWDAEAKKAGVTTFEDVGVAPGASSILIGHACEQLDRVDDVTYSVTGLPTRP
                     EPPFNYKLVFSPDDLIEEYVRPARMKRDGKIVTVPALSGCRIMKFDIPGIPLPEMEGF
                     FTDGCRTLLDTIPSPNVTEYTLRYPGTARDMTLLREIGLFGTEPVDVKGTKVAPRDLF
                     GALAYPRMKLGEEENEFTFFHVEVTGEKDGKRLQYTYTLYDEKDMATGFPSMSRTTGF
                     PCVIVGRLLAEGVLNMPGVNPPEAVGKNPEAVRRLLEELQKRGVKIASSVKEL"
     gene            complement(4858..5958)
                     /locus_tag="PLY83_02090"
     CDS             complement(4858..5958)
                     /locus_tag="PLY83_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HOV28856.1"
                     /translation="MPRIRPEAEGFSGSVLGYNLVEKFPGGESRVEKTVTHIISHTDL
                     DGVTAAAVAWAAAWPESAPVKITLTGYGEVDNLLLESLQAGQKPLVLDLFCQRQQTVD
                     ELDTRYVPGDLPFLFDHHETTFDRYGNRPWAVVDISRCAAAVYHAWLLEQPLSASVRQ
                     RVERMGAIVNMANDRDLWLGQEPDSRLWQAMVTLCGPWGVLMRLAADPDAALTESERE
                     VAERFVRAQEERFARALERVLRVRDELVYVPPDLLEFGDVSDFCGLVLDRMENPPALV
                     AVPARRPAGDWAVSMRSRDGLAGRITGLLKDGRKVRGGGHGDAAALYFPPHYTADQIR
                     ESLLSALRTAREADRPVGLSLGDLLKEKLNGK"
     gene            6230..>6888
                     /locus_tag="PLY83_02095"
     CDS             6230..>6888
                     /locus_tag="PLY83_02095"
                     /inference="COORDINATES: protein motif:HMM:NF012529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HOV28857.1"
                     /translation="MSKEAQVLWQDIIRATREESPGGAWEIWMRTCEPLEVVDGTLVL
                     DVPNVFVKEQIQSRFLEALNDSARKRGDLRGVELRVSSEVKPAEQQRAEKAASPPPVS
                     RHGLNPNYVFDTFVIGKSNRLAHAASLAVAESPGVAYNPLFVWGGVGLGKTHLMHAIG
                     HYLLNKDSGAKVCYVSSEKFTNELITAIKDNRTHEFRNRYRSVDVLLIDDIQFLANKE
                     ST"
     assembly_gap    6889..6988
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6989..7411
                     /locus_tag="PLY83_02100"
     CDS             <6989..7411
                     /locus_tag="PLY83_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOV28858.1"
                     /translation="TNIPRNIRELEGSLNRIVACAELNNEPMTVENAASWLKDIIRHV
                     AKGPVTIDLIQQYVAEGFGLSVEDLTSSKRTAEVSLARQVAMFLSRTHTEASLQQIGY
                     AFKKKDHTTVIHACKKIGEILKSDPKIKNFVDNITEKL"
     gene            7650..8804
                     /gene="dnaN"
                     /locus_tag="PLY83_02105"
     CDS             7650..8804
                     /gene="dnaN"
                     /locus_tag="PLY83_02105"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOV28859.1"
                     /translation="MKLRIEKNAFVKSWQMAERNTSTTSTLSVLSGIRCLASGSSTTL
                     QATDLKTSVTCRAAGVSVEEPGEVVFPIKVVGELFKKVPADSFIVEVREGKATLLAGK
                     SRYRFTTYPADEFPKLPVSTAARLLFDTTVNDLERLLDEGTFSGSPNEEFPQYLGTAL
                     FQVGGNRVRVVSTDGRRLSLSMAEIPGKEGGEETRVLLPLKGLNELKRVLGTMDDQAP
                     LRVLVDDAQAYFQAEGVEFSVRRVDSSFPPYEKILTKDRTTWMTIDRMSFVSALERAE
                     LVVRDFSRMIVMNLAPGGSLKITARAPEVGTASEEVDADIDGEPLRIAFNARYLMEGL
                     KALHGSLAHLCFNGPNGQMSLSRPGEENFLYVLMPITLPEEEPVPGEDAL"
     gene            8794..9855
                     /gene="recF"
                     /locus_tag="PLY83_02110"
     CDS             8794..9855
                     /gene="recF"
                     /locus_tag="PLY83_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660078.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HOV28860.1"
                     /translation="MRCSWFQTFEFRNLKPQRLEWALGLNLLRGENGAGKTNLLEVLR
                     LLSGWGPFRGRSASEAPRWEGTAGRAALKGGFSGEEDLEIAAFVGRRVDLRRDGKPVG
                     AATVRNRVPALSFTPEDLALVEGAPAIRRRFLDGLCALLLPLYAFRLLEYRKALRQRA
                     AALRQNRGVGATAKVLAPLASWIWASRAAAVDLLRMGLSEVSELLPGPVEIAFSRGGS
                     VGLEDGLEDYWTSLARHAHLEGRIGLPVVGPHRDDLLVASGGKSALQVFSRGHRRRVA
                     VALMMAAGWAVERKLRRAPLLLLDEVAAELDDTGRSVLVEALKARGWQVFAATAESAF
                     DGWPGAVWVVSEGSVQRNA"
     gene            9871..11745
                     /gene="mnmG"
                     /locus_tag="PLY83_02115"
     CDS             9871..11745
                     /gene="mnmG"
                     /locus_tag="PLY83_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOV28861.1"
                     /translation="MMERYEVIVVGAGHAGCEAALAAARMGCSTLLLNLYIDNMALMA
                     CNPSIGGPAKGHLVREIDALGGEQARATDASTLHIRWLNTSKGPAVQTLRAQCDSRAY
                     HSAYLNAVERTPNLDLFQDIVTEIVVSKGRVRGVKTRLGLTFPGEIVILATGTYLGGR
                     VHVGLVNFPSGPLGQMPATELTKSLVKAGFEVGRLKTGTTPRIHAASVDWASLEPQES
                     ASEPLAFSHWSQPRIHVGHACYQTRTTPRTHEIIRGALDRSPLFTGAIEGVGPRYCPS
                     IEDRVVRFPDRDSHIVFLEPVGGQTAEIYMQNFSTSLPFDVQVEMVRSLPGCENARFL
                     RPGYAIEYDFLPPTQLFPWLETRIVRGLFCAGQINGTSGYEEAAAQGLLAGINAARTL
                     RGEEPVVLGRGEAYIGVLVDDLVTKGTQEPYRMFTSRCEHRLILRHDNADRRLAPIGR
                     KLGLIDDDRWRVLEARWEALEAEKARLERTRILPTEENNALLRQAGTVPIEMPVPALE
                     LLRRPEVTWDLLQAMAETPSSLSADEAATLQTEIKYAGYIEREKRAVERLERIERVAI
                     PEGFDYDSVTGLSSESRGKLSRFRPLTLGQAGRISGVTPADVQILWVAVKAYHERESS
                     "
     gene            11726..12226
                     /locus_tag="PLY83_02120"
     CDS             11726..12226
                     /locus_tag="PLY83_02120"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOV28862.1"
                     /translation="MSANPARGKRQAKDLSPIGAFLGRGLSPQARMRLNLADLSKNWE
                     KVVGEVLARKSRPVALEDGILVVACETSSVAQEILVRRDLIADRARKEWGLALKGVRP
                     SVRRIPLPRSAAVKKESPPPFEPPRHEVEKALARIGGRIGNPEIETALARLMVTFRRR
                     FSRGKD"
     gene            12291..13358
                     /locus_tag="PLY83_02125"
     CDS             12291..13358
                     /locus_tag="PLY83_02125"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HOV28863.1"
                     /translation="MGYPTLKIGKHQPRFPLIQGGMGVKISGPRLAGAVAKAGGVGTI
                     ATVGLAVDSPHYNGRNYFDANELAVKEALAEARAEAPEGVLAVNAMVALTDYERHVRA
                     ACEGGTDIVISGASLPLKLPEYVKEFPDVALVPIVSSTKAANLILRRWEKQYGRIPDG
                     FVVETPLHAGGHLGATKLEQVTDPALALEVVVPELVAFLRDEVKVDIPVIAAGGIWDR
                     SDMERAFACGAKGVQMGTRFACTVEGDASERFKQAYIDAREEDVVIIKSPVGIPGRAL
                     KTPFVAKYLEGDVTSKPCIANCLSHCSYRNDRSAFCIAQALVDAYRGDYENGLFFCGD
                     NVVKCTRIETVPEIMKELFGD"
     gene            13493..14902
                     /locus_tag="PLY83_02130"
     CDS             13493..14902
                     /locus_tag="PLY83_02130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOV28864.1"
                     /translation="MLEIPTPDTLLIQDPKHVQAFLLEVQEIARMGSWIWEPGTDCLV
                     FSSPLASILGLPRSGRTTMEEFMARIHPEDRSRVARVRSEALSSGADGYSAEYRVLGK
                     DGIVHVVARGRVERNERGTPCRVYGSIQDITERKRMEEALEEERSYLAGLFEHSPDAV
                     VVTDDEGRIQKTNRAFSRLFGYTEEEAAGRLIDDLVVPERLFEEGRGYTADLSRGARL
                     DFESVRRRKDGSEFPCRGVGVPVRLSGGQLGVYCIYRDLTAERAAREDLKRALETMKK
                     AWEQTIEALASASEVKDPYTAGHQRRVAVLASAIAREMGHADTFVTGVEKAALVHDLG
                     KIEIPAELLSRPGRLSPYEFRLVQVHAEAGFRILSKIHLPWPLAEIVYQHHERLDGSG
                     YPRGLKGAEILPEARILAVADIVEAMGSHRPYRPARPLAEALETIQEGSGKTLDPEAV
                     AACLRLFLEKGFSFPEPAA"
     gene            15212..15781
                     /locus_tag="PLY83_02135"
     CDS             15212..15781
                     /locus_tag="PLY83_02135"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HOV28865.1"
                     /translation="MKRAFLFFWILLGKAQRGIMIFTSLVVVTLILIQVALRYFFKLP
                     LMGVEELATMVGFWMYFIGAANGSRERSHIKADILNAMIKNEKALFFSKAAGNLVVTV
                     LACIMVQWAIRYFQWSLRSWERSPALMIPMVYAQFSLVVSSFLMAFYFLVEFLDNARQ
                     FFGKPPFALPDDDTGFACLAAPGEQKEGR"
     gene            15782..17083
                     /locus_tag="PLY83_02140"
     CDS             15782..17083
                     /locus_tag="PLY83_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOV28866.1"
                     /translation="MHIVIDLVILISTILLGIPVPFCFMAAALYMGVIFFPDFSFLTT
                     VGFRGLNSLTLLAIPFFIMAGSLMGSAGIAERLVGVANSMLGRIRGGMGAATIVACAI
                     FGAISGTCSSAVACIGSIMIPRLETLGYPRSYSTAMVSCAAVLGQLIPPSVPMILYAW
                     VTQQSVAACFLSTVIPGLLTMTAFCIINYFMCKGMPTVRVDPPLPSRERNALVAKAFK
                     RGVWSLLMPVLILGGIYGGVTTPTESAAVAVAYSIFVGFLIHRELNARILGKSLYTAA
                     TTSGVIVLMLFFVTILGRLYTMQQVPMKIAAFLLSFSNNKIMILLMVNVFLIIIGMLM
                     DDLSGTMLSAPLLMPLMLQIGVHPIHFAAILGTNLGLGNVTPPTAPILYLGGRIGNVP
                     IDQYIKPALIFMIFGNIPIIILTTYFPALSMWLPQLLMGVK"
     gene            17227..18255
                     /gene="dctP"
                     /locus_tag="PLY83_02145"
     CDS             17227..18255
                     /gene="dctP"
                     /locus_tag="PLY83_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOV28867.1"
                     /translation="MKKWVFLSLALVLALGIVSFAAHTPAQAAPQYHWRLAQVHPVDS
                     DFDIRAKAFAKKVFDRTNGRIQIDVYSGGVLGDWTETFEMLMRGSIEMTMAQANANFD
                     PKLNLAYYFPYLVTNMKEAKKVYGQGGWAYKIIQDLWAAHNIKALAVIPVGMSGVSLK
                     SLPKDYANPSVPKGMKVRVMPIKPCELTYEALGYIATPIPYAEAYSAIQTGIADGQMG
                     GPPYQAWQFKDVNKVWIQYNDYFESWWFAINMDVWKKLSKADQDIIQKAANEESLIRW
                     ENVAKEDEEYRQKLKKEYGWQIVMLTDAQLKEVAKQVRKKVWPEMEKLVGKELMDRVY
                     KESGIPRQ"
     gene            18317..19681
                     /locus_tag="PLY83_02150"
     CDS             18317..19681
                     /locus_tag="PLY83_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOV28868.1"
                     /translation="MKNLTRNLEKCLKLVEEDRAITSPAAKVPYYPLVVKKGRGAVIE
                     DVDGNCYIDLLSSAGAINTGHCHPRVVEAIKRQTEDLILYTHVYMYHEPIIELTKELI
                     AISPGDFRKRVYYGLCGSDANDGAIKMARTYKKRPKILSFIRAYHGSTYGALSLSGVS
                     LPMHRGLGPLLPEIYHVPYPDPYRPPVPGMTPEEVSDYCIDYIRTAFSNHIPADEVAA
                     VFIEPIQGDAGLVVPPAKFLKDLRKLCDENGILLFSEEVQQGFGRTGKWFGIENFDVV
                     PDAIILGKAIASGLPLGGCVARAELMDDWEAPSHLFTAAGNPVCCAAAVATINVIRDE
                     NLLQHTAELGAHVKARFEEMQKKYELIGDVRGIGLSVGVDLVKDRETKERHREAAAKI
                     CYRAWEKGLLLSFFSGSVLRIQPPLVISKEQMDCAMDRIEEAIEDFEKGRIPDSVLET
                     VKGW"
     gene            19704..20009
                     /gene="ortA"
                     /locus_tag="PLY83_02155"
     CDS             19704..20009
                     /gene="ortA"
                     /locus_tag="PLY83_02155"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtA"
                     /protein_id="HOV28869.1"
                     /translation="MKVPKGSWVQIEQVVLKPEERAPQVPEDTKAVPLMLWVKGTLLE
                     DASLGETVEIETAIGRRLTGKLVAENPPYTHGFGRPVPELLSVGKEFRELIERSGRR"
     gene            20006..21427
                     /gene="ortB"
                     /locus_tag="PLY83_02160"
     CDS             20006..21427
                     /gene="ortB"
                     /locus_tag="PLY83_02160"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtB"
                     /protein_id="HOV28870.1"
                     /translation="MRDNSYAAVMARRNEIMKRALGLDYGEFELGGLAFDYERMMEKA
                     GYSLEEVRAIQKETNVGDTPLIELKNLTAAARALSEPGKGARIFIKDEAANPSGSFKD
                     RRAAVSCYFAQHHGYKGVVAATSGNYGAAVASQANMRNLKSIIVQELFDSRGIGQPEI
                     LEKGRKCEAYGAEVLQLTVGPELFYTFLRVLEDTGFFNASLYTPFGIAGIETLGAEIV
                     AQVQARFGRNPDAVVITNAGGGNLTGTARGLIKAGAAETKVIGASVDLSGLHMASDRD
                     FNRKSFTTGHTGFGVPFTTWPDRADVPRNAARPLRYMDRYVTITQGEVFAITEALAAL
                     EGLERGPAGNTSLTAAFAIAREMPEDAIIVVQETEYTGAGKHVTAQLTFARENGIEVR
                     RGDPADQVPGKNIIIPSDPSMIRPRDLSMRDLKASYLRNALKNRPAGSPEALTETEAA
                     FLAAEIKETPEGLAALLAQARGC"
     gene            21535..22935
                     /locus_tag="PLY83_02165"
     CDS             21535..22935
                     /locus_tag="PLY83_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOV28871.1"
                     /translation="MIEKRLLEETLALAVSYLEGLPERPVGAHDSVEALRARLKVPLP
                     ERGTDPETVVRELARDADGGLVASAGPRYFGFVTGGSLPAALAADWLASAWDQNAVLS
                     IASPVAAVVEEAVADWVLELLDLPRQSSVGFVTGCQMANFTALAAARHEVLRQAGWDV
                     EAQGLFGAPPVTVLAGEEAHATLFVAVRMLGLGADRVIRVAADGQGRMDPAALERALN
                     GVKEPLIVCLQAGNVNTGAFDPCARLIPMAREHGAWVHVDGAFGLWGRLLPELAAETE
                     GIEAADSWATDAHKWLNVPYDSGIAIVKNEAAHRASMVLAAPYYIAAPDEIRDPSQWV
                     PESSRKARGFPLYAAIRSLGREGLAEMVHRHCRQARLMADLLREDPRIEIENEVVLNQ
                     VLVRFLPENGEGDVDAFNRKVTALVQQEGTCWAGGSTWKGKAAMRISVSGWPTTDEDI
                     RVSAAAIRKVLARTPR"
     gene            22993..23499
                     /locus_tag="PLY83_02170"
     CDS             22993..23499
                     /locus_tag="PLY83_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOV28872.1"
                     /translation="MEGGVSLDGSTTAVLYGLAALGLALSWRRDRDKTRAALAKARAS
                     LLMILSPLVTVILLVALVLTALPPSFLSRLVGAQSGFPGVLLASLAGSVTLIPGFAAF
                     PMAKMLLDNGAGIVQVGVLVSTLMMVGVVTLPMERSIFGWKATLARNALAYGHSFLVG
                     LALWMAFR"
     gene            23510..23809
                     /locus_tag="PLY83_02175"
     CDS             23510..23809
                     /locus_tag="PLY83_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV28873.1"
                     /translation="MKRFPGILAATAICGFYLAAFPAQARGFFSLAGSNFLVVLSILP
                     PVFILLGLFDAWVPRERVTALIGEKSGLVGAGLSIFLGSFAAGPCTWPFPSRKRC"
     gene            23833..24027
                     /locus_tag="PLY83_02180"
     CDS             23833..24027
                     /locus_tag="PLY83_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV28874.1"
                     /translation="MFLFVGAWSTMKLPMLMFEMQSLGLRFALVRYAMSFLGIFWIAW
                     AVERLLGESGRRELLERFRP"
     gene            24273..24626
                     /locus_tag="PLY83_02185"
     CDS             24273..24626
                     /locus_tag="PLY83_02185"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOV28875.1"
                     /translation="MADLTGDKYWDGMINSALCKLFVLSALQETPMHGYALIQKIASM
                     TDGFCVPTEGGLYPVLKEFESCGCATVSSEVVEGRTRKVYALTEKGRRSLQAGLDAWE
                     KAIPHLVRALRRSGK"
     gene            24658..25560
                     /locus_tag="PLY83_02190"
     CDS             24658..25560
                     /locus_tag="PLY83_02190"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOV28876.1"
                     /translation="MNDLATSARFFFFILAELTVLFIGIDLLVNLLREYVPDERIQKL
                     MGRKGLSGYVLGAGLGAITPFCSCSTIPVTVGLLKAKAPFGGVMSFLLASPVLNPIIL
                     GMFLFLLGWKACLAYGLAAFSAAVLAGFAFQKSGLEKDIKPLGAARSGCCCGGGFVMP
                     RLTFSQKLAWSLKGALEDFRGVLVYMVIGVAVGAAIYGTVPASFVASVAGPGNPLAIP
                     AAALVGIPLYVRAETMIPIAVALSGKGMGMGAVIALIIGGAGMSIPEMGMLAGIFRLR
                     LVAAFVAFVFLTAVGAGIVFTLIA"
     gene            25743..26105
                     /locus_tag="PLY83_02195"
     CDS             25743..26105
                     /locus_tag="PLY83_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOV28877.1"
                     /translation="MLRATKDRRAEILKALAHPVRLGLVETLSDRRLCVNDLAGLFPV
                     DRTTISKHLAVLKDAGVLEDERSGREVYYRLRLPCLQEFIACLGRMSNGGEREVSLCC
                     AGQGKKEPAALERKEQEG"
     gene            26102..26347
                     /locus_tag="PLY83_02200"
     CDS             26102..26347
                     /locus_tag="PLY83_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOV28878.1"
                     /translation="MKIQILGTGCPKCKKLAELAEESAKELGLDFEIEKVTDIMKILD
                     FGVMSTPGLAVDGKVLASGNVPSPEQVKRLLLQAAGK"
     gene            26430..27599
                     /locus_tag="PLY83_02205"
     CDS             26430..27599
                     /locus_tag="PLY83_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOV28879.1"
                     /translation="MNGEIKKFLSLAAVFLVFYYLPVDSARIQGAAIEALAMMHEYAR
                     QHVLLCLVPAFFIAGAISVFVSQQAVMRYLGASAKRWVAYTVAAVSGTILAVCSCTVL
                     PLFAGIYKRGAGLGPASAFLYSGPAINVLAIILTARVLGLELGVARAVGAILFSVVIG
                     GLMAFIFRHEEAERQQAFAELPVPEAVRPLWKTVFFMASMVVFLVFANWAAPKVPVGF
                     WAAAYRVKWIVAGLALLSTIVSARMWFDREERVEWYGSTWGYALQVLPLLFGGVLVAG
                     FLLGRPGHEALIPSAWVSALVGGNSLFANFFAAIVAAFMYFATLTEVPILQGLIGAGM
                     GKGPALALLLAGPALSLPNMLVIRSIVGTKKTVVYVGLVVILSTAAGMLFGAFFG"
     gene            27637..28506
                     /locus_tag="PLY83_02210"
     CDS             27637..28506
                     /locus_tag="PLY83_02210"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOV28880.1"
                     /translation="MMFKHILVATDLRPRSGEILCGLGAMDPGLVGKVTLLHVLDERF
                     NGRLLASEKQEQLAMQKARLEGRGFTVEAETKIGVPFDLIVHEAQVREASLVVIGRGD
                     SPRWKSRIMGETALRVLELSPCPVLVCHGEGAKNPLFGDVVLGIDFSNEAHHALQAIK
                     KLARENPGAVKKVTLLHVHEQKNIDLLLKLVNREQIEQIVALEQERLEEFALSLKEAG
                     IPDVSVRMRTGKPVDEILADIEENRPTLAVLGAQGQGRSEMYRIGTTAFRVAQMASCG
                     VLVIPLSRPALPF"
     gene            28549..29508
                     /locus_tag="PLY83_02215"
     CDS             28549..29508
                     /locus_tag="PLY83_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOV28881.1"
                     /translation="MTAEKNDTDHGHDHGHSHVATGTSEGRLALSLVLNLVITLAEVA
                     GGLFSNSLALLSDAVHNLSDASSLGVSWLARRISRMERTPSHSFGFKRAEVLASLVNT
                     VALMGIGFFLLVEAVRKFLHPEPVAGGIMLAVAVVGLLGNLFTAWLLHADSKDSLNIR
                     SAYLHIVMDALSSVGVIVAALLMMAFGWFWLDPLLTLAVSLYVLRESWPLLKTSIHIL
                     MQGTPEGIRIDSLVEEVESQPEVVDMHHVHLWTTDGTEVFLEAHVTVCEEARSRTDEL
                     LARLSKRIREEFGIGHVTLQLEFSACVGERCFGLDGKKLNAKK"
     gene            complement(29578..30786)
                     /locus_tag="PLY83_02220"
     CDS             complement(29578..30786)
                     /locus_tag="PLY83_02220"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOV28882.1"
                     /translation="MRIRELRLLFLSCLLISGLWLLLWPSGAAEATAIEHWHVPAFYS
                     TVQEAEGAIRNMQDKFVGWEGLPLQRIEIDRSGLRAFGVLDYTNTRQQWVYTGLGLTG
                     GYYQPVVEPVHEEETTVVQLDKVKTMRLMQYPDLDKEYKWGVSMLVESPGGFDIKTFR
                     TPTRAYAETLFNALLSLSVAAGAKLEMPRLGVKFETLTEKDLKTKPFKELGLKEPKGM
                     KVTWISQGSPAAAGGLQEGDVIVEANDTPVENAEQFFRDLWGKFPSYNFKVLKKDGTV
                     SRFVEPFPEEKVPRVPPTLTFASSTPAAGPATSQGPIKLGFSLRLPNNEELAAMKGKP
                     GAVISSLAPGGVAEAAKLKVGDILLECNGKPVQGPEGLAPLLVRGENLFTVLRAGAVL
                     QVKIAPEVSY"
     gene            complement(30906..31598)
                     /locus_tag="PLY83_02225"
     CDS             complement(30906..31598)
                     /locus_tag="PLY83_02225"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOV28883.1"
                     /translation="MIEGWLAALSGFIQSGSPIAPLLAFLAGVITSVTPCALTSIPLV
                     IAYVGGTRQSDPRRGFVLSLAFSVGMASTFTALGAAASLFGRLIGTSSPWWTLGLAVL
                     MLMMALQVLGLFEFVPSKNALSTNLPKGVIGAFLAGVLGGFFSSPCSTPVLVVLLALV
                     AKSGNVGRGVFLLLLYSVGHCTLVLLAGTFTSFAARLSSSERMGRFNTVLKLLMGAGL
                     LLLSFVLFYQGF"
     gene            complement(31591..32112)
                     /locus_tag="PLY83_02230"
     CDS             complement(31591..32112)
                     /locus_tag="PLY83_02230"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOV28884.1"
                     /translation="MNVKNLQKPAAVKIAALLLIALAIVGIWFAKQREKARKADFAAL
                     HVTSAEELTQARASGLPVVIDFGADSCAPCREMAPVLEELSRTLRGKAVIKFVDVWKY
                     QKLAEGYPLRVIPTQFFFDREGKPFVPSDPRFGMILYSDKETKQHTLTAHEGGMTREQ
                     LLAVLAEMGVRLD"
     gene            complement(32245..32457)
                     /locus_tag="PLY83_02235"
     CDS             complement(32245..32457)
                     /locus_tag="PLY83_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine--corrinoid methyltransferase"
                     /protein_id="HOV28885.1"
                     /translation="MAYKGPEGLWGKPAPEEIDQYVLYEVEDDPQLKCWLKNDVLEWM
                     EKTTPTPEGDYLFSVEGAGRLRFRLV"
BASE COUNT         5930 a         9975 c        10561 g         5921 t
ORIGIN      
        1 acccgggtag tccggctttc ctcaaagggg atgggcgaaa aataagagac gaagtgacgg
       61 acaaggttcc acaactgaat agcgaaagcc tgtgtggggc gaggttctcg tccccgatga
      121 aagccccgga ggggtttcac agaccgacag gccgcgaagc tgcggtaacg ggtaggtaaa
      181 agggattcga gcaaggaaac ggctcgaatc cctttttttt gacgcttaga gtcccagggc
      241 ggctttcgtt cccgggcggg gatttccttc ctcgtcccag ccgagaatcg cccaggccat
      301 cttcagggcg ttctcgaacg tttccgggtc cagggtgcgg cgtccttcgt aggggccgct
      361 ggagatcggc tcgaagagcc gcttcggcag gacatcgtcc gccgggccga atcccaggtt
      421 ccggttcaag atccgcgccg cggccaggct tctctgtccc atccgcatga gctcccacag
      481 gctcgtcttc catcccgtcg cggcttccac gagaaggggg atgccttcca ccggaaaggc
      541 gcttcggggc gcgaagacga agtggcatcc tcccaggtgg ttctgaaagg tccagaaggt
      601 ccagagggtc atcagggcgg agattttctc gcggcccagc gagaggggat ccatggggcc
      661 gaggattccc agcggggcga cggatttcgt cccgaacccc tccgggtcgg tcatgagggt
      721 gtcgtgtgcc atctgcatgt ggtcggcacc gccgggggcg agggcatagc ccagggcgac
      781 cccgaccttc ccccgcggat cgtgcatcgg aagttcctgc ccctttacct ccatcagaaa
      841 atccgctccg aatccgagga tcgaggaggc gcgcttcgat ccctccgcca ggatgtcccc
      901 gatgccctgg cggaaggcaa tggcctcgat gagggagagc atcccctccg tgtcgccgaa
      961 gccgctgacc ccggcctttt ccggtgagat gagcccccgc tccacggcct ccatcagcca
     1021 ggcgatcgtc atcccggtgg agatcgtgtc caggccaagg gcgttgcacc gctcgtgggc
     1081 cttggcagcg gcggacaggt ccgaacagcc gcaggcggga ccgaaagccg cgaccgtttc
     1141 gtattcggga ccgccgtaag ccgggtcaag gttcctcgcc cccggccggg ggaccctttt
     1201 gcagcggatg gcgcaggcga agcagccgtg ggtctgggcc agcagctcgc ggttgtagaa
     1261 ggcgccgttc atcaggtcgg ccccttcaaa cgagccctcc cggaagtttc gggtcggcag
     1321 aatccccagg gcgttcaggc cgcccagggc cgccggcgtc ccggtctccc tgagggtcca
     1381 ggccagggga ttttccttcc aggaagaaaa gagggcctgt gcctccttca ggcgctccgg
     1441 cacgacgggg ggaatcgcct tcggggcggg agcggccacc gccttgaccc gcttggagcc
     1501 gaggacggcg ccgagccccg tccgcccgtt ggcgtgccgc aggtcgttga tgatgcaggc
     1561 gtagcggacc cgccgctctc ccgccgggcc ggtcagcagc gcgctgatcc ggccgtgccg
     1621 tgcggtgagg gcctcgtagg tttcccggtt ggtcttcccc cagaggtccg aggcatcgag
     1681 caccgacacc gtgccgtttt ccacccagag gtagacgggg ttttccgagg ccgactccag
     1741 gacgatcgcg tcgaccccct gggcccgcat cgccgggccg aaggagcccc cggcctcgga
     1801 ctctcccagg gccccggtga gggggctttt cgcgtagacg ccgtagcggc aggtcccgga
     1861 gaacgccagg ccgttcaaaa ggcccgccgc gatcaccagc gggttttcgg gggaaagggc
     1921 gtccgtctcc cgggtcaggc gtttttccag gaccggcagg gcaagccccc gctcgcctcc
     1981 ccaggtgcgg gtttcctttt ccgagagggt ctcccccttc gcgcttcgag aagaaaggtc
     2041 gacccaggtt cggaaagcct gtctcatgcg cgtgtcactc cttctgtccg ttcggctcga
     2101 tcccgtgcca ttctgccagg gtcctggcca cggttctccc ggcaaagggc ttggccagcc
     2161 gcctggcccc ttcccgcagg cgcgccagct cggcagggtt ttccaggatc tgccgggcct
     2221 tttcccccgc cgcccggtat tccagcaggg tcctggccgc gccgctgtcc aggaggatgt
     2281 cgctattgcg ctgttcctgc ccggggatcg ggtccatgat gaggatcggc ttccccaggg
     2341 ccagggcttc ggacgagcaa agacctcccg gcttcatgat gaccaggtcg ctggcgcagt
     2401 accagtcgat cagcccgttg acgaaacctt cgacccggac ccggggttcg tcctcgaaac
     2461 gggtccggag cgcctcggcg agctcctcgt tcctcccgca gacggcaaag acatccatgc
     2521 cgtcctgcct gacgagggat tccaccactc cctcgatggg ccccacgccg atgccgccgc
     2581 tgaccaccag gacggccggg gcgtccctct ccagcccgag ccttttccgg gcttcctccc
     2641 ggctcggggg agtgcagtag agcggatcga tcgggatccc cgaaaggacg acccggccca
     2701 ggtccaggcc gtcggccgcg tactgccgga cggcttcgtc gctggcgacg aaccagccgg
     2761 agaaggcccg gttccggtgg aagatgtggc tgagaaaatc ggtgttcacg taatagacgg
     2821 ggaccccggg gaaggcggac atgacggcgc tggtcccgaa gaagtgggtg aacaggatga
     2881 cgttgggggc aaagctctcg aggctgaggc gcagcttggt caggttcagc ttctccgcca
     2941 gctcgttcag ggtgctcagc aggccgtcct gggactcggg gtgctccatg gactcgtaga
     3001 aatagcccca gagctggggc gcacgcctca ccatctcgag gtagctccgg gtgtagatgc
     3061 cccgcaccag gggagaagaa taggccaggg tgtccaggca gacggtcttc acgccgggga
     3121 actcggcctg gaaccatttc gtgagggcca gggcggcggt cttgtgcccc gttcccacgg
     3181 acgcgtagag gacagcgatc cgaaactccc tcatggccct attcatccga tgcggagggc
     3241 tcccctgcac cctggagggg cacaggcttg cacaggtcgc gggatcttcg cgccacccgg
     3301 ccgcatttcc tgcagacgta aagaggttcc cgcacccatt ccttgtattc ctccaggttc
     3361 tttttcagga acccgggagt gatcctcttg cagaaaggct tttcctccga cacggcgact
     3421 cacccgtcct ttcgtctgga aaatccgtcc ctgctttcca tcatacccca gcgggatgcg
     3481 tttcaaaggc gtatacacct ttccgattcc catgcttttc ggccccattt ggcttgaccc
     3541 tgttttccct ttctttgcaa gggtcgagat gctttcttga cggaattccc cagagcccct
     3601 agaatgggct ttgatgcata atcgtataca actaggaggg ggtacgtttg aagaagaagg
     3661 tcaccgttct gggttgcggc ctggtggggt ccgtcatggc tctggacctg gccgccgacg
     3721 aggcgtacga ggtcaccgtc tgcgacgcca gcctggaaaa cctttccagg acgaaggcaa
     3781 gggcgaaggg acccctcgcg atccgcaccg atgtcgattt cgcctcgccc gcttccatca
     3841 cggaagccgt gcggggacag gatctcgtca tcggcgccgt tcccgggttt ttgggctacg
     3901 cgatgctcgg ggccgtcatc cgtgccgggg tcaacatgtc cgacatctcc ttcatggggg
     3961 aggattacag ggaatgggac gccgaggcga aaaaggcggg cgtcaccacc ttcgaggatg
     4021 tcggcgtggc ccccggggcc tccagcatcc tgatcggcca cgcctgcgag cagctggacc
     4081 gcgtcgacga cgtcacctac tccgtcaccg gccttcccac caggccggaa cccccgttca
     4141 actacaagct cgtcttttcc cccgacgacc tgatcgagga gtatgtccgt cccgcccgga
     4201 tgaagcgcga cggcaagatc gtcaccgtcc cggcgctttc cggctgccgg atcatgaagt
     4261 tcgacatccc cggcatcccg cttcccgaga tggagggatt cttcaccgac gggtgccgga
     4321 ccctgctgga cacgatcccc tcgccgaacg tgacggaata cacgctgcgc taccccggga
     4381 ccgcccgtga catgacgctg ctgcgggaga tcgggctgtt cggcacggaa ccggtggacg
     4441 tgaaggggac gaaagtggct ccccgcgacc tgttcggagc gttggcctac ccgaggatga
     4501 agctgggcga agaagaaaac gagttcacgt tcttccacgt cgaggtgacg ggcgagaagg
     4561 acgggaagcg cctgcagtac acctacacgc tctacgacga gaaggacatg gccacgggat
     4621 tcccttccat gagccgcacc accggattcc cgtgcgtcat cgtgggaagg cttctggccg
     4681 aaggcgtgct gaacatgccg ggcgtcaatc cgcccgaggc ggtggggaaa aaccccgagg
     4741 ccgtccggcg gctgctcgag gaactgcaga aacggggcgt gaagatcgct tcttcggtga
     4801 aggaactgta gaaggtcgag gggggaaggc gccgccttcc ccccttattt tttctttcta
     4861 cttcccgttg agcttttcct tcagcaggtc cccgagagaa agcccgacgg ggcggtcggc
     4921 ctcccgggcg gtcctcaggg cggaaagcag gctttcccgg atctggtcgg cggtgtagtg
     4981 gggcgggaag tacagcgccg cggcgtcccc gtggccgcct ccccggacct tccggccgtc
     5041 cttcagcagg ccggtgatcc ggccggccag cccgtcccgg ctcctcatcg acacggccca
     5101 gtcgccggcg ggcctccggg cgggaaccgc cacgagggcc ggggggttct ccatccggtc
     5161 caggacgagg ccgcaaaagt cggagacgtc cccgaactcc agcaggtccg gcggaacgta
     5221 gaccagctcg tcccggacgc gcaggacgcg ttcgagcgcc cgggcgaacc gttcctcctg
     5281 ggccctgacg aaccgctccg ccacttcccg ttcggactcc gtgagggcag cgtccgggtc
     5341 ggccgccagg cgcatcagga ctccccaggg accgcaaagg gtcaccatgg cctgccagag
     5401 cctgctgtcg ggctcctgcc cgagccagag gtcccggtcg ttggccatgt tcacgatggc
     5461 gcccatccgc tccacccgtt ggcggaccga ggcagaaagc ggctgttcca ggagccaggc
     5521 gtggtagacg gccgcggcgc agcgggagat gtccacaacg gcccagggcc ggttcccgta
     5581 gcggtcgaag gtggtttcat ggtgatcgaa gaggaaggga aggtcccccg ggacgtaccg
     5641 ggtatccagt tcgtccacgg tctgctgccg ctggcagaag aggtccagga ccaggggttt
     5701 ctggccggcc tgcaggcttt ccagcagcag gttgtccacc tccccgtagc ccgtcagcgt
     5761 gatcttcacg ggcgcggatt ccggccaggc ggctgcccag gcgacggctg ctgcggtgac
     5821 cccgtccagg tcggtgtggc tgatgatgtg ggtaacggtc ttttccacgc ggctctctcc
     5881 tcccgggaac ttttccacaa gattgtagcc taaaacgctt ccggaaaaac cttcggcttc
     5941 cgggcggatc cggggcattc ttttccttcc acaattgttt tccccatccc cccggcgttg
     6001 tgcatcgcgg atgtggataa tctttcggcc ttgctggaag aacttggtca ccgttcgtca
     6061 gattgtcgtc gttccgctga aaattttccg ctcccgcttg gatgaagggg ttttgcccat
     6121 tcacaaagaa cgtccctcag gatgtggata tgtgtataac ttttgtggat gttcttgtgc
     6181 tattcttttt ctgcacgctg gaagaaacgg catcgttgag gtgatctttt tgtccaagga
     6241 agcgcaggtt ctctggcagg acatcattcg ggcaacccgg gaggaatctc caggcggagc
     6301 ctgggaaatc tggatgcgta cctgcgagcc gctggaggtc gtggacggga ccctggtcct
     6361 cgacgttccc aacgttttcg tcaaagaaca gatccagtcc cgcttcctgg aggccctgaa
     6421 cgattccgcg cgcaagcgcg gggacctccg cggggtcgag ctccgcgtca gctccgaggt
     6481 caagccggcc gaacagcagc gggccgagaa ggcggcatcg cctcccccgg tgtcccgcca
     6541 cggtttgaac ccgaattacg tgttcgatac cttcgtcatc gggaaatcga atcgcctggc
     6601 gcacgcggcg agcctggcgg tggccgagtc tcccggcgtg gcctacaatc ccctgttcgt
     6661 ctggggcggc gtggggctgg ggaaaaccca cctcatgcat gccatcgggc attacctcct
     6721 gaacaaggat agcggcgcca aggtctgcta cgtcagctcc gaaaaattca ccaacgagct
     6781 gatcaccgcc atcaaggaca accggaccca cgaattcagg aaccggtacc gctccgtgga
     6841 cgtccttctc atcgacgaca tccagttttt ggcaaacaag gagagcacnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnac gaacatcccc aggaacatcc gcgagctgga
     7021 gggcagcctg aaccggatcg tggcctgtgc cgagctgaac aacgagccca tgacggtcga
     7081 gaacgcggcc tcctggctga aggacatcat tcggcacgtg gcgaagggac cggttacgat
     7141 cgacttgatc cagcagtacg tcgccgaggg cttcggcctt tcggtggaag acctgacttc
     7201 cagcaagcgg accgccgagg tttccctggc ccggcaggtg gccatgttcc tcagccggac
     7261 gcataccgag gcaagccttc agcagatcgg gtacgccttc aagaagaagg accataccac
     7321 ggtgatccac gcctgcaaaa aaatcgggga aatcttgaaa agcgacccga aaatcaaaaa
     7381 ttttgtggat aacatcacgg aaaagctgtg aagctgaccc gaaaaccggg gtacttatcc
     7441 acaaggattc actgagtaca ctttttccac cccgttcttg tggatactgt ggacactttg
     7501 tgggaggatc ttttcctttt ttccacaggc tttttccggc cgccgcaggc ctttggccgg
     7561 gttttccacg aatccaccgg acctagatct tcttctacgg gtttttttgg attacgggtg
     7621 caaaagacgc aggaggggag cgtgacaacg tgaagttgcg gatcgaaaag aacgcctttg
     7681 taaaaagctg gcagatggcc gagagaaaca ccagcacgac aagcactctc agcgtccttt
     7741 ccgggatccg ttgcctggcc agcggcagtt ccaccacgct tcaggcgacc gacctgaaga
     7801 ccagcgtgac ctgccgggcg gcgggcgtgt cggtggagga acccggggag gtcgtttttc
     7861 ccattaaagt ggtgggagag cttttcaaga aggtcccggc cgattctttc atcgtggagg
     7921 tgcgggaggg gaaggcaacg ctcctggcgg ggaaaagccg gtatcgtttc acgacgtacc
     7981 cggcggacga gttcccgaag ctcccggtgt cgacagcggc gcgcctgctg ttcgacacga
     8041 ccgtcaacga tctggagaga ctcctggacg aggggacctt ttcgggttct ccgaacgagg
     8101 aatttccgca gtacctggga acggcgctct tccaggtcgg gggaaaccgg gtgagggtcg
     8161 tttcaacgga cggaaggcgc ctttcgcttt ccatggcgga gatcccaggg aaggaaggcg
     8221 gtgaagaaac ccgggttctc ctccccctca aggggctgaa cgagttgaag agggtcctcg
     8281 gaacgatgga tgaccaggcg cccctgaggg tcctggtgga cgatgcgcag gcctatttcc
     8341 aggccgaagg ggtggagttt tccgttcgcc gggtggattc gtccttccct ccctacgaga
     8401 agatcctgac gaaggaccga acgacctgga tgacgatcga ccgcatgtcg ttcgtttcgg
     8461 ccctggaaag ggccgagctg gtggtgcggg atttcagccg gatgatcgtg atgaacctgg
     8521 ctcccggggg cagcctgaag atcaccgcca gggcacccga ggtggggacc gcttccgagg
     8581 aggtcgacgc ggacattgac ggggaaccgc ttcgcatcgc cttcaatgcc cggtacctga
     8641 tggaggggct gaaggcgctt cacgggtccc tggcacacct gtgcttcaac ggccccaacg
     8701 ggcagatgag cctttcccgt cccggcgagg aaaatttcct ctacgtcctg atgcccatca
     8761 cgctgcccga agaggagccg gtccccggag aggatgcgct gtagctggtt tcagaccttc
     8821 gagttccgga acctgaagcc ccagcgcctg gaatgggccc tggggcttaa tctgttgagg
     8881 ggagaaaacg gggccggcaa gacgaacctc ctggaagtgc tgcggctttt gtcgggttgg
     8941 ggacctttcc gcgggaggag cgcctcggag gctccgcgtt gggaaggcac cgccggaaga
     9001 gcggccctga aaggcggttt ttccggggaa gaagacctgg aaattgcggc gttcgtggga
     9061 agaagggtgg atctccgcag ggacgggaaa ccggtcggcg ccgcgacggt caggaaccgc
     9121 gtcccggctc tctcgttcac gccggaggat cttgccctgg tcgagggagc gccggcaatc
     9181 cggagacgtt tcctggacgg gctctgtgcg ctgctgctgc cgctgtatgc cttccgcctc
     9241 ctggaatacc ggaaagccct ccggcagaga gcggcggcgc tgaggcagaa ccggggggtg
     9301 ggagccaccg ccaaagtcct ggctccgctg gcctcctgga tctgggcatc ccgggctgcc
     9361 gcggtggacc ttctccggat gggcctttcc gaggtgagcg aactccttcc cgggccggta
     9421 gagatcgctt tcagcagggg agggagcgtc ggcctggaag acgggctgga ggattactgg
     9481 acctccctcg cccgccacgc gcacctggag gggcggattg gactcccggt cgtgggacct
     9541 caccgggacg acctgctcgt ggcaagcggc gggaagtccg ccctgcaggt ctttagccgg
     9601 gggcaccggc ggagggtggc ggtggcgctg atgatggcgg ccggctgggc ggtggagcgc
     9661 aaactgaggc gcgctccgct tttgctcctg gatgaagtgg ccgcggagct ggatgacacg
     9721 ggaaggtcgg tcctggtcga ggcgcttaag gcaagaggat ggcaggtgtt cgccgccacc
     9781 gcggaaagcg cttttgacgg gtggcctgga gcggtatggg tcgtttcgga aggatcggtt
     9841 cagcggaacg cctgaggggg aacggttcgg atgatggaac ggtatgaagt catcgtggtg
     9901 ggagccgggc acgcgggttg cgaggcggcc ctggcggccg cgaggatggg gtgttcgacc
     9961 ctgctcttga acctctatat cgacaacatg gcgctgatgg cctgcaaccc gtccatcggc
    10021 ggcccggcga aaggacacct ggtccgggag atcgatgcgc tggggggcga acaggcccgc
    10081 gccaccgacg cctccacgct gcacatccgg tggctcaaca cctccaaggg gcccgcggtc
    10141 cagacccttc gcgcccagtg cgacagccgg gcctaccact ccgcctacct gaacgccgtc
    10201 gagaggaccc cgaacctcga ccttttccag gacatcgtga cggagatcgt cgtttcaaag
    10261 ggaagggtca ggggagtgaa gacccggcta gggttgacgt tccccgggga gatcgtgatc
    10321 ctggcaaccg ggacctacct cggcgggagg gtccatgtcg gcctggtgaa tttcccctcc
    10381 ggtcccctgg ggcagatgcc ggcgacggaa ctgacgaaaa gcctcgtgaa ggccggtttt
    10441 gaagtggggc gcctgaaaac ggggacgacc ccccgcatcc acgcggcttc cgtggactgg
    10501 gcttccctcg agccccagga aagcgcttcc gagccgctcg cgttcagcca ttggagccag
    10561 ccgcggatcc acgtggggca cgcctgctac cagacccgga cgacgccccg aacgcacgag
    10621 atcatccggg gcgccctcga ccggtctccg cttttcaccg gggcgatcga gggtgtggga
    10681 ccgcggtact gcccttcgat cgaggaccgg gtggttcgct ttcccgaccg ggacagccac
    10741 atcgtctttc tcgaaccggt gggagggcag acggcagaaa tctacatgca gaacttctcc
    10801 acgagcctcc cgttcgacgt gcaggtcgag atggtccggt cgctcccggg ctgcgaaaac
    10861 gcccggttcc tgcgccccgg gtatgccatc gagtacgatt tccttccccc gacccagctt
    10921 ttcccctggc tggaaacgcg gatcgttcga ggtcttttct gcgccggcca gatcaacgga
    10981 acgtccgggt acgaagaagc ggcggcccag gggctgctgg cggggatcaa cgcggcccga
    11041 acgctgaggg gagaagagcc ggtcgtcctg gggcgaggcg aggcctacat cggggtcctc
    11101 gtggacgacc tggtcaccaa ggggacccag gagccctacc ggatgtttac cagccgctgc
    11161 gagcaccgcc tgatcctgcg ccatgacaac gcggaccgcc gcctggcccc gatcggccgg
    11221 aagctggggc tgatcgacga cgaccggtgg cgggtcctcg aagcgcgctg ggaggccctt
    11281 gaagcggaaa aggcacggct ggagaggacc aggatcttgc cgacggagga gaacaacgcc
    11341 ctcctgcggc aggcaggaac cgttcccatc gaaatgccgg ttccggccct ggaactgctc
    11401 aggcggccgg aagtgacgtg ggatctcctc caggcgatgg cggaaacccc ttcttccctt
    11461 tctgccgacg aggcggccac cctccagacg gagatcaagt acgccggcta tatcgaaagg
    11521 gagaaacgcg ccgtggagcg tctcgagcgc atcgagcggg tcgcgatccc ggaaggcttc
    11581 gattacgatt ccgtcacggg tctttcttcc gaaagccggg ggaaactttc caggtttcgc
    11641 ccgctgacac tggggcaggc ggggcgtatc tcgggcgtaa cgccggcgga cgtccagatt
    11701 ctctgggtgg cggtgaaagc ttaccatgag cgcgaatcca gctaggggta agcggcaggc
    11761 gaaagacctg agcccgatcg gggccttcct gggccgcggc ttgagccccc aggcgcggat
    11821 gcggctgaac ctggccgacc tgtcgaagaa ctgggagaag gtcgtcggcg aggtcctggc
    11881 caggaaaagc cggccggttg ccctggaaga cgggatcctg gtcgtggcct gcgagacgtc
    11941 ttccgtggcc caggaaatcc tggtgcggcg ggatctcatc gccgacaggg cccggaagga
    12001 atgggggctg gcccttaaag gggtgcgccc ttccgtccgg aggattcccc tcccccgaag
    12061 cgccgcggtg aaaaaggaat cgccgccccc gttcgaacct cctcgccatg aggtcgaaaa
    12121 ggccctggcc aggatcgggg gacggatcgg caacccggag atcgaaacgg ccctggcccg
    12181 gctcatggtg acgttccggc gccgcttcag ccggggaaaa gattgaaaag aagcaaagtt
    12241 atgagaaaat ggaccgggtt gaaaaaatcg atgccgagga gaaaaaaaga atgggttacc
    12301 ccacgttgaa aattggaaag catcagcccc gtttcccgct gatccagggg ggaatgggcg
    12361 tcaagatctc cgggccgcgc ctggccgggg ccgtggcgaa ggccggggga gtcggaacga
    12421 tcgcgacggt cgggctggcc gtcgactctc cccattacaa cggcaggaac tacttcgacg
    12481 ccaacgagct ggcggtgaag gaagccctgg ccgaagcccg ggccgaggcc cccgaagggg
    12541 tgcttgccgt caacgccatg gtggcactca ccgattacga gaggcacgtg cgggccgcct
    12601 gcgagggagg aacggacatc gtcatctccg gggccagcct gcccctgaag ctgccggaat
    12661 acgtgaaaga gttccccgac gtggcgctgg tccccatcgt cagttccacg aaggccgcca
    12721 acctgatcct ccgccgctgg gaaaagcagt acggccgaat cccggacggt ttcgtcgttg
    12781 agaccccgct ccacgccggg gggcacctgg gggccacgaa gctggagcag gtcaccgacc
    12841 cggccctggc gctggaggtg gtcgtgcccg aactggttgc ctttctccgg gacgaggtga
    12901 aggtcgacat tcccgtcatc gccgccgggg gcatctggga ccggtcggac atggaacggg
    12961 cctttgcctg cggcgccaag ggggtccaaa tggggacccg tttcgcctgc accgtggagg
    13021 gggacgcgtc cgaacgcttc aagcaggcgt acatcgatgc ccgggaagag gacgtcgtca
    13081 tcatcaaaag cccggtggga atccccggaa gggccctgaa gacccccttc gtggccaagt
    13141 acctggaggg ggatgtcacg agcaagccct gcatcgccaa ctgtctttcc cactgttcct
    13201 accggaacga ccgcagcgcc ttctgcattg cccaggcgct ggtggacgcg tacaggggcg
    13261 actacgaaaa cggcctgttc ttctgcggcg acaacgtggt gaaatgcacc cgcatcgaga
    13321 ccgtgcctga aatcatgaag gaactgttcg gggactgaag aaggagcttt cggggggaag
    13381 ccggaaaatc tccggcttcc cctttttttt gttcccgccg cacggtataa tgattaaaaa
    13441 cgctttagta aaagacaaga atgattccat tataagaaag gggctctgcg ggatgctgga
    13501 aatcccaacg ccggacaccc ttctcatcca ggatccgaag cacgtccagg cgttcctgct
    13561 ggaagtgcag gaaatcgcgc gcatgggcag ctggatctgg gagcccggca cggattgcct
    13621 cgtgttctcc tctccgctcg cttcgatcct gggtctcccc cgaagcggga ggaccaccat
    13681 ggaagaattc atggcgcgca tccatcccga ggaccgctcc cgggtggccc gggttcgttc
    13741 cgaagccctt tcctccggcg cggacggcta ctcggcggag taccgggtcc tgggaaagga
    13801 cggcatcgtg cacgtggtcg cgaggggccg cgtcgagagg aacgaaaggg gaaccccgtg
    13861 tcgggtctac ggctccatcc aggacatcac ggagcgcaag cggatggaag aagcgctgga
    13921 ggaggagcgg tcctacctgg ccggcctttt cgagcattcc cccgacgccg tcgtcgtgac
    13981 ggacgacgag ggaaggatcc agaaaaccaa ccgggctttt tcccgccttt tcggctacac
    14041 ggaagaagaa gccgccggac ggctgatcga cgacctcgtc gttccggaga ggctcttcga
    14101 ggaagggcgg ggatacacgg cagacctcag ccggggggcg cgcctggatt tcgagtctgt
    14161 ccggcgcaga aaggacggtt ccgagtttcc ttgccgcggc gtgggcgttc cggtcaggct
    14221 gagcggcggg cagctcgggg tctattgcat ttaccgggac ctgaccgcgg agagggcggc
    14281 ccgggaagat ctcaaaaggg ccctcgaaac catgaaaaaa gcctgggagc agaccatcga
    14341 ggccctggct tccgcctcgg aggtgaagga cccctacacg gccggccacc agaggcgggt
    14401 agcggtgctg gcttcggcca tcgcccgcga gatggggcat gcggacacct tcgtgacggg
    14461 tgtcgaaaag gcggccctgg tccatgacct gggcaagatc gagatcccgg cggagctcct
    14521 gagccgcccg ggccgtctat ccccctacga attccgcctc gtccaggtcc acgccgaggc
    14581 gggcttccgg atcctgagca agatccacct tccctggccg ctggccgaga tcgtctacca
    14641 gcaccacgag cgcctggacg gaagcggcta cccgagagga ctgaaagggg ccgaaatcct
    14701 tcccgaggcc cggattctcg ccgttgcgga tatcgtcgag gcgatgggct cccaccggcc
    14761 gtaccgcccg gctcgccccc tcgcggaggc cctggagacg atccaggagg gaagcggaaa
    14821 aaccctggat cccgaggcgg tcgccgcctg ccttcgcctt ttcctggaaa aggggttttc
    14881 gttccccgaa ccggcagcct gagcacgtat acaatattcc ctccccttct cgacgggttc
    14941 tttaaccgca agaaaagaca gccttaaagg ccgtttcccg cagcgcgccg ccagccgagt
    15001 tttccctgct ttccctaggt aaatcgacgt tgacaacatg aatccgtcca cgtatattca
    15061 taaaaatttt cgcgggttgg gacgattttt tcaggttctg tatgatattt cacaaggaac
    15121 ctgaaccttc ccgtcctcgt caatctctcg ttcagagagg caaagcggga aaaaccccaa
    15181 attccctttc tttccaaaag gaggctggcg gatgaagcgt gcgttcctgt tcttctggat
    15241 cctgctgggg aaggcccagc gcggcatcat gatcttcacc tccctggtcg tcgtcaccct
    15301 catcctcatc caggtggccc tgcgctactt cttcaagctg ccgctcatgg gagtcgaaga
    15361 gctggccacg atggtcggct tctggatgta cttcatcggg gcggcaaacg gctcccggga
    15421 gcggagccac atcaaggccg acatcctcaa cgccatgatc aagaacgaga aagccctctt
    15481 tttctccaag gctgccggaa acctggtcgt cacggtgctg gcctgcatca tggtccagtg
    15541 ggccatccgg tacttccagt ggtcccttag aagctgggaa cgttctcccg ccctcatgat
    15601 ccccatggtg tacgcccagt tcagcctggt ggtcagttcg ttcctcatgg ccttctactt
    15661 cctggtggag ttcctcgaca acgcccggca gttcttcggg aagcccccct tcgccctccc
    15721 ggacgacgat accggctttg cctgcctggc tgcccccggc gaacagaagg agggaaggta
    15781 gatgcatatc gtcatcgacc tcgtcatcct catttccacc atcctgctcg gcatccccgt
    15841 tcccttctgc ttcatggcgg ccgccctcta catgggcgtc attttcttcc ccgacttttc
    15901 cttcctgaca accgtgggat ttcgggggtt gaactcgctg acgctgctgg ccatcccgtt
    15961 cttcatcatg gccgggtccc tcatgggctc ggcgggaatc gccgagcggc tggtcggggt
    16021 cgccaactcc atgctgggcc ggatccgggg cggcatggga gccgcgacga tcgtggcctg
    16081 cgcgatcttc ggggccatct ccgggacctg ttcgtcggcc gtggcctgca tcgggagcat
    16141 catgatcccc cgcctggaga cgctgggcta cccgcgctcc tacagcacgg ccatggtcag
    16201 ctgcgccgcg gtcctgggcc agctgattcc tcccagcgtt cccatgatcc tctacgcctg
    16261 ggtgacgcag cagtccgtgg ccgcctgctt cctctccacg gtcatccccg ggctcctgac
    16321 gatgacggcc ttctgcatca tcaactactt catgtgcaag ggaatgccca cggtccgggt
    16381 ggatcccccg ctgccctccc gggagcggaa cgccctcgtg gcgaaggcct tcaagcgggg
    16441 tgtctggagc ctcctcatgc cggtcctcat cctgggcggc atctacgggg gcgtcacgac
    16501 cccgacggaa tcggcggcgg tggcggtggc ctactcgatt ttcgtgggct ttttgatcca
    16561 ccgggaactg aacgccagga tcctggggaa atccctctac acggcggcga ccacgagcgg
    16621 cgtcatcgtc ctgatgctgt tcttcgtcac catcctgggg cgcctctaca ccatgcagca
    16681 ggttcccatg aaaatcgccg cgttcctcct gagcttctcg aacaacaaga tcatgatcct
    16741 gctcatggtg aacgtgttcc tcatcatcat cggcatgctc atggacgacc tgagcggaac
    16801 gatgctctcg gccccgcttc tgatgccgct gatgctccag atcggcgtgc acccgatcca
    16861 cttcgccgcc atcctgggga cgaacctggg gctggggaac gtcaccccgc ccaccgcccc
    16921 gatcctctac ctgggcggac ggatcggaaa cgtgccgatc gaccagtaca tcaagccggc
    16981 cctgatcttc atgatcttcg gcaacatccc catcatcatc ctgacgacct acttcccggc
    17041 cctttccatg tggctgcctc agctgttgat gggggtcaaa taggagcaag gaaaaagtcg
    17101 gcgcggaacg tccgcaaaag gggggatctc cgtccggagg ggcatttttc ggcaacggca
    17161 cggctggcgc aaaatgaatt tcactggctt gtcaagaccg attcggaatg gagggagaga
    17221 atgaagatga aaaaatgggt tttcctgtct ctggcactgg tgctggccct ggggatcgtt
    17281 tccttcgcgg cccacacccc cgcccaggca gctccgcagt accactggcg cctggcccag
    17341 gtccacccgg tggacagcga cttcgacatc cgcgcgaaag ccttcgccaa gaaggtcttc
    17401 gaccgcacca acggccgcat ccagatcgat gtctattccg gcggcgtgct gggcgactgg
    17461 accgaaacct tcgaaatgct catgcggggc agcatcgaaa tgacgatggc ccaggccaac
    17521 gccaacttcg accccaagct gaacctggcc tactacttcc cctacctggt caccaacatg
    17581 aaagaggcca agaaggtcta cgggcagggc ggatgggctt acaagatcat ccaggacctc
    17641 tgggcggcgc acaacatcaa ggccctggcg gtcatccccg tgggcatgtc cggcgtgtcc
    17701 ctgaagtccc ttccgaagga ctacgcgaac ccctccgtcc cgaaggggat gaaggtccgc
    17761 gtcatgccga tcaagccctg cgagctgacc tatgaagcgc tgggctacat cgccaccccg
    17821 atcccctacg ccgaagccta cagcgcgatc cagacgggca tcgcggacgg ccagatggga
    17881 gggcccccgt accaggcctg gcagttcaag gacgtcaaca aggtgtggat ccagtacaac
    17941 gactacttcg aatcctggtg gttcgccatc aacatggatg tctggaagaa gctgtccaag
    18001 gctgaccagg acatcatcca gaaggccgcc aacgaagaat ccctgatccg gtgggagaac
    18061 gtggcgaagg aagacgaaga gtaccgccag aagctgaaga aggaatacgg ctggcagatc
    18121 gtcatgctga ccgatgcgca gctgaaggaa gttgcaaagc aggtccgcaa gaaggtttgg
    18181 ccggaaatgg agaagctggt gggcaaggag ctcatggacc gggtttacaa ggaatccggc
    18241 atcccgaggc agtaagcgcg aaggagatgc agccgcgggg cggggggcaa aagccccccg
    18301 ccttttttaa aaggagatga agaacttgac ccgaaacctg gagaaatgcc tgaaactcgt
    18361 ggaggaggac cgcgccatca cttccccggc ggcgaaggtc ccctactacc ctctcgtcgt
    18421 gaagaagggg cggggggcgg tgatcgaaga cgtggacggc aattgctaca tcgacctcct
    18481 ttccagcgcc ggggcgatca acacgggcca ctgccacccg cgggtggtgg aggccatcaa
    18541 gaggcagacg gaagacctga tcctctacac gcacgtctac atgtaccatg aaccgatcat
    18601 cgaactgacg aaagaactga tcgccatttc ccccggcgat ttccggaagc gcgtctatta
    18661 cgggctctgc gggtcggacg ccaacgacgg ggccatcaag atggcccgga cctacaagaa
    18721 gcgcccgaaa atcctctcct tcatccgggc ctaccacggg agcacctacg gggccctcag
    18781 cctcagcggg gtgagcctgc cgatgcaccg cgggctggga ccgcttctgc ccgagatcta
    18841 ccacgttccc tacccggatc cctaccgccc ccccgtgccg gggatgacgc cggaggaggt
    18901 ttccgactac tgcattgact acatccggac cgccttttcc aaccatatcc cggcggacga
    18961 ggtggccgcg gttttcatcg agccgatcca gggcgacgcg ggactggttg tcccgccggc
    19021 gaagttcctg aaggacctgc ggaagctgtg cgacgagaac ggcatcctgc tgttctcgga
    19081 ggaggtccag cagggcttcg gccgcaccgg gaaatggttc ggcatcgaga acttcgacgt
    19141 ggtgccggac gcgatcatcc tgggcaaggc catcgcctcg ggcctgcccc tcggcggctg
    19201 cgtggccagg gcggagctga tggacgactg ggaggctccc tcccacctgt tcacggcagc
    19261 cggaaacccg gtctgctgcg ctgcggccgt ggccacgatc aacgtcatcc gcgacgagaa
    19321 cctcctgcag cacacggcgg agctgggggc acacgtcaag gcccgcttcg aggagatgca
    19381 gaaaaagtac gaactgatcg gcgacgtccg ggggatcggg ctttccgtcg gcgtggacct
    19441 cgtgaaagac cgggaaacga aggagcgcca ccgggaagcg gcggccaaga tctgctaccg
    19501 ggcctgggag aaggggcttc tgctttcctt cttcagcggg tcggtcctgc ggatccagcc
    19561 ccccctcgtc atttcgaaag agcagatgga ctgtgccatg gaccggatcg aggaagccat
    19621 cgaggatttc gagaagggac gcattcccga ctccgtcctc gagacggtca agggctggta
    19681 ggaagagaaa gggagggatt ttcgtgaaag tgccgaaagg cagctgggtg cagatcgagc
    19741 aggtggtcct caagccggaa gagagggccc cgcaggtccc ggaggacacg aaggccgttc
    19801 ccctcatgct ctgggtgaag gggacgcttc ttgaagacgc ttccctcggc gaaaccgtgg
    19861 agatcgaaac ggcgatcggc cgaaggctta ccggcaagct cgtggcggag aacccgccct
    19921 acacccacgg cttcggccgg ccggtcccgg agctcctgtc cgtggggaag gaattccggg
    19981 agctcatcga aaggagtggc cgcagatgag ggacaattcc tacgcggcgg tcatggcccg
    20041 gcgcaacgag atcatgaaac gggccctggg cctggactac ggggaatttg agctgggggg
    20101 cctggccttc gactacgagc ggatgatgga gaaggccggg tattccctgg aagaggtccg
    20161 ggcgatccag aaagaaacga acgtggggga tacgcccctc atcgagctga agaacctcac
    20221 ggcggcggcc cgggccctct cggagcccgg caagggggcc cgcatcttca tcaaggacga
    20281 ggccgccaac ccctccggga gcttcaagga ccgccgggcg gccgtctcct gctacttcgc
    20341 ccagcaccac gggtacaagg gcgtcgtcgc cgccacgagc ggcaactacg gggccgccgt
    20401 ggcctcccag gccaacatga gaaacctcaa gagcatcatc gtccaggagc ttttcgacag
    20461 ccggggaatc ggccagccgg agatcctgga aaagggaagg aaatgcgagg cctacggggc
    20521 ggaagtcctt cagctcaccg tggggccgga gctgttctac accttcctgc gggtgctgga
    20581 ggacacgggc ttcttcaacg cgtccctcta cacgcccttc ggcatcgcgg ggatcgagac
    20641 gctgggggcc gagatcgtgg cccaggtgca ggcccgtttc gggcgcaacc ccgacgcggt
    20701 ggtcatcacc aacgcgggag ggggcaacct gaccgggacg gcccgcgggc tgatcaaggc
    20761 gggggcggcg gaaacgaagg tgatcggggc ttccgtggat ctctccggtc tccacatggc
    20821 cagcgaccgg gacttcaacc gaaaatcctt caccacgggc cataccggct tcggcgttcc
    20881 cttcaccacc tggccggacc gggcggacgt cccccggaac gccgcacgtc cgctgcgcta
    20941 catggaccgc tacgtgacga tcacccaggg agaggtcttt gccatcaccg aggccctggc
    21001 ggccctggaa gggctggagc ggggaccggc cgggaacacc tccctgacgg cggcgttcgc
    21061 catcgcgcgg gaaatgccgg aagacgccat catcgtcgtg caggaaacgg aatacacggg
    21121 agcgggcaag cacgtcaccg ctcagctcac cttcgcccgc gagaacggca tcgaggtccg
    21181 gcggggggac ccggccgacc aggtcccggg gaagaacatc atcatcccca gcgacccgtc
    21241 gatgatccgg cccagggatc tctcgatgag agacctgaag gcgtcgtacc tgagaaacgc
    21301 gctgaagaac cgccctgcgg gcagcccgga ggccctgacg gaaacggaag ccgccttcct
    21361 ggcagcggag atcaaggaaa cgccggaagg gctggcggcc ctgctggccc aggcccgggg
    21421 ctgctgaggc ccgaagggaa gacagggggg cggacgaaag gtccgcccct ttttcgtttt
    21481 tcgaggctcg cccctatact ggagaggtcc gaatcctttc aggaggaaaa aacgatgatc
    21541 gagaagagat tgctggaaga aaccctggcg ctggccgttt cctacctcga ggggttgccg
    21601 gaaaggcccg tgggggctca cgactcggtc gaagccctga gagcccgcct aaaggtcccg
    21661 ctcccggaga ggggaaccga ccccgaaacc gtcgtccggg agctggccag ggacgccgac
    21721 gggggcctgg tggccagcgc gggcccgcgc tacttcggct tcgtcaccgg gggatccctt
    21781 ccggccgccc tggcggccga ctggctggcc tccgcctggg accagaacgc cgtgctctcc
    21841 atcgcctctc ccgtggcagc ggtcgtcgag gaggccgtcg cggactgggt gctggaactg
    21901 ctggacctgc cgcggcaatc gtcggtgggt ttcgtcaccg gctgccagat ggcgaacttc
    21961 accgccctgg cggccgcgcg gcacgaggtg ctgcggcagg ccgggtggga cgtggaagcc
    22021 caggggctgt tcggagcccc gccggttacg gttctggccg gggaagaggc ccacgcgacg
    22081 cttttcgtcg ccgtgaggat gctgggcctg ggggccgacc gggtgatccg ggtggcggcg
    22141 gacgggcagg ggcgaatgga cccggcggcc ctcgagaggg cgctgaacgg cgtgaaggag
    22201 cccctcatcg tctgcctgca ggcgggcaac gtcaacaccg gcgctttcga cccctgcgcg
    22261 aggctgatcc ccatggcccg cgagcacggg gcctgggtcc acgtggacgg ggccttcggc
    22321 ctctgggggc gcctgcttcc cgagctggcg gccgagacgg agggcatcga ggcggccgac
    22381 tcctgggcca cggacgccca caagtggctg aacgtcccct acgactccgg catcgccatc
    22441 gtgaaaaacg aggccgccca ccgggcgtcc atggtgctgg cggcgcccta ctacatcgcg
    22501 gccccggacg agatccggga tccttcccag tgggttcccg aatcctcccg caaggccagg
    22561 ggctttcccc tctacgccgc gatccggtcc ctgggccggg agggactggc cgaaatggtt
    22621 catcgtcact gccggcaggc ccggctgatg gcggacctcc tccgggagga cccccggatc
    22681 gagatcgaga acgaggtcgt cctgaaccag gttctcgtcc gcttccttcc cgaaaacggg
    22741 gagggggatg tcgacgcctt caaccggaag gtcacggccc tcgtgcagca ggaaggcacc
    22801 tgctgggccg gggggagcac ctggaagggg aaggccgcca tgcggatttc cgtgtcgggc
    22861 tggccgacga ccgacgagga tatccgcgtc tccgcggcgg cgatccggaa ggtgctggcc
    22921 cgcaccccga ggtgaacaga aggggcgata cttgacgggg tattctaaag cttggggata
    22981 tcgccaagaa ttgtggaagg aggggtttcc ctggacggtt cgacgacggc ggtgctttac
    23041 gggctggcgg cgctcggcct ggccctttca tggcggcgcg accgggacaa gacccgggcg
    23101 gcgcttgcca aggcaagggc gagcctgctg atgatcctgt cccccctggt gacggtcatc
    23161 ctgctcgtgg ccctggtcct gaccgcgctt cccccgtcgt tcctctcccg cctggtcggg
    23221 gcgcaaagcg gattcccggg agtcctcctg gcctcgctgg cgggctcggt gaccctcatc
    23281 ccgggctttg cggcattccc catggcgaag atgctcctcg acaacggagc cgggatcgtc
    23341 caggtggggg tgctggtttc caccctgatg atggtggggg tggtgacact gccgatggaa
    23401 cggtccattt tcgggtggaa ggcgacgttg gcacggaacg cactcgccta cggacactcc
    23461 ttcctggtgg gcctggcgct ctggatggcg ttccggtagg aggcggggaa tgaaacggtt
    23521 tcccggcatc ctggcggcaa cggctatctg cgggttctac ctggcggcgt tccctgccca
    23581 ggcccgcggg tttttctccc tggcggggtc caatttcctc gtcgtgctct cgatccttcc
    23641 tcccgtcttc atccttttgg ggctctttga cgcctgggtg ccgagggaac gggtcacggc
    23701 cctgatcggg gaaaaatccg ggctcgtcgg cgcggggctg tcgatcttcc tgggatcctt
    23761 cgcggcgggc ccctgtacct ggcctttccc atcgcggaaa cgctgctgaa gaaaggaacc
    23821 ggcctgttca acgtgttcct tttcgtcggg gcctggtcga cgatgaagct tcccatgctg
    23881 atgttcgaga tgcagagcct ggggctgcgc tttgccctgg tgcgctacgc gatgtccttc
    23941 ctgggcatct tctggatcgc ctgggccgtc gagcggcttc tcggggaaag cggccgacgg
    24001 gaactcttgg aacgtttccg gccttgacag ggccgaggcc gttaaagttg cgggagtggg
    24061 aaggggttgc ccgaaaggca agaagccggc agagcggtca aagaggcttc aaaaagggaa
    24121 tattaaccgc cgaccctcgg ccctggcctg gaccaaaaag gaaactcctg catccgggag
    24181 cgtaatggaa cgggaagatg tccttgttct cgaggccttt ttttgctaga gtcagatcca
    24241 tagacattct aggcatataa ggggtggggc gaatggccga tctcacggga gacaaatatt
    24301 gggatggaat gataaattca gccctttgca agctttttgt cctaagcgcc ctgcaggaaa
    24361 ctcccatgca cggctatgct ctaatccaaa agattgcctc gatgaccgat gggttttgcg
    24421 tgccgaccga gggggggctt tacccggtcc tgaaagagtt cgagagctgc gggtgcgcga
    24481 ccgtatcgtc cgaagtggtg gaaggccgga cccgcaaggt ctatgccctc accgaaaagg
    24541 gaaggaggtc tctccaggca ggcctggatg cctgggaaaa ggcgattccg cacctggtcc
    24601 gtgcgcttcg gcgctcggga aaatgaacga aatcgatcaa acggggggaa gggaaaagtg
    24661 aacgatcttg ccacgtcggc ccggttcttt ttcttcatcc tggcggaact gaccgtgctt
    24721 ttcatcggca tcgacctctt ggtcaaccta ctccgcgaat acgtcccgga cgagcggatc
    24781 cagaaactca tgggccgcaa agggctatca ggctacgttc tgggtgcggg gttgggggcc
    24841 atcacgcctt tctgctcctg ttccacgatc cccgtgacgg tggggctcct gaaagcgaag
    24901 gcccctttcg ggggcgtgat gtcgttcctt ttggcctcgc cggtgctcaa ccccattatc
    24961 ctgggcatgt tcctcttcct tctgggctgg aaggcctgcc tggcctacgg tctcgccgcc
    25021 ttttccgcgg cggtcctggc cgggttcgcc ttccagaaat ccgggttgga gaaggacatc
    25081 aagccgctgg gtgcggcccg ctccggatgt tgctgcgggg gcggcttcgt catgccccgg
    25141 ctgaccttct cgcagaaact ggcctggtcc ctgaagggag cattggaaga tttccgcggt
    25201 gtgctcgtct acatggtcat cggcgtggcg gtcggggcgg cgatttacgg gaccgttccg
    25261 gcctcgttcg tcgcctccgt cgcggggcca ggaaacccgc tggcgatccc cgcggcggcc
    25321 ctggtgggaa ttccccttta cgttcgggcc gaaacgatga tccccatcgc ggtggccctc
    25381 tcggggaagg gaatgggcat gggggccgtc atcgccctga tcattggcgg tgcgggcatg
    25441 agcatcccgg agatgggcat gctggcaggc atttttcggc ttcgcctcgt ggcagccttc
    25501 gtggccttcg tgttcctgac ggccgtgggc gccgggatcg ttttcaccct gatcgcctag
    25561 aaagattgaa gctgaacgcc tgctggggcc tgcgacagcc ttgtggcagc caggaacaca
    25621 caaatacttg acggggtatg ttatgagtgg tattcttgcc acgtgtaaag ggcgggaaac
    25681 ggttgttctt tacggcaagt cgaagcgggc tgcggtggtt acgcgctaat ggagggggca
    25741 gattgttgag agcgacgaag gaccgcaggg cggaaatcct gaaagcgctg gctcacccgg
    25801 tgcggctggg cctggtggag acgttgtccg accgtcgcct ttgcgtcaac gacctggcag
    25861 ggcttttccc ggtcgaccgg acgacgatca gcaagcacct tgcggtcctg aaggatgccg
    25921 gcgtcctgga agacgagcgg tccggcaggg aagtgtacta ccgccttcga ctcccgtgcc
    25981 tgcaggaatt catcgcctgc ctggggcgca tgtcgaacgg gggcgagcgt gaagtgtccc
    26041 tttgctgtgc gggccaagga aagaaagagc ccgctgcctt ggaaagaaag gagcaagaag
    26101 gatgaaaatc cagatcctgg gtacaggttg cccgaaatgc aagaaactgg cggagttggc
    26161 ggaggaatcg gcgaaggagc tggggctcga tttcgaaatc gaaaaagtga cggacatcat
    26221 gaaaatcctc gatttcggag tgatgtcgac gccgggactg gccgtggacg gaaaggtcct
    26281 ggcttcaggg aacgtgccct cgccggagca ggtgaagcgc ctcctgctgc aggccgctgg
    26341 aaagtagctc tccccccgct tcgtgcgggg tttttttccg cttgttatgt gccattattt
    26401 gcacttgtct tccggaagag gtgattttct tgaacggcga gataaagaag ttcctgtccc
    26461 tggcagctgt gtttcttgtt ttttactatt tgccggtgga cagcgcccgg atccagggcg
    26521 cggccatcga agcgctggcc atgatgcacg agtatgcccg gcagcacgtc ctgctctgcc
    26581 tggtgcccgc cttcttcatc gcgggggcca tcagcgtgtt cgtctcccag caggccgtca
    26641 tgcgctacct cggggccagc gcgaagcggt gggtggccta cacggtggcc gcggtttcgg
    26701 ggacgatcct ggcggtctgt tcctgcacgg tgctgcccct gttcgccggg atctacaagc
    26761 gcggggcggg cctgggcccg gcatcggcgt tcctctactc ggggccggcc atcaacgtgc
    26821 tggcgatcat cctgacggcc cgggtcctgg gcctcgagct gggcgtggcg cgggccgtgg
    26881 gggcgatcct cttcagcgtg gtcatcggcg ggctcatggc cttcattttc cgccacgagg
    26941 aagcggaacg gcagcaggcg ttcgcggagc ttcctgtccc ggaagcggtc cgccccctct
    27001 ggaaaacggt ctttttcatg gcgagcatgg tggttttcct cgtttttgcc aactgggcgg
    27061 cgccgaaggt gcccgtgggt ttctgggcgg cggcttaccg ggtgaaatgg atcgtggcgg
    27121 ggcttgcgct tctttcgacg atcgtttcgg cccggatgtg gttcgaccgg gaagagcgag
    27181 tcgaatggta cggctccacc tggggatatg ccctgcaggt gctgcccctg ctctttggag
    27241 gagtcctggt ggccgggttc ctgctgggcc gccccggcca cgaggctttg atcccgagtg
    27301 cctgggtttc ggccttggtc ggagggaatt ccttgttcgc caacttcttc gccgccatcg
    27361 tggccgcttt catgtacttc gcgaccctga cggaggtccc catcctgcag gggctcatcg
    27421 gggccggcat gggcaagggc ccagcgctgg cgctgctttt ggccgggcct gccctttccc
    27481 tgcccaacat gctggtcatc cggagcatcg tgggaacgaa gaaaacggtg gtctacgtgg
    27541 gcctggtggt gatcctttcc acggccgccg ggatgctgtt cggggctttc ttcggatagg
    27601 ccgggcatcc gtgatttgtc aaaagggagg ggttcgatga tgttcaagca tatcctggtg
    27661 gcaaccgatc tcaggcccag gtccggggaa atcctctgcg gactgggtgc catggatccc
    27721 gggctggttg ggaaggtcac gctccttcac gtgttggacg aacgctttaa cgggcgtctc
    27781 ctggcttctg agaaacagga gcagctggcg atgcagaagg cccgccttga agggcgcggg
    27841 ttcacggtgg aggccgaaac caaaatcggc gttccgttcg acctgatcgt tcacgaggcc
    27901 caggttcgcg aagcgagcct ggtggtgatc ggacgggggg acagccctcg ctggaagtcc
    27961 cgcatcatgg gagagacggc cctgcgggtc ctggaactgt ctccctgccc ggtgctggtc
    28021 tgccacggcg agggagccaa gaatcccttg ttcggggacg tcgtcctggg gatcgatttt
    28081 tccaacgagg cccatcacgc cttgcaggcc ataaagaagc tggcccggga gaatcccggc
    28141 gccgtgaaaa aggtcacgct tctccatgtc cacgagcaga aaaacatcga cttgctgctg
    28201 aagctggtca accgggaaca gatcgagcag atcgtcgccc tggagcagga gcggctggag
    28261 gaattcgcct tgagcctgaa agaggcagga atcccggacg tttccgtccg gatgcggacg
    28321 ggaaagcccg tggacgaaat cctggccgat atcgaggaaa acaggcccac cctcgccgtt
    28381 ctcggggccc aggggcaggg ccgatcggag atgtaccgca tcggcacgac ggccttccgg
    28441 gtcgcccaga tggcctcctg cggggtgctc gtcattccct tgtcgcgtcc ggccctgccg
    28501 ttctaaacag ggttaggaag agaaaaaaga ggagtcaaag aggaatccat gacggcggaa
    28561 aagaacgaca ccgaccacgg gcacgaccat ggacattccc atgttgcgac cggcacgtcg
    28621 gaggggcgcc tcgccctgtc ccttgttctg aacctggtca tcaccctggc cgaggtggca
    28681 gggggcttgt tttcgaacag cctggccctg ttgtccgatg cggtgcacaa cctgtccgac
    28741 gcctcgtccc tgggggttag ctggctggcg aggcgcatct cccggatgga gcggacaccc
    28801 agtcattctt tcgggttcaa gcgggcggaa gtcttggcct cgctggtaaa cacggtggcc
    28861 ctgatgggca tcggcttctt cctgctggtg gaggccgtcc gcaagttcct ccacccggaa
    28921 ccggtggccg gcggaatcat gctggccgtc gccgtggtgg ggctgctggg gaacctgttc
    28981 acggcctggc tgctccacgc ggactcgaag gacagcctca atatccggtc ggcgtacctc
    29041 catatcgtca tggatgccct ctcgtccgtg ggcgtgatcg ttgccgccct gctgatgatg
    29101 gccttcgggt ggttctggct ggaccccctg ctgacgctgg ccgtttctct ctacgtcctg
    29161 cgggaatcct ggcccctgct caaaacgtcc attcacatcc tcatgcaggg gaccccggaa
    29221 gggatccgga tcgactcgct ggtcgaggaa gtggagtccc agcctgaggt cgtagacatg
    29281 caccacgtgc acctgtggac cacggacggc acggaggtgt tcctggaagc ccacgtgacg
    29341 gtctgcgagg aagcccgaag ccgcacggac gagcttttgg cgaggctttc gaagcggatc
    29401 cgggaggagt tcggcatcgg ccacgtgacc cttcagcttg agttctccgc ctgcgtgggg
    29461 gagcgctgct tcggactgga cgggaaaaag ctgaatgcaa aaaaatagcc cggtcaggcg
    29521 ggaccctcgg tccctgaccg ggctgaccag ggttgcagaa gaagccttgg cgcggtttca
    29581 gtagctcact tccggggcga ttttgacctg caggaccgcc cccgctcgca gaacggtaaa
    29641 gaggttttcc cctcgaacga gaaggggagc cagcccttcg gggccttgaa ccggtttgcc
    29701 gttgcattcc agcaggatgt ccccgacctt gagctttgcc gcctctgcca cgcctcccgg
    29761 ggccagggaa gaaatgacgg cccccggttt ccctttcatg gccgcaagct cctcgttgtt
    29821 cggcaggcgg agggaaaagc ccagcttgat tggcccttga gaagtcgcgg ggcctgctgc
    29881 aggggttgag gaagcaaagg ttaacgtcgg cggaaccctg ggaaccttct cctccgggaa
    29941 cggttcgaca aaccgggaaa cggttccgtc cttcttcaac accttgaaat tataggaagg
    30001 gaattttccc cagaggtccc gaaagaactg ttcggcgttc tcgaccggcg tgtcgttggc
    30061 ttcgacgatc acgtcccctt cctggaggcc gccggccgca gcggggctgc cctgggagat
    30121 ccacgtcacc ttcatgcctt tcggttcctt caatcccagt tccttgaagg gctttgtctt
    30181 gaggtccttt tccgtgagcg tttcgaattt caccccgagc ctgggcattt ccaatttcgc
    30241 ccccgcggcg acggacaggg acaggagggc gttgaagagc gtctcggcgt aggcgcgggt
    30301 gggagtccgg aacgttttga tgtcgaaccc gcccggggat tccaccagca tgctcacgcc
    30361 ccacttgtat tccttgtcga ggtctggata ttgcatcagc cgcatggttt tgaccttgtc
    30421 cagctggacg acggtggttt cctcttcatg gaccggttcc acgaccggct ggtaatagcc
    30481 gccggtcagc cccagccccg tatagaccca ctgctgcctc gtgttggtgt agtccagcac
    30541 cccgaaggcc cggagcccgc ttcggtcgat ctcgatccgc tggaggggaa gtccttccca
    30601 gccgacgaac ttgtcctgca tgttccggat ggcgccctcg gcttcctgga cggttgaata
    30661 aaaggcagga acgtgccagt gttcgatcgc ggtcgcctcc gccgccccgg aaggccaaag
    30721 gagcagccag aggcccgaga tcaaaaggca ggaaagaaaa agaagccgaa gttcccgaat
    30781 tcgcatgacg atccctcccc gtttcgttcc tttctggatt gtcccgtttc taaagaagta
    30841 taaaaagaac ggaaggcctg gaaaagcgga cggagagacc ccattgaaag aagcctcgct
    30901 gtccgtcaaa agccctggta gaagaggaca aacgacagca aaagaagccc ggctcccatc
    30961 agcagcttca ggacggtgtt gaaacggccc atccgctcgc ttgaggatag cctggccgcg
    31021 aagctcgtga aggttcctgc caaaagaacg agagtgcaat gtcccacgga ataaagaagc
    31081 aggaggaaga cgcctctccc gacgtttccg cttttggcca cgagtgccag cagaacgacc
    31141 agcacgggcg tggaacacgg agaagagaag aagccgccca gaacgcctgc aagaaaggcc
    31201 ccgatgaccc ctttgggcag gttcgtggaa agggcattct ttgaggggac gaactcgaag
    31261 aggccaagga cctgcagggc catcatcagc atgagaaccg ccaggcccag ggtccaccag
    31321 ggggaagagg tcccaatcag ccggccgaac agagacgcgg cggcccctag cgcggtgaaa
    31381 gtcgaggcca ttccgacgga aaaggccagg gagaggacga atcctcgccg ggggtcactc
    31441 tggcgcgtgc cgcccacgta ggcgatgaca agggggatgc tggtcagggc gcacggcgta
    31501 accgacgtga tcactcctgc caaaaacgcc aaaagcggcg cgatggggga gccgctttgg
    31561 ataaagcccg aaagagcggc cagccaccct tcaatcaagg cggaccccca tctccgccag
    31621 cacggcgagc agctgttccc gggtcatgcc gccttcgtgg gcggtgagcg tgtgctgctt
    31681 tgtttccttg tccgagtaga ggatcatccc aaaccgcggg tcagaaggga caaagggttt
    31741 cccctcccgg tcgaaaaaga actgggtcgg gatgaccctc agggggtacc cttccgccag
    31801 tttctggtat ttccagacgt ccacgaactt gatgaccgct ttgccccgga gggtccggct
    31861 cagttcttcc aggacggggg ccatctcccg gcagggggcg caggagtccg ctccgaaatc
    31921 gatgaccacg ggcaaccccg aggcccgggc ctgggtcagc tcttctgcag aggtcacatg
    31981 cagggccgca aaatccgcct tcctcgcttt ttccctctgt ttggcgaacc agatgccgac
    32041 gatcgccagg gcaatgagca gaagggccgc gattttaacc gctgcaggtt tttgcaaatt
    32101 cttgacgttc atggctctcc gcctccatcc tctgccggaa attgtacacc ctccaggcag
    32161 ggcaaaaggg catcctgcct ccatcctggg aaggggagag tcttttgcct ggagaaatcg
    32221 gaacctcgag gttccctgtc cggttcagac cagccggaag cgcaagcgcc cggctccttc
    32281 cacggagaaa aggtaatcgc cctccggcgt cggggtggtt ttttccatcc actccagcac
    32341 gtcgtttttg agccagcatt tcagctgcgg atcgtcttcc acctcgtaaa gaacgtattg
    32401 atcgatctct tccggtgccg gttttcccca aaggccctcc gggcctttat aggccattca
    32461 tcacccggcg atgatcgtgt tcattcg
//
LOCUS       DAOOVN010000010        32265 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_918_length_32265_cov_16.358646, whole genome shotgun sequence.
ACCESSION   DAOOVN010000010 DAOOVN010000000
VERSION     DAOOVN010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 32265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32265
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_918_length_32265_cov_16.358646"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..1367
                     /locus_tag="PLY83_02240"
     CDS             102..1367
                     /locus_tag="PLY83_02240"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOV28886.1"
                     /translation="MPRSGGEPLPGTGPFRALRYRNYRLFFAGQAISLIGTWMQRVAM
                     SWLVYRLTLSPFALGLVDFASQVATLLLGFIAGAVVDRTDLRRLLVWTQGLAMGQALV
                     LSALTLSGKATFTVVLVCGALLGMINAFDMPARHSFVIRMVERKEDLGNAIALNSSLF
                     NLARLLGPAVAGFVIAAVGEGLCFLLNGLSYSSIIIALNAMHLAPASSERPQEAALAG
                     IREGFSYAWIRRPIRDTLILLSCLSLLGLSYVVMMPVFAKEILKGGPRTLGFLMGATG
                     AGALAGSMLLAVRKGTKGLEKGIPIASGLFSAAVIGFAFSPSAGASYVLVALAGFGMI
                     STLASCNTLLQTVVDNALRGRVMSLYTMALVGIAPFGSLLAGWLTDRIGVRLALALGG
                     VCCMAASVWFSRRVPLFNKPLFPEGERPF"
     gene            1375..3273
                     /locus_tag="PLY83_02245"
     CDS             1375..3273
                     /locus_tag="PLY83_02245"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOV28887.1"
                     /translation="MILLKIAVFLKQVPDTDEVRMDEKTGTLIREGVGTVINPLDQNA
                     LELALSLRAAKGGSVTVLSMGPAQAELALREALALGADEAVLLCDRAFAGSDTWATAR
                     TLAAAVRKTGPYDLLLAGAKATDGETGQVGPESASMMGLPFVSHVCAVEIQAECLVAS
                     RSVEDGTERIRAPLPCLLTVVHEINEPSMPTLAGKMRARRAPVRVLSAADLGLNPAEC
                     GLAGSPTRVVRIAFPKVTRSGLRFSGSELDRGIALVAERLDSMGLLGQKRPARSVDSA
                     RSPEAARPIPPVELAWEEAFKNDPVGGVLVCGECRNGSIHPSTLELAGKARELADSRG
                     SSVTVVLPFGHLDDDPARCIRHGADRVLAVCHPALGLYNPDVESRLFATLIRRLRPEI
                     VLAPATTSGRTTLPAVAALVDTGLTADCTGLSIDPENGDLLQTRPAIGGNVLATIRTP
                     SRRPQMATVRPRTFRLPPEDPSRRGEVYRLTPPEHFLESSLVPLGRETRQEEGAPIQD
                     AEVVVSGGKGLKRPDGFRLVEELAAVLDGAVGASRPTVEAKWIGYPHQVGLSGKVVTP
                     RVYIAVGISGAIQHLAGMQTSEFIVAINRDPEAGIFRVADIGLVGDLFDIVPRMAGAI
                     KARKEAPK"
     gene            3270..4724
                     /locus_tag="PLY83_02250"
     CDS             3270..4724
                     /locus_tag="PLY83_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOV28888.1"
                     /translation="MKEPSRFGTVTPEVVERLSAILGTGGVSVDGEKIAAYSRDEVAE
                     RFWDRPYRAEVLVFPESTGHVSEILRLANERRIPVTPRGAGTGLSGGAVPAFGGILVS
                     FERMNRILELDTENLCMTVEPGVITAEITRAARKAGLLYAGDPCSGDASFIGGNVAEN
                     AGGNKVIKYGPTGSSVLALEVVLADGSVTWFGGKRRKDVTGYDFVHLMVGSEGTLGII
                     TKIVLKLLPLPAFGVDLLIPFPTVEAAIRFVPRIALEGRVTPASIELVDHHSLSQAEK
                     FLNTRLPFDQAAAHLIVQLEGNDREVVADETERVGKLALEKGALEVFVADNRSSRDKL
                     WKARKSVAEAIMAYYQNYAKEDLVVPTDRVPDLLFAVDALCRKWRMEAANYGHVGDGN
                     IHVNLLMADDRPHWHEDMEQARQELYETVRQLGGTLSGEHGIGLKRKAYLPQFLDAPQ
                     LELMRRVKLAFDPNGILNPGKLVFWDKKTEEAGA"
     gene            4721..5443
                     /locus_tag="PLY83_02255"
     CDS             4721..5443
                     /locus_tag="PLY83_02255"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOV28889.1"
                     /translation="MRPRIGITCGFAGERDPLALDRDYADAVDRAGGVPLVVPPGVPG
                     EALAETLEALDGLLFSGGPDVDPARFGEEPLPGMGRIDPARDEAELFLARLGLEREMP
                     LLGICRGAQVLNIASGGDLYQDLPSQVEGVLKHVQQPTPMSYPTHRIRIEPGSLLARI
                     TGKVSLRVNSWHHQAIRRVAFGFIPTARAEDGVIEAIEKTGEPFVLGVQFHPESMGRG
                     GSPEAILLFEAFVQACARWARD"
     gene            complement(5440..6378)
                     /locus_tag="PLY83_02260"
     CDS             complement(5440..6378)
                     /locus_tag="PLY83_02260"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOV28890.1"
                     /translation="MRIKPKALRPGDRIAIVAPGSAAPEEKLKRGADSLAKLGFEPVF
                     GKTCFLKRGFMAGPDEARLADLHWAFSDPSIHGIVCLRGGNGSGRLLPGLDMGLIAAN
                     PKPFVGYSDITVFHTVFGQQGNFVTFHGPMATIEFSSKGEKGITRESFLKALTSTEPL
                     GVLADASSGLTSLAGGKARGEIVGGNLALICSTMGTPYELDTRGKILFIEDINEQPYS
                     VDRMLTHLRNAGKLSAAAAVVIGDFLNADPVSPGPEDSLTLDEVFEDHLPGLGIPVVR
                     GLRCGHGKLNLTLPLGIQVEVDANACRLTALEAALS"
     gene            complement(6383..6802)
                     /locus_tag="PLY83_02265"
     CDS             complement(6383..6802)
                     /locus_tag="PLY83_02265"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOV28891.1"
                     /translation="MEGILRTLSPEARRLARERIETVASRAAEALGARAEVRFQEGYP
                     CLENDPSMAALVRETGCLLLGEENVLEVPRPSMGVENFSFFARERKAAFFTLGAGNPA
                     KGIVHPAHSSRFDLDEDSLPIGAAMLAGVALNFLQGR"
     gene            complement(6709..7578)
                     /locus_tag="PLY83_02270"
     CDS             complement(6709..7578)
                     /locus_tag="PLY83_02270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOV28892.1"
                     /translation="MTGRRFLDGLLEEAIGLHEDLVRLRRSIHRRPEKGFEEFETAAL
                     VEKTLAALQVPSERVAGTGVVGLLEGKRRDPAVGLRADMDALELEEKTGAPYASLVPG
                     LMHACGHDAHTAMLLGAAMLLSRRKSELEGSVVFLFQPAEETTGGARPMIEEGVLENP
                     RVEAVFGLHVGIGIPCGRIAVSAGTVHAASDMFDITVSGKGCHGAYPHEGIDALAAAG
                     QVVCALQAVVSRGIDPLEPAVVSIGTIRAAPRETWSRNRSGWRASCGPSRRKPAGSRG
                     KGSKRLQAGPPRP"
     gene            complement(7575..8546)
                     /locus_tag="PLY83_02275"
     CDS             complement(7575..8546)
                     /locus_tag="PLY83_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOV28893.1"
                     /translation="MTEPLVSVRNLKKYFPVKAGMFGSEDRVVKAVDDVTFDIARQET
                     FALVGESGCGKSTLGRTILRLVEPTSGSVMLGGGELTSLKPEALRRMRTRMQIVFQDP
                     YASLNPRMRVGDIIAEPLLTHEKGTHAEREARVLELMRVVGLRADCIRRYPHEFSGGQ
                     RQRIGIARALALNPEFIVCDEPLSALDVSIQAQVLNLLADLQAEFKLTYLFITHDLSV
                     VRHFAPRVGVMFLGKMVEMGPTEDLFTSPRHPYTRFLVSAVPIPDPRQRDRKKLLIEG
                     EIPSPMNLPSGCRFRTRCPFAAPVCAEIDPPSVFEGERSWACHFPLK"
     gene            complement(8543..9544)
                     /locus_tag="PLY83_02280"
     CDS             complement(8543..9544)
                     /locus_tag="PLY83_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV28894.1"
                     /translation="MKTSETLLSIENLSTSFFTSKGVVPAVSGVSLSVERGEIHGIVG
                     ESGCGKSVTSLSIMGLVAPPGRVVEGRILFQGRDLTKLDNNAMCELRGNEIAMIFQEP
                     MTSLNPVFTVGRQVSESILIHRKVPSEEAKRQAIEMFRLVGVPEPEARYDCYPHQLSG
                     GLRQRVMIAMALVCEPKLLIADEPTTALDVTIQAQILDLMQSLREKLRTAIILITHDL
                     GVVAELCDRVTVMYAGTVVEEAGICELFDSPSHPYTQGLMGSIPGTAASRGLDGRLRT
                     IPGMVPNLLNLPSGCRFHPRCSMARAVCKNQVPPLFDLGGGHTARCWNYEGKAVVQP"
     gene            complement(9564..10487)
                     /locus_tag="PLY83_02285"
     CDS             complement(9564..10487)
                     /locus_tag="PLY83_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOV28895.1"
                     /translation="MNDPKEQDLTLACCAAGTCEEEESIAPPSSFLKDVVIAYRRNRL
                     AMVGAAIVLILFLMAVFAPWLTPYDPYAIDLEVMTEAPSWSHWLGTDQFGRDLLTRIL
                     YGTRISLVIGIVPTLLSMIIGTTLGLIGGYYGGKTDMVIMRLCDIVLAFPSLLLAMVV
                     MYTLGATLVNIFIALAAVGWAGTARVVRSQVLSLKEKEFVEAARAVGVKNRVIMVRHI
                     LPNCLPALIVLFTLGIPGAILSEASLSFLGVGAQPPTPSWGLIVTSGKEFLFSAPWVA
                     IAPGLFILVTVLAFNFMGDGFRDALDPYMKQ"
     gene            complement(10501..11421)
                     /locus_tag="PLY83_02290"
     CDS             complement(10501..11421)
                     /locus_tag="PLY83_02290"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOV28896.1"
                     /translation="MLKFILKRLLISIPILIGVTVIIFFLLNVVPGDPVALMMKEHIS
                     VDVVARVRAQMHLDDPAIVRYFRFLAGALRGDLGESYKLNRPVTSLILNAFPNTFVLA
                     LWAALFAWVVGIPAGILSAVKKYSLPDNLFMGFSLLGVSMPVFWSALLFQYVFAVKLR
                     WLPVAGFEDWRYVIMPAIVLGWSSAGIIARLTRSSLLEVMRNDYIRTARAKGLHELPV
                     IFGHALKNSLLPVVTVMAIQVAGLLAGAVITESVFGIPGVGRIAVNAIQARDMPLLQG
                     AVLFETTLVILGNLAADILYAFLDPRIKAA"
     gene            complement(11501..13096)
                     /locus_tag="PLY83_02295"
     CDS             complement(11501..13096)
                     /locus_tag="PLY83_02295"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOV28897.1"
                     /translation="MRKSRLFVVLSLSFLLAGILAAGAFAAEKVFTIAYNEEPQTSDM
                     QMTTSCYMIPMNVYDRLIECVTTAPGKSALVPGLATKWEVSKDGKVYTFYLRKGVKFH
                     NGEELTAQDVVYTFDRMLDPKTKALNTDILDFVLGAKDRMEGKAASTAGLKALDRYTV
                     RITLEKPFVPFLAIMASPQASILNEKFTRPLGNKYGLTAQATCGTGPFILKEWVLNDH
                     HTMVANPNYFRGRPQIDKFVVKIVTDPETMRMLFESGEIDLFDCDFAMSQIPYFTSHP
                     KWGKQTVSGPRVGIYYYTFNQAIKPFDNVKVRKAFQMAVDRKLILQKMFYNHGQLENG
                     VMPRGLIHYNPNQEKIEYNPEKAKKLLAEAGYPNGVDMEIALVASTGSQWQKMSEIVQ
                     AMVAKAGFRVTLKQMDEAAFYATRKEGKLPMYTNKWSADFNDPDNFFYTFFGPKGTVA
                     RSYNNISPEVFAALEKGRSETDFAKRGKLYADLEKKIVIDQAAWLPMFSLKHIYVVQP
                     RVKKFVVPWNGWSDFPIYETRVE"
     gene            complement(13219..14253)
                     /locus_tag="PLY83_02300"
     CDS             complement(13219..14253)
                     /locus_tag="PLY83_02300"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOV28898.1"
                     /translation="MTETLQAAERLTRESLVIDVHYDLGMDLCDRYDRGESRIWPEYE
                     SFFSAGGVKGLVSSLFLHSYFLPEMALRKALDQIACLLKEQEAFKDRVSICRSASELE
                     EAAAEGRTGILLSFEGVEPLGNDLSLLRVFYELGVRGVGLAWSRRNEAADGCHFAPKR
                     EGRKGGLTPFGVQVVEEAERLGMWIDVSHLNDEGFWDVAAIARGPLVASHSNCRALVP
                     GMMRNLTDDQIVAIAKTGGVIGMNSCSAFVKPDHDRRRLGPEDLADHVDHIAKLVGSQ
                     HVCLGFDFCDGFKDYLTMGCSVPTYDVVAGHREIPAFAAVLLQRGYSEDEVRGILGGN
                     AMRVFKKVLA"
     gene            14368..15597
                     /locus_tag="PLY83_02305"
     CDS             14368..15597
                     /locus_tag="PLY83_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOV28899.1"
                     /translation="MNKPSIGIERLRAFFGLNQSLSGLLFMVILVGLGEKMAERFLPL
                     YLLALGAGTQSVGFLNAMDNLLGALYSFPGGALSDRWGAKRALMAFTALAMAGYSLVI
                     LVPAWWAVLAGAVLFISWTAISLPGIMSLVSTAVPKNKRVMGVTLHSFVRRIPMALGP
                     ILGGALIARYGTVSGVRIAFTAALVLGGLSLFIQWKFIADTREHGKSPLHPAESWKRF
                     SPALRSLLGSDILIRFAEQIPYAFVVVWIVRVRGLSAAQFGLLTAIEMVTAMLVYIPV
                     AAMADRYGKKPFVLMTFVFFTAFPLSLLFCHSFSAFALAFVLRGLKEFGEPTRKALIM
                     DLAPEDAKASTFGTYYLIRDVIVSAAALSSATLWNISPAVNFLTAFGFGLAGTLLFAA
                     FGKDIPQGAGTSPLSPK"
     gene            15691..16317
                     /locus_tag="PLY83_02310"
     CDS             15691..16317
                     /locus_tag="PLY83_02310"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOV28900.1"
                     /translation="MSAKAVPTRGNLVRLARSLELARKGHDLLEQKRQVLMMELTATI
                     AKARDLQKEVAAVFEEAYAALQRANVSLGIESVEEIAHAIPEEKNFTIRLHSVMGVEI
                     PEVDPIEPKRGPAFSFWDTSGSMDVAALAFRKVLAALARLAEVENAVYRLAVQIRRTH
                     RRVNALEKVVIPTTLSTLASIASVLEEGEREDFVRMKTAKKSRGERGG"
     gene            16321..16668
                     /locus_tag="PLY83_02315"
     CDS             16321..16668
                     /locus_tag="PLY83_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV28901.1"
                     /translation="MSSLQEALVVLLGAESEGVREVQEAKIQAEALLKETRERMAREA
                     ETRIQAARKKASAVLENARAAADEEARSVTEQGKSERERMERRFREIAGGVVDSLVQE
                     AVETLLGKRGGPS"
     gene            16665..17759
                     /locus_tag="PLY83_02320"
     CDS             16665..17759
                     /locus_tag="PLY83_02320"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HOV28902.1"
                     /translation="MTFFFSLGERTAAGAKAHVLFSKLLKDEEMWALLQCDDVPEIFS
                     QLVRSEGYGDALSSLVPQEVTRVQLEDRLKLLLPKRAAQFLPYLTGARRRFLLAWMER
                     DQAGALKTALRFVLTRHGDREAIRARIAALPRTSLPFDLLLSSRDFQDFSDALRGTPY
                     FHPLQEPLKRLALGEQNLFSAEAAIDREVLVRLLEAAKALSAGEMRSVMRLLGVRFDL
                     LNISWTYRSLRYYDLTPEERVARLLPFRYRLHGDSLRKMARAGEPDEFWTILKTTAYR
                     EAFGESPPIEEMVMDRDVRRYIRQTAYKVFRQGRPDLSTVFAYLTLLESEIRDLATLI
                     EDVRYDYDRRHAALFLARPLIPGGEVRWQW"
     gene            17747..19705
                     /locus_tag="PLY83_02325"
     CDS             17747..19705
                     /locus_tag="PLY83_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOV28903.1"
                     /translation="MAVVKMLAVTLIGPKAEMETVARLMVLTGGFQPLPLDLLLKDRS
                     VRSKITTASENPYDELLVKLSEVWKAAGERVPDPFPVPITPDFSLDRAAKAVNETVHR
                     LSLWSKRREELSEEREELEAAQILTASLSERGMNLADLADTTYLVPLFGKLTDENYRR
                     LEEATLEAPLLLEPLLSRHGFTWFLGLTAPGYREGAKKLLESLYFKGFSLKEMASSLA
                     GDPQVQISRRIENTRKAIEGLKRAAAEVLQSNRQGMERLYASIYTMQRVYDLCRGRGE
                     LSGLYLLSGWIPEDTLQRTLEILEREAPKTTVHIEELGEMPYTGIRVPTLLRNLPLVR
                     AFQDVVSLYSLPAYGEMDPSFFVAVSFCLFFGFMFGDVGHGLALIAAATILQRKRLLS
                     RSMGTVLKSAGAASMLFGFLYGSVFGVEGWIPALWISPMEDMNRLLGVSIGVGVSVIT
                     LGMILNMISCYKARDFGRLLFDGKGLAGMVLYLSMAAIGYAVLTGQRMPFPSWVPWTF
                     LSALLTVILMRDFLARLLLRQRTHEEGGLYAFEVLHNLLNFLSNTASFVRLAAFALNH
                     VGLSLAVMMLSDMVRELPGGILLKGVLLLAGNAVIIALEGLIVFIQTLRLEYYEFFSK
                     FYRGGGRAFRPVSFRDPRLQAGSPTPGS"
     gene            19729..20145
                     /locus_tag="PLY83_02330"
     CDS             19729..20145
                     /locus_tag="PLY83_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HOV28904.1"
                     /translation="MFGLLVLIIASLTFGLLGARLSGRAVKNPRGVLAASLFACSVSL
                     LAGSLLALCTGPAAAAEAGATLGAGAGMGFIGAAVSTGLACLGAGIAVAVVGAAALGI
                     IGEKPEMLGTTLIYLGLAEGIAIYGVIVALLILGKI"
     gene            20152..20463
                     /locus_tag="PLY83_02335"
     CDS             20152..20463
                     /locus_tag="PLY83_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HOV28905.1"
                     /translation="MRAYLLSDNLDSLVGLRLAGIEGRIARGAEECRKAIEEVLEDPE
                     IGILVLTEKVAETVPDLVQGLRERGELPLVVEIPDRHGMRRAADFLTAYVRDAIGVNL
                     E"
     gene            20460..21074
                     /locus_tag="PLY83_02340"
     CDS             20460..21074
                     /locus_tag="PLY83_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOV28906.1"
                     /translation="MNEIQNEKLNGLRELLLLQAEQEKMGILEKAKKEAESWIAQEEE
                     RISREEELILKDAADRAEATRRRQVMAAEREKAAEGLRLQNRLLSEAVSMLEDRFCRL
                     RERNDHVRILAGLIAEAESALPESGGLRFRLAGTDAPIGEAVAKEAEALLPGIEVRFD
                     PDPAPILGGAWIWTADGRRFVAADWRNRAQEMAGTLAERLLPML"
     gene            21102..22883
                     /locus_tag="PLY83_02345"
     CDS             21102..22883
                     /locus_tag="PLY83_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOV28907.1"
                     /translation="METFGTIVRVNGPVVRARGMRSFGMREMVRVGKLGLWGEIIRME
                     GDEALIQVYEDTQGLRLGEPVEGTGEPLSVSLGPGLIGQIFDGIGRPLGALFEREGPL
                     LSRGIQVSMIDPQRSWEFSPSARKGDVVTAGMVLGTIQETPLFEHRILVPPDLEGEIT
                     DIRTPGTIRGEDVVARIRDARGRTVQVPAIQRWPVRAARPYRERLLPTEPLVTGQRVI
                     DGLFPIAKGGTAAIPGGFGTGKTVTQHQLAKWSEAQVVVYIGCGERGNEMTQVLEEFP
                     VLEDPRSGRPLMERTILIANTSNMPVAAREASIYTGITIAEYYRDMGYDVALMADSTS
                     RWAEALREISGRLEEIPAEEGFPAYLPTRLAEFYERAGRVVTLGGNEGSITIIGAVSP
                     PGGDFTEPVTRNTKRFIRCFWALDRNLANARHFPAIGWLESYSEYAQEVENWFGTHVD
                     SRWGQLRERARALLAEDEKIQQVIRLVGEDVLPDEQRLIAFTAFLIKNGYLQQGAFGE
                     DSFSPPEKGFAILELMLHFHDKAGELVKRGVPLSLIRDGRPVLALTHLRERPLNDPKG
                     GPAALRKELDAFLEKVGTERSAAKGRM"
     gene            22886..24259
                     /locus_tag="PLY83_02350"
     CDS             22886..24259
                     /locus_tag="PLY83_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOV28908.1"
                     /translation="MAIAEYIGLETINGPFVLVEGVQGAGYGELTDVIGPDGKVRKGR
                     VVVLDERATLVQVFSGTGDLVPESTRVRFLGKELSMTLDPAILGRTFTGLGEPRDGCG
                     PVYGRVTRNVNGQPINPMARAYPRDFIHTGISSIDVLTTLIRGQKLPIFSGNGLPHNA
                     LAVQIATQSRLVGAENFAVVFAGIGIKHDDAAFFSRELSDKGRTDKIVTFLNLADDPV
                     IERIATPRFALTAAEYLAFDLGLHVLVILTDMTNYCEALRELGVAVGEVPSRKGYPGY
                     LYSDLASLYERAGVLREGKGSVTQLPILTMPNDDITHPIPDLTGFITEGQIVLSRELD
                     AKGIYPPIDVLASLSRLMKDGIGKGFTREDHPNLSSQLFASVSRVQEVRALASVVGSD
                     ELGKQDKASLAFGELFENQFLSQGTGEDRDIGFSLDKGWEILSVLPREQLTRISTEEA
                     RKYLAQK"
     gene            complement(24301..24498)
                     /locus_tag="PLY83_02355"
     CDS             complement(24301..24498)
                     /locus_tag="PLY83_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOV28909.1"
                     /translation="MMSPLHTKYVCTVCGYVYDPQVGDPKAGILPPMPFSDLPEDWIC
                     PECGVGKDLFTLLTPGPRVAD"
     gene            complement(24543..26384)
                     /locus_tag="PLY83_02360"
     CDS             complement(24543..26384)
                     /locus_tag="PLY83_02360"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HOV28910.1"
                     /translation="MRCISAKVFDIDLTKGSIQEKQLPEEYWQLHLGGSGVAARLLYD
                     EGTYAVDPLGPENILVFMNGLFTGTPVPCGCKMAVCAKSPLTGIWGEATVGGYFGAEL
                     RKTGYEGFLVRGKAQRPSYLLIENGQVSIREAEDLWGLDTYKTSELLKDRHGEKYEVA
                     AIGPSGEKLVLLAGIMFGGSETRAAGRTGLGAVMGSKNLKAVVIRGTNPVDLFDRPGL
                     VQSIQETVPVIKANTKGLTDFGTAGGVMAVEANGDLSIRNWSLGSWTEGAAKTNGQKI
                     AETVFVSHYGCFACPIRCGKNVRVPVGPFKGQLAHGPEYETTDGFGANLLNEDLDVIV
                     AANDLCNRYGLDTISTSGVIGWAMECYENGLITKEETGGIEITWGNGEAILKLTELIG
                     KREGFGEFLGQGVKRAAEKLGGLAVEFAVHTKGLEYAFHDPRAFTSMAANYATANRGA
                     CHLEGLTYFAEQGAFPLSLVDFEKEFTRHGHENKAEIAVIMQNFMEAMNNLGLCKFLI
                     RGKDSTSPAIFAQWIHQVTGIPYSKAELMKAGERNFNLKRLFNGKIGITRKDDALPPR
                     LGVHDKESGAAKGSIPYMGKILWDYYRIRGWTPEGIPGKEKLRELGL"
     gene            complement(26456..27619)
                     /locus_tag="PLY83_02365"
     CDS             complement(26456..27619)
                     /locus_tag="PLY83_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOV28911.1"
                     /translation="MMRPTEFRMPQVLLQGPGCLANIGKEVKRLGKKKALLVTDKIMV
                     KAGYAAQVEKILADVQVESVLFDGVGTEPTDVYVEEGLKVYRESECDFLVGLGGGSPI
                     DTAKAVGILATNGGQITDYMGADKVAVPTPPVVAIATTSGTGSEVTKFTIIADTKNNV
                     KMLIGSPFVIPMIAVADPVLTLSVPPQVTAATGLDAFCHAVEALVSKKEQPMTDLLAL
                     NAIKLIGTYLRKAWCNGDDLEARAQMMLASTQAGLAFNNASVTLIHGMSRPIGAVFHV
                     PHGISNAVLLPVWAEFTYIAKPEKFALIAEAMGEKVAGLSSLEAAEKGVEAIKRLCRD
                     VEVPPIRTLVPDREAFEKPLEKMAHDAVVSGSPGNNPRFVTEAQIVELYKKAY"
     gene            complement(27697..29001)
                     /locus_tag="PLY83_02370"
     CDS             complement(27697..29001)
                     /locus_tag="PLY83_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV28912.1"
                     /translation="MDFLQVAILPIALILFGVLAFQKWSALILGPLLALLVAAVAGLP
                     LMDTMLGPYMETAAAYIKSFFLVFFVGSVFGSVMEETGAAKSLAQWMTSLTKGKYVAP
                     LIMTVTGILTYGGISGFVIFFAMYPVALHMFKEANLSRRLIPATISAGCWTWSMSGPG
                     TPAIQNVIPMRFLGTSPLADPIGGTMTTLLQFVLIFIYLEWQGRQYARKGFTFVEDEH
                     VRKFTSSLDVAQKLPNPVISIIPCILILVLFNVFHVKVEAAVLFGILAGFLLLMKYGG
                     GYQQWVSIFNNGAMNSAPAILNTAIVVGFAGVVRQTPGFTTLIDGLKGLNMPPLLFVA
                     TVVAVAAGAAGSASGGLSVAFAALKDTFVGMGVPLPWVHRISVIAAGTLDTLPHQGAQ
                     ITLLAICHQTHREAYWPIAVTQIFVPMIAVAALILYHAMGLP"
     gene            complement(29078..29311)
                     /locus_tag="PLY83_02375"
     CDS             complement(29078..29311)
                     /locus_tag="PLY83_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV28913.1"
                     /translation="MRINIHLKGHLSYYQSEKREEVVLDVEAPVTLGEILSQLKIPVS
                     EVEGAFLDGKWVALSESLERGGKLDIMPIIGGG"
     gene            29535..31088
                     /locus_tag="PLY83_02380"
     CDS             29535..31088
                     /locus_tag="PLY83_02380"
                     /inference="COORDINATES: protein motif:HMM:NF019520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HOV28914.1"
                     /translation="MIVADLLHQHIFPDFRLVAGNEGLTREITAISVIDAPDMASWLK
                     GGEFLFGTGYIFKDSQSALSLFFHEVAGRGIAAMGLKLDRYHAKFPETLLQEANELKI
                     PVFEIPFHYRWSDIIHEIQSRLYHQDQERLGQSRYAPPLLWSSDWDSKRLISTLATEI
                     QKEILVEIHTLQMKYGFAPDGSPIESDRFNEVKTAFAVREIELPRKGNIYISLEEKEV
                     QEGKLWTANYSLKTETPITLTLILNKEENHPSLRDERLVLRAISLLRSEALELALASN
                     QLSAKRERFLENLCLGSYQNQDMAAQRAKNLGVSIPFPCQVFGVATMERITLPLWMPP
                     YENRYALGNFWVVIESSKKLEQDAETLHEIARDNHLWIAMGSVADDFPGIQRSYEETK
                     KALYWIRDFKPRPGLYRFSDLALHALLGRLSRMPESEGLWKRFWMPLKTIDSSRRAIP
                     LEEVLNSLILCDFNAKKSADLLHIHYNTARNYLREIEELLRVDLNNRLHRLALSLAHQ
                     IEIAKSKES"
     gene            complement(31097..32011)
                     /locus_tag="PLY83_02385"
     CDS             complement(31097..32011)
                     /locus_tag="PLY83_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOV28915.1"
                     /translation="MKSFVVAGCQFAVTPMDVEANIRKAIVFLERAVHEHGADLVVFP
                     EAITTGFTPPPRPTQEETVTALWEAVDCIPGKTTLPIQEAAARYGVSVIWPTYSRGNE
                     NDIVYNSVVAIGSDGKILGDVYHKTHPFPTERKEGGGWTTAGNKAVVVETPFAKIGLS
                     LCYDGDFPEFSIVETLMGAEVIVRPSSLLRSYNVWAFTNFARAYDSHVYYVAVNAVGQ
                     DASGNNFFGSSMIIDPTGWLLAQASGNEEIVSARLDPDPMRFHAHGTRTPMLFDHMED
                     RNLEVFREWILKEGKAPFEPSRRIPYKR"
BASE COUNT         6281 a        10024 c         9664 g         6296 t
ORIGIN      
        1 ctcctccccg gttcggacca tcgtttgcct gacctttgca ttgacgttat cataggacga
       61 attccaggcg ggaaccacgc attcaaggac ggtccttcat gttgccgaga tccggtggtg
      121 aacctcttcc cggaacgggt ccctttcggg ccctccgcta tcgaaactac aggctttttt
      181 tcgccgggca ggccatctcc ctgatcggga cctggatgca gcgggtggcc atgagctggc
      241 tcgtctaccg gctgaccctc tcgcctttcg ccctggggct cgtcgacttc gccagccagg
      301 tcgccactct tttgctgggc ttcatcgcgg gagccgttgt ggatcgtacc gacctgcgcc
      361 gccttctcgt ctggacacag gggctggcca tgggacaggc cctggtcctt tccgccctga
      421 ccctttcggg aaaggccacc tttaccgtcg tcctcgtctg cggagcgctt ctcgggatga
      481 tcaatgcctt cgacatgccg gcccggcatt ccttcgtgat ccggatggtg gaaaggaaag
      541 aagacctggg caacgccatc gccctgaact cttccctgtt caacctggca cgcctgctgg
      601 gtccggccgt cgccggattc gtcatcgccg ccgtggggga aggcctctgt ttcctgctga
      661 acggcctgag ctactccagc atcatcatcg ccttgaacgc catgcacctg gcccccgcct
      721 cgagcgaacg gccgcaggaa gcggccctgg ccggcatccg agagggcttt tcctacgcct
      781 ggatccgaag gcccatccgg gataccctga tcctcctctc ctgcctgagt cttttagggc
      841 tttcgtatgt tgtcatgatg ccggtcttcg cgaaagagat cctcaagggg gggcccagga
      901 cactcggctt cctgatgggc gccaccggtg ccggagcact ggccggatcc atgctcctcg
      961 ccgtccgcaa gggcacgaag gggctggaga aaggcatccc gatcgcgtcc ggtctctttt
     1021 ccgcggccgt gatcgggttc gctttctctc cctccgcagg ggcctcctac gtcctcgtgg
     1081 ccctggccgg cttcggcatg atctccaccc tggccagctg caacaccctg ctccagacgg
     1141 tcgtggacaa cgcgctgcgg ggacgggtca tgagcctcta caccatggcc ctggtgggca
     1201 ttgccccttt cggaagcctt ctcgcagggt ggctgacgga ccggatcggg gtccggctgg
     1261 ccctggcgct cgggggagtc tgctgcatgg cagcctcggt gtggttctcc cggcgcgttc
     1321 cccttttcaa caaaccgctg ttcccggaag gcgaaagacc tttttgaaac cgaggtgatc
     1381 ctcctgaaga tcgcagtctt cctcaagcaa gtacccgaca cggacgaagt ccggatggac
     1441 gagaagaccg ggacgctgat ccgggagggg gttgggaccg tcatcaaccc cctggaccag
     1501 aatgccctgg agctggccct ttctctccgt gcggccaagg gcggcagcgt gacggtcctg
     1561 tccatgggcc cggcccaggc ggaactggcc ctgcgcgaag ctctcgcact gggagccgac
     1621 gaggccgtgc tgctctgcga ccgggccttt gcgggaagcg atacctgggc aaccgcccgg
     1681 actctcgcgg ctgcggtgcg taaaaccggc ccgtatgacc tgttgctggc cggggcaaag
     1741 gccaccgacg gggaaacggg gcaggtcggc cccgaaagcg cctccatgat ggggctcccc
     1801 ttcgtgtccc acgtctgcgc ggtcgaaatc caggcagaat gcctggttgc gtctcgatcc
     1861 gtggaggacg gaacggaacg catccgggct ccgcttccct gccttttgac cgtggtccac
     1921 gagatcaacg aaccctccat gcccaccctg gccggaaaga tgcgggcccg gcgagccccc
     1981 gtccgtgtcc tgtcagcggc ggacctggga ctgaatccgg cagagtgcgg gcttgcgggt
     2041 tccccgacga gagtcgttcg catcgccttt ccgaaagtca cccgcagcgg cctgcgcttc
     2101 agtggaagcg agctggaccg gggcatcgcc cttgtggctg aaaggctgga cagcatggga
     2161 ctgctgggcc agaaacgtcc ggcacgatcc gtcgattcgg ctcgttcccc ggaggcggcc
     2221 cgaccgattc ctccggttga gcttgcctgg gaggaagcct tcaaaaacga tcctgtcggg
     2281 ggcgttctcg tctgcggcga gtgccgaaac gggagcatcc acccttcaac cctggaactg
     2341 gccggtaagg cccgggaact ggccgacagc cgtggcagct ccgttacggt ggttcttcct
     2401 ttcggccatc tcgacgacga tccggcccgc tgcatccgcc atggggcaga ccgcgtcctg
     2461 gcggtctgcc atccggccct gggcctttat aacccggacg tcgaaagccg gcttttcgct
     2521 accctgattc gaaggctccg cccggaaatc gtcctggctc ccgccacgac ctccgggaga
     2581 accaccctgc cggctgtcgc cgccctggtg gatacaggac tgacggcgga ctgcaccggg
     2641 ctttcgatcg accccgaaaa cggggacttg ctccagacac gtcccgccat cgggggaaac
     2701 gtcctggcga ccattcggac ccccagccgc aggccccaga tggccaccgt gcgcccgagg
     2761 accttccgcc tcccgccgga agacccctcg cgccgcgggg aggtctaccg ccttacgccg
     2821 cctgaacatt tcctggaatc ctccctggtt cccctcggcc gagaaacccg ccaggaggaa
     2881 ggcgccccga tccaggatgc cgaagtcgtg gtttccgggg ggaagggact gaagcggccc
     2941 gacggcttcc gcctcgtgga ggagctagcc gccgtcctgg atggagcggt aggagccagc
     3001 cggcccactg tcgaggccaa atggatcggg tatccccacc aggtgggatt gagcgggaag
     3061 gtcgtgacgc ccagggtcta catcgccgtc gggatttccg gggcgatcca gcacctggcg
     3121 ggcatgcaga cttccgagtt catcgttgca attaaccgcg atccggaagc cggcatcttc
     3181 cgggttgccg acatcgggct ggtcggcgac cttttcgaca tcgtcccgcg catggcaggg
     3241 gcgatcaaag ccagaaagga ggccccaaaa tgaaggaacc ctcccgcttc ggaacggtca
     3301 ccccggaagt cgtcgaacgg ctgtctgcca tcctgggaac cggcggggtg tccgtagacg
     3361 gggaaaaaat cgccgcctac tccagggacg aagtggcgga acgtttctgg gaccgtccct
     3421 accgggccga ggtccttgtc ttcccggagt cgaccgggca cgtctccgag atcctgcggc
     3481 tggccaatga acgccggatc cccgtcaccc ccaggggggc aggaacagga ctctcgggag
     3541 gagccgtccc ggccttcggg gggatcctgg tgtccttcga gcgcatgaac cgtatcctgg
     3601 aactggacac ggagaacctc tgcatgaccg tggaacccgg tgtgatcacg gcggaaatca
     3661 cccgtgccgc ccggaaagcg ggcctgcttt acgcgggaga tccctgcagc ggggatgcct
     3721 ccttcatcgg cggaaacgtg gccgagaacg ccgggggcaa caaggtgatc aagtacggcc
     3781 ccacgggcag ttccgttctg gccctggagg tcgtcctggc ggacggctcg gtgacctggt
     3841 tcgggggcaa gcggcgcaag gacgtgacgg gatatgactt cgtccacctg atggtgggtt
     3901 ccgaaggaac cctgggaatc atcacgaaga tcgtgctgaa actgcttcct ctcccggcct
     3961 tcggcgtgga cctcttgatc cctttcccca ccgtggaagc cgccatccgc ttcgttcccc
     4021 ggatcgccct cgaggggcgc gtcacgccgg cctccatcga actggtggac caccactcgc
     4081 tctcccaggc ggaaaagttc ctcaacacgc ggctcccctt cgaccaggcc gccgcccacc
     4141 tgatcgtcca gctggaaggg aacgatcggg aagtggtggc agacgagaca gagagagtgg
     4201 gaaagctggc cctggaaaaa ggggcgctcg aggtcttcgt ggcggacaac cggtcaagcc
     4261 gggacaagct ctggaaagcc cgaaaatcgg tggcagaagc catcatggcc tattaccaga
     4321 actatgccaa ggaagacctg gtggttccca ccgaccgggt accggacctg cttttcgccg
     4381 tggacgccct ttgcaggaaa tggaggatgg aagccgccaa ctacgggcac gtgggtgatg
     4441 ggaacatcca cgtgaacctt ctgatggccg acgatcgccc ccactggcac gaagacatgg
     4501 aacaggcccg gcaggagctt tacgaaacgg tccggcagct gggcggaacc ctttccggcg
     4561 agcacgggat cgggctgaag cggaaggctt acctgccgca gttcctcgac gcgccccagc
     4621 tggaactcat gcggagagtg aagctcgcct tcgacccgaa cggcatcctg aaccccggca
     4681 agctcgtctt ctgggacaag aaaacggagg aagcgggggc atgaggccga gaatcgggat
     4741 cacctgcggc ttcgcagggg aaagggaccc gctggcgctc gaccgggatt atgccgatgc
     4801 cgtcgaccgg gcgggcggag tccctctcgt cgtgccgccg ggagttccag gagaggccct
     4861 tgccgaaacc ctggaagccc tggacggcct cctgttttcc ggcggcccgg acgtggatcc
     4921 tgcccgtttc ggagaggaac cccttccggg catgggccgc atcgaccccg ctcgggacga
     4981 ggcggaactt ttcctcgccc gcctggggct tgaaagggag atgcccctcc tggggatttg
     5041 ccgcggggca caggtcctga acatcgcatc cggaggggat ctttaccagg atctcccctc
     5101 ccaggtggag ggcgtcctga aacacgtgca gcagcccact cccatgtcct acccgaccca
     5161 caggatccgc atcgagccgg gaagcctcct ggcccggatc acggggaagg tctccctcag
     5221 ggtcaacagc tggcaccacc aggcgattcg gcgcgtcgct ttcggtttta tcccaaccgc
     5281 aagggcggaa gacggagtga tcgaagccat cgaaaaaacg ggggaaccgt tcgtcctggg
     5341 cgtccagttc catcccgaaa gcatgggaag aggaggatcg cccgaggcga tcctcctctt
     5401 cgaagcgttc gtccaggctt gcgcccgctg ggccagggac taggaaaggg cggcttccag
     5461 ggccgtcagg cggcaggcgt ttgcatccac ctccacctgg attcccagcg ggagggtgag
     5521 attcagtttg ccgtggccgc aacgaagtcc cctgacgacc gggatcccga ggccagggag
     5581 gtggtcctcg aacacctcgt ccagggtcag gctgtcctcc gggcccggag aaacgggatc
     5641 ggcattgagg aagtccccga tgaccacggc agcggccgcc gagagcttgc ccgcgttgcg
     5701 caggtgagtg agcatgcggt cgacgctgta gggttgttcg ttgatgtcct cgatgaaaag
     5761 gatcttcccc ctcgtatcca gttcataggg agtccccatg gtggagcaga tcagcgccag
     5821 gttccccccg acgatctccc ccctcgcttt tcctcctgcc agggaagtga gccccgatga
     5881 agcgtcggcg aggactccaa ggggttccgt cgaagtcagg gctttcagga aactttccct
     5941 cgtgatccct ttctctccct tcgaggagaa ctcgatcgtc gccatggggc cgtgaaaggt
     6001 cacgaaattt ccctgctgcc cgaagaccgt atggaagacc gtgatgtcgc tgtaccccac
     6061 gaagggcttc gggttcgccg cgatgagccc catgtccagg cccggcagca gccgacccga
     6121 accgttccct ccccgcaggc agacgattcc gtggatcgat ggatccgaga aagcccaatg
     6181 aagatccgca agcctcgcct cgtccggtcc ggccatgaag cctcttttca ggaaacaggt
     6241 cttcccgaag acgggctcga aaccgagctt cgcgagcgag tccgcccccc ttttcagttt
     6301 ttcctccggg gcggcgcttc ccggcgctac gatggcgatc cggtccccag gcctcagggc
     6361 cttcggcttg attctcatgg tcctacctcc cctgcaggaa attcagtgcg actcccgcca
     6421 gcatggcggc tccgattgga agcgaatcct catccaggtc aaaccgcgag gaatgcgccg
     6481 gatggacgat cccctttgcc ggattccccg cacccagagt gaaaaatgcc gccttgcgct
     6541 cccgggcaaa aaaggaaaaa ttttccacac ccatgctggg tctaggcact tcgagaacgt
     6601 tttcctcccc gaggagaaga cagccggttt cgcggacgag agccgccatg gacgggtcgt
     6661 tttccaggca gggatagccc tcctggaagc ggacctccgc tcgggcccct agggcctcgg
     6721 cggcccggct tgcaaccgtt tcgatccttt cccgcgcgag cctgcgggct tccggcgaga
     6781 gggtccgcag gatgccctcc atcctgaccg attccgcgac cacgtttctc gcggagccgc
     6841 ccggatcgtg ccgatggaga cgaccgccgg ctcgagaggg tcgatcccgc gggagacgac
     6901 ggcctgcagc gcgcaaacca cctggccggc cgccgccagg gcgtcgatcc cctcgtgagg
     6961 ataggcgccg tgacagccct tgccggacac ggtgatgtcg aacatgtccg atgccgcatg
     7021 aacggtaccc gccgaaacgg cgatcctgcc gcaggggatg ccgatcccca cgtggagccc
     7081 gaaaacggcc tcgacacgcg gattttcgag aactccctct tcgatcatgg ggcgcgcccc
     7141 gccggttgtt tcttcagcgg gctggaagag aaagacgacg cttccttcca gttcggactt
     7201 ccttcgcgac aggagcatgg ccgcacccag gagcatcgcc gtgtgggcat cgtgaccgca
     7261 ggcatgcatc aatcccggca cgagcgaagc atacggggca cctgtttttt cctcgagctc
     7321 cagcgcatcc atgtcggccc gcaatcccac cgcggggtcc cggcgcttcc cctcaagaag
     7381 gccgacgaca ccggtcccgg cgacccgctc ggacggaacc tgcagggcag ccagggtttt
     7441 ttcgacgagt gccgccgttt cgaactcctc gaaccctttc tccggacgcc ggtggatcga
     7501 ccgccgaagc cgaacgaggt cttcgtgaag gccaatggcc tcctccagga ggccgtccag
     7561 gaatcgccga ccggtcattt cagcgggaaa tggcaggccc agcttcgttc cccctcgaac
     7621 acggaagggg ggtcgatctc ggcacaaacg ggggcggcaa atggacatcg agtgcggaac
     7681 cggcatcccg agggaagatt catggggctc gggatctccc cctcgatcag gagctttttc
     7741 cgatcccgct gcctcggatc cggaatcggt accgctgaaa ccaaaaaacg ggtgtaggga
     7801 tgcctgggcg atgtaaaaag atcttcggtc ggccccattt cgaccatctt ccccaggaac
     7861 atgaccccaa cccggggggc aaaatggcgg accacggaca ggtcatgggt gatgaacagg
     7921 taggtgagct tgaactcggc ctggaggtcc gccaggaggt tcagcacctg ggcctggatg
     7981 gacacgtcga gggccgacag cggctcgtcg cagacgatga attccgggtt gagcgccagg
     8041 gcccgggcga tcccgatcct ctggcgctgc ccgccggaga actcgtgggg atacctgcgg
     8101 atgcagtccg ccctgagtcc gaccaccctc atcagctcca gcacccgagc ctcccgttcc
     8161 gcgtgcgtcc ctttctcatg ggttagaagg ggttcagcga tgatatcccc gaccctcatg
     8221 cgcgggttca gcgaggcata gggatcctgg aagacgatct gcatccgggt cctcatcctc
     8281 ctgagcgcct ccggcttcag ggaagtcagc tccccccccc ccagcatgac cgaaccggac
     8341 gtgggctcca cgaggcgcag gatcgtccgt cccagggtgc tcttgccgca gccggattcc
     8401 cctaccaggg cgaacgtctc ctgccgtgcg atgtcgaagg tcacgtcatc caccgccttg
     8461 acgactcggt cctccgatcc gaacatcccg gctttcacgg ggaaatattt tttcaggttc
     8521 ctgaccgaaa caagaggctc ggtcatggct gtaccaccgc cttgccctcg taattccagc
     8581 aacgggccgt gtgtcccccg cccaggtcga aaaggggcgg aacctggttt ttgcagacag
     8641 cccttgccat ggagcatctt ggatgaaaac gacagcccga agggaggttc agcaagttcg
     8701 gaaccatccc gggaatcgtc ctgaggcgtc cgtccaggcc tctcgaggca gccgtcccgg
     8761 gaatggagcc catgagtccc tgggtgtagg gatggctggg cgaatcgaac agctcgcaga
     8821 ttcccgcctc ttccaccacg gttcccgcgt acatgaccgt cacgcggtcg cacagttcgg
     8881 ccacgacccc caggtcatgg gtgatcagga tgatcgcggt ccggagcttt tcccgcaggg
     8941 actgcatcag gtccaggatc tgggcctgga tcgtcacgtc cagggccgtc gtcggttcgt
     9001 cggcgatgag gagcttcggc tcgcagacga gggccatggc gatcatcact cgctgcctca
     9061 gccctccgga cagctggtgc gggtagcagt cgtaacgggc ttccggttcc gggaccccaa
     9121 ccagccggaa catctcgatc gcctgtcttt tcgcctcttc actaggaact ttccgatgga
     9181 tcaggatgga ctcgctgacc tgcctgccta ccgtgaaaac cgggttcagg gaagtcatgg
     9241 gttcctggaa gatcatggcg atctcgtttc cgcgcagttc gcacatcgcg ttgttgtcca
     9301 gcttcgtcag gtcgcggccc tggaaaagga tccgcccctc cacaaccctc ccgggcggcg
     9361 ccaccaggcc catgatggag agagaggtca cgctctttcc gcagcccgac tcccccacga
     9421 tgccgtggat ctctccccgt tcgacggaaa ggctcacgcc gctcacggcc gggaccaccc
     9481 ctttcgaggt gaagaacgag gtgcttaggt tttcgatcga taacagcgtt tctgacgttt
     9541 tcatacgcgg ttctcctcgt tctctattgt ttcatgtagg gatccagggc gtcccggaac
     9601 ccgtcgccca tgaagttgaa ggccaggacc gtcaccagga tgaaaaggcc gggagcaatg
     9661 gcgacccagg gagccgagaa gaggaattcc ttcccgctcg tgacgatgag tccccagctg
     9721 ggcgtgggcg gctgggcgcc gaccccgaga aaactgaggc tggcctcgga gaggatcgct
     9781 cccgggattc ccagggtgaa gaggacgatc agggcgggca ggcagtttgg aaggatgtgc
     9841 cggaccatga tgacccggtt tttcacgccg acggcacggg cggcctcgac aaactctttt
     9901 tccttcaggg aaaggacctg ggagcggacc acccgggccg ttccggccca gccgacagcc
     9961 gccagggcga tgaagatgtt caccagcgta gcccccagcg tgtacatgac gaccatggcc
    10021 aaaagcaggg acgggaaggc gaggacgatg tcgcacaacc tcatgatgac catgtccgtc
    10081 ttccctccgt aatacccgcc gatcaacccc agggtcgtcc cgatgatcat ggagagcagg
    10141 gtcgggacaa ttccgatgac cagggagatc cgcgtcccgt agaggatcct ggtcagaagg
    10201 tcacggccga actgatcggt tcccagccag tgagaccagg aaggagcctc cgtcatgacc
    10261 tccaggtcga ttgcataggg gtcgtagggc gtaagccagg gggcgaagac cgccatcagg
    10321 aaaaggatca gcacgatcgc agccccgacc atggcaagcc ggttgcgccg gtaggcgatc
    10381 acgacatcct tgagaaacga ggagggcggg gcgatggact cttcctcctc gcaggttcca
    10441 gccgcgcagc aggccagggt aaggtcctgt tccttcggat cgttcattcc cattcccccc
    10501 tcaggccgcc ttgatccgcg gatccaggaa agcgtaaagg atgtccgcag ccaggttccc
    10561 caggatgacc agggtggttt caaaaaggac ggccccctgg agaaggggca tgtcccttgc
    10621 ctggatggcg ttcaccgcaa tccggccaac cccggggatc ccgaagacgc tttcggtgat
    10681 gacagcccct gcaaggagcc ccgcgacctg gatcgccatg acggtaacaa ccggaagcag
    10741 cgagttcttc agggcgtgtc cgaagatgac cggcagttca tgaagcccct ttgcccttgc
    10801 ggtgcggatg tagtcgtttc gcatgacctc cagcaggctg gagcgggtca ggcgggcgat
    10861 gatccccgca ctgctccagc ccaggacgat ggcgggcatg atcacgtacc gccagtcctc
    10921 gaaaccggca accggcaacc agcggagctt cactgcaaag acgtactgga acagcagggc
    10981 ggaccagaaa accggcatgg acaccccgag cagggaaaag cccatgaaaa ggttgtcggg
    11041 aagggagtac tttttcacgg cggaaaggat ccctgccgga atgcccacga cccaggcaaa
    11101 gagggccgcc cagagggcca ggacgaaggt gttcgggaaa gcgttcagga tgagggacgt
    11161 gacgggccgg ttcagcttgt aggattctcc caggtctccc cggagggctc ccgccaagaa
    11221 gcggaagtag cggacgatcg cgggatcgtc caggtgcatc tgggcccgaa cccgagcaac
    11281 gacgtcaacg ctgatgtgct ctttcatcat cagggcaacg ggatcccctg ggacgacatt
    11341 cagaaggaaa aagatgatga ccgtgacccc gatcaggatc gggatggaaa tcagcagccg
    11401 cttcaggatg aacttcagca ttcttcgaca gcctccgtct tgcgtactcg agaacggcgt
    11461 accccgggag cgtttgttct cccggggtac gcctcagaaa ctactcgacg cgcgtctcgt
    11521 agatggggaa gtcggaccag ccgttccagg gcacgacgaa cttcttgacc ctgggctgga
    11581 ccacgtagat gtgcttcagg gagaacatcg gcagccaggc ggcctggtcg atgacgatct
    11641 tcttctcgag gtcggcatac agcttgccgc gcttggcgaa atcggtttcc gagcgtccct
    11701 tttcgagggc cgcaaagacc tcggggctga tgttgttgta agaacgggca acggtcccct
    11761 tgggcccgaa gaacgtgtag aagaagttgt caggatcgtt gaagtccgcc gaccacttgt
    11821 tcgtatacat gggaagcttg ccttcctttc gggtggcgta gaaggcggct tcgtccatct
    11881 gcttgagggt cacgcggaag ccggccttcg cgaccatggc ctgcacgatc tcgctcatct
    11941 tctgccactg ggaaccggtg ctggccacaa gggcgatctc catgtccacc ccgttcgggt
    12001 atccggcctc ggcgagaagc tttttcgctt tctccgggtt gtactcgatc ttttcctggt
    12061 tcgggttgta gtgaatcaag cccctgggca tgacgccgtt ctcaagctgg ccgtgattgt
    12121 agaacatctt ctggaggatc agcttccggt caacggccat ctggaaagcc tttcggacct
    12181 tcacgttatc gaagggcttg atcgcctggt tgaaggtgta gtaatagatg ccgactcgag
    12241 ggccggaaac ggtctgcttg ccccacttgg gatgcgaagt gaagtagggg atctgggaca
    12301 tggcgaaatc acagtcgaag aggtcgattt cacccgattc gaaaagcatc cgcatcgttt
    12361 ccgggtccgt gacgatcttc acgacgaact tgtcgatctg gggacgaccc cggaagtaat
    12421 tggggttggc caccatggtg tggtggtcgt tcaggaccca ttctttcagg atgaaggggc
    12481 cggttccgca ggtcgcctgg gccgtgaggc cgtacttgtt cccgaggggc ctggtgaact
    12541 tctcgttcag gatcgaagcc tgcgggctgg ccatgatcgc cagaaacgga acgaagggct
    12601 tctcaagggt gatccggacc gtgtaccggt cgagcgcctt caggccggcg gtcgaggcgg
    12661 cctttccctc catgcggtcc ttggccccca ggacaaaatc caggatgtcc gtgttcaggg
    12721 ccttggtctt cggatcgagc atccggtcga aggtgtagac cacgtcctgc gcggtcagtt
    12781 cctccccgtt atggaacttg acccccttgc gcaggtagaa ggtgtaaact ttcccgtcct
    12841 tcgaaacttc ccatttcgtt gcgagtccag ggacgagggc ggacttgccg ggggcggtgg
    12901 taacgcattc gatgagccgg tcgtacacat tcatggggat catgtagcag gaagtggtca
    12961 tctgcatgtc ggatgtctgg ggttcttcgt tgtacgcgat ggtgaagact ttttccgccg
    13021 caaaggcccc agcggcaagg atccccgcca ggaggaacga cagcgaaagc acgacaaaaa
    13081 gccttgactt tctcacgcga ctccctcctc tgttcgccaa tgttcgccaa tctcggattc
    13141 ccggttcctt tgaagaagcg attcgagaaa gacctttccg cctgaacggg cctgcttccg
    13201 gtttcccctc ccttcggcct aggcaaggac ttttttgaac acccgcatcg cgttgccgcc
    13261 gaggattccc ctgacctcgt cttcggagta gccccgctgc aaaaggacag ccgcaaaggc
    13321 agggatttcc cggtgccccg ccacaacgtc gtaggtgggg acgctgcatc ccatggtcag
    13381 gtaatctttg aagccgtcgc agaagtcgaa gcccaggcag acgtgttgac tccccaccag
    13441 tttcgcgatg tggtccacgt gatcggcgag atcctccggc cccaggcggc gccggtcgtg
    13501 gtcgggcttg acgaaggcac tgcaggagtt catcccgatc acgcctccgg tcttcgcgat
    13561 ggcaacaatc tggtcgtcgg tgaggttccg catcatgccg gggacgaggg cccggcagtt
    13621 ggaatgcgac gccaccaggg gacccctggc gatcgcggcg acgtcccaga acccctcatc
    13681 gttgagatgg ctgacgtcga tccacatccc gaggcgttcc gcctcttcca cgacctggac
    13741 gccgaagggg gtcaggccgc ctttccggcc ttcgcgcttc ggggcaaaat gacacccgtc
    13801 ggccgcctcg ttcctgcggc tccaggcaag cccgactccc cggactccca gctcgtaaaa
    13861 gacgcgaaga agggacaggt cgttccccag gggctcgacc ccctcaaagg acaggaggat
    13921 ccccgtccgg ccttccgcgg cggcctcttc caattcagag gcgctccggc agatcgaaac
    13981 ccggtctttg aaagcttcct gttctttcag aaggcaggcg atttgatcga gggctttcct
    14041 cagggccatt tcggggagaa aatagctgtg caggaaaagg gaagaaacga ggcccttcac
    14101 cccgccggcc gaaaaaaaac tttcgtattc cggccagatc cggctctcgc cccggtcgta
    14161 gcggtcgcac agatccatgc cgagatcgta atggacatcg atgacaagcg attcgcgggt
    14221 gagcctttcg gctgcctgaa gggtttcggt caagggaaag aacctccttt ggggtcaacg
    14281 agcctttccg ctcatcattg agtagccttt actctaccac tcttttagcc ggtgtcaacg
    14341 gtttaacgag gagacgagga actgttcatg aacaagccat ccattggaat cgaacgtctc
    14401 cgggcgttct tcggactgaa ccaaagcctt tcaggactcc tgttcatggt cattctcgtg
    14461 gggctcggcg aaaagatggc cgaacggttc ctccccctgt acctcctggc ccttggggcc
    14521 gggacccaat ccgtggggtt tctgaacgcc atggacaatc tcctcggcgc tctttattcc
    14581 tttccgggcg gggctctcag cgaccgctgg ggagcgaaaa gggccctgat ggccttcacg
    14641 gccctggcca tggcggggta ctcccttgtc atcctggttc ccgcctggtg ggctgtcctg
    14701 gcgggagccg tgcttttcat cagctggacc gcgatctccc tcccagggat catgagcctc
    14761 gtcagcactg ccgtccccaa aaacaagcgg gtgatgggcg tgaccctcca ttccttcgtc
    14821 aggcgcatcc ccatggcgct gggcccaatc ctgggaggag ccctgatcgc aaggtacggg
    14881 acggtttccg gggttcggat cgcctttacc gccgccctgg tgctgggagg gctctctctc
    14941 ttcatccagt ggaaattcat cgcggacacc cgggagcacg gcaagagccc ccttcacccg
    15001 gccgagtcct ggaaacggtt ttcgccggct ctccgatccc tcctgggatc ggacattctc
    15061 atccgcttcg cggaacagat tccctacgct ttcgtggtgg tctggatcgt tcgggtaagg
    15121 ggcctttccg ccgcccagtt cggcctcctc acggccatcg agatggtcac ggcgatgctc
    15181 gtctacattc cggtcgcggc gatggccgat cggtacggga aaaagccttt cgtgctcatg
    15241 accttcgttt ttttcacggc cttccccctg tccctccttt tttgccactc cttttccgcc
    15301 ttcgccctgg cttttgtcct gagagggctg aaagaattcg gggagcctac ccggaaagcc
    15361 ctgatcatgg acctggcccc cgaggacgcg aaggcttcca cgttcgggac ctactacctc
    15421 atccgggacg tgatcgtttc tgcggcggcc ctgtccagcg caacgctttg gaacatttct
    15481 ccagcggtga acttcctgac cgcatttggt ttcgggctgg caggaacctt gctttttgcg
    15541 gccttcggga aggacatccc gcaaggggcc ggcacttccc ccctttcccc gaagtgaacc
    15601 ggggagtatc ataaaaaaag agttcgcccg tttctgcatg catcgctacg gcacggcttg
    15661 aacgcgatgc aaggggaggg aaaagccggg atgtcggcaa aagcagtccc cactcgaggc
    15721 aatctcgtcc gcctggcccg ctccctcgaa cttgcgagga aagggcatga cctgctcgag
    15781 cagaagcgcc aggtcctgat gatggaactg acggcgacga tcgcgaaggc cagggacctc
    15841 cagaaagaag tggccgcggt ctttgaggag gcctacgcgg ccctgcagcg ggccaacgta
    15901 tcgctcggca ttgaaagcgt cgaggagatt gcccacgcga tccccgagga aaaaaacttc
    15961 acgatccgtc tccattccgt catgggagtg gaaatcccgg aagtggaccc gatcgagccg
    16021 aagcggggtc ccgccttttc cttctgggat acctcgggaa gcatggacgt cgccgctctt
    16081 gccttccgca aagtgcttgc ggctcttgcc aggcttgccg aggtggaaaa cgccgtctac
    16141 cgcctggcgg ttcagatccg aaggacccac cggcgggtga acgcgttgga gaaagtcgtc
    16201 attcccacga ccctttccac cctggcttcc atcgcttccg ttctggaaga gggagaaagg
    16261 gaagattttg tgcgcatgaa aaccgcgaaa aagagccgcg gggagagggg gggctaagga
    16321 gtgtcatcgt tgcaggaagc cctcgtggtg ttgctcggcg ccgagagcga aggagtccgg
    16381 gaagtacagg aggccaaaat ccaggccgag gccctgctga aagaaaccag ggaacggatg
    16441 gcccgggagg ccgagacccg gatccaggca gcccggaaaa aggccagcgc cgttttggaa
    16501 aacgcacggg ctgccgccga cgaggaggca cggtccgtca ccgagcaggg caaatcggaa
    16561 agggagcgga tggagcggcg gttccgtgaa atcgccggag gggtggtcga ctccctggtc
    16621 caggaggctg tggaaaccct gctcgggaag agaggcgggc cgtcgtgacc ttcttttttt
    16681 cgctggggga gaggacggca gccggagcga aagcccacgt cctcttctcg aagctgctga
    16741 aggacgaaga aatgtgggcc ctgctgcaat gcgacgacgt cccggagatc ttctcccaac
    16801 tggtgaggag cgaaggctac ggggatgccc tctcttctct cgttccccag gaggtgaccc
    16861 gcgttcagct ggaagatcgc ctcaagcttc tcctgccgaa acgggcggcc cagttccttc
    16921 cttacctgac gggagcccgg cgccgctttc tcctggcctg gatggaacgg gaccaggccg
    16981 gggccctgaa aacggccctg cgcttcgtcc tgacccggca cggagaccgg gaagccatac
    17041 gggcgcggat cgcggccctt ccccgcacct ccctgccgtt cgaccttctt ctctccagcc
    17101 gggacttcca ggatttctcc gatgccctgc gggggacccc ctactttcac cccctgcagg
    17161 aacccctgaa gcggctggcc ctgggggaac agaacctgtt ttccgccgaa gcggcgatcg
    17221 accgggaggt gctggtccgg ctcctcgagg ccgccaaagc cctttccgcg ggagagatgc
    17281 gctccgttat gcgcctgctg ggagttcgct tcgatctcct gaacatctcc tggacctacc
    17341 gttcgctgcg atactacgat ctaacccctg aagaacgggt cgccaggctt ctgcctttcc
    17401 gctaccggct gcacggggat tctctgcgga agatggcccg agcgggcgaa ccggacgagt
    17461 tctggacgat cctgaaaacc acggcctacc gagaggcctt cggcgaatcg ccgccgatcg
    17521 aggaaatggt catggacagg gatgtgcgaa gatacatccg ccagacagcc tacaaggtct
    17581 tccggcaggg ccgtccggat ctatcgactg tttttgccta cctgaccctg ctggaatccg
    17641 agatccggga cctggccacc ctgatcgagg acgtgcggta cgactacgac cggaggcacg
    17701 cggccctctt cctggcccgg cccctcattc cgggaggcga agtgcgatgg cagtggtaaa
    17761 aatgcttgcc gtaaccctga tcggccccaa ggccgagatg gaaaccgtcg cgcgcctgat
    17821 ggtcctgacc ggagggttcc agcccctgcc cctggacctt cttttaaagg accggtcggt
    17881 tcggtcaaag atcaccactg cctcggaaaa cccctacgac gaactgctcg tcaagctttc
    17941 ggaggtctgg aaagctgccg gggaacgggt gccggacccg tttcccgtgc ccatcacccc
    18001 ggatttcagc ctggaccggg cggcgaaggc cgtcaacgaa acggttcacc ggctgtcgct
    18061 ctggtcgaag cgccgcgaag agctgtcgga agagcgggag gaactggaag cggcgcaaat
    18121 cctgacggcg tccctttcgg aaagggggat gaacctcgcc gacctcgccg acaccaccta
    18181 cctggttccc ctgttcggga aactgacgga tgagaactac cgccggctgg aggaagccac
    18241 cctcgaagcc ccgctcctgc tggaacccct gctttcgagg catggattca cctggtttct
    18301 gggattgacg gccccaggct accgcgaggg ggcgaagaag ctcctggaaa gcctttattt
    18361 caaaggattc tccctgaaag agatggcatc ctccctcgcg ggcgatccgc aggtgcagat
    18421 ctccaggagg atcgaaaaca cccggaaggc gatcgagggc ctgaaacggg cagccgcgga
    18481 ggttctccaa tccaaccgcc agggcatgga gcggctctat gcttcgatct acaccatgca
    18541 gcgcgtctac gatctctgcc gcggccgggg ggagctgagc gggctttacc ttctgtccgg
    18601 ctggattccc gaggacaccc tgcagaggac cctggaaatt cttgaaagag aggccccgaa
    18661 aacaacggtt cacatcgaag agctcgggga gatgccctac accggcatcc gggttcccac
    18721 tctccttcgg aacctgcccc tggttcgcgc tttccaggac gtggtttcgc tctatagcct
    18781 ccctgcctac ggggagatgg atccgtcctt tttcgtggcg gtcagcttct gcctgttctt
    18841 cggcttcatg ttcggggacg tggggcacgg gctcgccctt atcgccgcgg caacgatcct
    18901 ccagcgcaag cggctgctga gccggtcaat ggggacggtt ctcaagtctg ccggagccgc
    18961 ctcgatgctt ttcggcttcc tgtacgggag cgtcttcggc gtcgaaggct ggatcccggc
    19021 tctctggatt tcgcccatgg aggacatgaa ccggcttttg ggggtttcga tcggggtggg
    19081 ggtttcggtc atcacgctcg ggatgatcct caacatgatc tcctgctaca aggcccggga
    19141 tttcgggagg ctgctcttcg acggcaaggg cctggccggc atggtcctct acctgtctat
    19201 ggcagcgatt ggctatgccg tcctgacggg acagcgaatg ccattccctt cctgggttcc
    19261 atggaccttc ctttcggccc tgctgaccgt catcctgatg agggatttcc tggcgcgcct
    19321 gcttctccgc cagagaacgc acgaagaggg gggactgtac gccttcgagg tgttgcacaa
    19381 tctcctgaac ttcctgagca acacggcctc tttcgtccgc ctggcggcat ttgccctgaa
    19441 ccacgtggga ctctcccttg ccgtcatgat gctctcggac atggtccggg agctgccggg
    19501 agggatcctt ctgaaggggg tcctccttct ggcaggcaat gccgtcatca tcgcgctcga
    19561 gggattgatc gtcttcatcc agaccctgcg cctggagtac tacgagttct tcagcaagtt
    19621 ctaccgcgga gggggaaggg ccttcaggcc ggtttccttc agggatcccc ggcttcaggc
    19681 tggatccccc acgcccggaa gctaagatct tcaaaagggg aggatttcat gttcggattg
    19741 ctcgttctta tcatcgcttc gctgaccttc ggactgctcg gtgcccgcct ttccgggcgg
    19801 gcggtaaaga atccccgggg agttctcgct gcgagccttt tcgcctgttc cgtcagcctc
    19861 ctggcgggaa gcctgctggc gctctgcacc ggtcctgccg cagcggctga agcgggggcc
    19921 accctgggag ccggggcggg gatgggattc atcggtgcgg ccgtttccac cgggctggcc
    19981 tgcctgggcg ccgggatcgc cgtggcggtc gttggagccg cggccctggg gatcatcggc
    20041 gaaaagccag agatgctggg gaccaccctg atttacctgg gcctggcaga agggatcgcc
    20101 atttacgggg tcatcgtggc cctgctgatc ctcggaaaga tctgagggac gatgcgcgcc
    20161 tacctgctca gcgacaacct ggacagcctc gttggccttc gcctggccgg gatcgaggga
    20221 cggattgccc gcggcgccga ggaatgccgg aaagcgattg aagaagtgct ggaggatccg
    20281 gaaatcggga ttctcgttct cacggaaaag gttgccgaaa cggtgccgga cctcgtccag
    20341 ggtctcaggg agagaggcga gcttcccctc gtggtggaga tcccggaccg ccacgggatg
    20401 cgccgcgccg ccgacttttt gaccgcctac gtcagggacg cgatcggggt gaacctcgaa
    20461 tgaacgaaat ccagaacgag aaactgaacg gcctgaggga actccttctt ctccaggccg
    20521 agcaggaaaa aatgggcatc ctggaaaaag ccaaaaaaga ggcagaaagc tggatcgccc
    20581 aggaagaaga gcggatctcg cgggaagagg agctgatcct gaaagacgca gcggaccggg
    20641 cggaagccac gcgcaggcgc caggtcatgg ccgcggaaag ggaaaaggct gcggaggggc
    20701 tgcgcctgca gaaccggctg ctctccgaag ccgtgtcgat gttggaagac cgattctgcc
    20761 gcctgcggga gcgaaacgac cacgttcgaa tcctggcggg cctcatcgcg gaagccgaat
    20821 ccgccctgcc cgagagcggc ggcctccggt tcaggctggc ggggaccgac gctccgatcg
    20881 gagaagccgt tgcgaaggaa gccgaagcgc ttctgcccgg catcgaggtc cgatttgacc
    20941 cggatccggc cccgatcctg ggcggggcct ggatctggac cgccgacggg cgaaggtttg
    21001 tcgcggcgga ttggcgcaac cgggctcagg agatggcggg cacgctagcg gaacgcctgc
    21061 tgccgatgct ttagacaccg gaccagggag gggaaggcat cgtggaaacg ttcggaacca
    21121 tcgtccgtgt caacggcccc gtcgtccggg caagaggcat gcggtccttc ggaatgagag
    21181 aaatggtccg cgtcgggaaa cttggcctat ggggagagat catccgcatg gaaggggacg
    21241 aagccctcat ccaggtctat gaagacaccc agggcctccg tctcggtgaa ccggtcgagg
    21301 gaacggggga acccctctcg gtctccctgg ggccgggtct catcggtcaa atcttcgacg
    21361 ggatcgggcg ccccctcggc gctcttttcg aaagggaagg cccccttctt tcccggggga
    21421 tccaggtctc catgatcgac ccccagcgct catgggaatt ttcgccttca gcgcggaaag
    21481 gagacgtcgt caccgcaggc atggtcctgg gaaccatcca ggaaacgccc cttttcgagc
    21541 accggatcct tgtgccgccg gacctggagg gggaaatcac ggacatccgg accccgggca
    21601 cgattcgagg ggaagacgtt gttgcccgta tccgggacgc aaggggacgg acggtccagg
    21661 tgcctgcaat ccagcgctgg ccagtccggg ctgcccgccc ctaccgggag aggctgctgc
    21721 cgaccgagcc gttggtcacc ggccaaaggg tcatcgacgg cctcttcccg atcgccaagg
    21781 gggggacagc ggcgatcccc ggcggcttcg ggacgggaaa gaccgtgact cagcaccagc
    21841 tggcgaagtg gagcgaagcc caggtggtcg tttacatcgg ctgcggggag cgcggcaacg
    21901 agatgaccca ggttctcgag gaattccccg tgctggagga ccctcgctcg gggcgcccgc
    21961 tcatggaaag aacaatcctg atcgcgaaca cctcgaacat gcccgttgcc gcccgtgaag
    22021 cttccatcta caccggcatc accatcgccg agtattaccg ggatatgggc tacgacgtgg
    22081 ccctcatggc cgattcgact tcccggtggg cggaagctct ccgtgaaatc tccggccgcc
    22141 tggaggagat tcccgccgag gagggcttcc ccgcctacct gcctacgagg ctggcggagt
    22201 tctacgaacg cgcggggcgc gtcgtcaccc tgggagggaa cgagggcagc atcacgatca
    22261 tcggagccgt gtccccccct ggaggcgact tcaccgaacc ggtcacaagg aacaccaagc
    22321 ggttcatccg gtgcttctgg gcattggacc gcaaccttgc caatgcccgg cacttcccgg
    22381 ccatcgggtg gctcgaatcc tacagcgagt atgcccagga ggttgaaaac tggttcggga
    22441 ctcacgtgga ttccaggtgg gggcagcttc gggagcgggc ccgggccttg ctggcggagg
    22501 acgaaaagat ccagcaggtg atccgcctcg tgggcgagga cgtgctgccg gacgaacaac
    22561 ggctgatcgc cttcacggct tttttgatca agaacggcta cctgcagcag ggagccttcg
    22621 gggaagactc cttctcgccc ccggaaaaag ggttcgccat cctggagttg atgctccact
    22681 tccacgacaa ggccggggaa ctggtcaagc ggggagtccc cctttccctg atccgggacg
    22741 gacgtcccgt cctggcgctg acgcacctga gggaacgtcc gctgaatgac ccaaaggggg
    22801 ggccggccgc gctccgcaag gagctcgacg ccttcctcga aaaggtcggc acggaacgtt
    22861 ccgccgcgaa aggaaggatg tagcgatggc catcgccgag tacatcggtc ttgaaacgat
    22921 caacggtccc ttcgtcctgg tcgaaggggt ccagggggcg ggctacgggg agctgaccga
    22981 cgtcatcggt ccggacggga aggtccgaaa agggcgcgtg gtggtcctgg acgagcgggc
    23041 caccctggtg caggttttct cggggaccgg ggatctcgtg cccgaatcaa cccgggtccg
    23101 cttcctggga aaggaactct cgatgacgct tgaccccgcg atcctcgggc ggaccttcac
    23161 cgggctggga gaaccgcggg acggctgcgg gcccgtttac ggcagggtca cccggaacgt
    23221 gaacggccag ccgatcaacc cgatggccag ggcctaccca agggacttca ttcacacggg
    23281 gatctcctcg atcgatgtcc tcaccaccct gatccgcggc cagaaactgc ccatcttctc
    23341 agggaacggc cttccccaca atgccctggc ggtccagatc gcgacccaat ccaggctggt
    23401 gggggcagaa aactttgcgg tcgtcttcgc ggggatcggc atcaagcacg acgacgccgc
    23461 cttcttctcc cgcgaactgt ctgacaaggg acgcaccgat aagatcgtga cctttctcaa
    23521 cctggcggac gatcccgtga tcgaacggat cgcaaccccg cggttcgccc tgacggccgc
    23581 ggagtacctg gccttcgacc tcgggcttca cgttctggtg atcctgacgg acatgacgaa
    23641 ttattgcgag gcgttacggg aactgggtgt ggcagtaggg gaggtcccga gccggaaagg
    23701 gtacccgggc tacctctaca gcgacctggc ctccctttac gagcgcgccg gggtcctgag
    23761 agagggaaag gggagtgtaa cccagcttcc gatcctgacc atgcccaacg acgacatcac
    23821 ccaccccatc ccggacctga cgggcttcat caccgagggg cagatcgtcc tttcccggga
    23881 actggacgcc aagggaatct acccgcccat cgacgtcctg gcgagcctct cccgcctcat
    23941 gaaagacggc atcgggaaag ggttcacccg ggaggaccat cccaacctct caagccagct
    24001 gttcgcctcc gtcagccggg tgcaggaagt gcgtgccctg gcgagcgtgg tgggatccga
    24061 cgagctggga aaacaggaca aggcctccct ggcgttcgga gagcttttcg agaaccagtt
    24121 cctcagccag ggaaccggcg aggatcgcga catcgggttt tctctcgaca aggggtggga
    24181 gatcctttcc gtcctcccca gggagcagct gacacggatc tccacggaag aagcgaggaa
    24241 atacctggca caaaaatgag aagcgcgagg cgaggtccaa cggcctcgcc tcgcgcttct
    24301 ttaatccgca accctgggtc cgggagtcaa aagggtaaac aaatccttcc ctactccgca
    24361 ttcggggcag atccaatctt ccggcaaatc actgaaaggc atgggaggca ggattcctgc
    24421 cttggggtct cccacctggg gatcatagac atagccacac acggtgcaaa cgtatttcgt
    24481 gtgcaacggg gacatcattt ctttcccccc cttctggagt gattgaactt cccaatgctt
    24541 ttctaaagcc ccaactcccg cagtttttct ttcccgggga tcccttccgg cgtccaaccc
    24601 ctgattcggt agtaatccca gaggattttc cccatgtaag ggatactgcc ctttgccgca
    24661 ccgctctctt tatcgtgcac tcccaagcga ggaggcagag cgtcatcttt tctggtaatt
    24721 ccaattttac cattaaacaa tctttttagg ttaaagttcc tttccccggc tttcatcagc
    24781 tccgctttcg aatagggaat cccggttacc tggtgaatcc actgggcaaa aatcgccggg
    24841 ctcgtcgagt ctttcccccg gatgaggaac ttgcaaagcc ccaggttgtt catcgcttcc
    24901 atgaaattct gcatgatcac tgcgatttcc gccttgtttt cgtgtccgtg acgggtgaat
    24961 tctttctcga aatcaacgag cgagagggga aaggctccct gttccgcgaa gtaggtcagg
    25021 ccttccaggt ggcaggcgcc ccgattggcc gtcgcatagt tggccgccat ggacgtaaat
    25081 gcccggggat cgtggaaggc gtactccagt cccttcgtgt gcacggcaaa ttcgaccgcg
    25141 agcccgccca acttttctgc ggccctcttc actccctgcc cgaggaattc cccgaatccc
    25201 tccctcttgc cgatgagttc cgtcagcttc aggatcgcct ccccgttccc ccacgtgatt
    25261 tcgattccgc ccgtctcttc tttcgtgatc agcccatttt cataacactc catggcccag
    25321 ccgatcacgc cagaggtcga aatcgtgtcc aacccgtaac ggttgcagag gtcgtttgcc
    25381 gccacgatca catccagatc ctcattgagg aggtttgccc caaaaccgtc cgtcgtttca
    25441 tactcgggcc catgcgccag ttgccccttg aacggaccga ccggaaccct gacatttttc
    25501 ccgcagcgaa tcgggcacgc gaaacaaccg taatgggaaa cgaaaaccgt ttctgcgatt
    25561 ttctgcccat tggtcttcgc ggccccctcc gtccaggacc caagggacca gttccggatc
    25621 gaaagatctc cgtttgcctc tacggccatc actccgcctg cggtaccgaa gtccgtcaat
    25681 cccttcgtgt tggccttgat caccgggacg gtctcctgaa tcgattgaac cagcccgggg
    25741 cggtcgaaaa gatccaccgg gtttgtccca cggatgacca ccgccttcag gttcttcgag
    25801 cccatcaccg ccccgagccc cgtcctgccg gccgctcgag tctcgctgcc gccaaacatg
    25861 atcccggcga gcaaaacgag cttttctcca gaaggtccta tcgcagccac ctcgtacttc
    25921 tccccgtgcc tgtccttcag aagttccgaa gtcttgtagg tgtccagtcc ccacaggtct
    25981 tcagcctcgc ggatcgaaac ttgcccgttt tcgatcaaca ggtaggaagg acgctgggct
    26041 ttcccgcgaa ccaggaatcc ctcgtaaccc gtcttcctca attcggctcc gaaataccca
    26101 ccaaccgtcg cctctcccca aatccctgta aggggggatt tcgcacaaac ggccatcttg
    26161 catccgcagg gaacaggggt tcccgtgaaa agcccgttca tgaaaacgag gatgttttca
    26221 gggccaagag ggtccaccgc ataggttccc tcatcgtaga gcagcctggc cgcgactccc
    26281 gaccctccca ggtgcagttg ccagtactcc tccgggagct gtttttcctg gatggaaccc
    26341 tttgtcagat cgatgtcaaa aactttcgcc gagatgcacc tcattcgcct tcccccctcc
    26401 tgctttgctg caggaggggg cttcgacaag ccccctcctg gcttctttga tccgtttaat
    26461 aggctttctt gtaaagttcg acgatctggg cttccgtcac gaacctcggg ttgtttccag
    26521 ggctgccgct gaccacggcg tcatgcgcca ttttctccag gggcttctca aaggcctccc
    26581 tgtctggaac aagggttcga atcggaggaa cctccacgtc cctgcagagc ctcttgatcg
    26641 cctccacccc tttttccgcc gcctcgaggg aagaaagccc cgcgaccttc tctcccattg
    26701 cctccgcaat cagcgcgaat ttttcaggct tggcaatgta ggtgaactcg gcccaaaccg
    26761 gaagcaggac cgcgttggaa atgccgtgag ggacgtggaa caccgccccg atgggccttg
    26821 acatcccatg gatcagggtg acggatgcgt tgttgaaagc cagccccgcc tgtgtcgaag
    26881 ccagcatcat ctgggctctc gcttcgagat catctccgtt gcaccaggct ttccgcagat
    26941 aggtcccgat gagcttgatc gcgttgagcg ccaaaagatc cgtcatcggc tgctcctttt
    27001 ttgaaacaag ggcttccaca gcgtggcaga acgcatccag ccccgtagca gccgtcacct
    27061 ggggcggaac cgaaagcgtg agaaccgggt cagcgaccgc gatcatggga atgacaaaag
    27121 gagagccgat gagcatcttg acattgttct tcgtgtcagc aatgatcgtg aacttcgtta
    27181 cttccgatcc cgtcccggat gttgtggcaa ttgccaccac gggaggggtg ggaacggcca
    27241 ccttgtcggc tcccatgtaa tccgtgatct ggcccccgtt ggtggcgaga attccaacag
    27301 cctttgcggt atcgatcggg cttcccccgc ccagtccgac aaggaaatcg cattcacttt
    27361 ccctgtagac tttcaggcct tcttcgacgt agacgtctgt cggttcggtt ccaaccccgt
    27421 caaacagaac gctttcgacc tgaacgtccg cgaggatttt ttcgacctga gccgcatacc
    27481 ctgctttgac cataattttg tcggtcacca gcaaggcttt ctttttcccg agcctcttta
    27541 cttctttccc gatgtttgca aggcatccag gtccctggag caatacctga ggcatccgaa
    27601 actccgttgg ccgcatcatc tgtttgaacc ctccctcttc tgtaatgttg tgtgttgctt
    27661 gcggttggga actcttgctt taggcttgaa cgattcctag ggcaatccca tcgcgtggta
    27721 gagaatcaag gccgcgacgg caatcatggg gacaaaaatc tgggtcacgg cgatgggcca
    27781 gtaggcttcc ctgtgagtct ggtgacaaat cgccagcaag gtgatctgcg ccccctggtg
    27841 aggcagggtg tccaatgtcc ctgccgcaat gaccgaaatc cggtgaaccc agggaagggg
    27901 aacacccatg ccgacaaacg tgtccttcag ggccgcaaaa gccacgctta gcccgccgga
    27961 tgcagaaccg gcagctccgg cagctaccgc gacgaccgtt gccacaaaca ggagcggggg
    28021 catgttcagc cccttcagcc cgtcgatcag ggtcgtgaag ccgggagtct ggcggaccac
    28081 tcctgcgaac ccgacgacga tcgcggtgtt gagaatcgct ggcgccgagt tcatggcccc
    28141 gttgttgaaa atggacaccc attgctggta tccccctccg tatttcatca ggaggagaaa
    28201 gcccgcaaga atgccgaaaa ggaccgcggc ttcaactttt acgtggaaca cgttgaacag
    28261 caccaggatc aaaatgcagg gaatgattga aatgaccggg ttgggaagtt tttgcgctac
    28321 atcgagcgaa gaagtgaatt tccggacgtg ttcgtcctcg acaaaagtga agcctttccg
    28381 cgcgtactgt ctcccctgcc attcgaggta gataaaaatc aggacgaatt ggaggagagt
    28441 ggtcatcgtt ccgccgatgg ggtccgccaa gggactcgtc ccgagaaacc tcatcggaat
    28501 gacgttttga atcgccggcg ttccgggccc gctcatcgac caggtccagc accctgcaga
    28561 gatcgtcgca gggatgaggc ggcgggaaag gttcgcttcc ttgaacatgt gcagggcgac
    28621 cgggtacatg gcgaagaaaa tgacaaaccc gctgatgccg ccatacgtca ggataccggt
    28681 taccgtcatg atcagcggcg caacgtactt ccccttggtc aggctcgtca tccactgggc
    28741 aagagatttg gcagcccccg tttcttccat cactgagccg aaaacggacc cgacgaagaa
    28801 gacaaggaag aaactcttga tataggctgc ggccgtctcc atgtagggac ccagcatcgt
    28861 atccatcaga ggaagccccg ccacagcggc aacaagcaaa gcgagcaagg ggcctaggat
    28921 tagtgcgctc catttctgga aagccaatac cccgaaaagg atcagggcaa tcggcaggat
    28981 tgccacctgg agaaaatcca tcttcatccc ccctctacca tctctgaagt ctggaaaccg
    29041 ctattcttct tttgcctgtt ttccgggttt tccgcttcta tccccccccg atgatgggca
    29101 tgatgtccag ttttccgccc ctttccagcg attccgaaag agcaacccat ttgccgtcca
    29161 aaaacgcacc ttctacttca gacacaggaa ttttgagttg tgagagaatc tccccgaggg
    29221 tgacgggcgc ctcgacatcc aagacaacct cttcccgctt ttcgctctgg tagtagctga
    29281 ggtgcccctt caaatgaatg ttgatccgca aaacccgtac ttcctcctct tcatagaaaa
    29341 attcttccct gtttaaatta agagaaagca agcccgaagg gtatgtaaat gactgataag
    29401 gtcgcagcct ttttggtgtc ctggatagac aatttcgggg cctcgtcaat tcactgaaag
    29461 gcaatgggac ttgaggaaaa gggaggtata ctgttgttgc ataaagagac catttcactc
    29521 gctgggagct tttgatgatc gtcgctgacc tgcttcatca gcatatcttc ccggactttc
    29581 ggctcgtcgc ggggaatgaa gggcttacaa gggaaatcac cgcgatttcg gtcatcgatg
    29641 cccctgacat ggcaagctgg ctcaaggggg gagagttcct tttcggcacg gggtatattt
    29701 tcaaggattc ccagtctgcc ctctcccttt tcttccacga ggtcgcgggg cggggcattg
    29761 cggcgatggg gctcaagctg gaccgctacc acgcgaaatt ccccgaaacg ctgctccagg
    29821 aagccaacga actgaaaatc cccgtctttg aaattccttt tcattaccgc tggtcggaca
    29881 tcattcacga aatccagtcc aggctgtacc accaggacca ggaaaggctc ggccagagcc
    29941 gatacgcccc acccctccta tggagttccg attgggattc aaaaagactg atttcaaccc
    30001 ttgccacgga aatccaaaag gagatccttg ttgaaattca tacccttcag atgaaatacg
    30061 ggttcgcccc ggacggctcc ccgattgaaa gcgatcgatt caacgaggta aaaacggctt
    30121 tcgccgttcg ggaaatcgaa ctgcccagaa aggggaacat ttatatctcg ctggaagaaa
    30181 aagaagtgca ggaggggaaa ctctggacag ccaattacag tcttaagacc gaaacaccga
    30241 ttacccttac cttgatcttg aacaaggaag aaaaccaccc ttccctgagg gacgaaaggc
    30301 ttgtcctgcg tgcgatcagc ctgcttcgaa gcgaggccct ggagctcgcg ctggcctcga
    30361 accagctttc ggccaaaaga gaacgattcc ttgaaaatct ctgcctgggc agttaccaaa
    30421 atcaggacat ggcggctcaa cgggcaaaaa acctgggcgt ttcaattccg ttcccctgcc
    30481 aggtttttgg cgtggcaacg atggagcgga tcaccctccc tctctggatg cccccctatg
    30541 aaaaccgcta cgccctggga aacttttggg tggttatcga atcctcaaaa aaactggagc
    30601 aggatgccga aacgctccac gaaatcgccc gggacaacca tttgtggatc gccatgggaa
    30661 gtgttgcgga tgattttcca ggtatccagc gttcttacga agaaaccaaa aaagctctgt
    30721 actggatcag ggatttcaag ccaaggccag gcctttaccg tttctcggac cttgccctgc
    30781 atgccttgct cggacggctg tcaagaatgc ccgaatcgga aggcctttgg aaacgtttct
    30841 ggatgccgct caagaccatc gattcaagcc gaagggcaat ccccctggag gaagtgctga
    30901 acagcctcat cctgtgcgac tttaacgcaa aaaaatcggc ggatttgctg catattcact
    30961 acaacaccgc tcgcaactac cttcgcgaga tcgaagaact cctgcgggtc gacctgaata
    31021 acaggctgca tcgccttgcc ctttccctgg ctcaccaaat cgagattgcc aagtcgaagg
    31081 aaagctgacc gcctttttac ctcttgtagg gaattctcct cgaaggctcg aacggagcct
    31141 tcccttcctt caggatccat tcccggaaga cttcgaggtt tcgatcttcc atgtgatcga
    31201 acaacatcgg cgtgcgggtc ccatgtgcat gaaaccgcat gggatcggga tccagccggg
    31261 cggaaacgat ttcctcgttg ccgctggcct gggccaggag ccagcctgtc gggtcaatga
    31321 tcatcgaact tccgaagaag ttgttgccgg aagcgtcctg ccccaccgca ttgaccgcaa
    31381 catagtagac gtggctgtcg tacgctctcg caaaattcgt aaacgcccag acgttgtagg
    31441 aacgcaggag ggaagaaggt cgaacgatga cttccgctcc catcagggtc tcgacaattg
    31501 aaaattcggg gaaatcgcca tcgtagcaaa gagaaaggcc gatcttagcg aaaggggttt
    31561 ccacgacgac cgccttgttc cccgcggttg tccatcctcc cccttccttt ctttctgtcg
    31621 ggaacggatg ggttttatgg tagacgtcgc ccaggatctt tccgtcactt ccaatcgcga
    31681 ccacagagtt atacacgatg tcattttcgt ttcccctgga ataggtgggc catatgacgg
    31741 agaccccgta cctcgccgct gcctcctgga ttggaagggt ggttttgccg ggaatgcaat
    31801 ccaccgcctc ccacagggca gttacggttt cttcctgtgt cggtctgggg ggaggggtaa
    31861 acccggttgt aatcgcttcc gggaaaacga ccaggtccgc cccgtgttca tgaacagccc
    31921 tttccaggaa aacaatcgcc ttccggatgt tggcttcaac gtccatggga gttacagcaa
    31981 actggcatcc tgcgacgaca aacgatttca ttttttccct ctcctatcct atccctttcc
    32041 agcctgtcat ccatgaaaaa tggaggactc ctcttctctc gctatcggcc aaatgggtta
    32101 gcgttcccct tcccgtctat cctactcgtt tctacgcaca gccctggaag ctgtcaagcg
    32161 ggtccagaac ctggacccgc tcttttgctt cctggatttc gccttcaccc ttacgcggtg
    32221 aggagggtga gcatggcacc ggcggccacg gccgtgccga taacc
//
LOCUS       DAOOVN010000011        30261 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_1028_length_30261_cov_14.101569, whole genome shotgun
            sequence.
ACCESSION   DAOOVN010000011 DAOOVN010000000
VERSION     DAOOVN010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 30261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30261
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1028_length_30261_cov_14.101569"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            269..961
                     /locus_tag="PLY83_02390"
     CDS             269..961
                     /locus_tag="PLY83_02390"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HOV28916.1"
                     /translation="MGAWRTLVEDFKAVKERDPAIPSGIRGFFEVVLCTPGFQAVAAH
                     RLIHFLHVRMRIPVLPRFLGLLVRWWTGTEIHPGARLGKGVFIDHAAGVVIGESAIVG
                     DNVTLFQGVTLGATGNEKTWKRHPTLESGVFVGSGARILGPVTVGKNARIGAGSVVLK
                     DVPPETTVVGVPARVVKSGGRRVPSPLERAESRIEALEGMVRDLQEEVQRLREASAFY
                     GPSKFKAGKGVA"
     gene            963..1889
                     /gene="cysK"
                     /locus_tag="PLY83_02395"
     CDS             963..1889
                     /gene="cysK"
                     /locus_tag="PLY83_02395"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOV28917.1"
                     /translation="MSFRVDSLVCFSLVGNTPMARIEAGGGTEIWIKMEGNNPGGSIK
                     DRAAWGMLRKAEEEGLLQEGAVIVEPTSGNTGIALAMLGRSLGLRVILTMPESMSAER
                     RAVLAAYGAELILTPAAEGMEGSVRKAEEVTRELNGAFMPGQFDNPGNPWSHQVTTGP
                     EILKALKGKAPFAFVAGVGTGGTLTGVGRSLKSAFPDVRIIAVEPAKSPLLGGGRSGA
                     HGIQGIGANFIPKNLDRSLVDQVEPVTDEAAFDMTRWLAREKGLFCGISTGANVAAAF
                     SAAAKMPSPGIVVTVSPDRGDRYLSTGVFGRF"
     gene            complement(1892..3343)
                     /locus_tag="PLY83_02400"
     CDS             complement(1892..3343)
                     /locus_tag="PLY83_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOV28918.1"
                     /translation="MRPAIVAKVLSLVTTVVSLFLLWGMAWAAWDRSSDLAALGASLL
                     IGLSISGILFFLGRNASAKDLGPREAFAVVSLSWILCSAIGALPFYLQGSVSTYTDAF
                     FEAMSGFTTTGASVLTNIEANPRGILFWRSLTHWLGGMGIIVLSLAILPFLGVGGMQL
                     FKAEVPGPVPEKLTPRIQQTALLLWGVYVILSLTETVLLVLAGMNLFDALTHTFGTMA
                     TGGFSPKNLSVGFYRSPFVDWIITVFMFLAGANFTLHYFALRGRLSVFWKDEEFRFYS
                     WVTLIGTATVMVILLSGNAYDSLGEALRFSAFQVVSILTTTGFVTADFEKWPAYTQLL
                     LLVLMFVGGCAGSTGGGMKNIRILLLARQAGSEIRRLLHPRAVIVPRVGGKVVEREAI
                     NSVTVFFIVYMALFVGASLVMTALGLDLVSGISSVAATLGNIGPGLGIVGPMDNYAAI
                     PAAGKWVLSLCMLLGRLELFTVLMLFVPGTWRK"
     gene            complement(3347..4711)
                     /gene="trkA"
                     /locus_tag="PLY83_02405"
     CDS             complement(3347..4711)
                     /gene="trkA"
                     /locus_tag="PLY83_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOV28919.1"
                     /translation="MRIILVGAGEVGQNVARELIRDGHDLTIVERDEDRAQQIDEELD
                     ARVVRGNGARPQVLEEAGLGEDGGVDLLIGATDRDEVNILACWIAKRSGVPQTMARVR
                     SIEFTDTQSWARDLNVDLLISPERSVAREIQELLQVGASFHAATLMQERAGIYGFTLS
                     GGSPLLGRPLKDLTREYQKLRTIIVYVRRGRSGFVPSGESVLLEGDLCYVATLREERD
                     RVAGLFTGKSARPLRRVIIVGGGKIGFQVAKRLEENFQKVDIRLIDRDLEKCEILARE
                     LEKTTVLHGDATDSDLLRYEGIEETDGFVCTTANDEMNLVLSAVARNLGARKTIAVIR
                     KSSYLELQEAFHADALVNPNEALASTILHRIRYPRGMGAVSVIDQIDAEMGEITLTPD
                     SPAAGKKVMDLELPKGVILALVERGAEVFVPKGDTVLKENDHLLFFSSRDHLHKALDT
                     LGAD"
     gene            complement(4716..5711)
                     /locus_tag="PLY83_02410"
     CDS             complement(4716..5711)
                     /locus_tag="PLY83_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HOV28920.1"
                     /translation="MERNPVIVVSFPADSEARSLLYRELGESSRLVFLKDLGDIESRR
                     SALESADVLLSFILGREIPSPELLFLKNLKLFQSLLAGVDALPFPLMPKQAILCSNAG
                     AWADPLAEHALAMALALGKNLLPLHAKLARGEFDNSRESLWFRKASAAIVGFGGIGKA
                     VARLFRSLGMEIRAVNTTGQTLEPVDWIGTLDQLEEAVRDAHVVVLSLPLTRRTRGMI
                     GKKQLEWMRKDAILVNVARGALIQEEALYEHLKANPSFRAGVDTWWIEPATHGEFRVH
                     FPFFRLENFLGSPHNSNLVNGIFPVALQAAVDNVCRFLAGEPPLGIVDPADYMED"
     gene            5808..6386
                     /locus_tag="PLY83_02415"
     CDS             5808..6386
                     /locus_tag="PLY83_02415"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HOV28921.1"
                     /translation="MKGRFCVKAAGRILFTAALAIVLSAVAASAAEFSADMVMKGPDG
                     QAMSGKVFVKGQKIRQEIQQDGERVITILRMDKKLSWVLMVDEKIYMEMKMQKSAEDP
                     SLVEKTDPAKIRRLGKETVNGFVCEKIQVTEKDAVITQWISEDLGWPIKGEAKTPEGV
                     SSHEYKNIRKGNVPDDLFEVPKGFQKMTVPGM"
     gene            complement(6399..7850)
                     /locus_tag="PLY83_02420"
     CDS             complement(6399..7850)
                     /locus_tag="PLY83_02420"
                     /inference="COORDINATES: protein motif:HMM:NF025714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HOV28922.1"
                     /translation="MRKRTVLAVFLLVLASAGAWLLFPGSRRLSVTPPVPVPASPLPE
                     PPVSTLAVPLSVSLEELRVLARNRLPEEISGANAVRESLFEGRGTYLVRREGDPDVKV
                     ENDRLVLSIPVSFKARLNGTGTALGISLPVSLSAEGAATVELSMKPSVSPDWRVRTQP
                     KIAVKWSRAPAATVLGIRVTFQGAADEFLRARIEESLPRIDSALNESLNLRERAEEFW
                     REIQKPRPVSSSPDLWLTVRPLSIAIPPLRLGPEKVLLDARMEARLTLSTQKLNVPEP
                     VPLPPVSTSPGEGNRAGFTLELPVFLGYEALNRKIESELSNRTLAMGEGRKLTVRKIS
                     VSASGSRLVFALDVEALEGAGIFSSRRVGTVYVLGKPSWDPDRQVIRLTSVDFDEGTI
                     RGLVRTAAWIGRPLLLETLRQTAVFPLSEPAAQAKKTLGSFLERQEIGSGLTLRGSAR
                     QVALDSVAVTQEGLALLFRLAGETSLEWNPEKP"
     gene            7974..9236
                     /gene="eno"
                     /locus_tag="PLY83_02425"
     CDS             7974..9236
                     /gene="eno"
                     /locus_tag="PLY83_02425"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOV28923.1"
                     /translation="MSRICDVNAMEILDSRGNPTIRVFVELDSGARVSASVPSGASTG
                     ENEAVELRDGDKGRFGGKGVLKAVTNVNEILGPGLLGMDPTQQGQIDRMMIEMDGTPN
                     KAKLGANAILGVSMAVARAAAVDCGVPLYAYLGGCGARRLPVPMMNIINGGKHAENSV
                     DLQEFMIFPVGAPTFAEALRYGAETFHALKALLKKRGYSVGVGDEGGFAPDLKSNEEA
                     LELMVQAIESAGYEPGKQVAIALDPAASSFWEQGVYNLSKSGQGKKTSEEMVAMYKDW
                     VSRYPILSIEDGHAEDDWKGFSALTKEIGDRVQIVGDDIFVTNTNFIARGIEEKAANA
                     VLIKLNQIGTVTETIQAIELARKAGWGYVISHRSGETEDTFMADFAVAMGGGQIKTGS
                     VCRSERIAKYNRLLEIEAELDDAAVFGR"
     gene            9364..10098
                     /locus_tag="PLY83_02430"
     CDS             9364..10098
                     /locus_tag="PLY83_02430"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOV28924.1"
                     /translation="MPAEKARKGLKMKELEELTGESRATILFWISQGLLPPPVKTSAN
                     MSWYDPSYPKLIEVIRLFQRRMNQSIADIRAFVEQHGGPLALLSVREKIDDFLFGERS
                     PMWGSMGREELQKSAGIDSETLDRMLELGILRPECPGEFDEGDLNAASKVKSLVSLGI
                     PLEDLAFYGKAADSVARLEAEMVLREWRANPPEDLEKARQKAQRELSFARQYYFGRAF
                     RREMERIVRRVDQPVEAVAQKGGKRE"
     gene            10095..11375
                     /locus_tag="PLY83_02435"
     CDS             10095..11375
                     /locus_tag="PLY83_02435"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOV28925.1"
                     /translation="MKRLFPCGVFLGMLLAAPLPAMANSLLSSGGYLALVAEKNPELA
                     ASASRVESLYRLVEAVTGSTRPRLGLVASSGRILREGEGESTLSLSLGKRLDLSGLDA
                     LRIRQAELAAEIQEARHAEFAASLMREAETAYWNAAAGKSTVALYASLLAQRQEDLRI
                     ARLRFEQGTAARLDVFRAEVQVEKTRAAKTEAEAALRDALSLMARLAGGEAVEPSEIP
                     ARFETTLSADLEAAWKARPDARRAALEREAAAVATRIAALGMNPVLEASLASTLLSDS
                     STVVPPESDLLFSLRMEVPLYDGGQTRAEKQSRRILETAAEQDERALRVKVAMELAQA
                     SSRLAKAQALEMSRRKEVDLASEELRITRLRYRTGVGSQLELLDAQVREQAARTAYLD
                     ALREMAVARAQLSYALGLSTLPKAEAGSRPSGKE"
     gene            11383..12486
                     /locus_tag="PLY83_02440"
     CDS             11383..12486
                     /locus_tag="PLY83_02440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOV28926.1"
                     /translation="MRTLKELLRDRKTLAWMILAGIALGILVFRIAFPPRKAPAVSVS
                     AIRAEKGLPVTTLVAEKSSLDIRRSYYGLVRSVRTQEVTSFLREMVESVKVEVGDRVR
                     AGDVLAVLSGGEQAAAATADRASVEEAKRDVERMKTLYREGAVSRAELEQSVVRLKSL
                     EAVLSASSKRSARTVVRAKIDGVVTERHAEAGEIAEAGRPLLVIQDMGELEIEVLLPQ
                     ADRLYIVGGTDVEVPLEDRIVRGSVKRLDPAADPQTGLFRAIIAMPPDRALLPGATVE
                     ARILVKRSLGNVVLPSGIIQRGGDESFVYVVSGDRVRRRVVTLGEGADDLIEIRSGLK
                     PGETVVAEGSSALYDGARIWVQPAGAASGEVSP"
     gene            12483..15578
                     /locus_tag="PLY83_02445"
     CDS             12483..15578
                     /locus_tag="PLY83_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOV28927.1"
                     /translation="MNLIRFCIRRPVFTWVTVVLAAFLGLYGYFKLGVALYPKVDIPV
                     IVISAAYRGASPAEIESLVSKPLEDAVSEVDGVDKIESYSIEGVSFVVAEMKLDVNLS
                     EAVVDVSNRIKAARSKLPAEVDEPVVEKVDINAEPFMVAVFTTDMDGKKARDILEDKV
                     RRRLTRVEGIAQVALVGGEEREIHILLNPVAIRNFNLSPVLVASIVAANNLTNPAGYI
                     AQKDREITLRYKAEARDVKSLGEIQIPLGGGASVRLGDVARIEDGAKDLREFARFDGK
                     PAFQLQIKARPNANIVEVGAGVRKELEKILPTLPKGFRLDIPYDNSKFVSNSVRNVIR
                     DMITGTLLTALVLYLFLRRLSATAVVSIAMPTAVLATFIPMMIQGYTLNMMSTLGLAL
                     SMGVLVNNSILVLENITRFRDMGYDPIEAAERGTVEIALSVLATTGTNLGVFLPVAFL
                     PGVAGQFLVQYAMVIVYATLFSLWVSLTLTPMLAARMPQNPPTPLSCRLCGWWEWLYE
                     GLESLHHRLVVAAVSHPWKTLVLTGLLLAAVFALAPRLGSEFFPRVDEGVVSVSVELP
                     AESSLARTERTVRRIEEFVLKQPHVKNVVVSVGGTGARTGVNLGSVEAYLDEDPKRPS
                     TFEFADRIRPFLASLPDTTVSVQAVKTSFSRGKPLQIVVTGEETGVLSQIAGEVMKVI
                     RSVPGVVDVDLDWRVGRPELAMDPVRWRMGRLGSDAASVSSNIRGYITGLEAGKYREG
                     GLEYDILVKMEPQRTQSVFGTPDLPVMTSQGAVPVKEITRFSYAAGPTRIVRKDRVRS
                     VTVEADVVGRTVGEAFQEIRSGLRALPVPPGYAVKYEGDVEAMRETFTDMGVAFGLAV
                     GITFLMIAAILESWAFAFIIILTVPLSAIGVIPAMVATNTAVSLYALMGLVMLVGLVV
                     NNAIVIVDFAEGLRRNGTAAAEAITEACRVRFRSILMADLTSIFAMIPLALGMGEGGA
                     LRAPMAIVAIGGLFAGGGLALFVIPPVYRLFWERRERRALPARRRLRDRLAGWFGRFR
                     G"
     gene            complement(15568..16089)
                     /locus_tag="PLY83_02450"
     CDS             complement(15568..16089)
                     /locus_tag="PLY83_02450"
                     /inference="COORDINATES: protein motif:HMM:NF015060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HOV28928.1"
                     /translation="MIASTQLQKGGISLNRPDFLRLAACILVCQAAGILGSFFTASSL
                     QSWYTGLVKPAFTPPGWVFGPVWTVLYTLMGIAAWLVWRRGSLADPNIRNAVFLFLIQ
                     LSLNTLWSALFFGLRSPLLALVDILALAAAILATLAAFKPVSPLAAWLMVPYLAWVSF
                     AAILNASIWKLNP"
     gene            16129..16839
                     /locus_tag="PLY83_02455"
     CDS             16129..16839
                     /locus_tag="PLY83_02455"
                     /inference="COORDINATES: protein motif:HMM:NF016382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HOV28929.1"
                     /translation="MKIEWNDSEEWGRFCKGALSPAEAAALQVRLSGRVSLEDARSGL
                     PSTVAGVDVSIRRGPLGDVAVACIALMEWESWELLGVFRAEEPVRFPYIPGLLSFREL
                     PVILRAAEGLPEPPGLFMVDGAGIAHPRFFGLACHLGVVTGCPSVGVAKSRLVGTHDP
                     LPGERGSRVPLLVEGKEVGTVLRTRRGVRPLYVSPGHRVSLGTSGDLVLAACTRFRLP
                     EPVRAAHKLAGDQTLLYS"
     gene            16927..17826
                     /locus_tag="PLY83_02460"
     CDS             16927..17826
                     /locus_tag="PLY83_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HOV28930.1"
                     /translation="MLITIADPFDPSLPGKLGQFGEVATGLDRLAETDVLLVRSKTKV
                     TREILEKAPKLKLVIRGGVGIDTIDTKAAEEKGVRVRNTPRASSITVAELAFALMLAV
                     PTRLIEAHISMCQGKWEKNELKRTELFGKTLTLVGLGNIAAEVAKRAQAFGMTVCAYD
                     PHVPAQRAEELGVTLLGDLKEALSRADYVSLHVPLVESTRNLINRETLSVMKKGAVLV
                     NTARAGVIVAEDVLEALESGQLAYYCADVWPTDPPSPDYPILKSNRVFMVPHLGANSK
                     ENLLRIGEEVCSIIREAQKEGVL"
     gene            17828..18913
                     /gene="serC"
                     /locus_tag="PLY83_02465"
     CDS             17828..18913
                     /gene="serC"
                     /locus_tag="PLY83_02465"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HOV28931.1"
                     /translation="MPRKLNFHAGPSTLPVEVLEELQKNMVDFEGTGLSLIEESHRSA
                     TYEAVHNRAIELFRELFSVPENYDILFLGGGATLQFAMIPMNFLPQGKTAEYVATGTW
                     SKKAIDDAKKLGSVRIIFDGKESGYTTLPDPASVKVDPESAYLHITANETIGGLEWKD
                     WPETGGVPLVCDMSSDILSRPVPVEKFALIYAGAQKNLGPAGTTVVILRKDMLDRIPD
                     TLPAYLSYKNHAANNSLYNTPPVFAIWTIKLVLEHMKKNGGLAAVAERNLKKAGLVYG
                     VIDSSGGFYRSPVDARYRSNMNIVFRLPSEELEKKFIDEAKAAGMIGLKGHRSVGGCR
                     ASLYNAMTLEGAEALAAFMKEFAAKNG"
     gene            18924..20195
                     /locus_tag="PLY83_02470"
     CDS             18924..20195
                     /locus_tag="PLY83_02470"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HOV28932.1"
                     /translation="MGDVRARLEKVGIGLPEILLPKAGTDLSKWSVVACDQFSSERHY
                     WEKVREIVGEAPSTLRLILPECYLEDGDTEARVEAINAEMKRVLETVLEPLEPGFVLI
                     DRRTAHAPSRKGLVLAVDLEAYSFEAGAESLIRPTEGTILKRLPPRMAIRRGATLDLP
                     HILLLLDDPQRTVIEPLFSVRENFKKLYDFELMQGGGSIAGWHLPAEALGDTVTALEK
                     LASGGFLFAVGDGNHSLAAAKSLWEERKAAGAGPDDPARWALVEVENIHDEGLHFHPI
                     HRVLFGVSVDAVLDEMKKALGGRMEAGTANPAPKTEAGHVIGVVSEKGEGRFVFKPKE
                     ERMTVEFFQEFLDEYLAKHPEVTIDYIHGEEAVREMGSKPGNAGFTMPPLDKSVFFRR
                     MVEVGPYPRKTFSIGEATEKRYYLESRKLVR"
     gene            20427..21182
                     /locus_tag="PLY83_02475"
     CDS             20427..21182
                     /locus_tag="PLY83_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV28933.1"
                     /translation="MSTLSLDYPGEGAESVSTSFNFQWTLTPDVDVTSPDLVSYDLYL
                     SKTLSGLDAGVLSQDLKFDGYEGTFPSGTLDEDSLYFWKVVAHTPSGDVGATSFFMTQ
                     GEGASLVLKSPSNGQTGVAPQNVEFKWEFDSGDNSLTAEDVTFDLYLSDKYETIFDGA
                     PKRSGIKALDPDVVDGVFSVTINQSVGYGKHYWTVVAHVDGDGDFTTSPHSFTTLSEA
                     SDPNLSGGCSAAPLGGLAAMVLLAGLVLVKRGR"
     gene            complement(21272..23632)
                     /locus_tag="PLY83_02480"
     CDS             complement(21272..23632)
                     /locus_tag="PLY83_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HOV28934.1"
                     /translation="MNDRIARLRKESFETHPSISIERALLVTEFYQENAGKYSTPVMR
                     ALNFKNLCEKKTIYIGPDELIVGERGPAPKAVSTFPELNCHSREDLEILNSRKMTSYS
                     VSPEDIDLYAEKVIPYWRGRSMRDKAFAQIPAKWKALYEAGLFTEFMEQRAPGHTALD
                     GTIYRKGMLDFKREIAESKAKLDYLNDPHATEKFEELTAMDISCDAAILFAERHADLA
                     EKMAAEEKDPKRKAELLKIASVCRWVPAHAPRNFWEALQMYWFVHLGTITELNGWDAM
                     SPGHLDQHLAPFYEKDLAEGTLDREGARELLSCFWIKVNNTPAPPKVGVTAAESGTYN
                     DFTQINLGGLKADGTDGSSEVSTLILEVLDELQLLQPQVSVQISAKTPDAFLKFAAKV
                     IRRGSGYPSVFNADMVIAEQIRVGKTPEDAREGGTSGCVETGAFGKEAYILHGYLNVP
                     KILELTLNNGVDPLTGKKVGLETGDPEGFTTFDELYSAFEKQLRYVVETKIETDNYLQ
                     RMFATYAPAPFLSVVINDCIKNGKDYYDGGARYNSDYIQCCGIGTITDSLAVLKKHVF
                     EDNTITMRTLLDALKKNWEGEEPLRQFVWNKTPFFGNDDDYADSIMQRVYDSLFRTIE
                     GQHSPKGPTYHLNMLSTTCHVYFGKKLGATPNGRFAFLPESDGTSPSHGADRHGPTAV
                     VKSLAKMDQVKSGGTLLNQRFLPSVLAGESGIDALCQLIRTYFRLGGHHMQFNVVDSD
                     TLRDAQKHPENYRDLLVRVAGYSDYFVDLDQYHQEEIIARTAQESF"
     gene            complement(23638..24543)
                     /locus_tag="PLY83_02485"
     CDS             complement(23638..24543)
                     /locus_tag="PLY83_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOV28935.1"
                     /translation="MKSGIVFDFKKYSIHDGPGIRTTVFLKGCPLSCWWCHNPESISP
                     QPAVLYRPERCIGCGACLKACPNGAINATPEGFETDFSRCVGCGACADACPSTARERV
                     GRLMTSEEVLTVIRKDIPFYDESGGGATFSGGEPLLQPEFLWELLEGCRKSQIHTAVD
                     TTGFAQTAVLLETARLTDLFLYDIKHMDPGKHKLYTGVDNTLILENLKALTDSGARVA
                     IRIPLIPGINDDEENLRQTGRFVAALKGVVSVHLLPYHAAGRNKYAKWKIAYRLPETL
                     PPSAEAQEAAAENLKSFGLNVLIGG"
     gene            complement(24618..25787)
                     /locus_tag="PLY83_02490"
     CDS             complement(24618..25787)
                     /locus_tag="PLY83_02490"
                     /inference="COORDINATES: protein motif:HMM:NF017466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid recognition domain-containing
                     protein"
                     /protein_id="HOV28936.1"
                     /translation="MLRSCAQELAESTSEIAGYSVLLTDENGIVIGSSDPSRLGSLHS
                     PSLGVIRTRKPEITTPEEAHRLPEGVRPGITLPISLAGRVVGSIAIAGPPEEVSRYGR
                     LVQKQAELLLRENALLRSSMLREQALQELILEIAAFDPLKADETLLVMRGRELGYDLR
                     PPRAVLVAEILHPEKAPLNLRLEAQALARRFFSHPQDIVSRLGDDKIVVLAFLGLAVR
                     EIQPLEEVRNTARLLALSLEEQGLSARIGIGEIAASAASLGEAFRSAGEAARLSLKKC
                     GEEKVFYIGDLRLESLLSAVPKDRAAAFCESVLKDLPGASDAEELIQTFLAWCDSPFE
                     PTRVAQTLSIHRNTLAYRLQKIGRLTGLDLREIRGILMLRSALELRKTADFRRKA"
     gene            25984..27270
                     /locus_tag="PLY83_02495"
     CDS             25984..27270
                     /locus_tag="PLY83_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HOV28937.1"
                     /translation="MRLELHEVHVSDIQWGERTEVVDHVLRVNREEAAAALAGEDRFA
                     SVRLDLARPGESVRIIPVKDVVEPRVKLKGGRGYFPGFFAPPETAGEGVTLVLKGAAV
                     VTCGPIVAFQEGFIDMSGTGADYTPFSKTNNLVLTVEPVEGLGKHAYEEALRLAGLKL
                     ALYLAEACREAKPDEIRVFEKGSITEENEKYPNLPRILYICMAITQGLLHDTFLYSAD
                     MKSVLPTLLHPNEVLDGALVSGNCVSACDKNTTYHHVNNPVVEALYRRHGKDLNFLGV
                     VPTVEDVRLAGKERASSMNAALAKNLGAHGVIVTEEGYGNPDTDLCLNAKKCENLGIR
                     TVVISDEAAGTDGASQSLADATPEMSAFVSSGNVNEMIEVPPMKTVLGHPEAIANLSG
                     GAAESLRPDGSMFVELQAVIGSTCELGFNRIGCEWI"
     gene            27291..28604
                     /locus_tag="PLY83_02500"
     CDS             27291..28604
                     /locus_tag="PLY83_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:28335..28337,aa:Sec)
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HOV28938.1"
                     /translation="MTIRVVHYINQFYAGLGGEEAANALPESRKGAVGPGVQLQTLFG
                     EKAEIVGTVICGDGTYGERTEEARADCIRLIEAFQPDLLVAGPAFNAGRYGFACGDIC
                     SAAGKMLGIPTVTALYPENPGVELFAKNTVIVQAADSARGMKAALEGLARVGLKLASG
                     EPLGPARLEGTISRGIRKNFFFEKNGAERAVEMLLDKIAGRPFRTEYEMPVFKRIPPA
                     PPIKDLKTATIALVCSGGIVPKGNPDHIRVSSAETYGRYNISELNDLTPETYESIHGG
                     YDRVWANQDPDVVLPVDVMRELEREGVIGKLYDWIYTTTGTGTAVAHAERFGREIGAQ
                     LKEAGVDGVILTSTUGTCTRCGASMTREIEKAAGIPVVQIATIVPIMLTVGSNRIVPG
                     VAIPHPVGDPAKGPEADKKVRRELLLKALEAMTTPIEQQTLFERK"
     gene            28601..30142
                     /locus_tag="PLY83_02505"
     CDS             28601..30142
                     /locus_tag="PLY83_02505"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOV28939.1"
                     /translation="MSLSKPMVRRSETLIPVSKNPPAKVKDKGLIRLARSGAAAYDAS
                     ADRILWLPWGVSLLDRVRRGLSSRFRSAGAQPVLCGQAPSGTLPVAVRVLKTADQLPL
                     TLMEEDRTLILLEGFDPEGAHPGWPVYAERLLSFLQERGMELSVFQEALPFGERTLVA
                     CSCEGEARGALEARVCPACGWTASVDALLPPVEEADEEERPLEAVLTPGATTIAELCR
                     QLGCPPDRTIKTMFYAAEKDGKTFVAAALVRGDRAVCPTKLSRALGGAAVRRAESADL
                     KQAGCDVAGFLGPVGLPETIRVIADASVAGTKNRVIGANRPETHFTGACWGRDYSAPV
                     ADISALEPGGACPACGRPVEKGWIRPLAEFAKGHPALAAFPSLGYLDENRKRRLPGTW
                     TGRILLEAIALASAAEGDAPFRLGLSPADLLVIPLGAGGREASKAAEAVADELEHRGW
                     TVLLDDRPLPDEARLAEASLAGCPVQVLVADGLEAEVTGVGGGPGKRVPLEGIGGFLE
                     PLRGA"
BASE COUNT         5789 a         8832 c        10040 g         5600 t
ORIGIN      
        1 cttgcttttt ttctcccggt tctttataat cactccaatt tcgtgggggg tgtaggaatg
       61 gttcgtccgg ttgtccagtc cagctgttgt tgtcggtgga ttttttcctg atccaccgcg
      121 gcccgcaggg tcgcggcggc accattcgct gcggccgggg ctgcaagggc aaccggttcc
      181 tcccatagga gaactcccgt atcgaaacga tgatcgggct ggccgcgagg gcagcccgat
      241 tttttgtttt cgagaggagg agaagcagat gggagcatgg agaacgcttg ttgaggattt
      301 caaggcggtc aaggaaaggg atccggcgat cccttccggg atccgcggtt tcttcgaggt
      361 cgtcctttgc acccccggtt tccaggcggt ggcggctcac cggctgatcc attttctcca
      421 cgtccggatg cggattcccg ttcttccaag gttccttggc cttcttgtcc gctggtggac
      481 gggaaccgag atccacccgg gggcacgcct cggaaagggg gttttcatcg accacgcggc
      541 gggtgtggtg atcggggaat cggcgatcgt gggggacaac gtgacgctgt ttcagggagt
      601 gaccctcggg gctaccggca acgagaaaac ctggaaaagg catccgaccc tggagtcggg
      661 ggtctttgtg ggaagcgggg ctcgaattct gggcccggtc acggtaggga agaatgcccg
      721 aatcggagcc ggaagcgttg ttctcaagga tgtgcctccc gaaacgacgg ttgtcggcgt
      781 tcccgctcgg gtcgtcaaat ccgggggacg gagggtccct tcccctctgg agcgggctga
      841 gagcagaatc gaggcccttg agggaatggt ccgggatctc caggaagaag tccaaagact
      901 tcgcgaggct tcggcgtttt atgggccgtc gaagtttaaa gctgggaaag gggtggcctg
      961 agatgtcctt tcgggtggat tcgctggtct gtttttctct tgtcgggaac actccgatgg
     1021 cccggatcga ggccggcggg ggaacggaaa tctggatcaa gatggaaggg aacaaccccg
     1081 ggggatcgat caaggaccgg gcggcgtggg gcatgctgag gaaggcggaa gaggaaggcc
     1141 tcctgcaaga aggcgcggta atcgtcgaac cgacttccgg aaatacgggg atcgccctgg
     1201 cgatgttggg aaggtcgttg ggcttacggg tgatcctgac gatgcccgaa tcgatgtccg
     1261 cggaaaggag agcggtgctc gcagcctacg gtgcggaact gatcctgacc cccgccgcgg
     1321 aaggaatgga agggtcggtc cggaaagccg aagaggtgac gcgggagttg aacggagcct
     1381 tcatgccggg tcaattcgac aaccccggca acccgtggtc tcaccaggtc acgacggggc
     1441 cggagatcct taaagccctg aaggggaaag cccctttcgc cttcgtggcg ggagtcggaa
     1501 cgggagggac cctcaccggg gtgggcagga gcctgaagtc ggcgttcccg gatgtccgga
     1561 tcatcgcggt ggaacccgcg aaaagccccc ttttgggcgg aggaaggagc ggggcccacg
     1621 gaatccaggg gatcggggca aactttatcc cgaaaaatct ggaccgctcc ctcgtggacc
     1681 aggtcgagcc cgtgacagac gaggcggctt ttgatatgac gcggtggctg gcgagggaaa
     1741 agggactttt ctgcgggatc tccaccggtg ccaacgtggc ggctgccttt tcggccgcgg
     1801 cgaaaatgcc ctcacccggc atcgtggtaa cggtatcacc ggaccgcggg gaccggtatc
     1861 tcagcacggg cgttttcgga cggttctgag gctatttcct ccacgttccc gggacgaaca
     1921 gcatgaggac ggtgaagagt tccaggcggc ccaggagcat gcagagcgaa aggacccact
     1981 tccctgccgc cgggatcgcg gcataattgt ccatcggccc cacgataccc aggcccgggc
     2041 cgatgttgcc gagggttgcc gccaccgagg agatgcccga aacgaggtcc agccccagtg
     2101 cagtcatgac aagggaggcc ccgacaaaaa gggccatgta gacgatgaag aaaaccgtga
     2161 cggagttgat cgcctcccgc tcgacgacct tgcctccgac cctcggaacg atcacggccc
     2221 tcggatggag tagccgccgg atttcagatc cggcctgcct ggcaagcagg aggatccgga
     2281 tgttcttcat gcctccgccg gtggagcccg cgcagccgcc cacgaacatc agaaccagca
     2341 gcaaaagctg ggtgtaagcc ggccacttct cgaaatccgc agtgacgaaa ccggtcgtcg
     2401 tcaggatgct cacgacctgg aaggccgaaa aacgcagggc ctccccgaga gagtcatagg
     2461 cgttccctga gaggaggatg accatcaccg tcgctgttcc gatcagggta acccaggagt
     2521 agaatcggaa ctcctcgtcc ttccagaaga ccgaaaggcg ccctcggagg gcgaagtagt
     2581 gaagggtgaa gttcgctccc gccaggaaca tgaagacggt gatgatccag tcgacgaaag
     2641 ggcttcggta gaagccgaca ctcaggttct ttggagaaaa gcctcccgtg gccatggttc
     2701 cgaaggtgtg ggtgagagcg tcgaacaggt tcatcccggc tagcacgagg aggaccgttt
     2761 cggtcagaga aagaatgacg tagacccccc aaagcaggag ggctgtctgc tggatccggg
     2821 gcgtcagctt ctcgggcacc ggcccgggga cctccgcctt gaaaagctgc attccgccga
     2881 cgccgaggaa gggaaggatg gccaggctga gaacgataat ccccattccg ccgagccagt
     2941 gggtcaggct tcgccagaat agaatccccc gggggttggc ctcgatgttc gtcagaaccg
     3001 aggccccggt ggtggtgaat cccgacatgg cctcgaaaaa ggcgtccgtg tacgtggaaa
     3061 ccgacccctg gaggtagaag ggcagggcgc cgatggcgga gcacaggatc caggacaggc
     3121 tcacgacggc aaaggcctcc cttggcccca gatccttggc gctcgcattt ctgcccagga
     3181 agaacaggat cccggaaatc gacaggccga tcaaaaggga agcacccagg gcggccaggt
     3241 ccgacgagcg gtcccaggct gcccacgcca tgccccagag gaggaagagg gagacgacgg
     3301 tcgtcaccag gctcagaact tttgcaacga tggcggggcg cattcctcag tcggctccca
     3361 gcgtgtccag ggccttgtgg agatggtccc tggaagagaa gaacaacagg tgatcgtttt
     3421 ccttcaggac ggtgtcaccc tttgggacga agacctccgc tcccctttcc acgagggcca
     3481 ggatcacgcc cttggggagt tccagatcca tcaccttttt cccggctgcc ggagaatccg
     3541 gggtgagcgt gatctccccc atctccgcgt cgatctggtc gatgaccgag accgccccca
     3601 tcccccgggg atagcggatc cggtggagaa tcgtcgaggc cagcgcttcg ttcgggttca
     3661 ccagcgcgtc ggcatgaaag gcctcctgga gttccaggta gctgcttttc cggatcacgg
     3721 cgatcgtctt tcgggcaccc aggtttctgg ccacggcaga aaggaccagg ttcatctcgt
     3781 cgttggccgt ggtgcacacg aacccgtccg tttcctcgat cccctcgtag cggaggaggt
     3841 cgctgtcggt ggcgtcgccg tgcaggaccg tggttttctc gagttcccgg gccaggatct
     3901 cgcatttctc caggtcgcgg tcgatcaggc ggatgtcgac cttctggaag ttttcttcaa
     3961 ggcgtttcgc cacctggaag ccgattttcc ctcctcccac gatgatgacc cggcggagcg
     4021 gccgggccga cttgcccgtg aagagtcccg ccactcggtc ccgttcttcc ctgagggtgg
     4081 cgacatagca gagatcccct tccaggagga cgctttctcc cgaggggaca aagccgcttc
     4141 ttccacgccg gacgtagacg atgatggttc ggagtttctg gtactcccgc gtcaggtctt
     4201 tcagagggcg ccccagcagg ggggatcctc cggaaagggt gaagccgtag atgccggccc
     4261 gttcctgcat cagggtcgcg gcgtgaaagg aagctcctac ctggagaagt tcctggattt
     4321 cgcgggcaac gctccgttcc ggggagatga gaaggtcgac gttcaggtcc cgggcccagg
     4381 actgggtgtc ggtgaactcg atgctccgga cccgggccat cgtctgggga accccgcttc
     4441 tcttggcgat ccagcaggcg aggatgttca cctcgtcgcg gtccgtggcc ccgatcaaga
     4501 ggtccactcc tccatcttcc cccaggccgg cttcctccag gacctgcggt cgcgccccgt
     4561 ttccccggac gacccgggca tccagctcct cgtcgatttg ctgggcccga tcctcgtccc
     4621 gctcgacgat ggtcaggtca tgaccgtccc gaatgagctc ccttgcgaca ttctggccga
     4681 cttcaccggc cccgaccagg atgattctca tcgtctcaat cctccatgta gtccgccgga
     4741 tccacgatcc cgaggggagg ttctccggcg agaaaccgac agacgttgtc cacggccgcc
     4801 tgcagggcga ccggaaagat gccgtttacc aggttcgagt tgtgggggga gccgaggaag
     4861 ttctcgaggc ggaagaaggg gaaatggacc cggaattccc cgtgggttgc gggttcgatc
     4921 caccaggtgt caactcctgc ccggaaggag gggttggcct tcaggtgctc gtacagggct
     4981 tcctcctgga tcagcgcccc tcgggccacg ttcacgagga tggcgtcttt cctcatccat
     5041 tccagctgtt tctttccgat catcccccgc gtcctgcgcg tgaggggaag gctcagcacg
     5101 acgacatggg cgtcccgcac ggcttcctcc agctggtcca atgttccgat ccagtcgacg
     5161 ggttccaggg tttgtcccgt cgtgttgacc gcgcggatct ccatgccgag actccggaaa
     5221 agccgggcca cggctttccc gatcccgccg aagcccacga tcgcggccga ggccttgcgg
     5281 aaccagagac tttctctgct gttgtcgaat tctccccgcg ccagttttgc gtgaaggggc
     5341 aaaaggttct tccccagggc cagggccatg gccagggcat gctcggcaag ggggtcagcc
     5401 caggcccccg cgttgctgca aaggatcgcc tgtttgggca tgaggggaaa cggcagggcg
     5461 tcaacaccgg ccaggaggga ttgaaagagc ttgaggttct tgagaaacag cagctccggc
     5521 gaggggattt cccggccaag gatgaagctc agcaaaacat ctgcggattc gagggcggac
     5581 ctgcggcttt cgatgtcgcc gaggtccttg agaaaaacca gccgggacga ttctcccagt
     5641 tcccggtaaa gcagcgaccg ggcttcgcta tcggcgggga aactgacgac gatcaccggg
     5701 ttccgttcca aggcggttcc tccctgaagg gcttgcgcga tttcccctgg gcggggacaa
     5761 tgccaatcat agaccaattt caccttccag agaaagggga cgggagcatg aagggccgtt
     5821 tttgcgtcaa ggcagccggc aggatccttt tcaccgcggc gttggccatc gttctatcgg
     5881 ccgtcgctgc aagcgccgcc gagttttcgg ccgacatggt catgaagggc ccagacggtc
     5941 aggcgatgtc cggaaaggtt ttcgtcaagg gacaaaaaat ccggcaggag attcagcagg
     6001 atggggaacg ggtgatcacc atcctccgga tggacaaaaa gctttcctgg gttctcatgg
     6061 tcgacgagaa gatctacatg gagatgaaga tgcagaaaag cgcggaagat ccttccctcg
     6121 tggaaaagac cgatcctgcc aagatcaggc gcctggggaa ggaaacggtc aacgggtttg
     6181 tctgcgagaa gatccaggtc accgagaagg acgcggtgat cacccagtgg atttccgaag
     6241 atctcggctg gccgatcaag ggcgaggcca agacgccgga gggcgtttcc tctcacgagt
     6301 acaaaaatat ccggaaagga aatgtcccgg acgacctttt cgaggttccc aagggattcc
     6361 agaagatgac cgtgccaggg atgtgaggaa gttttggatc acggcttttc gggattccac
     6421 tccagcgagg tctcgcccgc gaggcgaaac agaagggcca gtccttcctg ggtgacggca
     6481 accgaatcca gggcgacctg tcgggccgag ccccgaaggg ttaggccgct cccgatttcc
     6541 tgtctctcaa gaaaactccc cagggttttt ttcgcctggg cagctggttc cgaaagggga
     6601 aaaacagccg tctgccggag ggtttcaagc agcaggggcc gtccgatcca ggccgcggtg
     6661 cgcaccagcc cgcggatcgt cccttcgtcg aaatcaacgg aagtcagccg gatgacctgc
     6721 cggtctggat cccacgacgg tttcccgagg acgtagaccg taccgacgcg gcggctggag
     6781 aaaattccgg caccttcaag ggcctccacg tccagggcga aaaccagccg gctgccgctg
     6841 gcggaaaccg agatcttgcg gacggtgagc tttctcccct cccccatcgc caacgttcgg
     6901 ttcgacagtt cgctttcgat cttccggttg agggcttcgt agccgagaaa gaccgggagt
     6961 tcgagggtaa acccggcccg gtttccttcc cccggggagg tcgaaaccgg gggaagcgga
     7021 accggttccg ggacgttgag cttttgcgtg gagagggtca atcgggcttc catccgggcg
     7081 tccagaagga ctttctccgg tccgagcctg aggggcggga tggcgatcga aagagggcgg
     7141 acggtcagcc acaggtccgg cgaggacgat acgggcctgg gcttttggat ctcgcgccag
     7201 aactcttccg cacgttcccg gaggttcagg gactcgttca gtgccgaatc gatcctgggg
     7261 agggactcct cgattcgggc ccggaggaat tcgtccgccg cgccctggaa ggtcacccgg
     7321 atcccaagga ccgtcgcggc cggtgcccgg ctccacttca ccgcgatctt cggctgcgtc
     7381 ctcaccctcc agtcggggga aaccgagggc ttcatcgaga gctcgacggt ggccgcccct
     7441 tccgcggaaa gagaaacggg cagggaaatt cccagggcgg ttcccgttcc gttcagcctc
     7501 gctttgaagc tgacggggat tgacagaacc aggcggtcgt tctcgacttt cacgtctggg
     7561 tcgccttccc tccggacgag gtaggtcccg cgcccttcga agaggctttc cctcacggcg
     7621 tttgcgccgc tgatttcctc cggcagccgg ttcctggcca aaacccgcag ttcctcaaga
     7681 gaaacgctca ggggaacggc aagggtggaa accgggggtt cgggcagggg tgatgcagga
     7741 accggaacgg gtggggtcac ggaaagcctc cggctgccgg gaaacagcag ccaggccccg
     7801 gccgaggcca ggacgagcag aaagaccgct agaaccgttc tctttcgcat gggcattccc
     7861 cccggcccat tttatcctga agagcagggg aaccggaaaa gcgcgctaca atgggaagga
     7921 aaagaccgcc cggcttgatc gggtgaaaac aaaaaggaaa gggtggtttc tggatgagcc
     7981 ggatttgcga tgtgaacgcg atggaaatcc tggattcgag gggcaatccc acgatccggg
     8041 tattcgttga gctggatagc ggagcaaggg tgagcgcctc cgtcccctcc ggggcttcga
     8101 cgggcgaaaa cgaggcggtg gagctcaggg atggagacaa ggggcgcttt gggggcaagg
     8161 gcgtgctgaa ggcggttacg aatgtcaatg aaatcctcgg cccgggcctg cttggaatgg
     8221 accccaccca gcaggggcag atcgaccgga tgatgatcga gatggacgga acacccaaca
     8281 aggccaagct tggggccaac gcgatcctcg gggtttccat ggccgtcgcc cgggccgccg
     8341 cggtcgattg cggggttcct ctctacgcct atctcggagg ttgcggggca aggagacttc
     8401 cggtcccgat gatgaacatc atcaacggag gcaagcacgc ggaaaacagc gtcgatctcc
     8461 aggagttcat gattttcccg gtcggggccc ccacgtttgc ggaggcgctc cgttacggag
     8521 ccgagacctt ccatgccctg aaggcccttt tgaagaaacg gggatattcc gtcggcgtgg
     8581 gcgacgaagg agggttcgcc ccggacctga aaagcaacga agaagcgctg gaactgatgg
     8641 tccaggccat cgaatcggct gggtatgaac ccgggaaaca ggtggccatc gccctggacc
     8701 ccgcggcgag ctccttctgg gagcaggggg tttacaacct gagcaagtcc gggcagggaa
     8761 agaagacctc ggaagagatg gtggccatgt acaaggattg ggtgagcagg taccccatcc
     8821 tttccattga ggacggccat gccgaggacg actggaaggg cttctcggcc ctgacgaagg
     8881 aaatcggcga cagggtccag atcgtcggtg atgatatttt tgtaacgaac acgaacttca
     8941 ttgccagggg catcgaggaa aaggcggcca acgccgtcct gatcaagctc aaccagatcg
     9001 ggaccgttac cgaaaccatt caggcaatcg aactggcccg gaaggccggc tgggggtacg
     9061 ttatctccca tcgctccggg gagacggagg acacctttat ggccgacttc gccgtggcca
     9121 tgggtggagg gcagattaag acggggtccg tatgccgcag cgagcggatc gcgaaataca
     9181 accgcctgct ggagatcgag gctgaactgg acgacgcggc ggtcttcggg cggtaggaac
     9241 gggcaggaag gcccgcgggg aaggatttcc cgcgggcctt ttttcttttt gttgtaaagt
     9301 gaaaagtgtt gctttatgct ttatggactt ggccaatcgg ccgcctttca gggagggacg
     9361 gcgatgcccg ctgaaaaagc ccgcaagggg ctgaaaatga aggaactgga agagttgacc
     9421 ggagagagcc gggcgacgat tctcttctgg atttcgcagg ggctgctgcc gccccctgtc
     9481 aagacaagcg cgaacatgtc atggtatgac ccgagctacc cgaagctgat cgaggtcatt
     9541 cgcctgttcc aaaggcgcat gaaccagtcc atcgcggaca tccgggcgtt tgtagaacaa
     9601 cacggcgggc ctttggccct tctgtctgtc cgagaaaaga tcgacgattt cctgttcggg
     9661 gagagaagcc cgatgtgggg cagcatgggc cgggaagagt tgcaaaagtc cgcggggatc
     9721 gactcggaaa ccctggaccg gatgctggag ctgggcatcc tccgcccgga atgccccggg
     9781 gaattcgacg aaggagacct gaatgccgcc tcgaaggtga aatccctggt ttctctcggc
     9841 attcccctgg aggaccttgc cttttacgga aaggcggctg actcggtggc tcgcctggag
     9901 gcggaaatgg tcctgaggga atggcgggca aatcccccgg aggatctcga aaaagccagg
     9961 cagaaggcgc agagggagtt gagcttcgcg agacagtatt atttcggccg ggccttccgg
    10021 agagagatgg agaggatcgt tcgcagagtt gatcagcctg tcgaggcggt agcgcagaaa
    10081 gggggaaaga gagaatgaag cggctttttc cttgcggggt tttcctggga atgctgttgg
    10141 ccgctccgct tccagccatg gcgaattcgc tgctcagtag cggagggtac ctggccctgg
    10201 tcgcggaaaa gaatcccgag ctggcggcat ccgcctcgcg ggtcgagtcc ctgtacaggc
    10261 tcgtggaagc ggtgacgggt tccacccggc cccgcctggg gttggtggcg agttccggcc
    10321 ggattctgag ggagggggag ggagagagca ccctttcatt aagcctgggg aaacggctgg
    10381 acctttcggg ccttgacgcc ctccggatcc gccaggcgga actggcagcg gagatccagg
    10441 aagcgcggca tgcggagttt gccgcatccc tgatgaggga ggcggaaacc gcgtactgga
    10501 acgccgcggc cgggaaaagc acggtggctc tctatgcctc gcttttggct cagcggcagg
    10561 aggacctccg gattgcccgg cttcggttcg aacaggggac cgctgcgaga ctggatgtgt
    10621 tccgagcgga agtgcaggtc gaaaagacgc gggcggccaa aaccgaagcc gaagcggccc
    10681 tgcgggacgc cctttccttg atggcccgcc tggcgggcgg agaggcagtc gagccctccg
    10741 agatccctgc ccggttcgaa acgacccttt cagcggacct cgaggcagcc tggaaggcac
    10801 ggcccgatgc gcgaagagcg gctctcgaaa gggaagcggc tgccgtcgca acccggatcg
    10861 cagccctggg catgaatccc gtcctggaag cctcccttgc ctcgactctc ctgtccgact
    10921 caagcaccgt cgtgcctccg gagtccgacc tcctgttttc actcaggatg gaggtgcctc
    10981 tttacgacgg cgggcagacc cgggccgaaa aacaatccag gagaatcctg gaaacggcgg
    11041 ccgagcaaga cgaaagggct ttgcgcgtga aggtcgccat ggagctggcg caggcctcaa
    11101 gccggcttgc gaaggcgcag gcgctggaaa tgagccgaag gaaggaagtg gacctggctt
    11161 ccgaggagct ccggatcaca cgcctcaggt accggaccgg tgtgggctcc caactggagc
    11221 ttctcgacgc gcaggttcgc gagcaggctg caagaaccgc ctacctggac gcccttcggg
    11281 aaatggccgt ggcccgggcc cagctttcct atgcccttgg cctcagtacg cttccgaagg
    11341 cagaagctgg aagtcgcccg tcgggaaagg aatagtgaaa aaatgagaac gctgaaggaa
    11401 ttgctgaggg atcgaaaaac cctggcgtgg atgatcctcg cggggattgc cctggggatc
    11461 ctcgttttcc ggatcgcctt ccctccccga aaggcacccg ccgtttcggt ctccgcgatc
    11521 cgggcggaaa aggggcttcc ggtgaccacc ctggttgccg aaaagagcag cctcgacatc
    11581 cggcgaagct actacgggct ggtccgttcc gtcaggaccc aggaagtgac gtctttcctg
    11641 agggaaatgg tggaatcggt gaaagtcgag gtgggggacc gcgtccgcgc cggggatgtc
    11701 ctggccgtgc tctccggggg tgagcaggca gccgcggcga cagccgaccg ggcctccgtc
    11761 gaggaggcca aaagggacgt cgagcgaatg aaaacccttt acagggaagg tgcggtttca
    11821 agggcggagc tggagcagtc cgtggtgagg ctgaaaagcc tggaggccgt cctgtcggcc
    11881 tccagcaagc gctcggcccg gaccgtggtc agggcaaaaa tcgacggcgt tgtgacggaa
    11941 cggcatgccg aagcgggaga aatcgccgag gcggggcggc cccttctcgt catccaggat
    12001 atgggggaac tcgagatcga ggtgctcctt ccccaggcgg accgattgta catcgtcggc
    12061 gggacggacg tggaggtccc ccttgaggac cggattgtcc gaggcagcgt gaaacgcctg
    12121 gaccctgcag cagaccccca gaccgggctg tttcgggcga tcattgccat gcctcccgac
    12181 cgggcgcttc taccgggtgc caccgtggaa gccaggatcc tggtgaagcg gagcctgggg
    12241 aacgtggtcc tgccctcggg gatcatccag agaggggggg acgagagttt cgtctacgtg
    12301 gtctcggggg accgtgtccg gcgccgggtg gtcaccctgg gggagggagc agacgacctg
    12361 atcgagatcc gatcggggtt gaagccgggt gaaaccgtcg tcgccgaggg ctcgtcggct
    12421 ctctatgacg gtgcccggat ctgggttcag ccggctggag ccgcttcggg cgaggtctcc
    12481 ccatgaacct gatccggttc tgcatccgcc gcccggtgtt cacctgggtg acggtcgtcc
    12541 ttgcggcctt tctggggctg tatggctatt tcaagctcgg tgtggcgctc tacccgaagg
    12601 tggacatccc ggtcatcgtc atctccgctg cctaccgggg agcaagcccg gccgagatcg
    12661 aatccctcgt cagcaagccg ctggaggatg ccgtttccga ggtggacggg gtggacaaga
    12721 tcgagagcta ctcgatcgag ggcgtttcct ttgtcgtcgc ggaaatgaag ctcgacgtga
    12781 acctttcgga agccgtcgtg gacgtttcca accggatcaa ggcggcccgc tcgaaactgc
    12841 cggccgaggt ggacgagccc gtggtcgaaa aagtcgacat caacgcggag ccgttcatgg
    12901 tggcggtttt cacgacggac atggacggaa agaaggcgcg ggacatcctg gaagacaagg
    12961 tcagaaggcg cctcacccgc gtggagggga tcgcccaggt ggccctggtg gggggagagg
    13021 aacgcgagat ccatatcctg ctgaaccctg tcgcaatccg gaatttcaac ctttccccgg
    13081 ttctcgttgc gtccatcgta gcggccaaca acctgaccaa tccggccggc tatatcgccc
    13141 aaaaagaccg ggagatcacc cttcgctaca aggcggaagc ccgcgatgtc aaatctctgg
    13201 gtgagatcca gatcccccta ggcggagggg cctccgttcg cctgggggat gtagcccgga
    13261 tcgaggacgg agcaaaagac ctgcgggaat tcgcccgctt tgacggcaaa cccgcgttcc
    13321 agctccagat caaggccagg ccgaacgcga acatcgtgga ggttggggcc ggcgtcagga
    13381 aagaactgga aaaaatcctg ccgacccttc cgaagggctt ccggctcgac attccctacg
    13441 ataactcgaa gttcgtctcg aactctgtcc ggaacgtcat ccgcgacatg atcaccggga
    13501 cccttctcac ggccctggtc ctctaccttt tcctccggag gctttccgcc acggcggtcg
    13561 tctccatcgc catgcccacc gccgtcctgg cgacgttcat cccgatgatg atccagggct
    13621 acaccctgaa catgatgagc acgctagggc tggctctttc catgggggtc ctggtgaaca
    13681 actccatcct ggtcctggag aacatcacgc gctttcggga catggggtac gatcccatcg
    13741 aggcggccga gcgcggaacc gttgaaattg ccctttcggt gctggctacg acagggacca
    13801 acctgggggt tttcctcccg gtggccttcc tgccgggagt agcggggcag tttttggttc
    13861 agtacgccat ggtcattgtc tatgccacgc tcttttccct gtgggtttcc ctcaccctga
    13921 cccccatgct ggcggcccgg atgccccaga atccccccac tcccctgtcg tgccggcttt
    13981 gcggctggtg ggaatggctt tatgaggggc tggagagcct ccatcaccgg ctcgtggtgg
    14041 ccgccgtcag ccatccgtgg aaaacgctgg ttctcacggg actgcttctg gccgcggttt
    14101 tcgcgctggc accgcgcctg gggtccgagt ttttcccccg ggtggacgaa ggggtggtat
    14161 cggtgagcgt cgagctgcct gccgaaagtt ccctggcccg gacggagcga accgtccggc
    14221 ggatcgaaga attcgtcctg aagcagccgc acgtgaaaaa cgtcgtggtg tccgtgggag
    14281 ggaccggagc aaggaccggc gtcaatctcg ggagcgtgga ggcctacctg gacgaggatc
    14341 cgaaacggcc ttccacgttc gagttcgcag accggatccg ccctttcctg gcttcccttc
    14401 cggacaccac cgtgagtgtc caggcggtca agaccagctt ttccaggggg aaacccctgc
    14461 agatcgtcgt cacgggggag gaaaccgggg tcctcagcca gattgccggg gaggtcatga
    14521 aggtgatccg gtcggtgccg ggggtcgtcg acgtggacct ggactggcgg gtcggcaggc
    14581 cggagctggc gatggaccct gtccgatggc gcatgggccg gctgggatcg gacgcggcgt
    14641 ctgtttcctc caacatccgg ggatacatca cggggctcga ggccgggaaa taccgtgaag
    14701 gcgggctgga atacgacatc ctggtgaaga tggagccgca gcggacccaa agcgttttcg
    14761 ggaccccgga cctcccggtg atgacctccc agggagcggt tcccgtaaag gagatcaccc
    14821 gtttttcgta cgcggccggc cccacgcgga tcgtccggaa ggaccgcgtc cgctcggtga
    14881 ccgtggaggc cgacgtggtt ggccggaccg tgggcgaggc cttccaggaa atccggtccg
    14941 gcttgagggc cctgccggtt ccgcccggct acgccgtgaa gtacgagggg gacgtcgagg
    15001 cgatgcggga aaccttcacg gacatggggg tggctttcgg cctcgcggtc ggaatcacct
    15061 tcctgatgat cgcggcgatc ctggaatcgt gggctttcgc gttcatcatc atcctgacgg
    15121 ttcccctttc cgccatcggc gtcatcccgg ccatggtggc gaccaacacg gcagtcagcc
    15181 tgtacgcgct catgggattg gtcatgctgg tgggactggt ggtgaacaac gcgatcgtga
    15241 tcgtcgattt cgcggagggc ctccggagga acggaacggc agcggccgaa gcgatcaccg
    15301 aggcttgccg cgtccgtttc cgctccatcc tgatggctga cttgacctcc attttcgcca
    15361 tgatccccct ggcgctgggg atgggagaag gaggagccct gagggcgccg atggcgatcg
    15421 tcgcgatcgg cggtcttttc gctggaggcg gattggccct gttcgtcatt cctccggtct
    15481 atcggctttt ctgggaacgc cgggagcgcc gagcgcttcc cgcccgaaga agattgcggg
    15541 atcggctcgc gggctggttc ggcaggttca ggggttgagc ttccagatcg aggcgttgag
    15601 gatcgccgca aagctgaccc acgccaggta ggggaccatc agccaggcgg ccagagggga
    15661 aaccggtttg aacgctgcaa gggtggccag gatggcggcg gccagggcca ggatgtccac
    15721 gagggccagc aggggggacc tgaggccgaa aaagagggcg gaccacaggg tgttcaggga
    15781 aagctggatg agaaaaagga aaacggcgtt gcggatgttc gggtcggcca ggcttcccct
    15841 tcgccagacg agccaggcgg cgatgcccat gagcgtgtac agcaccgtcc atacaggccc
    15901 gaaaacccag cccggggggg tgaaagcggg cttgacaaga cccgtatacc acgattgcag
    15961 gctagaagcc gtgaaaaacg agccaaggat ccccgcggcc tggcagacaa ggatgcaggc
    16021 ggcgaggcgg agaaagtcag ggcgattcag ggaaatcccc cctttttgca attgggttga
    16081 agcgatcata gcacgaacgt cgtcgagccg gtcgggaggg aaagccggtt gaaaatcgag
    16141 tggaacgatt ccgaagaatg gggcaggttt tgcaaggggg ccctttctcc cgcggaggct
    16201 gctgcactgc aggtccggct ttcggggcgg gtttccctcg aggatgcccg aagcgggttg
    16261 ccctcgaccg tggcgggcgt ggacgtgtcg atccggagag gccccctcgg ggacgtggca
    16321 gttgcctgca tcgccctgat ggaatgggaa agctgggaac ttctcggggt tttcagggcg
    16381 gaggaaccgg ttcgttttcc ctacatcccg gggcttctct cgttccgcga actccccgtg
    16441 attctcaggg ccgccgaagg cctccccgag ccgccgggcc ttttcatggt ggacggggcg
    16501 ggaatcgccc acccacgctt tttcggcctg gcgtgccacc tcggcgtcgt tacgggctgt
    16561 ccttccgtcg gggtggccaa gagccgcctg gtgggaaccc acgatccgct tcccggggag
    16621 cggggatcgc gcgttcccct tcttgtcgag ggaaaggagg ttggaaccgt tctccgaacc
    16681 cgccggggag tgcgtcccct ttacgtgagt ccgggccatc gggtttccct ggggaccagc
    16741 ggggatcttg tcctcgctgc ctgcacgcgc ttcaggcttc ccgaaccggt tcgggcggcc
    16801 cataagctgg caggggatca aactcttctg tattcttaat acccggaaca cttgcaaaag
    16861 acccggctgt cgtcgatgat acgaaagaaa accaaccttc caaaatttaa aagaggtgac
    16921 ctgtgcgtgc tcattacgat cgcggatccg tttgatccct cccttcccgg caagctgggg
    16981 cagttcggcg aagtggccac cggcctggac cggcttgcgg aaaccgatgt cctcctggtc
    17041 cggagcaaga cgaaagtgac ccgggaaatc ctggaaaagg ctccgaagct gaagctggtc
    17101 atccgtggcg gcgttggaat cgatacgatc gataccaagg cggcggaaga aaagggcgtc
    17161 cgggtgagga ataccccgag ggcttcctcg atcacggtgg cggaattggc tttcgcgctc
    17221 atgctggccg tcccgacgcg cctgatcgag gcccacatca gcatgtgcca gggcaagtgg
    17281 gagaagaacg agctgaagcg aaccgagcta ttcggaaaaa ccctcaccct ggtcgggctc
    17341 ggcaacatcg ccgcggaagt cgccaagcgg gcccaggcgt tcggcatgac ggtctgcgcc
    17401 tatgaccccc atgtccctgc gcagagggcc gaggagctgg gggtgaccct cctgggcgac
    17461 ctgaaagagg cgctttccag ggccgactac gtgtcgctcc atgtccccct cgtggagagc
    17521 acgagaaacc tgatcaaccg ggaaaccctt tcggtcatga agaagggcgc ggtcctggtg
    17581 aacaccgcaa gagcgggcgt catcgtggcc gaggacgtcc ttgaggcgct cgagagcggt
    17641 cagctggcct actactgcgc ggacgtctgg ccgacggatc ccccttcccc ggactatccc
    17701 atcctgaagt ccaaccgggt cttcatggtg ccgcacctcg gagccaacag caaggaaaac
    17761 cttctccgca tcggcgagga ggtttgctcc atcattcgcg aagcccaaaa ggaaggagtg
    17821 ttgtaagatg ccccgcaagc tgaattttca cgcaggtccc tcgacgctgc ccgtggaggt
    17881 cctggaggaa ctccagaaga acatggtcga ctttgaggga acgggtcttt cgctgatcga
    17941 agagagccac cgcagcgcca cctacgaggc tgtccacaac agggccatcg aactgttccg
    18001 cgagctcttc tccgtgccgg agaactacga catcctgttt ttggggggag gcgcgaccct
    18061 gcagtttgcc atgatcccca tgaacttcct gccccaggga aagacggccg agtacgtcgc
    18121 caccggaacc tggtcgaaaa aggcgatcga cgacgcgaag aaactgggga gcgtcaggat
    18181 tatcttcgac ggcaaggaaa gcggctacac gaccctgccc gaccccgcct ccgtgaaagt
    18241 ggaccccgaa tccgcctacc ttcacatcac ggccaacgag acgatcggtg gtctcgagtg
    18301 gaaagactgg cctgagaccg gcggcgttcc cctcgtttgc gacatgtcca gcgacatcct
    18361 gagccgcccc gttcccgtgg agaaattcgc cctgatctac gcgggggccc agaagaacct
    18421 ggggcctgcc gggacgaccg tcgtcatcct ccgcaaggat atgctcgacc ggatcccgga
    18481 caccctgccg gcctacctga gctacaagaa ccacgcggcc aacaactccc tgtacaacac
    18541 gccccccgtc ttcgccatct ggacgatcaa gctggtcctg gaacacatga agaaaaacgg
    18601 agggctcgcc gcggtggctg agcggaacct gaagaaggca gggcttgtct acggggtcat
    18661 cgattccagc ggggggttct accgttcgcc ggttgacgcg cgctaccgct ccaacatgaa
    18721 catcgttttc cgccttccct ccgaggagct cgagaagaag ttcatcgacg aagccaaggc
    18781 cgcgggcatg atcggcctga agggacaccg aagcgtcggc ggctgccgag cctctctcta
    18841 caacgccatg accctcgaag gtgccgaggc gttggcggcg ttcatgaagg aattcgccgc
    18901 gaagaacggc tagggagtcg accatgggag acgttagagc caggctggaa aaagtcggca
    18961 tcggccttcc ggagatcctg ctcccgaaag cggggacgga cctgtcgaaa tggtccgttg
    19021 tggcctgcga ccagttttct tcggagcggc actactggga gaaggtcagg gaaatcgtgg
    19081 gcgaggctcc ctcgacgctg cgcctgatcc tgccggaatg ctacctggag gacggagata
    19141 cagaagcgcg tgtcgaggcg atcaatgccg agatgaagag ggtcctcgaa accgtcctcg
    19201 agcccctgga gccggggttt gtcctgattg accgaaggac tgcccacgcg ccttcccgca
    19261 aggggctggt cctggcggtg gacctggaag catacagctt tgaagcaggt gctgaatccc
    19321 tgatccggcc cacggagggg acgatcctga agcggctccc gccccgcatg gccatccgcc
    19381 gcggggcgac cctggacctg ccgcatatcc tgcttctcct ggacgacccc cagcggacgg
    19441 tgatcgagcc gctcttctcg gtacgggaga acttcaaaaa gctctacgac ttcgaactca
    19501 tgcagggggg cggctccatc gcgggatggc acttgcccgc ggaggccctg ggggatacgg
    19561 ttaccgcgct ggagaaactg gcctccggcg gattcctttt cgccgtgggg gacggcaacc
    19621 attcccttgc cgcggccaag agcctctggg aggagcggaa ggccgcaggg gcgggcccgg
    19681 atgacccggc ccgctgggcc ctcgtggagg tggaaaacat ccacgacgag ggcctgcatt
    19741 tccaccccat ccaccgggtt ctcttcggcg taagcgtgga tgccgtactg gacgaaatga
    19801 agaaggccct gggaggaagg atggaggccg gaaccgcaaa ccccgctccg aaaaccgaag
    19861 cggggcacgt catcggcgtg gtctccgaaa agggggaagg tcggtttgtc ttcaagccga
    19921 aggaagagcg catgaccgtg gagttcttcc aggaattcct ggacgaatac ctggcgaagc
    19981 accccgaggt cacgatcgac tacatccacg gggaggaagc cgtccgggaa atgggctcga
    20041 aacccggcaa cgcgggtttc accatgccgc cgctggacaa gtccgtcttc ttcaggcgca
    20101 tggtcgaggt ggggccctac ccgaggaaga ccttctccat cggcgaggcc accgagaagc
    20161 gctactacct cgaaagccgg aaactggtgc ggtaagaaga aggaccgcga gagcacgttg
    20221 cggggagcct gtgggttccc cgcttttttt tgcccttgcc ccggaagcgg tgagtggtgt
    20281 tatacttttt atgatgtttt tttaatgcgc tattatctcg atagggacga tttcgaaagg
    20341 gggctttgtg gatggggaaa atgcgtggac ggtttcttct tttgctcgtc atttcaatgc
    20401 tggcctttgc cggggcggcc tgagggttga gcaccctttc cctggattat cctggagagg
    20461 gagccgaatc ggtctccacg tccttcaatt tccagtggac cctaacgccg gacgtggatg
    20521 tgacgagccc ggaccttgtc agttacgacc tttacctttc caagacgctc tcggggcttg
    20581 acgcgggcgt gctttcccag gacctgaaat ttgacggata cgaggggacg ttcccttccg
    20641 gtaccctgga cgaagactcc ctttatttct ggaaggttgt cgctcacacc ccctctggag
    20701 acgtgggcgc gacgagcttc ttcatgaccc agggcgaggg cgccagcctg gtgctgaaga
    20761 gcccgtcaaa cggccagacc ggggtcgctc ctcagaacgt ggagttcaag tgggaattcg
    20821 attccgggga caatagcctg acggcggagg atgtcacctt cgacctgtac ctctcggaca
    20881 agtacgagac cattttcgac ggggctccga agaggtcggg gataaaggcc ctagatcctg
    20941 acgtcgttga tggggtcttc agcgtgacga tcaaccagtc ggtcggttac ggcaagcact
    21001 actggacggt cgttgcccat gtggacggag atggcgattt taccacctcc ccgcattctt
    21061 tcacgaccct gagcgaagcc tccgacccga acctctcggg cggctgctcc gcggctccgc
    21121 tgggcggcct ggctgccatg gtcctgctgg ctggactggt cctggtgaag cggggcaggt
    21181 agagagaagc atttaggaag cgaccgagac gaaaacaggg acccgggcga cgcccgggtc
    21241 cctgtcgctt tcctcttggc cgaaggattt cctagaagct ttcctgggcc gtgcgggcga
    21301 tgatctcctc ctggtggtac tggtcgaggt cgacgaagta gtcgctgtag cccgccaccc
    21361 ggacgagcag gtcccggtag ttctccgggt gtttctgagc gtccctcagg gtgtccgaat
    21421 ccacgacgtt gaactgcatg tggtgcccgc ccaggcggaa ataggtccgg atcagctggc
    21481 aaagggcatc gatgccggac tcccccgcca ggacggaagg caaaaacctc tggttcagca
    21541 gcgtgccgcc cgatttgacc tggtccatct tggccaggga cttcacgacg gccgtcgggc
    21601 cgtggcggtc cgcaccgtgg gacgggctgg ttccgtccga ttcgggcagg aaggcgaaac
    21661 gcccgttggg agtcgcgccg agctttttcc cgaagtagac gtggcaggtc gtggaaagca
    21721 tgttcaagtg gtacgtcggt cccttgggac tgtgctgccc ctcgatggtg cggaacaggg
    21781 aatcgtagac gcgctgcatg atcgaatcgg cgtagtcgtc atcgttgccg aagaaggggg
    21841 tcttgttcca gacgaactgc ctcaggggtt cctctccctc ccagttcttc ttcagggcgt
    21901 cgagcagggt ccgcatggtg atcgtgttgt cctcgaagac gtgcttcttc aggaccgcga
    21961 ggctgtccgt gatggtgccg atgccgcagc actggatgta gtcggagttg tagcgcgccc
    22021 cgccgtcgta gtagtccttg ccgttcttga tgcagtcgtt gatcaccacg gaaaggaagg
    22081 gagcgggagc gtaggtggcg aacatgcgct ggaggtagtt gtcggtttcg atcttcgttt
    22141 ccacgacata ccgaagctgc ttctcgaagg cggaatacag ctcgtcgaag gtggtgaacc
    22201 cttccgggtc gcccgtttca aggccgacct tcttcccggt gagggggtcg acgccgttgt
    22261 tcagggtcag ttccaggatt ttcgggacgt tcaggtagcc gtggaggatg tacgcttcct
    22321 tgccgaaggc gccggtttcc acgcagccgc tggtgcctcc ttcgcgagca tcctccggag
    22381 tcttgccgac ccggatctgc tcggcgatga ccatgtcggc gttgaacacg gaaggatacc
    22441 cggaaccgcg gcggatcacc ttcgcggcga acttcaggaa cgcgtccggg gtcttcgcgc
    22501 tgatctggac gctcacctgg ggctgcagaa gctggagttc gtcaagaacc tccaggatca
    22561 gggttgagac ttcgctcgat ccgtcggttc cgtctgcctt cagtccgccg agattgatct
    22621 gggtgaagtc gttgtaggtt ccgctttcag cggccgtgac gccgaccttc ggaggagccg
    22681 gggtgttgtt caccttgatc cagaagcagg aaagcagctc gcgggcccct tcccggtcca
    22741 gggtcccctc ggctaggtcc ttctcgtaga agggagccag gtgctggtcc aggtggccgg
    22801 ggctcatggc gtcccagccg ttgagctcgg tgatcgttcc gagatggacg aaccagtaca
    22861 tctggagggc ttcccagaaa ttccgcgggg catgggcagg gacccaccgg cagacggaag
    22921 cgatcttcag gagctcggcc ttccttttgg gatccttttc ctcggcggcc atcttctccg
    22981 cgaggtcggc gtggcgctcc gcgaagagga tcgccgcgtc gcaggaaatg tccatggcgg
    23041 tcagttcctc gaatttctcc gtggcgtggg ggtcgttcag gtaatcaagc ttcgccttgc
    23101 tctcggcgat ctcccgcttg aagtcgagca tccccttgcg gtagatcgtt ccgtccaggg
    23161 ccgtgtgtcc cggggcccgc tgctccatga attccgtgaa cagcccggcc tcgtaaaggg
    23221 ccttccactt cgcggggatc tgggcaaagg ccttgtctcg catgctgcgt ccgcgccagt
    23281 agggaatgac cttctccgcg tagaggtcga tgtcttccgg ggaaacggag tagctcgtca
    23341 tcttcctgga gttgaggatc tccaggtcct cccgggaatg acagttcagc tcggggaagg
    23401 tggaaacggc cttgggagcc ggtccccgtt ctccgacgat cagctcgtcc gggccgatgt
    23461 agatcgtctt tttctcgcag agattcttga agttcaaggc gcgcatgacg ggagtcgagt
    23521 atttcccggc gttttcctgg tagaactcgg tgaccagaag agcccgctcg atggaaatgg
    23581 acgggtgggt ctcgaagctt tcctttctga gtctggcgat gcggtcgttc atgcctttca
    23641 ccctcctatg aggacattga gtccaaatga cttcaggttt tctgccgctg cttcctgggc
    23701 ctccgcgctg ggcgggaggg tttcgggaag ccggtaggcg atcttccact tcgcgtactt
    23761 gttgcgcccc gccgcatggt acggaagcag gtggactgaa acgacgcctt tcagcgccgc
    23821 gacgaaccgg cctgtctgtc gcaggttttc ttcatcgtcg ttgatgcccg ggatgagggg
    23881 gattcggatg gcgacccggg cccccgaatc ggtgagggcc ttgaggttct ccaggatcag
    23941 cgtgttgtcg acccccgtgt agagcttgtg cttcccggga tccatgtgct tgatgtcgta
    24001 caggaaaaga tccgtgagac gagccgtttc caaaaggact gccgtctgag cgaagccggt
    24061 ggtgtccacc gcggtatgga tctgggactt gcggcagcct tccagcagtt cccagaggaa
    24121 ttcgggctgg aggagaggtt caccgccgga aaaggtcgcg ccgcccccgg attcgtcgta
    24181 gaaggggatg tccttcctga tgacggtgag gacctcttcg gaagtcatga gacgcccaac
    24241 cctttcccgc gccgtggacg ggcatgcgtc cgcgcaggcc ccgcagccaa cgcaccggga
    24301 aaagtcggtc tcgaagcctt ccggcgttgc gttgatggct ccgttaggac aggctttcag
    24361 gcaggctccg cagccgatgc accgctcggg cctgtagagg acggcaggct ggggggaaat
    24421 gctctcgggg ttgtggcacc accagcagga aagcgggcac cccttgagga aaacggtcgt
    24481 gcggattccc ggaccgtcgt gaatggaata ctttttgaag tcgaacacga tgccgctttt
    24541 catgacaggt tccccttctt cgcttgccca ctctcggggc tttcgctgat acatcatata
    24601 tcagtacctg cgacccgtca agcctttcgg cgaaaatccg cggttttcct aagttcgagg
    24661 gcggaccgca gcatcaggat tccacggatc tcccggaggt cgagccccgt caggcgtccg
    24721 atcttctgga gccggtacgc cagggtgttt cgatggatgg aaagggtttg ggccacccgg
    24781 gtcggttcga aagggctgtc gcaccaggcc aggaaggtct ggatcagttc ttcggcatcg
    24841 gaagccccgg ggagatcctt caggacggat tcacagaagg ccgccgcgcg gtccttggga
    24901 acagctgaaa ggagggactc caggcgcaag tcgccgatgt agaagacctt ttcctctccg
    24961 cacttcttga gggatagccg ggcggcctcc ccggcagagc ggaaggcctc gcccagggaa
    25021 gccgcggaag cggcgatttc gccgatcccg atccttgccg aaagcccctg ctcttccagg
    25081 gaaagagcca aaagccgggc ggtatttctg acctcctcaa gaggctggat ctcccgcacg
    25141 gcgagcccca ggaaggcaag gacgacgatt ttgtcatccc cgaggcggga gacgatgtcc
    25201 tgtgggtgag aaaagaatcg ccgggccaag gcctgtgcct ccaggcgcag gttcaggggg
    25261 gctttctccg ggtgaaggat ctcggcgacg agaacggccc tggggggtct caggtcgtag
    25321 ccgagttccc ggccgcgcat caccagcagc gtttcatccg ctttcagggg gtcgaaagcc
    25381 gcgatttcca ggatcagttc ctggagggcc tgttctctca gcatcgaaga gcggagaagg
    25441 gcattctccc ggagcaggag ttcggcctgt ttctggacca gccgcccgta ccgggagacc
    25501 tcctcgggcg gacccgcgat ggcgatggag ccgacgaccc ggcccgcaag ggaaatcggg
    25561 agggtgatcc cgggccggac tccctccggc agccgatggg cctcctccgg ggtggtgatc
    25621 tcgggtttcc gcgttcggat cactcccagg gaggggctgt gaagggaacc caggcgcgaa
    25681 gggtcactcg agccgatgac gatcccgttt tcgtcggtta acaggacgct gtacccggcg
    25741 atttccgagg tgctttccgc gagttcctgg gcgcagcttc tcagcatttt tccagccttc
    25801 cccccctttt tgtcgaatgt gcatttgcac aaaattcggg tggaaaacat tgccattgta
    25861 ggtcgattgt ccaatgaaag cgagagggaa aaacgggtac actacctttt gggagttcct
    25921 gctgtcagtg cgtcagttcg aaacgtcaga gaaaaagcag gacgagcgaa aggaggggcc
    25981 tggatgaggc tggagctgca cgaagtccat gtttcggata tccagtgggg agagaggacg
    26041 gaggtggtcg accatgtcct tcgggtgaac cgcgaagaag ccgcggctgc gctcgccggg
    26101 gaggatcggt tcgcctcggt tcggctggac ctggcacgcc cgggagagag tgtccggatc
    26161 attccggtca aggatgtcgt ggaaccgcgg gtcaagctga agggcggaag ggggtatttc
    26221 ccggggttct tcgccccgcc ggaaacagcc ggcgagggag tcaccctcgt cctgaagggg
    26281 gcggccgtgg tgacctgcgg cccgatcgtg gctttccagg aggggttcat cgacatgtcg
    26341 ggaacgggcg ccgattatac gccgttctcg aaaacgaaca acctcgtcct gacggtcgaa
    26401 ccagtcgagg ggcttggcaa gcacgcttac gaggaggccc tgcgcctggc gggcctgaag
    26461 ctggccctgt accttgcgga agcgtgccgg gaggcgaaac cggacgaaat ccgggtcttc
    26521 gaaaagggat ccatcaccga ggagaatgaa aagtacccga acctgccccg gattctttac
    26581 atctgcatgg ccatcaccca gggcctgctg cacgacacgt tcctctactc cgcggacatg
    26641 aagagcgtcc tgcccaccct cctccacccc aacgaggtcc ttgacggggc gctcgtctcg
    26701 ggcaactgtg tttcggcctg cgacaagaac acgacctacc atcacgtcaa caacccggtg
    26761 gtggaggcgc tgtaccggcg ccacgggaag gacttgaact tcctcggcgt cgtccccacc
    26821 gtggaggatg tccggctggc cggcaaggag cgggcttctt cgatgaacgc ggcgctggcc
    26881 aaaaatctcg gggctcatgg ggtcatcgtc acagaggagg ggtacggcaa cccggacacg
    26941 gatctctgcc tgaacgcgaa gaaatgcgag aaccttggga tccgtaccgt tgtgatctcc
    27001 gacgaggcgg cgggaacgga cggcgccagc cagagtctcg cggacgccac cccggaaatg
    27061 tccgccttcg tttcctccgg gaacgtcaac gagatgatcg aagtgcctcc catgaagacg
    27121 gtcctcggtc acccggaagc catcgcgaac ctctccggag gggcggccga aagcctgcgt
    27181 cccgacggaa gcatgttcgt ggaactacag gccgtgatcg gctccacctg cgagctgggt
    27241 ttcaaccgca tcggctgcga atggatctag gcgagggagg aatgagcacg atgaccatca
    27301 gggttgtcca ttacatcaac cagttctacg cgggacttgg tggcgaggag gcggccaacg
    27361 cccttcctga aagccgaaag ggagccgttg gccctggagt gcagcttcaa acgcttttcg
    27421 gcgagaaggc ggaaatcgtc gggaccgtca tctgtggaga cgggacctac ggcgagcgga
    27481 cggaagaagc acgagcggat tgcatccgcc tcatcgaggc tttccagccg gacctccttg
    27541 tggccggccc cgcctttaac gcgggccgct acgggttcgc ctgcggggac atctgttccg
    27601 ccgccggcaa gatgctgggc attcccacgg tgaccgccct ttacccggaa aacccgggag
    27661 tggaactttt cgcgaagaat accgtcattg tccaggccgc tgacagcgcc cgagggatga
    27721 aggcggccct ggaagggctc gcccgcgtcg gcctcaagct tgcctccggg gaaccgctgg
    27781 gcccggccag gctggaaggg acgatctcca gggggatccg gaagaatttc ttcttcgaga
    27841 agaacggggc cgagagggcc gtggagatgc tgctggacaa gatcgcaggc aggcccttcc
    27901 gaacggaata cgagatgccc gtcttcaagc ggatcccgcc ggctcccccc atcaaggacc
    27961 tgaagacagc caccatcgcg ctggtctgct cgggcgggat cgttccgaag gggaacccgg
    28021 accatattcg cgtgtcttcg gctgaaacct acgggcggta taacatttcg gaattgaacg
    28081 acctgacccc ggaaacgtac gaatcgatcc atggcgggta tgaccgggtc tgggcgaatc
    28141 aggacccgga cgtggtcctt cccgttgacg tgatgaggga actggagagg gaaggggtca
    28201 tcgggaagct gtatgattgg atctatacca ctacggggac cggaaccgcc gtggcccacg
    28261 cggagcgttt cgggagggag atcggggcgc agctgaaaga ggcgggcgtc gacggggtga
    28321 ttctcacctc cacctgaggg acctgcactc gatgcggcgc atcgatgacg cgagaaatcg
    28381 aaaaggctgc agggattcca gtggtccaga tcgccacgat cgtcccgatc atgctcacgg
    28441 tggggtccaa ccggatcgtt cccggcgtgg ccattcccca tcccgtggga gatccggcga
    28501 aggggcccga ggcggacaaa aaggtccggc gagaactttt gctgaaagcc ttggaagcca
    28561 tgacgacccc tatcgagcaa cagacccttt tcgaaaggaa gtgagcctct ccaagcccat
    28621 ggtgagaaga tcggaaactc tgatcccggt ttcgaagaac ccgccggcca aggtgaagga
    28681 caaggggctg atccgcctgg cccgctccgg tgcggctgcc tacgacgctt cggcagacag
    28741 gatcctctgg ctgccctggg gagtctccct cctggaccgg gtaagacgcg ggctttcttc
    28801 acggtttcgg tctgccggag cacagcccgt tctctgcggc caggccccct ctggaaccct
    28861 gccggttgcg gtgcgggtcc tcaagactgc cgaccagctt cctttgaccc tcatggagga
    28921 agacaggacc ctgatcctcc tcgaaggatt tgacccggaa ggagctcatc cgggctggcc
    28981 ggtttacgcg gaacggctcc tctccttcct ccaggaaagg gggatggagc tctcggtgtt
    29041 ccaggaagcg ctgcctttcg gggaacgcac gctggttgcc tgcagctgcg aaggggaagc
    29101 caggggggcc ttggaggcaa gggtctgtcc tgcctgcggg tggaccgctt cggtggatgc
    29161 cctgctccca ccggtagagg aagcggacga ggaggaacgc ccgctggagg cggtgctgac
    29221 gccgggcgcg acgacgatcg cggagctttg caggcagctt ggatgtcctc cagaccggac
    29281 gatcaagacc atgttctatg cagcggaaaa agacggtaag acgttcgtgg cggcggcgct
    29341 ggtgcgggga gacagggccg tctgcccgac gaagctgtcc cgtgccctcg ggggtgcggc
    29401 ggttcggagg gcggaaagcg ccgatctcaa acaggcgggt tgcgacgtgg cgggtttcct
    29461 ggggcccgtg ggcctgccgg agaccattcg ggtgattgcc gatgcttccg tggccggcac
    29521 gaaaaaccgc gtgatcgggg ccaaccggcc cgaaacccac ttcaccgggg cttgctgggg
    29581 aagggattac tcggccccgg tagcggacat ctccgccctg gaaccgggag gcgcctgccc
    29641 tgcctgcgga agaccggtag aaaagggatg gatccggccc ttggcggagt ttgccaaggg
    29701 acatccggcg ctggcggcct tcccctcgtt gggctacctg gacgaaaacc ggaagcgccg
    29761 cctgcccggg acctggacag gaagaatcct gctggaggcg atcgccctgg cctcggccgc
    29821 ggaaggcgac gcgcccttca ggcttggact gtcgcccgcg gacctgctgg tcatccctct
    29881 tggcgcggga ggacgggagg cgtcgaaagc tgccgaagcc gtggcggacg aactggaaca
    29941 ccggggctgg accgtccttc tggacgaccg gccgcttccc gacgaagcgc gtctcgctga
    30001 agccagtctt gccggttgtc ccgtgcaggt actcgtggcg gacggactgg aggctgaagt
    30061 caccggtgtc ggaggaggtc cagggaagcg ggtccccctg gagggaatcg ggggtttcct
    30121 ggagccgctg cggggagcgt gagtcccagg cgaaatgatt cacagggggg cagtgcgaag
    30181 gcactgcccc cctgtgctat tgatatttca gtctttcaaa gcggtgtggc ttgactgcat
    30241 taagcccgct cccctatact c
//
LOCUS       DAOOVN010000012        29156 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_1093_length_29156_cov_14.013848, whole genome shotgun
            sequence.
ACCESSION   DAOOVN010000012 DAOOVN010000000
VERSION     DAOOVN010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 29156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29156
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1093_length_29156_cov_14.013848"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PLY83_02510"
     CDS             <1..294
                     /locus_tag="PLY83_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV28940.1"
                     /translation="LGLSVTQKNYPGALFFAAGDYHHHVGTNIWDSRNAPPPPPQAAG
                     LRRFSLILPAREDVESLKNRTLQSGYPIETLPAEGGFLLRDADRIAVGIEALP"
     gene            291..1451
                     /locus_tag="PLY83_02515"
     CDS             291..1451
                     /locus_tag="PLY83_02515"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOV28941.1"
                     /translation="MSEFGRRLSLLIAAAFGIFAYSNVYYFLANLLGSRGVSPQAAGV
                     AVSLFYVATTVFRPAGGWLVEGFGLRPTLVGAALVAFAGSVGIAFAGSNLSLVFACRI
                     LMGLGYSAFIVAFTAYQNLFVPPEKRGFAFALMTAGSMVPHVSIIPAADWLIRHGHVA
                     AYLSLSPLMALFCAALGIAFGKVSLAKGASRKEAWGSYRDLFSLAPVRALLASMFLLS
                     LTDSMVLCVGALLVPRGLVPSAFLVSSAAAALAVRIATLHFVDRIPRPRIAAPSFGLM
                     ALAMAILSLMRSNAGAALCGAMFGLGVGMGFPAHLAMVGDMTPERLRPKATAMVWFAM
                     DSGWAVTPFVFGLLSPVLGDSLAFGGFAAVMLAGAAGAWRFLWRPVLAEEKS"
     gene            complement(1506..1841)
                     /locus_tag="PLY83_02520"
     CDS             complement(1506..1841)
                     /locus_tag="PLY83_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV28942.1"
                     /translation="MRGRRSSAAARLGCMRQFLLLAILWGVFVAFNVLTRRFAPGLEL
                     RIGQISVTAFAVSAAELFAAWWISLRVTNAVMNRLIARAVAREEELERLEAGKAGAGA
                     AAENPATEE"
     gene            2049..2288
                     /locus_tag="PLY83_02525"
     CDS             2049..2288
                     /locus_tag="PLY83_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV28943.1"
                     /translation="MLFGSFVEIGTQAIRKEAFDINDNLMLLLFGDFLGIPNPISYYT
                     LELLPYVAEELVPWERRMQNRKTIVAEKAGQYDFG"
     gene            2299..3246
                     /locus_tag="PLY83_02530"
     CDS             2299..3246
                     /locus_tag="PLY83_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HOV28944.1"
                     /translation="MTRPAYRPFSFFGGKGGTGKTTCAAARALALSEEGIKTLVVSTD
                     PAHSLADAFDLSIGEEIGQIAPNLWAIEIDAETEAQKYMKGIQEKMLHIVSPVIVDEI
                     KRQIEIAYSSPGAEEAAIFDKFIELMEQLNSPYEAIVFDTAPTGHTLRLLTLPEILGA
                     WLDHLLEKRARAVELMSMVAKYDREVRQKLESDPITDILGKRKAKFETARRILTDPAL
                     CAFYFVLNAEKLPILETERAVRLLTKYGIPIGGILVNRVIPEGAGDFLEKHRLAQQGY
                     LRQIRETFASLPVTEIPFLETDIRGINELDKITGLLKNL"
     gene            3289..3753
                     /locus_tag="PLY83_02535"
     CDS             3289..3753
                     /locus_tag="PLY83_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HOV28945.1"
                     /translation="MGGNMNLRSPAFDEGGPIPAVCGYKKGNVSPPLRWSLSPAGTRS
                     FALLVEDPDARGWVHWIAWNIPFSASELKEGVPPTPQLPDGTRQGLNDFGKIGYGGPC
                     PPSGVHRYTFRLMALDTVLDLPPGSERYDLMKAVDGHVLEETRLTGTFSANS"
     gene            4001..7531
                     /gene="nifJ"
                     /locus_tag="PLY83_02540"
     CDS             4001..7531
                     /gene="nifJ"
                     /locus_tag="PLY83_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOV28946.1"
                     /translation="MATRPMHTLDGNEAAAYVAHKTNEVIAIYPITPSSPMGEWADEW
                     SAKGIKNIWGTVPLVSELQSEGGASGAVHGALTAGALTTTFTASQGLFLMIPNLYKIA
                     GELTSTVFHVAARTVATHALSIFGDHSDVMAVRASGFGLLASNSVQEVMDMALIAQAS
                     TLEARVPFIHFFDGFRTSHEVSKVEMLTEDDMRAMIDEELVKAHRARGLSPDRPVIRG
                     TAQNPDVFFQAREACNPYYDKAPEIVQRAMDKFASIVGRQYHLFDYVGAPDAERVLVM
                     MGSGAETAHETVDYLTARGEKVGLLKVRLYRPFSVSHFLETLPKSVKTIAVLDRTKES
                     GAIGEPLYLDVVAALKGRPDVKIFGGRYGLSSKDFTAAMVKAVFDEAAKLEPKNGFTI
                     GIDDDVTFRSLPYDPSFSTEDPKTVRCLFYGLGSDGTVGANKNSIKIIGEDTPNYAQG
                     YFVYDSKKSGSMTVSHLRFGPNPIRAIYLIDRANFVACHQFAFLEQYNVLKSAEKGGT
                     FLLNSPYGPDEVWDHLPRTIQKEIIDKNLKFYVIDAVDVAQKAGMGGRINTVMQTCFF
                     AISGVLPKDEAIAAIKHSIEKTYGKRGPEIVKKNFEAVDSSLAHLHEVKVPGTVTSTF
                     DIRPPVPAEAPEFVQKVTAKIIAGEGDSLPVSALPADGTFPTATTQWEKRNIALECPS
                     WDPETCIQCGKCVLVCPHAVIRSKVYDPKLLEKAPATFKSTDAKWKDFPGMKYTVQIS
                     VSDCTGCGLCVQNCPAKNKTDASKKAINMVPQVPLRETENENWKFFLSIPDMDREKLN
                     KGTVKDVQLLRPLFEFSGACAGCGETPYVKLISTLYGDRALIANATGCSSIYGGNLPT
                     TPWAVNADGRGPAWSNSLFEDNAEFGMGMRLALDKQSEYAVELLKKLAPEIGTELVEA
                     ILGADQGTEAGIAAQRERVAALKAKLMGIPGLDAKDLLSLADVLVKKSVWIVGGDGWA
                     YDIGYGGLDHVLASGRNVNVLVLDTEVYSNTGGQMSKATPRGAVAKFAAAGKPAGKKD
                     LAMMAMSYGNVYVARVSLGANDAQVLKAFQEAEAYPGTSIIIAYAHCIAHGFDLKMGL
                     EHQKAAVNSGAWILMRYNPALAAEGKNPLILDSKEPSIPLSDYIYSETRYKMLTLSNP
                     AEAAKLLAEAEADVKARWRMYKHLAAMPVE"
     gene            7700..8377
                     /locus_tag="PLY83_02545"
     CDS             7700..8377
                     /locus_tag="PLY83_02545"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOV28947.1"
                     /translation="MEKEPRTNLEDIAYRKIRSAILARGLRPGARLAEPALAGMTGMS
                     RTPVRAALRRLAAEGLVTITPNQGAVVSAPSAKDIRDAYITREPLEALAARLAAETAD
                     ESGILRLKEAAEKEVQTLRARNLSEYIEANSEFHLTVAGMSDNKPLVAAIQSCLALTN
                     VCLAFFDPFYEVQVEESRSNWEHRLLIEALQARDPFWAEAAMRVHIRSSFAYLDIAKI
                     EREMKAQ"
     gene            8374..9639
                     /locus_tag="PLY83_02550"
     CDS             8374..9639
                     /locus_tag="PLY83_02550"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HOV28948.1"
                     /translation="MNLIERREGIGAAVDIGSTSLSARLADLASGEILAERIVPNPTR
                     RWGLDVVRRLAEAANPQKAAEMAQVLRGRIQSLVCELAESAGCSPLETDEIVLVGNPA
                     MHALFWNLPPDSLIRAPFEPADPRPREACCFDLGMPALRKARLTWPALVAGYVGADAL
                     CALDAALETPGIRLPFLLVDTGTNSEVILATRGGILCASAAAGPALEGGNLSCGMAAE
                     KGAIHSFRLEKGRLVPSVIGDSPPKGLCGSGLVDLVALLLEAGGIDASGTLCPESAPK
                     AIRERCERQGKIRTFALFPGFFALSQPDVRALQLAKGAIAAAESILLAEAGIQAADLQ
                     DILMAGAFGNALDPVHAGRIGLVPETGRPARGIGNAALQGAVLYLAAPAAGERRHQNL
                     LERITHVRLHEDSRFQETFMARLSLQPWK"
     assembly_gap    9813..9912
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9913..10391)
                     /locus_tag="PLY83_02555"
     CDS             complement(<9913..10391)
                     /locus_tag="PLY83_02555"
                     /inference="COORDINATES: protein motif:HMM:NF015995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HOV28949.1"
                     /translation="MKRKILALFLVVLFSSVPAWGAGKFAVGSKTFAEQLVLGKMGVA
                     ILKDLGFAVDDKVPLGGTAILRQALVSNQIQGYFEYTGTAATVFFKEKTMFPTPEATY
                     GFVKAADEKNGLAWLARLPLNNTYCLMMRAADAEKHGIRSISDLAALVEKNPRALKLG
                     "
     gene            complement(10433..11062)
                     /locus_tag="PLY83_02560"
     CDS             complement(10433..11062)
                     /locus_tag="PLY83_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOV28950.1"
                     /translation="MYLDTLSHEVILHLALEHLELVFCACLAAFSLALSIGIWVTRPG
                     MKGRTAPRLVAQVLFLGQAVPSLAVIGLVMVVFGTGAPTAIFALTLGALVPMLRNVVA
                     GLRGVPAPVLDAARGNGMPPWTVLVRVELPLALPAIFAGIRTAVVIAIGTAALSSQIG
                     AGGLGSLIFTGLAMFDMPLVLAGAIPTALLAIAADKVLGLAETRFRGKW"
     gene            complement(11044..12216)
                     /locus_tag="PLY83_02565"
     CDS             complement(11044..12216)
                     /locus_tag="PLY83_02565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01186.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HOV28951.1"
                     /translation="MTGVFSGEPHIVLENVGKTFENGAAAVRGLSLKVYRGETLVLIG
                     PSGCGKTTTLRLVNRLVEPTEGRILFDGENTLDMNPDRLRQRMGYVIQGIGLFPHMSV
                     EDNVAVVPRLKKMPRQAVKETVARCLDLVGLPIQAWGKRWPRELSGGQRQRVGVARAL
                     AGDPEVILMDEPFGAVDPLTREVLQDELLALRERLGKTIVFVTHDIHEAMKMGDRVAV
                     MKGGELVQVGEPLALLSRPADNFVADFVGADNHLAMFSFVKVKDVPPRTENLPVVGER
                     ATFGEALDLVERTGIQYGRRRSVYVVDASGRVLGYADLEGIRGKADPVRAALKPAPSV
                     APESSVHDALLRMVENGVINLLVLDENRRMKGLLTFGDLNRLIENQRRGEGCTSIP"
     gene            complement(12213..>12643)
                     /locus_tag="PLY83_02570"
     CDS             complement(12213..>12643)
                     /locus_tag="PLY83_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOV28952.1"
                     /translation="LALAGRGIGAIPAVIALFLYSQLPMLRNTIVGLRNVDPAVVDAA
                     RGMGMNPLRLLFAVRLPLAFPLIMAGIRSSAVMGVGVAAIAAYIGAGGLGTYIMLGIS
                     NSNRELILTGAVSTTLMALALDLCLGAAQKRLERRVGMAP"
     assembly_gap    12644..12743
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12748..13596)
                     /gene="folD"
                     /locus_tag="PLY83_02575"
     CDS             complement(12748..13596)
                     /gene="folD"
                     /locus_tag="PLY83_02575"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOV28953.1"
                     /translation="MTANLLDGKKIASEIRENVARRAEELKARKVVPGLTVILVGEDP
                     ASKVYVGQKEKACREAGFKSDLLRLPETTTEEELLSVIGRLNADPSVHGILVQLPLPS
                     RIDPEAVIAAIDPAKDVDGFHPVNVGHLVSGVPGMVPCTPLGIVRMLESYRIPLPGKH
                     AVIVGRSSIVGKPMAHLLLARDCTVTVCHSRTPDLGYFTRQADVLVAAVGRPRLITAD
                     MVKPGAVVVDVGINRLEGGLVGDVDFEAVREVAGWITPVPGGVGPLTIAMLLENTLSA
                     ALRCSR"
     gene            complement(13633..14964)
                     /locus_tag="PLY83_02580"
     CDS             complement(13633..14964)
                     /locus_tag="PLY83_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOV28954.1"
                     /translation="METGVLISLVLFLGLIVIGMPLGWVFIASTAVGLFLIETPLSFM
                     AGTFFHSLNTPVVMAIGFFVFAGGLISEAGLADRIVRFSYALVGKAKGGLIVVGIVAT
                     LFMSALTGSSVPCISALIPLLVPRLEKFGYQKRYTTAVLCSSSFMGYLIPPSVPALIY
                     CLVAQQSVAALFLATVLPGLLLALGYCILNYFIVDKYTFPSVEKPVLPDNFRDSMKEL
                     GAATWAALPALGCPLIILVGIYGGICTPNEAGAIAVIYCMIVGFFVYRDLKLGNFSKA
                     MVNTCVTLGMIALLLGGGTVLTRFMTREGVAQALAEAMLGLFHSKFMILISINLFLLL
                     LGMFLDGIPILILAVPLILPLANELGINLVHLGSMIIVNIGLGVMTPPFAMSIFVGSR
                     LSGVSAGELIPPMMLYLFCVGIPVLLLTTYIPFLSCWLPTLALGAQVVGPW"
     gene            complement(14966..15493)
                     /locus_tag="PLY83_02585"
     CDS             complement(14966..15493)
                     /locus_tag="PLY83_02585"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HOV28955.1"
                     /translation="MFNFLQQGITAVEKWICAAGLFASTLLTFAQVINRYWLHYEIMW
                     IGDIALYIFVITYILAIALAASQKGHIAVEMLPERIFRDKPVGKQAYAVAMDVLTLTA
                     ILVFLHPAYGFFRQSIRYPEYGTLVRWFNTGWLVQVMFVTIVLSALHMAYHIGNGVFE
                     LRKQLWLKSQQGGSR"
     gene            complement(15641..16720)
                     /locus_tag="PLY83_02590"
     CDS             complement(15641..16720)
                     /locus_tag="PLY83_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOV28956.1"
                     /translation="MKKHGSLFKGIVSVVLLAGIALAFSVSPAAAAPEYKWKFAQTTV
                     RPTQAISMKLFCDLVKKYTNGRMEIKFYPDGLMGSHDEIFHAVQDGSIEIGNFAPYVN
                     LVPGGMFNWMPWTISSWEEAEIAFGQPNGILYKVLEKAYNEVGMHILYTNSQGPYGLG
                     NNVREIRTPADLKNLKFRVSSSLGSAKGLMNMGKGTGMTVETIAWGELYNALSRGVVD
                     GCWDMWPSLIEERHAEVLKYYTALDWMWDGNQIAVNKAKWDALPKDIQAAITRAAREA
                     EAHQYKLAKAAVKDMEARLKKMPNFKIYYPTEKEREMFRQKAQPLSIWEELCKPWLDK
                     AYPGQNMTQKILKELEAIRKQVHKN"
     gene            complement(16837..18432)
                     /locus_tag="PLY83_02595"
     CDS             complement(16837..18432)
                     /locus_tag="PLY83_02595"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HOV28957.1"
                     /translation="MEFDVLIRNGLIVDGTGAKGFRADLGVEGGRIAAIGDLSGTSGK
                     SVLDAGGRIVCPGFIDTHSHSGILALCKPDTPHNVMQGITTCVVGQDAMGAAPIAEPF
                     VGPWKKAMAGLEGSYDLDWSWRSVADYLDRLDSVDLGPNFAYLAPHGNIRMSVMGLDN
                     RRPTSGELEKMKALLEECLKQGAFGMSTGMIYPPCSFAETEEFVELARVLEKNDAVFV
                     THQRSEADAILSSMDEIIEIGKKSGCRIHFSHFKVAGKKNWGLFEAVLGKLDECSRQN
                     IRVSVDQYPYVAGSTTLSVILPPWVHDGGTDRMLERLADPEARKRMTADIEKGIPGWD
                     NFVDIAGVEGIFITFVKTQKNQWAVGKSLAEIGSRVGKDPLEAAYDLLLEEEGTAGLV
                     DFYGLEEHVVRILQRPEQNVCTDGIMGGKPHPRVYGTYPRVLGKYVREGALDLPTAIR
                     KMTGKPAEVLGIKDRGLLKVGLAADIVVFDPEKVRDTADYSDPVRYPEGIEQVLVNGK
                     ILVKDGVHCPVRAGRVLRYTGRG"
     gene            complement(18458..19927)
                     /locus_tag="PLY83_02600"
     CDS             complement(18458..19927)
                     /locus_tag="PLY83_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOV28958.1"
                     /translation="MIDLTVDEAKLERAVRRARERNIVIPTFKQMKDPDKHTPAKIKE
                     RLKKVGLWDVDSANLFRITWKNEPAKTGGLYGGVNYLELPKAITGVDARIVALVGKWF
                     PTGAHKVGASFGCLVPRLVTGQFDPTSQKAVWPSTGNYCRGGAYNAQLLGCESIAILP
                     EGMSKERFDWLKTVAGEVIGTPGTESNVKEIYDKVWELRRTRDNIMVFNQFEEFGNHL
                     WHYEVTGHAMEEVLKSIMGPKDRFAGVTVTSGSAGTTGCGNYLKTVYPNAKLAVGEAL
                     QCPTLIWNGFGAHRIEGIGDKHVPWIHDVKGTDMVMAVDDADCMAMVRLFNEPAGREY
                     LLGQGVPESFVEKLPLMGISGAANVVMAIKMAKYYELTSRDIILTVFTDSMELYGSRL
                     EEMRAERGKPYTVSDAAVDHGRHVLGIGREAMLEMNYYDKLRVHNLKYYTWVEQQGKT
                     SEELNAQWYDYDEYWGSIRSMADAIDEKIEEFNARVGLL"
     gene            complement(19965..21209)
                     /locus_tag="PLY83_02605"
     CDS             complement(19965..21209)
                     /locus_tag="PLY83_02605"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOV28959.1"
                     /translation="MPRPASLNCVLCGRGFEPENAPYTCPDCGLDGTLDVNIDLEAAK
                     KAGFSRESLRQCRDESLWRYRAILPVEKEENIPSLRVGWTPLYESKALASRYGVRAVY
                     VKDDGRNPTASLKDRASAVGVAKALDAGQSAIACASTGNAASSLSGFSAVSGLPCYIF
                     VPERAPEAKVTQLLVYGARVLLVKGDYADAFNLATAAIDRFGWYNRNCAINPYLVEGK
                     KTCALEIAEQLTWEVPDRVFIAVGDGCCIGGLYKGFADLLELGFIDRMPKLVGVQAEG
                     SRPIADAFASGAPRITPGKEDTLADSIAVGAPRNWAKALRAVRKSGGTMVTVTDEEIL
                     SAIRELARTTGVFGEPAGVTGFAGFRKMAESGALSADERVAFVVTGNGLKDAKSARKA
                     VGVPVSVTPDLNELIPLLSRFN"
     gene            complement(21202..22413)
                     /locus_tag="PLY83_02610"
     CDS             complement(21202..22413)
                     /locus_tag="PLY83_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HOV28960.1"
                     /translation="MSLSEARKHRLLEDCRELVRRPALSGQEKNRAEFIESAMKALGY
                     DEVTVDALGNVVGRIVLGSGGPRILLEGHIDQVDVTDPSKWTHDPFGAEIENGRLYGR
                     ATTDMIGNVGAMIHAAAHVKQDFGGLLDGEILVAGSVHEECFEGVACELIAKAFTPDC
                     VVIGEPSNLTLKRGQRGRAEIVVETYGKSAHSSDPSVGLNAVKKMAKLLLAIEERFVP
                     KAHPVLGKGILEVTDVISTPYPGASVIPERCTATFDRRLLVGETKEVVLAQVQEILDE
                     EMAKDPQLKASVRLAVGEDRCYTGAPIKAERFAPAWLFEEDHPLVQAALAGLRSVGQD
                     PVVSHYYFCTNGSYYAGKAGIPTIGYGGSLETLAHVVDEYIELDQLYLACEGYAGIIR
                     SVLAGKGCRRA"
     gene            complement(22410..23633)
                     /locus_tag="PLY83_02615"
     CDS             complement(22410..23633)
                     /locus_tag="PLY83_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HOV28961.1"
                     /translation="MLDGKRQEELSGFCQDLLRSPSLSGAEREVGLRVAEAMRSHGYD
                     EVTVDPCGSVIGQIRFGKPGPVLLLEAQMDHVETGDFTRWSQYPYGAFVRGSRLYGRG
                     ASDQKGCLAAMVMAGAFLRQDLADRLRGSLVVAGTVFQEKFEGVASRGVAMACSPDFV
                     VTGEASGLELVRGQRGRAELLLETAGKMAHSSHPEFGVNAADSMVRLLAALSRSFQPP
                     SDPFLGKGILVLVRLSTEPEEGVGAIPDRARAVFDRRLLAGETKEDVLSQLEEILEKE
                     AAGDPSLQWKLTVASSEDRCYTGIPIRAEHFAPAWVLEEDHPFLEAAKAGLKGMGLDP
                     RISRGPGFGTNGCFYGGQLHLPTIAFGPSFAGLAHAVDEYIELDQLFGACRGYYGIAA
                     SALGGPGIFQEVCEK"
     gene            complement(23728..25080)
                     /locus_tag="PLY83_02620"
     CDS             complement(23728..25080)
                     /locus_tag="PLY83_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HOV28962.1"
                     /translation="MIAFFDWLAGQLWGWPMMILIFACGIVFSFKTGFFQIRHLGYVL
                     KQTLFKIFDRKTKGEGTMTPFQAVSTALAGTVGNGNIAGVATAIAIGGPGAVFWMWVV
                     AIFGMMTKMVEVTLAVHYRVRDEKGNFYGGPMYYIEKGLGKQWKPLAQFFAAMMILGA
                     LGTAVWVQPATMASAMKNVFNIPPLVTAGVSVVLTALVVLGGFKRIGQFCEKLVPFMC
                     AFYIIGALGIIGVNIARLPEVFGLIFRYAFQPAPAVGGFAGASLILAMRRGMSRGMFS
                     NEAGMGSAPMVHATAITDHPVRQGLFGVFEVFMDTIVICTMTAMVILIAAPDLWHSGL
                     NGVDLTFAAFESLYGAFGKAIVAIGVLLFAFSTMVGYTVEYETSVVYLFGSKAVKPFL
                     WLYLIPPFIAVTQPVEFVWTIVDVSTGIEGIPNMIALLLLSGVFMKVYRDFLAKEGLK
                     "
     gene            25219..26133
                     /locus_tag="PLY83_02625"
     CDS             25219..26133
                     /locus_tag="PLY83_02625"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOV28963.1"
                     /translation="MNLNQLRVFCTVVSEGGFLQASRTLYMSQPAISQHVAALEKSVG
                     VRLFNRKGRNVSLTPEGRTLLVLAKEVLEKADGIPERFREMKELKTGKLSVGFEPHPA
                     MSLLPPVLKRFRTEFPDIQVTLLTDRQGSLLQRLASGEIEMAVVGGNRRNLPSTGLIT
                     RSLGMESLTLIAPPDFPRQEADPLPPEALNGKPIARYIPTCFLSPVVEDYLLQHQVKP
                     KSDLWLDDPAVAARFVSEGICLAIVNRGILGGRAACFPTREIPLEGLEAISWEISAVY
                     SSRLGLSYAGWAFEKILESEALRILKSA"
     gene            complement(26201..27463)
                     /gene="glyA"
                     /locus_tag="PLY83_02630"
     CDS             complement(26201..27463)
                     /gene="glyA"
                     /locus_tag="PLY83_02630"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOV28964.1"
                     /translation="MYGKAWEKLKETDPEIAAVIRREARRQQNQIELIASENVVSTAV
                     LAAMGSVLTNKYAEGYPGKRYYGGCEEVDAAEELARERAKKLFGADHANVQPHSGSQA
                     NMAVYFAALKPGDTILAMNLAHGGHLTHGSPVNFSGQLYNIVPYGVRRDTETIDFEQV
                     ERLALEYRPKLIVCGATAYPRVIDAEAFRAIADRVGAFLMFDMAHIAGLVAAKLHKDP
                     VPFCDFVTTTTHKTLRGPRGGMVLCRAEQAKAVDKAIFPGMQGGPLMHVIAAKAVAFQ
                     EALKPDFAQYQRRIVENARVLGEELARRNFRLVSGGTDNHLLLVSLADRGLTGKAMEV
                     ALDRAGITVNKNAIPFDPQPPMVTSGFRVGTPAVTTRGFGPAEMAKIAEWIERVAKDP
                     QDEEQLARIKGEVLELCRAFPLYPDFEA"
     gene            27647..27874
                     /locus_tag="PLY83_02635"
     CDS             27647..27874
                     /locus_tag="PLY83_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV28965.1"
                     /translation="MFLPLEEDGLVDLGRVVALVREGNETAIILRDGSVMATGFTPMT
                     LARRFERFQEATRPGAPLDRPLKKRKGPIRS"
     gene            27871..>29156
                     /locus_tag="PLY83_02640"
     CDS             27871..>29156
                     /locus_tag="PLY83_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV28966.1"
                     /translation="MTPSAKYTAKDIQILEGLEAVRRRPGMYIGDTGPRGLHHLVYEV
                     VDNSIDEAMAGFCTEIEVCLKSDGSVSVTDNGRGIPTDIHPQTGRPAAEVVLTKLHAG
                     GKFDKKAYQVSGGLHGVGVSVVNALSEWLEVTIWRDGTTHRQRFERGIPVTDLEAGEP
                     AERRGTRIHFLPDSTIFSETVFSWDILSGRIRELAFLNPGLCITLKDENEPKEKIFKY
                     DGGIALFAEYLNRGKTVLFAPPLTVRGEKDGVIVEVSIQYNDSYLERIYAFANLIHTV
                     EGGTHVSGFRTALTRAVNEMARRQKLLREKDENLGGDDLKEGLTAVISVKLPEPQFEG
                     QTKTKLGNGEIKGIVDSIVYEGLMELLEDRADVLKPLVENAVKARQAREAARKARDLV
                     RRKSAMSGLELPGKLADCSSKNPAECELYIVEGDSAG"
BASE COUNT         5194 a         9811 c         8901 g         5050 t
ORIGIN      
        1 ctggggctgt ccgtgaccca gaagaattac cccggggctc tctttttcgc ggcgggcgac
       61 taccaccacc acgtcgggac gaacatctgg gacagccgga acgcaccccc gcccccgccg
      121 caggccgcgg gcctgcggcg cttctccctg atcctgcccg cccgggagga cgtggaaagc
      181 ctgaaaaacc ggaccctcca gtccggctat cccatcgaaa ccctgcccgc ggaaggcggc
      241 tttctcctcc gcgacgcgga ccgcatcgcc gtgggcatcg aggccctgcc gtgagcgaat
      301 tcggccggag gctctccctt ctcatcgccg cagccttcgg catcttcgcc tactcgaacg
      361 tctactattt cctggcgaac cttctgggct cccgcggggt ttccccccag gccgccgggg
      421 tggcggtcag tctcttctac gtggcgacca cggtgttccg gccggccggg ggctggctcg
      481 tggaagggtt cgggctgcgc ccgacgctgg tgggcgccgc tctcgttgcc ttcgccggca
      541 gcgtcggaat cgcctttgcc ggatcgaacc tttccctggt tttcgcctgc cggatcctca
      601 tgggcctcgg ctacagcgcc ttcatcgtgg ccttcaccgc ctaccagaac ctgttcgtcc
      661 cgccggaaaa gcgcggcttc gccttcgccc tgatgaccgc gggatccatg gttccccacg
      721 tgtccatcat ccccgcggcg gactggctga tccggcacgg acacgtcgcc gcctacctgt
      781 ccctttctcc cctcatggcg ctgttctgcg ccgccctggg aatcgccttc gggaaggtct
      841 cgctcgcaaa aggagcctcc cgcaaagaag cctggggatc ctaccgggac ctcttctccc
      901 tggccccggt ccgtgccctg ctggcctcca tgttcctgct ctccctgacc gactccatgg
      961 tgctctgcgt cggcgcactc ctggttcccc gggggctcgt tccttcggct ttcctggtgt
     1021 ccagcgccgc tgccgccctg gcggtccgca tcgccaccct gcacttcgtg gaccggatcc
     1081 cccggccccg catcgccgcc ccgagtttcg gcctcatggc cctggccatg gcgatcctga
     1141 gcctgatgcg tagcaacgcc ggagccgcgc tttgcggggc gatgttcggc ctgggagtcg
     1201 gcatgggctt tcccgcccac ctggccatgg tgggggacat gacccccgag cgcctgcgcc
     1261 cgaaagcgac cgccatggtg tggttcgcca tggactccgg ctgggccgtg acccccttcg
     1321 tcttcgggct tctttccccg gtcctgggag actcgctggc gttcgggggg ttcgcggccg
     1381 tcatgctggc gggggccgcc ggggcctgga ggttcctctg gaggccggtt ttagccgagg
     1441 aaaaaagctg agggaaaacg aaaaaggagc gcccggcacc ggtgccgggc gctccttttt
     1501 ttctcttatt cttccgttgc gggattttcc gcggctgccc ctgcccccgc cttcccggcc
     1561 tcgagcctct ccagctcctc ttcccgtgcc accgcccggg cgatcagtcg gttcatgacg
     1621 gcgttcgtga cccggaggct gatccaccag gcggcaaaaa gctcggcagc cgaaacagcg
     1681 aatgccgtca cgctgatctg gccgatgcgc agctccaggc ccggcgcgaa cctccgggtg
     1741 aggacgttga aggcgacaaa aaccccccac aggatcgcca gcagcaggaa ctggcgcatg
     1801 cagcccaggc gggccgccgc tgaacttcgt cttcccctca agcgcaatcc ctctttccgg
     1861 tttcggatgt cctcctatta tggaggtcac gcgcccgccg ttcaactcca ggagtgcagt
     1921 ttggagtaga attccctcgg tgggaagaat ttccggaagg aaggatcacc atggagttgc
     1981 ccgaaaaggg aaacgaagcc gaaaaggaac agtccgtgct ggagagcctc aaggaatggg
     2041 tgtccgggat gctgttcgga tccttcgtcg agatcggcac ccaggcgatc cgcaaggaag
     2101 ccttcgacat caacgacaac ctcatgctgc tgctgttcgg ggactttctc gggattccca
     2161 acccgatctc ctactacacc ctggagcttc tgccctacgt cgccgaggag ctcgtgccct
     2221 gggaaaggcg catgcagaac cgcaagacca tcgtggccga aaaggccggc cagtacgatt
     2281 tcggttaagg aggtccccat gacgcgaccc gcataccgtc ccttcagctt cttcggcggc
     2341 aaggggggaa cgggcaagac gacctgtgcc gccgcccggg ccctggcgct gtccgaggaa
     2401 ggaatcaaga cccttgtcgt ttccaccgac cccgctcact cgctggccga tgccttcgac
     2461 ctttcgatcg gagaggagat cggccagatc gccccgaatc tctgggccat cgagatcgac
     2521 gccgaaaccg aggcccagaa gtacatgaag gggatccagg agaagatgct ccacatcgtc
     2581 agcccggtca tcgtggacga gatcaagcgc cagatcgaga tcgcctacag ctcgccgggg
     2641 gccgaggagg ccgccatctt tgacaagttc atcgagctga tggaacagct gaattccccg
     2701 tacgaggcga tcgtcttcga cacggccccc acgggacaca ccctgcgcct gctgaccctg
     2761 ccggagatcc tcggagcctg gctcgaccac ctcctcgaga agcgcgcccg ggccgtcgag
     2821 ctcatgagca tggtggccaa gtacgaccgg gaagtccggc agaagctgga gtcggatccc
     2881 atcaccgata tcctcgggaa aagaaaggcg aaattcgaaa ccgcccggcg catcctgacc
     2941 gacccggccc tgtgcgcctt ttatttcgtc ctgaacgccg agaagctgcc gatcctagaa
     3001 acggagcggg ccgtccggct gctgacgaag tacgggatcc ccatcggggg gatcctggtc
     3061 aaccgggtga tccccgaagg cgcgggggat ttcctggaga agcaccggct tgcccagcag
     3121 ggctatctcc gccagatccg cgaaacgttc gcgagcctgc ccgtcaccga gatccccttc
     3181 cttgaaacgg atatccgggg aatcaatgaa ctggataaaa ttacaggact cttgaaaaat
     3241 ctttagttta gcatgacaat aaaggcgatc gaccgggagg tgatcgccat gggaggaaac
     3301 atgaacctcc gcagccctgc cttcgacgag ggaggaccga tccctgcagt ttgcggctac
     3361 aagaagggga acgtatcgcc ccccctgcgc tggagcctgt cgcctgccgg aacgcgcagc
     3421 ttcgccctcc tcgtggagga ccccgatgcg cgggggtggg ttcactggat tgcctggaac
     3481 atcccgttct cggcctcgga gctgaaggag ggcgtgcccc ccacgccgca gcttccggac
     3541 ggcacgaggc aggggctgaa cgacttcgga aagatcggct acgggggccc gtgccctcct
     3601 tcgggcgtcc accgctacac cttccgcctg atggccctgg acacggtcct ggacctgccg
     3661 cccgggtccg agcgctatga cctgatgaag gccgtggacg ggcacgtcct ggaggaaacg
     3721 cggttgaccg gaactttctc cgccaactcc tgaccggcga aacgctccgc tttccctgcc
     3781 ccccggcatg acggggggca cttttgcgct cggggttccc ttcgctgccg gcttttcaat
     3841 ccgggttgac atctgtcacc atttttgaaa aattgtgata gcatagagcg gcttggagta
     3901 tatcgtcgac aaaacgcagg atttcatcaa acaacccggt cccttcgggg aaccgattcg
     3961 aaaaacacgg gagcgcggct cccaaaggag gtccggttca atggcaacaa ggccaatgca
     4021 cacgcttgac ggcaacgaag cagcggcgta cgtggcgcac aagaccaacg aggtcatcgc
     4081 catctacccg atcaccccat cctccccgat gggcgagtgg gccgacgaat ggtccgcaaa
     4141 gggaatcaag aacatctggg gaaccgttcc gctcgtttct gaactgcaat ccgagggcgg
     4201 cgcctcgggc gccgtgcacg gggcgctgac cgccggggcc ctcaccacca ccttcacggc
     4261 ctcccagggg ctcttcctga tgatcccgaa cctctacaag atcgccggag agctgacgag
     4321 caccgtcttc cacgtggcgg cccgcaccgt tgccacccac gccctctcca tcttcggcga
     4381 ccacagcgac gtcatggccg tccgcgcttc cggcttcggg ctgcttgcgt ccaactccgt
     4441 gcaggaagtc atggacatgg ccctcatcgc ccaggcctcg accctcgagg cccgcgtgcc
     4501 cttcatccac ttcttcgacg gcttccgcac ctcccacgag gtcagcaagg tggagatgct
     4561 caccgaggac gacatgcggg ccatgatcga cgaggagctc gtgaaggccc accgggcccg
     4621 cggactctcg cccgaccgcc ccgtgatccg cggcaccgcc cagaaccccg acgtcttctt
     4681 ccaggcccgc gaagcctgca acccctacta cgacaaggcc cccgagatcg tccagcgggc
     4741 catggacaag ttcgcctcca tcgtcggccg ccagtaccac ctgttcgact acgtcggcgc
     4801 tccggacgcc gagcgagtcc tggtcatgat gggctccggg gccgagaccg cccacgaaac
     4861 cgtcgactac ctgacggccc gcggcgagaa ggtcgggctt ttgaaggtcc gcctctaccg
     4921 gccgttctcc gtgtcgcact tcctggaaac cctcccgaag agcgtgaaga ccatcgccgt
     4981 cctcgaccgg accaaggaat ccggcgccat cggcgaaccg ctctacctcg acgtggtggc
     5041 cgccctgaag ggccgccccg acgtgaagat cttcggcggc cgttacgggc tctcctccaa
     5101 ggacttcacc gccgccatgg tcaaggccgt tttcgacgag gccgccaagc tcgagccgaa
     5161 gaacggcttc accatcggca tcgacgacga cgtgaccttc cggagcctgc cgtacgaccc
     5221 ctccttctcc acggaggacc cgaagaccgt ccgctgcctg ttctacggcc tgggctccga
     5281 cggcaccgtg ggcgccaaca agaactccat caagatcatc ggcgaggaca cccccaacta
     5341 cgcccagggc tatttcgtct acgactccaa gaaatccggg tccatgacgg tttcgcacct
     5401 gcgcttcggc cccaacccga tccgcgcgat ctacctcatc gaccgggcca acttcgtggc
     5461 ctgccaccag ttcgcgttcc tggagcagta caacgtcctg aagtccgccg agaagggcgg
     5521 caccttcctc ctgaacagcc cctacgggcc cgacgaggtc tgggatcacc tgccgaggac
     5581 gatccagaag gagatcatcg acaagaacct gaagttctac gtcatcgacg ccgtcgacgt
     5641 ggcgcagaag gccggcatgg gcggccgcat caacaccgtc atgcagacct gcttcttcgc
     5701 catcagcggc gtcctcccca aggacgaggc catcgcggcc atcaagcaca gcatcgagaa
     5761 gacctacggc aagcgcggcc ccgagatcgt gaagaagaac ttcgaggccg tggactcctc
     5821 cctcgcccac ctgcacgagg tgaaggtgcc ggggaccgtc accagcacgt tcgacatccg
     5881 gccgccggtt cccgccgagg cccccgagtt cgtccagaag gtcaccgcga agatcatcgc
     5941 cggagagggc gacagcctcc ccgtgagcgc cctgcccgcg gacggcacct tcccgaccgc
     6001 cacgacccag tgggagaagc gcaacatcgc cctggaatgc ccctcctggg accccgagac
     6061 ctgcatccag tgcggcaagt gcgtgctggt ctgcccccat gccgtcatcc gctccaaggt
     6121 ctacgatccc aagctcctgg agaaggctcc ggccaccttc aagtccaccg acgcgaagtg
     6181 gaaggacttc cccggcatga agtacaccgt ccagatctcc gtctccgact gcaccggctg
     6241 cggcctgtgc gtccagaact gcccggcgaa gaacaagacg gacgcgtcta agaaggccat
     6301 caacatggtt ccgcaggtcc cgctccgcga gaccgagaac gagaactgga agttcttcct
     6361 ctccatcccc gacatggatc gcgagaagct gaacaagggg acggtgaagg acgtccagct
     6421 gctgaggccc ctgttcgagt tctccggggc ctgcgcgggc tgcggtgaaa ccccctacgt
     6481 gaagctgatc agcacccttt acggggatcg ggccctcatc gccaacgcca ccggctgctc
     6541 ctccatctac ggcgggaacc tgccgaccac cccgtgggcc gtcaacgccg acgggagggg
     6601 gccggcctgg tccaactccc tcttcgagga taacgccgag ttcggcatgg gcatgcgcct
     6661 ggccctggac aagcagtccg agtacgccgt ggagctcctg aagaagctgg ctcccgagat
     6721 cgggaccgag ctggtggagg caatccttgg ggccgaccag ggaaccgaag ccgggatcgc
     6781 cgcccagcgc gagcgggtgg cggccctgaa ggccaagctc atgggcatcc cgggactcga
     6841 tgccaaggac ctgctcagcc tcgcggacgt cctcgtgaag aagtccgtct ggatcgtcgg
     6901 cggagacggg tgggcctatg acatcggcta cggcgggctc gaccacgtcc tggcttccgg
     6961 caggaacgtg aacgtcctcg ttctcgacac cgaggtctac tccaacacgg gcggccagat
     7021 gtccaaggcc accccgcgcg gcgccgtcgc caagttcgcg gcggccggca agccggccgg
     7081 caagaaggac ctcgccatga tggccatgag ctacgggaac gtctacgtgg cccgggtctc
     7141 cctcggggcc aacgacgccc aggttctcaa ggccttccag gaagccgagg cctacccggg
     7201 cacctcgatc atcatcgcct acgcccactg catcgcccac gggttcgacc tgaagatggg
     7261 gctggaacac cagaaggccg ccgtcaactc cggcgcctgg atcctgatgc gctacaaccc
     7321 ggcccttgcg gcggagggca agaacccgct catcctcgac agcaaggagc cttccatccc
     7381 gctttcggac tacatctaca gcgaaacccg ttacaagatg ctgactttga gcaaccccgc
     7441 ggaggccgcg aagctcctgg ccgaggcgga ggccgacgtc aaggccaggt ggcgcatgta
     7501 caagcacctc gccgccatgc ccgtcgagta ggacgcgcag cacgggggaa gcccaaccgg
     7561 aagcaaccga agggcccggg atcttcccgg gccctttcct tttttcgaac ccggggccga
     7621 ctcgtgagag aatagggaac gggaaacgaa atccgtgccg tcctttcgct cagcggcctt
     7681 cggaagggag agccgtccat tggaaaaaga acctcggacc aacctggaag acatcgctta
     7741 ccggaaaatc cggagcgcga tcctggctcg cggcctcagg ccgggtgccc ggctcgccga
     7801 accggccctg gcgggaatga cgggaatgag ccggacgccg gttcgcgccg ccttgcggcg
     7861 gctcgcggcg gaagggctgg tgacgatcac cccgaaccag ggagccgtgg tttccgctcc
     7921 ttccgcgaag gacatccggg acgcctacat cacgagagag cccctggaag ccctggcagc
     7981 caggctggcc gcggaaaccg ccgacgagag cgggatcctt cgcctgaagg aagccgccga
     8041 aaaagaggtg cagaccctgc gggcccggaa cctttccgag tacatcgagg ccaacagcga
     8101 gtttcatctc accgtggccg ggatgtcgga caacaagccc ctcgtggcgg cgatccaaag
     8161 ctgcctggcg ctgacgaacg tctgcctcgc gtttttcgac ccgttctacg aggtccaggt
     8221 ggaggaatcc cggtcgaact gggaacaccg cctgctcatc gaggctctcc aggccaggga
     8281 tcccttctgg gcggaggcgg cgatgcgggt ccacatccgc tcttccttcg cctaccttga
     8341 catcgcgaag atcgagcggg agatgaaagc ccaatgaatc tcatcgagcg cagggaaggg
     8401 atcggggccg ccgtcgacat cgggagcacc agcctttcgg cccggctggc ggacctcgcc
     8461 tcgggggaga tcctggcgga aaggatcgtc ccgaacccga caagaaggtg gggcctggac
     8521 gtggtgcgaa ggctggcaga agccgccaat ccccagaagg cggccgagat ggcgcaggtc
     8581 ctccggggca gaatccagtc gctggtttgc gaactggccg aatcggcagg gtgctccccc
     8641 ctggaaaccg acgaaatcgt tctggtgggg aacccggcca tgcatgccct gttctggaac
     8701 cttccgcccg actcgctcat ccgcgcgccg ttcgagccgg cggaccctcg cccccgtgaa
     8761 gcctgctgct ttgacctggg gatgccggcc cttcgaaaag ccaggctgac ctggccggcc
     8821 ctggttgcag gctacgtcgg ggccgacgcc ctgtgcgcgc tggacgccgc gctcgaaacc
     8881 ccggggatcc gcctcccgtt ccttttggtc gacacgggga caaacagcga ggtcatcctg
     8941 gccacccggg gagggatcct ctgcgcttcc gccgcggccg gcccggccct ggagggggga
     9001 aacctctcct gcgggatggc cgcggagaag ggggccattc actccttccg cctggaaaag
     9061 gggcggctcg tcccctccgt gatcggcgat tccccgccga aaggcctctg cggaagcggg
     9121 ctcgtcgacc tggtcgcgct gctgctcgag gcgggaggca tcgacgcctc ggggaccctt
     9181 tgccccgaaa gcgctccgaa ggccatccgc gaacgctgcg aaagacaggg aaaaatccgg
     9241 acctttgccc tttttcccgg ctttttcgcc ctttcccagc cggacgtccg ggccctccag
     9301 ctggcaaagg gggccatcgc cgccgcggaa tcgatcctcc tcgcggaggc gggaatccag
     9361 gcggccgacc tccaggatat cctcatggcc ggggcgttcg ggaacgccct cgacccggtc
     9421 catgccggac gcatcgggct ggtcccggaa accggacgcc ctgcccgggg catcggcaac
     9481 gccgcgctgc agggcgccgt cctctacctg gccgcaccgg cggcggggga aaggcgtcac
     9541 cagaacctcc tggaacggat cacccacgtc cgcctccacg aggattcccg gttccaggaa
     9601 accttcatgg ccaggctctc cctgcagccc tggaaatgaa aaagggccga aagcctccct
     9661 gcggcaggga gccattcggc ccggaagcca ccttcccgtc ctaccggatg atcccgtggg
     9721 ttttcaggaa ggcctccgcc acggtagccg gatcctgttt cttctcggcg acctggaagt
     9781 tcagctcggt gatggcttcg tcatccagca ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9901 nnnnnnnnnn nnccgagctt gagggcgcgc gggttctttt ccaccagggc cgccaggtcc
     9961 gagatcgaac ggatcccgtg cttttcggca tcggcggccc gcatcatgag gcagtaggtg
    10021 ttgttcagcg gaaggcgggc aagccaggcc aggccgttct tctcgtccgc cgccttgacg
    10081 aagccgtagg tcgcctccgg tgtcgggaac atcgtctttt ccttgaagaa cacggtggcc
    10141 gccgtccccg tgtactcgaa atacccctgg atctggttgg agaccagggc ctgccgaaga
    10201 atggccgtcc cgccgagggg aaccttgtcg tccaccgcaa atcccaggtc cttcaggatc
    10261 gccacgccca tcttcccgag aaccagctgc tcggcgaacg tcttgcttcc caccgcgaac
    10321 ttcccggctc cccaggcggg aaccgacgaa aacaggacaa ccaggaacaa cgcgaggatt
    10381 tttctcttca tcggggcacg ctcctttctt tttcccgccg gtgacaacgg ggctaccact
    10441 tgccgcggaa acgggtctcc gcgagcccga ggaccttgtc cgccgcgatc gcgagcaggg
    10501 ccgtggggat cgcccccgcc agcacgaggg gcatgtcgaa catcgccagg ccggtgaaaa
    10561 tgaggctccc caggcctccc gccccgatct gggagctgag ggctgccgtg ccgatggcga
    10621 tgacgacggc ggtccggatc cccgcgaaga tcgccggcag ggccaggggc agctccaccc
    10681 tcaccagcac cgtccaggga ggcatcccgt tcccgcgggc cgcgtcaaga accggggccg
    10741 ggacccccct caggcccgcg acgacgttcc ggagcatcgg caccagggcc cccagcgtga
    10801 gggcgaagat cgccgtcggc gccccggtcc cgaagacgac catgacgagg ccgatgacgg
    10861 ccaggctggg aacggcctgc cccaggaaaa gcacctgggc gaccagcctc ggggccgtcc
    10921 tgcccttcat gcccgggcgg gtgacccaga tcccgatcga aagcgccagg gaaaaggcgg
    10981 ccaggcaggc gcagaacacg agttccaggt gttccagggc caggtggagg atcacctcgt
    11041 ggctcagggt atcgaggtac atccttcgcc cctcctctgg ttctcgatga gccggttcag
    11101 gtcgccgaaa gtcaaaagcc ctttcatccg tcggttttcg tcgagcacga ggagattgat
    11161 gaccccgttt tcgaccatgc gaagcagggc gtcgtgaacg ctgctttccg gggccaccga
    11221 gggagcaggt ttcagggccg ccctcaccgg gtcggctttg ccgcggatcc cctcgaggtc
    11281 cgcgtagccc agcaccctgc ccgatgcatc gacgacgtag acggaccgcc tccggccgta
    11341 ctggatcccc gttctctcca cgaggtcgag cgcttccccg aacgtcgccc gctcgccgac
    11401 cacggggagg ttttcggtcc ggggcggaac gtccttgacc ttcacgaagg agaacatcgc
    11461 gaggtggttg tcggctccga cgaagtcggc gacgaagttg tccgccggcc gggacaaaag
    11521 agccaggggt tctccgacct ggaccagctc gccgcctttc atgacggcaa cgcggtcgcc
    11581 cattttcatc gcctcgtgga tgtcgtgggt gacgaaaacg atcgttttcc ccagcctctc
    11641 ccggagggcc agcagttcgt cctggaggac ttcccgggtg agaggatcca cggccccgaa
    11701 gggttcgtcc atcaggatga cttccgggtc ccccgcgagc gcccgggcaa cccccaccct
    11761 ctggcgctgt ccccccgaaa gctcccgcgg ccaccgtttc ccccaggcct ggatcggcag
    11821 gcccaccagg tccaggcagc gggccaccgt ttccttcacg gcctggcggg gcatcttctt
    11881 cagcctcggc accaccgcca cgttgtcctc gacgctcatg tgcggaaaga ggccgatccc
    11941 ctggatgacg taacccatcc gctgccgcag gcggtccggg ttcatgtcca gcgtgttctc
    12001 gccgtcgaag aggatgcggc cttccgtggg ttcgaccagg cggttgacca ggcgcagggt
    12061 ggtcgtcttc ccgcagcccg agggaccgat caggaccagc gtctcccccc ggtacacctt
    12121 caggctgagc cccctgacgg cggcggcacc gttctcgaac gtcttcccca cgttttccag
    12181 cacgatgtgg ggttcccctg aaaagacgcc ggtcatgggg ccattcccac ccttcgttcc
    12241 aggcgtttct gggccgcgcc gaggcagaga tccagggcca gggccatcag ggtcgtggat
    12301 acggccccgg tgaggatcaa ttcgcggttg ctgttggaaa tgcccagcat gatgtaggtc
    12361 ccaaggccgc ccgcgccgat gtaggccgcg atggccgcca cccccacccc catgaccgcc
    12421 gaggaacgga ttcccgccat gatgagcgga aaagcgagcg gcagccggac cgcgaagagc
    12481 aggcgcagcg ggttcatccc catgccccgc gcggcgtcga cgacggcggg gtcgacgttt
    12541 cgaagcccca cgatcgtgtt ccgcagcatc ggaagctggg agtagaggaa cagggcgatc
    12601 accgccggaa tcgccccgat tccgcgcccc gccagcgcca ggannnnnnn nnnnnnnnnn
    12661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12721 nnnnnnnnnn nnnnnnnnnn nnncgggtca gcggctgcac cggagagctg cactgagggt
    12781 gttctccagg agcatggcga tggtgagcgg gcccaccccg ccgggaacgg gggtgatcca
    12841 gccggcgacc tcgcggacgg cttcgaagtc cacgtccccg acaaggccgc cctccaggcg
    12901 gttgatcccc acgtccacca ccaccgcgcc gggcttgacc atgtcggccg tgatcagcct
    12961 cggcctgccg acggcggcca ccaggacgtc ggcctgccgg gtgaagtacc cgaggtccgg
    13021 ggttcggctg tggcagacgg tgacggtgca gtccctcgcg agcagcaggt gggccatggg
    13081 cttgcccacg atggagcttc tgccgacgat gaccgcgtgc ttcccaggca gggggatccg
    13141 gtaggattcc agcatccgga cgatcccgag gggcgtgcag ggcaccatgc cggggacccc
    13201 cgaaacgagg tgcccgacgt tcacggggtg gaacccgtcc acgtccttgg ctggatcgat
    13261 ggcggcgatc accgcctccg ggtcgatccg ggacggcagc ggcagctgga ccaggatccc
    13321 gtgaaccgag ggatccgcgt tcagccggcc gatgacggaa agcagttcct cctccgtcgt
    13381 ggtttcgggc agtctcagaa ggtccgactt gaacccggct tcccggcagg ccttttcctt
    13441 ctgccccacg tagaccttcg aggcgggatc ctcccccacg aggatgaccg tcaggcccgg
    13501 cacgactttc cgggctttca gctcctccgc cctcctggcc acgttctcgc ggatttccga
    13561 ggcgattttt ttcccgtcca gcagattcgc cgtcatgttc tcacctcttt tcagccgttc
    13621 atccgttcaa gcttaccacg gtccgacgac ctgggccccc agggcgaggg tgggaagcca
    13681 gcaggaaagg aaggggatgt aggtcgtcag aagaagcacg gggatcccca cgcagaacag
    13741 gtacagcatc atcggcggga tcagttcccc ggcggagaca ccggaaagcc gggaaccgac
    13801 gaagatcgac atggcaaaag gaggcgtcat gacccccagg ccgatgttca cgatgatcat
    13861 ggatcccagg tgaaccaggt tgatccccag ctcgttcgcc aggggcagga tgagcggcac
    13921 cgccaggatg aggatgggaa tgccgtcgag gaacattccc aaaagaagca ggaacaggtt
    13981 gatcgagatc aggatcatga acttgctgtg gaacagcccg agcatcgctt cggccagggc
    14041 ctgggcgacc ccttcccggg tcatgaaacg cgtcaggacg gtccccccgc cgagcagcag
    14101 cgcgatcatg ccaagggtca cgcaggtgtt gaccatggct ttcgagaagt tgccgagctt
    14161 caggtcgcga tagacgaaga agccgacgat catgcagtag atgaccgcga tggcccccgc
    14221 ttcgttgggg gtgcagatgc ccccgtaaat ccccacgagg atgatcagcg gacagcccag
    14281 ggctgggagg gccgcccagg tggcggctcc gagttccttc atcgagtccc tgaagttgtc
    14341 cggtagaacc ggcttctcga cgctcgggaa cgtgtacttg tcgacgatga agtaattcag
    14401 gatgcagtac ccgagggcca aaagcagccc cgggaggacc gtggccaaaa agagcgccgc
    14461 gacggactgc tgggccacca ggcagtagat cagggccgga acgctcggcg gaatgagata
    14521 gcccatgaag ctggaggagc agagcacggc cgtggtgtag cgcttctggt agccgaattt
    14581 ctccaggcgg ggcaccagaa gcgggatgag cgcggagatg caggggaccg aggaaccggt
    14641 cagggcgctc atgaagagcg tcgcgacgat gcccacgacg atgagtcctc ccttggcctt
    14701 tcccaccagg gcataggaaa accggacgat ccggtcggcc agccccgcct cggaaatgag
    14761 ccccccggcg aagacgaaaa agccgatggc catgaccacg ggggtgttca gcgagtggaa
    14821 gaacgttccc gccatgaagg aaagcggcgt ttcgatcagg aaaagcccca cggccgtgct
    14881 ggcgatgaac acccagccca ggggcatccc gatgacgatg aggccgagga aaagaaccag
    14941 tgaaatcaga accccggttt ccatgctacc ggctcccccc ttgctgagac ttgagccaga
    15001 gctgtttcct cagctcgaag accccgtttc cgatgtggta ggccatgtgg agggcggaaa
    15061 gcacgatcgt gacgaacatg acctggacca gccacccggt gttgaaccag cggaccaggg
    15121 tcccgtactc ggggtagcgg atcgactgtc ggaagaaccc gtaggcgggg tggagaaata
    15181 ccaggatggc cgtcagggtg aggacgtcca tcgccaccgc gtaggcctgc ttcccgacgg
    15241 gcttgtcccg gaaaatccgt tccggcagca tctccacggc gatgtgcccc ttctgcgacg
    15301 ccgccagggc gatggccagg atgtaggtga tgacgaagat gtacagggct atgtccccga
    15361 tccacatgat ctcgtaatgg agccagtagc ggttgatgac ctgcgcgaac gtcaggaggg
    15421 tcgacgcgaa aagccccgcc gcgcagatcc acttctcgac cgcggtgatc ccctgctgca
    15481 gaaaattgaa cacgagcctc acgctcctta agaacgttca aagacaaagg gccatccctt
    15541 tgcccggaat ctatcgcaaa tcgccggacg gacccggcgt ttctttccgc gtggggggca
    15601 aggcccccgg agaggggcct tgcctttcct gcttttccag ctagttcttg tgaacctgct
    15661 tgcggatcgc ttcgagttcc ttcaggatct tctgggtcat gttctggccg gggtaggcct
    15721 tgtcaagcca gggtttgcag agctcctccc agatggagag aggctgggcc ttctggcgga
    15781 acatttcccg ctccttttcc gtcgggtagt agatcttgaa gttgggcatc ttcttcagcc
    15841 gggcttccat gtccttcacc gcggccttcg ccagcttgta ctggtgggct tcagcctccc
    15901 tggcggcgcg ggtgatggcc gcctggatat ccttcggcag ggcatcccat ttggccttgt
    15961 tgacggcgat ctggttgccg tcccacatcc agtcgagtgc cgtgtagtac ttcaggacct
    16021 cggcgtgccg ttcctcgatg agagagggcc acatgtccca gcatccgtcg acaacgcctc
    16081 ggctcagggc gttgtacagc tcgccccagg cgatcgtctc gaccgtcatg cccgtcccct
    16141 tgcccatgtt catgaggccc ttcgcgctgc ccagcgagga ggaaacgcgg aacttcaggt
    16201 tcttgaggtc agccggggtg cggatctcgc gcacgttgtt tcccaggccg tacgggccct
    16261 ggctgttcgt gtagaggatg tgcatgccga cctcgttgta ggctttctcc aggaccttgt
    16321 aaaggatccc gttgggctgg ccgaaggcga tctcggcctc ttcccaggac gaaatggtcc
    16381 agggcatcca gttgaacatg ccgccgggaa cgaggttcac gtacggggca aagttcccga
    16441 tctcgatgct gccgtcctgg acggcatgga agatttcatc gtggcttccc atgaggccgt
    16501 ccgggtagaa cttgatctcc atgcggccgt tcgtgtactt cttgacgagg tcgcagaaga
    16561 gcttcatgga aatggcctgg gtgggccgga ccgtggtctg ggcgaacttc cacttgtact
    16621 ccggcgcggc tgccgcaggc gagacggaaa aggccagggc gattcccgcc agcaggacga
    16681 cactgacgat tcccttgaac aggctaccgt gcttcttcat cgagcaatcc tccctttcgg
    16741 tttgatggaa cgaaggaacc cctttgaaac gtttaacgaa cccaaatccc gggaaacaga
    16801 cggaagacgc accccccttc gcgaacgttt tcctgcctat cctcgaccgg tgtaccgaag
    16861 gacccttccg gcccggacag ggcagtgcac cccgtccttc acgaggatct tcccgttgac
    16921 gagcacctgc tcgatcccct cggggtaacg gaccgggtcg gagtaatccg ccgtgtctcg
    16981 caccttttcc gggtcgaaga ccacgatgtc cgccgcaaga ccgaccttca gcaggccccg
    17041 gtccttgatc ccgaggacct cggcaggctt gccggtcatc ttgcggatgg ccgtcggcag
    17101 gtccagcgct ccctcgcgga cgtacttccc gagaacgcgg ggataggttc cgtacaccct
    17161 cggatggggc ttcccgccca tgatcccgtc cgtgcagacg ttctgctcgg ggcgctgcag
    17221 gatccggacc acgtgctcct ccaggccata gaaatccacc aggcccgcgg tcccctcttc
    17281 ctccagcagg agatcgtaag cggcttccag cgggtctttc cccaccctgg acccgatttc
    17341 cgcgaggctt ttccccacgg cccactggtt cttctgcgtc ttcacaaaag tgatgaaaat
    17401 cccctcgacg ccggcgatgt cgacgaaatt atcccagccc gggatcccct tttcgatgtc
    17461 ggccgtcatc cgctttcgcg cctcggggtc ggccaggcgc tccagcatcc ggtccgttcc
    17521 gccgtcgtga acccagggcg gcaggatgac gctcagcgtc gtgctgcccg ccacgtacgg
    17581 gtactggtcc acggaaaccc ggatgttctg tctcgagcac tcgtccagtt tcccgaggac
    17641 ggcttcgaac aggccccagt tcttcttccc ggcaaccttg aaatgagaga agtggatccg
    17701 gcagccgctc tttttcccga tctcgatgat ctcgtccatc gaggaaagga tcgcatcggc
    17761 ctcgctccgc tggtgggtga cgaaaaccgc gtcgttcttc tcgaggaccc tggccagctc
    17821 gacgaattcc tccgtttccg cgaagctgca gggcgggtag atcatccccg tggacatgcc
    17881 gaaagccccc tgcttgaggc attcctcgag cagggccttc atcttttcca gttcccccga
    17941 cgtgggcctg cggttatcca ggcccatgac gctcatgcgg atgttcccgt ggggagccag
    18001 gtaggcgaag ttggggccca ggtccacgga atccaggcgg tccaggtaat ccgccacgga
    18061 ccgccagctc cagtccaggt cgtagcttcc ttccaggccg gccatcgcct tcttccaggg
    18121 acccacaaac ggctcggcga tgggggcggc gcccatggcg tcctgcccga ccacgcaggt
    18181 cgtgattccc tgcatcacgt tgtgaggcgt gtcgggcttg cagagggcca ggatccccga
    18241 gtggctgtgg gtgtcgataa accccgggca gacgatcctc ccgccggcgt cgagcacgga
    18301 tttccccgat gtcccggaga ggtcgccgat ggcggcgatc cggccgcctt cgaccccgag
    18361 gtccgccctg aacccttttg cgcccgtccc gtcaacgatc agcccgtttc ggatcagcac
    18421 gtcgaattcc attgccttct cctcccagcc ggggagacta gagcagtccc accctggcgt
    18481 tgaactcctc gatcttttcg tcgatggcgt ccgccatgga acggatggat ccccagtact
    18541 cgtcgtagtc gtaccactgg gcgttcagct cctcggaggt cttgccctgc tgctccaccc
    18601 aggtgtagta cttcaggttg tggacccgga gcttgtcgta gtagttcatc tccagcatcg
    18661 cttcccggcc gatgcccagg acgtgccggc cgtggtcgac cgccgcgtcg gaaaccgtgt
    18721 agggcttgcc ccgctcggcc cgcatctcct ccaggcgcga gccgtacagt tccatggagt
    18781 ccgtgaacac cgtcaggatg atgtccctgg aggtcagctc gtagtacttg gccatcttga
    18841 tcgccatgac gacgtttgcc gccccggaga tccccatgag gggaagcttt tccacgaagg
    18901 attccggcac tccctggccg agcaggtact cccggccggc gggctcgttg aacagccgga
    18961 ccatcgccat gcagtcggcg tcgtcgacgg ccatgaccat gtcggtcccc ttcacgtcgt
    19021 ggatccaggg aacgtgcttg tctccgatgc cctcgatgcg gtgggcgccg aacccgttcc
    19081 agatgagggt cgggcactgc agggcttccc cgacggccag cttggcgttc ggatagacgg
    19141 tcttcaggta gttgccgcag ccggtcgttc ccgcggagcc cgaggtgacc gtgacgcccg
    19201 cgaagcggtc cttcggcccc atgatcgatt tcaggacctc ttccatggcg tggccggtga
    19261 cctcgtagtg ccacaggtgg tttccgaact cctcgaactg gttgaagacc atgatgttgt
    19321 cccgggtcct gcgaagctcc cagactttgt cgtaaatttc cttgacgttg ctttccgtcc
    19381 cgggagtccc gatgacttcg ccagcaaccg ttttcagcca gtcgaagcgt tccttgctca
    19441 tcccctcggg gaggatggcg atggattcgc atcccagaag ctgggcgttg taggccccgc
    19501 cccggcaata gttcccggtg gaaggccaga cggccttctg ggacgtcgga tcgaactgcc
    19561 ccgtcaccag gcgcggaacg aggcacccga agctggcccc gaccttgtgc gctccagtgg
    19621 ggaaccactt gcccaccagg gccacgatcc gtgcgtccac ccccgtgatc gccttcggga
    19681 gctccaggta gttgaccccg ccgtagagcc ccccggtttt cgcgggttca ttcttccagg
    19741 tgatccggaa aaggttcgcg gaatcgacat cccacagccc gacctttttc aggcgttcct
    19801 tgatctttgc cggggtgtgc ttatccgggt ccttcatctg cttgaacgtg gggatcacga
    19861 tgttgcgttc ccgtgctctc cgcacggccc tttccagttt cgcctcgtcc accgtcaagt
    19921 caatcatttt tttctcctcc tgtcgtgcgc ctgccctgtc ccggtcagtt gaaccgggac
    19981 aggagcggga tcagttcgtt gagatccggg gtcaccgaaa ccggcacccc gacggccttt
    20041 cgggcgcttt tcgcgtcttt caggccgttg ccggtcacca cgaaggcgac ccgttcgtcc
    20101 gccgaaagcg cacccgattc ggccattttc ctgaaacccg caaagccggt cactcccgcg
    20161 ggttccccga aaacaccggt ggtccgggcc agttcccgga tcgcggagag gatttcctcg
    20221 tccgtgacgg tcaccatggt cccgccggac ttccggaccg cccggagggc cttcgcccag
    20281 tttcgcgggg cccccacggc gatgctgtcc gccagggtgt cctctttccc cggagtgatg
    20341 cgcggtgccc cggacgcgaa ggcgtcggcg atgggtcggc tgccctcggc ctggaccccc
    20401 accagcttcg gcatccggtc gatgaacccg agctccagca ggtcggcaaa ccccttgtac
    20461 agccccccga tgcagcagcc gtctcccacg gcgatgaaca cccggtcggg cacctcccag
    20521 gtgagctgct cggcgatctc cagggcgcag gtcttcttgc cttccaccag gtaggggttg
    20581 atggcgcagt tgcggttgta ccagccgaag cggtcgatcg ccgccgtggc caggttgaag
    20641 gcgtccgcgt aatccccctt caccaggagg acacgggcgc cgtagaccag aagctgcgtg
    20701 accttggcct cgggggctcg ctccgggacg aagatgtagc agggaaggcc tgacacggcg
    20761 gaaaacccgg aaagagagct ggcggcgttc cccgtggagg cgcaggcgat cgcggactgc
    20821 cccgcatcca gcgccttcgc cactcccacc gcgctggccc ggtccttcag ggaagcggtg
    20881 gggtttcgcc cgtcgtcctt gacgtagacg gccctgaccc cgtagcggga ggcgagcgcc
    20941 ttgctctcgt aaagaggcgt ccagcccacc ctcagggagg ggatgttttc ctccttctcc
    21001 accgggagga tggcccggta ccgccaaagg ctctcgtccc ggcactgcct gagggactcg
    21061 cggctgaacc ccgctttttt cgcggcctcc aggtcgatgt tcacgtccag cgtcccgtcc
    21121 aggccgcagt ccggacaggt gtagggagcg ttttccggct cgaatccgcg cccgcagagg
    21181 acgcagttca gggaagcggg cctaggcacg gcggcagccc tttccggcca ggactgagcg
    21241 gatgatcccc gcgtagccct cgcaggccaa gtacagctgg tccagctcga tgtactcgtc
    21301 caccacgtgg gcgagggttt ccagggatcc gccgtagccg atggtgggaa tcccggcctt
    21361 tcccgcgtag tagctgccgt tggtgcagaa atagtagtgg gaaaccaccg gatcctggcc
    21421 gacggaacgg agcccggcca gggccgcctg caccagcgga tggtcttcct cgaaaagcca
    21481 ggccggggcg aagcgctcgg ccttgatcgg cgcccccgtg taacagcggt cctcccccac
    21541 cgcaagccgg accgaagcct tgagctgggg atccttcgcc atttcctcgt ccaggatctc
    21601 ctgcacttgc gccagcacga cctccttcgt ctcccccacc aggaggcgcc ggtcgaaggt
    21661 ggccgtgcac cgctccggga tgacgctggc gccggggtac ggcgtcgaga tgacgtcggt
    21721 aacctccagg atgcccttgc ccaggaccgg gtgcgccttc ggcacgaacc gctcctcgat
    21781 ggccagcagg agtttcgcca ttttcttcac ggcattgagc cccaccgacg ggtccgagga
    21841 gtgggcgctc ttgccgtagg tttccaccac gatctccgcc cggccgcgct ggccccgctt
    21901 cagggtcagg ttcgagggct cgccgatgac gacgcagtcc ggggtgaatg cttttgcgat
    21961 gagctcgcag gcaactccct cgaagcactc ttcatggacg ctgccggcaa caaggatctc
    22021 cccgtccagc aggccgccga aatcctgctt cacgtgggcc gccgcgtgga tcatggcgcc
    22081 cacgttcccg atcatgtcgg tcgtggcgcg cccgtaaaga cgcccgttct cgatctcggc
    22141 accgaacgga tcgtgggtcc acttcgaagg atccgtgacg tccacctggt cgatatgccc
    22201 ttcgagaagg atccggggac cgccgctgcc cagcacgatg cggcccacca cgttgcccag
    22261 ggcgtccacg gtcacctcgt cgtagccgag ggccttcatg gccgactcga tgaactcggc
    22321 ccggttcttt tcctgccccg aaagcgcagg gcggcggacc aattcccggc agtcctcgag
    22381 aagccgatgc ttccttgcct cgctcaagct cacttttcac acacctcctg aaagattccc
    22441 ggaccgccca gcgcggaggc cgcgatgccg taatagccgc ggcacgcccc gaaaagctgg
    22501 tccagctcga tgtattcgtc cacggcgtga gccagccccg cgaaggacgg cccgaaggca
    22561 atcgtcggca ggtgcagctg gcccccgtag aaacacccgt tcgtcccgaa accgggcccc
    22621 ctggaaatcc gcgggtccaa ccccatcccc ttcaggcccg ccttcgcggc ctccaggaag
    22681 gggtggtcct cctccagcac ccaggccggg gcgaaatgct cggcccggat gggaatcccc
    22741 gtgtagcagc ggtcctccga ggaagcgacc gtaagcttcc actgcaggga aggatcgccg
    22801 gcggcctctt tctccaggat ttcttccagc tgggacagca cgtcctcttt cgtctcgccc
    22861 gccaaaagcc gccggtcgaa caccgccctg gcccggtccg gaatcgcccc gacgccttct
    22921 tccggctcgg tcgaaaggcg gacgaggacc aggattccct tgccgagaaa gggatccgag
    22981 ggcggctgaa acgagcggga tagggccgcc agcaggcgga ccatcgaatc ggcggcgttg
    23041 accccgaact cgggatggga ggaatgggcc atcttccccg ctgtttccag cagcagttcg
    23101 gccctgcccc gctgccccct caccagctcc aggcccgagg cttccccggt gaccacgaaa
    23161 tccggcgagc aggccatggc gaccccgcgg gaagccaccc cctcgaactt ctcctggaag
    23221 acggtcccgg ccaccaccag gcttccgcgg agccgatcgg ccaggtcctg ccggagaaag
    23281 gccccggcca tcaccatggc ggcgaggcat cccttctgat cggacgcacc ccgtccgtag
    23341 agacggcttc cccggacaaa tgctccgtac gggtactggc tccagcgggt gaagtcgccc
    23401 gtttccacgt ggtccatctg cgcttccagg aggaggaccg gtcccggctt accgaagcgg
    23461 atttgcccga tcacgcttcc gcaggggtcc acggtcacct cgtcgtaccc atgggagcgc
    23521 atggcctcgg ccacccgcag gccgacctcc cgttcggccc cggaaaggct ggggcttcgg
    23581 agcaggtcct ggcaaaaccc tgaaagctct tcctgtcgtt tcccgtccag catcgagatc
    23641 cctcccggga acgtcccttc cagaaacggc acgggccccg gcgttcagcc agggcctccg
    23701 acctgccgaa accgcggggg aagggtccta tttcagccct tccttcgcga ggaaatcgcg
    23761 gtagaccttc atgaaaactc cgctcagcag cagcagggcg atcatgttcg ggatcccctc
    23821 gatccccgtg gaaacgtcca cgatcgtcca gacgaactcg accggctgcg tgacggcgat
    23881 gaacggcggg atcaggtaca gccacaaaaa cggcttgacc gccttcgatc cgaagaggta
    23941 gaccaccgag gtttcgtact ccacggtgta gccgaccatg gtggagaaag cgaacagcag
    24001 gaccccgatc gcgacgatcg cctttccgaa ggccccgtag agggactcga aggccgcgaa
    24061 cgtgaggtcg acgccgttca gcccgctgtg ccacaggtcc ggggccgcga tgaggatgac
    24121 catggcggtc atggtgcaga tgacgatcgt gtccatgaag acctcgaaca cgccgaagag
    24181 cccctgccga acgggatggt cggtgatggc cgtggcgtgg accatcgggg ccgaccccat
    24241 gccggcctcg ttggagaaca tgcccctcga cattccccgg cgcatcgcca ggatgaggga
    24301 agcgcccgcg aatcctccga cggccggggc cggctggaag gcatagcgga agatcaggcc
    24361 gaacacctcc ggcagccggg cgatgttgac gccgatgatt cccagcgccc cgatgatgta
    24421 gaaggcgcac atgaagggaa ccagcttctc gcagaactga ccgatccgct tgaagccgcc
    24481 caggaccacc agggcggtca aaaccaccga gacccctgcc gtcacgagcg ggggaatgtt
    24541 gaaaacgttc ttcatggccg aggccatcgt cgccggctgg acccagaccg ccgtcccgag
    24601 agccccgagg atcatcatcg ccgcgaagaa ctgcgccagg ggcttccact gcttgccgag
    24661 ccccttctcg atgtagtaca tcgggccgcc gtagaagttg cccttctcgt ccctgacgcg
    24721 gtagtggacg gccagggtga cctcgaccat cttcgtcatc atgccgaaga tcgccaccac
    24781 ccacatccag aacacggctc ccggcccgcc gatggcgatt gccgtggcga ctcccgcgat
    24841 gttgccgttg ccgaccgttc ccgccagcgc ggtcgagacg gcctggaagg gcgtcatggt
    24901 cccctcgcct ttcgtcttcc ggtcgaaaat cttgaaaagc gtctgtttca ggacataccc
    24961 gaggtgccgg atctggaaaa agccggtttt gaagctgaaa acgatgccgc aggcgaagat
    25021 caggatcatc atgggccatc cccagagctg ccccgccagc cagtcaaaga acgcgatcat
    25081 ctccgtccct cccggaaaag aaagataggc cttcacctga aatgaaggtt gttttctgag
    25141 gatacgtcct gagttataat tttcaatacc ttaatatatc gttatcttta taagcaggac
    25201 ttatagggag gccttgcaat gaacctgaac cagctgaggg ttttctgcac cgtcgtctca
    25261 gaggggggat tcctccaggc ttcgcgaacc ctctacatgt cgcagccggc catcagccag
    25321 cacgtggctg ccctggaaaa gtccgtcggg gtccggcttt tcaaccggaa ggggcggaac
    25381 gtttcgctga cgccggaagg acggaccctc cttgtcctgg cgaaggaggt cctggaaaag
    25441 gcggacggga tcccggagcg tttccgcgaa atgaaggaac tgaagacggg caagctttcc
    25501 gttggtttcg agcctcaccc ggccatgagc ctgctgcctc cggtcctgaa gcgcttccgg
    25561 accgagttcc cggatatcca ggtgaccctt ctgaccgacc ggcagggttc tctccttcag
    25621 cgtcttgcgt ccggggagat cgaaatggcc gtcgtcgggg gaaaccgcag gaacctgccc
    25681 agcacggggc tcatcacccg ttcgctgggc atggaaagcc tgaccctgat cgccccgccg
    25741 gatttcccgc ggcaggaagc cgaccctctt cccccggaag ccctgaacgg aaaacccatc
    25801 gcccggtaca tccccacctg cttcctctca cccgtggtgg aggactacct ccttcagcac
    25861 caggtgaagc cgaaatccga cctgtggctg gacgaccccg ccgtggccgc ccgcttcgtt
    25921 tcggagggaa tctgcctggc catcgtgaac cgcgggatcc tgggaggaag ggctgcctgc
    25981 tttccgaccc gggagatccc cctggagggg ctcgaagcga tcagctggga aatcagcgcg
    26041 gtctattcga gccgcctggg actttcctac gccgggtggg ccttcgagaa gatcctggaa
    26101 tccgaggcgc tgcgaatcct gaaatcggca tgaaaaaggg cgggatcccc gggaggtccc
    26161 ccgccctgga caaatgccgg ccatttctct cgacgccgcc tcaggcctcg aaatccgggt
    26221 aaagcgggaa cgcccggcac agttccagca cttccccctt gatccgcgcc agttgctctt
    26281 catcctgcgg gtccttcgcg acgcgctcga tccattccgc gattttcgcc atctctgccg
    26341 ggccgaagcc tctcgtggtc acggcgggag tcccgacccg gaatccgctc gtcaccatgg
    26401 gaggctgagg gtcaaagggg atggcgttct tgttcacggt gatccccgcc ctgtcgaggg
    26461 caacctccat cgccttgccg gtcagccctc gatccgccag gctcacgagg agaagatggt
    26521 tgtcggttcc ccccgaaacc agcctgaaat tgcgccttgc cagttcctct cccagcacgc
    26581 gcgcgttttc cacgatcctg cgctggtact gggcaaagtc gggcttcagg gcttcctgga
    26641 aggccacggc cttggcggcg atgacatgca tcaagggccc tccctgcatc ccggggaaga
    26701 tcgccttgtc gaccgccttc gcctgctcgg cccggcacag gaccatgccg ccccggggtc
    26761 cccggagggt cttgtgggtc gtggtggtga cgaagtcgca gaacggcacc gggtccttgt
    26821 ggagcttcgc ggccaccagg cccgcgatgt gggccatgtc gaacatcagg aaggccccca
    26881 ccctgtcggc gatcgcccgg aacgcttccg cgtcgatcac cctcggatac gccgtcgcgc
    26941 cgcagacgat gagcttcggc cggtactcca gggccaggcg ctccacctgt tcgaaatcga
    27001 tcgtctcggt atcgcggcgc accccgtagg gaacgatgtt gtacagctgg ccggagaagt
    27061 tgaccgggga accgtgggtc aggtgtcccc cgtgggccag gttcatggcc aggatggtgt
    27121 cgcccggctt cagggccgca aagtagaccg ccatgttggc ctgggaaccc gagtggggct
    27181 ggacgttggc gtgatccgcc ccgaaaagct tcttcgccct ctcccgggcc agctcctccg
    27241 ccgcgtccac ctcctcgcac cctccgtagt agcgctttcc agggtatcct tccgcgtact
    27301 tgttcgtcag gacggaaccc atcgccgcca gcaccgccgt ggaaaccacg ttttcggaag
    27361 cgatcagctc gatctggttc tgctgccggc gggcctcccg ccggatcacc gcggcaattt
    27421 cggggtccgt ttctttcagt ttttcccaag cctttccgta catgggcatc accctccctg
    27481 gctctgatgg ggtcgcttta aattttccca caaacgatgt gtttagcaaa atagataaaa
    27541 cattcaacac gaagccccgc gaaatgtcaa cacccggcct tcaaaccggc ttttggtata
    27601 attgctttgt aggcagacca tcttttcccg cctggaggcc aatcctttgt tcctgccgct
    27661 tgaagaggac ggactcgtcg atctgggccg cgtcgtcgcc ctcgtgaggg agggcaacga
    27721 aacggccatt atcctgcgcg acggcagcgt catggccacg gggttcaccc ccatgaccct
    27781 ggcccgccgc ttcgagcgtt tccaggaggc cacccggccg ggagcccctc ttgaccgccc
    27841 cttgaagaaa aggaaaggac cgatccgttc atgacgccat ccgccaagta caccgcaaaa
    27901 gacatccaga tcctggaagg actcgaggcc gtccgccgcc gtcccggcat gtacatcggg
    27961 gacaccggcc cccggggcct ccaccacctc gtctacgagg tcgtggacaa ctccatcgac
    28021 gaggccatgg ccggcttctg caccgagatc gaggtctgcc tgaagtccga cggcagcgtc
    28081 tccgtgacgg acaacgggcg cggaatcccg acggacattc acccgcagac cgggcgcccg
    28141 gccgccgagg tcgtgctgac caagctccac gccgggggca agttcgacaa gaaggcctac
    28201 caggtttccg gcggccttca cggcgtcggc gtttccgtcg tgaacgccct gtccgagtgg
    28261 ctcgaggtga cgatctggag ggacggcacg acccaccgcc agcgtttcga gcgcggcatc
    28321 cccgtgacgg acctggaagc gggagaaccg gccgagcgaa gggggacgcg gatccacttc
    28381 cttcccgact ccacgatctt cagtgaaacc gtcttctcct gggacatcct gtccggccgc
    28441 atccgcgagc tggccttcct gaacccgggc ctctgcatca ccctgaagga cgagaacgag
    28501 cccaaggaga agatcttcaa gtacgacggg ggcatcgccc tgttcgcgga atacctgaac
    28561 cggggcaaaa ccgtcctttt cgccccgccc ctgacggtcc ggggggaaaa ggacggggtc
    28621 atcgtcgagg tctcgatcca gtacaacgat tcctacctgg aacggatcta cgccttcgcc
    28681 aacctcatcc acacggtgga aggcgggacc cacgtcagcg ggttccggac cgccctcacc
    28741 cgggccgtca acgaaatggc ccgccggcag aagctcctca gggaaaagga cgaaaacctc
    28801 ggcggagacg acctgaagga aggactgacc gccgtcattt ccgtcaagct gccggaaccc
    28861 cagttcgagg gtcagacgaa gaccaagctg ggcaacggcg aaatcaaggg catcgtcgat
    28921 tccatcgtct acgagggcct gatggagctc ctcgaggacc gtgcggacgt cctcaagccc
    28981 ctggtggaga acgccgtgaa ggcgcgccag gccagggaag ccgcccggaa agcccgggac
    29041 ctggtccgcc gaaagtccgc catgtcgggc ctcgagcttc cgggcaagct ggcggactgc
    29101 tccagcaaga accccgcgga atgcgagctg tacatcgtgg agggcgacag cgccgg
//
LOCUS       DAOOVN010000013        26740 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_1220_length_26740_cov_13.960615, whole genome shotgun
            sequence.
ACCESSION   DAOOVN010000013 DAOOVN010000000
VERSION     DAOOVN010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 26740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26740
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1220_length_26740_cov_13.960615"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..1075
                     /locus_tag="PLY83_02645"
     CDS             44..1075
                     /locus_tag="PLY83_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOV28967.1"
                     /translation="MPLIPVRTVGITFNLKTGHGEEEEEYDPLETILALKSEIERYGF
                     DVLLFNQDDLFLESLSKNRPDFVLNIAEGRGNTRSREAQVPCILESLGIPYSGSDALA
                     LGMTLDKYTTGQFLRARGVAVPGQFVVNRLDRLEDPMLEALFLENRRYIVKPRWEGSS
                     KGVFADSVVANLSQLKERARYILKRYDEPALVEEFLPNDEVTVAVAGNQRPRTLGIMR
                     ILPRGEVGGVFLYSIERKRQWRECMRYENGDALPLEVRRTIEAEALKAYEALELRDLA
                     RIDLRLDWDGMPRVIDVNPLPGLNPEFGDLPILFELNGGDYPSLIRILLEEAFSRCGL
                     LWAESCRHA"
     gene            1068..2054
                     /locus_tag="PLY83_02650"
     CDS             1068..2054
                     /locus_tag="PLY83_02650"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOV28968.1"
                     /translation="MPDPGRVLIAVGLERTDRPDVLDTLRCVESVKAVFAGRGLPVEP
                     LYVRSEDFPGLDRVRETILSGSPLKIFNLFEGFSGDSGKEVTFASMLEKLRVPFTGNP
                     SVALEMCLDKAKAKQRLARRGILVPRGVFLQRLEGAPWKGLNFPLFVKPCFEDGSVGV
                     EKDSLIQDAEGIEALLRMKLAGFPRGILLEEFIPGKEFSVGFLGAPPYEVLGVSMIDY
                     SLYSGSLPFLHYGSKWDPQCTEFHEILPVLEEGIAPSLRERLVDTARKAGEALGCRGY
                     FRVDLREREGRLYVLDVNPNPDLNEDSGFARQGRKRGYSYPEVVERILFYAN"
     gene            2044..2547
                     /locus_tag="PLY83_02655"
     CDS             2044..2547
                     /locus_tag="PLY83_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOV28969.1"
                     /translation="MPIDRNDSLPGGSLEEYLAAAEGTGAFRPSELDVLQEVLTDAFA
                     DPSRGYLFLEERLPGGQLAGFAVFGRAPMTDWAWDLYWIVVERSLQGRGIGRRMLEKL
                     EAIALAVTGKVILRVETSGRKEYEKQRQFYLAAGFRETGRIRDFYEEGDDLVTYCKFL
                     PGEKSLR"
     gene            complement(2508..3734)
                     /locus_tag="PLY83_02660"
     CDS             complement(2508..3734)
                     /locus_tag="PLY83_02660"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOV28970.1"
                     /translation="MDLQDFFNMKARRIGPSIIAQMATMLGPDGISFTSGEPSADLIP
                     LSGLSEAFSRAAGQASLFSYQNTAGYLPLREWIAEWMSREALAPAWLDPSCVILTNGS
                     QGALNLLAETFIDPGDLILTESPTYPEALLAFQREGARIIPVPVDEDGPIPEALEERM
                     AGVKAKFFYTVVNFQNPSGCTTSDARKAAILELARRRGFFILEDDPYRYLRFEGKPTK
                     SYLEMAGEDRRVVYLGSFSKIIAPGVRCGWCAFPLSLKEKLLEIRLSLELSPPALTQA
                     AVFEFLRVCDLGGHLEKLAREYKKRRDSLVEALNREASQLGLKLSVPKGGFFLWGNCE
                     GVADMLEFARYAALEKKVGFIPGRFFFPVEGEGADSIRFAFAKAAPEQSREGAARFAD
                     ALESYRRLFSPGRNLQ"
     gene            3870..5408
                     /gene="malQ"
                     /locus_tag="PLY83_02665"
     CDS             3870..5408
                     /gene="malQ"
                     /locus_tag="PLY83_02665"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF011080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOV28971.1"
                     /translation="MILKGKRACGILHHLTSLPSAAGIGDLGAGARAFLDFLAEAGQS
                     LWQMLPIGPVDRGLGGSPYCGVSAFAGNELLVDLNELEEIGLLPPGEGTPLWTRIDAP
                     IDWALVFQRKGSALDRAWERFRVGRADLREEFHAFCRKQENWLKDYSLFCAIKASQGG
                     KPWNEWPPDLRLRSPKALKEAAGTLEQERERVEFGQWLFFRQWEKFRCEAHRRGIVLF
                     GDLPIYVGLDSADVWCWQEGFDLGEDGQPRTVAGVPPDYFSPTGQRWGNPTYRWEVHR
                     KDGFAWWRSRVTHALSLFDCIRIDHFRGLSAYWAIPAGEETALRGKWIEAPGEELLEA
                     LLSDHPNLPIVAEDLGIITPDVERLKNRFGLPGMRVLQFGFLGDTGTNPHAPHNFPEN
                     AVAYTGTHDNNTARGWFEEELEEEGKTVLSRYLGHPVTAERVADDLVRLAYSSPAAWA
                     ICPLQDGLGLDGRARMNQPGRKTGNWTWRCRIPEPDVARKLAETGVLFGRARSSRSNV
                     LNEP"
     gene            5507..5767
                     /locus_tag="PLY83_02670"
     CDS             5507..5767
                     /locus_tag="PLY83_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HOV28972.1"
                     /translation="MEVLKVSATSQPKSVAGAMAAVLREKGVVEVQAVGAGAVNQAVK
                     SIAIARGYVAPNGIDMVCIPAFAKIDIDGEERTAIKFQLEAR"
     gene            5914..7191
                     /gene="hisS"
                     /locus_tag="PLY83_02675"
     CDS             5914..7191
                     /gene="hisS"
                     /locus_tag="PLY83_02675"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOV28973.1"
                     /translation="MESIKAPRGVRDILPDESWKWAFIFGVTRKIAEDFGYREVHLPI
                     FEQTELFSRGIGETTDVVEKEMYTFSDRSGRSLTLRPEATASMVRAYLEHGMASGPQP
                     SKLWCAGPMMRYERPQKGRYRQFWQLDFEAIGSASPLVDVEVIALSLEIFRRLGLSNL
                     EVLVNSVGCPSCRPVYRSALQGYLRERFDELCPTCQGRFDRNPLRILDCKVETCRAIT
                     DGAPDVRDHLCGECRSHFDEVLQGLDLIGAKFRIDKRLVRGLDYYTKTAYEVLSGDLG
                     AQNAVCGGGRYDNLAEAIGGPHTPGVGFAAGLDRIVLTMEQQGLSFGREPSISVIVVV
                     ADEAARDEAVRLLYDLRRAGVPSDMDTSGKGVKAQMKAAGAAKASWVCILGSSEVARG
                     VVSLKEMATGSQEEIEREKVVEQLAAKTKAAWC"
     gene            7215..9020
                     /gene="aspS"
                     /locus_tag="PLY83_02680"
     CDS             7215..9020
                     /gene="aspS"
                     /locus_tag="PLY83_02680"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOV28974.1"
                     /translation="MALSDRYFDDSWKKTVYCGRVGPQDEGRQTVLNGWIRKRRDLGG
                     IIFLELWDYTGTVQVVFNPEDTPEAHELAKDLRSEYVVAVKGKVRRRPAGTENPNLPT
                     GEWELLAEDLLVLSPASPLPFEMSEVDRVDENLRLKHRYLDLRRPKMQDNLRIRARAA
                     KFTRDYLVGHGFLEVETPILTKSTPEGARDYLVPSRVNQGEFYALPQSPQLFKQILMV
                     SGCDRYFQIAKCFRDEDLRADRQPEFTQVDLEMSFITETDIIGLLEGYVKGLFSEILS
                     EEIKTPFLQLTWREAMEAYGSDKPDLRNPLKICDLDPVFSGGTFEPFRKILSEGGAVR
                     GLWVPGGASLSRKEMADLEERAKGLGAQGLAAFQIKSGELKGPLVKSLDEAGRQKLLE
                     RSGLSDGDALFVAAGEWASTCSMLGTLRLEIASKLDLVDRSSWAFLWVRDFPLLEWDD
                     EEQRWCAMHHPFTAPHPEDVALLEKDPGRVRSRAYDLVLNGSEIGGGSIRIHDPAMQE
                     RVFQVLSFTQEQARERFGFLLEALSYGTPPHGGLAIGFDRLVALLCGCRSIREVMAFP
                     KTAKAQCLMSGAPSEVESKQLRELGICISRGEGQA"
     gene            9313..9996
                     /locus_tag="PLY83_02685"
     CDS             9313..9996
                     /locus_tag="PLY83_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOV28975.1"
                     /translation="MSMIRFLGHSAFHVSAPGLEGLIDPFLRENDQAVVQPGDFNSLT
                     HLFVTHGHWDHIGDTIELGLKTGATVVANAELCRYFSGKGLKTRPMYIGGWVPFPFGR
                     VKFVPALHGSGIMEGDQTLYGGVAVGIVLEVGKFRIYHAGDTGLTAEMGLLEALKIDL
                     ALLPIGGCYVMDIPDALRAVEMIRPRRVVPMHFNTFPGIEANPDGFLEALPEWTVGIR
                     LDPGQSLQF"
     gene            10027..10878
                     /locus_tag="PLY83_02690"
     CDS             10027..10878
                     /locus_tag="PLY83_02690"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HOV28976.1"
                     /translation="MRGRLKGNPFFLAFLFGVAFLNAFSGTASAGEIKLTSDTLIYDP
                     ASRLVRAEGNVRLERQGLDLKANFAEGDPAGERFRAWGAVRAHWEEQKMDLSAEEVSL
                     FDRPPRRILASGQVLLERPRERLAASSVEWVFGPSPEYVAKGGVRVEVPGRVLEAAEA
                     GRKNDRFWARQVTRFEDQGEKISLKAGQIEAATEGDSLKDLTATGQVSAVVVGSDGVP
                     VKLKGDRLLYSKGKNTVALVGNAEAVQSGRSVRSESILYNLATRRIEAQGRPQLIFTP
                     GKETASP"
     gene            10875..11606
                     /gene="lptB"
                     /locus_tag="PLY83_02695"
     CDS             10875..11606
                     /gene="lptB"
                     /locus_tag="PLY83_02695"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOV28977.1"
                     /translation="MTSSRVLRAVELQKSYKGRQVVGSVSIEVRQGEIVGLLGPNGAG
                     KTTTFYMIVGLVRPDAGEILIDDREITTLPMYKRARMGVGYLQQEASVFRNLTVRENL
                     ELVLQELEKTPAERARMIDHLVAEFGIDRILDTPGYALSGGERRRVELARCLAAMPEF
                     LLLDEPFSGIDPIAVFDIQQMILGLRGKGYGILLTDHNVRDTLAITDRAAILHQGRIM
                     VEGTPREVSQSEVARKYYLGDRFTL"
     gene            complement(11601..12062)
                     /locus_tag="PLY83_02700"
     CDS             complement(11601..12062)
                     /locus_tag="PLY83_02700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOV28978.1"
                     /translation="MGNSRTTVWCLGNPLRGDDGVGVRCGELLLETPCPHLEIVLCET
                     VPENFSAPLRRNPPDFLVIIDACAMGTAPGTIRLLDPDCLDGLTETTHGLPLGQILRE
                     ALPRERIAVIGIEPKETGFSLDLSPEVEAAAREVVRCIREGSWRRFTPNPS"
     gene            12104..13663
                     /gene="murJ"
                     /locus_tag="PLY83_02705"
     CDS             12104..13663
                     /gene="murJ"
                     /locus_tag="PLY83_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOV28979.1"
                     /translation="MSSPLSKMVRHAMRMMVGTFASRILGLLREIITAALFGATRQLD
                     AFFVAYTLSNLSRQLLAEGALSAAFVPVFSRVVVEEGREKAAKLARQVTTVLLLAGST
                     AVTVGIFAAPILVRVMAPGFSGADTQLAVLLTRLMFPFLLLVSVAALAMGVLNSLDSF
                     FIPAVAPALSNLLYIVLLAILAPRMGIWALPTAVLCGGVAQMLLQWWRSGALGVPLLP
                     ARPDWNNPELKRVIRLFFPYMAGLSLNQVNPVISRMVGSFLEGGTISVLSYADRIIQL
                     PLGLFVIAISQAVLPMLSRIGAEDTAGFAQMTRDALRFTLFVILPVVVGMSLLARPMV
                     HLIFVRGAFNEWAWEATSRALALYALGLPGMACSTVVMRGLYARRLPREALKVTGFTV
                     FLNLVFCVTLVRFFAYAGLALATSLAFSGGALMGAWTLRRHLETPIGVLTGEWLLRHG
                     VPLALMAAGILVGARLFPYDPAFRLWGKLFWVLGAVSAGAVLYAVASWMSGCAEWKWV
                     SGAFGKEERVH"
     gene            13660..14574
                     /locus_tag="PLY83_02710"
     CDS             13660..14574
                     /locus_tag="PLY83_02710"
                     /inference="COORDINATES: protein motif:HMM:NF013589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HOV28980.1"
                     /translation="MREEGRNAGGLPVAVVSRGLLAGGRFRIFAVKADRLVDETMRRQ
                     NLSPLAAAALGRLMMAGMMMALDIKLSKARVTLKLDGGGPLGLVVADAEPQGRVRGYV
                     EHPEVDLPPSSSGKLDVASGVGKNGTLVVFRDVRMKEPVSSQVPLVSGEIAEDIAAYY
                     RFSEQTGTAVGLGVLARPGQVISAGGFMVQVLPGSEDSQVSIVEERVRNTPSVSRLLE
                     EHPDARSAMEALFRELDIEWFEDVEFSLSCRCSRDLAKALLLESLGKEIPGERQEVRC
                     HFCGTVYTFSKEELERDWPPEAGGANEE"
     gene            14593..15132
                     /locus_tag="PLY83_02715"
     CDS             14593..15132
                     /locus_tag="PLY83_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV28981.1"
                     /translation="MKKALWMCVFLFIAAVGGVAWGGQAKSLDDLLAERSANVWVDGQ
                     VLGDLVLGAKARLTFIYVDPLLASAARNDPGAPEWLTWHSRHLGEKNLKGRALFLLHF
                     ETFTPWELDPGRIHVGNYTVSSEDVLSMREYSLTGELPSGFTGTLPFSVPKSFLKGGT
                     QIVLAYGEEDSAQWKVPGK"
     gene            15299..16330
                     /gene="tsaD"
                     /locus_tag="PLY83_02720"
     CDS             15299..16330
                     /gene="tsaD"
                     /locus_tag="PLY83_02720"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOV28982.1"
                     /translation="MRGALTSRRFLTLGIESSCDDTAVAVLEGPTTVRSSRISSQLEL
                     HAPYGGVVPELASRDHQERILPLVFSALSEAGVTDPGTELALIGVTMGPGLMGSLMVG
                     VMTAKALAQAWKVPLLGVNHLEGHLFANVIQNPGLKPPFLTLLVSGGHTEVILARAFG
                     DYAVLGCTRDDAAGEAYDKLAKVLGLGYPGGPAVDRLAAKGNPDAFPLPVPLAETSRV
                     EFSFSGLKTASLNLIRQLEREGPLPVEDICASFQKAVVEALLKKVELAVQLTGVRRIA
                     VSGGVAANSGLRRAFSGRKDWEVFIPPAILCTDNGVMVAAAAYHGYRRGKRTGLDFSP
                     DPALELLSD"
     gene            16511..16798
                     /gene="groES"
                     /locus_tag="PLY83_02725"
     CDS             16511..16798
                     /gene="groES"
                     /locus_tag="PLY83_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOV28983.1"
                     /translation="MHLKPLADRVVVKVLTLEEKTRGGIVLPDTAKEKPQEAKVMAVG
                     SGKVLENGQKLAPEVKVGDRVLFGKYSGTEVKVDGEEYLILSERDILAIVE"
     gene            16834..18459
                     /gene="groL"
                     /locus_tag="PLY83_02730"
     CDS             16834..18459
                     /gene="groL"
                     /locus_tag="PLY83_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806319.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOV28984.1"
                     /translation="MAKILNFGEDARRSLERGINKVADTVGVTLGPKGRNVVLEKKFG
                     SPTITNDGVTIAKEIELEDPFENMGAQLLKEVASKTNDVAGDGTTTATVLARAMIREG
                     MKNVAAGANGILMRRGIEGAVDVVVDELKALSIAVKERSKIAQVAAISANDKVIGNLI
                     AEAMEKVGEDGVITVEDGQTVGTTLETVEGLQFDKGYISPYMVTNAERMEAVLEDAYI
                     LINDGKINNVKDLLPVLEKVVQTGKPLMIIAEDVEGEALATLVVNKLRGILQVVAVKA
                     PGFGDRRKAMLQDIAIVTGGQVVSEEVGLKLENADLSMMGRAKTVRVTKEETTIVEGK
                     GDPEKIRARAAQIRKELEDSTSEYDKEKLQERLAKLVGGVAVIQVGAATETEQKELKH
                     RIEDALNATRAAVEEGIVAGGGVALVNSLQALDKHIETLSGDERTGATIVRKALTEPL
                     HLIASNAGYQGDVVVEKVLGLPKGHGLNAATGEYLDMVKEGIIDPLKVTRSAVQNAAS
                     IAAMVLTTEALVGDKPEKKESMPSMPGGMGDYD"
     gene            18610..20151
                     /gene="guaA"
                     /locus_tag="PLY83_02735"
     CDS             18610..20151
                     /gene="guaA"
                     /locus_tag="PLY83_02735"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOV28985.1"
                     /translation="MAPHSNIVILDCGSQYTQLIARRIRELSVHSEILPWDSGAEEIL
                     SHNPNGIVISGGPRSVLEEGAPVIPEAVLHSGIPILGICYGMQLLAHLLGGTVRQAPL
                     AEYGPAEVRILDSRTPLLKGLPETLSVWMSHWDSVISLPSGFSVIAESSGGLIAGFSD
                     ETGRISGLQFHPEVAHTPQGMEILRNFLFSVCGCVADWNLSDWAEQAIRAIREQVGSD
                     RVICGLSGGVDSTVAAVLTARAIGDQLECIFVNNGLLRLNEAEEVLEAYREMKLKVHY
                     VDATRRFLEGLKGVSEPERKRKIIGELFVRVFEEKAGEIGGASWLLQGTLYPDIIESG
                     HKGKGAAVIKTHHNVGGLPERMSLKVLEPLKDLFKDEGREIGRMIGVPEAILKRHPFP
                     GPGLAVRCLGEITAERLETLRKADAIFIEEIRKAGLYDRLWQAFCVLLPARSVGVMGD
                     VRTYAEAVALRAVESLDAMTADWARLPADLLNRAANRICNEVPGVNRVVYDVTSKPPA
                     TIEWE"
     gene            20182..20949
                     /gene="larB"
                     /locus_tag="PLY83_02740"
     CDS             20182..20949
                     /gene="larB"
                     /locus_tag="PLY83_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HOV28986.1"
                     /translation="MSRTSKDAFRDFLEQVRCGRLSVDEGLEVLSSMATLHLEEIRLD
                     SHRSFRCGIGEVIYCPGKTRLQLEKIAEACRERPETLIFSRMEREQAEFVRERLQGFC
                     YDEVARTGYRKGDPAVERGCVAVISGGAADQRVAAEAALVAELCGCEVARYFDVGVAG
                     LHRLLSVLPELRKADVVIAVAGMEAALPTVAAGLLNTVVIAVPTSAGYGANLGGAAAL
                     LGVLNSCCPNVLAVNIDNGLGAGISASLIAGGGSRRG"
     gene            20942..22063
                     /locus_tag="PLY83_02745"
     CDS             20942..22063
                     /locus_tag="PLY83_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOV28987.1"
                     /translation="MVEGPSAETWKNKPRGTFVPFVVFFAALSTIAFLLAFPLLRPLA
                     WSIILSFFSYPLFQFLHDRLFRGRLANLSAGLTTAVIIAMLMLPAILFGLYLAHDGMA
                     LYGRLTTIIEKLEKQDLSLILNLIPPSVREFLAPWLSQVNLFGDLFGQLGRWFSGWIA
                     GVSRGLFESALRLVFQMIVITVSSFFMIRDGHVIVEYIRDIVPLSAEETDAMISRGRR
                     MLQAVVYGFTFTAAVQGFLGALGWWWVGLPNALIFGVLMFIFAIIPFVGTALVWVPGA
                     LYLFATGQHGAALTLAFWGLAVVSTVDNFLRPIFISEGSKVHILIVFVGVVGGLATLG
                     LLGIFLGPLVMTLFFFCLDAYRRIWMSLRQAAQPEEPRR"
     gene            22060..22683
                     /locus_tag="PLY83_02750"
     CDS             22060..22683
                     /locus_tag="PLY83_02750"
                     /inference="COORDINATES: protein motif:HMM:NF015181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HOV28988.1"
                     /translation="MIPVLAVSGFKKSGKTTLCRELAACFQEVGVRVGFVKRTHEPSV
                     RCGGTDTGLLLEAGVPIVLWAPDGLQAENREEQPELDWMVERFMPGVDLVLLEGGKSL
                     SLPRVWVGNPEECPAEVKGILAWFGAFPPAGRIPSFRPGQEKDLAAYLLDRLLGKRQS
                     PSVSLHVDGRKIPLKPYLERFLGQAILGMIRSLKGTQGRDISIHIRN"
     gene            22841..24802
                     /locus_tag="PLY83_02755"
     CDS             22841..24802
                     /locus_tag="PLY83_02755"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOV28989.1"
                     /translation="MGTQVILLVALAGVLALVYAVWATGRINAVKVDNERVNDLSGII
                     HGGALAFLYREYKALVPFVVIVGGLLFWKIGTGSALCFVLGAFCSAMTGYFGMNVATK
                     ANGKTAFSAMKGMNQALTVAFTGGSVMGMNVVGIGILGILLCTVIFKDPNIIVGFGFG
                     ASSIALFARVGGGIYTKAADVGADLVGKVEAGIPEDDPRNPAVIADNVGDNVGDIAGM
                     GADLFESYVNAIVAAMAVGIVTFGNAGMVYPLLLAALGILSAILGTFFVKVGENGDPQ
                     AALSRGTYATGAFLIVGTFFLTRAMFGELTLFYAVVAGTIAGVAIGFATEVYTSSSYK
                     HVKEIAEASLTGPATNILSGLGIGMKSTAVPVLLICAATLVGVRFGGLYGIACSAVGM
                     LAITGMVLSVDAYGPIADNAGGIAEMSHLPHEVRQITDKLDAVGNTTAAVGKGLAIGS
                     AALTALALFVAYAQAAHIDVIDLRDPKVMVGLFIGGLLPFIFAALSIGAVGRAAQHMI
                     DEVRRQFREIPGIMEGTGRPEYERCVDISTAAALKEMVVPGLMAVIAPVLVGLALGAG
                     ALGGLLGGSIVTGVMLAIFMANSGGAWDNAKKYIEEGHHGGKRTPAHAAAVVGDTVGD
                     PFKDTAGPSMNILIKLMSVVAVVIAPLFS"
     gene            24920..26659
                     /gene="dnaG"
                     /locus_tag="PLY83_02760"
     CDS             24920..26659
                     /gene="dnaG"
                     /locus_tag="PLY83_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOV28990.1"
                     /translation="MGQDEAVREIKERLDIVELVGDAVPLRKVGKNFRGLCPFHTEKT
                     PSFYVSPERQTFHCYGCGRGGDIFSFVMEKEGLTFPEALETLAERAGVQLAKRTAGRR
                     SLSDIMELALAFYRENLQGASGEPARRYLESRRLPVAAWSRFELGWAPPAWDGLWAFL
                     RNRGVSRQEALSCGLIVEGQKGDYDRFRGRVIFPIRDMAGKLTAFGGRVLSGEGAKYI
                     NSPEGPLFNKRSLLYLLPAAKGFIRERGEAIVVEGYMDALRLHLCGFQNAVATLGTSL
                     TVEQAALLKRISDRVLICYDSDAAGQEATLRGMSLLQNEGLEVRVVELPRGKDPDELL
                     ASDGGDLLFGQALAKAMPLPLYHVHARRGALAGPGRKKAVEEILNGLAELPMLDLAPH
                     LGSISSALGIFSHQLYELLSQRQGQKKNSPFPEKRDSALSSVLIGERKSNRAEDLPDP
                     LESALLYLLWNCPEKRAGSAPEAILPLFGDERIQSAAAALLNGEPPESLRRIWYETAD
                     RFLESALAAGGDFCEAFGDREEAWQKIEAGLRKRNAEKEYRSLREKMTRGLATSEEMS
                     RLNWLARMLKGRR"
BASE COUNT         5202 a         7483 c         8742 g         5313 t
ORIGIN      
        1 ttatccgcaa gcccggggag ggagtcgcgt ggacgcagag gacgtgccgc tgataccggt
       61 cagaacggtt ggaatcacct tcaacctgaa gaccggccac ggcgaggaag aggaagaata
      121 cgacccgctg gaaaccattc tggccctcaa gagcgagatc gagcgctacg gttttgatgt
      181 cctccttttc aaccaggacg atcttttcct ggaatccctc tcgaaaaatc gacccgattt
      241 tgttttgaat attgccgagg gaaggggcaa cacccgctcc cgggaagccc aggtcccctg
      301 tatcctggaa agcctgggca tcccgtactc cggttcggat gcccttgccc tcgggatgac
      361 tctggataag tacacgaccg gtcaattcct tcgggcccgt ggcgtggcgg ttcccggcca
      421 gttcgttgtg aatcggcttg accggctgga agaccccatg ctggaggcgc ttttcctgga
      481 aaacaggcgc tatatcgtta aaccgcgctg ggaaggctcc tcgaaaggag tttttgccga
      541 ctccgttgtt gccaacctga gccagctgaa ggaaagggcc cgctacatcc tgaaacggta
      601 cgatgagcct gccctcgtgg aggaattcct cccaaacgac gaagtcaccg ttgccgttgc
      661 cggaaaccaa aggccgagaa ccctgggaat catgaggatc cttcccaggg gggaagtcgg
      721 aggggtgttt ctttactcga ttgaacgaaa gaggcaatgg cgtgaatgca tgcgctacga
      781 gaacggcgat gcgctgcccc tcgaggtccg tcggacgatc gaggccgagg ctctgaaagc
      841 ctacgaagcc ctggaactca gggacctagc ccggatcgat ctccgcctgg actgggacgg
      901 catgccccga gtgatcgacg tcaatcccct tcccgggctg aaccccgagt ttggcgacct
      961 gcccatcctg ttcgagctga acggcgggga ttacccatcc ctcatccgca tcctgctgga
     1021 ggaagccttc agccgatgcg ggctgctctg ggccgaaagc tgccgccatg cctgatcccg
     1081 ggcgtgttct cattgccgtc ggactggaac gcaccgaccg gcccgatgtg ctggatacac
     1141 tacggtgcgt ggagtcggtt aaagcggtgt ttgccggcag gggactgccg gttgagccgc
     1201 tctacgtccg atcggaagat ttcccgggcc tggaccgcgt cagggaaacg atcctctcgg
     1261 gctctcctct caagatattc aacctcttcg aaggattcag cggggattcc ggcaaggaag
     1321 tgacgtttgc ttccatgctg gaaaaactgc gggttccctt tacggggaac ccctccgtcg
     1381 cgctggagat gtgtcttgac aaggcaaagg caaaacagcg cctggcaaga cggggaatct
     1441 tggtcccccg gggcgttttt cttcaaaggc tggaaggggc cccctggaag ggcctgaatt
     1501 tccccctctt cgtaaaaccc tgttttgagg atgggagtgt cggcgttgaa aaggacagcc
     1561 tcatccagga cgcggagggg atcgaagccc ttctccggat gaaactggca ggattcccca
     1621 ggggaatcct gctcgaagaa ttcattcctg gaaaagagtt ttccgtcggt tttctcgggg
     1681 ctcctcctta cgaggtgctg ggagtttcga tgatcgatta ctccctctat tcgggaagcc
     1741 tgccgtttct tcactatggc tcgaaatggg atccgcaatg caccgagttt cacgaaatcc
     1801 tgccggtcct ggaggagggg attgccccct ccctgaggga aaggctggtc gacacggcgc
     1861 ggaaggcagg cgaagcgctc gggtgccggg ggtatttccg ggtggaccta cgggaacggg
     1921 aggggcggct atacgtgctg gatgtcaatc caaaccccga tctcaacgaa gacagcggat
     1981 ttgcacggca ggggagaaaa agaggctatt cgtaccctga ggtcgtggaa aggattcttt
     2041 tctatgccaa ttgaccggaa cgattccctg cccggaggtt ccctggagga atacctggcc
     2101 gcggctgagg ggacgggcgc tttccggccc agcgagcttg acgtcctcca ggaagtgctc
     2161 accgatgcct ttgccgaccc ttccaggggc taccttttcc tcgaagagcg gctaccggga
     2221 gggcaactgg cggggtttgc ggttttcggg cgtgctccca tgacggattg ggcatgggat
     2281 ctttattgga tcgtcgtcga acggtccctc caggggaggg ggatcggacg gcgaatgctt
     2341 gagaagctgg aggcaatcgc cctggcggtc acggggaagg tgatcctgag agtcgaaacc
     2401 tccggaagaa aggaatacga aaaacagagg cagttctacc tggcggccgg tttccgggaa
     2461 acgggccgga tccgtgattt ctacgaggaa ggcgacgacc ttgtaaccta ctgcaagttc
     2521 ctccctggtg aaaagagcct ccggtaactc tccagcgcat cggcgaaccg tgccgctcct
     2581 tcccttgact gttccggggc cgctttggca aaggcaaacc ggatcgaatc cgctccttcc
     2641 ccctccaccg ggaagaaaaa ccgtcccggg atgaacccca cctttttctc aagggctgcg
     2701 taccgggcaa attcgagcat gtccgctacc ccttcgcagt tcccccagag gaaaaaaccg
     2761 cctttcggga cgctcagctt gaggcccagt tgggaagctt cgcggtttag cgcctcgacg
     2821 agggaatccc ggcgcttttt gtattcccgg gccagctttt caaggtgacc cccgagatcg
     2881 cacacccgaa ggaactcaaa aacggccgcc tgtgtgaggg ctggggggct cagctcgagg
     2941 ctcagtcgga tctccagcag cttctccttc aggctcaagg ggaaagcgca ccagccgcac
     3001 cgcactcccg gggcgatgat tttcgagaaa cttccaaggt agacgacacg ccggtcttcc
     3061 cctgccatct ctaggtagct tttcgtcggc ttcccttcaa aacggaggta ccggtacggg
     3121 tcgtcctcga gaataaaaaa gcccctcctg cgggccagtt ccaaaatggc ggccttcctc
     3181 gcgtcggagg tcgtgcatcc ggaagggttc tggaaattga cgaccgtgta gaaaaatttc
     3241 gctttcacac ctgccattcg ctcttcaagc gcctctggga ttgggccgtc ctcgtccacc
     3301 ggaaccggaa taatcctggc tccctctctc tggaaggcca gcaatgcctc cgggtatgtg
     3361 gggctttcgg tcaaaatcaa gtcccccggg tcgatgaacg tctcggccaa aaggttcagg
     3421 gctccctgcg acccgttcgt caggatcacg caagaggggt caagccaggc aggtgccagg
     3481 gcttcccttg acatccattc ggcgatccat tccctcaggg gaagatagcc ggcggtgttc
     3541 tgataggaaa atagagaagc ctgtcccgcc gccctgctga aagcctcgct gaggcccgag
     3601 agggggatga ggtcggcgga gggttccccg ctggtgaacg aaatgccgtc ggggcccagc
     3661 attgttgcca tctgggcgat gatggaaggg ccgatgcgcc tcgccttcat gttgaaaaaa
     3721 tcctgcaaat ccataacaaa tccccccgaa tgggcgttgg cctcgtttct cgatttgccg
     3781 ttgtttcgtg cccgctcgat tgaccccatc catactacaa tggaaaaagg atcggggcaa
     3841 aaacagttca acattcgagg agccgaaaga tgatcctgaa agggaagcgc gcctgcggaa
     3901 tcctgcatca tctgacatcg ttgccttctg cggccgggat cggcgatctc ggcgcaggtg
     3961 cgagagcttt tctcgacttt ctcgcggaag ccgggcaaag cctgtggcaa atgctgccca
     4021 ttggccccgt cgaccggggg ctcggcgggt ccccctattg cggggtatcc gcttttgcag
     4081 ggaatgaact cctggtcgac ctgaacgaac tggaagagat cggcctgctt cctccgggag
     4141 aaggaactcc gctctggacc cggatcgacg ccccgatcga ttgggccctc gtcttccaga
     4201 ggaaaggcag cgcgctggac agggcctggg aacgctttcg ggtggggcgg gccgatctta
     4261 gggaggaatt tcacgcgttt tgcagaaaac aggaaaattg gctcaaggac tactcgctgt
     4321 tctgcgcgat caaggcttcc cagggaggga aaccctggaa tgagtggccc cctgatctgc
     4381 gtttgcgatc ccccaaggcc ttgaaagagg cagccgggac ccttgagcag gagagggaga
     4441 gagtcgagtt cgggcagtgg cttttcttcc gccagtggga aaaattcagg tgcgaggcac
     4501 atcggcgcgg gatcgtcctg ttcggtgatc tgcccatcta tgtcggcctg gacagcgcgg
     4561 atgtctggtg ctggcaagag ggattcgacc tgggggaaga cggacagccc cggacggtcg
     4621 ccggcgtgcc gccggattac ttcagtccca cgggacagag gtgggggaat ccgacctatc
     4681 gctgggaggt tcaccgaaag gacgggtttg cctggtggag gtcgagggtg acacatgccc
     4741 tttctctttt cgactgcatc cggatcgatc atttcagggg cctgtctgca tactgggcca
     4801 tcccggcggg agaggagacg gcgcttcgag gaaaatggat cgaagccccg ggagaggaac
     4861 tgcttgaggc cctgctctcg gatcacccga acctgccgat cgtggcggaa gacctgggaa
     4921 tcatcacccc ggatgtcgag aggctcaaga accggttcgg cctgccgggc atgagagtgc
     4981 tccagttcgg ttttttgggc gatacgggga cgaatcccca cgctccgcac aatttcccgg
     5041 aaaatgcggt cgcttacacc ggaacgcatg acaacaacac ggcccgtggg tggttcgagg
     5101 aggaactgga ggaagagggc aaaacggttc tttcccggta cctgggccat ccggtcaccg
     5161 cggagagggt ggcggacgac ctggtccgcc tggcctattc ctcgccggcc gcctgggcga
     5221 tctgtcccct gcaggatgga ctggggctcg atggccgcgc gcggatgaac cagccgggcc
     5281 ggaaaacggg gaactggacc tggaggtgca ggattcccga accggatgtt gctcggaaac
     5341 tggcggaaac gggagtgctt ttcggtcggg cgaggtcaag ccggagtaac gtgctaaacg
     5401 aaccttgatt tagcggcgag aaagcgatac aattaaccaa ccctgaaccc gcggctttat
     5461 tcgcacgttt gatcgacatc acaacagctc aagggggtcg gagagtatgg aggtcctgaa
     5521 agtctcggcc acgtcacaac cgaagtccgt tgcaggagcc atggcagctg tgcttcgcga
     5581 gaagggagtc gtggaagtgc aggcagtcgg cgcgggtgca gtgaaccagg cggtgaagtc
     5641 catcgccatc gcccggggct acgtagcccc gaacggcatc gacatggtct gcatccccgc
     5701 gtttgcgaag atcgatatcg acggggagga gcggacggcg atcaagttcc agctggaagc
     5761 gcgttagggg ttcctgcgga cctaaaaaaa gacagcgggg cgggccccct tcctcgggaa
     5821 gtggggccgc ctttttttgc gaatcgaatg aaacgggcct tctttcgggt aaaatacacg
     5881 tcacgaactc aaaaatgcag ggggtctgga cagatggaaa gcatcaaggc acctcgaggc
     5941 gtgagagaca tcctccccga cgaatcatgg aaatgggcgt tcatctttgg ggtgactcgg
     6001 aagattgcgg aggatttcgg ctaccgggag gttcatcttc cgattttcga gcagacggag
     6061 ctcttttcga gaggaatcgg cgaaacgact gacgtggtcg aaaaagagat gtacacgttt
     6121 tcggaccggt cggggcgaag cctgacgctg aggcctgaag caacggcatc catggtgcgg
     6181 gcctacctgg agcatgggat ggcgagcggc ccgcagccgt cgaaactgtg gtgcgccgga
     6241 ccgatgatgc gctacgagcg tccccagaag gggcgctacc ggcaattctg gcagctggac
     6301 ttcgaggcga tcggttcggc gagtccgctg gtcgacgtgg aagtgatcgc actttccctt
     6361 gagattttcc gccggcttgg cctttcgaac ctggaggtcc tggtcaattc cgtgggatgc
     6421 ccttcctgcc gccccgttta ccggtcagcc ctccaggggt acctgcgcga acggttcgat
     6481 gaactgtgtc cgacctgcca ggggcgcttt gaccggaatc cgttgaggat tctggactgc
     6541 aaggtggaaa cgtgccgcgc catcaccgat ggggcgccgg atgtccggga ccacctgtgc
     6601 ggggaatgcc ggtcgcattt cgacgaggtt ctgcagggac tggatctcat cggggcgaag
     6661 ttcaggatcg acaagcgcct tgtccggggc ctggactact atacgaaaac ggcctacgaa
     6721 gtcctttcgg gagacctcgg tgcccagaac gccgtttgcg gcggcggacg ctacgacaac
     6781 ctggccgaag cgatcggcgg gccgcacacg ccgggcgttg gttttgcggc cggccttgac
     6841 cggatcgtcc tgaccatgga acagcagggg ctcagtttcg gcagggaacc ctccatttcc
     6901 gtcatcgtgg tggttgcgga cgaggccgcc cgcgacgagg ccgtgcggct gctctacgat
     6961 ctgaggcggg caggcgtccc gtcggacatg gatacctccg gcaagggcgt aaaggcacag
     7021 atgaaggccg ctggagccgc gaaggcctcc tgggtgtgca tcctgggctc cagcgaagtc
     7081 gccaggggag ttgtgtcgtt gaaggagatg gcaacgggct cgcaggagga aatcgagcgg
     7141 gagaaggttg tcgaacagct tgccgccaaa acgaaggcgg cctggtgttg aacggtgtgt
     7201 gggaagggag tcctatggcc ttgagtgatc gatatttcga cgattcgtgg aaaaaaacgg
     7261 tctactgcgg gcgggtaggt ccgcaggatg aagggcgtca aaccgtcctg aacggctgga
     7321 tccggaagag acgggacctg ggagggatca tcttccttga gctttgggac tacaccggga
     7381 cggtccaggt ggtcttcaac ccggaggata ccccggaggc ccacgagcta gcgaaggacc
     7441 tgcgaagtga atatgtcgtg gcggtcaagg ggaaggtccg caggcgccca gcggggacgg
     7501 aaaaccccaa cctgccgacg ggagaatggg aactgttagc ggaggatctc ctggtgcttt
     7561 ccccggcctc tccgcttcct ttcgaaatga gcgaagtgga ccgggtcgac gaaaacctgc
     7621 ggctgaagca ccgctacctg gacctgcgaa ggccgaagat gcaggacaac ctccggattc
     7681 gtgcacgggc cgcaaagttc acgagggact acctggtggg acacggtttc ctggaagtgg
     7741 aaaccccgat cctgacgaaa tcgacgccgg aaggagcgcg agattacctc gtcccgagcc
     7801 gcgtaaacca gggggaattc tacgccttgc cccaatcccc gcaacttttc aagcagatcc
     7861 tgatggtgag cggctgcgac cggtatttcc agattgcgaa atgtttccgg gacgaagacc
     7921 tgagagccga tcgccagccc gagttcaccc aggtcgacct cgagatgagc ttcatcaccg
     7981 aaacggatat catcgggctc ctcgaagggt acgtgaaggg cctcttttcg gagatcctgt
     8041 cggaagagat caagacgccc ttcctccagc tcacctggcg agaggccatg gaagcctacg
     8101 gcagcgataa gcccgacctg aggaatcccc tgaagatttg cgacctcgat cccgttttct
     8161 cgggagggac gttcgagccg ttccgaaaga tcctgtcgga aggcggagcg gtacgggggc
     8221 tctgggttcc cggcggagcc tcgctttcgc gaaaggagat ggccgatctc gaggagcggg
     8281 cgaagggcct gggggcccag ggattggcgg ccttccagat caagtcgggg gaactaaagg
     8341 gccccctggt gaagtccctg gacgaagcgg gccgtcaaaa gcttctggaa cggtcgggtc
     8401 tatccgatgg agatgccctt ttcgttgcgg ccggggaatg ggcttcgacc tgctcgatgc
     8461 tggggaccct gaggcttgaa atcgcttcaa aactggacct tgtggaccgg tcctcctggg
     8521 ccttcctgtg ggtgagagat tttccgcttc tggaatggga tgacgaggag cagcgatggt
     8581 gcgccatgca tcacccgttc accgctcccc atccggaaga tgtcgccctt cttgagaagg
     8641 acccaggcag ggttcgttcg agggcctacg acctggtgct gaacgggagc gagatcggtg
     8701 gaggctccat ccggattcat gacccggcca tgcaggagcg ggtcttccag gtgctgtcct
     8761 ttacgcagga gcaggcaagg gaacggtttg ggttcctgct ggaagccctg agctatggga
     8821 ctccgcccca cggaggactc gccatcggct ttgaccgcct ggtagcgctc ctgtgcggct
     8881 gccgctcaat ccgcgaggtc atggccttcc ccaagacggc aaaggcccaa tgcctcatgt
     8941 caggcgcccc atccgaggtg gagagcaagc agctcaggga gcttgggatc tgcatcagcc
     9001 ggggcgaagg acaggcatga aggtctggtc aggcaggggg attcgcgaag gttccaacat
     9061 ttggccatgg cgataccggc ttgagcacga tgactccacg ggcatcgtga ggggggctct
     9121 tgccgtcgag gggtgggagg aatcaaggtc catggcggac tgggctgagg cccgacgcgg
     9181 gggaccgatt cccattacgt tgctttccga cggcggggcg gttgatcttg aaatcgggat
     9241 tcagggcgta cgttcccatg aatccgggca ttacagcgaa acggatctcc aggtagaagg
     9301 gaggattgca ggatgtccat gatccgcttt ttgggacact cagctttcca cgtgagcgcc
     9361 cctggactgg aagggctgat tgacccgttc ctgagggaga acgaccaggc cgtcgtccag
     9421 cccggagatt tcaacagcct gactcacctc ttcgtgaccc acgggcattg ggatcacatc
     9481 ggcgatacga tcgagctggg gctcaagact ggggcgaccg tggtggcgaa cgcggagctg
     9541 tgccgctatt tttccggaaa aggcctgaag acccggccga tgtatatcgg gggctgggtt
     9601 cccttcccct ttggacgggt caagttcgtc cccgcgctgc acggctcagg catcatggaa
     9661 ggggatcaga ccctctatgg tggcgtcgcg gtcgggatcg tcctcgaagt gggcaagttc
     9721 aggatctacc atgccggaga cacgggactg acggcggaga tgggccttct ggaagcgctg
     9781 aaaatcgacc ttgccctcct gccgatcgga gggtgctacg tcatggacat cccggacgcg
     9841 ctgagggctg tcgagatgat ccggccccga agggtcgttc ccatgcactt caatacgttc
     9901 ccgggaattg aggcgaaccc ggatggattc ctggaagccc tcccggaatg gaccgtggga
     9961 atccggcttg atccaggaca aagtctgcag ttttaacggg gcgccaaccg cttgaggggg
    10021 agttcgatgc gaggtcgtct gaaggggaac ccgttttttc tcgcctttct gttcggggtt
    10081 gcattcctga acgccttttc cggaacggca tcggccggag aaattaagct gacttccgat
    10141 acgttgatct acgatccggc aagccggctc gtccgcgccg aagggaacgt ccggctggag
    10201 aggcaagggc tggacctgaa ggcgaacttc gcggaagggg atcccgcggg agagcgtttc
    10261 agggcgtggg gagcagtcag ggcgcactgg gaggagcaga agatggatct ttccgccgag
    10321 gaggtctctc tttttgaccg gccccccagg cgcatccttg cgtcggggca ggtcctgctg
    10381 gagcggcccc gggaacgact ggccgcttct tccgttgaat gggttttcgg gccctccccg
    10441 gaatatgtgg caaaaggcgg agtaagggtg gaagtcccgg gcagggttct cgaggcggcg
    10501 gaggcggggc gaaagaacga tcgtttctgg gcccggcagg tcacgcggtt cgaggaccag
    10561 ggggaaaaga tttccctgaa agccgggcag atcgaagccg ccaccgaggg ggacagcttg
    10621 aaggatctga cggcgaccgg acaggtgtcc gccgtcgttg taggttcgga tggggttccc
    10681 gtcaagctga agggggatcg actcctgtat tcgaaaggga aaaatacggt tgctctggtg
    10741 gggaatgccg aggcggtcca gtccggacgt agtgtccgct cggaaagcat cctttacaac
    10801 ctggccaccc gaaggatcga ggcgcagggg cgtccccagc tgattttcac accgggcaag
    10861 gagacggcgt caccgtgacg tcttcgcgcg tgcttcgtgc ggtggagctc cagaaatcgt
    10921 acaagggcag gcaggtggtc ggttccgttt ccatcgaggt ccggcagggc gagatcgtcg
    10981 gtctcctggg accgaacggg gcgggaaaga cgacgacttt ttacatgatc gtggggctcg
    11041 tgcgtcccga cgctggggag atcctgatcg acgaccgcga gatcacaacc cttccgatgt
    11101 acaaaagggc ccgcatgggg gtgggctacc ttcagcagga agcctcggtc ttcagaaacc
    11161 tgaccgttcg ggagaacctg gaactggtcc ttcaggagct ggaaaaaaca ccggcggagc
    11221 gagccaggat gatcgaccac cttgtcgccg agttcgggat cgaccgcatt ctggacactc
    11281 ccggatatgc cctcagcgga ggggaaagga gaagggtgga gctggctcgc tgccttgcgg
    11341 cgatgcccga attcctcctg ctggacgagc ctttcagcgg aatcgacccg atcgcggttt
    11401 tcgatatcca gcagatgatc ctgggactgc gcgggaaggg gtacgggatc ctgctgacgg
    11461 accacaacgt gagagacacg cttgcaatca cggaccgggc tgcgattctc caccagggcc
    11521 ggatcatggt tgaaggaaca ccccgggaag tctcccagag cgaggtcgcg aggaaatact
    11581 acctcgggga tcgttttacg ctatgaaggg tttggcgtga agcgcctcca cgatccttcc
    11641 cggatgcatc gaaccacctc acgggcggcg gcctcgacct cggggctgag gtcaagagaa
    11701 aaaccggttt ctttcggctc gatgccgata acggcaattc tttcccgggg aagggcttcc
    11761 ctgaggattt gccccagcgg tagtccgtgc gtggtttccg taagaccgtc gaggcaatcg
    11821 ggatcgagga ggcgaatcgt tcctggggcc gtccccatgg cgcaggcatc gatgatcacg
    11881 aggaagtccg ggggattcct gcgaaggggc gcggaaaagt tttccgggac cgtttcgcaa
    11941 aggacgatct cgagatgagg acaaggcgtt tcaagcagca gttccccgca ccggacgccg
    12001 accccgtcat cgccccggag cggatttccc aggcaccaaa ccgttgtccg ggaatttccc
    12061 acggcgtttt cccctttcat tctggttttc tggagggtcc tgaatgtctt ccccgttgag
    12121 caaaatggtt cgacatgcca tgagaatgat ggtgggcacg ttcgccagcc gcatcctggg
    12181 actgctccgg gaaatcatca ccgcagccct cttcggggcc acccggcagc ttgacgcctt
    12241 tttcgtggct tacacccttt ccaatctttc ccgccagctt ctcgccgaag gagccctttc
    12301 ggccgctttt gtcccggtgt tttccagggt tgtcgtggaa gaagggcggg aaaaggccgc
    12361 caagctagcc cggcaggtga cgaccgtcct cctcctggcc ggcagtaccg ccgtaacggt
    12421 ggggatcttc gccgctccga tactcgtccg ggtgatggca ccggggtttt caggagcaga
    12481 cacacagctt gccgtgcttc tgacccggct gatgttcccg tttctgcttc ttgtttcggt
    12541 ggcggcactg gcaatggggg ttctgaacag ccttgacagt ttcttcatcc ccgcggtggc
    12601 tccggccctc agcaacctcc tctacatcgt tcttttggcc attctagcac cgaggatggg
    12661 gatctgggcg ctgccaacgg ccgtactgtg cggaggagtg gcccagatgc tcctgcaatg
    12721 gtggcgttcc ggggcgctgg gggttccgct ccttcccgca aggcccgatt ggaacaatcc
    12781 ggaactgaaa agggtgatcc gcctcttttt cccgtacatg gcgggtcttt cgctgaacca
    12841 ggtgaacccg gtcatcagcc gcatggtcgg atcgtttctc gagggaggca cgatctccgt
    12901 cctgagttac gcggaccgaa tcatccagtt gccccttggc ctgtttgtca tcgccatctc
    12961 gcaggccgtc ctgccgatgc tctcgaggat tggggccgag gacaccgctg gttttgccca
    13021 aatgacgcgg gatgccctcc ggttcaccct gttcgtcatc ctgccggtgg tcgtcgggat
    13081 gagccttctg gcccgcccca tggtccacct gatttttgtc cggggagctt tcaacgaatg
    13141 ggcctgggag gcaacaagcc gggctttggc tctctatgcc ctcggcctgc ccgggatggc
    13201 ctgctccacc gtggtaatga gggggcttta tgccaggagg ctgccgaggg aggctttgaa
    13261 ggtcaccggc tttacggtgt tcctgaacct cgttttctgt gtcaccctgg tccgtttttt
    13321 tgcctacgcg gggctagccc tggcaacgtc ccttgctttc agcggaggag cgctcatggg
    13381 ggcatggacg ctccgccgcc acctggaaac cccgatcggg gtcctgaccg gggaatggct
    13441 ccttcgccac ggggttcccc tggccttgat ggctgcggga atccttgttg gggcccgtct
    13501 tttcccctac gatccggctt tcaggctgtg gggaaagctg ttctgggtgc tcggtgccgt
    13561 ttcggcgggt gccgtcctgt acgcggttgc ttcctggatg agcggttgcg ccgagtggaa
    13621 atgggtttcc ggagccttcg gaaaggagga acgagtccat tgagggaaga agggagaaac
    13681 gcaggtggcc ttcccgttgc cgtcgtgtca aggggattgc tggccggagg tcgtttcagg
    13741 atttttgccg tcaaggccga ccggctggtg gatgagacga tgaggcggca gaacctcagt
    13801 cctcttgcag ccgcagccct tggacggctg atgatggccg ggatgatgat ggcgctcgac
    13861 atcaagctca gcaaagcgag ggtgaccctc aagctcgacg ggggaggccc tctcgggctc
    13921 gttgtcgcgg acgcggaacc gcaaggacgg gtacggggct acgtggaaca tccggaagtg
    13981 gatctgcccc cctcgtcctc cggtaagctg gacgtggctt caggagtagg caaaaacggg
    14041 acgcttgtcg tgttcaggga cgtccggatg aaagaaccgg tttcgagcca ggtccccctc
    14101 gtttcgggag agatcgccga agatatcgcg gcgtactacc gcttttccga gcagaccggc
    14161 acggccgtcg gccttggagt gctcgccagg ccggggcagg tgatttcggc cggagggttc
    14221 atggtgcagg ttttgcccgg ctcggaggac tcgcaggtca gcatcgtgga agaaagggtc
    14281 cggaacactc cttcggtgag ccgtttgctg gaggagcatc ccgatgcaag aagcgccatg
    14341 gaggccctgt tccgggagtt ggacatcgaa tggttcgagg acgtggaatt ttctctctct
    14401 tgccggtgca gccgggatct tgcgaaagcc ctcctgctcg aatccctcgg gaaggaaatt
    14461 ccaggggaga ggcaggaagt ccgttgccat ttttgcggga cggtctacac tttctcgaaa
    14521 gaggaactgg aaagggactg gcccccggag gcgggaggag caaatgaaga atgaaggagg
    14581 aggaccagca gaatgaaaaa agcactctgg atgtgcgttt tcctgttcat cgccgccgtt
    14641 ggaggggttg cctggggagg acaggcgaag agccttgatg acctcctggc tgagcggtct
    14701 gccaacgtct gggtggacgg gcaggttctg ggagacctcg ttctgggagc gaaagcgcgt
    14761 ctgaccttca tttacgttga tcccctcctt gcctcggcag cgcgcaatga tccaggggcc
    14821 ccggaatggc tgacctggca ctcgaggcac ctcggggaga agaacctcaa gggaagggcc
    14881 ctttttttac ttcactttga aacctttacc ccctgggagc tggatcccgg aagaatccat
    14941 gtcggaaatt acaccgtttc ttcggaggat gttctttcca tgagggaata ttccctcacc
    15001 ggggagctcc cgagcggttt tacgggaacc ctgccgttct ccgtcccgaa atctttcctt
    15061 aagggcggga cacagatcgt tctggcttat ggagaggagg attcggccca atggaaagtg
    15121 cccggcaaat gagcgggatc catgtctatc gatgtgcctc ctgtgaccac cgtttcgagt
    15181 cggaggattt cgccagccgg tgcccgaaat gccggagcaa ggtgcttttg catgaaagcg
    15241 gcccggcaag gactgcgaaa aagggttgcc ggggtcgttc ctgcggaggc tgtggggggt
    15301 gcggggggca ttgacctcca ggcggttcct gactctcggg atcgaaagca gctgtgacga
    15361 cacggcggtt gcggttctgg aaggcccgac aacggtccgg tcgtccagaa tttcgagcca
    15421 actggaactt catgccccct atggcggagt tgtcccggaa ctggcttccc gtgatcacca
    15481 ggagcggatc ctccctcttg ttttttcggc cctttcggaa gccggcgtga cggaccccgg
    15541 cacagagctg gccctgatcg gcgttacgat gggaccgggg ctgatgggtt cgctgatggt
    15601 cggcgtgatg accgccaagg cgcttgccca ggcctggaaa gttcctttgc tgggggtcaa
    15661 tcacctggag gggcatctgt tcgccaacgt gatccagaat ccgggcctga agcctccatt
    15721 tttgaccctc cttgtttcgg gggggcacac tgaagtgatc ctggcgaggg ccttcgggga
    15781 ttacgcggtc ctgggctgca cacgggacga tgccgcggga gaagcctacg acaagctggc
    15841 caaggtcctg gggctgggat accccggcgg gcccgccgtc gaccgtctag cggcaaaggg
    15901 aaatcccgac gcgttccctc ttcccgttcc gctggcggag acttcccgcg ttgagttcag
    15961 cttcagcggc ctgaagaccg cctccttgaa tctcatccgg cagctggagc gcgagggacc
    16021 ccttccggtt gaagatatct gcgcatcctt ccagaaggct gtcgtcgagg ccctgctgaa
    16081 aaaggtcgag ctggcggttc agctgacagg ggtccggagg attgccgtgt ccggcggcgt
    16141 tgcggcgaac agcggcctga gaagggcctt ttccgggaga aaggactggg aggtgttcat
    16201 ccctccggcg atcctatgca cggacaacgg agtgatggtg gcggccgcag cctatcatgg
    16261 ctatcggcgt gggaagagaa ccgggctcga tttttctccc gatcctgccc tggaactttt
    16321 atcggattga aagtaagaga aaaaacgaga aaaacggacc taggtgagca aaaaaagcct
    16381 aggttcgtct tttttagctt agagtccgtt tgagagaaca ccgggtcccg cctacactct
    16441 tctccgggaa attagcactc gaccctagtg agtgctaata cgataaaaca attcgtagga
    16501 gggatgttgg atgcacctga agccgttggc ggatcgagtc gtcgtcaagg tcctgaccct
    16561 ggaggagaag acgcgtggcg ggatcgtgct cccggacacg gccaaagaga agccccagga
    16621 agccaaggtc atggcggtgg gttcgggaaa ggtcctggag aacgggcaga agctggctcc
    16681 cgaagtcaag gtcggtgacc gggtcctttt cggaaaatat tccgggactg aagtcaaggt
    16741 tgacggcgaa gagtacctga tcctgagcga gcgcgatatc ctcgcgatcg tcgagtagac
    16801 cccgatatct tcgagaacag gagggacatt gacatggcca agatcctgaa ttttggtgag
    16861 gatgcccgcc gctcccttga aagaggcatc aacaaggttg cagacaccgt tggcgtgact
    16921 ctcggcccca agggaaggaa cgtcgtcctt gaaaagaaat tcggttcccc caccatcacc
    16981 aatgatggtg tgacgatcgc caaggagatc gagctggaag atcccttcga gaacatgggc
    17041 gcccagctcc tgaaggaagt cgcgtcgaaa accaacgacg tggccggtga cgggacgacc
    17101 acggcgaccg ttctggcccg cgcgatgatc cgcgagggca tgaagaacgt tgcggcaggc
    17161 gcgaacggca tcctgatgcg ccgcgggatc gagggcgcgg tcgacgtcgt cgtcgatgag
    17221 ctaaaggctc tctcgatcgc cgtcaaggag cgtagcaaga tcgcccaggt tgccgcgatt
    17281 tccgccaacg acaaggtgat cggcaacctg atcgccgaag ccatggaaaa ggtcggcgag
    17341 gacggcgtca tcaccgtcga ggatggacag accgtgggca cgaccctcga gacggtcgag
    17401 ggactccagt ttgacaaggg ctacatcagc ccctacatgg tcaccaacgc cgagaggatg
    17461 gaagccgttc tcgaggatgc ctacatcctg atcaacgacg gcaagatcaa caacgtgaaa
    17521 gatctcctgc cggtgctcga gaaggtggtt cagaccggca agcctctcat gatcatcgcc
    17581 gaggacgttg agggcgaggc ccttgccacg ctggtggtca acaagctgcg cgggatcctg
    17641 caggtcgtcg cggtgaaggc tcctggcttt ggcgaccgcc gcaaggccat gctccaggat
    17701 atcgcgatcg tgaccggagg ccaggtggtc agcgaagaag tcggcctgaa gctcgagaac
    17761 gcggacctgt cgatgatggg ccgggcgaag accgtccggg tcaccaagga agaaaccacg
    17821 atcgtcgagg gcaaggggga tcccgagaag atccgtgccc gcgcagccca gatccggaag
    17881 gaactggaag actccacttc cgagtacgac aaggagaagc tgcaggagcg ccttgcgaaa
    17941 ctggtcggtg gcgtggccgt cattcaggtc ggagctgcga ccgagaccga gcagaaagaa
    18001 ctgaagcacc gcatcgaaga tgccctgaac gcgacccgcg cggctgtgga ggaaggaatc
    18061 gtcgccggtg gcggcgttgc cctggtgaac tcgctgcagg cccttgacaa gcacatcgag
    18121 accctttccg gcgacgagag gacgggtgcc acgatcgtcc gcaaggccct gaccgaaccg
    18181 cttcacctga tcgcctccaa cgcgggctac cagggtgacg ttgtggtcga gaaggtcctc
    18241 gggcttccca aggggcacgg gctcaacgcg gccacgggcg agtacctcga catggtcaag
    18301 gaagggatca tcgatcccct caaggtcacc cggagcgcgg tgcagaatgc ggcatccatc
    18361 gcggccatgg tcctgaccac ggaagctctc gtcggtgaca agccggagaa gaaggaatcc
    18421 atgccctcca tgcccggtgg aatgggagat tacgattaat cgaagggttt tcccaaggcc
    18481 cccgcttcaa gcgggggcct ttttttctcg ggacggttct ccctccgggt ctttgtctgt
    18541 taagattagc aggaccaccg tcaacccgat tccaagccga agggagagaa gccctaaagg
    18601 ggctctttta tggcgccaca ttcaaacatc gtcatcctgg actgcggctc tcagtacacc
    18661 cagctgattg caaggaggat ccgggagctt tccgtccata gcgagattct tccgtgggat
    18721 tctggcgcgg aggagatcct gtcccacaat cctaacggaa ttgtcatttc gggcgggccc
    18781 cggagtgtcc tcgaggaagg ggccccggtg atcccggaag cagttcttca ttctggaatc
    18841 ccgatcctgg gaatctgcta cgggatgcag ctgctggctc acctgctggg ggggaccgtc
    18901 aggcaggccc ccctggcgga gtatgggccg gcggaagtcc ggattctgga ttcccggaca
    18961 cccttgctta aggggctgcc ggaaacgctc tcggtctgga tgagccactg ggacagcgtg
    19021 atctcgcttc cctccggttt ttcggtcatc gcagagagca gcggcggact gatcgccggg
    19081 ttttcggacg aaacgggccg gatctcggga ctgcagtttc atccggaagt ggcgcacacc
    19141 ccgcaaggga tggagatcct gcgaaacttt ctgttttcag tctgcggatg cgtggcggac
    19201 tggaacctca gcgactgggc cgagcaggcg atccgtgcca ttcgggagca ggtggggagc
    19261 gaccgggtca tctgcggcct ttccgggggt gtggactcta ccgtcgcagc cgttctgacg
    19321 gcccgtgcga tcggggatca gctcgagtgc atcttcgtga acaacgggct gctgcgtctg
    19381 aacgaggcgg aggaggtcct cgaggcctat cgcgagatga agctgaaagt tcactatgtt
    19441 gacgcaaccc ggcgattcct ggaagggctc aagggggttt cagagccgga acggaaaagg
    19501 aagatcatcg gggaactgtt tgtccgggtt ttcgaagaga aagcggggga aatcggtgga
    19561 gctagctggc ttctccaggg cacactctat ccggacatca tcgaaagcgg gcacaagggg
    19621 aaaggggctg cggtcattaa aacgcaccac aatgtcggag ggcttcccga gagaatgtcc
    19681 ctcaaagtcc tcgaacccct gaaggacctg ttcaaggacg agggaaggga aattggcagg
    19741 atgatcggcg tgccggaggc catcctgaaa cgtcacccct ttccggggcc tggcctggcc
    19801 gtccgctgcc tgggggaaat caccgcggag cggttggaaa cgctgcgcaa ggcggacgcg
    19861 attttcatcg aggagatccg gaaagccggg ctttatgacc gcctgtggca ggctttttgt
    19921 gtccttcttc cggcacggag cgtcggggtg atgggcgatg tccggaccta cgccgaggcg
    19981 gtcgcccttc gcgcggtcga gtcgcttgac gcgatgaccg cagactgggc gcggctgccg
    20041 gccgacctgc tgaatcgagc ggccaaccgg atttgcaacg aggtcccggg cgtgaaccgc
    20101 gtcgtctacg atgtcacgag caaacccccg gcgacgatcg agtgggagtg aggggaaagc
    20161 gggaaaaagg cggaagaggg catgagtcga acctccaaag acgctttccg cgatttcctg
    20221 gaacaggtcc gctgcggccg tctttccgtt gatgaaggcc tggaagtgct ttcgtcaatg
    20281 gcgacccttc atttggaaga aatccgcctg gacagccacc ggtccttccg gtgcggcatc
    20341 ggggaggtca tctactgccc ggggaagacg cgccttcagc tggagaagat tgcggaggcc
    20401 tgccgtgaaa ggcccgaaac cctgattttc agccggatgg aaagggagca ggccgaattc
    20461 gtgagagagc ggcttcaagg gttttgctac gacgaggtcg cccggactgg ctacaggaaa
    20521 ggggaccccg cggtcgagcg ggggtgcgtc gcggtcatca gcgggggcgc cgccgatcag
    20581 cgggttgcgg ctgaagcggc tttggtggcg gagctttgcg gttgcgaggt cgcccggtat
    20641 ttcgacgtgg gggtcgctgg actccatcgc ctgctgtccg tgttgccgga gctgcgaaag
    20701 gcggatgtcg tcatcgcggt agcgggaatg gaagccgcgt tgccgactgt cgccgcgggc
    20761 ttgctgaata cggtggtcat tgcggttccg acttcggccg gctacggggc gaacctgggg
    20821 ggcgccgccg ccctgctcgg ggttctcaac agctgctgtc cgaacgttct ggccgtaaac
    20881 atcgacaacg gtctgggagc cggaatttct gcttctctga tcgcgggagg gggaagccgc
    20941 cgtggttgaa gggcctagcg cagaaacgtg gaaaaacaag ccaaggggaa cgtttgtccc
    21001 cttcgtcgtc tttttcgccg cactctcgac gatcgcgttc cttttggcct ttcccctttt
    21061 gcgtcccctg gcctggtcga tcatcctttc cttcttcagc tatccgcttt ttcagtttct
    21121 tcatgatcgg ctgttccgcg ggagactggc caacctgtcg gccggattga ctaccgctgt
    21181 catcattgcc atgctcatgc ttccggcgat cctgttcggt ctttatctgg cccatgacgg
    21241 gatggccctt tacgggcgcc tcaccacgat catcgaaaaa ctggaaaagc aggatctttc
    21301 cctcattttg aatctcatcc ccccgagcgt ccgggaattt ctcgcccctt ggctttccca
    21361 ggtcaacctg ttcggcgatc tgttcgggca gctcggaagg tggttttcgg gctggattgc
    21421 cggtgtctcg cgcgggctat tcgaaagcgc cctccgcctg gttttccaga tgatcgtcat
    21481 taccgtttct tccttcttca tgatccggga cggacatgtc atcgtcgagt acatccggga
    21541 catcgttcct ctttctgcgg aggagacgga cgcgatgatt tccaggggcc ggcggatgct
    21601 tcaggccgtg gtctacgggt tcacgttcac agcggccgtg caggggttcc tgggagccct
    21661 cggctggtgg tgggtcggcc ttccaaatgc gttgattttc ggggttctaa tgttcatttt
    21721 tgcgattatc cccttcgtgg gaacagccct ggtctgggtg cccggggccc tgtatctttt
    21781 tgccacggga cagcacgggg ctgccctgac ccttgccttc tggggactgg ccgtcgtcag
    21841 caccgtggac aatttcctgc gcccgatttt catctccgag ggaagcaagg tccacatcct
    21901 gatcgttttc gtgggcgtcg tgggaggtct tgcgacgctg gggctgctgg gaattttcct
    21961 gggtcccctg gtcatgaccc tgttcttctt ctgccttgac gcctaccgca ggatttggat
    22021 gagcctccgc caggccgccc agccggagga gccaaggcga tgatcccggt cctggccgtc
    22081 agcgggttca agaaatcggg gaaaaccacc ctttgcagag agctggctgc ttgctttcag
    22141 gaggttggag tgcgcgtcgg tttcgtcaag aggacgcacg aaccctccgt ccgctgcggg
    22201 ggaacggaca ccggcctcct cctggaagcg ggcgttccga tagtcctctg ggcccccgac
    22261 ggccttcaag ctgaaaacag ggaggagcag cccgagctgg actggatggt cgaacggttt
    22321 atgcccggtg tggatctcgt gcttcttgag ggaggaaaga gcctttccct gccgcgggtc
    22381 tgggtcggaa accccgagga atgcccggca gaagtcaagg ggatcctggc ctggtttggg
    22441 gcttttccgc ctgcgggcag gatcccttca tttcggccag ggcaggagaa ggatcttgcg
    22501 gcatacctcc tcgatcggct tctggggaaa aggcagtctc cgtccgtcag cctgcatgtc
    22561 gacggccgga agatccctct caagccctac ctcgaaaggt tcctgggaca ggccatcctg
    22621 gggatgattc gttcgctcaa ggggactcag gggcgcgata tctcgatcca tatccggaac
    22681 tagggcgccg ttttgaattg aaggtttttt ctgacgcctt gtgcggaatg gacgggatga
    22741 ctataataat ggacgctttt caggccgatg cccgcccgga atgggcgggc aagttgttac
    22801 accgaacgga atctttttca gtctaaagga ggtttcatcc atgggaacac aggtcatact
    22861 gctggtggct ctggccgggg tcctggccct ggtctacgcg gtttgggcta cgggcaggat
    22921 caacgctgtc aaggtcgata acgagcgggt caacgacctt tccggcatca ttcacggcgg
    22981 ggccctggcc ttcctctaca gggaatacaa ggccctcgtc ccgtttgtgg ttatcgtcgg
    23041 cgggctgctt ttctggaaga tcgggaccgg gtctgctctc tgctttgtcc tgggcgcttt
    23101 ctgcagcgca atgaccggtt actttgggat gaacgtcgcc acgaaggcaa acggaaaaac
    23161 ggccttcagc gcaatgaagg ggatgaacca ggcgctcacc gtggccttca ccggcggttc
    23221 cgtcatggga atgaacgtgg tgggaatcgg aatcctcggt atccttctct gcacagtgat
    23281 tttcaaggac ccgaacatca ttgtcggctt cggtttcgga gcaagctcca tcgctctttt
    23341 cgccagggtg ggcggcggga tctacacgaa ggccgccgat gtcggggcgg acctggtcgg
    23401 caaggtcgaa gcgggaatcc ccgaggatga ccccagaaac ccggccgtca tcgccgataa
    23461 cgtgggtgac aacgtcggtg acatcgccgg aatgggagcc gaccttttcg aatcctacgt
    23521 gaacgcgatc gtggcggcca tggcagtggg gatcgtgacc ttcgggaatg ccggcatggt
    23581 ctatccgctt cttctggcgg ctctgggcat cctatccgcc atcctgggga ccttctttgt
    23641 gaaggtcggg gaaaacgggg atccccaggc ggccctcagc cgggggacct acgcgacagg
    23701 ggcgttcctg atcgttggga cctttttcct cacgcgggcg atgttcgggg aattgaccct
    23761 tttctacgcc gtggtcgctg gcacgattgc gggtgttgcg attggtttcg caacggaggt
    23821 ctacacctct tcctcctaca agcacgtcaa ggagatcgct gaggcttccc tcaccgggcc
    23881 ggcgacgaac attctctcag gcctgggaat cggcatgaag tccaccgcag ttccggttct
    23941 tctgatttgc gccgcgaccc tggtcggggt gcgtttcggg ggcctgtacg gaatcgcctg
    24001 ctctgcggtc ggcatgctgg cgatcactgg aatggtcctg agcgtggacg cttacggccc
    24061 catcgcagat aacgccggag gaattgccga gatgagccac ctgcctcacg aggtccggca
    24121 gatcaccgac aagctggatg cggtcgggaa taccaccgcg gcggtcggaa aaggactggc
    24181 gatcggttcg gcggcgttga ccgcgttggc cctctttgtc gcctatgccc aggctgccca
    24241 tatcgacgtc atcgacctgc gcgacccgaa agtgatggtg gggctgttca tcggcgggct
    24301 gcttcccttt attttcgcgg ccctttccat cggagccgtc ggccgcgcag cccagcacat
    24361 gatcgacgag gtacgccggc agttccggga gattccgggc atcatggagg gaaccggccg
    24421 gccggagtat gagcggtgcg tggatatttc cacggcggca gccctgaagg agatggtcgt
    24481 tccgggactc atggcggtca ttgcaccggt gctcgtgggc cttgccctgg gggcgggggc
    24541 tcttggcgga ctgctcgggg gttcgatcgt gacgggggtc atgctggcga tcttcatggc
    24601 gaactccggc ggggcctggg acaacgcaaa gaagtacatc gaagaggggc accacggtgg
    24661 caaacgcact ccggcccatg ctgccgcggt tgtcggggac acggtcggcg accccttcaa
    24721 ggacaccgca gggcccagca tgaatatcct gatcaagctc atgtccgttg tggcggtggt
    24781 cattgccccc ctgttctcct aagaaagaag cagaaatgtc ccctggcggg tgaacgggcc
    24841 ggggatccgt taaaagcaat cgcgatgagg ggggcgaaag agcccccctc atcgcctcca
    24901 gtctggagga ggtgtcggaa tgggccagga tgaagccgtc cgggagatca aggaaaggct
    24961 cgacattgtt gaactggtcg gtgatgccgt ccccctccgg aaggttggaa agaacttccg
    25021 gggcctgtgt ccctttcaca ccgaaaagac cccttctttt tacgtttcac ccgaaaggca
    25081 gacctttcat tgctacggtt gtggccgcgg gggagacatc ttctccttcg tgatggaaaa
    25141 ggaggggctg accttcccgg aggcactgga aacccttgcc gaaagagccg gggtccagct
    25201 tgccaaaaga acggccggca gacgcagcct ttcggacatc atggaactgg ccttggcctt
    25261 ctatcgggaa aacctgcagg gggcatccgg ggagccggcc cggcgatacc tggaaagccg
    25321 aaggctcccc gttgctgcct ggtcgcgttt cgagcttggc tgggctccgc cggcctggga
    25381 cggcctttgg gcttttctaa ggaacagggg ggtttcaagg caggaggccc tttcgtgcgg
    25441 actgatcgtg gaaggacaga aaggcgatta tgaccgtttt cggggccggg tcattttccc
    25501 catccgggac atggccggga aactcacggc ctttggcggg agggtcctgt cgggggaagg
    25561 cgcaaagtac atcaatagcc cagagggccc cttgttcaac aaacgcagcc tgctttacct
    25621 gcttcccgcc gcaaagggct tcattcgcga aaggggagag gcgatcgtcg tcgaggggta
    25681 catggatgcg ctgcggctcc acctctgcgg tttccagaac gccgtggcca ccctcggcac
    25741 ttccttgacg gtcgagcagg ccgccctgct caagcggatc tccgaccggg tcctgatctg
    25801 ttatgacagc gatgccgcgg gccaggaggc gaccctgagg ggaatgtccc tcctgcagaa
    25861 cgaggggctg gaagtgcgcg ttgtcgaact gcccagaggc aaggatcccg acgagcttct
    25921 ggcttccgat ggaggcgatc tgctcttcgg gcaggctttg gcaaaagcga tgcctctgcc
    25981 cctgtaccac gtgcacgcga ggcgaggggc cctggcgggg ccgggccgga aaaaagccgt
    26041 cgaggaaatc ctcaacggcc ttgcggaact tcccatgctg gacctggctc cacacctggg
    26101 atccatctcc agtgctctcg ggattttctc gcaccaattg tacgagctcc taagccagcg
    26161 gcaaggacag aaaaaaaact cccctttccc ggaaaagagg gattccgccc tttccagcgt
    26221 attgatagga gagaggaaaa gcaaccgggc cgaagaccta ccggaccctc ttgagtccgc
    26281 gctgctttac cttttatgga actgccctga aaagcgcgcg ggaagcgcac cggaggcgat
    26341 cctcccgctg ttcggggacg aacggatcca gagcgcggcg gcggctttgc tgaacggaga
    26401 gccgccggaa tccctcagga gaatttggta tgaaacagcg gaccgtttcc ttgagtccgc
    26461 cctcgcggca gggggcgatt tttgcgaggc attcggggat cgagaagagg cctggcaaaa
    26521 aatcgaggcg ggtctcagga agcgaaatgc agaaaaggag taccggagcc tgagggaaaa
    26581 gatgacgcgg gggttggcga cgagcgagga gatgtccagg ttgaactggc tggcccggat
    26641 gctgaagggg aggagatgaa gacgatggcc aaaaaggaaa aaaaagaaaa gaagcatccc
    26701 gatgcggtgc aggaggagaa ccttcgcgaa agcggggaaa
//
LOCUS       DAOOVN010000014        23270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_1495_length_23270_cov_13.756192, whole genome shotgun
            sequence.
ACCESSION   DAOOVN010000014 DAOOVN010000000
VERSION     DAOOVN010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 23270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23270
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1495_length_23270_cov_13.756192"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="PLY83_02765"
     CDS             complement(<1..1007)
                     /locus_tag="PLY83_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HOV28991.1"
                     /translation="MPKPNREGCPAECRGCSHRELDEAESLAQKQAWLADRLAPWASV
                     LRRIVAPAGPERWGYRDKVSLKARWDPQEGWRFGMEVRDRLVGLENCPVQSIRVRSAL
                     AWLSKALPPAGVFPLSIYSQVGAQATLILKTQNDPGDGWLAGKEAELALTGLEGLWVH
                     LFPCAGRHLFGAGPMRLLWGKPLSRNPMGLFYGPQGFQQVILSLYARSLDEAEAFLSP
                     GPGTSVIDLYCGAGATLARWSARGARTLGIELSKAALDCARLNAPEADLLRGRCALRI
                     PQAEAWIQQVPEAFRLVYANPPRTGLEPEITEWLATRCRPARIAYLSCSAGTLARDLE
                     R"
     gene            1222..4374
                     /locus_tag="PLY83_02770"
     CDS             1222..4374
                     /locus_tag="PLY83_02770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOV28992.1"
                     /translation="MSCPPALSPFLVLLPLLLSASPARADSAGLSPRGFSLPVVLGLV
                     LAAALGSFFAGRQTGLRGLPADEGASRGAEASQAAVLEKTDQAEEPPPPSSEKKEVPG
                     ERTELLLEAEELARMGFFRVDLETGSVEASPFLKKLFGFDPDEALTFQDYESRIHPDD
                     RSRVRAAREQAFSAGEDLYDLRYRVSPGSRVIHQVSARVHVIRNPNGRPSGLFGVIQD
                     LSEREEMENRLRVSQELFRQYMENAPSALIMASPQGRIQDVSPSAERLFRLPREQLLE
                     MTLLDLFPQNYVDEGRKRLRELAARGHLSVEVPFRTSTGKAGWGRWDAVSLSKGAYLV
                     FLTDLTQEHEAQEALLHSEAAFKRLAENLPTLIFRVDEDFRFVYLNPSTERFFGKTSE
                     ELQCKTLADSGLPSAFLRQWTERLKDVFRTGQGMRFEVNLDTGATVRYLQTVLVPEKA
                     DLGIVRSVLAVSTDITEIKRQEARTQQQRDFLDALIRNAGEGIAVFDSTGRFLVFNPA
                     MEAMTGWKLEELQPPAISYSFSRGEGAAEAASGILKALEGVASEEERLLPCRGDVLKT
                     VMVSSSPLSGNPEALTLVLVRDITEQKRATEALARERAYLDMLFENAPDGMVVTDRFG
                     QILRLNRRFSEMFGYERQEALGRQIDTLVVQGASLQEGKTLTRQVASGRTVSIETERI
                     RKDGTTFPCRAIGIPIRLEGGDVHLYILYHDLTEIRQREAELRLANEVVKSSPVILFR
                     WGNGTGSPIEYVSENVRLWGYEPEELVSGRKLYSELIHPEDREKTLQEVRDFVSQGRD
                     EFQQEYRVVKKNGETIWVNDQTRVVKTAEGSIAFYQGVVLDITEKKRFELLLEENYQT
                     MRRAWDETIGVLSTTVEMRDPYTAGHQRRVARIAGAIARKMGVPEQILFAVERASLLH
                     DIGKIEVPAEILSKPGSLSAIEMGIVKQHVEAGWKILKDIDLPWPLAEIVYQHHEFLD
                     GSGYPRGLKGEEICLEARIITVADIVEAMASHRPFRPSRGLEAALSEIEHQRGITLDE
                     RAVDACLELFRQEGFTLGGN"
     gene            4405..5670
                     /locus_tag="PLY83_02775"
     CDS             4405..5670
                     /locus_tag="PLY83_02775"
                     /inference="COORDINATES: protein motif:HMM:NF019244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HOV28993.1"
                     /translation="MEDSGTTPAGNRLHYGILVSTFLSLVLAGFLFDTPSGIVKGLLK
                     IVTDPDYLITDYMGIGGVGAAFVNSGLVSLLCVALLGFLKVQVTGITFAAIWTVAGFS
                     FFGKNLLNVWFIIAGVRVYALYRKEPFSRFIYSALFGTALAPLVTQILFGFNVSPALR
                     IPLAALAGISVGFILPPLGVAMMRFHQGFNLYNIGFVSGLVGSIVLSIFRSYGFIPQS
                     RLVWTKGHDLQLAPLFFAFFLAHFALGYFLGGRKVSGLAAVMRYPGRVVTDFILLEGL
                     GPTLMNMGLGGMASILYLLAVRGDINGPTLAGIFTVVGFSAFGKHLRNMAPIVAGVVL
                     GGATKIWGMNDPPIQLAALFGTTLAPVAGEFGIFWGIVAGFLHSSLVLNVGALHGGFN
                     LYNNGFSGGIVAAVLVPVIEAIRTGREKS"
     gene            5667..6014
                     /locus_tag="PLY83_02780"
     CDS             5667..6014
                     /locus_tag="PLY83_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV28994.1"
                     /translation="MIHEHERVVRMVAELQNFLFSMGASEIRSEIRETPEAFLVSIKG
                     NTRGNICRKVEKLRKLLSLARRADMEEELWSLAGSVDFDTELSLVGMMSDRAEVRCDG
                     DAGVVEVELVRRK"
     gene            6401..6955
                     /gene="ilvN"
                     /locus_tag="PLY83_02785"
     CDS             6401..6955
                     /gene="ilvN"
                     /locus_tag="PLY83_02785"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOV28995.1"
                     /translation="MRRTLSVISEDNPGVLTRLAGLIYRRGYNVESLSVGRTDVPGFS
                     RFTVVVNGDDAVIDQVAKQLDKLIEVVSVRRLSPGNSVERWLLLMKVRAPIHMRHQVL
                     QTAEIFRCRVVDVGAEAVVLELTGDKGKVEACVEALRPYGILEMTSSGSVAMERAGFA
                     EARDEREGNAYSPADSLSSVAFTF"
     gene            6981..7991
                     /gene="ilvC"
                     /locus_tag="PLY83_02790"
     CDS             6981..7991
                     /gene="ilvC"
                     /locus_tag="PLY83_02790"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOV28996.1"
                     /translation="MAKVYYEKDAKPEVLKNRKVAVLGYGSQGHAHAQNLRDSGVTVV
                     VGLHAGSRSKAVAEKDGFIVKSVPEAVAEADIVMFLMPDHVQAKVFESEVAPAMKPGT
                     TLGFSHGFTIHYGQIVPPKTADVFMIAPKSPGHLVRRMYTEGKGVPGLLAVYQDATGM
                     AKEIGLAYAAAIGCARAGVIETTFAEETETDLFGEQAVLCGGVTELVKAGFDTLVEAG
                     YQPEIAYFECLNELKLIVDMMFEGGLAWMRYSVSDTAKYGDCVAGARVVDSHTRETMK
                     DLLKAVQDGTFAKDWILENQCGRPRMRAWQARERCHLIERVGAELRDMMPWLEAKRPP
                     EA"
     gene            8004..9560
                     /locus_tag="PLY83_02795"
     CDS             8004..9560
                     /locus_tag="PLY83_02795"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOV28997.1"
                     /translation="MPEDWVRIFDTTLRDGEQSAGVNLNTAEKIQIAHQLAKLRVDII
                     EAGFPAASPGDMEAVSTIAREVRGPVIAGLARTRPGDIEAAAEAVRHAEKPRIHTFIA
                     TSPIHMEHKLKMTPDQVLEEVRRAVTQARLFVADVEFSAEDASRSEIDFLVKVYSLAI
                     ECGATTLNIPDTVGYAAPDEFGRFVKAILDGVKAPPEVIWSVHCHNDLGLAVANSLEA
                     VRAGVRQVECTVNGLGERAGNASMEEIVMALRTRPDRYGVASRLETSRLYSTSKLVSR
                     LTGVLVPPNKAIVGENAFAHEAGIHQHGILCNRATYEIMKPEDVGAPGTALVLGKHSG
                     RHAFLKRLEALGFELDDDQKEKAFRLFKDLCDRKKTVSDGDIEALVVDEVLAVVPERR
                     FELKEYSVQVAGRGRASASITLRDGTSEVTDAAAGNGPVDAAYSAIRRLVGISPELTS
                     YRIQATSERSDAMGEAVITLRLGDLKAQGRGASTDVIEASIKAYVSAVNRLYALAAAK
                     GEVLNGENAG"
     gene            9541..10833
                     /locus_tag="PLY83_02800"
     CDS             9541..10833
                     /locus_tag="PLY83_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOV28998.1"
                     /translation="MGRTLAEAIIASHTADPVEPGSICRVRVDFAFANDITAAPAIAE
                     FSRMGAEAVFDPARCAIVPDHFTPNKDIASARQVQVARGFARKQGMLFWEVGRAGVEH
                     AFLPEKGLILPGEVILGADSHTCTGGALGAFATGVGSTDLAAAWALGETWFRVPETIR
                     VVMDGKASPWVTGKDLILSLIGRIGVEGARYAALQFEGPASDALPMDDRFTVANMAVE
                     AGAKAGLFVPDRRTLEYAKRRAARPFEPLYPDPDATYSQTIRIDAASLEPVVALPHLP
                     SNVKTAGECGRIDLDQVFIGSCTNGRLRDLELAAAVLRGRKVHERVRLVVIPATAEVF
                     AEALKRGFIETFLEAGAAVSTASCGPCLGGHYGILAPGERCLSTSNRNFVGRMGHPES
                     EVLLCGPLVAAASAVTGRVTDPREVLGLEELPKGVDEE"
     gene            10830..11348
                     /locus_tag="PLY83_02805"
     CDS             10830..11348
                     /locus_tag="PLY83_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOV28999.1"
                     /translation="MSDRFSGRAWVYGDNVDTDVIIPARYLSTSDPAELAPHCLEDLD
                     PGFMKRMVQGDILVAGSNFGCGSSREHAPLAIKGAGVSCVIAASFARIFFRNALNIGL
                     PILECPEAVSGIRSGQRIEVDLAEGRILNRDTGQAFSAKPFPPFLRELVARGGLVPTV
                     KARLEEKRRSTH"
     gene            11345..12496
                     /locus_tag="PLY83_02810"
     CDS             11345..12496
                     /locus_tag="PLY83_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HOV29000.1"
                     /translation="MTEKEYRVAVIPGDGIGIEVTAEAKKVVSAASGIFGFSVDWTDY
                     PFGADHYLSTGEVLSESFLEEAKGFDALLLGAIGDPRVKPGILERGILLTLRFAFDQY
                     VNLRPAKAFPKVPLPVDPGGARLDTVVVRENTEDFYMGIGGVSLEGRVEFPVACHRAA
                     YALDGKVSAETDPPFPMAVQLGIATKPGIERIARYAGLLARKRGEDRVTLVTKSNALP
                     QIYGFWEEVAEAVFASEFPGLVLEKANVDALCYHLVRKPAAYGVLLCPNMFGDIVSDL
                     LAGLSGGLGMAAGADIGPGLSMFEPVHGSAPDIAGQGKANPLAAILTAALMLDHLGET
                     EAARAVEKAVEVFLAESRPEDLPVELGGRASTEEVGLGVIRSLEGISKE"
     gene            12510..14189
                     /gene="ilvB"
                     /locus_tag="PLY83_02815"
     CDS             12510..14189
                     /gene="ilvB"
                     /locus_tag="PLY83_02815"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOV29001.1"
                     /translation="METLSGSEMVIRSLQAEGVEVVFGLPGGTVIPLYDAFHDVRKPL
                     HVLVRHEQAAAHAADGYARVSGRPGVCLATSGPGATNLITGLATAWADSVPLVAVTGQ
                     AATAVIGTDAFQEADMIGMTLPILKHSFPVREVARLPFALKGAFTIASTRRPGPVLVD
                     IPVDVQKARGPFAYPERVFFPGYRPEVPERLDDLPEAMAALKEAQRPLVLAGGGANDP
                     ESSRMLVELAEACGLPVAASFRGKGAFPENHPLFLGLCGMHGTLAANRAAREADFILA
                     VGTRFSERTTGRADRFAPGSKIVHVDIDPAEIGKNVPSRWSLVGTSAGVLRKILEALE
                     GWDKTPVSWRETVSGWKEEDKARLEMESLGFSPRRILKAIRAAMGDRALVTTDVGQHQ
                     MWAAQHFDSCGPRSFISSGGLGTMGFGLPAAIGAAFAAPERPLACITGDGSFLMNIQE
                     LDTCARYGIPVKIFLFNNGSLGMVRQWQELFYRERYAHTVTDGLVDFARIAEAFGVPG
                     IQVSTEKEIGAAIEKALDTDGPALVEFRIAMEEKVFPMIPPGAGMDEMIEG"
     gene            14263..>14750
                     /locus_tag="PLY83_02820"
     CDS             14263..>14750
                     /locus_tag="PLY83_02820"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOV29002.1"
                     /translation="MPDFEAREQGKRTTKGFTLVELLIALVVLGILAGALLLVSSAGA
                     DKAQAMRVVSDLRNLKAAAVQYNASSGSWPSSVTDLSGYLDGPLECSGPVCYEVASSE
                     AGNFVGFKADLARVSSGVKDRLKGMAETVSLYSDTALANLYAGQAEAVYPVGYAGSGA
                     MAA"
     assembly_gap    14751..14850
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14851..16273)
                     /locus_tag="PLY83_02825"
     CDS             complement(<14851..16273)
                     /locus_tag="PLY83_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOV29003.1"
                     /translation="MNFGFIVLWAGYALLLVFLARWARGKDHLLPGRVGSAVQALAYV
                     ATYVSAVALVGFGGLCHRYGLQMLLIAAGNIWFGTWFVYRFLAWPTRLWQRRLNAKTP
                     GALLGAAIGSRAFRLFAGLLTGGLLVVYASAVFKGAAMMLSGVTPLPFSASLWILVLL
                     VALSVTWGGLRGVLYTEALQGGIMLAGVALLLFAVFRAVGGLSTGLAALSALPPTELA
                     NRGFLRLSEGPQGLFVLTLAAVTSIGVWAQPQLIQRHFALSSRDEARRAAPVAMLTIA
                     VVVGGTYLAGGLSRLLITPEEAASPDAVIPLLVSRLLSLPGQQLFAMAIVSASLSTAS
                     ALLHIAAGSLGHDLTGRRLEGAAWKTATAACAIASGLFAAKSGTIIALLCTTSWSLIA
                     SAILVPYLALLAGKADRLATWASSLGGLAAASLWYAAGFAPTAKGLLGVQVPPAFSGI
                     HPFFVGIAFSTGLYLLGLLVSQWS"
     gene            complement(16570..>17406)
                     /gene="proC"
                     /locus_tag="PLY83_02830"
     CDS             complement(16570..>17406)
                     /gene="proC"
                     /locus_tag="PLY83_02830"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOV29004.1"
                     /translation="ATRRNLAKARALEGLGVRLTGDNREAAAESDIILLAVKPLQVVP
                     VLQEIAPFTDGKTVISFAAAISLDIMNRAAPGSRFIRAMTNTAVEIRKGYTVYACSST
                     VSEADRRLAEEILGSVGEVQAVDESHLDVLTAMAGSGPAYLYTVVESLIYGALRMGLP
                     RDLALRSAAHTLIGASELLLASGKHPAELRDSVVTPGGVTIEGLYELEEGRVRTAFMK
                     AVCAASTQARALAKDARIQAEKAITPFFASGQEEIRESALDKIVEPLYDGSNFPKGEA
                     SN"
     assembly_gap    17407..17506
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(17643..18812)
                     /locus_tag="PLY83_02835"
     CDS             complement(17643..18812)
                     /locus_tag="PLY83_02835"
                     /inference="COORDINATES: protein motif:HMM:NF014298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOV29005.1"
                     /translation="MNVILFSPHFPPNFMNFAIALRRRGATVLGITDQPPEAMNPALR
                     EALADVIRLDDLHRYDQAAAAVAAFRDRFGPIDRLESHNEHWLDSDARLRADFGIPGL
                     KPEDLAPMRRKSRMKEVFSRAGLSVARGRVVHKLEEALAMVAEVGYPVVIKPDQGVGA
                     TGTWKIVDRDGMERFFAQKPQVDMFLEEFITGSIVTFDGLADREGKIVFCGSLHYADG
                     MMEMVNDRLDVFLCTQRELPEDLLLAGTKAVGAFGLRERFFHVEFFRTAAEGRLIALE
                     VNVRPPGGLILDMYNYSSDIDLFAEWAEMLTNPAYVPLSYERKYHCTFLGRRDSRSYA
                     HDFNAILQAYGKELVHHERVPTVFSPAMGDTCFLFRSPDLDRIREIAAFVLKPGT"
     gene            complement(18858..19595)
                     /locus_tag="PLY83_02840"
     CDS             complement(18858..19595)
                     /locus_tag="PLY83_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOV29006.1"
                     /translation="MQTDGAAWFSPSLGQPMEMKVYGYGNQPVLVFPTSGGRFFEYED
                     FGMIQALRTFLDGGRLQLFTVDSVDRQSWLNGQASTAEKVARHEAYERYILEEVLPFI
                     ESRCPGGKRLIVTGCSLGAFHAANLFFRHPEPFDTVIGLSGIYGLSYSVGEDLSNGAY
                     FHSPLHYLQGLEDPWYLNRYRERRIVLCAGQGAWEEESARETIAMKNLLEAKGVPAWV
                     DLWGTDVSHDWPWWQRQLPYFLERLGF"
     gene            19685..20482
                     /locus_tag="PLY83_02845"
     CDS             19685..20482
                     /locus_tag="PLY83_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29007.1"
                     /translation="MNVKNLLLLVDPQNDFCDSRGALFVPGADEDARRTARMISRISS
                     LLDDIVVTLDCHHVLDVAHPLMWVDALGNPPPPFTVITLEDVKAGTWRPVRPEWEDRL
                     CRYLQDLEALRRYRLTIWPEHCLVGSWGFGIVPCVFDAVTKWERQQKKTATLILKGES
                     PWTEHYSAVKAEVPDPEDPRTGINHSLLERIRAADCVAVAGEALSHCVASTIRDLVEP
                     LGGDLSRLVLIRDATSSVPGFEPVGEAFLEEMAPLGLRLESSERFLA"
     gene            complement(20499..21773)
                     /locus_tag="PLY83_02850"
     CDS             complement(20499..21773)
                     /locus_tag="PLY83_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOV29008.1"
                     /translation="MRRTNRKTDTQGTERLPATVNRLWNRVPAYRAKMEQAGITPSDI
                     RGVEDLCALPFTTKQDLRDAYPLGLLACGREELLRVHASSGTTGKMTIVAYTKNDLEA
                     WKDCMANCLAIAGVTPRDTLQVMFGYGLFTGGLGFHYGGEHLGATVIPSGTGFTERQV
                     QLMQDLGTTVIACTPSYAVHLSEVLASRRKDQPGSGHSLKAAILGAEPWSEALRQTVQ
                     EGLGVPALDIYGLSEVMGPGVAIECPCQNGLHLNEDHFLAEIVDPVTGERLPEGQEGE
                     LVITTLTKEALPLARYRTRDLTCILPGKCSCGRGGIRIARIRGRSDDMLIIKGVNVFP
                     TQVEAALGRVEGLSMNYTLEVTEKNYVKDLSVSVESASGLGELEMERLERQAAKILHE
                     ALGLRVGVKVLLPGSLERFEGKARRVRVTSGA"
     gene            complement(22009..>23270)
                     /locus_tag="PLY83_02855"
     CDS             complement(22009..>23270)
                     /locus_tag="PLY83_02855"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOV29009.1"
                     /translation="LPVVLVQDSRKAAATAAAAVHGFPAETLSLAAVTGTNGKSTTAW
                     ITRSILRAGGISCGLLGTIVYDDGKREEPADRTTPDGPSVQRWLARIAANGGQACVME
                     ASSHGLDLHRLECCRFDALAFTNLTPEHLDFHGDMESYFQAKRRLFTDHARPEARFVL
                     NVDDPWGLRLAELFPDRALPYGIGQGTGLRGQILAMGLDGTDLEISVPGEKTPIRVRS
                     PLVGLHNVSNVLASIGIALSLGMGQEAIRYGIENLPPVPGRLQRFRFENGVTCFIDYA
                     HTPDGLEKALKAVGADCRGSVHAVFGLGGERTVENRPLMGRVAAALADTVVLTMDNPR
                     SEDPRTIARQIEEGLQGARLRSHSVILDRKEAVHAALDSAVSGDVVLLAGKGPETTMI
                     LADGPVPYNDEESVREWSRTRGIPILS"
BASE COUNT         4291 a         6968 c         7580 g         4231 t
ORIGIN      
        1 aatctttcca ggtctcttgc cagggtcccg gcgctgcagg aaagataggc gatccgtgcc
       61 ggcctgcagc gtgtcgcgag ccactccgtg atttccggct caaggcctgt cctgggcggg
      121 ttggcgtaga cgaggcggaa cgcttcgggg acctgctgga tccacgcttc cgcctgggga
      181 atgcgcaggg cgcagcgccc cctcaaaagg tccgcttcgg gagcgttgag acgggcacag
      241 tcgagggccg ctttggaaag ctcgatcccc agggtcctgg ctccccgggc tgaccatcgc
      301 gcaagcgttg ccccggctcc gcagtaaagg tcgatcaccg acgtgcccgg tcccggggaa
      361 aggaaggcct ctgcttcgtc aagggacctt gcgtaaagcg aaaggatgac ctgctgaaac
      421 ccctgcggcc cgtaaaacaa gcccatcggg ttgcgtgaaa ggggttttcc ccagaggagg
      481 cgcatggggc ccgcaccgaa gaggtgcctg ccggcgcagg ggaaaagatg gacccagagt
      541 ccctccaggc ctgtaagggc cagctcggcc tccttgccgg ccagccagcc gtcaccgggg
      601 tcgttttgcg ttttcaggat cagcgttgcc tgggcgccga cctgggagta aatggacagg
      661 gggaacaccc ctgccggggg gagggccttt gaaagccagg ccagggccga tctcacccga
      721 atgctctgga cggggcagtt ttccagcccg acaaggcggt cccggacctc catgccgaat
      781 ctccagcctt cttgcggatc ccaccgggcc ttcagggaca ccttgtcccg gtagccccag
      841 cgctctggac ctgcaggggc gacgatccgg cgaaggacgc ttgcccacgg ggcgagccga
      901 tcggccagcc aggcctgttt ctgggcaagg ctttcggctt cgtccagttc gcggtggcta
      961 cagcccctgc actctgcggg acacccttcc cggttgggtt tcggcacggc gggatcctcc
     1021 tctcttaaaa acaataatac tgtagtgaaa tgtagtcagg caaggcaatt catggtaaag
     1081 taactctaga gtctgtcctc ccaggcgagg tgccggaccg tggccttgtt ttcttttctg
     1141 cgcgggtgct tacgcttttt ccctgcttca cccgccccct tccgtgaaag gcttcctagg
     1201 ggcctttccg gaaagggttt tttgtcctgc ccgcccgcgc tttccccctt cctggtcctt
     1261 ctgccgctcc tcttgtccgc ttcaccggct cgggctgatt ccgccggcct ttcgcccagg
     1321 ggattcagcc tgcccgtggt cctgggcctc gttcttgctg cggccctggg ttcctttttc
     1381 gccggcaggc agacggggct gcgcgggctt cccgcggacg agggggcgtc cagaggcgcc
     1441 gaagcttcgc aggcggcggt cctggaaaaa acggaccagg cggaagaacc tccacctcct
     1501 tcctcggaaa agaaggaggt accgggggaa aggacagaac tcctcctgga agcggaggaa
     1561 ctggccagga tgggtttttt ccgggtggac ctcgaaaccg ggtcggtcga ggcctcccct
     1621 ttcctgaaaa agctgttcgg ctttgatccc gacgaggctc tgacctttca ggactacgaa
     1681 agccgaatcc acccggacga ccgctccagg gtcagggccg cgagggaaca ggctttttct
     1741 gcgggagaag acctctacga cctccggtac cgggtttcgc ccggttcccg cgtcatccac
     1801 caggtttccg cccgggtcca cgtgatccga aacccgaacg gccgaccttc gggacttttc
     1861 ggcgtgatcc aggacctttc ggaacgggag gaaatggaaa accggcttcg agtcagccag
     1921 gagctgtttc gccagtacat ggaaaacgct ccgagcgccc tcatcatggc ctccccgcag
     1981 gggcgcatcc aggacgtaag cccttctgcg gaacggcttt tccgccttcc cagggaacag
     2041 cttctggaga tgacgctcct ggacctcttc ccccagaact acgtcgacga ggggcggaag
     2101 cgtcttcgcg agcttgcggc acgggggcac ctgagcgtcg aggtcccctt ccgcacgtcc
     2161 acgggaaagg caggatgggg ccggtgggac gcggtcagcc tttcaaaggg agcctacctg
     2221 gtcttcctga cggatttgac ccaggagcat gaagcccagg aggcgctgct gcacagcgag
     2281 gcggctttca agaggctcgc ggaaaacctc ccgacgctga ttttccgcgt tgacgaggat
     2341 tttcggttcg tctacctcaa cccgagcacg gaacggttct ttggcaaaac gtcggaagag
     2401 ctgcagtgca aaaccctcgc ggattccggg ctgccgagcg cttttctccg gcagtggacg
     2461 gaacgtctga aggacgtctt ccggacggga caggggatgc ggttcgaagt gaacctggac
     2521 acgggggcaa cggttcgcta tctccagacc gtgctggttc ccgagaaggc ggacctgggc
     2581 atcgtccgct ccgtgctggc ggtttccacc gacatcacag agatcaagcg gcaggaggcg
     2641 agaacccagc agcagaggga cttcctggat gccctcatcc ggaatgcggg cgaagggatc
     2701 gccgtgttcg actccaccgg ccgctttctt gtcttcaacc cggccatgga ggccatgacg
     2761 gggtggaaac tggaagaact ccagccgccg gcgatttcct attccttctc ccggggggaa
     2821 ggggccgcgg aggcggcctc ggggatcttg aaggccctcg agggggtggc gtcggaggaa
     2881 gagcgccttc tgccctgcag gggggacgtc ctgaaaacgg tcatggtctc gtccagcccc
     2941 ctttccggga accccgaagc cctgacgctg gtcctcgtcc gggacatcac cgaacagaag
     3001 cgggccaccg aggcgctggc ccgggagcgg gcctacctgg acatgctgtt cgagaacgcg
     3061 ccggacggca tggtcgtgac ggaccggttc ggccagatcc ttcggctgaa ccgccgtttc
     3121 tccgagatgt tcgggtacga acgccaagag gccctcgggc gccagatcga caccctggtg
     3181 gttcaagggg cttccctcca ggagggaaaa accctgacgc ggcaggtggc ttcgggaagg
     3241 acggtcagca tcgagacgga gcggatccgc aaggacggga cgacgttccc ctgccgggcg
     3301 atcgggatcc ctatccgtct cgaggggggc gacgtccacc tttacatcct ctaccacgac
     3361 ctgaccgaga tccgccagag ggaggccgag ctccggctgg cgaacgaggt cgtcaagagc
     3421 agccccgtca tccttttccg atgggggaac ggaacgggat cccccatcga gtacgtttcc
     3481 gagaacgtcc gcttgtgggg gtatgaaccg gaggaactgg tttcgggccg aaaactttac
     3541 tccgagctga tccacccgga ggaccgggag aaaaccctcc aggaggtccg ggacttcgtg
     3601 agccagggcc gggacgagtt ccagcaggag taccgggtcg tgaagaaaaa cggggaaacg
     3661 atctgggtca acgaccagac ccgggttgtg aaaacggccg aggggtccat cgccttctac
     3721 cagggggtcg tcctggacat caccgaaaag aagcggtttg aactcctcct cgaagagaac
     3781 taccagacca tgagacgggc ctgggacgaa acgatcggag tcctttccac caccgtcgag
     3841 atgagggacc cctacacggc cggccaccag aggcgcgtcg ccaggatcgc gggggccatc
     3901 gccaggaaga tgggggtccc ggagcagatc ctttttgccg tggaacgggc ctcccttctc
     3961 cacgacatcg gcaagatcga ggtcccggcc gagatcctca gcaagccggg aagcctgtcg
     4021 gcgatcgaga tgggcatcgt gaagcagcac gtggaagccg gctggaagat cctgaaggac
     4081 atcgatcttc cctggccgct ggcggagatc gtctaccagc accacgaatt tcttgacggg
     4141 agcggctatc cccgcgggct aaagggcgag gaaatctgcc tcgaggcgcg gatcatcacc
     4201 gtggcggaca tcgtcgaggc catggcttcc caccgcccct tccgtccgtc gcggggcctg
     4261 gaggccgctc tttccgaaat cgagcatcaa aggggcatca ccctggacga acgggcggtg
     4321 gacgcctgcc tggaattgtt ccggcaggag ggtttcactt tggggggcaa ctgacccgga
     4381 ggattccagg gaggagggtt ggcgatggaa gactcgggaa cgactcctgc aggaaacagg
     4441 cttcactacg ggatcctggt gtcgaccttc ctgtcgctgg tcctggccgg cttccttttt
     4501 gacaccccgt cagggatcgt gaaaggcctt ttgaagatcg tcacggatcc cgattacctc
     4561 atcacggact acatgggcat cgggggtgtc ggggccgctt tcgtgaacag cggcctggtt
     4621 tccctccttt gcgtcgcgct tctcggcttt ctcaaggtgc aggtcacggg catcaccttc
     4681 gccgcgatct ggacggtggc cgggttctcc tttttcggga aaaacctcct gaatgtctgg
     4741 ttcatcatcg ccggagtccg ggtttatgct ttgtaccgga aagagccctt cagccggttc
     4801 atctattctg cgctgttcgg gacagccctg gccccgctgg tgacccagat cctcttcggg
     4861 ttcaacgtct cccccgccct gaggatccct cttgccgcgc ttgccgggat atcggtggga
     4921 ttcatccttc ctcccctggg ggtcgcgatg atgcggtttc accagggatt caacctctac
     4981 aacatcggct ttgtttcagg gctggtgggt tcgatcgtcc tttcgatctt ccggtcctac
     5041 gggtttatcc cccaaagccg gcttgtctgg accaaggggc acgatctcca gcttgctccc
     5101 ctctttttcg cgttcttcct tgcccatttc gcgctgggct atttcctggg ggggagaaag
     5161 gtttccggcc tggctgcggt gatgaggtac cctgggcggg tcgtcaccga tttcatcctc
     5221 ctggaaggac tcggtccgac cctgatgaac atggggctgg ggggaatggc atccatcctg
     5281 tatcttcttg ccgtccgggg cgatatcaac ggcccgacac tggccgggat ttttaccgtg
     5341 gtcggctttt cagcctttgg aaagcacctg aggaacatgg cccccattgt ggccggagtg
     5401 gtcctggggg gcgccacgaa gatctgggga atgaacgatc cgccgatcca gcttgctgcc
     5461 cttttcggaa caacgctcgc tcccgtggcg ggagagttcg ggatcttctg ggggatcgtc
     5521 gcgggttttc tccactcctc gctggtgctg aacgtcgggg cgctccatgg ggggttcaat
     5581 ctttacaaca acggtttttc gggcgggatc gtcgcggccg tcctggttcc cgtcatcgaa
     5641 gccatccgga cagggaggga aaaatcttga ttcacgagca cgagcgggtt gtccgcatgg
     5701 ttgcggaact gcaaaacttt ctcttttcca tgggggcaag cgaaatccgt tcggaaattc
     5761 gggaaacccc ggaggccttc cttgtttcga tcaaggggaa tacccgggga aacatctgcc
     5821 ggaaggtgga aaaactccgg aagcttctca gcctggcgcg cagggcggac atggaggaag
     5881 aactctggtc cctggcaggt tcggtggatt tcgataccga actctccctg gtcgggatga
     5941 tgtccgatcg agcggaagtt cggtgtgacg gagacgccgg ggtggttgag gtcgaacttg
     6001 tgagaagaaa gtgaagaaac gggatttgtt taaaatcggt ttagttattt gaaaaatttg
     6061 cttttttagc gttgcaaaaa gagggcttga caagaatctg gaagcccttt atgattcccc
     6121 ccaatatcga ttaatcgggc ggcgaaagcc ccaggcccgc atcctgagac caaggaggga
     6181 agaaccgtga agaccagcag cgtttttagc ctgcagctcg tcctggtact ggcgctgatt
     6241 attggacacg ggttccctcc tggggcagcc ggtgcctaag cgccgcgcat agaaaaaccc
     6301 aggccgggac ccgcaaaagt gagcgggtcc cggccttttt ttattcatcc agaagttttc
     6361 aagagacaga gagaaatcgt ttcagggagg tgtcaatgcg atgaggagga cgttgagcgt
     6421 gatttccgag gacaacccgg gggttctgac ccggctggcg ggcttgatct atcggcgggg
     6481 gtacaacgtg gagagcctga gcgtagggcg gacggacgtt ccggggttct cgcgctttac
     6541 ggtggtcgtg aacggggatg acgcggtgat cgaccaggtt gcgaaacagc tggacaagct
     6601 gatcgaggtg gtttccgtcc gccggctttc ccccgggaac tccgtcgaac gctggctcct
     6661 cctgatgaag gtcagggcac ccattcacat gcgccaccag gttctgcaga cggccgagat
     6721 tttccgctgc cgcgtggtgg acgtcggagc cgaggcggtt gtcctcgagc tgaccggaga
     6781 caagggcaag gtggaggcct gcgtggaggc tcttcgtccc tacggaatcc tcgaaatgac
     6841 ctccagcggt tccgtcgcga tggaaagggc cggttttgcg gaggcccggg atgaacggga
     6901 aggaaatgcg tattctcccg cggacagcct ttcgtccgtg gcattcacat tctgatctca
     6961 ttgaagggag aggttgggaa atggcgaagg tgtactacga gaaggatgca aaaccggaag
     7021 tcctgaagaa ccggaaggtg gccgtcctgg gatacggcag ccaggggcac gctcacgccc
     7081 agaacctgag ggatagcggt gtcaccgtgg tcgtgggcct gcacgccggg agccgctcga
     7141 aagcggtcgc ggaaaaagac ggctttatcg tgaaaagcgt tccggaagcg gtggcggaag
     7201 ccgacatcgt catgttcctg atgcccgacc acgtgcaggc caaggtgttt gaatccgagg
     7261 ttgccccggc aatgaagccg gggacgaccc tgggcttttc ccatggcttc acgattcact
     7321 acggccagat tgtgccgccg aagaccgcgg atgtcttcat gatcgccccg aaaagtcccg
     7381 gacacctggt tcgccgcatg tacaccgagg gaaagggggt tcccgggctg ctggccgtct
     7441 accaggacgc caccgggatg gcgaaggaga tcgggttggc ctacgcggcc gccatcggat
     7501 gcgcccgggc gggggtgatc gaaacgacct tcgcggagga gacggaaacc gaccttttcg
     7561 gtgagcaggc cgttctctgc gggggcgtga ccgagctcgt gaaggccggg ttcgacacgc
     7621 tggtcgaggc gggctaccag ccggagatcg cctatttcga gtgtctcaac gagctcaagc
     7681 tcatcgtgga catgatgttc gagggcgggc tggcctggat gcgctattcc gtcagcgaca
     7741 ccgcgaaata cggggactgc gtggcgggag cccgggtcgt ggattcccat acccgcgaaa
     7801 ccatgaagga tcttctgaag gccgtccagg acggaacctt cgcgaaggac tggatcctgg
     7861 agaaccagtg cggaaggccc cgcatgcggg cctggcaggc cagggaacgc tgccacctca
     7921 tcgagcgggt tggagcggag cttcgggaca tgatgccgtg gctcgaggcc aaaagaccgc
     7981 ctgaagctta gggggaatcc gggatgccgg aggattgggt acgaattttc gacacgaccc
     8041 tcagggacgg ggaacaatcc gcaggggtca acctgaatac agcggaaaag atccagattg
     8101 cccaccagct ggcgaagctt cgggtggaca tcatcgaggc ggggttcccc gcggcttcgc
     8161 cgggggacat ggaggccgtc tccacgatcg cccgggaggt ccgggggccc gtcatcgcgg
     8221 gactggcccg cacccgtccg ggagacatcg aagcggcggc cgaagcggtc cggcatgcag
     8281 agaagccccg gatccacacg tttatcgcaa cgagcccgat ccacatggag cacaagctga
     8341 agatgacgcc ggatcaggtc ctggaagagg tccggagggc ggtcacccag gcccggttgt
     8401 tcgtagccga cgtggaattc tcagccgagg acgccagccg gagcgagatc gatttcctgg
     8461 tgaaggtcta ctcgctggcc atcgagtgcg gggccacgac gctgaacatc ccggacacgg
     8521 tgggatacgc cgctcccgat gagttcggac gcttcgtgaa ggcaatcctc gacggggtga
     8581 aagccccgcc ggaggtcatc tggtcggtgc attgccacaa cgacctgggc cttgcggtgg
     8641 cgaattccct ggaagccgtc cgggccggcg tgaggcaggt cgagtgcacg gtgaacggcc
     8701 ttggcgaaag agcgggcaac gcctccatgg aagagatcgt catggccctg cgaacgaggc
     8761 cggaccggta cggagtggcc tcgcgcctgg aaacgagccg gctctactcc acgagcaagc
     8821 tggtgtcgcg cctgaccggc gtgctggttc cgccgaacaa ggccatcgtg ggggagaacg
     8881 ccttcgccca cgaggccggg attcaccagc acgggatcct ctgcaaccgg gccacctacg
     8941 agatcatgaa gcctgaggac gtcggggctc cgggaacggc gctggttctg gggaaacact
     9001 ccgggcgcca tgccttcctg aagcgcctgg aagcccttgg atttgagctg gacgacgacc
     9061 agaaggagaa ggccttcagg ctcttcaagg acctctgtga ccgcaagaaa acggtttccg
     9121 acggggacat cgaggcgctt gtcgtggacg aagtcctggc ggttgttccc gagcgccgct
     9181 ttgagctgaa ggaatactcg gtccaggtgg ccgggagggg gcgggcctcg gcatccatca
     9241 cgctgcggga cggaaccagc gaggttaccg atgcggcggc gggaaacggg ccggtggacg
     9301 cagcctacag cgccatccgg cgtctcgtgg ggatttcccc cgagctgacc agctaccgga
     9361 tccaggcgac cagcgaacgt tcggacgcca tgggagaagc cgtcatcacg ctgaggcttg
     9421 gagatctgaa agcccaggga cgaggggcca gcacggacgt catcgaagcg agcatcaagg
     9481 cctacgtcag cgcggtcaac cggctctacg ccctggcggc ggcgaaaggg gaggtcctca
     9541 atggggagaa cgctggctga ggccatcatc gcttcccaca ccgccgaccc cgtggagccg
     9601 ggctccatct gccgggtccg cgtggatttt gcctttgcga acgacatcac ggccgcgccg
     9661 gccattgccg agttttcccg catgggggcg gaagcggtct tcgacccggc tcgatgcgca
     9721 atcgttccgg atcatttcac tccaaataaa gatatcgctt cggcacgaca ggtgcaggtt
     9781 gcccggggct tcgcccgaaa gcaggggatg ttgttctggg aagtgggccg ggcgggtgtc
     9841 gaacacgcct tccttcccga aaagggactg atcctcccag gcgaagtgat cctgggggcg
     9901 gacagccaca cctgcacggg cggggccctg ggagcctttg ccaccggggt tggttccacg
     9961 gacctcgccg ccgcctgggc cctcggggaa acctggtttc gggttcccga gacgatccgg
    10021 gtggtgatgg acggaaaggc ctccccctgg gtgaccggga aggacttgat cctctcgctg
    10081 atcggcagga tcggggtgga gggggcccgc tacgccgccc ttcagttcga ggggccggcc
    10141 agcgacgctt tgccgatgga cgaccgcttc acggtggcga acatggctgt cgaagcgggt
    10201 gccaaggccg gactgttcgt tcctgaccgg cggacccttg aatacgcgaa gcgcagggcg
    10261 gcccgtccct tcgaaccgtt gtaccccgac ccggacgcga cgtacagcca gacgatccgg
    10321 atcgacgctg cctcgctcga gccggtcgtg gcgctgccgc acctgccgtc gaacgtgaaa
    10381 acggcgggcg agtgcggccg aatcgacctg gaccaggttt tcatcggcag ctgcacgaac
    10441 gggcgcctgc gggacctcga gctggcggcg gctgtcctca gggggaggaa ggtccacgag
    10501 cgggtgcgcc tcgtggtcat ccccgccacg gccgaggtgt ttgccgaggc cctgaagcga
    10561 gggttcatcg agaccttcct cgaggcggga gcggccgtca gcaccgcgag ctgcgggccc
    10621 tgcctggggg gacattacgg catcctggct ccgggggagc gctgcctttc cacgagcaac
    10681 cggaatttcg tgggaaggat ggggcatccg gaaagcgagg tgctcctttg cggccccctc
    10741 gtggcggctg cctcggcggt caccgggcgt gtgacggacc cccgggaggt ccttggcctg
    10801 gaagaactcc cgaaaggagt cgatgaggaa tgagcgaccg gttttcagga agagcctggg
    10861 tttacgggga caacgtggac accgacgtga tcattcccgc ccggtacctt tccaccagcg
    10921 atccggccga acttgccccg cactgcctgg aggacctgga ccccgggttc atgaaacgga
    10981 tggttcaggg cgacatcctg gtcgccggga gcaatttcgg ctgcggatcg agcagggaac
    11041 atgctccgct ggccatcaag ggcgcggggg tttcctgcgt aatcgcagcc tcttttgccc
    11101 ggattttttt ccggaacgcc ttgaacatcg gccttccgat cctggaatgt cccgaggcgg
    11161 tttccgggat ccgctccggt cagagaatcg aggtggacct ggcagaaggg cggatcttga
    11221 accgggacac gggacaggcg ttttccgcca agcccttccc gccgtttcta agggagctgg
    11281 ttgcccgcgg gggacttgtt ccgaccgtga aagcccgtct tgaagagaaa aggaggagca
    11341 cccattgact gaaaaggagt atcgagtcgc cgtcatcccg ggtgacggga tcggcatcga
    11401 agtgaccgct gaggcaaaga aggtcgtttc ggccgcgtcc gggatcttcg ggttttccgt
    11461 ggattggacc gattatccct tcggagcgga ccattacctc tccacgggag aggttctttc
    11521 agagtcgttt ctcgaggagg cgaagggatt cgacgccctc ctcctggggg cgatcggaga
    11581 cccgagggtc aagccaggga tcctggaacg ggggatcctc ctgaccttgc gctttgcctt
    11641 tgaccagtac gtgaaccttc ggccagcgaa ggctttcccg aaagtccccc tcccggtgga
    11701 tcccgggggc gcgagacttg acaccgttgt cgtgagagag aacaccgaag acttctacat
    11761 ggggatcggc ggcgtttccc tcgagggtcg cgtggagttc ccggtcgctt gccatcgtgc
    11821 cgcctatgcg ctcgatggga aggtttcggc cgaaacggat ccgcccttcc cgatggccgt
    11881 ccagctgggc attgccacga agcctggaat cgagcggatc gcccgctatg ccgggctgct
    11941 ggcccgaaag cgcggtgaag accgggtgac cctcgtgacg aaatccaacg ctcttcccca
    12001 gatctacggg ttctgggaag aggtggcgga ggcggttttc gcatcggagt tccccggcct
    12061 ggtcctggaa aaggccaacg tggacgccct ttgctaccac ctggttcgga aacccgctgc
    12121 ctatggggtg ctgctgtgcc ccaacatgtt cggggatatc gtgagcgacc tgctggcagg
    12181 cctttcggga gggctcggca tggcggccgg tgcggacatc ggcccgggcc tttccatgtt
    12241 cgaacccgtc cacggttccg ccccggacat cgccggccag ggaaaggcca atcccctggc
    12301 ggccatcctg acggccgccc tgatgctgga ccacctggga gagaccgagg cggcaagggc
    12361 ggtggaaaaa gccgttgagg tcttcctggc cgaatcgcgc ccggaggacc tgccggtcga
    12421 gctcggagga cgggcttcca cggaagaggt ggggctgggc gtgatccgtt cgctcgaggg
    12481 aatctcgaag gaataaggag gtcgttccca tggaaaccct ttccggttcc gagatggtga
    12541 tccgttccct ccaggccgag ggagtcgagg tggttttcgg actgccggga gggacggtga
    12601 ttccccttta cgacgcgttc catgatgttc ggaaacccct ccatgtcctg gttcgccatg
    12661 aacaggcggc cgcccatgcg gcagacggct atgcccgggt ttccggacgt cccggcgttt
    12721 gcctggccac ttccggtcca ggggccacga acctcatcac gggcctggcg accgcctggg
    12781 ccgactcggt tccgctcgtg gccgtcaccg gacaggccgc cacggccgtc atcgggacgg
    12841 acgctttcca ggaagccgac atgatcggca tgaccctgcc catcctgaaa cacagctttc
    12901 ccgttcggga ggtggcccgc cttccctttg cgctgaaggg agccttcacg atcgcctcga
    12961 cgaggcgccc gggcccggtt ctcgtggaca ttcccgttga cgtccagaag gcccgggggc
    13021 ccttcgccta tcccgagaga gtcttcttcc caggctaccg gccggaggtg ccggaacgcc
    13081 tcgacgatct gccggaagcg atggctgccc tgaaggaggc ccaaaggccg ctcgttctcg
    13141 ccggcggagg ggcaaacgac ccggagtcct cccgtatgct ggtggagctt gcggaggcct
    13201 gcggccttcc cgttgccgcc tcgttcaggg gcaagggggc cttcccggag aaccatcccc
    13261 tctttctcgg cttgtgcggg atgcatggaa ctcttgccgc caatcgggcg gcgcgcgagg
    13321 ccgacttcat cctggccgtg ggaacccgct tcagcgagcg gaccaccggt cgcgccgacc
    13381 gttttgcccc tgggagcaaa atcgtccacg tggacatcga tccggcggaa atcgggaaga
    13441 acgtgccgtc ccgatggtcc ctcgtgggga cctcggcagg agtcctgcgg aagatcctcg
    13501 aggcgctcga gggctgggat aagaccccgg tgtcctggag ggagaccgtt tccggctgga
    13561 aagaagaaga taaggcccgc ctggagatgg aatccctcgg tttttcgccg cggcggatcc
    13621 tgaaagccat ccgggcggcg atgggagacc gggcccttgt aacgaccgat gtcgggcagc
    13681 accagatgtg ggccgcccag cattttgatt cctgcggccc caggagcttc atttcctccg
    13741 gcgggctggg aaccatgggc ttcggcctgc cggcagccat cggcgcggcg ttcgcggctc
    13801 cagagcgtcc ccttgcctgc atcacgggag acggcagctt cctgatgaac atccaggaac
    13861 tcgacacctg cgcccggtac ggcatcccgg tgaaaatctt cctgtttaat aacggctccc
    13921 tcggaatggt gcgccagtgg caagaactgt tctacaggga gcgctacgcg cacacggtga
    13981 cggacgggct ggtggatttc gcccgcatcg ccgaggcctt cggagttcct gggatccagg
    14041 tttccaccga aaaggagatc ggcgcggcga tcgaaaaagc cctggacacg gacgggccgg
    14101 cgctggtgga attccgtatc gccatggagg aaaaggtttt tcccatgatc ccgccggggg
    14161 ccgggatgga tgagatgatc gaaggctgag ttggtggacc cggtttaacc tgctatacta
    14221 aaaagcgttt aaaaaacgcc caaaatggcg gggtgacagg ggatgccaga ttttgaagcc
    14281 agagaacaag ggaaacgaac gaccaaaggt tttacgctgg tggagctgct gatcgccctg
    14341 gttgtcctgg ggatcctggc cggggcgctg ctgctggtga gctcggcagg ggcggacaag
    14401 gcgcaggcga tgcgggtggt gagcgacctg cggaacctga aggcggcggc ggtgcaatac
    14461 aacgcctctt cgggatcctg gccctcttcc gtgacggacc tttccggcta cctggacgga
    14521 ccgctggaat gttcggggcc ggtctgctac gaggtggcct caagcgaagc cgggaacttc
    14581 gttggattca aggcggacct cgcgagggtc tcctcggggg tgaaggaccg gctgaagggg
    14641 atggcggaga cggtttccct ctattccgac acggccctgg caaacctcta cgcggggcaa
    14701 gccgaggcgg tctacccggt gggctacgcc ggaagcgggg ccatggctgc nnnnnnnnnn
    14761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cggaccactg gctcaccagg aggcccagga
    14881 ggtagaggcc cgtcgagaag gcgatcccca cgaagaaggg gtgaatcccc gagaaagcgg
    14941 gcggaacctg aaccccgaga agccctttcg cggtcggggc gaatcccgcc gcgtaccaga
    15001 ggcttgcggc ggcgagtcca ccgagggaag aggcccatgt ggccagccgg tctgccttgc
    15061 ctgccagaag ggccaggtag ggaacgagga tggcggaggc gatgaggctc cagctggtcg
    15121 tgcaaaggag ggcgatgatc gtcccgcttt ttgccgcgaa gagcccggaa gcgatagcgc
    15181 aggccgcggt ggccgttttc caggcagctc cctcgaggcg gcggcctgtc aggtcgtgtc
    15241 ccaggctgcc ggccgcgatg tgaagaaggg ccgatgctgt tgagagagac gccgagacga
    15301 tggccatggc gaagagctgt tgaccgggga gagaaagaag ccttgagaca agaagcggga
    15361 tgacggcgtc aggtgaagcg gcttcctccg gagtgatcaa aagccgggag aggcctccgg
    15421 ccaggtaggt gccgccaacg accaccgcga tggtcagcat ggccacggga gcggccctcc
    15481 tggcctcgtc gcgggacgag agggcgaaat gccgctggat cagctggggc tgagcccaga
    15541 ccccgatgga ggtcaccgcg gccagggtca agacgaaaag cccctggggc ccctcgctca
    15601 gcctcaagaa tccccggttg gccagttcgg tgggcggcag ggcggaaagg gccgcaagcc
    15661 cggtggacag gccgcccacg gccctgaaga ccgcgaagag gagaagggcc acgcccgcga
    15721 gcatgatgcc cccctggagg gcttcggtgt agaggactcc ccgaaggccg ccccaggtga
    15781 cgctcagcgc gacaaggaga accaggatcc agagggaggc agaaaacggc agcggtgtaa
    15841 ctcccgagag catcatggct gcgcccttga agacggccga ggcgtagacg accaggagcc
    15901 ccccggtcaa aaggccggcg aagagacgga aagcccttga cccgatggcg gcacccagca
    15961 gagccccggg ggtcttcgcg ttcaggcgcc tctgccagag gcgcgtcggc caggccagga
    16021 aacggtagac gaaccaggtt ccgaaccaga tgttcccggc agcgatcagc agcatctgga
    16081 ggccgtagcg gtggcagagt cccccgaagc ccaccagggc cacggcggaa acgtaggtcg
    16141 ccacgtaggc caacgcctgg acggctgagc cgacccgccc gggaagcagg tgatcctttc
    16201 ccctggccca tcgggcgagg aaaaccagaa gaagcgcata tcctgcccag agaacgataa
    16261 aaccgaagtt catcttcgaa tccccctttg cctggcgagt gtccagcaag accagaaaac
    16321 gcttgcgatc atggaagcca ctgccgttcc aacccacaga tcgaaccctg ccatcgcgat
    16381 cacctccaaa aaataaggcc gccagccctg ggggctggcg gccttgaacc ttctcacggg
    16441 tttcgaatca ggcggtgaga ggacaggacc gggcacctcc ggggcgatgc tgcaccggag
    16501 cgtaagcata gagatacgcc tggccctttc ctgttccgat cgaacaattc ttgttgatct
    16561 gccgattcat caattggaag cctcaccttt tggaaaattt gagccatcat acaggggctc
    16621 cacgatcttg tcaagagcgg actcccgaat ctcttcctgc cctgaagcga agaaaggcgt
    16681 gatggccttc tcggcctgga tgcgggcgtc cttggccagg gcccgggcct gggtcgaagc
    16741 ggcgcagacg gctttcatga aagcggttcg gacgcgccct tcttccaact cgtacagtcc
    16801 ctcgatcgtg acaccgccgg gggtgaccac gctgtcgcga agctccgcgg gatgtttgcc
    16861 cgaggccaga agcagttcgg aggccccgat gagggtatgg gcagccgacc ggagggccag
    16921 atccctgggg agtcccatcc ggagagcgcc gtagatcagc gattcgacca ccgtgtagag
    16981 ataggcgggc ccgctcccgg ccatggcggt caggacgtcc aggtgggatt cgtcgacggc
    17041 ctggacttcc ccgacagaac cgaggatttc ttccgccagc ctccggtccg cctcgctcac
    17101 cgtgcttgag caggcataga cggtatagcc cttgcgaatt tcaacggcgg tgttcgtcat
    17161 cgcccggatg aaccggctcc cgggagcggc ccggttcatg atgtccaggg aaatggcggc
    17221 ggcgaacgag atgacggtct tcccgtccgt gaagggggcg atttcctgga gcaccgggac
    17281 gacctggaga ggtttgaccg caaggaggat gatgtccgat tctgccgcag cttcccggtt
    17341 gtcgccggtc aggcgaactc caagcccttc gagggcacgg gccttggcca ggtttctccg
    17401 cgtggcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncggg gcacgggtag
    17521 cacgggaggg ccatgtcagg gcgccagttc aaggacatgc ggttcttcct ttcctgaatc
    17581 ggtaagaatt ttgcgaaagg atactcttgt ttcactcctt cgtcaagttc gtggaaaaag
    17641 gctcatgtcc ccggtttcag gacgaatgcc gcaatttccc ggattctgtc gaggtccggc
    17701 gacctgaaca gaaagcaggt gtctcccatg gccgggctga agaccgtcgg aacccgctca
    17761 tggtggacca gctccttccc gtaggcctgc aggatggcat tgaagtcgtg tgcataggat
    17821 cggctgtcgc gcctgcccag gaacgtgcag tggtatttcc gctcgtagga aagcgggaca
    17881 taggccgggt tggtcagcat ctcggcccat tccgcgaaaa gatcgatgtc cgaggagtag
    17941 ttgtacatgt cgagaatcag ccccccgggc ggccggacat tgacctccag ggcgatcagg
    18001 cggccttccg cagcggtcct gaagaactcc acgtggaaaa accgctctcg caggccgaaa
    18061 gccccgaccg ccttggtacc cgccaggagg aggtcctccg gcagttcccg ttgcgtgcag
    18121 aggaagacgt caagcctgtc gttgaccatc tccatcatgc cgtcggcata atgcagggag
    18181 ccgcagaaaa cgatctttcc ctcccggtcg gccaggccgt cgaaagtgac gatgctcccc
    18241 gtgatgaatt cctccaggaa catgtccacc tgcggctttt gagcgaaaaa gcgttccatt
    18301 ccgtcgcggt caacgatctt ccaggttccc gttgctccga ccccctggtc gggcttgatc
    18361 acgaccgggt agcccacttc ggcaaccatc gcaagggctt cttcgagctt gtgaacgacc
    18421 cttcctctcg caacggaaag accggcgcgg gaaaaaacct ccttcatccg ggactttcgg
    18481 cgcatgggcg ccaggtcctc cggcttcagg cccgggatgc cgaaatcggc cctcaggcgg
    18541 gcatcgctgt cgagccagtg ctcgttatgg gattccagcc tgtcgatggg gccaaaacgg
    18601 tcccggaaag cggcaacggc ggcggccgcc tggtcgtagc gatgaaggtc gtcgaggcgg
    18661 atcacatcgg caagagcctc cctgagcgcg gggttcatgg cttcgggggg ctggtcggta
    18721 atgccgagaa cggttgcgcc ccggcgacgg agagcaatgg caaaattcat gaaattgggg
    18781 gggaaatgag gagaaaacag gatcacgttc atcggggatt cctcctttca atcccgaagt
    18841 tcccagtctg caaaatctca aaagcccagc cgctcaagga agtagggaag ctgccgctgc
    18901 caccagggcc agtcgtggct gacgtccgtc ccccacaggt ccacccaggc ggggaccccc
    18961 tttgcttcca ggagattttt catggcgatc gtttccctgg cggactcctc ttcccaggct
    19021 ccctgccccg cgcagagcac aatccttctt tcccggtagc ggttcaaata ccacgggtct
    19081 tccagcccct gcagatagtg gaggggagag tggaaataag ccccgttgct gagatcctcc
    19141 ccgacgctgt agctgagccc gtagatcccg ctcagcccaa tcaccgtgtc gaagggttcc
    19201 gggtgccgga aaaagaggtt cgcggcatgg aaggccccaa gactgcagcc ggtgacgatc
    19261 aaccttttcc cgcccgggca tcggctttcg atgaagggca ggacttcctc gaggatgtag
    19321 cgctcatagg cttcgtggcg ggcgaccttc tccgcggttg aagcttgacc gttgagccag
    19381 gactgacggt cgacgctgtc cacggtgaaa agctggaggc gtcctccatc cagaaacgtg
    19441 cggagggcct ggatcatccc aaagtcctcg tattcgaaaa aacggccccc ggaagtcgga
    19501 aacacaagaa caggctggtt cccgtacccg tagaccttca tctccattgg ctggcccagc
    19561 gaagggctga accatgctgc accatccgtc tgcattggcg ggtcgctccc gtagtaaaat
    19621 gggtttatct attcaaagtt atagcacagt atcgataaaa gtttcagacc gacagaacgg
    19681 ggggatgaac gtgaaaaacc tgctgctgct tgtggatccc cagaacgact tctgtgattc
    19741 ccggggggcc ctctttgtcc cgggcgccga cgaggacgcc cgccgcacgg caaggatgat
    19801 cagccggatc tcttcccttc ttgacgacat cgtcgttacc ctcgactgcc accatgtgct
    19861 cgacgtcgcc catccgctca tgtgggtcga tgcccttgga aaccctcccc cgccttttac
    19921 cgtgatcacg cttgaggatg tcaaagcggg gacgtggaga ccggttcggc cagaatggga
    19981 ggatcgcctc tgccgatacc tgcaggacct ggaggctctc cggcgctacc ggttgaccat
    20041 ctggcccgag cattgcctgg tcggcagctg gggattcggg attgttccct gcgtgttcga
    20101 cgcggtgacg aaatgggagc ggcaacaaaa gaagacggcg accctcatcc tcaaggggga
    20161 aagcccatgg accgagcact attcggccgt gaaggcggag gtccctgacc cggaggatcc
    20221 ccgcaccggc atcaaccatt ccctcctgga gcgcatccgc gcggcggact gcgttgccgt
    20281 cgcgggggaa gccttgtccc actgcgtggc cagcacgatc cgggacctcg tcgaaccgct
    20341 gggcggagac ctgtcgaggc tggttctcat cagggacgcc acctcgagcg tgccggggtt
    20401 tgaaccggtc ggggaagcgt tcctggagga aatggcgccg ctgggcctga ggctggaatc
    20461 cagcgaacgg tttctcgcct gagaatccag cccgccgttc atgcgcccga ggttacccgg
    20521 acccgccggg ccttcccttc gaagcgctca aggctgccgg gcaggaggac tttcaccccg
    20581 acccgcagtc cgagtgcctc gtggagaatt ttagcggcct gcctctcgag ccgctccatt
    20641 tcgagttcgc ccaggcccga agcgctttcc acggacacgg acaggtcctt gacgtagttt
    20701 ttttccgtaa cctcgagggt gtagttcatg gaaagcccct caacccgccc gagagccgct
    20761 tccacctgcg tggggaacac gttgaccccc ttgatgatga gcatgtcgtc tgagcgcccg
    20821 cgaatgcgcg cgatgcggat tccccctctc ccgcaggagc attttcccgg aagaatgcag
    20881 gtcagatcgc gagtccggta ccgtgccagg gggagcgcct ccttggtcag cgtcgtgatg
    20941 acgagttctc cctcctggcc ttcgggtagc cgttctccgg tcaccggatc gacgatttcc
    21001 gccaggaagt gatcttcgtt caggtgaaga ccgttctggc aggggcactc gatggccacg
    21061 ccggggccca tgacttccga aagcccgtag atgtccaggg ccgggacccc cagtccttcc
    21121 tggaccgtct gccggagagc ctcgctccag ggctcggccc cgaggatcgc cgctttcagg
    21181 gaatggccgg aaccaggctg gtccttccgc cgggaggcaa gcacttccga aagatggacg
    21241 gcatacgaag gggtgcaggc gattacggtg gttccgagat cctgcatcag ctggacctgg
    21301 cgttcggtga agccggtccc ggaggggatc acggtggccc caaggtgctc ccccccgtaa
    21361 tggaatccaa ggccgcccgt gaagagcccg tagccgaaca tgacctgcag ggtgtcgcgg
    21421 ggcgtgaccc cggcgatggc aagacagttt gccatgcagt ccttccaggc ctcgaggtcg
    21481 tttttcgtgt aggccacgat ggtcatcttt ccggtcgtgc cggaggaggc atggacccga
    21541 agaagctctt cgcgcccgca cgccagcagg cccaaggggt atgcgtcacg caggtcctgc
    21601 ttcgtggtga agggcagggc gcacaggtct tcaacccccc ggatgtccga aggggtgatg
    21661 ccggcctgct ccatctttgc ccggtaggcc gggacacggt tccagaggcg gttcacggtt
    21721 gccgggagcc tttctgttcc ctgggtgtcg gttttcctgt tcgttctcct catgattctc
    21781 tcctcccttg ggataaaaaa agggccgggg cccctgttgg gaccccggcc tcgaaacggc
    21841 acgaacgcct aggcgtcggc cctccgaggg ggtcccacgc cggtaaaaaa gaaacggaca
    21901 gacatcttcg agttcgtcgg attcatcttt ttgtcctctc gcgatttcat aggggtaaat
    21961 tagtgagaac ttataccatg ccctcccggg attcgtcaag gggcccgttc aggaaaggat
    22021 cgggatcccc cgggtacggc tccattcgcg gacgctttct tcgtcgttgt agggaaccgg
    22081 tccgtccgcc aggatcatgg tcgtttccgg ccccttgcca gccagcagca cgacgtcccc
    22141 cgaaacggcc gaatccagcg ccgcatggac ggcttccttc cggtccagga tgaccgaatg
    22201 ggaacggagc cttgcgccct gcagtccctc ctcgatctgg cgggcgatgg tccgggggtc
    22261 ctcgctgcgg gggttgtcca tggtcagcac gacggtgtca gccagggcgg ccgcaacgcg
    22321 tcccatcagg ggacggtttt ccacggtcct ctccccgccg aggccgaaca cggcatgaac
    22381 ggatccgcga caatcggccc cgacggcttt cagggctttt tccagcccgt cgggggtatg
    22441 ggcgtaatcg ataaagcagg ttacgccgtt ctcaaagcga aatcgctgca gccttccggg
    22501 cacggggggg agattctcga ttccataccg gatggcttct tgacccatcc ccagggaaag
    22561 ggcgatccca atcgaggcca ggacgtttga aacgttgtga aggcccacaa ggggacttct
    22621 gacgcggatc ggggttttct cccccggcac ggagatctcc aggtccgtcc cgtccagtcc
    22681 catggcaagg atttgtcctc tcaggccggt cccttggccg attccatacg ggagagccct
    22741 gtccggaaag agttctgcga ggcgtagccc ccaggggtca tccacgttca ggacgaagcg
    22801 ggcctccggg cgggcatgat ccgtgaagag tcttctcttt gcctggaagt agctttccat
    22861 gtctccgtga aaatccaggt gctcgggagt caggttcgta aacgccaggg catcgaagcg
    22921 gcagcactca agccggtgta gatcgaggcc gtgcgaagag gcctccatga cgcaggcctg
    22981 tcccccgttc gccgcgatgc gcgccagcca gcgctgaacc gaagggccat ccggcgtggt
    23041 ccggtcggct ggctcttccc ttttcccgtc gtcgtagacg atggtcccta gaagtccgca
    23101 ggaaattccc cccgcgcgca ggatcgatct tgtgatccag gcggtggtgc tcttcccgtt
    23161 ggttccggtt acggcggcaa gagaaagcgt ttcggccggg aacccgtgga cagcggccgc
    23221 tgccgttgcc gcggccttcc tggagtcctg caccagcacc accggcaggg
//
LOCUS       DAOOVN010000015        22546 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_1566_length_22546_cov_15.883198, whole genome shotgun
            sequence.
ACCESSION   DAOOVN010000015 DAOOVN010000000
VERSION     DAOOVN010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 22546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22546
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1566_length_22546_cov_15.883198"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..191
                     /locus_tag="PLY83_02860"
     CDS             66..191
                     /locus_tag="PLY83_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29010.1"
                     /translation="MGETRPVVVAFGKEQDLCLVFQPAEGLGVDDAVPVNLEAGA"
     gene            complement(362..>1579)
                     /locus_tag="PLY83_02865"
     CDS             complement(362..>1579)
                     /locus_tag="PLY83_02865"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556155.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOV29011.1"
                     /translation="SQVFTVDGRPFRKGDWITIDGSRGLVIPGKVELVQPSFTEDIRS
                     LLEWADEFAALEVWANADTPEDAARARGFGAKGIGLCRTEHMFMAADRLPVMQEMIIA
                     STREERLVPLEKLQRMQREDFEGIFEAMAGLPVTIRLLDPPLHEFLPKEHELKKELDL
                     LASQGKESGPEADAVRLKLAKAAALHENNPMLGFRGCRLGIVYPEIYEMQLRAIFEAA
                     CALSQKGVKVIPDIMMPLVGTREEMNRLREMTERIAEEQMARFGCRVHYLVGTMIEVP
                     RAALVADQLAESAEFFSFGTNDLSQTTFGYSRDDAEGKFLAEYVRQGILPANPFHTLD
                     RDGVGQLMSIAVTKSRPVRPAIQIGICGEHGGEPNSIAFCHSLGLRYVSCSPFRVPVA
                     RLAAAHSALGLIR"
     assembly_gap    1580..1679
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1680..2797)
                     /locus_tag="PLY83_02870"
     CDS             complement(<1680..2797)
                     /locus_tag="PLY83_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOV29012.1"
                     /translation="MTGKGKKLLYDFSEGSAEMKALLGGKGANLAQMMRIGLPVPPGF
                     TLTTEACLLYMEDPSGTMEEIWPEVKKAMKSLEEKTGKNFGGPGTPLLVSVRSGAPVS
                     MPGMMDTILNLGLNDETCSALSDTSGDRRFALDSYRRFIQMFSDVVLGVPAEEFENVL
                     TLTRRKLKVQYDNEIPADAMEEVVSQFKKIVRKTTGKDFPSDPWIQLKAATEAVFRSW
                     NTPRAVTYRKINNIPDNLGTAVNIVAMVFGNLGDDCGTGVCFTRNPSTGEKGLFGEFL
                     INAQGEDVVAGIRTPEPIQKMGEKMPALYRELLETAEILERHYKDMQDIEFTVERGKL
                     YMLQTRNGKRTAKAAVQVAVDMANEGLIDRQTAVSRVSP"
     gene            complement(2827..3627)
                     /locus_tag="PLY83_02875"
     CDS             complement(2827..3627)
                     /locus_tag="PLY83_02875"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HOV29013.1"
                     /translation="MTVDVFVVSDFTGETAEHVARAAYSQFGADAARLSRFRYISTEE
                     KAEDVLQVAQEKNALIICTMVDHKLRKYFVERAAERKVEVIDILGPILGALENRLGRP
                     PLETPGLLRRMDEEYFRRVKAIEFSIRCDDGRSPDLLPEADLVIIGVSRTSKTPLSMY
                     LAHKGVMVANIPLVPEATPPDELFRVSPDKVIGLMVKPEHLVQIRRERLRVLGLDADV
                     STYAQWERVEEELGFARNIMRKIGCRCYDVTNRAIEETAQEILDQIRA"
     gene            complement(3666..5735)
                     /gene="glyS"
                     /locus_tag="PLY83_02880"
     CDS             complement(3666..5735)
                     /gene="glyS"
                     /locus_tag="PLY83_02880"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOV29014.1"
                     /translation="MTEKRDFLLEIGTEEIPARFMPWALEESRRLFAESLAEAHIDHE
                     GITTLGTPRRLAIQVKGLASKQEDRVEQYRGPLWTQAFDAAGNPTKAALGFARSKNVP
                     IDELKSTIVNGQEYVLAEVRHEGRETVALLPDICTGVIRRLVFPKNMYWDDPSIRYAR
                     PIRWIVALWGNAVLPFRFGSLESGRITRGHRFMGDRSIEIPSPKEYMDLLFANFVIVD
                     PAKRREKMLSAISAIEKQIGGKAELDPDLVEENLFLVEYPVPFSGSFDKDFLEIPEEV
                     LTTSMKHHQKYFPVRDRNGNLLPHFIGVSNNQATSMNVVREGNERVLRARLSDAGFFW
                     REDRKVPLSERVEELKAIVYQERLGSVYEKAMRVRSVSLKLAERVPGLEPHRAVIDRA
                     GILAKADLVTSMVYEFPELKGVMGREYALQNGEDPDVALAIYEQVLPRFSGDALPSSP
                     AGAVLGVVDRADSLVGCFKVGLVPTGSQDPYALRRAARNINEILWNLDLDIDLFPVFE
                     DLAEALDAPGETLEAVRDFFIQRLQIQLREKGFSHGLVSLGVQLMWNRPCQVQKILNG
                     YAAVLGQEWFRGLVTAAVRVKNILSKAGEFDAEVREGAFIEDEESFLYQAISNLEPQV
                     REALSRQAWNEVTTLLAKLEPSITRFFDKVLVMDENPAVRQNRLALLSRCQDLFMSVG
                     DLGLLKE"
     gene            complement(5751..6629)
                     /gene="glyQ"
                     /locus_tag="PLY83_02885"
     CDS             complement(5751..6629)
                     /gene="glyQ"
                     /locus_tag="PLY83_02885"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HOV29015.1"
                     /translation="MNFQEVIMRLERFWAEQGCVIQQPYDIEVGAGTMNPATTLRSLG
                     PEPWKVAYVEPSRRPTDGRYGENPNRLQHYYQYQVIIKPAPENIQEIYLDSLRALGID
                     PADHDIRFVEDDWESPTIGAWGLGWEVWLDGMEITQFTYFQQVGGVDMNPVPAEITYG
                     LERIAMFVQKIDNVYDLNWVGKVRYGDIHHRGEVEFSTYNFDLADTGMLFMLFNAYEA
                     EATRILDKGFVLPAYDYVLKCSHTFNLLDARNAISVTERTGYIGRVRAIASRCCQAFA
                     KQREEMGYPLLKKFSN"
     gene            complement(6649..7377)
                     /locus_tag="PLY83_02890"
     CDS             complement(6649..7377)
                     /locus_tag="PLY83_02890"
                     /inference="COORDINATES: protein motif:HMM:NF014607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO C-terminal
                     domain-containing protein"
                     /protein_id="HOV29016.1"
                     /translation="MDFLPSLPQGHYQQLGVVLRATISGERAKNLLLLLKEAGLVWAT
                     AAGAASGRSRLSGVTEPLVWGYFTLYRSPRRIYVREVAVREDFWSLKQNKEKLRAALE
                     ICADAARFSLPGHPPAELLPILFWTLKALEAGTEPAAVRLRFLYRWGRALGIVPSLDR
                     CSACGKPAPQGLLTLQGLLCTACAGPFPAKECLSLGEKELNRLKKAVMLSGKDFRKES
                     FSRDEIPLFGKVSTLLTRLIESSL"
     gene            complement(7382..8296)
                     /gene="era"
                     /locus_tag="PLY83_02895"
     CDS             complement(7382..8296)
                     /gene="era"
                     /locus_tag="PLY83_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOV29017.1"
                     /translation="MNPEGHRAGLVTLVGRPNVGKSSLVNALVREKVCIVSDRPQTTR
                     NRIRAIDSSERAQIVFVDTPGVYPAKNRLGEFLLEEAKASLESVDLILYVVEAGPYSP
                     EPEEQGLLELIGSLAVPKILVANKADRLHGEIPLQAVLKPYLDALIPVTMIAVSAKTG
                     FNIEALREKIISLLPEGPSLFPEDILMDHPERFLAQELIREQVFRQTRDEVPHGVAVE
                     IETFKTPFEYPERQKALIQATIYVERPSQKGILIGAGGKRLKEIGRLARLSLENRLGC
                     PVFLELWVKVLPDWRKSPQQLRRFGYGR"
     gene            complement(8286..8714)
                     /locus_tag="PLY83_02900"
     CDS             complement(8286..8714)
                     /locus_tag="PLY83_02900"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOV29018.1"
                     /translation="MSPRTWPSFPVDAETLLEAARQGRCRAYAPYSGFRVGAAVLTHD
                     GSVHIGCNVENASFGLTVCAERIALFSALSAGAGKPIAVAVAGKEGTPCFPCGACLQV
                     LAEFNGEMAIILEQGERPVVYGLAELLPQRFDLGRPFHES"
     gene            complement(8711..10006)
                     /locus_tag="PLY83_02905"
     CDS             complement(8711..10006)
                     /locus_tag="PLY83_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOV29019.1"
                     /translation="MEVDIPSLINHLLLLLLLLFLSALCSFSETAITSSGKGKLLALQ
                     EKHPKSRKTLGWLMSNVQSVLTVTLVLNNLVNIAATAVATALLIQLVGSQGLWLSVLV
                     MTSLIVVFSEILPKTVAVARPESLLLKVIAPLRFISRLMFPINAVILFIVRSIGKLFG
                     VDLSSQHPFVTREEIEQMVTIGGDTGALEEEERKMIHGVIAFEETRAHEIMVPRMDVV
                     SIPYNLTIQEAVKVFQEHGHSRLPIHKGSLDHVVGILYVKDLIPALVSGQVDMPVSKL
                     KREAMFVPETMKIAQLFGIMKGKRVHMAVVVDEYGGTAGILTLEDLLEEIVGEIQDEY
                     DEENPDIVKEREGTYLVKGHVNLEDLSETLEYPFESEDVFSVAGFILALAGRFPDEGQ
                     VLEFGPWNIEVLEVEDHRIKLLRLDRQQTGERREGAVGE"
     gene            complement(10043..10537)
                     /gene="ybeY"
                     /locus_tag="PLY83_02910"
     CDS             complement(10043..10537)
                     /gene="ybeY"
                     /locus_tag="PLY83_02910"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOV29020.1"
                     /translation="MKLAVQIDDEENLLEGFGISSPDYDALEETWQSYLEEKAPDLAL
                     SGMVCVSLKICSAETIKDLNRRYRFLDEPTDVLSFPMWEQGGEFSPPRGWSEIPLGDI
                     VICPEVVKLSSDAESRDVSYDFLLMLMHGFLHLLAWDHDTEEKESAMFSEQENLLRRY
                     LQKV"
     gene            complement(10515..12575)
                     /locus_tag="PLY83_02915"
     CDS             complement(10515..12575)
                     /locus_tag="PLY83_02915"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOV29021.1"
                     /translation="MNPTSRAGRPKPWALHRLREIDLSYWTLRAALLVVVFFLVLLGW
                     WLGESREGYRLGEPSPRTYLAVLSTRYIDEENTERLRQIAEQKIVSVMTRDTHAERRI
                     RDSLEQLSRWDATEGLSDSLRDLLSTLPAEKRIRMLHLAKDLGLSLLEVRDWDKDLQP
                     KLWDRIIRLPLSRPDQNTIFQILVEIFQEPVQVDEEATEKLKRETAGKIEPVVRMIYP
                     GDVIVSRGEMVTPQKFRLLEAQGYPKGDLPYRSLVFILFSVFVWSLWPTVYANRREIQ
                     VSRRDFVFIICLLSAGWTIQVMSAIFKADTLAFLPLTGWLYLTVPTSLAFHVSLGGAV
                     IGALLSYSGSMAQLILWALSGIAASITGYLVFRGSNTRLDLWKQQVLTGISAAVVATA
                     VRWWLGLPLESHVQGVYLLSVLGWATFTVALLPMLDTMFDILSPLRLMDLSHPSNPLL
                     KRLQMEAPGTYHHTLMVGTLCEAAADVLGLDSLLVKAGAYFHDIGKLKRPQFFAENQM
                     PGENLHDELKPSMSALVILSHVKDGLDLARRYNLPTRIRHFIAEHHGTTCLNYFYRKS
                     KARGEDLPREQFSYPGPKPSSKETALVMLADSVEAAVRAAGGSIRGLPELRETVEGVL
                     DSKMLDGQLELVDFTLKDLTLIKESFIQTLRSIYHKRDNTGTRSLFEPEQGGSEQGEI
                     SGSD"
     gene            complement(12544..13494)
                     /locus_tag="PLY83_02920"
     CDS             complement(12544..13494)
                     /locus_tag="PLY83_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOV29022.1"
                     /translation="MSADSEETLSRLLGPGDENLKLIERLLDVRVSARGLSVSVSGQE
                     PERVEKAEKLLIQLLEVCRRGHPVGSSEIRYAAEAVGERADFNLASLYEDVICLTTRG
                     KPVRARTEGQRIYLEAMKKNELVFAIGPAGTGKTYLAVAHAVAALKAGRFNRIILVRP
                     AVEAGEKLGFLPGDLMEKVEPYLRPLYDAFFDLLQPDRFLRYIDKSVIEIAPLAYMRG
                     RTLNESCIILDEAQNTTPEQMKMFLTRMGFGSQVIVTGDITQVDLPSGKESGLKIVEQ
                     VLGGIPGIAFIRFGHGDVVRHELVQRIVRAYESYEQGRQA"
     gene            complement(13560..13796)
                     /locus_tag="PLY83_02925"
     CDS             complement(13560..13796)
                     /locus_tag="PLY83_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOV29023.1"
                     /translation="MTATEQILKVIEEEIKPALQSHGGDIEFIRFDEPEGKLAVRLVG
                     ACGTCPFAQETLRVQVESVIKSIVPAVKSVIREN"
     gene            complement(13857..15314)
                     /locus_tag="PLY83_02930"
     CDS             complement(13857..15314)
                     /locus_tag="PLY83_02930"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HOV29024.1"
                     /translation="MENKPDRPNGSTEPCPENREQVPGKKVKIMETAFRDAHQSIMAT
                     RLRTEDMIPVAELMDEVGYGAMEIWGGATFDTCMRFLDEDPWDRLRELRKRFKKTKLQ
                     MLLRGQNLVGYRHYGDDAVREFVKRAIGNGLDIIRVFDALNDLRNMEIAADQVKREGA
                     HLQMALSYTISPVHTLENFADIAHQMQDMGADSICIKDMAGLMSPVDAFSLVKAIKER
                     TGLPVQVHSHYTSGMAAMAYLAAIEAGADVIDCAISPLAMGTSQPATETMIAALRGGP
                     YDTGMTLESLLPVALHFKEVKEKYSSLVMGIEGVDINVLIYQIPGGMYSNLVSQLKDQ
                     KALHRLDEVLKEIPRVRQEMGYPPLVTPTSQLVGTQATLNVLMGRWKVVPKEIRQYLL
                     GYYGRPPAEVDPEVRRQVIGEETPITCRPGEVLPPELEEAKKAIAPWILQPEDVLSYV
                     LFPAVAKDFLVRKFARATLRDVGLEKPVEEGVYPV"
     gene            complement(15351..16157)
                     /gene="mazG"
                     /locus_tag="PLY83_02935"
     CDS             complement(15351..16157)
                     /gene="mazG"
                     /locus_tag="PLY83_02935"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOV29025.1"
                     /translation="MEHRHDAPNGDFSRLVSIMKKLRGPEGCPWDREQDFKTLRRYII
                     EEAYELVEAIENEDDRAILEESGDLLLQVVFIAQIASETGSFSIRDVLQAICSKLVHR
                     HPHVFGDLNVADSEEVMRNWEAIKTVEKKKNGTDSSILSGIPRSMPSLLKAFRIQERA
                     AKFGFDWPKGDATPVIAKVREELAEIQEELSSEKGNRIGEEIGDLLFAAVNLSRHCGV
                     NPEEALQKASQKFADRFRLVERSVAESGRPWKEFTLDELDDFWEKAKQSC"
     gene            complement(16182..19271)
                     /gene="mfd"
                     /locus_tag="PLY83_02940"
     CDS             complement(16182..19271)
                     /gene="mfd"
                     /locus_tag="PLY83_02940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOV29026.1"
                     /translation="MPLDLIRKGDWGPWQNRDQALIAGSAARAWLLRDFPFPALVILP
                     DTRRQMEFFSDLKTLTEDNLALACLPEIPLTTSGLSNIGIRIQRGEELARWKTRLGIL
                     VCTPGALMGPCALAEGKVSLRLGDRIARASLLGWLVSSGYERVDTVWSPGQFVVRGSI
                     LDLFDPSIRSPLRVEFLDDRIESLRFFNPQTQRSTGPVDTVEISAVREGRSPSLEEFF
                     PENTRIFLFEPAETSSQADHYRWVWEGLKEEAGVEGLPEWDSVYLRLSRFPLLRIKAS
                     TERVSGKIGLAPVPSFKGRLQALDAALSEWQNLGLKVSVHTADRSLALRLTEKGIAVK
                     EAPLSSGFISPSENLVFVSDSDISGITALQPTEGVSLPPLEWSERLTIGEWVVHEDYG
                     IGRFVGIESVPSVQSEKQEYVVIEYAGGQRLLVPPFQLWKVTPYLAPRGAEPSADSLK
                     SGHWKKSLARVRRKVHEAALQLLQTYALREVSPGFAFPKDGPFSAQAEASFPFSETAD
                     QLRAIQEIKKDMEKPQPMDRLLVGDVGFGKTEVAFRAAVKAAESGKQVAVLVPTTLLA
                     QQHYETFLSRIGELPLRVEALSRFVPKRKQQEIVRSVAEGKVDILIGTHRLVQKDVRF
                     KDLGLVIVDEEHRFGVLHKEHSKKLKPDVDVLMMSATPIPRTLFLSLGGLRDISVIST
                     PPHQRHPVLTVVSPWREDLLLRGVLREKARGGQTFFVHNRIADIEARAGTLRTLFPSL
                     RIDIAHGQMGEGALEEAMLRFYSGETDVLVCTTIIESGLDIPRANTLVVDDAHELGLA
                     QLYQLRGRVGRREEQAFAFFFYPREFPLSREARERLEAIGELADPGSGYELAQRDLEI
                     RGGGQVVGTAQHGHVERVGYHLYCRMLEDQVSILRGKPLAGTRIEILAPLQIPPSYIP
                     QTGVRIALYRRLLREQDRNELFSLKAEVRDRFGPLPPAVEGIFGLALLRIEGDRLGID
                     SVQVSSKETTLKGTLAGIPALQRATGWKKGKNWAVGPGGFQGIASLVKLIDWQQFSH"
     gene            complement(19282..19839)
                     /gene="pth"
                     /locus_tag="PLY83_02945"
     CDS             complement(19282..19839)
                     /gene="pth"
                     /locus_tag="PLY83_02945"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOV29027.1"
                     /translation="MAGLGNPGLEYAWTRHNLGWLVADQVISRLHLKGPVNRFHGLLW
                     GPCRVGSDSLFVLKPLTYMNLSGKSVLAAVRELNLPPEECLIVVDDVALPFGTLRLRA
                     SGSAGGHNGIASILGALQTLHVPRLRCGIGRPVQAKGLADYVTDRFDSEEQGQLDSFL
                     EKAADAVMLWIRLGIDRAMNRVNAG"
     gene            complement(19854..20510)
                     /locus_tag="PLY83_02950"
     CDS             complement(19854..20510)
                     /locus_tag="PLY83_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOV29028.1"
                     /translation="MSELLQVTLEAREKSGKQEASKLRAEGYVPAVFYGRGYPDSIPV
                     KVKNRDILPALNSGHWETYRLEVTLPDGKTEMALMRDVQRDFLNGNLLHIDFYQLVSG
                     QKVQVKVPIEILNRDICPGVKAGGLLEQLFHEVSIEVLPREIPDVLSVDVKNLGLGEE
                     IRLRDLVLPESAELVDDGDLPVVIAAEPRAAEAAPAEESEQKEVEVLAKGKAKNQEEA
                     "
     gene            complement(20559..21527)
                     /locus_tag="PLY83_02955"
     CDS             complement(20559..21527)
                     /locus_tag="PLY83_02955"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOV29029.1"
                     /translation="MVTQLRQMKIFTGSAHPAFAQKVADTLGVPIASSKLFRFSDGEI
                     GLSIEESVRGADVYVVQPTSEPVNENLMELLIMIDALRRASAYRINVVTPYFGYARQD
                     RKTKAREPITSKLVANLIEKAGADRLVVADLHAGQIQGFFDRPVDHLTGIPLLASYFK
                     ENLKEAFDQQKIVVISPDIGGVVRARRFAVKLNADLAIVDKRRSHEVANFCEVMEIIG
                     DVRGKVAILVDDIVDTAGTMVNAARALLERGAERVYACATHGVLSGKAIENLRNSPIH
                     QLVLTDTIPLPEEKKLDNITVLSIAPLFAEAINRIHSDHSVSILFY"
     gene            complement(21512..>22546)
                     /gene="glmU"
                     /locus_tag="PLY83_02960"
     CDS             complement(21512..>22546)
                     /gene="glmU"
                     /locus_tag="PLY83_02960"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HOV29030.1"
                     /translation="GNAATVMTFRIENPFGYGRVIRDGDRCRIVEEKDASPDEHSVNE
                     VNSGIYVFSTVALLRVIDRLGNRNAQGEFYLPDVFPLLSESGLRVEPLLWEKATDLFG
                     INDPLQLAEANREMRGRILRKHLKNGIKMMDPQTTWIGPDVLLQPDVWIEADVQIWGK
                     TEIECGAVVGSHSILRNALVREKAHLVAFCCVTDSEIGPGAKVGPFAYIRDNSCLASG
                     AYAGKFVEVKNTFIGENSKVPHLSYLGDARVGKRTNIGAGTITCNYDGSRKNPTRIGD
                     DCFVGSDTMLVAPVVLNDGCFTAAGSTVTEDVPAGALAVGRARQKNIEGWALRKKAVS
                     RKEEEGWSRS"
BASE COUNT         4429 a         6595 c         6540 g         4882 t
ORIGIN      
        1 gttagatcct cgttgaggcg gaggcaacgg gcgatcgtcc gggcgatctg ggagacttcc
       61 agcgtatggg tgagacgcgt ccggtagtgg tcgccttcgg gaaggagcag gacctgtgtc
      121 ttgtgtttca gcctgcggaa ggacttggag tggacgatgc ggtcccggtc aacctggaag
      181 caggtgcgta gcgggcaggg aggctcttcc cgctgccggc ctttcgtgcg agcgctgagg
      241 caggcctctg gggagaggat cgacttttcc agttcttccc atcgaatccg gctgttgatg
      301 gcaatccctc ctcgggatca ggggaagccg gaccttccgg ccccccctga gatcatggat
      361 ttcagcggat cagcccgagg gcgctgtggg cggcggccag acgggcgaca ggaacgcgga
      421 agggcgagca gctgacgtac cggaggccaa gggaatggca gaaagcgatg ctgttcggct
      481 ctcccccatg ctccccgcag atgccgatct ggatggcggg ccgaacgggc cggcttttcg
      541 tgacggcaat cgacatcagt tggcctaccc catcccgatc gagggtgtgg aaagggtttg
      601 ccgggaggat gccctgccgg acgtattcgg caaggaattt tccttcagcg tcgtcgcggg
      661 aatatccgaa cgtcgtctgg ctcaggtcgt tggttccgaa actgaaaaat tccgcggatt
      721 cggcaagctg gtctgcaacg agggccgccc tcggcacttc gatcatggta ccgacaaggt
      781 agtgaacccg gcatccaaag cgagccatct gttcctcggc gatccgctcg gtcatttccc
      841 tcagccggtt catctcctcc cgggttccca caagcggcat catgatgtcc gggatcacct
      901 tcaccccttt ttgggagagg gcgcaggctg cttcgaaaat ggcccgaagc tgcatctcgt
      961 aaatctctgg gtagacgata ccgagacggc agccgcgaaa tcccagcatc gggttgtttt
     1021 cgtgaagcgc ggctgccttg gctagcttca ggcgaacggc atccgcctcc ggccccgatt
     1081 ctttcccctg gctagccaag aggtccagtt ccttctttag ctcgtgttcc ttgggcagga
     1141 actcgtgaag cggcgggtcc agaagccgga tggtgacggg caggcctgcc atcgcctcga
     1201 aaatcccctc gaagtcctcc cgctgcattc gctggagctt ttcgagaggc acgaggcgtt
     1261 cttcccgggt cgatgcgatg atcatttcct gcatgacggg aagacggtcg gcggccatga
     1321 acatgtgttc ggtccggcaa aggccgatcc ctttcgctcc gaagcctcga gcgcgggcgg
     1381 cgtcctcggg ggtgtcggcg ttcgcccaaa cctccagggc ggcgaattcg tcggcccact
     1441 ccagcaggct ccggatgtct tccgtgaagg agggctgcac cagctcaacc tttcctggga
     1501 tgaccagccc cctggacccg tcaatcgtga tccagtcccc tttccggaac ggacgcccgt
     1561 cgaccgtgaa aacctgggan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     1681 ggctgacacg ggaaacggcg gtttgccggt cgatgagccc ttcgttggcc atatccacgg
     1741 caacctggac ggcagccttc gcggtcctct tgccgtttcg cgtctggagc atgtaaagct
     1801 tgccccgctc gacggtgaac tcgatgtcct gcatgtcctt gtagtgtctt tccagaattt
     1861 ccgctgtttc aaggagttcg cggtagaggg cgggcatctt ctcccccatt ttctggatcg
     1921 gttccggggt ccgaattcca gccacgacgt cctccccctg ggcgttgatc agaaactctc
     1981 cgaacaatcc cttctctccg gtggagggat tccgcgtgaa gcagacgccc gttccgcaat
     2041 cgtcaccgag gttgccgaac accatggcga cgatgttgac tgccgttccc aggttgtcgg
     2101 ggatgttgtt gatcttccgg taggtgaccg cccggggcgt gttccagctg cggaaaaccg
     2161 cttcagtggc agctttgagc tggatccagg ggtcgctggg aaaatccttt ccggtggttt
     2221 tccggacgat tttcttgaac tgggaaacga cttcttccat tgcgtcggcg gggatttcgt
     2281 tgtcgtactg aaccttgagt tttcgccggg tcagcgtgag gacattctca aactcctcgg
     2341 ccgggacccc cagcaccaca tccgagaaca tctggataaa gcgccggtag ctgtccagcg
     2401 caaaccggcg gtcccctgag gtgtcactca gggcggagca ggtttcgtcg ttcagcccca
     2461 ggttgaggat cgtatccatc attcccggca tggaaaccgg ggccccggaa cggacggaaa
     2521 cgagaagggg ggtgccggga cctccgaagt tcttcccggt tttttcctcg agggatttca
     2581 tggctttttt cacttccggc cagatctcct ccatcgtgcc ggatgggtct tccatgtaaa
     2641 gcaggcaggc ttcggtcgtg agggtaaagc cgggcggaac gggaagcccg atgcgcatca
     2701 tctgggcgag gttggccccc ttgcctccca aaagggcctt catttccgcg ctgccttcgg
     2761 aaaaatcgta aaggagtttt ttccctttcc ccgtcatagt caagcctcct tttcgcacaa
     2821 agggtctcac gcccggatct ggtccaggat ttcctgggcg gtttcctcga tagcccgatt
     2881 ggtgacgtcg taacagcgac agccgatctt tctcatgatg ttccgggcga atccgagttc
     2941 ttcctcgacg cgctcccact gcgcgtaggt cgagacgtcg gcgtccaggc ccaggacccg
     3001 taggcgctcc ctccggatct ggacaaggtg ctcgggttta accatgagcc cgatgacctt
     3061 atccggcgaa accctgaaaa gctcgtccgg cggggtcgct tcgggaacga gggggatgtt
     3121 ggccaccata acccccttgt gggcaaggta catggaaagc ggggtcttgc tggtccgtga
     3181 gacgccgatg atcacaaggt ccgcctcggg cagaagatca ggactgcgcc cgtcgtcgca
     3241 gcggatcgag aactcgatgg ctttaaccct ccggaagtat tcctcgtcca tccgcctgag
     3301 caggccggga gtttccagcg gcgggcgtcc aaggcggttc tcaagggcac ccaggatggg
     3361 acccaggatg tcgatcacct cgactttccg ttcggccgcc cgttcaacga agtatttccg
     3421 cagcttgtgg tccaccatcg tgcagatgat gagggcgttc ttttcctgcg ctacctgaag
     3481 gacgtcctct gctttctctt cggtgctgat gtagcggaac ctcgaaagcc ttgccgcatc
     3541 agctccgaac tggctgtacg cggctcgggc gacatgttcc gctgtttctc cggtgaagtc
     3601 tgaaaccaca aaaacgtcaa ccgtcattcg cagaggtcat ctcccttcga cagcgcgacc
     3661 ggtttttatt ctttcaggag gccgagatcc ccgaccgaca taaacaggtc ttggcatcgc
     3721 gaaagcagag caagacggtt ctgacgcacg gcagggtttt cgtccatgac caggaccttg
     3781 tcgaaaaacc tcgtgatgga gggttcaagt ttcgccagca gggtcgtaac ctcgttccag
     3841 gcctgccgac tgagggcctc cctcacttgg ggttcaaggt tgctgatggc ctggtaaagg
     3901 aaagactcct cgtcctcgat gaaggctcct tcccggactt cagcgtcgaa ctctcctgcc
     3961 ttggagagga tgttcttcac cctgaccgct gccgttacca ggcccctgaa ccattcctgc
     4021 ccaaggaccg cagcgtatcc attcaggatc ttctggacct ggcaggggcg gttccacatc
     4081 aattgtaccc cgagggagac gagaccgtgg gaaaagccct tttcgcgaag ctggatctgg
     4141 aggcgctgga tgaagaagtc ccgcacagcc tccagggttt cacccggggc gtccagcgcc
     4201 tcggcaaggt cttcgaagac ggggaagaga tcgatgtcca ggtcgagatt ccagaggatc
     4261 tcgttgatgt tccgcgcggc ccgacgcagg gcataggggt cctgggatcc cgtgggaacg
     4321 agcccgacct tgaagcaccc gacgagactg tcggcacggt cgaccacccc gagaacggct
     4381 cctgcgggag aggaaggaag ggcgtccccc gaaaaacggg gcagcacctg ctcgtagatc
     4441 gctagggcga catccgggtc ctctccgttt tggagggcgt attcgcgtcc catcacaccc
     4501 ttgagctccg ggaattcgta aaccatgctg gtcaccaggt ctgccttggc caagatcccc
     4561 gcacggtcga tgacggcccg atggggttca agtccgggga cacgctccgc gagcttcaaa
     4621 gaaacggacc tgacgcgcat ggccttttcg tagaccgaac caagacgttc ctggtagacg
     4681 atcgctttca actcctcaac cctctccgaa agagggacct tccggtcctc cctccagaaa
     4741 aagcccgcgt cggacaagcg ggcccgaagc acccgctcgt ttccctcccg aacgacgttc
     4801 atgctggtgg cctgattgtt gctgactccg atgaagtgag gcaacaagtt cccgttccgg
     4861 tcacggacgg ggaaatattt ctggtggtgc ttcatcgagg tcgtcagaac ctcctcggga
     4921 atctcgagga aatccttgtc gaaactcccg gaaaaaggaa ccgggtattc cactaggaaa
     4981 aggttttctt cgacgagatc cgggtccagt tcggccttcc cgccgatttg tttttcaatc
     5041 gcagaaatcg cgctgagcat cttttcccgc cttttcgcgg gatccacgat cacgaaattg
     5101 gcgaacagca ggtccatgta ctcctttgga gaggggatct cgatgcttcg gtcccccatg
     5161 aagcggtgtc cccgtgtgat cctcccgctt tccaggctgc cgaaacggaa gggcagaacg
     5221 gcatttcccc agagggcgac gatccagcga atcggtctcg cgtagcgaat ggaagggtcg
     5281 tcccagtaca tgttcttcgg gaaaaccagg cgccggatca cccctgtaca gatgtccgga
     5341 aggagggcca cggtttccct gccctcgtgg cgcacttcgg cgagaacata ttcctgtccg
     5401 ttgacgatcg tggatttcag ttcatcgatc ggtacattct tgctgcgggc gaagccaagg
     5461 gccgctttcg tgggattccc ggcagcgtca aaggcctgcg tccagagggg gccgcggtat
     5521 tgctcaacgc ggtcctcctg tttggaggcc aaacccttga cctggatggc aagccggcgc
     5581 ggtgtcccca gagtggtgat tccctcgtgg tcgatatggg cttccgccaa ggattccgcg
     5641 aaaaggcggc gactttcctc cagcgcccag ggcatgaatc tcgcgggaat ttcttccgtg
     5701 ccgatttcca gcaaaaaatc ccttttctcg gtcatttttt aacacttcct ctagttggag
     5761 aatttcttca ggaggggata tcccatctct tcgcgctgct tggcaaacgc ctggcagcac
     5821 cggcttgcaa ttgccctgac cctgccgatg tatcccgtgc gctccgtgac gctgatcgcg
     5881 ttccgtgcat ccagaagatt gaaggtgtgg gagcatttca ggacgtaatc gtaggcgggc
     5941 aggacaaagc ccttgtcgag gattcgggtg gcttccgctt cgtaggcgtt gaagagcatg
     6001 aagagcattc ccgtgtcggc cagatcgaag ttgtacgttg agaattcgac ttctccccga
     6061 tgatggatgt ccccgtagcg aacctttccg acccagttca ggtcgtagac gttgtcgatc
     6121 ttctggacga acatggcgat ccgctccagc ccgtaggtga tttcagccgg caccgggttc
     6181 atgtccacgc cgcccacttg ctggaagtag gtgaactggg tgatttccat cccgtcgagc
     6241 cagacctccc agccaagacc ccaggccccg atcgtcgggg attcccagtc gtcctcgaca
     6301 aagcggatgt catggtccgc cggatcgatc ccgagcgccc ggaggctgtc gaggtagatc
     6361 tcctggatgt tttcaggagc cggtttaatg attacctggt actggtaata atgctgcagg
     6421 cggttggggt tttccccgta gcggccgtcc gttggacgtc tggagggttc cacgtatgcg
     6481 accttccagg gctcgggacc cagagacctg agggttgttg ccggattcat cgttcccgcg
     6541 cccacctcga tgtcgtaggg ttgctgaatg acgcagccct gttccgccca gaagcgctcc
     6601 aggcgcatga tcacttcctg aaaattcaat tgtcccacct cctgaaatct aaagactgct
     6661 ttcgatcaac cgggtcaaaa gggttgaaac tttaccgaag agtggaattt cgtcccggct
     6721 gaaggattct ttgcggaaat ctttgcctga gagcataaca gcctttttca ggcggttcaa
     6781 ttccttttct cccagagaga gacattcttt cgccggaaac ggtcctgcgc aggcggtaca
     6841 gagcagtccc tggagggtca aaaggccctg cggtgcaggt ttcccgcaag ccgagcaacg
     6901 gtcaaggctg gggacgatcc cgagcgccct gccccagcgg tagaggaaac gaaggcgtac
     6961 agccgccggt tcggttccgg cctccagggc cttgagcgtc cagaagagaa tcggaagaag
     7021 ttctgccgga gggtgtcccg gaagactgaa tctagccgca tccgcgcaaa tttccaacgc
     7081 cgcccgaagc ttttccttgt tctgtttcag agaccagaaa tcctcccgga cagccacttc
     7141 cctgacgtag atccttctcg gagagcggta cagcgtgaag tacccccaaa ccaagggctc
     7201 ggttacaccc gaaaggcggc ttctccctga agcggcccct gcggccgttg cccagaccag
     7261 gccggcttct ttcagcaaca ggagcaggtt cttggcacgc tctccggaaa ttgtggccct
     7321 gagcacgacc ccaagctgct ggtagtggcc ctgtggaagg ctaggcagaa aatccaacgt
     7381 ttcaccgccc gtagccgaac cgcctgagtt gctgggggga tttccgccaa tcgggcaaaa
     7441 ctttgaccca aagctcgaga aacacggggc agcccaggcg gttttccaga gatagccgcg
     7501 caaggcggcc gatttccttg agccttttcc cgcctgcccc aatcaggatc cccttctggg
     7561 aaggccgctc gacgtagatg gtcgcctgga tcaatgcctt ctgccgttcg ggatattcga
     7621 agggggtctt gaaagtttcg atctcgacgg caaccccgtg agggacttcg tcgcgtgtct
     7681 ggcgaaagac ctgctcccgg atcagttcct gtgcgaggaa ccgttccggg tggtccatga
     7741 ggatgtcttc cggaaacagg cttggtcctt cgggaagcaa ggaaatgatc ttttcccgta
     7801 gggcttcgat gttgaacccg gtcttcgcgg aaacggcgat catcgttacg ggaatgaggg
     7861 cgtcgagata gggtttcagg acggcctgca gggggatttc accgtgaagc cggtctgcct
     7921 tgttcgcgac gaggattttg gggaccgcca gggaaccgat gagctccagc aagccctgtt
     7981 cttcgggttc cggtgaatag gggccggcct caacaacgta aagaatcagg tcgacggatt
     8041 caagagaggc tttggcctcc tccaggagga attctccaag ccggtttttg gccgggtaga
     8101 cacccggggt gtcaacgaaa acaatctggg ccctttccga ggagtcaatg gcccggatcc
     8161 ggtttctagt cgtttgaggc cggtcggata cgatgcacac cttttccctg accaaggcgt
     8221 tgaccaggga agatttgccg acgttaggcc gtccgacgag ggtcaccagt cccgcccggt
     8281 gcccttcagg attcatggaa gggcctccca aggtcgaagc gttggggtaa aagttctgcc
     8341 aggccgtaga caacggggcg ctccccttgc tccaggatga tcgccatctc cccgttgaac
     8401 tctgccagaa cctgcaggca tgcgccgcaa ggaaagcagg gggtgccctc cttcccggca
     8461 accgctaccg cgatgggttt tcctgccccg gccgataggg cggaaaaaag tgcgatcctt
     8521 tccgcgcaga cggtgaggcc aaaactggcg ttttcgacgt tgcagccgat atggacgctg
     8581 ccgtcgtggg tcagcacagc tgcccctact cggaatcctg aatatggggc gtacgcgcgg
     8641 caccggccct gtcttgcggc ttcgagcaag gtttctgcat ccactggaaa agaaggccat
     8701 gtccgggggc tcattctccg acggccccct ctctccgctc tcccgtctgt tgccggtcta
     8761 accggagcag cttgatccgg tggtcttcga cttccaggac ctcaatgttc cagggcccga
     8821 actccagaac ctgtccctcg tccgggaaac ggccggcaag ggccaggatg aatcctgcaa
     8881 cgctgaaaac gtcctcggat tcgaaagggt attcgagagt ttcgctcaga tcctcaagat
     8941 tgacatgacc cttgaccagg taagtccctt ctctctcctt cacgatgtcg ggattctctt
     9001 catcgtattc gtcctggatc tctccgacga tctcctcgag aaggtcctcc agggtcagga
     9061 ttcccgccgt tcccccatat tcatccacga cgaccgccat gtggaccctc ttgcctttca
     9121 tgattccgaa aagttgggcg attttcatgg tttccggaac gaacatcgct tccctcttga
     9181 gcttcgagac cggcatgtca acctgtccgg aaacgagcgc cgggatgagg tccttgacgt
     9241 aaaggattcc cacgacatga tccaggctcc ctttgtggat cggcagccgc gaatgtccat
     9301 gttcctggaa aaccttgacc gcttcctgga tcgtgaggtt gtaaggaatg gaaacaacgt
     9361 ccattcgcgg caccatgatc tcgtgggccc gggtttcctc gaaagcaatc accccgtgga
     9421 tcatcttgcg ctcttcctct tcgagggcgc ccgtatctcc gccgatcgtg accatttgtt
     9481 cgatttcctc gcgggtaaca aaaggatgct gggaagagag gtccaccccg aaaagtttcc
     9541 caatgctccg gacaatgaag aggatcaccg cattgatggg aaacatcaga cggctgatga
     9601 aacggagcgg tgcgatcacc ttcaggagaa ggctttctgg acgggcgacg gcgaccgttt
     9661 tggggaggat ttcgctgaaa acgacgatca gcgaggtcat aaccaggacg gaaagccaaa
     9721 gtccctgact ccccacgagc tgaattaaga gcgcggtcgc aaccgctgtt gccgcgatat
     9781 tgaccaggtt gttcaacaca agggtcacag tcaggacgga ttggacgttc gacatcagcc
     9841 accccagtgt tttccggctt ttggggtgct tttcctggag ggcaagcaac ttgccctttc
     9901 ccgaagaagt aatggcggtt tcgctgaaac tgcagagcgc cgagagaaaa aggagcagca
     9961 ggagaagcag gagatggttg attaaactcg gtatgtccac ttccaactga cgatcagtcc
    10021 tccttgtaaa atcgcaaggc gattatacct tttgaaggta gcgccttaag agattttcct
    10081 gttcagagaa catcgccgat tccttctcct cggtgtcgtg atcccatgcg agaaggtgca
    10141 ggaatccgtg catcagcatc aaaaggaaat cgtacgagac atcacggctt tccgcatctg
    10201 aggataattt tacgacttcc gggcaaatca cgatatcgcc gagaggaatc tcgctccatc
    10261 cccgcggtgg agagaattcc cctccctgct cccacatggg gaacgacagg acgtctgtgg
    10321 gttcgtccag gaacctgtac cggcgattga ggtccttgat ggtctctgcc gagcagatct
    10381 taagagaaac acagaccatg ccagacaagg cgagatccgg ggccttttct tccaaataac
    10441 tctgccaggt ttcttccagg gcgtcgtaat cgggggaaga aatgccgaat ccctcaagaa
    10501 ggttttcctc gtcgtcaatc tgaaccgcta atttcacctt gttcacttcc tccctgttca
    10561 ggttcgaaaa gggacctggt tcccgtgttg tcccgtttat ggtagatgga acggagcgtc
    10621 tgaatgaagc tctccttgat caaggtcagg tccttcagcg taaagtccac aagttccagt
    10681 tgcccatcca gcattttcga gtccagaact ccttcgacgg tttctctgag ttcaggcagg
    10741 ccgcggatgc tccccccggc tgccctgacg gctgcctcta cggagtccgc cagcatgacg
    10801 agggcggttt ccttggaaga gggcttgggg cccgggtagc taaactgctc acggggcaaa
    10861 tcctcaccgc gggctttcga cttccgatag aagtagttca gacaggttgt tccatgatgt
    10921 tctgcaataa aatgccggat gcgggtcggc aggttgtagc gtcgggcaag gtccagcccg
    10981 tccttcacgt gggagagaat gacaagggcg gacatcgaag gcttcagctc gtcgtgcaga
    11041 ttttcaccgg gcatctgatt ttccgcgaag aattgagggc gcttcagctt cccgatatcg
    11101 tgaaaatagg cgccagcctt gaccagaagg ctgtcgagcc ccagcacgtc agctgccgcc
    11161 tcgcagaggg tccctaccat caaggtgtga tggtaggtcc ccggggcttc catctggagt
    11221 ctcttcagca gcgggttcga gggatggctc aggtccatca gccgaagcgg ggagagaatg
    11281 tcgaacatcg tgtcgagcat cggcagcagg gccacggtga aagttgccca gcccagaacc
    11341 gaaagcaggt atacgccctg gacatggctt tcaagcggca gaccgagcca ccatcggact
    11401 gccgtggcca caactgcggc cgaaatcccg gtcaacacct gttgtttcca aaggtccagc
    11461 cgggtattcg aacctcgaaa gaccaggtag ccggtaattg acgcggcaat gccggaaagg
    11521 gcccaaagaa tcagctgggc catcgatccc gagtaggaaa gcaaagcccc gataacggcc
    11581 cctccgagag agacgtggaa cgccagcgag gtcggtaccg tgagatagag ccatccggtc
    11641 agaggcagaa aagctagcgt gtcggccttg aaaatggctg acatgacctg aatcgtccag
    11701 ccggcactca aaaggcagat gataaagaca aaatcgcgtc gtgaaacctg gatttcccgc
    11761 cggtttgcgt agacggtcgg ccagaggctc cagacaaaaa cagaaaacag gatgaagacg
    11821 agcgagcggt agggaagatc ccccttgggg tacccttgag cctcgagcaa acggaacttt
    11881 tggggggtga ccatttcccc tcgggaaacg atcacgtcgc ccgggtaaat catgcggacc
    11941 acaggttcga tttttccggc agtttcccgt ttcagcttct cggtggcttc ctcgtccact
    12001 tggacgggtt cctggaaaat ctccacgagg atctgaaaga tggtgttctg gtcaggcctc
    12061 gaaagcggca ggcggatgat ccggtcccaa agcttcggct ggagatcctt gtcccaatcc
    12121 cgtacctcaa ggagggagag tccgagatcc ttggccaggt ggagcattcg gatccgtttc
    12181 tccgcaggca aggtggaaag cagatccctg agcgagtcgg acaacccttc ggtcgcgtcc
    12241 caacgtgaaa gctgttcaag ggaatcccgg atccgtctct cggcatgggt gtcccttgtc
    12301 atgacggaaa cgatcttttg ttccgcaatc tgccgaagac gttccgtgtt ttcttcgtca
    12361 atgtagcggg tgctcaggac cgcgagatag gttcgggggg aaggttcccc cagccggtat
    12421 ccctcgcggc tctcccccag ccaccagccg agtaggacga ggaaaaaaac cacgacgaga
    12481 agagccgccc tcagtgtcca gtaactgagg tcgatctccc tgagccggtg aagggcccaa
    12541 ggcttaggcc tgcctgccct gctcgtagga ttcataggcc ctcacgatcc tctggacgag
    12601 ttcgtggcga accacgtctc catgcccgaa acggatgaaa gcgattcccg gtatgccccc
    12661 gaggacctgc tcgacgattt ttagcccgga ctccttgccg gacggcaggt caacctgagt
    12721 gatgtctccc gtcacgatga cctgagatcc gaagcccatt cgggtgagga acatcttcat
    12781 ctgttcgggg gttgtgtttt gggcctcgtc caggataatg cagctctcgt tgagcgttct
    12841 tcctctcatg taggcaagcg gggcgatctc aatgacgctc ttgtcgatgt agcggaggaa
    12901 ccggtccggc tggagaaggt cgaaaaaggc atcgtacaag ggacgcagat aaggctccac
    12961 tttttccatc aaatccccgg ggagaaagcc cagcttttcc cccgcttcga cggctggccg
    13021 caccagaatg atccggttga agcgaccggc cttcaaggcg gcaaccgcat gggctaccgc
    13081 caggtaggtc ttccctgtcc ccgcaggtcc gatcgcgaag accagttcgt ttttcttcat
    13141 ggcctccaga tagattctct ggccttccgt ccttgcccga acgggtttgc cccgggtggt
    13201 caggcaaatg acatcctcgt acagtgaggc aaggttgaag tccgcccttt cccctacggc
    13261 ttccgcggca taccggattt ccgaggaacc gacggggtgc ccccgtcggc agacctccaa
    13321 aagctggatg agcagctttt ccgccttttc aacgcgctcg ggctcctggc cgctgacgga
    13381 cacacttagc ccgcgggcgg aaacacggac atccagcagg cgttcgatga gcttgagatt
    13441 ctcgtcgccg gggccgagca gcctggagag ggtttcctcg ctatccgccg aaatatgaac
    13501 ctgtctcgcc ttgtcgatga aggatccctc ccccgggctc cccggcagga aagcgccggt
    13561 tagttctccc ggatgacgga tttgaccgcc gggacgatgg actttatgac cgattccacc
    13621 tgcacccgca gtgtctcctg cgcgaaggga caggtgccgc aggcaccgac cagccggacc
    13681 gcaagcttgc cttcgggttc atcgaagcgg atgaactcga tatctccccc gtgggactgg
    13741 agtgcgggtt taatttcttc ctcgatcact ttcaggatct gttctgtggc ggtcatgtta
    13801 atttcctcct gtttttggta ggtttcttag gcgttcggtc tttcccctgt tttgggctat
    13861 acagggtaga ccccctcttc gacgggtttt tccaggccga cgtcccgcaa cgttgccctt
    13921 gcgaacttac ggacgagaaa atccttggcc acggccggaa acagaacgta gcttaacaca
    13981 tcctcgggct ggagaatcca tggagcgatc gccttcttcg cctcctcgag ctccggagga
    14041 agaacttctc cgggccgaca ggtaatggga gtttcctccc cgatgacctg tcggcggact
    14101 tccgggtcca cttctgccgg ggggcgccca taatagccca gcaggtactg ccggatttcc
    14161 ttgggaacga ccttccatct ccccatgaga acgtttagcg tcgcctgtgt tcccacaagc
    14221 tggctcgtgg gtgttaccag gggaggatac cccatttcct gccgaaccct agggatttcc
    14281 ttcaacacct cgtccagccg gtgaagggcc ttctggtcct tgagctggct caccaggttg
    14341 gagtacatcc cgccgggaat ctggtaaatc aggacgttga tgtcgacccc ctcgatcccc
    14401 atcacgaggc tgctgtattt ttccttgacc tccttgaagt gaagggcgac gggcaggagg
    14461 ctttccagcg tcatgccggt atcatagggc ccgcctcgaa gggccgcgat catggtttcc
    14521 gtcgccggtt ggctcgttcc catggcaagg ggcgaaatcg cacaatcgat gacgtctgcc
    14581 cctgcctcga tggcggcgag gtaggccatc gcggccattc cgctggtgta atgactgtgc
    14641 acctggaccg gaaggccggt ccgctccttg atcgccttga cgagggaaaa ggcgtcgacg
    14701 gggctcatga ggcccgccat atccttgatg cagatcgagt cggcccccat atcctgcatc
    14761 tggtgggcga tgtccgcgaa gttttccagc gtatggacag gggaaattgt gtaggaaagc
    14821 gccatctgca ggtgtgcccc ctcgcgcttg acctggtcgg cggcgatttc catgttgcgc
    14881 aggtcgttca gggcgtcgaa aaccctgata atgtccagtc cgttgccgat cgcccgcttg
    14941 acgaactccc gaaccgcgtc gtccccgtaa tgccggtaac caacgaggtt ctgccctctc
    15001 aggagcatct ggagcttcgt tttcttgaat cgctttcgca attcccggag ccgatcccag
    15061 ggatcttcgt cgagaaagcg catgcaggta tcgaaggtgg ctcctcccca gatttccatc
    15121 gccccgtagc cgacttcgtc cattagctcg gcgaccggga tcatgtcctc ggtgcgaaga
    15181 cgggtggcca tgatggactg gtgagcatca cggaaggccg tttccatgat cttgactttt
    15241 ttccccggaa cctgctcccg gttttcggga cagggctcag tgcttccgtt cggacgatca
    15301 ggcttgttct ccatgacggc ttccctcctc atccatcctg tccgttcaac tcaacaggat
    15361 tgcttagctt tttcccaaaa atcgtcgagt tcgtcaaggg tgaattcttt ccaaggtctg
    15421 ccgctttcgg ctacagaacg ctctacgaga cggaatcggt ccgcgaattt ttgggaggct
    15481 ttctgcaggg cttcttccgg attgaccccg caatggcggg acaggttgac cgccgcgaag
    15541 aggagatcgc cgatttcctc accgattcgg tttccttttt cagaggaaag ttcttcctgg
    15601 atctcggcca gttcctcccg gacctttgcg atgacgggag tagcgtcgcc cttcggccag
    15661 tcgaaaccga acttggccgc ccgctcctgg attcggaagg ctttcagcag agaaggcatt
    15721 gacctgggga ttcccgaaag gattgaagag tcggtgccgt tctttttctt ctccacggtc
    15781 ttgatggctt cccagttgcg catgacctcc tctgaatccg ccacgttcag gtctccgaag
    15841 acatggggat gtcgatggac cagcttcgag cagattgcct gcaggacgtc tcggatcgag
    15901 aaggatcccg tttccgatgc gatctgggcg atgaagacga cttgcaggag aaggtcgccg
    15961 ctttcttcca ggatcgccct atcgtcttcg ttctctatgg cctccacgag ttcataggct
    16021 tcctcgatga tataacgtct cagggtttta aaatcctgct cacggtccca gggacagcct
    16081 tccggtcccc gaagtttttt catgatggaa accagccggg aaaagtcgcc gttcggagcg
    16141 tcatggcggt gttccattct tttcagtcct ccgttcattt atcaatgaga aaattgctgc
    16201 caatcgatca atttcacgag agaagcgatt ccctggaaac ctcccgggcc gaccgcccaa
    16261 tttttcccct ttttccatcc ggtagcccgc tgaagggcgg gaattcctgc caatgtcccc
    16321 ttaagggttg tttctttcga gctgacctga accgagtcaa tcccgagacg gtctccttca
    16381 atccggagca gggccagtcc gaagatccct tcgacggcgg gcggaagagg gccgaacctg
    16441 tctcttactt cggctttcag gctgaaaagc tcgttcctat cttgctctcg caggaggcgc
    16501 ctgtaaaggg cgattcggac acctgtctgt ggaatgtacg aaggggggat ctgcaacgga
    16561 gccaggattt cgattcgtgt cccggcaagg ggctttcccc tcaaaatcga aacctggtct
    16621 tccagcatgc ggcagtagag gtggtatccg acccgttcga catgcccatg ctgggctgtc
    16681 ccgacgacct ggcctccgcc gcggatttcc aggtcccgct gggcgagttc gtaccccgac
    16741 ccagggtcgg ccaactcgcc gatcgcttcg aggcgttccc gggcttccct ggaaagggga
    16801 aactccctgg ggtagaaaaa gaaagcaaaa gcctgttcct cacgccgccc taccctccct
    16861 cgcagctggt aaagctgcgc caagccgagt tcatgggcgt cgtccacgac cagggtgttc
    16921 gccctgggga tatccagccc gctttcgatg atggtcgtgc agacgaggac gtccgtttct
    16981 cccgaataga atcggagcat ggcctcttcg agagccccct ctcccatctg cccgtgcgcg
    17041 atgtcgatcc tgagcgaggg gaaaagggtc ctgagggttc ctgcacgggc ttcgatgtcc
    17101 gcgatccggt tgtgcacgaa gaaggtctgt cctccccgtg ccttttccct caggaccccc
    17161 cgaaggagca gatcctccct ccaggggctc acgacggtca ggaccgggtg tcgttggtga
    17221 gggggagtcg agatgacgga gatgtccctt agacctccaa gggaaaggaa cagagtccgg
    17281 ggaatcggcg tcgcactcat catgagaacg tcgacatctg gcttcagttt tttggaatgc
    17341 tccttgtgga ggaccccgaa ccggtgctcc tcgtcaacga taaccagccc aaggtccttg
    17401 aaccgcacgt ccttctgaac aaggcggtgg gtcccgatca ggatgtccac cttgccttcg
    17461 gcgaccgagc ggacgatctc ctgttgcttt ctcttcggga cgaagcggct gagggcttcc
    17521 acgcggaggg gaagttctcc gatcctggag agaaaggttt cataatgctg ctgtgccagt
    17581 agcgttgtag ggaccagtac cgcgacctgc ttccccgatt ccgcggcttt gacggccgcc
    17641 cggaaggcca cttcggtctt tccaaaccca acatctccca ccaggagacg gtccatgggc
    17701 tggggtttct ccatgtcctt cttgatctcc tggattgccc gcagctggtc cgccgtctcg
    17761 gagaacggga aagacgcctc tgcctgggct gaaaaggggc cgtctttggg aaaggcgaag
    17821 ccgggagaga cttcccgaag ggcataggtc tgcagcagtt ggagggctgc ttcgtgaacc
    17881 ttgcgtcgga cacgagcaag gctttttttc caatgacctg atttgagaga atccgcggaa
    17941 ggctcggccc cacgcggcgc gaggtagggg gtgaccttcc agagctggaa gggaggaacc
    18001 aaaagccgct gtccaccggc gtactcgatg acgacgtatt cctgtttttc cgactggaca
    18061 ctcgggacgc tttcgatgcc gacgaaccgc ccgattccgt agtcctcgtg caccacccac
    18121 tcgccaatcg taagcctttc actccattcg aggggcggca gggagactcc ttccgtcggc
    18181 tgaagagccg tgattccgct gatgtccgag tcggaaacaa aaacgaggtt ttcggaggga
    18241 ctgatgaaac cggaacttag cggcgcttcc ttcaccgcga tgcccttttc ggtgagcctc
    18301 aacgccagag agcggtcggc ggtgtggacg gaaaccttca atcccaggtt ttgccattcc
    18361 gagagggcag cgtcaagggc ctgcaaacgg cctttaaagg aggggacggg tgccaggccg
    18421 attttcccgg aaacacgctc ggtcgatgcc ttgatccgga gaagcggaaa acgggagagc
    18481 cggaggtaga cggagtccca ttcgggtagt ccttccacac cggcttcctc ctttagccct
    18541 tcccagaccc aacggtaatg atctgcctgg gaagacgttt cggcaggttc aaagagaaag
    18601 atgcgggtgt tttccgggaa aaattcctcg agggaagggc ttcttccctc cctgaccgcg
    18661 gagatttcga cggtatcgac gggccctgtg cttcgctggg tctgcgggtt gaaaaaacgc
    18721 agcgactcga tgcgatcatc caggaactcc acacgaaggg gacttcggat cgaggggtcg
    18781 aaaaggtcca ggatgctacc ccgcacgacg aattgtccag gggaccagac ggtatcgact
    18841 cgttcatatc ccgaagagac cagccagccg aggagagatg cgcgggcgat tcgatccccg
    18901 agtctcaggg aaacctttcc ttcggccagt gcgcaaggcc ccatgagtgc acccggcgtg
    18961 cagaccagga tcccgagcct ggttttccat cgggcaagtt cttcgcccct ttggatgcgg
    19021 atcccgatat tcgaaagtcc ggaggttgtc agggggattt cagggaggca ggcgagggct
    19081 agattgtcct cggtcagtgt tttcaggtcc gaaaagaatt ccatttgcct gcgcgtgtcg
    19141 ggaaggatga cgagggccgg aaaggggaag tccctcaata gccaggccct ggcggcgctg
    19201 ccggcaatca gggcctgatc cctgttctgc cagggccccc aatcgccctt tcggatgaga
    19261 tccaaaggca aacccggaga atcagcctgc attgacgcgg ttcatggctc ggtcgatgcc
    19321 gagtcgaatc cagagcatta cggcgtccgc cgctttttcg aggaaactgt ccagttggcc
    19381 ctgctcttcg ctgtcaaagc ggtccgttac gtagtcggcg agtcctttcg cctgtacagg
    19441 ccgcccgatc ccgcacctca gccgcgggac atgaagggtt tgaagagccc cgaggatcga
    19501 ggcgattccg ttgtggcccc ccgcagagcc gctcgctcgc aggcggagcg taccgaaagg
    19561 cagggccaca tcatcgacga cgatcaggca ttcctccggc gggagattga gttccctgac
    19621 cgcggcaaga acggatttgc cgctgaggtt catgtaggtg agcggcttga gaacgaaaag
    19681 ggagtcgctt ccgacccggc agggacccca aagaaggccg tggaaccggt tcacggggcc
    19741 cttgagatgg aggcggctga tgacctgatc ggccaccagc cacccaagat tgtgccgtgt
    19801 ccaggcatac tcgagaccgg ggtttcccag tcccgccacc agtttcaatg gacttatgcc
    19861 tcctcctggt tcttggcttt gcctttcgct aggacttcaa cttccttctg ctcggattcc
    19921 tccgcaggag cggcttcggc agcgcgcggc tcagcggcga tgacgacggg caagtctccg
    19981 tcgtcgacca gctcggcgga ttccggcagc acaaggtccc gcaggcggat ttcctcgccg
    20041 agcccgagat ttttaacatc gacggaaagg acgtccggga tttcccgggg cagaacttcg
    20101 attgaaactt cgtggaaaag ctgttccagg agccctcccg ccttgacgcc ggggcaaatg
    20161 tcgcgattca gaatttcaat tggcaccttc acctggacct tctgtcccga gacaagctgg
    20221 taaaaatcga tgtgaaggag atttccattg aggaaatccc gctggacatc cctcatcagg
    20281 gccatttcag tcttaccgtc gggaagcgtg acctccagcc tgtaggtttc ccagtgcccg
    20341 gagttcagcg cgggaaggat atcccggttt ttgaccttga ccgggatcga atcggggtat
    20401 cccctcccgt agaagacggc ggggacatat ccttccgccc gaagctttga agcctcctgt
    20461 ttgccgcttt tttccctcgc ttcgagggta acctgcaaaa gttcggacat gatgtttcct
    20521 cctcccagaa aatgcagggc cggtcccgcc tgccccgcct agtaaaaaag tatgctgacc
    20581 gaatggtcgg aatggatgcg gttgatcgcc tccgcgaaaa ggggggcgat ggacagcacg
    20641 gtaatattat ccaacttttt ctcttcgggc aacggaatcg tgtcggtcaa cacgagctgg
    20701 tggatcggtg agtttctcag gttttcaatg gcttttcctg aaaggacccc gtgcgtcgcg
    20761 caggcgtaga ccctctctgc ccccctctcg agcaaagccc gcgccgcgtt gaccatggtg
    20821 ccggccgtgt cgacgatgtc atcgacaagg atcgcaacct tgcccctgac atctccgatg
    20881 atttccatca cttcgcagaa attggcgact tcatgtgacc tccgcttatc cacgatcgcc
    20941 aggtccgcgt taagctttac cgcaaaccgc cttgcccgga ccactccgcc gatatccggg
    21001 gaaatcacga cgattttttg ctggtcaaag gcttccttta gattctcctt gaaataggaa
    21061 gccaggagcg gaatcccggt caagtggtcg acgggccggt caaagaaccc ctggatctgc
    21121 cctgcgtgga gatccgcgac aacgagccgg tctgccccgg ctttctcgat gagattcgct
    21181 acaagcttcg acgtaatggg ttccctggct ttcgttttgc ggtcctgccg ggcatacccg
    21241 aagtagggcg tcaccacgtt gatccggtac gcagaggccc ggcggagtgc gtcgatcatg
    21301 atcaagagtt ccatcaggtt ttcgttcacg ggctcagagg tgggctgcac cacgtaaaca
    21361 tcggctccgc ggacgctttc ctcgatggag agcccgattt ctccatcgga aaagcgaaac
    21421 agcttcgaag aagctatggg aaccccaagc gtgtcggcca ccttctgggc gaaggccggg
    21481 tgagcggatc ccgtgaagat tttcatctgc ctcaactgcg tgaccatcct tcttcctcct
    21541 tccgcgaaac cgctttcttt ctgagtgccc agccctcaat gtttttctgc ctggcccggc
    21601 cgacggccag ggccccggcc ggaacatcct ctgtgaccgt tgacccggcc gcggtgaagc
    21661 agccatcgtt caggacgacg ggtgccacaa gcatcgtgtc gcttcccaca aaacagtcat
    21721 ctccaatacg ggtcggattc ttccgcgaac cgtcgtagtt gcaggtgatt gtgccagcac
    21781 cgatgtttgt gcgcttgccg accctggcgt cccccaggta ggaaaggtga gggactttac
    21841 tgttttctcc gatgaaggta tttttcactt ccacgaattt cccggcatag gcgccgcttg
    21901 cgagacagct gttgtcccga atgtaggcga acggtccgac tttggcaccg gggccgatct
    21961 cgctgtccgt cacgcaacag aaagcaacga gatgcgcctt ttcccggacg agcgcattgc
    22021 ggaggatcga gtggcttccg accactgcac cgcattcgat ctcggtcttt ccccagatct
    22081 ggacatcggc ctcgatccac acatcgggct ggaggaggac atccggtcca atccacgtgg
    22141 tttggggatc catcatcttg atcccgtttt tgaggtgctt ccgtaggatc cgcccgcgca
    22201 tttccctgtt ggcttctgcc agttgcaggg ggtcgttgat cccgaagaga tcggttgcct
    22261 tttcccacaa aaggggttca acccgcaggc cggactctga taaaagcgga aagacgtcgg
    22321 gcaggtagaa ctctccctgg gcgtttcggt ttcccagccg atcgatgacc cttaaaagag
    22381 ccacggtgct aaaaacatag atcccgctgt tgacttcgtt caccgagtgc tcgtccgggg
    22441 aagcgtcttt ctcctccacg atacggcagc ggtccccgtc gcggataacc cggccgtatc
    22501 cgaaggggtt ctcgatcctg aaggtcatta cggtggcagc gtttcc
//
LOCUS       DAOOVN010000016        20548 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_1759_length_20548_cov_14.989704, whole genome shotgun
            sequence.
ACCESSION   DAOOVN010000016 DAOOVN010000000
VERSION     DAOOVN010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 20548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20548
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1759_length_20548_cov_14.989704"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..805)
                     /locus_tag="PLY83_02965"
     CDS             complement(146..805)
                     /locus_tag="PLY83_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29031.1"
                     /translation="MNRIEFARRMGKLLFGLFLFALGIVVTMKANLGFGPWEVFHQGI
                     SLKVGLSIGNVSILAGLLICVLVFLAGEKLGLGTILNMVLIGFFMDRILALGIIPRMT
                     GFLPGLLMMFAGLFIISLASYFYMGSGFGAGPRDSLMVALERKTGLAVGLCRGIVEGS
                     AVLLGWLLGGPVGFGTVLSAFGIGFCIQVVFRSLGFDARRVQHETLEVTFRKLLPKNG
                     G"
     gene            complement(823..1131)
                     /locus_tag="PLY83_02970"
     CDS             complement(823..1131)
                     /locus_tag="PLY83_02970"
                     /inference="COORDINATES: protein motif:HMM:NF017273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HOV29032.1"
                     /translation="MNCSDMEGIAVICLSALLVWGIRFLGLVFGEKLPRTGRVKRGMD
                     ALPGAIFAALVFPGIAGAGIPGGVAAGVIFVATRKTGNVLLALLLGVGTVALLRGAGF
                     "
     gene            complement(1128..1805)
                     /locus_tag="PLY83_02975"
     CDS             complement(1128..1805)
                     /locus_tag="PLY83_02975"
                     /inference="COORDINATES: protein motif:HMM:NF015549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HOV29033.1"
                     /translation="MKGELARGAGAAIPVSLGIVAYALVFGILVAGKGIGIKTLAFMN
                     LAVFSGSAQFVLVGMWQTGVSAWQMALAAAVVNVRYFLLTAAVAPMMEPYGWFERAWR
                     VHFVTDENWALAMAERRKSPIGANYLLGGGILVMATWSLGTLAGHALGVSIRHPERFG
                     MDFAFTAIFTALAVTLWRGKHDLLPWAVSAGVALLAHRVLPGAWYVLLGGLAGAFAAA
                     AGKGDPS"
     gene            complement(1812..2507)
                     /locus_tag="PLY83_02980"
     CDS             complement(1812..2507)
                     /locus_tag="PLY83_02980"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HOV29034.1"
                     /translation="MKTIGLVGGVSWESSKEYYRIINEEVARRMGGLHSAECVMTSLD
                     FDPVAKAINAGDHETYRQLVLRSAARLKGSGVDFVLICSNTTNAFAEDVERLLGVPVI
                     HIADAMGAAVVKRGFRTVGLLGTRSLMEADTVKGRLERKWGLSVLIPDVEDRVTVNRV
                     IFDELCAGKILESSREEYLRIMGKLADRGAEGILLACTEIPLLIQQEHTDLPVFDSTE
                     LHALMAVDLALEG"
     gene            complement(2504..3412)
                     /locus_tag="PLY83_02985"
     CDS             complement(2504..3412)
                     /locus_tag="PLY83_02985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HOV29035.1"
                     /translation="METVRIKQVDAFTDVAFGGNPAGVVTSAEGLDEGQMQKIAREMN
                     LSETAFVTPSDKADFRVRFFTPNSEVDLCGHATIGTFAALHEEGRLDAARSVFTQETK
                     AGVLEVERSFRGDRPVFTMTQAKPRFRETGASREEVAGLLRVEASEFLDVEPLAVSTG
                     LWWFVVGFRRLTAVQALAPDLAEIRRFSERHGLIGIVPFCLETVDPGADLHMRAFAPF
                     VGIDEDPVCGTGNGSVGAFIGRNGLVGFTERLDLASEAGMEVNRPGRVFVSLEKRDGE
                     ISRVKVGGSAVTVLEGVLRFRREDCR"
     gene            complement(3440..4957)
                     /locus_tag="PLY83_02990"
     CDS             complement(3440..4957)
                     /locus_tag="PLY83_02990"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOV29036.1"
                     /translation="MIRLHLERDSGTPLFEQIAEAFRERILGGLLLEGTPLPPERDLA
                     KRLGVNRSTVVRAYGELKADGFVEARVGRGTVVAAGGTGLDRESGRAEPLEWDCLLRP
                     ETASCGDLFSEMMVVNGREGVISFAPGFPDPDLLPVEEFLALQRDLGISGFRSQFLPS
                     PVEGFAPLREKVAELLADRGIDVPSREVMILSGSQQGLDFAARALLSPGDTVLAEKST
                     FFGALEIFRAAGARVAGIPMDRDGMRTDLLEGAIRRHNPRLIYTLPTFQNPTGITLSL
                     ERRRELLETAGRFGVPVLEDDPYGELRYEGRPVPPLKALDRRGIVIYLSSFSKVLSLG
                     FRVGWVSAPAPVIERFSRLKQMTDLHVNTPAQILLDRFLGEGRYGPHLERVRKAYREK
                     RDRLAESLTRYRTRIGWDWDLPEGGYYLWCSLPGFFPLRAFMARAAENRVAFLPGTAF
                     YADGHPRQSRIRLNFTHVRLEEIEEGVRRLARAAEKARENGSGEFGPFEGQEPIV"
     gene            complement(5156..5602)
                     /locus_tag="PLY83_02995"
     CDS             complement(5156..5602)
                     /locus_tag="PLY83_02995"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOV29037.1"
                     /translation="MKRVVVAIDHSAVSRSLMDAAFDLAAREKDVTLYFFHAVEPLRT
                     GWRSRSKEIDEVLLAKVRKEVHDWVHAAAAEFGKPLQDLRVAVRPGFPAEAILDFADG
                     VAADLIMIGHRGNPERKRFVLGSVAAEVVAHATCSVYVHRPRVLGE"
     gene            5756..6394
                     /locus_tag="PLY83_03000"
     CDS             5756..6394
                     /locus_tag="PLY83_03000"
                     /inference="COORDINATES: protein motif:HMM:NF024628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HOV29038.1"
                     /translation="MCRMLLANRKGIEELKLALAAIPGCSTLEKYLRDLELRDGGQGN
                     GYALLKDGRLVRLEKGVNLAVGTIAKVMETEDYDWVLFHTREASTGSVSDANCHPFRV
                     SGKAELVAAVNGNEKSVMELARAAGGITDSEFILKLVAEMGLPLKETLAIFESNFFGF
                     YNGRPFVKKGNRDMLTWEKGEGLVFASDFPFGMEGLRKPEPDFFWMEGSTGA"
     gene            6564..7556
                     /locus_tag="PLY83_03005"
     CDS             6564..7556
                     /locus_tag="PLY83_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HOV29039.1"
                     /translation="MLFLSREQILSFFTMRDAIEADKKAFVLHTEGKAKVPLRINLET
                     EDKSGQCMFMPAYVGGELNMAGVKMVSFFPGNAQKGIPVVPATVALIDGTTGLVTAIV
                     EGTTLTQLRTAAISGAATELLSNPDSRVAALFGTGGQAPAQLEALMTVRSLREVRVFD
                     VDPGRIQNFVTKYRPLADRFGTALVGAKTPDEAVEGADVITTVTTSGKPVFDGSRVKP
                     GCHVNGVGSYTPVMRELPLELLNRAGRIFVDNREAVLAEAGDFLIPMGEGRFSPERIA
                     GELGDLILGRVPGRTSPEEITVMKTVGFATLDVVAAAAIVEKARGLGIGSEIAL"
     gene            7608..8270
                     /locus_tag="PLY83_03010"
     CDS             7608..8270
                     /locus_tag="PLY83_03010"
                     /inference="COORDINATES: protein motif:HMM:NF019947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOV29040.1"
                     /translation="MPRECHPLLKPLVPVVRLLGAMLGGDCEVVLHDVSGEVPFIVAI
                     ENGNLSGRDIDAPLTDLGRFLVTSPEAEPVDFLANYPSEGPGGRAMRSGVAMIRDGSR
                     RLVGLLCLNFDMTKGFFLKDLGTFLTTVRPLSFSAFQSEDFRGITGDPALNLLEKARQ
                     DFGKPLRYLDREERIACIRFLEEQGFFQLKGAVSLLTKETGKSRYTLYADLRAVRSGK
                     EE"
     gene            8276..8890
                     /locus_tag="PLY83_03015"
     CDS             8276..8890
                     /locus_tag="PLY83_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HOV29041.1"
                     /translation="MVKTAIPEEKIREAIAFHGHWCPGLATGIRVAELALREVGRAGD
                     EDIVAVAESDTCAVDAIQVLTGCTVGKGNLVLLGIGKMGFSFYRRRDGKAVRIVARPQ
                     KPGEGSDEYRRLQAKSNDGTLSGEEALRFETLRQERGRRILEADLDELFEIKKPLRPV
                     PPHAPMKRSLTCEACGESVMETKARLSDGRVLCPDCFEREVPRP"
     gene            complement(8986..10512)
                     /gene="yfcC"
                     /locus_tag="PLY83_03020"
     CDS             complement(8986..10512)
                     /gene="yfcC"
                     /locus_tag="PLY83_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HOV29042.1"
                     /translation="MPETNQAPVAPKATRVPDTYIIIFFVVLLAALLTYFIPVGSYQT
                     HEISYKMGEQTKTRTVLIPETFKLATDDQGNPVKKGMPVFAPNGDMGILNYVFEGFVS
                     GSKWGSAIGVMAFILVIGGAFGIILRTGAVESGLLKMISRARGMEAAIIPILFVLFSL
                     GGAVFGMGEEAIPFVMILCPLFVAMGYDSVTALFSSYVATQIGFATSWMNPFSVAIAQ
                     GISQIPVLSASGFRIAMWAGFTLLGIVFTWFYAKRVKAEPTRSISYKSDAFFREDLEK
                     NKAVESHFGTGHSLVLLTLAAGIVWVIWGVVEKGYYIPEIATQFFTMGIVSGLIGVAF
                     KLNGMTVNDIASSFRDGAKDLVGAAMVVGMAKGIVLILGGDSPTDATVMNTVLHAIGN
                     AIAGWTPAVSAWFMYLFQSVFNFFVVSGSGQAALTMPIMAPLADLAGVTRQVAVLAFQ
                     LGDGFTNLIVPTSGVLMACLGAARLDWTTWAKFQIKFQGLLFAVGSLVVVGAVLMGLN
                     "
     gene            10669..11400
                     /locus_tag="PLY83_03025"
     CDS             10669..11400
                     /locus_tag="PLY83_03025"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOV29043.1"
                     /translation="MTELNRPPTDRPFQAFEVVFWHVLDEMFEERLLPGDVLYETALS
                     QELGLSRTPVREALSRLVAEGLLENPVGTRGYRVPDLSVEDMQQVFQAREILESSAAA
                     LAAEHARKGDIGELEEIHRREILFSSQENRREYCRANVEFHFAVMRIGGNRYLERAFR
                     PVFWRSQLYVNYLAEFHPLNGEERTAESGHNSPAEHAALIRAIARRAPRAAAKAAADH
                     LRSTRAYRILLDTERARHIFGGSSR"
     gene            11430..12614
                     /gene="iadA"
                     /locus_tag="PLY83_03030"
     CDS             11430..12614
                     /gene="iadA"
                     /locus_tag="PLY83_03030"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HOV29044.1"
                     /translation="MFLLVENAEIYAPERLEAREVFVAGERFAWIGNGFPAKSTLPGI
                     ERIDGTGCLLLPGFIDNHVHIIGGGGEGSYRTRTPEISLTDLTTAGVTSVVGVLGTDA
                     VTRHTASLIAKARALEEEGISAWAMLGSYQLPVRTLTGNIQDDIVLVDKLIGVGEVAL
                     SDHRSSQPLQEEIARIAAAARVAGMLSGKGGIVNVHMGDGPRGLSMIKAVAQETELTL
                     AQFLPTHVNRNPELFAEAIEYAASGGHVDLTTSTTPVFLAEGEVKCSVGLKRLLDAGV
                     PASRVSFSSDGQGSMPDFDEKGHFRGLTVGRCSSLWGEVVDAVRDEGIPLETALQVIT
                     SSPADHYRLPGKGHVTAGADADFVLVDRETMAIRTVVARGQVMVRDGRPVVFGTFEKR
                     EQ"
     gene            13025..14164
                     /locus_tag="PLY83_03035"
     CDS             13025..14164
                     /locus_tag="PLY83_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29045.1"
                     /translation="MTEEKKSSILTFFMNYGWYRKYLLPGFIAQSVLIAGGYGTGREL
                     VEYFVQYGPTGGLMGMGLTTILWAALFALSFEFARAFKAYDYRTFFEKLIGPGWMVYE
                     AAFIVLLFLIMGVVGAASGSILRDSFGLPPLVGSGLFLVFVAYLTYSGSKAIEVALSW
                     WSYVLYAVYILFLYFGISRFGDAISANFAAHVVKPGWAMGGFKYAFYNIAVVSTVLFS
                     LNYLETRTEAILSGIMASLICILPAGLFYVVTVGFYPGVLNMEIPANGIIANLGVRFL
                     LPVWLIVLFGTMIETGVGFFHSINERINSTLVAKRGRGMSNLARGAVGAVLALMGLAI
                     SNFGLIGLIAKGYGTISWVFFILQGVALFTIGLYKLSRQSSGKTA"
     gene            complement(14239..15339)
                     /locus_tag="PLY83_03040"
     CDS             complement(14239..15339)
                     /locus_tag="PLY83_03040"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOV29046.1"
                     /translation="MWRGKLPREIFEIAQQGYAAAEPVVYPEGIEVIDCGLGTNPLGM
                     PESLRRFLEKGIPCDLCDYPEPEPEGLKQAIATVHDAWKVSPDQLLVGGGSIGLLVSL
                     FRILLRPGSVLSGVSPQFTDPVLQALYNGASYEPLRLVPPRFTIDTAFVLEALEKKPD
                     VLYLDRPHNPTGQVMSLEDVARLARFAMEAGTWVISDEAYGDFLPDDESAAVLDFPNL
                     VTCRSFSKGPGAAGIRAGFAVCRDPDLAALFRKVQPPFVIGTLDVRMALEILKDPAPL
                     QETRAYVRAAKARILDAVSKKPGWFVADTDERVPILLLSQASGNLVRRLAAVGVSCEG
                     GSGFFGLDDRSVRLRVPAPAKLEALLERLANA"
     gene            15485..16585
                     /locus_tag="PLY83_03045"
     CDS             15485..16585
                     /locus_tag="PLY83_03045"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOV29047.1"
                     /translation="MKKWLVSALAFALLAFFPVPSWTVEPPGYVVYQVVPTYFNTLEE
                     AMAAFSQIRSQISISGGAIQGLHLSREALSFKTAMGSPLSIPFASFTLLRLLEIPKAP
                     DPWKWALAAKVSEKGNPVFISAASRDAAEKMFSILWSFAAAAGAPLELRDVGAAVVDP
                     TPQDLKINGLKEPKGGVVLWVSVGGASERAGLQHGDLILSINGLPVDNNTEFQEKVWP
                     TIAPSRGVESYDLEILRKGAPMPLKIKLPSLETLPRPPATLSFTPPPAGPAAFQKQPP
                     KLGFSLRSLEPAENAALNGKSGAVIAELTPGGEAEKAGMQVGDILVACNGQPVPGPKE
                     LGGLLALGENTFTVLRKGLQLTFRLNPPVASY"
     gene            16770..17222
                     /locus_tag="PLY83_03050"
     CDS             16770..17222
                     /locus_tag="PLY83_03050"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOV29048.1"
                     /translation="MKKVVVAIDGSEESKGVIDYAVHYANREADAEMHFLHVIGTEER
                     GRVFVEGYAVELPTVGERVKQDFEEFVRAQIAKSGRTIPRMSVTVLPGIPYEQIVKFA
                     EENDADIIMIGHRGLSNLKRFFLGSVAAKVVAHAPCSVYVHRPKGRQA"
     gene            complement(17292..18545)
                     /locus_tag="PLY83_03055"
     CDS             complement(17292..18545)
                     /locus_tag="PLY83_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOV29049.1"
                     /translation="MKRNLFVLLLAGFCLCGTFSGIPAMATVPAEGCSTGPVVPEEAV
                     GKAVNRFAAGLLRLSAENQPGNLMISPVSVHLALTMAANGAEGSTKAAMLKGLAGDGL
                     TLDRLNRSIRAWTSGVSRKGGKTTLGIANSIWFDKGYVPSRFFLQRNRDFFGAEIRRI
                     DFRDRGAPGIVNAWVKTATRGTIDRILEEIRRDEVLFLVNAVYFKSDWQVPFEKSETR
                     KEVFHAPGGDIETDFMHRTGRMTVFQLKGATGVCLPYEDGRFAFFALLPEEKTAPRAW
                     LARQDPATLFEGIARRMAEKPALEVQLALPKFESRYEDTLEDELKKLGMGIAFDPARA
                     DFSAMSERRTKDLFIGTVRHKTFVRVDEKGTEASAVTSVGMRMTSLPSVDRKISFDRP
                     FLYGIVDLKAGIPLFVGILENPANP"
     gene            18740..19144
                     /locus_tag="PLY83_03060"
     CDS             18740..19144
                     /locus_tag="PLY83_03060"
                     /inference="COORDINATES: protein motif:HMM:NF025589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HOV29050.1"
                     /translation="MAKLQKIEGIGEAYAKKLEGAGITTTEGLLEKGATPKGREELAK
                     MTKIPASQILKWVNHVDLFRVKGIGAQYAELLEAAGVDTVPELAQRKPDHLLQKMIEV
                     NEKSKKVRQLPSINRVKDWVEQAKKLPRVVTY"
     gene            complement(19189..20253)
                     /locus_tag="PLY83_03065"
     CDS             complement(19189..20253)
                     /locus_tag="PLY83_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HOV29051.1"
                     /translation="MYPRLVMDAGKVRENAAEVVRRAAKLGISVFGVTKGFCADPVVA
                     RAMLEGGCSLLMDSRLKNLRRLREAGFTEPLMLLRIPMLSELKDLVQVADGTVVSMPE
                     TVLALEEACEAAGKSVDVMVMADVGDLREGLWPDEMEAMGKTLASCRRIRCMGVGTNI
                     GCFGGVLPTPENMNLLIECRNRLSEGLGRPVEIVSGGATSSLELVDNGQMPEGINNLR
                     VGEGILLGHNTSRMQAIPGLHRDAFVLEAEIVEVRTKPSQPIGLIGADAFGNVPVFED
                     RGRRLRAIAAVGKQDVKVDGLTPLDPGVVVLGASSDHLTLDVEDCPVRPALGQIVRFT
                     LDYGAMLALTTSSYVEKVYA"
BASE COUNT         3990 a         6756 c         6167 g         3635 t
ORIGIN      
        1 cagttccggt ccatgctgac ggaagagcag aaaaggatgc tggacgaggg ccccaggggc
       61 ttttgccccc gcaagcgctc ctggtgaacg ctttctgaag cggaggggcc ccggccatgg
      121 ccggggcccc tcttttgcga ccgtttcagc ccccgttctt cggaagaagc ttccggaacg
      181 tgacctcgag cgtttcgtgc tgcaccctcc tggcatcaaa ccccagcgaa cggaacacca
      241 cctggatgca aaagccgatg ccgaaggccg acagcactgt tccaaacccg accggaccgc
      301 ccagcagcca tcccaggagg acggcgcttc cctcgacgat tcccctgcac aggcccacgg
      361 ccagcccggt tttcctttcg agcgccacca tcaggctgtc ccggggtccc gccccgaaac
      421 ccgaccccat gtagaagtac gaggcaaggg agatgatgaa gaggccggca aacatcatga
      481 gaagccccgg gaggaatccc gtcatccggg gaatgatccc caaagccagg atgcggtcca
      541 tgaaaaaccc gatcaggacc atgttcagga tcgtgccgag tcccagtttc tccccggcca
      601 gaaagaccag cacgcagatc agaaggccgg ccaggatcga gacgttgccg atgctcaggc
      661 ccaccttgag gctgatcccc tggtgaaaga cctcccacgg gccgaaaccg aggttggcct
      721 tcatcgtcac gacgatgccc agggcaaaaa ggaacaggcc aaacaggagc ttccccatcc
      781 gcctggcaaa ctcgatgcgg ttcattctca aaacctcccg gttcaaaacc cggctccacg
      841 gaggagcgcc acggtgccga ccccgaggag cagggccaaa agaacgttgc cggttttcct
      901 cgtggcgacg aagatcacgc cggccgccac cccgcccggg atgccggccc ccgcgatgcc
      961 cggaaaaact agggccgcga aaatggcccc ggggagggcg tccatccccc gcttgacgcg
     1021 acccgttcgc ggcagcttct cgccgaagac caggccgagg aagcggatcc cccagacgag
     1081 cagggccgag aggcagatga cggcgattcc ctccatgtcc gaacagttca cgaggggtct
     1141 cctttccccg cggccgccgc gaacgcgccg gccaggcctc ccagcaggac gtaccaggcc
     1201 ccggggagga ccctgtgggc caaaagcgcc actccggcag aaacggccca cggaaggagg
     1261 tcgtgtttcc cccgccatag ggtcaccgcc agggcggtga agatggccgt aaaggcgaaa
     1321 tccattccga agcgctcggg atgacggatg gaaacgccca gcgcatgccc cgccagcgtt
     1381 ccgagcgacc aggtggccat gaccaggatc cccccgccca gcaggtagtt ggccccgatc
     1441 ggggatttcc ggcgttccgc catcgccaga gcccagttct cgtcggtaac gaagtggacc
     1501 ctccaggccc gctcgaacca gccgtagggc tccatcatgg gggcaacggc ggcggtcagc
     1561 aggaaatagc ggacgttcac caccgccgcg gccagggcca tctgccaggc cgaaacgccg
     1621 gtctgccaca ttcccacgag gacgaactgg gccgagccgg aaaaaaccgc caggttcatg
     1681 aaggccagcg ttttgatccc gatgcccttt ccggccacca ggattccgaa gaccagggca
     1741 taggcgacga tccccaggga aacggggatc gccgccccgg ctccccgggc cagttctcct
     1801 ttcaaaacgg cctagccctc cagcgccagg tccacggcca tgagagcgtg aagctccgtc
     1861 gagtcgaaga cgggaagatc cgtgtgctcc tgctggatca gaagggggat ctccgtgcag
     1921 gccaggagga tgccctcggc cccccggtcc gcgagttttc ccatgatcct gagatattcc
     1981 tcccgggagg attcgaggat cttcccggca cagagctcgt caaagatcac ccggttcacc
     2041 gtgacgcggt cctccacgtc ggggatgagc accgaaaggc cccacttccg ttccaggcgg
     2101 ccttttaccg tgtctgcctc catcagcgag cgggtcccca aaagcccgac ggtccgaaac
     2161 ccccgcttca ccacggccgc tcccatcgcg tcggcgatat ggatcacggg aacccccagc
     2221 agcctttcca cgtcctcggc aaaggcgttc gtcgtgttcg aacagatgag gacgaaatcc
     2281 acgccggacc ccttcagccg ggccgccgaa cggagcacca gctgccggta ggtctcgtgg
     2341 tctccggcgt tgatggcctt cgccaccggg tcgaaatcca gcgaggtcat cacgcactcg
     2401 gcggagtgca gcccgcccat ccgcctggcg acctcctcgt tgatgatgcg gtagtactcc
     2461 ttgctcgatt cccagctgac gccccccaca agcccgatcg tcttcatcgg cagtcctccc
     2521 tcctgaatct cagaaccccc tccagcaccg tgacggccga accgcccacc ttcaccctgg
     2581 agatttcccc atcccgcttt tccagcgaga cgaagaccct tcccggccga ttgacttcca
     2641 tgcccgcttc gctggcaagg tccaaccgtt ccgtgaagcc gaccaggccg tttcgcccga
     2701 tgaacgcccc aacgcttccg tttccggtcc cgcagaccgg gtcctcgtcg attcccacga
     2761 agggggcgaa ggcacgcatg tggagatccg ccccaggatc caccgtttcg aggcaaaacg
     2821 gaacaatccc gatcagcccg tggcgttcgc tgaacctccg gatttccgcc aggtccgggg
     2881 ccagggcctg gaccgccgta aggcgcctga atcccacgac aaaccaccaa agccccgtgg
     2941 agaccgccag gggctcgacg tccaggaatt ccgacgcttc cacccgaagg agcccggcca
     3001 cttcttcccg gctggcccct gtctcccgga agcggggctt tgcctgggtc atcgtgaaga
     3061 ccggccgatc gccccggaag gaccgctcca cctccaggac ccccgccttc gtctcctggg
     3121 taaacaccga ccgggcggca tcgagccggc cctcctcgtg aagggccgcg aaggtgccga
     3181 tcgtcgcatg cccgcagagg tccacctcgg agttcggcgt gaagaaacgg accctgaaat
     3241 ccgccttgtc cgatggagtg acgaaagccg tctcgctcag gttcatctcc cgggcgatct
     3301 tctgcatctg cccctcgtcc agcccttccg cggaggtcac cacccctgcc gggttcccgc
     3361 caaacgccac atccgtgaac gcgtccacct gcttgatccg aaccgtttcc accctgaaag
     3421 ccctcccctt ttctcagact cagacgatcg gttcctggcc ttcaaacggg ccgaattcac
     3481 cggaaccgtt ttcccgcgcc ttttccgccg cccgcgccag ccgacgcact ccttcctcga
     3541 tctcctcgag ccggacatgc gtaaaattca ggcggatccg gctctgcctg ggatgcccgt
     3601 cggcataaaa cgcggttccg gggagaaagg cgacccggtt ttccgcggcc ctcgccatga
     3661 aggccctcag ggggaaaaac cccggcaggc tgcaccagag gtagtagccg ccctccggca
     3721 ggtcccagtc ccagccgatc cgcgtccggt agcgggtaag gctttcagcc agacggtcgc
     3781 gtttttcccg gtaggccttc cggacccttt cgaggtgcgg accgtagcgg ccctccccga
     3841 gaaagcggtc caggaggatc tgggcgggag tgttcacgtg caggtccgtc atctgcttca
     3901 gcctggaaaa cctttcgatg accggtgccg gagccgaaac ccagcctacc ctgaacccga
     3961 gggaaaggac cttggaaaaa ctgctcaggt agatcacgat cccgcgccgg tccagggcct
     4021 tgagcggtgg aaccggccgc ccttcgtacc ggagctcccc gtaggggtca tcctccagca
     4081 ccggcacgcc gaaccggcct gccgtttcca ggagctcgcg ccgcctttcc agggacaggg
     4141 tgatgccggt cgggttctgg aaggtcggaa gggtgtagat cagcctgggg ttgtgacgcc
     4201 ggatggctcc ctcgaggagg tccgtcctca tgccgtccct gtccatcgga atccccgcca
     4261 cccgggcccc ggcggcccgg aagatctcca gggccccgaa aaaggtggat ttctccgcca
     4321 ggaccgtgtc cccgggagaa agaagggctc ttgccgcgaa atccaggccc tgctgggaac
     4381 cggaaaggat catcacctcc cgggaaggaa cgtcgatccc ccggtccgcg agaagctccg
     4441 caaccttttc cctcagcggg gcgaatccct caacggggct cggcaggaac tgggacctga
     4501 agccggaaat cccgagatcc cgctgaaggg ccaggaattc ttccaccggc aggaggtccg
     4561 gatccggaaa tcccggggcg aaggagatca cgccttccct cccgttcacg accatcatct
     4621 cgctgaagag atccccgcac gacgcggttt cgggcctcaa caggcagtcc cactccaggg
     4681 gctcggccct gccggattcg cgatcgagcc cggttccgcc cgcggccacc acggtccccc
     4741 tgccgaccct cgcctcgacg aagccgtcgg ccttcaactc gccgtaggcc ctcacgaccg
     4801 tgctgcggtt gaccccgagc cgcttcgcaa ggtccctttc cgggggaagc ggcgttccct
     4861 cgaggagcag tccgccgagg atccgttccc ggaacgcctc ggcgatctgt tcgaacaggg
     4921 gcgttcccga gtctctttcc agatgcagcc gaatcatctt ccaccccttc ttcttttccg
     4981 aaaatcggcc cgcctgacac catccaatca aggatcatac acccgatttt ttcccgtgga
     5041 acccccaatt ggccccctgc taacccatcc aattaaaccc caggcccaaa aagaaaggcc
     5101 ccgctcccgt ttgggaatgc ggggcaacct cgcggccgtt ctttccggcc ctttgctatt
     5161 cacccagcac tctcggccgg tgcacgtaga cgctgcaggt cgcgtgggcc accacctcgg
     5221 ccgccacgct tcccagaacg aaccgtttcc gttccgggtt tccccggtga ccgatcatga
     5281 tcaggtcggc ggccaccccg tcggcaaagt cgagaatcgc ttcggcggga aagccggggc
     5341 gaacggcaac ccgcagatcc tgaagcggct tgccgaactc cgccgcggcc gcgtgaaccc
     5401 agtcatggac ttccttcctg acttttgcca ggaggacctc gtcgatctcc ttcgaccggg
     5461 atcgccatcc cgtccgcagg ggttcgaccg catggaagaa atagagggtc acgtccttct
     5521 cacgggccgc caaatcaaac gcggcatcca tcaggcttcg gctgaccgcg ctgtggtcga
     5581 tcgcgacaac cactcttttc atgctttcac ctcccgggag gtacgggttc attttttatt
     5641 gactaattat ttattatatc actatgtttg tttttcttct tcttcttgcc cggcaagccc
     5701 aagagttgct gtagaatatc gggggtccag acccaaatga caggcgggga tgttcatgtg
     5761 caggatgctg ctggcaaaca gaaaagggat cgaggagctc aagctggccc tggccgcgat
     5821 cccgggttgt tccaccctcg aaaagtacct gcgggatctc gagctccgcg acgggggaca
     5881 gggcaacggg tacgcccttt tgaaggacgg tcgccttgtc cgcctcgaga agggcgtgaa
     5941 cctggccgtg ggaacgatcg cgaaagtcat ggaaacggag gattacgact gggtcctctt
     6001 ccacacgcgg gaagccagca cgggcagcgt ttccgacgcg aactgccacc ccttccgcgt
     6061 ttccggcaag gccgagctcg tagcggcggt caacggcaac gagaagtcgg tgatggaact
     6121 ggcccgtgcc gcaggaggaa tcacggacag cgaattcatc ctgaaactcg tcgccgagat
     6181 ggggcttccc ctgaaagaaa cccttgccat tttcgaatcc aacttcttcg gcttctacaa
     6241 cggcaggccc ttcgtcaaga aaggaaaccg ggacatgctc acctgggaaa agggggaagg
     6301 actcgtcttt gccagcgatt ttcccttcgg catggaaggg ctcaggaagc cggaaccgga
     6361 cttcttctgg atggagggaa gcaccggggc ctgagcccgg cgaaaatgtt ccggggcaca
     6421 cgagggaggc cttccggcct ccccttcttt tgccctcgaa tttcaacgtt tgtgtattct
     6481 tgacaatatt gttgatttgc cctagcctag atccgcacag agcctgaaag ggcgaatacc
     6541 aattcctgaa agggggccca cccatgcttt tcctgtctcg cgagcaaatc ctgtccttct
     6601 tcaccatgag ggacgcgatc gaggccgaca agaaagcctt cgtgctccac accgagggca
     6661 aggcgaaagt cccgcttcgc atcaacctgg aaaccgagga caagtccggc cagtgcatgt
     6721 tcatgccggc ctacgtgggc ggggagttga acatggccgg ggtcaagatg gtttccttct
     6781 tccccgggaa cgcgcaaaag ggaatcccgg tcgtccccgc gacggtggct ttgatcgacg
     6841 gcaccacggg actggtgacg gcgatcgtcg agggcaccac cctgacgcag cttcgaaccg
     6901 ccgccatttc cggggcggcc acggaactgc tctccaaccc cgacagccgg gtcgccgccc
     6961 tcttcgggac gggcggccag gctccggccc agctcgaggc cctcatgacc gtccggagcc
     7021 tgcgggaagt ccgcgtcttc gacgtggatc ccggccggat tcagaacttc gtcacgaaat
     7081 accggcccct ggcggatcgt ttcggcaccg cgctggtggg agcgaaaacc cccgacgagg
     7141 ccgttgaagg ggccgacgtg atcaccaccg tcacgacctc cggcaagccc gtgtttgacg
     7201 gaagccgcgt caagccgggg tgccacgtca acggcgtcgg ctcctatacc cccgtcatga
     7261 gggagctccc cctggaactg ctgaaccggg ccgggcggat cttcgtggac aaccgggaag
     7321 cggtgctggc cgaagcggga gacttcctca ttcccatggg agagggtcgg ttctcccccg
     7381 aaaggatcgc cggcgaactg ggcgacctga tcctgggaag ggtcccgggc aggacctctc
     7441 ccgaggaaat caccgtcatg aaaaccgtcg ggttcgccac gctggacgtc gttgccgccg
     7501 cggcgatcgt ggaaaaggcg aggggactgg gcatcggaag cgaaatcgcc ctttaaggac
     7561 ctccgtaccc gatcaacggg gcggacaatc gctgggaggg gatctccgtg ccaagggaat
     7621 gccatccgct gctcaaaccg ctggttcccg ttgttcgcct cctcggcgcc atgctgggcg
     7681 gggactgcga agtcgtcctc catgacgttt ccggggaggt ccccttcatc gtcgccatcg
     7741 aaaacgggaa cctgagcggg agggacatcg atgcccccct gaccgacctc ggccgtttcc
     7801 ttgtcaccag cccggaagcc gaaccggtgg atttcctggc caactatccc tcggaggggc
     7861 ccggcgggag ggccatgcgg tccggggtgg ccatgatccg cgacggcagc cgccggctcg
     7921 tgggtcttct ctgcttgaac ttcgacatga caaaagggtt ttttctaaaa gatctcggaa
     7981 cgttcctgac caccgtccgc cccctttcgt tcagcgcctt ccagtccgag gacttccggg
     8041 gaatcacggg agatccggcc cttaatctcc tggaaaaggc ccggcaggat ttcgggaaac
     8101 ccctccggta cctggacagg gaggaacgga tcgcctgcat ccgttttctg gaggaacagg
     8161 ggttcttcca gctcaagggc gcggtttccc tgctgaccaa ggaaaccggc aagagccgct
     8221 acacccttta cgcggacctc cgggccgtcc gctccgggaa ggaggaatga gaaccatggt
     8281 gaaaacagcg atccccgagg aaaagatccg ggaggccatc gctttccacg ggcactggtg
     8341 ccccgggctc gccacgggca tccgcgtggc ggagctggcc ctccgggagg tcggccgggc
     8401 cggggacgag gacatcgtgg ccgttgccga atcggacacc tgcgccgtgg acgcgatcca
     8461 ggtgctgacc ggatgcacgg tgggcaaggg gaacctggtc ctcctgggca tcggcaagat
     8521 gggcttcagc ttttaccgca ggcgggacgg caaggccgtc cggatcgtcg cccggcccca
     8581 gaagcccggg gaaggatcgg acgagtacag gcggctccag gcgaagagca acgacggtac
     8641 cctgtcgggc gaagaagccc ttcgtttcga aaccctccgc caggaacggg gcaggcggat
     8701 tctcgaggcg gacctggacg agctgttcga gatcaagaaa cctctccggc ccgtgcctcc
     8761 ccacgccccg atgaagcgca gcctcacctg cgaagcctgc ggggaatccg tcatggaaac
     8821 gaaggcccga ctgtccgacg gacgggtgct ctgcccggac tgtttcgagc gcgaggtgcc
     8881 gcgcccctga gaaccacgcc accatctgaa accagggggg gaggagcctt taggctcctc
     8941 ccccccccat ggtttcagaa cgtttatttt ttcgtctctt cgttcctagt tcagccccat
     9001 cagcaccgcg ccgaccacca ccagtgagcc gaccgcaaac agcagcccct ggaacttgat
     9061 ctggaacttt gcccaggtgg tccagtcgag cctcgccgcc cccaggcagg ccatgagcac
     9121 ccccgaggtg gggacgatga ggttggtgaa accgtcgccg agctggaagg ccagcaccgc
     9181 cacctgccgc gtgacgcccg ccaggtcggc caggggagcc atgatcggca tggtgagggc
     9241 cgcctggccg gatcccgaaa ccacgaagaa gttgaacacc gactggaaca ggtacatgaa
     9301 ccaggccgaa acggctgggg tccagcccgc gatggcgttc ccgatggcat gaagaaccgt
     9361 gttcatcacg gtggcgtcgg tgggggaatc gccgcccagg atgaggacga tgcccttggc
     9421 catgcccacc accatggccg ccccgacgag gtccttcgcc ccgtcccgga agctcgacgc
     9481 gatgtcgttc accgtcatgc cgttcagctt gaaggccacc ccgatgaggc ccgagacgat
     9541 ccccatggtg aagaactggg tcgcgatctc ggggatgtag taccccttct ccacgacgcc
     9601 ccagatgacc cagacgatgc ccgcggcgag ggtcagcagg accagggaat gacccgtgcc
     9661 gaaatggctt tccactgcct tgttcttttc caggtcctca cggaagaagg catcggactt
     9721 gtaggaaatc gatcgggtcg gctcggcctt gacccgcttg gcgtagaacc acgtgaaaac
     9781 gatgcccagc agggtgaagc ccgcccacat ggcgatccgg aaccccgagg ccgaaaggac
     9841 cgggatctgg gagattccct gggcgatggc cacgctgaac gggttcatcc aggacgtggc
     9901 aaaaccgatc tgcgtcgcca cgtagctcga gaacagggcg gtgacggaat cgtagcccat
     9961 ggcgacgaaa agcgggcaga ggatcatgac gaagggaatg gcctcttccc ccatgccgaa
    10021 gaccgcgccg cctagggaga agagaacgaa gaggatgggg atgatggccg cttccatccc
    10081 cctggcccgg gagatcatct tcagcagccc ggactccacg gcccccgttc ggaggatgat
    10141 gccgaaagcc ccaccgatga ccaggatgaa ggccatgacc ccgatggccg aaccccactt
    10201 gctgcccgag acaaagccct cgaacacgta gttcaggatg cccatgtccc cgttgggggc
    10261 gaagacgggc atgccctttt tcaccgggtt cccctggtcg tccgtggcga gcttgaacgt
    10321 ttcggggatg aggaccgtcc gcgttttcgt ctgctcgccc atcttgtagg aaatttcgtg
    10381 ggtctggtag ctccccacgg ggatgaagta ggtcaacaaa gccgcaagca ggacgacgaa
    10441 gaagatgatg atgtaggtat ctggaactcg tgtcgccttc ggggcgacgg gcgcctggtt
    10501 cgtttccggc atttcctttc ccctcttttc cgatggattt ttccggctgg aatcctcgcc
    10561 ctcctcgccg ctatacttcc tgagaaaggg cttgtttcct cgcggaacga gccggacttt
    10621 acacaatgga tccattatag cataatggat ccattgtttg aaaaggggat gaccgaattg
    10681 aaccgcccgc cgaccgatcg ccctttccag gccttcgagg tcgttttctg gcacgtcctg
    10741 gacgagatgt tcgaggaacg gctgctcccg ggggatgtcc tttacgaaac ggccctgtcc
    10801 caggagctcg gcctgagccg gacgccggtg agagaggccc tttcccgcct cgtggcggag
    10861 gggttgctgg aaaaccccgt ggggacccgc ggataccgcg tcccggatct ctcggtagaa
    10921 gacatgcagc aggtcttcca ggcccgggaa atcctcgaat ccagcgcggc ggccctggcc
    10981 gccgagcatg cgcgaaaggg agacatcggc gaactggagg agatccaccg gcgcgaaatc
    11041 ctcttttcca gccaggaaaa ccgaagggag tactgccggg ccaacgtgga attccacttc
    11101 gccgtgatgc gtatcggcgg aaatcgctat ctcgagcggg ccttccggcc ggttttctgg
    11161 agaagccagc tgtacgtcaa ctacctggcc gagttccacc ccctgaacgg ggaggaacga
    11221 accgcggaaa gcggtcacaa ctcgcccgcg gagcatgccg ccctgatccg ggcgatcgcc
    11281 aggagggcgc cgcgggccgc cgcgaaggcc gccgccgacc accttcgctc cacgagggcc
    11341 taccggatcc tcctggacac ggagagggcc cggcacattt tcggggggag ctcgcgctga
    11401 agcttccccg tccaccttgg agggattgga tgttcctgct tgttgaaaac gccgaaatct
    11461 acgcccctga aagactggaa gcgcgggagg ttttcgtggc cggggaacgg ttcgcctgga
    11521 tcgggaacgg cttcccggca aaatccaccc tgcccgggat cgagcggatc gacggaaccg
    11581 ggtgtctcct gctacccggc tttatcgaca accacgttca catcatcggg ggaggaggcg
    11641 aaggaagcta ccggacccgg acgcccgaaa tctccctgac ggacctgacg accgccgggg
    11701 tcacctcggt tgtgggcgtg ctggggaccg atgccgtcac ccgccacacc gcgagcctca
    11761 tcgccaaggc ccgcgccctc gaggaagagg ggatttcggc ctgggccatg ctcggctcct
    11821 accagcttcc ggtccgcacc ctcaccggga acatccagga cgatatcgtt ctggtggaca
    11881 agctcatcgg cgtcggggag gtggcccttt ccgaccaccg ctcctcccag cccctccagg
    11941 aggagatcgc gcgcatcgcc gcggcggcaa gggtcgccgg gatgctgtcg ggcaagggcg
    12001 gcatcgtcaa cgtccacatg ggggacggac cgcggggcct ttccatgatc aaggctgtcg
    12061 cacaggaaac cgagctgacc ctggcacagt tcttgcccac ccacgtcaac cgcaaccccg
    12121 aactgttcgc agaggcgatc gaatatgcgg cctccggggg acacgtggac ctcaccacca
    12181 gcaccacccc cgttttcctg gcggaagggg aggtcaagtg cagcgtcgga ctgaagcgcc
    12241 tgctggatgc cggcgttccg gcaagccggg tttccttcag ctcggacggg cagggcagca
    12301 tgcccgattt cgacgagaaa ggacacttca ggggcctcac cgtcggccgg tgctcctccc
    12361 tctggggaga ggtcgtcgac gcggtcaggg acgaggggat ccccctcgaa acggccctgc
    12421 aggtgatcac gtcgtctccg gcggaccatt accgcctgcc gggaaaggga cacgtgacag
    12481 cgggagcgga cgccgacttc gtgctggtcg acagggaaac catggcgatc cgcacggttg
    12541 tcgcccgcgg gcaggtcatg gtacgggacg gccgtccggt cgtgttcggg accttcgaaa
    12601 agcgcgaaca ataaaaccag ccgtcacttc ccatccaatg ttgttataaa accattaacc
    12661 caagatggcc ttttaaataa aggctgtctt gggttttcat gcatacttga caaggttcac
    12721 ggccggtcct aagatttacc gcaactttag tgtcgttgac cgatgaagat tcagggaggt
    12781 gagtcccctt ggaaaaccga ttctccagaa agattggtgc cggctggttc atttattttg
    12841 gctactccga cgggacccct cctgagtccg gcggctgcta gccgtattca gacgcaggaa
    12901 gggccccgcc gagcgaaacg gcggggccct ttttttatcc aggggggatg cggttgggcg
    12961 aaaagagcgg gattttggtt tgaatggacc caagagaaaa gaacagggga gaggagaatg
    13021 ccaaatgaca gaagagaaaa aaagcagcat cctgactttc tttatgaact acggctggta
    13081 caggaaatac ctgctgcccg gcttcatagc ccaatcggtg ctgatcgccg gggggtacgg
    13141 cacgggacgg gagctggtcg agtatttcgt ccaatacggt cccacgggcg gactgatggg
    13201 catggggctg accacgatcc tctgggctgc gctcttcgct ctcagcttcg agtttgcccg
    13261 cgccttcaaa gcctacgatt accgaacgtt cttcgaaaag ctcatcggtc ccggctggat
    13321 ggtgtacgaa gccgccttca tcgttctcct tttcctcatc atgggggtcg tcggagccgc
    13381 ttccggcagc atcctcaggg attccttcgg cctgccgcct ctcgtcggct cggggctgtt
    13441 cctcgtcttc gtcgcctacc tcacctatag cggcagcaag gccatcgagg tcgccctttc
    13501 ctggtggtcc tacgtccttt acgcggttta catcctgttc ctctatttcg ggatctcccg
    13561 cttcggggat gccatttccg cgaactttgc agcccacgtg gtgaagcccg gatgggccat
    13621 gggaggattc aagtacgcct tctacaacat cgccgtcgtg tctaccgttc tcttctccct
    13681 gaactacctg gaaacccgaa cggaagcgat cctttccggc atcatggcct ccctgatctg
    13741 catcctcccc gcgggcctct tctacgtcgt cacggtcggc ttctacccgg gcgtcctgaa
    13801 catggagatc ccggccaacg gcatcatcgc gaacctggga gtcaggttcc tcctgccggt
    13861 ctggctgatc gtccttttcg gcacgatgat cgagacgggt gtcgggttct tccactccat
    13921 caacgaacgg atcaactcga ctctcgtggc aaaaaggggc aggggcatga gcaacctcgc
    13981 gcggggcgcc gtgggggcgg tcctggccct gatggggctg gcgatttcga atttcggcct
    14041 catcggcctg atcgccaagg ggtacgggac catcagctgg gtgttcttca tcctgcaggg
    14101 cgtcgcgctc ttcaccatcg gtctctacaa gctgagccgc caatccagcg ggaaaaccgc
    14161 ctgaccttcc ctttcgtgtt cgtggaaaac taaaaaagca acggccctcc cgggctttcc
    14221 gggagggccg ttgcctcctc aggcgtttgc cagccgctcc aggagagcct cgagctttgc
    14281 cggcgcagga acccgaaggc gaaccgaacg gtcgtccagg ccgaaaaagc cgcttcctcc
    14341 ttcgcaggag acgcccacgg cggccaggcg cctcaccagg ttgccggacg cctgggacag
    14401 aagaaggatc gggacgcgtt cgtccgtgtc cgccacgaac cagcccggct tcttcgaaac
    14461 cgcgtccagg atgcgcgcct tggctgccct cacgtaggcc cgggtttcct gcagcggggc
    14521 cggatccttc aggatctcaa gggccatgcg cacgtccagc gtcccgatca cgaacggggg
    14581 ctggaccttc cggaacagcg ccgccagatc cggatcccgg cagaccgcga agcccgcgcg
    14641 aatccccgcc gcaccgggcc ccttcgaaaa ggaccggcag gtgacgaggt tcgggaaatc
    14701 cagaacggcc gccgattcgt cgtcggggag gaaatccccg taagcctcgt cggagatcac
    14761 ccaggtgccc gcctccatcg cgaagcgggc cagccgggcg acgtcctcca gggacatcac
    14821 ctggccggtg ggattgtgag gccggtcaag gtacagcacg tccggtttct tctcgagggc
    14881 ctcgagaaca aaagcggtat cgatcgtgaa gcgcggcgga accagccgga gcggttcgta
    14941 cgaggccccg ttgtagagag cctggagaac cgggtcggtg aactgcgggg aaaccccgga
    15001 caggaccgaa ccgggacgaa ggaggatccg aaagaggctc accaggaggc cgatggaacc
    15061 gccccccacg agaagctggt ccggggaaac cttccaggcg tcgtgaaccg ttgcgatcgc
    15121 ttgttttaag ccttccggct ccggctcggg atagtcgcag aggtcgcaag gaatcccttt
    15181 ttccaggaag cggcggaggc tttccggcat gcccagggga ttcgtcccca ggccgcagtc
    15241 gatcacctcg attccctcgg ggtagaccac cggctcagcc gcggcgtacc cctgctgggc
    15301 gatctcgaaa atctccctcg gaagtttccc tctccacatc tgtcttcccc ccatccgtat
    15361 cgccttgcca ggcccttctt ccgattttgc cccgatccgg gtttgctccc gctcttgccc
    15421 cgggcccctc tcgatgaaat aatccggaca ttcgttccac catttctgaa aggggtgact
    15481 cccgatgaaa aaatggctcg tttcggccct cgcttttgcc ctgctggcct ttttcccggt
    15541 gccgagctgg accgtagaac ccccgggcta cgtggtttac caggttgtgc ctacctactt
    15601 taacacgctc gaagaggcca tggccgcctt ctcccagatc cggagccaga tctccatttc
    15661 ggggggagcc atccaggggc tccacctctc aagggaagcc ctgtccttca aaaccgctat
    15721 gggatctccc ctgtcgatcc cgtttgcttc cttcaccctc cttcgacttt tggagattcc
    15781 caaggcgccg gatccatgga agtgggccct ggccgcgaag gtttccgaaa agggaaatcc
    15841 ggttttcatc tcagccgcat cccgcgatgc cgccgagaaa atgttctcca tcctgtggag
    15901 cttcgccgcc gcagcgggag ccccgctcga gcttcgggac gtgggagcgg ccgtcgtcga
    15961 cccaaccccg caggacctga agatcaacgg cctgaaagag ccgaagggcg gggttgtcct
    16021 gtgggtctcg gtcggcgggg catcggaaag ggcagggctc cagcacggcg acctgatcct
    16081 ctccatcaac ggtcttcccg tggacaacaa cacggagttc caggaaaagg tctggcccac
    16141 catcgctccg agcaggggcg tggagagcta cgacctggaa atcctgcgga agggcgcccc
    16201 gatgcccctc aagatcaagc tcccctcgct ggaaacactc cccaggccgc ccgcaacgct
    16261 ttccttcact ccgccgccgg caggccccgc cgccttccaa aaacagccgc cgaaactcgg
    16321 tttctccctc aggagcctcg aaccggctga aaatgccgcc ctcaacggaa agagcggggc
    16381 cgtcatcgcc gagctgacgc cgggcgggga ggcggaaaag gccgggatgc aggtcggaga
    16441 catcctcgtg gcctgcaacg gccagcccgt gcccggaccg aaggaactcg gcggccttct
    16501 cgctttaggg gagaacacct tcaccgtgct tcggaaagga ttgcagctaa ccttcaggct
    16561 caacccgccc gtggcaagct actaggaagc ggccggttcc cgcgggagac gaaaggagcc
    16621 ttcaaaaaga aagggattca gaaacaatgc gatctgctaa caggtggatt tggtagtata
    16681 atattttggt tagaaagatt ccttctccgt ctttttcttt cctgtagttt gattatcaaa
    16741 aaatacgttc aggaaggagg gagcggatca tgaaaaaggt cgtcgtcgcc atcgacggca
    16801 gcgaggaaag caagggggtc atcgattacg cggttcatta cgccaaccgc gaagcagacg
    16861 cggagatgca tttcctccac gtgatcggga cggaggaaag gggcagggtc ttcgtcgagg
    16921 gatacgcggt ggaactgccg acggtcggcg aaagggtgaa gcaggatttc gaggagttcg
    16981 tccgggcaca gatcgccaag agcggaagga ccattccgcg gatgtcggtc acggtccttc
    17041 ccggcatccc ctacgagcag atcgtcaagt ttgccgagga aaacgatgcc gacatcatca
    17101 tgatcgggca tcgcgggttg agcaacctga aacgcttctt cctcgggagc gtcgccgcca
    17161 aggtggtcgc ccacgctccc tgcagcgtct acgtgcaccg gccgaagggc aggcaggcct
    17221 gagacggtgt cggctaaaaa aggggccccc gtcagggggc cccttttttg ggatacccga
    17281 accgccccgc ctcagggatt cgccgggttt tcaaggatcc cgacgaaaag agggatcccc
    17341 gccttcaggt ccacgatgcc gtagaggaac ggccggtcaa aggagatctt ccggtcaacc
    17401 gagggcaggc tcgtcatccg catgcccacc gaggtcacgg ctgaagcctc ggtgcctttc
    17461 tcgtcgacgc ggacgaaggt cttgtgccgc acggtcccga tgaagagatc cttcgtgcgc
    17521 cgctcgctca tcgccgaaaa atccgcccgg gcgggatcaa aggcgatgcc catcccgagt
    17581 ttcttcagct cgtcctccag cgtatcctcg tagcgcgact cgaacttcgg cagcgccagc
    17641 tgaacttcca gggccggctt ttctgccatc cgccgggcga ttccctcgaa aagggttgcc
    17701 gggtcctgcc gggcaagcca ggccctcggt gcagtttttt cttccggcag cagggcaaag
    17761 aaggcaaaac gcccgtcctc gtaggggagg cagacccccg tggctccctt cagctggaaa
    17821 acggtcatcc ggccggtccg gtgcatgaaa tcggtctcga tgtcaccgcc cggagcgtgg
    17881 aaaacctcct tgcgggtttc gcttttctcg aacggcacct gccagtccga cttgaaatag
    17941 accgcgttga ccaaaaagag cacctcgtcc cggcggatct cctcgaggat cctgtcgatc
    18001 gtcccgcgcg tggcggtttt cacccaggcg ttcacgatcc caggggcgcc cctgtcccgg
    18061 aaatcgatcc tgcggatctc ggcaccgaaa aagtccctgt tgcgctgcag gaaaaatctg
    18121 gaaggaacgt atcccttgtc gaaccagatc gagttggcga tccccagggt cgttttgccc
    18181 cccttgcggc tcaccccgct cgtccaggcc cggatcgacc ggttcaggcg gtccagcgtc
    18241 agtccatctc ccgccagccc tttcagcatg gccgccttcg tggaaccctc ggccccgttc
    18301 gccgccatgg tcagggccag gtgaaccgaa accggggaaa tcatgaggtt gcccggttga
    18361 ttttcagcgg agagccgcag cagcccggcc gcaaaccggt tgaccgcttt tcccaccgct
    18421 tcttccggca caaccggccc cgtggagcac ccttccgccg ggaccgtggc catggccggg
    18481 atcccggaaa aggtgccgca caggcaaaaa cccgcgagca gcaaaacgaa aagattcctc
    18541 ttcaagaaat ccacctcctc ttctcgagga caggcaatgc aaaaaccttt cgccttgatt
    18601 tcattttatc cctcgaaaag ccgtcccgta tccgcctgaa tacggaaaga agcagcccct
    18661 cgcccgcgtt gaattatcga tgataaagga ataccatggc aatatcctgc cacgtcatgc
    18721 taatgggggg gttgatgaaa tggccaagct gcagaaaatc gagggaatcg gagaggcgta
    18781 cgcgaagaaa ttggagggag ccggcatcac cacgacggaa ggcctgctcg aaaagggagc
    18841 gaccccgaag gggcgcgagg aattggccaa gatgacgaaa atcccggcca gccagatcct
    18901 gaagtgggtc aaccacgtgg acctcttccg cgtcaaggga atcggcgcgc aatacgccga
    18961 actgctggag gctgccgggg tcgacacggt cccggagctc gcgcaaagaa aacccgatca
    19021 ccttctccag aagatgatcg aggtgaacga gaaatcgaag aaagttcgcc agctgccctc
    19081 gatcaaccgg gtgaaggatt gggtcgagca ggcaaagaaa ctcccgaggg tcgtcaccta
    19141 ctagaggcag ggccggatcc tggatgtccg ggatccggcc cttgtttttc aggcgtagac
    19201 cttttccacg taggaagacg tggtcaaggc gagcatggcc ccgtaatcca gcgtgaaacg
    19261 cacgatttgg cccagggccg ggcggacggg gcaatcctct acgtccaggg tcaggtggtc
    19321 actagaggcc ccgagcacca cgaccccggg atcgagggga gtcaggccgt ccaccttcac
    19381 gtcctgcttg cccacggcgg caatggcccg cagccgccgt ccccggtcct cgaaaaccgg
    19441 gacgttcccg aaggcgtccg ccccgatgag cccgatcggc tggctcggct tggtgcgcac
    19501 ctccacgatt tccgcttcca ggacgaaggc gtcccggtga aggcccggga tcgcctgcat
    19561 ccggctggtg ttgtgtccca gcaggatccc ttccccgacc cggagattgt tgatcccctc
    19621 gggcatctgc ccgttatcga ccagttcaag ggaagaagtg gccccgcccg aaacgatctc
    19681 cacaggtcgg cccagcccct ccgacaggcg atttcggcac tcgatcagca ggttcatgtt
    19741 ctccggggtg gggagcaccc ccccgaaaca gccgatgttc gtccccacgc ccatgcagcg
    19801 gattcgccgg caggaggcca gcgtcttgcc catggcttcc atctcgtccg gccagagtcc
    19861 ctcgcggagg tcacccacgt ccgccatgac catcacgtcc acggacttcc cggcggcctc
    19921 gcaggcctcc tcgagggcga gaacggtctc cggcatcgag accaccgtcc cgtccgccac
    19981 ctggaccagg tcctttagct ccgacagcat ggggatccgc agcagcatga ggggttcggt
    20041 gaaacctgcc tcccgcagcc gccgcaggtt tttcaaccgg ctgtccatca ggaggctgca
    20101 gcccccttcc agcatggccc gggccaccac cgggtccgcg cagaacccct tcgtcactcc
    20161 gaagaccgag atccccagct ttgccgcccg ccggaccacc tccgccgcgt tctcccggac
    20221 ctttcccgca tccatcacga gcctcggata cacgccatcg cccccccatc gagggggtca
    20281 gccccctctt cttattaaag gtcttcgtcg agcgttatca tacgtcttct ttcggggggg
    20341 cgccattgca ggcaagaacg ctcgtttaac gaacccgccc cggctgactg agccggggcg
    20401 ggttcgcata gaccgggcga gagcgtttga atccgcccta cttcgtctct ttcgagccgt
    20461 acctcctgtt ccattccttc aggatccggt cgcggttctc tgcggcccag gaaaagtcgt
    20521 tcttgatcag ctgcttcagg ggcttggc
//
LOCUS       DAOOVN010000017        19351 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_1920_length_19351_cov_14.708644, whole genome shotgun
            sequence.
ACCESSION   DAOOVN010000017 DAOOVN010000000
VERSION     DAOOVN010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 19351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19351
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1920_length_19351_cov_14.708644"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..1334)
                     /locus_tag="PLY83_03070"
     CDS             complement(63..1334)
                     /locus_tag="PLY83_03070"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOV29052.1"
                     /translation="MVQPSRILIAMVVSMFFGYMPWYGFSAVSSHLVREFGLTPQQVG
                     AILSAFQLGYVVTVIASGILADRFGNKRVLVWATLATAVFSTFFPFLSTGFKSIFALR
                     LATGLSAGAIYAPGMALLAAWFPPQRRGMAIGAYTAALTASYAGGYLVASPIAAAFSW
                     RLGILCISAPVFLAAFILCFFVTDAPSASPRPSETKKACRGGRPAWFPGGIKAPLLLT
                     LGYFGHMWELYAFWGWVGPFLVACALQAGWTVPRAESFSGVSSSCIILSGSLAVAGIG
                     MLSDRIGREKTILLSGLASGAAEFLFGFLHGAPLPVTLGVGSWIGFWSVADSAVYKAG
                     LSETVSPACRGTALGLQSAIGYLATVISPLLFGWILGTEAFVLGERARWGLAFSILGT
                     GALLAPAAMFLFPERGKGWNRKEFLGSSNRD"
     gene            1680..2945
                     /gene="trpB"
                     /locus_tag="PLY83_03075"
     CDS             1680..2945
                     /gene="trpB"
                     /locus_tag="PLY83_03075"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOV29053.1"
                     /translation="MRQASTLQRNARGTYGEYGGRFVPDSIVGLLEELECAFDSAMAD
                     PSFVSEMDRLLGEYVGRPSPITECANLSRELKGGRLFLKREDLNHTGAHKINNALGQA
                     LLAKRMGKSRLIAETGAGMHGTASATAAALLGMSCTVYMGAVDVARQARNVSRMKALG
                     ATVVAVREGQGTLKEAVDAALAAWVQDPGAFYLLGSVVGPAPYPEIVRTFQSVIGREA
                     RRQFLERVGRLPEEVAACVGGGSNAMGLFSGFLEDTQVILTGVEPGGKGLEKGLGFHA
                     ATLCAGNRGVVHGFRTVVLSDALGEPAPVHSIAAGLDYPGVGPELAHLRDTGRARYVA
                     VTDEEALDAFRRLCRSEGIIPALESAHAVAYALKRLPEMEPHQSLLVNLSGRGDKDLD
                     TVFPEKETNHFPVGAGGLNGIQSRGLFYV"
     gene            complement(2908..3720)
                     /locus_tag="PLY83_03080"
     CDS             complement(2908..3720)
                     /locus_tag="PLY83_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOV29054.1"
                     /translation="MRLSIGIDLGSMGIKAVLFDGSVRDRIMVPTGWNPKETGEQALR
                     EIFARNGIDPSDSPPLVLTGYGRNSLGKAGPTVTEITCHAAGSAFLFPGAETVLDIGG
                     QDSKAISLGRGGTVCDFLMNDKCAAGTGRFLQVMATLLDMELDDFSRLPEDVPPQPIS
                     SMCTVFAESEVVSLLARGVDKHSLALGLLDSIAGRACAMLQKLGGSGPVAFTGGVSRS
                     RNLVRLLEKRLGRPVVTSLDAPYAGAIGAALIAQKAEEAAPRRKTTPSTGSR"
     gene            complement(3717..3938)
                     /locus_tag="PLY83_03085"
     CDS             complement(3717..3938)
                     /locus_tag="PLY83_03085"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HOV29055.1"
                     /translation="MVFAYALFKTATDGMILHRRLRELGVKDDICPTPRHLADSCGIS
                     LRFDAIAAGAVREEAGRLGIPIELVVQRP"
     gene            complement(3997..5277)
                     /locus_tag="PLY83_03090"
     CDS             complement(3997..5277)
                     /locus_tag="PLY83_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HOV29056.1"
                     /translation="MADYHKMWEGLGMDLATHDQLCAALPPAFEQVFLTQKDRPQGMD
                     YFNMVIAEVHGLRIQELLDHKARGGKIIGSFCIYVPEEIVLALDGIMVGLCAGSEFWV
                     PAGEQVLPRNLCPLIKAGIGAKVSRTCPYFQSVDLVVGENTCDGKKKAWEVLGDIAPT
                     YVMDLPNTKTAAGFALFRKSMADLVAKLEEVTGNRLTFDGLLSAIETVDRKRAVLQRL
                     YETRKAPKVPISGLDMLLASQVAFYDDASRFISQAAALAEECEERIKSGKTVFPEGTK
                     RLLVTGTPIVVPNWKVHKIIETSGAAVVVEENCTGTRYFESRVDMSKASTVEELVDAI
                     THRYFDRIHCACFTPNEGRADDVVRLAREYRADGVVGLNLSFCTTYQVENEKVRKRLQ
                     SEGIPFLALETDYASGDEGQLRTRIEAFVESLRD"
     gene            complement(5608..6495)
                     /locus_tag="PLY83_03095"
     CDS             complement(5608..6495)
                     /locus_tag="PLY83_03095"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOV29057.1"
                     /translation="MRFLSRLTLRTRRTLKREAPGFLRVTVGTVLYSLSISLFVLPYR
                     FPSTGFTGISILLNYSLGLPVGLMTLIFNIGLFALGWRVLPKRFLYLTAYSVLLFSGS
                     LDLFRMFPSLHLSDKLLAVLTAAAINGISGALVFNAGGSMGGTDILAAIVRRKTGMEI
                     GKFTFWINSFVLVPAIFIVGIENTLYAVAMLFASSTFLDGALRSFDRCVQVFIISEKH
                     LDIRRFILEELRRGASVIHSEGAYTGKERPIVMSLLLPRQVADLKLFLAEHDPNAFLI
                     QTDATEVFGKGFKHWKATA"
     gene            6662..8581
                     /gene="htpG"
                     /locus_tag="PLY83_03100"
     CDS             6662..8581
                     /gene="htpG"
                     /locus_tag="PLY83_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOV29058.1"
                     /translation="MSETRSKEHLDFQAEAKQVLDLMIHAVYSNKDVFLRELVSNASD
                     ALDKLRLESLTNEALRDAVQSPAINLERDAEKRTLSVSDNGIGMTKDEMIRYLGTIAK
                     SGTKEFLRLLKEAGRGGDPEALIGQFGVGFYSSFMVADRVTVISRKAGTDEAWKWESE
                     GDGGFTLEPAERDSFGTTVTLHLRPADEEDGLRDYTDEWVLKEIVRKYSDFVAYPIRL
                     GGETLNSMKAIWARPKSEVKDTDYDEFYRHISHDWNPPLKRIAWKAEGTVTFDALLFI
                     PSKAPLDLFIRDVDRGLQLYVKRVFIKGDCRELLPDYLRFIRGVVDSEDLSLNMSREI
                     LQQNRQMELIQTGLTRRVLSALDEMRTTDPQSYRTFWKEFGRVLKEGLFLDGKNREKI
                     LELCLFKSSISGDEWVSLEEAASRVTGDRKEIFYLLGEDRSVLEKSPVLEAFRSRGIE
                     VLFMTDPVDEIWLQAVDGFRDYRLRDAARGDLDLESPSEKETGRKERDEAEKRLRPLM
                     DYLKNRLASSIREVRLSGRLTDSPACLVQDERDPSPQFAKVLASLGREAPQTQRILEI
                     NPAHPIIAGMAKMLEEAPASPTLEAYAELLYGIAALAEGSLPQDAPGFSRKLADFLRE
                     KVDSGNDSRKDVLPE"
     gene            8663..8830
                     /locus_tag="PLY83_03105"
     CDS             8663..8830
                     /locus_tag="PLY83_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29059.1"
                     /translation="MLTNRHVECPHCGTEFNVYLNYKEGKLVCPGCSKNFVPEYFNLD
                     YKQLEEEAEKK"
     gene            complement(8900..10174)
                     /locus_tag="PLY83_03110"
     CDS             complement(8900..10174)
                     /locus_tag="PLY83_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HOV29060.1"
                     /translation="MSATQWIALGLFIFTYSLIVTERMHRLKAALVGLSGILLLRLVE
                     QHDAFSFIDFNTIGLLIGMMIIVGIVKQTGLIQYTALIAIRQSRGNPWRLLVSLSLLT
                     AVVSAFLDNVTTILLIGPVALAVCETLDLDPMPFIFAEVFASNIGGTATLIGDPPNIL
                     IGSAAGLSFNDFLLNVGPAAALALLAMYLGLYVFYARDLSQNRPIEDLIEGFHTLRKH
                     LNRPLAARILTVLALVLAAFFLHGRLHLEAATIALAGATLGLVVCPVKVETALEEIDW
                     VTILFFSTLFMLVGTLEHLDIIGLAARFLANAAGDHPKILAMFLVWGSGTISAVVDNV
                     PYAAAVIPLVKDMASLAHLDARPLWWALSLGACLGGNATLVGASANLVMAGLAEKAGR
                     PLTFGNFLRKGIPITLLTLAASTAYVYLRYYL"
     gene            complement(10479..11432)
                     /locus_tag="PLY83_03115"
     CDS             complement(10479..11432)
                     /locus_tag="PLY83_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29061.1"
                     /translation="MNFEFNTLTLLILFGIGAVGLLFCHSRISEAAREIRETSSRLQE
                     NLDRRITETHDRFSLETRGLRDSVMSLNERMTRIQDEVTDTMEEARKRILQEAESRKD
                     DQEKRLTFFGNLVESTRKELLNGLEEKSRRLAEQGEQALETVRPLASEVSRLASLTRD
                     LPPDVAERLLALEKLYREQTIKLSAVEEMVLRERKDIQGLSSDLVAQFAALERDLRNH
                     FENRLNEFYQQVGGALQKTDDLDRQIRALSETIKESIRENIQLATGRIFALIGKASEE
                     AFSSEDALSLAGFRAPAPPKTAEPKAGESSKAKRYPYLFRK"
     gene            11559..12836
                     /locus_tag="PLY83_03120"
     CDS             11559..12836
                     /locus_tag="PLY83_03120"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOV29062.1"
                     /translation="MNRYPGKILLHVLFGFLVTAYFLSFFFRVSAAVVLPRLASEIGM
                     TAAMTGFISSLYYYAYAFVQPLCGSLNDRWGPLRVVGTGLSVAAAGSAIFMFASTPAT
                     LAAGRLLTGLGLAPMFSGALVYQSTAFPPRRYAFYSSVTIAVGNLGAVVSVGPLGSAI
                     DRWGRGPVFLFLSALSLFMAGLLFLNAKSDPVRGSNQGGRGPKTPFSAHLKTGFSTLW
                     TSRECRCLTLLWGISVAAMLAFQGLWSVAWYQTAYGVEAGIARNWAGAVGIGIMTGTL
                     LGGRFSSGPGGRMKAIGWGLSMNLGAWIALWLGIALRLPLTVTGSLGFLLGGATGFLV
                     VHFASALNEMTPRERAGAVLGVVNMLVMVSVIAFQWGTGAILARFPGAAAGSSSPRGF
                     LVTFGIVIALVAASSLAFRGMRRFPEAVRRGSE"
     gene            12833..14152
                     /locus_tag="PLY83_03125"
     CDS             12833..14152
                     /locus_tag="PLY83_03125"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOV29063.1"
                     /translation="MKRRTVFLLALAFACFSLVRADAGVIRSEKADEWGGMAGLGKKT
                     FRFVVLGDTQPLGPGRSLSPLVERLLKEISWLGPDFVVHLGDSISGYGDSLEEARTEF
                     SALKEKIDRLLPAVPFFAVPGNHDQATPGALSAYRETFGSLLYYDFPYGNARFVILNG
                     NFPAWMAPKGKDYGFFNLNDGMHVLPMVDWANEAFSRGGKMLFVFLHTPPFPAYTPPP
                     GKGTHGFLTESNRDAFVALLPKGRTKAVFSGHEHLFYVEERNGIKFFTLGTGGGPLSG
                     PVTGPAGVNAGIPPKFNLLKGDPDVDQGGGRLKGLFKSGGLGVFGFLLVRVDGDRVAY
                     DFMVPFTLEVAYSEGNDGVSSRARAVLENRGRFTQLLKGITFLMPVSPAGYSLRATVR
                     NGDRTAVEIGPVPEILEVTPAGPGKNLLRVQATVPPAMAVDLEIEAR"
     gene            complement(14210..>14416)
                     /locus_tag="PLY83_03130"
     CDS             complement(14210..>14416)
                     /locus_tag="PLY83_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29064.1"
                     /translation="PFSPGKRSVLSPSCLSERQIERLCDGDLARQHPELRRSVTLEQV
                     LKSACLLEACFHARFARPAAPDRA"
     assembly_gap    14417..14516
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14517..15070)
                     /locus_tag="PLY83_03135"
     CDS             complement(<14517..15070)
                     /locus_tag="PLY83_03135"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOV29065.1"
                     /translation="MENKREEVVRRLSEWIEKPERLTGGRLPPERDLARELGVSRTLL
                     REAVITLEALGVLEVRERQGIFVRTLQGEDFAISLKTLSLWPEELLVHLMEMRLVMEV
                     PAAGLAAQRRTERELEQMRRCVAHLREVHAAPDGGASSGAAWDSLLHALVVGAAHNPV
                     LTRVYEGLTSTMEKQIATSRRLLL"
     gene            15206..16174
                     /locus_tag="PLY83_03140"
     CDS             15206..16174
                     /locus_tag="PLY83_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOV29066.1"
                     /translation="MKKLLSVFLVALFLVSMAASAFALEIKLAHVVNEKDSFHLAAEK
                     FKALTEKYTNGTVTVTIFPNAKLGDERTLLERMKMGIVDAGIITNGPIINFVPRFGAI
                     DLPFLFRSPEHAYKVLDGPIGNQLFADLEKQGWKGLAWAERGFRNLTNSKRPVNSPDD
                     IKGLKIRVMQNPVYVDSFKALGANAVPMAWTEALTALQQGTIDGQENPLNVIVSFKLN
                     ESQKYMAITRHAYAPAPILMSMVTWKKLNPEQQKAVLKAAQEAAVYERAYNNASEKLW
                     IKELESKGMKVTTPDLKPFLAAVKPVYDTYTPKFGKDFIAAIQNTK"
     gene            16175..16693
                     /locus_tag="PLY83_03145"
     CDS             16175..16693
                     /locus_tag="PLY83_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOV29067.1"
                     /translation="METLNGTTQQEGASRSLLQVLSDSVNRLSERLLFFLMILMIGVT
                     TAQVIFRFFFEALTWSEELSCFLLVLASLLGAAVAFKRGSHIAVTFVTQKLPPSLQKG
                     MAVLVNALGILFFGIVAYYGAVLMKTESSQTTPAMMISMSWIYAMYPVLGAVIILHLA
                     AGMGEILKGGRS"
     gene            16693..17976
                     /locus_tag="PLY83_03150"
     CDS             16693..17976
                     /locus_tag="PLY83_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOV29068.1"
                     /translation="MGWVIFGSFFLLMLIGVPIGLSIGLAALVVFLASGIPLQMVPQT
                     LLEGNNSFALVAVPFFVLAGDILARGGISERIVAFAEATLGRIRGGLSIVSVLASMFI
                     AAISGSGAATTAAVGSALLPELKAKKYDVDFSAALIASAGTIGVVIPPSVPMVLYAVI
                     AGESVAKLFMAGFIPGALMGAGLIAYALWIANRRGYPAGEKLPGAEVGRRFVSALWGL
                     MTPVIILGGIFTGVFTPSEAAAIAVDYTLLVAWLVYRSLDFKKFYELVVNAGVTSALI
                     MFIIATAKLFGWGLAFYQIPEAIAQTLMAFAGNSTFMIYLMINVIILLAGMFMETASA
                     LIILTPIFLPAILGIGGNLIHFGLLITVGLAIGMATPPVAIDIYVASAITGLSMEEVS
                     KPILPMVLILVAVLFLCTYVPSLVLILPRLFMAGV"
     gene            18003..>19351
                     /locus_tag="PLY83_03155"
     CDS             18003..>19351
                     /locus_tag="PLY83_03155"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOV29069.1"
                     /translation="MRSDNAKKGIERAPHRSLMKASGYTDWEIERPWVGVVNAYNAII
                     PGHVHLNRLAAAVKAGVYAAGGLPIEFPVIGVCDGIAMNHAGMKFSLPSRELIMDSIE
                     VMAEGHALDALVMVTNCDKIIPGMAMAAARLNIPTVVLSGGPMMAGLLKGREIDLNTV
                     FETVGKCIAGSATEEDLAEVENAACPGCGSCAGMFTANTMNCMMEALGLALPGNGTIP
                     AVYAERERLAKDAGRAVMALLEKNIRPRDILTMEAFENAVSIDLALGGSTNTALHLPA
                     IAHAAGLELPLELFNTLGEKVPHLCSMSPAGPHHIQDLYRAGGVQAVMSRLSEKGLIK
                     GGCITVTGRSVSENLAKVSVLDDGVIRPLENPYHAQGGLAFIKGSLAPGGAIVKQSAV
                     SEEMLRHTGPARVFDGEEAAFRAIRGQQIRPGDVVVIRYEGPKGGPGMREMLTPTSSL
                     "
BASE COUNT         3726 a         5687 c         6134 g         3704 t
ORIGIN      
        1 agactgcttc atctgcacca gttccagacg ccccttcttc ttcgccatga cgatctctcc
       61 tttcagtctc gatttgagct gcccaaaaac tccttccgat tccaaccctt tcccctctcg
      121 gggaaaagga acatcgctgc gggggcgaga agcgcccctg ttccgagaat ggaaaaggcc
      181 aagccccacc gggccctctc cccaaggaca aaggcttccg taccgagaat ccagccgaaa
      241 aggaggggag agatcacggt ggcaaggtac ccgattgcgg attggaggcc gagcgccgtt
      301 ccccggcagg ccggagaaac ggtttcgctg agccccgcct tgtagacggc agaatcggcg
      361 acggaccaga aaccgatcca ggaaccgacc cccagggtta cgggcagggg ggcgccgtgg
      421 aggaagccaa agagaaattc agccgcgccg ctggccaacc ccgagaggag gatggttttt
      481 tcccgtccga tccggtccga aagcatccca attcctgcta cggcgagcga tccggaaagg
      541 atgatgcagg aggaagacac ccctgagaag gattcggcgc gaggaactgt ccagcccgcc
      601 tggagcgcgc aggcgacgag gaagggaccg acccatcccc agaaggcgta aagttcccac
      661 atgtggccga agtagccaag ggtcaggaga aggggcgctt taatccctcc ggggaaccag
      721 gccggcctgc cccccctgca cgcttttttc gtttcagaag gccttgggga ggcagaaggg
      781 gcgtcggtga caaaaaagca aagaataaag gcggcaagga aaactggcgc cgagatgcag
      841 aggatcccca gtctccagga aaaggcagcg gcgatcggag aggccaccag gtatcccccg
      901 gcataggagg cggtcagcgc cgcggtgtag gccccgatgg ccattcccct cctttgcgga
      961 ggaaaccatg cggccaaaag agccatcccg ggggcgtaga tggctccggc cgaaagaccg
     1021 gtcgccaggc ggagggcgaa gatgcttttg aagccggttg aaaggaaggg gaagaaggtg
     1081 gaaaagacgg ctgttgccag ggttgcccag acgaggacgc gcttgttgcc aaagcggtct
     1141 gcaagaatgc cgctggcgat gaccgttacg acgtagccga gctgaaaggc ggaaaggatg
     1201 gcccctactt gctgcggggt cagcccgaac tcccgaacca ggtgggaaga tacggcagaa
     1261 aaaccgtacc agggcatgta tccgaagaac atggaaacga ccatggcgat gaggatccgt
     1321 gacggctgta ccataaaaaa aagcccgcct tttgttttga taagcaaaac aatgttttga
     1381 aaaaccctgg aaaaaggcta ccatgcctgg ttcctccctg tcaaatcctc cggcttgaca
     1441 gaaagaaacg gagtgtggta gattcctcgc catgatgaat cgatatagcg gtatccggac
     1501 ggaatcgaat cgaatcgcgc ggtggtggtg ggggtcctga cccccgcccc ttcggtttgc
     1561 gctggagtgg gtcgcgggga aaagtgattc ccgcggcccc tttttttatc tggaaacagg
     1621 cgggggccca tacggctccc gctttttcgt ttctaaactt tgcaagggag gatgacgaga
     1681 tgagacaggc gagtacgttg cagaggaacg cgcggggaac ttacggggag tacgggggac
     1741 ggttcgttcc ggattcgatc gtggggctcc ttgaagaact ggagtgcgcc ttcgactcgg
     1801 ccatggccga cccgtctttc gtatcagaga tggaccggct gttaggtgaa tacgtgggga
     1861 gaccctcccc catcaccgaa tgcgccaacc tctccaggga actcaagggg gggcgcctgt
     1921 tcctcaagcg ggaggacctg aaccacacgg gggcgcacaa gatcaacaat gccctgggtc
     1981 aagccctgct ggccaagcgg atgggaaaat cccgtctcat cgcggaaacg ggggccggga
     2041 tgcacggaac ggccagtgcc accgcggcgg ctctcctggg gatgagctgc accgtttaca
     2101 tgggggcggt ggacgtggcg aggcaggccc ggaacgtttc ccggatgaag gcccttggag
     2161 ctacggtggt cgctgtccgg gaaggccagg gaaccctcaa ggaagccgtc gatgcagcac
     2221 tggcagcctg ggtccaggat cctggggcct tttacctgct tggctccgtt gtgggaccgg
     2281 ccccttatcc cgagatcgtc cggaccttcc aatccgttat cgggcgggaa gcccggcgcc
     2341 agttcctcga gcgggtgggg cggctgcccg aagaagtggc agcctgcgtg ggcggcggga
     2401 gcaacgccat gggccttttt tcggggttcc tggaggacac ccaggtgatc ctcaccggtg
     2461 tcgagccggg tgggaagggg ctcgaaaagg ggctcgggtt tcatgcagcg acgctctgtg
     2521 ccggaaaccg gggcgtggtt cacggattcc ggacggtggt cctctcggat gctcttggcg
     2581 agcctgcccc cgtccattcc atcgcggccg ggctggacta tcccggggtg ggacccgaac
     2641 tcgcccacct gcgggacacg gggcgggccc ggtacgtcgc ggtaaccgac gaggaggccc
     2701 tcgatgcctt cagaaggctt tgccgctccg aggggatcat cccggcgctc gagagcgctc
     2761 acgccgtggc ctacgcgctg aagcggctcc cggagatgga accgcaccag tccctgctgg
     2821 tcaacctttc gggccgcggg gacaaggacc tcgacacggt gtttcccgag aaggaaacaa
     2881 atcacttccc ggttggggcc ggaggcctta acgggatcca gtcgaggggg ttgttttacg
     2941 tctaggagcg gcttcctcgg ctttttgggc gataagggcg gccccgatgg ccccggcgta
     3001 gggggcgtcc aaagaagtga caacgggccg tcccaggcgc ttctccagga gccgcaccag
     3061 gttgcggctc ctggagaccc ccccggtgaa agcgacgggg ccggatcctc ccagcttctg
     3121 aagcatcgcg caggcacggc ccgcgatgga atcgagaagt cccagggcca gcgagtgctt
     3181 gtctacccct cgggcgagga gactgaccac ctcggattct gcgaagaccg tgcacatgct
     3241 ggaaatgggc tgagggggca catcctccgg caggcggctg aaatcgtcca gttccatgtc
     3301 cagcaaggtg gccatgacct ggagaaacct tcctgttccc gccgcgcatt tgtcgttcat
     3361 caaaaaatcg cagacggttc cccccctgcc gagggagatg gccttgctgt cctgcccgcc
     3421 aatatccagg acggtttccg ctccggggaa aagaaacgcg gagcccgctg catggcacgt
     3481 gatctcggtt actgtaggtc cggctttccc gaggctgttc ctgccgtacc cggtgagaac
     3541 gagaggcgga gaatcggacg gatcgatccc gttcctggcg aaaatttccc ggagggcctg
     3601 ttcgccagtt tctttggggt tccagcccgt gggcaccatg atgcggtccc ggaccgagcc
     3661 gtcgaagagg acggccttga tgcccatgga gccaagatca atgccgatgc tcaatctcat
     3721 ggtcgctgaa ccaccagttc gatggggatg ccgagccttc cggcttcttc ccgaacggca
     3781 ccggccgcaa tcgcgtcgaa ccgcagcgag atgccgcagc tgtcggccag gtggcggggc
     3841 gtgggacaga tgtcgtcctt caccccgagc tccctcaggc ggcggtgaag gatcattccg
     3901 tccgtcgcgg ttttgaaaag cgcgtacgcg aaaaccaagg atagtttccc cttcctcttt
     3961 ctcccgggag cgggacctgc ctgccttcgg cctgctctag tccctgagac tttctacaaa
     4021 agcctcgatc cgggtgcgca gctgcccctc gtcacctgag gcgtagtcgg tctcgagggc
     4081 caaaaacggg atgccttcgg actgcaagcg tttccggact ttttcgtttt ctacctggta
     4141 ggtcgtgcag aagctcaggt taagccccac cactccatct gcgcggtatt cgcgggctag
     4201 cctcacgaca tcgtcggcgc gcccttcgtt cggggtgaag caggcgcagt ggatccggtc
     4261 gaaatagcgg tgggtgatcg cgtcgaccag ttcttcgacc gtcgatgcct tcgacatatc
     4321 cacgcggctt tcgaaatatc tcgttccggt gcagttctct tcgaccacca cagcggcacc
     4381 gctggtttcg atgattttgt gaaccttcca gttgggaacc acgatcgggg taccggtgac
     4441 gaggagacgc ttcgttccct ccgggaagac ggtcttgccg gatttgattc tttcttcgca
     4501 ttcctccgcc agggcggcag cctgcgagat gaatcggctg gcatcgtcat aaaaagccac
     4561 ttggctggcc aggagcatgt ccagcccgct gatggggact ttcggggcct tccgggtttc
     4621 atacaggcgc tgaaggacgg cacgcttccg atccaccgtc tcgatggcag aaagcagccc
     4681 gtcgaaagtg aggcggttgc cggtgacttc ttccagtttt gccacgagat cggccatgga
     4741 tttgcggaag agggcaaacc cggcggcagt cttggtgttg ggcaggtcca tgacgtaggt
     4801 gggagcgatg tcacccagga cctcccaggc cttcttcttg ccgtcgcagg tgttttctcc
     4861 cacgaccagg tccaccgact ggaagtaggg gcaggtccgg gaaaccttgg ccccgatgcc
     4921 tgccttgatc agggggcaga ggttccgggg aaggacctgt tcccccgctg gcacccagaa
     4981 ctcggagccg gcacacaggc cgaccatgat cccgtcgagg gccaggacga tctcctcggg
     5041 aacgtagatg cagaagctcc cgatgatttt acccccgcgg gccttgtggt ccaggagttc
     5101 ctggatcctt aggccgtgaa cctcggcgat caccatgttg aaataatcca tcccctgggg
     5161 acggtccttc tgggtcagga aaacctgctc gaatgcgggc ggaagtgccg cgcaaagctg
     5221 gtcgtgagtg gccaggtcca tgccaagccc ttcccacatc ttgtgataat cggccatgag
     5281 aactcctcct tgaatgtgga cagacaggaa aaaacgcact tcaggcgttc tgcccgaagc
     5341 ctcgggttgg gaaaagactg tctttttgcc ggaggagagc cctgcggaga ggagcgcgtc
     5401 agaaggaacc cgggaaagga gctgggaggg ccgatccttt ggcggcgtcc ccgctgcccg
     5461 gggcaatctc cgaccgcgat gcagaccctg catcgcccgg tttgcgtgtt caacgccgcg
     5521 ccttctggat catcctcacc cccccttttc ttctccttcc gctttgccgg aacccatcat
     5581 aagggtttca accatggcgg gcaagcgtca agcggtggcc ttccagtgtt tgaagccctt
     5641 gccaaagacc tccgtggcgt cggtctggat caggaaggca tttggatcgt gctccgccag
     5701 gaaaagcttc aggtcggcca cctggcgcgg aagcagcagg gacatcacga tcggccgttc
     5761 ctttccggtg taggcccctt cgctgtggat cacggaagcc ccgcggcgga gttcttccag
     5821 gatgaatcgc cggatgtcca ggtgcttttc ggaaatgata aagacctgga cgcaccggtc
     5881 gaaggaccgc aaggccccgt ccaggaaagt cgagctggcg aacagcatgg ccacggcgta
     5941 gagcgtgttt tcgatcccga cgatgaagat cgcgggaacc aggacgaaag agttgatcca
     6001 gaaagtgaac tttccgatct ccatcccggt tttcctgcgg acgatggcgg cgaggatgtc
     6061 cgttccgccc atggaccctc ccgcgttgaa gacgagggcc ccgctgatcc cgttgatcgc
     6121 cgccgcggta aggaccgcca gcagcttgtc gctgagatgc agggagggga acatcctgaa
     6181 gaggtccagc gaacccgaga agaggaggac cgagtaagcg gtgaggtaga ggaagcgttt
     6241 cgggagaacc ctccagccca gggcgaagag gccgatgttg aagatcagcg tcatgagccc
     6301 gacgggaagt cccagcgagt agttcagcag gatggaaatt cccgtgaagc ccgtgctggg
     6361 gaagcggtag ggaagcacga acagggaaat ggaaagggaa tacaggaccg tccccaccgt
     6421 aacacgcaaa aaaccggggg cttccctttt cagggtcctc cttgtccgga gggtcaagcg
     6481 ggaaagaaag cgcatggtct acctcctccg aaaagaatca gggataccgt acccgtagtg
     6541 taaggcaaag ttgccctttt gttccgcaga gaaccggctc ctgtcgtgac caatcttgac
     6601 taatcgggta aaatacatct ccggaaaaaa tcgaaattga aaaacgggag gtcgatttcc
     6661 cttgagtgaa actcgaagca aagaacacct ggatttccag gcggaggcga aacaggtcct
     6721 ggacctgatg atccatgccg tttactccaa caaggacgtg tttctccggg aacttgtcag
     6781 caacgcgtcc gacgcgctgg acaagctgag gctggaatcc ctgacgaacg aagcactccg
     6841 ggacgcggtc cagtctcccg cgatcaacct cgaacgggac gcggaaaaac ggacgctttc
     6901 ggtctctgat aacgggatcg gcatgacgaa ggatgaaatg atccggtacc tcggaacgat
     6961 cgccaagtcc gggacgaaag agtttttgcg cctcctgaag gaagccgggc ggggggggga
     7021 ccccgaggcg ctgatcggac agttcggggt gggattctac tcctccttca tggtggcgga
     7081 ccgggtgacg gtgatcagcc ggaaagccgg gaccgacgag gcctggaagt gggaatcgga
     7141 aggggacgga gggttcaccc ttgaaccggc cgagcgggac agtttcggga ccacggtgac
     7201 cctgcacctc cgtccggccg atgaagagga cggcctgagg gattacacgg acgagtgggt
     7261 cctgaaggag atcgtccgga aatattccga tttcgtggct tacccgatcc ggttgggtgg
     7321 cgaaaccctc aacagcatga aggcgatctg ggcccggccg aaatcggagg tcaaggatac
     7381 ggattacgac gagttctacc ggcatatcag ccacgactgg aatcctcccc tcaagcggat
     7441 cgcctggaag gcggaaggaa ccgtcacctt cgatgcgctt ctgttcatcc cttcgaaggc
     7501 cccgcttgac ctgttcatcc gcgacgtcga ccgggggctc cagctgtacg tgaagcgagt
     7561 gttcatcaag ggagactgcc gggagctttt gcccgactac ctgcgtttca tccggggagt
     7621 ggtggacagc gaggatctgt cgctcaacat gtcgcgggag atcctccagc agaaccgtca
     7681 gatggagctg atccagacgg ggctgacccg gagggtcctc tccgccctgg acgagatgcg
     7741 gacaacggac ccgcagagct accgcacgtt ctggaaggaa ttcggccggg tcctgaaaga
     7801 aggacttttc ctcgacggca aaaaccggga aaaaatcctg gagctttgcc tcttcaaatc
     7861 ttccatttcg ggagacgagt gggtcagcct ggaagaggcg gcgtcccggg tcaccggtga
     7921 ccggaaggag atcttctacc tcctcgggga ggaccggtcc gtgctggaaa aatcccccgt
     7981 tctggaagcc ttccgctcca gggggatcga agtgctgttc atgaccgacc cggtcgatga
     8041 gatctggctg caggcagtgg acgggttccg cgattaccgg ttgagggatg ccgccagggg
     8101 ggatctcgat ctcgaaagcc cttcggaaaa agagacggga cggaaagagc gggatgaagc
     8161 ggaaaaacgg ctgcgcccgc tgatggatta cctgaaaaac cggcttgcct cgtcgatccg
     8221 ggaggtccgt ctctcggggc gcctcacgga ctcgccggcc tgcctcgtgc aggacgagcg
     8281 ggacccttcc ccgcagtttg cgaaggtcct tgcttccctg ggcagggaag cccctcaaac
     8341 ccagcggatc ctggagatca acccggcgca tcccatcatc gccggaatgg caaaaatgct
     8401 ggaggaagcc ccggcaagcc cgacccttga ggcgtacgcg gaactcttgt acggaatcgc
     8461 ggccctggcg gaaggcagct tgccgcagga tgcgccgggt ttctcccgaa agctggcgga
     8521 ctttcttcgg gagaaggtgg attcagggaa cgattcccga aaagacgtct tgccagaata
     8581 aagggttaag cttatatttg aatgaaggaa agggatccgg aagattctgg ccgcatgatc
     8641 aaaaagggga ggtgtctagg gcgtgctgac gaaccgtcac gtggaatgcc cgcactgcgg
     8701 gaccgagttc aatgtctacc tgaactacaa ggaaggcaag ctggtctgcc ccggctgttc
     8761 caagaatttc gtgccggaat atttcaacct ggactacaag cagctcgaag aagaggctga
     8821 gaagaaataa ggcgggaaaa aagaggaaac agaaaggtcc gggggaaagg gaggcctccc
     8881 tttcccccgg acctttctcc tacaggtagt accgcaagta gacgtacgcc gtcgaagcgg
     8941 ccaacgtcag aagggtgatg ggaattccct ttcggagaaa gttcccgaag gtgagggggc
     9001 ggcccgcctt ttccgccagg cctgccatca cgaggttcgc ggaagccccc accaaggtcg
     9061 catttccacc gaggcaggcc ccaagggaca gggcccacca gaggggtcgg gcatcgagat
     9121 gggcaaggga ggccatgtcc ttgacgaggg ggatgaccgc ggccgcgtag gggacgttgt
     9181 ccacgacggc cgagatcgtc ccggaacccc agacgaggaa catcgccagg attttcggat
     9241 ggtcgccggc ggcattcgcc aggaaacggg cggcgaggcc gatgatgtcc aggtgttcca
     9301 gggttccgac aagcatgaag agggtcgaga agaagaggat ggtgacccag tcgatctcct
     9361 cgagcgcggt ttccaccttc acggggcaga cgaccagccc gagggtcgct cccgccaggg
     9421 cgatggtggc cgcttccagg tgcagccgac cgtgaaggaa gaaggcggca agaaccaggg
     9481 ccagaaccgt caggatcctt gccgcgagag ggcggttgag gtgtttccgg agggtgtgga
     9541 acccctcgat caggtcttcg atgggacggt tctgcgagag atcgcgagcg taaaagacgt
     9601 aaagtccaag atacatggcc agcagggcga gagccgccgc gggaccgacg ttcagcagga
     9661 agtcgttaaa ggagagtccc gccgcggagc cgatcaggat gttgggagga tccccgatca
     9721 gggttgccgt tccgccgatg ttcgaggcga agacttcggc aaagatgaac ggcatggggt
     9781 ccaggtccag ggtttcgcag acggccaggg cgacgggccc gatcagcagg atcgtggtaa
     9841 cgttgtccag gaatgcggaa acgaccgccg tcaggaggga gagggacacg agaaggcgcc
     9901 acgggttgcc tcggctctgg cggatggcga tcagggcggt atactggatc aggccggtct
     9961 gcttgacgat ccccacgatg atcatcatgc cgatcagaag gccgatggtg ttgaaatcga
    10021 tgaagctgaa ggcatcgtgc tgttccacca gccgcaggag gaggatgccg gagagcccaa
    10081 ccagcgctgc tttcaggcgg tgcatgcgtt ccgtgacgat gagggaatag gtgaaaatga
    10141 aaaggccgag ggcgatccac tgtgttgccg acattttccc gtctcccttt cggggatttc
    10201 tcatccttcc agatgacggc gtattgtatc agcaaccgag gaaacccgca atgggatttt
    10261 gaaaagaccg gtattttcag aaaaaacaga aaaacctgtc gatgcttggg ccaatcaagg
    10321 ggggaaaccg atggggttca ggagttttct gccgaaatgt tcgccgtgaa tgctgctttg
    10381 gggagccgtg gcgctcgccg tcatcctgct gtggattctc aaggccctga ggggttaggg
    10441 agaaatgggg ggccggaatg cgtccggccc cccgagggtt atttcctgaa gagatagggg
    10501 tagcgttttg ccttgctgct ctccccggct ttcggctctg ccgttttcgg tggagcggga
    10561 gcgcggaaac ctgccagcga aagggcatcc tcggaagaga acgcttcctc ggaggctttg
    10621 ccgatcaggg caaagatgcg gcctgtagcc agctggatgt tttcccgtat cgattccttg
    10681 atggtttcgg agagagcccg gatttgccgg tccaggtcgt cggttttctg gagcgccccg
    10741 ccgacctgct ggtaaaattc gttaagccgg ttttcgaaat ggttccggag gtcccgctcc
    10801 agggccgcga actgggccac caggtcggaa gaaagcccct ggatgtcttt ccgttcccga
    10861 agaaccattt cttcaacggc agacagcttg atggtctgtt cgcggtagag cttttccagg
    10921 gcaagaagcc gttctgcgac gtctggaggg aggtcccggg taagcgaggc aagccggctt
    10981 acttccgagg cgaggggcct gacggtctcg agggcctgtt ctccctgttc ggcaagcctg
    11041 cgggattttt cctcgagccc gttcaacagt tcctttcggg tcgattccac caggttgccg
    11101 aagaaggtca ggcgcttttc ctggtcgtcc ttgcggcttt ccgcctcctg gaggatccgc
    11161 ttgcgcgcct cctccatcgt gtcggtaact tcgtcctgga tgcgggtcat gcgttcgttg
    11221 agcgacatga cggaatcacg caacccccgg gtttccaggg aaaaccgatc gtgggtctcg
    11281 gtgatgcgcc gatccaggtt ttcctgcaga cgcgacgagg tttcccggat ttcccttgcg
    11341 gcttcagaga tccggctatg gcagaacaaa agcccgaccg caccgattcc gaacaggatc
    11401 agcagggtta gcgtgttgaa ttcaaaattc atccgatgcg cctcctcaat tcgaggattc
    11461 gattgagaaa tgattttttt agtaacgctc ctatcataac atgcaagaac gttttgggga
    11521 ccctgtcaag cgggacaggt ggattggagg attcgccatt gaaccggtat cctggaaaaa
    11581 tcctgctgca cgttttgttt ggattccttg tcacggccta ttttctcagc ttctttttcc
    11641 gcgtttccgc ggcggtcgtg ctgccgcgcc tggcttccga aatcggcatg acggccgcca
    11701 tgacgggttt catttccagc ctctactact atgcttatgc cttcgtgcag cctctctgcg
    11761 ggtccctgaa cgaccgctgg gggccgcttc gggtggttgg aacggggctt tctgttgccg
    11821 ctgccgggtc tgccatcttc atgttcgctt cgactccggc gacccttgcg gcggggcgcc
    11881 ttttgaccgg tttgggcctc gccccgatgt tttccggggc tctcgtctac cagtccaccg
    11941 cctttcctcc ccggcgctac gccttttact cgtccgtcac gatcgcggtg ggaaacctgg
    12001 gggcggtcgt ttcggtcggc ccgctcggct cggcgatcga ccggtggggg cggggcccgg
    12061 ttttcctttt cctgtcggcc ctgagccttt tcatggcagg tcttctcttc ctgaacgcga
    12121 agtccgatcc ggtgagaggt tcgaaccagg gggggcgggg acccaaaacg cccttttcgg
    12181 cccacctgaa gacggggttt tccaccctgt ggacttcaag ggagtgccgg tgcctgacgc
    12241 ttctttgggg aatttcggtc gcggccatgc tggccttcca ggggctctgg tccgtcgcct
    12301 ggtaccagac cgcctacggg gttgaggccg ggattgcccg gaactgggcc ggtgccgtgg
    12361 gcatcgggat catgaccggg acgcttttgg gcgggcgctt ttcttccggc cccggcgggc
    12421 ggatgaaggc catcggatgg gggttgtcca tgaacctcgg agcctggatc gcgctctggc
    12481 tcgggatcgc cctgcgcctt cccctgaccg tgacggggag tctcggtttt cttctcgggg
    12541 gagccaccgg attcctggtt gtccatttcg cctcggccct gaacgagatg accccccgcg
    12601 agcgggcggg ggccgtcctg ggggtggtga acatgctcgt gatggtgagc gtgatcgctt
    12661 tccagtgggg aaccggcgcg atcctggccc gcttcccggg agcggcggcc ggttcttcct
    12721 ctcccagggg gtttttggtg acgttcggga tcgtgatcgc cctggtggcg gcgagctccc
    12781 tggccttcag ggggatgcgg cgcttccccg aagcggtgag gaggggttcg gaatgaaaag
    12841 aagaacggtt tttcttctcg ccctggcctt cgcgtgcttt tccctcgtgc gggccgatgc
    12901 gggcgtgatc cgctcggaaa aggcggatga gtggggagga atggccggcc tggggaaaaa
    12961 aactttccgc tttgtggtgc ttggagacac ccagcccctc ggtccgggac ggagtctttc
    13021 ccctctcgtc gagcggcttt tgaaggaaat ctcctggttg ggaccggact tcgtggttca
    13081 cctgggagac tcgatctccg gctacgggga ttccctggaa gaggcccgga ccgaattttc
    13141 ggccctgaag gagaaaatcg accggctgct gcctgccgtc ccgttttttg ccgtgcccgg
    13201 caaccatgac caggcaactc caggagcgct ttctgcctac cgggaaacct tcggctccct
    13261 gctctactac gattttccct acggcaacgc ccgcttcgtg atcctcaacg gcaactttcc
    13321 cgcctggatg gctcccaaag ggaaagacta cgggtttttc aacctgaacg acggcatgca
    13381 tgtcctgccc atggtcgact gggcaaacga agccttcagc cggggaggga agatgctgtt
    13441 cgttttcctt cacacgcctc cctttcccgc ctacacccct cctccgggga aggggaccca
    13501 cggtttcctc accgagtcaa accgggatgc tttcgtggcc ttgcttccca agggccggac
    13561 caaagcggtt ttttccggcc acgagcatct gttctacgta gaggagcgca acggaatcaa
    13621 gttcttcacc ctgggaaccg gaggcggccc cctttccggc cccgtcacgg gaccggcggg
    13681 ggtgaacgcg ggaattcccc ccaagttcaa cctgctgaag ggggatcccg atgtcgacca
    13741 gggcgggggg cgcctgaaag ggcttttcaa gtccgggggc ctgggcgttt tcgggttcct
    13801 gctggtccgg gtggacgggg accgggtcgc gtatgatttc atggttccct tcaccctgga
    13861 ggtggcgtac tccgagggca acgacggggt ttcgtcacgg gcgcgggccg tcctggaaaa
    13921 ccggggccgg ttcacccagc ttctcaaggg aatcacgttc ttgatgccgg tctcccccgc
    13981 cggctacagc ctgagagcca ccgtccgaaa cggggatcgg accgcggttg aaatcggtcc
    14041 ggtcccggaa atcctggaag tcaccccagc cggacccgga aaaaacctcc ttcgcgtcca
    14101 ggcgacggtt ccgccggcca tggcggtcga cctcgagatc gaggccaggt aaacacggag
    14161 gcccccggag gggcctccgc tctacgagcc aggctttcgc gggcggggct tatgcccggt
    14221 caggggctgc cggccgcgcg aagcgggcat gaaaacaggc ctccaagaga caggcgcttt
    14281 tcaggacctg ctccagggtg acgctcctgc ggagttcggg atgctgccgc gccaggtcgc
    14341 cgtcgcacag gcgctcgatc tggcgctctg agagacaaga cggggaaaga acgcttcgct
    14401 tgccggggga aaaaggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntgga
    14521 gcaacagcct gcggctggtg gcgatctgct tttccatcgt tgaggtcaac ccttcgtaaa
    14581 cacgcgtgag gacggggtta tgggcggctc cgaccaccag cgcgtgcagg agcgaatccc
    14641 atgcggcccc ggacgaagcc cccccgtcgg gagccgcatg gacttccctg aggtgggcaa
    14701 cgcaccggcg catctgttcg agctctcgtt ccgtgcgcct ttgggcggca aggccggccg
    14761 cgggaacttc catcacgagc ctcatttcca tcaggtgaac cagcagctct tcgggccaaa
    14821 ggctgagggt tttcaggctg attgcaaaat cctctccctg cagggttcga acaaagatgc
    14881 cctggcgctc ccgcacttcc agcaccccca gggcctccag ggtgatcacc gcctccctca
    14941 ggagcgttcg gctgaccccc agttccctgg cgaggtcccg ttccggagga agccgacccc
    15001 cggtcaaccg ttccggtttt tcgatccact ccgatagcct gcgtacgact tcctcccgct
    15061 tgttttccat gtcggcccgc ttgcccccct ctttcacaga aggaaattcc tgtgcctctt
    15121 ttcagttcga taggccaatg ataaactata gggcagattt ctggtaggtc cttggttgga
    15181 ccaaaattca aggaggtgcg cagcgatgaa aaaactgctt tccgttttcc tggtggcgct
    15241 gtttctggtc tccatggccg cgtccgcgtt tgccctggag atcaagctgg cccacgtcgt
    15301 gaacgagaag gactcgttcc acctggcggc cgagaagttc aaggccctca ccgagaagta
    15361 cacgaacggc acggtgacgg tcacgatctt ccccaacgcc aagctcggcg acgaaagaac
    15421 cttgctcgag cgcatgaaga tgggcatcgt cgacgcgggg atcatcacga acggccccat
    15481 catcaacttt gtcccccggt ttggggccat cgacctgccc ttcctgttcc ggagcccgga
    15541 gcacgcctac aaggtccttg acgggccgat cggcaaccaa ctgttcgcag acctggagaa
    15601 gcagggatgg aagggcctgg cctgggcgga gcggggattc cgcaacctga cgaacagcaa
    15661 gaggccggtc aattcccccg acgacatcaa gggcctgaag atccgtgtca tgcagaaccc
    15721 ggtctatgtc gactccttca aggccctggg ggccaacgcg gtgcccatgg cctggaccga
    15781 agccctgacg gccctccagc agggaaccat cgacgggcag gagaaccccc tgaacgtcat
    15841 cgtctccttc aagctcaacg agagccagaa atacatggcc atcacccgtc atgcctatgc
    15901 tcctgccccg atcctcatga gcatggtcac ttggaaaaag ctcaatcccg aacagcagaa
    15961 ggccgtcctg aaggcagccc aggaagccgc ggtctacgag cgggcttata acaacgccag
    16021 cgaaaagctt tggatcaagg aactggagag caaggggatg aaggtcacca cgcccgacct
    16081 gaagccgttc ctcgcggccg tgaagccggt ttacgacacc tataccccga agttcgggaa
    16141 ggacttcatc gcggcgatcc agaacaccaa gtaggtggaa acgttgaacg ggacaacgca
    16201 acaggaggga gcttcccgct ccctcctgca agttctcagc gattcggtga accgccttag
    16261 cgaacgcctt ctgttcttcc tgatgatcct gatgatcggc gtgacgacgg cccaggtgat
    16321 cttccgcttc tttttcgagg ccctcacctg gtcggaggaa ctctcctgct tcctgctcgt
    16381 gctggcttcc ctcctcgggg ctgccgtggc cttcaagcgg ggcagccaca tcgccgtgac
    16441 tttcgtgacg cagaagctcc cgccgagcct gcagaaaggg atggccgtat tggtcaacgc
    16501 tcttggcatc ctctttttcg ggattgttgc ctattacggc gcggttctca tgaaaactga
    16561 atctagccag acgacgcccg ccatgatgat ctccatgtcc tggatctatg cgatgtaccc
    16621 ggtcctgggg gcggtcatca tccttcacct cgccgcaggc atgggcgaga tcctgaaggg
    16681 aggccgttcc tgatgggctg ggtcatcttt ggctccttct tcctgttgat gctcatcggg
    16741 gttcccatcg gcctttccat cgggctggcg gccctcgtcg tcttcctggc gagcgggatt
    16801 cccctgcaaa tggttcccca gacgctattg gaaggcaaca actcttttgc cctcgtggcg
    16861 gtgccgttct tcgtcctggc cggggacatc ctggccaggg gcgggatttc cgagcgcatc
    16921 gtggccttcg cggaggcgac cctgggccgg atccggggcg ggctttcgat cgtttcggtg
    16981 ctggcctcga tgttcatcgc ggcgatctcc ggctccggcg cggccacgac ggctgccgtg
    17041 ggatcggcgc ttttacccga actcaaagcg aaaaaatacg acgtcgactt ttctgccgcc
    17101 ctcatcgctt cggcgggcac catcggcgtg gtcatccccc ccagcgtccc catggtcctt
    17161 tacgcggtca tcgccgggga gtccgtcgcc aagctcttca tggctgggtt catccccggc
    17221 gccctcatgg gagccgggct catcgcctat gccctctgga tcgccaaccg gaggggctac
    17281 ccggcgggag agaagcttcc tggggcggag gtggggagac gtttcgtctc ggccctctgg
    17341 gggctgatga caccggtcat catcctggga ggcatcttca cgggcgtgtt cacgccgtcg
    17401 gaggccgcgg ccatcgcggt ggattacacc ctcctggtcg cctggctggt ctacaggtcg
    17461 ctggacttca agaagttcta cgaactcgtg gtcaacgcgg gggtcacgtc cgccctgatc
    17521 atgttcatca tcgccacggc gaagctgttc ggctgggggc tggcgttcta ccagatcccg
    17581 gaggccatcg ctcagaccct catggcgttc gcgggcaaca gcacgttcat gatctacctg
    17641 atgatcaacg tcatcatctt gctggcgggg atgttcatgg aaacggcgtc ggccctgatc
    17701 atcctgaccc cgatcttcct tccggccatc ctcgggatcg ggggaaacct catccacttc
    17761 gggctcctga tcaccgtcgg cctcgcgatc ggaatggcga cccctccggt ggcgatcgac
    17821 atctacgtgg ccagcgcgat caccgggctt tccatggaag aggtcagcaa gccgatcctg
    17881 ccgatggtcc tgatcctggt cgcggtcctg tttctttgca cctatgtccc ttcccttgtc
    17941 ctgatcctcc cccgcctgtt catggcagga gtctgaccct gagaaaggaa gtgtgagaaa
    18001 gaatgagaag cgacaatgcc aagaaaggga tcgagcgggc tccgcaccgc tccctcatga
    18061 aggcttccgg ctacaccgac tgggagatcg aacgcccgtg ggtcggcgtc gtgaacgcct
    18121 acaacgcgat catccccggg cacgtgcacc tgaaccgact ggccgccgcc gtcaaggcag
    18181 gggtttatgc cgcgggcgga ctgcccatcg agttcccggt tatcggggtt tgcgacggca
    18241 tcgccatgaa ccacgcggga atgaagttct cgctccccag ccgggagctg atcatggact
    18301 ccatcgaggt catggcggag gggcatgccc tggacgccct cgtcatggtg acgaactgcg
    18361 acaagatcat cccgggcatg gccatggcgg ccgcccggct gaacatcccc accgtggtcc
    18421 tgagcggggg tcccatgatg gcgggactcc tgaagggacg ggagatcgat ctcaacacgg
    18481 tcttcgagac ggtgggcaaa tgcatcgccg gtagcgcgac cgaggaagat ctggccgaag
    18541 tcgagaacgc ggcctgcccc ggctgcggtt cctgcgcggg gatgttcacg gccaacacga
    18601 tgaactgcat gatggaggcc cttggcctcg cgctgccggg caacggcacg atcccggccg
    18661 tctatgccga gcgggagcgc ctggccaagg atgcgggccg ggccgtcatg gccctcctcg
    18721 agaagaatat ccggccgagg gatatcctca cgatggaagc tttcgaaaac gcggtttcga
    18781 tcgaccttgc cctggggggg tcgacgaaca cggccctcca cctgccggcc attgcccatg
    18841 cggccgggct ggaactgccc ctcgagctgt tcaacaccct cggggaaaag gtcccccacc
    18901 tgtgtagcat gagcccggcg ggaccgcacc acatccagga cctttaccgt gccggagggg
    18961 tgcaggccgt gatgtcccgg ctttcggaaa aaggactcat caagggcggc tgcatcaccg
    19021 tgacgggaag aagtgtctcc gagaacctcg cgaaggtgtc cgtgcttgac gacggcgtga
    19081 tccggccgct cgagaatccc taccatgcgc agggagggct cgccttcatc aaggggtctc
    19141 tcgctcccgg cggagccatc gtcaagcagt ccgccgtatc cgaggagatg ctgcgccaca
    19201 cgggccctgc gcgggttttc gacggagagg aagcggcgtt cagggcgatc cgcggacagc
    19261 aaatcaggcc tggcgacgtg gtcgtgatcc ggtatgaggg gcccaagggg ggcccgggga
    19321 tgcgggaaat gctcaccccc acgtcctccc t
//
LOCUS       DAOOVN010000018        19215 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_1947_length_19215_cov_14.634760, whole genome shotgun
            sequence.
ACCESSION   DAOOVN010000018 DAOOVN010000000
VERSION     DAOOVN010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 19215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19215
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1947_length_19215_cov_14.634760"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PLY83_03160"
     CDS             <1..345
                     /locus_tag="PLY83_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HOV29070.1"
                     /translation="LPLWGICLGLQVMALAAGARTYKLPYGHRGVNHAVGEAEGPRGW
                     ITSQNHGYAVDAESLSGTGFTVSMVHLGDGSVEGIVSERLRAWSVQFHPEAFAGPKDT
                     GFLFDEFLKGLN"
     gene            346..3516
                     /gene="carB"
                     /locus_tag="PLY83_03165"
     CDS             346..3516
                     /gene="carB"
                     /locus_tag="PLY83_03165"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOV29071.1"
                     /translation="MKSSMEKSIQTVLVLGSGPIRIGQAAEFDYSGSQACRALREEGV
                     KVILLNSNPATIQTDPGLAEVTYIRPLTPEVVEDILKAHRPDGVVATLGGQTALNLLV
                     ELNRQGVWERYGTLPLGTSPASVERAEGREPFRDLMRSIGEPVVESRIVTEPQAALEF
                     AAHVPFPLVIRPDFTLGGTGGGVAFDEGSLVERVQEGVAASPAGRVLVERYLSGWYEV
                     EMEVVRDGFGNALCVCGMENVDPMGIHTGDSIVVAPVLTLSDSQWQNLRRAGLKIVNA
                     LDIRGACNVQFAVSPGMEEYAVIEVNPRASRSSALASKATGYPIARMAAKIALGKSLA
                     KMPNPVTGVGSALSEPAIDYVAVKFPRWPFDKFPDADPTLGTRMKSTGEVLAIGPNFP
                     QALLKAMRSVEWGGGLRPAGCAQLSERALWAEVRRPTHRRLWFILELLRRKEDVESLA
                     QASGIAPFFLRQMAKVVAAERNLEKRGIQDPEALAEAKWLGLSDRDIAALLETKEEAV
                     REVRLSQGIGQGFREVDGCAGETPAGSRYLYGAYGVSSDGTPEGNTPAVVVLGSGPIR
                     IGQGVEFDYCCVKAVEALKSLGIRAVMANNNPETVSTDHDLSDALYLEPLTVEDVLPI
                     IRRESARGVFASFGGQTALKLGMELAEAGVPLLGTGLGAIEAAEDRGQFSTILKDLGI
                     SQPEGGAVRSVEEALKLADRLGYPLMVRPSFVIGGLAMALVESESDLRSVLSQAFEAM
                     PGQSVLVDRFVSGTEYELDAVCDGADIVVPGIFEHLDPAGIHSGDSMAVFPDLSLNES
                     RRQEMVKIASSIAEALDVRGLLNVQFVENGGRLTVIEANPRASRTVPIAGKLTGVPLV
                     ELAVRVALGQRLRDLGVPLGLLPPRGPLGVKVPVFSTEKLPGVDSRPGPQMQSTGESL
                     GVGECVATALWEGLGGAGWVLPEKGRILVSVNDASKAGACSVAATLFGLGWEIEATEG
                     TAGYFRRWGLPVTTAEKGQALVNALRGRRWDLVVNVAGSRLGALKDGYAIRRAAIETG
                     IPCVSSLPVASALALAMAVRHGNMKP"
     gene            3547..5226
                     /locus_tag="PLY83_03170"
     CDS             3547..5226
                     /locus_tag="PLY83_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOV29072.1"
                     /translation="MGGFKRWLAGALGALVMAVPLALPAQGAGVDLERIRGPKVRDLY
                     LVVVRDPDAAILALERGKLDILGDVTRPSDIRRLSKDPRFSLSMAENFHMAQVGFNLR
                     KFPWNVQELRRAAAMAIPRDRLVRELFSGYSAPLTTFLPPVSPYCDKSAAAPGLDVEG
                     ARALLKKAGWTWNASGILVPPGSKTPLPPATILSPTAGVAPTTAELADRIAAALREIG
                     IPVKAEPLDFAAMVQRIDERKFDLYLMAWQLGRDPDSLYSFYHSSMDVRGGYNQTGLK
                     DAVMDGLLESLRYSPDEPAARKAAREVQKALADRLPIIPIYTRYSVAAVSREWKGAVA
                     TKASAADNSWSLLSMEPASGREKPLYWALGDEPRTLNPFTASTATDWQVLGLLYDTLL
                     AVSPNDLSDLPWLARSWKVETVAKAGAPKTRLTFSLRGDVVWQDGKPFTAKDVKATLL
                     FLKKHAIPRYFDSVRDIESVTVPDDRTVVVTMKNVSYWHLHSIGGLPLFPAALLDRIR
                     DWRSWQPSRTPLPGKTGLTELYGTGPFVFRESKAGDFVRFSRNERFWLIPR"
     gene            5232..6209
                     /locus_tag="PLY83_03175"
     CDS             5232..6209
                     /locus_tag="PLY83_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOV29073.1"
                     /translation="MKRYWTRRLGGAACVVAIVLVANFFLFRLMPGDPVATVLDPRFS
                     PEAKAQLEALYGLDQPLGVQFLLYLRRMATFDFGLSFSTQRPVLDEILERLPNTLALL
                     VPAYLLSVLLGTALGVIAARRRGQLAEKAVLAAGAVSFSFPSFFVQMVLLLLFAYVFP
                     VFPLRGSLSIPPPEGTWRILADRIWHLALPVFSLVLLGFGGWALTVRNLVVKVMGEDF
                     MILARAKGIGERRLLWGHALRAALPPLLTLFLLSLPGVVSGAVLTETVFSLHGVGRFL
                     LDSVLGQDYPAAGGAFYLLTLITVGSNLLADFLVQLADPRIKTGGEESR"
     assembly_gap    6263..6362
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6363..7227
                     /locus_tag="PLY83_03180"
     CDS             <6363..7227
                     /locus_tag="PLY83_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOV29074.1"
                     /translation="RWTLDLDARDMAFRISLGLSPFDESLRGLFDKHGTIRVRIESDV
                     PVERSSLMVQIPGERWGLFGTDQRGRDVFLLFLYGIRVSLFVGILAVLVSSLIGLAAG
                     LVAGYVGGAADTFLMRTVDVLLSVPLLPVLMVLAALWGKGLWQLVLILGLFSWMGTAR
                     TVRSMALSFREAPYVEGLRALGAPTFYILARHGIPTVLPLLLANIALGVPGVILSEAA
                     LAYLGLSDPRVISWGRMLHEAQSFGAFTTGAWWLLLPPGLGIALLCLAFMDLGRALEE
                     WADPRLKGGRA"
     gene            7224..8006
                     /locus_tag="PLY83_03185"
     CDS             7224..8006
                     /locus_tag="PLY83_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV29075.1"
                     /translation="MIEIEGLSVSYRSGKKEVQALSGVSLSLPSGQITGLVGESGSGK
                     TTLVMAILGLLPPEARVAGRILVDGADVVGMAENERDRFRWIRVALVPQGSQNAFNPV
                     LSIGSQVAEPLRVHRGLGRREAEKRARMLLDEVGLGSKASERFPHELSGGQRQRAALA
                     MALSCDPAFLLADEPTTALDVLIQAEIADLLKRAVRERKMGMALVTHDLPLASGLCET
                     ICVLDGGRLVETLPAKDLARGAKHPRTRALVAALLEMEGNLS"
     gene            8003..8929
                     /locus_tag="PLY83_03190"
     CDS             8003..8929
                     /locus_tag="PLY83_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV29076.1"
                     /translation="MSALLEIRGLCVSFRSRGGEHPALRGVDLDLGKGESLAVIGESG
                     SGKTTLLRAALGLVRPQAGTVRLFGRDLAAAGRRERTELLRRCGLMPQDPYGAVPPTL
                     SVREAVLEPLLLVQGFRSTPGSALKTEALLREWGLPETGLWDARVSRSLSGGQRQRVC
                     GARAMALDPELFLADEPTSMQDASLRGEIVAILERRVSSGMGLVLVTHDLILARKAAR
                     EGMVLYRGTAVEKGPTALLLEEPLHPYTAALARALPRLGRAISPPPTGRPAAGGLGCP
                     YSERCDRADAACREAPPLREVEPGRFVACHQV"
     gene            8958..9449
                     /locus_tag="PLY83_03195"
     CDS             8958..9449
                     /locus_tag="PLY83_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HOV29077.1"
                     /translation="MMVPENRRQLPGFLARGALVAALYALLVLAFAPLSFGPVQFRVA
                     EALTLLPWLFPEAILGLFAGCLVSNLFGGLGIIDMVFGSLATLVAAWLTRRMPNVFWA
                     AVPPVLVNGLVVGTYLSLLLDVPLASTILYVALGEAGVCFLLGVPLATLLTRILKERK
                     TRA"
     gene            complement(9492..>11570)
                     /locus_tag="PLY83_03200"
     CDS             complement(9492..>11570)
                     /locus_tag="PLY83_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29078.1"
                     /translation="AAMGEISSFTFNGFGSADQVGPGESSTPDGRPDARFSVSITGAG
                     ALAGVALRAEDGNGTWDTNPGNSAWGMRVRDGSGNLLTEANGRLPMVPFLLGINLEIS
                     VADNGDIARGGRFTLTARFLDNSEARSTITVPPSITATTARILSAQWMERANRDLTGR
                     DEILRGDGRPDERIRLELEGEGTLTEAEVRSLVEGRPTSAWDTIPGNGRWAMAVVRGR
                     ETLNNSDGSIEIPLSGKTVLDLWLSDDGSIEQGKTRYEVILRFADGRILRAPVAALAA
                     GTGPTQAEAILQATMMGEGNRDLVGRDENRAGNGKPDWRIDVKLDHPGTIIGMKVANV
                     KGPGGEWDTLPGNGRWLLAVTRTNGEILNRSDGAIRIPVTEPSAFILWLEDNGSLGNP
                     ETRSRITVVYDDGRILEEDIRPAPVSGTTGEGALLEAALAGVGSFDYVGPWEKLEGNL
                     NRDYRFDIRFSKKGTLTGVRIVNLTRGGEWDTIPGNGRWLTAVTRPGGGVLNASDGSV
                     RIPVDGTTALQLWVEDNNTLADPASAWRITLAFSDGQLLEKEIGTGSATTLPAVEKRE
                     LQLSKPQASASDFVGPYDRLLRNGRNDWVFTLKIRGRGRIQSLSLRNIGLRGVWDTVP
                     ANGRWTMAVRVPGGALLNRSDGSVSFPVPPNRTLQLFVENNGTLQLAASRYELVATWD
                     DGTTTTVRVP"
     assembly_gap    11571..11670
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     regulatory      11819..11925
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            12042..12542
                     /gene="thiT"
                     /locus_tag="PLY83_03205"
     CDS             12042..12542
                     /gene="thiT"
                     /locus_tag="PLY83_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HOV29079.1"
                     /translation="MKPNVKVMVEGALAAALTVALSYVKLWEMPQGGSITLESIPLLI
                     FSLRRGLRPGAGAGAVAGLLQMLLGGYVVHPLQALLDYPFAFGALGLAGVARKPLWAG
                     VALGSFARFACHVLSGAVFFGSYAPKGTNVWIYSVTYNGSYMLPNLLIAVIVVALLWP
                     RLSRIS"
     gene            complement(12609..13361)
                     /gene="pssA"
                     /locus_tag="PLY83_03210"
     CDS             complement(12609..13361)
                     /gene="pssA"
                     /locus_tag="PLY83_03210"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOV29080.1"
                     /translation="MKTKRIRRSPRAIPFRKIIPNMITSGNILSGILSMILTLQGHYH
                     PAAWLIMAAVFFDAMDGKMARSLGGSTEFGVELDSLADAVSFGAAPALLFYAVFLWGW
                     LGIVGALSAAFFGLCGALRLARFNVFHAPGPFQGLPIPAGGLFLASLVMGGLSVPPAM
                     AAALAVATGLLMVSSIPYGNLKRLRSGNVNRLKLAFLFALTVALIVTLQGKAPLAALC
                     IYIFSGFFRFDWRKWLAIDPAELEEEEIEAEA"
     gene            complement(13336..13977)
                     /locus_tag="PLY83_03215"
     CDS             complement(13336..13977)
                     /locus_tag="PLY83_03215"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HOV29081.1"
                     /translation="MRLARDGIAAISACAFMAAGGWLIARPLGVFFLFVLLLLLWFYR
                     DPDRKAPEDERLWVSPADGKVMEIGHAEHPYTGRALCIGIFMSPLDVHVNRAPTCGTV
                     EFLDYVPGKRLAAYHPKASLENERFYVGMQTSRGKTLLVQIAGLLARRIVSRVRKGEA
                     LTRGQRYGMIKLGSKVEVYLPASVRPLVAVGDRVRAGETPIGGPADEDEKDQA"
     gene            complement(13981..16461)
                     /gene="gyrA"
                     /locus_tag="PLY83_03220"
     CDS             complement(13981..16461)
                     /gene="gyrA"
                     /locus_tag="PLY83_03220"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOV29082.1"
                     /translation="MAEFTETDHSSPEKGNPAGLGRVVPLPLEDEIKHSYLDYAMSVI
                     VGRALPDARDGLKPVQRRVLYAMRELGLSHNQPYKKSARVVGETMGKYHPHGDSAIYD
                     TMARMAQDFSMRAPLVDGQGNFGSIDGDPPAAMRYTEARLSQLGEEILADIDEDTVDW
                     LPNFDESLQEPAVLPARLPNLLVNGSSGIAVGMATNIPPHNLGEVVDALCALLDNPES
                     DLSDLIGLLPGPDFPTGGIIMGRDGIVEAYRTGRGKIVVRGRTSIDDGKRGKKAIIIS
                     EIPFMVNKTALIEAIADGVKSGHLDGITDIRDESDRRGMRIVLELHRDANPDVTLKQL
                     FSRTSLQTTFGVINLALVDGRPRELSLLEMLWVYLDHRRAVIRRRTAFRLRKAEERAH
                     LVEGLVRALDMIEAVISLIRASKDTQTAREGLVEQLGFTEPQAQAILDMRLQRLTGLE
                     RRKLEEELAELLADIERYQTILGNPAVLDAVVREELVEVKRKFATPRRTEISSVLEDV
                     SVEELIPESDIVIVLSRDGYVRRSPLQDFHTQSRGGKGVRGAVLRGEDEIAIMTCTTT
                     HRDLFLFTSRGRVFAIKGLSVPEPRSGKGKPIRDLISLEENERIVAMKDSSLRGAAYV
                     FFVTERGLAKRLPVSELENLTRAGRRVLGLSEGDTIARVRFTRGDNELLLTTAQGQAL
                     RVREEEFRPQGRLAMGVRGIRLAPGDRVVGCVVLQDTLDILLISEKGVGKRTCCLEFP
                     VHHRGGGGIRAMRLGDRTGDLVGSWGVLPEDEVVVISSRGRMVRQSVSGIPRLSRTAT
                     GSILVRLDEGDSVSDVSVVVETACDVEV"
     gene            complement(16575..17102)
                     /locus_tag="PLY83_03225"
     CDS             complement(16575..17102)
                     /locus_tag="PLY83_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOV29083.1"
                     /translation="MEEFENPVIQAILARRSIRRFQQKPVEPEKVDCLVECACAAPTA
                     ANSRPWHFIVVDERAVLDALGEAHPYGKMLYQAPLAVVVCGDPEKSDFARLYWEEDCS
                     AAMENLQIAAHALGLGSVWLGVRHSTEREQAVRHILGIPQKIAVLGIAAIGYPDEVKP
                     PHEGIDPGSLHLNRW"
     gene            17316..19019
                     /locus_tag="PLY83_03230"
     CDS             17316..19019
                     /locus_tag="PLY83_03230"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOV29084.1"
                     /translation="MGKTQSGFEDFRLRPELSQALDERGFTNPTPVQERVLSDENALL
                     GDLIVQAKTGSGKTLAFALPILNEMDAGESLPRVLVLSPTRELALQTAQEFESMAKPL
                     RIRIAALVGGMDMEAQLRRLRVGAGVLVGTPGRVLDHIRRKAFDCEPIHTVVLDEGDH
                     MLDMGFRDELEAILDSLPSRERTWLFSATMPEEVVKLSSRYLSAPRWISLVEDTASHE
                     DIAHRAYLIPSGRRVESLINVLLWEAPERALVFCPTRIETAEVAERLQGAGFSALPLH
                     GDMGQKERNTALGAFRAGRVRILVSTDVAARGIDVEGISHVIQLGLPGTLDNYVHRSG
                     RTGRAGNEGLNLSLLTPRDARTLKSLLRGHSLSIEWLSVPDSHEIRLLERGRLDRELA
                     ECEEQPSEMYLEWAREILGRNEAECVVARLLERLFAQRSSGFALRRELDREMEAEERR
                     RFAKREGKPAARAGVRREETGGATRLRLEKGRADGVEVGALLGILCRALKVDRREIGN
                     IRLRDRFAEVELGERAARHLERYRKELEKEGFLSPEGSFSGRKPRPASERERASHRPA
                     L"
BASE COUNT         3130 a         5909 c         6354 g         3622 t
ORIGIN      
        1 ctgccgctgt gggggatctg cctggggctg caagtcatgg cccttgctgc aggggcccgg
       61 acgtacaagc tgccctacgg tcatcgcggc gtgaaccacg ccgtggggga agcggagggc
      121 cccaggggct ggatcacgag ccagaaccac ggctacgcgg tagacgcgga aagcctttcc
      181 gggaccggct ttaccgtttc gatggtccac ctgggggacg ggagcgtgga gggaattgtc
      241 agcgagcgtc ttcgggcctg gtcggtgcag tttcaccctg aagccttcgc aggaccgaag
      301 gacacgggat ttcttttcga cgaatttctg aaggggctga actagatgaa atcttcgatg
      361 gaaaaatcca tccagacggt cctggtgctg ggttcgggcc cgatccggat cggccaggcg
      421 gcggagttcg actattcggg aagccaggcc tgccgggcgc tccgggagga gggcgtgaaa
      481 gtcatcctgc tgaacagcaa tccagcgacg atccagaccg accccgggct ggcagaggtg
      541 acgtacatcc ggcccctgac cccggaggtg gtcgaagaca tcctcaaggc ccaccgtccc
      601 gacggggtcg tggcaacact cggagggcag accgcgctga acctcctcgt cgagctgaac
      661 cgccagggag tctgggagcg gtacgggacg ctgcctctcg ggacctctcc cgcaagcgtg
      721 gaacgggcgg aagggcgcga acccttccgc gacctgatgc ggtccatcgg ggaacccgtc
      781 gtggaaagcc ggatcgtgac ggaaccccag gcggctctcg agtttgccgc gcatgtcccg
      841 ttcccgctcg tgatccgccc ggatttcacg ctggggggaa cgggaggcgg agtggcgttc
      901 gacgagggct ctctcgtgga gagggtccag gaaggcgtgg cggcgtcccc tgccggccgg
      961 gtgctggtgg agcgctacct ctcgggatgg tacgaggtgg aaatggaagt cgtccgggac
     1021 ggcttcggca acgccctctg cgtctgcggc atggagaacg tcgacccgat gggaatccac
     1081 acgggggaca gcatcgtggt cgctcccgtg ctgacgctga gcgacagcca gtggcagaac
     1141 cttcgacggg ccgggctgaa gatcgtgaac gccctcgaca tccggggggc ctgcaatgtc
     1201 cagttcgcgg tttcgccggg catggaggaa tacgccgtga tcgaggtcaa cccgagggcc
     1261 agtcggtcga gcgccctggc cagcaaggcc acgggatatc ccatcgccag gatggcggcg
     1321 aaaatcgccc tggggaaatc cctcgcgaag atgcccaacc cggtcaccgg ggtcggcagc
     1381 gctctttcgg aacccgccat cgactacgtc gccgtcaagt tcccgcgctg gccgttcgac
     1441 aagttccccg acgccgatcc gacgctgggg acacgcatga aatcgaccgg ggaggtcctt
     1501 gccatcggtc cgaatttccc ccaggcactg ctgaaagcca tgcgttccgt ggagtggggc
     1561 ggagggctga ggcccgcggg atgcgcccaa ctgtcggaac gggctctatg ggctgaggtc
     1621 cgccgtccta cacaccggcg cctgtggttc atcctcgagc tgctccgacg gaaagaggat
     1681 gttgaatccc ttgcgcaggc cagcgggatc gcgccctttt tcctccgcca gatggcaaag
     1741 gtcgtcgctg ccgagcgaaa cctggaaaaa cggggaatcc aggacccgga agccctggcg
     1801 gaagcgaaat ggctggggct gtcggaccgg gatatcgcgg ctcttttgga aacgaaggag
     1861 gaagcggtcc gggaagttcg cctttcccag gggatcgggc agggctttcg cgaggtggac
     1921 ggctgcgcgg gcgagacgcc ggcaggttcc cggtacctgt acggggccta cggcgtgtcg
     1981 agtgacggca ccccggaagg gaacactccc gcggtcgtcg tgctgggttc ggggccgatc
     2041 cggatcgggc agggggtcga attcgactac tgctgcgtca aagccgtgga ggcgctgaaa
     2101 agccttggaa tccgggccgt gatggccaac aacaaccccg agacggtcag cacggatcac
     2161 gatctctccg atgccctcta cctggagccg ctgaccgtgg aggatgttct gccgatcatc
     2221 cggagggaga gcgctcgggg cgttttcgct tccttcggcg ggcaaaccgc gctgaagctg
     2281 ggaatggagc tggcggaggc gggggtcccg ctgctgggta ccgggctggg ggcgatcgag
     2341 gcggcagagg accggggcca gttttctacc atcctgaagg acctggggat ttcccagccg
     2401 gaaggcgggg ccgtccggtc cgtcgaggag gcgctcaagc tggcggacag gctcgggtac
     2461 ccgctcatgg tccggcccag cttcgttatc ggggggctgg cgatggcgct ggtggaaagc
     2521 gagagcgatt tgaggtcggt gctgtcccag gcgttcgagg ccatgcccgg gcaatcggtc
     2581 ctggtagacc gctttgtttc cgggacggag tacgagctgg acgcggtctg cgacggggcc
     2641 gacatcgtgg tcccggggat tttcgaacac ctggaccccg ccgggatcca ttccggcgat
     2701 tcgatggccg tgtttcccga cctctccctg aacgagtcca ggcggcagga gatggtcaag
     2761 atcgcttctt ccatcgcgga ggccctggat gtccgggggc tcctgaacgt ccagttcgtc
     2821 gagaacgggg gacggctgac ggttatcgaa gccaatccga gggccagccg gaccgtaccg
     2881 atcgccggca agctgacggg tgttccgctg gtggaactgg ccgtccgggt tgccctgggg
     2941 cagcgccttc gggaccttgg tgtcccgctt gggctcttgc cgccccgcgg ccccctggga
     3001 gtcaaggtgc ccgttttctc cacggagaaa ctccccggcg tggacagccg gcccggtccc
     3061 cagatgcagt ctacagggga atccctggga gtcggggagt gcgtggcgac ggctctctgg
     3121 gaaggcctgg gcggggccgg atgggtgctt ccggagaagg gccggatcct ggtttcggtg
     3181 aacgacgctt ccaaggccgg ggcctgcagc gtcgccgcga cccttttcgg cctggggtgg
     3241 gagatcgaag ccaccgaagg cacggccggg tacttccgtc gttggggcct gcccgtcacc
     3301 acggccgaga aaggacaagc cctcgtgaat gcgcttcggg ggcgccgctg ggatcttgtc
     3361 gtgaatgtcg cggggtcccg gctgggggcc ctcaaggacg ggtacgcgat ccggagggcc
     3421 gcgatcgaaa cgggaatccc ctgcgtcagc agtctcccgg tggcctccgc cctcgcgctg
     3481 gccatggcgg tccggcatgg taacatgaaa ccctaggagt ggattccttt cctgggggtg
     3541 tttgcgatgg gcggattcaa gcggtggctg gcgggagcgt tgggtgccct ggtgatggcg
     3601 gtgcccctgg cccttccggc gcagggagcg ggcgtggacc tggaacggat ccgggggccg
     3661 aaggtccggg acctctacct ggtcgtggtg cgggacccgg atgcggcgat cctggccctc
     3721 gaaaggggaa agctcgacat cctgggggat gtcacccgcc cctccgatat ccgcaggctc
     3781 tcgaaggacc cgcggttttc cctctccatg gccgagaatt ttcacatggc gcaggtcggg
     3841 ttcaacctca ggaaattccc ctggaatgtc caggagctga gaagggcggc ggccatggcc
     3901 atcccgaggg accgcctggt ccgtgaactc ttttcggggt attcggcccc gctgacgacg
     3961 tttcttcccc cggtttcccc ctattgcgac aaaagcgcgg cggctcccgg cctggacgtg
     4021 gaaggggcgc gtgccctgct gaagaaggcg ggatggacct ggaacgcttc cgggatcctg
     4081 gtccccccgg ggtcgaagac cccgctgccc ccggcgacga tcctgtctcc cacggcgggg
     4141 gtggctccca cgaccgcgga gctggccgac cggatcgcgg cggctttgag ggaaatcggc
     4201 atcccggtca aggccgagcc gctcgatttc gcggcgatgg tccagcggat cgacgagcgg
     4261 aaattcgacc tctacctcat ggcctggcag ctcggaaggg atcccgacag cctttactcg
     4321 ttctaccact cctcgatgga cgtccgggga ggctacaacc agaccggcct caaggacgcc
     4381 gtgatggacg gcctgctgga gtcgctccgg tattccccgg acgagcccgc ggcccgcaag
     4441 gcagccaggg aggttcagaa agccctggcg gaccggcttc cgatcatccc gatctacacg
     4501 aggtactccg tggccgcggt ttccagggaa tggaaagggg ccgttgccac taaggcttcc
     4561 gcggctgaca acagctggag ccttctttcc atggagccgg catcgggacg ggagaagccg
     4621 ctctactggg ccctgggcga cgagccgcgg acgctgaatc ctttcaccgc cagcacggcc
     4681 acggactggc aggtcctggg cctgctttac gacacgctgc tggcggtgag cccgaacgac
     4741 ctttccgacc tgccgtggct ggcccgttcc tggaaggtgg aaacggtagc caaagcggga
     4801 gcgccgaaga cgcgcctgac cttctccctt cggggggacg tcgtctggca ggacgggaag
     4861 ccttttaccg ccaaggacgt gaaggcgacc cttctctttc tgaagaagca cgcgatcccg
     4921 cgctattttg attccgttcg ggacatcgag tccgtcacgg ttccggatga ccggacggtc
     4981 gtggtgacga tgaagaacgt cagctactgg cacctgcact cgatcggcgg ccttcccctt
     5041 ttcccggcgg ccctcctgga ccggatccgg gactggcgtt cctggcagcc ctcgcgaact
     5101 cccctccccg gtaaaacggg gctgaccgaa ctgtacggaa cggggccgtt cgttttcagg
     5161 gaatcgaaag ccggcgattt tgtccggttt tcccgcaacg agcgtttctg gttgattccc
     5221 aggtgaggcc cgtgaaaagg tactggaccc ggcgcctggg gggcgccgcc tgtgtcgtgg
     5281 cgatcgtcct cgtggcaaat ttcttcctct tccggctgat gccgggggat cccgtggcga
     5341 ccgtcctgga tcccaggttc tctcccgaag cgaaagcgca gcttgaggcc ctctacgggc
     5401 tggaccagcc cctgggggtc cagttccttc tctacctgag gagaatggcc acgttcgatt
     5461 tcggcctttc gttctccacc cagcggcccg tcctggacga gatcctggaa cgccttccaa
     5521 acacgctggc ccttctggtt cccgcctacc tgctttcggt cctgctggga accgccctcg
     5581 gcgtgattgc ggcccgcaga aggggacagc tggcggagaa ggcggtcctc gcggcggggg
     5641 cggtttcgtt ttccttcccc tcgtttttcg tccagatggt gctcctgctc ctttttgcct
     5701 acgtgtttcc cgttttcccc ctgcgaggca gtctttccat ccctccgccg gaggggacct
     5761 ggcgaatcct ggcagaccga atctggcacc tggccctgcc ggttttctcg ctggtcctcc
     5821 tggggtttgg cgggtgggcc ctgacggtgc gcaacctggt cgtgaaagtc atgggggagg
     5881 actttatgat cctggcccgc gccaagggca tcggagagcg ccgcctgctc tgggggcacg
     5941 ccctgagggc tgccctgccc ccgctcttga cgctctttct gctttccctc cccggagtgg
     6001 tgtcgggagc cgtgctgacc gaaacggtct tttcccttca cggcgtgggc cgcttcctgc
     6061 tggattccgt cctcgggcag gactacccgg ctgcgggagg ggcattctac ctcctcacgc
     6121 tcatcacggt ggggagcaac ctcctggccg attttctcgt ccagctcgcg gacccgagga
     6181 tcaaaacggg gggggaggag tcccggtgag gaaaatgcgg tggagcgcat gggtcctggc
     6241 ggttttcctt ttctgcgggt tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnccgctgga cgctggacct cgatgcgcgg gacatggcgt ttcggatctc cctgggcctc
     6421 tcgcccttcg acgaatctct ccggggcctt ttcgacaaac acggcacgat ccgggtccgg
     6481 atcgaaagcg atgtcccggt ggagcgttct tctcttatgg ttcagatccc cggtgaacgg
     6541 tgggggcttt ttgggacgga ccaaaggggc cgggacgtgt tcctcctttt tctctacggg
     6601 atccgggttt ccctgttcgt gggcatcctg gccgtgctgg tttcctccct gatcggcctg
     6661 gctgccgggc tcgtcgcggg atacgtgggg ggagccgcgg atacattcct gatgaggacc
     6721 gtggacgtcc ttctttcggt tcccctcctt ccggtcctca tggtcctggc ggcgctctgg
     6781 ggcaaggggc tttggcagct tgtcctgatc ctgggactgt tttcctggat gggaacggcc
     6841 cggactgtcc ggtccatggc gctctccttc cgggaggctc cctatgtcga ggggcttcgg
     6901 gcgctgggag ccccgacttt ctacatcctg gcccggcacg ggattcccac ggtccttccc
     6961 ctgctgttgg cgaacatcgc cctcggggtc ccgggggtga tcctttccga ggcggcgctc
     7021 gcctacctgg ggctttccga tccccgcgtc atctcctggg gacggatgct ccacgaggcc
     7081 cagtcgttcg gggcattcac gacgggagcc tggtggctcc tcctgccgcc gggtctcggc
     7141 atcgcccttc tctgcctggc gttcatggac ctgggaaggg ccctggagga gtgggcggat
     7201 ccgaggctca aggggggacg ggcatgatcg agatcgaagg actttcggtt tcctaccgtt
     7261 ccggcaagaa agaagtgcag gcgctttccg gggtgagcct ttccttgccc tcgggccaaa
     7321 tcaccgggct cgtcggagaa tccggcagcg ggaagacgac cctggtgatg gcaatcctgg
     7381 gcttgctgcc tcccgaggcg cgcgtcgccg ggcgcatcct ggtcgacggg gccgatgttg
     7441 tcgggatggc ggaaaacgaa cgggaccgct tccgctggat ccgggtcgcc ctcgttcccc
     7501 aggggagcca gaacgccttc aatccggtcc tgtccatcgg gtcccaggtc gccgagccgc
     7561 ttcgcgttca ccggggactc gggcgccggg aggctgaaaa gagagcccgc atgctcctgg
     7621 acgaagtggg actcggctca aaggcatcag aacggtttcc ccacgaactt tccggggggc
     7681 agcgccagcg tgcggccctg gcgatggcgc tctcctgcga tccggccttc ctgctggccg
     7741 acgaaccgac cacggcgctg gacgtgctca tccaggcgga gatcgcggac cttctgaagc
     7801 gggccgttcg ggagaggaag atgggaatgg ccctggtgac gcatgacctg ccgctggcgt
     7861 caggcctctg tgaaacgatc tgcgtcctcg acggcgggcg gctggtagaa acgctaccgg
     7921 ccaaggacct ggcccggggg gcgaaacacc ccaggacgag agccctggtg gccgcgctgc
     7981 tggaaatgga gggaaacctc tcatgagcgc gctgctggaa atccgggggc tttgcgtgtc
     8041 cttccgcagc cgcgggggag agcatccggc cctgcgggga gtcgatcttg acctgggcaa
     8101 aggagaaagc ctggccgtga tcggtgagtc tggaagcggc aagacgacgc tcctgagagc
     8161 ggccctgggc ctggtgcgac cgcaagcggg gacggtccgc ctgttcggca gggacctggc
     8221 tgccgcaggc cggcgggaac ggaccgaact gctgcgccga tgcggcctga tgccccagga
     8281 tccctacggg gccgttcccc cgaccctttc cgtgagggag gcagtcctgg agccgctctt
     8341 gctggtgcaa ggcttccgat caacccctgg aagcgctctt aaaactgaag cgctgcttcg
     8401 ggagtgggga ttgcccgaaa cgggtctctg ggacgcgcgg gtgtcccggt ccctttcggg
     8461 agggcagcgg cagagggttt gcggggcgag ggccatggcc ctggacccgg aactcttcct
     8521 tgccgacgaa cccacgagca tgcaagacgc ttcgctcagg ggggagatcg tggcgatcct
     8581 ggaaaggcgc gtatcctccg ggatggggct ggtcctggtg acgcacgacc tcatcctggc
     8641 ccggaaagcc gcccgtgaag gaatggtcct ttacaggggg acagcggtgg agaagggacc
     8701 caccgcgttg ttgctcgaag aaccccttca cccttacacg gccgctttgg caagggctct
     8761 gccgaggctg ggcagggcga tctctccgcc gcctacggga agacccgccg ccggtggcct
     8821 ggggtgtcct tactcggaac gctgcgatcg ggccgacgcg gcctgcaggg aggctccgcc
     8881 tctaagggaa gtcgaaccgg gccggtttgt ggcttgtcat caagtttgag gcggatttcg
     8941 atacgggagg gatctggatg atggttcctg aaaaccgccg gcagctgccg ggatttcttg
     9001 cccggggcgc actggttgcg gctttgtacg ccctgctggt gctggcgttc gcgccgcttt
     9061 ccttcgggcc ggttcagttt cgggtggccg aagcgctaac gctgctcccc tggcttttcc
     9121 ccgaggcgat tcttggttta ttcgcgggct gcctggtttc caacctgttc ggcggcctgg
     9181 gtatcatcga catggttttc ggcagcctgg ccacgctcgt tgctgcctgg ctgacccggc
     9241 gcatgcccaa cgtgttctgg gcggccgttc ccccggtcct ggtcaacggg cttgtcgtcg
     9301 gcacctacct gtcactcctg ctcgacgtcc ccctggcctc aaccattctt tacgtggcgc
     9361 tgggcgaagc gggggtctgc ttccttctgg gtgtgcccct cgccacgctt ttgacccgga
     9421 tcctgaagga aaggaagacg agggcatgaa aaaggggctc ctgaaaggag ccccttaacc
     9481 gccggttttt ttcagggaac gcgaaccgtg gtggtcgtcc cgtcatccca ggtggccacc
     9541 agttcgtagc gggaagccgc aagctgcagg gtcccgttgt tttccacgaa gagctgcagg
     9601 gtccggttgg gcggcaccgg gaacgaaacg gacccgtcgg accggttcaa aagcgctccg
     9661 ccgggaacgc gaaccgccat cgtccaccgg ccgtttgcgg gaaccgtgtc ccagacgccc
     9721 cggaggccga tgttcctcag gctaagggac tggatcctcc cgcgtccgcg gatcttcagg
     9781 gtgaagaccc agtcgttcct gccgtttctc aaaagccggt cgtacggacc gacgaaatcg
     9841 ctggcggaag cctggggctt cgacagctgc agttcccgtt tttcaacggc cggcagagtc
     9901 gtcgcagaac cggtcccgat ttccttctcc agcagctgac cgtcgctgaa ggcgagcgtt
     9961 attcgccagg ccgaggcggg gtcggccagg gtgttgttgt cctcgaccca gagctgcagg
    10021 gccgtcgtgc cgtccaccgg gatccggacc gatccgtcgg aagcgttcag gacgccccct
    10081 cccggccggg ttacggctgt cagccagcgg ccgttgccgg ggatcgtgtc ccattcgccg
    10141 ccccgggtca ggttgacgat ccgcacgccc gtcagcgtcc cctttttcga gaaccggatg
    10201 tcgaaccggt aatcccggtt caggttccct tccagcttct cccaagggcc gacgtaatca
    10261 aagcttccaa ccccggccag ggccgcctcc aggagcgctc cttccccggt ggttcccgaa
    10321 acgggcgccg gccggatgtc ctcctcgagg atccttccgt cgtcgtagac caccgtgatc
    10381 cgcgagcggg tttcggggtt tccgagagaa ccgttgtcct ccagccagag gatgaaggcg
    10441 gacggttccg tcaccgggat gcggatcgcc ccgtccgatc ggttgaggat ttcaccgttc
    10501 gtccgggtca cggccaggag ccagcggccg tttccgggaa gggtatccca ctcgccgccg
    10561 gggcccttca cgttggcgac cttcatcccg atgatcgtcc cggggtggtc gagcttcacg
    10621 tcgatccgcc agtcgggctt tccgttcccg gcgcggtttt catccctgcc gacaagatcg
    10681 cggttccctt cccccatcat cgtggcttga aggattgctt ccgcctgtgt ggggcccgtt
    10741 cccgcggcca gggctgcaac cggtgccctc aggatcctgc cgtccgcaaa ccgaagaatg
    10801 acctcgtaac gggtcttccc ttgctcgatg gaaccgtcgt cgctcaacca caggtccagg
    10861 accgtcttcc ccgaaagggg aatctcgatg ctgccgtcgc tgttgttcag ggtttccctc
    10921 ccgcgaacga cggccatcgc ccaccggccg tttccgggaa tcgtatccca ggccgaggtc
    10981 ggcctacctt caacgaggct gcggacctcg gcctccgtca gtgttccttc tccttccagc
    11041 tcaaggcgga tccgttcgtc gggccgaccg tctcccctca ggatttcgtc tcttccggtg
    11101 aggtcgcggt tggccctttc catccattgc gcagagagga tcctcgcggt cgtggcggtg
    11161 atcgagggag ggaccgtgat cgtggaacgg gcctcggagt tgtccaggaa gcgggccgta
    11221 agcgtgaagc ggccgccccg tgcaatgtcc ccgttatccg cgacggaaat ttccaggttg
    11281 atccccaaaa ggaaggggac catgggcagg cgcccgttcg cctcggtcag gaggttcccc
    11341 gacccgtcac ggacacgcat cccccaggct gagtttcccg ggttggtgtc ccaggtcccg
    11401 ttgccgtctt cggcccgaag cgcgacgccg gccaaggctc ccgcaccggt gatcgaaacc
    11461 gagaaacggg catcgggcct gccgtcggga gtgctgcttt cccctggccc cacctggtca
    11521 gcggagccga atccgttgaa cgtgaagctg gaaatttcgc ccatcgcagc nnnnnnnnnn
    11581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gtccgattcc gctccaggga gatgcggcgg
    11701 ctgccatccg gaaaatcctt tcccgcctcg aggcggttct gaacccctcc gggagggtgc
    11761 gggtcaggtc tcgctttcaa taggcgttga caccgcctgg cggtgggagt ataagtctgc
    11821 atggcatcgg gggagtcgga attccggctg agaggaggcg aaggcctcga cccctggaac
    11881 ctgatccgga tcatgccggc gaaggaaaga tgcgagccct cttttttcac gcgtttccct
    11941 tctttcttct ccagcggttc ccttcccgag atgccgatct caggtcagct gccggttgcg
    12001 cccttgttcc ggcaaggacc ggaaggatgg aggagtcccc tttgaaaccc aacgtgaagg
    12061 tcatggtgga aggagcgctg gcggctgccc tgacggtcgc actctcctac gtcaagctct
    12121 gggagatgcc gcagggagga tcgatcaccc tggagtcgat cccgcttctg attttctccc
    12181 tgagacgggg cctgcggccc ggtgcaggag ccggagcggt agccggactg ctgcagatgc
    12241 tgctgggcgg atacgtcgtt caccccctcc aggccctgct ggactatcct tttgcattcg
    12301 gggccctggg ccttgccgga gttgccagaa aaccgctctg ggcaggcgtg gcgcttggga
    12361 gtttcgcacg gtttgcctgc catgtccttt cgggtgcggt ctttttcggg agctatgccc
    12421 cgaaggggac gaacgtgtgg atctattccg tgacttacaa cggaagctat atgcttccga
    12481 acctgctgat cgccgtgatc gtggtggcgc ttctctggcc gaggctcagc cgcatctcgt
    12541 agaaaaaggg cggccccggg agctcgatct cccggggccg ccctttccac tcttctttag
    12601 ttttccagtc aggcttccgc ttcgatctct tcttcctcga gctctgcagg gtcgatcgcg
    12661 agccacttcc tccagtcgaa gcggaagaac ccgctgaaga tatagatgca gagcgcggca
    12721 aggggggctt ttccctgcag ggtcacgatg agcgcgacgg tcagggcgaa aaggaaagcc
    12781 agcttgagcc ggttgacgtt cccgctgcgg agccgtttca ggttcccgta ggggatgctt
    12841 gaaaccatca aaagccccgt tgcaacggcc agggccgcgg ccatggccgg agggacggaa
    12901 agcccgccca tcacgaggga ggccaggaaa agccccccgg cgggaatggg cagtccctgg
    12961 aagggacctg gggcgtggaa cacgttgaag cgggcgagcc tcaacgcccc gcagaggccg
    13021 aagaaggcag cggacagggc accgacgatg cccagccacc cccaaaggaa aaccgcgtaa
    13081 aagagcaggg ccggagcggc cccgaagctt accgcgtcgg ccaggctgtc cagttcgacg
    13141 ccgaactcgg tgctacctcc aaggcttcgc gccatcttcc cgtccatggc gtcgaagaag
    13201 accgctgcca tgatcagcca ggcggcaggg tggtaatgcc cctgcagggt caggatcatg
    13261 gagaggatcc cgctcaggat gttgccgctg gtgatcatgt tgggaatgat cttgcggaag
    13321 gggatcgccc gagggctacg cctgatcctt ttcgtcttca tcggcaggtc ctccaatcgg
    13381 tgtttcgccg gcccgaaccc tgtcgcccac ggcgacgagg ggccgaacgg acgccggaag
    13441 atagacttcc actttcgaac caagcttgat catcccatac ctctgccccc gtgtcaaagc
    13501 ttcgcctttt cgcactctcg aaacgatccg ccgggccaaa agccccgcga tctgcacgag
    13561 aagggttttc ccccgggagg tctgcatccc gacgtagaag cgctcgtttt cgagggaggc
    13621 cttgggatgg taggcggcga ggcgtttccc cgggacgtag tcgaggaatt ccaccgttcc
    13681 gcaagtcggg gcacggttga cgtgaacatc caggggagac atgaaaatgc cgatgcagag
    13741 ggctcgcccg gtgtagggat gctcggcgtg gccgatctcc atcactttcc cgtcggcagg
    13801 gctgacccag agcctttcgt cctccggggc cttccggtcg ggatcacggt agaaccagag
    13861 aagaagcagg agaacgaaaa ggaaaaaaac accgagcgga cgggcgatca gccaccctcc
    13921 cgcggccatg aaggcgcagg cggaaatggc ggcgattccg tcccgtgcaa gcctcatccc
    13981 ttaaacctcc acgtcgcagg cggtttcgac gacgacgctg acgtcagaaa cactgtcccc
    14041 ttcatcgagg cggaccagga tggatccggt ggcagtccgg ctcaggcgcg ggatgccgga
    14101 aacgctttgc ctcaccatcc tgccccggct ggagatgacc accacctcgt cctcgggaag
    14161 aacaccccag gaaccgacaa ggtctcccgt gcggtctccc aggcgcatgg cccggattcc
    14221 tccgccgccc cggtgatgga ccgggaattc caggcagcaa gtccgtttcc cgactccttt
    14281 ttcgctgatc aggagaatgt ccagcgtgtc ctgcaggacg acgcatccga ccacccggtc
    14341 gccgggggca agccggattc cccggactcc catggccagg cgtccctggg gacggaattc
    14401 ctcctcccgg acccggaggg cctgtccctg ggccgtggtg aggaggagtt cgttgtctcc
    14461 ccgggtaaac cgtacacggg cgatcgtgtc gccttctgaa agtccgagga cgcgtctccc
    14521 ggcccgggtg aggttttcca gttccgatac cggcagccgc ttggccaggc cccgctcggt
    14581 gacgaagaag acgtaagccg cgcctctcag gctcgagtct ttcatggcca cgatccgctc
    14641 gttttcttcc agcgagatga ggtcccggat gggtttgccc ttgccgctcc tcggttcggg
    14701 gaccgaaagt cccttgatgg caaaaacgcg gccgcgggag gtgaagagga agaggtcccg
    14761 gtgggtcgtg gtacaggtca tgatggcgat ttcgtcttcg ccgcgaagga cggccccccg
    14821 gacacccttc cctccccggg actgggtgtg aaagtcctgg agcggtgagc ggcggacgta
    14881 tccgtcccgg gacaggacga tcacgatgtc ggattccggg atcagctcct cgactgaaac
    14941 gtcttccagc acggaggaaa tttcggttct ccggggcgtg gcgaactttc gtttgacttc
    15001 gaccagttct tctcgcacga cggcgtcgag aacggccggg ttgcccagga tcgtctggta
    15061 acgctcgatg tcggcgagaa gctccgccag ttcttcctcg agcttgcggc gctccagccc
    15121 cgtcaggcgc tggaggcgca tgtccaggat cgcctgggcc tgcggttccg tgaagccgag
    15181 ctgctccacc aggccttccc tggcggtctg cgtgtccttc gaggcccgga tcagggaaat
    15241 gaccgcctcg atcatgtcca gggcgcgaac cagcccttca acgagatgcg ccctctcctc
    15301 ggcctttctc aggcggaagg cggtcctccg gcggatgaca gcccggcggt gatccaggta
    15361 gacccaaagc atttccagca gggagagttc ccggggccgg ccgtccacga gggccaggtt
    15421 gattaccccg aacgtggtct ggaggcttgt tcggctgaag agctgcttca gcgtcacatc
    15481 ggggttggca tcgcggtgaa gttcaaggac gatgcgcatt cccctccggt cggactcgtc
    15541 ccggatatcg gtgattccat cgaggtgtcc cgatttgacg ccatcggcga tcgcttcgat
    15601 cagggcggtc ttgttgacca tgaaggggat ctcggaaatg atgatcgcct tttttccccg
    15661 ctttccgtcg tcgatggagg ttctgccgcg gacgacgatc ttgccgcgtc cggtccggta
    15721 ggcttccacg atgccgtccc gtcccatgat aatgcccccc gtggggaaat cggggccggg
    15781 caaaaggccg atgagatcgc tcaggtccga ctcggggttg tccaggagcg cgcaaagggc
    15841 atccacgacc tctccgaggt tgtggggggg gatattcgtg gccatgccga ccgcgatccc
    15901 cgagctcccg ttcaccagga ggttcggcag gcgggcggga aggacggcag gttcctgcag
    15961 cgattcgtcg aaattgggaa gccagtctac cgtgtcctcg tcgatgtcgg ccaggatttc
    16021 ctcgccgagc tggctgagac gcgcctcggt gtaccgcatg gccgccggcg ggtctccgtc
    16081 gatggagccg aagttgccct gtccgtccac cagcggggcc cgcatgctga aatcctgggc
    16141 catccgggcc atggtgtcgt agatcgcgga atctccgtgg ggatggtact ttcccatcgt
    16201 ttccccgacc acccgggccg atttcttgta gggctggtta tgggaaaggc ccagctcccg
    16261 catggcgtaa aggacacgcc gctgaaccgg cttgaggccg tccctggcgt ccggaagggc
    16321 tcgcccgacg atcacgctca tggcgtagtc aaggtaactg tgcttgattt cgtcctccag
    16381 aggcagggga acaaccctcc cgaggccggc cgggttcccc ttttcagggg aagaatggtc
    16441 tgtttcggtg aattccgcca ttggcagtga tcctccatcc cgaagcaatc gctccagttt
    16501 caagaaccgg gcgcggcctg aagcctgccg cgcccgcaac tagaccattt taacacagag
    16561 gcggcctgag gcgctcacca tcggttcagg tggagagacc ccgggtcgat gccctcgtgc
    16621 ggtggcttca cctcgtcggg gtagccgatc gccgcgatcc cgaggacagc gattttctgg
    16681 gggatgccga ggatgtgccg gaccgcctgt tcccgctcgg tgctgtggcg aacacccagc
    16741 cagacgctcc cgagtcccag cgcatgggcg gcgatctgca ggttttccat ggcggccgag
    16801 cagtcttcct cccagtagag gcgggcgaag tcgctttttt cgggatctcc gcagacgacg
    16861 acggccaggg gggcctggta cagcatcttc ccgtagggat gagcttcccc gagggcgtcc
    16921 aggaccgccc tctcgtccac gacgatgaag tgccaggggc ggctgttggc cgcggtcggt
    16981 gcggcgcagg cgcattcgac gaggcaatcc actttctcgg gctcgacggg cttctgctgg
    17041 aaccggcgga tgcttcgcct ggccaggatt gcctggatga cggggttttc gaattcctcc
    17101 acgggttttt tcactccctt caataaatca tgtcccacga tgaaatttta ccggaaagtt
    17161 cttccttttc ccaccgaaaa gtcgaaccgc ttgacaaggg ggagccttgg cggataattg
    17221 gaccgggttg tctgaaaagg gaggaacaat cagaaagtgg ccgtcccgaa aagacggcca
    17281 ctttttttga ttcttgggag gttgttttgg gaaacatggg aaaaacgcag tcaggatttg
    17341 aagattttcg gttgaggccg gagctgtccc aggctttgga cgaacggggg tttacgaacc
    17401 ccacccctgt acaggagagg gtgctttcgg acgagaacgc ccttctggga gatctcatcg
    17461 ttcaggccaa gaccggctcg gggaagaccc tggcatttgc cttgccgatc ctcaacgaaa
    17521 tggacgccgg ggagagcctt cctcgcgtcc tggtcctctc gcccacgcgg gagctggccc
    17581 tgcagaccgc gcaggaattc gagtcgatgg cgaaacccct gcggatccgg atcgccgccc
    17641 tcgtcggggg gatggacatg gaagcgcagc ttcgccggct gagggtaggg gcgggagtcc
    17701 tggtcgggac tccgggccgg gtcctggacc acatccggag aaaggctttc gattgcgagc
    17761 cgatccacac cgtggtcctc gacgaggggg atcacatgct ggacatgggc ttccgggacg
    17821 agctcgaggc catcctcgat tccctgccct cgcgagagcg gacctggctc ttttcggcga
    17881 ccatgcccga ggaagtggtg aagctgtcga gccgctacct gtcggcaccc cgctggattt
    17941 cgcttgtgga agacaccgcc tctcacgaag acatcgccca ccgggcctac ctgatcccgt
    18001 cgggcaggcg cgtggaatcc cttatcaacg ttcttctgtg ggaggccccc gaacgggccc
    18061 ttgtcttctg cccgacgcgg atcgagacgg ccgaggttgc cgaaagactc cagggcgccg
    18121 gcttcagcgc cctgccgctt cacggcgaca tggggcagaa ggagcgcaac acggcgctcg
    18181 gggcattccg tgccgggcgg gtccggatcc tggtttccac cgatgtcgcc gcaaggggga
    18241 tcgatgtcga ggggatctcc cacgtcatcc agctgggtct tcccggaacg ctggacaatt
    18301 acgtccaccg gagcggccgg acgggaaggg caggaaatga ggggctcaac ctctcccttc
    18361 tgactcccag ggacgccagg accctcaaga gcctcctgcg ggggcattcg ctgagcattg
    18421 aatggctctc ggttcccgat tcccacgaga tccgcctcct ggaacgcggg cgtcttgacc
    18481 gggaactggc tgaatgcgaa gaacagcctt cggagatgta cctggagtgg gcccgggaga
    18541 tcctcgggcg aaatgaggcc gaatgcgtcg tcgccaggct cctggagcgg ctgttcgccc
    18601 agcggtcctc cgggtttgcc ctccgcaggg agctggaccg ggagatggag gccgaggagc
    18661 ggcggcgctt cgcgaagcgg gagggcaagc ctgccgcccg ggccggggtg cgccgggaag
    18721 aaaccggagg cgctacccgc ctgcggctgg aaaagggacg ggcggacggt gtcgaggtcg
    18781 gggcccttct ggggatcctt tgtcgcgcct tgaaggtcga tcggagggaa atcggaaaca
    18841 tccgtctccg ggaccggttt gccgaggtcg agctcgggga acgggcagcc aggcacctgg
    18901 aacggtaccg caaggagctg gaaaaggaag gattcctctc tcccgaggga agcttctccg
    18961 gcaggaagcc gagaccggcg agcgaacggg aacgggcctc tcatcggccc gccctgtgaa
    19021 tacaaaatac gtttcgtcgg aaccggacat ttctgaatcg tacctttggg ggttaagctc
    19081 tgtgaggcct tatcaataca gggcttaacc tttattgtat ataatttttg tacaaatccg
    19141 cataaatggt tgcatcgtgg ttcagtttgt ccgataatcg tggagtttac gcatcgggtc
    19201 tttttttgtg aaaaa
//
LOCUS       DAOOVN010000019        19126 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_1962_length_19126_cov_13.487389, whole genome shotgun
            sequence.
ACCESSION   DAOOVN010000019 DAOOVN010000000
VERSION     DAOOVN010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 19126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19126
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1962_length_19126_cov_13.487389"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..1079
                     /locus_tag="PLY83_03235"
     CDS             264..1079
                     /locus_tag="PLY83_03235"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOV29085.1"
                     /translation="MQISMEAILIVLGFAAGLLGGWIGTGGCSVMLPVLHFWLGFPAP
                     VAVGTTLFAVIFTAISGGYGHFKQKNVDKHTAIWTGGAGIAGVLLGSWLFTFLSNKSA
                     LLGCLIGLVFLWPSFRMIWEGFLGFRNRKLTSGMAGTAQGQPIKKAPPVQLASFGAAV
                     GILTGIVGLGGGYALVPGLIYLFGMPVYVTMGTSLAIMIPMAIVGGAIKLSQGYVFLS
                     AALLLAMGTIVGAQVGAATIKRFKPYALKLLFGLYFLYVAVKFIGNFLGIPVW"
     gene            1306..2316
                     /locus_tag="PLY83_03240"
     CDS             1306..2316
                     /locus_tag="PLY83_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOV29086.1"
                     /translation="MRILKAGRWGAVAAGILSLLFAGVAFASQPVLNAYTIWSERYAN
                     EIFTAFTKDTGIKVNWMRFSSGELQARLEAEKANPQVDVVFGDLAEAFASGIPKGLFE
                     PYRPAGSEAIPSRFKDPKGYWTGVALDPICFMFNSKFLKEHGLKPPASWQDLLDPAYR
                     GKLQMADARTSGTAMYRILSLVQAMGENEAFAYQKKLNANVQIYTKSGAGGALPIARG
                     QAAGGIFFLVDALEVQQTGYDVTISYPREGVVAGIEAMALVKGARQPELAKKFLDWAS
                     TERMQRIYEASRINLIPSNPRVKVSSPGLDLSKVKILPLDLEWAGKNRERLVERWVNE
                     VL"
     gene            2332..4023
                     /locus_tag="PLY83_03245"
     CDS             2332..4023
                     /locus_tag="PLY83_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOV29087.1"
                     /translation="MAAGFGQTVRSGRARLDPLTLALLLVLWAGLAVFVLYPLARLLA
                     SVFAADGSASLENLAKVFRNATTRKALVNSLVLAGAVAVSGTFWGYLFAFSVTRIPLP
                     KAFRWFLKGVVVLPFVSPPFTSSIALTLALGPGGLLLRLFRVPDFNLYGFAGTWVAET
                     LAYFPIAFLALSAILEAIDPNLEEAAASLGGSRWRVFRTVTLPLSVPGIANAFLLLFG
                     ASLTDFATPLVMAGHTFPVLTTQAYLQITGMYDLRGGAAISFVLLLPALLVYLLQQSW
                     VERKSQVTVTGRVGPRSAPRGAGRIAGILVLSAVSVLTLFILFLYSIIFLGSFVKVWG
                     VDHGLTLKNYAYVFTVGWKAVRDTVLIAVAATVLGTVLAALTGYLSARREVPFRKALG
                     FVSLLNYILPGTVVGIAYVIAFNSGPLVLTGTMTILVALCVFRYDAVGIRSTEAALRQ
                     VDPSLEEASYSLGAGRAATFFRVTLPLVAPAVAAGARYLFVVSMTAISATIFLVSVRW
                     SLLTVRILECVTELLFAEAAAFSVVLILIVFLASGAILLLLRLAVPQSPGGLRRS"
     gene            4027..5109
                     /locus_tag="PLY83_03250"
     CDS             4027..5109
                     /locus_tag="PLY83_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV29088.1"
                     /translation="MSLGISLHQLSKRFARGKEAVPAVDGVSLDVEAKEMVAFLGPSG
                     SGKTTILRIIAGFERPTEGRVLIGGQDVTDLEANARGIGFIFQNYALFPHMTVFDNVA
                     YGLRAKRVPAGEIPRKVAEVLEMVGLKGVEKRFPNQLSGGEQQRVALARVFVLDPRVL
                     LMDEPLANLDASLRTRMLAEIRRLQRHLGTTCIYVTHDQKEALTLADRVAVLNRGRLE
                     QVGTPLELYEAPQNLFVADFIGQANLLRGVVKAVSADSAEISVCGRSLRVRRGRIETL
                     KSGDSAGIAVRPSAVSLGAAGEAFLQGRVESRIFAGDRMEYEIEVEEGGMVRAYGALP
                     GRGVVFSEGDPVGIHFDPGETMAFPL"
     gene            complement(5150..5632)
                     /locus_tag="PLY83_03255"
     CDS             complement(5150..5632)
                     /locus_tag="PLY83_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOV29089.1"
                     /translation="MMNLEAERLLDRIGLEILSALQEDGRITFSELGKRVGLSSPAIS
                     ERVRRMEEAGIIKGYRAIVDPEKMGYPITAAIRLSSSRERQEELLDFLRERKEVLTCE
                     RVTGADSYLLKVIVTNVSHLEALLDSLSKFGQPVTSIVLSVPIDYRPIQKWHSGDERL
                     "
     gene            complement(5809..6423)
                     /locus_tag="PLY83_03260"
     CDS             complement(5809..6423)
                     /locus_tag="PLY83_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOV29090.1"
                     /translation="MLAAFVSFLVNTIGHMGYPGIVALMFLESSFFPFPSEVVVPPAG
                     YLAARGQMHLSLVIASGILGSLLGALFNYWISLRFGRPFFLRYGRYFLVSEKSIEKAD
                     RFFASHGHISTFVGRLLPGIRQYISLPAGLARMNIPLFLFFTGLGSGIWVVVLALVGF
                     WLGGDETGMTAALHRITILLAGFCLLVLGAYVLLKRRARAGTTD"
     gene            complement(6449..7780)
                     /locus_tag="PLY83_03265"
     CDS             complement(6449..7780)
                     /locus_tag="PLY83_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOV29091.1"
                     /translation="MEAQQNELFFWTLWWGWWLLQAGLYTLFAFLFLDAAYQFVIGFR
                     GWWSARPLPAAKTFRRFAILIPAHNEAAVLDGLLESLNLQEYPRERFDVYVSCDNCSD
                     NTADVAKSHGAIALIREDKTKNGKTWNVRWALQRIRLEDYDGLVMFDADNLAAPDFLA
                     RMNDYMEAHPDAEAIQGCLDVKNPDDNWLTRAYALAYWYTNRFWQLARSKWGLSCTLG
                     GTGLVVKSATMRRLGWNMQSLTEDLELSTRLILSGKRVHWNDHARLFDEKPQSLKVSW
                     RQRTRWMQGHYWVCWHYVFAALKRFAQTRRLQYLDLALYLMAPVKACVSLIVMIAGMI
                     YTLVNNAILFPTLGAKVPETALEWAFFISVPFLMILAYCAWVDVVGPSKRSGRLVLKY
                     VSAVPAYFLFGLTWIPILFRAAFMASSQAVWVKTEHTRRISIEQLSARRSA"
     gene            8071..9948
                     /locus_tag="PLY83_03270"
     CDS             8071..9948
                     /locus_tag="PLY83_03270"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HOV29092.1"
                     /translation="MARIESLKQRFDSNELALTGFLALAVSLKFVGLNNTLMRGSLED
                     PLTLLALMGTSLALASLPLLLPRRFRGAGMLFVDFWLTVLVWTDLLSLRYYSDLFTLR
                     NLGLGTQLSAVADSVVALMEPKDALLWIDFPVLGYLAFRVTRRFRSLPALDWGRAGAL
                     LLLTAILLVPFGLRVQRFEKTCPGALASMWDRPAVALSLGSLNYHLADLLSVATERFR
                     VRKPGTDLEEVAGWFQERPGAGVQRYHGIASGKNLIVIQVEALQAFVLNRSVGGVEIT
                     PNLNRLARRGLNFTETYHQTAMGNSADAEFLVNTGLFPAPSGVAYTRYAGNEFNSIAS
                     ALKAKGYSTIALHGDRPGFWNRARMYPSLSFDRFVSNRDFQVDSIIGLGLSDESFFRQ
                     SLPILKAEKKPFYAFLITLSSHYPFNMDLDKIPELPLGELAGTQLGNYLRCIHYADAQ
                     IGAFVEGLRKEGLLDESVLAIYGDHPGLTVADRQDLARFLGVPDLSDPVAWKKAQKIP
                     FIVRLPRGRLRGQFSLAAGQIDIGPTLGNLMGTDLVTAFGQDLLNADRGFVVFRNGSF
                     LDDEAWVLPEQDLVYDLRTGQSRPLSGAPEGLARAREKLTLSDAILQENLIPKLNASF
                     Q"
     gene            10073..>11447
                     /gene="rmuC"
                     /locus_tag="PLY83_03275"
     CDS             10073..>11447
                     /gene="rmuC"
                     /locus_tag="PLY83_03275"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOV29093.1"
                     /translation="MSIPSGTAFLVGLILGGLGGALFLWPRLRQAGSELASLRVEAVR
                     LEERLKAQEEEQARLEAEAEEREAENDALRETVGRLQAERSAMEARMEEQAAAAESRL
                     ALLEEAKARLSEAFQALSREALEINSKTFLQLAGETLGQKSQAIDTVVQPLKESLNKV
                     EAAMREIETKREGAYGSLTEQIRGLSEAQLRLQGETANLVKALRQPQVRGRWGEIQLR
                     RVVELAGMIEHCDFEEQVSVTREEGRLRPDLIVSLPNNRIILVDSKVPLEAYMEAVEA
                     ADEAVRAEKLKSHSRQIREHISKLASKDYWKQFEQTPDFVVLFLPGESFMSAAAGTDL
                     ELIEYGAKNKVLLATPTTLLALLKAVAYGWKQEAVAQSARAISETGRELHDRLKAFLG
                     HFDGVRKGLKTASDSYNKAVGSLENRVLVTARRLAVLNHTDEEELGCPAPVEVPLRIP
                     SDLDEPEG"
     assembly_gap    11448..11547
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11792..13222
                     /locus_tag="PLY83_03280"
     CDS             11792..13222
                     /locus_tag="PLY83_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOV29094.1"
                     /translation="MDERVKTDVLVVGGGPGGRVSYMALKQFGVADVKMVANEEPTVI
                     CSLPYGIGRRLVPEGPEAVVVDLTRSHRLPPTILQDVLQGNCVELDLDRREAMVQGKG
                     GTKTVTFEHLVLAPGAVPWIPPVEGVLGGKGAETGTGILYGDEWVDKGRLAENVFVLR
                     GANDARRLDAFASKRGKAVVVGTGAIGLETAEALADRGVSVVLVEALPHAVAALDGDM
                     ALRLEERLAARNVKVFKSARVDRVLPEGLKLEDGTLLEADGIVFATGVRPDLRLAKQA
                     GLSIEKGIRVDSRMQTSHPRVFAVGDAVQVTDAATGAGILPLIGTLAMRQAMVAAANI
                     AGRAMELPPVTAWGVSEIFGLHWGSVGWTEEMATRAGIPVAALFQPVRTRDPFMPEGR
                     EGTWKLVVAAAQHGSVKPGQILGFQVVQEGDSPLFLAERFLDIIARRETVTDLFAHYF
                     IHSPAHNAVDDPYLGLLGQAMRMFGQ"
     gene            13385..14032
                     /locus_tag="PLY83_03285"
     CDS             13385..14032
                     /locus_tag="PLY83_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOV29095.1"
                     /translation="MSAEDRAYREIIQLILSGQFKPGDFLLEVDLAPRLDMSRTPVSR
                     ALGRLVAEGFLNKMPKKGCYIPFPSPEDAEQIFFARMAVEGQAAASAALKATDQEIRK
                     LKRILRTDQEAVAKGSKEIFAQINEQFHLGIARASRNVYLEKWVINSFWRSNIYIFYF
                     DGFYKAPVREEIPPQRTPSQHLAILKAIEERNPEEASFQMREHIRNTYELLLLGR"
     gene            14214..15455
                     /locus_tag="PLY83_03290"
     CDS             14214..15455
                     /locus_tag="PLY83_03290"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOV29096.1"
                     /translation="MAGKRVVQLGYGMQGKASLVDLVQAPGIDEIVVTDCYPGFEADI
                     AALGNPRVRAVKVDASRREELASVMAGADVVVELLPGLFALPVAQLAAELGVNLVSAM
                     YLANPGEQDPARREANFRELARIDAVMKEKGKTILEEFGMDPGIDLVLGRKCLDGLDE
                     VQAFHSYGAGFPELEAANNPIRYKFTWSVIGVMRSYLRPAVVIKEGRDVAVKADEMFS
                     PANTHILDLPEMGGPLECFPNGDSSHFARAFGIRETVKSMGRYICRWPGHAAFWGVMA
                     KSGFLSDDPIRVGDCEVAPTAFCAALFGSQKQFFYGEGERDVALVRTDARGLKDGKPW
                     RVICQVIDKRDLATGLTAMQRTVGFPVSIGAQMILDGRLDKKGIVSPSEVPFEEFMQE
                     LGRRGITWNREEGPWDGRMEP"
     gene            15431..16408
                     /locus_tag="PLY83_03295"
     CDS             15431..16408
                     /locus_tag="PLY83_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29097.1"
                     /translation="MGRPDGTLEIPFIKGHMGGNTIALLPGENLPEEGLLETVLLALR
                     DTCLGCHEAGLVYPSPLPGRLRLRIVGRSSRRFITACGGMTQVLGAAIGCGMLAEVMG
                     FGPCGSSRLVLETEAGSVPLSVSVSGASVRTETGMTPFLEEVRRSGIENIVLEGTRAV
                     RAGKFLVVRADDLLDRFEAGEVVGLTPRVRSALTEIQSQFLARYPQASLDFAVYDLRP
                     ERPGNLGRLVFPHWLPEGHVEPACGTGTIAVCAVLAFLGKLPGSHGGGCLEVRFESGG
                     GPDLGGPDITTAIMEFSAGEIESIRFSHSFVELTSRGKVMLSLKDRSPE"
     gene            16458..17876
                     /locus_tag="PLY83_03300"
     CDS             16458..17876
                     /locus_tag="PLY83_03300"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HOV29098.1"
                     /translation="MENIAAKGIISLVPIVLTLFLAFFTKDAVFSLVIGCIAGVLIAG
                     FDPATGLSKLFQHALGNGDFIWVLMIEVAIGIMIAFYLKAGVIGAFADWASTKIRTRR
                     QASGFGWVLGVLIFFSDYFSPLFSGPIARPLTDKYKVSREMLAYELDSGSAPVCTIIP
                     LSGWAVYIAGLLKGYGPITTAEEGMNAFVRSIPYNFYGWLAVILCGLIAFQLIPNFGP
                     MKKAEERALKEGKVIRDGATPLSGPEFDEISMVEGKKPNLMVYLFIPVLLVIGIALGT
                     FFILHSTKTLEAFFTAVMYQAVVLTIGGYFKDIKDAMDTAYKGIKAVLPAIMILALAY
                     CINDISKALGAQKYIFNMAQSWMTPAMLPLATFIAGALISFFTGTSWGTYAILTPFVL
                     PLAMQLSGNTLSPLVFATIGAVVGGGCFGDHCSPVSDTTCLSSFGAGSDHMDHVSTQI
                     PYAFTAAILAAVVYGVLGVMSV"
     gene            17952..18659
                     /locus_tag="PLY83_03305"
     CDS             17952..18659
                     /locus_tag="PLY83_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HOV29099.1"
                     /translation="MKTQKAEIGILCWEAGQVPRGLVQLESLVGNSTNPDSYAFPVRF
                     CRVKGANIHTILENPSREVLQTMIAEAKAMVEDGVRAITTSCGFNAIFQEELAEAVDV
                     PVFTSSLLQVPFAQKIVGRKSEVAILTAKAAALKPEHLAAAGISRTDNLHIHGLEGCP
                     QWSKIFTSPDEDMDLEAVRKEIVGVALDALRARPRTGAFVLECTDLPPFAEEIRNETG
                     LPVFDFITLVNYAYASL"
     gene            18694..>19126
                     /locus_tag="PLY83_03310"
     CDS             18694..>19126
                     /locus_tag="PLY83_03310"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HOV29100.1"
                     /translation="MKRVLVFGAGRVARPCVQYLLKQEGIEVTVVDAVPENLARVTEG
                     HPRSRSVVADAGKDSAGLIASEKPDLVINLLPPAFMAPVARQCLEAGVNLVHPAYLDD
                     ETLALDAPAKASGLVFLAELGLDPGIDHMSAARTVRRIREAG"
BASE COUNT         3530 a         5791 c         6110 g         3595 t
ORIGIN      
        1 ctggtgggtg tcgtggaaga cctggcccgg gccaggatcc ccgaggccat cccgcaggaa
       61 acggcccggc aggtccttga agggctcgat gcccgcattt cggggcgtta aggaaacgtc
      121 gatttaaaaa gaggcgagat gagatggatt cgcaaggctt ttttgctaaa taccggtttt
      181 ctatcttgct tttggccaca atggcgctga ttacggcctt tgtgctatcg agaccccagg
      241 aggttacaac aggggttcct gtcatgcaaa tctcaatgga ggccattttg attgtcctgg
      301 ggtttgctgc gggactgctg ggcgggtgga ttgggacagg cggatgcagc gtcatgcttc
      361 cggttttgca tttctggctt ggttttcctg cccccgtcgc cgttggcacg acgctgttcg
      421 ccgtcatttt tacggccata tccgggggat acgggcattt caagcaaaaa aacgtggaca
      481 agcataccgc gatatggacc ggcggagccg gaattgccgg agttttgctt ggatcctggc
      541 ttttcacttt cctttcgaat aaatccgccc ttctgggatg cctgatcggg ctcgttttcc
      601 tgtggccttc gttcaggatg atctgggaag ggtttcttgg tttcaggaat cgaaaattga
      661 cctcgggaat ggcaggaacc gcgcaggggc agccgataaa aaaagcgccc ccggtccaat
      721 tggcgtcatt tggggcggct gtcggaattc tcacggggat cgtggggcta ggcgggggct
      781 atgcgcttgt ccccgggctc atttacctgt ttgggatgcc tgtttatgtc acgatgggga
      841 cttccctggc gattatgatc cccatggcga ttgtcggggg agccatcaag ctttcccagg
      901 ggtatgtttt cctttccgca gcccttcttc tggctatggg aacgatcgtc ggggcgcaag
      961 tcggcgctgc cacgataaaa aggttcaagc cctatgccct gaagttgctc tttggccttt
     1021 acttcctcta cgtggcagtg aaattcatcg gcaacttcct gggcatcccc gtgtggtagc
     1081 aaaccgaaac ggccaacagg ggcgagggga gctctccagc cacgaaaacc ctgaccggaa
     1141 agccggagtt gccagggggg cgccaggtcc acgagggcca gggtcggccc ccctgcaatc
     1201 atgccacttt ggggcgggtt tgggccgcaa ctgggcatgg aaacccaacc ccgcaagcct
     1261 atgatggaca aaaaggctga aacgccgaag aaggaggcat gcaagatgag aatcctgaag
     1321 gcaggaagat ggggtgcggt tgcggcgggg atcctgtcgc ttctgttcgc aggggtggcc
     1381 tttgcgtccc agcccgtcct gaacgcgtac acgatctggt ccgaaaggta cgcgaacgag
     1441 attttcaccg cgttcacgaa ggataccggc atcaaggtca actggatgcg cttttcctcc
     1501 ggggaactcc aggcgaggct ggaggcggag aaggcaaacc cccaggtgga cgtggtcttc
     1561 ggcgacctgg ccgaggcctt cgcgagcggg atcccgaaag gcctgttcga gccctaccgg
     1621 cccgccggca gcgaggccat cccttccagg ttcaaggatc cgaaggggta ctggacgggc
     1681 gttgcacttg accccatctg cttcatgttc aactcgaaat tcctcaagga acatggattg
     1741 aagccgcctg cttcgtggca ggacctgctg gacccggcct accgggggaa gctgcagatg
     1801 gcggacgccc ggacttccgg cacggcgatg taccggatcc tcagcctcgt ccaggccatg
     1861 ggggaaaacg aggccttcgc ctaccagaaa aagttgaatg ccaacgttca aatctacacc
     1921 aagagcggcg cgggcggtgc cctgccgatc gccagggggc aggccgcggg agggatcttc
     1981 ttcctcgtgg acgccctgga ggtccagcag acggggtatg acgtgacgat cagctacccc
     2041 agggaaggcg tggtcgcggg aatcgaggcc atggccctgg tgaagggggc ccggcagccc
     2101 gaactggcga aaaagttcct ggactgggcc tccaccgagc gcatgcagcg aatctacgaa
     2161 gcgagccgga tcaacctgat cccgagcaat ccaagggtga aggtctcgag ccccgggctg
     2221 gacctctcga aggtgaagat cctgccgctg gacctggagt gggcggggaa aaaccgggaa
     2281 cgcctcgtcg aacgctgggt gaacgaagtc ctctaagaag gggaatttcc ggtggcggcg
     2341 ggtttcgggc aaaccgtgcg gagtggaagg gccaggctcg atcctttgac cctggctctt
     2401 ctgctcgtcc tgtgggcagg gttggcggtg ttcgtgctct acccgctggc gcgcctgctc
     2461 gcctccgttt tcgctgccga cgggagcgct tccctggaga acctggccaa ggtgttccgg
     2521 aacgccacca cccggaaagc gctggtcaac agcctggtcc tcgcgggagc cgtcgcggtg
     2581 tccgggacgt tttgggggta cctgttcgcc ttctccgtca cccggatccc cctgccgaag
     2641 gccttccggt ggttcctgaa aggagtggtg gtccttccgt tcgtgtcccc gcccttcacc
     2701 agcagcattg ccctcaccct ggccctgggg ccgggggggc tgctgctcag gcttttccga
     2761 gtcccggact tcaacctcta cggttttgcc ggaacctggg ttgccgagac gctggcgtac
     2821 ttccccatcg cgtttctggc cctttcggcg atcctggaag cgatcgaccc caacctggag
     2881 gaggccgccg cgagcctggg cggttcccgc tggagggttt tcaggaccgt gaccctgccc
     2941 ctgagcgtcc ccggcatcgc caacgccttc ctgctcctct tcggcgcgtc cctgacggac
     3001 tttgccacgc ccctcgtgat ggccgggcac acttttcccg tcctgacgac ccaggcatac
     3061 ctccagatca ccgggatgta cgaccttcgg ggaggcgcgg ccatctcttt cgtcctgctg
     3121 ctgccggctc ttctggtcta tctcctccag cagtcctggg tggagcggaa aagccaggtc
     3181 acggtgacgg gcagggtcgg gcctcgttcg gctccccggg gggcgggacg catcgccggg
     3241 atcctggtgc tttcggccgt ttcggtcctg acgctgttca tcctgttttt gtattcgatc
     3301 attttcctgg gatcgttcgt gaaggtctgg ggcgtggacc acggcctgac gctcaaaaat
     3361 tacgcgtacg ttttcacggt cgggtggaaa gccgtccggg acacggtcct catcgcggtt
     3421 gccgccacgg tcctgggaac ggtcctggcg gcattgaccg ggtatttgtc cgctcgaagg
     3481 gaagtgccct tccggaaagc gctgggattc gtttccctcc tgaactacat ccttcccgga
     3541 acggtcgtgg ggattgccta cgtgatcgcc ttcaactcgg gtcccctggt gctgaccgga
     3601 acgatgacga tcctcgtcgc cctgtgcgtt ttccggtacg atgccgtggg aatccggtcc
     3661 acggaggccg ctctccggca ggtcgaccct tccctcgagg aggcgtccta cagcctcggg
     3721 gccggtcggg ccgccacctt cttccgggtc acgcttcccc tcgtcgcccc ggccgtcgcg
     3781 gcgggggccc gctacctctt cgtggtctcc atgacggcga tcagcgccac gatctttttg
     3841 gtctcggtcc gctggagcct gctgacggtg aggatcctgg agtgcgtgac cgagctgctt
     3901 ttcgcagagg cggcagcctt ttcggtggtc ctcatcctca tcgtgtttct ggcttccggt
     3961 gcgatcctgc ttctgcttcg acttgctgtc cctcaatctc ccggcggctt gaggaggtcg
     4021 taatcgatgt ccctcggtat ctcgcttcac cagctgagca aacgattcgc ccggggaaag
     4081 gaggccgtgc cggcggtgga cggcgtcagc ctcgacgtcg aggcgaagga gatggtggct
     4141 ttccttggac cgtcggggag cggcaagacc acgatcctga ggatcatcgc cgggttcgag
     4201 aggccaacgg agggtcgggt cctgatcggg gggcaggatg tcacggacct ggaagcgaac
     4261 gccaggggga tcggcttcat tttccagaat tacgctctct tcccgcacat gaccgtgttc
     4321 gacaacgtcg cctacgggct cagggcaaag cgcgtccccg cgggggagat tccccggaaa
     4381 gtggccgagg ttctcgagat ggtgggcctg aaaggcgtgg aaaaacggtt ccccaaccag
     4441 ctttcgggcg gggagcagca gcgggtggcc ctggcccgcg tgtttgtcct ggacccccgg
     4501 gtgctgctga tggacgaacc tctcgccaac ctggacgcaa gcctccggac gcgcatgctg
     4561 gccgaaatcc gcaggctgca aaggcacctg ggcacgacct gcatctacgt aacgcacgac
     4621 cagaaggagg ccctgaccct ggccgaccgg gtggcggtcc tgaaccgggg aaggctggaa
     4681 caggtcggga cccccctgga gctttacgaa gccccgcaga acctgtttgt tgcggatttc
     4741 atcggccagg cgaacctcct gcggggcgtc gtgaaggccg tttccgcgga ttctgccgaa
     4801 atttccgtgt gcggccggag cctgcgggtc cgccgcggaa ggatcgaaac gctgaaaagc
     4861 ggggactcgg cggggatcgc ggtcagaccc tccgccgtga gcctgggggc cgcgggggag
     4921 gcgttccttc agggcagggt ggaaagccga atcttcgccg gcgaccggat ggaatacgaa
     4981 atcgaggtgg aggaaggggg catggttcgc gcttacgggg ccctgccggg aaggggagtc
     5041 gtcttttccg agggggatcc tgtggggatc catttcgatc ctggcgaaac catggccttc
     5101 cccctgtaac aaaacggccc cccgccgggg cggggagcca acgtgttctt tagaggcgtt
     5161 cgtctccgct gtgccacttc tggatgggcc ggtagtcgat gggaacggaa aggacgatgg
     5221 aagtgacggg ctgcccgaac ttggataggg agtccagcag cgcttccagg tgggacacgt
     5281 tcgtcacgat gaccttgagg agataggaat cggcccccgt gacgcgctcg cacgtaagga
     5341 cttccttgcg ttcccggagg aaatcgagga gctcttcctg ccgctcccgg gaagagctga
     5401 gccggatggc agcggtgatg gggtagccca tcttctcggg atccacgatc gccctgtatc
     5461 ccttgatgat cccggcttct tccatgcgcc ggacccgctc cgagatcgcg ggcgaagaaa
     5521 ggcccacccg cttgcccagc tcgctgaagg tgatgcgccc gtcttcctgg agggccgaca
     5581 ggatttccaa accaatgcga tccagcaatc tttctgcttc gaggttcatc atgaacaccc
     5641 tttctatgtt ttaaagaaat taggttttct tgcagccacg caatcgtatc aaaaagcgaa
     5701 gtccctgtct agaacaaaaa aagtgttttt tagggtcgat ccttgataga ggggcgatct
     5761 gtgaaaaaaa gaacagccct tccaaaaaaa agccaggttc gaagtccgtt aatcggtcgt
     5821 tcctgcccgg gcccgccttt tcagcagcac ataggccccc agcacgagca ggcagaagcc
     5881 cgcaaggagg atcgtgatcc ggtgaagggc cgcggtcatt cccgtttcgt ctcccccgag
     5941 ccagaatccc acgagagcca ggacgaccac ccagattccc gagccgaggc cggtgaagaa
     6001 cagaaagagc ggaatgttca tccgggccag tcccgcgggg agggagatgt actggcggat
     6061 gcccggaagc aggcgtccca cgaacgtgct gatgtgcccg tggctggcga agaagcggtc
     6121 ggccttctcg atggatttct cactcaccag gaaatagcgg ccgtaccgga ggaagaacgg
     6181 acggccgaag cgaagggaga tccagtagtt gaacagcgct cccagcaggc ttccgaggat
     6241 gcccgaggcg atcaccaggg aaaggtgcat ctggcccctc gcggcgaggt agcccgccgg
     6301 agggaccacg acttcgctcg gaaaggggaa gaacgaggac tccaggaaca tcagggccac
     6361 gatacccgga tagcccatgt gcccgatggt attcacgagg aaggaaacga aagcagcaag
     6421 caaggcgctt ctcccgcgga agccgccgct aggcggagcg cctggcggaa agctgctcga
     6481 tggaaatcct tcgggtgtgc tcggtcttga cccagacggc ctggctgctt gccatgaagg
     6541 cggcccggaa gaggatgggg atccacgtca ggccgaagag gaagtaggcc ggaaccgcgg
     6601 agacgtattt caggacgagg cgtccgctcc gcttcgaggg tccgacaacg tcgacccagg
     6661 cgcagtaggc aaggatcatc aaaaacggca ccgagatgaa gaacgcccat tccaatgccg
     6721 tctcggggac cttcgcccca agcgtcggga agaggatggc gttgttcacc agggtgtaga
     6781 tcatcccggc gatcatgacg atcagggaga cgcaggcctt gacgggggcc atcaggtaga
     6841 gggccaggtc caggtactgg agcctccggg tctgggcgaa gcgcttgagc gccgcgaaga
     6901 cgtagtgcca gcagacccag taatgtccct gcatccatcg agtccgctga cgccaggaga
     6961 ccttgaggct ctggggcttt tcgtcgaaaa ggcgggcatg gtcgttccag tgaactcgct
     7021 tccctgaaag aatgaggcgc gtggaaagct ccaggtcctc cgtgaggctc tgcatgttcc
     7081 accccaagcg ccgcatggtg gcgcttttga ccacgagccc cgtccctccc agcgtgcagg
     7141 aaagccccca ctttgaacgg gccagctgcc agaagcggtt cgtgtaccag taggccagcg
     7201 cgtaggcccg ggtgagccag ttgtcgtccg ggttcttcac gtccaggcag ccctggatcg
     7261 cctccgcgtc cgggtgggct tccatgtagt cattcatgcg tgccaggaaa tccggggccg
     7321 ccaggttgtc cgcgtcgaac atgaccaggc cgtcgtaatc ctcgaggcgg attcgctgca
     7381 gggcccagcg gacgttccag gtcttcccgt tcttcgtctt gtcttcccgg atgagggcga
     7441 tggcgccatg gcttttggcg acgtccgccg tgttgtccga gcagttgtcg caggagacat
     7501 acacgtcgaa ccgttccctc gggtattcct gcaggttcag gctttcgagc aggccgtcga
     7561 gaactgccgc ttcgttgtgg gcgggaatga gaatggcgaa ccggcggaaa gtcttcgccg
     7621 cgggaagggg cctggcgctc caccacccgc ggaaaccgat cacgaactgg tacgcggcat
     7681 ccaggaagag aaaggcgaaa agggtgtaca agccagcctg gagcagccac cagccccacc
     7741 ataaggtcca gaaaaacagt tcattctgct gggcttccat ctttgaaagg ctcccttttg
     7801 aaggttgagg ttcagcgtgt gtcatcatag tgattcaggc aaaaaaacgc aagaggtcct
     7861 tttaaatttc gcaacttgcc tttgatgtgt gcgatttttc ctagaaaaaa gttaagactc
     7921 ttaattttga tcgatccgta acgctacggt ttccattttg atccacaggg tgtaaaatgc
     7981 ccgcgagaaa aaatcgactg tcctttcctg acattccctg cttatttcta ttgatataaa
     8041 gaggaaggaa agtcgcccgg aggaatgcac gtggctcgga tcgaatccct gaaacagcgt
     8101 ttcgacagca acgaactggc cctgaccggc tttctggccc tggccgtcag cctgaaattc
     8161 gtgggcctca acaataccct gatgagggga tccctggaag acccgctgac gcttctggcc
     8221 ctgatgggca cgtccctcgc cctggcgagc ctgcctctcc ttctgccccg ccgtttccgc
     8281 ggggcaggaa tgcttttcgt cgacttctgg ctcacggtcc tggtttggac ggatctgctt
     8341 tctctccgtt actattcgga cctcttcacg ctccggaacc tcggccttgg aacccagctc
     8401 tccgccgttg cggattcggt ggtggccctg atggagccga aggacgccct actctggatc
     8461 gacttcccgg tcctggggta cctggccttc cgcgtcacgc ggcgtttccg gagtcttcct
     8521 gccctggact ggggtcgggc aggagccctt cttctgctga cggcgatcct cctggttccc
     8581 ttcgggctga gggtccagcg gttcgagaag acctgtccgg gagcccttgc ttccatgtgg
     8641 gaccgtcctg cggtggccct cagcctgggc tccctgaatt accacctggc cgacctgctt
     8701 tccgtcgcga cagaaaggtt ccgcgtgcgc aaaccgggca ccgacctgga agaagtggca
     8761 ggctggtttc aggagcgtcc cggggcgggg gtccagcgct accacggcat cgcttccggc
     8821 aagaacctga tcgtcatcca ggtggaagcc cttcaggcct tcgtcctgaa ccggtcggtc
     8881 gggggcgtcg agatcactcc gaacctgaac cgcctggccc gccgaggcct caatttcacg
     8941 gaaacgtacc accagaccgc catgggcaac agcgccgacg cggagttcct ggtgaacacg
     9001 ggtctcttcc cggctcccag cggcgtggcc tacacccgct acgcgggaaa cgagttcaac
     9061 tcgatcgcct cggccctgaa ggcgaaaggc tattccacga tcgccctgca cggggatcgg
     9121 ccgggcttct ggaaccgtgc ccggatgtat ccctccctct cctttgaccg ctttgtcagc
     9181 aaccgggatt tccaggtcga ttcgatcatt ggcctggggc tcagcgacga gagcttcttc
     9241 cgtcaaagcc tgcccatcct caaggccgag aagaagccct tctacgcctt cctgatcacc
     9301 ctgtccagcc attacccctt caacatggac ctggacaaga tcccggagct tcccctggga
     9361 gagcttgccg gcacgcaact gggcaattac ctccgctgca tccactacgc cgacgcgcag
     9421 atcggggctt tcgttgaagg actgcgaaaa gagggactcc ttgacgagag cgttctggcg
     9481 atctacgggg atcatccggg gctgacggtg gcagaccggc aggacctggc ccgcttcctc
     9541 ggggtgcccg acctttccga cccggtggcc tggaagaagg cccagaagat ccccttcatc
     9601 gtccgcctgc cccgggggag gctgcgcggc caattctctc ttgcggcagg gcagatcgac
     9661 atcggtccga ccctggggaa cctgatggga acggatctcg tcaccgcctt cgggcaggac
     9721 ctcctgaacg ccgatcgcgg ctttgtcgtc ttccgcaacg gctctttcct ggacgacgag
     9781 gcctgggtcc ttccggagca ggacctggtt tacgacctgc ggacggggca gagccgcccg
     9841 ctttccgggg ctcccgaagg actggcccgg gccagggaga aattgaccct ttccgatgcc
     9901 atcctccagg agaacctgat ccccaagcta aacgcctcct tccagtaggc acttcgaagg
     9961 ccggcacctt ccgcccgggg gtgccggcct tttttcgtgc cccccgtttt tccccgaccc
    10021 cctataatcg ggatgagaca cgatcctatc ctttgcggga ggcgatgaag ggatgtcgat
    10081 cccaagtggc accgcgttcc ttgtggggct gatcctggga gggctgggcg gagccttgtt
    10141 tctgtggccc cgcctgcggc aggccggcag cgaactggct tctttgaggg tcgaggccgt
    10201 tcggctggaa gagcgcctca aggcccagga agaggaacag gcccgtctcg aggcggaagc
    10261 ggaagaacgg gaggctgaaa acgacgccct ccgcgaaacc gtcggaagac tgcaggccga
    10321 gcggtcggca atggaagccc gcatggagga acaggccgcg gcggccgaat cccgccttgc
    10381 cctcctggaa gaggcgaaag ccaggctttc ggaggccttc caggcccttt cgagggaagc
    10441 gctggagatc aacagcaaga ccttcctgca gctggccggg gagaccttgg gacagaagag
    10501 ccaggcgatc gacacggtgg ttcagcccct gaaagagtct ctcaacaagg tcgaagcggc
    10561 catgcgggaa atcgagacga aacgggaagg ggcgtacggg agcctgacgg agcaaatccg
    10621 ggggttgtcg gaagcacagc tccggctgca gggagagacg gccaacctcg taaaggccct
    10681 gcggcagccc caggttcgcg gcaggtgggg ggagatccag cttcggcgtg tggtcgagct
    10741 ggcggggatg atcgagcact gcgacttcga ggaacaggtc tcggtcaccc gggaagaggg
    10801 gcgtctccgg ccggacctca tcgtttccct cccgaacaac cggatcatcc tcgtggattc
    10861 caaggtcccg ctggaggcct acatggaggc cgtcgaggcc gcggacgagg cggtccgggc
    10921 ggaaaagctg aaaagccaca gccgccagat ccgggaacac atctcgaaac tcgcctcaaa
    10981 ggactactgg aaacagttcg aacaaacgcc cgatttcgtc gtccttttcc tgccggggga
    11041 atccttcatg agcgcggcgg ccggaaccga cctcgagctg atcgagtacg gggcgaaaaa
    11101 caaggtgctg ctggccaccc cgacgaccct gcttgccctg ctgaaggccg tggcctacgg
    11161 ctggaagcag gaagcggttg cccaaagcgc ccgtgccatc agcgaaacgg ggcgggaact
    11221 gcacgaccgg ctcaaggcgt tcctcgggca tttcgatgga gtccgcaaag ggctgaaaac
    11281 cgccagcgac tcctacaaca aggccgtcgg ttcgctcgaa aaccgagtcc tggtgaccgc
    11341 caggcgcctt gcggtcctga accacaccga cgaagaggag cttggctgcc ccgcgccggt
    11401 ggaggttccc cttcggatcc cgtccgacct cgacgagccg gagggagnnn nnnnnnnnnn
    11461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11521 nnnnnnnnnn nnnnnnnnnn nnnnnnngcg ccggtggagg ttccccttcg gatcccgtcc
    11581 gacctcgacg agccggaggg agagaaggaa gcctcctcca gccagcctgg ggcgcaatcc
    11641 agcctctaga tcgaccggtt tttgggaaaa gcccgctgcc ctgaaagccc tgcccgctgc
    11701 agcaggcagg gcaaaaggtt caacccgctt gacaaatacc ccctataggt atagcatgtg
    11761 gccgatttct caaaaagggg gaacaatcca gatggatgaa cgcgtaaaga cggatgtcct
    11821 ggtggtcggg ggcggacccg gcgggcgggt ttcgtacatg gctttgaagc agttcggagt
    11881 ggcggacgtg aaaatggtgg cgaacgaaga gcccaccgtg atctgcagcc ttccgtacgg
    11941 catcgggcga aggctggttc ccgaaggacc ggaggcggtg gtcgtggacc tcaccaggtc
    12001 tcaccgcctg ccgccgacca tcctccagga tgtgctgcag ggcaattgcg tcgagctcga
    12061 cctggaccgc cgcgaggcca tggtgcaggg aaaaggcgga acaaagaccg tgacgttcga
    12121 gcatctcgtc ctggcccctg gagcggttcc ctggattccc ccggtcgagg gggtcctggg
    12181 aggcaaaggg gccgaaaccg gcaccgggat cctgtacggc gatgaatggg tggacaaggg
    12241 ccgcctcgcg gagaacgttt tcgtcctccg cggcgcgaac gacgcccggc gcctggacgc
    12301 ctttgcgtca aagcggggaa aggcggtggt cgtcggaacc ggggccatcg ggctggaaac
    12361 ggcggaggcc ctcgcggacc ggggagtctc cgtggtcctc gtcgaagccc tgccccatgc
    12421 cgttgctgcc ctcgacgggg acatggccct tcgcctcgag gaaagactgg ctgcccggaa
    12481 cgtgaaggtc ttcaagagcg cccgggtcga ccgggtcctt ccggaaggat tgaagctgga
    12541 agacgggacg cttctcgagg cagacgggat cgtcttcgcc acgggggttc gcccggacct
    12601 ccgcctcgcg aagcaggcgg gtctttccat cgaaaagggg atccgtgtcg atagccgcat
    12661 gcaaacctct cacccccgcg tgttcgcagt cggggatgcc gtccaggtaa cggatgcggc
    12721 cacaggcgcg ggcatcctcc ccctgatcgg tacgctggcc atgcggcagg ccatggtggc
    12781 ggcggcgaac atcgccggcc gggcaatgga gctgcccccg gtcacggcct ggggcgtcag
    12841 cgagatcttc ggtcttcact gggggagcgt cggctggacg gaagaaatgg caacccgggc
    12901 agggattccc gtggctgccc tattccagcc ggtccggacc cgggatccct tcatgcccga
    12961 aggccgggag gggacatgga aactggtcgt ggccgcggcc cagcacggga gcgtcaagcc
    13021 cggccagatc ctcggctttc aggtcgtgca ggagggcgat tcgcccctct tcctggcgga
    13081 gcgctttctt gacatcattg cccgccggga gaccgtcacg gatcttttcg cgcactactt
    13141 catccattcc ccggcacaca acgccgtgga cgacccctac ctcgggctgc tcggccaggc
    13201 gatgcggatg ttcggccaat aaggcttccg ccacgtctct tccttttaga gcaaggacgg
    13261 atcaacgggg ggccctgtaa ggggcacccc cgtttcgctt gacgggtcgg ggccgggatc
    13321 ctacaatcaa gccttggatg catcattcat ggatacatca acggggggat tcccagtgga
    13381 aaaaatgagc gcggaagatc gggcctaccg agagatcatc cagctgatcc tttccggtca
    13441 gttcaagccc ggagacttcc tgctggaggt ggacctcgcc ccgaggctcg acatgagccg
    13501 gacacccgtc agcagggcgc tggggcgcct cgtggccgag ggattcctga acaagatgcc
    13561 caagaagggt tgctacatcc cgtttccgag cccggaggac gccgaacaga tctttttcgc
    13621 ccgcatggcc gtggaggggc aggccgccgc ttcggcggcc ctgaaggcta ccgaccagga
    13681 aatccggaag ctcaagcgga tcctcaggac ggaccaggag gcggtcgcca aagggtcgaa
    13741 ggagatcttc gcccagatca acgagcagtt tcacctgggc atcgccaggg cctcgcggaa
    13801 cgtgtacctc gagaagtggg tcatcaacag tttctggcga tccaacatct acattttcta
    13861 tttcgacggt ttttacaagg cccccgtccg ggaggaaatt cctccccagc ggacgccttc
    13921 ccagcactta gccatcctga aggccatcga ggagagaaat cccgaagagg cgtctttcca
    13981 gatgcgggag cacatccgta acacgtacga actcctgctt ctcggaagat aatcgaaaac
    14041 aaatccaggg aacttcttgc aaggggaagc cgaatcgcgg cttccccttg ttttattgtt
    14101 acgcatttca cgtttggaac aggattgaca gaagagcggc caggtgatat gcatttatca
    14161 agaatccaaa aaatattcat agatgaatac ataaatccgg ggaggcgact ttcgtggcag
    14221 ggaaaagggt tgttcaactg ggatacggga tgcagggaaa ggccagcctg gttgacctgg
    14281 tgcaggcgcc gggcatcgat gagatcgtcg tgacggactg ctacccgggt ttcgaagcgg
    14341 acatcgcggc cctgggaaac ccgagggtcc gggcggtgaa agtggacgcc tcgaggcggg
    14401 aagagctggc ttccgtcatg gcgggtgcag acgtggtcgt cgagctgctt cccggcctgt
    14461 tcgcgttgcc cgtggcgcag ctggccgccg aactgggggt caacctcgtg tcggccatgt
    14521 acctggccaa ccccggtgaa caggatccag ccaggcggga agccaacttc cgggaactgg
    14581 cccggatcga cgccgtgatg aaggaaaagg ggaagacgat cctggaggaa ttcgggatgg
    14641 atcccggcat cgacctggtg ctgggccgca agtgcctgga cgggctggac gaggtccagg
    14701 ccttccactc ctacggggcc ggctttcctg agctggaggc ggcgaacaac ccgatccggt
    14761 acaagttcac ctggtccgtc atcggcgtga tgcgctccta cctgcgcccg gcggtcgtca
    14821 tcaaggaagg cagggacgtc gcggtcaagg cggacgagat gttcagtccc gccaacaccc
    14881 acatcctcga cctccccgag atgggaggcc ccctggaatg cttccccaac ggcgacagct
    14941 ctcatttcgc cagggccttc ggcatccgtg aaacggtgaa aagcatgggc cgctacatct
    15001 gccggtggcc cgggcacgcg gccttctggg gggtcatggc caaatcgggg ttcctctcgg
    15061 acgacccgat ccgggtgggc gattgcgaag tggctcccac ggcgttctgc gcggccctgt
    15121 tcggctcgca gaaacagttc ttctatggcg agggagagcg ggatgtcgcc ctggtgcgca
    15181 ccgatgccag gggcctgaag gacgggaagc cctggagggt gatctgccag gtcatcgaca
    15241 agcgggatct cgcaacggga ctgaccgcca tgcagcggac cgtcggtttc ccggtttcca
    15301 tcggagccca gatgatcctg gacggacgcc tggacaagaa aggaattgtc agccccagcg
    15361 aggtcccctt cgaggaattc atgcaggagc tgggacgtcg gggcatcacc tggaatcgcg
    15421 aggaaggccc ttgggacggc cggatggaac cctagagatc cccttcatca aggggcacat
    15481 ggggggcaac acgatcgccc tgcttcccgg ggagaacctt ccggaggagg ggctcctgga
    15541 aacggtcctc cttgccctgc gggacacctg cctggggtgc cacgaggcgg ggctggtcta
    15601 tccctcgccc cttcccggca ggctccggct tcggatcgtg ggccgttctt cccgcaggtt
    15661 catcaccgcc tgcgggggaa tgactcaggt gctcggggcc gcgatcgggt gcgggatgct
    15721 ggcggaagtc atggggtttg ggccctgcgg gtcctcgcgt ctcgtgctcg agacagaggc
    15781 gggaagcgtt cccctctcgg tctcggtgag cggggcttcg gttcggaccg agaccggcat
    15841 gacccccttc ctggaggaag tccggcggag cgggatcgag aacatcgtcc tggagggaac
    15901 gagggccgtg cgggccggga agttcctggt ggtccgcgcc gatgacctcc tcgaccgctt
    15961 cgaggcaggc gaggttgtcg ggctcacccc tcgcgtcagg agcgccctta cggaaatcca
    16021 gtcgcaattc ctggcgcgtt acccccaggc gagcctggac ttcgcggttt acgatctccg
    16081 cccggagcgt ccggggaatc tcggccgcct ggtttttccc cattggcttc ccgaggggca
    16141 cgtggagccc gcctgtggga ccgggaccat tgccgtctgc gccgtcctgg ctttcctggg
    16201 caaactcccc ggcagtcacg gaggcggctg cctggaggtg cggttcgagt ccggcggcgg
    16261 tccggacctg ggcgggccgg atatcacgac ggcaatcatg gaattttccg caggggaaat
    16321 cgaatcgatc cgcttttcgc acagcttcgt cgaattgaca tcgagaggga aggtgatgct
    16381 gtcgcttaaa gatcgatccc cggaataaga gaaaccgcaa ggaaacgaag cgaagccaaa
    16441 gaaaggagtg agtgcaagtg gagaatatcg ctgcaaaggg aatcatctct ctcgtgccca
    16501 tcgtcctgac gcttttcctg gccttcttca ccaaggatgc cgtcttctcg ctcgtcatcg
    16561 gctgcatcgc gggggtcctg atcgcgggtt tcgacccggc cacggggctg tcgaagcttt
    16621 tccagcacgc cctggggaac ggggacttca tctgggtcct gatgatcgag gtcgccatcg
    16681 gaatcatgat cgccttctac ctcaaagcgg gcgtgatcgg tgccttcgcg gactgggcct
    16741 cgacgaagat ccgaacccgc cggcaggctt ccggtttcgg ctgggtcctg ggcgtcctca
    16801 tcttcttcag cgactacttt tcccccctct tcagcggtcc catcgcccgt cccctgacgg
    16861 acaagtacaa ggtgtcccgc gaaatgctgg cctacgagct tgactcgggg agcgcaccgg
    16921 tctgcacgat cattcccctg agcggctggg ccgtctacat cgccggcctc ctcaagggct
    16981 acggccccat caccaccgcg gaggagggca tgaacgcctt cgtccgttcc atcccctaca
    17041 acttctacgg ctggctcgcc gtcatcctgt gcggcctgat cgctttccag ctcatcccga
    17101 acttcggtcc catgaagaag gcggaggaac gcgccctgaa ggagggcaag gtcatccggg
    17161 acggcgctac gccgctcagc ggtccagagt tcgacgagat ctccatggta gaggggaaga
    17221 agcccaacct catggtttac ctcttcatcc cggtactttt ggtcatcggg atcgccctcg
    17281 ggactttctt catcctgcac agcacgaaga cgctcgaggc cttcttcacg gccgtcatgt
    17341 accaggcggt cgtgctcacg atcggcggct acttcaagga catcaaggat gccatggaca
    17401 cggcctacaa gggcatcaag gcggttctgc cggccatcat gatccttgcg ctggcctact
    17461 gcatcaacga catctccaag gccctgggag cccagaagta catcttcaac atggcccagt
    17521 cctggatgac gccggccatg ctgccgctgg ccaccttcat cgcgggggcc ctgatctcct
    17581 tcttcaccgg cacctcctgg gggacctacg ccatcctgac gcccttcgtg ctgccgctgg
    17641 ccatgcagct ttccggcaat accctctccc cgctggtgtt tgccaccatc ggggccgtcg
    17701 tgggaggcgg ctgcttcggg gaccactgct ccccggtgtc cgacacgacc tgcctctctt
    17761 ccttcggtgc cggttccgac cacatggacc atgtctccac ccagatcccc tacgccttta
    17821 ctgcggcgat ccttgcggcc gtggtctacg gcgtgctggg agtcatgtcc gtctaggcga
    17881 aacagttcga tgaaagcggg ggtgcccggt ttttcgggcc ccccgcagat cttcttgcag
    17941 gaggcaagcc catgaaaacg caaaaagccg aaatcggcat tctttgctgg gaagccggcc
    18001 aggtcccgag gggcctcgtc cagctggagt cgctcgtcgg aaacagcacc aaccctgaca
    18061 gttacgcctt ccctgtccgc ttctgccggg tgaagggtgc caatatccac accatcctgg
    18121 agaaccccag ccgcgaggtc ctgcaaacca tgatcgccga ggcgaaagcc atggtggagg
    18181 acggcgttcg ggccatcacc accagctgcg ggttcaacgc cattttccag gaggagctgg
    18241 cggaagcggt ggatgtcccg gttttcacct cgagcctgct ccaggttccc tttgcccaga
    18301 agatcgtggg gcgcaaatcg gaggtcgcga tcctgaccgc caaggcggcg gccctgaagc
    18361 ccgaacacct tgcggcggcc gggatcagtc gcaccgacaa cctgcatatc cacggcctgg
    18421 aagggtgtcc ccagtggagc aagatcttca cttcgcccga cgaggacatg gacctggagg
    18481 cggtgaggaa ggaaatcgtg ggagtcgccc tcgacgcgct aagggcccgt ccgagaaccg
    18541 gggcgttcgt cctggaatgc acggacctgc cgccctttgc cgaggagatc cggaacgaga
    18601 ccgggttgcc ggtcttcgat ttcatcacgc ttgtcaacta tgcctatgcc tcgctgtagg
    18661 cggggcgggc gaaaaaacgg aggtctttct tcaatgaagc gcgttctcgt tttcggcgca
    18721 gggagggtcg cacgcccctg cgttcagtat ctcctgaaac aggagggcat cgaagtgacg
    18781 gtcgtggacg cggtcccgga aaacctggcg agggtcaccg agggccaccc gcggagccgg
    18841 tccgtcgtcg ccgacgcggg gaaagattca gccgggctga tcgcctcgga aaaaccggac
    18901 ctggtgatca atctccttcc ccctgccttt atggcgccgg tggccaggca gtgccttgag
    18961 gccggggtga acctggtcca tccggcctac ctggatgacg aaaccctggc gctggacgcc
    19021 ccggcaaagg cctcgggcct tgtcttcctg gcggaactgg gactggaccc cgggatcgac
    19081 cacatgtcgg cggcgcggac ggtccggcgc atccgggagg cgggcg
//
LOCUS       DAOOVN010000020        18667 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_2026_length_18667_cov_15.158178, whole genome shotgun
            sequence.
ACCESSION   DAOOVN010000020 DAOOVN010000000
VERSION     DAOOVN010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 18667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18667
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2026_length_18667_cov_15.158178"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..474)
                     /locus_tag="PLY83_03315"
     CDS             complement(115..474)
                     /locus_tag="PLY83_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29101.1"
                     /translation="MENERTAHPEEIADLCARVAARLIDSITRTAQIASSATPEMQEL
                     TRQWIELIGKEILNRVSTGEEIAVEELSRSIGITPSSLLSILLALHRGGRTSITHLRC
                     SPGSGRNEDLCHCMVED"
     gene            600..1643
                     /locus_tag="PLY83_03320"
     CDS             600..1643
                     /locus_tag="PLY83_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29102.1"
                     /translation="MAIRVLLLGFGNVGRNFCRLLEKQDPLLGNEEQVLLAGLATRGR
                     GTLWSGDGIPVSRLLEVERQTGRVDALSLPGCKKGLSAMDLVREGEFDILAECTSGNL
                     KAMESEPGFSHIRAALERKKPVATTNKAVAALRFEEVEALSRQAGVPWHFDGTVVVGA
                     PFLDYARRSIVDARILGFEGTLSATCNYVLDCLMGGASLDQALMDAQRSGMAESDPAL
                     DIDGWDSAAKLMIIAKALMGTPCPALGEFPVKGIRGLTRAVLAEARRSGGKIRLLARI
                     TRSAGGVDLSVGPEVLPPSHPLSLLFGPTNGAVILTENLGEICLSGTGSSPAQAAFAL
                     WQDIRRIARREKR"
     gene            1643..2188
                     /locus_tag="PLY83_03325"
     CDS             1643..2188
                     /locus_tag="PLY83_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HOV29103.1"
                     /translation="MAGRLGIIADTHGDLLGWESAVRAWGPIDLILHAGDVLKPWKSR
                     PLSHVGGKRALAEAINACDVPVLIARGNCDSEEDAQMVKWPMVEPFLFLWWNGRLLVA
                     GHGTDFSEIRERGIASEADVVITGHTHVASLVCERKTWFLNPGSASLPMGRDPASVAL
                     LDGEGIRILTLGNELLHFEPW"
     gene            2209..3036
                     /locus_tag="PLY83_03330"
     CDS             2209..3036
                     /locus_tag="PLY83_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOV29104.1"
                     /translation="MILIDLHTHSTCSDGTLSPEALVRAAKARKVSVLSLTDHDTTEG
                     VAKFLSACAHAGIRGLSGIELSAEAPYTLHILGYNIAPGNGPLENRLARLREHRKDRN
                     AAICSRLRELGVDVTLEEVQEEAKGEVVARPHIARVLIRKGYVQDMAGAFRVYLADGA
                     AAYIPRVRLSPAECIELIRQSGGVPVLAHPGQMGLAEEPLKAVLRELKEMGLWGLECL
                     SSHHRYEEIYQYLKLADSMGLFATAGTDFHGWNRPGVQLGIPVGEDFLPWARLQVAL"
     gene            3089..4126
                     /gene="ltaE"
                     /locus_tag="PLY83_03335"
     CDS             3089..4126
                     /gene="ltaE"
                     /locus_tag="PLY83_03335"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HOV29105.1"
                     /translation="MKTIDLRSDTVTRPTPGMRKAMAEAVVGDDVYGEDPTVRKLEET
                     AAEMTGHEAGLFVTSGTQGNLLALLCHCRRGEAVILGNQSHILNYEGGGMAALGGILP
                     LSTEDAGGLPETSSVLRWVRPANVHFAPAKLLCLENTHNREGGNASAPLAFQKLVTAA
                     KDHGLAVHLDGARIFNAAIAWDVDVKTYTGLVDSVQFCLSKGLGAPVGSLLCGRKSFI
                     EEARHWRKVVGGGMRQSGVLAAAGLVALKENLSRLSEDHENAAILAERLEESEHLLVE
                     TCPRRTNMVYFQICKEGLTAEELARRCRLKGVLFNPVSAKRVRLVTHLDVSRSDVREA
                     ARIILEEAANR"
     gene            4123..5487
                     /locus_tag="PLY83_03340"
     CDS             4123..5487
                     /locus_tag="PLY83_03340"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOV29106.1"
                     /translation="MREVRGNRTIEDMCAWLIEKALKGGASDADVFYTEGQSRHISLR
                     EGVPEEDVLGFGQSVTLRSVLPDRRQGLASVNHFKREDLLELVDWSLSNAALSEPDPY
                     LEIGEGAWASDGEDLGIEDPELEKLTHENRIRACVEMTEAARSADRRVVSVRSATWSD
                     ARGKIYLANSRGFGAWQSGTTVSCGVAVVMQENDSFEMGAFGEDSRCWRKLDPGAVAR
                     EAVRKTGMILGGKPIETARMTVFLDPESCADFIDAIGDLFLGPNVLRNKSLLRGKLGT
                     AVASPSFSLVDDGRLFGGMASGPFDGEGVQTRRTPLITGGVLENYLYDLRSARESGVA
                     STGNAVRGSGSAPDADCSNLFPVPGKKDPRTLFLDAGRGLLVTEIMGLHTINPVSGDF
                     SLGIKGVLMEGGEPVRPVAEVTVAGNLADWLLQIGELGNDLRFFGSTGGCTMVINDVA
                     VAGS"
     gene            5474..7081
                     /locus_tag="PLY83_03345"
     CDS             5474..7081
                     /locus_tag="PLY83_03345"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOV29107.1"
                     /translation="MPVRRFFLCLLLLCVASAPAFSAETGWTLWKNNGIAGVVKIRDE
                     GSTVKVGLKNMASLLGCVASVKGDRLIVTCGNRKLEFVLGAAAARIDGGQILPMADAV
                     TAEEGEWWGENRGALRLYEALLSGGNGSVRLRFAGSAALPEKEAATVASVPPAAAESA
                     ELGKSLYGPLPRIDKIRWSRTAEKIRAVLDLSSPLEPGVKEAPGEVEITLAAAAARSI
                     SGVPSPYPGEVSVAFTQYGDKTIVRFQHRAASIKTFSLHKPERFVLDFFSTGTVAPGV
                     EPAASVEPAPLNPAEPALPPAAAPVPAGRAPGGKPFLVVVDAGHGGHDPGAVAGGINE
                     KDINLRAALRLAALLREAGIEARLTRKDDTYLRLNERTDLANRWNADLFVSLHCNALP
                     RGRHARGVEIYLMALPTDKDAMRLALYENRELGKNNTIEESDAKTNLLLRILGEMEQN
                     AKIGQSTRLAESLFNTGRRTGLQMSRVAQAPFFVLRGAAMPAVLVEMGYLTEPEDRRL
                     LNDPAFLDRLLRALVSGMSEYLNTLSR"
     gene            7098..7733
                     /locus_tag="PLY83_03350"
     CDS             7098..7733
                     /locus_tag="PLY83_03350"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOV29108.1"
                     /translation="MKDETDSRLRERTVRRQPAETKAPWILRLVSWSAVMLLLFTLGY
                     FGTGLVLKWVDSKGGPQETTVVSGKEPVLGETPKAPQNAYRVYSLKGNRLSESRVETA
                     GGLMESDMREVLQSLFSLLQTEGVLDPLSAVLHVFRAGDLLYLDVNDACVRSIASLPP
                     EKANLVMTGVVRTIIENFRPVTRVRFLVNGRESTETKPVNLAVAWQLARKP"
     gene            7758..8516
                     /gene="rph"
                     /locus_tag="PLY83_03355"
     CDS             7758..8516
                     /gene="rph"
                     /locus_tag="PLY83_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOV29109.1"
                     /translation="MDHTDFRHDGRKADQLRPVSIRRQVNLYAEGSSLIEWGNTKVLC
                     TASVEEKVPPFLKGGGRGWVTAEYAMLPRATLTRTPRDSVRGIGGRSHEIQRLIGRSM
                     RSVIDLAALGERTIWVDCDVLQADGGTRTAAVTGAFVAVFDALRGLKEREVIGQLPVR
                     GFVAAASAGRVEGTVLLDLDYFEDSRAEVDFNVVMTHKGEFVEVQGTGESGPFDRQSL
                     LEMLALAEMGVRRLIELQKDCLDLTEEERDAFSF"
     gene            8500..9132
                     /gene="rdgB"
                     /locus_tag="PLY83_03360"
     CDS             8500..9132
                     /gene="rdgB"
                     /locus_tag="PLY83_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOV29110.1"
                     /translation="MPFLSEVVLASGNAHKHRELSALFAPIGFSLVFGPERLALEVEE
                     KGKTYAENALLKATAWSQALGMPAVADDSGLEVQDLGWAPGLFSARVASSDPERIAWL
                     LDRLRGKECRRARFVACIALVWPEETQTRVFLSEGICWGRIATEAAGSEGFGYDPVFI
                     PDRFHATFAELGEELKSRISHRAIACRALRDMLEQPDMVKSLSVRSGETD"
     gene            9145..9378
                     /gene="secG"
                     /locus_tag="PLY83_03365"
     CDS             9145..9378
                     /gene="secG"
                     /locus_tag="PLY83_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOV29111.1"
                     /translation="MKIVFGLIHIVLCVALIGVIMLQHRKSGGFQGIFGGGTQADMAG
                     GQWQRLSAMTKVTVGLMGLFMAMSLVLVLLSYR"
     gene            9398..10471
                     /locus_tag="PLY83_03370"
     CDS             9398..10471
                     /locus_tag="PLY83_03370"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOV29112.1"
                     /translation="MAGSRQLESMKDVHDLTVSRPRLFSATHEEILQGETTDVYFVKT
                     LDLLRACGYADRLVVAEVFARGSGIFAGLPEVLELFREKPGLVVESLAEGDPFSTKET
                     LLRIRGPYSSFGMYETVLLGMLASASGWATAARECVEAAGGKPVLSFGARHVHPAVAP
                     VMERAAVVAGGCQGASCILGAKLAGVEPRGTIPHAAILIVGDTVKVAEVYDRFLPEGE
                     PRIILVDTFKDEAEESLRVAASLKGSLSGIRLDTPGERGGVTAELVREIRWRLDQAGY
                     EKVQIIVSGGLTPERIRLLSNAGADAFGVGSYIAHATPRDMTMDIKMVEGTPIAKRGR
                     LPGLIENPGLKQVLNTASVQKKS"
     gene            10630..11061
                     /gene="rplM"
                     /locus_tag="PLY83_03375"
     CDS             10630..11061
                     /gene="rplM"
                     /locus_tag="PLY83_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOV29113.1"
                     /translation="MIGKRSFLARKETVQHKWYVVDAADKPIGRVAVKVARILSGKNK
                     PIYTPHVDTGDFVIVINADKVVLTGKKSERERIYWHTGYPGGIKSVKMGEMLAKKPER
                     LFERVVKGMLPKTRLDYIRKLKVYAGPNHPHAAQMPEKLED"
     gene            11103..11489
                     /gene="rpsI"
                     /locus_tag="PLY83_03380"
     CDS             11103..11489
                     /gene="rpsI"
                     /locus_tag="PLY83_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOV29114.1"
                     /translation="MAYNWGTGRRKEAVARVRIRPGEGKFLINNREVSDYFPRSTWQL
                     IVYKPLKVGGVEGKVDVFVRAHGGGLTGQSGATSLGIARALLKLNPELRPALKRAGLL
                     TRDPRMVERQKFGQKGARARNQYSKR"
     gene            11612..12241
                     /locus_tag="PLY83_03385"
     CDS             11612..12241
                     /locus_tag="PLY83_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOV29115.1"
                     /translation="MPKVEEIESKELTRRKILDVARRIFALHGFEGASVDAIVKASGL
                     SKGALYWHFPGKLELFREIMGEEARLILRHFEIPLEMKGEFDPVQFLIEKGGTLIDEF
                     SDNQERRLLWVDLTVVAQRGDPQSKKLAGEMIDRVLDAVLPELDRTFRGSSVGGTFLS
                     PGERLTCLTHAFGGLVMNLGLRLKAEEAKRYWEAMVRMLLEEGGRNEKP"
     gene            12228..13586
                     /locus_tag="PLY83_03390"
     CDS             12228..13586
                     /locus_tag="PLY83_03390"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOV29116.1"
                     /translation="MKSRKWILLLIFAIICALVLIAGHLAWGAEPAGTLTLDEALRIA
                     REANPLILAAQARVDQARGRLIQARAEQFPQVNATLGYQKLDERPETVAFGPGGAPIG
                     IVPLGYENTWQAALNLSQVLYSGGSLSARTEAERLTVSARESERDRTVQAVENGVRNA
                     FYGLQRSQSRLEVAKEALSLAKEHLKQVEAFYRAGVVAQNEVLRVQVAVSTSTLNLIR
                     AESAVKVAWKQLERVVGKSLEGQVAVPSGDADVETFPGFEDPLSRALNQRPELKGLAA
                     AGKAAQMTVRAARGQLFPQVGFSASAAVADEDFFPSEKDDWSVSVFARLRLFDSGKVH
                     GQVVEARAAAEELLHRLEDLKRQVALEVAAARVNLEGALQRVKVASDAVAQAEEDYRM
                     ALKRYQAQVGTNIDVLDARLALIDAKNSRIDAVAEARTAYGDLLFAMGEGSTEGRKEA
                     SR"
     gene            13583..14746
                     /locus_tag="PLY83_03395"
     CDS             13583..14746
                     /locus_tag="PLY83_03395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOV29117.1"
                     /translation="MKLHIPETFKARRIGIVAGVALIFLLLILFRGYQQHQLNSKPVE
                     VEKIPVELATPEVRVFKEKVSFVANLEPEEQAAVVAKLAGKTVLRVLVDIGTPVKAGQ
                     VLAILDDSLLRPQLAQAEATVEKARAALSQASTQLATARKDFERYERLYNEKVISGQQ
                     YDHAKGQADVAEAGERLARKQLAEASAGLNQLSVLMGYHVLRAPVTGVIAARFIDPGD
                     TSSAEKPAFLINRQEFLKVRGSVPEAAFAKMKVGQEGSVTLDALGGKSFRAKVVRLSP
                     EIDPVTRTGQAELVLKSQEGTKPGMYARVELEVGEHQGLSVPREAVKTLPGTGERRVF
                     LERDGKAESRAVRIGAEEGDRVEVIEGLASGEAVIVTQSARIGEGTPVEVAKP"
     gene            14743..17847
                     /locus_tag="PLY83_03400"
     CDS             14743..17847
                     /locus_tag="PLY83_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOV29118.1"
                     /translation="MRLPEFSVKKPIFTSMIYAAIVLLGVVTLASMKVDLLPKIDPPV
                     ITVLTTWPGASAIEIEQRVSKKLEDQLGMIEGVSDLYSKSVDNLSVVSAKFKWGTDLD
                     VKVGDVRDAVNFAKRRMPSDIEEPILFRMSSGTVPVLEIALTAGASYPGLYHYADSVL
                     VEDLKRVPGVGQVLVYGGLRREIRVAMDLAKLEAYGLAPQAIAAALERENLNAPAGPL
                     KEGRIAFNVRVPGRFRDIAEIRGLVVGAWQGKPVHLGDVARVEDTFKELEMNGWVNDK
                     PSVILIVLKNSDANTMEVTNGVKKRLEELKAELPSDIETTILMDTSEYIGNSIRDLSH
                     SLYWGIVLVFLVTWAFLKRFTASIVVCAAIPFSLLITFTVMKLLGYTLNIMTLMALSM
                     AVGMVVDNAIVSTDQIIYHMEKGERRGIASILGASEVGGALLGSTTTTVVVLMPLIFV
                     QGLVGVFFTSLCVVMIVAVAISLFVSISFIPMAGSKAFSTETENLRIHGYTEKFLRKL
                     ESGYRDLLSWGLDRPAEIFGIAALFMALTIAGFKFIGTELTPEADTSEISITFRLPEG
                     TRLETTDALVREVVEYARKNVPEKKYVFGWDGQTEEGYGIATGSDEGSNVGTVGLKLV
                     DKAERDRSAFEVAEQIRKWLEKKPGIQKMTVLVTSPIQAMFLGAKPVNIEVYGDDLKS
                     TVQVAEEIKNRLARIPGAVDVTLSQKPDRPEIQVLIDREKAALLGVSTASIASTLRMY
                     FYGVETGESFWQGEDDYPIRIRLDSRQRDTMGVLDRLVVPSSTGKAVRLSTVARIVQD
                     SGPSEVNRKNRQRYVVVESNVQGRSLGQVTEAARKEIASMNLPPGTKIAFGGQVQEQG
                     DAFRQLGLLVLLGIILVYMVIAAQYEALLDPFIILFSVPFALTGVVMAFLLTGLYLSM
                     QAFLGVIMLVGIVVNNAIVLLDYVGLLRARGMRLREALLEAGERRLRPILMTMLAAFL
                     GMLPMAISRGQGAEMWRPLAVSVMGGLALSTLITLILVPTVYLVMETKLRRKPRFAEA
                     KGGE"
     gene            17849..18142
                     /locus_tag="PLY83_03405"
     CDS             17849..18142
                     /locus_tag="PLY83_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29119.1"
                     /translation="MKLIWILCNESIAEEVRAVLDATPVKGYTVWQGVLGTSSGEAHW
                     GDAVWPGKNWAFMAVDEDERSSRLIGLLGELKREDHVRRAGLKVFVQEVQEAL"
     gene            18205..18280
                     /locus_tag="PLY83_03410"
     tRNA            18205..18280
                     /locus_tag="PLY83_03410"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18238..18240,aa:Thr,seq:tgt)
     gene            18305..18390
                     /locus_tag="PLY83_03415"
     tRNA            18305..18390
                     /locus_tag="PLY83_03415"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18339..18341,aa:Tyr,seq:gta)
     gene            18409..18484
                     /locus_tag="PLY83_03420"
     tRNA            18409..18484
                     /locus_tag="PLY83_03420"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18442..18444,aa:Thr,seq:ggt)
     gene            complement(18463..>18667)
                     /locus_tag="PLY83_03425"
     CDS             complement(18463..>18667)
                     /locus_tag="PLY83_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29120.1"
                     /translation="IVPMFRCGFVLSKCSFAILVTLPAEVIMIQRYCPVGPDFIIRPF
                     MLKETRNVSPGSPISTPGADFRT"
BASE COUNT         3826 a         5105 c         6123 g         3613 t
ORIGIN      
        1 actgcggttt gaaccgtggg agagtagatc gccgccggaa acctttttca aaaaaccgaa
       61 ggaggggagt cccatctggg actcccctcc ttcgcatctt caatccctcc gccttcagtc
      121 ttcaaccatg caatggcaga gatcctcgtt ccgcccgcta ccgggagagc agcgaaggtg
      181 cgtgattgaa gtccgacccc ctcggtgaag cgcaagaagg attgaaagaa gggaagaggg
      241 ggtgatcccg atggaccggg acagttcctc cacggcgatt tcttctccgg tgctgacccg
      301 gttcaggatt tccttcccga tcaattcgat ccactgacgg gtcagttcct gcatttccgg
      361 ggtggcgctg gaagcgatct gggcggttct cgtgatcgaa tcgatgagcc gggcagctac
      421 tctggcgcaa agatccgcaa tctcctcggg atgagcggtt ctttcatttt ccataccggt
      481 gaaaccccct tgaaaaagat gagtggccct aagtaaaggc gtggggccgc tttttcatgc
      541 tatcatccac ggtagcatca ttccgtcaac gattcaagaa aacggaggat aaggggttca
      601 tggctatccg ggtgctcctt ttagggtttg gaaatgtcgg caggaatttc tgcaggctgc
      661 tcgaaaaaca ggaccccctg ctcggaaatg aggagcaggt tcttttggcg gggctcgcca
      721 ctcgcggcag gggtacactc tggtctggcg acgggatccc ggtttccagg ctgctggagg
      781 tcgaaaggca aactggccgg gtcgacgctc tttcccttcc aggctgcaaa aagggactgt
      841 ccgcgatgga cctggtccgc gagggggaat tcgacattct ggcagagtgc acatcaggaa
      901 acctgaaagc catggaatcc gaaccggggt tttcccacat ccgtgccgcg cttgaaagga
      961 agaaaccggt tgcgaccacg aacaaggccg tggcggccct ccggttcgaa gaggtcgagg
     1021 cgctctcaag acaagcgggg gtcccctggc acttcgacgg aacggtcgtt gtcggcgccc
     1081 cttttctcga ttatgcacgc cgttccatcg tggatgcccg gatcctgggg tttgagggga
     1141 cactgagcgc gacctgcaat tatgtccttg actgcctcat gggaggggcc tccctggacc
     1201 aggccctcat ggacgcgcag cgttccggaa tggccgaatc cgatcccgca cttgacatcg
     1261 atggctggga ctcggcggcc aagctgatga tcatcgcgaa ggcgctcatg ggaaccccct
     1321 gccctgctct cggggagttc cccgtaaagg gaatccgggg actgacccgg gcggtgcttg
     1381 ccgaagcccg caggtcgggg ggaaaaatcc gcctcctggc ccggatcacg agatccgcgg
     1441 gaggagttga cctttctgtc ggtcctgaag tcctgcctcc ctcgcatcct ctttcccttc
     1501 tcttcggccc gacaaacggg gccgtgatcc tgaccgaaaa cctgggcgag atttgcctgt
     1561 cgggaacggg cagttccccg gcacaggctg cctttgcatt gtggcaggat atccggagaa
     1621 tcgcccgaag ggagaaacgc tgatggccgg ccgtctgggg atcatcgcgg acactcacgg
     1681 ggatctgctg ggctgggaat cggccgtccg cgcctggggg ccgatcgatc tgattctgca
     1741 tgcgggggat gtcctgaagc cctggaagag ccgcccgctg tcccatgtcg gcgggaaaag
     1801 ggccctggcc gaggcgatca acgcctgcga cgtgcccgtc ctgattgcca gggggaattg
     1861 cgatagcgaa gaagatgcgc aaatggtgaa atggcccatg gttgagccct ttttgtttct
     1921 ctggtggaac ggtcgcctct tggttgcggg acacgggacc gatttttcgg agatcaggga
     1981 gcgggggatt gccagcgagg ccgatgtcgt catcacgggg cacactcacg tcgcttccct
     2041 ggtgtgcgag agaaaaacat ggttcctgaa tcccggctcc gcaagcctcc ccatggggcg
     2101 tgaccctgcc agcgtggctc tcctggatgg agaggggatc cgcatcctga ctctcggaaa
     2161 tgagctcctt catttcgaac cctggtgaac cgagggtata atccttccat gatcctgatc
     2221 gacctgcata cccatagcac ctgctccgac ggcacgctga gccccgaagc cctggtccgg
     2281 gcggcaaagg ccaggaaagt ttccgtgcta agcctgaccg accatgatac gacggaaggg
     2341 gtcgccaagt tcctttcggc atgcgcccat gccggtatcc ggggcctttc cgggatcgag
     2401 ctttccgcgg aggccccgta cacgctgcat atcctgggct acaacatcgc tcccggcaac
     2461 gggccgctgg agaaccgcct tgccaggctc cgggagcaca gaaaggaccg gaatgcggcc
     2521 atctgttcgc gactgcgcga gctgggggtc gacgtcaccc tggaggaagt ccaggaggag
     2581 gccaagggtg aggtggttgc gcgcccccat atcgccaggg tcctgatccg caagggatac
     2641 gtccaggata tggcgggagc tttccgcgtc tatctcgccg acggcgcggc cgcctacatt
     2701 ccgagggttc gcctgtcgcc tgccgagtgc atcgaactga ttcggcagag cgggggggtc
     2761 cccgtgctgg cgcatccggg gcagatgggc ctggcggaag aacccttgaa agcggttcta
     2821 cgggaattga aggaaatggg cctttgggga ctggagtgcc tttctagcca tcatcggtac
     2881 gaagagatct accaatattt aaagcttgcg gattcgatgg gacttttcgc aacggccgga
     2941 accgattttc acggctggaa ccggcctggc gtgcagctcg ggatccccgt cggggaagat
     3001 tttctccctt gggcccggtt gcaggttgcc ttgtaaaaaa acaggatcga acaacttctg
     3061 gctagcaagt ctacgaggag gtgcaacgat gaaaaccatc gatcttcgaa gcgatacggt
     3121 gacccgcccg acccccggga tgaggaaggc catggctgaa gccgtcgtcg gtgatgacgt
     3181 gtacggggaa gaccccacgg tccgaaaact ggaagaaact gcagcagaga tgaccggtca
     3241 cgaggcgggc cttttcgtca cttctggaac tcagggaaac cttctcgccc tcctatgcca
     3301 ttgtagaagg ggggaggcgg tgatcctggg aaatcaatcg cacatcttaa attacgaagg
     3361 cggcggaatg gctgctctag gagggatcct tcccctgtct acagaggatg cgggcgggtt
     3421 gcctgaaacg agctctgttc tccggtgggt ccgcccggct aacgttcatt tcgcccctgc
     3481 gaaacttctt tgccttgaga acacccataa ccgcgaagga ggaaacgcct cagctccact
     3541 ggcgtttcaa aagctcgtga ccgcagcgaa ggatcatggc ttggccgttc acctggatgg
     3601 cgcccggatt tttaatgcgg ctattgcctg ggatgtcgat gtgaaaacct acacggggct
     3661 ggttgactcc gtgcaatttt gtctttcgaa gggactcggg gctcccgtgg ggtcgctgct
     3721 ctgcggcagg aagtccttca tcgaagaagc ccggcattgg cgaaaggttg tcgggggcgg
     3781 catgagacag tcgggggtgc ttgccgctgc cggcctggtg gccctgaagg agaatctctc
     3841 ccgattgtcg gaagatcatg agaacgctgc catcctggca gagcggctgg aagaaagcga
     3901 gcacctgctc gtggagacct gtccacgccg gacaaacatg gtttacttcc agatctgcaa
     3961 ggagggactg acggccgagg agctcgccag gcgttgccga ttgaaggggg tccttttcaa
     4021 tcccgtcagt gcaaagaggg ttcgcttggt cacgcacctg gacgtgtcga gaagcgatgt
     4081 tcgggaagcc gcaaggatca tcctggagga ggcagccaac cggtgagaga agtaagggga
     4141 aacaggacaa tcgaagacat gtgcgcctgg ctcatcgaaa aagccctgaa gggaggggcc
     4201 tccgatgcgg atgtgttcta cacggaggga caaagccggc acatcagcct gcgggaaggg
     4261 gtcccggaag aagatgtcct agggttcggg cagtcggtga cgttgcgctc cgtcctgccg
     4321 gacaggcgcc aggggttggc gtccgtgaac catttcaagc gggaggatct tctggaactg
     4381 gttgattgga gcctttccaa tgcggccctg tcggaaccgg acccctatct ggagattggg
     4441 gagggggcat gggcatccga tggcgaggac ctggggatcg aggaccctga actggagaag
     4501 ctcacccacg aaaatcgaat ccgggcctgc gtcgaaatga cggaagccgc ccgatctgcc
     4561 gatcgaagag tggtgtccgt gcgaagcgcc acgtggagcg atgcccgcgg gaagatctac
     4621 ctggcgaaca gccggggatt tggtgcctgg cagagcggga cgacggtttc ttgcggggtt
     4681 gcggtcgtca tgcaggaaaa cgattcgttc gagatgggag ctttcgggga agattcccga
     4741 tgctggagga agctcgaccc tggagcggtt gcgcgggaag cggttcgcaa gacaggcatg
     4801 atcctgggcg gcaaaccgat cgaaaccgca aggatgacgg tctttctcga cccggaaagc
     4861 tgcgcggatt tcatcgatgc aatcggagac ctgttcctcg ggccgaacgt cctgagaaac
     4921 aaatcccttc tccggggaaa actgggaacc gcggtggctt cgccttcctt ttcgctggtc
     4981 gacgatggcc ggctttttgg gggcatggcc tcgggcccgt ttgacggaga aggggttcaa
     5041 acccgaagga cgccgctgat caccgggggc gtcctcgaga attacctcta cgacctgcgt
     5101 tcggcccggg aatcgggagt cgcctcgacg ggcaacgcgg tcaggggaag cggaagcgct
     5161 ccggacgcgg actgctccaa cctcttcccg gtccccggga agaaagatcc caggaccctt
     5221 ttcctggatg cggggcgggg attactggtc accgagatca tgggtttgca tacaatcaat
     5281 cccgtgagcg gggatttctc ccttgggatc aaaggggttc tcatggaagg aggagaaccg
     5341 gtccggcccg ttgcggaagt gacggtcgcc ggaaacctgg ccgactggct tcttcaaatc
     5401 ggggaactgg ggaacgacct ccgctttttc gggagcacgg gcggctgcac gatggtgatc
     5461 aacgatgtcg cggttgccgg ttcgtaggtt tttcctctgt ctccttttgc tttgcgtagc
     5521 ctcggcaccg gctttttctg ccgaaacggg ctggaccctg tggaagaaca acgggatcgc
     5581 cggtgtggtg aagatccggg acgaaggatc gacggtcaaa gtcggtctca agaacatggc
     5641 ctcccttctc gggtgcgttg cgtcggtcaa gggcgaccgc ctgatcgtga cctgcggaaa
     5701 ccgcaagttg gaattcgttc tcggggccgc cgccgcaagg atcgacggag gccagattct
     5761 tccgatggcc gacgccgtga ccgcagaaga gggggaatgg tggggggaaa acaggggagc
     5821 cctgcggctt tacgaggcgc ttctttccgg tgggaatgga tccgttcggc ttcgtttcgc
     5881 cgggtccgca gcgcttccgg agaaggaggc tgcaacggtt gcgtcggtac ccccggcggc
     5941 tgcggaatcc gccgagctgg ggaagtccct ttatgggccc ctgcccagga tcgataaaat
     6001 ccgctggagc aggacggcag aaaagatccg ggcggtcctc gatctttctt cgccgcttga
     6061 gcccggtgtg aaggaggctc caggagaggt cgaaatcaca cttgcggccg ctgcggcccg
     6121 ctcgatcagc ggggtgcctt ccccctaccc gggggaagtc tcggtggcct tcacccagta
     6181 tggggataaa acgatcgtcc gtttccagca tcgcgcggca tctataaaga ctttttccct
     6241 ccacaagccc gagcgcttcg tgctggattt cttttctaca gggactgtcg ccccaggggt
     6301 tgaacccgcc gcatcggtcg aaccggctcc cctgaacccg gccgaaccgg cgctgcctcc
     6361 tgcggctgcc cccgtcccgg ccggcagggc acctggcgga aaaccgttcc tcgtggttgt
     6421 ggatgcgggg catggggggc acgatcccgg cgccgtcgcg ggcggaatca acgaaaagga
     6481 catcaacctg agggcggcgc tccggcttgc ggccctgctc agggaggcgg gcatcgaggc
     6541 caggctcacc cggaaggatg acacctacct tcgcctgaac gaacggaccg atctagccaa
     6601 ccggtggaat gccgacctct ttgtaagcct tcactgcaat gccctcccta ggggcagaca
     6661 tgcccgcggc gtggagatct acctgatggc tctgccgacc gacaaggatg ccatgaggct
     6721 ggcgttgtat gaaaaccggg agcttggcaa gaacaatacg attgaagaat ccgacgccaa
     6781 gacgaacctg cttcttcgaa ttctggggga aatggagcaa aacgcaaaga tcgggcagag
     6841 tacccggctt gcggaaagcc ttttcaacac tggacgccgc acgggcctgc agatgtcgcg
     6901 tgtggcccag gccccgtttt tcgtccttcg aggggcggcg atgccggctg tgctcgttga
     6961 gatgggatac ctgaccgagc cggaagaccg gaggctgctc aacgatccgg ctttcctgga
     7021 ccgcctgttg agagccctgg tttcggggat gtccgaatac ctgaataccc tatcccgtta
     7081 aggagttgag gacaggaatg aaagacgaga cggattcccg cctgagggag cgaacggttc
     7141 gaaggcagcc ggcggagaca aaggctccat ggattttacg gcttgtcagc tggtcggccg
     7201 tgatgcttct tctttttacg ctgggctact tcggaaccgg actggtgctg aaatgggtgg
     7261 acagcaaggg ggggccgcag gaaaccacgg ttgtctccgg caaggagccc gttttggggg
     7321 aaacaccgaa ggcgccacaa aacgcgtacc gcgtttattc gctgaagggg aaccggttga
     7381 gcgaaagccg ggttgaaacc gccggaggat tgatggaatc agatatgcgg gaagttctcc
     7441 aaagcctttt ctcccttctt caaacagagg gggtcctgga tcccctgtcg gcggttcttc
     7501 atgtcttccg ggcgggggat ctcctatacc tcgacgtgaa tgacgcgtgc gtccgatcga
     7561 ttgcctccct gccgccggag aaggccaacc tggtcatgac cggagtcgtg aggacgatca
     7621 tcgaaaactt ccgaccggtc acacgggtgc gcttcctggt caacgggagg gaaagcaccg
     7681 agacaaagcc cgtcaacctc gcggttgcgt ggcagctggc ccgcaagccc tgagcggcgc
     7741 caaacaggag gatcgccttg gatcatacgg actttcggca cgatggacgc aaagcggatc
     7801 agttgcgccc ggtttcgatt cgccgccagg taaacctgta cgcggaaggt tcctccctga
     7861 tcgagtgggg aaacacaaaa gtcctttgca ctgcttccgt cgaagaaaaa gtcccgccgt
     7921 ttctgaaagg tggcggtcga ggctgggtga cggccgaata cgccatgctg ccgcgcgcga
     7981 ccctcacccg taccccgaga gattcggtcc ggggaattgg aggaagaagc catgaaatcc
     8041 agcgcctgat cggacgttca atgcggtctg tcatcgatct tgccgccctt ggagaaagga
     8101 cgatctgggt cgattgcgat gttctgcagg cggatggggg aacacggacg gctgccgtca
     8161 cgggggcctt cgtggccgtg ttcgacgcgt tgcggggatt gaaggagcgc gaggtcatcg
     8221 ggcagctccc ggtccgcggg ttcgttgccg cggcaagcgc cgggcgggtg gaagggacgg
     8281 tcctcctgga tctggactat tttgaagaca gccgggcgga agtggatttc aacgtggtca
     8341 tgacgcacaa gggggaattc gttgaggtcc agggaaccgg cgaaagcggg ccgttcgatc
     8401 ggcaaagcct cctggaaatg ctcgctttag ccgaaatggg ggttcgacgt ctcatcgaac
     8461 ttcaaaagga ttgcctggac ctgacggagg aagaacggga tgccttttct ttctgaggtt
     8521 gtgctggcca gcgggaatgc ccacaagcac agggaattga gcgccctttt cgccccgatc
     8581 gggttttccc tcgttttcgg gcctgagagg cttgccctcg aggtcgagga aaagggaaag
     8641 acctacgccg agaacgccct cctgaaggcg acggcctggt ctcaggcgct ggggatgccg
     8701 gctgtggcgg acgacagcgg gttggaggtc caggacctgg gctgggcccc cgggcttttt
     8761 tcagcgcggg tagcctcctc ggatcctgag cgaatcgcct ggctgctcga ccgcctgcgc
     8821 ggaaaagaat gccggagggc ccggttcgtg gcttgcatcg ccctggtctg gccggaagaa
     8881 acccaaacac gggttttcct ttcggagggg atctgctggg gccgaatcgc gacggaagcc
     8941 gcgggttccg aaggcttcgg ctatgacccg gttttcatcc ctgaccggtt ccacgcgacc
     9001 tttgccgaac tcggggaaga gctgaaatcc cgcatttccc accgggcgat cgcctgcagg
     9061 gcgctgcggg atatgcttga acagccggat atggtaaaat ctctttctgt ccgctctgga
     9121 gaaacggatt agggggtgct ttgcttgaag atcgtgttcg gtttgatcca cattgtcctt
     9181 tgtgttgccc ttataggggt cattatgctg cagcaccgga aaagcggggg attccaggga
     9241 attttcggcg gcggcaccca ggcagacatg gcaggcggcc agtggcagcg cctgagcgca
     9301 atgaccaagg tcacggttgg cctgatgggg ctgttcatgg ccatgtccct ggtcctggtc
     9361 cttctctcct acagataggc ggaaaggatt cctcccgatg gctggatccc gccagctcga
     9421 atcgatgaag gacgtccatg acctcacggt ttcccggccg cggctgttca gcgcgactca
     9481 cgaagagatc ctccaggggg aaaccacgga tgtctacttc gtaaagacgc ttgacctgct
     9541 tcgggcctgc ggatatgccg acaggcttgt tgttgccgag gtttttgccc gggggtcagg
     9601 gatcttcgcg gggttgccgg aagtgctgga gctctttcgg gaaaagcccg ggctcgtggt
     9661 ggaatccctt gcggaaggag accccttctc cacgaaggaa acgctcctga ggatccgcgg
     9721 accctattcc agcttcggga tgtatgaaac ggtcctcctg gggatgctgg caagcgcaag
     9781 cggttgggcc accgcagcca gagagtgcgt ggaagcggcg ggaggcaagc cggtgctgag
     9841 tttcggagcc cgtcatgtcc accctgccgt tgcgccggtc atggaaaggg cggcggttgt
     9901 tgcgggaggc tgccaggggg caagctgcat cctgggcgca aaactcgcag gcgtggagcc
     9961 gagggggacg atcccccacg cggcgatcct tatcgtcgga gatacggtga aggttgcgga
    10021 agtctacgat cggttcctgc ccgaaggcga gccgagaatt atcctagttg acaccttcaa
    10081 ggacgaagcc gaggagagcc tgagagttgc ggcaagcctg aaggggagcc tttcgggaat
    10141 tcgtctggac accccggggg aacgcggggg agtcacggcg gaactggtcc gggaaatccg
    10201 gtggcgtctc gaccaggcag gatacgaaaa ggtccagatc atcgtgtcgg gaggactgac
    10261 cccggagcgg atccgccttc tttcaaacgc cggggcggat gcgttcgggg tgggaagcta
    10321 catcgcgcat gcgaccccca gagacatgac catggacatc aagatggttg aaggaacacc
    10381 gatcgcaaaa cggggacgac tgccggggct cattgagaat cccggattga aacaggtgtt
    10441 gaacaccgcc tcggttcaga aaaagtcgta agacaaaaag ggaaaacaat caggttttcg
    10501 aagcgggcgc cctaccacac acccgtggga gagaaccttt cttgacagtg ccggatgcct
    10561 ccggtatgat tcattagctt tttcgaaagg gtattggtaa ctggagccca tgaaggagtg
    10621 agtggtgaaa tgataggcaa gcggagtttt cttgcgagaa aagagaccgt tcagcacaag
    10681 tggtatgtcg tggacgcggc cgataagccg attggacggg ttgccgtcaa ggtggccagg
    10741 atcctgtccg gaaagaacaa gccgatctat acgccacatg tcgataccgg cgattttgtc
    10801 atcgtcatca acgctgacaa agtggtcctg acgggcaaaa agtcggagcg ggaaaggatt
    10861 tactggcata ccggataccc cggggggatc aagtccgtca aaatgggaga aatgctcgcc
    10921 aaaaagccgg aaaggctttt tgagcgcgtc gtgaaaggca tgctcccgaa aacccggctg
    10981 gattacatcc gcaagctcaa ggtctatgca gggccgaatc atccgcatgc ggcccagatg
    11041 cccgagaaac tcgaagactg atccgtcgtt ttcgaaaaga ggagggagca caggtgcaat
    11101 ccatggcata caactggggc actggaaggc gaaaggaagc ggtcgcccgt gtccggatcc
    11161 gtcccggtga agggaaattt ctcatcaaca accgcgaagt cagtgactac ttcccgcgct
    11221 cgacctggca gctgatcgtc tacaaacctc tcaaggtcgg gggagtggaa ggcaaggtcg
    11281 atgtattcgt ccgggcccac ggaggaggtc tcaccggtca gtcaggcgct acctctctcg
    11341 ggattgcccg ggcccttctc aagctgaacc cggagctgag accggccttg aagcgcgcgg
    11401 gcctgctgac ccgggacccg aggatggtcg agcggcagaa gttcggccag aagggtgccc
    11461 gtgcacggaa ccagtactcc aagcgttaag agattcggtt atctcacgat gggacctcga
    11521 gcgaggtccc atttttttgc ggaccttctt gaccgaccgt tcggtctgta atatcctacg
    11581 ctccgaagcg aggaaacagg agggggagga cgtgccgaag gtcgaagaaa tagaatcgaa
    11641 ggaactgacg agacggaaaa tcctggatgt ggcgaggaga atcttcgcac ttcacggttt
    11701 cgagggggct agcgtcgacg ccatcgtcaa ggcttccggc ctcagcaagg gagccctgta
    11761 ctggcatttc ccggggaagc tggaattgtt cagggaaatc atgggagaag aggcaaggct
    11821 gatcctgagg catttcgaaa ttcccctgga aatgaagggg gagttcgacc cggtgcaatt
    11881 cttgatcgaa aaaggcggga cgctcatcga cgaattctcg gataaccagg aaaggcgcct
    11941 gctgtgggtc gacctgacgg tcgtcgcaca gagaggggat ccacaatcga aaaaacttgc
    12001 cggagaaatg atcgaccgcg tgcttgacgc tgtcctgcct gaattggacc ggaccttccg
    12061 ggggagttcc gtcggaggaa ctttcctaag tccgggggaa agattgactt gtctgacgca
    12121 cgccttcggg gggctcgtga tgaaccttgg ccttcggtta aaggccgaag aggcaaagcg
    12181 gtattgggaa gccatggtaa ggatgctgct ggaagagggg ggtaggaatg aaaagccgta
    12241 agtggattct tcttttgatt ttcgcaatca tttgtgccct ggttttgatc gcggggcatc
    12301 tggcctgggg ggcggaaccg gcgggaacgc tgacgctgga cgaggccctg aggattgccc
    12361 gggaggcgaa tcccctgatt ctcgccgctc aggcccgggt ggaccaggcg cgcggaagac
    12421 tgatccaggc cagggcggag cagttccccc aggtgaatgc cacactcgga taccagaaac
    12481 tagacgagcg accggaaacg gttgcttttg gtccgggcgg agccccgatt ggaattgttc
    12541 ctctagggta cgaaaacacc tggcaggctg ccctgaattt gagccaggtc ctctatagcg
    12601 gggggtccct ttcagcgcgc acggaagcgg aacggctgac ggtttcggcc cgggaaagcg
    12661 agagggatag gaccgtccag gccgtggaga acggggtccg aaatgccttt tatgggctcc
    12721 agcggagcca atccaggctc gaagtggcga aagaggcgct ttctcttgca aaagaacacc
    12781 tgaagcaggt cgaagccttc taccgtgccg gggtggtggc gcagaacgag gtcttgaggg
    12841 tccaggtcgc ggtatccacc tcgacgctga acctgattcg cgctgagagc gcggtgaagg
    12901 ttgcctggaa gcagctggaa agggtggttg ggaaatcgct cgaagggcag gttgcggtac
    12961 cctcgggaga tgcggacgtg gaaaccttcc cgggattcga ggatccgctt tcgagggcct
    13021 tgaaccagag gcccgagctg aaaggtcttg ctgcggcggg aaaagcggcg caaatgactg
    13081 tccgggccgc cagggggcaa ctttttccgc aggtcgggtt ttccgccagc gccgctgttg
    13141 cagatgaaga tttcttcccc tcggaaaagg acgattggag cgtttcggtt ttcgcccgcc
    13201 tgaggctctt cgactcgggg aaagttcacg ggcaggtggt ggaggcaagg gctgctgccg
    13261 aggaacttct gcaccggctg gaggatctga agcgccaggt cgcgctcgaa gtggccgcgg
    13321 cccgggtcaa cctggaaggc gccctccaga gggtgaaagt cgcctcggat gcagtagccc
    13381 aggcggagga agactaccga atggccctca agcgctacca ggcacaggtg ggcaccaata
    13441 tcgacgtcct ggatgcgcga ctggccctga tcgatgcgaa aaactcgagg atcgacgccg
    13501 tggcggaagc caggacggct tacggggatt tgcttttcgc catgggggaa ggcagtaccg
    13561 aaggacggaa ggaggcttcc cgatgaagct gcacataccg gaaacattca aagcccgccg
    13621 catcggcatc gttgccgggg tggcgctgat cttcctcctg ctgattcttt ttcgcgggta
    13681 ccagcaacat caactcaact cgaagcctgt cgaagttgaa aaaatcccgg ttgagctggc
    13741 gaccccggag gtccgggtgt tcaaggaaaa agtctctttc gtcgcgaacc tcgaaccaga
    13801 ggagcaggca gccgttgtcg caaagcttgc cgggaaaacg gttctccggg ttctcgtgga
    13861 catcgggact cccgtcaagg caggccaggt gctggcgatc cttgacgaca gtctcctgcg
    13921 cccgcagctt gcgcaggcgg aagccaccgt cgagaaggcc cgagcggctc tctctcaggc
    13981 ttcgacccag cttgcgacgg cccggaaaga ctttgagcga tacgagagac tctacaacga
    14041 aaaggtcatc agtgggcagc aatacgatca cgccaagggg caagccgatg tcgcggaagc
    14101 tggcgagagg ctggctcgaa aacagctggc ggaagcttcc gcgggtttga atcagctttc
    14161 cgttctaatg ggctaccacg tcctcagagc tccggttacg ggagtcattg ccgcccggtt
    14221 tatcgatccg ggggacacca gttctgcgga gaaaccggcg tttctgatca atcggcagga
    14281 gtttttgaag gttcggggct cggttcccga agctgcgttc gccaagatga aggtggggca
    14341 ggaaggttcc gtcacgcttg atgcgctggg aggaaagtcg ttccgggcga aggtcgttcg
    14401 gctttcaccg gagatcgacc cggtgacccg tacgggacag gccgaactgg tcctcaaatc
    14461 gcaggaagga acgaaaccgg gaatgtacgc ccgggttgaa ctggaagtcg gcgaacacca
    14521 gggactttcc gttccgcggg aggccgtcaa gaccctgccc gggacaggag agagacgggt
    14581 cttcctggaa agagacggca aggcggaaag ccgggcggta aggattggcg cggaagaagg
    14641 cgatcgggtt gaagtgatcg aagggctcgc ttcgggagaa gccgttatcg tgacccaatc
    14701 cgccaggatc ggcgaaggaa ctcccgtgga ggtggccaaa ccttgagatt gccggagttt
    14761 tccgtaaaga agccgatttt cacttccatg atctatgcgg ccatcgtcct gctcggtgtc
    14821 gtgacgctgg cttcgatgaa ggtggatctg cttccgaaaa tcgaccctcc cgttatcacg
    14881 gtcctgacga cctggcccgg ggcttcagca atcgagatcg agcagcgggt ttccaagaaa
    14941 ctggaagacc agctcgggat gatcgagggc gtgagcgatc tttactcgaa atcggtggac
    15001 aacctctcgg ttgtttcggc gaagttcaag tggggaacgg acctggacgt gaaggtcggg
    15061 gacgtgcggg acgccgtcaa cttcgccaag cgcagaatgc cttccgatat cgaggagccg
    15121 atacttttcc ggatgtcctc gggcaccgtt cccgtcctgg aaatcgccct gacggcggga
    15181 gcttcctacc ctgggctgta tcactatgcc gacagcgtcc tcgtggagga tctcaagcga
    15241 gttcccgggg tcggacaggt tctcgtctac ggggggctgc gcagggagat ccgggtggcg
    15301 atggacctgg cgaagcttga ggcgtacggg ttggcccccc aggcgatcgc tgccgcgctg
    15361 gaaagggaaa acctgaacgc ccctgccggg cctctgaaag aagggcggat tgcctttaat
    15421 gtgagggttc ccgggaggtt cagggacatc gcggagatcc ggggactggt cgtaggggcc
    15481 tggcagggca agccggttca tctcggcgat gtggcgagag ttgaagacac cttcaaggaa
    15541 ctcgagatga acggctgggt gaacgacaaa ccctccgtga tcctgatcgt cctcaagaac
    15601 agcgacgcca acacgatgga agtgacgaac ggggtcaaga agaggcttga ggagctgaag
    15661 gcggaacttc cctcggatat cgaaaccacg atcctgatgg acacctcgga gtacatcggc
    15721 aactcgatcc gggacttgtc gcactctctt tactggggca tcgttcttgt ctttctcgtg
    15781 acctgggctt tcctgaagcg gttcaccgct tccatcgtcg tctgcgccgc gatccccttc
    15841 tcccttttga tcacgtttac ggtgatgaag cttctcggct acaccctcaa catcatgacg
    15901 ctgatggccc tttcgatggc cgtgggaatg gtcgtggata atgccatcgt ctccaccgac
    15961 cagatcatct accacatgga aaagggggag cgccggggaa tcgcttccat ccttggggcg
    16021 agcgaagtcg gaggggccct gctgggctcc acgacgacga cggtggttgt gctgatgccc
    16081 ctgatcttcg tccagggcct tgttggggtt ttcttcacgt ccctctgcgt cgtcatgatc
    16141 gtggcggtgg cgatctccct gttcgtttcc atcagcttca tcccgatggc cggcagcaag
    16201 gcgttcagca cggaaacgga aaacctcagg atacacgggt acacggaaaa attcctccgg
    16261 aaactcgagt ccggctacag ggacctcctt tcatgggggc tggacaggcc cgctgagatt
    16321 ttcggcatcg ccgcgctctt catggctctg acgatcgccg gattcaagtt catcgggacg
    16381 gaactcaccc cggaggcaga tacgagcgag atctcgatca ccttccggct tcccgaggga
    16441 acgcgcctgg aaacgacgga tgcgctggtc cgggaagtcg tcgagtatgc gagaaagaac
    16501 gtccccgaga agaagtatgt cttcgggtgg gacgggcaga cggaagaggg ctacgggatc
    16561 gccacgggaa gcgacgaggg aagcaacgtc ggaacggtgg ggttgaagct ggttgacaag
    16621 gccgaacggg accgctcggc ttttgaagtg gccgaacaga tccggaaatg gcttgaaaag
    16681 aagccgggca tccagaagat gaccgttctc gtcacctcgc cgatccaggc gatgttcctg
    16741 ggcgccaagc cggtcaacat cgaagtgtac ggggacgacc tgaagtcgac ggtccaggtg
    16801 gcagaagaga tcaaaaaccg gcttgcgagg attccggggg cggtggacgt gaccctgagc
    16861 cagaaacccg accgccccga aatccaggtc ctgatcgacc gggaaaaggc tgccctgctg
    16921 ggggtcagca cggcctccat cgcttccacc ctccgcatgt acttttacgg ggtcgaaacc
    16981 ggggaaagct tctggcaggg cgaggacgac taccccatcc ggatccgcct ggacagtcgc
    17041 cagagggaca ccatgggcgt cctggaccgc ctggtcgtcc cctcttcgac gggcaaggcg
    17101 gtgaggctct ccaccgtggc ccggatcgtc caggatagcg gtccttccga ggtgaaccgg
    17161 aaaaaccgcc agcggtacgt ggtcgtcgaa agcaacgtcc aggggagatc cctggggcag
    17221 gtcacggagg cggcccgaaa ggagatcgct tcgatgaacc tgccgcccgg cacgaagatc
    17281 gctttcggcg gtcaggttca ggaacagggc gatgccttcc gccagctcgg tctcctcgtg
    17341 ctgctgggga tcatcctggt gtacatggtg atcgccgcac aatacgaggc actcctcgac
    17401 cccttcatta tcctgttcag cgtgcccttt gccctgaccg gcgtggtcat ggccttcctc
    17461 ctgacgggtc tttacctctc gatgcaggcc tttctcgggg tcatcatgct ggttgggatt
    17521 gtcgtgaaca acgccatcgt tcttcttgac tacgtgggac ttctgcgggc caggggcatg
    17581 cgtcttcgcg aggcccttct cgaagccgga gagcggcggc tacggccgat cctgatgacg
    17641 atgcttgcgg ccttcctggg gatgctcccc atggccatca gccgggggca gggggcggag
    17701 atgtggaggc cgctggcggt ttcggtgatg ggaggcctgg ccctttctac cctgatcacg
    17761 ctgatcctgg tcccgaccgt gtacctggtc atggaaacga agttgcggcg gaagccgcgc
    17821 tttgccgagg caaagggggg cgaatgaggt gaaattgatc tggatccttt gcaacgagag
    17881 catcgcggaa gaggttcgcg cggttcttga cgcaaccccc gtcaaaggct acacggtctg
    17941 gcagggggtc ctgggaacgt cctccgggga ggcccactgg ggggacgcgg tctggcccgg
    18001 gaaaaactgg gccttcatgg ccgtggatga agacgagcgc tccagccgcc tgatcggtct
    18061 tctcggggaa ctcaagcgag aagaccacgt ccggcgggcc ggactcaagg tgttcgtcca
    18121 ggaagtccag gaggctcttt gaaaaatgag aggtttttcc ggttgacaac ctcgttcctc
    18181 cagctataat agctagcgca cctcgccggc gtagctcagc gggtagagca gctgacttgt
    18241 aatcagcagg tcgtccgttc gatccggatc gccggctcca aaatgagagg ttgcagtgaa
    18301 gcgtggtagg gtggccgagt ggtcaaaggc agcagactgt aaatctgccg gctagcgcct
    18361 acgtaggttc aaatcctacc cctaccacca ctgacctata gagggtgggc cgacttagct
    18421 cagcaggtag agcacatcca tggtaaggat ggggtctccg gttcaagtcc ggaagtcggc
    18481 tccaggcgtt gaaatgggag atccggggct gacgttccgg gtctctttta gcatgaaggg
    18541 ccttatgata aaatcaggcc ctacgggaca ataacgttga atcatgatta cttctgcagg
    18601 gagggtaacc aggatggcga aggagcattt tgagaggacg aagccgcacc tgaacatcgg
    18661 cacgatc
//
LOCUS       DAOOVN010000021        17754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_2182_length_17754_cov_14.929375, whole genome shotgun
            sequence.
ACCESSION   DAOOVN010000021 DAOOVN010000000
VERSION     DAOOVN010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 17754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17754
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2182_length_17754_cov_14.929375"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..831
                     /locus_tag="PLY83_03430"
     CDS             124..831
                     /locus_tag="PLY83_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOV29121.1"
                     /translation="MDRILLVDDDKGLCELLGEFLESEGFAVEAVHNGEKAVNRALSE
                     RFSLVILDVMLPGLGGIEVLKAIRRQSAVPVLMLTARGEEVDRIVGLELGADDYLPKP
                     FNPRELVARIRAILRRGRMESAGEGAAGILRVADLVIDPAARHVSINGKTIELTTVEF
                     DLLEALAQSAGTAFSRENLVRQVLKRPFSPFDRSLDVHVSNLRRKIGAYPDGSERIKT
                     VRGVGYILAFPPEETRP"
     gene            828..2228
                     /locus_tag="PLY83_03435"
     CDS             828..2228
                     /locus_tag="PLY83_03435"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV29122.1"
                     /translation="MKGHALFLRIFLWFLGSMLLMAAVSVTLTVFMSRQGILLTGHQE
                     VLSDALDRHGPKLVETYEKKGWAEAFELTDKIRRETGLAIVLFDGNGVPIRLREGPPM
                     EFLNRLGREASRELAQGRTFLIRRETVSVYKRLTSSSGKTYLLVGLFRRPPSMARLIT
                     DNPKSVLVHLAGLLAAALVFCGLLAHHLSKPLVKLSRMACSVAEGQLDTPVDLSIRKR
                     RDEIGELARSLEAMKTTLTTLLEAQRRLIRDISHELRTPLARLGIALELARKKAGPAA
                     EESLDRIERESSILNEMIGQLLDLSRAEAEIGQDTPEPGDLLGLLEAVIDDANFEGRP
                     ANRGVILEAPETLRPIPMNPDLIRRAVENIVRNALRYTPEGTRVEVSVSEQEKDGRPG
                     VSIRIRDFGPGVPENELSRIFQPFYRTESARSRDTGGTGLGLAIAQRAALVHGGTIKA
                     ENLPEGGLAVEMWIPS"
     gene            2225..3352
                     /locus_tag="PLY83_03440"
     CDS             2225..3352
                     /locus_tag="PLY83_03440"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOV29123.1"
                     /translation="MKRRVPTGLLALLLLIGCTFPAAASARAVLVFGGDTSFGESYAK
                     SPEQKDLPEDVRYRTSLDALLPLLRDARQVLVNLETPLARPGETVVEGKAYVHWADPG
                     KTVRALKASGITAVSLANNHTMDLGTEGLEETLGVLDRAGLARTGAGLDLSEAERPLA
                     WEIPLGKRTARVAVFSGFEYRKNYDEKFEFYAAPAVPGVNPLDPERFKIAVHSLKSRI
                     PGVFVIAFPHWGKNYAWKTAEQVRLAHALVDAGADLVLGHGAHQVQEMEAYRGRWIVY
                     GLGNFLFHSPGRYGSRKAPPYSFVARLELAEKGETPQKTLRLYPIQCDNTVTGFRTRP
                     VNPREFTELRTLLIQRIDGKSASGISAKKDALGFHLEIALP"
     gene            3392..>4159
                     /locus_tag="PLY83_03445"
     CDS             3392..>4159
                     /locus_tag="PLY83_03445"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOV29124.1"
                     /translation="MRNSRLTLPVLAALGAVYFFWGTTYLGMKFAIETLPPFFMLSLR
                     FTAAGTLLLAWAASRGKLEATLGQLRGAALAGGVMLLGGTGLVAWAEQFVPSNIAAVV
                     IATMPLWMALVRWVALRGDRPGMASLAGLFLGFGGTLLLVRSTTAAGGTEEGHAAGLA
                     LLVLAAFLWASGSLLSRALEQPASPGFATSVQLFSGGICCLMAGLVTGEAGRIQIEAV
                     SFRSVAAIAYLAVFGSVVAFSCYTWLLKATDPALASSY"
     assembly_gap    4160..4259
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4260..4500
                     /locus_tag="PLY83_03450"
     CDS             <4260..4500
                     /locus_tag="PLY83_03450"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOV29125.1"
                     /translation="SCYTWLLKATDPALASSYAYVSPVVAVIAGWLLAGEKLNAGSLV
                     AAGVILASVALIIRGTAGPGKENLERKPAGPKGLP"
     regulatory      4551..4660
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4738..5544
                     /gene="thiD"
                     /locus_tag="PLY83_03455"
     CDS             4738..5544
                     /gene="thiD"
                     /locus_tag="PLY83_03455"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HOV29126.1"
                     /translation="MSIYRGVALTVAGSDSGGGAGIQADLKTFAALRVFGTSALTALT
                     AQNSLGVSAICGIPREMIRAQMEAVCGDFKPNAVKTGMLGSKEAILEVAGFLAGRCPF
                     LVVDPVMVATSGDSLLEPEAERTLGEILVPKADLVTPNVPEAEKLTGQPVRDLDGMIE
                     AAFCIARMGARGVLVKGGHMEGAVLRDVLLWEGRIRIFEEPRIPTQNTHGTGCTLSAA
                     IAAELAAGCGMEEAVLRGRSYMRSALAASFRGGRGRGCLGHAVSVPWIKP"
     gene            5541..6353
                     /gene="thiM"
                     /locus_tag="PLY83_03460"
     CDS             5541..6353
                     /gene="thiM"
                     /locus_tag="PLY83_03460"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: protein motif:HMM:NF006830.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HOV29127.1"
                     /translation="MSFPGKRLEDAWKALRKRRPLVYHLTNGVAMAEQAHVTLAAGAS
                     PVMSLCAEESESFARAADAVLINIGTPSAASGEIGERVALAAKEAGHPVVLDPVGFGA
                     TDLRNRLVRSLLKTRCITVLKGNASEICLLAGQAGSVRGVDRGLSPDPATAVLEVARR
                     ERLIAAATGKTDWVSDGERTWAVEGGSPMLESFSGSGCWLGSVVAACIGASGDPVAGT
                     LAALCAFGAAAEEAASLSRGPGSFRAHFLDSLHRLALGGTLPVAGRIREVSP"
     gene            6350..6997
                     /gene="thiE"
                     /locus_tag="PLY83_03465"
     CDS             6350..6997
                     /gene="thiE"
                     /locus_tag="PLY83_03465"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOV29128.1"
                     /translation="MSPLSCRLLLTVLVDPAGLPGRSVLEQAEGAIRGGATAIQLRSS
                     EASARELTALGKSLGDLCRKFDVLFIVNDRLDVALACGSNGVHLGQDDLPVDAAREIA
                     PRGFVIGVSAHDAKEAREAERLGADYLGVGAVFPTLTKKNARTIGIAGFRAVVESTTL
                     PCVGIGGITAGTALEVLRCGACGVAVHSAVAKAEDAEAAAREFRVFMEMGGPNRA"
     gene            6990..7733
                     /locus_tag="PLY83_03470"
     CDS             6990..7733
                     /locus_tag="PLY83_03470"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV29129.1"
                     /translation="MLEVLDFSLERGGAPILEGASLHLQRGGFGILVGRSGCGKTSFF
                     KALAGLLPRRAGQIRWRGKSVENLAGLAASMQQKDLLLPWATLEENVLLPALVSGRVG
                     PEERNRARELLKRFGLEGFEKFLPNQVSGGMRQRCALARTVLSGREILLLDEPLSALD
                     ALTRSHLQREIFRLQQEFGRTILMVTHDVEEALLLADRIFVFHGVHGGIEELLNLSGT
                     KPRETDAPAVVKNRKVLLEALERGHSHAA"
     gene            7723..8499
                     /locus_tag="PLY83_03475"
     CDS             7723..8499
                     /locus_tag="PLY83_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOV29130.1"
                     /translation="MQHRVTAFALSLLFLAAWETLCRFSEIPAFLLPAPSRIASTLAG
                     DFPLLLLHGVATLGEILLGVAGAVSAAIPLALAMFSSPCLERGLTPFLVASQAVPVFA
                     LAPILVFWLGYGIAGKVLVAGLVIFFPITVALLDGLKSCDPDLEDLFLLMGASFPARL
                     RLLYIPQALPWFFSGLRVGVTVSTIGAVIGEWVGAQKGLGYLMLQANARLRTDLVFSC
                     VALLSLIGLSLWFGLGWIETKLIPWRESLQFTPKARRSCN"
     gene            8496..9428
                     /locus_tag="PLY83_03480"
     CDS             8496..9428
                     /locus_tag="PLY83_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOV29131.1"
                     /translation="MKRILCSIVILLGLAAACPAAQEVKLMLDWVPNVDHLPIYVAKE
                     MGFFRKEGLEIRILAPSETTDALKLAAASKVDLAVGYAPQVIVSAAEGVPLQVVGRLM
                     EHPLSTLLFLEEKGIQSPSDLSGKRIGYTVPGVMDVLLDAFSKLNGVKGITPVNVGFS
                     IVQSLASGKVDAVMGGFRNYEAVEMIQQGMKPSAFPLEQWGIPDYDELVFVTSRNFAK
                     SNPQVIRSFRIALDKGIRASLANPGKALETYFRAVPEAPRNLEKEVLGKTLPFFARSQ
                     ELDPSRWKRFSAFALRWGLIGRAVEAERLLWKGR"
     gene            9434..9946
                     /gene="thiW"
                     /locus_tag="PLY83_03485"
     CDS             9434..9946
                     /gene="thiW"
                     /locus_tag="PLY83_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HOV29132.1"
                     /translation="MACTNLQLRKLVLAALFASVGVFLSGLSIPLGPTRCFPIQHAVN
                     VIAGVLLGPWWAAGAAFITSTLRLATGTGTFFAYPGSIPGALAVGLAASLLRRRRIYA
                     SLAEPLGTSIVGTGLSAAVIAPAVHSNATFAMLFAPFFASSVSGTVLGALVLVVLERS
                     RRAIEAAEEG"
     gene            complement(10012..10830)
                     /locus_tag="PLY83_03490"
     CDS             complement(10012..10830)
                     /locus_tag="PLY83_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29133.1"
                     /translation="MNRFWRNVLVLILAGTCVTAALPARADSWKEDRDDLYWTGRSVE
                     RLLSDDFLDGLRLTPRQREAIRDLRRDERFERRSERLADLEDLLGLIIVGEVLDSRRQ
                     SSLDRDLDFLLRTDDDFLYRFYQILEPDQRRLAWERMGRWYDRDGDRGWHFGRVRWEL
                     SRELERRLHLTPRQKREIDRILRDAAEEMRNRERRLWLLEREYFRNSWKPFSARSEAR
                     YRRDLLNARRDMLFLSYRVRERIRSVLDSRQRRIFDSWRGGIFEPYRKLYRGGK"
     gene            complement(10901..11878)
                     /locus_tag="PLY83_03495"
     CDS             complement(10901..11878)
                     /locus_tag="PLY83_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29134.1"
                     /translation="MKRWRGIGWMVALAAVFLGAALPAYAAAEFQDPVANMPSVREEI
                     DSLLTPELLNSLNLTRNQRDSLDELRRRDWDDRSDGRRDDRWENRRDERRYDSRYDRW
                     DGRDLGRLFGLFDLGRRGYGYDVRIERGDDDNFRTFLALLALGLYFSQSQDGYWDSDY
                     YRMMRLDDFLYLFMRILDADQRQTFILRFDRWYDDRYIYRRYDRRDDGYRWLPRDWDR
                     RLRLDNRQRRDMERVFRDLDDRYRDRERRYREMEKNYFRHSWDDRSGRDREEQRRRLF
                     EYRQQLRVPESEIRERLRPILNETQRRWLDSQERTQTPWMMRPPVKNDK"
     gene            complement(11996..13795)
                     /locus_tag="PLY83_03500"
     CDS             complement(11996..13795)
                     /locus_tag="PLY83_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HOV29135.1"
                     /translation="MAERMLLRVNLATGAISREPLNEEWTKAFLGGRGYATKLLYEEV
                     PAKTDPLSPENKLVLALGPLSGSNVPTGGRSMAVTKSPLTGLIACSNSGGQWGAEFAR
                     TGNEFLVVEGKAEKPVYLWIRDGETEIREAGSVWGKDTAFTTDRLLEEVGDEKAKVAC
                     IGPAGERLSRIACIINDRARAMGRSGVGAVMGSKNLKAVVVRGSAAWTAADPEGLKEA
                     LRVGMQKIKENGVTSQGLPTYGTAVLVNIINAAGSYPYRNFQSGTMAEADRQSGETLA
                     ATLLTGKRACYACPIACGRITKVGEKEGEGPEYESTWSLGSNCGVVELESITLANYLC
                     NELGLDTISAGGTVSAAMELFEKGYLPPEDLEGGPELKFGSGEALLYWLQKMGKAEGK
                     LGKLLAEGSARLCEAFGHPELSITVKKQELPAYDPRGIQGIGLTYATSNRGACHVRGY
                     MISAEILGIPQKLDPWTIDGKAEWTKIFNDLTAAIDSSGMCLFTSFALSAEDYAAFLK
                     SGTGRVGSVEEMMRCGERIWNLERLFNLREGLDPKKDDTLPKRLLEEPLPEGAAKGMV
                     SRVQEMVAEYYRVRGWDENGIPTEAKLKELGLK"
     gene            complement(13909..15018)
                     /locus_tag="PLY83_03505"
     CDS             complement(13909..15018)
                     /locus_tag="PLY83_03505"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOV29136.1"
                     /translation="MNVSAGETLLRFDDSTVSIPRERRLRKIRLELTSRCNLDCPMCF
                     LKGSGETRGDLEWKVLLDLFEQARRFPNLEWVHFGGIGEPLVHPRAIEAFQLAAEKGW
                     SLRLTTNGTLVDVDAARALVRAGLRIVDFSLEAEAVGLPGRDSDPGTVLEAMRTLEEA
                     RKAAGSPFPEIRAAIVLTASNLEKVLTLPRALREAGARGVSLSNLVPTSEGMRSLALY
                     PVSLEEEAVLQKKLFLAFQKEGLNVALPRFALSSERHCAFARSETLLVRWDGHVSPCI
                     RLSHGYDEWVLGRKKRVCAHSFGSVAEESLEAIWDSPAYTSFRWRLRRALYASCLDCN
                     LLPVCEYPKTTESDCWGDSPACSDCLWDRNLIRCP"
     gene            15361..16269
                     /locus_tag="PLY83_03510"
     CDS             15361..16269
                     /locus_tag="PLY83_03510"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOV29137.1"
                     /translation="MKGLEQNFKDILDNMLEAVYVTDRDRRIVYWNRRAEDLTGFMKE
                     EVIGRVCSENILGHVDRRGIPLCKGVCPLSETMADGKPRETMVFLHHKEGFRRPVRVR
                     TSPLIDSSGNITGGIEVFEDAMGYLALEEKIQLLESLSLLDPLTRIGNRRYLEEQLEE
                     KFKEFQISGLSLGLLFFDIDHFKRVNDTWGHVVGDRVLTVVASTLKHSLRIYDVLGRW
                     GGEEFLLLLPNLREGALEEVSDRLRQMVSASDIFLENSAEPLHVTVSGGATLARLGET
                     PQKALERADALLYESKAAGRNCITFG"
     gene            complement(16328..>17754)
                     /gene="ilvD"
                     /locus_tag="PLY83_03515"
     CDS             complement(16328..>17754)
                     /gene="ilvD"
                     /locus_tag="PLY83_03515"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOV29138.1"
                     /translation="PIGTPHMRYELVIRDVIASSIEIMANVQLLDGLVLLASCDSIIP
                     GVIIGGIRSGLPFIFLSGGPQLSARLEGRQVVMSELDQVVFGARYGKEDVSERMRFLE
                     DHVCPGPGACSLMGTANTMQILAEVLGLSLPGSATVPAVYAEKLRLGRRTGRRIVEMV
                     KEGLSVDKVLSRSNLLNAVKATLALAGSTNAVLHLVSIARELDIDLSLDDFDRLSREI
                     PVLSRVIPTGKKTVVDLHEAGGVPALLKELEKSLDLEAMTVTGKPLGEVLPRKGSLDP
                     TTLAGVGDPVFKSGGIAVLRGNLAPDGAICRTTTIPADRMRFRGPARVFSSDDAAHEA
                     VVRGDINRGDVVVIRMEGPRGAPGMREMMMTTDALVGLGLGSDVYVLTDGRFSGFTEG
                     CAIGHVAPEAFVGGPIAVIEEGDPIAIDIEGRTVNLDLPEPVILKRLTQWKQPEPKVK
                     RGILGLCAKTALQANLGGMLDDRC"
BASE COUNT         3126 a         5733 c         5388 g         3407 t
ORIGIN      
        1 cctgttgtct ttccgtaaag aatcgtaaag gctcctttac acgagtcaac ctttcttgac
       61 ggcaggtttc cggagaaaga aggatagaat ggtggaaaga accaggaaag ggggaaggac
      121 aagttggacc ggatcctgct cgtcgacgac gacaaaggtc tctgcgaact gctgggcgaa
      181 ttcctcgaaa gtgaggggtt cgcggtggag gccgttcaca acggggagaa ggccgtcaac
      241 cgggcgctat cggaacggtt ttcgctggtc atcctcgacg tcatgcttcc gggcctcggg
      301 ggaatcgagg tgctgaaggc catccgccgc cagtctgccg ttccggtcct catgctcacc
      361 gcccgcgggg aggaggtgga ccggattgtc ggactggaac tcggcgccga cgattacctg
      421 cccaagccct tcaacccccg ggagctcgtg gcccgcatcc gggccatcct cagaagggga
      481 aggatggagt cggccggtga gggcgcagcg gggatcctgc gggtcgccga cctcgtcatc
      541 gacccggccg cccgccacgt cagcatcaac gggaaaacga tcgagctaac gacggttgag
      601 ttcgatctcc tggaagccct ggcccaatcc gctggaacgg ccttctcgag ggaaaacctg
      661 gtccgccagg tgctgaaaag gccgttctcg ccgtttgacc ggagcctcga cgtccacgtg
      721 agcaacctgc gccggaaaat cggagcctac cccgacggct cggaacggat caagacggtc
      781 cggggtgtcg gctatatcct cgcctttccc ccagaggaga ctcggccttg aaaggacacg
      841 cccttttcct gaggattttc ctctggttcc tcggatccat gctgctcatg gccgccgtga
      901 gcgtaaccct caccgtcttc atgagccggc agggaatcct gctgaccggc caccaggagg
      961 tcctttccga tgccctggac cgccatggac ccaaactcgt ggaaacctac gagaagaagg
     1021 gatgggccga agcgtttgag ctgacggaca agatccgccg ggaaaccggg cttgccatcg
     1081 tcctgtttga cggaaacggc gttccgatcc gcctgcggga gggaccgccg atggaattcc
     1141 tgaatcggct cggccgggaa gcaagccgcg agctggccca gggccggacg tttctcatcc
     1201 ggagggaaac ggtttccgtc tacaagcgcc tgacgtcctc ctcggggaaa acgtaccttc
     1261 tcgtcggcct cttccgacgt cccccctcga tggcccggct gatcacggat aacccgaaat
     1321 ccgtcctggt ccaccttgca ggcctcctgg cggcggccct ggttttctgc ggactgctgg
     1381 cccaccacct ttcaaagccc ctggtcaaac tgagccgcat ggcctgctcg gtcgccgaag
     1441 ggcaactgga cacgcccgtc gacctttcga tccgaaagcg ccgggacgaa atcggggagc
     1501 tcgcccgaag cctggaggcc atgaaaacca ccctgaccac ccttctcgag gcccagcgaa
     1561 ggctgatccg ggacatctcc cacgagctgc ggacccccct ggcccgcctg gggatcgccc
     1621 ttgagctggc ccgcaagaag gctggacccg cggcggaaga gtccttggac cgaatcgagc
     1681 gggaatcttc gatcctgaac gagatgatcg ggcaactgct ggacctctcc cgggcagaag
     1741 ccgaaatcgg ccaggacacc cccgaacccg gcgacctgct cgggctgctt gaagccgtca
     1801 tcgacgatgc caacttcgaa ggccgtcccg ccaaccgggg ggtcatcctg gaggccccgg
     1861 aaaccctgcg gccgattccc atgaaccctg acctgatccg gagagccgtg gagaacatcg
     1921 tccgcaacgc cctccgctat acccccgagg gaacgcgggt cgaagtctcc gtgtcggagc
     1981 aggaaaagga cggccgcccg ggggtttcca tccgaatccg ggatttcggc ccgggcgttc
     2041 ccgaaaacga gctttcccga atcttccagc ccttctaccg aaccgagtcc gcccgatccc
     2101 gggataccgg cggaaccggc ctcggcctgg cgatcgccca gcgggcggct cttgtccacg
     2161 gcggaaccat aaaagccgaa aacctccccg agggcgggct ggcggtggaa atgtggatcc
     2221 cttcatgaaa cgcagggttc ccaccggcct tcttgcgctt cttttgctga tcggttgcac
     2281 gtttcccgct gccgcttcgg ctcgcgctgt cctcgtcttc gggggggaca cgtccttcgg
     2341 tgaaagctac gcgaaaagcc cggagcagaa agacctcccg gaagacgtcc gctaccggac
     2401 ctccctggac gctctcctcc cgctcctgcg ggatgcccgg caagtcctcg tgaacctcga
     2461 gaccccgctg gcacggccgg gggaaacggt cgtggaagga aaggcttacg tccactgggc
     2521 cgaccccgga aagacggtga gggcgctgaa ggcctccggg atcacggccg tcagcctcgc
     2581 caacaaccac acgatggacc tgggcacgga gggactggaa gaaaccctgg gagtcctcga
     2641 tcgggcaggc ctggccagga cgggggcggg cctggatctc tccgaagcgg aacggcccct
     2701 cgcctgggaa atcccgctgg ggaaaaggac ggcacgggtt gccgtgtttt ccggtttcga
     2761 ataccggaaa aactacgacg agaaattcga gttttatgcc gcacccgcgg ttcccggagt
     2821 caaccccctg gacccggaac ggttcaagat cgcggttcat tccctgaaat ccaggattcc
     2881 gggagtcttt gtgatcgcct tcccccactg gggcaaaaac tacgcctgga aaaccgccga
     2941 acaggtccgg ctggctcacg ccctggtcga cgccggcgcc gacctcgtcc tggggcacgg
     3001 ggctcatcag gtccaggaaa tggaagccta ccgtggaagg tggatcgtgt atgggctggg
     3061 caacttcctt ttccattccc cgggccggta cggttcgagg aaggccccgc cctatagctt
     3121 cgttgcccgc ctggagctgg cggaaaaagg ggaaaccccg caaaagacct tgcgtcttta
     3181 tcccatccag tgcgacaaca ccgtcacggg tttcaggacc cgcccggtca acccgcggga
     3241 attcaccgaa ctgaggaccc tcctgattca gcgaattgac gggaaaagcg cctccggaat
     3301 ctcggcaaag aaagacgcgc tgggctttca cctggagatc gccctgccct gaggccggga
     3361 agaagaaacc gcgggaaagg ggacttcaag catgcggaat tcccgtctca ccctgcctgt
     3421 cctggccgcc ctcggcgccg tctatttttt ctgggggacg acttacctgg gcatgaaatt
     3481 cgccatcgaa accctgccgc ccttcttcat gctgagcctc cgcttcacgg cagccgggac
     3541 gctcctgctc gcctgggccg caagccgggg caagcttgaa gccacgctgg ggcagcttcg
     3601 cggagcggcc cttgccggag gggtgatgct gctcggcgga acgggcctgg tcgcctgggc
     3661 ggagcagttc gtcccctcca acatcgcggc ggtcgtcatc gccacgatgc ccctctggat
     3721 ggccctggtc cggtgggtgg ccctgcgggg tgaccggccc gggatggcct ccctggcggg
     3781 actcttcctc ggcttcgggg gaaccctcct gctggtccgg tcgacaacag ccgcaggcgg
     3841 gacggaggag ggacacgccg ccggccttgc cctgctcgtt ctcgcggctt tcctgtgggc
     3901 atcgggatcg cttctttctc gtgcgctgga acagcccgcc tctcccggtt tcgccacctc
     3961 ggtccagctt ttttccgggg ggatctgctg cctcatggcg gggctcgtca cgggagaggc
     4021 cggacggatc cagatcgagg cggtttcttt ccggtctgtg gccgccatcg cctacctggc
     4081 cgttttcggg tccgtcgtgg ccttcagctg ctacacctgg cttctcaagg caacggaccc
     4141 cgccctggcc tcgagctacn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     4261 agctgctaca cctggcttct caaggcaacg gaccccgccc tggcctcgag ctacgcctac
     4321 gtcagcccgg tggttgcggt catcgcggga tggctgctcg ccggggagaa actgaacgcc
     4381 ggcagcctgg tcgcggcagg agtgatcctc gcctcggtcg ccctcatcat ccggggaacg
     4441 gccgggcccg gaaaggagaa cctggaaaga aagcctgcgg gaccgaaagg cttgccctga
     4501 tcctgcacgc ggtggtagga ttcgcgtgca ggcttttgac aatcgacacg cactgtctgc
     4561 gggggggccc ctggaagggg ctgagataaa gggtgttacc ctttgacccc acgaacctga
     4621 tccgggtcat gccggcgtag ggagcgaggc ggttttcttg gcaaagattg ttacggcgag
     4681 aagatacggc gggcttccct cggggagccc gccgattttt ttgggggagg tggaggcatg
     4741 tcgatctaca ggggagtcgc tttgaccgtc gccggaagcg attcgggcgg cggggcaggg
     4801 atccaggcgg atctcaagac attcgcggcg cttcgcgtgt tcggcacaag cgcactaaca
     4861 gccctgacgg cccagaacag cctgggggtt tccgcgatct gcggcattcc gcgggagatg
     4921 attcgggccc agatggaggc cgtttgcggc gatttcaagc cgaatgccgt caaaaccgga
     4981 atgctgggtt cgaaggaagc gatcctggag gtcgccggtt tcctcgccgg acgctgtccc
     5041 ttcctggtcg tcgacccggt catggtcgcc accagcgggg actccttgct cgagccggag
     5101 gcagaacgga ccctggggga aatccttgtc ccgaaggccg acctggtaac gcccaacgtc
     5161 ccggaggcgg aaaagctcac cgggcagccg gtccgggatc tcgacggcat gatcgaggcc
     5221 gccttctgca tcgcccggat gggagcccgt ggggtgctgg tcaagggcgg ccacatggag
     5281 ggtgctgtcc tgcgggacgt cctgctctgg gagggccgga tccggatttt cgaggagccg
     5341 aggatcccaa cgcagaatac ccacgggacg ggctgcaccc tgagcgctgc catcgcggcg
     5401 gaactggcag cggggtgcgg catggaggaa gccgtcctga gaggacgaag ctacatgaga
     5461 tccgcgttgg ccgccagttt tcgaggggga agggggcgcg gctgccttgg ccatgcggtg
     5521 agcgtcccct ggatcaagcc atgagtttcc ccggaaaacg ccttgaagac gcctggaagg
     5581 ctctccgaaa gcgccgcccc cttgtctacc acctgacgaa cggggtcgcc atggccgaac
     5641 aggcacacgt caccctggcc gcaggggcct cgccggtcat gtccctctgc gccgaggaga
     5701 gcgaatcctt cgcaagggca gcagacgcag tcctgatcaa tatcgggacc ccttctgcag
     5761 cttccgggga aatcggggaa agggtcgccc tcgccgcaaa agaagccggc cacccggtcg
     5821 ttctcgaccc ggtggggttc ggggccaccg accttcgaaa ccggctggtc cgttccctcc
     5881 tcaaaacccg ctgcatcacc gtcttaaagg ggaacgcctc ggagatctgt cttttggctg
     5941 gccaggccgg atccgttcga ggagtggacc gcggcctttc cccggacccg gcaacagccg
     6001 tgcttgaagt tgcccggcga gagaggctga ttgcggccgc caccgggaaa accgactggg
     6061 tcagtgacgg cgaacggacc tgggctgtcg aggggggaag cccgatgctc gaaagtttct
     6121 cgggaagcgg ctgctggctc ggatccgtgg tcgcagcctg tatcggcgct tccggcgatc
     6181 ctgtcgcggg caccctggcc gccctctgcg ccttcggtgc cgccgccgag gaggcagctt
     6241 ccctctccag ggggccgggg agttttcggg cccacttcct ggactccctt caccgattgg
     6301 cacttggcgg gacactcccg gtggccgggc gaattcggga ggtgagcccg tgagccccct
     6361 gtcctgcagg cttctcctta ccgtcctcgt ggacccggca ggactcccgg gaaggagcgt
     6421 tctcgaacag gctgaagggg ccatccgggg cggggcgaca gccatccagc ttcgctccag
     6481 cgaggcctcg gccagggagc tgacggcgct cgggaagtct ctcggggatc tctgccggaa
     6541 gttcgacgtt cttttcatcg tcaacgaccg gctggacgtg gccctggcct gcggctcgaa
     6601 cggcgttcac cttggccagg acgatcttcc cgtcgatgcc gccagggaaa tcgccccccg
     6661 gggtttcgtg atcggcgttt ccgctcacga cgcaaaagag gctcgtgaag cggaacggct
     6721 gggggccgat tatctcgggg tcggggccgt tttccccacc ttgaccaaga aaaacgcccg
     6781 caccattgga atcgccggat tccgggccgt cgtcgaatcc acgaccctgc cctgtgttgg
     6841 aatcggggga atcactgcag gcaccgccct ggaagtcctt cgctgcggag cctgcggagt
     6901 cgcggtccac tcggctgtcg ccaaggccga agacgccgag gcggccgcaa gggaattcag
     6961 ggttttcatg gaaatgggag ggcccaaccg tgcttgaggt tctggacttt tccctggaaa
     7021 ggggcggcgc acccatcctg gagggggcgt ccctgcatct tcaaaggggc ggatttggaa
     7081 tcctcgtcgg ccggtccggg tgcggaaaga cttctttttt taaggcactg gccggtcttc
     7141 tgcctcgcag ggcgggacaa atccggtgga ggggaaaaag cgttgaaaac ctggcgggtt
     7201 tggccgcctc catgcagcag aaagacctgc ttttgccctg ggcaaccctc gaggagaacg
     7261 tcttgcttcc agcgctggtc tcaggcaggg tcggtccaga agaaaggaac cgggcgaggg
     7321 agctcttgaa gcgatttggc ctcgagggat tcgaaaagtt cctgccgaac caggtttcgg
     7381 gcgggatgcg gcagcggtgc gccctggccc gaacggttct ttcagggagg gaaattctcc
     7441 tcctggacga accgttatcg gctctcgacg ctttgacccg ttcgcacctt cagcgagaaa
     7501 tcttcaggct gcagcaggaa ttcgggcgaa ccatcctgat ggtgacgcac gacgtggagg
     7561 aagcgctgct tttggccgac cggatcttcg tcttccacgg ggttcacgga ggaatcgagg
     7621 agctgctgaa cctctccggc acgaaaccca gggaaaccga cgctccggcc gtcgtgaaaa
     7681 accggaaggt tctgcttgaa gccttggaaa gaggccattc ccatgcagca tagagtgacc
     7741 gcgttcgccc tttcgctcct gtttcttgca gcgtgggaga ccctgtgccg tttttcggaa
     7801 atcccggcct tccttttgcc cgctccttca aggatcgctt ctaccctggc aggggacttc
     7861 ccgctccttc ttcttcacgg ggtcgcgacg ctgggagaaa tcctgctggg tgttgccggt
     7921 gcagtatccg cggcgatccc cctcgccctt gcgatgtttt caagcccctg cctcgaacgc
     7981 ggtctcacgc cgtttttggt ggcctcccag gccgtccccg tcttcgccct ggccccgatc
     8041 ctggttttct ggctcgggta cggcatagcg gggaaggtcc tggtggccgg gcttgtcatc
     8101 ttctttccca tcacggtggc cctgctggat ggactgaaat cctgcgatcc ggacctggaa
     8161 gacctgttcc ttctgatggg ggcctccttt cccgcccgcc tgcgcctgct ttatatccct
     8221 caggcccttc cctggttctt ttcagggctc cgggtcgggg tcaccgtttc cacgatcggc
     8281 gcggtcatcg gggaatgggt cggagcccag aaagggctgg gttacctgat gctccaggca
     8341 aacgcccggc tcaggaccga cctggttttc tcctgcgtgg cccttctcag tctcatcggg
     8401 ctttccctct ggttcggtct tggctggatc gaaacgaagc tgatcccctg gagggagtcc
     8461 ctgcaattca cccctaaggc aaggaggtct tgcaattgaa gagaatcctc tgttcgatcg
     8521 tcattctgct cgggctcgcg gcggcttgcc cggccgccca ggaagtcaag ctcatgctcg
     8581 actgggttcc caatgtcgac catcttccga tctacgtcgc gaaagagatg ggctttttcc
     8641 gaaaggaggg gcttgagatc cggatccttg caccctccga aacgacggat gccctgaaac
     8701 tggcagccgc ctcaaaggtg gaccttgccg tcggatatgc tccccaggtg atcgtgtcgg
     8761 ccgcggaagg cgttccgctg caggtagtag gacgcctgat ggagcatccc ttgagcaccc
     8821 tccttttcct ggaagaaaag gggatccagt ccccgtccga cctgtccggg aaacgaatcg
     8881 gctacacggt ccccggagtg atggacgttc tgctggacgc cttctcgaag ctgaacggcg
     8941 tgaagggaat cactcccgtc aacgtcgggt tttccatcgt gcagtctctc gcctccggga
     9001 aggtggacgc ggtgatgggc ggcttccgca actacgaagc cgttgaaatg attcagcagg
     9061 gaatgaaacc gtccgccttt ccccttgagc aatggggaat tccggattac gacgaactgg
     9121 tcttcgtgac ttcccggaat ttcgcaaaat caaacccgca ggtcatccga agcttccgga
     9181 tcgcgttgga caagggaatt cgcgcgtctc tcgcgaaccc gggaaaagcg cttgaaacct
     9241 atttccgggc agttcccgag gcgcctcgca atctcgagaa ggaagtcctt ggcaaaacgc
     9301 ttccgttttt cgcccgctcc caggagctcg acccttccag gtggaagagg ttttccgcct
     9361 ttgccctgag gtggggactt atcggacggg ccgtcgaagc agaacggctt ctctggaagg
     9421 gccggtgagg gcggtggcct gtacgaatct acagctccgc aagcttgtcc tggcggcgct
     9481 tttcgcgtcc gtcggcgtct tcctttccgg cctttcgatc cctctcgggc cgacccggtg
     9541 tttccccatc cagcacgccg tcaacgtcat cgccggtgtc cttttgggcc cctggtgggc
     9601 cgccggggcc gccttcatca ccagcacgct gcggctggcg acaggaaccg gaacgttttt
     9661 cgcctacccg ggaagcatcc cgggcgccct cgcggtcggc cttgcggcct ctcttttgag
     9721 gcggagacgg atctatgcct cgctggcgga acctctgggc acatccatcg ttggaacggg
     9781 cctgagcgcg gcggtgatcg cccccgctgt ccattccaac gccaccttcg ccatgctgtt
     9841 cgcgcccttt tttgccagca gcgtctccgg aaccgtcctg ggagccctcg tcctggtggt
     9901 tcttgagcga tccaggaggg caatcgaggc tgccgaagaa ggataaacct aaagaaaaga
     9961 ggcgggagct ccctgtcggg gcgctcaccg cctcttgctt tgccggaagg gttatttccc
    10021 gccccggtac agcttcctgt acggctcgaa gatcccgccc ctccaggagt caaagatcct
    10081 ccgctgccgg ctgtccagga ccgaacggat cctttccctc acccggtacg agaggaaaag
    10141 catgtccctc cgggcgttga gcaggtcccg cctgtagcgg gcctccgacc gggcggaaaa
    10201 gggcttccag ctgttcctga aatactcccg ttcaagtagc cagagacgcc tctcccggtt
    10261 tcgcatttct tccgccgcgt cccgaaggat ccggtcgatc tcccgcttct ggcgcggggt
    10321 caggtgaagc cgccgctcca gttccctcga aagctcccag cgaaccctgc cgaagtgcca
    10381 tccccggtct ccgtctcggt cgtaccatcg acccatccgt tcccacgcca ggcgccgctg
    10441 gtccggttcg aggatctggt agaaacggta gaggaagtca tcgtcggtcc gcaggaggaa
    10501 atcgaggtcc cgatccaggg aactctgtct tcgggaatcc aggacttccc cgacgatgat
    10561 cagcccaagg aggtcctcca ggtccgcgag acgctcagaa cggcgctcaa accgttcgtc
    10621 ccgccggaga tcccggatcg cctccctctg cctcggggtc aggcggaggc cgtcaaggaa
    10681 gtcgtccgag agaagccgtt caacgctgcg ccccgtccag tagaggtcgt cccggtcctc
    10741 cttccaggaa tccgcccggg cgggcaaagc agccgtcacg cacgttccag ccaggatcag
    10801 taccagcacg tttctccaga atcggttcat ctcgcaggcc tcctttgaga gtggaattta
    10861 aaggaagcgg gtcacctctt cggcggcccg cttccttcgg tcatttgtcg tttttcaccg
    10921 ggggcctcat catccaaggg gtctgggtac gctcttgcga gtcgagccat ctcctctggg
    10981 tttcgttcag gatcggccgg agcctttccc ggatctcgct ttccggcacc ctcagctgct
    11041 gccggtactc gaagagcctt ctccgctgct cttcccggtc acgcccggac cgatcgtccc
    11101 agctgtgcct gaagtaattc ttctccatct cccggtagcg cctctcccgg tccctgtatc
    11161 gatcgtccag gtcccggaaa acccgctcca tgtcgcgccg ctgccggttg tccagccgca
    11221 gccgcctgtc ccagtccctc gggagccagc ggtatccgtc gtccctccgg tcataccggc
    11281 ggtagatgta ccggtcatcg taccagcggt caaagcggag gatgaacgtt tgtctctggt
    11341 ccgcgtccag gatccgcatg aacaggtaaa ggaaatcgtc cagccgcatc atccggtaat
    11401 aatcggaatc ccagtagccg tcctgggact ggctgaaata caggcccagg gcaagaaggg
    11461 ccaggaacgt ccggaaattg tcgtcatctc ccctctcgat ccgaacgtcg taaccgtacc
    11521 cgcgccttcc gagatcgaac aggccgaaca gccggcccag atcccttccg tcccagcggt
    11581 cgtaccggct gtcgtaccgc cgctcatccc gccggttctc ccagcggtcg tcccgccgcc
    11641 catcgctgcg atcgtcccag tcacgccgcc ggagttcgtc gagcgaatcc cgctggttcc
    11701 gggtgaggtt cagggaattc aaaagttccg gtgtcaggag gctgtcgatc tcctcccgca
    11761 ccgagggcat gttggcaacc ggatcctgga actccgcggc ggcgtaggcc ggcagggcgg
    11821 cacccaggaa aaccgcggcc aacgcaacca tccacccgat tcctctccat ctcttcattt
    11881 tcaggacctc cattcctcgg aaaaagtctg gcattcctcc gagttgcggt cccattctag
    11941 gatttttttc cttaaggtgc ccttttgaaa cctttagaat tgtttaggaa cagcttcact
    12001 tgagcccgag ctccttcagt ttcgcctccg tggggattcc gttctcgtcc cagccgcgga
    12061 ccctgtagta ctccgccacc atctcctgga cacggctcac catgcccttt gctgcgcctt
    12121 cgggcaaggg ctcttccagg aggcgcttcg gaagggtgtc gtccttcttt ggatcgagcc
    12181 cttcccggag gttgaacagc cgctccaggt tccagatcct ctccccgcag cgcatcatct
    12241 cctccaccga gccgaccctc ccggttccgc tcttcagaaa ggccgcgtaa tcttccgccg
    12301 aaagggcgaa ggacgtgaac aggcacatgc ccgacgagtc gatggcggcg gtcaggtcat
    12361 tgaagatctt cgtccactcg gccttcccgt cgatcgtcca gggatccagt ttctgcggga
    12421 ttccgagaat ttcggcggaa atcatgtagc cccgcacgtg gcaggccccc cggttgctcg
    12481 tggcataggt cagcccgatt ccctggatgc cccgcgggtc ataggccggc agttcctgtt
    12541 ttttcacggt gatggaaagc tctggatgcc cgaaggcttc gcagagccgg gcgcttccct
    12601 ctgccaggag cttgcccagc ttgccctcgg ctttgcccat cttctgcagc cagtagagca
    12661 gggcttcccc ggaaccgaat ttcagctcgg ggcccccctc caggtcctcc ggcggcaagt
    12721 accccttctc gaacagctcc atggcggccg aaacggtacc tcccgcgctg atcgtgtcca
    12781 ggcccagctc gttgcagagg tagttggcca gggtgatgga ctccagttca acgaccccgc
    12841 agttggaccc gagcgaccag gtgctctcgt attccggccc ttcgccttcc ttttcgccca
    12901 ctttcgtgat ccgtccgcaa gcgatcggac aggcatagca ggcccgtttc ccggtcagca
    12961 gggtggccgc cagcgtttcc ccggattgcc ggtccgcctc ggccatggtg cccgattgga
    13021 agttccggta cgggtagctg cccgccgcgt tgatgatgtt gacgagcacc gccgttccgt
    13081 aggttggaag cccctggctc gtgaccccgt tttccttgat cttctgcatc cccacccgga
    13141 gggcttcctt cagcccctcc gggtctgcag cggtccaggc agcggacccc cggaccacca
    13201 cggctttcag gttcttcgat cccatgacgg cacccacgcc gcttctcccc atcgccctgg
    13261 cccggtcgtt gatgatgcag gcgatcctcg acaatcgctc tccagccggc ccgatgcagg
    13321 cgacctttgc cttttcgtca ccgacttctt ccaggaggcg gtccgtggtg aaggccgtgt
    13381 cctttcccca gaccgaaccg gcctcccgga tctctgtctc tccatcccgg atccagagat
    13441 agaccggttt ctcggctttt ccctccacca cgaggaactc gttcccggtc ctggcgaatt
    13501 ccgcccccca ctggcctccg ctgttgctgc aggcaatgag gcccgtcagg gggcttttgg
    13561 tcaccgccat ggacctcccc cccgtcggga cgttggaccc ggaaagcggc cctaacgcga
    13621 gaaccagctt gttttccggg gacaggggat cggttttcgc cggcacttcc tcgtaaagca
    13681 gtttcgtggc gtagccgcgg cccccgagga acgccttcgt ccactcctcg ttcaggggct
    13741 ctctcgaaat ggctcccgtc gcgaggttca ctctgagcag cattctttct gccatggatc
    13801 ctccctccgt ctctttttgg gcttgctgtc cttttctctc gcactgattt cccgcgtgac
    13861 cgaacggatc ttttcctcgg gatcccgctt ttcttccctc ccttccgttc aggggcaccg
    13921 gatcaggttc cggtcccaca ggcagtcgct gcaggccggg gagtcccccc agcagtcgct
    13981 ttccgtggtt ttcgggtatt cacagaccgg cagaaggttg cagtccaggc atgaggcgta
    14041 aagcgcccgc cgaagccgcc accggaaact cgtgtaggcc gggctgtccc agatcgcctc
    14101 aaggctttcc tccgccaccg aaccgaaaga atgggcacac acccgtttct tccgtcccaa
    14161 gacccattcg tcgtagccgt gggacagccg aatgcaggga ctgacatgtc catcccaccg
    14221 gaccagcagg gtttccgatc gcgcgaacgc gcaatgccgt tccgaggaaa gggcaaaccg
    14281 gggcagtgca acgttcagcc cctccttctg aaaagcaagg aagagttttt tctggagaac
    14341 cgcctcttcc tccagggaaa ccgggtaaag ggcaagggaa cgcattcctt ccgaggtcgg
    14401 gacgaggttg ctcagcgaca cccccctggc cccggcctcc cggagggccc gaggcagggt
    14461 gagaaccttt tccaggttcg aagccgtaag cacgatcgcg gcccggattt ccgggaaagg
    14521 gctgcctgcc gcctttcgag cctcctccag ggtccgcatc gcctccagga cggtccctgg
    14581 gtccgaatcc cttcccggaa gcccgaccgc ctccgcctcc agggaaaaat cgacgatcct
    14641 gaggccggcc cggaccaggg cgcgtgccgc atcaacatcc acgagggtcc cgttggtcgt
    14701 gaggcggagg ctccatccct tttccgcggc aagctgaaac gcctcgatcg cacgagggtg
    14761 aaccaggggt tcgccgatcc ctccgaaatg gacccattcc aggttcggaa accgccttgc
    14821 ctgttcgaag agatccaaga gaaccttcca ctccaggtcc ccccgggttt ccccggaacc
    14881 tttgaggaaa cacatcgggc aatccaggtt gcagcgggag gtcaattcaa ggcggatctt
    14941 ccggagcctg cgttccctcg gaatggaaac ggtgctgtcg tcaaagcgca gcagggtttc
    15001 gccggctgaa acgttcacgg cccagatttc ctttcatcat ttaagaagtt tgagcgaagg
    15061 gcaggaaaag cgttgcgaaa gaggggtggg aaaggtatga ttttcttgta tcgcgctatc
    15121 gtattatagg gcttttgtgg actgaaatga agtgttagag tgtggatgga gtgaaatcaa
    15181 atgacccgta ccagtatccg gaggcgaggg aaaattcgcc tccgcaaaaa cctgaactgg
    15241 cggatctggg agcttctccg ccgcgggatg aagcaatgga ggaacagggg gatcgtgccg
    15301 cggccccagc cgggcctcaa cccctgcgaa acgaacggga ccggaaaact ttgagggagg
    15361 gtgaaaggct tggaacagaa cttcaaggac atcctcgaca acatgctcga agcggtctat
    15421 gtcaccgacc gggaccgtcg gatcgtctac tggaaccgga gggccgagga cctcaccggt
    15481 ttcatgaagg aagaggtcat cggccgtgtc tgctcggaaa acatcctcgg acacgttgac
    15541 cgcaggggga ttcccctctg caagggggtc tgtccccttt cggaaacgat ggcggacggc
    15601 aagcccaggg aaacgatggt tttcctgcac cacaaggagg gtttccgaag gcccgtccgc
    15661 gtccggacgt cccccctgat agactcgagc ggcaacatca cgggggggat cgaggtcttc
    15721 gaggatgcca tgggatacct ggccctggag gaaaaaatcc agcttctgga atcgctttcc
    15781 ctcctggatc cgctcacccg tatcggcaac cgccgctacc tggaggaaca gctggaagaa
    15841 aagttcaagg agttccagat ctccggtctt tccctcggcc ttctcttttt cgacatcgac
    15901 cacttcaagc gcgtgaatga cacctgggga catgttgttg gcgacagggt gctgaccgtc
    15961 gtcgcctcaa cgctcaagca cagcctgagg atctacgatg ttctcgggcg ctggggtgga
    16021 gaggaattcc tcctccttct cccgaacctc agggaaggcg ctctggaaga agtctcagac
    16081 cggcttcgcc agatggtctc ggcttcggac atcttcctcg agaacagtgc cgaacccctt
    16141 catgtcaccg tgtcgggcgg agccaccctg gcccgtctcg gggaaacccc tcagaaagcc
    16201 cttgaacggg ccgatgcgct cctgtacgag agcaaggccg cagggcgaaa ctgcatcact
    16261 ttcggataga acccaagggg gggcgaaagc cccccttcct ttttctgcca gggagcacac
    16321 ccccggctca acaccggtca tccagcatgc cgccgaggtt ggcctggagg gccgttttgg
    16381 cgcagagccc gaggatgccc cgtttcacct tcggctccgg ctgtttccat tgggtcagcc
    16441 tcttcaagat gacgggctcc gggagatcga ggttcacggt tcgtccctcg atgtcgatgg
    16501 caatcgggtc gccttcctcg atgaccgcga tggggccccc gacaaaggcc tccggggcca
    16561 cgtgcccgat ggcgcagcct tcggtgaacc ccgaaaagcg cccgtccgtc aggacgtaaa
    16621 cgtccgaacc gagcccgagc cccaccaggg catcggtcgt catcatcatc tccctcatgc
    16681 ccggtgctcc cctcggcccc tccatgcgga tgaccacgac gtctcccctg ttgatgtctc
    16741 cccggacaac ggcctcgtgg gcagcatcgt ccgaggagaa aacccgggct ggaccccgga
    16801 agcgcatccg gtccgccggg atggtggtcg tccggcaaat ggcgccgtcg ggtgccaggt
    16861 tgcctctcag gacggcgatc cctcccgact tgaaaacggg atcgccgaca ccggccagcg
    16921 tcgtcggatc cagggaacct ttccggggca ggacctcccc caggggcttt cccgtcaccg
    16981 tcatggcctc gaggtcgaga ctcttttcca gttccttcag cagggccggg acgccgcccg
    17041 cctcgtgcag gtcgacgacc gtctttttgc ccgtggggat caccctcgag agaaccggga
    17101 tctctcggga aagacggtcg aaatcgtcca gcgaaaggtc gatgtccagc tcccgggcga
    17161 tgctgaccag gtgcaaaacg gcgttcgtgg aaccggccag cgccagggtc gccttcactg
    17221 cgttcaggag gttgctgcgg gacaggacct tgtccacgga aaggccttcc ttcaccatct
    17281 cgacgatccg tcggccagtc ctgcgcccca ggcgaagctt ctcggcataa acggccggaa
    17341 ccgtcgccga acccggcagg gagagcccca gcacctcggc caggatctgc atcgtgttgg
    17401 ccgttcccat cagggagcag gcccccgggc cgggacagac gtgatcctcc aggaagcgca
    17461 tcctctcgga aacgtcctcc tttccgtacc gcgccccgaa gacgacctgg tccagctccg
    17521 acatgacgac ctgtcttcct tcgagacggg ccgaaagctg cggtcccccc gagaggaaga
    17581 tgaagggaag tcccgagcgg atccctccga tgatcacccc ggggatgatg ctgtcgcacg
    17641 aggccaaaag cacgagcccg tccaggagct gcacgttcgc catgatctcg atcgaggacg
    17701 cgatcacgtc ccggatgacc agttcgtacc gcatgtgggg cgtcccgatg ggga
//
LOCUS       DAOOVN010000022        17052 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_2285_length_17052_cov_12.301641, whole genome shotgun
            sequence.
ACCESSION   DAOOVN010000022 DAOOVN010000000
VERSION     DAOOVN010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 17052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17052
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2285_length_17052_cov_12.301641"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="PLY83_03520"
     CDS             complement(<1..422)
                     /locus_tag="PLY83_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HOV29139.1"
                     /translation="MVEETESYRCRCIVKGSGSGEALVSREPMCFYLCDPLTGTVIEK
                     NHPLQGRSLAGKVLVLQSGKGSSVVQVDGFYQLWVKGNLPAAIIVAEAEPVLVSSAVV
                     VDAVMVDRLEVNPFEAIEDGDWVEVDSTEEIVKVTKRRP"
     gene            complement(422..1639)
                     /locus_tag="PLY83_03525"
     CDS             complement(422..1639)
                     /locus_tag="PLY83_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HOV29140.1"
                     /translation="MFLSDEEKRMLDGEMGQGVRKAMELLVALGKTFDAERLIPITRA
                     HVALSGQEGDTYWCGLLADGGARCKVPPTTNPAWDVTNLSKRFEVTDSELDLALRTIE
                     VYRKIGAQLTFCCTPGLSTNVPAFGEHVAFSESSATPYVNAVLGARSNRESSVSALAA
                     AVVGKTPLYGLHLDENRRGQFLVEVEATLEEPYDWGLLGWLVGRFSGSRVPVFNFRTL
                     ARRPSPEALLYLGAELNTSGAVPMYHIAGLTPEAPTLQAAFGSSPPEAELVVTEEDLR
                     NQEEAISEQGGPINLVMLGCPHYSYGQIREIERLMEGRRVREGVAFWVLTSFEALELA
                     TRSGEKERLAALGVDLVADTCIDEPCWKSFEGGLGVTDSPKCAYYRERRGQPFVIRSL
                     EKCIEAAILGEVR"
     gene            complement(1651..2943)
                     /gene="larA"
                     /locus_tag="PLY83_03530"
     CDS             complement(1651..2943)
                     /gene="larA"
                     /locus_tag="PLY83_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HOV29141.1"
                     /translation="MKQSIPYGTSEQTFELPRENLIFQGKMPHVRPLRNLEEAVLESL
                     DSPIGTPPLSTLAEGKKNIVFLIEDNTRNTPLKTLLPILVDYLKRHGVPDSAIRFLTA
                     PGTHRLMTQEEILEKIGPEMARRFQVFQHDATRMEDLVDLGSVMAGNYRVPVQVNRKA
                     LEADLLIGLGDIVPHCDAGYSGGAKIIQPGICGFATTAATHVAAALLAEIPLGVMENP
                     CRHGMEEVARKVGLDFILNTVRNEQGQVVGIFSGDFVKAHREGAALARTTYRVDVPGP
                     ADIVVVSSSPCDIDFWQAGKGLISAYFAVRKGGILVFAAPCREGLAHNHPRFRDWLAL
                     PLNEILKRANACSPEDRDADLISADLAACNSRVRERASVFIVTEGLSGEEIQTLGFER
                     FDSVQAALDEAFARIPGGTVGILPRGGVALPVVGETHA"
     gene            complement(2940..4388)
                     /locus_tag="PLY83_03535"
     CDS             complement(2940..4388)
                     /locus_tag="PLY83_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOV29142.1"
                     /translation="MSRSEYSEVTEEVLRDLENILGPSNVSVNPEKRQAYSRDEVPPQ
                     QWGDLPPAEVLCFPETTEQVSRLLSYADQHRIPVTPRGAGTGLSGGAVPSHGGILLSF
                     EKMNRILELDSANLTMTVEPGVITSEITKAAQSAGLLYAGDPCSGDISTIGGNVAENA
                     GGNKVVKYGATGNHVLGLEVVLPDGSICQFGGKRRKDVTGYDLVHLIVGSEGTLAVVT
                     KIILKLFPLPRKVVDLLIPFHDTASAIALVPRLIIDGKVMPSTIEFMDKSSLQLVKKY
                     LNLRIPCEEAGAHLIVQLEGNDPDWLKSEILRVGDLCRECGALEVFVAESKTHRNIIW
                     KLRKGIAEEPWAHDMPCHLEEDIVAPIGEIPSLMDFLRDLEANGAIRYIAYGHVGDGN
                     MHITLFTEKGAPDSGNVLENARLELYRKVSERGGTLTGEHGVGLKRRHYLPLFLDEAQ
                     IRLLRRIKMAFDPHNILNPGKITPPAEEEGLG"
     gene            complement(4375..5400)
                     /locus_tag="PLY83_03540"
     CDS             complement(4375..5400)
                     /locus_tag="PLY83_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOV29143.1"
                     /translation="MNVQSKNVMIFGEVRTNGRLHPATLELTGKGRELADALSDSAGV
                     SVVLCCGKLEDDVLLPVHYGADEVILVEHPSLSFPNPEVAVKILVTLVKERQPEILLA
                     PATPQGRCVFPALAAKCRTGLTADCTGLEIERGTGLLLQTRPAIGGNVIATIKTPNHR
                     PQMATVRPKTFAVPEPRPRKGEVVRPSLPQESFQSLTENLDFTPFQDKQAPVRDAEVV
                     ISGGKGLRRAEGFGLLRELADLLGGSVGATRPTVEAKWIPYAHQVGLSGQVVSPKVYL
                     AAGISGAVQHLAGMQTSRKIVSVNKDPEAPIHKIADIALCGDLYEILPQLIQRIREER
                     GTLDVEI"
     gene            complement(5413..6201)
                     /locus_tag="PLY83_03545"
     CDS             complement(5413..6201)
                     /locus_tag="PLY83_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOV29144.1"
                     /translation="MKIAVLAKQVPDTDEVKMDPETGTMIREGVDRTVNPLDLHALEA
                     ALCLAKNHGGSVTVFSMGPPQAEEALRECLSLGADDAVLISDRAFSGADSWTTAVTLA
                     AALRLSGPFDLILAGDKATDGETGQVGPEVAAILEIPFATHVIDLACPDERHLVVSRN
                     LDEGIQKQRLPLPCLLSVLSDLNEIQLPTLKGFRNAWKAAIRTLSREELGLPREAVGL
                     AGSPTRVVRIDHPRFTRQTEKYSGTEVARGVERVVEILRESALL"
     gene            6349..7023
                     /locus_tag="PLY83_03550"
     CDS             6349..7023
                     /locus_tag="PLY83_03550"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOV29145.1"
                     /translation="MTEQAQSQGRRSRKAAGWVAEEIRRMILENTLCPGELINECRIG
                     EKLGVSRTPVREAIGQLAQEGLLKRIPGRAHLVADMSLDDIKEINDLRMVLEPLAGRE
                     AVGRIPGEKIQSALREWKNFRDLFERGNPILSREISEADSRLHGMIIDYCRNSRLKTF
                     LSILRYQIFRCLVASWEVRDFVADSIDQHIEILELLEKKDADGLEAALRSHILFNNKV
                     YLQKAL"
     gene            7133..8377
                     /locus_tag="PLY83_03555"
     CDS             7133..8377
                     /locus_tag="PLY83_03555"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOV29146.1"
                     /translation="MEKGFDFREQLSVMARERLESSSIGEIIELAARHRALSFGAGEP
                     SAELFPKNAIREAVAEAFAEKDVWGYYHDDFGYLPLREWICERMGKDGMAPSWVQPEH
                     ILLTNGGGEGVSLVSEALIDPGSVVLVESPTYVESLLTFRKQGAVCVPVPSDDEGIIP
                     SELERIASAVRPRFLYTIPNFQNPSGRTIPEERRVQILEILRKLDFPMLEDDPYHYLS
                     YDAVPPTSFLKLTGNDYRVVHCNSFSKTIAPGLRVGWLVVPPPLKKALHHLRVSAGLG
                     RPTVIQAGIWRYLSGIDFLSRIEELCKNYRERRDAMMDGIKRFLNPLGIRASQPAGGF
                     FIWCESPDSRDIHSFARYSVEEHGIAIIPGQSFFPKGDKRGDWAFRLSYAKVTAEQVE
                     EGMQRLVRAWESYPAARATSGF"
     gene            8418..9416
                     /locus_tag="PLY83_03560"
     CDS             8418..9416
                     /locus_tag="PLY83_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HOV29147.1"
                     /translation="MSKRFLIGCLLALCMAAFALPAFAAAPIVIKVAAVTSDNHPDFI
                     AMRDVFKKNLEARSGGRIKVELYPNGQLGGDRELIESVQMGSLEIAMPASSVVAGFDP
                     RFQVLDLPFLFKDKKTAFAALDGELGKKLNDGIVSKGFIVLGFTENGFRHVSNNRGPV
                     TKPADLKGLKIRTMENPVHVAFFKLLGANPTPINYGELYTALQQKTVDAMECPAVLYE
                     DGKFYEVQKYLSLTGHVFSVVPVMIGKKFFDGLPKDLQKMVKEEARRYVLAQRKIIDA
                     EEKKIFDVLKKKGMVINDLTPAQKKLFVQATAPAYEQFRGKIGSDLVDMAIKIANQ"
     gene            9513..10007
                     /locus_tag="PLY83_03565"
     CDS             9513..10007
                     /locus_tag="PLY83_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOV29148.1"
                     /translation="MWERISKTLNDVEEWFLVVTIAANVLLVFYQVIMRYVFQNSLSW
                     SEELARYLFLWYSWVGTSYAVRFRGHLRVEMFANMLKGRTRKWFELLVIGIWFLFSLF
                     LAYQGVKVTRFIAESQQLSSAMQIPMAWAYASVPVGCVLMSLRLVGDLWDIWKKLGCE
                     EEPA"
     gene            10010..11290
                     /locus_tag="PLY83_03570"
     CDS             10010..11290
                     /locus_tag="PLY83_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOV29149.1"
                     /translation="MEATLLFTTLFILIGISVPIGITLGLASAICFYFTTNIPMTFLA
                     QKSFTGLDAFPFLAIPFFILAGSLMCSGGISRRLVNLAESLVGFIAGGLAMVTVLACM
                     FFAAISGSGPATVSAIGSFMIPAMKERKYDGSFAAAITAAAGTIGVIIPPSIPFVIYC
                     VVAQVSIGDMFIAGVVPGIIIGISLITLSYFIAKKKGYVASAETLSFRKAAHAFREAI
                     WALFVPVIILGGIYGGIFTPTEAAVVAVIYSILIGKYVYKELDNRQIVECLKSTALIN
                     GALEYLIGFSMAFAQYLSLAHIPAAIGDWLTSFSSNPYVILTLINILLLVLGCFLDNI
                     AAVLILTPILLPVVKSLGVDPIHFGLIISFNLAIGFITPPYGINLFVASTISGENIES
                     ISRSILPVIATLVGCLMLFTYFPVFSTGLVHLLR"
     gene            complement(11375..12178)
                     /locus_tag="PLY83_03575"
     CDS             complement(11375..12178)
                     /locus_tag="PLY83_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HOV29150.1"
                     /translation="MSLTMEDLAHVSPKEAREAIRAGKWPRPTSGLCRGHVQANLVIL
                     PKDWAYDFLVFAQRNPKPCPVLDVTEPGDPEPRIVAPGADLRTDLPRYRVWKDGKLAA
                     EPEEITEYWRDDLVAFLLGCSFSFEGALLEAGVPVRHIELGLNVPMYQTNIPCRPAGR
                     LSGPMVVSMRPIPHSLVPKAVLCTGRFPAVHGAPVHIGDPAVIGIADVNHPDFGQAVP
                     IHEGEVPVFWACGVTPQAALMASKPPFAITHAPGHMFLCDPKDSDYAVF"
     gene            complement(12175..12948)
                     /locus_tag="PLY83_03580"
     CDS             complement(12175..12948)
                     /locus_tag="PLY83_03580"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HOV29151.1"
                     /translation="MAKIDLNSDLGESFGPWKMGMDREVLAHVSSANVACGFHAGDPS
                     VMVKTVKSARDAGVAVGAHPGYPDLVGFGRRNMACSPDEVYADCLVQIGSLLAVCRAA
                     GVRLQHVKPHGALYNQAAKSEALAEAVAFAVKDAGEGLVLLGLANSLFEPAAAKAGIP
                     FAAEAFADRSYQSDGSLTPRSQPGAVIHDPRLAAARVVQMATEGVVETADGKKIHFRP
                     HSICLHGDNPEAVAMAAAVRAALEEAGVTVANLREVLSA"
     gene            complement(12969..14033)
                     /locus_tag="PLY83_03585"
     CDS             complement(12969..14033)
                     /locus_tag="PLY83_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HOV29152.1"
                     /translation="MGLFLNVKKGGMSTTVQDFGRWGHQAMGMPVAGAMDPEALAVAN
                     LLAGNSPGEAALEITLLGPTLEVEGEGTVAASGADLGLTINGTPAPLWTCLSVKTGDV
                     ISFTGPKGRGCRAYLAFSGGIDVPVVMGSRSTYTRAGIGGFEGRALKAGDRVSCGEPL
                     PLWKRTIGFSCPESLRPVRDPEAPIRAVLGPQDDAFTPEALETLFGSTYTITSQADRM
                     GYRLEGPKIPHRTGADIVSDAIPLGAIQVPGHGQPIVMLADRQTTGGYTKPAVVLTAD
                     LAVLAQRIPGETVRFARSTPGEGAKLLAERAKRLAALGQLRSEYRTRPQAVGGEVKAT
                     SGAFTLRIGGKTALIEWQEL"
     gene            complement(14035..14778)
                     /gene="pxpB"
                     /locus_tag="PLY83_03590"
     CDS             complement(14035..14778)
                     /gene="pxpB"
                     /locus_tag="PLY83_03590"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HOV29153.1"
                     /translation="MEEPRTPRLLDAGDSCLVAEFGASVDRALNAKVQALKHALAKCA
                     VKGIVELVPTYRSLAVYYDPLLLDREELKKRIESACETLDEASQAESRVVTIPVCYGG
                     TFGPDLENVCAYTGLSKEEVIARHTGRDTYCFMLGFTPGFAYLGGMDESLATPRLENP
                     RTLIPAGSVGIAGKQTGIYPIDSPGGWQLIGRTPLAVFDPLRTPPTLVEAGDWVRFEA
                     ISESEYEAIRAAAKAGQYTVKIALRKEAA"
     gene            complement(14839..15978)
                     /locus_tag="PLY83_03595"
     CDS             complement(14839..15978)
                     /locus_tag="PLY83_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HOV29154.1"
                     /translation="MATSAIGPGFLTQTAVFTDKLKAAFAFAILASVLIDVIVQLNIW
                     RILGVSGMRAQDVANKVIPGLGYFLAFAVALGGLVFNIGNIAGAALGVNVLFGSNMAV
                     GAIVSAALAIFIFLNKEMGKTMDTFTKVLGFLMIALVLFMVFKTQPPVGLALKESVLP
                     STIDWMMVLTLVGGTVGGYITFSGAHRIIDAGITGQDKVKQISYGSVQGIAITGIMRF
                     LLFLAILGVVAMGKPLDPSNPPASAFQIGAGMIGYKIFGVVLWAAAVTSVVGASYTSI
                     SFLQTLFKNVKQYQRWWIIGFIVASTTIFTTVGKPVNLLIFAGSINGLILPLSLLSML
                     LAAHRKDIVGDYRHPKILTALGFVMVAFTAWMGWQSLSKIVELIK"
     gene            complement(16282..>17052)
                     /locus_tag="PLY83_03600"
     CDS             complement(16282..>17052)
                     /locus_tag="PLY83_03600"
                     /inference="COORDINATES: protein motif:HMM:NF016439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29155.1"
                     /translation="DLPHCLRLQLSRQGLEGSDAWILLEEGLEELARQDLAALRKKLA
                     WERQRLDAALRSLRRLDPHPGFNFSHPGTVLPEVEIRYDERGRPTVRLLHENLPRVLL
                     DAELYAAAGDVARGPFREARGLLSALAARYRTKMRLALLLAERQQAYLRNLASAPVPL
                     TLAPAGQKLGLSASTVQRAAACTWAVTPRGTLPLSSLLGRGIAARPDLSTRALREAIR
                     SGWESGKPDSVLARELGLPPRTVTWHRQRLGLPPVRRA"
BASE COUNT         3292 a         5020 c         5190 g         3550 t
ORIGIN      
        1 ggtcttcttt tcgtgacctt aacgatttct tcggtagagt ccacttcaac ccagtcgccg
       61 tcttcaatgg cctcgaaggg attgacttca agcctgtcaa ccatgacggc atcaacgacg
      121 accgcggagg aaacgaggac gggttccgct tccgcgacga tgatggccgc ggggaggttc
      181 cctttcaccc acaactggta aaacccgtcc acctggacca cggagctgcc cttgccggat
      241 tgcagaacca ggacctttcc cgcaaggctc cttccctgga gcgggtggtt tttctcgatc
      301 accgttccgg taagcggatc gcaaagatag aaacacatgg gttctctcga gacgagagcc
      361 tcccctgaac cggatccttt cacgatgcag cggcaccggt acgattccgt ttcctcgacc
      421 atcatcgaac ctccccaagg attgcggcct caatgcattt ctcaagactt cgaatcacga
      481 aaggttgtcc ccttctttcc cggtaatagg cgcatttcgg agaatcggtc acgccgaggc
      541 ctccttcgaa agacttccag cagggttcgt cgatgcaggt gtcggcgacc aggtcaacgc
      601 cgagagcggc aagccgttct ttttctccgg agcgcgtagc cagttccagc gcttcaaaag
      661 aagtcaggac ccagaaagcc actccttccc gaactcgccg cccctccatg agtcgttcga
      721 tttcgcggat ttggccgtag ctgtaatgag ggcatccgag catgacgagg ttgatgggac
      781 ccccttgttc ggagatggct tcttcctggt tcctgaggtc ttcctctgtg accaccagct
      841 cggcttcggg cggggaactc ccgaaggccg cctgcaaagt cggggcttcc ggtgtcaacc
      901 ccgcgatgtg gtacatcgga acggcaccgc tcgtgttcag ttcggctccg aggtagagaa
      961 gggcttcagg cgaaggcctc cgggccaggg tcctaaaatt gaaaacaggg acacggctcc
     1021 cggagaagcg cccgacaagc caaccgagca atccccagtc gtagggctct tcaagggtgg
     1081 cttcgacttc aaccaggaat tgcccccttc ggttctcgtc caggtgcagg ccgtatagcg
     1141 gtgtcttccc cacgacggca gcagccaggg cgctaaccga tgattcccgg ttgctccggg
     1201 ctcccaggac tgcgttgacg taaggagtcg cgctggattc cgagaaagcg acatgttccc
     1261 cgaaggcggg aacgttggtg gagagcccag gggtgcagca aaaagtcagc tgagctccga
     1321 ttttccgata aacctcgatt gtcctgaggg caagatccag ttccgaatcg gtgacttcga
     1381 agcgcttcga aagattcgtg acatcccacg cagggttcgt ggtgggggga accttgcagc
     1441 gggcgcctcc gtccgccaaa agcccgcacc agtacgtgtc cccttcctgt ccgctaaggg
     1501 cgacgtgcgc ccttgtgatg gggatcagcc gctccgcgtc gaacgtcttg ccaagggcga
     1561 ccaagagctc catcgctttc ctgacgccct gccccatctc accgtcaagc atcctttttt
     1621 cttcatccga tagaaacatc ctttccctcc ttatgcgtgt gtttctccga ccactggaag
     1681 agcgacgcca ccccttggga ggattccgac ggttccgccc ggaatccggg caaaggcttc
     1741 atccagggcc gcctggaccg aatcaaagcg ctcgaatccc agggtctgga tctcttcccc
     1801 ggaaaggccc tcggtgacga tgaaaacgct cgctctttcc ctgacgcgag agttgcaggc
     1861 cgcgaggtcc gcagaaatca ggtccgcatc ccggtcttcc ggggaacagg catttgccct
     1921 cttcaaaatt tcattcaacg gaagtgcaag ccagtccctg aatcttgggt ggttgtgcgc
     1981 aagcccttcc cggcatggag cggcaaagac gagaattccg cctttgcgaa ccgcgaagta
     2041 ggctgaaatg agccctttcc ctgcctgcca aaaatcaatg tcacaggggg aagagctgac
     2101 aacgacgatg tctgcgggcc cgggaacatc gacacggtag gtcgttcgag ccagggcggc
     2161 tccttccctg tgcgctttca cgaaatcgcc gctgaagatt cccacaacct gtccctgttc
     2221 gtttcgcacg gtgttcagaa taaagtcgag cccgactttt cttgccactt cctccatgcc
     2281 atgacggcac gggttttcca ttaccccgag gggaatttca gcgagaaggg ccgccgccac
     2341 atgagttgcg gctgtcgtgg cgaaaccgca aatccctggc tggataatct tagcgcctcc
     2401 ggagtacccc gcgtcgcaat gggggacgat gtcaccgagc ccgatcagca ggtcggcctc
     2461 cagcgccttg cggttcacct gcacgggaac ccggtagttg cctgccatga cggatccgag
     2521 gtcgaccagg tcctccattc gggtggcgtc atgctggaaa acctggaaac gcctggccat
     2581 ttccgggccg atcttttcga ggatctcttc ctgtgtcatc aggcgatggg ttcccggggc
     2641 cgtcaggaag cgtatggcag aatcagggac cccatgacgt ttcaggtagt cgaccaggat
     2701 gggcagaagg gtcttcaggg gggtgtttct cgtgttgtct tcgatcagga agacgatgtt
     2761 cttcttccct tcggcgagcg tcgagagcgg aggcgtgccg atcggtgaat cgaggctttc
     2821 aaggaccgct tcctcgagat tccgcagagg gcggacatgg ggcatcttcc cttggaaaat
     2881 caggttttcg cgggggagtt caaacgtttg ttctgaagtt ccataaggga tggattgctt
     2941 catccgagtc cctcctcttc tgcaggggga gtgatttttc cggggttaag gatgttgtgc
     3001 ggatcgaacg ccattttgat gcgtctgaga aggcggattt gggcttcatc aaggaaaagg
     3061 gggagatagt gccttcgctt caggccgaca ccatgctcac cggtaagggt accgccccgt
     3121 tcgctgacct tcctgtaaag ctccaggcgg gcgttttcaa gaacgttgcc tgaatccgga
     3181 gccccttttt ccgtgaagag ggtgatgtgc atgttcccgt caccgacatg accgtaggcg
     3241 atgtagcgga tcgccccgtt tgcctccagg tccctcaaaa aatccatcag agaagggatt
     3301 tctccaattg gagccacgat gtcttcttca aggtggcagg gcatgtcatg ggcccagggt
     3361 tcttctgcaa tccccttccg gagtttccag atgatgtttc ggtgggtttt gctctccgcc
     3421 acgaaaacct ccagggctcc gcattccctg cagagatctc ccacccgcag gatttccgac
     3481 ttcaaccaat cggggtcgtt tccctccagt tgcacgatga gatgggctcc cgcttcctcg
     3541 catggaatcc ggagatttaa gtactttttc acaagttgga gagagctttt gtccatgaac
     3601 tcgatcgtgc tcggcatcac ctttccgtcg atgatcagcc ttgggacaag ggcgattgcg
     3661 gatgcggtgt cgtgaaaggg gatcagcaga tcgaccactt tcctggggag ggggaacagt
     3721 ttgaggatga ttttcgtcac cacggcgaga gtcccctctg acccaacaat caggtgaacg
     3781 aggtcgtagc ccgtaacatc cttacgcctc tttcctccga actggcaaat cgagccgtcc
     3841 ggaaggacga cctcaagacc caggacatga ttgccggtag cgccgtattt gacgaccttg
     3901 ttcccgcccg cgttttccgc gacgttccct ccaatcgtgg agatgtcgcc actacagggg
     3961 tccccggcgt acaaaagccc ggcagactgg gctgccttcg tgatctcgga agtgatgacg
     4021 ccgggttcca cggtcatcgt aaggttggcg ctgtccaatt caagtatgcg gttcatcttt
     4081 tcgaaggaaa gcaggatgcc cccgtggctg gggacggcgc caccggaaag ccccgtcccg
     4141 gccccgcggg gggtaacggg aatgcggtgt tggtctgcat aggaaagcag cctcgacacc
     4201 tgctcggtgg tttcaggaaa acaaaggact tccgctggag gcaggtcccc ccattgctgg
     4261 ggaggaacct cgtccctgga ataggcctgg cgtttttcag ggtttacgga cacgttggaa
     4321 ggtccaagaa tgttttccag gtcgcgaagg acttcttcgg tcacttcgga atactcagat
     4381 ctcgacatcg agagttcccc tttcttcccg gatccgctgg atcagttggg gcagaatctc
     4441 gtagaggtct ccgcaaagag cgatatctgc gattttgtga atcggggcct ccgggtcctt
     4501 attcaccgac acgatctttc gcgaggtctg catcccggcg aggtgttgca ctgcaccgga
     4561 gatccccgcg gccaggtaga ctttcgggga gaccacctgt cccgacagtc caacctggtg
     4621 ggcgtatgga atccattttg cctccacggt ggggcgtgtg gctcccacgc tgccgccaag
     4681 caggtccgcc aactcgcgta gcaggccgaa cccttccgct cttcttagcc ccttccctcc
     4741 cgagatcacc acttcggcat cgcggacggg agcctgctta tcctggaaag gggtgaaatc
     4801 caggttttcc gtcaaagact gaaaggattc ctgcggcaag gaggggcgga cgacctcgcc
     4861 cttcctgggg cgtggttcgg ggacggcaaa tgttttcggc cggacggtgg ccatctgggg
     4921 gcggtgattg ggggtcttga tcgtggcgat cacgttcccc ccgatggcgg gcctggtttg
     4981 aagcaaaagg cccgttcccc tttcgatttc cagtcccgtg caatcggccg tcaaccctgt
     5041 tcggcatttt gccgcgagcg cggggaaaac gcatcttccc tggggagtgg caggagcaag
     5101 caggatttcc ggttgcctct cctttacgag ggtgaccaaa atctttacag cgacctcggg
     5161 gtttgggaaa gagagcgaag ggtgttccac caggatgact tcgtctgcac cgtagtggac
     5221 cgggagaagg acgtcatctt ccagcttccc gcagcacagg acgacggaaa ccccggcgct
     5281 gtcgcttagc gcatcggcca attcccgccc tttccccgtc aattcaaggg ttgcgggatg
     5341 gagcctgccg ttcgttcgaa cttccccgaa gatcatgaca tttttggatt ggacattcat
     5401 cgcgattcgc tcctagagca gcgccgattc ccgcaaaatt tcaaccaccc gctccactcc
     5461 ccgggcaact tcggtgcctg agtatttctc tgtctggcgc gtgaaacggg ggtgatcgat
     5521 ccgaacgacg cgtgtcggcg aacccgccag tcccaccgct tcccgcggaa gtcccagttc
     5581 ttcccgtgaa agcgttcgaa tggccgcctt ccaggcattc cgaaatcctt tcagggttgg
     5641 aagctgaatc tcgttcaggt cactaaggac cgaaagaaga cagggtagcg gaagacgctg
     5701 cttctggatc ccttcgtcca ggtttcgtga aacgaccagg tgccgctcgt cagggcatgc
     5761 gaggtcgatg acgtgggtag cgaaaggaat ctcaagaatg gcagccacct cgggcccaac
     5821 ctgcccggtt tctccatccg ttgctttatc ccctgctagg atcaggtcaa aggggcccga
     5881 aaggcgaagc gcggcggcaa gggtaaccgc cgttgtccaa ctgtccgctc cggaaaaggc
     5941 cctgtcggaa atcaacacgg catcatccgc gcccagggac aggcattccc gcaaggcctc
     6001 ctcagcttgt ggaggcccca tggaaaagac cgtcacgctt cctccatgat ttttcgcgag
     6061 gcaaagggcg gcttcgagcg catgaagatc cagcgggttt accgtgcgat ccaccccttc
     6121 tctgatcatg gttcccgttt ccgggtccat tttcacttcg tcggtgtccg gaacttgctt
     6181 ggctaaaacg gcgattttca tggtttggcc tccgtcgctc tggaccgctg ccagaacgtt
     6241 ttcaatccca aacattatct ttttatttaa gtagctgaag gattgacaca aattatactc
     6301 ttggtattat taaatggcaa ggttctttcg agcgaggcga tgggtggaat gacggaacag
     6361 gctcagagtc aaggccgaag gtctcggaag gcggcgggat gggttgcaga ggaaatccgc
     6421 cgcatgatcc tcgaaaatac cctctgccct ggtgaactga tcaatgaatg ccggatcggc
     6481 gagaagctcg gtgtcagtcg aacacccgta agggaagcga tcggccagct tgcccaggag
     6541 gggttgctga aacgaatccc gggcagggcc catcttgttg cggatatgtc cctggacgac
     6601 atcaaggaga tcaacgattt acgaatggtc ctggagcctc ttgcaggtcg ggaagcggtt
     6661 gggcgcattc ctggggaaaa gatccaatcc gccctgcggg aatggaagaa cttccgggat
     6721 ctatttgaaa ggggaaaccc cattctttca agagaaattt cggaggcaga ctccaggctt
     6781 cacggaatga tcatcgacta ttgccggaac agtcgcttaa agacttttct gagcatcctc
     6841 cggtatcaga ttttcaggtg cctcgtggcc tcctgggaag taagggattt cgtcgcggac
     6901 tcgatcgacc agcatatcga gatcctggaa ctgctggaaa agaaagacgc ggacggactc
     6961 gaggcggcgc tccggtctca tatccttttc aacaacaaag tctatcttca gaaagccctg
     7021 taaaaaaaca gccgcaaaaa cgagcgagtg gctctgactt gcacgtacca ttcttttgaa
     7081 cgtggggttt ttccccttcc ataaccccgc aaggactctg gagggatgaa gtgtggaaaa
     7141 agggttcgac tttcgggaac agctcagtgt gatggcacgg gaaaggctgg aaagttcgtc
     7201 catcggtgag atcatcgagc tggctgcacg gcatcgggcc ctttccttcg gggctggcga
     7261 accttcggcg gaactcttcc cgaaaaacgc catccgggaa gcagtggctg aggcttttgc
     7321 ggaaaaggat gtctggggct actaccacga tgacttcggc tacctgccgc tccgggaatg
     7381 gatttgcgag cggatgggaa aagatggaat ggcaccttcc tgggtgcagc cggaacacat
     7441 cctcctcacc aatggcggcg gggaaggggt cagcctcgtc agcgaggcct tgatcgatcc
     7501 gggctccgtc gttcttgtgg aaagcccgac ctacgttgaa agcctgctca ccttccgcaa
     7561 gcagggagcc gtgtgtgtgc ctgtcccttc cgacgacgag gggatcattc cctcggagct
     7621 ggagaggatc gcttcggctg ttcgacctcg ctttctgtac accattccaa atttccagaa
     7681 ccccagcggc agaacgatcc cggaggagcg cagagtccag atccttgaaa tcctgagaaa
     7741 actggatttt ccgatgctgg aggatgaccc ttaccattac ctgagttatg atgctgttcc
     7801 cccaacgtct ttcctgaaac ttacgggaaa tgactacagg gttgtccact gcaacagctt
     7861 ttcgaagaca atcgcgcccg ggctgcgggt cggttggctt gtggtaccgc cgcccctgaa
     7921 gaaggccctc catcacttaa gggtcagcgc cggcctgggc aggccgacgg tgatccaggc
     7981 ggggatctgg cgatatttgt ccggcatcga ttttctttcc cgaatagaag agctttgcaa
     8041 gaattaccgg gaaaggcggg atgccatgat ggatggaatt aaaaggttcc tcaatccttt
     8101 gggaatcagg gccagtcaac cggccggggg gttcttcatc tggtgtgagt ccccggattc
     8161 gagggatatt cattcctttg cgaggtattc agtggaggag cacggaattg cgatcatccc
     8221 cggccaaagc ttcttcccca agggggacaa aaggggggat tgggctttcc ggctttccta
     8281 tgcgaaggtt acggcggagc aggtggagga gggaatgcag cgtctggtac gagcatggga
     8341 atcttacccc gctgcaaggg caacgagcgg tttttagatt gggaaccaac atctaaggag
     8401 ggatcgatgt gaagcggatg tcaaaacggt ttctgattgg ctgcctgttg gcgctgtgca
     8461 tggcggcctt cgcactgcct gcttttgcgg ctgcccccat cgtcatcaag gtggcggcgg
     8521 tgacgagcga taaccatccc gactttatcg ccatgaggga cgtgttcaaa aagaacctgg
     8581 aagcccgctc cggcggacga atcaaggtgg aactctatcc gaacggccag ctgggcggag
     8641 accgggaact catcgagtcg gtgcagatgg gcagcctcga aatcgccatg ccggcttctt
     8701 ccgtcgtcgc gggattcgat ccccgcttcc aggttctcga cctgcccttc cttttcaagg
     8761 ataagaaaac ggcctttgcc gctctggacg gggagctggg aaagaaactg aacgacggga
     8821 tcgtgtccaa ggggttcatc gttcttggat ttactgaaaa cggcttccgt cacgtttcga
     8881 acaaccgcgg gccggtgacc aaaccggctg acttgaaagg attgaagatc cgcacgatgg
     8941 agaaccccgt tcacgtggct ttcttcaagc tgctgggagc aaacccgact cccatcaatt
     9001 atggagagct ctacacggct ctccagcaaa agacggtaga cgcaatggaa tgtccggcgg
     9061 ttctctacga ggacgggaag ttctacgaag tccagaaata cctgtccctc accgggcacg
     9121 tattctcggt ggtcccggtg atgatcggga aaaagttctt cgatgggctg cccaaggacc
     9181 tccagaagat ggtgaaggag gaggcgcgtc gatacgtttt ggcccagcga aagatcatcg
     9241 acgctgagga gaaaaagatt ttcgacgtgt tgaaaaagaa gggcatggtc atcaacgacc
     9301 tgactccggc tcagaagaaa ctttttgtcc aggctaccgc cccagcctac gagcagttcc
     9361 ggggcaagat cggctcggat ctggtggaca tggccatcaa gattgccaac cagtagtaag
     9421 ataggcaata cgaaacgttg ccccgtatcg ggttaacccg atacggggca accctcgtcg
     9481 atagagggat tcgcgaaagg tggaaaacgg tgatgtggga aaggatttcc aagactctga
     9541 acgacgtgga ggagtggttt ctcgtcgtca cgatcgccgc aaatgtgctc ctcgtctttt
     9601 accaggtcat catgcgctac gtcttccaga attccctttc ctggagcgaa gaactggccc
     9661 gctacctttt cctctggtat tcctgggtgg gcaccagcta cgcggtccgc ttcagggggc
     9721 acctgcgggt tgagatgttc gcgaacatgc tcaagggcag gacaaggaaa tggttcgaac
     9781 tccttgtgat cgggatctgg tttctttttt ccctcttcct tgcctaccag ggggtcaagg
     9841 taacccggtt catcgcagaa agccagcaat tgtcttctgc gatgcagatt cccatggcct
     9901 gggcctacgc ctctgttccc gttggctgtg ttctgatgag cctgcgcctt gtgggggatc
     9961 tgtgggatat ttggaaaaaa ctgggctgtg aggaggagcc ggcatagcca tggaagcgac
    10021 cttgcttttc acgacactct ttatcctcat cgggatcagt gttccgatcg gcatcaccct
    10081 gggacttgct tcggccatct gcttctactt cacgaccaac atcccgatga cgtttcttgc
    10141 ccagaagtct ttcacgggtt tggacgcttt tcccttcctg gccattcctt tcttcattct
    10201 tgccggttct ctcatgtgct cgggcgggat ttccaggcga ctggttaacc tggccgaaag
    10261 cctcgtgggt ttcatcgccg ggggattggc catggtcacc gttctggcct gcatgttctt
    10321 tgcagccatt tccggttcgg gacccgccac ggtttcagcc atcggttcct ttatgatccc
    10381 tgccatgaag gaacgcaagt acgacggaag cttcgctgcg gccatcaccg cggctgcggg
    10441 gaccatcgga gtgatcattc ctcccagcat tccgtttgtc atctactgcg tggttgccca
    10501 ggtttccatc ggcgatatgt ttatcgccgg ggtcgttccg ggcatcatca tcggcatttc
    10561 tttgatcacg ctaagctatt tcattgcaaa gaaaaaggga tatgtggctt ccgcagaaac
    10621 cctttcgttc aggaaggcgg ctcacgcctt cagggaagcg atttgggccc tcttcgtgcc
    10681 cgtcatcatt ctgggcggga tttacggcgg catttttact ccgacggaag ccgcggttgt
    10741 tgccgtgatc tattccattc ttattggcaa gtacgtctac aaggagctgg ataaccggca
    10801 gattgtcgag tgcctgaaaa gcacggctct catcaacggc gcactggaat acctgatcgg
    10861 tttttcgatg gcatttgccc aatacctgag cctggcgcac attcctgcag cgatcggtga
    10921 ctggctcacc tcgttttctt ccaaccccta tgtcattctc acgctgatca acattttgct
    10981 cctggtcctg ggctgttttc tcgacaacat cgctgcggtc ctgatcctga cccctattct
    11041 tctcccggta gtcaaaagcc ttggggtcga tccgatccac ttcggcctca tcatttcctt
    11101 caacctggcg atcgggttca tcaccccgcc gtacgggatc aacctcttcg tggcgtccac
    11161 catttccggg gagaacatcg agagcatttc acggtcgatc cttccggtca ttgctaccct
    11221 tgtcggttgt ctgatgctct ttacctattt ccccgttttt tccaccggcc ttgtgcatct
    11281 gctgcgctga gagccattaa gacgcctgca cggctgcaga gaatgggttg gaagagaagg
    11341 ggccccgctt ttttcgcggg gccccttctc ttccttagaa gaccgcgtag tccgaatcct
    11401 tcgggtcgca aaggaacatg tgcccgggcg cgtgggtgat cgcgaagggg ggcttgctgg
    11461 ccatgagggc cgcctgtggg gtcacgccgc aagcccagaa gaccgggact tctccctcgt
    11521 ggatgggtac ggcctggccg aaatcggggt ggttcacgtc ggcgatgccg atgaccgcgg
    11581 ggtcgccgat gtggaccggg gccccgtgga cggccgggaa ccgcccggtg cagagaacgg
    11641 ctttcggaac gagagagtgg ggaatggggc gcatgctcac caccatgggg cccgaaagcc
    11701 ttccggcggg ccggcagggg atgttcgtct ggtacatggg aacgttcagt cccagctcga
    11761 tgtgccggac gggaaccccg gcttcgagca gggccccctc gaaggagaac gagcagccca
    11821 gcaggaaggc cacgaggtcg tcccgccagt attccgtgat ctcctccggt tcggctgcca
    11881 gcttgccgtc cttccacacg cggtacctgg ggaggtccgt gcgaaggtct gcccccgggg
    11941 ccacgatccg cggctcgggg tctccgggtt ccgtcacgtc cagcaccggg cagggcttgg
    12001 gattcctctg ggcgaacacc aggaaatcgt aggcccagtc cttcggcagg atcaccaggt
    12061 tcgcctggac gtgaccccga cagaggcccg aggtgggccg gggccatttc ccggcacgga
    12121 tcgcctcgcg ggcttctttc ggggaaacgt gcgccaggtc ttccatcgtc aggctcatgc
    12181 ggaaagcacc tccctgaggt tggccacggt cacgcccgct tcctccaggg cagcccggac
    12241 cgcggcggcc atcgccacgg cctcgggatt gtccccgtga aggcagatgg aatgaggcct
    12301 gaaatggatc tttttcccgt ccgcggtttc gaccacccct tcggtggcca tctggacgac
    12361 ccgcgcggcg gcgagcctgg ggtcgtggat cacggcccct ggctggctgc gcggggtgag
    12421 ggagccgtcg gactggtagc ttcggtcggc gaaggcttcc gcagcgaagg ggattccggc
    12481 ctttgccgct gcgggttcga agagcgagtt ggccaggccc agcaggacca ggccttcccc
    12541 ggcgtctttt accgcgaagg cgacggcttc cgccagggct tcgcttttcg ccgcctggtt
    12601 gtaaagggcg ccgtgcggct tcacgtgctg cagccgcacg cccgccgcac ggcagacggc
    12661 caaaaggctt ccgatctgca cgaggcagtc ggcatacacc tcgtcgggtg agcaggccat
    12721 gttgcgcctc ccgaacccca ccaggtcggg gtagccgggg tgggccccga cggccacgcc
    12781 ggcatcgcgg gcggacttca cggtcttcac catcaccgag ggatccccgg cgtggaagcc
    12841 gcaggccacg tttgcggaac tcacgtgggc aaggacctcc cggtccatcc ccatcttcca
    12901 ggggccaaaa ctttccccga ggtcgctgtt cagatcgatt tttgccatgg gtttgctctc
    12961 cgtcctttct acagttcctg ccattcgatc aaggcggtct ttccgccgat cctgagggtg
    13021 aaggcgccgg aagttgcttt cacttcgccg ccaacggcct gggggcgcgt gcggtattcc
    13081 gagcgaagct ggcccagggc cgcaaggcgt tttgcccgct cggccagcaa ttttgcccct
    13141 tccccgggag tcgaccgggc aaagcgcacc gtttccccgg gaatccgctg ggccaggacg
    13201 gccaggtccg ccgtcaggac gaccgcgggc ttggtgtagc ccccggttgt ctgccggtcg
    13261 gccagcatga cgatgggctg accgtgtccc ggcacctgga tcgcgcctag cgggatggcg
    13321 tcggacacga tatcggcccc cgtcctgtgg gggatcttcg ggccttccag ccggtagccc
    13381 atgcggtcgg cctggctggt gatcgtgtag gtcgacccga aaagcgtctc aagggcctcg
    13441 ggcgtgaagg catcgtcctg ggggcccaaa accgcccgga tgggagcctc cgggtctcga
    13501 accggccgga gggattcggg gcaggaaaaa ccgatcgtcc ttttccagag gggcaggggc
    13561 tccccgcagg aaaccctgtc gccggccttc agggcgcggc cctcgaagcc gccgatgccg
    13621 gcgcgggtgt aggtggaccg gcttcccatg acgacgggaa cgtcgatccc tcccgagaag
    13681 gccaggtagg cccggcatcc cctgcccttg gggcccgtga aggagatcac gtccccggtt
    13741 ttcacggaga gacaggtcca gaggggcgcc ggggtcccgt tgatcgtcag ccccaggtcg
    13801 gctccagatg cggccaccgt cccctctccc tcgacctcga gggtggggcc cagcagggtg
    13861 atttccaggg ctgcctcgcc gggggagttc ccggccagga ggtttgcgac ggcaagggcc
    13921 tccgggtcca tggccccggc caccggcatc cccatggcct ggtgtcccca acgcccgaag
    13981 tcctggaccg tcgtgctcat cccgcccttt ttgacgttca ggaaaaggcc catgtcaggc
    14041 ggcctccttc cgcaaggcaa ttttcacggt gtactgtccc gccttcgcgg cggcgcggat
    14101 ggcctcgtat tccgactcgc tgatggcctc gaaccggacc cagtcccccg cttcgaccag
    14161 tgtcgggggg gtcctcaggg gatcgaacac cgccagcggg gtccgcccga tgagctgcca
    14221 gccgcccggg ctgtcgatcg ggtagatgcc ggtctgcttt cccgcgatgc cgacgctgcc
    14281 ggcggggatg agcgtgcggg ggttctccag ccgcggcgtt gccagggatt cgtccatccc
    14341 gcccaggtag gcgaaccccg gggtgaaccc cagcatgaag caataggtgt ccctcccggt
    14401 gtggcgggcg atcacttctt ctttcgaaag gcccgtgtag gcgcagacat tttccaggtc
    14461 ggggccgaaa gttcccccgt aacagaccgg gatcgtgacc acacggcttt ccgcttgtga
    14521 agcttcatcg agggtttcgc aggcgctttc gatccgtttc ttcagttctt cccggtccaa
    14581 aaggaggggg tcgtaataga ccgccagcga gcggtaggtc gggaccagct cgacgattcc
    14641 cttgacggcg cactttgcca gggcgtgttt cagcgcctgg actttcgcgt tgagcgcccg
    14701 gtccacggac gcaccgaact ccgcgaccag gcagctgtcc cccgcatcga gcaggcgcgg
    14761 ggttctgggc tcttccattg gattcacctc gattcaaaaa agggggacgg ggaagccccg
    14821 tcccccgcca aggccattct acttgatcag ttcgacgatt ttgctaaggg actgccaccc
    14881 catccaggcc gtgaacgcca ccatgacgaa acccagggcc gtcaggatct tcgggtgcct
    14941 gtagtctccg acgatgtcct tcctgtgggc tgccaggagc atcgagagaa gggaaagggg
    15001 caggatcagg ccgttgatcg agccggcaaa gatcagcagg ttcaccggct tcccgacggt
    15061 cgtgaagatg gtggtggagg caacgatgaa cccgatgatc caccagcgct ggtactgctt
    15121 cacgtttttg aacagggtct gcaggaagga gatggaggtg taggaggccc cgacgacgga
    15181 cgtcacggcc gcggcccaga ggacgacgcc gaagatcttg tagccgatca ttcccgcgcc
    15241 gatctggaag gcggaagccg gcgggttcga gggatcgagg ggcttgccca tggccacgac
    15301 gccgaggatg gccaaaaaga gcaaaaagcg catgattccc gtgatggcaa tgccctggac
    15361 ggagccgtag ctgatctgct tcaccttgtc ctgccccgtg atgccagcgt cgatgatccg
    15421 gtgggcaccg gaaaaggtga tgtagccgcc cacggttccc ccgaccagcg tcagcaccat
    15481 catccagtcg atcgtgctgg gcaggacgga ttcctttagg gcgaggccaa cggggggctg
    15541 ggtcttgaac accatgaaga gcacgagggc gatcatcagg aagcccagga ccttcgtgaa
    15601 ggtgtccatg gtcttcccca tctccttgtt caggaagatg aagatggcca gggcggcgct
    15661 gacgatggcc cccaccgcca tgttggaccc gaagaggacg ttcacgccca gcgccgcgcc
    15721 ggcgatgttg ccgatgttga agaccaggcc gccgagggcc accgcgaagg ccaggaagta
    15781 cccgaggccc gggatgacct tgttggccac gtcttgggcc cgcatgccgg aaacgcccag
    15841 gatccgccag atgttcagct ggacgatgac gtcgatgagc acgcttgcga ggatcgcgaa
    15901 ggcaaaggct gccttcagct tgtcggtgaa gaccgccgtc tgggtcaaaa acccggggcc
    15961 gatggccgat gtggccatga ggaatgcggc ccccagaagg acgctcagga cctccttgcg
    16021 ggatgccacg gcctcgccga gcgagccggg ctggggcagg gtgtgattcg ttggttcgga
    16081 catgggagca cctccggaaa aaatgaagtt aggtttctat aaaggatata agcaaaaaag
    16141 ttgccgaatc gaaccggctc ccctgtctgg cattcaactg cctggaaatc caggcggcga
    16201 gagagaaagc gaaggtcact tcccggagga aaaaaacttc ggagggagac acggcgggct
    16261 tgtgtgcgac acgctgtgcg gtcaggcgcg gcggacaggc ggcaaaccca gccgctgacg
    16321 atgccaggtc actgtgcggg gggggagccc cagctcccgg gccaggacgg aatcgggctt
    16381 ccccgattcc cacccggagc ggatggcctc cctgagggca cgcgtggaaa gatcggggcg
    16441 ggccgcaatc cctcttccca ggagggaaga aagcggcagg gtgcctcggg gggtcaccgc
    16501 ccaggtgcaa gcggcggctc gctgaacggt cgaagcggaa agccccagct tttggcccgc
    16561 aggggccagg gtcaaaggaa caggggccga ggcaaggttt ctcaggtagg cctgctggcg
    16621 ctccgccagc aggagggcaa gccgcatctt cgtgcggtac cgcgcggcca gcgcggaaag
    16681 gagtccccgc gcctcccgga aaggcccgcg ggcgacgtcc ccggccgcgg cgtacagctc
    16741 cgcatccagc aagacccggg gcaggttctc gtggaggagc ctgaccgtgg gccttccccg
    16801 ttcgtcgtag cggatttcga cttccggcaa gaccgtcccg ggatgggaaa aattgaaccc
    16861 cgggtgggga tccagccgcc tgaggcttcg gagggccgcg tccaggcgct gccgttccca
    16921 ggccagtttc ttccgcagcg cagccaggtc ctgccgggct agttcctcca gcccctcttc
    16981 cagcaggatc caggcgtcgg agccctccag cccctgcctg gaaagctgga gcctgagaca
    17041 gtggggcagg tc
//
LOCUS       DAOOVN010000023        14780 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_2714_length_14780_cov_15.251613, whole genome shotgun
            sequence.
ACCESSION   DAOOVN010000023 DAOOVN010000000
VERSION     DAOOVN010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 14780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14780
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2714_length_14780_cov_15.251613"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..503
                     /locus_tag="PLY83_03605"
     CDS             39..503
                     /locus_tag="PLY83_03605"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOV29156.1"
                     /translation="MKLLQAATPALIAQARCLFREYKECLNNDAWFPRFEEELDNLPV
                     PYAPPSGRLLIGCVEGKPAGCIAFKAGDSPDFCEMKRLYVRPEYRGMKLGRSLAELLI
                     VEAVNAGYKSMRLSMIPAMREAMGLYTSLGFREITPYRNSPCDGTVFLELPL"
     gene            complement(531..1796)
                     /locus_tag="PLY83_03610"
     CDS             complement(531..1796)
                     /locus_tag="PLY83_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29157.1"
                     /translation="MIVMIPSRSIQTNRKSGKRSILSFVLAGLMLGFLGIALFGGRAC
                     FADPPEEEGWFFPYIGSGNGLWEIDAGAFFWSDRFEVRNLQLLTDIDIAPGVRWHSLV
                     RSNRERDTLAGFEPFFDEGYLEMYGFHRGKKGQFSASLKVGVFRYLHFPYPDAIAVFD
                     QVPGISDLNGGEKTGYGGSCLVLDYAHDSGWGLHFTGLEWGFGYPDQDSVIEAYLSFK
                     KEWGQGYRFEARVGKLQVRPEPLGRGERGWNVFFGKAFEAGSVGFLYEKLENQPAYTG
                     LMVTFPVSPKTQLLGSVAFDYARSPEGLAVHLPVAWGTIGRVSRRVPDNSEAVGGLKA
                     ERLRTYWQNSQVRNYYEHRLDHWGETYEKGLFVVMEEEPWTLQAEALVSPHTSFGSWD
                     DLRDWERDRQGPAQISQEVTYRFYRVEPR"
     gene            1927..4866
                     /locus_tag="PLY83_03615"
     CDS             1927..4866
                     /locus_tag="PLY83_03615"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOV29158.1"
                     /translation="MPVSIQEYRKVPEGLAAAAEMDGPGTVFVVPSREDAKILREALC
                     GGGCFEPSGRVWAMAELYRKTLAGLEEAGADPAWHRQVDPPDHWAILSFLLDRFFSEA
                     VPSTQPPPGARMPGFVPLLGEQLRELLREETPPDALGRSLGCNTCLEGSCANLSLPEG
                     LLCRLFRAYNRYLSEHGLADSASLPTLTRQALERHPREAAAFLAGKRFVFAGFLSFTR
                     GQASLILNLAGLGGRRIVLKTTTGLKSFADAVDQLLSLPDRSGPAVRLLPSRGDPLPV
                     FELSAGTTPMEADTAARTLALWRWGEGPLAAFPFPGWDGITVQSDSFQAGRFAEAFAR
                     YRIPFFQADGVPVSSTAAWTFTGAVLDAAKAGWPPAETAAVLADPLAAGSRFPLSAAR
                     SAYPQGESSWRTFLDALQDRLPAEAFSRAVAFSRKVEEGAPPAELLKALKTLWGRRNG
                     SGTALSRLAEDQPDLDGFVRQAEEAFRELERKILLEEERFPSIGPARERRLRGKEARA
                     YLSEWADETAIRPPAPLAGAVALFSGPPPVWFSRRCFVLAGGTAKAWPGPLKEAPLLE
                     DAVRSVLNDRSAEGGGGRLPLLADRRLQQEALFRRILSAGESLAVLSRAEADDQGRPL
                     EKTPFLEAAVSSNWFLPGGAALRPLSAALPRPGEPFFPQVEPVPKAPRVVRTPLPPAA
                     FREGGPALRASLGDLDLWLDCPFRYWAQKLAGLEEPSRGLFDPARAGTFLHALWQEVW
                     RRRLEEGGGSLACLVEELWNGTLADPDPRRGYPALAQDPRLLRRAGRLKELALRLARF
                     QEELETVFAPARKAQDREIPMSLEVEGVTFRGRADRVEVFDRGFVVLDYKSGKSSAYK
                     TSLQLAAYSAVLKDVLGLAPWGTGFFGHADGRLFLALSEDCPLSFPRRNGTVLAKAGT
                     LEESIRQAQEGILAMARGLRAGSYPAAHGTASGCPHCFARGLCRLGEARGEILTEETE
                     TEETLDD"
     gene            4859..>7824
                     /locus_tag="PLY83_03620"
     CDS             4859..>7824
                     /locus_tag="PLY83_03620"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOV29159.1"
                     /translation="MIENAASALLETLLQEARPEQREAVEAGEPLVVVGAGAGTGKTK
                     TLAWRFLWALLAFPQTRVENILTLTFTEKAAREMKERIARMIAEALEAARALGLEETA
                     LRLSDAGARLDEAYISTIHAFALRVIRENGLALPVDPKAGIVSAPREEAFWETFTAAL
                     DSLVPEDGSRGLAEPWKSRAESLFSSPVLRSAVNTFGPEAVAEFCRSAAALHGSRGRL
                     PEDLWNWSPGEDEPVRLALLGERAPSWKERLERFRAEVFPGLGDLSKERTALGKKLDA
                     LSRTWREGSVGEDPCRLLAFLEELDAALKGASGKLKDTLSDLLGQTPSELRKELEKEL
                     PLARSLVKGLSEEEQAFRQVLAQTAAVAWARWEQAKSESGSLSFDDLIRHASSAVLAN
                     PAYGKRFHHLLVDEVQDTDPLQDDLIEALWEAGGGRLFLVGDLKQSIYRFRHADPALF
                     ARRIREEAKGTAGRYILLDRSYRMREELVRFVNGCFGILWDDELGKGLGVAYEALRGP
                     DDAPWWRERNELETEALSVLLEPHRVDPDTGKPEKKAKVRRRLADRLAATLAALRGKP
                     AWDKEGAFRRPLSWRDMAVLVPGRTQYPALQEAFEAAGIPAVFQDSQGFFSRGEVFDL
                     VSLLKALADPGDRLALAGWLASPFSGLRKEDALKLADSPGGDDPPLSERLRARFPDAW
                     KRFEALRSQALLAGPGAALAALLNEPATLLTAPEADRPRMAANLARAASLAREFAAAR
                     GQNLAACAESLGSALRRALPAEEPDFFSEDEDVVRVMTVHAAKGLEFPVVAILGLDDG
                     GRGNHRGTSLVPSVFLTGVSTSIPGEESQEAQAVLWHRALEEQALEEEWERLFYVACT
                     RAQDVLLLCGICPVEAETGIPRPKEGTWLSMLEKASPGVLQAGLQDPMEATPETRPIP
                     GIFSLSPGAEGKAVPLPSDLPTLASVSATGYALFSFCPRAWRLQVRQGLELAAEKPGP
                     GEPGGPDLGSLAHRVLA"
     assembly_gap    7825..7924
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7925..8257
                     /locus_tag="PLY83_03625"
     CDS             <7925..8257
                     /locus_tag="PLY83_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29160.1"
                     /translation="WEEDGIVHLADYKFAAPSTFLELLGKNQLAFYGAVASRLFPGRT
                     IDLSLVFLKESEGRLPFEGFLDAEAVETRVRAMARAGWTGPFARNPRSCPACPWREPC
                     GESAGSLE"
     gene            8298..9137
                     /locus_tag="PLY83_03630"
     CDS             8298..9137
                     /locus_tag="PLY83_03630"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOV29161.1"
                     /translation="MKPSGSGLVQRALAFACFCLYFAYVLVPILLLLAGSFGERWFST
                     LLPEGFTLKWYVDLFTNPMYTKAIGRSLLVAVLTALINAGVAIPAVYAVHTLNLRWLR
                     NLYDSVILLPIAVPPIVMGLGLIQSFNWPGFSLVGTWQLLLGAHVVYTLPFTLRPLLA
                     NLEMIQWNVLEEAGLSLGAGMPFLFRRVLVPNLMPGILSGALMTFAMSLGEFQLAVML
                     TSSNSQTYPVVLYQAFYVSTGFACSATMVLIVTSLFTLMGVLFLGRLFGMGKAQILFG
                     GGS"
     gene            9139..9612
                     /locus_tag="PLY83_03635"
     CDS             9139..9612
                     /locus_tag="PLY83_03635"
                     /inference="COORDINATES: protein motif:HMM:NF037785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HOV29162.1"
                     /translation="MASRLVGVIGTSNASPSIAALAFETGRLLAQKGCTVLCGGMGGV
                     MEEAARGAASAGGTSIGLLPREASEANPYLTHAIATGMGEARNFLITHASPVLIAIGG
                     GLGTLSEIAMALRLGKRVVALRTWTARDEENNPLPVLPAQSPEEAVRLALGEPGP"
     gene            9609..10661
                     /locus_tag="PLY83_03640"
     CDS             9609..10661
                     /locus_tag="PLY83_03640"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV29163.1"
                     /translation="MSGVELRNVTKAFGKAVAVRNLDLAVGKGEFLALLGPSGCGKTT
                     TLRMIAGFTRPDSGEILIDGQPVSSLSVRERRVGIVFQSYALFPNMTAFENIAFGLRV
                     RRMPEEEVRRRVFRLLEMVGLEDKANASCRQLSGGQQQRVALARALAIEPRVLLLDEP
                     LSALDAKVRNSLRFEIKKIQRESGITTIYVTHDQEEALSISDRVAVMRYGQIEQAGTP
                     WEVYSNPSSLFVADFVGVSNFLDVEVKAGGAVFWNDRPLDVEGAGSLAPGPWRLVIRP
                     ERLLPVPKDAQGQNILEGTVAGRVFLGPIQRVALDVGKERILVDLLNTEKGHLSPGEP
                     LRVSFSPGDGRLIHRS"
     gene            10717..11787
                     /locus_tag="PLY83_03645"
     CDS             10717..11787
                     /locus_tag="PLY83_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOV29164.1"
                     /translation="MKRKFIALMSILLLAAFAVQAAAAELYPGENALLQKAKKEKGLN
                     SYDTGPTWANWQGLFDAFKARYGINITYNDLGSGATVVRLEKEKNNPQADTAYYFMPF
                     GALAKEKGVTQPFKPVNFEKIPPVLKDKEGHWFCIHKGTVVFVVNKRLVKESPKSWKD
                     LLDKKYAKSVVYLDPRTTGIGYAIVIAAAYANGGSIIDLSKGVDYLAALQKAGNVRMI
                     EKTTEYDKFLKGEIPIWITYDWNAYRAKYIANLGDAVEIILPKDGTITAPYAISMAKG
                     APNPNTAKLWLNFILSEEGQGIFAKGFVRPILPGVKLPPEVAGKFLPASEYERAYDVD
                     WVLAQKVQEDAAKMWGRRVLGE"
     gene            11809..12639
                     /locus_tag="PLY83_03650"
     CDS             11809..12639
                     /locus_tag="PLY83_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOV29165.1"
                     /translation="MERRGWKFLLLLPLAAVLGIFLVLPLLFVLYDSFWAAGRLSAAN
                     YLLVFRKGLYSEALRTSLVLSVSTAFIGAALGLPLSYLVHRSRAPWRDLLLAITAVPL
                     TFSGLVIGFAFIVLLGTSGFITMLLERFFGINPLEFSAFLFTWKGLVVAYLYFLIPRM
                     ILTMVAAWSNADWSQVEAAMSLGASFPTILVKILFPMLGPSLLAGSSLLFAVSMGAFG
                     TAFALTGTAVKILPLVIYNQISDVTVEVSRANALAIVLTVVTTLVIVLYERSFARKQA
                     "
     gene            12785..14176
                     /locus_tag="PLY83_03655"
     CDS             12785..14176
                     /locus_tag="PLY83_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOV29166.1"
                     /translation="MPVLLLQILVFGAYTLALVFLAGEAFNWKEDRRLFYVGGNRLTL
                     VPMVGTFVATWMSAASVLGYTMWLYADGYVAFTGSVNGWLIGLLFMPFIVRKLRASRA
                     LSLPQWLGDTFGDRRLRSLSAVALIGAYTFYMVIQFRAFGTIVATLLDIKVIVASALV
                     YLFVLYTTFGGLPSVARSDTLNLALILGGVLLAAGTLLKRAGSLAAVHAAIAERAPAM
                     LSPWSEGWSPLVTTLMMLSWGLGVAANPQYAVRLLSARTTRDAYGTLATSSLIIGAIY
                     AGLTVLGLSAFVLFPGVRAASDELAFTRLFSLALPPLPALALFLAVLAAAVSTANSQL
                     LLAACSLCYDLSPREGRNRGDLVTEDRFLFKNRLAIAGIATAALVLSQFRLPGVLLIG
                     RYSWTVVAICFFLPLYVIRTPRPRALFVPLSAALLLHLFLTVFLGLLPELALLPALAL
                     EGLLYFGPWRKKS"
     gene            14173..>14780
                     /locus_tag="PLY83_03660"
     CDS             14173..>14780
                     /locus_tag="PLY83_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HOV29167.1"
                     /translation="MRSPRPLSIETRIKVILTAIAMGLTGLTVGTALTQGFFERTGEA
                     RLLLETARSAASGLPAAQVEPLLARLRPFWKEAPRAQGAETVRSADGQEWRFVLDRAA
                     IWNALVYRQRYVLVAGLIGLLVAVELAVFLAYSLTRPMKILAWGCEQMRSGRWVRLPT
                     LSRTPYELEALNEAFNGMVEDLKRWQDFQKQAARVERLAALGS"
BASE COUNT         2434 a         4780 c         4788 g         2678 t
ORIGIN      
        1 gttgattctc gatgaaatgg ggcgggaaga tgagcaccat gaagcttctg caggcagcga
       61 cgcccgcgtt gatcgcccag gccaggtgcc ttttcaggga atacaaggaa tgcctgaaca
      121 acgacgcctg gttcccccgc tttgaggaag agctagacaa cctcccggtt ccctacgccc
      181 cgccgtcggg gcggctgctg atcggctgcg tcgaggggaa acccgcgggc tgcatcgcct
      241 tcaaggcggg ggacagcccg gatttctgcg agatgaagcg gctttacgtc cgcccggagt
      301 accgagggat gaagctgggc cgctccctgg cggagcttct gatcgtggag gccgtcaacg
      361 ccgggtacaa gtcgatgcga ctgagcatga tcccggcgat gagggaggcg atgggactgt
      421 acacctcgct ggggttccgg gagatcactc cctaccgcaa cagcccctgc gacgggaccg
      481 tctttttgga actgccgcta taaatcggcg accgacagca gaaggaaggc tcagcgcggt
      541 tcaacccgat agaaacggta ggtaacctcc tggctgattt gagcgggacc ctgccggtct
      601 ctttcccagt cccgaaggtc atcccagctg ccaaaggaag tatgggggct gaccagggcc
      661 tcggcctgaa gcgtccaagg ctcttcttcc atgaccacaa agagtccttt ttcataggtt
      721 tctccccaat ggtccagccg gtgttcatag tagtttcgaa cctggctgtt ctgccagtag
      781 gttcgaagcc gttcggcctt tagccctcct actgcctctg aattgtccgg cactctccgg
      841 gataccctgc cgatcgtgcc ccatgctact ggaaggtgaa cggccaatcc ttccggtgat
      901 cgggcataat caaaggctac agaaccgaga agctgcgttt tcggcgaaac aggaaacgtg
      961 accatcaacc ccgtgtaggc cggctgattt tcgagcttct cgtaaagaaa ccctaccgat
     1021 ccagcctcga acgccttccc gaagaaaacg ttccacccac gctcccccct ccccaggggc
     1081 tcggggcgga cctgaagctt ccccactctg gcttcgaaac ggtagccctg cccccactcc
     1141 ttcttaaagg agagataggc ctcaatgacg gaatcctgat cgggataccc gaacccccat
     1201 tccagccccg tgaaatggag cccccatccg gaatcgtggg cgtaatccag caccaggcaa
     1261 gatcccccgt accctgtctt ttctccccca ttcaggtcag aaatcccagg gacctggtca
     1321 aaaaccgcga tcgcgtccgg ataggggaaa tgcaggtacc ggaagacgcc gaccttcagg
     1381 ctggcactga actggccctt tttcccccgg tggaaaccgt acatttccag gtacccttcg
     1441 tcgaaaaaag gttcaaagcc ggcaagggtg tccctttcgc ggttcgagcg taccagggaa
     1501 tgccagcgaa cacccggagc gatatcgatg tcggtcaaaa gttgaaggtt tcgtacttcg
     1561 aaacgatccg accagaagaa agcgccggca tcgatttccc aaagtccgtt cccggagccg
     1621 atgtagggga aaaaccaccc ttcctcttca ggagggtcgg caaaacaagc cctccctccg
     1681 aaaagggcga tcccgaggaa gcctagcatc aaccccgcaa ggacaaagga caggatggag
     1741 cgtttgcccg atttccggtt tgtctgaatc gaccgtgaag ggatcatgac gatcatctta
     1801 ggaagctttc ccgaaatacg caagcttctc ctcctggtgt tcgccccttc tgtttggaaa
     1861 ttggaataag atggaagaac tatcctgccg aaaacagcct ccggcgaatc tctgggaggg
     1921 aagcaaatgc cggtgagtat tcaggaatac cgaaaggtcc cggaagggct tgcggcggcg
     1981 gcggaaatgg acggcccggg caccgtgttc gtggtcccct cccgggagga cgcgaagatc
     2041 cttcgggagg ccctgtgcgg aggcgggtgt ttcgaaccct ccgggcgggt gtgggcaatg
     2101 gccgagctgt accggaagac cctggcaggg ctggaggagg cgggtgccga ccccgcgtgg
     2161 caccgccagg tggatccgcc ggaccactgg gcaatcctgt ccttccttct cgatcggttc
     2221 ttttccgaag ccgttccctc tacccagcct cctccgggag cgagaatgcc gggctttgtc
     2281 ccccttttgg gggagcagct acgggaactg ctccgggagg aaacgccccc ggacgccttg
     2341 ggacgatccc tgggatgcaa cacttgcctc gaaggttcct gcgcgaacct ttccctcccc
     2401 gaggggctgc tgtgccgcct tttccgcgcg tataaccggt atctttccga acacggcctg
     2461 gccgatagcg cttccctgcc gacgctgacc cggcaggcgc tcgaacgcca tccccgggag
     2521 gcggcggcgt ttttggccgg gaagcgtttc gtgttcgccg gtttcttgag cttcacccgc
     2581 ggacaggctt ccctgatcct gaacctggcg ggtctcggtg gtcgccgtat cgttctaaaa
     2641 accaccaccg ggctcaagag cttcgccgac gcggtcgacc agctgcttag cctgccggac
     2701 cggtccggcc cagccgtcag gcttttgcct tcccggggcg atcctctccc cgttttcgag
     2761 ctctccgcgg ggacgacgcc gatggaagcc gataccgcgg ccaggaccct tgccctgtgg
     2821 aggtggggag aggggcccct ggcggctttc cctttcccgg gctgggacgg catcacggtc
     2881 cagtccgact ccttccaggc cggccgtttc gcggaggctt tcgcccgcta ccggattccc
     2941 ttcttccagg cggacggcgt tccggtttcc tccaccgcgg cctggacctt caccggggcc
     3001 gtgctggatg ccgcgaaggc cggctggcct cccgcggaaa ccgcggcggt cctggccgat
     3061 cccttggcag ccggaagccg gttcccgctt tccgctgccc ggtcggccta cccgcagggg
     3121 gagtcctcct ggaggacgtt tttggacgcc ctccaggacc gtctgccagc cgaggcgttt
     3181 tcccgcgcgg tggcgttctc gcggaaagtc gaagaggggg cgcctccggc ggaacttcta
     3241 aaagcgctca agaccctctg gggacgccgg aacggttccg ggacggctct ttcccggctg
     3301 gcggaagacc agccggacct ggacggcttc gtccggcagg ccgaagaagc cttccgggag
     3361 ctggagcgca agattttgct ggaagaagag cgtttcccgt ccatcggccc cgcccgggaa
     3421 cggaggctgc ggggaaaaga agcccgggcc tacctttccg agtgggcgga cgagacggcg
     3481 attcgccctc ccgcgccgct ggccggcgcc gtggccctct tttccggtcc gcccccggtc
     3541 tggttttccc gccgctgctt cgtcctggcg ggcgggacgg caaaggcctg gcccggcccc
     3601 ctgaaggaag ccccgctgct tgaggacgcg gtccggagcg tcctgaacga ccggtccgcg
     3661 gagggaggcg gcggccgcct tccgctcttg gccgaccgcc ggcttcaaca ggaggcgctt
     3721 ttccggcgga tcctctcggc cggggaatcc ctggccgtcc tgtcccgggc agaagcagac
     3781 gaccaggggc gcccgctgga aaagacgccc tttctcgagg cggcggtttc ttcgaactgg
     3841 ttcctgcccg ggggagcggc gctgcggccg ttgagcgctg cccttccccg cccgggcgag
     3901 ccgttctttc cgcaggttga gcccgtgccg aaggctcccc gggtcgtccg aaccccgctt
     3961 ccccccgccg ctttccgcga aggggggccg gccctccggg caagcctcgg agacctggac
     4021 ctgtggctgg attgcccctt ccgctactgg gcgcaaaagc tcgccggcct ggaagaacct
     4081 tcccgcgggc tgttcgaccc ggccagggcg gggacgttcc tccatgcgct ctggcaggag
     4141 gtctggaggc gtcggctcga ggaaggcggg ggaagccttg cctgcctggt ggaagagctg
     4201 tggaacggca ccctcgccga tcccgacccc cggcggggct acccggccct ggcgcaggac
     4261 ccacggctgt tgcgaagggc gggacgcctg aaggagctcg cccttcggct ggcccggttc
     4321 caggaagaac tggaaacggt tttcgccccc gcgcggaaag cccaggaccg ggaaataccg
     4381 atgtccctgg aagtcgaggg agtgaccttc cgcggccggg ccgaccgggt ggaagtgttc
     4441 gaccggggct tcgtcgtcct ggattataaa tcggggaaaa gcagcgccta caaaacgtct
     4501 ctccagcttg cggcctattc ggccgttctc aaggacgtcc tgggcctcgc cccctggggg
     4561 accggctttt tcggtcacgc agacgggcgg ctgtttctcg ccctttcgga agattgcccc
     4621 ctttcgttcc caagaagaaa cggcaccgtc ctggcgaaag cagggactct cgaggaatcg
     4681 attcggcagg cgcaggaggg gatcctggcc atggcgagag gcctccgggc gggatcctac
     4741 ccggcagccc acgggacggc gtcgggctgt ccccactgct ttgcccgggg gctttgccgc
     4801 ctcggggagg cccgcggaga gatccttacc gaagaaacgg aaacggagga aaccctcgat
     4861 gattgagaac gccgcgtccg ctttgctcga aacgcttctg caggaggccc ggccggagca
     4921 gcgcgaggcc gtggaggcag gcgagcccct cgtggtcgtc ggtgccggcg cgggtacggg
     4981 caagacgaaa accctggcct ggcggtttct ctgggccctc ctggcgttcc cgcagacgcg
     5041 ggtcgagaac atcctgacgc tgaccttcac cgaaaaagcc gcccgggaaa tgaaggagcg
     5101 catcgcgcgc atgatcgccg aggcgctgga ggcggcccgg gcgctgggcc tggaggaaac
     5161 cgctctccgc ctgtccgacg cgggtgcccg gcttgacgaa gcctacattt ccacgatcca
     5221 cgccttcgcc ctgcgggtga tccgtgaaaa cgggctcgcc ctgccggtgg accccaaggc
     5281 cgggatcgtg agcgctcccc gcgaggaagc cttctgggaa accttcaccg ccgcgctgga
     5341 cagcctcgtc ccggaggacg gctcaagggg gcttgccgaa ccctggaaaa gccgggcgga
     5401 aagcctgttt tcctcccccg ttctcaggtc ggcggtcaac accttcgggc cggaagcggt
     5461 ggcggaattc tgccgctcgg cggcggcgct tcacggaagc cggggccgct tgccggagga
     5521 cctgtggaac tggagccccg gcgaggatga acctgtgcga ctggcccttc ttggggaacg
     5581 agcgccctcc tggaaagaac ggctcgagcg gttccgggcg gaggtcttcc cgggcctggg
     5641 agacctgtcg aaagagcgga ccgccctggg gaaaaagctc gacgccctgt cgagaacctg
     5701 gagggaaggc tccgtcggcg aggacccctg ccggctgctg gccttcctgg aggaactgga
     5761 cgctgcgctc aagggtgcaa gcgggaaact gaaggacacc ctttcggacc tcctggggca
     5821 gactccctcg gaactgagga aggaactgga gaaggaactt cccctggcgc gttccctggt
     5881 caagggtctt tcggaggagg aacaggcttt ccgccaggtg ctggcgcaga cggcggccgt
     5941 ggcctgggcc cgctgggagc aggcgaagtc ggaaagcggg agcctctcct tcgacgattt
     6001 gatccgccac gcctcttcgg ccgtcttagc caacccggcc tacgggaaac gctttcacca
     6061 tcttctcgtg gacgaggtcc aggacaccga ccccctccag gacgacctca tcgaagcatt
     6121 atgggaggcg gggggcggcc gcctgttttt ggtgggcgac ctgaagcagt cgatctaccg
     6181 gttccgccac gccgacccgg cgcttttcgc ccgcaggatc cgggaagagg cgaaaggaac
     6241 cgcagggcgg tacatcctgc tggaccggag ctaccgcatg cgggaggaac tggtccgttt
     6301 cgtgaacggc tgtttcggca ttctctggga cgacgaactg ggaaagggac tcggcgtcgc
     6361 ctacgaagcc ctgcggggac ccgacgacgc cccctggtgg cgcgagcgga acgagctcga
     6421 aacggaagcg ctttccgtgc tgctcgagcc tcaccgggtg gatcccgata ccgggaagcc
     6481 tgaaaaaaaa gccaaggtcc gccggaggct tgccgaccgg ctggcggcaa cgcttgcagc
     6541 cctccgggga aaacccgcct gggacaagga aggggctttc cgccgccccc tttcctggag
     6601 ggacatggcg gtcctcgttc ccggaaggac gcagtacccg gccctgcagg aggctttcga
     6661 ggccgccggg atcccggcgg ttttccagga cagccagggg ttcttctccc ggggggaagt
     6721 cttcgacctc gtctctcttc tgaaagccct ggccgacccg ggtgaccggc tggccctcgc
     6781 cgggtggctg gcctctccct tctcgggcct tcggaaggaa gatgccctaa agctggccga
     6841 ttcccccggc ggggacgacc cgcccctttc cgaacggctg cgggcaaggt ttcccgatgc
     6901 atggaaacgg tttgaagccc tgagatcgca ggccctcctg gcggggcccg gagctgccct
     6961 ggcggccctc ctgaacgaac cggcgacgct tttgaccgcc ccggaggccg accggccccg
     7021 gatggcggca aacctggcgc gggccgcgtc cttggccagg gagttcgccg ccgcccgggg
     7081 gcagaacctc gcggcctgcg ccgaatccct gggaagcgcc ctccggcggg ccctcccggc
     7141 agaagagccg gacttctttt ccgaggacga ggacgtcgtt cgcgtcatga cggtccacgc
     7201 cgcaaaaggg ctggagttcc cggtggtcgc gatcctgggc ctggacgacg ggggccgggg
     7261 gaatcaccgc gggacctccc tggttccttc ggtgtttctc accggcgtgt ccacctccat
     7321 tccgggggag gagagccagg aagcccaggc ggttctctgg caccgggccc tggaagaaca
     7381 ggcgctggaa gaagagtggg aacgcctgtt ctacgtggcc tgcacgaggg cccaggacgt
     7441 cctcctgctg tgcggcatct gcccggtgga agccgaaacc ggaatccccc ggccgaagga
     7501 agggacctgg ctctcgatgc tggagaaagc atcgccggga gtcctgcagg ccgggctgca
     7561 ggaccccatg gaggctaccc ctgaaacccg gccgatcccc ggaatttttt ccctttcccc
     7621 cggggcggaa ggcaaagccg tgccccttcc gtcggacctg ccgacgcttg catcggtttc
     7681 ggccaccggc tacgcgctgt tttccttctg cccccgggcc tggaggctgc aggtccgcca
     7741 ggggctcgag ctcgccgcgg aaaaaccggg accgggcgaa ccgggaggtc cggacctggg
     7801 gagcctggcc caccgggtcc tggcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nnnntgggag gaagacggga tcgtccacct ggcggactac aagttcgccg cgccgtcgac
     7981 tttcctggag cttttgggaa aaaaccagct ggccttttac ggagccgttg cgagccggct
     8041 ctttcccggc aggacaatcg acctgtccct ggttttcctg aaggaaagcg aaggacgcct
     8101 tcccttcgaa ggctttctcg acgcggaggc ggtggagacc cgggtccggg cgatggcccg
     8161 ggccggctgg acgggcccgt tcgcccgcaa tcctcgatcc tgtcccgctt gtccgtggcg
     8221 cgagccttgc ggggaaagcg ccgggtccct agaataaagg gagattgtgg cagactgaag
     8281 cggtgaggag gttcgggatg aaaccgtccg gcagcggcct cgtgcagcgc gccctggctt
     8341 ttgcgtgttt ctgcctgtac ttcgcctacg ttctcgttcc gatcctgctc cttctggcgg
     8401 gatccttcgg cgagcggtgg ttcagcaccc tcctgccgga agggttcacg ctcaagtggt
     8461 acgtggacct ttttaccaac cccatgtaca ccaaggccat cgggagaagc ctcctggtcg
     8521 cggtcctgac agccctcatc aacgcgggcg tggcgatccc tgccgtgtac gcggtccata
     8581 ccctcaatct ccgctggctg cggaacctct acgattccgt gatcctgctg ccgatcgcgg
     8641 ttccccccat cgtcatgggg ctgggcctga tccagtcgtt caactggccc ggcttttccc
     8701 tcgtgggaac gtggcagctt ctgctgggag cccacgtggt ctatacgctt cccttcacgc
     8761 tccggcctct tttggccaac ctggagatga tccagtggaa cgtcctggag gaagccggcc
     8821 tatccctcgg ggcgggaatg cccttccttt tccgtcgggt gttggtgccg aacctgatgc
     8881 cgggcatcct ttccggggcg ctgatgacct tcgccatgtc gctgggcgag ttccagctcg
     8941 cggtgatgct caccagctcg aactcccaga cctatcccgt ggtgctttac caggccttct
     9001 acgtgagcac cgggtttgcc tgctcggcca cgatggtgct gatcgtgacg agcctgttca
     9061 cgctgatggg cgtcctcttc ctcggaaggc ttttcgggat ggggaaagcc cagatcctgt
     9121 tcggaggagg ttcctgagat ggcaagccgt cttgtgggcg tgatcggcac atcgaacgcg
     9181 tcgccgtcca tcgcagcgct ggcctttgaa acgggccgcc tgctggcgca gaagggatgc
     9241 acggtccttt gcggcggcat gggaggcgtc atggaggaag ccgccagggg cgccgcctcc
     9301 gccgggggaa ccagcatcgg gctgcttccc cgcgaggctt cggaggcaaa cccctacctc
     9361 acccacgcga tcgcgacggg gatgggagaa gccaggaact ttttgatcac ccacgcctcc
     9421 ccggttctga tcgccatcgg gggaggactg ggaacgctct cggaaatcgc catggccctg
     9481 cggctgggca aacgggtcgt tgccctgcgg acctggacgg cccgcgacga ggaaaacaat
     9541 cccctgccgg tcctcccggc gcaaagcccg gaggaagcgg ttcggctggc tctcggggag
     9601 cccgggccat gagcggcgtc gagctgcgga acgtgacgaa ggcgttcgga aaggcggtgg
     9661 cggtccgcaa cctggacctt gccgtcggca agggagagtt cctggccctg ctggggcctt
     9721 ccgggtgcgg gaaaacgacc acgctgcgga tgatcgcggg tttcacccgc ccggattccg
     9781 gtgaaatcct cattgacggg cagcccgttt cttctctcag cgtccgggaa cggcgcgtgg
     9841 gaatcgtctt tcagagttac gccctgttcc cgaacatgac ggccttcgag aacatcgcct
     9901 tcggtctccg cgtgcggcgg atgcccgagg aggaggtacg gaggcgggtc tttcgcctgc
     9961 tggagatggt cggcctggag gacaaggcga acgccagctg ccgccagctt tccggcgggc
    10021 agcagcagcg ggtggccctg gcgcgggccc tggccatcga gccgcgggtc ctgcttttgg
    10081 acgagccgct ttcggccctg gacgcaaaag tccgaaacag cctgcggttc gagatcaaga
    10141 agatccagcg ggaaagcggc atcaccacga tctacgtcac ccacgaccag gaggaagccc
    10201 tctcgatttc cgaccgggtg gcggtgatgc gctacgggca gatcgaacag gccggaacgc
    10261 cctgggaggt ctactcgaac ccctccagcc tcttcgtggc ggatttcgtg ggcgtgagca
    10321 atttccttga cgtcgaggtg aaagccggcg gagcggtgtt ctggaacgac cgccccctgg
    10381 acgttgaagg cgccggatcc ctggctcccg gcccctggcg cctcgtgatc cgccccgaga
    10441 ggctgctgcc ggtcccgaag gacgcgcagg ggcaaaacat cctggaaggg accgtggccg
    10501 gccgggtgtt cctggggccg atccagcggg tggccctgga cgtgggaaaa gagcggattc
    10561 tcgtggacct cctgaacacg gagaaagggc acctctcgcc gggagagcct cttcgcgtct
    10621 cgttttcccc cggcgacgga cgcctgatcc atcgcagctg accttctccg ggcggggatg
    10681 cccggaactt tcaaaccgaa aaggggaggt gctgtcatga agagaaagtt catcgcgctg
    10741 atgtcgatcc tgcttttggc cgccttcgcc gtccaggcag cggccgccga gctgtacccg
    10801 ggagagaacg ccctgctcca gaaagcgaag aaggaaaagg ggctcaacag ctacgacacg
    10861 ggcccgacct gggcgaactg gcaggggctt ttcgacgcct tcaaggcccg ctacgggatc
    10921 aacatcacct acaacgacct gggcagcggg gccacggtgg tccgcctgga gaaggaaaag
    10981 aacaaccccc aggccgacac tgcctactac ttcatgccct ttggagcgct ggcgaaggaa
    11041 aagggcgtga cccagccctt caagcccgtg aacttcgaga agatcccgcc ggtgctgaag
    11101 gacaaggaag ggcactggtt ctgcatccac aaggggaccg tggtcttcgt ggtgaacaag
    11161 cgcctggtga aggagtcccc gaaatcgtgg aaggacctgc tggacaagaa gtacgccaag
    11221 tcggtcgtct acctggaccc caggacgacg gggatcggct atgccatcgt catcgccgca
    11281 gcctacgcca acgggggcag catcatcgac ctgtccaagg gagtggatta cctcgccgcc
    11341 ctccagaagg ccggcaacgt ccggatgatc gagaaaacca ccgagtacga caagttcctg
    11401 aagggggaaa tccccatctg gatcacgtat gactggaacg cctaccgggc caagtacatc
    11461 gccaacctcg gcgacgcggt ggaaatcatc ctgccgaaag acgggacgat cacggctccc
    11521 tacgccatca gcatggccaa gggggccccg aaccccaaca ccgccaagct ctggctgaac
    11581 ttcatcctca gcgaggaggg acagggcatc ttcgccaagg ggttcgtgag gccgatcctc
    11641 cccggggtga agctgccgcc ggaagttgcc ggcaagttcc tcccggcctc ggagtacgag
    11701 cgggcctacg acgtggactg ggtcctggcc cagaaggtcc aggaggacgc ggcgaagatg
    11761 tggggccgcc gcgtactggg cgagtagcga acggaacggg aggaaacgat ggagcggcgg
    11821 ggctggaagt tcctccttct tctgcctctg gcggcggtcc tgggaatttt cctggtcctg
    11881 ccgctccttt tcgtgctgta tgacagcttc tgggccgcgg gccgcctgtc tgcggccaat
    11941 tacctcctgg tcttccggaa gggcctttac agcgaggccc tgaggaccag cctggtgctt
    12001 tcggtctcga cggcgttcat cggagcggcc ctcggcctgc cgctctccta cctggtgcat
    12061 cgaagccggg ctccctggcg agacctcctc cttgcgatca ccgcggttcc gctcactttc
    12121 agcggcctgg tcatcgggtt cgccttcatc gtcctgctgg gaacgagcgg attcatcacg
    12181 atgcttttag agcgcttctt cgggatcaac cccctggagt tctccgcctt cctgttcacc
    12241 tggaaggggc tggtggtggc gtacctctac ttcctcatcc cgagaatgat cctgaccatg
    12301 gtcgcggcct ggagcaacgc cgactggagc caggtggagg ccgcgatgag cctgggagcc
    12361 tccttcccca ccatcctcgt gaagatcctt ttcccgatgc tgggcccttc cctgctggcc
    12421 ggcagctccc tgctgtttgc cgtcagcatg ggggccttcg ggaccgcctt cgccctgacg
    12481 ggcacggcag tcaagatcct ccccctggtg atctacaacc agatttcgga tgtgacggtg
    12541 gaggtttcga gggcgaacgc cctggccatc gtcctgacgg tcgtgacaac gctcgtgatc
    12601 gtcctttacg agaggagctt cgcccggaag caggcctgac gcagccaggc tccatccctt
    12661 aaaatggggg catggcaaac acttcggcgt ttcccctcta cctgatcgtg gactggctcc
    12721 tcttcgcggc cctggtgctg gcgccgcttt tcatttcgct tgcccttcgg cggaggcgcc
    12781 ggtgatgccc gttcttttgc tgcagatcct ggtgttcgga gcctacaccc tggccctggt
    12841 cttcctggcc ggggaggcct tcaactggaa ggaagaccgg cgcctgttct acgtgggggg
    12901 caaccgcctc accctcgtgc ccatggtggg gaccttcgtg gccacctgga tgagcgccgc
    12961 gtccgtgctg gggtacacca tgtggctcta cgcggacggg tacgtggcct tcacgggaag
    13021 cgtgaacggc tggctgatcg gcctgctgtt catgcccttc atcgtccgga agctccgtgc
    13081 gagccgggcc ctctcgctgc cccagtggct cggcgacact ttcggcgacc gccgcctccg
    13141 gtccctttcc gccgtggcgc tgatcggggc ctacaccttc tacatggtga tccagtttcg
    13201 ggccttcggc accatcgtgg cgaccctcct ggacatcaag gtgatcgtcg cctccgccct
    13261 cgtgtacctc ttcgtgctct acacgacctt cggcggactc ccctccgtgg cccgaagcga
    13321 cacgttgaac ctggccctga tccttggcgg cgtcctgctg gcggcgggaa ccctattgaa
    13381 gcgggcggga tcgctggctg ccgtccatgc cgcgatcgcc gagcgcgccc cggcgatgct
    13441 ttctccgtgg tcggaaggct ggagcccgct ggtgaccacc ctgatgatgc tttcgtgggg
    13501 gctcggggtc gccgccaacc cgcaatacgc cgtccgcctc ctttcggccc ggacgacccg
    13561 ggacgcgtac gggacgctgg ccacatcgtc cctgatcatc ggggccatct acgcggggct
    13621 caccgtgctg gggctttctg ccttcgtcct gttcccaggg gtgagggccg ccagcgacga
    13681 gctggccttc acgcggcttt tcagcctggc cctgcccccc ctgccggccc tggcgctttt
    13741 tttggcggtg ctggcggccg ccgtgagcac cgcgaactcg cagcttttgc tggcggcctg
    13801 ctccctctgc tacgacctga gcccgaggga gggaaggaac cggggagacc tcgtcacgga
    13861 ggaccggttc ctgttcaaaa accgcctggc catcgccggg atcgccacgg ccgccctggt
    13921 cctgagccag tttcggctgc cgggcgtgct tttgatcggc cgctacagct ggacggtggt
    13981 ggccatctgc ttctttcttc ccctttacgt gattcgaacc cccaggccga gggccctttt
    14041 cgttccgctt tccgcggctc ttttgcttca cctgttcctg acggtcttcc tgggcctcct
    14101 tcccgaactg gcccttcttc cagccctcgc cctcgaggga ttgctgtatt tcgggccctg
    14161 gaggaaaaaa tcatgagaag cccgcgtccg ctgagcattg agacccggat caaggtgatc
    14221 ctcaccgcca tcgccatggg cctgacggga ctgaccgtgg gaacggcgct gacacagggc
    14281 tttttcgagc gaaccggaga agcccggctg ctgttggaaa cggctcgctc tgcggccagc
    14341 gggcttcccg ccgcccaagt ggaacccctg cttgcgaggc tcaggccttt ctggaaggag
    14401 gctccgcggg cgcagggagc ggagacggtc cgctcggccg acgggcagga gtggcgcttt
    14461 gtcctggacc gggccgcgat ctggaacgcc ctggtctacc ggcagcggta cgtcctcgtg
    14521 gcgggactga tcgggcttct ggtggcagtc gagctggcgg tctttttggc gtacagcctg
    14581 acccgcccca tgaaaatcct ggcctgggga tgcgagcaga tgcgaagcgg ccgctgggtg
    14641 cggctgccga ccctctcccg gacgccctac gaacttgaag ccctgaacga ggcctttaac
    14701 ggcatggttg aggacctgaa gcggtggcag gacttccaga agcaggccgc ccgggtggag
    14761 cgcctcgcgg ccctgggctc
//
LOCUS       DAOOVN010000024        14502 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_2773_length_14502_cov_14.461549, whole genome shotgun
            sequence.
ACCESSION   DAOOVN010000024 DAOOVN010000000
VERSION     DAOOVN010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 14502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14502
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2773_length_14502_cov_14.461549"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..659
                     /locus_tag="PLY83_03665"
     CDS             57..659
                     /locus_tag="PLY83_03665"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOV29168.1"
                     /translation="MTNNPAERQAELESDDSLMARIGRGDRNAFETLYRRWSRRVMAY
                     AYRSLSDRAESEDVVQETFLSVFRSASRYSPRERFGAFLFRIAGNAVRSRWRKKRPLP
                     VDCSGEEEEDNFFPSKTIETEWSLFEERERIGAALATLPEAQRNALLLAVIGGLSYRE
                     IALEEGVSEDAVAARICRARKALRKALSEGGFEEEATTDE"
     gene            652..1299
                     /locus_tag="PLY83_03670"
     CDS             652..1299
                     /locus_tag="PLY83_03670"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOV29169.1"
                     /translation="MSESCEKMEVLISKVMDGECSPQERKALKSHLAECLRCRETIRA
                     FGRVDSLLESGKDLPAPPSCPKISEPGVSSRFPKAAFLAAAAAVAAFAFLGGAWWGQQ
                     KAIRSLPPSYSRVASAALWEKAPDPLRREAALAALPLSEAVTGYQREVARLLRQKDVD
                     WERVRRLVEAIGALRTDMELLTLHVAFIEKEQGKTDPASAAWLDLLGLDDRGGSL"
     gene            1336..2130
                     /locus_tag="PLY83_03675"
     CDS             1336..2130
                     /locus_tag="PLY83_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29170.1"
                     /translation="MRRMTVFVTMLFGVLAALASGLPASGAEVPVPLPEEVLQQAMVQ
                     SMVQARLETQAAKRLAAQQAFLADAVLEADLEQDRIRSGEEKDPVRLQDRIRTRAHQV
                     GTALGTALASGVKAYGEQNAVQVMATVMSAVRAGMSVQAASDSAGALASSGYTLHEMS
                     RIMQHTRERVRQDAPGDRGAALALQVRLMAQKRTAVQAMVGALDGNGHGQGAGGGQGG
                     SQGSGSGTGGSQGSGGQGSSGQGSGGQGSGNGSGGPGGSGSGKGGK"
     gene            complement(2203..3471)
                     /locus_tag="PLY83_03680"
     CDS             complement(2203..3471)
                     /locus_tag="PLY83_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29171.1"
                     /translation="MANQGGSGLRSIGAYNGALFIGTEDLNVWRNPSPSAQTPLASPP
                     MMTIVEVMGRQVPVTISDVGGKEGWTLVGKKSSFPGVATGAETQGWGGLWDFVSYNGW
                     LYASMADPVNGFSLYKAKDPSITTTPTPVSEDIWAWTPIVASLDVNPGAQFPQGLGND
                     QNAALTMTVFNGYVYAGTFSNWKDLIAYAMNPSEEAMNTVREHWTPPQVYRFDAKDAW
                     EMIVGDNSGQAASPYFENPSLSGWRAGFYDNEAISQDNYSTNRYIWRMAVHDDRLFMG
                     TMDMMTILEVLAENSSGDTLPNLVFALRQANPNNPPGFDLYVTEDGVNIQPITRNGGF
                     GELFGGTGDRYNYGARTLVSASDGNLYLGTANPFKGCQVWRINIEEDQEPGGGGGGGG
                     GGCSAILPERFFLLFLVPLLLFLGVIRQVK"
     gene            complement(3522..4055)
                     /locus_tag="PLY83_03685"
     CDS             complement(3522..4055)
                     /locus_tag="PLY83_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29172.1"
                     /translation="MRRRIAWSAFALLVLLSAAVVGAATFNVEKWSGVTTLPVNPETV
                     DGLVNDPENSYAWAMAEYGDYLYVGTNRNYLRQMFETIYGEGPLPMGLDNVFPPKNDY
                     AGKIYRKRLDGSGGWELFYDSPRVTVLMVPNPASPDVYEVQPNKILDYGYRTMEVFNN
                     SLSSLPIPSLKVHTPAC"
     gene            complement(4223..4873)
                     /locus_tag="PLY83_03690"
     CDS             complement(4223..4873)
                     /locus_tag="PLY83_03690"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOV29173.1"
                     /translation="MFDPFGWFDFLPERTRHRLVSAFVIAVCAALLVLRVREWPHFLF
                     KPLWVAEVLIYFVFIWMYAVRVEPVDRSRGVREIVLPLVAGALPFAFLLFPVNRQVVS
                     TRERAMALFAAMTLFTSLTIWGMVTLRKAFSVTVEARTLVTGGPYRFIRHPIYTGEAL
                     TAAMVVILRLSPLNAAMFILFTALQLYRSRLEERKLLRVFPEYRQTVGSAWWFWKI"
     gene            complement(4874..5578)
                     /locus_tag="PLY83_03695"
     CDS             complement(4874..5578)
                     /locus_tag="PLY83_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV29174.1"
                     /translation="MLSVTRLNVHYGKIHAVRDASFRVGKGEIVSIVGANGAGKSTIM
                     WTLAGVLKPSSGEILFDGKPLPGKPHEIVRSGLALVPERRRLFAELTVQENLIMGAYL
                     RHDKAIEEDLEKNFALFPILKQRLKQYAGTLSGGEQQMLAIARAMMSSPRMILFDEPS
                     LGLAPIVVDTLMETILQLRENGMTVLLVEQNAGRALEISDRAYVLDVGHIAKEGPGKA
                     LLDDPEVRRAYLGELA"
     gene            complement(5571..6350)
                     /locus_tag="PLY83_03700"
     CDS             complement(5571..6350)
                     /locus_tag="PLY83_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV29175.1"
                     /translation="MSAALLEVRGLTKAFGGIRAVDDLSFSVAPDAITGIIGPNGAGK
                     TTVFNLVTGVYRPDAGSIDFEGRSLLGIRPDQIVGAGIARTFQNIRLFNRRTCLENVL
                     TPLLQKAPYSVPAALLRTPGVRRMDRELREKSRFLLAEMGLEKLADVQASALPYGLQR
                     KLEIARALAMEPRLLLLDEPAAGMNPEESRALSALIEEIRERFNLTILLIEHHMDVVM
                     RVCSHIVVMNFGALLAEGPPEKVRTDEKVLQAYLGKEYRNA"
     gene            complement(6331..7188)
                     /locus_tag="PLY83_03705"
     CDS             complement(6331..7188)
                     /locus_tag="PLY83_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOV29176.1"
                     /translation="MNYVLSIVTLAGINMIAVLGLSIFTGFTGLFSFGHAALVGIGAY
                     ASAILTYYYFVPFPIALVAGMAASALVSLVIGIPTLRARLRSDYFAIAILGFGEALRV
                     ILENLEITNGARGLPGIEPFTTLPIVAGCLAVSVWVASNFLRSRYGAACIAVREDPVA
                     AEMAGINLFRTRLLSLVVSAAFCGLSGGLLAHFLSFIQPVMFTLVQSTALLAAVIAGG
                     MGSISGPLIASFIFIAMPEALRVAQMWRLVAYGLVLVLIMLFRPQGLMGYQELTTFFR
                     RSGHERRSA"
     gene            complement(7193..8071)
                     /locus_tag="PLY83_03710"
     CDS             complement(7193..8071)
                     /locus_tag="PLY83_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOV29177.1"
                     /translation="MFLQTVVTGLSIGGIYALMAVGYSLVFSVLSFSNFAHGAVIMLG
                     AYIGLGLVTKLHFGLGLVVSGAMAGAGLIAIVNEKLAYSLLRKRKAPSLYLMISAMGC
                     AVFLENMVYATIGSRFYAFPEFFKQSVLCLFNGTCNIALLDLFAFGISILAILGLHLF
                     LIHTRTGIAIRAGVSDMTMCSLMGVNLDRLISIVFFLAGIFGGAAGVFLGIKYLVYPT
                     MGWVTNKAYIAAVIGGLGSLPGALLGGLILGVLETFVSTYVSSVMRDVFSFGLLIALL
                     VWRPNGLFGRDTGEKV"
     gene            complement(8148..9299)
                     /locus_tag="PLY83_03715"
     CDS             complement(8148..9299)
                     /locus_tag="PLY83_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOV29178.1"
                     /translation="MLSFVLVLVFCLALGGAALAADTIKIGHTVSLTGGASMWGQSEK
                     LALDLLIKKINAQGGVLGKKLELISYDNRNDAVEAVNVARRLVNDGVVAVIGPAQSGN
                     SIATAPVLENAKIPMVVTTATNPYVTIDKRTGKVRPFAFRPCFIDPFQGTVAAKFAYR
                     DLKARTAAILYDVGSDYGQWLAKYFEEAFTKEGGKVVAKEAFRSEELDYRAQLGKMRP
                     LNPDVIFIPTSQKEAAMAAKQARDLGIKARLMGTDNWGSPDLIELGGEAINGGYFVNL
                     TDLADPAIKNFVAEYRKAYGADPVLPNPVMAQDALLMIVDAIKRAKSTDGTKIAAALA
                     TTKNLQVTSGPLTIDPKTHDPLDKPAVIQKVDVATKSFPFVKKFYPGGK"
     gene            complement(9447..10280)
                     /gene="dapF"
                     /locus_tag="PLY83_03720"
     CDS             complement(9447..10280)
                     /gene="dapF"
                     /locus_tag="PLY83_03720"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOV29179.1"
                     /translation="MISFTKVHGNGNDFIVIENLDRRFETEELSRIASTLCRRKTGIG
                     ADGILVVEPSQRADLTMRIFNSDGSEGEMCGNGARCFARYAFENRLAGETMAFETLAG
                     TMRAEVKAPNVILDMGTIDCSKGLFDETVSLLGETFPLAALRVGVPHCAVFVPNLASR
                     SRQDLVLLGRTLRHDALRFPQGVNVNFVQDLGEGELLVTTYERGVEDLTDSCGTGSTM
                     SAIAAVKRWGLSSPVRVLNPGGVNIVHLDWENDGRFCSARLEGPTALVARGAVLEEAG
                     L"
     gene            complement(10277..11023)
                     /locus_tag="PLY83_03725"
     CDS             complement(10277..11023)
                     /locus_tag="PLY83_03725"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HOV29180.1"
                     /translation="MLSGPREQEYLILEILSEATTPLGAGAIRQALEDRGMLLSEATV
                     GRLLRELDHRGYTRKSGFQGRSLSPKGAERLESLREERGHAVSANILLEELKSQGIEK
                     ILDVLVARRAIERETARLAARMATPEEIDRLRDLLAKQEEALQRKESVAPFDAAFHRE
                     IARISRNAILAAAVDLIRQDVKLGPAFEYARKKAASTLGSDHKSIIDAIADHDEDRAE
                     KAMVMHVENTLRDVQRYLDANPELRKETRR"
     gene            complement(11139..12095)
                     /locus_tag="PLY83_03730"
     CDS             complement(11139..12095)
                     /locus_tag="PLY83_03730"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOV29181.1"
                     /translation="MLFGLLGMRFILGLSWMDSLYYVTTTLTTVGYNPPANLTDEGKL
                     FIVVFLAVGILVIGLTLGQLAEKVLNRQILDALGRRRDRRLEKLSGHWIVCGLGRVGL
                     SVAEAIAEENLTVVVLDIDESKLELAREKGWYALKADATHEENLRACAVERAAGLVVS
                     TSNDANNVYVTVSARSLNKSLRIIARANDPQSSGILYRVGADKVVNPLLAGASAMARA
                     AVKPAVSDFLEIVHISRQFDLDFYSITLASDSPLVGLSLSESPLRSRYNVLVVAVKTR
                     EEQMVYNPQGSHVFQEGDELVLLGPRAATPELRRIASGLGTV"
     gene            12237..13022
                     /gene="xth"
                     /locus_tag="PLY83_03735"
     CDS             12237..13022
                     /gene="xth"
                     /locus_tag="PLY83_03735"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOV29182.1"
                     /translation="MALFRVATFNVNSIRSRMPAVTRWLNDNRVDVLCLQETKVQDVE
                     FDPEPFLRLGYSVVFRGQKATSGVAIASLEPLSEVRIGFSDGEEPEDGPRLIRGRLGG
                     LFLINTYVPQGKALDHPDFAYKLRFFRRMRALLERECSPEEPVLWVGDLNVAPTDIDV
                     THPENKRDHVCFHEDVKRAFEAVLGWGLVDVFRKHRPGEGEFSFFDYRVKDSLARNIG
                     WRIDHVLATKCLADASVDCWVDRLPRSWEKPSDHTPVVAVFDR"
     gene            13019..13351
                     /locus_tag="PLY83_03740"
     CDS             13019..13351
                     /locus_tag="PLY83_03740"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOV29183.1"
                     /translation="MMHLLALAVSIGLTVLGQYFLKTGSLSKTEGLRLFLNPATLAGL
                     FSYGFASLFYIVALKKIPLSVAYPSVSLSYVAVVVLAHFVWGEPLGLRQGVALALIVS
                     GIALLYKA"
     gene            13366..14349
                     /gene="galE"
                     /locus_tag="PLY83_03745"
     CDS             13366..14349
                     /gene="galE"
                     /locus_tag="PLY83_03745"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOV29184.1"
                     /translation="MERILVCGGAGYIGSHMVKRLLDEGFDVTIFDSFETGHREALPE
                     GAAVFEGTLNNPADVNGVLEMKPFDAVFHFAARIQVGESVRQPSLYYGNNVAGTLNLL
                     QAMVARGIGRFVFSSSAAVYGTPLEIPIREDHPLAPINPYGWTKRMVEQILADFWRAY
                     GLASVSLRYFNACGADPEGSIGESHDPETHLIPNILKSALSNSSEPVLIFGDDYPTPD
                     GTCVRDYVHVCDLADAHLKALDSLRASPGVRAYNLGNGCGFSVKQVLEAAERVLGAKI
                     ERRIAPRREGDPAFLVADSTLALKELGWRPRFTSLEEILQTALRWHRRPRY"
BASE COUNT         2698 a         4341 c         4799 g         2664 t
ORIGIN      
        1 gacccccgtc aggaaagcgg gggtcttcgc gttttgtcta gcagggagga cgaaccgtga
       61 cgaacaatcc ggcggagcgg caagcggaac tggaaagcga cgacagcctc atggcccgga
      121 tcggcagggg ggaccgaaac gcgttcgaga ccctttaccg ccgctggtcc agacgggtga
      181 tggcttacgc ctaccgatcc ctttcggaca gggccgaatc cgaggatgtc gtccaggaga
      241 cgttcctctc ggtctttcga tcggcctccc ggtatagtcc tcgtgaacgg ttcggtgcct
      301 tcctgttccg gatcgccggg aacgcggtac gaagccggtg gcggaaaaaa aggccccttc
      361 ccgtggattg ttccggggaa gaggaagaag acaacttctt cccgtcaaag acgatcgaaa
      421 cggaatggtc ccttttcgag gaacgggaaa ggatcggggc tgccctggcg accctccccg
      481 aagcgcaacg gaacgccctg ttgctcgcgg tcatcggggg cctgagctac cgggagatcg
      541 ccctcgaaga gggggtatcg gaagacgcag tggccgcaag gatctgccgt gcccgaaagg
      601 cgttgagaaa ggccctttcg gagggcgggt tcgaagagga ggcaacaacc gatgagtgag
      661 tcctgcgaaa aaatggaagt gttgatttcc aaggtcatgg acggggaatg cagcccacag
      721 gagcggaaag ccctcaagtc gcacctggct gaatgcctcc gctgccggga aaccatccgg
      781 gcgttcggcc gggtggattc tctcctggag agcggaaagg acttgccggc ccctccgtcc
      841 tgtccgaaga tctccgaacc gggggtttcg tcacggtttc cgaaagcggc gttcctggcg
      901 gccgcggccg ccgtcgcggc cttcgctttc ctgggcggag cctggtgggg gcagcaaaaa
      961 gcgatccgaa gcctgccgcc ttcctacagc agggtcgcat cggcagccct gtgggaaaag
     1021 gccccggatc ccctccgccg ggaagcggcc ttggccgccc tccccctttc cgaggccgtg
     1081 accggctacc agagggaggt tgcccgtctc ctgcggcaga aagacgtgga ctgggaacgg
     1141 gtccgccgcc tggtcgaggc catcggggcc ctgagaacgg acatggaatt gctgacgctc
     1201 cacgtggcct tcatcgaaaa agaacagggg aaaacggatc cggccagcgc cgcctggttg
     1261 gacttgctgg gcctggacga tcgcggcgga tcgctctgag gtccgaaacc tgaacgcggg
     1321 agggaaaaga cgaagatgag aaggatgacg gtttttgtga cgatgctttt cggtgtcctg
     1381 gctgcattgg cgtcgggact gccggcttcg ggggcggaag tccccgtccc gttgccggaa
     1441 gaggtccttc agcaggcgat ggttcaatcg atggtgcagg cccgcctgga aacgcaggcg
     1501 gcaaagcgtc ttgcggcgca gcaggctttt ctggcggatg ccgtgctcga ggcggatctc
     1561 gagcaggacc ggatccgctc gggcgaggaa aaggaccccg ttcggctgca ggatcgcatc
     1621 cgaacccggg cgcaccaggt cggcaccgcc cttgggacgg ctttggcctc gggagtgaag
     1681 gcctacggcg aacagaacgc cgtacaggtg atggcgacag tcatgtccgc ggtccgggca
     1741 gggatgagcg tccaggcggc ctcggattca gccggggccc tcgcttcttc cgggtatacc
     1801 ctgcacgaaa tgagccgaat catgcagcac accagggaaa gggtccggca ggacgctccc
     1861 ggggacaggg gggccgccct ggctttgcag gtccggctca tggcacagaa gcggactgcc
     1921 gttcaggcca tggtgggcgc tcttgacggg aacggccacg ggcaaggcgc aggcgggggg
     1981 caaggcggaa gccagggttc cggaagcgga accggaggga gccagggttc cgggggccag
     2041 gggtctagtg gacaaggttc cgggggccag ggttccggga acggctctgg gggcccagga
     2101 ggttccggca gcggaaaggg cgggaaatag caaggcaggc aaagggcccg gtccccttca
     2161 gggggccggg cccttccacg cttcaggcgc tcgtgcgcga ggtcacttca cctgccggat
     2221 cacgccaagg aagagaagca gcggcacgag gaacagcagg aagaaccgtt cgggcaggat
     2281 ggccgaacag ccgccgcccc cgccgccccc gccccctccg ggttcctgat cctcttcgat
     2341 gtttatcctc cagacctggc agcccttgaa ggggttggcg gttccgaggt agaggttccc
     2401 gtcggaggcc gagacgaggg tccgggcccc gtagttgtac cggtctcccg tgccgccgaa
     2461 gagctccccg aagccgccgt tacgggtgat cggctggatg ttcaccccgt cctccgtgac
     2521 atagaggtcg aatccaggcg gattgttcgg gttggcctgg cggagggcga aaacaagatt
     2581 cggaagggtg tcgccgctgg aattctctgc gagcacttcc aggatggtca tcatgtccat
     2641 ggtccccatg aagagtcggt cgtcgtggac cgccatccgc cagatgtagc ggttcgtgga
     2701 gtagttgtcc tgcgagatgg cctcgttgtc gtagaacccg gcccgccacc cgctcagcga
     2761 cgggttttcg aaataggggc tggccgcctg tccggagttg tcaccgacga tcatctccca
     2821 ggcgtctttc gcgtcgaagc ggtagacctg cggaggcgtc cagtgctccc ttaccgtgtt
     2881 catcgcctct tctgagggat tcatggcgta ggcgatcagg tccttccagt tggagaaggt
     2941 gccggcgtag acgtacccat tgaagacggt catcgtgaga gcggcgttct ggtcgttgcc
     3001 gaggccctgg gggaactggg ccccggggtt gacgtccagg ctcgccacga tgggcgtcca
     3061 ggcccagatg tcttccgaaa cgggagttgg cgttgttgtg attgaggggt ctttggcttt
     3121 ataaagagag aagccgttca ccgggtcggc catcgaggcg tagagccacc cattgtagct
     3181 cacgaaatcc cacaggccgc cccacccctg ggtctcggcc cctgtcgcca cccctgggaa
     3241 gctggacttc tttccgacga gggtccagcc ctccttgccg cctacgtcgc tgatcgtgac
     3301 gggaacctga cgacccatga cttccacgat cgtcatcatg ggcggagaag caagcggggt
     3361 ttgggctgac gggctcgggt tcctccagac gttcaggtcc tctgtcccga tgaagagggc
     3421 tccgttgtaa gccccgatgg agcgcaggcc tgaccccccc tggttggcca cccggagaac
     3481 ctcctggagg tcggcgcttg ttcccgtcgg ggtcggtagt ttcagcacgc gggagtatgg
     3541 actttcaaag aaggaatagg taacgatgaa agagaattgt tgaagacctc catcgttcgg
     3601 tagccgtagt ccaggatctt gttcggctgc acttcataga catcgggact ggccgggttg
     3661 ggcaccatga ggacagtaac cctcggcgaa tcgtaaaaga gttcccaccc tccgcttccg
     3721 tcgagccgct tgcggtagat cttgcccgcg tagtcgttct tgggggggaa gacgttgtcc
     3781 agccccatgg gcagcgggcc ttcaccgtag atcgtctcga acatctgcct caaataattg
     3841 cggttcgttc ccacgtaaag gtaatccccg tattctgcca tggcccaggc gtagctgttt
     3901 tccgggtcgt tgaccaggcc gtcgaccgtt tcagggttca ccggcagggt cgtgacgccg
     3961 ctccatttct cgacgttgaa cgtcgcagcc cccaccacgg cggccgacaa cagcactaga
     4021 agtgcaaaag cactccaggc aattcgcctt cgcatgtctc cacgctcctt tcagcccgat
     4081 ttttgtcgcc ttgaccggaa gaaaaaacgg aaggaggcgg caacgaaagc ataacatctt
     4141 aatgtctatt tatcaagcca aagaaatgat aaataaaata gtacacattg tccgcaaaag
     4201 gcgggaggac ctttaggagc cttcagattt tccagaacca ccaggccgag cccacggttt
     4261 gacggtattc ggggaaaacc cgcaggagtt tccgctcttc caggcgggag cggtaaagct
     4321 ggagggccgt gaagaggata aacatggcgg cgttgagagg ggaaagcctc aggatcacga
     4381 ccatggcggc cgttagggct tcccccgtgt agatggggtg ccggatgaac cggtaggggc
     4441 ccccggtgac gagggtccgg gcctccacgg tgacgctgaa ggctttccgg agcgtcacca
     4501 ttccccagat cgtcaacgag gtgaaaaggg tcatggccgc aaacagggcc atggccctct
     4561 cccgggtcga gacgacctgg cggttcacgg ggaaaaggag gaaggcgaag ggcagggcgc
     4621 cggctacgag aggaaggacg atttcccgca ctcccctgga gcggtcgaca ggctcgaccc
     4681 gcacggcgta catccagata aagacgaaat agatgagcac ctcggcgacc cagaggggct
     4741 tgaaaagaaa gtgcggccac tcccggaccc gcaggaccag gagggccgcg caaacggcga
     4801 tcacgaaggc cgaaaccagg cggtggcggg ttcgctccgg caggaaatcg aaccagccga
     4861 aggggtcgaa caactaggcc agctccccca ggtaggcgcg ccggacttcc gggtcgtcca
     4921 ggagggcttt ccccggccct tccttggcga tgtgcccgac gtcgaggacg taggcccggt
     4981 cggaaatttc cagggctcgc cccgcgttct gctccaccag caggacggtc atgccgttct
     5041 cccgcagctg gaggatcgtt tccatgagcg tgtccaccac gatcggggcc agtcccaggg
     5101 aaggctcgtc aaagaggatc atgcgcgggg agctcatcat ggcgcgggcg atggccagca
     5161 tctgctgttc tcccccggaa agggtcccgg cgtactgttt caggcgctgc ttcaggatcg
     5221 ggaaaagggc gaaattcttc tccaggtcct cctcgatggc cttgtcgtgc cggaggtacg
     5281 cccccatgat gaggttttcc tgcaccgtca gctcggcaaa cagccgtctc cgctccggga
     5341 cgagggccag gccgctccgg acgatctcgt ggggtttccc gggcagcggt ttcccgtcaa
     5401 agaggatttc tcccgaggaa ggtttcagga cgccggccag ggtccacatg atcgtcgatt
     5461 tcccggcccc gttggcgccg acgatggaga cgatctcgcc ctttcccacc cggaagctgg
     5521 catcccggac ggcatggatc ttcccgtagt gaacgttgag cctcgtcacg ctaagcattc
     5581 cggtactcct tccccaggta ggcctggagc actttttcgt cggtgcgcac cttctccggc
     5641 ggtccttcgg ccagcagcgc cccgaaattc atgaccacga tgtgggaaca gacccgcatg
     5701 accacgtcca tgtgatgctc gatcaggagg atggtcaggt tgaagcgttc ccggatttcc
     5761 tcgatcaggg ccgataaggc ccgggactcc tccgggttca tccccgccgc gggttcgtcc
     5821 agcagaagca gccgaggttc catggccagc gccctggcga tctcgagctt tctctgcagg
     5881 ccgtacggca gggccgatgc ctggacgtcc gccagtttct cgaggcccat ctcggccagg
     5941 agaaagcgtg atttttcccg gagctcgcgg tccatccgcc ggactccagg ggttctcagg
     6001 agggccgcgg gaacgctgta gggggctttc tggagcagcg gggtgagcac gttttccagg
     6061 caggtcctcc ggttgaacag gcggatgttc tggaaggttc ttgcgatccc ggccccgacg
     6121 atctggtccg ggcggatgcc cagaagcgac cgtccctcga agtcgatgct gccggcatcg
     6181 ggccggtaaa cccccgtcac gaggttgaag acggtggtct ttcccgcgcc gttggggccg
     6241 atgatgccgg tgatggcatc gggggcgacg gaaaaagaca ggtcgtccac cgcccggatg
     6301 cctccgaacg ctttcgtgag tcccctcacc tcaagcagag cggcgctcat gtccggacct
     6361 cctgaagaag gtggtgagtt cctggtagcc catcagcccc tggggccgga agagcatgat
     6421 caaaacgagc acgagcccgt aagccaccag gcgccacatc tgggccacgc gcagggcctc
     6481 gggcatggcg atgaagatga acgaggcgat gaggggcccc gagatgctgc ccatgcctcc
     6541 ggcgatgacc gcggcgagaa gcgcggtgga ctgaaccagc gtgaacatga cgggctggat
     6601 gaacgaaagg aagtgtgcga gaaggcctcc cgagagcccg cagaaagcgg cgctcaccac
     6661 cagcgagagc agccgggtac ggaacaggtt gatccccgcc atctccgccg caacgggatc
     6721 ctcccggact gcgatgcagg ccgctccgta ccggctgcgg aggaagttcg acgcgaccca
     6781 gacggaaacg gccaggcacc ccgcgacgat gggcagcgtc gtgaagggct cgatgccggg
     6841 cagtccgcgg gcgccgttgg tgatctcgag gttctccagg atgacccgca gcgcctctcc
     6901 gaagcccagg atggcgatgg cgaagtagtc gctccgaagc cgggccctca gggtcgggat
     6961 cccgatgacg aggctgacca gggccgaggc ggccatgccg gcaaccaggg cgatcgggaa
     7021 ggggacgaag tagtagtagg tcaggatggc cgaggcgtag gccccgatgc ccaccagggc
     7081 cgcgtggccg aacgagaaca ggccggtgaa ccccgtgaag atcgaaaggc ccagcacggc
     7141 gatcatgttg attcccgcga gagtgacgat ggaaaggacg taattcatcg gatcacacct
     7201 tctctcccgt gtctcggccg aagaggccgt tggggcgcca gaccaggagg gcgatcagca
     7261 ggccgaagct gaagacgtcc cgcatgacgc tggacacgta ggtggaaacg aaggtttcga
     7321 gcacccccag gatcaggccg cccagaagcg cccctggaag gctccccagt cccccgatga
     7381 cggcagcgat gtaggccttg ttggtgaccc atcccatcgt cgggtagacg aggtacttga
     7441 tgccgagaaa gacccccgct gctcccccga agatccccgc caggaagaag acgatggaga
     7501 tgagacggtc caggttgacg cccatgaggg aacacatggt catgtcggaa acccccgccc
     7561 ggatggcaat tccggtgcgc gtgtggatca gaaagagatg aagcccgagg atcgccagga
     7621 tggagatgcc gaaggcaaac aggtcgagca gggcgatgtt gcaggtgccg ttgaagaggc
     7681 aaaggacgct ctgcttgaag aactcgggaa acgcgtagaa acgggatccg atggtggcgt
     7741 agaccatgtt ttccaggaac acggcacagc ccatggccga gatcatgagg tagagggacg
     7801 gggccttgcg cttgcgaagg aggctgtagg cgagcttttc gttgacgatg gcgataagcc
     7861 cggctcccgc catggctccg gagaccacga gcccaaggcc gaagtgaagc ttcgtgacga
     7921 ggcccaggcc gatgtaggcc ccgagcatga tgacggctcc gtgggcgaag ttgctgaagg
     7981 aaaggacgct gaagaccagg gagtagccga cggccatgag cgcgtagatc cccccgattg
     8041 aaagccccgt gacgacggtc tgcaggaaca tggggtccct cctatcttgc tttcccagga
     8101 acaggggagg ccccgccctc catgagcgga gcctcctcag gaaacaccta cttgccgccc
     8161 ggatagaact tcttcacgaa cgggaaggac ttggtggcca cgtcgacttt ctggatgacg
     8221 gccggcttgt ccagcgggtc gtgggtcttc gggtcgatgg tcaaaggacc gctcgtgacc
     8281 tgcaggttct tggtcgtggc gagggccgcg gcgatcttgg tgccgtccgt gctcttggcg
     8341 cgcttgatcg cgtccacgat catgagcagc gcgtcctggg ccatgacggg gttcggaagg
     8401 acggggtcgg caccgtacgc cttgcggtat tcggccacga agttcttgat ggccgggtcg
     8461 gccaggtcgg tcaggttgac gaaatagcct ccgttgatcg cctccccgcc aagctcgatg
     8521 aggtcagggc tgccccagtt gtcggtgccc atcaggcggg ccttgatgcc gaggtctctc
     8581 gcctgcttgg cggccatggc cgcctccttc tggctggtgg ggatgaagat cacgtcagga
     8641 ttcagcgggc gcatcttgcc cagctgggcg cggtagtcca gctcctccga gcggaaggcc
     8701 tccttggcca cgaccttgcc gccttccttc gtgaaggcct cctcgaagta cttcgccagc
     8761 cactgcccgt agtcggaacc gacgtcatag aggatggcgg cggtcctggc cttcaggtcc
     8821 cggtaggcaa acttcgcggc gaccgttccc tggaaggggt cgatgaagca gggccggaag
     8881 gcgaaggggc gaaccttgcc ggttctcttg tcgatcgtga cgtaggggtt ggtggcggtg
     8941 gtgacgacca tgggaatctt cgcgttttcg aggaccggcg ccgtggcgat ggagttgccg
     9001 ctctgtgccg ggccgatgac ggcgaccacg ccgtcgttga cgaggcggcg ggccacgttg
     9061 acggcctcga ccgcgtcgtt gcggttatcg tagctgatca gctcgagctt tttccccagg
     9121 accccgccct gtgcgttgat cttcttgatc agaaggtcca gggccagctt ttccgattgc
     9181 ccccacatgg aggccccgcc ggtaagggag accgtgtgtc caatcttgat ggtgtccgcc
     9241 gccagggcgg ctccgccaag ggcgaggcag aaaaccagga ccagaacgaa agacagcatt
     9301 cttctcactg ggcattcctc cttctcttcc acgggtatgg gtttccggac tcctggacag
     9361 ggcggcccct ccggaaggga tcccgccccg gcgcgcaggt tgctgccaag ttcaaaagaa
     9421 ggtccgtttt ccaggggctc cctcccttac aatccggcct cttccaggac cgccccccgg
     9481 gccaccagcg cggtgggccc ttcaaggcgg gcactgcaga agcggccgtc attctcccag
     9541 tccaggtgaa cgatgttcac gccgcccggg ttcaggaccc ggaccggcga ggacagcccc
     9601 cagcgtttca cggcggcgat ggcgctcatg gtggacccgg ttccgcagga atccgtcagg
     9661 tcctcaacgc cgcgttcgta ggtcgtcacg aggagttccc cttctcccag gtcctggacg
     9721 aagttgacgt tgaccccctg ggggaagcgc aaggcgtcgt ggcggagcgt tcgcccgagg
     9781 agcaccaggt cctggcggga ccgcgacgcg aggttgggaa cgaagacggc gcagtggggc
     9841 acgccgaccc tcagggccgc cagcgggaac gtttccccga gaagcgaaac cgtttcgtca
     9901 aagaggccct tcgagcagtc gatcgttccc atgtccagga tcacgttggg ggccttcacc
     9961 tcggcgcgca tggtccccgc gagggtttcg aaagccatgg tttcgccggc gaggcggttt
    10021 tcaaaggcgt accgggcgaa acaccgagcc ccgtttccgc acatttcccc ctcggatccg
    10081 tcgctgttga agatcctcat cgtaaggtcc gccctttgag agggttcgac cacgaggatg
    10141 ccgtcggccc cgatgccggt ttttctccgg cagagcgtgc tcgcgatccg ggaaagctct
    10201 tccgtctcga accggcggtc gaggttttcg atgacgatga aatcgtttcc gttcccgtga
    10261 accttggtga aggaaatcat cggcgggtct ccttcctgag ctcgggattc gcgtcgaggt
    10321 aacgctggac gtcccgaagg gtgttttcca cgtgcatcac catggccttc tccgcccggt
    10381 cctcgtcgtg gtccgcgatg gcgtcgatga tggacttgtg gtccgagccc agcgtgctgg
    10441 ccgctttctt gcgggcgtac tcgaaggcgg ggccgagctt cacgtcctgg cggatgaggt
    10501 cgacggctgc agcgagaatg gcgttgcggg agatgcgggc aatttcccgg tgaaaggccg
    10561 cgtcgaaggg ggcaacgctt tcttttcttt gaagagcttc ttcctgcttg gccagcaggt
    10621 cgcggaggcg gtcgatctcc tcgggggtag ccatgcgggc cgccaggcgg gccgtttccc
    10681 gttcgatggc gcgcctggcc acgaggacgt ccaggatttt ctcgatgccc tggcttttca
    10741 gttcttccag gaggatattg gccgaaacgg catgcccccg ctcctcgcgg agggattcaa
    10801 gacgttcggc gcccttgggg gaaaggcttc gtccctggaa gccggatttt cgcgtgtatc
    10861 cccggtggtc gagttcgcgg agcaatcggc ccaccgtggc ttcgctcagg agcatgcccc
    10921 ggtcttcgag ggcctggcgg atggcgccgg ctccaagggg agtggtagct tccgaaagga
    10981 tctcgaggat cagatactcc tgttccctgg gtcccgacag cacgacggca cctccctgcc
    11041 aatatttatg gctcatcaat gatgagccat aaatattgta accctcgaag gggggtgcgt
    11101 caatcggatg gaaagaaaaa aggttgctct gctttaggtt aaacggttcc gaggccgctg
    11161 gcgatccttc tcagttccgg agtcgcagcg cggggaccca gcagcaccag ctcgtcgccc
    11221 tcctggaaga cgtgactgcc ctggggattg tagaccattt gttcttcccg ggtcttcacg
    11281 gccacgacca ggacattgta ccgcgaacga agggggcttt cggagaggga aagtccgaca
    11341 aggggactgt cggaagccag ggtgatggaa tagaaatcga ggtcgaactg gcgggaaatg
    11401 tgaacgatct ccaggaaatc cgacaccgcg ggcttgacgg ccgcccgtgc catcgcgctg
    11461 gcgcccgcca ggagggggtt cacgaccttg tcggctccga cccggtagag gatcccggaa
    11521 gattgggggt cgttggcccg tgcaatgatg cgcaggctct tgttcaggga acgcgccgaa
    11581 acggtgacgt agacgttgtt ggcatcgttc gaggttgaaa cgaccaaccc ggcggcgcgc
    11641 tccaccgcac acgcgcgaag gttttcttcg tgcgtggcgt ccgccttgag ggcgtaccac
    11701 cctttttccc ttgccagttc aagcttcgac tcgtcgatgt ccaggaccac gacggtcagg
    11761 ttttcttcgg cgatggcttc tgcgacgctc aagcccacgc gcccaagccc gcagacgatc
    11821 cagtggcccg atagtttttc caagcggcgg tccctccttc tgcccagggc gtccaggatc
    11881 tggcgattca ggactttttc cgcgagctgt cccagggtga gcccgatcac caggattcca
    11941 acggcaagga agacgacgat aaacagcttt ccttcatcgg tcaggttcgc cggcgggttg
    12001 tacccgacgg tcgtcagggt ggtcgtcacg tagtacaggg aatccatcca ggaaagtccc
    12061 aggatgaacc gcatgcccag caggccgaaa aggatgaccg aaacgaaggg gaggagccag
    12121 aggggaaacg gtttccgctt tttccggatg gcaatccctt ccttcaggga gactccttgt
    12181 cattccaggg acatcccata caatgggcac aaagaggatg gaggaggagt gtcccgatgg
    12241 cccttttccg cgtcgcaacg ttcaacgtga attcgatccg gagccgcatg cctgcggtga
    12301 cgcgctggct caatgataac agagtggacg tgctgtgtct ccaggaaacg aaggtccagg
    12361 acgttgagtt tgaccccgag ccgttccttc ggctgggata ttccgtcgtt tttcggggcc
    12421 agaaggcgac gagcggcgtg gccatcgcct ccctcgaacc gctgtcggag gtgcggatcg
    12481 gtttcagcga cggggaagag ccggaagatg gaccccgcct gattcgtgga cggctgggag
    12541 gactgttcct gatcaacacc tacgtccccc aggggaaggc cctggaccat ccggactttg
    12601 cctacaagct ccgtttcttc cggcggatgc gcgctctcct ggagagggag tgctccccgg
    12661 aggaaccggt cctctgggtc ggggacctca acgtggcccc cacggacatc gacgtgaccc
    12721 atcccgagaa caagcgcgat cacgtctgtt tccacgagga cgtgaagcgg gcgttcgagg
    12781 cggttctggg gtgggggctg gtggacgtgt tccgcaagca ccgtcccggg gaaggggagt
    12841 tttccttctt cgactaccgg gtcaaggatt ccctggcccg gaacatcggc tggcgcatcg
    12901 atcacgtcct ggccacgaaa tgcctggcgg acgcctccgt ggactgttgg gtggaccgcc
    12961 tcccgcgctc ctgggaaaaa ccctcggacc acaccccggt cgtggcggtc ttcgaccgat
    13021 gatgcacctc ttggccctgg cggtctccat cggactgacg gtcctggggc agtatttcct
    13081 gaaaaccgga tccctctcga aaaccgaggg actccgcctt ttcctgaacc ctgccaccct
    13141 tgcggggctg ttctcctacg gctttgcctc gctcttctat atcgtggccc tgaagaaaat
    13201 ccccctttcg gtcgcctatc ccagcgtgtc gctgagctac gtcgcggtcg ttgtcctggc
    13261 tcacttcgtc tggggggagc cgctgggcct gcggcaaggg gtagctctag ccctgatcgt
    13321 ttcgggaatc gctcttctct acaaggcata ggaggtctgg ctcgaatgga acggatcctg
    13381 gtttgcggag gtgcggggta tatcggctcg cacatggtga agcgcctgct ggatgaagga
    13441 tttgacgtga cgatcttcga cagcttcgaa acgggtcacc gggaagcgtt gccggaagga
    13501 gccgcggttt tcgagggaac cctgaacaac cccgccgatg tgaacggcgt gctcgagatg
    13561 aagcccttcg atgcggtctt ccactttgcg gcccggatcc aggtggggga atcggtcagg
    13621 cagccgtccc tgtactacgg gaacaacgtc gcggggacct tgaacctcct gcaggcgatg
    13681 gtggcgcgag ggatcgggag gttcgtcttt tcgtcctctg cggcggtcta cggcacgccc
    13741 ctggaaattc cgatccggga ggatcacccc ctggccccca tcaaccccta cggctggacc
    13801 aagcggatgg tcgagcagat cctggccgat ttctggcggg cttacggcct ggcctcggtt
    13861 tcgctccgct acttcaacgc ctgcggggcc gacccggagg gaagcatcgg ggaaagccac
    13921 gatcccgaga cgcacctcat cccgaacatc ctgaagagtg ccctctcgaa ttcgtcggaa
    13981 ccggtactga ttttcgggga cgactatccc accccggacg gaacctgcgt gagggattac
    14041 gtccatgtgt gtgacctggc ggatgcgcac ctgaaggccc tggattccct ccgggcctcg
    14101 cccggtgttc gcgcgtacaa cctgggaaac gggtgcgggt tttccgtgaa gcaagtgcta
    14161 gaggcggcag agcgtgtcct gggcgcgaaa atcgaacgcc ggatcgcccc ccggagggaa
    14221 ggcgacccgg catttctcgt ggcagacagc accctggcgc tgaaggaact cggctggcgt
    14281 ccccgcttca cctcgctgga ggagatcctt cagacggctt tgcgctggca ccgccgccct
    14341 cgctactaga aggggcggtc agatctcggc cagggtcagg tgttcccgga accggtccag
    14401 gtatttcgag aacgccttga agtaccggga agaaggggct cccaggtatc gagccagggc
    14461 cttcttctcc gtctggtggg ccttcatgta gcgcgacagg ag
//
LOCUS       DAOOVN010000025        14129 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_2879_length_14129_cov_13.282365, whole genome shotgun
            sequence.
ACCESSION   DAOOVN010000025 DAOOVN010000000
VERSION     DAOOVN010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 14129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14129
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2879_length_14129_cov_13.282365"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="PLY83_03750"
     CDS             complement(<1..817)
                     /locus_tag="PLY83_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV29185.1"
                     /translation="MSLREIVVVSGKGGTGKTTLTSVLARALSGNAVLCDADVDVPDL
                     WILLKPSVHSEEEFVGGNKAALLRPPCDDCGLCVPVCRFGALSSENGRVILDRSRCEG
                     CGACTLLCPRGAFSLSPAVQGRIYGSETDFGPFWHARLVPGGENSGRLVQLLREKARA
                     SALEQGKSWILVDGPPGIACPAISAMTGCSIALAVTEPSKSGLHDLKRLGELAKRFRV
                     PMAVVLNRSDLSAEGARAVRQACREAGWPLVAEIPFLVGVVEDLARARIPEAIP"
     gene            complement(814..>1632)
                     /locus_tag="PLY83_03755"
     CDS             complement(814..>1632)
                     /locus_tag="PLY83_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV29186.1"
                     /translation="SGKGGTGKTSFSASLALSGQPVAAVDADVEEPNLALLLGDPVLA
                     TMNVSLPVPAVDAERCTRCGTCSRTCRFGAVFSFGKAVPVFNDLCHGCGACVLACPEK
                     ALHEVERPVGFVGRGDANGIVVVEGRLLVGMPNPVPVIERTVELGLAEGLDTVIDSPP
                     GAACPMVAAVRKARAVILVTEPTPFGRADAEVVAQVLREMGKPAALVVNRSGILEEDP
                     PFEGVLGTLPVLARLPFSRAVAEGAARGIPPTEVDSSWKEAAGAAWEWARRVAK"
     assembly_gap    1633..1732
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1733..2007)
                     /locus_tag="PLY83_03760"
     CDS             complement(<1733..2007)
                     /locus_tag="PLY83_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOV29187.1"
                     /translation="MKIAIPLEGGRVCAHFGHAPHFLVAVTEEGHVVKKEMHENPGHE
                     PGKLPAWLAGLGVKAILAGGMGERAISLFQAQGVQVCVGVSGPAEEAL"
     gene            complement(2027..2257)
                     /locus_tag="PLY83_03765"
     CDS             complement(2027..2257)
                     /locus_tag="PLY83_03765"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 family protein"
                     /protein_id="HOV29188.1"
                     /translation="MPGMDRTGPRGVGPLTGWGRGPCGGGRSFGRSLGRGFGAWRQWN
                     SGEPLSGTGLWARLEALEEEVARLRENPKAEE"
     gene            2371..2820
                     /locus_tag="PLY83_03770"
     CDS             2371..2820
                     /locus_tag="PLY83_03770"
                     /inference="COORDINATES: protein motif:HMM:NF020400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGGC domain-containing protein"
                     /protein_id="HOV29189.1"
                     /translation="MERELVKVGIFICNRYHTCAGGKCLRALKNREGAFSLYEGKDVE
                     LVGYTTCDGCPGGNIEYAPAEMKKNGATVIHLATGMLVGYPPCPRIDFFRRFIRENYD
                     LPVVVGTHPIPEKYFRIHEALGTWNSPLWQELIAPTLATPEIRLAYN"
     gene            complement(2861..3706)
                     /locus_tag="PLY83_03775"
     CDS             complement(2861..3706)
                     /locus_tag="PLY83_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOV29190.1"
                     /translation="MSENACESCSQKGTCNSTPETCGLPPKSDKGRIRRIVAVGSGKG
                     GVGKSSVSALLAIGLARKGLKVGVLDADITGPSIPKLLGVRALPEGSPLGIVPPQSPV
                     LGISVMSINLLLDDVTKPVVWRGPIIANTVKQFYEEVFWGDLDTLIVDLPPGTSDAPL
                     TVMQSLPLDGMVVVTSPQELAGMVVEKAMNMARMLNVPILGLVENMAYALCPHCGERW
                     DLFGPSHLDKLATKWSVAKLASLPMDAFISRLGDKGRLEEYDNPGLLDPLVEAFCAVE
                     ATVTE"
     gene            complement(3748..4074)
                     /locus_tag="PLY83_03780"
     CDS             complement(3748..4074)
                     /locus_tag="PLY83_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOV29191.1"
                     /translation="MNIAIAAEGPSPQARPSDRFARAPWFLLFRSDGTFLEAVSSKAG
                     EHGAAAAAVSLLAQKDVGAVLAPRLGPNAVSALAAANLKAYLSEGETAEEGVRLFLAG
                     KAKVLD"
     gene            complement(4179..4706)
                     /locus_tag="PLY83_03785"
     CDS             complement(4179..4706)
                     /locus_tag="PLY83_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOV29192.1"
                     /translation="MTETDRKDLRQRAEEYLEALRRKGCRRTEQRRLIIETLLESQGQ
                     HLNARELMERVQSKDPTVGFATVYRTLLVLGETGLVHSFDQGEGFARFDIPQETMHFH
                     VLCRSCGKMVHLEDEAAKQEIVAAWIGEAGFRIVPQTFQFYGICPDCLEKGAPMDKAA
                     WAETCCGRKRLGWNR"
     gene            complement(4699..5142)
                     /locus_tag="PLY83_03790"
     CDS             complement(4699..5142)
                     /locus_tag="PLY83_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HOV29193.1"
                     /translation="MAKVVAVCTSKDRRKPKEPQAEAMLVRGKGIEGDSHFGLTERQV
                     SLLRSEDICEAERSAGFPFPPGSLAENLVIEGLPEDLLPGQGLAIGPDVRLRVVEKGK
                     KAGEPHTYDYRGWCLLPTKGFFLSVERGGTVVPGMEVVLEGEARD"
     gene            5253..5564
                     /locus_tag="PLY83_03795"
     CDS             5253..5564
                     /locus_tag="PLY83_03795"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOV29194.1"
                     /translation="MRKGWLLFILALALAAGALATTAAGPALAADKLLVCGICDKSFY
                     ETAVKVVQEMGLADRVTVKKSSCLGACSQPPVVEFKGTLYTSMTAEKLKALLAKTYSG
                     S"
     gene            complement(5549..7654)
                     /locus_tag="PLY83_03800"
     CDS             complement(5549..7654)
                     /locus_tag="PLY83_03800"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOV29195.1"
                     /translation="MNLLIPFDRLSEKDRPRAGGKAWALSRLGREGFPVPRGAVVPVE
                     AYRAYVGPTGLRERILLELNRKPFEAMRWEEMWDAALRIRNFFLKTPLPESLRAALLA
                     EIEELFGNRPVAVRSSAPGEDSAGASFAGLHESFLNVRGASAILERIRLVWASLWSDG
                     ALLYRQELKLDVSESAMAVVIQELAEGERSGVAFSKSPVEEDVSLVEAVWGLNQGLVD
                     GTVAPDRWVLDRSSGTLLRHIEAERSEAVRLAPEGTRKEPLLPELRNRSPLEDGEVGR
                     VWSLSMEAEKRFGCPQDVEWTFLGGRPFLLQARPVTAGAKGEPGSRAWNLSLRRSFEN
                     LKVLRERIEKRLIPALVKEARDLSGVDLDSLEDEELAKVLEIREEALARWTKVYWDEF
                     IPFAHGARLFGQVYNDRMQPEDPFRFIDLLRPGRMIGTDRNVRLEEAAERLRQDPALR
                     SSMTAFRDGRDEGDAAVFLEKVSSELGKDTDLALSGEETRRRLAGFLLEMAARPSMPK
                     RSGEAREDLEKAFLDRFQGMERNFAEELLDLARASYRLRDEDNIHLGRLERLRNEARD
                     ERLRRSGSVPSAGEPARAGANPRPVRPRQMVGSPAGPGLARGRARVVRDRRDLLEFKA
                     GEILVCDSIDPNMTFVVPLCAAIVERRGGMLVHGAIIAREYGLPCVTGVPDATERIRS
                     GDTLTVDGYLGIVVVHDPE"
     gene            complement(7651..8115)
                     /locus_tag="PLY83_03805"
     CDS             complement(7651..8115)
                     /locus_tag="PLY83_03805"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOV29196.1"
                     /translation="MRRVKENDQEVARAVGALLESQRVGVLGTFGAGSPYCSLVGFSF
                     EPGYGRLYFATTRATRKFANLSETGRVALLVDNRGNREEDLHEASAVTAVGPVTELTG
                     TEKDRGAAVHLTRHPSLAGFLESPTTALLALDVERYFLVRRFQEVMVLEVKR"
     gene            8269..8925
                     /locus_tag="PLY83_03810"
     CDS             8269..8925
                     /locus_tag="PLY83_03810"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOV29197.1"
                     /translation="MGYRMFRTIGALGLVLGFFFLSTAGAAPLPTVEVTEYQGIRLGS
                     VKDFRENSIRGVQKIDPARYRLVLDGLVEKPQSLSLEQVARMPRSRKVVRIDCVEGWS
                     VTALWEGVLVSDLLDLAKARPSAKIAIFHAVDGYTTSLPLDYLVSKKILLADTINGIV
                     LPPANGYPFQLVAEAKWGYKWIRWVNRIELSDNTAYRGTWEKQGYSNNGDASGPMFGD
                     "
     gene            8950..9267
                     /locus_tag="PLY83_03815"
     CDS             8950..9267
                     /locus_tag="PLY83_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29198.1"
                     /translation="MNEKTLYRANRFFAWLLLPVLAVNFVTGYAAVHPRLFGALVPKP
                     LAFRAHMAIQPLTVALVLFHGLYHVRLALLRRGLRGAFVDGSLAVLWLAGTAAAAWLA
                     RLG"
     gene            9324..9806
                     /locus_tag="PLY83_03820"
     CDS             9324..9806
                     /locus_tag="PLY83_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29199.1"
                     /translation="MAVTWYRQTQTGWKTIAVLAGLMTLMALAAFFLRRLGAPHEAQR
                     MGWGLVVVFLVVIATNRMEVRVTEREVFVRWGWLIGKHIPFSGIDRIEAERIPWTRVG
                     MKFTNGVSWFSVSPGPALRFHLKNGKSVVVGTADPGGLLRVLQPLKPELFKEKGEKNA
                     "
     gene            9803..>10374
                     /locus_tag="PLY83_03825"
     CDS             9803..>10374
                     /locus_tag="PLY83_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29200.1"
                     /translation="MKNPGAGIALVVFLVGVAGFTFFRAGRVEVVRPWPPRQKEADSL
                     SLPAPVAASLALASLAPSTHNTQPWKVRVISKDEVVVLLDETRLLPAVDPDRREAFVS
                     IGAFLENLVRGAGTQGLNAEIELKTEDPAKLEVARVKFEPAAERPDPKVLEAILSRNT
                     FRGDLPSTALPGETVQILEGTAPSRWTFFPS"
     assembly_gap    10375..10474
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10475..10803
                     /locus_tag="PLY83_03830"
     CDS             <10475..10803
                     /locus_tag="PLY83_03830"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOV29201.1"
                     /translation="FREAGIKKAQKQAGSCAGFFVLASPDGSPRSAVQAGRDLEAFWL
                     EATARGVAVQPMSQVLEESPWKDQIASRLGIGGTVQMVLRVGLAPTPPRAVSPRRSLG
                     SFVETP"
     gene            complement(10800..12050)
                     /locus_tag="PLY83_03835"
     CDS             complement(10800..12050)
                     /locus_tag="PLY83_03835"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOV29202.1"
                     /translation="MGRAVGEGFLSLWILGAVLVLAAVRKVGSVSLAIWQVMTAGALA
                     VLLTGQISPSEALHAVNADVMLFLFGMFVLGEALCASGLMSRFSMVLCGGAKSVGQLV
                     GILLFSMGLFSALLMNDTVAIIGTPLVLFLARRCSIPPKPLLLALAFSVTIGSVASPI
                     GNPQNLLIALASEGNPFVTFFRYLNLPTVICLAVAWLVLRFFHPGLFDRPLRIPSPEP
                     VKDPALARLCLVSIGLVLALIVLKVGLFFIRPGEDFPLSAIALAGCLPILLFSPRRGS
                     ILKELDWSTLAFFAAMFVLMAAAWESAPFQRLAIQAKSRLDSISEILAAGVLVSQVVS
                     NVPFVALSLPLLQGVGVKAAMALAAGSTLAGNLFILGAASNVIIIQNAEKRGETLTFW
                     EFARPGIPLTAACAGIYGVFLAVF"
     gene            complement(12103..13140)
                     /gene="mnmA"
                     /locus_tag="PLY83_03840"
     CDS             complement(12103..13140)
                     /gene="mnmA"
                     /locus_tag="PLY83_03840"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOV29203.1"
                     /translation="MPHLKPKKIAVLMSGGVDSTVAALLLKREGYDLAGLTLVLSGEA
                     PAEEAARQCEALGIPHAVLNLSGAFEERVVGPFRESYRRGETPSPCVDCNAAIKFGLA
                     WDFAEAALHAEAMATGHYARTGSDGKGPFLARGFDRGRDQSYFLYGIPLRKLERLLLP
                     LGSLTKTQVRAMAAEAGLSVCEKPDSMDLCFAGQGDYRALLEEGDFQPGPIVGLDGRL
                     LGEHRGLAGYTVGQRKGLGVAFPEPLYVVRIDRGTNTLVVAPREALLSCRVTAVRVNV
                     LSPGALEEEERLFGKIRSSGEPVPCRVARWDGVRLAVDFEEPVFAPAPGQKLVLYDEA
                     EHVVAGGTIQG"
     gene            13243..>14129
                     /gene="rarD"
                     /locus_tag="PLY83_03845"
     CDS             13243..>14129
                     /gene="rarD"
                     /locus_tag="PLY83_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HOV29204.1"
                     /translation="MELEISPAERRKGLLSMLAAYLAWGVLPVYWKALEEVPALTILC
                     HRIVWSLLFVGLLLAATRRTTGLRGVLKNRRILLLLGCSSLMIGINWLIYIWAVNAGK
                     ILETALGYYINPLVNVFFGVAFLKDRLRPVKWIAIGLAAAGVSFQVALLGHLPATALA
                     LAFSFALYGLIRKLTPVDALTGLFVETLILFLPALAWLALGPSAGFASGAPSRTALLA
                     GTGLVTSVPLIWFAYGARRLSLVTVGLMQYLSPTIGFFIGMFRYGERLTNVEWGTFAC
                     VWAALALYTASAFLESRGAA"
BASE COUNT         2539 a         4653 c         4262 g         2475 t
ORIGIN      
        1 gcgggatggc ctcggggatc ctggcccggg ccaggtcttc cacgacaccc accaggaaag
       61 gaatctccgc caccaggggc caccccgctt cgcgacaggc ttgccgcacg gcccgtgccc
      121 cttccgcgga aaggtccgac cggttcagga cgacggccat gggcacccgg aagcgcttcg
      181 ccagttcccc caggcgcttc aggtcgtgaa gcccgctttt ggacggttcc gtcaccgcca
      241 gggcgattga gcagcccgtc atggccgaaa tggccgggca ggcgatcccg gggggcccgt
      301 ccaccaggat ccaggacttt ccctgttcca gggccgatgc cctggctttc tcccgaagga
      361 gctgcacgag gcgtcccgag ttctccccgc cgggaaccag gcgcgcgtgc cagaaggggc
      421 cgaaatccgt ctcgctgccg taaatccgcc cctggaccgc cggggaaagg gaaaaagctc
      481 ccctcgggca caaaagagtg caggccccgc acccttcgca ccggctccgg tccaggatca
      541 cccttccgtt ttcggaagaa agggcgccga agcggcaaac gggaacacaa agcccgcaat
      601 cgtcgcaggg cggacggagg agggcggcct tgttcccgcc gacgaactcc tcctctgaat
      661 ggacactggg tttcaacagg atccacaggt ccgggacgtc cacgtcggca tcgcaaagaa
      721 cggcgtttcc cgaaagagcc ctggcgagca cggacgtcag ggtggtcttg cccgttccgc
      781 cctttccgct gacgaccacg atttcccgca ggctcatttg gcaaccctcc tcgcccattc
      841 ccaggcggcc cctgccgctt ccttccagga ggaatccact tccgtcgggg gaattccccg
      901 ggcagcgcct tccgccaccg cccgtgagaa aggaagcctt gcaagaaccg gcaacgttcc
      961 caggaccccc tcgaaagggg ggtcttcttc caggatcccc gaccggttca ccacgagagc
     1021 ggccggtttg cccatttccc ggaggacctg ggcgaccacc tcggcatccg cccttccgaa
     1081 aggggtcggc tccgtcacga ggatgaccgc ccttgctttc ctcacggccg ccaccatcgg
     1141 gcaggccgcg ccgggcggcg agtcgatcac cgtatccagc ccttccgcca ggcccagctc
     1201 cacggtacgc tcgatcacgg ggaccgggtt gggcatgccc accagcagcc gcccctccac
     1261 cacgacaatg ccgtttgcgt ccccccgccc gacgaatcca accggccgct caacctcgtg
     1321 gagggctttt tctggacagg ccaggacgca ggctccgcag ccgtggcaaa gatcgttgaa
     1381 aacgggaacg gccttcccga acgaaaaaac cgccccgaac cggcaagtcc gcgagcaggt
     1441 cccgcagcgg gtgcaccttt ccgcgtccac cgccgggacg ggcaggctca cgttcatggt
     1501 cgccaggacg gggtccccca ggagcagggc caggttcggt tcttccacgt cggcatcgac
     1561 cgcagcgaca ggttgcccgg acaaggcgag ggaggccgag aaagaggtct tccccgtgcc
     1621 ccccttgccg ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnagcgcttc
     1741 ctccgccggc ccggagaccc cgacgcacac ctggaccccc tgcgcctgga aaagcgagat
     1801 cgcccgttcc cccattcccc cagcgaggat cgccttcacg cccagccccg caagccacgc
     1861 cggaagcttt ccgggttcat ggcccgggtt ctcgtgcatc tcttttttga cgacatgccc
     1921 ttcttcggtc accgccacca ggaaatgcgg cgcatgtccg aaatgagcgc agacgcggcc
     1981 gccttccagg ggaatcgcga tcttcatccg ccctgtacct cctgtcctac tcttcggcct
     2041 tcgggttttc ccgcaaccgg gccacttcct cttccagcgc ttccagccgg gcccacagcc
     2101 cggttcccga aaggggttct ccggaattcc attgccgcca ggccccgaaa ccgcgaccaa
     2161 ggcttcgtcc aaaagatctg cccccgccgc aggggccccg tccccatccc gtcagggggc
     2221 ccactccgcg cgggcctgtc cgatccattc caggcatgag aatcgcctcc tttttgagat
     2281 tgattctcat tataattcgg ctatcaaaaa gatcaatccc ccggtatact tgttgggaag
     2341 gattttcaag aggaggggcg tgatcgacag gtggaaaggg aactcgtgaa ggtcggcatt
     2401 ttcatctgca accggtacca cacgtgcgcg gggggaaaat gcctccgggc cctgaaaaac
     2461 cgggaagggg ccttttctct ctacgagggg aaagacgtgg aactggtggg ctacacgacc
     2521 tgcgacggct gtcccggcgg gaatatcgag tacgccccgg ccgaaatgaa gaaaaacggg
     2581 gcgacggtga tccacctcgc caccgggatg ctcgtcggat accctccctg tccgcgaatc
     2641 gatttcttcc ggcggttcat ccgggaaaat tacgatctgc ccgtcgtcgt gggaacccac
     2701 ccgatcccgg aaaagtactt ccggatccac gaagcgctgg gaacctggaa ttccccgctc
     2761 tggcaggaac tgatcgcccc caccctggcc accccggaaa tccggctggc ctacaactga
     2821 aaaaaagtcc ccggctcgag ccggggactt tttttcgctt ttactccgtg actgtggctt
     2881 ccaccgcgca aaaggcttcg accaggggat cgagaagccc tggattgtcg tattcctcca
     2941 ggcgcccctt gtcccccagg cgcgagatga aggcatccat ggggaggctg gccagcttcg
     3001 ccacggacca tttcgtcgcc agcttgtcca ggtgactcgg accgaacagg tcccaccgct
     3061 cgccgcaatg gggacagagc gcgtaggcca tgttttcgac caggcccagg atcgggacgt
     3121 tcagcatccg ggccatgttc atggcttttt ccaccaccat gccggccagt tcctggggag
     3181 aggtcacgac gaccatcccg tccaggggaa ggctctgcat gacggtcagc ggggcatcgg
     3241 aggtccccgg gggcaggtcg acgatcaggg tgtccaggtc tccccagaag acctcctcgt
     3301 agaactgctt cacggtgttg gcaatgatcg gccctctcca gaccacgggt ttcgtcacgt
     3361 cgtcgagcag gaggttgatc gacatgacgg aaatgcccag gacgggagac tggggcggaa
     3421 cgattcccag gggcgatcct tccggaaggg cgcgcacgcc cagaagtttc gggatggacg
     3481 ggccggtgat gtcagcgtcc agcacgccga ccttcaaccc cttcctcgcc aggccgatcg
     3541 cgaggagcgc ggaaacggag ctcttcccca cgccgccctt tccgcttccc accgccacga
     3601 tccggcggat tcgccccttg tcgcttttgg ggggcagccc gcaggtttcg ggagtcgaat
     3661 tgcaggttcc tttctgggag cagctttcgc aggcattctc agacacgttg gttttcccct
     3721 ttcaaattca aacacaatgg attcggatca atccaggacc ttggccttcc ccgcaaggaa
     3781 cagccggaca ccctcttccg ccgtttcacc ctcggaaaga tacgccttca ggttcgccgc
     3841 cgcaagggca gaaacggcat tggggcctag tctcggcgcc aggaccgctc cgacgtcctt
     3901 ctgcgcaaga aggctaaccg cagcggctgc cgccccgtgc tccccggcct tgctgctcac
     3961 cgcctcgagg aacgtcccgt ccgaccggaa gagcaaaaac cagggtgccc tcgcaaaacg
     4021 gtccgatggc cttgcctgcg ggcttggccc ttccgcggcg atagcaatgt tcatcatgga
     4081 ttccccctgt caatatagaa actcattctc aataacaggg gtattgtaga acaccgcggc
     4141 agggagtcaa gagtgttcga acgggcctga aaaaggccct atcggttcca gcccaggcgt
     4201 ttcctgccgc agcaggtttc cgcccaggcg gctttgtcca tcggagctcc cttttccagg
     4261 caatcgggac agatcccgta aaactggaag gtctggggaa cgatccgaaa accggcctcg
     4321 ccgatccagg ccgccacgat ctcctgtttc gccgcttcgt cttccaggtg gaccatcttc
     4381 ccgcaggacc ggcaaagaac gtggaaatgc atcgtttcct gcgggatgtc gaaccgggcg
     4441 aacccctccc cctggtcaaa ggaatgaacc agtcccgttt cgccgaggac cagcagggtc
     4501 cggtagacgg tggcaaaacc gacggtcgga tccttggact gcacgcgctc catgagttcc
     4561 cgggcgttca ggtgctggcc ctggctttct agaagcgtct cgatgatcag gcgtctctgc
     4621 tccgtgcggc ggcacccttt ccggcggagg gcttccaggt actcttccgc cctctggcgc
     4681 aggtcttttc gatcggtttc agtcacgggc ctcaccctcc agcacgacct ccatgccggg
     4741 gacgacggtt cctccccgct cgacggaaag gaaaaacccc ttcgtcggca ggaggcacca
     4801 gccccggtag tcgtaagtat gaggctcgcc ggccttcttg cctttctcga caacgcggag
     4861 ccgaacgtcc ggcccgatgg cgagcccctg tcccgggagc aggtcttcgg gaagcccctc
     4921 gatcacgagg ttctcggcaa ggctcccggg gggaaagggg aagccggccg atctctccgc
     4981 ctcgcagatg tcttccgagc ggagcaggct gacctgccgt tcggtgagac cgaagtggga
     5041 gtccccctcg atgccctttc cgcgaaccag catcgcctcc gcctgcggct ctttcggttt
     5101 tctcctgtcc ttgctggtac agaccgccac gaccttagcc attccatttc ctctccttta
     5161 ccgttcttaa cggaacgatc cgtaaatcca ccgattcctt ttgggagaac gtagctaaaa
     5221 tgattcttgg ttcattatag gaggtgagaa gaatgcgtaa aggatggctc ctcttcatcc
     5281 tggcactggc cctggccgct ggagccctgg cgacgacggc tgcaggcccg gctctggcag
     5341 cggacaagct gcttgtctgc ggcatctgcg acaagtcttt ctacgaaacg gccgtgaagg
     5401 tggtccagga gatgggcctg gccgaccggg taaccgtgaa gaaaagctcc tgcctgggcg
     5461 cttgttccca acccccggtg gtcgagttca aggggacgct gtacacttcc atgacggcag
     5521 aaaaactgaa ggccctgctg gcgaaaacct actccgggtc gtgaaccacg acgatgccga
     5581 ggtagccgtc aacggtgagc gtgtccccgc ttcggatcct ttcggttgcg tccggaacgc
     5641 ccgtaacaca ggggagaccg tattcccgag cgatgatggc cccgtgaacc agcatgccgc
     5701 cccggcgctc gacgatcgcc gcgcagaggg gaaccacgaa ggtcatgttg gggtcgatgg
     5761 agtcgcagac caggatttct cccgctttga actcgaggag gtctctgcgg tctcgcacga
     5821 ctcgggccct tccccgggcc aggccaggcc cggccggaga gcccaccatc tggcggggcc
     5881 tcacggggcg cggatttgct cctgcgcggg ccggctcccc ggcggagggg acactgccgc
     5941 tccggcgcaa ccgttcgtcc cgggcctcgt tgcggaggcg ttccagcctc ccgaggtgga
     6001 tgttgtcctc gtccctcaaa cggtagctgg ctcgggccag atccagcaac tcctcggcaa
     6061 agttcctctc cattccctgg aagcggtcta aaaaggcctt ttccaggtcc tccctcgctt
     6121 ccccggaacg cttcggcatg gaaggccttg ctgccatctc gaggaggaag ccagccaggc
     6181 gccttcgagt ttcctccccg gataacgcca ggtccgtgtc tttccccagc tcggaggaaa
     6241 ccttttccag gaaaaccgcg gcgtcccctt cgtcccggcc gtcccgaaag gcggtcatgg
     6301 aagagcgaag agccggatcc tggcggagcc gttccgctgc ctcttccagg cggacgttgc
     6361 gatcggtccc gatcattcgc cccggacgga gcagatcgat gaaccggaac ggatcctccg
     6421 gctgcatgcg atcgttgtac acctgtccga aaagcctggc cccgtgggca aaaggaatga
     6481 actcgtccca gtagaccttg gtccagcgag cgagagcctc ctcccggatt tccaggacct
     6541 tcgccagctc ctcgtcctcg agggagtcca ggtccacccc cgacaggtcc cgggcctcct
     6601 tcaccagggc cgggatgagt ctcttctcga tccgttcccg aaggaccttc aggttttcga
     6661 agctccgccg aagcgagagg ttccaggccc ggcttcccgg ctctcccttt gctccggcgg
     6721 tgacgggcct tgcctggagg aggaacggcc ggccccccaa gaaggtccat tccacgtcct
     6781 gagggcaacc gaagcgcttc tcggcctcca tggagaggct ccagaccctg cccacttccc
     6841 cgtcttcaag aggcgaccgg tttctgagct ccggcagcag gggttctttc cgggtccctt
     6901 ccggggcaag ccgcaccgct tcgctccgct ctgcctcgat gtgcctgaga agggtcccgc
     6961 tggaacggtc caggacccac cggtccggcg ccaccgtacc gtccacgagc ccctggttca
     7021 acccccagac ggcctccacg agggaaacgt cctcctcgac cgggcttttc gaaaaggcca
     7081 ctccggagcg ctccccctcg gccagttcct ggatgacgac ggccatggcg ctttcagaca
     7141 cgtccagctt cagctcctgc cggtacagca gggctccgtc ggaccagagg gaggcccaga
     7201 ccaggcggat gcgctccagg atcgcagagg ccccccggac attcaggaag gattcgtgca
     7261 gccccgcgaa ggaagccccg gcagaatcct ctcccggggc ggaagaacgg accgctacag
     7321 gccggttgcc gaaaagctct tcgatctcgg ccaaaagggc cgcacggagc gattcgggca
     7381 gcggggtctt caaaaagaag ttgcggatcc tcagggccgc gtcccacatc tcctcccacc
     7441 gcatggcttc gaagggcttc cggttcagct ccagcaggat ccgctcccga agccccgtgg
     7501 gcccgacata ggcccggtag gcctccaccg gcacgacggc cccccgcggc acggggaatc
     7561 cctccctccc caaccgggag agagcccacg ctttcccgcc cgcccggggg cggtccttct
     7621 ccgacagccg gtcaaagggg atcagaaggt tcatcgcttc acctccagga ccatgacctc
     7681 ctggaagcgc cggacgagga aatagcgctc cacgtcaagg gccagcaagg ccgtggtggg
     7741 cgattccaaa aaaccggcaa gggaaggatg gcgggtgagg tgaaccgccg ctccgcggtc
     7801 tttttccgtt ccggtcagct ccgtcaccgg tcccaccgcc gtcaccgcgc tggcctcgtg
     7861 gaggtcctcc tcccggtttc cccggttgtc caccagcagg gccactcgcc ccgtttcgct
     7921 caggttggcg aactttcggg tggcccgagt ggtggcgaaa tacaaccgcc cgtatccggg
     7981 ttcgaagctg aaccccacga ggctgcagta gggcgatccc gcgccgaaag tcccaagaac
     8041 gccgaccctt tgggattcca gcagggcccc gaccgcccgc gcgacttcct ggtcgttctc
     8101 cttcacccgc ctcaccgtcc tttcgtgttt cctttccgtt ctcaataagt gtaccccaga
     8161 aagcgtgggc ccaaacctgc tccaggcacc gctttagatt gacacagaaa cagagagaag
     8221 gaataatgaa gaaactcccg gaacgttttc aaaaaaggag ggaatttcat ggggtaccgc
     8281 atgtttcgaa cgattggggc gttgggcctg gtgctggggt tctttttcct ttcaaccgcg
     8341 ggggcagctc ccctgcccac ggtggaagtg acggaatacc aggggatccg gctcggatcc
     8401 gtgaaggact tccgggagaa ttccatccgg ggcgtccaga aaatcgaccc cgcacgctac
     8461 cgccttgtct tggacggcct ggtagagaag ccgcagtcgc tgtcactgga gcaggttgcc
     8521 cggatgcccc gctccaggaa ggtcgtccgg atcgactgcg tggaagggtg gagcgtaacg
     8581 gccctttggg aaggcgtcct cgtgtcggac ctccttgacc tggcgaaggc caggccttcc
     8641 gcgaagatcg ccattttcca tgccgtcgac ggatacacga cttctctccc ccttgactat
     8701 ttggtctcga agaaaatcct gctcgccgac accatcaacg ggatcgtcct gccacccgcc
     8761 aacggttacc ccttccagct cgtggcggag gccaagtggg gctacaagtg gatccgctgg
     8821 gtgaaccgca tcgagctgtc ggacaacacc gcctacaggg gaacctggga aaagcagggg
     8881 tatagcaaca acggagacgc ctccggtccc atgttcgggg attagcggaa aacctgggga
     8941 gaatcccgga tgaacgaaaa aaccctttac cgggccaacc ggtttttcgc ctggctgctc
     9001 ttgcctgttt tggcggtgaa cttcgtcacg gggtacgcgg cggtccaccc gcggcttttc
     9061 ggcgcgctgg tcccgaagcc cctggcgttc agggcgcaca tggcgatcca gcccctgacg
     9121 gtggcgctgg tccttttcca tggcctttac cacgtgcggc tcgccctcct ccggcgggga
     9181 ctgcgagggg cattcgtgga cggttccctc gccgtcctgt ggctcgcggg aaccgccgct
     9241 gccgcctggc ttgcccggct aggctaacgg gcttcgaggt gacacaatag gaggcgatcc
     9301 tgctgaaacg gagggaaacg gaaatggcgg tgacctggta ccgacagacg cagacgggat
     9361 ggaaaacgat cgccgtcctt gcgggcctca tgacgctcat ggctcttgcc gccttcttcc
     9421 tcaggcggct gggggccccc cacgaggcgc agcgcatggg ttgggggctc gtggttgtct
     9481 tcctggtggt gatcgcgacg aaccggatgg aagtgagagt caccgagagg gaggtcttcg
     9541 tccggtgggg atggctgatc ggcaagcata tccccttttc cgggatcgac cggatcgaag
     9601 ccgaaaggat cccctggacc cgtgtcggga tgaagttcac gaacggtgtt tcgtggtttt
     9661 ccgtttctcc ggggccggcc ctccgctttc acctgaaaaa cggcaaatcg gtcgtggttg
     9721 gaacggcgga cccgggcggc ctccttcggg tccttcagcc cctgaagccc gagctgttca
     9781 aagagaaggg agaaaagaac gcgtgaagaa cccgggagcc ggaatcgccc ttgttgtttt
     9841 cctcgttgga gtggcagggt tcactttttt tcgggcaggc cgggttgagg ttgttcgccc
     9901 ctggccgccc cggcaaaagg aagcagacag cctttccctt ccagcgcccg tggcggcttc
     9961 cctcgcgctg gccagcctgg ctccgagcac ccacaacact cagccctgga aggtccgggt
    10021 gatttcgaaa gacgaagtcg tcgtcctgct ggacgaaacc cgcctgctgc ccgccgtgga
    10081 tcctgaccgg agggaagcct tcgtctcgat cggggccttc ctggaaaacc tcgtccgcgg
    10141 ggcgggcacc caggggctga acgcggagat cgagctcaag accgaagacc cggcaaagct
    10201 cgaggttgca agggtgaagt tcgaaccggc cgccgaaagg ccagacccga aggttctcga
    10261 ggccatcctt tcgcggaaca cgtttcgggg agatctcccc tccaccgcgc tgcccggtga
    10321 aacggtgcaa atcctggaag ggacggcccc ttcccgctgg accttcttcc cgtcnnnnnn
    10381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgttcc gggaagccgg gatcaagaaa
    10501 gcccaaaagc aggccggctc ctgcgcgggg ttcttcgtcc tggcaagccc ggatggaagc
    10561 ccccgttccg ccgtccaggc cggccgggac ctggaggcct tctggctgga agccaccgcc
    10621 aggggggtcg cggtccagcc catgagccag gtgctggaag aatccccgtg gaaggatcaa
    10681 atcgcttcca ggctcgggat cggggggacg gtgcagatgg tcctcagagt cgggctggcg
    10741 cctacgcctc ccagggcggt aagcccgcgg cgttcccttg gaagcttcgt cgagaccccc
    10801 tagaaaaccg ccaggaatac cccgtagatg ccggcacagg ccgcggtgag gggaatgccg
    10861 gggcgcgcga attcccagaa agtcagggtt tcgccgcgtt tctcggcatt ctggatgatg
    10921 atgacgttgc tcgccgcacc caggatgaag aggtttcccg ccagggtgct tcccgcggcc
    10981 agtgccatgg cggctttcac gccgaccccc tggaggaggg ggagggaaag ggccacgaac
    11041 ggcacgttcg acacgacctg gctcaccagg acgccggcgg caaggatttc cgagatcgaa
    11101 tcgaggcggc ttttggcctg aattgccagc ctctggaaag gggcgctctc ccaggctgct
    11161 gccatgagga cgaacatcgc ggcgaaaaac gccagggtgg accagtccag ttccttgagg
    11221 atcgagcccc ggcgcgggct gaacagcagg atcggaaggc atcccgccag ggcgatggcc
    11281 gaaagcggga aatcttcgcc gggacggatg aagaacagcc cgaccttgag gacgatcagg
    11341 gccagcacaa gcccgatcga caccaggcag agacgggcca gggctggatc tttcaccggt
    11401 tcgggggaag ggatgcggag gggtcggtcg aaaaggcccg ggtggaagaa gcggaggacc
    11461 agccaggcca ccgccaggca gatgactgtc ggcaggttca ggtagcggaa aaaggtcacg
    11521 aaggggttcc cctcggaggc aagggcgatc aggaggttct ggggattgcc gatgggactc
    11581 gccacgcttc cgatcgtcac ggaaaaggcg agggccagaa gcaggggttt cggcgggatc
    11641 gagcagcgcc gggccaggaa gaggacaagg ggcgtcccaa tgatcgccac cgtgtcgttc
    11701 atcaggaggg cggagaaaag ccccatcgag aacagaagga tgccgaccag ctgccccacg
    11761 cttttcgccc cgccgcacag gaccatggaa aaccgcgaca tgaggccgct ggcgcagagg
    11821 gcctcgccca gcacgaacat tccgaacagg aagagcatga cgtcggcgtt tacggcgtga
    11881 agggcctccg acggggaaat ctgccccgtc agcaggaccg ccagggcacc ggcagtcatg
    11941 acctgccaga tcgcaagact gacagagccg accttcctga ccgcggccag gacgagcacg
    12001 gccccgagaa tccaaaggga caggaagcct tcccccactg ctctacccat ctcctctccc
    12061 ggatgaccag cgcgaaatcc tagcgcaagc ccgccctttt ggtcaaccct ggatcgttcc
    12121 cccggccacc acgtgttccg cctcgtcgta gaggaccagc ttctggcccg gcgccggggc
    12181 aaagaccggt tcctcgaaat cgaccgccag ccggaccccg tcccagcggg ccactcggca
    12241 cggtaccggt tccccgctcg atcggatctt tccgaaaagg cgttcttcct cctccagggc
    12301 cccgggggac aggacgttca cgcgaacggc cgtgacccgg caagaaagga gggcctcccg
    12361 cggggcgacg acgagcgtgt tcgtcccccg gtcgatccgc accacgtaga gcggctccgg
    12421 aaaagccacc ccgagtccct ttctctggcc gaccgtgtac cccgcaagcc cccggtgctc
    12481 ccccaggagc cggccgtcca ggccgacgat gggacccggc tgaaaatccc cctcttccaa
    12541 aagcgcccgg taatccccct gcccggcgaa acagaggtcc atgctgtccg gcttttcgca
    12601 gaccgaaagc ccggcctcgg ccgccatcgc ccgcacctgg gttttcgtca gggacccgag
    12661 cgggagcagg agccgctcca gcttccggag ggggatgccg tagaggaagt aactctggtc
    12721 cctgccgcgg tcaaagcccc gggccaggaa gggccccttc ccgtcggagc ccgtccgggc
    12781 gtagtggccg gtcgccattg cctccgcatg aagggccgcc tcggcaaaat cccaggccag
    12841 gccgaacttg atcgcggcgt tgcaatccac gcagggactg ggtgtttcgc cccgccggta
    12901 gctttcccgg aagggcccca cgacgcgttc ttcgaaagcg ccggataggt tgagtacggc
    12961 atggggaatc cctagggcct cgcactgccg cgccgcctcc tccgccgggg cctccccgga
    13021 aaggacgagg gtcagccccg ccaggtcata cccttctctt ttcaggagaa gagccgccac
    13081 cgtgctgtcc acgcccccgc tcatgaggac cgctattttc ttgggtttca ggtgcggcat
    13141 gaagacctcc aggaaaattc cctcttcgtt ttcggtcaaa cccgactcta ttctgtacaa
    13201 tatacacgat ccgacgaaaa ggaattttcg aggaagggac cggtggaatt ggaaatttct
    13261 cctgccgagc gccgaaaagg gcttctctca atgctggcgg cctacctggc ctggggagtt
    13321 ctccccgttt actggaaagc cctggaggag gtgccggcgc tgacgatcct ctgccaccgc
    13381 atcgtctggt ccctgctctt cgtcggcctg cttttggccg cgacccggcg cacgacggga
    13441 ctgagagggg ttttgaaaaa ccggcggatc ctcctcctgc tgggctgcag cagcctgatg
    13501 atcggcatca actggctgat ctacatctgg gccgtcaacg cggggaagat cctggaaacg
    13561 gccctggggt actacatcaa cccgctggtg aacgtcttct tcggggtcgc ctttctcaag
    13621 gaccggcttc gcccggtcaa gtggatcgcg atcggcctgg cggccgcggg agtctccttc
    13681 caggttgccc tcctgggaca cctcccggca accgcgctgg cactggcctt ttcctttgcc
    13741 ctgtacggcc tgattcgaaa gctcaccccc gtggatgccc tgaccggtct tttcgtggaa
    13801 accctgatcc tcttccttcc cgccctggcc tggctggccc tcggcccctc ggcggggttc
    13861 gcttcgggag ccccttcgcg aacggccctt ctcgcgggaa ccgggctggt cacctcggtt
    13921 cccctgatct ggttcgctta cggggcacga aggctgagcc tggtgacggt cggactgatg
    13981 cagtaccttt ctcccaccat cgggtttttc atcgggatgt tccggtacgg ggaacggctg
    14041 acgaacgtcg aatgggggac atttgcctgt gtctgggccg cgctggccct ctacacggca
    14101 tcggccttcc ttgagtcgcg tggcgccgc
//
LOCUS       DAOOVN010000026        13835 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_2962_length_13835_cov_12.020102, whole genome shotgun
            sequence.
ACCESSION   DAOOVN010000026 DAOOVN010000000
VERSION     DAOOVN010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 13835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13835
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2962_length_13835_cov_12.020102"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..844)
                     /locus_tag="PLY83_03850"
     CDS             complement(74..844)
                     /locus_tag="PLY83_03850"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOV29205.1"
                     /translation="MAKIPLVQSINRALDILELLSREEKLGITQIGQAVELDKTTVFR
                     IVQTLCVRGYIRQDPSNQMYSNTLKLFQMGAGIVDRRRILGKARPHLEALAISTRETV
                     NLAVIDGGNIVYVDKIESTEVIKADLGVGKSYPAYATSLGKSILALLGENELERLYRG
                     DVFSAFTQHTVKNLDELKKQLKKVRAKGYASDDEELVTGLSCVAAAIRDFQGKPVAAV
                     SIAFPSYRVLADSPERSRMAELVQMTARSISIELGFRE"
     gene            969..1934
                     /locus_tag="PLY83_03855"
     CDS             969..1934
                     /locus_tag="PLY83_03855"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HOV29206.1"
                     /translation="MIANYPTFVAMKKIFHLYINGGYFFMTSVILEGNALERISTVAK
                     NLSPKQHKLARYIFENSLQASFMNITTLSGAAGVSESTVVRLANALGYPGFPELQHSL
                     REIAQGQISSLDKYSLDGEGNNLPLFKKVFNLEMTLLRDTMENIPEENFESAIQKLRE
                     ASHVFVVGAGPNQSVAVYFSFFLQVLRPGVSMISNLDLGLSHLLKSHSKGSLAVIFSF
                     PRYPAVTQKIAEKFNERHIPVIGITDSSLSPLAPLCDFLFESKMRFISFIDPSAATMA
                     LMHSLLIGLFLKDPAYAKKQLRHFEDQIQQDKYFLRQDLDIVDLL"
     gene            2044..3063
                     /gene="dctP"
                     /locus_tag="PLY83_03860"
     CDS             2044..3063
                     /gene="dctP"
                     /locus_tag="PLY83_03860"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOV29207.1"
                     /translation="MKVRFRLFSLVTILLFGLAMVASFSSTASAAEKLVLRMAGQSPI
                     EYQGTIHQMEFKKTIEAATKGRIQIKVYPANQLGDYTQVYEEIMKGTIDMALISVPSQ
                     FDQRLELTYVPYLAENYDQVKKFFAQNGFLFNTLSKVHDKLNVKLLGFNIEGFGGLGS
                     TKPLVEPANPAVKKGILLRVPPMDVFKFWALALNFQTVTVPYAELYTALQTGVAEGWI
                     GGAPVLTYLSFRDVIKYYYQYNGHVESESWLINKDLWNKLDKADQKLFLDTVAKLQAK
                     SIAVSEKEDKEYLKKLADESKIKVTTFSNAELKKFAETVRKTAWPKLKDRLTPEIMDA
                     LMKQY"
     gene            3153..3707
                     /locus_tag="PLY83_03865"
     CDS             3153..3707
                     /locus_tag="PLY83_03865"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOV29208.1"
                     /translation="MAAECQETLAKGYLEESHGTVAKALLKVLRFIMISMNLTVAVVI
                     GVAAALRYVFKMDMYGIEDFIIVFAYWLYFSGGAYGAFENSHITADILTVYMKNKKLK
                     RGIICFSSLCTVLLSGLFAYWGWLMFSWQLAKGGASPVWRIPLYIPQSAITLGLTLMT
                     FYFALHFIENLRLFRKALSGKAEV"
     gene            3713..5011
                     /locus_tag="PLY83_03870"
     CDS             3713..5011
                     /locus_tag="PLY83_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOV29209.1"
                     /translation="MIYVALLILLAALVVGIPVPFSFLASSLFLVVTGGYSPSFLLPY
                     GYSKMSTVVLLAIPLFITAGGLMERGNIGQQLIDMVDLFVGRLKGGLGAVAVVSCAVF
                     GSITGSCCATLSCIGAIMFPRLEKAGYPLGHSAALMANSAVLGILIPPSAIMILYSWV
                     GGQSVLACFLSTVIPGIILTVLFAVCNVFMVRKNPNLVLSQELAPGELGRLFVKRGSR
                     ALPALFMPVMILGGIYGGMFTPTEAAALSVLYAIPVGMFVYGGLNKDSLKTVLLESAT
                     TTGVVMVMLYAVMILSRLYIMEDMPQKLLELLTSVTKSKIGILLILNLFMVIMGMLMD
                     DVSAVMLCTPILLPIAQAIGVHPIHFAAILGVNLGMGNITPPCAPVLYLSGRLNGAAI
                     SDTLMPTMTLIAFGWVPVLLLTTYVPELSLWLPRLILGIR"
     gene            5044..5436
                     /locus_tag="PLY83_03875"
     CDS             5044..5436
                     /locus_tag="PLY83_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HOV29210.1"
                     /translation="MNRVVTTDKAPAAVGAYSQGVYAGPFLFLSGQIALDPQTGKMVG
                     GGDPAAETVQVMKNIQGVLESQGMTMDNIVKAVIYAASIKDFKAINDVYKSFFPNGYF
                     PARCFVEVGGMALDAKVEIDVIATTEQS"
     gene            5457..6752
                     /locus_tag="PLY83_03880"
     CDS             5457..6752
                     /locus_tag="PLY83_03880"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF015284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOV29211.1"
                     /translation="MGTQSAIDILRKHTFLTLGCTDPVAIGLAVAHACRISEGEIRGI
                     KVRLDRNIYKDAVSVGIPGTHSSGVHLAAALAAIKGHPESGLELFKDVEPTDIGLAMS
                     FLETHDIRFEIDEEAEGIRIAATVTTEKGVYEALIEGAHNRLVEKRANGKTVYRAEKA
                     CDESEDTDMNGLDQLRDLTMEQIFELLKGLPDRDLSFLLEGVARNLDAAKAGLADQAG
                     MELGRSLEKLLENSLLPDSPINRIRILAAAAADARMGGLKTPIFGCYGSGNHGITFFI
                     TVGEMGRYLGRSDAEIARALALGLLVIGVIKTRTGILTPHCGCAVAAGTGAAAAIVWL
                     LGGDAKQVESAVQLMTANLTGMLCDGAKYGCALKIATSVGIAIETAYLALGGVTVPSG
                     NGIVGRTFQQTMDNLETVTSKGMKDVDQAVLDVLLAGQN"
     gene            6772..7761
                     /locus_tag="PLY83_03885"
     CDS             6772..7761
                     /locus_tag="PLY83_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HOV29212.1"
                     /translation="MAYDVLWISQEEVVSLGITMKEIVDAVEEGWKLKGQKKVELPAK
                     IGVHPRKDCFIHAMPSWVGGDVDCCGIKWAAGYPSNQAKGLPYINGVWCMNDPETGII
                     KAVMDANWMTATRTGAASGVCAKYLADPKSEVVSIIGLGAQGRTNLLAFKETLPQMKT
                     VKVYDIFESQVTKFVNELGPQLPGVDFVVGRNVEEAVKDADVIATCAPIVEKPERFVR
                     SSWLKKDVLAIAVDYDSSFDADIMNNGNCFVCDDWNQYLYTQALNIYFQAGYPKKEEI
                     YADMGEICAGVKPPVLQGRRGAVLMGIASHDIMTGRLIYRKAIEKKVGTWVKL"
     gene            7835..9100
                     /locus_tag="PLY83_03890"
     CDS             7835..9100
                     /locus_tag="PLY83_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HOV29213.1"
                     /translation="MNLTEEERRMLDGQLGPLKRKALEFVVSYANVLGAESLCRVSKA
                     HLFCGAHHYLEAVRSTDMDEVISEMWFCSGETIPFDSVGCFAQSDCSPVDHLNWLKMG
                     CTEEEFLKNERYLDRCLRAGVHLVGSCVPYMTGFIPLRGEHYVTTESHAVLMMNSLWG
                     ACANADGIEAAFCSAVCARTPRWGMHVPESRKGTHLFYLTCEPETVEDWDLVGYAVGL
                     RLPPHSVPILAGNVENADMVRLKSCFAAMATTAGPELCHILGVTPEAPTLASCLSPSG
                     LEDEVEIGPKQLRQAREILCDSRPGRVDYISLGCPHYSIDQVGEVASWLKGKKVFPGT
                     TLHVWTALPIRAVADRCGYTRTIEEAGGTLMVGSCPLVSEKWPSGTTAMAFDSAKQAH
                     YIKPLTDAKVYFGSSGECLEAAVSGRWGR"
     gene            9106..9540
                     /locus_tag="PLY83_03895"
     CDS             9106..9540
                     /locus_tag="PLY83_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HOV29214.1"
                     /translation="METVVIRGRGAVPGIATGPAMVCRETIQGWSGIDEKTGKVIEKG
                     HPAEGQTIRGVVLILSGGKGSNGWSSHFHAARIRGISPCALVFPRIDSRTGVAAVVCG
                     VPTVVDLEEDPFKIIENGDQVTVDGNSGTLTIYKGPFPTKGA"
     gene            9545..9817
                     /locus_tag="PLY83_03900"
     CDS             9545..9817
                     /locus_tag="PLY83_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29215.1"
                     /translation="MNRSRTSVMERMYRSDMALVGTFFILMWTVLLYVLLQVNRIAPD
                     TTTRTLATVAALLVGGFASGALAAVAIHLRKHREDLYREDCKNSGL"
     gene            9838..10161
                     /locus_tag="PLY83_03905"
     CDS             9838..10161
                     /locus_tag="PLY83_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29216.1"
                     /translation="MPRQKGKNLFMEVFDVLFIMLLCFATLLSTMLMRGKVLVGSGSG
                     GGMAYTFSAWTFLLTFLGLVAYLAYVIPQSNRELGEIVECLYGEPRENLPPEVPLEPA
                     AEASK"
     gene            10281..11648
                     /locus_tag="PLY83_03910"
     CDS             10281..11648
                     /locus_tag="PLY83_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOV29217.1"
                     /translation="MAEEKPHLSKVLGPVHIWALGVGIVLVGEFMGWNFTVAKGGTLG
                     AIIACWVMGLLYTSLVMVNTEMGSVIPEAGGQYAMAKYFMGPLAAFNVGLMLVFEYVM
                     LEAADALVVGEILKTLSPHLDSLPFIILTLVGLTWLNYRGVYATLTFNFVITAIAYMT
                     IFVLLFSTKFFIPGSSLLKLAEFTDGLPYGWIGIVGAFQFGIWFYLGIEGTALAAEEC
                     RSTGRSLPVGALTGMVTLLIGATITWFVCSGLVHSKELGASVYPLFDAALATGMPAVI
                     ALLFAGTILSCMASANGCITDASRAWFAMARDTLIPEYFAAVHPKYRTPYRAILFQLP
                     IALAFGFTGLLDQVITFSILSALLVYLFTAVMMFKFRRMYPLGSIERGYLSPWHPWTT
                     LLLLVLTLATLLGMYFGYWLNMLAGFLFYSLASLWFALHRYRFVDREKLISEGASRWP
                     RPVGY"
     gene            11653..11904
                     /locus_tag="PLY83_03915"
     CDS             11653..11904
                     /locus_tag="PLY83_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29218.1"
                     /translation="MRLPRWAILLAAFLVPGSGHVLLGRPTRGLVMLFWMVIFGYVTF
                     RLAPPQTAFLGRIGGGLAVWALSVLDVAHINIMALKVTK"
     gene            11922..13031
                     /locus_tag="PLY83_03920"
     CDS             11922..13031
                     /locus_tag="PLY83_03920"
                     /inference="COORDINATES: protein motif:HMM:NF014381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HOV29219.1"
                     /translation="MGKVIAILGGGNAGQASAAELTLLGHECRLYQDPRFAEDLRLVL
                     ETGKIRLEADPKGGCRATGLAAISKASCDMAETVRGAEIILCHLPAFAHADVAEQLAD
                     LVEDGQVIVLLPGTLGTLVFVETFKRKGVAADVLIAETNTNSYDTRVLGPGQAYVYKM
                     NDPLQIGVFPSSRTAEALEKLDGVYAFVPVSDILEAAFHSHNPVIHTPACIMSVSRIE
                     RSRGDFYLYEEAFTQTVCRVTKMLDEERMAVARAFGYAPLTLEQLLSGLEQPGDLFKE
                     TNGNPGLCFIRGPESVKSRYFTEDGLNGLVPWSQLARTAGIPVPLMEAFATIEGAVIE
                     RDMWREGRTLKTLGLEGMNVTEIRKYLREGKKTGA"
     gene            complement(13252..>13835)
                     /locus_tag="PLY83_03925"
     CDS             complement(13252..>13835)
                     /locus_tag="PLY83_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29220.1"
                     /translation="GISSRYGIPVYVTAATEGALACRGLGAGLASVCRFSPGDCLEFG
                     SVRVETVPTPHDSVEGSVFVIEDGGLRLGILTDLGHVFDGLGRVVRSLDAVFLESNYD
                     SGMLKRGKYPAFLKKRITGPGGHLSNEESAALVADSGRVFSWVCLSHLSGENNTPDTA
                     VETHRQIVGAQQDIHVAPRDRVGELLVVSKADE"
BASE COUNT         2992 a         4146 c         3825 g         2872 t
ORIGIN      
        1 agactgcttc atctgcacca gttccagacg ccccttcttc ttcgccatga cgatccctcc
       61 gttttttaat ttctcattcc cgaaacccca actcgatcga aattgaacga gcagtcattt
      121 gaaccaactc cgccatcctc gatctttccg gggaatccgc tagcactctg taactcggga
      181 acgcaatgct taccgcagcc actggcttcc cctgaaaatc ccttatcgct gccgccacgc
      241 acgaaaggcc agtaacgagc tcctcgtcat ctgatgcata tcccttggcc cggacttttt
      301 ttaattgttt ttttagttca tctagatttt tgaccgtgtg ttgggtgaaa gcagaaaaaa
      361 catcccccct ataaagcctt tccaactcgt tttccccaag gagagctagg atcgactttc
      421 ccaggcttgt ggcataggct gggtaagatt ttccgactcc caggtccgcc tttatcacct
      481 cagtgctctc aatcttgtcc acatagacga tattgccccc gtctattaca gctaagttga
      541 ctgtctctcg tgtgctaatc gcaagcgctt ccaggtgcgg cctggctttt ccgaggatcc
      601 gtcgcctatc gacaatacct gcccccattt gaaaaagctt gagggtgttc gaatacatct
      661 ggttggaggg atcttgccga atgtatcctc tgacgcaaag agtctgaaca atcctgaaaa
      721 cagtcgtctt gtccagttcg actgcctgcc caatctgagt gatgcccagc ttttcctccc
      781 tgctcaaaag ctcaagaata tcgagcgccc tgttaattga ctgaaccagt ggaatcttag
      841 ccattgcacg gccccctgtt ccgaataacg aaacatacgt ttcgcaaact ctacttcatt
      901 attggccggc tagtcaacta cgtaccatta aagtaatttt tcactttacc gaattgtttg
      961 acattttctt gattgcaaac tatcctacgt ttgtggctat gaagaaaatt tttcatcttt
     1021 atatcaatgg ggggtatttt ttcatgacga gtgtgatcct agaaggaaac gctctcgaaa
     1081 ggatctcgac cgtcgcgaag aaccttagcc cgaagcagca caagcttgcc cgctatatct
     1141 tcgaaaacag cctgcaagcc agcttcatga acattaccac gctgtcggga gctgcgggag
     1201 tcagcgaatc gacggtcgtg aggttagcga acgcattggg gtatcccgga ttcccagagc
     1261 tccagcactc gctgagagaa atcgcccagg ggcaaatctc ttctctagac aagtactccc
     1321 ttgacggtga aggaaacaac ctgcctcttt tcaagaaggt attcaaccta gaaatgaccc
     1381 tgctacggga cacgatggaa aatattcctg aggaaaactt tgagagcgcc atccaaaaac
     1441 tccgggaagc ttcccatgtg tttgtcgttg gcgctggccc caaccagtct gtggcggtgt
     1501 atttctcctt cttcctgcaa gtcctgcgcc ccggagtttc gatgatttcc aacctggacc
     1561 tcgggctgtc ccacttgctg aaaagccata gcaaaggctc cctggccgta attttcagtt
     1621 ttccgcgcta cccggcagtc acgcagaaga tagccgagaa attcaacgaa cggcatatcc
     1681 ccgttatcgg cattaccgat agctccttat cccctcttgc acccctttgc gatttcctct
     1741 tcgagtccaa aatgagattc atctctttca tcgatccatc ggcggcaacc atggccctga
     1801 tgcattctct gctcatcggc ctgttcctga aagaccccgc ctacgcaaaa aaacagcttc
     1861 gccatttcga agaccagatc cagcaggaca agtacttcct gaggcaggac ctcgacattg
     1921 tagatttgct ttagaggggg gggagaaaac aaaggaatga caggcaaggt ccttggtttt
     1981 tctatcaggc acttgaaacg gaaagaaacg accatcttta tcgcggaaaa gggggaaata
     2041 gcaatgaagg taaggtttcg cctgttcagc ctggtaacaa tccttttgtt cggtctggca
     2101 atggtggcat ccttcagctc caccgcttcc gccgcagaaa agctcgtgct cagaatggcc
     2161 ggacagagcc ccatcgagta ccaaggcaca atccaccaga tggagttcaa gaaaaccatc
     2221 gaggcagcca cgaagggccg tatccagatc aaagtctacc ccgcaaacca gctgggcgac
     2281 tatacccagg tctacgagga aatcatgaaa ggcaccatcg acatggccct gatttccgtc
     2341 ccgagccagt tcgaccaacg ccttgaactg acctatgtcc cttacctcgc tgaaaattac
     2401 gaccaagtca agaaattctt tgcacaaaac ggattcctgt tcaacaccct aagcaaggtc
     2461 cacgacaagc ttaatgtcaa gctcctaggt ttcaatatcg agggatttgg cgggctaggt
     2521 agcacaaaac ctctcgttga acccgcgaac ccggcagtca agaagggcat cctgctccgc
     2581 gtcccgccga tggacgtctt caagttctgg gccctcgcgc tcaacttcca gaccgtcacc
     2641 gtcccatacg ccgagcttta caccgccctt cagaccggcg tggcggaagg ctggatcggc
     2701 ggcgcgcccg tcctgaccta cctgagcttc cgcgacgtca tcaagtacta ctatcagtac
     2761 aacggacacg tagaaagcga gtcgtggctc attaacaagg acctttggaa caaactcgac
     2821 aaggctgacc aaaagctctt cctcgacacg gtggcaaagc tccaggcgaa gagcatcgcg
     2881 gtctcagaga aggaagacaa ggagtacctc aaaaaactcg ccgacgaaag caagatcaag
     2941 gtgacgactt tctccaacgc agaactgaaa aaattcgcag aaacggtccg taagacagcg
     3001 tggcccaaat tgaaggatcg cctgaccccg gagatcatgg atgccttgat gaaacagtac
     3061 tagcgcctcg aaaccgggag gccgggaaaa aacaggcctc ccggcactct cctcgctttc
     3121 ttcaagatgg tgtctgaaag gtgggcaagg acatggcagc cgaatgccag gaaaccctcg
     3181 caaaaggtta cttggaagaa tcccacggaa ccgtcgcgaa agccctatta aaggtcctgc
     3241 gcttcatcat gatctcgatg aacctgaccg tggcggtcgt gataggagtt gccgccgccc
     3301 ttcgctacgt gttcaagatg gacatgtacg gcatagagga tttcatcatc gtcttcgcct
     3361 attggctgta tttctccggc ggtgcttacg gagccttcga aaacagccac atcaccgccg
     3421 acatcctgac agtctacatg aagaacaaga aactgaagag gggaattatc tgcttcagtt
     3481 cactctgcac ggttcttctt tccggtcttt tcgcctactg gggatggctc atgttttcct
     3541 ggcaactggc caaaggaggt gcctccccgg tctggaggat cccgctctac attccgcaaa
     3601 gcgccatcac cctaggccta accctgatga cattttattt cgcgcttcac ttcattgaaa
     3661 atcttcggct tttccgcaaa gctctgtccg gtaaagcgga ggtgtagcca ttatgatcta
     3721 cgttgccctg ctcattcttc tggcagctct cgtcgtcggg atcccagttc ccttctcgtt
     3781 cttagcttcc agccttttcc ttgtggtgac gggcggctac agtcccagct tccttcttcc
     3841 ttacggttat agcaagatga gcacagttgt cctgctggcc atccccctct tcattacagc
     3901 cggtgggctt atggagcgtg gcaacatagg ccagcagcta atcgacatgg tcgatctgtt
     3961 tgtaggtcgt ctcaagggag gtttgggagc tgtcgctgtc gtctcctgcg ccgtgttcgg
     4021 ctcgattact ggtagctgtt gcgccactct atcttgtatc ggggccatca tgttcccccg
     4081 cctcgaaaag gcaggttatc ccctcgggca ttctgcggcc ctcatggcga acagcgccgt
     4141 attggggatt ctcatcccgc ccagcgccat catgatcctg tattcctggg tcggaggcca
     4201 gtcggtgcta gcctgtttcc tttccaccgt cattcccgga atcattctga ccgtgctttt
     4261 cgccgtctgc aacgtcttca tggtaaggaa gaaccccaat ctggtccttt cccaggagct
     4321 tgccccaggg gaactcggca ggctgtttgt caagaggggt tcaagagccc tgccagcgct
     4381 cttcatgccc gtcatgatcc tcggaggcat ctacggcggg atgttcacac cgaccgaagc
     4441 agcggctctc tcagtgctct acgccatccc tgtagggatg tttgtctacg gaggcctgaa
     4501 caaggattcc ctgaaaaccg tcctcctcga atcagccacc acgacagggg tggtcatggt
     4561 catgctttac gcagtcatga ttctcagccg cctctacatc atggaagaca tgccccaaaa
     4621 gctgctcgaa ctgttgacct ccgtcacgaa gagcaagatc gggatcttgt taatcctcaa
     4681 cctcttcatg gtcatcatgg gcatgctcat ggacgatgtg agcgccgtca tgctctgtac
     4741 gccgatcctg ctcccgatcg cccaggccat cggggtccac ccgatccact tcgcggcaat
     4801 ccttggcgtg aacctaggaa tgggcaacat cacgcccccc tgcgccccag tcctgtacct
     4861 gagcgggcgc ctgaacggcg cggcgatcag cgataccctg atgcccacga tgaccctgat
     4921 cgccttcgga tgggtacccg ttttgcttct gacgacctat gtcccggagc tgtccctctg
     4981 gttgcctaga ctgattctgg gcatccggtg acaaaaatac aacacatcaa ggagatgact
     5041 gagatgaacc gagtggtaac gaccgacaag gctcctgcgg cagtaggggc atacagccaa
     5101 ggtgtttacg ccggcccttt ccttttcctg agcgggcaga tcgccctcga cccccaaacc
     5161 ggcaaaatgg ttggtggggg tgacccagcg gccgaaacgg ttcaggtgat gaagaacatc
     5221 cagggagtcc tggaaagcca ggggatgacg atggacaaca tcgtaaaggc cgtcatctat
     5281 gcagccagca tcaaggactt caaagccatt aacgatgttt acaagtcttt cttcccgaac
     5341 ggctacttcc cggcccgctg cttcgtggaa gtcggaggga tggcactgga tgcgaaagtc
     5401 gagatcgacg tgatcgcgac aacggagcaa tcctgaacgg acaggagcgg ctttccttgg
     5461 gaacgcaatc tgcgatcgac atcctgcgaa agcacacttt tttgacccta ggctgtaccg
     5521 acccggtcgc gatcgggctg gcggtggccc atgcctgtcg gatctccgag ggtgaaatcc
     5581 gcggaatcaa ggtacgcctc gacaggaata tctacaagga cgccgtttct gtgggtatcc
     5641 cggggacgca cagttcagga gtgcatctgg cggcagccct cgccgcgatc aaggggcatc
     5701 cggaatcggg actagaactt ttcaaggacg tcgaaccaac agatatcggc ctggccatgt
     5761 cttttctcga aacccacgac atccgtttcg aaattgacga ggaagccgag gggatccgca
     5821 ttgcggctac ggtcacgacc gaaaagggag tttacgaagc actgattgag ggagcgcaca
     5881 accgccttgt cgaaaagagg gcaaacggca aaaccgttta ccgagctgaa aaggcatgtg
     5941 acgaatcaga agataccgac atgaacggcc ttgaccagct cagggacctg accatggagc
     6001 aaatcttcga actactgaaa ggcttgcctg accgggatct ctctttccta ctcgagggcg
     6061 tcgcacggaa cctcgatgcc gcaaaagccg gccttgcgga ccaggccggg atggaattgg
     6121 gcaggtcgct ggaaaagctg cttgagaact cgctcctccc ggattccccc atcaaccgga
     6181 tccgcatttt ggccgcggca gcggcagacg cccggatggg aggcttgaaa actcccatct
     6241 tcggttgcta cggaagcgga aatcacggca ttaccttttt catcaccgtt ggcgagatgg
     6301 ggcggtacct gggcaggagc gatgcggaga tcgcaagagc cctggcgctg ggacttctcg
     6361 ttatcggggt catcaagacg cggaccggga ttctcacgcc ccactgcggc tgcgctgtcg
     6421 ccgcgggcac cggggccgct gccgccatcg tctggctgct tgggggcgac gcgaaacagg
     6481 ttgaaagcgc ggtccagttg atgactgcca accttaccgg aatgctttgt gacggggcca
     6541 agtacggatg cgcccttaag atcgcgactt ccgtcggaat tgcaatcgaa acggcttatt
     6601 tggctctggg aggagtgacc gtccccagcg ggaacggtat cgttggaagg accttccagc
     6661 aaaccatgga caatctggaa accgttacca gcaaagggat gaaagacgtg gaccaagcgg
     6721 tcctcgatgt cctgctcgcg ggacaaaatt aaaaataagg aggcaacagg aatggcttac
     6781 gacgtgttgt ggatttccca agaagaggtc gtttcacttg gcatcaccat gaaggagatt
     6841 gtggacgcgg tcgaagaagg ctggaagcta aaggggcaga agaaagtcga actgcctgcc
     6901 aagatcggtg tccacccccg caaagactgc ttcatccacg ccatgcccag ctgggtaggc
     6961 ggcgacgtgg actgctgcgg catcaagtgg gcggccggtt accccagcaa ccaggcaaag
     7021 ggactgccct acattaatgg cgtctggtgc atgaacgatc ctgaaacggg catcatcaaa
     7081 gctgtcatgg acgcgaactg gatgaccgca acccgcaccg gagctgcttc cggcgtctgc
     7141 gcaaagtacc tagccgaccc gaagagcgag gtcgtctcca tcatcggcct gggagcccaa
     7201 ggacgcacca accttctggc cttcaaggaa acccttcccc agatgaagac cgtgaaggtc
     7261 tacgatattt tcgaaagcca ggtcaccaag ttcgtcaatg agctcggccc ccagcttccc
     7321 ggcgtcgatt tcgtggtcgg aaggaatgtc gaggaagccg tgaaagacgc cgacgtcatc
     7381 gccacctgcg ccccgatcgt ggagaagccc gagcgtttcg tccgctcctc ctggctgaag
     7441 aaggacgtcc tggccatcgc ggtggactac gactccagct tcgacgcgga catcatgaac
     7501 aacggcaact gcttcgtctg cgacgactgg aaccagtacc tctacaccca ggccctcaac
     7561 atctacttcc aggccggcta cccgaagaag gaagaaatct atgctgacat gggcgagatc
     7621 tgcgcgggcg taaagccacc tgtcctccag ggacggcgcg gggcggtcct catgggcatc
     7681 gcgagccacg acatcatgac cggccgcctg atctacagga aagctatcga gaagaaagtc
     7741 ggaacctggg tcaagcttta aagcgtcctg ttcacgtgaa gagcccggaa ttgttgcaat
     7801 tccgggctct ttttccggca aggaggggca aggtatgaac ctcaccgaag aggaacggag
     7861 aatgctggac ggacaattgg gccccttgaa gaggaaagcc ctggagttcg tcgtcagtta
     7921 cgcgaatgtc cttggagccg aaagcctctg cagggtttcc aaggcacacc tgttttgtgg
     7981 agcccaccac tacctcgaag ccgtccgttc gacggacatg gacgaggtta tatcagaaat
     8041 gtggttctgt tcaggagaaa cgatcccctt cgactccgtt ggctgttttg cccagtcgga
     8101 ctgctccccc gtcgatcact tgaactggct gaaaatggga tgcaccgagg aggaattcct
     8161 caagaacgaa cgctacctcg acaggtgcct ccgggccggc gtccacctcg tcgggagctg
     8221 cgttccctac atgaccggct ttatcccctt aagaggagaa cattacgtca caacggaatc
     8281 ccatgccgtc ctgatgatga actccctttg gggagcctgc gccaacgccg acggaatcga
     8341 agccgccttc tgctccgccg tctgtgcccg cactccccgc tgggggatgc atgtacccga
     8401 aagccggaag ggaacccacc tcttctattt gacctgcgaa cccgaaaccg tcgaagactg
     8461 ggacctggtg ggctatgcgg tggggcttcg ccttcctccc cactccgtcc cgatcctcgc
     8521 cgggaacgtg gaaaacgcgg acatggtccg cctgaaatcc tgcttcgcgg ccatggccac
     8581 gacagccggc cccgagcttt gccacatcct cggcgtcacc ccggaagccc ccacacttgc
     8641 ctcctgcctc tcgccgtctg gcctcgaaga cgaggtggaa atcggcccga agcagcttcg
     8701 gcaggcccga gagatccttt gtgacagccg accagggaga gtcgactaca tcagcctggg
     8761 ttgcccgcat tattccatcg accaggtcgg cgaagtcgcc tcatggctga aggggaaaaa
     8821 ggtcttcccg ggaacgaccc tccacgtctg gaccgcgctg ccgatcaggg ccgtggcgga
     8881 ccgatgcggc tacacccgga cgattgagga agccggggga acactgatgg tgggaagctg
     8941 cccgctggtc agcgagaaat ggccgtccgg cacaaccgcc atggccttcg actcggccaa
     9001 gcaggcccac tacatcaagc ctttgaccga tgcgaaggtc tacttcggca gctccggcga
     9061 atgcctggaa gcagccgttt caggccgatg gggcaggtga gggggatgga aacggtcgtc
     9121 atccgcggaa ggggagccgt cccggggatc gccacgggac ccgcgatggt ctgcagggaa
     9181 acgatccagg gctggagtgg catcgacgaa aagactggca aggtgatcga aaaaggacat
     9241 cctgcggaag gacaaacgat acgcggcgtg gtcctcattc tgtccggcgg gaagggttcc
     9301 aacggatggt ccagtcactt tcacgcggca agaatcaggg gtatctcgcc ttgcgccctc
     9361 gttttcccga gaatagactc caggaccgga gtggccgcgg tcgtgtgcgg ggttcccact
     9421 gtcgtcgacc tcgaagagga tccgttcaag atcatcgaga acggagacca ggtcaccgtg
     9481 gacggaaaca gcggaaccct cacgatctac aagggtccgt tcccgacaaa gggggcctga
     9541 cccggtgaac cgctcgagaa caagcgtgat ggaacgcatg taccgttcgg acatggcgct
     9601 ggtgggaacc ttcttcatcc tgatgtggac cgttctgcta tacgtgctcc tccaggtaaa
     9661 ccggatcgca cccgacacga caactcgtac ccttgccact gtcgcggccc tactggttgg
     9721 gggcttcgcc agcggagccc tcgcggccgt ggcgatccac ttgaggaaac acagggaaga
     9781 cctttaccgg gaagattgca aaaactctgg actatgaaac gggaaggaag acgaaagatg
     9841 ccgaggcaaa agggaaagaa ccttttcatg gaagtcttcg acgttctctt tatcatgctc
     9901 ctctgcttcg ccacgctact gtcgacgatg ctcatgaggg ggaaggtcct cgtgggttcg
     9961 ggaagcgggg gcggcatggc ctacaccttc tcggcctgga cattcctcct gacctttttg
    10021 gggctggtgg catacctcgc ctacgtgatt ccccagtcga accgggagct gggagagatc
    10081 gtggaatgcc tttacgggga accgcgggaa aacctgccgc cggaagtgcc gctcgagccc
    10141 gccgcggagg cctcaaaatg agcctttggg aagccctgaa ctggaccgcg tgggggcttt
    10201 ccgccctcat gctggggctc atggccctgg acttcgtccg cgtcgaacgg gaacgcgcct
    10261 cgaagaaagg ggaataggcc atggcggaag aaaaacctca cctttcaaag gtcctgggcc
    10321 cggttcacat ctgggcccta ggcgtgggga tcgtcctcgt gggggagttc atggggtgga
    10381 acttcaccgt cgccaagggg ggaaccctgg gggcgatcat cgcctgctgg gtcatgggac
    10441 tcctctacac ctccctcgtc atggtcaaca ccgagatggg ctccgtcatc ccggaagccg
    10501 gtggacagta cgcgatggcc aagtacttca tgggcccgct ggcggccttc aacgtgggac
    10561 tcatgctcgt gttcgagtac gtcatgctgg aggctgccga tgccctggtg gtgggcgaga
    10621 tcctgaaaac cctgagtccg cacctggaca gcctgccctt catcatcctc accctcgtgg
    10681 ggctcacctg gctgaactac cgcggcgttt acgccaccct caccttcaac ttcgtcatca
    10741 ccgccatcgc ctacatgacg atcttcgtcc tgctcttctc gacgaagttc ttcatcccgg
    10801 gttcaagcct gctgaagcta gccgaattca ccgatgggct tccctacggc tggatcggca
    10861 tcgtgggtgc cttccagttc ggcatctggt tctacctggg catcgaggga acagctttag
    10921 cggccgagga atgccgcagc acgggacggt ccctgccggt gggggccctg accgggatgg
    10981 tgacgctcct catcggggcc accatcacct ggttcgtctg ctctggcttg gtccattcga
    11041 aagaactggg agcctccgtc taccccctct tcgacgcggc cctagccacc ggaatgcccg
    11101 cggtcatcgc actgctcttc gccgggacga tcctctcctg catggcgagc gccaacgggt
    11161 gcatcacgga cgcctcgcgg gcctggttcg ccatggcccg ggacacgctc ataccggaat
    11221 acttcgccgc cgtccacccg aagtaccgga ccccctaccg ggcgatcctc tttcagctgc
    11281 ccatcgccct ggcgttcggc ttcacgggac tgctggacca ggtcatcacc ttctcgatcc
    11341 tgtcggcgct gctggtatac ctcttcacgg cggtcatgat gttcaagttc cggaggatgt
    11401 accccctggg tagcatcgag cgaggctacc tctctccgtg gcacccgtgg accaccctgc
    11461 tgctgctggt cctgaccctg gccaccctct tgggcatgta cttcggctac tggctgaaca
    11521 tgctggcggg attcctcttc tacagcctgg cctcgctttg gttcgccctg caccgctatc
    11581 gcttcgtgga ccgagagaag ctcatctctg aaggagcttc gcggtggccc aggcccgtcg
    11641 gttactaggc ccatgaggct gccgcggtgg gcgatcctcc tggctgcatt cctggttccc
    11701 ggttcgggac acgtcctgct gggaaggccc acccggggcc tcgtgatgct attctggatg
    11761 gtgatcttcg gatacgtgac cttccgactg gcgccgccgc aaacggcctt cctagggagg
    11821 atcggcggcg ggctcgccgt gtgggcactt tccgttctgg atgtcgcgca tatcaatatc
    11881 atggcactta aagttacgaa gtaagaagga ggccaaaaga catgggcaag gtgatcgcca
    11941 tacttggcgg aggcaacgca ggacaggctt ccgccgcgga gttgactctc ttggggcacg
    12001 agtgccgtct ctaccaggac ccccggtttg ccgaagacct gcggcttgtc ctggaaacgg
    12061 ggaaaatccg tctagaggcg gatcccaagg ggggctgcag ggcaacaggc cttgccgcga
    12121 tatcgaaggc aagctgtgac atggccgaaa cggtcagggg tgcggaaatc atcctgtgcc
    12181 accttccagc cttcgctcac gcagacgtcg ctgaacagct cgcggatctc gtggaggacg
    12241 ggcaggtcat cgtccttctg ccgggaaccc tagggacact ggtcttcgtc gagaccttca
    12301 aaaggaaggg cgtcgcggcg gatgtcctga tcgcggaaac caacacgaat tcctacgaca
    12361 ctagggtcct cgggcccgga caagcctacg tctacaagat gaacgaccct ctccagatcg
    12421 gcgtcttccc gtccagccgg accgctgagg cgttggagaa actggacgga gtgtatgcct
    12481 ttgttcccgt gtccgacatc ctggaggccg ctttccacag ccacaacccg gtgatccata
    12541 cccccgcctg catcatgagc gtgagccgca ttgagcggtc cagaggcgat ttctaccttt
    12601 acgaggaagc cttcacccag accgtctgca gggtcaccaa gatgctggac gaggaacgaa
    12661 tggccgttgc ccgcgccttc ggctacgccc cccttactct cgaacagctt ctctcgggac
    12721 tcgaacaacc cggggacctt ttcaaggaaa ccaacgggaa ccccggcctc tgcttcatcc
    12781 ggggaccgga gagcgtgaaa agccgctatt tcaccgagga cggactgaac ggccttgtcc
    12841 cttggtccca gcttgcccgg accgcaggca tccccgtacc gctgatggaa gcctttgcaa
    12901 caatcgaggg ggccgtcatc gaacgggaca tgtggaggga gggccgaacc cttaagacgc
    12961 tcggactgga agggatgaac gtgaccgaga ttcgaaagta cctgagggaa gggaagaaaa
    13021 caggagcttg aatccccctc acacgaattg agcgaaccaa gcccagcatg aaaatggaga
    13081 ggccccggct tttacgccgg ggcctctcca tggaggtagg gtttgagcgg tccgcatccg
    13141 caggtcgtct tctcgccgcc tgaatcttat ctagcctgca ttacggaaat accagcccgt
    13201 ttttcacaat cccgttacac cagcctaagc cgttggttct tgcgatccct tttattcgtc
    13261 cgccttcgac accacaagca gttcccccac ccggtctcgg ggggcgacgt gaatgtcctg
    13321 ctgggcccca acgatctgcc tgtgtgtttc caccgccgtg tccggggtgt tgttttcccc
    13381 cgacaggtga gagaggcaga cccaggagaa caccctgccc gagtccgcca ccagcgcagc
    13441 cgactcctcg ttggaaaggt gccctcccgg ccccgtgatc ctctttttca ggaaggcggg
    13501 atatttcccc cgcttcagca tgccggaatc gtagttgctc tccaggaaca cggcgtccag
    13561 ggaccgcacg acccttccca gcccgtcgaa aacgtgcccc aggtccgtca ggatccccag
    13621 gcgcaggccg ccgtcctcga tcacgaaaac ggaaccttcg accgaatcgt ggggcgtggg
    13681 caccgtctcg acccggacgg atccgaactc aaggcagtcg ccgggagaaa accggcacac
    13741 ggaggccagg cccgcgccga gccccctgca ggccagggcc ccttccgttg ccgccgtgac
    13801 gtagaccggg atgccgtaac ggctgctgat ccccc
//
LOCUS       DAOOVN010000027        13553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_3033_length_13553_cov_15.729664, whole genome shotgun
            sequence.
ACCESSION   DAOOVN010000027 DAOOVN010000000
VERSION     DAOOVN010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 13553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13553
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3033_length_13553_cov_15.729664"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="PLY83_03930"
     CDS             complement(<1..546)
                     /locus_tag="PLY83_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29221.1"
                     /translation="MSWDWILGFTPLSGLILAAVEAQSRVLASGAFCFLNLVVLFFLS
                     LFGSPRARNLFLALLLGMFPVQVLFLKIPPRLAVLHLVVLGSAAGLLWIKARHLDSEK
                     VLFLEFVRKVNGAVNFEEASVLAVRFMRDEFRFPAVCVFLAKEGSDCLERAAAEGFPS
                     ELTKVPKDGSVQGRAYRTRNSQ"
     gene            540..2012
                     /locus_tag="PLY83_03935"
     CDS             540..2012
                     /locus_tag="PLY83_03935"
                     /inference="COORDINATES: protein motif:HMM:NF016158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HOV29222.1"
                     /translation="MTWEPAPSVFSELTYYHIMPGCVEVKEGALVLPDQLFESHPALG
                     RGRPVFFVEEAGDTPAARYRRTCGKEWLAALRQRGFSVDSVRPGREGFAQALLEALAS
                     RGVGTLHAADPVNLEVEDRIRAFCRGNGVRLVIHETPAFLMSRRWLDGFFESRTNNRM
                     AEFYRAVRRETGLLLDSRGKPEGGKWSFDPQNRRRLPKGILIPRAWGTGDPAGILRLP
                     STRREAKAALSDFLENRLDRFGDYEDAMASNEPVLFHSVLSPALNVGLLTPGEVLEAV
                     LRRHRERPVPLNSLEGFVRQVSGWREFVRAMYRRIGARQRAANALGHFAPLPSGFAAG
                     QTGMPPVDTVVSRVNLHGYAHHIERLMVLGNFLLLCETDPAVVNAWFLTRFADATDWA
                     MVPNVFGMSQFADGGLMTTKPYCSGSAYLLRMGDFPEGPWREDWDSLFWRFVNRHRDL
                     FSRNPRTRFLANQLERMSGSRRAELMSRAENLLRKFHESS"
     gene            2131..2637
                     /locus_tag="PLY83_03940"
     CDS             2131..2637
                     /locus_tag="PLY83_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOV29223.1"
                     /translation="MNSQAVSTAEAPENLAFDLQDLFKATPRHPRFLLPLLQDIQSRY
                     RYLPRASLKLVAEHLGIPESRVYAVATFYKALSLTPRGEKTVKVCLGTACHLRGAASV
                     LDALSRSLGIPPGGTTQDMRFTLETVNCLGACALAPVVTVNDRVYGQMTPGRVAELIE
                     KESRHEAD"
     gene            2624..4501
                     /locus_tag="PLY83_03945"
     CDS             2624..4501
                     /locus_tag="PLY83_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOV29224.1"
                     /translation="MKLTSLKAFEEWQALLSEREKGASGVRRVRICAGTGCLANGSAQ
                     VKAAFEAEAIRHGLSIPLEFAAESTGCHGFCERGPLVVIQPGEIFYEKVKPEDVREIF
                     EKTVLRGEVVPRLQYRDPNTKQAARTSAEIPFYARQHRLVLNRCGEVDPFEIEAAVSR
                     GAYRGLARAFSMEPERIVSMIKDSGLRGRGGGGFPTGVKWESARKAPGRHRFVLANGD
                     EGDPGAFMDRSLMEGDPHSVLEGMIIGAYAVGARKGFIYVRAEYPLAVKALQQAIRQA
                     KERGLLGRDILGTGFDFDLEICRGGGAFVCGESTALMASIEGRAGEPRVKYIRSTERG
                     LWNCPTVLNNVETWANVPHIVLNGPEWFRSMGTEKSPGTKIFSLVGKVKNSGLVEIPM
                     GTTLRTMIEDIGGGVLKNRAFKAVQTGGPSGGCLPADALDLPVDFDALTASGSMMGSG
                     GMIVMDERTCMVDVARYFVDFLAEESCGKCTPCREGLKALQRLLHKLCSGRGEAGDGE
                     RLREIGSALADTALCGLGQTAPNPVLSVLRYFPGEIAEHEAEHFCRAGVCKGMYWPRI
                     DPAKCVGCGACKKACAAGAIRGEPRAAHAIDRAHCVTCGACLEACPFSAITAVRREDP
                     E"
     gene            4498..5238
                     /locus_tag="PLY83_03950"
     CDS             4498..5238
                     /locus_tag="PLY83_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOV29225.1"
                     /translation="MIEITIDEKKVFVETGTTVLEAARSAGIEIPTLCAHEGLVREGN
                     CRLCSVEVTERGKTKLVMSCMYPVTREGLEVRTDTDRVRSARRFVLQMLADRNPKAPV
                     LQALCSQYGVVPNVPENVEPELCIRCGRCVRACETNGTSALSFAGRGWDREVTPPFHE
                     APGDCVGCLSCAEVCPTGRITWEEKEGKRSIWGRTFDLVVCERCHEAYATAEQLRHAG
                     TDPETARLCPRCRKMAQQERIRLGTRGA"
     gene            5486..5962
                     /locus_tag="PLY83_03955"
     CDS             5486..5962
                     /locus_tag="PLY83_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOV29226.1"
                     /translation="MEKVLLISPERCIGCGSCEMACSIEKEGEFRPAVSRISVHRFEG
                     GQNVPMTCLQCEDAACMAACKTGALSRNEDGVVVVDKARCIGCRMCVMACPFGNISYS
                     RVTKTAFKCDQCGGDPECVKWCPTKAIEFLPADTVNLQRKRAFSATMAKALEEVKG"
     gene            5962..7770
                     /locus_tag="PLY83_03960"
     CDS             5962..7770
                     /locus_tag="PLY83_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HOV29227.1"
                     /translation="MYGWTGNVLRVNLTDGTFRKEPLRVKDAEAFIGARGLASKYWID
                     DVKDLSVDPLSPRNNLLFATGPLTGTLATSGGRYEVVCKAPLTGALAASNSGGYFGPE
                     LKYAGYDLIIFEGKAPKPVYLYIYNDSVEIRDASALWGKTTHETTDALLAETDPDAKV
                     ACIGPAGEKGVLFAAVINDKHRAAGRSGVGAVMGSKNLKAVAVRGTKGLKVADPAAFL
                     KVVGEARKKLAANPVTSAGLPAYGTNVLVNILNQAGGLPTRNFQTGHFEEADAISGET
                     LASTLLVTKKGCAGCTSACGRVSKTTGQFAGSGEGPEYETAWSFGADCGISDLSAVTK
                     ANFLCNELGIDTITMGSTIACAMELFEKGILTEEETGMDLSFGNAAAMVEMVKKTGLR
                     EGFGDKLALGSWRLASSYGHAELSMTAKKQEMPAYDPRALQGMGLEYATSNRGGCHVR
                     GYLTSPEILGLPQKLDPDSIEEKPAWLKAFQDLTASFDSSGICLFTTFAIGAEELAAQ
                     MAAATGVPYTAEQIMKAGERIWNLERLFNLAVGFTSADDTIPSRLMKEPIPTGPAKGK
                     ISRLPEMLPRYYEVRGWDESGVPTDAKLAELGLARL"
     gene            7774..8046
                     /locus_tag="PLY83_03965"
     CDS             7774..8046
                     /locus_tag="PLY83_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HOV29228.1"
                     /translation="MIRVLFFATIRNLTRENQATVPSAATVRDLIEALSERYGKAFRD
                     EAFEGNEVSGRLIVMVNGRSIAHTGGGSTPLSDGDTVAIFPLVGGG"
     gene            8146..9150
                     /locus_tag="PLY83_03970"
     CDS             8146..9150
                     /locus_tag="PLY83_03970"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HOV29229.1"
                     /translation="MANLEVNWMGLKLASPIIAGSSPLTGTLENLRKLEKAGAGAAVL
                     PSLFEEQLAAAATDLDKGLELGTESYGEALSFFPDMGSYNRGRDLYLELIRTARKELS
                     MPIIASLNGIHPGSWCEFGRAMQDAGADALELNLYVLNTDPAVSASKAERDLAELVRH
                     TVSCVTIPVSVKLAPFYTALPNLALKLKRAGARGLVLFNRFYNPDLDLEKKTPVPRLS
                     KTTQDELWLRLRWSAILYGRVKLDLALSGGVSKAEDAVKALLCGASVVQVTSALYEKG
                     PESIGTLVLGLDRWLEENGYSSAREATGSLSQKSVEDPGAFERANYLQLLRAFRDGPR
                     "
     gene            9147..10505
                     /locus_tag="PLY83_03975"
     CDS             9147..10505
                     /locus_tag="PLY83_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HOV29230.1"
                     /translation="MSPSAFLDPLRLRALKSAPRGTGPVIYWMSRDQRAEDNWALLFA
                     QEMALDAKVPLAVVFILDASFPGATRRSFGFLLRGLAETASTLKDKGILFRLLEGNPP
                     ESLDAFVRQAKAGAVVTDFDPLRVKRGWKEKAASLLPCPLFEVDAHNIVPCWAASEKA
                     EAGAYTLRPKLSRLLPRFLKDFPPLQKHPFGWEEDAPPNDFSGALLRAAHVDGVFEVK
                     ETAPGTKAGLALLREFLQNRLDSYAADRNDPNRNGVSGLSPYLHFGQISPQRVALEAS
                     KTAVSEESRKAFLEELIVRRELADNFCLYNPAYDRTDGFPAWARKTLQEHQTDPREAL
                     YDRSRLENAETEDPLWNAAQAQLVRTGKMHGYLRMYWGKRILAWTSSPEEALEIAIAL
                     NDRYELDGRDPNGYAGIAWCIGGVHDRPWPERPVFGKIRTMTLSGCRRKFDVEKFIRR
                     FL"
     gene            10550..11104
                     /locus_tag="PLY83_03980"
     CDS             10550..11104
                     /locus_tag="PLY83_03980"
                     /inference="COORDINATES: protein motif:HMM:NF017638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 108 family protein"
                     /protein_id="HOV29231.1"
                     /translation="MNRFETCLSFVLSAEGGLSDDPADRGGRTNLGITEGTLARARRD
                     GIAQTERVDSLTRDVAAAIYRAYYWNTCRCKDLPKPLDLAVFDAAVHMGTSAASRQIQ
                     RALNLLGAEPPLAEDGIIGPLSVRAARNLPESWRVRAARIAVAVRSAALLEIASRSVS
                     QRKFLAGWLNRLDRVLERSRREAD"
     gene            complement(11250..11648)
                     /locus_tag="PLY83_03985"
     CDS             complement(11250..11648)
                     /locus_tag="PLY83_03985"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HOV29232.1"
                     /translation="MSVQFGDVFQGLGGGALLGLITLSIVFLALSSIALIIFGVKYLV
                     AILDASGSVGDRKILSPKPVSVESRLPVESKDDGEILAAIAAAVSIVNEAPAKIRSIR
                     PMPEPKAVRISSWKITVRREGFEGIRENGG"
     gene            11859..12752
                     /locus_tag="PLY83_03990"
     CDS             11859..12752
                     /locus_tag="PLY83_03990"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HOV29233.1"
                     /translation="MEIRFFRRCQEMEGLSPSQRAVCNYILENTEKVAFLTVEQLAHA
                     VGTSTATIIRTVAQLGYPSYKALKEEITHLLIDTKPPTRAQLESSWAEDRENTLLQVA
                     RGNAESIEAMLTPYLLEQFREAVSILAEAKRIAIMGLRSNRGLATYFYTILREFHPEV
                     LLVGGFGSDEMYEDLLDLGKTDAFFSFSYSGSAFYAARVIEASRFLRERGISQVLLTD
                     SLKNPLIPHSQAVLHVPCPRDHFSLAPAMSVLDALLAEIGRKTQERSRTKLRSLWKIV
                     TDSGIALPLESGSAKPDIPIP"
     gene            complement(12770..13285)
                     /locus_tag="PLY83_03995"
     CDS             complement(12770..13285)
                     /locus_tag="PLY83_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOV29234.1"
                     /translation="MDILLERRSIRRYQNRPVEEPLVEYCLRAAMAAPSAHNQQPWVF
                     VIIDDRKLLDAIPGFHPYSRMLYEAPMAILVCGDTRDLKSPEFWPQDCAAAVENLLLA
                     ACSRGLGSVWMGVYPKEPIMQGFRRLLELPEHLVPFALVALGYGAEEKGPSDRYDPRR
                     VYRNVWRAENR"
BASE COUNT         2690 a         4259 c         4237 g         2367 t
ORIGIN      
        1 ctgcgaattg cgggtccgat aggctcttcc ctggacgctt ccatcctttg ggaccttcgt
       61 cagctccgac gggaagccct ccgctgcggc ccgctcgagg caatccgaac cttcttttgc
      121 caggaagacg caaacggccg ggaaacggaa ctcgtcccgc atgaaccgga cggcaagcac
      181 ggaagcctct tcgaagttga cggccccgtt gaccttcctg acgaactcga ggaagaggac
      241 cttttcgctg tccaggtggc gggccttgat ccagagaagc cccgcggcgg atcccaggac
      301 gaccaggtgg aggaccgcca gccggggcgg gattttcagg aaaagaacct gcacggggaa
      361 catgcccaaa agcagcgcga gaaaaaggtt ccgggcacgg ggagaaccga acagggacag
      421 gaaaaagagc acaaccaggt tgaggaagca gaacgctccg gatgcaagga cccgggattg
      481 tgcttccacc gccgcgagga tcagaccgga aaggggtgtg aaaccaagga tccagtccca
      541 tgacatggga accagcccct tcagtatttt ccgaattgac ttattatcat atcatgccag
      601 gatgcgttga agtcaaagaa ggcgccctcg ttttaccgga ccagcttttc gagtctcacc
      661 cggccctggg ccggggcagg ccggtcttct tcgtggagga ggccggagac actcccgccg
      721 cccggtaccg ccgaacctgc gggaaggaat ggcttgccgc gttgcgccag cgcggcttct
      781 ccgtcgattc ggtccgcccc gggcgggaag ggttcgccca ggccctgctg gaagcactcg
      841 cctcgcgcgg agtgggaacc cttcatgcgg ccgaccccgt caacctcgaa gtggaagacc
      901 ggatccgggc cttctgcagg ggaaacggcg tgcggctggt gatccacgaa actcccgcct
      961 ttctgatgtc ccgccgctgg ctggacggct ttttcgaaag ccggacaaac aaccgcatgg
     1021 ccgagtttta ccgtgcagtc cgtagagaga ccggacttct cctcgattcg cggggaaaac
     1081 ccgagggcgg gaaatggagc ttcgatcccc agaaccgccg caggcttccg aaggggattt
     1141 tgatcccccg cgcctgggga accggggatc cagcggggat tcttcgcctg ccttctaccc
     1201 ggagggaagc caaggctgcc ctgtctgatt ttctggagaa ccggctggac cgtttcggcg
     1261 attacgaaga cgccatggcc tcgaacgaac cggtgctctt ccactccgtc ctgagcccgg
     1321 ccctgaacgt cggccttttg actccggggg aggtgctgga agccgttctc cgtcggcaca
     1381 gggaaaggcc ggttcccctg aattcccttg aaggtttcgt acggcaggtt tccggctggc
     1441 gggagttcgt gcgggcgatg taccggagga tcggcgcgcg gcagcgcgcg gccaacgccc
     1501 tggggcactt cgccccgctg ccgtccgggt tcgcggccgg gcaaacgggc atgccgccgg
     1561 tcgacacggt cgtgtcccgg gtgaacctgc acgggtatgc ccaccatatc gaacgcctga
     1621 tggtcctggg caacttcctc ctgctctgcg aaaccgatcc tgcggtggtg aacgcctggt
     1681 tcttgacccg gttcgccgac gcgacggact gggccatggt cccgaacgtc tttggaatga
     1741 gccagtttgc cgacggggga ctcatgacga ccaagcccta ctgcagcggc tccgcctacc
     1801 tccttcggat gggggacttc ccggaaggcc cctggcgcga agattgggac tccctgttct
     1861 ggcgtttcgt gaaccggcac cgggacctct tctcccgcaa cccccgcacg cgtttcctgg
     1921 caaaccagct ggaacggatg tccgggtcca ggcgggcgga actgatgtcc cgcgcggaaa
     1981 acctgcttcg aaaatttcac gaatcgtctt aaaaaacctg tcaataaaga cttaaaagac
     2041 agaccgcgaa cgaatgcccg agtgttaaaa tacattatgc aaatacgtct cctatttcac
     2101 attttcaggc aaccccaaag gaggaagacc atgaactctc aggccgtctc gacggccgaa
     2161 gcgccggaaa acctggcctt tgacctgcag gacctgttca aggcaactcc ccggcacccg
     2221 cggtttctgc tccccctcct ccaggacatc cagtcccgct accggtacct gccccgggcc
     2281 tccctgaagc ttgtcgccga acacctcgga atccctgaaa gccgtgtcta tgccgtcgcc
     2341 acgttctaca aggccctcag tctcaccccc aggggggaga aaaccgtgaa ggtctgtctt
     2401 ggcacggcct gccacctaag gggggcggcg agcgtcctgg acgccctctc caggagcctg
     2461 ggcatccccc ccggagggac aacccaggac atgcgcttta cgctggaaac ggtaaactgc
     2521 ctgggcgcct gcgccctggc ccctgtcgtc accgtgaatg accgggttta cgggcagatg
     2581 acgcccggcc gcgtggcgga actgatcgaa aaggagagcc gccatgaagc tgactagcct
     2641 gaaagcgttc gaggaatggc aagccctgct ttcggaacgg gaaaagggcg cctccggcgt
     2701 ccgtcgggtg cggatctgtg ccggaaccgg ctgcctggcg aacggaagcg cccaggtcaa
     2761 ggccgccttc gaggccgagg ccatccggca cggcctgtcg atccccctgg agttcgccgc
     2821 agaatccacg ggctgccacg gattctgcga aaggggcccc ctggtcgtca tccagccggg
     2881 tgagatcttc tacgaaaagg tgaagccgga agacgtccgg gagatcttcg agaagacggt
     2941 cctgcgggga gaggtcgtcc cccgtcttca ataccgggat cccaacacga aacaggctgc
     3001 caggacttcc gccgaaatcc cgttttacgc ccggcagcac cgcctggtgc tgaaccgctg
     3061 cggggaggtc gaccccttcg agatcgaggc cgcggtcagc cgcggagcct accgggggct
     3121 ggccagggcc ttttccatgg aaccggaaag gatcgtttcc atgatcaagg attccgggct
     3181 ccggggaagg ggcgggggcg gctttcccac cggcgtgaag tgggaatcgg cccgcaaggc
     3241 gccaggcagg caccgattcg tcctggccaa cggagacgag ggagaccccg gggccttcat
     3301 ggaccggtcc ctcatggagg gtgatcccca cagcgtgctg gaggggatga tcatcggggc
     3361 ctacgcggtc ggagcccgga aggggttcat ctacgtcagg gccgaatacc cgctggccgt
     3421 caaggccttg cagcaagcga tccgccaagc aaaggagcgg gggctgctgg ggcgggacat
     3481 cctggggacg gggttcgatt tcgaccttga gatctgccgg gggggcggag ccttcgtctg
     3541 cggggaatcc accgccctga tggcctccat cgagggacgg gccggggaac cccgggtgaa
     3601 gtacatccgc tccacggagc gggggctctg gaactgtccc acggtgttga acaacgtgga
     3661 aacgtgggcc aacgtgcccc atatcgtgct gaacggtccc gaatggttcc ggtccatggg
     3721 gaccgaaaag agcccgggaa cgaagatttt ttccctggtc ggcaaggtca agaattcggg
     3781 actcgttgaa attcccatgg ggacgaccct gaggacgatg atcgaggaca tcgggggagg
     3841 cgttctgaaa aaccgcgcct tcaaggccgt ccagaccggg gggccttccg ggggatgcct
     3901 gcccgccgac gccctggacc ttcccgtgga cttcgacgcc ctgaccgctt ccggctccat
     3961 gatgggctcc gggggcatga tcgtcatgga tgaacgcacc tgcatggtgg acgtggcccg
     4021 ctacttcgtg gacttcctcg ccgaggagtc ctgcggcaag tgcaccccct gccgggaggg
     4081 gctgaaggcg ctgcagcgcc ttcttcacaa gctctgctcg ggcaggggag aagctggcga
     4141 cggggaacgc ctgagggaga tcggaagcgc cctggcggac acggccctct gcggactggg
     4201 gcagacggct cccaaccccg tcctctcggt tctccgctac ttcccaggag agatcgccga
     4261 gcacgaggcg gagcatttct gcagggccgg ggtctgcaag gggatgtact ggcctcgcat
     4321 cgacccggcg aaatgcgtcg ggtgcggggc ctgcaaaaag gcctgcgccg cgggggccat
     4381 ccggggagaa ccccgggctg cccacgccat cgaccgggcc cattgcgtca cctgcggcgc
     4441 ctgcctcgag gcctgcccct tttcggccat cacggccgtg cgaagggagg atcccgaatg
     4501 atcgagatca cgatcgacga aaagaaggtt ttcgtcgaaa ccggaacgac ggtgctggaa
     4561 gctgcccgct ccgcggggat tgaaatcccc acgctgtgcg cccatgaggg gcttgttcgg
     4621 gagggaaact gccggctctg ctcggttgag gtgaccgagc ggggcaaaac gaagctggtg
     4681 atgtcctgca tgtaccccgt cacccgggag ggtcttgagg tcaggacgga caccgaccgc
     4741 gtccgaagcg cccggcggtt tgtcctgcag atgctggccg accggaaccc gaaggcgccg
     4801 gtcttgcagg cgctctgctc ccagtacggg gtggttccca acgttcccga gaacgtcgaa
     4861 cccgagcttt gcattcgatg cggccggtgc gtgcgggctt gcgagacaaa cgggacgagc
     4921 gccctctcct ttgctggacg gggttgggac cgcgaggtga cgcccccctt ccacgaggca
     4981 ccgggggact gcgtgggatg cctttcctgc gctgaagtct gcccgacggg acggatcacc
     5041 tgggaggaaa aagaaggcaa acgctccatc tggggccgga cattcgacct cgtcgtatgc
     5101 gaacggtgcc acgaggcata cgcgacggcc gaacagctgc gccatgcggg aaccgacccc
     5161 gagacggccc ggctgtgccc gagatgccgg aaaatggctc agcaggagcg gattcgtctc
     5221 ggtaccaggg gagcttaaag aaccggagaa cccccttcga cccggccggt ctcatgcaga
     5281 cggaccgggc atcgggtggc cggagcgatc ctgccgcctg ccgtgattgc gaagaagggg
     5341 attcttcccc gagtggcggg gaaggatccc ctttcgtgct ttccaagtcc aggcccaagg
     5401 ggcaggggag ggatccaaag gaccgacggg aagaaagatc cggaacgatc gtcaaaccaa
     5461 atacccaaag gggggagcgc tgatcatgga aaaggtgttg ctcatctcac ccgaaaggtg
     5521 catcgggtgc gggagctgcg aaatggcctg ctcgatcgag aaggaagggg aattccgccc
     5581 ggcggtttcg cggatctcgg ttcaccgctt cgagggcgga cagaacgtcc ccatgacctg
     5641 cctgcagtgc gaggatgccg cctgcatggc tgcctgcaaa accggcgccc tgagccgaaa
     5701 tgaagacggc gtcgtggtgg tggacaaggc tcggtgcatc ggatgccgga tgtgcgttat
     5761 ggcatgcccc ttcggcaaca tcagctacag ccgggtcacg aagaccgcct tcaagtgcga
     5821 ccagtgcggg ggcgaccccg agtgcgtcaa gtggtgcccg acgaaggcca tcgagttcct
     5881 gcccgcggac accgtcaacc tgcagcgcaa gcgcgcgttc tcggccacca tggccaaggc
     5941 gctcgaggag gtgaagggct gatgtacggc tggaccggca acgtcctccg ggtgaacctc
     6001 accgacggga cattcaggaa agaacctctc cgcgtgaagg acgccgaagc gttcatcggc
     6061 gcgaggggac ttgcaagcaa gtactggatc gatgacgtga aggatctttc cgtcgacccc
     6121 ctttccccca ggaacaacct gctcttcgcc accggccccc tgacggggac cctggccacc
     6181 tccgggggac gctacgaagt cgtttgcaag gcgccgctga cgggagccct ggcggcttcg
     6241 aactcgggag gctacttcgg gcccgagctg aagtacgccg gatacgacct catcatcttc
     6301 gaaggaaagg cccccaagcc cgtctacctc tacatttaca acgacagcgt ggaaatccgg
     6361 gacgcctcgg ccctctgggg gaaaaccacc cacgaaacga ccgacgccct cctggcggaa
     6421 accgacccgg acgccaaggt ggcctgcatc ggcccggccg gggagaaagg cgtcctgttt
     6481 gccgccgtca tcaacgacaa gcaccgggca gccggccggt caggagttgg agccgtcatg
     6541 gggtccaaga acctgaaggc cgtggcggtc cgtggaacga aagggctcaa ggtcgccgac
     6601 cccgccgctt tcctgaaggt tgtcggtgag gcccggaaaa agctggcggc caatcccgtg
     6661 acttccgcgg gtctgcccgc ctacggcacg aacgtcctgg tgaacatcct gaaccaggca
     6721 ggaggcctgc cgacccggaa tttccagacc ggccacttcg aggaagccga cgcgatctcc
     6781 ggtgaaaccc tcgcttcgac cctgctggtg acgaagaagg gatgcgcggg ctgcacctcc
     6841 gcctgcggga gggtttcgaa aaccaccggc caattcgccg gttcggggga aggcccggag
     6901 tacgaaacgg cctggtcgtt cggcgcggac tgcggcatct ctgacctttc cgccgtcacc
     6961 aaggccaatt tcctctgcaa cgagctgggg atcgatacca tcaccatggg cagcacgatc
     7021 gcctgcgcga tggaactgtt cgagaagggc atcctgaccg aggaagagac cggcatggac
     7081 ctttccttcg ggaacgcggc cgccatggtg gagatggtga aaaagacggg cctgcgggaa
     7141 ggattcgggg acaagctggc cctcggctcc tggaggctgg cttcttctta cggccacgcg
     7201 gagctttcca tgaccgccaa gaagcaggaa atgcccgcct atgacccgcg cgcgctgcag
     7261 ggaatgggcc tggaatacgc cacctccaac cggggcggct gccatgtccg cgggtacctg
     7321 acctctccgg agatcctggg actcccccag aagctcgacc cggacagcat cgaggaaaag
     7381 cccgcctggc tgaaggcttt ccaggacctg acggcgagct tcgactccag cgggatctgc
     7441 ctcttcacca cctttgccat cggggcagag gaactggcgg cccagatggc cgcggccacg
     7501 ggggtgccct acaccgccga gcagatcatg aaagccggcg aacggatctg gaacctcgag
     7561 cgcctcttca acctggcggt gggcttcacg agcgcggacg acacgatccc ttcccgcctg
     7621 atgaaggaac cgatccccac gggcccggcg aagggcaaaa tcagccggct gccggagatg
     7681 cttccgcgct actacgaggt tcgcggctgg gacgaatcgg gcgttccgac cgatgccaag
     7741 cttgcggaac tgggtctggc gcgcctctag cggatgatcc gggttctgtt cttcgccacg
     7801 attcgcaacc tgacccgtga gaaccaggcg acggtgcctt ccgcggcgac cgtccgcgat
     7861 ctgatcgaag cgctcagcga acggtacggg aaagccttcc gggatgaagc cttcgaggga
     7921 aacgaggtct ccggccgcct gatcgtcatg gtcaacggcc gctcgatcgc ccacaccgga
     7981 ggcggaagca ctcccctttc cgacggggac accgttgcca ttttcccgct cgtgggcggc
     8041 ggatagtcga aggaaggcca ggaaggtttc ggggggccac acggcccccc tttttcgtgc
     8101 tatcgtatca ggtggatgat acttttctca aaaggaggga ccgaaatggc aaacctcgaa
     8161 gtgaactgga tgggactgaa gctcgcgagt cccatcattg cgggctcctc ccccctgacc
     8221 ggcaccctgg aaaacctcag gaagctggaa aaggccggag ccggagcggc cgtcctgcct
     8281 tcgcttttcg aggaacagct ggccgccgcc gcgacggacc tggacaaggg ccttgaactg
     8341 ggaacggaat cctacgggga agccctttcc ttttttccgg acatgggaag ctacaaccgt
     8401 gggagagacc tgtacctcga gctgatccgc acggccagaa aggaactttc catgccgatc
     8461 atcgcaagcc tgaacgggat tcaccccggg agctggtgcg agttcggccg ggcgatgcag
     8521 gacgccgggg ccgatgccct cgagctgaac ctgtacgtgc tgaacacgga tcccgccgta
     8581 agcgcctcca aggcggagag ggacctggcg gaactcgtcc ggcacacggt ctcctgcgtc
     8641 acgattccgg tgagcgtcaa gctggccccc ttctacaccg ccttgccgaa cctcgcgctc
     8701 aagctcaaac gagccggcgc cagaggtctc gtcctgttca accgcttcta caatcccgac
     8761 ctcgacctgg agaaaaagac cccggtgccg cgactgtcaa aaacgacgca ggacgaactc
     8821 tggctcaggc ttcggtggtc ggccatcctc tacgggcgcg tgaagctgga tctcgccctg
     8881 tcgggaggcg tgtcgaaagc cgaggacgcc gtcaaagccc tcctgtgcgg cgcttcggtc
     8941 gtgcaggtga cctcggccct ttacgagaaa ggccccgagt ccatcggaac cctcgtcctc
     9001 gggctcgatc gctggctgga agagaacggc tactcgagtg cccgggaagc gaccgggagc
     9061 ttgagccaga agtccgtgga agaccccggc gccttcgaac gggcgaatta cctgcagctt
     9121 ttgagagcgt tcagggacgg tccgcgttga gtccttcggc gtttcttgat cccctgcggc
     9181 tgcgggccct gaagagcgcc ccgcggggga cggggccggt gatctactgg atgagccggg
     9241 accagagggc cgaagacaac tgggcgctgc ttttcgccca ggagatggcc ctggacgcaa
     9301 aagtcccgct tgccgtggtg ttcatcctgg atgcctcctt tcctggcgca acgcgcagga
     9361 gtttcggctt tctgctcagg ggactggctg aaaccgcttc caccctgaag gacaaaggga
     9421 ttctctttcg gctgctcgag ggaaaccctc ccgaaagcct ggacgctttt gtccggcagg
     9481 ccaaagccgg ggctgtcgtg acggatttcg atcccctgcg ggtgaagcgc ggctggaaag
     9541 aaaaggccgc gagcctgttg ccctgcccgc tgttcgaggt cgacgcgcac aacatcgtgc
     9601 cctgctgggc cgcctcggaa aaggccgaag cgggagccta taccctgagg ccgaagctct
     9661 cccgcctctt gccgcgtttt ttgaaggact tccccccgct gcagaagcat cctttcggct
     9721 gggaggagga tgcccccccg aatgatttca gcggggctct ccttcgcgcg gcgcacgtcg
     9781 atggggtttt cgaggtcaaa gaaacggccc cggggacgaa agccggactg gcccttctgc
     9841 gggagttcct ccaaaaccgc ctcgactcct acgccgcaga ccggaacgac ccgaaccgga
     9901 acggggtttc cgggctttcc ccctacctcc acttcgggca gatctcgccc cagcgggtgg
     9961 ccctggaagc ctcgaagaca gcggtttcag aggaaagccg gaaggcgttc ctggaagaac
    10021 tgatcgtgag gcgtgaactg gccgacaatt tctgcctgta caatcccgcg tacgaccgca
    10081 ccgatggctt tccggcctgg gcgagaaaga cgctgcaaga acaccaaacc gatcccaggg
    10141 aggccctgta tgaccggagc cggctggaaa acgcggaaac ggaagacccg ctctggaacg
    10201 cggcccaggc ccagctggtc aggacgggaa aaatgcacgg gtaccttcgg atgtactggg
    10261 ggaaaaggat cctggcctgg acctcttccc ccgaggaggc cctcgaaatc gcaatcgccc
    10321 tgaacgaccg ctacgagctg gacgggcggg atcccaacgg atacgcggga atcgcctggt
    10381 gcatcggggg ggtccacgac cgcccctggc cggaacgccc ggtgttcggc aagatccgga
    10441 ccatgaccct ttccgggtgc cgccggaaat tcgacgtgga aaaattcatc cgccgctttt
    10501 tgtgaaaaac gcctcctgct tccactctta tgagcggagg tggtacggca tgaaccgatt
    10561 tgaaacctgc ctgagctttg tcctgagcgc cgaaggagga ctttccgatg accccgcgga
    10621 tcggggagga cgcacaaacc tgggaatcac ggaagggacc cttgcgcggg cccgccgcga
    10681 cgggatcgcg caaaccgaac gggtcgactc cctcacgagg gacgtggccg cggcaatcta
    10741 ccgggcctac tactggaaca cctgccgctg caaagacctg ccgaagcctc tcgacctggc
    10801 ggtgttcgac gccgccgtgc acatgggcac gtccgccgca tcgagacaga tccagcgtgc
    10861 actgaacctg ctgggggccg aaccgcccct ggccgaggac gggatcatcg ggcccctgtc
    10921 cgttcgggcc gcccgaaacc tgccggagag ctggcgggtc cgagccgccc ggatcgcggt
    10981 cgcggtgcgt tccgctgccc tgctggagat cgcgtcccgc tcggtttccc agcgaaagtt
    11041 ccttgcgggc tggctgaacc ggctcgaccg ggtcctggaa cgaagccggc gggaggcaga
    11101 ctgacaaaag aaagggggag ggcgtctccc ccctccccta ctttttcgca cgaacccgcc
    11161 tgaatacgaa cacggaaaaa acaaccccca tgatcccgag ccagatggct gtcgcggtgt
    11221 tcatttgcct cacctcccgg agcttcattt cacccgccgt tttcccggat cccttcgaaa
    11281 ccttcccttc tgaccgtgat cttccagctt gaaattcgta ccgctttcgg ttccggcatc
    11341 ggccggatcg aacggatttt cgcgggagct tcgttcacga tagaaactgc cgccgcaatc
    11401 gccgccagga tttccccgtc gtctttgctt tccaccggaa gcctgctctc gacggaaacc
    11461 ggcttcgggg acaggatctt tctgtcgcca acggacccgg atgcatccag gatcgcaacg
    11521 aggtatttga ccccgaaaat gatcagggcg atgctcgaaa gcgccaaaaa aacgatgctc
    11581 agggtgatca gacccagcag ggctccaccc ccaagcccct ggaaaacgtc gccaaactgc
    11641 acggacattc ccttgtcccc ctctcaaaag gcaggctctc cacgcgagca tgtaaataat
    11701 ttttcttttt tggctttgta ttggtatgta atttttctta cctataaact ctaacagatg
    11761 atataaaaaa gtcaaacgag agacttggct ccaggaaagg aggggtggat aatcagaagg
    11821 agctgaaaac gaggaagggt cgcagggagg ggaacacgat ggagatccgt tttttcaggc
    11881 gctgccagga aatggaaggg ctttcgccat cccagagggc ggtctgcaac tatatcctgg
    11941 aaaacacgga aaaggttgcc ttcctgacgg tggagcaact cgcccatgcc gtggggacga
    12001 gtaccgcgac gattatccgc acggtggccc agctgggcta cccgagttac aaggccctga
    12061 aggaagaaat cacgcacctc ttgatcgaca cgaagccccc gacgcgggcc caactggaat
    12121 cctcctgggc cgaagaccgg gagaacacgc tccttcaggt ggcccgggga aacgccgaga
    12181 gcatcgaggc catgctcacc ccctacctgc tggaacaatt tcgcgaagcc gtttccatcc
    12241 ttgccgaggc gaagcggatc gccatcatgg gcctgcggtc caacaggggg ctggccacct
    12301 atttctacac gatcctgagg gagttccacc cagaagtgct tctggtcgga gggttcgggt
    12361 ccgacgagat gtacgaagac ctgttggacc tggggaagac ggacgccttt ttctcttttt
    12421 cgtactcggg ttccgccttt tatgcggccc gggtcatcga agcctctcga ttccttcggg
    12481 aaaggggaat ctcccaagtc ctcctgacgg atagtctcaa gaaccccctg atcccccatt
    12541 cacaggccgt tcttcacgtc ccctgcccgc gcgatcattt ttccctcgca cctgccatgt
    12601 ccgtgctgga cgcgctgctt gccgaaattg gccgaaagac ccaggaacga tcccgaacaa
    12661 aactacgcag cctgtggaaa atcgtcacgg attcagggat cgcgctcccg ctcgaaagcg
    12721 ggagcgcgaa accggacatc cccattccct gaaggcagga gcccaaaccc tagcggttct
    12781 ccgccctcca cacgttccgg tagaccctgc gaggatcata tcgatcggaa ggccccttct
    12841 cctccgcgcc gtagcccagg gcaaccaggg caaaggggac aagatgctcc gggagttcga
    12901 ggagcctcct gaatccctgc atgatgggct ctttcgggta gacccccatc cagaccgacc
    12961 ccagccctct cgagcaggca gccagcaaaa ggttctccac ggccgccgca cagtcctgcg
    13021 gccagaattc cgggcttttc aggtctctcg tgtccccgca caccaggatc gccatcgggg
    13081 cctcgtaaag catcctggaa taagggtgaa agcccgggat ggcgtcgaga agcttccggt
    13141 cgtcaatgat cacgaagacc cacggctgct ggttatgggc cgacggggcc gccatggccg
    13201 ccctcaggca gtattccacg agcggttctt ccaccgggcg gttctgatac cggcggatgc
    13261 ttcgcctctc taacaggatg tccattctct catcattcct cccttaaaag cctgtcatgt
    13321 tcctatttta ttccaaccca aggccacgcc attcgtcccc ttgctgatac accagcgggg
    13381 gctttacaag acgatttctt ttgtttagaa ttttccaatc cgctcaccat cgatgatgag
    13441 caacatatta ggctttaaac cacgattacc cagaatttta ttttgggcag tttctatttt
    13501 attgtttcac aaaaaggtgg tgatttattt tcgatgaagc aatagccccg ccg
//
LOCUS       DAOOVN010000028        12952 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_3183_length_12952_cov_13.315422, whole genome shotgun
            sequence.
ACCESSION   DAOOVN010000028 DAOOVN010000000
VERSION     DAOOVN010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 12952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12952
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3183_length_12952_cov_13.315422"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PLY83_04000"
     CDS             <1..192
                     /locus_tag="PLY83_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOV29235.1"
                     /translation="IPGGDTSIARTTGLPPAIAARFILEGKIKTAGVITPVLPEIVDP
                     VLEELQNEGIVLEEKEAEI"
     gene            complement(274..1422)
                     /locus_tag="PLY83_04005"
     CDS             complement(274..1422)
                     /locus_tag="PLY83_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOV29236.1"
                     /translation="MKKALYLICLLVLLAASAAWAAEPIKIGKLAALTGPGATWGEHY
                     KNVSILAVEEINAKGGLLGRPVELIVYDTKGRAEDTVNAARRMILQDKVVAIAGTNYS
                     GLQIAIRPISEQYKVPIMATSATNPAVTVDPEKGTPYPYSFRICFTDPYQGKIMAEFL
                     ANNLKIKEAVIFHDVGSDYAEGMKQFFVATYTKAGGKVAGLYGYREGDVDFRAQITQA
                     KATGAKAVVLPGLYKEMALIIKQCEEMGWKPVFIGGDGYSPAMYEIAGKAMEGTYWVT
                     HIAFDDPILKPLIEKYTKRFGKPPVEIVSITLGYDAMYAIFDAIKRAGKAEGPAIAAA
                     LEKTKGLKLLDFTLNMDPKTHDPLNKPGAILKIENGKEILYTKVTPKN"
     gene            complement(1564..>2055)
                     /locus_tag="PLY83_04010"
     CDS             complement(1564..>2055)
                     /locus_tag="PLY83_04010"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOV29237.1"
                     /translation="LEPKGYIVPKLSGEDMQQVFLTRQKLEGLAAELAASGANPSDRK
                     ALSDLQGEIEEIYRNWDRDRFTDYNERFHNTVARASGNAYVERFLKQCFWRTQLYIYA
                     YDSYFLSPGKSRDTFSKGLYGPTYYEHRAITEAILSGDAAAARETMERHIRSSCEALV
                     RRG"
     assembly_gap    2056..2155
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2229..3551
                     /locus_tag="PLY83_04015"
     CDS             2229..3551
                     /locus_tag="PLY83_04015"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOV29238.1"
                     /translation="MKKVLVFGAGRVAGPAIRTLLEKGHQVTVIDPVVENARRHVGGH
                     PLGKAVQGDALKDGPVLIESDPPDIIVSLLPVALAVPAIDMSIRYGISQVCPIYLNTA
                     RHLEDAIRRAGITVLGEVGLDPGIDHISAARTIRKFHAAGGRIESFWSVCGSLPDHTA
                     NTNPLGYKLTWAPESLVGASVRDATIVKGGKPVSYPGGETFRHYTLEEIEGLGWFEVY
                     ANADSTPYIHQYGMPEVNDVFRGTLRFPGWCDFIAATVSLGLYGQQKKPLEGLTFAGF
                     MRQLFGAPAYAPVLEAVAAFLGIPAHGLVLKQMQWLGLFDERPIPLPEGSPRDVLTLL
                     FYEKLLLLPEERDLVVMQHRYRVFFPDTGRRVLRISTLVNRGGAGEETSIARTTGIPF
                     GIGADLILQGRVREKGLIGPTVPSINDPMLEELESRGIRFTETERELD"
     gene            3566..4474
                     /locus_tag="PLY83_04020"
     CDS             3566..4474
                     /locus_tag="PLY83_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOV29239.1"
                     /translation="MKEKTENELIRPANWWDALPREGYRNLERLDTGQEWYAVYRLDA
                     RTRIIYEDGQFDENVSYLVEGDRQAVLIDTGDNLADIRELVRGMTSLPVAVLNTHRHL
                     DHIGGNYLFDEVWAYDHRVTREAAALGVNHEAALEIIQPGSLRKPVPQPFYENTYCIP
                     PYRVTRWIADGEKIDLGGRVLEAIHTPGHAPDHLMFMEKETGYFWTGDNFYTGCVYTH
                     LPWGNLDVFIDTYEKKILPRLAEARLLLPSHNDPCSRVDVLEEIAALARLARKGGGEY
                     EAGEKGMRKYRGSCFSLVIGADQAEK"
     gene            4496..5464
                     /locus_tag="PLY83_04025"
     CDS             4496..5464
                     /locus_tag="PLY83_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HOV29240.1"
                     /translation="MTEANKSAAEIYDDDYGEKWFSDEASFEEDLPFVWGRKWSVSSE
                     VGRLKACLVRRPGKEIEAVKNPADWRWGDVMDPQKARDQHDAFTQIYRDHGVEVFYVE
                     EQREDRPNAIFMRDNVLGTPEGAILCRQATRFRRGEEAAVAKALAKIGCPIVRTISGK
                     GYFEGACAMWIDRQTVILGSGVRSNAEGIRQVEEVLRPMGVDTIFHYQIPWGHAHLDG
                     LLNPVDRKKILIFPWQTPYDVIEPLRRRGFELIEAPSIEEVKHGMAVNLVALAPGLVV
                     MPAGNPQTQKALERAGVEVIPVDVSELMKGYGSLHCMTAFLEREEI"
     gene            5477..6496
                     /locus_tag="PLY83_04030"
     CDS             5477..6496
                     /locus_tag="PLY83_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HOV29241.1"
                     /translation="MASLKDSPQYYHRVLQRIPPRPTPAFSDEEMQKRVWGRRWGVKN
                     DVGTLKMVLLHRPGDEIRLMTADKYDPSIEALIDDEEQWYFRSNREPDLAKMQAEHDA
                     FAKLLVENGVEVVYMDGGPRNPNAMFTRDTGMVVDGGLIVSRMGPVGKPYGTGRRGEE
                     LYLTRKAAELGMPILRTIAGEGLMEGGSFCLLDERHAAIGMSFRGNESGADQVEDVLK
                     ILGIELIRVPLPGFAMHLDGGIVMVDHDKAVVNVERLPYWFIDRLRELGIELIYADYR
                     DVAYSVNVLAIRPGVVIMDERGTWTRETLEKRGVTVIPTCWEECTKHGGGVHCSSLPL
                     IRELD"
     gene            6747..7166
                     /locus_tag="PLY83_04035"
     CDS             6747..7166
                     /locus_tag="PLY83_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29242.1"
                     /translation="MKRNILVLALFIVLACTGGAALASGSKAFTPPVGSALRQAILAP
                     LRAHLREKLQVEAVFVVRWLKVKEGWAWIETEPRSKDGKNAYEPLFALLVKKEACWRI
                     VDLPPMEEDSPPIDGAYFTKLMEAHPGLPPEIFPWEK"
     gene            7193..7972
                     /locus_tag="PLY83_04040"
     CDS             7193..7972
                     /locus_tag="PLY83_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOV29243.1"
                     /translation="MINTLKRKLECGQAVVGPFLTLNCPDLVELMGLTGFDFLIIDTE
                     HGPMEAESIQNLIRAAELRGITPIVRVSDTRDVTILKTLDVGAHGIQVPQVNSREAAE
                     SVVRSAKYFPAGTRGAAMPRASHYGLLPLDDYLASANRETMVIVHCENRAGLSCIEEI
                     ASTPEVDVVFVGPYDLSQSLGFPGKVGTPQVAGAVDRALKAAQGAGKPAGIFVTSTDE
                     ARMRLDQGFRYIAYSMDSLLFAGACSQAVKALKAHLGCKVE"
     gene            7985..8755
                     /locus_tag="PLY83_04045"
     CDS             7985..8755
                     /locus_tag="PLY83_04045"
                     /inference="COORDINATES: protein motif:HMM:NF022655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LmeA family phospholipid-binding protein"
                     /protein_id="HOV29244.1"
                     /translation="MGRKTAAAALLLLFLGFSLPAAGQEVRSYSGHFTPASRGEELLK
                     ALVDYTDPDAVEMVLDGEPDENWNVRDLFFHVRGGRFAGKIRVEDIALSASFVSFDPP
                     AEGRPLSVKKALRSNLKVSLLESDVNAAIRMFTSEKDGDWNDVSVRFLPPRNVEARGR
                     YHLKNPGLVILAEVSTGLAIRDGVEIWLEDPQLKINRGEQTDTVREAIRKLQPVVDIR
                     KFPFPVTLSVLQAGQGRIEIASRTPPCPFEGIVYRYCR"
     gene            8773..9132
                     /locus_tag="PLY83_04050"
     CDS             8773..9132
                     /locus_tag="PLY83_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HOV29245.1"
                     /translation="MSTEIKPVVSKEETFDRLEIRLGRVLSVEIEPSSPKKAYRMEID
                     FGKFGRKTSVGRFTQHSPEELVGRLVIGVLNFEPRKVGEVLSEVLVLGVQYPKADSGE
                     ATFLTPAAEAKIGGKVF"
     gene            complement(9281..10141)
                     /locus_tag="PLY83_04055"
     CDS             complement(9281..10141)
                     /locus_tag="PLY83_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOV29246.1"
                     /translation="MKRTALKQITLALLVVFPAILAALPALAAGTVRMSSTIGPIDAG
                     IVPALVEAYRTKTGTAVTFEGAGTGATLEKAKSGKFDIVMVHARSLEDQFIAEGYGVD
                     RRDIMYNDFVILGPASDPAGIRGMKSAPAALTRIAIMGAPFVTRGDNSGTHVKEMEVW
                     QAAHIKPEGDWYITYSLGKLGNGPSTAFANRREAYILMDRATYLTMKRDIKLVPLVER
                     DPILLNLITVIRVNPAKFEGLNADAALAFADWLESEEAQLIIKNFGVKKYGEPLFFPN
                     SKSWNSKHGK"
     gene            complement(10176..11009)
                     /locus_tag="PLY83_04060"
     CDS             complement(10176..11009)
                     /locus_tag="PLY83_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOV29247.1"
                     /translation="MKRAWKIATTLVLVFLLLAGAAAAKAPVLRMATTTSTDNTGLLD
                     YLAPIFQKDTGIELQWVAVGTGKALQYGKNGDVDVLLVHDPEAEAVFMKEGYGKSRRE
                     VMFNDFVLVGPAGDPAKVKGKTAAEALKAIAAKKAPFASRGDKSGTHMAELRLWKEAG
                     VAAPDREDWYLSVGQGMIETLAVAGERGGYTLTDRGTYIKYESNFKGNPPLVIVVEKD
                     KKLFNQYSVIVVDPSRAQGLQTDLAERFSAWMASPRGQKVIAGYKMMGKQLFFPNAKA
                     Q"
     regulatory      complement(11142..11260)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            complement(11320..12471)
                     /locus_tag="PLY83_04065"
     CDS             complement(11320..12471)
                     /locus_tag="PLY83_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidE/YbjL duplication"
                     /protein_id="HOV29248.1"
                     /translation="MDFLVKLLNNQLLVLSFAVASGLLLGKVRFRNLSLGTSGALFTG
                     LVLGLLKVKISYELFNWNLMLFVVAVGLLSAEDIVLVVKKYGMKFVVLGVIVTGVGAL
                     CTYAMAHLASGVEPLLVAGTYTGALTSSPGLGAALETTGGNPLVTVGYTLAYPFGVLA
                     VVLFVQLAPAIFGIDVKRERQELASIYRDFYRSGEKDRSVQESIPFSVLSFVVCIVLG
                     ILLGSVTVPVLGSPVSLGSTGGGLIAALALGAFGRLGPLPMRMEKKTLTAIRELSLAY
                     FLAVVGLMAGPQMTDALMEHGFLLVGIGAVSALAAEVAGYLLGRTVWKLNWILLAGAI
                     CGAMTSTPGLGAAIEATGGEECSAGYGATYPMAIVCMVVFTTLLAKALG"
     gene            12615..>12952
                     /locus_tag="PLY83_04070"
     CDS             12615..>12952
                     /locus_tag="PLY83_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOV29249.1"
                     /translation="MEYRIEKDSMGEVKVPADKLYGAQSQRSIDNFRIGWEKMPDEII
                     RAFAVLKKAAALANRKLGVLDEARASAIAKAADEILEGKLAGNFGLSVWQTGSGTQSN
                     MNVNEVLANRA"
BASE COUNT         2481 a         3826 c         4098 g         2447 t
ORIGIN      
        1 atcccgggag gggacacctc catcgcccgg accacggggc tgcccccggc catcgcggca
       61 cggttcatcc tggaagggaa gatcaaaacc gcgggggtca tcaccccggt gcttcctgaa
      121 atcgttgatc cggtcctgga agagctgcag aacgaaggga tcgtcctgga ggaaaaagaa
      181 gcggagatct gacgtttccc aggccgtgaa ggatgcgaac ggcgagggcc ccgtccgggg
      241 tccccgccgt tttttgttcg tttccttggt tttctagttt ttcggggtga ccttcgtgta
      301 gaggatttct ttgccgttct cgatcttcag gatcgccccc ggcttgttga gggggtcgtg
      361 ggtcttcggg tccatgttca gggtgaagtc caggagcttc aggcccttgg tcttctccag
      421 ggccgcggcg atggcgggtc cttcggcctt gcccgcccgt ttgatggcat cgaagatggc
      481 atacatggcg tcatagccca gcgtgatgct gacgatttca accggaggtt ttccgaagcg
      541 tttcgtgtac ttctcgatca ggggcttcag gatggggtcg tcgaaagcga tatgggtgac
      601 ccagtacgtg ccttccatcg ctttcccggc gatctcgtac atggcggggc tgtatccgtc
      661 gccgccgatg aagacgggct tccatcccat ttcttcgcac tgcttgatga tgagggccat
      721 ttccttgtag aggcccggga gcacgacggc tttcgctccg gtagccttcg cctgggtgat
      781 ctgggcccgg aagtcgacgt cgccctctcg gtatccgtaa agccctgcga ctttcccgcc
      841 cgccttggtg taggtggcga cgaagaactg cttcatgcct tccgcgtaat cgctgccgac
      901 gtcatggaaa atgaccgctt ccttgatctt caggttgttg gccaggaatt ccgccatgat
      961 cttgccctgg taggggtcgg tgaagcagat tcggaaagag tagggatacg gggttccctt
     1021 ttcgggatcc accgtcaccg cagggttcgt ggcggaggtg gccatgatag ggaccttgta
     1081 ctgctcgctg atcgggcgga tggcgatctg caggccgctg tagttcgtcc cggcgatcgc
     1141 gacgaccttg tcctgcagga tcatccggcg tgccgcgttg accgtgtctt cggcgcgtcc
     1201 cttggtgtcg tagacgatca gctcgaccgg gcggccgagg agccccccct tggcattgat
     1261 ctcttccacg gccaggatgg agacgttctt gtagtgctcg ccccaggttg cccctgggcc
     1321 ggttaaagcc gccagcttgc cgatcttgat gggctcggcg gcccatgccg cggatgcggc
     1381 cagcaggacc aacaggcaga tcaggtaaag tgctttcttc atgactctcc ccctcctctt
     1441 gaattttcgt ttcctggaat ttcccggcac ggttcgaaaa acgactgttt tccggccggc
     1501 gccggggatc ttggcgggat gttcccgctc ttcgatccgc cgtttcctct ccggggactt
     1561 cgctcaaccc ctccggacca gggcttcgca ggaggaacgg atgtgccgct ccatggtttc
     1621 cctggcggcg gcagcgtcac ccgataggat tgcctcggtg atcgccctgt gctcgtagta
     1681 ggtggggccg tagaggccct tggaaaacgt gtccctggac tttccgggac tcagaaaata
     1741 gctgtcgtag gcatagatgt agagctgggt tcgccagaaa cactgcttca ggaaacgctc
     1801 gacgtaggca tttccgcttg ccctggcgac ggtgttatga aagcgctcgt tgtaatcggt
     1861 gaagcggtcc ctgtcccaat tgcggtagat ttcctcgatt tccccctgca agtcggacag
     1921 ggccttccgg tcggaaggat tcgcgccgga ggctgcaagc tcagcggcca gtccctccag
     1981 tttttggcgt gtcagaaaga cctgctgcat gtcttcaccc gatagcttgg gaacgatgta
     2041 gcctttgggc tccaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngctta
     2161 tgctattttc ctacgagggg aagtcccctg tcaataacaa ttcagggctt tcaactgggg
     2221 gtgaaagatt gaaaaaggtt ctggtcttcg gtgcagggcg cgtagctggg cccgctatcc
     2281 gcactcttct cgaaaagggc caccaggtta cggtcatcga tccggtcgtg gaaaacgcga
     2341 ggcggcacgt gggaggccat ccgttgggaa aggccgtgca gggggacgct ctcaaggacg
     2401 ggcccgtcct gatcgaatcg gatcccccgg acatcatcgt cagccttctg cctgtggccc
     2461 ttgcagttcc cgcgatcgac atgtcgatcc ggtacgggat ctctcaggtt tgccccatct
     2521 acctcaacac ggccaggcac cttgaggacg cgattcgccg tgccggcatc accgttttgg
     2581 gggaggtcgg ccttgacccg ggcatcgacc atatttccgc agcccggacg atccggaagt
     2641 tccatgccgc aggcggaaga atcgaatcct tctggtcggt ttgcggttct ctgccggacc
     2701 acacggccaa caccaatccg ctgggctaca agctgacctg ggcgccggag tctcttgtcg
     2761 gggcgagcgt ccgggacgcc acgatcgtga aaggcggcaa gcccgtttcc tatccgggag
     2821 gggaaacctt ccgccactat acccttgagg aaatcgaagg cctgggttgg ttcgaggttt
     2881 acgccaacgc ggattccacg ccttacatcc accagtacgg catgccggaa gtgaacgacg
     2941 tcttccgggg aacgctccgt ttcccgggtt ggtgcgactt catcgctgca accgtttcgc
     3001 tgggacttta cggccagcaa aagaaacccc tggaagggct gacgttcgcc gggttcatgc
     3061 ggcagctttt tggggctccg gcctatgccc cggttctcga ggcggtggcc gcctttcttg
     3121 gcataccggc ccacgggctg gtccttaaac agatgcaatg gctcgggctt ttcgacgagc
     3181 ggccgatccc gctgcctgaa ggaagtcccc gcgatgtcct gacgctcctt ttttacgaaa
     3241 agctcctcct gcttccggaa gaaagggacc tggtcgtcat gcagcaccgc taccgggtgt
     3301 tcttccccga taccgggcga agggtcctga ggatttcgac gctcgtgaac cggggcggcg
     3361 ctggagagga aacatcgatc gcgcgcacga ccgggattcc attcgggatc ggggccgacc
     3421 tgatcctgca gggccgcgtt cgagagaaag ggcttatcgg accgaccgtc ccgtccatca
     3481 acgacccgat gctggaggaa ctcgaatcca ggggcatccg cttcaccgag acagaaagag
     3541 aactggattg aaaggggaga gagggatgaa ggagaagacc gagaacgagc tgatccggcc
     3601 tgcgaactgg tgggacgcgc tgccgaggga ggggtaccgg aacctggaaa ggctggacac
     3661 ggggcaggag tggtacgcgg tgtaccggct ggatgccagg acccgaatca tttacgaaga
     3721 cgggcaattc gacgagaacg tcagttacct ggtcgaaggg gatcgccagg ccgtcctgat
     3781 cgacaccggc gacaaccttg cagacatccg ggaactcgtc cggggcatga cgagcctgcc
     3841 cgtcgccgtc ctgaacaccc atcggcacct ggaccacatc ggggggaatt acctgttcga
     3901 cgaggtctgg gcctacgatc accgggttac ccgggaagcc gccgcccttg gagtgaatca
     3961 cgaagcggca ttggagatta tccagcccgg cagcctgcgg aaaccggtgc cccagccatt
     4021 ttacgaaaat acctactgca tcccccccta ccgggtcacc cgctggatcg ccgacggcga
     4081 gaagatcgac ctcggcggga gggttctcga ggccattcac acgccgggac acgctccgga
     4141 ccacttgatg ttcatggaga aggaaaccgg ctatttctgg accggcgata acttttacac
     4201 cgggtgtgtc tacacccacc ttccctgggg gaacctcgac gtgttcatcg acacgtacga
     4261 aaagaaaatc ctgccccgcc tggcggaagc tcggctgctt ttgccgagcc ataacgaccc
     4321 gtgctccagg gtggacgtcc tggaggagat cgccgctttg gcccgccttg ccaggaaggg
     4381 cggaggggaa tacgaggctg gggaaaaggg catgcgcaag tacaggggat cctgcttttc
     4441 cctggtgatc ggggccgacc aggcggaaaa atgaaccggg aggaggagaa cggcaatgac
     4501 cgaagcgaac aagtccgcgg ccgagattta cgacgacgat tacggcgaga aatggttcag
     4561 cgatgaagcg tccttcgagg aggacctgcc ttttgtctgg ggccgaaagt ggagcgtgtc
     4621 ttccgaggtc ggaaggctga aggcctgcct ggtaaggcgt cccggcaagg agatcgaggc
     4681 cgtgaagaac cctgccgact ggcgctgggg ggacgtgatg gacccccaga aggcccgcga
     4741 ccagcacgac gcctttaccc aaatctaccg ggaccacggc gtggaggtct tttacgtgga
     4801 ggaacaaagg gaagaccgtc ccaacgcgat cttcatgcgg gacaacgtgc tggggacccc
     4861 ggagggggcg atcctttgcc gccaggccac gcgctttcgc cgcggggaag aagcggctgt
     4921 tgcgaaggcc ctggcgaaaa tcgggtgtcc cattgtccgg accatctcgg ggaaaggcta
     4981 cttcgaggga gcctgcgcca tgtggatcga ccggcagacg gtgatcctgg gcagcggggt
     5041 gcgatccaac gccgagggga tccgccaggt ggaggaagtc ctccgtccca tgggggtcga
     5101 caccattttc cattaccaga tcccctgggg ccacgcgcac ctggacgggt tgctgaaccc
     5161 ggttgaccga aagaagatcc tgatcttccc ctggcagacc ccttacgacg tgatcgagcc
     5221 gctgcgaagg aggggcttcg aactgatcga ggccccgtcc atcgaggagg tcaagcacgg
     5281 catggcggtg aacctggtgg cccttgctcc cgggctggtt gtcatgcccg cggggaatcc
     5341 gcagacccag aaggcgctcg agagggccgg cgtcgaggtc atcccggtgg acgtgtcgga
     5401 gctgatgaag ggctacggat cgcttcactg catgaccgcc ttcctcgagc gggaagagat
     5461 ctaggaggga tcctccatgg catcgctgaa ggacagcccg cagtattacc accgcgtgct
     5521 gcagcgaatc cctccccgcc cgacgcctgc cttttccgac gaggagatgc agaagcgtgt
     5581 ctggggccgc cgctggggcg tgaagaacga cgtcggcacc ctgaagatgg tcctgctgca
     5641 ccgcccgggg gacgagatcc gcctgatgac ggccgacaag tatgacccct ccatcgaggc
     5701 cctgatcgac gacgaagagc agtggtactt ccggtcgaac cgggagcccg acctggcgaa
     5761 gatgcaggcg gagcatgacg cgttcgcgaa actgctcgtg gaaaacgggg tggaggtcgt
     5821 gtacatggac ggcgggcccc ggaaccccaa cgcgatgttc acccgggata ccgggatggt
     5881 cgttgacggg gggctcatcg tttcccgcat ggggccggtg ggaaaaccct acggaacggg
     5941 taggcgaggg gaggagcttt acctgactcg caaggccgca gaactaggga tgcccatctt
     6001 aaggaccatc gccggggaag gcctcatgga gggaggcagc ttctgccttc tcgatgaacg
     6061 ccatgccgcc atcggcatga gctttcgagg caacgaatcc ggggccgacc aggtggaaga
     6121 cgtgctgaaa atcctgggca tcgagctgat ccgtgttccg ctacccggct ttgccatgca
     6181 cctggacggc ggcatcgtca tggtggacca tgacaaggcc gtggtgaacg tcgagcggct
     6241 tccttactgg ttcatcgacc gcctgcgcga actcggaatc gagctgatct atgccgatta
     6301 ccgggatgta gcctactctg tgaacgtgct tgccatccgc cccggcgtcg tgatcatgga
     6361 cgaacggggg acctggaccc gggaaaccct ggagaagcgc ggcgtgacgg tcattccgac
     6421 gtgctgggag gagtgcacga aacacggcgg cggggtccac tgttcctctc tcccactgat
     6481 cagggagctg gactagggat ccagggggac gcacaggtgc ggatctgaag ccccatggca
     6541 gatgaggtct caacggaggt cgattccggc cgtgagttac gacaaggcaa aagccgcgaa
     6601 agagatcggg ctggagggaa aagaaaaaca atttcactga aagaaccgaa aaggagggat
     6661 tccgtaaaag cgggaaccct ccctttttgt tccctttcgg tgcgcggttc ctgtagtaag
     6721 atcgtaaaag aagaaggggg agccgcatga aaaggaacat cctcgttctc gcgcttttca
     6781 tcgtgctggc ctgcacggga ggtgcggcct tggcttccgg ctcgaaggcg ttcactccgc
     6841 ccgtgggaag cgcccttcgg caggcgatcc tggcccccct gagggcccac ctcagagaga
     6901 agctgcaggt ggaagcggtt ttcgttgtcc gctggctcaa ggtgaaagag ggctgggcct
     6961 ggatcgaaac cgagcctcgt tcgaaagacg ggaaaaacgc ctacgagccc ttgtttgccc
     7021 tcctagtgaa aaaggaagcg tgttggagga tcgtagatct tccgccgatg gaggaagatt
     7081 ccccgcccat cgacggggcc tatttcacga agctgatgga agcccacccg ggtcttccgc
     7141 cggagatctt tccctgggag aaatgagacc gaaagaggag gcgacaacga ggatgatcaa
     7201 cacgctgaaa aggaagctgg aatgcgggca ggcggtggtc ggcccgtttc tcacgctcaa
     7261 ctgcccggac ctggtggagc tgatgggcct gacggggttt gacttcctca tcatcgacac
     7321 cgagcacggg cccatggagg ccgagtcgat ccagaacctg atccgcgccg cggaactgag
     7381 ggggatcacg cccatcgtcc gagtttccga cacccgggac gtgacgatcc tgaaaacgct
     7441 ggacgtcggg gcccacggca tccaggttcc ccaggtcaac tcgcgggaag ccgccgaatc
     7501 cgtcgttcgc tcggcgaaat acttccccgc cgggacccgc ggagccgcca tgccccgcgc
     7561 ctcccattac gggctcttgc ccttggacga ctacctggcc tcggccaacc gggagacgat
     7621 ggtgatcgtg cactgtgaaa acagggcggg cctttcctgc atcgaggaga tcgcttcgac
     7681 gccggaggtg gacgtggtct tcgtcggccc ttacgacctg tcccagtcgc tcggtttccc
     7741 cggcaaggtc ggcacccctc aggtcgcagg cgccgtggac cgggccctca aggcggccca
     7801 gggtgcggga aagcccgcgg ggatcttcgt gacgagcacg gacgaggctc gcatgaggct
     7861 ggaccagggg ttccggtaca tcgcctacag catggattcc ctgctgttcg cgggagcctg
     7921 cagccaggcg gtaaaggccc tgaaagcgca tctcggctgc aaggttgagt aaggagaagg
     7981 tcccatggga aggaaaaccg cagccgcagc gcttttgctg ctgtttctcg gcttttccct
     8041 ccccgcggca gggcaggagg tccgttccta cagcgggcac ttcacccccg cttcccgcgg
     8101 agaggaactg ctgaaagccc tggtggacta taccgatcct gacgcggtgg agatggtcct
     8161 ggacggggag cccgatgaaa actggaacgt cagggacctc ttctttcacg tcaggggagg
     8221 gcgctttgcc gggaagatcc gggtggagga catcgccctt tcggcctcct tcgtcagctt
     8281 cgacccgccg gccgaggggc ggccgctttc ggtgaagaag gccctgcgca gcaacctgaa
     8341 ggtgagcctg ctcgaatccg acgtgaacgc cgccatccgg atgttcacct cggaaaagga
     8401 cggggactgg aacgacgtgt ccgtccggtt ccttccgccg cggaacgtgg aggcccgggg
     8461 gcggtaccac ctgaagaacc ccgggctcgt catcctggcg gaggtctcca cggggctggc
     8521 gatccgggat ggggtcgaaa tctggctgga ggacccccag ttgaaaatca accgggggga
     8581 acagaccgat acggtacggg aggccatccg gaaactgcag cccgtggtgg acatccgcaa
     8641 gttccctttc ccggtcaccc tgtcggtgct gcaggcaggc cagggacgca tcgagattgc
     8701 ctctcggacg ccgccctgtc ccttcgaagg gattgtttac cgctactgcc gctagtcctg
     8761 ggggggatgg acatgtcgac ggagatcaaa cccgttgttt ccaaggaaga aacgttcgac
     8821 cgcctggaga tccgcctggg ccgggtgctt tccgtggaga tcgagccctc ttctccgaag
     8881 aaggcctacc ggatggagat cgatttcggc aagtttggca ggaagaccag cgtggggcgc
     8941 ttcacgcagc attccccgga agagctggtg ggccgcctcg tgatcggggt cctgaacttc
     9001 gaaccccgca aggtcgggga ggtcctctcc gaggtgttgg tcctgggcgt gcagtacccg
     9061 aaggccgaca gcggcgaagc gacctttctg actccagccg cggaggcaaa gatcggcggc
     9121 aaggtcttct aataaagcct tttgccagaa gcggcaaaaa agccccccag gggacgggga
     9181 aaagatgacc gcctaccggg gggcttctta atttaggccg agtatgaaat ttcagtcagg
     9241 ttttcctcgg attcctttct tgcgcatcgt gccgggtttg ttactttccg tgcttcgaat
     9301 tccaggattt cgaattgggg aagaaaaggg gctctccgta tttcttcacg ccgaagttct
     9361 tgatgatcaa ctgggcttct tcgctttcga gccagtccgc gaaggccagg gccgcatcgg
     9421 cgttcagccc ctcaaacttc gcagggttga cccggatgac ggtgatgagg tttaacagga
     9481 tcgggtccct ttccacaagc gggacaagct tgatgtccct tttcatggtc aggtaggtgg
     9541 cccggtccat gaggatgtag gcctcccggc ggttggcaaa ggccgttgag gggccattcc
     9601 cgagcttgcc cagggaataa gtgatgtacc agtctccctc cggcttgata tgggcggctt
     9661 gccagacttc catctccttc acgtgggtgc ccgagttgtc tccccgggtg acgaacgggg
     9721 ctcccatgat ggcgattcgg gtcagggcag ccggggcgct cttcatgccc ctgattcccg
     9781 cggggtcgga agcggggccg agaatgacga agtcgttgta catgatgtcc cggcggtcca
     9841 cgccgtaacc ttcggcgatg aactggtcct ccagggagcg ggcatggacc atgacgatgt
     9901 caaattttcc agacttggct ttttccagcg tggctcccgt tccggcaccc tcgaaggtca
     9961 ccgcggttcc agtcttggtc ctgtaggctt cgacgagggc agggacgatt cccgcgtcga
    10021 tggggccgat ggtgcttgac atacggaccg ttcctgcggc aagagcgggc agggcagcca
    10081 ggatagccgg aaagacaacc agcaacgcca gcgtaatctg tttcaaggcc gtgcgtttca
    10141 tcgatcgttt ccttctctct cttagggtgc agggcttact gggcctttgc gttcgggaag
    10201 aagagctgct tgcccatcat cttgtacccg gcgatgacct tctgtccgcg cggggaagcc
    10261 atccaggcgg aaaagcgctc ggccaggtcc gtctgcaggc cctgggcgcg gctcgggtcc
    10321 accacgatga cgctgtactg gttgaagagc ttcttgtctt tctccacgac gatcacgagc
    10381 ggggggttcc ccttgaagtt ggattcatac ttgatgtagg tgccgcggtc tgtgagggtg
    10441 tagccgcccc gttctcccgc cacggcaagc gtctcgatca tgccctgacc cacggacagg
    10501 taccagtctt ccctgtcggg ggccgccacg ccggcctcct tccacaggcg gagttccgcc
    10561 atgtgggtcc cggacttgtc tccccggctg gcaaagggtg cctttttagc ggcgatagcc
    10621 ttcagggcct ccgccgccgt tttccctttc accttcgcgg gatcgccggc aggccccacg
    10681 agaacgaaat cgttgaacat gacctcgcgg cggctcttgc cgtacccttc cttcatgaaa
    10741 acggcctcgg cctcggggtc gtgcaccagc agcacgtcca cgtccccatt cttgccgtac
    10801 tgcagcgcct tgcccgtgcc gaccgcgacc cattgaagct cgatccccgt gtccttctgg
    10861 aagatggggg ccaaatagtc caaaaggccc gtgttgtccg tgctggtcgt cgtggccatc
    10921 ctcaggacag gggccttggc cgccgcggcg ccggccagca gcaggaaaac gagaaccagg
    10981 gttgttgcga tcttccatgc gcgcttcacc aacttaaccg cctcctaaag caagatttgc
    11041 cccagaaact aggggggagg ggccctgaga agggccccta ggggctcgag aaagcgggag
    11101 aaaagaaaac aggccgcccc cgtggtcaga aggcggcctg tttctaagcc cgcgaatctc
    11161 ctttccaaac gggaggcttc cccgtttcca gggaaaccca ttggcctgcc gccgtgggga
    11221 gatcccttta ggaccggcag gctcggagag taaatttaat tttgcgcaat tccttttcta
    11281 ccagaaaaga ttcctccctg caagaggggg aaaggagatc taacccaggg cctttgccag
    11341 gagggtcgtg aagaccacca tgcagacgat ggccatgggg taggtggctc catagccggc
    11401 gctgcactcc tccccgccgg tcgcctcgat ggccgctcca agacccgggg tgctggtcat
    11461 ggccccgcag atcgctccgg ccagcaggat ccagttcagc ttccagaccg ttctgcccag
    11521 gaggtatccc gccacctccg ccgccagggc ggaaaccgcg ccgatgccga cgagcaggaa
    11581 gccgtgctcc atcagggcgt cggtcatctg gggcccggcc atcagtccca cgaccgccag
    11641 gaaataggcc agcgaaagtt cgcggatcgc cgtcagggtc tttttttcca tcctcatggg
    11701 caaggggccg agacggccga aagcccccag ggccagggcg gcgatgagcc cgcccccagt
    11761 gctgccgagg gagacggggc tgcccagcac cgggacggtg acgctgccga gcaggatgcc
    11821 caaaacgatg cagacgacga aggacagcac ggaaaaggga atcgattcct ggacggatcg
    11881 gtctttttct cccgagcggt aaaaatccct gtagatcgat gccagctcct gccgttcccg
    11941 cttcacgtcg atcccgaaga tcgccggagc aagctggacg aacaggacca ccgccagcac
    12001 cccgaaggga taggcgagcg tatagcctac cgtaaccagg ggattgcctc ccgtggtttc
    12061 cagggccgcc cccagtcccg gcgaactggt cagggccccc gtgtaggttc cggccaccag
    12121 caggggttca acgccggacg ccaggtgtgc catcgcgtag gtgcagaggg caccgacccc
    12181 ggtgacgatc acccccagaa cgacgaactt catcccgtac tttttcacca ccaggacgat
    12241 gtcctccgcc gagagcagcc ccaccgccac gacgaagagc atgaggttcc agttgaacag
    12301 ctcgtaggaa attttcactt tcagcagccc cagaaccagg ccggtgaaaa gggccccgga
    12361 agttcccagg ctgaggttcc tgaaccggac ctttccgagc agaaggcccg aggcgaccgc
    12421 gaagctcagg acaagaagct ggttgttgag aagcttgacc aggaaatcca cgatgaacgt
    12481 ccctcccgac cttccgcgcg agcggtgcga tccgacgtgc ctattctacc ggattcctcc
    12541 cgcccttgaa ttcatttctc cctttcccta acatggaaag gccccgatcg ggtttgcgaa
    12601 ggaggcctgc cgaaatggaa taccgaatcg agaaagactc catgggagaa gtgaaggttc
    12661 ccgcggacaa gctttacggc gcccagtccc agagaagcat cgacaacttt cggatcggct
    12721 gggaaaagat gccggacgag atcatccgcg cgtttgcggt cctgaagaaa gccgcggccc
    12781 tggccaaccg gaagctcggc gtccttgacg aggcgagagc ctcggccatc gcgaaggcgg
    12841 ctgacgagat cctggaagga aagctggcgg gcaacttcgg gctttccgtc tggcagaccg
    12901 gaagcgggac ccagtcgaac atgaacgtga acgaggtcct ggccaaccgg gc
//
LOCUS       DAOOVN010000029        11945 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_3502_length_11945_cov_13.807065, whole genome shotgun
            sequence.
ACCESSION   DAOOVN010000029 DAOOVN010000000
VERSION     DAOOVN010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 11945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11945
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3502_length_11945_cov_13.807065"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(264..1388)
                     /gene="recA"
                     /locus_tag="PLY83_04075"
     CDS             complement(264..1388)
                     /gene="recA"
                     /locus_tag="PLY83_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOV29250.1"
                     /translation="MAKNGKGALTREDILEQALEDIKGKFGDGAIMRLGDQTNVAVDV
                     IPTGVLPLDVALGVGGLPRGRVVEIFGPEGSGKTTLALHAIAEAQKAGGIAAFIDAEH
                     ALDPRLAASLGVDVPSLYLAQPDSGEQALYVLESLVRSGAVDIIVVDSVAALTPQAEI
                     DGKIGDTQVGLQARLMSYALRRLTAAISKSRAVVVFINQLRAQISTMGYGGPQETTTG
                     GRALKFYSSLRIEVRRGKAITQGDDTLGHELWIKVVKNKLAPPFRTAHTSLIYGKGVP
                     AEMAVVDMAIDLEVIRRKGSWLAYKGENLGQGKEKVSKYLLETPALMEEIKKEILEKA
                     GQGDALLVPKAPAGEPAAVETKLTEPVVEEELLELEEEDI"
     gene            complement(1412..1993)
                     /gene="thpR"
                     /locus_tag="PLY83_04080"
     CDS             complement(1412..1993)
                     /gene="thpR"
                     /locus_tag="PLY83_04080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOV29251.1"
                     /translation="MGERTRSFLCIEPEPALRATLGEWLQSLRRLAPRLKWVQPDAVH
                     LTLKFLGEQPPEMLSELRNRLPGVLSANGHRTFYLELSGVGAFPNYRAPRVLWMDLSG
                     ETASLNDLWKTVENLAVSIGFPPETRPFRPHLTLARIKDPGDFTLALAKNLSESDPPM
                     GRWKVGFVTLMRSELRPGGPLYSPIATFPLFRV"
     gene            complement(1997..3205)
                     /locus_tag="PLY83_04085"
     CDS             complement(1997..3205)
                     /locus_tag="PLY83_04085"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HOV29252.1"
                     /translation="MANGVALLAVGDEMLSGLRSDTNCSWLASRLHDAGVPVRGIQVL
                     PDDEDVILETVAGWVGKVEFLVLSGGLGPTHDDRTRFALARYLGVPLRQDDPTYDRIV
                     ARYEEPLRSRVEASRVPQALIPEGARGLPNPQGSALGIAFSARGTEVFSFPGVPWEFR
                     AMAETFFLPRIEARGTWKQVVVAGWPESLLAEKFEGLFSKPGLHVSILPSPGLVTLVF
                     RGSPEDVTSAVARVRGELPSDCLEEGETEMPVAVVARSARAGLTLACAESCTGGLVGA
                     AITDVPGASRVFQGTAVCYSNEAKIRLLGVKPGTLENHGAVSEECALEMAAGARGLFG
                     SDLSVAVTGIAGPEGGTREKPVGTVWFAVSGPKTVKAFKRSLAGDRAHIRRWSAAIAL
                     EILWRTLKEG"
     gene            complement(3224..3694)
                     /locus_tag="PLY83_04090"
     CDS             complement(3224..3694)
                     /locus_tag="PLY83_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HOV29253.1"
                     /translation="MGYRMPETWEGKIGTVGGLGYLTGMPGTVGSAAAFLVAVVHPVP
                     AWFILLFAGLSVWASGRYARQVGREDPPEVVADEVVGTWVAIWGLPFGYWLPALCLFR
                     VIDILKPFPVSTSERLPGGWGIVADDFVGGVLTNGLIRSAAWLFFGGGLQLFFR"
     gene            complement(3684..4973)
                     /gene="rimO"
                     /locus_tag="PLY83_04095"
     CDS             complement(3684..4973)
                     /gene="rimO"
                     /locus_tag="PLY83_04095"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOV29254.1"
                     /translation="MLSLGCAKNQVDSERLANALVAWGYELVETVEEAGVAIVNTCGF
                     IRPAAEESIRAILQMEELKANGRLERIGVVGCLLNRYGETLRKELSSVDFWAEAEDWG
                     TLGERLGQTEPYCREGRKALPGTLPWTRYLKIAEGCDNRCSYCTIPSIRGGVRSLPPD
                     FLAKEAGELARQGAREICLVAQDLTAWGVDLFGKRSLHLLLRELESAVPEGTWLRLLY
                     LHPSGIDDALLEQVLSSSRILRYLDVPIQHADAGVLRSMNREIPPGRLREIFGKIRGA
                     DPDFALRTTVMVGHPGEDEAAFGNLLDFIASVQFDRLGAFEFSPEEGTLSASLRNKVP
                     RKVKKRRMDRLMRLQEEISFERQGRFEGRSLEVLVESVEPGEDLALGRSYREAPEVDG
                     LVEIRGGGALQPGCFVTVTVKEALEHDLVAEVQGNGV"
     gene            complement(4990..5847)
                     /locus_tag="PLY83_04100"
     CDS             complement(4990..5847)
                     /locus_tag="PLY83_04100"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HOV29255.1"
                     /translation="MNDQTLRELGAYLCWKRKEKGKTLEDVSAETRLRVEILRAIEKG
                     EIQALPAPVYARGMVRLYLEYLGSSELLPQYEEFFQSQVRAEPPEGSFAKPAVTRGFR
                     RRDNRWAVVLLALAVLLAGYLLWQQKGVLGKAIEKKAEQATVAAQLQTPPATQTSLDR
                     PSVPLETQTSAGDPAGLQPQSDDLSWLPGHGPGTGSSGESVSGRLEITALESCWIQVT
                     REGETLFRGTLKKGESKSFPSEKEIRVRYGKPTGVAVRWMGKDLGRPSTQQKVVTVLY
                     KPDGSFTVR"
     gene            complement(5840..6529)
                     /gene="rpe"
                     /locus_tag="PLY83_04105"
     CDS             complement(5840..6529)
                     /gene="rpe"
                     /locus_tag="PLY83_04105"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOV29256.1"
                     /translation="MNGQRIAPKIAPSLLSADPLRIWEGIQSLAGEYDWIHLDIMDGH
                     FVPNLSYGPALLKALRSALPEAVLDVHLMVEPPEDFIDSFLRWKPDYLVVHQEATPHL
                     HRVLQAIREGGSRPGVAVNPATCVEALKPILPFVDLVLVMSVNPGFGGQAFIPEVLAK
                     TRTLCQLREAQGLEFLVEMDGGIGLSNVEEVVKSGCDVIVAGNAIFGEADPALTARLL
                     RKKAREAGSGE"
     gene            complement(6526..7677)
                     /locus_tag="PLY83_04110"
     CDS             complement(6526..7677)
                     /locus_tag="PLY83_04110"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOV29257.1"
                     /translation="MKWIRWTMLLLVLVIAGSAAAAFYFVFMGGRTIPMPAVEGMISS
                     EAVETLQKAGLLVRVDQVTSQLPAGTVVSQWPGASEKVAKGRLVTLKVSRGGEKKPLP
                     DVRGMEFNEAKAKLAEAGFQVGDVLRVADPAKPAGSVLAQSPAAPAMVGPETRIDLLL
                     SRGTGVEGSAVSVPDVVGQPEGAARALLEAAGLGVEIRYLVSQETAPGIVVSTTPKTG
                     AAVPAGSRVALTVATPSNVSQAKAPSPPAEEDPEAPPKPKTVEKKQENSAGTPEAPAR
                     KVAEQPEQKPVSEQAVPPKKEVPAAASPASAKEEETRHTAKVRYQVPPLSRPLSLRIE
                     VVDPEGTRTVLEREVKGGESIAVNVPYVREAAVTVYLGGEFVWQDRYRP"
     gene            complement(7691..9013)
                     /locus_tag="PLY83_04115"
     CDS             complement(7691..9013)
                     /locus_tag="PLY83_04115"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HOV29258.1"
                     /translation="MRGIEAALECRREVRKGAFAAEALRKVGAQLDPPDRVLASSLLY
                     ATSRRESLWKHLLGLFLRKPLHALSAETADVLMVGAAGLIELRHFSPQVLVSALVGHV
                     KAGRFPHEAGLVNAVLRRVREEAPQVLAKLASSADAKDQCLFLGIPSWAGRRWIEEWG
                     KEEARNLWKLSSMKPYLSVRLNRPEKEQEFLTALERAKIRGWPSPLLRESCRLSSNPF
                     PPSLPGFAEGWVTPQTESSMLVVEAFLELLDGGPVLEMCSGRGVKSGQLLCRLPEGIP
                     VESWELSPGKVASAKAEMHRIGVARRAVFKIGDARTLEPERAPRAVFLDAPCSGSGTW
                     KRHPESKWRLSPERLAQMAGLQAALLERAFRLVQPGGIVVYCTCSLFREENEQVVGSV
                     LGFGGDLVELPLSLPGTVSVKGKPYGRILWPKLPWIDGFYVAAFMKRA"
     gene            complement(9167..9862)
                     /locus_tag="PLY83_04120"
     CDS             complement(9167..9862)
                     /locus_tag="PLY83_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOV29259.1"
                     /translation="MFPMFDPTFVLLIPALLLGLWAQAKVQGAFATYSQVPSRRGVRA
                     CDVARMIMNRFGLSGIAIERVPGNLTDHYDPRSKVLRLSEGVYSSSSLAAIGIAAHEV
                     GHAIQDAEDYAPIRVRNAIVPVASLTSWLAFPLFFIGLLMRTPFLMDLGILFFLGVIV
                     FHLVTLPVEFDASARALRLLADTGTLDMDEIGGARAVLRAAALTYVAAAVMAVAQLFR
                     LLLLRGMVGGRRD"
     gene            complement(9962..11098)
                     /locus_tag="PLY83_04125"
     CDS             complement(9962..11098)
                     /locus_tag="PLY83_04125"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOV29260.1"
                     /translation="MPDRRLPTLDLKRNYARVREEIREAVDKVLESQHFILGPEVRAF
                     EEEAARMVGAPYAVGCASGTDALVLALMALKVGPGDEVITTPYSFFATASCITRQGAR
                     PVFVDIDPETYNLDIAQVLEKITPRTKAVIPVHLFGQMVPVERLLPELQEKGIALVED
                     TAQAFGACRQVGDRIYRAGAVGDLGCISFFPTKNLGAYGDAGMITSTREDLARSLKSL
                     RVHGSTETYFHELVGINSRLDEIQAAILRVRLRHLQEWNEERRQAAARYALLFREYGL
                     LEQVRPPVEASGNTHIYHQYVVRCRDRDTLQAYLEKEGISTRVYYPLCLHLQPCFSGL
                     GYAEGDFPEAEALSRDCLALPIFPELKAEEQEWLVSAMAEFYHR"
     gene            complement(11243..11908)
                     /locus_tag="PLY83_04130"
     CDS             complement(11243..11908)
                     /locus_tag="PLY83_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOV29261.1"
                     /translation="MREARFYTTSADYVYQDLRHRIITKQLQPGQRLPEVNIAVQMGV
                     SRTPVREALRRLASEGLVTIIPNSGARLACPSQKEMENAYVLREHLECLAIRLAAERI
                     TERQLRRLEDSVIEEERAFHERDLKLYLEVNENFHRTIAESSGNRILAEYVENILART
                     NVYIVFYDPFYDIDTNPSIPQHRAIIAALAERSPEKAEALMREHLRFSMEGLQLPKEP
                     EEF"
BASE COUNT         2325 a         3898 c         3340 g         2382 t
ORIGIN      
        1 ttctcctctt cacgccttcc ctctctcttc ccttacgctt gtcaaggtgc tgttgtgaaa
       61 tggtgccttg ctgctgaacc cctctctcga ggggcaaggg gcataataca tcgcctgccg
      121 tatcctgtca acacctacgg gaggttttcg ttccgatttc ctgactgcct tccgtgatac
      181 aaacgctgac aaagattctt tccgccttcg gaatggtatc gtagtgcttt tgattcaggc
      241 ttctttcggg tttaattccc gttctagatg tcttcttctt cgagttccag cagttcttcc
      301 tcgacaacag gctcggtcag tttcgtctca acggcggcgg gttcgcccgc tggtgccttc
      361 gggacaagca aggcatcccc ctgtccggcc ttttccagga tctctttctt gatctcttcc
      421 atgagcgccg gggtttccag gaggtatttc gaaacctttt ccttcccctg ccccaggttt
      481 tcccccttgt aggccagcca ggatcctttc cgcctgatca cttccaggtc gatcgccatg
      541 tccaccacgg ccatctcggc tggaacgccc tttccataaa tcagggacgt gtgagcggtc
      601 ctgaagggcg gcgcgagttt gttctttaca accttgatcc agagctcgtg cccgagggtg
      661 tcgtccccct gggttatggc ttttccacgc cgaacttcta tccgcaggga gctgtagaat
      721 ttcagtgccc tccctcccgt tgtggtttcc tgcggccccc cgtagcccat tgtggagatc
      781 tgggctctca actggttgat gaaaacgacg accgcccggc ttttggagat cgctgccgtc
      841 agccttcgaa gggcgtatga catgaggcgg gcctgaagcc ccacctgggt gtctccgatt
      901 ttcccgtcga tttctgcctg gggggtcaga gcggccaccg aatccaccac gatgatgtcc
      961 accgcaccgc tccgaacgag ggattcgaga acataaagcg cctgttcgcc gctgtcgggc
     1021 tgagcaaggt acagtgaagg cacatcgacc cccaggcttg cggccagcct cgggtcgagc
     1081 gcatgctccg cgtcgatgaa ggcggcgatt cctcccgcct tttgggcttc ggcgatggca
     1141 tgaagtgcga gcgtggtttt tcccgaccct tccggtccga aaatctcgac gacccgtccc
     1201 cggggaaggc ctccaacgcc cagcgcaaca tccagcggaa ggacccccgt ggggatcacg
     1261 tccacggcga cgttcgtctg gtcaccaagg cgcatgattg ccccgtctcc gaatttcccc
     1321 ttgatatcct caagggcctg ctcgaggata tcttcccgcg ttagagctcc tttgccgttc
     1381 tttgccattc caggatggca cctcctttgc cctaaaccct gaaaagcgga aaagtcgcga
     1441 tgggagaata gagaggaccc cccggccgca actcgcttcg catcaatgtt acaaatccca
     1501 ctttccagcg ccccatcggg ggatcggatt cactcaggtt tttcgccagc gccagggtga
     1561 aatccccggg atccttgatc ctggccagtg tcagatgggg ccggaacggt ctcgtttccg
     1621 gcgggaaacc aatgctgacc gcaaggtttt ccacggtctt ccaaagatcg ttgagggagg
     1681 cggtttcgcc gctaagatcc atccagagca ctcgaggagc cctgtaattc ggaaacgccc
     1741 ccactccgga caattcgaga tagaaggtcc tgtggccgtt ggcggaaagg acaccgggaa
     1801 gcctgtttcg cagctccgaa agcatttccg gcggctgctc gcccaaaaac ttgagggtca
     1861 ggtggactgc atccggctga acccacttga gccttggcgc cagtctcctg agcgattgga
     1921 gccattctcc gagggtggcg cggagagccg gttccggttc aatgcagagg aacgaccgcg
     1981 tcctttctcc catcggctat ccctccttca gggttctcca caagatttcc agagcgatcg
     2041 ctgctgacca tcgccggatg tgggcgcgat cgccggccag gctgcgtttg aaggctttca
     2101 cggtttttgg cccggaaacg gcaaaccaga ctgtcccaac cggcttctcc ctggtccccc
     2161 cttcagggcc ggcgatgccc gtgacagcca cggaaaggtc cgatccaaaa agtccgcggg
     2221 cgccggcggc catttccagg gcacactcct cgctgactgc accatgattt tcaagagttc
     2281 ctggtttgac cccgagcaag cggatcttcg cttcgtttga atagcagacc gcagttccct
     2341 gaaaaaccct ggaagctccc ggaacgtccg tgatcgcagc ccccacaagg cctccggtgc
     2401 aggattcggc gcatgcaagg gtgagacccg ccctggcgga gcgggccaca accgccacgg
     2461 gcatttccgt ttccccctct tccaggcagt cggaaggaag ttcccccctg accctcgcga
     2521 ccgctgaggt cacgtcttcg gggcttcccc tgaacaccag cgtgacgagc ccgggggatg
     2581 gaaggatcga aacatgcagg cccggcttcg agaacaggcc ctcgaacttt tcggccagga
     2641 ggctttcagg ccagccggca accacgacct gcttccaggt cccccttgcc tcgatccgcg
     2701 gcaggaagaa ggtttccgcc attgcgcgga attcccaggg caccccgggg aaagaaaaca
     2761 cttccgttcc ccttgcggaa aaagcaatcc ccagcgcgga gccctgcggg ttgggcaacc
     2821 ccctggcccc ttctggaatc agggcctggg ggacacgtga agcctcgacg cgacttcgaa
     2881 ggggttcttc gtatcgggcg acgatccggt cgtaggttgg gtcgtcctgg cggagcggaa
     2941 cacccaagta gcgggccagg gcgaaccgag tccggtcgtc gtgggtcggt ccaagcccac
     3001 ccgacagcac caggaattcg actttgccaa cccagccggc aacggtttcg agaatgacgt
     3061 cctcgtcatc gggaagcacc tggattccac ggacgggcac cccggcatcg tgcagcctcg
     3121 atgccagcca cgagcagttc gtgtccgaac ggagaccgct tagcatctca tccccgaccg
     3181 ccagaagggc cactccgttt gccattccca gcacctctct tctttaccgg aaaaaaagct
     3241 gcagcccgcc cccgaaaaag agccaggcgg ctgagcgaat cagtccattg gtcaaaacac
     3301 cgcccacgaa atcgtcggca acaatccccc agccgccggg aagccgttcc gaagtcgaaa
     3361 ccggaaaggg tttgaggatg tcgatcaccc ggaacaggca cagggccgga agccaataac
     3421 cgaacgggag tccccagatc gccacccagg tgcccacgac ctcatcggcg accacttcgg
     3481 gggggtcttc cctgccgacc tgccgggcgt agcggcccga agcccagaca gagaggccgg
     3541 caaacagcag gatgaaccag gccggcaccg ggtgaacaac cgccacgagg aacgccgcag
     3601 cggatccaac agttccgggc atccccgtca ggtaccccag ccctccgacc gttccgatct
     3661 ttccttccca ggtctccggc attctatacc ccattcccct gcacctccgc gaccagatcg
     3721 tgttccaggg cttccttgac ggtgacggtg acgaaacatc ccggctgcaa agcaccaccg
     3781 cctcgaatct cgacgaggcc gtccacttcc ggcgcttccc ggtaggacct cccaagggcg
     3841 agatcttctc caggctcgac cgattcaacc aggacctcga gggatcttcc ctcgaaccgc
     3901 ccctgccttt caaatgatat ttcctcctgg aggcgcatca gcctgtccat gcgccgcttc
     3961 ttcacttttc gtgggacctt gttccgaagg gacgcggaaa gggttccctc ttccggagaa
     4021 aactcaaacg caccgagacg gtcaaactga accgaggcaa taaaatcgag caaattcccg
     4081 aaagcggctt catcttcccc cgggtgcccc accatgaccg tcgttcgaag cgcaaaatcc
     4141 gggtccgccc cacggatctt cccgaaaatt tcccgcagtc gcccgggagg gatttcccga
     4201 ttcatgcttc tgaggacgcc ggcgtccgca tgctggatgg ggacatccag gtatctcagg
     4261 attcggctgc tgctcagcac ttgttcaagc aaggcgtcat cgatgcctga agggtgaaga
     4321 tagagaaggc gaagccatgt gccctccgga acggcgctct cgagttccct gagaagcaga
     4381 tgaagcgatc gcttcccgaa aagatccacg ccccatgccg tcaggtcctg ggccacgagg
     4441 cagatttccc ttgccccctg ccgtgcgagc tcgccggctt ccttcgccag aaagtctgga
     4501 gggaggctgc gcacccctcc gcgaatcgag ggaatcgtgc agtacgagca acggttatcg
     4561 cacccttctg caattttcag gtagcgggtc cagggtagcg tgccgggaag cgccttcctt
     4621 ccctcgcggc aatagggctc ggtttgcccg agtctctccc ccagtgtccc ccagtcttcg
     4681 gcttccgccc agaaatccac agaggaaagt tctttcctga gtgtttcgcc gtagcgattc
     4741 agaaggcagc cgaccacccc gatccgttct agcctcccgt tcgccttcag ttcctccatc
     4801 tgaaggatgg cacggatgct ctcctcggct gcgggacgga tgaaaccgca ggtattgacg
     4861 atagccaccc cggcttcttc caccgtttcc acgagttcgt acccccaggc gacaagggca
     4921 ttcgccaggc gttcgctgtc cacctggttc ttcgcgcagc cgaggctgag caaaaacagt
     4981 ttccttgccc tatcggaccg taaacgagcc atccggcttg tacaggaccg taacgacctt
     5041 ctgctgtgtc gaaggcctgc cgaggtcctt ccccatccat cgaacggcta cccccgtcgg
     5101 tttgccgtac cggacacgga tttccttttc cgacgggaag cttttgcttt ctcccttttt
     5161 cagcgtcccc cggaaaagcg tctcgccctc ccttgtgacc tggatccagc aggattccag
     5221 cgcggtgatc tcgagccgcc ccgatacgct ttcgccggag ctgcccgttc ccggcccatg
     5281 gcccggaagc cagctcaggt catcgctttg cggctgcaag ccagccggat cgcctgcgga
     5341 cgtctgcgtt tccagaggaa cggaggggcg atccagggaa gtctgcgtgg cgggcggtgt
     5401 ctgcaattgc gcggccactg tcgcttgctc ggctttcttc tcgatcgcct tcccaagaac
     5461 ccccttctgc tgccagagca ggtatcccgc caggagaacg gcaagggcca acaggacaac
     5521 ggcccaccgg ttgtccctgc gccggaagcc tctcgtaacc gcgggcttgg caaaggaccc
     5581 ctccggtggt tcggcacgaa cctgggattg gaaaaactcc tcatactggg gcaggagttc
     5641 gcttgagcca agatactcaa ggtagagccg gaccattccc cgggcgtaga cgggcgcggg
     5701 gagcgcctgg atctcccctt tttcgatggc cctcagaatc tcaacccgaa ggcgcgtttc
     5761 agcggaaaca tcctcgaggg ttttcccctt ttcctttcgt ttccagcaca ggtaagcacc
     5821 aagttccctc agcgtctgat cattcaccgg aacccgcctc cctcgccttt ttccgaagca
     5881 gcctggcggt caatgccgga tccgcctccc cgaagatggc attcccggca acaatcacgt
     5941 cacagccact cttaacgact tcctctacgt tggaaagccc gatccctccg tccatctcca
     6001 cgaggaattc gagcccctgt gcctctctaa gctgacaaag tgtacgggtc tttgcaagga
     6061 cttccgggat gaaggcctgg ccaccgaacc cggggttgac agacatgacc aggacaagat
     6121 caacaaaagg caggatcggc ttcagggcct ccacgcaggt ggcggggttc acggccacac
     6181 caggcctgct gcctccttcc cggatggcct ggagaacccg gtggagatgg ggggtcgcct
     6241 cctggtgaac caccaggtaa tccggtttcc agcggaggaa ggaatcaatg aaatcctccg
     6301 gcggctccac catcagatga acgtccagga cggcttcagg gagagccgat ctcaaggcct
     6361 tcagcagagc cggtccgtac gaaaggttgg ggacaaagtg cccgtccatg atgtcgaggt
     6421 gaatccagtc gtattcaccg gcgagcgact gaatgccttc ccagatccga aggggatcgg
     6481 ccgaaaggag ggacggagcg attttcgggg caattctctg tccgttcatg gacggtacct
     6541 gtcctgccag acgaattccc cgccgaggta cacggtcaca gccgcctccc ggacgtaggg
     6601 gacgttgacc gcgatcgatt ccccgccctt gacctccctt tcaaggacgg tccgcgtccc
     6661 ctcgggatcc accacttcga tcctcaggga gagcggcctg gaaagtggcg gcacctgata
     6721 gcgaaccttt gccgtgtgcc tcgtttcttc ttcctttgcc gaagcgggtg aagctgcggc
     6781 cgggacttcc tttttcggag gcacggcctg ttccgataca ggcttttgct cgggttgctc
     6841 ggcaaccttc ctcgcggggg cctcaggcgt tcccgcagag ttttcctgtt ttttctccac
     6901 ggttttgggt ttcggcgggg cttccgggtc ttcttcggcc ggaggagaag gagcctttgc
     6961 ctgggagaca ttcgacggcg ttgcaacggt caaggccaca cggcttccgg ccggcaccgc
     7021 ggctcccgtt ttcggggtgg tggaaaccac gatccccgga gcggtttcct gcgacaccag
     7081 gtagcggatt tcaaccccaa gccctgcggc ctcaagaagc gcccttgctg ccccctctgg
     7141 ctggccgacg acatccggaa ccgacaccgc actcccttcc acgccggtcc cgcggctcag
     7201 gagaagatcg atgcgggttt ccgggccgac catggccggt gcagccggac tctgtgccag
     7261 aacggatcct gccggtttcg ccggatcggc aacccggagg acgtctccca cctggaagcc
     7321 ggcttcggcc agtttggcct tggcttcgtt gaattccatt ccccgcacat ccgggagagg
     7381 ctttttctcc ccgccccggc tgaccttcag cgtcacgagc cgccctttgg ccactttttc
     7441 agaggccccc ggccactggg aaacgaccgt acctgccggg agctgggacg tcacctggtc
     7501 gacacggacc agcagcccgg ctttctgaag cgtttcgacc gcctccgagg aaatcatccc
     7561 ttccacggcc ggcatgggaa tcgttcggcc tcccatgaag acgaaataga aagctgccgc
     7621 cgccgagccg gcgatgacca gaacgagcag cagcatcgtc caacggatcc atttcactcc
     7681 agtatccccc ctaggctctt ttcataaagg ccgcgacgta aaaaccgtcg atccacggga
     7741 gtttcggcca gaggattcgg ccgtagggct tccccttgac cgagacggtt cccggaaggc
     7801 tcaaaggcaa ctccaccagg tctccgccaa agcccagaac cgaaccgacg acctgttcgt
     7861 tttcctcccg gaagaggctg caggtacagt acaccacgat tccgccaggt tgcacaagac
     7921 ggaacgccct ttccagcagg gcggcctgaa gcccggccat ctgcgcgagg cgttccggcg
     7981 aaagacgcca cttggactcc ggatgccgtt tccaggttcc gcttcctgaa cagggcgcgt
     8041 ccagaaagac cgcccttggg gcccgttccg gttccagcgt ccgcgcatcc ccgatcttga
     8101 acacggcacg ccgcgcgacc ccgattcggt gcatttccgc cttcgctgaa gccacttttc
     8161 ccggactcag ctcccaggat tccacgggga tcccttccgg caaacggcac agaagctggc
     8221 cgcttttgac cccccttccg gagcacatct caaggaccgg gccgccgtca agcagctcca
     8281 gaaaggcttc gacaaccagc atcgaggatt ccgtctgggg agtcacccac ccttctgcaa
     8341 aaccgggcag ggatggggga aacgggttgc tcgaaagcct gcagctttcc cgaagaagag
     8401 gggaaggcca gccccggatt ttggccctct ccagggctgt caaaaactcc tgttcctttt
     8461 cgggcctgtt caggcgaacc gagaggtagg gcttcatcga agaaagcttc cagaggttcc
     8521 gggcttcttc ctttccccac tcttcgatcc agcgacgtcc agcccacgag ggaatcccca
     8581 ggaaaagaca ctggtccttc gcatcagccg aactggccaa ttttgccaga acttgcggcg
     8641 cctcctcacg caccctcctg aggacagcgt tgaccaaccc cgcctcatgc ggaaaccggc
     8701 cggccttgac atgccccacc agcgcactca cgagcacttg cggggaaaaa tggcgaagtt
     8761 cgatcaagcc tgccgctccg accatcaaaa catctgcggt ttcagccgaa agcgcatgga
     8821 gcggcttcct caggaaaagg cccagcaggt gcttccacag cgattccctc ctggaagtcg
     8881 cataaaggag ggaagacgcc agcacccggt cagggggatc cagttgggct cccaccttcc
     8941 gcagcgcttc cgcggcaaag gctccctttc gaacttcccg gcgacattcc agagcagctt
     9001 cgatccctct catgaagacc ctgccttcca cggtgttccc ggacctttcg aatcatacca
     9061 ccgttgcctt cagcccgctc ccgcccaaga ggcgggcctt caaaaaaaag cgaaaggggg
     9121 tgagggtctc cccccactcc cctggatttc aactctgaag aacgggctaa tccctccgtc
     9181 ccccaaccat gccgcgcagg agaaggagac ggaaaagctg ggcgacggcc atgacagccg
     9241 cggcaacata agtcagagcc gcagcccgta gaaccgccct ggcacccccg atctcgtcca
     9301 tgtccagggt ccccgtgtcg gccagcaagc ggagagcccg ggcggaagcg tcaaattcca
     9361 ccggcagcgt cacgaggtgg aagacgatca cacccagaaa gaagaggatt cccaggtcca
     9421 tcaggaatgg agtccgcatc agcaggccga tgaagaacag aggaaacgcc agccaggagg
     9481 tcaggctggc aaccggaaca atcgcattcc gtacccggat cggcgcgtag tcctccgcat
     9541 cctggatcgc gtgtcccacc tcatgggcgg caatcccgat cgccgccagg ctggagctcg
     9601 agtaaacgcc ttccgacaac cgaagcacct ttgaccgggg atcgtagtga tccgtcagat
     9661 ttccagggac tcgctcgatc gcgattccgg aaaggccgaa ccggttcatg atcatcctcg
     9721 ccacgtcaca cgccctgact cccctgcgcg agggaacctg gctataggtc gcgaaggccc
     9781 cctgaacctt ggcctgggcc cacaagccga gcaagagcgc aggaatcagc agcacaaaag
     9841 tcggatcgaa catagggaac atgctccttt aacctcctct atggtgcggg tcaagctgct
     9901 accgcaacct tcgattcgga ttttacacga ctttccttca aaaggcaata ttggtcagat
     9961 atcaacggtg gtaaaactcc gccatcgcgg acaccagcca ttcctgttcc tctgccttta
    10021 gctccgggaa aatgggaagg gcgagacagt cacggctaag ggcctcggcc tccgggaaat
    10081 ccccctcggc atatcccaga cccgagaaac agggctgcag gtgtagacac agcgggtagt
    10141 aaaccctcgt gctgatgcct tccttttcca ggtaggcctg gagcgtatcg cgatcccggc
    10201 agcgaacgac atactggtgg tagatgtggg tgtttccaga ggcctccacc ggcggacgga
    10261 cctgctccag caggccgtat tcccggaaaa ggagcgcgta ccgggccgca gcctgccttc
    10321 gttcttcgtt ccattcctgg aggtggcgca accgaaccct caggatcgcc gcctggatct
    10381 cgtcaagccg gctgttgatc cccacgagct catggaagta ggtttccgtg gagccgtgga
    10441 cccgaagact tttcaggctc ctggccaggt cctctctcgt ggaggtgatc atgccggcat
    10501 ccccgtacgc cccgaggttc ttcgtcggaa agaacgagat acaccccagg tcgcccaccg
    10561 ctccggcacg gtagatcctg tcgcccacct gacggcaggc gccgaacgcc tgtgcggtgt
    10621 cctccaccag ggcaatcccc ttctcctgga gttcgggaag aagcctttcc accggaacca
    10681 tttgcccaaa aaggtgaacc gggatcaccg ctttggttct cggagtgatc ttctcaagaa
    10741 cctgggcgat gtccaggttg taggtttccg gatcgatatc cacgaagacc ggccttgcac
    10801 cttgccgggt gatgcagctc gcggtggcga aaaagctgta gggtgtcgtg atcacctcgt
    10861 cgccgggacc aaccttgagc gccatgaggg caaggacaag cgcatccgtt cctgaggcgc
    10921 acccgacggc atagggagct cccaccattc gcgcggcttc ttcctcgaaa gcacggactt
    10981 cggggccgag aatgaaatgc tggctctcaa ggaccttgtc cacggcctct cggatttctt
    11041 ctctaacgcg ggcgtaattg cgtttcagat ccagggtcgg caacctgcga tcgggcatga
    11101 aaacccctcc taaagaatgt gcgggaatcg aacaacggga ttatatcaac tcacgggtta
    11161 atccgggatt tttttgctct ttttttcgca taatcgtaac cccgaaaaaa ggggccccct
    11221 cgggggcccc tggctcgttc gttcagaatt cctccggttc cttgggcagc tggaggcctt
    11281 ccatggaaaa ccggagatgt tctctcatca gagcctccgc cttttctggc gatcgttctg
    11341 ccagcgcggc gatgatggca cggtgctgcg ggatgctcgg attggtgtcg atgtcgtaaa
    11401 aggggtcgta aaaaacaata tagacgttcg tccgggccag gatgttttcg acgtactcag
    11461 ccaggatccg gttcccgctt gactcggcga tggtgcggtg gaagttctcg ttcacttcca
    11521 ggtatagttt caggtccctt tcatggaagg cccgctcttc ttcgatgacc gaatcttcca
    11581 gccttcgcag ctggcgttcc gtgattcgct cggctgcaag gcgaatcgcc aggcactcaa
    11641 ggtgctctct caggacatag gcgttttcca tttccttttg gctagggcat gcaagacggg
    11701 ccccgctgtt gggtatgatc gtgaccagtc cctcgctggc gagccgccga agggcttccc
    11761 ggacgggcgt ccggctgact cccatctgaa ccgcgatgtt cacctcgggc aggcgctgcc
    11821 ccggctgtag ctgcttggtg atgatgcggt gcctcaggtc ctggtagaca tagtcggcgg
    11881 atgtcgtgta gaagcgcgcc tctctcatcg atccacaaac ctcccgtcat cctgcatacc
    11941 gttcc
//
LOCUS       DAOOVN010000030        10739 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_3954_length_10739_cov_15.165575, whole genome shotgun
            sequence.
ACCESSION   DAOOVN010000030 DAOOVN010000000
VERSION     DAOOVN010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 10739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10739
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3954_length_10739_cov_15.165575"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="PLY83_04135"
     CDS             complement(<1..578)
                     /locus_tag="PLY83_04135"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOV29262.1"
                     /translation="MNKLNIGTNPFLLPMPMVIVGTCIGERPNFMAVGWASRVNVKPA
                     IMSVAIGRHATAEGILESGEYSINIPSVDLIRHTDLVGMVSGAEFDKSGLFDVFYGSL
                     GKAPLIRQCPVNIECKVIQVVELPMDRLFIGEVKRVWSEERYLTEGSPDIEKVRPFCL
                     SMPDNRYWSVGAVVGKAWQDGLDLKGRLSRIEG"
     gene            834..2165
                     /locus_tag="PLY83_04140"
     CDS             834..2165
                     /locus_tag="PLY83_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOV29263.1"
                     /translation="MKTGINVHYRYTALLAAIVFGVTVFPVKLCASTPAATLPPVSPV
                     VLKKMDTEWIKEDGEKATPMIRPPTHTLGPSVDYSSLVTLTGNQDGWGGCIGRSIIHV
                     IDILNERKHPYAPDLSFWYFHRRQEMAGIDGYSLVQKYGLCSESDLPTNYDKAQCHFS
                     GYDPNGIPQYTWDWSDLPQPTPRIDALAANHRVWVGLPQVPDVEDMKYCLETDGPILA
                     SGPLVAIQGEAPAEWHSVAVVGYSDDRKSFRCLNSWGDTWNGDGYFEIPYEKVNANFM
                     AFRRVVLYDSKPEPLTMRIWIDGMQDGRRKIIVRVGMEDVGDVPVWNAPNQFLCVDDS
                     RYLKIDVPVPTLRAVQPNKRWYVEITNTGAGFIRVLELTLARRSKDQFGNPVTKLQTY
                     NGQEDFWIGFDSKASAQPATKRFYFPLEEKLQIAPQRLFQMKPQRIIKTQP"
     gene            complement(2352..>2781)
                     /locus_tag="PLY83_04145"
     CDS             complement(2352..>2781)
                     /locus_tag="PLY83_04145"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOV29264.1"
                     /translation="EGPGRVVQGKARGHELAVDLLSSWGGGDSHACPLETLAFALGAC
                     FVATARTWAIREKLPIASISADVQGTVDLTRGMGKQGKNRLGFPFLKISVKLDSPLPE
                     ETQQAFLEKVASLCPVCDTIERATPIQIELLPKNQQEGLS"
     assembly_gap    2782..2881
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2882..3808
                     /locus_tag="PLY83_04150"
     CDS             <2882..3808
                     /locus_tag="PLY83_04150"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HOV29265.1"
                     /translation="VLGAGAMGSIIGAALSKGGQEVLLVDPYREHLDRIAGKGLRLTF
                     GSVTETVRMQTCTNPAEAGQADVVVLLVKGFMSKTAIEGARGLFGKNTFVCTLQNGLG
                     NVEILEGLFPRDRILQGVIHLTGRMVGPGEVAGDKYRETDLYLGSLAKEGSAVMVAET
                     MARHLSDGGLLAEYMPDVEKEIWRKAVINACLNAPCGVLRLRGREYFGHPEGKRIAAD
                     IAAEFAAVAAAKGIVIDVDEAMRYMEMGTKTRSEHYPSMAQDMMNRRRTEIDFLNGAI
                     VKYGQELGIPTPVNDTITRFVKIIEENYDVQF"
     gene            4256..5596
                     /locus_tag="PLY83_04155"
     CDS             4256..5596
                     /locus_tag="PLY83_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOV29266.1"
                     /translation="MGVFHEYEQKLKSPEEAVGVVKSGDWLDFGFGLSMPDLLDEALS
                     HRVPDLTDLKIRGALALRPLKTVEADPGRESVCYSSWHFSGYERRLHDAGLCNYIPMI
                     YRNKPNFYRKSLEVDVAMLQVAPMDKHGWFNFSLTNSASRAICETARYVILEVNEALP
                     RALGGREECIHISEVDAVVEAGNRPPVEMRPAEPSDLDRQIAAHIVEEIPDGATIQLG
                     IGGLPNAVGSLVADSGLRDLGMHTEMLVDAYLVLNRKGKLTNRRKGIDRLKGTWTFCV
                     GTRDLYEWVNENPGLASYPVDYTNDPRVMAQLDSLISINSCIEADLYGQVCSECSGIR
                     HISGTGGQLDFVTGAYLSRGGKSFICFPSTYTDKTGQIVSRILPVMREGSVVTDPRTQ
                     THILVTEWGKADLAGRSTWEKAERIIGISHPRFRDHLIRCAEEMGIWRKTNRIP"
     gene            5639..>6047
                     /locus_tag="PLY83_04160"
     CDS             5639..>6047
                     /locus_tag="PLY83_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29267.1"
                     /translation="MIKRKRSSSGFARMPREAGGQEPIGLISRRAWGELDFERARQRH
                     LLLGGNAPPSGIFPSTDFLPFFAFLVHSPAAFLIVEELHRDLQGKTRKTRNCEAIWYA
                     SAEPSKALLVERVLPLRKRGLIEISNGNVVRYEA"
     assembly_gap    6048..6147
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6302..7048
                     /locus_tag="PLY83_04165"
     CDS             6302..7048
                     /locus_tag="PLY83_04165"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOV29268.1"
                     /translation="MDIPRIFTITESAHRIHNPFTPEKLATLGAALRLEEGMRVLDLG
                     SGSGEMLCTWARDYGIVGTGIDLSQLFTEQAKRRAEELGVADRVKFIHGDAAGYVSDE
                     KVDVAACVGATWIGGGVAGTIELLARSLRPGGIILVGEPFWRQLPATEDIARECDAES
                     ISDFFMLPELLASFGSLGYDVVEMVLADQDSWDRYEAAQWLTMRRWLDANPDDEMVQE
                     VRARLTSEPVRYAAFRREYLGWGVFALMAR"
     gene            complement(7175..7741)
                     /gene="rfbC"
                     /locus_tag="PLY83_04170"
     CDS             complement(7175..7741)
                     /gene="rfbC"
                     /locus_tag="PLY83_04170"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOV29269.1"
                     /translation="MKATELELKGLWLFQPEVFPDERGSFMETWNARKYLEFGVPSSF
                     VQDNLSRSSKGTLRGLHFQNPRPQGKLVCVLKGQVWDVSVDLRLGSPTFGKWTAVTLD
                     DKLGNQLYIPPGFAHGFCTLSEEALFFYKCTDYYSPRDEHGIAWNDPCLAIDWPIREP
                     ILSLKDQGHPQLRGISSRVLFLMPSSHD"
     gene            complement(7722..8618)
                     /gene="rfbA"
                     /locus_tag="PLY83_04175"
     CDS             complement(7722..8618)
                     /gene="rfbA"
                     /locus_tag="PLY83_04175"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOV29270.1"
                     /translation="MTGKGIILAGGSGTRLYPTTISTSKQLLPIYDKPMIYYPLSVLM
                     LAGIRDILVITNTDHRKAYQQLLGDGSQWGISVSYGVQPEPGGLPEAFLVGKDFIAED
                     PCALILGDNFLYGQGLTGLLRRAALRESGATIFAYPMKDSRPYGVVEFDSEGRACRIE
                     GKPEKPRSNFAIVGLYFYDGTVVEKAESLRPSRRGELEISELNQLYLEEGTLSVERLG
                     RGFAWLDAGTPDTLLEASKFVSTIQNRQGLMISCPEEIAWRRGFIGKERMIELAGQLK
                     GGPYRDYLLSLLEGSQNESNRT"
     gene            complement(8623..9684)
                     /gene="rfbB"
                     /locus_tag="PLY83_04180"
     CDS             complement(8623..9684)
                     /gene="rfbB"
                     /locus_tag="PLY83_04180"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOV29271.1"
                     /translation="MGRKDKSLLVTGGCGFIGSSLVRLALDRGYEVLNLDKLTYAGNL
                     VSMVEVRSASYSFLQADVSDLALVSEAFGRFKPSGVIHLAAESHVDRSIDEPASFVQT
                     NIVGTYSLLQAALNYWTRLDATCRKAFRFLHVSTDEVFGSLGPEGKFQESSRYDPRSP
                     YAASKAASDHMVRSYWHTYGLPILVTNCSNNYGPRQFPEKLIPLTITRAIRGKSIPVY
                     GDGRNVRDWIHVEDHCEALLQVLDKGRTGETYLIGGHAEKSNLEVIQETCALLDQLAP
                     RPNGLYHYDLVEFVPDRPGHDWRYAMDTSKIETELGWHPKKEFHLGLRQTVEWYLSNQ
                     AWVDQVLLPRERNGQMKRG"
     gene            complement(9668..10654)
                     /locus_tag="PLY83_04185"
     CDS             complement(9668..10654)
                     /locus_tag="PLY83_04185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOV29272.1"
                     /translation="MNELASIIVFTFNQENLVEDTLNSTLHQGYRPMELVICDDASED
                     NTGTVCKRWLESRQDAFERVLYCRNETNLGTTRNIMKGISLSTGNLIKLLGGDDLLAP
                     DAIKNGMRFHLESPESVIFGNVIGFQWNGKDFLVKETEPGLHTRRLLSLPPRRQFGIL
                     SICSSIPAPGAFYPRSFFDRISSGLALTTHIPDWPRWLEATSLGISLLYRDFDAVYYR
                     RHAHAHKNSGYRAKCILDVLHCIDNQIKKNEDLLSWLQRVGVWANRQKHEKLLQGRDF
                     EAAFFNSILNLVRHAVRFNLYFPLAHERPATIPGTPLKLPFELRRPSLGQER"
BASE COUNT         2363 a         2804 c         3110 g         2262 t
ORIGIN      
        1 ccctcgatcc tggacagcct tcccttcaga tctagcccat cctgccaagc ctttccgacc
       61 acggctccca cggaccagta gcggttgtcg ggcatcgaca ggcagaaggg gcgaaccttt
      121 tcaatgtcgg gactgccttc ggtcaggtag cgttcttccg accagacccg tttcacctcc
      181 ccgatgaaaa gccggtccat gggaagctcg acgacctgga tcaccttgca ttcgatgttg
      241 accgggcatt gccggatcag aggggccttc ccaagggagc cgtaaaagac atcgaaaagc
      301 cctgacttgt cgaattcagc tccgctgacc attcccacga ggtctgtatg ccggatcaag
      361 tcgacggagg ggatgttgat gctgtactcc cctgactcga gaatcccttc cgccgttgcg
      421 tgccggccga tggcgacgct catgatcgcg ggtttcacat tgacgcggct agcccagcct
      481 accgccatga agttgggtct ttccccgatg cacgtgccga caatgaccat tggcatgggc
      541 aaaaggaagg ggttggtgcc gatattgagc ttattcattc cggcaagcct cctcttctgg
      601 tttggaatat ccaccaaaca ttctaagcct ttccggcagg ggacggtctg tcgccctgaa
      661 gtcaagccaa aaggtgaggt tggagaaatt tttcttggta ttctacacta aactatcaga
      721 gttataatga ataactaaat gagacaagcg ataccatttt cgacagcccc gttttcctca
      781 ctcgccaaga gaaacgaagg ccagcgtgtt ttttgaagaa cggagggatc aagatgaaga
      841 cgggaatcaa cgtgcactat cgctacaccg ctcttttagc ggctattgtt ttcggagtca
      901 ccgtctttcc tgtgaagctt tgtgcgtcga cccctgctgc gactttgccc ccggtttctc
      961 ctgtcgtcct gaaaaaaatg gatacggagt ggattaagga ggacggggaa aaggcaacac
     1021 cgatgatccg gccgccaacc cacaccttgg ggccgagcgt ggattattcc tccctcgtta
     1081 cgttgacggg aaaccaggat ggctggggcg gatgcatcgg ccgctccatc attcacgtga
     1141 tcgatatcct gaatgaacgg aagcatccct atgccccgga cctctctttt tggtattttc
     1201 accgtcgcca ggaaatggcc ggcatcgacg ggtattcgct ggtgcaaaaa tacgggttgt
     1261 gttcagaaag tgatcttccg acgaactatg acaaggcaca atgccacttc agcgggtatg
     1321 accccaatgg gattccccag tatacctggg attggagcga tttgccgcag cccacgccaa
     1381 gaatcgatgc cctggctgcc aaccacaggg tatgggtcgg cctgccccag gtccctgacg
     1441 tggaggacat gaagtactgc cttgaaacgg atggccccat cctggcttcg gggcccctcg
     1501 ttgccataca gggagaagcc cccgcggaat ggcacagtgt cgctgttgtc ggctacagtg
     1561 acgacagaaa gagtttccgg tgcctgaaca gttgggggga tacctggaac ggggatgggt
     1621 attttgaaat cccgtacgaa aaagtgaatg ccaatttcat ggcttttcgg agggtggttc
     1681 tctacgattc aaagcctgaa cccctgacga tgaggatctg gatcgacggg atgcaggacg
     1741 gccggaggaa gatcattgtc agggtcggga tggaggatgt cggggatgtc ccggtatgga
     1801 atgccccgaa ccagttcctt tgtgtcgatg acagccgcta cctgaaaatc gatgttccgg
     1861 tgccgacctt gcgagcggtt cagcccaaca agcgttggta cgtcgaaatt acgaacaccg
     1921 gagccgggtt tatccgcgtc ctggaattga ccctggcccg acgcagcaag gatcagtttg
     1981 gaaatcccgt gacgaagctg cagacgtaca acgggcaaga ggatttttgg attgggtttg
     2041 actcgaaagc cagcgcccag ccagcgacca agcgatttta cttccccctg gaggaaaagc
     2101 tccagattgc cccgcaaagg ttgttccaga tgaaaccgca acgaatcatc aagactcaac
     2161 cctaaacgag gctttccgga tcggcctgtc cgattaaacg ggtcaatagt gatctcgtgt
     2221 tcggcacgaa agggatggcc agtttccggt tgacttcgag gaaatcctcg ttaacctttc
     2281 gtggccctct tttgtggcaa taaagaccga acattcgcag caaagaagtc cgcaagttgc
     2341 ggaaattcct ctcaggagag tccctcctgc tggttttttg gcagcaactc gatttggatt
     2401 ggcgtcgctc tttcaattgt gtcgcaaacc gggcaaaggg aggcgacctt ctcaaggaac
     2461 gcctgctggg tctcttcggg caggggagag tctagcttga ctgaaatctt gaggaacgga
     2521 aaacctaggc ggtttttccc ttgctttccc atccccctgg tgaggtccac ggttccctgg
     2581 acatcggcgg aaatggaggc gataggcagt ttttcccgta tcgcccacgt tctggcagtc
     2641 gcgacgaagc aggcccccaa ggcaaaggca agggtttcta gcgggcaggc gtgcgaatcg
     2701 cctccgcccc aggaagagag aagatcaacc gcgagttcat gtccgcgggc cttgccctgg
     2761 acaactcttc cggggccttc annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 ngtgcttggc gcaggagcga tgggaagcat catcggggcg gccctgtcca agggaggcca
     2941 ggaagtcctt cttgttgatc cataccggga gcatctggac cggattgccg ggaaagggct
     3001 caggcttacc ttcggttcgg ttaccgaaac cgtgcggatg caaacctgca cgaacccggc
     3061 cgaagccggg caagccgatg tggtcgtcct gctggtaaaa ggtttcatgt cgaaaaccgc
     3121 gatcgagggc gcgcggggat tgttcggcaa aaacacgttt gtctgcacgc tgcaaaacgg
     3181 gctgggcaat gtcgagatcc tcgagggact gttcccgcga gacaggattc tgcagggggt
     3241 aatccatcta accggtcgca tggtcggccc gggggaagtt gcgggagata agtaccggga
     3301 aaccgacctc tacctgggca gcctcgcgaa ggaaggttcc gctgtgatgg tcgccgaaac
     3361 aatggccagg catctctccg acgggggatt gctagcggag tacatgccgg atgtggagaa
     3421 ggagatctgg agaaaggcgg tgatcaatgc ctgcctgaat gccccctgcg gagtgctgcg
     3481 ccttaggggc agggaatatt tcggtcaccc tgaggggaag cggatcgccg cagatattgc
     3541 agcggaattc gccgcggttg ccgccgcgaa ggggattgtc atcgatgtcg atgaggccat
     3601 gcgctacatg gaaatgggca ccaagacccg aagcgagcat tatccctcga tggcccagga
     3661 catgatgaac aggagaagga cggagatcga ttttctgaac ggggccatcg tgaaatacgg
     3721 acaggagctg gggattccca cccccgtgaa cgataccatc acgaggttcg tgaaaattat
     3781 cgaagaaaac tacgacgttc agttctgacg agattgcggg ttcgagccca gtcacgggtt
     3841 ggcccgcggc aagaatgaag aaagcatcgc ggagaaggct gctggcaaat gtgctatcgg
     3901 tcctcttcgg gcctttcctt ctgcataata tcggcaaggc aggattgaaa ccgctaactg
     3961 agcccgaaac ggttacgttt gccccggcgc tcgtcctccg gacggttgcc cagaacacac
     4021 gatgcacggc cgcaacgttt ccttcatgcc gcagcttccc cattcttccg gcggatcggt
     4081 gcaggttttc ccgtgtcttg agttcaacga agtttccctt tttcgacccg gcggctcgta
     4141 gctgcgagtc tcgtcggtgc gggaaggcga actcgtccga cagaaaaaaa gcgttcctat
     4201 ggatattttt gtttataatt gaccctacta ttgattcaat aggggggtgc cgagaatggg
     4261 agtgtttcac gagtatgagc agaaactgaa aagcccggag gaagcagtag gagtggtgaa
     4321 gagcggtgac tggctggatt tcggttttgg cctttccatg cccgacctcc tggatgaagc
     4381 cctttctcac cgtgttcctg atttgacgga cttgaaaatc aggggggcgt tggccctgag
     4441 gcccctgaaa acggtggaag ccgaccccgg acgagagagc gtgtgttatt ccagttggca
     4501 ttttagcggc tacgagagaa ggcttcacga tgcggggctc tgcaactaca ttcccatgat
     4561 ataccgcaac aagccgaatt tttaccgtaa aagcctcgaa gtggatgttg ccatgctgca
     4621 ggttgcgccg atggacaagc atggatggtt caatttctca ttgacgaact cggcttcaag
     4681 ggccatctgc gaaacggcac gatacgtcat cctggaagtg aacgaggcgc tcccgagggc
     4741 acttgggggg cgagaggaat gcatccacat ctcggaagtg gatgctgtcg ttgaagcagg
     4801 gaatcgaccc cccgtagaaa tgcgccccgc ggaacccagt gacctggacc gccagatcgc
     4861 agcgcatatc gtggaagaaa tccccgacgg agcgacaatc cagcttggaa tcggcggact
     4921 ccccaacgct gtcggaagcc ttgtggcgga ttccggcctc cgggacctgg ggatgcatac
     4981 cgaaatgctg gttgatgcct acctggtcct gaatcgcaag gggaaactga cgaaccggag
     5041 aaaggggatc gaccgtttaa agggaacctg gaccttctgc gtcggtaccc gggaccttta
     5101 cgaatgggtg aacgagaacc ccggcctggc gtcttatccc gtcgactata caaatgaccc
     5161 gcgcgtcatg gcccagctgg attccctcat cagcatcaac agctgcatcg aagccgacct
     5221 ttacggccag gtatgttccg agtgctccgg cattcgccat atcagcggaa cgggaggtca
     5281 gctggacttt gtcaccggag cctacctatc gcgaggcggg aagagcttta tctgtttccc
     5341 atcgacctat accgacaaga caggccagat cgtctcccgg atcctgccgg tcatgagaga
     5401 agggagcgta gtcacggatc cccgcacaca gacgcacatc cttgtcacgg agtgggggaa
     5461 ggcggatctt gcaggccgat ccacatggga gaaggcggaa aggatcatcg ggatcagcca
     5521 ccctcgtttt cgggatcacc tgatccggtg tgccgaggag atgggaattt ggaggaagac
     5581 gaacaggatt ccgtagggag acacaggcgt agattccccg caggtgaaaa tcgatcgctt
     5641 gatcaagcgg aagaggtcct ctagcggatt tgccaggatg ccaagagagg cgggagggca
     5701 ggaacccatc ggcctgatct cccgccgggc ttggggcgaa ctcgattttg aaagagccag
     5761 acaacgccac ctgctgctag gagggaatgc tcccccctct gggatttttc cctcgacgga
     5821 ttttctgccc tttttcgcct ttttggtcca ttcccctgcg gcgtttctca tcgttgagga
     5881 gttacatcgg gatcttcaag gaaagacacg gaaaacgaga aattgtgaag caatctggta
     5941 tgcttcagca gagcctagca aagctctgct ggttgagcgg gtgttgcccc tcaggaaacg
     6001 cggattaatc gaaatctcca acggcaacgt cgttcgatac gaagcagnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnagg aaacgcggat taatcgaaat ctccaacggc
     6181 aacgtcgttc gatacgaagc agaaagcagt agccccggtg ccggaaaacc agtccaggaa
     6241 agcctggatt cccggttcgt cgggatcgaa gagattcaca agctgaagga ggttttctga
     6301 aatggacatt ccgcggattt tcaccattac cgaaagcgct catcgcatcc ataacccctt
     6361 cactccggaa aagcttgcca ccctcggggc ggcattgcgc cttgaagaag ggatgcgagt
     6421 gctggacctc ggcagcggtt caggggagat gctctgcact tgggcgcgcg attacgggat
     6481 cgtcggcacc gggatcgact tgagccagtt gtttaccgaa caagcaaaac gcagggccga
     6541 agaactgggc gtcgccgatc gagtcaagtt catccatggc gatgcggctg gttatgtctc
     6601 ggatgagaaa gtcgatgtgg cagcctgtgt cggtgccacc tggatcggag ggggtgtcgc
     6661 cggcacgatc gagcttctag cgagaagcct gcgtcccggc ggaatcatcc tcgttggcga
     6721 gcccttctgg cggcagttgc cggcaacgga agacattgcg agggaatgcg atgccgaatc
     6781 gatctctgac tttttcatgc ttcccgaact cctcgcgtct ttcggaagcc ttggctacga
     6841 tgtcgttgaa atggtcctgg ccgaccagga tagctgggat cgatacgagg cggcccaatg
     6901 gctcaccatg cgccggtggc ttgatgccaa tccggacgac gagatggtcc aggaagttcg
     6961 agcccggttg acctcggagc ccgtgcgata cgccgctttt cggcgtgaat acctgggctg
     7021 gggcgtgttc gcgctgatgg cgcggtaatg cgttggggct ctcgtgaaag ggatcccgga
     7081 aaaaggatca gccttggcgg ggttcaccgg agaagatccg gatcggaaaa ccaaaggtgg
     7141 tgagccgatc cacgcaggaa gtattccggg acggttagtc gtgcgaggac ggcatcagga
     7201 aaaggacccg tgacgagatg cccctgagct gaggatgtcc ttgatccttt aaagacagga
     7261 tcggttcgcg gatcggccaa tcgatcgcga ggcaagggtc gttccaggcg ataccgtgct
     7321 catcccgggg agaataataa tcggtgcact tgtagaaaaa cagggcttcc tctgagagcg
     7381 tgcagaagcc gtgagcaaac cccggtggga tatagagctg gtttcccagc ttgtcgtcta
     7441 gcgttacggc tgtccacttg ccgaatgtcg gcgagccgag acggagatcg accgagacgt
     7501 cccaaacctg tcctttcagg acgcagacaa gttttccctg gggacgggga ttctggaaat
     7561 ggagccctcg cagggttccc ttcgaggacc tggaaaggtt gtcttgcacg aaggacgaag
     7621 gaacgccgaa ttcaaggtat ttcctggcgt tccaggtctc catgaaagag ccccgctcgt
     7681 ccgggaaaac ctccggttga aaaagccaaa gtccctttag ctcaagttcg gttgctttca
     7741 ttttgtgatc cctccagcag gcttaagaga tagtcccggt acgggccccc ttttagctga
     7801 cccgcaagct cgatcatccg ttccttcccg atgaaccccc gcctccaggc aatttcctcg
     7861 ggacaggaga tcatgagccc ttgccggttt tggatcgtgg aaacgaactt cgaggcttcc
     7921 agcagggtgt cgggcgtccc tgcgtccagc caggcgaagc cccgccccaa ccgttccacg
     7981 gataaggttc cctcctcgag gtaaagctgg ttcaattccg agatctcgag ttcgccacgt
     8041 cttgagggac ggaggctttc tgctttttcg acaaccgttc catcgtaaaa gtaaagcccc
     8101 acaattgcga agttggaacg tggcttttca ggttttccct cgatacggca ggcgcgcccc
     8161 tccgaatcga actccactac cccgtaaggc cttgagtctt tcattgggta tgcaaaaatc
     8221 gtcgccccgg attccctcag cgccgctcgc ctgaggaggc cggtaagccc ctgcccgtag
     8281 aggaaattat ctcccaggat gagagcgcag gggtcttctg cgatgaaatc cttgccgaca
     8341 aggaaagcct cgggtagccc gccgggttca ggctggacac cgtaggagac ggaaatgccc
     8401 cactgcgagc cgtccccaag gagttgctgg taggccttcc ggtggtccgt gttggtgatg
     8461 accaggatat cccgaattcc cgctagcatg agcaccgata aggggtaata gatcatcggc
     8521 ttgtcataga tgggaagaag ttgcttgctt gtcgatatgg tcgttgggta gagacgggtt
     8581 cccgacccac ctgcaaggat gatgccttta ccggtcatga aatcaacccc ttttcatttg
     8641 gccgttccgc tcccgaggaa ggaggacctg atccacccag gcctggtttg aaagatacca
     8701 ctccacggtt tgccgcaggc cgaggtggaa ttctttcttc gggtgccacc ccagttcagt
     8761 ttcaattttc gaggtgtcca tcgcataccg ccaatcgtgg cccgggcggt ccggtacaaa
     8821 ctcaaccaga tcatagtggt aaagaccgtt tgggcgtggg gccaattggt ccaggagcgc
     8881 gcaggtttcc tggatgacct ccaggtttga tttttccgcg tgcccgccga tcaggtaggt
     8941 ttctcccgtc cttcctttgt ccaggacctg tagaagagcc tcgcagtggt cctcgacgtg
     9001 gatccagtcg cggacatttc ggccgtctcc gtagacgggg atcgatttcc cccggatcgc
     9061 ccgggtaatg gtcagcggga tgagtttttc cggaaattgg cgggggccgt aattgttgct
     9121 gcaatttgtg accagaatgg gcaacccgta ggtatgccag taggagcgga ccatgtggtc
     9181 tgaagcggct ttggatgccg catagggtga acgggggtcg taacgggacg attcctggaa
     9241 cttcccctcg ggtcccaggg acccgaaaac ctcgtccgtg gagacatgga ggaagcgaaa
     9301 ggccttccgg caggttgcat caagccttgt ccaatagttc agggcggcct gaagaaggga
     9361 ataggttccg acgatattgg tttgaacgaa ggaggcaggt tcgtcaatgg atcggtcgac
     9421 gtgggattcg gccgccaggt gaatcactcc ggacggcttg aaccgcccga acgcttccga
     9481 cacgagtgca aggtcactga cgtccgcctg gagaaaggag taggaagcag agcggacttc
     9541 caccattgaa acaaggtttc cggcataggt cagcttgtca aggttaagaa cctcatatcc
     9601 cctgtcaagc gccagtcgaa ccagggaact gccgatgaaa ccgcatccgc ccgtgacgag
     9661 aagagattta tctttcctgc ccaagggaag gcctcctcag ttcgaaggga agcttgagag
     9721 gtgtcccggg gatcgttgca ggcctttcgt gcgcaagtgg gaaatatagg ttgaaacgga
     9781 ccgcatgcct gactaggttg agaatactgt tgaaaaaagc ggcctcgaaa tccctgcctt
     9841 gcaaaagttt ctcgtgtttt tgccgatttg cccacacccc aactctttga agccatgaaa
     9901 gcaagtcctc gttctttttg atctggttat cgatgcaatg caggacgtca agaatacatt
     9961 ttgcacggta gcccgaattc ttgtgcgcat gcgcgtgtct gcggtaatac accgcatcga
    10021 agtcccggta aaggagggaa attcctaggc tagtcgcttc aagccaccgc ggccaatcgg
    10081 ggatatgcgt cgtcagtgcc agaccactgg aaatccggtc aaaaaaggac cgaggataga
    10141 aggctcccgg ggcagggatg ctgctgcaaa tcgataggat gccgaattgc ctgcggggag
    10201 gaagagagag gagcctgcgc gtgtgaagcc ccggttctgt ttccttgacc aggaagtctt
    10261 tcccattcca ctgaaacccg atcacgttcc cgaagatcac cgattcaggg gattccaggt
    10321 gaaagcgcat tccgtttttg atcgcatcgg gcgccaggag gtcatctcct cccagcagct
    10381 tgatgaggtt cccagttgag agggaaattc ccttcatgat gttccgcgtg gttccaaggt
    10441 tggtttcatt ccggcaatag agcacccgtt cgaaggcatc ttgccttgac tccagccagc
    10501 gcttgcaaac cgtacctgta ttgtcctcag atgcgtcatc gcaaatgaca agttccatcg
    10561 ggcgataccc ctggtgaagg gtcgagttca gggtgtcttc gaccaaattt tcctggttga
    10621 aagtgaaaac aatgatggaa gccaattcat tcaccgcgag caacctcccc gggcctctct
    10681 tttcgagtgc tttggatcga gagagagtaa actaatcatg agaatatcga aaaggggga
//
LOCUS       DAOOVN010000031         9797 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_4385_length_9797_cov_14.257134, whole genome shotgun sequence.
ACCESSION   DAOOVN010000031 DAOOVN010000000
VERSION     DAOOVN010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 9797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9797
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4385_length_9797_cov_14.257134"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PLY83_04190"
     CDS             <1..452
                     /locus_tag="PLY83_04190"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HOV29273.1"
                     /translation="MRPNALVGFAAAILGGLYTIQVHRLPKAIAGDASSPVSFPLALG
                     LLMTVLGILLVLQEARHGLTNLEGVKRPAFTAPSLRTIFRTVAASLLYAFLFERLGFF
                     VSTLGFLGTLLYLINGRRAWKLNAALALGFTLGAWALFVQLFELPLP"
     gene            466..>1874
                     /locus_tag="PLY83_04195"
     CDS             466..>1874
                     /locus_tag="PLY83_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOV29274.1"
                     /translation="MEFGSLLLKGFSVALTPVNLAWLLGGSILGTLFGMIPGFGPSTG
                     IALLLPLCLGLRPETALIAMAAIYYGAMFGGSRSSILMNVPGDSAAVASCFDGYPMAR
                     NGQAEAALAVSAVASLMGGLFASVAFTLLALPAARIALRFGPPETFCLMVFSLAATAA
                     VSRENLLKGLISLVLGLMISTVGLDSPSGVSRFTFGLEQLQTGFSFVVVILGIYGFSE
                     VLINLETLALETARPLQLEFRWIQISMAHWRRSIGPILRQSPVGFLIGILPGAGAGIA
                     ALRAYRNEKKRSPHPEEFGKGAVEGLAAPEAANNACSIGAMLPMMTLGVPGSGTAAVM
                     LGGLLLCGLQPGPLLFEPQPGIGWAVIASLFLGNFACAVINLPLAGFLVRILSIPPKA
                     FYPFVVAVSFLGLYTVNFSVTDLYLLVGMGICGYLMKKFRIPTGPLVLASVVGSSVES
                     SFRQSLELSGGSPSIFFRSS"
     assembly_gap    1875..1974
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2000..3565)
                     /gene="citF"
                     /locus_tag="PLY83_04200"
     CDS             complement(2000..3565)
                     /gene="citF"
                     /locus_tag="PLY83_04200"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HOV29275.1"
                     /translation="MNFVKNALGRLVPVGVDGVGAFSPYEGAFARVLKGAPWPAAYPV
                     RKPEPPSRSKVAPSLKTAIERSGLENGMTISFHHHLRNGDKVIPLVLETLLQMGFRDL
                     TFAPSSIPDSHDCVADYVRKGLIRRIWTSGARGEVGRMISRGEMEETVIIRSHGGRAR
                     AIEEGSIVIDVAFLAAPACDALGNSAGCSGKSACGSLGYAITDARFARHVIAVTDNLV
                     EYPLHPHISVPQHLVNQVVVVDSLGDPAKIASGAARITRNPLDLKVAENAYRLLAASG
                     ILAPGCSFQVGAGGASLAVAAFVRDHLKEKGIQGGFGLGGISGYMAQMLEEGLFRALF
                     DVQSFDSAVTGSMVKNRAHMEIDPSWYANPMNQGCLVHNLDVVILAALEVDTDFNVNV
                     MTGNDGVLRGASGGHSDTAAGAKMAIVVAPSFRGGVPTIKDRVQTLVTPGETVDAIVT
                     ERGICINPNRADLWEAAKKANLPLKEITDLKKEVERFTGVPEPVEMDRDRVVAVVEYR
                     DGTLLDSIYRVKE"
     gene            complement(3562..4449)
                     /locus_tag="PLY83_04205"
     CDS             complement(3562..4449)
                     /locus_tag="PLY83_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOV29276.1"
                     /translation="MRGMRSMLYIPGNAPGMIQNAAVFGADMVMIDLEDAVALSEKDA
                     ARDITAGFLRHYDFGDLPVVVRINGADTPFFEEDLEAIVPLKPFAIRLPKCQDPSDVL
                     LADSRMTEIEKRHGMSGGLVPIHAMLESARGIENAYAIATASSRVTALTLGGQDLTAD
                     MGIQKTRDGWELFYARSRVVMAAKAAGIAAFDTVWTDFNDPEGLFREARQIVELGFTG
                     KAAIHPSQVEVIHRAFRPDAKEVRKAERIVAAAAEAERQGRGVVAVDGKMVDAPIIVR
                     ARHLISLAGLYGMEGGEAQ"
     gene            complement(4454..4726)
                     /gene="citD"
                     /locus_tag="PLY83_04210"
     CDS             complement(4454..4726)
                     /gene="citD"
                     /locus_tag="PLY83_04210"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HOV29277.1"
                     /translation="MVELVVRMAQAGTLESADCLVTVVPAEEFRLDYRGVNRKLFGER
                     TRKLVMETLERTGFSGMQVTIQDQGALELVIRARLETALRRAVGEV"
     gene            4833..5543
                     /locus_tag="PLY83_04215"
     CDS             4833..5543
                     /locus_tag="PLY83_04215"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOV29278.1"
                     /translation="MVTDLQAIDPSLLTPIRNVVYDTLRQAILDGSIPPGERLVESEL
                     AETLNVSRMPVREAIRKLEAEGLVEHQPRKGAVVKAFSEEDILEIYTLREAMEALAAV
                     QAVRRATEEEIRRLEKAMDEIDRLCDLGEKADAAEVFEANRVFSEGVIAACHMPRLIQ
                     VINTYKEYLERFRRVTLADSSRRELVRREHRGILEAIRDRDEKRAEALTREHIRGALE
                     AYLKSIPEHRDRQAHPGR"
     gene            5640..6638
                     /locus_tag="PLY83_04220"
     CDS             5640..6638
                     /locus_tag="PLY83_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOV29279.1"
                     /translation="MKNTMRKALFPMIAALLLAGFFAGVASAAYPTKPFECIAPAGPG
                     GGWDTTMRMVTKVLTEKKLVTVPMPVINKPGGGGGVALAYLQKKKGRDDTLVVYSPPL
                     LLINLTGSTTLSYKNLTPISMLINDFGAFAVPKNSKYKTIKDVMEAIKKDPKSVKIGG
                     TSAMGSMDHIQFLVAAKAAGVKDLKAIQYIAFQGGEGIAALMGGHIDLLSTGMAETVG
                     PMQSGDIRVLAVSSPNRIKSGPMMTVPTLRESGIDAEFINWRGIFGPEAMPAPAVKYM
                     EDALAKMVKTPEWKEIAQRNGWTQVYMNAADFAKFLDKTNAEYKALLAEIGMLKAQ"
     gene            6708..7157
                     /locus_tag="PLY83_04225"
     CDS             6708..7157
                     /locus_tag="PLY83_04225"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HOV29280.1"
                     /translation="MRPNALVGFTAIFVGIVYAVQAFHLPKAMIGNPWAPVYFPIGLG
                     VLMAALGLLMVVLEARKGLTNLEGVKRPKFQASSLKLIFGAAALCILYALIFENLGFI
                     LSTLIFLGALLFLINGPRAWKLNTVLTVGFTFGAWYTFVKLFQINLP"
     gene            7174..8694
                     /locus_tag="PLY83_04230"
     CDS             7174..8694
                     /locus_tag="PLY83_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOV29281.1"
                     /translation="MEIFNNLMQGFAVAMTPLNLAWLLAGSILGTVLGMLPGLGPSTG
                     IALLLPMTFGMRPDTALIAMAAIYYGAMFGGSRSSILLNVPGDGAAVASCFDGYPMAL
                     NGQAEAALAISAIASFIGGLIATIAFVLVALPVARFALRFGPPEYFCLMVFALAATAA
                     ISKEALLKGVISLVLGLMLTTVGLDFQSGVPRFTFGSVELQMGIDFVVVIIGIYGISE
                     VFVNLEHIAQQITRPVQTKFGRIWISMAQWKRSIGPILRQTPVGFFVGVLPGAGGTIA
                     ALMAYNNEKQISKHPEEFGKGAIEGLAAPEAANNACSVGAMIPMMTLGVPGSGTTAVM
                     LGALLILGLQPGPLLFEQHPDVAWGVIASMFLGNIACAFINIPLAGLLVRVLSIPPKI
                     LYPLVVVLAFLGVYTINFSIMDFYLLVGLGFVGYLMKKFKVPTAPLILASVVGASMES
                     SFRQSLMLSDGSLSIFFRSGISNGLLIAAFLSLAWPFISDWRARKKREKSVDIPID"
     gene            complement(8771..9319)
                     /gene="cobO"
                     /locus_tag="PLY83_04235"
     CDS             complement(8771..9319)
                     /gene="cobO"
                     /locus_tag="PLY83_04235"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOV29282.1"
                     /translation="MEGTPGKKAVLERGLLEVYTGNGKGKTTAALGLALRMAGQGGRV
                     AFIQFMKGWDFYGEIEALKRIPGLRHERTGTPDYVYRGQESPVDRMEAQRGLDLAREC
                     LGGDYDLVVLDELNVVLDFGLLELQPVLDLLQSRPFHVEVVITGRGAPQALLDAADLV
                     TEMREVRHPYTKGVVSRRGIDY"
     gene            complement(9421..>9797)
                     /locus_tag="PLY83_04240"
     CDS             complement(9421..>9797)
                     /locus_tag="PLY83_04240"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOV29283.1"
                     /translation="GPKGGPGMREMLTPTSSLAGMGLDKSVALITDGRFSGASRGAAI
                     GHVSPEAAAGGPIALVREGDTIAIDIPGKRLDLLVDEAELERRKAEWTPWVQPVESGF
                     LNRYRAFVTSGVTGAVLKEGTF"
BASE COUNT         1691 a         3267 c         2809 g         1930 t
ORIGIN      
        1 ctatgcggcc caacgccctg gtgggatttg ccgccgcaat cctgggcggt ctttatacaa
       61 tccaggtgca tcgcctgccg aaagcaatcg caggcgatgc ctcaagccct gtttctttcc
      121 ccctcgcact gggccttctg atgaccgtcc tggggattct cctggtcctt caggaagccc
      181 ggcatggact gacaaacctg gaaggcgtca agcggcctgc tttcacggcc ccaagcctga
      241 ggaccatctt caggactgtg gcggcaagtc ttctttatgc ctttcttttc gagcgcctgg
      301 gatttttcgt ttcaacgctg ggctttttgg gcaccctgct ctatctgata aacggccgtc
      361 gggcctggaa gctgaacgcc gctctcgccc tgggcttcac gctcggagcc tgggcccttt
      421 tcgtccagct tttcgagctc ccgctgccct aggaaggaac gggtcatgga attcggatct
      481 ctgctcctga aaggcttttc cgttgccctg accccagtga acctggcctg gcttctggga
      541 ggatccatcc tcgggaccct tttcgggatg atcccggggt tcggcccctc taccggaatc
      601 gccctgctgc ttcccctatg cctcggcctg cggcccgaga cggccttgat cgccatggcg
      661 gccatctact acggcgccat gtttggcggc tcgagaagct cgatcctcat gaacgtgccc
      721 ggtgacagcg ccgccgtggc atcctgcttc gacggttatc ccatggcccg aaacgggcag
      781 gcggaagccg ccctggcggt ttccgccgtc gcctcgctga tgggcggtct ctttgcctcc
      841 gtcgccttca cccttcttgc tcttcccgcg gcccgcatcg ccctgcgctt cgggcccccg
      901 gaaaccttct gcctgatggt cttctccctg gcggcaacag cggcggtttc gagggaaaat
      961 ctcctgaagg ggctcatttc cctggttctg ggcctgatga tttccaccgt cgggctcgat
     1021 tccccgtcgg gggtttcgcg ctttaccttc ggcctcgagc agcttcagac gggattctct
     1081 ttcgtcgtcg tgatcctcgg catctacggc ttcagcgagg ttttgatcaa cctggagacc
     1141 ctcgcgctgg aaacggcccg gccgctacag ctcgaattca ggtggatcca gatctccatg
     1201 gcccattgga gacgttcgat cggcccgatc ctaaggcagt caccggtcgg ctttctcatc
     1261 gggatccttc ccggggccgg ggccgggatc gcggccttga gagcttaccg gaacgagaaa
     1321 aaacgctccc cccaccccga agagttcggg aagggcgccg tcgaagggct ggctgcaccc
     1381 gaggcggcca acaatgcctg ctcgatcgga gcgatgcttc ccatgatgac cctgggcgtt
     1441 cccggatcag gcaccgcagc cgtcatgctc ggcggcctgc tgctctgcgg cctgcagcct
     1501 ggcccccttc tgttcgagcc gcagccgggt atcggctggg ccgtcatcgc cagcctgttt
     1561 ttggggaact tcgcctgcgc ggtgatcaac cttccgctgg caggattctt ggttcggatc
     1621 ctgtcaatcc cgccgaaagc cttctatccg tttgtggtgg cggtttcttt tctgggcctc
     1681 tacacggtca atttcagcgt cacggacctt tacctgctgg tcgggatggg gatttgcgga
     1741 tatctgatga aaaagttccg gatccccacc gggcccctgg tcctggcttc cgtagtcgga
     1801 agctcggtgg aatcgtcgtt cagacagtct ctcgagcttt ccggcggctc cccttcgatc
     1861 ttcttccgat cttcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnctggac
     1981 tgaaaccgaa cctggttcgc tattccttca cccggtagat gctgtcgagc agggtcccgt
     2041 cacggtactc cacaacggct acgacccggt ccctgtccat ctcgacgggc tccgggacac
     2101 cggtgaaccg ctcgacttcc ttcttcaggt ccgttatctc cttcaggggc aggttggcct
     2161 tcttcgccgc ctcccagaga tccgcccggt tggggttgat gcagataccg cgttccgtga
     2221 cgatggcatc caccgtctct ccgggagtga cgagggtctg gacccggtct ttgatcgtcg
     2281 ggaccccgcc ccggaaggaa ggggccacca cgatggccat cttcgccccg gccgcggtgt
     2341 cgctatgccc ccctgaagcc ccgcgcagca cgccgtcgtt gccggtcatg acattcacgt
     2401 tgaagtccgt gtccacttcc aaggccgcca ggatcacgac atccaggttg tgaaccaggc
     2461 atccctggtt catcgggttg gcataccatg agggatcgat ctccatgtgg gcgcggttct
     2521 tgaccatcga gccggttact gccgaatcga agctctggac gtcgaaaagc gcccgaaaga
     2581 gcccttcttc cagcatctgg gccatgtagc ccgagatgcc gcccaacccg aatccgccct
     2641 ggatcccctt ctccttcagg tgatccctga cgaaagccgc gaccgcaagg ctcgcgcccc
     2701 cagctcccac ctggaacgag cagcccggag cgaggatccc cgaggcggca agaaggcgat
     2761 aggcattctc cgcgaccttc aagtcaagcg ggtttcgcgt gatgcgcgca gcaccggatg
     2821 cgatttttgc cggatcccca aggctgtcga caacgacgac ctggttcacc aggtgctggg
     2881 gtaccgagat gtggggatgg aggggatact cgaccaagtt gtccgtcacg gcgatgacgt
     2941 gcctcgcgaa acgcgcgtcg gtgatcgcgt accccagcga cccgcaggcg gatttcccgg
     3001 agcagcctgc gctgttgccc agcgcgtcgc aggccggtgc ggccagaaag gccacatcga
     3061 tcacgatgga cccctcctcg atggccctgg cccttccgcc gtggctccgg atgatgaccg
     3121 tctcttccat ttctcccctg gagatcatgc ggcccacttc gccgcgtgcc ccggaggtcc
     3181 agatccggcg aatgagcccc tttcggacgt aatccgcgac acagtcgtgc gagtcgggaa
     3241 tcgagctcgg tgcgaaggtg aggtctctga accccatttg gaggagcgtt tcgagcacca
     3301 gggggatgac cttgtcgccg tttctcaggt gatgatggaa cgagatggtc atgccgttct
     3361 cgaggcccga acgctcgatc gccgtcttca ggctaggcgc caccttgctc ctgcttggcg
     3421 gttcgggctt ccggaccggg taggcagcgg gccatggggc ccctttcagg acgcgggcga
     3481 aggcaccctc ataggggctg aaggccccca ctccgtcgac cccgacagga accagccgtc
     3541 ccagggcgtt cttaacgaag ttcattgagc ttccccccct tccatgccgt aaagcccggc
     3601 caggctgatg aggtggcggg cccgaacgat gatgggcgcg tcaaccatct tcccgtccac
     3661 ggccaccacg ccgcgtccct ggcgctccgc ctcggcagcc gccgcgacga tgcgctccgc
     3721 cttccggact tccttcgcgt cggggcggaa agcccggtgg atgacctcca cctggctcgg
     3781 gtgaatggcc gccttgccgg tgaagcccag ttccacgatc tgcctcgcct cccggaagag
     3841 cccttccggg tcgttgaaat cggtccatac cgtgtcgaag gccgcgatgc cggccgcctt
     3901 ggccgccatg accacccggc tgcgggcgta gaacaactcc caaccgtcgc gcgttttctg
     3961 gattcccatg tcggctgtca ggtcctgccc gccgagggtc agggccgtca cacgggagct
     4021 cgccgtagcg atcgcgtagg cgttttcgat gccccgtgcg gactcgagca tggcgtggat
     4081 ggggacgagg cccccggaca tgccgtgcct tttctcgatc tccgtcattc tcgaatcggc
     4141 cagcaggaca tcggagggat cctggcactt gggcaggcga atcgcaaaag gcttgagggg
     4201 aacgatcgcc tcgaggtctt cctcgaaaaa gggcgtatcc gccccgttga tcctgacgac
     4261 gaccgggaga tcgccgaaat cgtagtgcct caggaaaccg gccgtgatgt ccctggccgc
     4321 atccttctcg gacagcgcaa ccgcgtcctc caggtcgatc atgaccatgt ccgccccgaa
     4381 aaccgccgcg ttctggatca ttcccggggc gttacccggg atgtagagca tcgacctcat
     4441 gcccctcatg ggatcaaacc tccccaaccg cccgcctgag ggcggtttcg agccgtgccc
     4501 ggatcaccag ctccagggcc ccctggtcct ggatcgtgac ctgcattccc gaaaagcccg
     4561 ttctctcgag ggtttccatc accagcttcc gggtccgttc cccgaaaagc ttccggttca
     4621 ctccccggta atccagcctg aattcttccg ccgggaccac cgtcaccagg caatcggcgg
     4681 attccagggt tcccgcttgc gccatccgta caaccaattc gaccaccgcc cctgaatagg
     4741 cttcttaaaa ttgacaatcc ggaaaaaacc aggtaaattt taccatagga tcctggattt
     4801 aggatcccgt atccaaaaag gaggctccct gcatggtaac cgatctccaa gccatcgacc
     4861 cgagcctgct cactcccatt cgcaacgtcg tctacgacac tctccggcag gcaatattgg
     4921 acggttccat tcctcccggc gaacgcctgg tcgagagcga gcttgcggaa accctcaacg
     4981 tcagccggat gccggtccgg gaagccatcc ggaagctgga ggccgagggg ctcgtggagc
     5041 accagccccg gaagggtgcc gtcgtgaagg cgttcagcga ggaagatatc ctcgagatct
     5101 acacgctgcg ggaagccatg gaagcccttg cggccgtcca ggcagtccgc agggcaaccg
     5161 aggaagaaat ccgcaggctg gaaaaggcca tggacgagat cgaccggctt tgcgacctgg
     5221 gggaaaaggc ggacgccgcc gaagtcttcg aggccaaccg cgtgttttcg gaaggagtca
     5281 tcgcggcctg ccacatgccc cggctgatcc aggtcatcaa cacctacaag gaatacctcg
     5341 aacggttccg gcgcgtcacc ctggcggatt cttcccgaag ggaactcgtc cggcgtgagc
     5401 accgggggat cctcgaagcg atccgcgacc gggacgaaaa gcgggccgag gccctgaccc
     5461 gcgaacatat caggggagcc ctcgaagctt acctcaagag tattccggag caccgggacc
     5521 gccaggccca cccgggccga tgaacggggg gatggaagaa gacctggcca agagagcgaa
     5581 ccatcgcggc gcacccaagc ggcttttcca taccaagaca gatagagggg ggtatcctga
     5641 tgaagaacac gatgcgcaag gccctgttcc cgatgattgc agcactgctt ttggccggct
     5701 ttttcgccgg tgtagcctcg gcagcctatc ccacgaagcc gttcgaatgc atcgctcctg
     5761 ccggcccagg cggcggctgg gacacgacga tgcggatggt caccaaggtc ctgaccgaga
     5821 agaaactcgt gaccgtcccc atgccggtca tcaacaagcc cggcggcggc ggcggcgtcg
     5881 ccctggccta cctgcagaag aagaagggaa gggacgacac gctcgtcgtc tactccccgc
     5941 cgctcctcct gatcaacctg accggctcca ctacgctgag ctacaagaac ctcactccca
     6001 tttccatgct gatcaacgac ttcggggctt ttgccgttcc gaagaactcg aagtacaaga
     6061 ccatcaagga cgtcatggaa gcgatcaaga aagaccctaa gagtgtaaag atcggcggca
     6121 cctccgccat gggcagcatg gaccacatcc agttcctcgt ggcggccaag gcggcgggag
     6181 tgaaggacct gaaggccatc cagtacatcg ccttccaggg cggtgaaggc atcgcggccc
     6241 tcatgggcgg gcacatcgac ctgctttcca ccggcatggc cgaaaccgtc ggccccatgc
     6301 agtccggaga catcagggtc ctggccgttt cctcgcccaa ccgtatcaag tccggcccca
     6361 tgatgaccgt cccgacgctt agggaatccg gtatcgacgc cgagttcatc aactggcgcg
     6421 ggatcttcgg ccccgaggct atgccggccc ctgccgtgaa gtacatggaa gacgccctgg
     6481 ccaagatggt gaagaccccc gaatggaagg aaatcgcgca gcgcaacggc tggacccagg
     6541 tctacatgaa cgcggccgac tttgcgaagt tcctggacaa gaccaacgcc gaatacaagg
     6601 ccctgctggc cgagatcggc atgctgaagg cccagtaaaa aacttgacct gcaagggcga
     6661 gcccgtcgga ccgggctcgc cctcctttca attcaaggag tgatcccatg cgacccaatg
     6721 cactggtcgg attcaccgcc atcttcgttg gcatcgtcta cgcggtccag gctttccacc
     6781 ttccgaaggc catgatcggg aacccctggg cccccgtcta tttccccatc gggctgggcg
     6841 ttctcatggc agccctcggt cttctcatgg tggttctcga ggcccgcaag ggcctgacaa
     6901 acctggaagg cgtcaagcgc cccaaattcc aggcttccag cctcaagctg attttcggtg
     6961 cagccgctct ctgcatcctt tatgccctga ttttcgaaaa cctgggcttc atcctctcca
     7021 cgctgatctt cctgggtgcc ctcctgttcc tgatcaacgg ccccagggcc tggaaactca
     7081 acacggtcct gacagtgggc ttcaccttcg gagcctggta caccttcgtc aagcttttcc
     7141 agatcaacct gccgtagggg aggggagttg gacatggaaa ttttcaataa cctgatgcaa
     7201 ggcttcgccg tcgccatgac ccccctgaac ctggcctggc tgctcgccgg ctccatcctg
     7261 gggaccgtcc tggggatgct gccggggctt ggcccctcca cgggcattgc cctgcttctg
     7321 cccatgacct tcgggatgcg ccccgacacg gccctgatcg ccatggccgc catctactac
     7381 ggggccatgt tcggcggctc gaggagctcc atcctcctga acgtccccgg tgacggggca
     7441 gccgtcgcct cctgcttcga cggttacccc atggcgctaa acggccaggc ggaagccgcc
     7501 ctggccatct cggccatcgc ctcgttcatc ggcggcctta tcgccacgat cgccttcgtc
     7561 ctcgtcgccc tgccggtggc ccggttcgcc cttcgtttcg ggccgcccga gtatttctgc
     7621 ctgatggtct tcgccctggc ggcaacggcc gccatttcca aggaagccct gctcaagggc
     7681 gtcatttccc ttgtcctcgg cctgatgctc accacggtgg gactggactt ccagtccggc
     7741 gtgccgcgct tcaccttcgg ttctgtcgag ctccagatgg gaatcgactt cgtggtcgtc
     7801 atcatcggca tctacgggat cagcgaggtc ttcgtcaacc ttgagcatat cgcccagcag
     7861 atcacccggc cggtccagac caagttcggc aggatctgga tctccatggc ccagtggaaa
     7921 cgcagcatcg gcccgatcct gcggcagaca ccggtcgggt tcttcgtggg ggttcttccc
     7981 ggcgcagggg ggaccattgc ggcgctgatg gcctacaaca acgagaagca gatctcgaag
     8041 cacccggagg agttcggcaa aggcgccatc gagggcctgg ccgccccgga agcggcaaac
     8101 aacgcctgct cggtcggggc catgatcccg atgatgaccc tgggggtccc cggatccggg
     8161 acgaccgccg tcatgctggg cgccctcctg atcctgggcc ttcagcccgg ccccctcctt
     8221 ttcgagcagc acccggacgt cgcctggggc gtcatcgcca gcatgttcct ggggaatatc
     8281 gcctgcgcgt tcatcaacat tccgcttgca ggcctgctgg tgagggttct ttcgatcccc
     8341 ccgaagattc tctatcccct ggtggtggtt ctcgccttcc taggggtcta cacgatcaac
     8401 ttcagcatca tggatttcta cctcctggtc ggcctgggtt tcgtggggta cctgatgaag
     8461 aaattcaagg tcccgaccgc gcccctcatc ctggcctcgg tggtgggcgc atcgatggag
     8521 tcgtccttcc gccagtccct catgctttcg gacgggtcgc tttcgatttt cttccgctct
     8581 gggatctcca acgggctttt gatcgcggcc ttcctttccc tggcgtggcc cttcatctcc
     8641 gactggcgcg cccgtaagaa aagggaaaaa tcggtggaca tccccatcga ttagcgtgaa
     8701 tcggaagagg ggagagggaa ccggccaagc cggttccctc tcccctcttt atcccccgac
     8761 tttttcctcc tcaataatcg atcccccgcc tggaaacgac tcccttcgtg tagggatgcc
     8821 gaacctcccg catctcggtc acgaggtcgg cggcatcgag cagggcttgc ggagcccccc
     8881 tgcccgtgat caccacttcc acgtggaaag ggcgagactg gagaagatcc aggacgggtt
     8941 gcagctccag gagaccgaag tccaggacca cgttcagctc gtcaagcacc accagatcgt
     9001 aatccccgcc gaggcattcc cgggcgagat ccagtccacg ctgcgcctcc atccggtcaa
     9061 ccgggctctc ctgtccccgg tagacataat ccggagttcc cgttctctcg tggcgcagcc
     9121 ccgggatccg tttcagggcc tcgatctccc cgtagaaatc ccagcccttc atgaactgga
     9181 taaaggccac ccgtcccccc tggcccgcca tgcggagcgc caaccctagg gcagccgtcg
     9241 tctttccctt gccgtttccc gtgtaaacct ccagcaagcc gcgctccaga accgcctttt
     9301 tcccgggcgt cccttccatt tttcccgcct ccttccaggc cattgtattc ctggggatca
     9361 aaaaaagcgg gggggcggcg cctgaccgcc cccccattga aacggttccg tcggtttctc
     9421 ctagaaggtc ccttctttca atacggcacc ggtcactcca gaggtaacga aggccctgta
     9481 gcggttgagg aacccgctct ccaccggctg aacccaggga gtccactcgg ccttcctgcg
     9541 ctcgagctcc gcttcgtcca ccaaaaggtc caggcgtttc ccgggaatat cgatggcgat
     9601 ggtgtcgcct tcgcggacca gggcaatagg gcccccggcg gccgcctcgg gggaaacgtg
     9661 cccaatggcc gcaccccggc tggccccgga aaaccggccg tcggtaatca aggcgaccga
     9721 tttgtccagg cccatccctg cgagggagga cgtgggggtg agcatttccc gcatccccgg
     9781 gccccccttg ggcccct
//
LOCUS       DAOOVN010000032         9783 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_4391_length_9783_cov_14.319079, whole genome shotgun sequence.
ACCESSION   DAOOVN010000032 DAOOVN010000000
VERSION     DAOOVN010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 9783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9783
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4391_length_9783_cov_14.319079"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PLY83_04245"
     CDS             complement(<1..287)
                     /locus_tag="PLY83_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOV29284.1"
                     /translation="MKRTFVFFLFLAFSVSLPLAPAAASSGAIPETGAVVLALSGGGM
                     KGLAHIGVLDVLRKSGIPVAGIVGTSMGSIIGGLSACGYAPEEIEEIVRGL"
     gene            425..1648
                     /locus_tag="PLY83_04250"
     CDS             425..1648
                     /locus_tag="PLY83_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HOV29285.1"
                     /translation="MTESRKRMPLIWKIAIGFALGIVAGAVLKDKAQVLAPIGKIFIT
                     LLKMLIVPLVFSSLVVGTASLGDPRKLGRIGAKTIALYLVTTAVAIVIGLVLGNVLQP
                     GSGMALSVDTSGFKAPVPPSIIDVLLGIFPDNIAKSLTEANMLQIIVFALFFGVSAVL
                     AGEKGKPVIALMDSVAEAMYKLTGIVMAFAPYGVFALIATTVAKYGLSVLAPFAKVIL
                     AVYLGCFLHALLVYSGLIMLVVKKSPLWYFSGVRESTLTAFVTRSSSATLPVTMRVAQ
                     ENLGVSEGIASFVLPLGATINMDGTALYQGVCALFIAQAYGIPLSMGAQASIILTATL
                     ASIGTAGVPGAGLIMLTLVLTAVGLPVEGVALVAGIDAVLDMARTAINVTGDSCVAAV
                     VAKTEGELRDVERSL"
     gene            1632..2330
                     /locus_tag="PLY83_04255"
     CDS             1632..2330
                     /locus_tag="PLY83_04255"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HOV29286.1"
                     /translation="MNAPFKLIGVLGGMGPAAGAEFLRLLANFCPAGSDQEHPPVILL
                     SDPQIPDRTAALLGRGEDPTERIREDLFKLVFWGADLLAVPCNTAHAFIDRFAGELPV
                     PLVHIVEATLQEAIEKSPNGGWLIATEGTLASGLYQERARRKGYVLHLPSALEQDEVT
                     EIIRLVKANRISEAAGRFRPLAERLWRQAALPLITACTETPLAYRAAGLPESMTVSSL
                     EALARATLSKFAGK"
     gene            complement(2343..2816)
                     /locus_tag="PLY83_04260"
     CDS             complement(2343..2816)
                     /locus_tag="PLY83_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOV29287.1"
                     /translation="MEILLALDPGKDKFGWAFVTPGGDLLASGIIPRPHLQELLDGLL
                     AGDLGQLSGTIRERRGSGEKLPPPGKILLGSGTARSLLEEALTARGRNFTLVDEAYTT
                     LEARPLFWKLHPPGFFAGWIPQSLRVPPRPIDDLAAWALALRFLRDASEKPAGKG"
     gene            complement(2821..3699)
                     /locus_tag="PLY83_04265"
     CDS             complement(2821..3699)
                     /locus_tag="PLY83_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOV29288.1"
                     /translation="MRRQKAAWLGIRTFETIARFLPHSAAVRLGSWLGVLAWLFSWKR
                     VDAAERRCVRVLGVGVTEARRIVRESYRNHGRCAAEILRLPILEGALDNLFSVLGEEN
                     LRKAFEKGKGVILLSAHLGNWELAAAKIARMGFPVEAIGAEQRDPRITELLFSLRASG
                     GVGTIRKGLELKGALRCLQAGKILAVLLDQDAKHKGVVVPFLGRPASTPYGPVKMCWK
                     LGAPIVPVFPIRKNDGIHFDLVFLPALDLPPEEGGEGSLVNAVSLCNEVLGEWIARFP
                     EQWMWLYPRWASTVGD"
     gene            complement(3696..4976)
                     /locus_tag="PLY83_04270"
     CDS             complement(3696..4976)
                     /locus_tag="PLY83_04270"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOV29289.1"
                     /translation="MDLRSFFEQEVKPALGCTEPGAVALAAATAAKTLAQPVETIRLR
                     LSANLFKNGISVGIPGTRGLRGNLLAAALGALCGDPEKGLLVLEDVPEEALETARKMV
                     EEGLVSQEVVPDVPSVFVEAFLFGGGHSASAVIAGRHDRVVEVKADGEVIRRLLEADR
                     NLQGLPSYIQELMGKDMENLWDLAGSIDREIEDFLLEGVRMNRKVAQQGLESPWGLGV
                     GYSLAGSGEDLLYRIKALAGAAADVRMDGGSWPVMSSAGSGNHGITAILPVAAVAEAW
                     GKSPRELAEALALSHLVTGMIKAHTGRLTPTCGCVIAAGGGAAAGIVRLGGGTPRQAE
                     TAVASLVSSLLGVLCDGAKGSCALKVSAGAGEAYLSARMALEGRGVSDRQGVILPGLK
                     ETAQAVAEISRHGMAAVDATVLRLLGEMEGAARS"
     gene            5101..>5345
                     /locus_tag="PLY83_04275"
     CDS             5101..>5345
                     /locus_tag="PLY83_04275"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOV29290.1"
                     /translation="MIRIASLTLYIVMILAGWAFPLLGVVLLAGMGIVVLSGKRKKWC
                     GSYCPRGSFLDLVMSKVSPKKPIPAFLRSKKTWAAGLA"
     assembly_gap    5346..5445
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5446..6512)
                     /locus_tag="PLY83_04280"
     CDS             complement(<5446..6512)
                     /locus_tag="PLY83_04280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HOV29291.1"
                     /translation="MPPIMGAGAFIMSEFLGISYLRIATAAIIPALLYYTAIFVNVHV
                     RARKQNLKGLSRDELPVIREVLKKDGHLLIPIAVVIATLLMKYTPVVAAFFGVVSVVV
                     VSQLRPHTRMGLKNILEAMEEGARGSLGVAMACALVGFVVGTSSLTSLGLTISNNIIE
                     LSGGKLFPTLIMAMVASLVLGMGLPTTANYIVCSTIVAPALIGMNVLPLAAHMFVFYF
                     GIMADLTPPVCLAAFTGAGIAGAQPGKTGITATKIALVAYFIPYSFIYTPMILLQAPN
                     LVHLVILVGSSLLGIVSLAASLEGWLFRELKAVERAALLPLAVATFMPQVSVNLVSGG
                     LLAVLFFLLKKTAKPETALAE"
     assembly_gap    6561..6660
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6661..7253)
                     /locus_tag="PLY83_04285"
     CDS             complement(<6661..7253)
                     /locus_tag="PLY83_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter permease"
                     /protein_id="HOV29292.1"
                     /translation="MAPGETNTRASVLDTAPMDEAEVQKLVEKYDVESRYRRLSGMPA
                     KLLKAWLVAMTAFHLYTASIGVLPIAIIRAVHLTFAIVAVFILYPATQKGSKTSVPWY
                     DWILAGLSCGVIGYIVVMFNDISRRGALPLPYEIVLGAAAIVLILEGGRRIVGNVLPV
                     LSSLFLLYCYFGRVMPGMFMHRGYSLSRIIQHMYLTP"
     gene            complement(7344..8300)
                     /locus_tag="PLY83_04290"
     CDS             complement(7344..8300)
                     /locus_tag="PLY83_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HOV29293.1"
                     /translation="MKRVLQFLAVSCILMGLLGTSALAATTFLNIGTGSTGGTYYPVG
                     AAMCKIWNDKIPGLKASAQSTGGTVHNLQLLANKEAEAGFLDGLYYFAYQGKGKYEGN
                     PQSYLRALVPLYAEPVHILVAKGSGIKSLRDFKGKRVSVGAVASGTEVTARELLKIAG
                     LNPDKDIKPENLGLSDTAQAFSNKQIDAAITVGAVGIAGVVEAATMGVVEFIGLPEGQ
                     IKQANGRMPYYVPFTIPANTYKGQPEPIKTIASWNIIGVRQELDPDLAYKMTKALFEN
                     KKNLVNVSARMEGMSPENVKEILIPLHIGAKKYYKEIGAVTM"
     gene            complement(8428..9012)
                     /locus_tag="PLY83_04295"
     CDS             complement(8428..9012)
                     /locus_tag="PLY83_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HOV29294.1"
                     /translation="MRSRQKFPLRILSPLNSSAIADLTCGSPVLLSGSVLLARESALR
                     WMVEETQRGRTMPFDLNGMVLFHAGFTPASPGRILGSIGPTTSLRMDPWTPSLLKAGV
                     KGMIGKGKRSPEVVEAIREAGAVYFAATGGAAAFLSRSVRSFSFRMDSTFGAEAPALL
                     EVIDLPLIVAVDPKGNDLFEQGRRNFIKGTDQVQ"
     gene            complement(9015..>9783)
                     /locus_tag="PLY83_04300"
     CDS             complement(9015..>9783)
                     /locus_tag="PLY83_04300"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOV29295.1"
                     /translation="DVRAALQQALRNEKSEAGRRVLLELLENERIARCEKIPLCQDCG
                     LGVLFLEVGERVRFEGAGLLESVNEGVRLAVKEGFLRAMVVADPLYRRENTGDNTPAV
                     VHLERVPGETFRAVAFPKGMGAESGSTFTMLRPLDGEEAVVRAVLQTVERFGASACPP
                     LIVGVGIGGNFETAPLLGKRSLLRPLGRRNADPRYAHLEERLLEEVNGLGIGPAGYGG
                     TVTALDVHVEFAPTHSAGLPVAVNLCCHSLRRAEGTL"
BASE COUNT         1852 a         3071 c         2807 g         1853 t
ORIGIN      
        1 tccagtcccc gaacgatttc ctcgatctcc tcgggagcgt acccgcacgc agagagtccg
       61 ccgatgatcg atcccatgct cgtccccacg atcccggcta caggaatgcc ggacttacgg
      121 aggacgtcca aaacgccgat gtgggcaagc cctttcatcc ctccgccgga aagagccagg
      181 acgaccgcgc ccgtttccgg gatggcgccg ctcgatgcgg cggcaggcgc aaggggcagg
      241 gaaaccgaaa aagcgaggaa caggaaaaag acgaatgttc gtttcacgga cacccctcca
      301 ggacaggcag ccttggtgaa acaggaaaat cgttgctatc ttactccatg ccgggtgcag
      361 tttatcgtgt ataatggtag agaaacagtt aattattcat tttacaagga aaggggagta
      421 gaaaatgacg gaaagcagga agaggatgcc cttgatctgg aaaatcgcca tcggtttcgc
      481 cctcggcatc gtcgcgggag cggtcctcaa agacaaggcc caggtcctgg cccccatcgg
      541 caagatcttc atcaccctgc tgaagatgct catcgttccc ctggtctttt ccagcctggt
      601 cgtcggcacg gcttccctgg gcgatccccg gaaactgggg cgcatcggcg cgaagacgat
      661 cgccctttac ctcgtcacga ccgccgttgc catcgtgatc ggcctcgtgc tggggaacgt
      721 ccttcagccc gggagcggca tggccctttc ggtcgacacc tcgggtttta aagccccggt
      781 acctccttcg atcatcgacg tcctcctggg gatcttcccg gacaacatcg cgaaatcact
      841 caccgaggcg aacatgcttc agatcatcgt cttcgcgctg ttcttcgggg tttccgccgt
      901 tctcgcaggc gaaaagggga aacccgtcat cgccctcatg gactccgtgg ccgaagccat
      961 gtacaagctg accgggatcg tgatggcctt tgccccctac ggcgtcttcg ccctcatcgc
     1021 aacgacggtg gccaagtacg ggctatccgt ccttgccccg ttcgccaagg tcatcctggc
     1081 cgtctacctg ggatgcttcc tccacgcgct tttggtttac tcgggactca tcatgctcgt
     1141 ggtcaaaaaa tcgcccctct ggtacttctc gggtgtcaga gaatcgacgc tgaccgcgtt
     1201 cgtcacccgc tcgagctcgg caaccctgcc ggtgaccatg cgggttgccc aggaaaacct
     1261 cggggtttcc gagggaatag cttcctttgt cctgcccctg ggagcgacga tcaacatgga
     1321 cggcacggcc ctctaccagg gagtctgcgc gctcttcatc gcccaggcgt atggaatccc
     1381 cctctccatg ggagcccagg ccagcatcat cctgaccgca accctggcct cgatcggaac
     1441 cgccggggtg ccgggggcgg gcctgatcat gctgacgctc gtcctgacgg cggtgggact
     1501 gccggtggag ggagtcgccc tcgtggctgg catcgatgcg gtcctggata tggcaagaac
     1561 ggccatcaac gtgaccgggg acagctgtgt cgctgccgtg gtcgcaaaaa cggaagggga
     1621 gttgcgcgac gttgaacgct ccctttaagt tgatcggagt cctgggaggg atgggacccg
     1681 ccgcgggagc cgagttcctg cggctgctgg caaacttttg cccggccggg tcggatcagg
     1741 agcacccccc ggtgatcctt ctttccgacc cgcaaattcc ggaccggacc gcggctttgt
     1801 tgggccgagg agaggatccc acggaaagga tccgcgagga ccttttcaaa ctggtctttt
     1861 ggggagcgga tcttctcgcc gtcccctgca acacggccca cgcgttcatt gaccgcttcg
     1921 ccggggaact ccccgttcct ctcgtccaca tcgtcgaggc aacacttcag gaagccatcg
     1981 aaaaaagccc gaacggggga tggctcatcg cgaccgaggg aacccttgcc agcggcctgt
     2041 accaggagag agcgcgtcgc aaagggtatg ttcttcacct gccctcggct ctagagcagg
     2101 acgaggtgac cgaaatcatc cggctggtca aggccaaccg gatttcagag gccgcagggc
     2161 gcttcaggcc tcttgcggaa cggttgtggc gccaggccgc gcttccgctg atcacggcct
     2221 gcacggaaac ccctcttgca taccgggccg cgggcctgcc ggaatcgatg acggtttcca
     2281 gtctcgaagc gctggcaagg gcaacgctga gtaagtttgc cgggaaatag tcctgggtac
     2341 gctcatcctt tcccggcagg cttttccgat gcatctcgaa ggaaccggag agccagggcc
     2401 cacgcggcga gatcgtcgat gggccttggc ggcacccgca ggctttgggg gatccaccct
     2461 gcgaaaaaac ccggcgggtg aagcttccag aagagcggcc tcgcctccag cgtggtgtaa
     2521 gcctcgtcaa cgagggtgaa gttccgcccc cgggcggtga gggcctcctc caggaggctt
     2581 cgggccgttc cgctcccgag taggatcttt ccaggaggcg gcagcttttc cccgcttccc
     2641 cgtcgttcac ggatcgtccc ggacaattgc cccaaatccc cggcgaggag cccatccagc
     2701 aattcctgca ggtgcgggcg cgggatgatc ccgctggcca gcaggtcccc gccgggcgtg
     2761 acgaaggccc agccgaactt gtctttccca ggatcgaggg caagcaggat ctccatcgtc
     2821 tcaatccccc accgtgctgg cccagcgcgg gtaaagccac atccattgct ccgggaatcg
     2881 ggcgatccac tcccccagca cttcgttgca aaggctcacg gcgttcacga gcgatccctc
     2941 gccgccttcc tccgggggca ggtcgagggc gggaagaaag accaggtcaa aatggattcc
     3001 atcgttcttc cggatgggaa aaaccgggac gatgggggct cccagtttcc agcacatttt
     3061 gaccggaccg tacggagtac tggcgggacg tcccaggaaa gggacaacga cgcccttgtg
     3121 cttcgcgtcc tggtccagaa gaacggcaag gattttcccg gcctgcaggc agcggagggc
     3181 ccctttcagt tcgagcccct tgcggatcgt tcccactccc ccggaagccc gaagcgagaa
     3241 cagcagctcg gtgatccgcg ggtcgcgctg ttcggcaccg atggcttcca cggggaaacc
     3301 catcctggcg attttcgctg ccgccagttc ccagtttccc aggtgcgccg aaagcaagat
     3361 gactcctttc cccttttcga aagccttgcg gagattctcc tccccgagga cggaaaaaag
     3421 gttatccagg gcaccttcaa gaatcggcag gcggaggatc tcggccgcgc agcggccgtg
     3481 gttcctgtag gattcgcgga caatgcggcg ggcctcggtg actccaaccc cgaggacccg
     3541 tacgcaccgt ctctcggccg catccacacg cttccagctg aaaagccagg ctaaaacacc
     3601 gagccacgat cccagccgga ccgcggcgga atggggcaag aacctggcaa tggtttcaaa
     3661 cgtccggatc cccagccagg ccgctttctg cctcctcaag aacgggcggc cccttccatt
     3721 tcccccagaa gccgaaggac cgtggcgtcc acggcagcca tgccgtgacg gctgatctcg
     3781 gcaaccgcct gggccgtttc tttcaacccg ggaaggatga ccccctgccg gtccgataca
     3841 ccgcgccctt caagggccat ccgggcggaa aggtacgctt ccccggcccc cgctgaaacc
     3901 ttcagggcac aggaaccttt tgcgccgtcg cacaacaccc ccagaagcga ggaaacgagc
     3961 gaggcgaccg ccgtttccgc ctgtcgcgga gtcccccctc ccagccgtac aatcccggcc
     4021 gcggcaccgc ctcccgcggc gatcacgcat ccgcaggtcg gtgtgagcct gcccgtgtgg
     4081 gccttgatca tccccgtcac caggtggctg agagccaggg cttcggccag ttcccgaggg
     4141 cttttccccc aggcctcggc caccgccgcc acggggagga tcgccgtgat cccgtggttg
     4201 ccgctcccgg cgctgctcat gaccggccag gagccgccgt ccatccggac atccgccgcc
     4261 gcgccggcaa gcgccttgat cctgtagagg agatcttccc cgcttcccgc aagggaatag
     4321 cccacgccca gtccccaagg ggattccagt ccctgctggg ccacctttcg gttcatccgg
     4381 accccctcca gaaggaaatc ctcgatctcc ctgtcaatcg agccggcaag gtcccaaagg
     4441 ttttccatat ccttgcccat gagttcctgg atgtaagacg gaagcccctg aaggtttcga
     4501 tccgcttcga ggaggcgccg gatcacttcc ccgtcggctt tgacctcgac gacccggtca
     4561 tggcgtcccg caatcactgc ggaggcactg tgcccccctc cgaaaaggaa ggcttccacg
     4621 aaaacactcg gcacatcggg aaccacttcc tgggaaacga ggccttcttc caccatcttg
     4681 cgggctgttt ccaacgcttc ttccggcaca tcttcgagga caagaaggcc cttttcggga
     4741 tccccgcaaa gggccccgag ggctgcggcc aggaggtttc ccctcaagcc cctggtcccc
     4801 ggaatgccga cggagattcc gttcttgaag aggttggcag aaagccgcaa ccggatcgtt
     4861 tccaccggtt gagccagggt tttggcggca gtcgcggcgg ccagggcgac agcccccggt
     4921 tcggtgcagc cgagcgccgg cttgacttcc tgctcgaaaa aggagcgcaa gtccattccc
     4981 gtatcccctt tccacgtttg gatcctttcc tagggtatta tgcctcaaga ttccttgaca
     5041 agataccttt gatgcgatta atataccccc atatagtatt agtgccggag gtgttgaacc
     5101 atgatccgaa tcgcgagttt gaccctgtat atcgtgatga tcctggccgg ctgggcattc
     5161 cccctcctgg gggtcgtcct tctcgctggc atggggattg ttgtgctttc cggaaagaga
     5221 aagaaatggt gcggttctta ttgcccccgc ggcagcttcc tggatctcgt gatgtcgaaa
     5281 gtctccccca aaaagccgat tcctgcgttt ttgaggtcga aaaaaacctg ggcagccggg
     5341 cttgcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttttc ggccagcgcc
     5461 gtctcgggct tggccgtttt tttcagcagg aaaaacagca cggccagcaa tcctccagaa
     5521 acgaggttga cgctcacctg gggcatgaat gtcgcaacgg caagggggag aagagcggcc
     5581 cgttctacgg ccttcagttc cctgaacaac cagccctcga ggctcgccgc caggctgacg
     5641 atgcccaaaa gggaggagcc gaccaggatc acaaggtgga cgaggttcgg cgcctgaaga
     5701 aggatcatgg gggtatagat gaaagaatag gggatgaaat acgccacgag tgcaattttc
     5761 gtggcagtga ttcctgtttt ccccggctgt gccccggcaa tccccgcccc cgtgaaagcg
     5821 gccaggcaca cgggcggagt caggtcggcc atgatcccga aataaaagac gaacatgtgc
     5881 gcagcgagcg gcaggacgtt catgccgatc aaagccggag ccacgatggt gctgcagacg
     5941 atgtagtttg cggtcgtcgg caggcccatc cccaggacga gcgaggcgac catcgccatg
     6001 atcaatgtcg ggaagagctt tccgcccgaa agttcgatga tgttgttgga gatcgtcaat
     6061 cccagggaag tcagagacga cgtgcccacg acgaatccaa ccagggcgca ggccatggcg
     6121 acgcccaggg aacccctggc tccctcttcc atcgcttcca ggatgttctt cagtcccatc
     6181 cgggtgtggg gtcgaagctg gctgaccacg acgaccgaga ccacgccgaa aaaggcagcg
     6241 accacggggg tgtacttcat gagcagggtg gcgatgacga cagcgatcgg gatgagaaga
     6301 tgcccgtcct tcttcaaaac ctcgcggatg accggcagct cgtccctgga aagccccttc
     6361 aggttctgct tccttgcccg gacgtgaacg ttcacgaaaa tggccgtgta gtacaagaga
     6421 gccgggataa ttgccgctgt tgcgatcctg aggtaagaga tccccaaaaa ctcgctcatg
     6481 atgaaggccc cagcccccat gatgggcggc atcagctgcc ctcccgtgga ggcgcaggcc
     6541 tccacggccc cggcgtactc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 tccggcgtca ggtacatgtg ctggatgatt cggctgaggc tgtacccccg gtgcatgaac
     6721 atccccggca tgacacggcc gaaatagcag tacaaaagga aaaggctgct cagcaccggc
     6781 aggacgtttc cgacgattcg ccgccctcct tcaaggatca ggacgatggc ggctgccccc
     6841 aaaacgatct cgtagggcaa gggcaaggca ccccgtcgag aaatgtcgtt gaacatgacg
     6901 acgatgtagc cgatgactcc gcaggaaagc ccggcgagga tccagtcata ccagggaacc
     6961 gatgttttgg accccttctg ggtcgcaggg tagaggatga aaaccgcgac gatcgcgaac
     7021 gtcaggtgaa cggcccggat gatggcaatc gggagaaccc cgatcgaggc cgtgtacagg
     7081 tggaaagccg tcatggccac tagccaggcc ttgaggagct ttgccggcat tcccgacaat
     7141 cggcggtacc ggctttctac atcgtatttt tcgaccagct tctgcacttc ggcctcgtcc
     7201 atgggtgccg tgtcaagaac ggaggcccgt gtgttcgttt cacctggtgc caattcactt
     7261 tcactcctct cacaatctca agggggcggt gcggcttacc gcccgccccc ttcgacttca
     7321 aacgcccttc acccggtttg cggctacatc gtgacggcgc cgatctcctt gtagtatttc
     7381 ttggccccga tgtgaagcgg gatcaggatt tccttgacgt tctccgggct catcccttcc
     7441 atcctggcag aaacattcac gaggttcttc ttgttctcga agagggcctt cgtcatttta
     7501 taggccaggt ccgggtccag ttcctgccga acgccgatga tgttccagct ggcgatcgtc
     7561 ttgatcggtt ccggctggcc cttgtaggta ttcgccggga tcgtgaacgg gacgtagtaa
     7621 ggcattcttc cgttggcctg cttgatctgc ccttccggaa ggccgatgaa ctcgacgacc
     7681 cccatggtcg cggcttcaac gacgccggcg atgccgaccg ccccgaccgt gatggccgcg
     7741 tcgatctgct tgttggagaa cgcctgggcg gtgtcgctca gtccgaggtt ttcaggcttg
     7801 atgtccttgt cggggttcag tcccgcgatc ttgagaagct cgcgggcggt cacctcggtt
     7861 ccgctggcca cggccccgac ggaaaccctc tttcccttga agtcccttaa ggatttgatg
     7921 ccgcttccct tggcgaccag gatgtggacc ggttccgcgt agagaggcac cagggcacgc
     7981 aggtagctct ggggattgcc ttcatacttg ccttttccct ggtaggcgaa gtaatagagg
     8041 ccgtccagga agcccgcctc ggcttcctta ttggcgagaa gctgaaggtt atgcaccgtg
     8101 ccgccggtgg actgggcgct tgccttgagt cccgggatct tgtcgttcca gatcttgcac
     8161 atggctgccc cgaccgggta ataggtaccg cctgtgcttc ccgtcccgat gttcagaaac
     8221 gttgtcgccg ccagtgccga agtgcccagc aggcccatga ggatacagga aaccgccaga
     8281 aactgcaaaa ctctcttcac tttctctcca ccccctgcaa aatgggaaat ttttcttcga
     8341 tattgaagca tacaatccgg ggacgtgcaa ggtgttccgg tcatccggtc atcgtccgaa
     8401 gaggctcgct ttcatttacc aaaaatttta ttgcacctgg tccgtacctt taataaagtt
     8461 tctccgtcct tgttcgaaca agtcgttccc cttcgggtcg accgcaacga tcaggggcag
     8521 atcgatgact tccagcaaag caggggcttc cgccccaaaa gttgaatcca tccggaaaga
     8581 gaaggaacgg accgaccgtg acaggaaggc cgccgctccg cccgtggcag cgaaatagac
     8641 cgccccggct tcccggatgg cctcgaccac ctccggcgag cgtttcccct tgccgatcat
     8701 ccccttcacc ccggccttca ggagggaggg cgtccacgga tccatcctca ggctcgtggt
     8761 gggcccgatt gaaccgagga ttcttcccgg ggaagccggt gtaaaccccg catggaacag
     8821 gaccattcca ttcaggtcga agggcatggt cctccccctt tgcgtttcct cgaccatcca
     8881 gcgaagagca gattcccggg cgaggagcac cgatccggat aggagaaccg ggctgccgca
     8941 ggtcaaatcg gcaatcgcgg atgaattcag cggggacagg atcctcaagg ggaacttctg
     9001 acgggatctc atcgctagag ggtcccctcg gcgcgtcgga gcgagtggca gcaaaggttc
     9061 accgcaacgg gcagtcccgc agagtgggtc ggagcgaatt cgacgtgaac atccagggcc
     9121 gtcacggtcc cgccgtatcc ggccggaccg attcccagcc cgttgacctc ttcgagcagt
     9181 cgttcctcca ggtgcgcgta acggggatcc gcgttcctcc tccccagcgg gcgcaacagc
     9241 gacctcttcc ccagcagggg agccgtttcg aaattcccgc cgatccccac cccgacgatg
     9301 aggggcgggc aagcgctcgc cccgaaccgt tcgacggttt gcaggacggc gcggacgacg
     9361 gcctcttcgc cgtccagcgg ccgcagcatc gtgaaggtgc tcccgctctc cgctcccatc
     9421 cctttcggaa aggcaacggc ccggaacgtc tccccgggga ctctctcaag atgaacgacc
     9481 gcaggcgtgt tgtcaccggt gttttccctt cgatacagcg ggtccgccac gaccatggcc
     9541 cgaaggaagc cttcttttac ggccagtcgg acgccttcgt tcacggactc gagcaaaccc
     9601 gccccctcaa agcggaccct ttcccccacc tccaggaaga gcactccaag gccgcaatcc
     9661 tggcaaagcg ggattttttc acaccgggcg atccgttcgt tttccaggag ttccaagagg
     9721 accctccggc ccgcctcgct tttctcgttt ctcagggcct gctgcagcgc tgcccgcacg
     9781 tcg
//
LOCUS       DAOOVN010000033         9692 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_4444_length_9692_cov_14.910968, whole genome shotgun sequence.
ACCESSION   DAOOVN010000033 DAOOVN010000000
VERSION     DAOOVN010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 9692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9692
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4444_length_9692_cov_14.910968"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1831
                     /locus_tag="PLY83_04305"
     CDS             <1..1831
                     /locus_tag="PLY83_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOV29296.1"
                     /translation="WDELDFLVVSGDAYVDHPSFGHAVIARVLEREGYRVGILAQPDY
                     RSPGAFLALGRPRLGVLVSAGNLDSMLNAYTAAKKRRHTDSYSPGNKAGRRPERATIV
                     YCNRVRQVMGDVPVVIGGIEASLRRFAHYDYWDDAVRRSILVDSGADLLVFGMGELQI
                     VEIARQLAIGISPRDIRDVAGTCWMASEKDEVWKESVELPSFEAVQSSKTAFSEAFRL
                     FYLEQDPIRGKTLMQAHGSRWVVQNPPARPLTTEELDSVYELPYARAAHPDYDAEGGV
                     PAIQEVQFSLVSHRGCFGSCSFCAITSHQGRIIQSRSKESLLREAALLTGLPNFKGYI
                     HDVGGPTANFRHPSCEGQLERGTCRGRECLGPKPCPRLDADHSEYLDILRAIRSLPRV
                     KKVFVRSGIRYDYLLLGGLEPFLTELCEHHVSGQLKIAPEHSSKRVTDLMGKSGKEAY
                     LAFVEAYRRVNRRLGKEQYLVPYFMSSHPGSGLKEAVELAEFLRDTGIHPEQVQDFIP
                     TPGTLSTCMYATGIHPLTGERVYVAKSPREKRLQRALLQYRDPKNADLVREALERAGR
                     TDLVGLGERCLVRPRGFSRGVSKGKNRGRQEKEGKGHADRRAK"
     gene            1884..2303
                     /locus_tag="PLY83_04310"
                     /pseudo
     CDS             1884..2303
                     /locus_tag="PLY83_04310"
                     /inference="COORDINATES: protein motif:HMM:NF013766.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
     assembly_gap    2304..2403
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2404..2936)
                     /locus_tag="PLY83_04315"
     CDS             complement(<2404..2936)
                     /locus_tag="PLY83_04315"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOV29297.1"
                     /translation="MNSAKKWILALLAVFLLGYPAGVFASDTKQPGEWSPKPSERWLE
                     VVKKERRLYLRQGDQILKAYRVAIGSGRGIKKSPIDKITPVGVFTIRRIADARNWVFD
                     PKVFNEPGEPQKDVYGRWAISFKNPWNLAIHGTNAPWSIGKAVTHGCIRLKNEDIEEL
                     IKHVKPGMKLVIREKPLP"
     gene            complement(3070..3786)
                     /locus_tag="PLY83_04320"
     CDS             complement(3070..3786)
                     /locus_tag="PLY83_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV29298.1"
                     /translation="MSEAILKVCDIHCAYDAVPVIHGISLEVREGEIVAIVGANGAGK
                     STTMRTIAGLMHPVKGSIFFCGEDISRTPGHAMITKGISFVPEGRRLFSKLTVKENLE
                     LGAYTEKNRAVIEERLEEVLTLFPILRKRANQVAETMSGGEQQMCAIARGLMSNPKLL
                     MLDELSLGLMPSLVEKVLETVVEINRKGTTILLVEQMVQEALEIASRGYVIQTGRIVQ
                     EGTAKDLLESDSVRKAYMGL"
     gene            complement(3783..4538)
                     /locus_tag="PLY83_04325"
     CDS             complement(3783..4538)
                     /locus_tag="PLY83_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV29299.1"
                     /translation="MALLEVRNLTMKFGSLVANSDVSFDVEQGTIVGLIGPNGAGKTT
                     LFNCVAGYYKPTDGTIRFDGKDITNLPAYRISRLGMARTFQVVRPLKEMTVLENILVG
                     AYLREPDPEKAEAIAEKCLKTCFLGEFRDRPAGGLTIGNKKRLEVARALATGPKLLLL
                     DEAVAGLTSTEVKQMVELIKSLKESGVTILMVEHIMEAIMPIADRIVVLDGGVKIADD
                     RPQVVVEDENVIKAYFGEKFSKRLKAMKDGGRV"
     gene            complement(4540..5544)
                     /locus_tag="PLY83_04330"
     CDS             complement(4540..5544)
                     /locus_tag="PLY83_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOV29300.1"
                     /translation="MKTRKLETKDWIWIGLIGFLAALPLVPGLIGNSFRAHVLILVLL
                     YASMAQSWNIVSGYAGQVSFGHSVFFGIGAYGAGMAVVTYGLTPWPGLLAGAVLAALV
                     SLLISYPCFKLKGHYFAIATFAIVEIFNRLFMVWDWVGGALGLDYPILPDGWKNFVWS
                     ETKVGFYYGALGLFILIFGVVRWIETHRLGYYLRAVREGQETAESLGVNSAAVKLSAM
                     AFSAFLAALCGGFFVQYNYRVDPPMVMSLDMSMKFVLITILGGVGNFWGPLLGAAILV
                     PLQEYSRAYLTSLGSGVDLIIFGIIIMLMMIRQPKGIMGILQDLGFKTGQRKPGREEN
                     "
     gene            complement(5550..6410)
                     /locus_tag="PLY83_04335"
     CDS             complement(5550..6410)
                     /locus_tag="PLY83_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOV29301.1"
                     /translation="MSTLAQVLLDGLLAGMLYALVAAGLSLIWGVMDVINFAHGEFLM
                     VAMYLSYWIGFLAHVDPLFSWVASGVFVFLLGVLTYRFLVSRCIGKAAMAPLLATFGL
                     SMFLKNLCLNRFSPNFRILQSTLLEGKKILLGNIVIGMPQLVTGIFSLLLLFALYFLM
                     KKTRLGWAIQATAMDREAAELMGINTDRIYLLVFGLGGACVGIAGGLLTSYLAVHPEV
                     GSLFGLIAFLIVALGGFGSIPGALAAAVIIGLVEALAGFYIAPVFKYVAVFAIYLAVI
                     LVRPKGLFGW"
     gene            complement(6508..7767)
                     /locus_tag="PLY83_04340"
     CDS             complement(6508..7767)
                     /locus_tag="PLY83_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOV29302.1"
                     /translation="MQKRKFLSIVSLLVFALLLSAGSALAAENVLKIGVLFPLTGPCA
                     TAGQRCVAAVETAAEVINNKHPELDVPLAKQEGILKGMKIQLVVADTQGKPDVAKSEA
                     ERLVNQEGVYAIIGSYNSSASKPASAVTERAKKLFMCGCSSSAALTERDFKYFFRLAP
                     TDKTESKEFVEYIQWLNKNQKAGIKTIGLIYENTEFGKHAADEGKAAAKAAGFEVVAD
                     VAFNPGATNLNSEVQTLKAKNPDAVFGACLGGDYTLWVRTMKQMNWLPKVALNYCTGY
                     QDPKIAQQLAADGNFFMGGTGYSPEIGAKYMKKVAAVEKIYSKKVGVPFDSDSIQEAV
                     ALFILAQAIEKAKTLDVDKVAEVIRTTTFDSPLSLGGKVAFGPGGQNMKATSIITQLH
                     DGKYVPVYPPSYAETKATVPMTPWDKR"
     gene            8065..8466
                     /locus_tag="PLY83_04345"
     CDS             8065..8466
                     /locus_tag="PLY83_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOV29303.1"
                     /translation="MPEIIQVSEAVSLALHGLGLLVSRKGRISIREMAAEMGVSEAHL
                     AKVFQRLSKGGLVRSMRGPGGGFVLAKAPSDVTLYEVYSLIEGEPEVRICLLDCGKCP
                     FRKCLFEGLLVKMKQEFVNFLRKKRLSDLEE"
     gene            complement(8527..8826)
                     /locus_tag="PLY83_04350"
     CDS             complement(8527..8826)
                     /locus_tag="PLY83_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HOV29304.1"
                     /translation="MSEKWKDRLTDQLCKALLSLKSEDEAYRFLEDVATIGEIIAFAQ
                     RLEVARLLYEGNNYPQVVQKTGASTATISRAKNLIEHGADGFTLVLERLAKKEKE"
     gene            complement(9162..>9692)
                     /locus_tag="PLY83_04355"
     CDS             complement(9162..>9692)
                     /locus_tag="PLY83_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29305.1"
                     /translation="RKTRPGCGVMMSAGVVRLTLFQSRSAKTRLGPLVAALLILFAAN
                     GAVRLWKTISLRETRQTYDRLSAEARWLEVEVSGLEKRLREIQAQLEDGQAWITRLER
                     EILANQGKKRLEAQVRRAQAVERYRLLAGDYRRLYGEYQKAFEQLRGLHRSIEELADR
                     LDFQEEPPVRRKGATE"
BASE COUNT         1962 a         2764 c         2887 g         1979 t
ORIGIN      
        1 atgggacgaa ctcgatttcc tggtggtcag cggtgacgct tacgtggacc atccgagttt
       61 cggtcacgcg gtcattgcca gggtgctgga gcgggaagga taccgcgtcg ggattctggc
      121 ccagccggat taccgttccc caggggcctt tctggccctg ggacgcccgc gcctgggcgt
      181 gctcgtttcg gcgggaaacc tggactcgat gctgaacgcc tatacggctg cgaagaaacg
      241 gcggcacacg gatagctatt cccccgggaa caaggcgggg cgaaggcccg aaagggcaac
      301 catcgtctat tgcaaccggg tccgtcaggt tatgggcgac gttcctgtcg tcatcggggg
      361 catcgaggcc agcctgaggc gtttcgctca ctacgattac tgggacgatg cggtccggcg
      421 atcgatcctc gtggatagcg gggcagacct cctggttttc ggaatggggg aactgcagat
      481 cgtggagatc gcccgccaac tggccatcgg gattagcccg agggatatcc gcgacgtggc
      541 tggaacctgc tggatggcat cggaaaagga cgaagtctgg aaggaatcgg tggaacttcc
      601 gtcgtttgaa gccgtgcaaa gctcgaaaac ggccttttcc gaggctttcc gccttttcta
      661 cctggaacag gacccgattc gcgggaaaac cttgatgcag gcccacggat cgaggtgggt
      721 cgttcagaac cctcccgcaa ggccgctgac gacggaagaa ctggactcgg tctacgagct
      781 tccctacgca agggctgccc acccggatta cgatgccgag ggcggggtcc cggccattca
      841 agaggtccaa ttcagcctgg tgagccacag ggggtgtttc ggaagctgca gcttctgcgc
      901 catcacatct caccagggga ggatcatcca gtccaggagc aaggagtcgc ttttgaggga
      961 ggcggctttg ctgacagggt tgccgaattt caaggggtac atccacgatg tcggggggcc
     1021 gaccgcgaat ttccggcatc cttcctgcga gggacagctg gagcggggaa cctgccgggg
     1081 acgggaatgc ctgggcccaa aaccttgtcc gagactggat gccgaccatt ccgagtacct
     1141 ggacatcctg agagcgatcc gttcgcttcc ccgggtcaaa aaagtttttg tccgttctgg
     1201 gatccgctac gactacctcc tcctgggggg actggagcct ttccttacgg agctttgcga
     1261 gcaccacgtg agcggacagc tcaagattgc cccggaacac agttcgaaac gagtgaccga
     1321 cctgatggga aagagcggga aggaggcgta ccttgccttt gtggaggcgt accgacgggt
     1381 gaaccggagg ctgggaaagg agcagtacct cgttccttac ttcatgtcga gtcaccccgg
     1441 gtctggactc aaggaggcgg tcgaactggc ggagttcctc cgggacacgg gaatccaccc
     1501 ggaacaggta caggatttca tcccgactcc cgggaccctt tcgacctgca tgtacgcgac
     1561 agggatccac ccgctcaccg gggagcgggt ctacgtggcg aaaagtcctc gcgagaaaag
     1621 gctccagcgg gctcttttgc aataccggga tccgaagaac gcggatctgg ttcgggaggc
     1681 cctggaaagg gccgggagga cagacctggt cggtcttggg gagaggtgcc tcgtccgtcc
     1741 ccggggcttc tccagggggg tgtccaaagg gaaaaacaga ggacgccagg aaaaggaggg
     1801 gaaggggcat gccgatcgaa gggcaaaatg agaagcgcct tcaaacggct acatttgcgt
     1861 tgggctgatt ctggggtccc gatgcccggt tcgggagtct tgaaggagtg atccggacac
     1921 gcgtgggata tgccggggga acatcgccaa acccgaccta tcacgatctg ggggaccaca
     1981 cggaatccgt gcagatcgat tttgacccgg aagtcctatc tttttgccgg ctgctggaaa
     2041 tcttctgggc atcccacgat gccacccggc aggcctggtc acgccagtac gcttcgttga
     2101 tcttcgttca ttcgaaaacc cagcgggaag aggcggagga aagcctgaac cggcattcgg
     2161 gcaaaggaaa ccgtgccgtt accacggaga tccttccctt cacgggattc acccgggccg
     2221 agaatttcca ccagaaattc tacctgcggc gagatttcac cctgatggaa attttcagga
     2281 agcttttccc tgaagagcgg gccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnggaagag gcttttccct gatgacaagc ttcatcccgg gcttcacgtg cttgatcagt
     2461 tcctcgatgt cttcattttt cagccggatg catccatggg tgaccgcctt tccgatcgac
     2521 cacggggcgt tcgtcccatg aatcgccaga ttccagggat tcttgaagct gatggcccac
     2581 ctcccgtaga cgtccttctg cggttctccg ggttcgttga agaccttcgg gtcgaacacc
     2641 cagttgcggg cgtccgcgat gcgcctgatc gtgaaaaccc cgaccggtgt gatcttgtca
     2701 atcgggcttt tcttgatgcc cctcccgctg ccgatggcga cacgataggc cttcaatatc
     2761 tggtcaccct gccgaaggta gaggcgccgt tctttcttca ccacttcaag ccatcgttcg
     2821 gagggcttgg gagaccattc ccccggctgt ttcgtgtcgg aggcgaaaac ccctgccggg
     2881 tagccgagaa ggaagaccgc cagaagggcc aggatccatt ttttcgcaga attcaagaca
     2941 caagtccccc cactctgatc ctcaccggtt ttcttttagg ataccagcag atcgatcgct
     3001 ttgcatccat ggaaaaggca ggcctcgaaa acggaggcct gccttccaga cagcaccggc
     3061 gaaccaaacc tacagtccca tgtaggcctt gcggaccgaa tccgactcca gcagatcttt
     3121 cgccgtccct tcctgaacga tccgccctgt ttggatgacg tacccccggg aagcaatttc
     3181 aagggcttcc tggaccatct gttccacaag caggatcgtc gttccctttc ggttgatttc
     3241 cacgacggtt tcaaggacct tttcgacgag agagggcatc agtccaaggg agagttcgtc
     3301 cagcatgaga agtttcgggt tcgacatgag tccccgggcg attgcgcaca tctgctgctc
     3361 tccgccgctc atggtttctg ccacctggtt ggcccgtttc cgaaggatcg ggaaaagggt
     3421 cagcacttct tccaggcgtt cttcgatcac ggctcgattc ttctccgtgt aggcacccag
     3481 ctccaggttt tccttcactg tcagcttgga gaaaagacgg cggccctccg gcacgaaact
     3541 gatccccttg gtgatcatgg catgcccggg agtcctggaa atgtcctccc cgcagaagaa
     3601 gatcgagccc tttaccgggt gcatcagccc ggcgatggtc ctcattgtgg tcgatttccc
     3661 cgccccgttg gccccaacaa tcgcgacgat ctcgccttcg cggacctcga gggaaatgcc
     3721 gtggatgacg gggaccgcgt cgtaggcaca atggatgtcg cagactttca ggatcgcttc
     3781 gctcataccc gcccaccgtc cttcatggct ttcagccgct tgctgaactt ttccccgaag
     3841 taggccttga tgacgttctc gtcctcgacg acgacctggg gccggtcatc cgcgatcttg
     3901 accccgccgt ccagaacgac aatccggtcc gcgatcggca tgatcgcttc catgatgtgt
     3961 tcaaccatca ggatcgtaac gccgctttcc ttcaggcttt tgatcagttc gaccatctgc
     4021 ttgacctcgg tcgaagtcag gcccgccacg gcctcgtcca gcaagagcag ctttggccct
     4081 gttgcgagtg cccgggccac ttccaggcgt ttcttgtttc cgatggtcag cccgccggcc
     4141 ggccggtccc ggaattctcc caaaaagcag gttttcaggc atttttccgc gatcgcctcc
     4201 gccttttcgg gatccggttc cctcaggtag gctcccacca ggatgttttc caggacggtc
     4261 atctccttca gggggcggac gacctgaaag gtcctggcca tcccgaggcg cgagatccgg
     4321 taggcgggaa ggttggtgat gtctttcccg tcgaaccgga tcgtcccgtc cgtaggcttg
     4381 tagtacccgg caacacagtt gaacagggtg gttttccccg cgccgttggg gccgatgagg
     4441 ccgacgatcg ttccctgttc aacgtcgaag gaaacgtcgc tgttggccac cagggaaccg
     4501 aatttcatcg tcaggttgcg cacttcaagg agggccatct cagttttcct cccttcccgg
     4561 tttccgctgc ccggttttga agccgaggtc ctggaggatt cccatgattc ccttcggttg
     4621 cctgatcatc atgagcatga tgatgatccc gaagatgatc agatcgacgc cgctgcccag
     4681 cgaggtgagg taggccctgc tgtattcctg gagcgggacc aggatcgctg ccccgaggag
     4741 agggccccag aaattgccga caccgcccag gatcgtgatc agcacgaatt tcatcgacat
     4801 gtcgagggac atgaccatgg gaggatcgac ccggtagttg tattgaacga aaaatccccc
     4861 gcaaagcgcg gccaggaagg ctgaaaaggc catggcggac aacttgaccg cggcactgtt
     4921 cactcccagg gattctgcgg tttcctggcc ttcgcggacg gcccggagat agtaacccag
     4981 ccgatgcgtt tcgatccagc ggacgacgcc gaaaatcagg atgaagagcc ctagcgctcc
     5041 gtaatagaaa ccgaccttcg tttccgacca cacaaagttt ttccagccgt cgggaaggat
     5101 ggggtaatcc aggcccaacg ccccgccgac ccaatcccag accatgaaaa ggcggttgaa
     5161 aatctcgacg atggcgaagg ttgcgatggc gaagtaatga cccttgagct tgaagcaagg
     5221 gtagctgatg agaagcgaaa ccagggccgc caggacggcc ccggcgagta gtcccggcca
     5281 cggggtcaga ccgtaggtga cgactgccat tcccgctcca taggccccga tgccgaagaa
     5341 gaccgagtga ccgaaggaaa cctggcccgc gtagccgctg acgatgttcc aggactgggc
     5401 catgctcgcg taaaggagga cgaggatgag aacatgggcg cggaaggaat tcccgatcag
     5461 gcccggtacg agtggaaggg ccgcaaggaa gccaatcagg ccgatccaga tccagtcctt
     5521 cgtttccagc ttgcgtgtct tcatgtttct caccacccga acaggccctt gggccggacc
     5581 agaatgaccg ccaggtagat ggcgaagacc gcaacgtact tgaagaccgg ggcgatgtag
     5641 aacccggcga gggcttccac gaggccgatg atcaccgctg cggcgagggc gcccggaatg
     5701 ctcccaaagc cccctagggc cacgatgaga aaggcgatga gcccgaacag ggaacccacc
     5761 tccgggtgga ccgccaggta ggaggtcaac aggccgccag cgatgccgac acaggcccct
     5821 cccaggccga acaccaggag gtagatgcgg tccgtgttga tgcccatgag ttcagcggct
     5881 tcgcgatcca tggcggtcgc ctggatcgcc catcccaggc gggttttttt catgaggaag
     5941 tacagcgcga aaaggagcaa gagcgaaaag atgcccgtca cgagctgggg catgccgatc
     6001 acgatgtttc ccagcaggat ctttttccct tccaggagag tggactgcag aatccggaag
     6061 ttcggggaaa aacggttaag acagaggttc ttaaggaaca tggaaaggcc gaacgtggcc
     6121 agcagcgggg ccatggcagc cttgccgatg cagcgggaca cgaggaaacg gtacgtcagc
     6181 accccgagga ggaacacgaa gaccccggag gcgacccagg agaaaagggg gtccacgtgg
     6241 gccaaaaacc ctatccagta gctcaggtac atggcgacca tcaggaactc cccgtgggcg
     6301 aaattgatga cgtccatgac gccccagatc aggctcaatc ccgccgcgac aagggcgtag
     6361 agcatcccgg ccagaaggcc gtccagcaaa acctgcgcga gcgtgctcat gcgagatcct
     6421 cctaaagaaa ccggtcagtg aaaaaagggg cggggatcaa accccgcccc ggtctatgcc
     6481 gttcgggaaa ccgctttgcg ggaacgctta gcgcttgtcc cagggggtca tcgggacggt
     6541 ggccttggtc tccgcgtagc tgggcggata aacggggacg tatttcccgt cgtgcagctg
     6601 ggtgatgatg ctggttgcct tcatgttctg gcctccgggt ccgaaggcga ctttcccacc
     6661 gagggaaagg ggagaatcga acgtcgtggt gcggatcact tcggcgacct tgtcgacatc
     6721 cagggttttg gccttttcga tggcctgggc caggatgaag agggcaacgg cttcctggat
     6781 ggaatcgctg tcaaacggga ccccgacctt cttcgaataa atcttctcca cggcagccac
     6841 tttcttcatg tacttcgcgc cgatttccgg cgagtacccc gttcctccca tgaagaagtt
     6901 gccgtctgcg gccagctgct gggcgatctt cggatcctgg tagccggtgc agtagttcag
     6961 ggcaaccttg gggagccagt tcatctgctt catcgtccgg acccaaaggg tatagtcacc
     7021 gccgaggcag gccccgaaaa cggcatccgg gttcttggcc ttcagggtct ggacttcgct
     7081 gttcaggttc gtggcaccgg ggttgaaggc gacgtccgcg acgacctcga agccggcggc
     7141 ctttgccgca gccttgcctt catcggccgc gtgcttcccg aactcggtgt tctcgtagat
     7201 gaggccgatg gtcttgattc ccgccttctg gtttttgttc agccactgga tgtattcgac
     7261 gaattccttg gattcggtct tgtcggtggg ggcgaggcgg aagaagtact tgaagtcccg
     7321 ctcggtcagg gcagcagaac tggagcagcc gcacatgaag agtttcttgg cgcgctcggt
     7381 caccgcggaa gccggtttgg atgcggaact gttgtaggat ccgatgatgg cgtaaacgcc
     7441 ttcctggttc acgagccgct ccgcttctga ttttgcgacg tccggcttgc cctgggtgtc
     7501 ggcgaccacc agctggatct tcatcccctt gaggatgcct tcctgcttgg cgagggggac
     7561 gtccagttcg ggatgcttgt tgttgatgac ctcggcggcg gtttccaccg cggccacgca
     7621 gcgctgtcca gcggtggcgc aggggccggt caagggaaag aggacgccga ttttcaggac
     7681 gttctcggcg gctagggcac tgccggcaga gagaagcagg gcgaagacga gcagcgaaac
     7741 gatggacagg aacttgcgct tttgcatgac ttctatccct ccttaagaat ttggtggcat
     7801 gccccggttt gcggaaaaag cgccccggca agcacgattc gaaatatatc atatttcaac
     7861 cctggcaaaa accgtcgagg ttagcaaaat ctccgctagc aacctaaaat aaaaacattc
     7921 tagttaacga agtccttatt tatgaatatt tgcggaaata tgagcgatcc caccaggttg
     7981 cctggggccg gattcccctt gtacaattcg gttcataaaa gcaaagcaga acccgaatga
     8041 agggattcgg gaaggagcga caggatgccg gaaatcattc aggtttcgga ggccgtatca
     8101 cttgcgcttc atgggctcgg ccttctcgtt tccaggaagg gacgaatcag catccgtgaa
     8161 atggcagcgg agatgggcgt ttcggaggct cacctggcga aggtctttca gagactttca
     8221 aagggggggc tggttcgttc catgcgggga cccggaggag ggttcgtcct ggcaaaagcc
     8281 ccgtccgatg tcacccttta tgaggtctat tcgctgatcg agggcgaacc cgaggtgagg
     8341 atctgcctgc tcgactgcgg aaaatgccct ttcaggaaat gtcttttcga ggggcttctc
     8401 gtgaaaatga agcaggagtt tgtcaatttc ctccgcaaaa agcgtttaag cgacctcgag
     8461 gaatgaaaaa gggcgacgtg ccacggcgcg tcgcccctta gatcctagcc acagccgctg
     8521 ggatccctat tccttttcct ttttcgcgag cctttccagg accagggtga aaccatctgc
     8581 cccgtgctca atgagattct ttgcccggct gatcgtcgcg gtgctggccc ccgtcttctg
     8641 gacgacctgc ggatagttgt tcccctcgta gaggagtcgg gcgacctcga gccgctgggc
     8701 aaaagcaatg atttcaccga ttgtggcgac gtcctccagg aaacgatagg cctcgtcctc
     8761 cgacttgaga gagagaaggg ccttgcagag ctgatcggtc aggcggtctt tccacttttc
     8821 ggacacgctc accaactcct ttggaagtct atcagggatt cgatgcgtaa ttatagcaga
     8881 aaactttaat gcgttcaatc accattaagt gcctgaatcc gtttttggtt aacagtttcc
     8941 ggaccagatc cggcatctcg gacagtagga ccgagcgccc gagagggcat cgcaaagcca
     9001 ttcaccgcat cgggaacaat ggatgggact gagccggggc cgggcagcgg caggttccgg
     9061 catttccaga ggggaagcgt cgggttcgcc ggccggggtt acggttctgt tctttgccgg
     9121 gtgttcctgg gaaaaaaggt ccagctgttt ctgttcggtt ttcattccgt tgctcccttc
     9181 cgcctcaccg ggggttcttc ctgaaaatcc agccggtccg ccagttcctc gatggagcgg
     9241 tgaagccccc gaagctgttc gaaagccttt tggtactccc cgtagagtcg tcgatagtcc
     9301 cctgcaagaa gccggtaccg ctccaccgcc tgggcccttc taacctgggc ttcgagtcgt
     9361 ttcttgcctt gattcgcaag gatctcccgt tccaggcggg tgatccacgc ttgtccgtcc
     9421 tcaagctggg cttggatttc ccgcagccgt ttctcgagtc cggaaacttc gacttcaagc
     9481 catcgggcct cggctgaaag ccgatcgtag gtctgccgcg tctcgcggag ggaaatcgtt
     9541 ttccataatc gaacggcccc gttggcggca aacaggatta aaagagcggc gaccaggggg
     9601 cccaggcggg tctttgccga tcgtgactgg aataaggtca gcctcaccac cccggcagac
     9661 atcataacac cgcaccccgg ccttgtcttc ct
//
LOCUS       DAOOVN010000034         9423 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_4583_length_9423_cov_14.852797, whole genome shotgun sequence.
ACCESSION   DAOOVN010000034 DAOOVN010000000
VERSION     DAOOVN010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 9423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9423
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4583_length_9423_cov_14.852797"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1168
                     /gene="porA"
                     /locus_tag="PLY83_04360"
     CDS             2..1168
                     /gene="porA"
                     /locus_tag="PLY83_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HOV29306.1"
                     /translation="MPRREITSGNWAFAEAMRQINPDVVAAYPITPSTDIPMKFAEFV
                     ANGRVNTNFVTVESEHSAITACVTAAAAGARVMTASSANGVALMHEIFPIAGCFRTPI
                     VFGLVNRSIGAPINIHCDHSDSMAERDAGWIHLYCEDAQEAYDTAILAVRLAEHKDVL
                     TPVFVCQDGFITSHGFEPVNLLDDETVKAFVGERDADFPLLDVDRPVTYGSFVMSEYY
                     FEIKRQQVEAMKNVLRVYDGIAKEYEAVSGRFYPKLDRYRTEDADIVVVVMSSASGVV
                     KDVVDELRDRGIKAGCLRVRMFRPFPAEEVAAALGNAQAVAVLDRALSIGGTAPLAAE
                     VKSALYDAGKTIPVQSCVYGLGGRDFFPQYARELFMDLAAGKVSREERYLGLLE"
     gene            1182..2120
                     /locus_tag="PLY83_04365"
     CDS             1182..2120
                     /locus_tag="PLY83_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOV29307.1"
                     /translation="MAIRIKDYTQKESPLTNGHRMCPGCGAPTAVHQGLMGIESPVVV
                     VNATGCLEVSTTVYPFSSWRVPWIHTAFENAGAGVSGVEAAYVALKRRGKIDKEIKFV
                     AFGGDGGTYDIGLQSLSGALERGHDFTYICYNNGGYMNTGAQRSSATPRGADATTSPA
                     GSVLPGKLQKPKDLTAIAAAHHIPYVAQTTLFNATDVAEKVKRAVETPGPAFVNILVP
                     CVLFWGIDPALQQEICRLAADTRFWPVYEVVDGAWKLTYKPKKRVPIEEFLKPQGRFR
                     HLFRDGKSSALLEQIQADVDADWQAILERSGETWDA"
     gene            complement(2166..3257)
                     /locus_tag="PLY83_04370"
     CDS             complement(2166..3257)
                     /locus_tag="PLY83_04370"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOV29308.1"
                     /translation="MYRIRVLRTLAVAGVLLLAAVCPVPGAGAEPRVPVKPEILVWDH
                     VRSTPPDLLADPPHFAVTVLSPTWFSLGGQDGSVVSKADPAYVREAKKRKVKVEPLFS
                     NGFDPDRTRAFLLDATARARSIAQLLAFCKNLGLDGINVDFENVFDEDRDRLTAYVEE
                     LARALHGNGLRISVDVTVVSDKPNWSACYDRTALGRIADAVVLMAYDEHWRSGPVSGP
                     VASIGWVERSVAELVGFVPPEKIWLGVPFYTREWEESLVNGTWTKTGARALSMPEAEA
                     RLAENGAPLSWDEGAGLYYGEYLKGGKRYRIWLEEERSLALKAALVARYGLGGIAAWR
                     RGFEKPEAWKVLEELRKGLKPVLLGQAGK"
     gene            3519..3959
                     /locus_tag="PLY83_04375"
     CDS             3519..3959
                     /locus_tag="PLY83_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29309.1"
                     /translation="MDLSVERDVLTGLPFEEHTLSLQLLLSPQEKIEAEVFFCDPEYI
                     VANCMTLSSEAVTLWKKTGNPGEPFVPVRPWILHLKDVTEGFVFSSSSPSALDPIERY
                     FRNDWLRNFRREWSTRKSFWTSKPQEFAVECEKARGKIAGIILD"
     gene            complement(3946..6105)
                     /locus_tag="PLY83_04380"
     CDS             complement(3946..6105)
                     /locus_tag="PLY83_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HOV29310.1"
                     /translation="MNEVHIGRIAGELGVRPGQVKAAAELLQDGGTVPFIARYRKEAT
                     GALDEVQVARVRDRLEQLAELDKRREAVMRSLDERGLLTEELKIALEAAETLTRVEDL
                     YLPFRPKRRTRASIAAERGLEPLADLLMASAAGKADCPDPLAAAKMFVDPDKGVPDAE
                     AALAGARDIVAERISEDPSARHALRKLFVRKGVFSSRVVKGKEEEGAKYRDYFDWKEP
                     AVKAPSHRILAAFRGEAEGFLALSFVPPEADALSTLEELFVKGESPAAEQVKAALKDG
                     YARLAGPSMETELRAVLKKKADEAAVAVFARNVREVLMEPPLGQKAVLAVDPGFRTGC
                     KLAVLDPQGALLETAVIYPLRGKNDLENAAKTVTELLARHPVEAVAVGNGTGGRETEA
                     FFRSLDLPGKPLVVLVNESGASVYSASEAAREEFPDQDLTVRGAVSIGRRLQDPLAEL
                     VKIDPKSIGVGQYQHDVDQKLLKSALDDVVSSCVNSVGVEVNTASRQLLTAVSGIGPA
                     LAASIVAYRQEHGPFANRAELKKVPRLGPKAFQQAAGFLRVRGGNEPLDASAVHPESY
                     GVVRAMAKDLGCTVADLMADANLRRRIDPKRYLDGLTGEFTLKDILEELEKPGRDPRQ
                     TFDPVSFSPEVRTIGDLRVGMELAGIVTNVTAFGAFVDIGVHQDGLVHVSEMARGYVK
                     NPAEVVRPGQRVRVRVIAIDESRKRIGLSMKELSSPG"
     gene            6215..7225
                     /gene="ord"
                     /locus_tag="PLY83_04385"
     CDS             6215..7225
                     /gene="ord"
                     /locus_tag="PLY83_04385"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF040740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-diaminopentanoate dehydrogenase"
                     /protein_id="HOV29311.1"
                     /translation="MKPTKIVLWGVGAMGSGIARVIGQKAEMQVVGAIEADPRKVGLP
                     LSDFTGVPEQENVPVSCSLEELPNSEPDLFIVATASRVKDVFQQVMAVLDRGLPVITT
                     AEEMAYPWKAEPGLCAEMDKAAKEKGVAVLGTGINPGFVMDLLPVVLSAACTDVKRIR
                     VTRVNDLSPFGPTVLREQGVGLTAEEFARKVEEGSLEGHVGFLQSIAMISDALGLGVD
                     RIEQFKEPIVSSVRREEKGIVVNPGQVAGCSQTGIGYRKGEPVVELIHPQQIHPHLEG
                     VATGDTVELFGTPDIRLEIRPEIPGGTGTIALAVNSIPLVLKARPGLLTLLDLPLPHA
                     IR"
     gene            7382..8248
                     /gene="lysX"
                     /locus_tag="PLY83_04390"
     CDS             7382..8248
                     /gene="lysX"
                     /locus_tag="PLY83_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysX"
                     /protein_id="HOV29312.1"
                     /translation="MKSATPIRAPRLLIPYSRLRQEEKMLASAAERLGLPVDFRDVAS
                     LFWPRDFDAAARDVAVCRCVGQTHNLAVARLLESRGVKTINSSSVMTLCGDKIATAAR
                     LEAAGIPQPEYAVAASPEAAVEAAEELGYPVVFKPACGSWGRLLAKVSDRPGAEAVAF
                     HKAAMGAAHSVFFIQKYVEKPGSDLRAILLGGQPVSLMRRRSSHWITNTARGGSPEPC
                     EIPSPLADLLRSVARAVGGDFLAVDLFETSCGWLVNEVNGQPEFHGSVEATGLDLAGM
                     LLKYAWNFIPRD"
     gene            8635..8808
                     /gene="lysW"
                     /locus_tag="PLY83_04395"
     CDS             8635..8808
                     /gene="lysW"
                     /locus_tag="PLY83_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019878 - lysine biosynthetic process via
                     aminoadipic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysW"
                     /protein_id="HOV29313.1"
                     /translation="MAGTVTCVVCEAAVALPENCSLGEILVCGDCGTELEVVALDPAR
                     AEEAPQVQEDWGE"
     gene            8801..>9423
                     /locus_tag="PLY83_04400"
     CDS             8801..>9423
                     /locus_tag="PLY83_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HOV29314.1"
                     /translation="MSRLRILYSRLRAEEKQLFAAAESRGIPCSLEDIRTEPWSIGSH
                     DTDVFLARCIGHLQNLSVARMLEAGGATVVNPSSVMELCGDKLATSAALAAAGIPQPA
                     FRAAFSEEAALEAAEQLGYPVVFKPVTGSWGRLLALASNRETAEAIVEHKAHLGPAHQ
                     VYYIQSYVEKGGFDVRAFVVEGRPLCAIRRTSDHWITNTARGGRAEN"
BASE COUNT         1725 a         3131 c         2899 g         1668 t
ORIGIN      
        1 catgccgcgc agggaaatca cgtcgggaaa ctgggccttc gcggaagcca tgcgccagat
       61 caacccggat gtcgtcgcgg cctaccccat caccccgtcc accgacattc ccatgaagtt
      121 cgcggaattc gtggcaaacg gcagggtgaa cacgaacttc gtcaccgttg aaagcgagca
      181 ctcggccatc acggcctgcg tcacggcagc ggccgctggg gcccgggtca tgacggcctc
      241 gagcgccaac ggcgtggccc tcatgcacga gatctttccc attgccggat gcttccggac
      301 ccccatcgtc ttcggcctgg tgaaccggag catcggagcc cccatcaaca tccactgcga
      361 ccacagcgac agcatggccg agcgtgacgc gggctggatc cacctgtact gcgaggacgc
      421 ccaggaagcc tacgacacgg cgatcctggc cgtgcggctg gccgagcaca aggacgtcct
      481 caccccggtc ttcgtctgcc aggacgggtt catcacgagc cacgggttcg agccggtgaa
      541 ccttctcgac gacgaaacgg tgaaggcgtt cgtgggagag cgggacgcgg actttccgct
      601 cctggacgtc gaccgccccg tcacgtacgg gtctttcgtc atgtccgagt attactttga
      661 aatcaagcgg cagcaggtgg aggccatgaa gaacgtcctt cgggtctacg acgggatcgc
      721 gaaggaatac gaagccgtgt cggggcggtt ctacccgaag cttgaccgct accgcacgga
      781 ggacgccgac atcgtcgtcg tggtcatgtc ctcggcctcc ggggtggtga aggacgtcgt
      841 ggacgagctg cgcgaccggg ggatcaaggc cggctgcctg cgggtgcgga tgttccgccc
      901 cttcccggcg gaggaggttg cggccgccct gggcaacgcc caagctgtgg cggttttgga
      961 ccgggccctg agcatcggcg ggacggcccc gctggccgcg gaggtcaaga gcgccctgta
     1021 cgacgccggc aagaccatcc ccgtgcagtc ctgcgtctac ggcctgggcg ggcgggattt
     1081 cttcccccag tacgcccggg agcttttcat ggatcttgcc gctgggaaag tctcccgcga
     1141 agaacgctac ctgggactgc tggaataggg ggggaattga gatggcgatc cggatcaagg
     1201 actacacgca gaaagaaagc cccctgacca acgggcaccg catgtgcccg ggctgcggcg
     1261 cgccaacggc ggtgcaccag gggctcatgg gcatcgaatc gcccgtggtc gtcgtcaacg
     1321 ccacgggctg cctcgaggtt tccaccaccg tttacccgtt cagctcctgg agggtgccgt
     1381 ggatccacac ggccttcgag aacgcggggg ccggggtttc cggcgtcgaa gcagcgtacg
     1441 tggcgctcaa aaggcgcgga aagatcgaca aggaaatcaa gttcgtggcc ttcggcggcg
     1501 acggcggaac ctacgacatc gggctccagt ccctgtcagg ggccctggag cggggtcacg
     1561 acttcacgta catctgctac aacaacggcg ggtacatgaa caccggggcc cagcggagca
     1621 gcgccacccc gaggggggcc gacgccacga cctccccggc gggaagcgtc ctgccgggca
     1681 agctgcagaa acccaaggac ctgaccgcca tcgcggcggc gcaccacatt ccctacgtgg
     1741 cccagacgac cctgttcaac gcaacggacg tggcggagaa agtgaagcgg gccgtcgaaa
     1801 cgccgggacc ggcctttgtg aacatcctcg tgccctgcgt cctgttctgg ggaatcgacc
     1861 cggccctgca gcaggaaatc tgcaggctcg ccgcggacac ccgtttctgg cccgtctacg
     1921 aggtcgtcga cggggcgtgg aaactcacgt acaagccgaa gaagcgcgtt cccatcgagg
     1981 agttcctcaa gccccagggg cgcttccggc acctgttcag ggacgggaaa tccagcgccc
     2041 tgctggagca gatccaggcc gacgtggatg ccgactggca ggccatcctc gaacggtccg
     2101 gcgaaacctg ggacgcctga aaagctcatg gccgcgggga gccggaaatt gaacggctcc
     2161 ccgcgctatt tcccggcctg ccccaaaagg accggcttca ggcctttccg gagttcttcc
     2221 agcaccttcc aggcctcggg cttttcgaag cccctccgcc aggccgcgat gcccccaagc
     2281 ccataccggg ccacgagcgc cgccttcagg gccagcgagc gttcctcttc cagccagatc
     2341 cggtaccgct tcccgccttt caggtactcc ccgtaataga gacccgcccc ttcgtcccag
     2401 gaaaggggcg ccccgttttc cgcgaggcgg gcttccgcct ccggcatcga aagggcccgg
     2461 gctcccgtct ttgtccaggt tccgttcacc aggctttctt cccattcccg cgtgtaaaaa
     2521 gggaccccaa gccaaatctt ctccggcgga acgaagccga ccaactcggc gacggagcgc
     2581 tccacccagc cgatcgaagc cacggggccg cttaccggac cgctcctcca gtgttcgtcg
     2641 taagccatca ggaccaccgc gtcggcgatc ctgcccaggg ccgttcggtc gtagcaggcc
     2701 gaccagttcg gcttgtcgga aaccaccgtc acgtcgaccg agatcctcag gccgtttccg
     2761 tgaagggcgc gggcaagctc ttccacgtag gccgtcagtc ggtcgcgatc ctcgtcgaac
     2821 acgttttcga aatcgacgtt gatcccgtca agccccaggt tcttgcagaa ggccaaaagc
     2881 tgcgcgatcg aacgggccct ggcggtagcg tccaggagaa aagcccgggt ccgatccgga
     2941 tcaaacccgt tggaaaaaag aggctcgacc ttcaccttcc gttttttcgc ctcgcggacg
     3001 taggccgggt ccgccttcga gaccaccgac ccgtcctgcc cgcccaggga aaaccacgtc
     3061 gggctgagga cggtcacggc aaagtggggc ggatcggcta aaaggtccgg gggggtcgaa
     3121 cggacgtgat cccataccag gatctccggt ttgacgggaa cgcgcggttc agccccagcc
     3181 cccggaacgg ggcagaccgc tgccaggagc agcacaccgg cgacggccag cgtccgaaga
     3241 accctaatcc tgtacacact aaaaacccct tgggtttcca cggatttttc ccctttccct
     3301 tttgatgatg gggcaaagaa cacaaggcat cttcaaaaag tttcacatcc cggttttttt
     3361 gaacgaggag acttacaatt taaaattgtt atatttaaaa aaccaatctt ttaggtacct
     3421 ttgccttgcg ttaataaaac atttcggtat aataagttta ttcctggccc cattccagga
     3481 tcttcatatt gacttccccc cgaaaggagc ggatgcgatt ggacctttca gtggaaagag
     3541 atgtcttgac gggcctccct ttcgaggagc acaccctttc cctgcaattg ctcttaagcc
     3601 cccaggagaa gatcgaggcc gaggttttct tctgcgaccc ggaatacatc gtcgcgaact
     3661 gcatgacgct gtccagcgaa gcggtcacgc tgtggaagaa gacaggaaat cccggggaac
     3721 cgtttgtccc cgtgcgtccc tggattctcc acctgaagga cgtgaccgaa ggattcgtct
     3781 tctcttcctc gtccccatcg gccctggatc ccatcgaacg ctacttccgg aacgactggc
     3841 tgcggaactt ccgaagggag tggtccaccc gcaagtcctt ctggacctcg aaaccccagg
     3901 aattcgccgt ggaatgcgaa aaggcccggg ggaaaatcgc cgggatcatc ctggactaga
     3961 aagctccttc atcgaaagcc cgatgcgttt cctggactcg tcgatggcga tgacccgaac
     4021 gcggaccctc tgacccggcc ggacgacctc ggccgggttt ttcacgtagc ctcgggccat
     4081 ttcgctcacg tggacgagcc cgtcctggtg gaccccgatg tccacgaaag ccccgaaggc
     4141 cgtcacgttg gtcacgatcc cggccagttc catgcccacc cgaagatccc cgatcgtcct
     4201 gacttccggc gaaaacgaaa cgggatcaaa cgtctgccgc ggatcccgcc ccggcttttc
     4261 cagttcctcg aggatgtctt tcagcgtgaa ttccccggtc aggccgtcca ggtaccgttt
     4321 cggatcgatc cgcctcctca ggttcgcatc ggccatcagg tcggcaacgg tgcagcccag
     4381 gtctttcgcc atcgccctca cgaccccgta ggattccggg tggacggcgc tggcgtccaa
     4441 aggttcgttt cctccccgga cccgcaggaa cccggccgcc tgctggaagg ctttcggccc
     4501 cagcctgggg acctttttca gttcggcccg gttggcgaag ggcccgtgct cctgccggta
     4561 ggcgacgatg gatgccgcca gcgccgggcc gatccccgaa accgcggtca acagctgccg
     4621 gctggcggta ttcacctcga cgcccacgct gttcacgcac gagctcacca cgtcatccag
     4681 ggcgctcttc aacagtttct ggtccacgtc gtgctggtac tgtcccaccc cgatggattt
     4741 cgggtcgatc ttcaccagct ccgccagggg atcctgcagc cggcggccga tcgaaaccgc
     4801 ccccctgacg gtcaggtcct ggtcggggaa ctcctcgcgg gccgcctcgg aggccgagta
     4861 gaccgaggcc ccgctctcgt tcaccagcac gacaaggggc ttcccgggaa gatccaggct
     4921 ccgaaagaag gcctccgttt cgcggccgcc cgtgccgttg cccaccgcca cggcttccac
     4981 cgggtgccgg gcaagcagtt ccgtcaccgt tttggccgcg ttctccaggt cgttctttcc
     5041 tcgaaggggg taaatcacgg ccgtttccag cagggctccc tgcgggtcca gcacggcgag
     5101 cttgcagccg gtccggaacc cgggatccac cgccagcacc gccttctgcc ccagcggagg
     5161 ttccatcagg acctcccgga cgttccgggc gaacaccgcc acggccgcct cgtcggcctt
     5221 ttttttcagg accgcccgaa gctccgtctc catcgacggc ccggccaggc gggcgtaccc
     5281 gtctttcagc gccgctttca cctgctcggc ggcaggggat tcgcctttga cgaaaagctc
     5341 ctcgagcgta gacaacgcgt ccgcctcggg ggggacaaac gaaagcgcga gaaatccttc
     5401 cgcctctccg cggaaggccg cgaggatccg gtgggagggg gccttcacgg cgggctcctt
     5461 ccagtcgaaa tagtcgcgat acttggcccc ttcttcctcc tttcccttca ccacccggct
     5521 ggaaaacacc cccttccgga caaaaagctt cctcagggcg tgccgggctg aaggatcttc
     5581 cgagatccgt tccgccacga tatccctggc accggccagg gccgcttcag cgtccgggac
     5641 acccttgtcc gggtccacga acatcttggc ggctgccaga ggatcggggc agtcggcctt
     5701 tcccgcggca gaagccatca gcagatcggc caggggctcc aggccgcgtt ccgcggcgat
     5761 cgaggcccgg gtccggcgct tcggccggaa gggcaggtac aggtcttcga cccgcgtcag
     5821 cgtttctgcc gcctccaggg cgatcttcag ctcttcggtc aacaggccgc gctcgtccag
     5881 cgacctcatc accgcctcgc ggcgtttatc cagctccgcc agctgttcca gccggtcccg
     5941 cacccgtgcg acctgcacct cgtccagggc cccggtggct tcctttcggt accgggcaat
     6001 gaaaggaacg gttcccccgt cctggagcag ttccgccgcc gccttgacct gcccgggcct
     6061 gacccccagt tcaccggcga tccgcccgat atggacctcg ttcatcgaaa aacctccctg
     6121 ctggcgcttc ctgctttctt tggctatcct acaccaagac ccccaccccg cggcggggtc
     6181 agaccggagc ccaacggctg gaggaggcca ggaaatgaag cccacgaaaa tcgtcctttg
     6241 gggagtaggc gccatgggaa gcggcatcgc ccgggtaatc gggcagaaag cggaaatgca
     6301 ggtcgtcgga gccatcgagg ccgacccccg gaaggtcggc ctgcccctct ccgatttcac
     6361 cggggttccc gaacaggaaa acgtccccgt ttcctgctcg ttggaagaac tgccgaattc
     6421 ggaaccggac ctcttcatcg tggccaccgc ctcgcgggtg aaagacgtct ttcagcaggt
     6481 catggccgtc ctcgaccggg ggttgcccgt catcaccacg gccgaagaga tggcctatcc
     6541 ctggaaggct gagccgggcc tttgcgcgga aatggacaag gcagcgaaag agaagggggt
     6601 tgccgtgctc ggaacgggga tcaatcccgg tttcgtcatg gatttgcttc ccgtcgttct
     6661 ttccgcggcc tgtacggacg tgaagcggat ccgcgtaacc cgcgtcaacg acctctcgcc
     6721 cttcggcccg acggtcctgc gggaacaggg agtcgggctc accgccgaag aattcgcccg
     6781 gaaggtggag gaaggctccc ttgaaggcca cgtgggcttc ctccagtcca tcgccatgat
     6841 ttcggatgcg ctcgggctcg gcgtcgaccg tatcgagcaa ttcaaagaac cgatcgtttc
     6901 ctcggtccgg cgggaggaaa agggaatcgt cgtcaacccc ggacaggtgg ccggatgcag
     6961 ccagaccggc atcggctacc ggaaaggtga gcccgtcgtg gaactcatcc acccgcagca
     7021 gattcacccg cacctcgaag gcgttgccac aggcgacacg gtcgagcttt tcggaacgcc
     7081 cgacatccgg ctcgagatcc ggccggaaat ccccggcggc accggaacca tcgccctggc
     7141 cgtcaactcc atccccctcg tcctgaaggc ccgccccggg cttttgaccc tgctggacct
     7201 tccccttccc cacgccatcc gctaacccca cgctgggaat gaaccgggcg aaggggccgg
     7261 tttgttcctg gccttgcaag tgaacaaaaa gaatatttag ttttagtatt tgacatagaa
     7321 taaactctgg tttatgatcc cggagtcctg aacgaacccg actccgagga ggtttgaccc
     7381 gatgaagtcc gcgaccccga tccgcgcacc gaggctcttg attccctaca gccgtttgcg
     7441 gcaggaggag aagatgcttg catcggcggc ggaaagactg ggacttccgg tggatttccg
     7501 cgatgtcgcc agcctcttct ggccgcggga ttttgacgcg gccgccaggg acgtcgcggt
     7561 ctgccgctgc gtgggacaga cccataacct tgcggttgcc cgcctcctgg aatcgcgggg
     7621 cgtgaagacg atcaactcct cttccgtcat gaccctttgc ggggacaaga tcgctacggc
     7681 cgcccgcctg gaggcagccg ggatcccgca gccggaatac gccgtggccg cctcgccgga
     7741 agccgccgtt gaagcggcgg aagagctggg atacccggtg gttttcaaac cggcctgcgg
     7801 cagctggggc cgcctcctgg cgaaggtctc cgatcgcccc ggcgcggagg cggtcgcttt
     7861 ccacaaggcg gcgatggggg ccgctcactc cgtgttcttc atccagaaat acgtggaaaa
     7921 acccggttcc gatctccggg cgatcctcct gggcggccag cccgtttccc tgatgcggcg
     7981 ccggagctct cactggatca cgaacacggc caggggaggg tcgccggaac cctgcgagat
     8041 tccctccccc ctggccgacc tgcttcggag cgtggcgagg gccgtcggcg gggatttcct
     8101 ggcggtcgac ctgttcgaaa cttcttgcgg ctggctcgtg aacgaggtca acggccagcc
     8161 ggagtttcac ggctcggtgg aagccacggg gttggacctg gccggcatgc ttctgaaata
     8221 cgcctggaat ttcatcccca gggattgaca agtcgggccc tgccgtctaa actccatttc
     8281 aattcgcgat gagcgatctg gtacggacca cttttccaat cgaaatcttg aacaaatgcg
     8341 atgacgcgag tggcctgacg gggcaacagc cctacagcga gggacctcca ccggatgaga
     8401 ggggtgccgg gctgccgaaa ggcccaaggc tcgaagagca ggcgccgaaa gggaaccttc
     8461 ccgtagtagg ttgcccggga acggacagcc gttacctgtc cgagagcggg accgcccccc
     8521 ggtcggggga cgcgtccaag ttgggtggta ccgcgggtct agcacaggac tcgtcccttc
     8581 acgatgtgga ggggcgggtc ctgtttttta tcccactcga aggaggagtt tgaaatggca
     8641 ggaacagtga cgtgcgtggt gtgtgaagca gcggtggcgc tcccggagaa ctgcagcctc
     8701 ggagagatcc tggtctgcgg cgattgcggg acggagctgg aagtggtggc ccttgacccg
     8761 gcccgggccg aggaagcccc gcaggtgcag gaggactggg gtgagtaggc tccgcatcct
     8821 ttactccagg ctccgggcag aggaaaagca gctgttcgcc gctgccgagt cgagggggat
     8881 tccctgcagc ctggaggaca tccggaccga gccctggagc atcgggtccc atgacacgga
     8941 cgtgttcctc gcccgctgca tcggccattt gcagaacctc tcggtcgccc ggatgctcga
     9001 agccggggga gccacggtcg tgaacccgag ttcggtcatg gagctttgcg gggacaagct
     9061 ggccaccagc gctgccctgg ctgccgccgg catcccccag ccggcctttc gcgcggcctt
     9121 ttccgaggaa gcggcattgg aagcggccga gcagctgggg tatcccgttg tcttcaagcc
     9181 cgtcaccggc agctgggggc gcctgctcgc cctggcctcc aaccgggaaa ccgccgaggc
     9241 gatcgtggag cacaaggctc acctgggacc cgcccaccag gtctattaca tccagtccta
     9301 cgtcgagaaa ggcgggttcg acgtccgggc cttcgtggtc gaaggacgtc ctctctgcgc
     9361 gatccggcgt accagcgacc actggatcac caacaccgcc aggggaggca gggcggaaaa
     9421 cca
//
LOCUS       DAOOVN010000035         9340 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_4615_length_9340_cov_15.447496, whole genome shotgun sequence.
ACCESSION   DAOOVN010000035 DAOOVN010000000
VERSION     DAOOVN010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 9340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9340
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4615_length_9340_cov_15.447496"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2273)
                     /locus_tag="PLY83_04405"
     CDS             complement(<1..2273)
                     /locus_tag="PLY83_04405"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOV29315.1"
                     /translation="MSDPCGSLGKSYDPRPIEDKWYAWWLEKGLFEGNPGSEKPPFSI
                     VIPPPNVTGSLHMGHALNNTLQDIQCRYKRMNGYDVLWLPGTDHAGIATQNVVERNLA
                     SQGISRHDLGREAFVEKVWEWKEQYGNRIVNQLKKLGASCDWRRERFTMDEGLSRAVR
                     AVFVRLYKEGLIYKGKYIINWCPRCQTALSDLEVEHQESGGNLYHVLYRFPDAEGGVT
                     VATTRPETILGDVAIAIHPRDEKNRHLVGRVVLVPLVGREIPIIEDPMVDPEFGTGCV
                     KITPAHDPNDFLVGQRHGLESIQVIDSEGRMNEAAGKYFGMDRFEARKAVLKDLDSQG
                     CLLRVEEHLHSVGHCYRCRTIVEPYLSEQWFVRAKPLAEAGVEAVRAGRIRFVPEQWT
                     GTYYQWMEEIRDWCISRQLWWGHRIPAWTCSVCGHITVSEADPDVCESCGSKDIHQDE
                     DVLDTWFSSALWPFSTLGWPDPTPELSRYYPTSLLVTGFDIIFFWVARMIMMGLHFMG
                     EVPFRDTYIHALVRDESGQKMSKSKGNVIDPLDIIEEYGADALRLTLAALTVQGRDIL
                     LSTNRIETFRFFLNKLWNASRLALSSLEGKTRGREIDSLSLRLHDRWILTRLDEVILE
                     TTRDLEDYFFGEAAKLLYDFVWGELCDWYLEMAKPALRGEEGESRKEATQAVLCQVFE
                     SVLKLLHPFIPFLTEELWHAFRFSDRSLQEEEWPKPGRKEAIPTVCLEEMSAFQEAVR
                     AIRNLRAEARLNPQQTAP"
     gene            complement(2276..2881)
                     /gene="yihA"
                     /locus_tag="PLY83_04410"
     CDS             complement(2276..2881)
                     /gene="yihA"
                     /locus_tag="PLY83_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOV29316.1"
                     /translation="MPLQWRYEEAITAFLENQLPPPGLPEITILGRSNVGKSSLINKL
                     AGRSIARSSSTPGKTRSINFFRLRGKHSFYLVDLPGYGYSAVSQDIRRTWKRLIDAYM
                     ARNSAILHLHLVDFRHGYLKGDEALKLWLASLEAPTVTVFTKADKIARGRWKTLFSNY
                     FKGQEPAFITSAETGAGVDDLRHFLEDYVDRSLGGDGHEEE"
     gene            complement(2881..5229)
                     /gene="lon"
                     /locus_tag="PLY83_04415"
     CDS             complement(2881..5229)
                     /gene="lon"
                     /locus_tag="PLY83_04415"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOV29317.1"
                     /translation="MRRISPRRKLPVVPLRDTVLFPGMVLPLFIGRPRSIHAVESASS
                     KGKRVVLVAQKNSREEDPSEEDLYELGTLASVLQTATLPDGTLKVLLEGLSRVQIEAF
                     DVSDERIEAWVLPVPPQNEDVEDQPLRRALLHQFETIASYSGKISDEITGSLHEIESL
                     SMLADVITSHVELKVEEKQRILETADPVAKSETLLKFLIRETEILQLESEIQERVRQE
                     IDRTHREYYLKEQMKVIQTELDSRQGLNEAEELAGKIRKAGMPAEVEEKALQELNRFS
                     KMPPVSAEATVARSYIEWLIELPWSKTSRDRLELGLAKTVLDADHYGLDEVKERILEF
                     LAVRKLASKDGRSQVLCFVGPPGVGKTSLGRSIARAMDRKFVQMSLGGIRDEAEIRGH
                     RRTYVGALPGRILQKIRQAGTRNPVLLLDEIDKVGADFRGDPSAALLEVLDPEQNSAF
                     TDHFLEVPFNLGQTLFITTANVTHTIPAPLLDRMEIIRIPGYVTEEKVQIAQRHLWPK
                     VLKSSGLDRQPVTIESETLRMIVERYTSESGVRELERHLFRIARKLARRILETGGPVK
                     GKRKQTQIRIDPVDLTEYLGAPRKSHPSLPEHDEVGAAVGLAWTESGGEALVVESVTM
                     KGKGALILTGNLGEIMQESATAAMGYLRSTAGKWGLDEVDWQSLDTHVHVPEGAIPKD
                     GPSAGITIATALLSCLSRRAIRHDTAMTGEITLRGKVLPVGGIKEKVLAAKRHGIRSL
                     ILPAGNRADWEELPEWVREEMSVTFVSSADDVFGLALKGETS"
     gene            complement(5233..6516)
                     /gene="clpX"
                     /locus_tag="PLY83_04420"
     CDS             complement(5233..6516)
                     /gene="clpX"
                     /locus_tag="PLY83_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOV29318.1"
                     /translation="MFQQDDGNGRKRKNLRCSFCGKPQDEVNRLISGPGVTICNECVH
                     LCNIILKDEMEELHQEDPGLPFSIGKLPNPREIFRFLDNYVVGQTLAKKILSVAVYNH
                     YRRIQNNAAGQDDVEMQKSNVVLIGPTGSGKTLLAQSMARFLKVPFAMADATTLTEAG
                     YVGEDVENILVRLLQAADYDLKAAERGIIYLDEVDKIGRKSESASITRDVSGEGVQQA
                     LLRILEGTVANVPPKGGRKHPYQDFIQMDTRNILFICGGSFEGLEEIIANRINKKTIG
                     FGGTVLSNDKRARYELLSHVQPEDLVTFGFIPEFVGRLAIFAPLEELDEESLVRILRE
                     PKNALIRQYEKAFELEGVRLHCTDEAVRSIARLAAKRNTGARGLRSILESIMLDLMFD
                     LPSRSAEIASVTITRETVEEGAPPLIEPKSGKEVA"
     gene            complement(6652..7236)
                     /gene="clpP"
                     /locus_tag="PLY83_04425"
     CDS             complement(6652..7236)
                     /gene="clpP"
                     /locus_tag="PLY83_04425"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOV29319.1"
                     /translation="MSILVPMVIEQTGRGERSYDIYSRLLKDRIVFIGQEIDDHVANI
                     VIAQLLFLESEDPDKDIYLYINSPGGIVTAGLAIYDTMQYVKCPVSTICVGQASSMAA
                     VLLAAGEKDKRIALPNARIMIHQPLGGAQGQATDIDIQAREILRVKQTVNGILAKHTG
                     QSIEKITLDTDRDFFMSAEEAKTYGIIDRIIEKR"
     gene            complement(7251..>8063)
                     /locus_tag="PLY83_04430"
     CDS             complement(7251..>8063)
                     /locus_tag="PLY83_04430"
                     /inference="COORDINATES: protein motif:HMM:NF017509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29320.1"
                     /translation="TTIQLDPETLREELKNALTGKNVGDEESVEITIEAEHPDPSLAG
                     KTLRYNLKIKEVLERILPELDEAFIRKTSQDRLANLEEFRREVAERIEEQIRRDVQNA
                     MEANALSKLREEAQVTVPETLIERQVASMKEDEAAQIKKQFNLSLEEYLDKNGITPEA
                     FEKGIRERAERIVRQTLILESLADERSIEVLPQDIDKEIRNLADSLHADPERIRSLLF
                     KDRERMTDFLHRIRMNKAISQLLSEVTIKEVRPPSGTEGDATSSAAGGKDPE"
     assembly_gap    8064..8163
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8529..8613)
                     /locus_tag="PLY83_04435"
     tRNA            complement(8529..8613)
                     /locus_tag="PLY83_04435"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8577..8579),aa:Leu,seq:tag)
     gene            complement(8625..8700)
                     /locus_tag="PLY83_04440"
     tRNA            complement(8625..8700)
                     /locus_tag="PLY83_04440"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8665..8667),aa:Lys,seq:ctt)
     gene            complement(8733..8808)
                     /locus_tag="PLY83_04445"
     tRNA            complement(8733..8808)
                     /locus_tag="PLY83_04445"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8773..8775),aa:Arg,seq:tcg)
     gene            complement(8826..8902)
                     /locus_tag="PLY83_04450"
     tRNA            complement(8826..8902)
                     /locus_tag="PLY83_04450"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8866..8868),aa:His,seq:gtg)
     gene            complement(8930..9006)
                     /locus_tag="PLY83_04455"
     tRNA            complement(8930..9006)
                     /locus_tag="PLY83_04455"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8970..8972),aa:Arg,seq:tct)
     gene            complement(9018..9094)
                     /locus_tag="PLY83_04460"
     tRNA            complement(9018..9094)
                     /locus_tag="PLY83_04460"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9058..9060),aa:Gly,seq:tcc)
     gene            complement(9116..9193)
                     /locus_tag="PLY83_04465"
     tRNA            complement(9116..9193)
                     /locus_tag="PLY83_04465"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9156..9158),aa:Pro,seq:tgg)
     gene            complement(9230..9304)
                     /locus_tag="PLY83_04470"
     tRNA            complement(9230..9304)
                     /locus_tag="PLY83_04470"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9270..9272),aa:Ala,seq:cgc)
BASE COUNT         1698 a         2683 c         2725 g         2134 t
ORIGIN      
        1 ggagccgttt gctgggggtt cagacgggct tccgcccgga gattccggat cgcgcggacc
       61 gcttcctgga aggcggacat ctcttccagg caaaccgtgg gaattgcttc ttttctgcct
      121 ggcttcggcc attcctcttc ctgcagggaa cggtcagaga aacggaaagc gtgccaaagc
      181 tcttccgtca ggaaggggat aaagggatgc agcaacttga gaacggactc gaacacctgg
      241 caaaggactg cctgcgttgc ctccttgcga ctttcccctt cttctcccct cagggcgggt
      301 ttggccatct cgaggtacca gtcgcagagt tctccccaga cgaaatcgta cagtagcttg
      361 gcggcttccc cgaagaagta gtcctccagg tcacgggtgg tttcaaggat gacttcgtcg
      421 agtcgggtca ggatccacct gtcatgaagg cgtagagaga gagaatcgat ttcgcggccc
      481 cgtgtcttcc cttcgaggga cgacagagca agccggctcg cgttccagag cttgttgagg
      541 aaaaaccgga aggtttcgat tcggttcgtg ctcagcagga tgtccctgcc ctggacggtc
      601 agtgcggcga gagtcagccg gagggcgtcg gcgccgtatt cttcgatgat gtccaggggg
      661 tcgatcacgt tccccttcga tttgctcatt ttctggccgg actcgtcccg gacaagggca
      721 tggatgtacg tatccctaaa ggggacttct cccataaagt gcaatcccat catgatcatc
      781 ctcgcgaccc agaagaagat gatatcgaat ccggtcacga ggagacttgt gggatagtag
      841 cgggaaagct ccggcgtcgg atccggccat ccgagggtgg agaagggcca gagtgcgctg
      901 ctgaaccagg tgtcgaggac gtcctcgtct tggtggatgt cctttgagcc gcaggattcg
      961 caaacgtcgg gatccgcttc agaaacggtg atatgtccgc aaaccgaaca ggtccaggcc
     1021 ggaatgcggt gtccccacca tagctgccgt gagatgcacc agtcccggat ctcttccatc
     1081 cactggtagt aggtgcccgt ccattgctcg ggaacgaagc ggatccgtcc ggccctgacg
     1141 gcttccacac cggcttctgc gagcggtttt gcccggacga accattgttc cgaaaggtag
     1201 ggctcaacga tggttcggca ccggtagcag tgaccgaccg agtgaaggtg ctcttcgacc
     1261 cgcagcaggc acccctggga atccaaatct ttcagaacgg cctttctggc ttcgaaccgg
     1321 tccattccga agtatttccc ggcggcttcg ttcattcggc cttcggaatc gatcacctgg
     1381 atcgactcaa ggccgtgcct ctgcccaacg agaaagtcgt tcggatcgtg ggcgggggtg
     1441 attttgacgc agcccgtgcc gaattcggga tccaccatcg gatcctcgat gattggaatt
     1501 tcccttccga cgagaggtac gaggacaacc cgcccaacca ggtggcgatt cttctcgtcg
     1561 cgcgggtgga tcgcgatggc gacgtccccg aggatggttt ccggtctggt ggtggcaacg
     1621 gtgacgcccc cttctgcatc ggggaagcgg taaagaacat ggtagaggtt tccgccggat
     1681 tcctggtgtt cgacttccag atccgagagg gcggtctgac agcgcgggca ccagttgatg
     1741 atgtatttgc ccttatagat caggccttcc ttgtaaaggc ggacgaaaac ggcacggact
     1801 gccctggata gcccttcgtc catcgtgaac ctctcccggc gccagtcgca ggaggcaccg
     1861 agcttcttca gctggttcac gatccggttc ccgtactgct ctttccattc ccacaccttt
     1921 tcgacgaaag cctcgcgtcc gaggtcatgg cgggaaatcc cctggctcgc gaggttgcgc
     1981 tcgacgacgt tttgagttgc gatgccggcg tggtcggtcc ccggaagcca caggacgtcg
     2041 tacccgttca tccgtttata gcggcactgg atgtcctgca gggtattgtt cagtgcgtgc
     2101 cccatgtgga gcgatcccgt cacgttgggc ggcgggatga cgatgctgaa agggggcttt
     2161 tccgagccgg ggttgccttc gaagaggcct ttctcgagcc accaggcata ccacttgtcc
     2221 tcgatgggcc tgggatcgta ggattttcca agactgccgc agggatcact catcgtcatt
     2281 cctcctcgtg accgtcgcct ccgagcgacc ggtccacgta atcttccaga aaatgccgaa
     2341 ggtcgtcaac accggccccg gtttcggccg acgtgataaa agcaggttcc tgccctttga
     2401 agtaattgga aaagagcgtt ttccacctgc ctctggcgat cttgtcggcc ttggtgaaga
     2461 ccgtgactgt cggtgcttcc agagaagcaa gccaaagctt cagggcctca tcccccttga
     2521 ggtagccatg acggaagtcc accaggtgaa ggtgcagaat cgccgagttc ctggccatat
     2581 aggcgtcgat cagtcgtttc caggtgcggc ggatatcctg ggaaacagcg gaatatccat
     2641 agccgggaag gtcaaccagg tagaacgaat gtttcccgcg gagccggaaa aagttgatgc
     2701 tccgcgtctt gcccggagtc gagctcgaac gggcgatcga tcttcccgcc agcttgttga
     2761 tcaggctgga tttgccgaca ttcgatcttc cgaggatcgt gatctcgggc agtcctggcg
     2821 gaggcaactg gttttcgaga aaggccgtga tggcctcttc gtagcgccat tgcaggggca
     2881 tcaggaggtt tctcccttga gggcaagccc gaagacgtcg tcggcggaag agacgaacgt
     2941 gacggacatc tcttcacgga cccattcggg cagttcctcc caatcggccc ggttgccggc
     3001 agggaggatc aagctccgga tcccgtgacg tttcgcagcg agcacttttt ccttgatgcc
     3061 gccgacggga aggaccttgc cgcgcagggt gatctcgccg gtcattgccg tgtcgtggcg
     3121 gatcgccctc ctgctgaggc atgaaagcag tgcggtggca atggtgattc ctgcagaagg
     3181 tccatccttg gggatcgccc cttcggggac gtgaacgtgg gtgtccaggc tttgccagtc
     3241 gacctcgtcg agtccccact ttcctgcggt ggagcgaagg tagcccatgg cggccgttgc
     3301 ggattcctgc atgatctcgc cgaggtttcc cgtgagaatg agcgccccct tgcccttcat
     3361 cgtgacactt tcaacgacga gggcttcgcc tccgctctcg gtccaggcca gtccaacagc
     3421 tgctcccacc tcgtcgtgct caggcaggga aggatgactt ttcctgggag cccctagata
     3481 ttccgtcagg tccacgggat cgatccggat ctgggtctgc ttcctcttcc ctttcaccgg
     3541 gcctccggtc tcaaggattc gtcgtgcgag cttgcgggcg atgcggaaaa ggtgacgttc
     3601 gagttcccgg accccggatt cgctggtgta gcgctccacg atcatccgca gggtttcgga
     3661 ttcgattgtt acgggctgtc gatccaggcc gctggatttc agcactttcg gccaaaggtg
     3721 gcgctgtgca atctggacct tctcttccgt cacatagccc ggtatccgga tgatttccat
     3781 ccggtcaagg agcggggcag ggatcgtgtg ggtcacgttt gccgtcgtga tgaaaagtgt
     3841 ctggccgagg ttgaagggca cctcgaggaa atggtccgtg aaagcggaat tctgttcggg
     3901 atcgagcact tcaagcaggg ccgctgaagg gtcgccccgg aagtcggcac ccaccttgtc
     3961 gatctcgtcc aggagaagca cgggattccg ggtgcccgcc tgtcggatct tttgcaggat
     4021 cctgccagga agggctccca cgtaggttcg ccggtgtccc cgaatctccg cctcgtcccg
     4081 aattccacca agggacattt ggacgaactt tcggtccatc gcccgtgcga tggatcgtcc
     4141 cagggaggtc tttccgactc ccggtgggcc cacgaagcag aggacctgcg aacggccatc
     4201 cttcgaggcc agtttcctga ctgcaaggaa ttccaggatc ctttccttga cttcgtccaa
     4261 cccgtaatga tccgcgtcca gaacggtttt cgcgaggccg agctccagcc ggtccctgga
     4321 ggtcttgctc cagggaagct cgatcagcca ttcgatgtag gaccgggcaa ccgtcgcctc
     4381 agccgaaaca ggaggcatct tggagaaacg gttgagctcc tggagggcct tctcctccac
     4441 ttcggcgggc atcccggcct tccggatctt gcctgccagt tcttcggcct cgttcaaccc
     4501 ttggcgggag tccagttccg tctggatcac tttcatttgt tctttcaggt agtactcgcg
     4561 atgtgtccgg tcgatctcct ggcgtacgcg ctcctggatc tcgctctcca gctggaggat
     4621 ctccgtttcc cgaatgagga acttcagcaa ggtctccgat ttggccacgg gatctgccgt
     4681 ttccaggatc cgctgttttt cttccacttt cagttccaca tgggaggtga tcacatccgc
     4741 cagcatggaa aggctctcga tctcgtggag cgatccggtg atttcgtcgg agatcttccc
     4801 gctgtaggag gcaatcgttt caaactggtg caggagagcc ctccgcagtg gctggtcttc
     4861 tacgtcttca ttctgaggag ggacgggaag cacccacgcc tcgatcctct cgtccgaaac
     4921 gtcgaaggct tcgatctgca cgcgggacaa gccttccagc agcaccttga gggttccgtc
     4981 cgggagcgtg gctgtctgga ggacggaggc cagcgttccc agttcgtaca ggtcttcttc
     5041 ggaaggatcc tcttcccttg agtttttctg ggcaaccaac acgacgcgtt tccctttcga
     5101 cgaggcggac tcaacggcat ggatggatct tggcctcccg atgaacagcg ggagcaccat
     5161 ccctggaaaa agaaccgtgt cccggagagg gacaaccggc aatttccgcc gaggagaaat
     5221 tcgcctcatg gatcaggcaa cctccttgcc gctcttgggt tcgatcaacg ggggggcgcc
     5281 ctcctcgaca gtctccctcg tgatcgtcac gcttgcaatt tcggcggatc ttgagggaag
     5341 gtcaaacatc aggtccagca tgatcgattc cagaatcgac cgtaatcccc tggcccccgt
     5401 gttccgcttc gccgcaaggc gggcgatgct ccggaccgcc tcgtccgtgc aatgaagccg
     5461 gactccctcg agctcgaagg ccttttcata ttgccggatc aaggcgttct tgggttctcg
     5521 caggatacga accagggatt cttcgtcaag ttcctccagc ggagcaaaga tcgccagcct
     5581 gccgacaaac tccggaatga acccgaaggt gacgagatct tcgggctgga cgtgggagag
     5641 gagctcgtac cgggcccgct tgtcgttgct caggactgtt ccgccgaaac cgatggtttt
     5701 cttgttgatc cggttggcga tgatttcctc caggccttcg aaggagcccc cgcagatgaa
     5761 gaggatgttc cgggtgtcca tctggatgaa atcctggtag gggtgtttcc ggccaccctt
     5821 gggagggacg ttggcgacgg ttccttcgag gatccgtaga agggcctgct gaaccccctc
     5881 cccggaaacg tcgcgggtga tggaggcgga ctccgatttt ctcccgatct tatcgacctc
     5941 gtcgaggtag atgatccccc gctcggcggc cttcaggtcg taatcggcgg cctgaaggag
     6001 gcggaccagg atgttttcca cgtcctcgcc cacgtacccg gcttcggtca acgtcgtggc
     6061 gtcagccatg gcgaagggaa ccttcaggaa tcgggccatg ctctgggcaa gcagggtttt
     6121 tccgcttccg gtggggccga tcagaacgac gttgctcttt tgcatctcga cgtcgtcctg
     6181 tcccgcggcg ttgttctgaa tccgtcggta atggttgtag accgcgaccg agaggatctt
     6241 ttttgccagg gtctgtccca cgacgtagtt atccaggaaa cggaagattt cccggggatt
     6301 cgggagtttc ccaatggaaa acgggagtcc gggatcctcc tggtggagtt cctccatttc
     6361 atccttcaga atgatgttgc atagatggac gcattcgttg caaatcgtga ccccgggtcc
     6421 tgaaatcagc cggttcacct cgtcctgcgg ctttccgcag aaggagcatc ggaggttttt
     6481 ccgttttcgg ccgttgccgt catcttgctg gaacatgatg gtcctcccct cttcagcctc
     6541 agttttcctg aagccgggaa caacttcata gtctatcgca tgccggaaaa aagcagaagg
     6601 ggagaggcgc tcctctcccc ttccctgggg tccggcttcg gttttgccgt cctagcgctt
     6661 ctcgatgatg cggtcgatga ttccgtaggt tttcgcttcc tcggccgaca tgaagaaatc
     6721 gcgatcggtg tcgagcgtga tcttttcgat ggattgcccc gtgtgcttcg ccagaatccc
     6781 gttgaccgtc tgtttcaccc ggaggatttc cctggcctgg atatcgatat ccgtggcctg
     6841 tccctgggcg cctcccagag gctggtggat catgatccgg gcgttgggaa gggcgatccg
     6901 cttgtctttc tccccggcgg cgagcagcac ggctgccatg ctgctcgctt gccccacgca
     6961 tatggtcgat acggggcact tgacgtactg catggtgtca tagatggcaa ggccggccgt
     7021 gacgattcct cccggactgt tgatgtacag gtagatgtcc ttgtccgggt cctcgctttc
     7081 aaggaaaagc agctgggcga tgacgatgtt tgcaacgtga tcgtcgatct cctggccgat
     7141 gaaaacgatc ctgtccttca ggaggcggct gtagatgtca taggaacgct ctcctctgcc
     7201 ggtttgctcg atgaccatcg ggaccagaat ggacatgttt ggacctcccg tcattcagga
     7261 tcttttccgc ccgcggcgga agaggttgcg tccccttccg tccccgaggg agggcgcact
     7321 tccttgatcg tgacttccga aaggagctgg ctgatggcct tgttcatgcg gattcggtgg
     7381 agaaagtcgg tcatgcgctc acgatccttg aaaagcaggg agcgtatccg ctctggatcg
     7441 gcgtggagag aatctgccag gtttcggatc tccttgtcga tatcctgggg gaggacctcg
     7501 atcgaccgct cgtcagccag cgattcaagg atcagcgtct gccggacgat ccgctcggct
     7561 ctttcccgga tgcccttctc gaaggcctcc ggggtgattc cgttcttgtc gagatactct
     7621 tccaaggaaa gattgaactg cttcttgatc tgtgcggctt cgtcctcctt catggacgcg
     7681 acctgccgtt cgatcagcgt ttcagggacc gtcacctggg cctcttcgcg cagctttgaa
     7741 agggcattcg cctccatggc gttctgcacg tcccggcgga tctgttcttc gattctctcg
     7801 gccacttccc tgcggaattc ctcgaggttt gccagccgat cctgcgaggt cttccggatg
     7861 aaagcctcgt ctagctcggg gagaattcgc tcgaggacct ccttgatttt caaattgtac
     7921 cggagcgttt ttccggcaag ggaaggatcg ggatgttccg cttcaatggt gatttcgacg
     7981 gactcctcgt cgccgacgtt ctttccggtc agggcgtttt tcagctcctc ccgcagggtt
     8041 tccgggtcaa gctgaatcgt cgtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnncgtccga agtacatttc caggacattg cggggaactt tcccttttct gaaacctttg
     8221 atgttggcct tgcgggacag ctcgccgatg accgtgttca gagctttttg aaactcctgg
     8281 gcatcgatcg tcgcccggat tcgctgtttg ttgccctctt gtgaaatgat ctcggttttc
     8341 aaccgccaca actcctttca ggttccattc atttaatgtc gggatcaggc gtgaagagta
     8401 tatcacatgg tttggggtcc gaagcacaga agaaaagaac cccccggatc cccttggaag
     8461 tcggtgggtt ctctttgaca gcacacccgg tgcagtttgg acaaggcgtt cccgggatgg
     8521 aacttccctg gtgcgagagg cgggacttga acccgcaagg ctttcgccac tggatcctaa
     8581 gtccagcgtg tctaccggtt ccaccactct cgccggaaaa aagatggtgg accgtacagg
     8641 aatcgaacct gtaaccccct gattaagagt caggtgctct gccagttgag ctaacggtcc
     8701 acagcaactc gatatttgat tgttgtattg gctggcgcgc ccggcaggat ttgaacccgc
     8761 gaccaacgga tccgaagtcc gccgctctgt ccactgagct acgggcgcat gtcccgcctt
     8821 caatttgggg tgagcgaggg gacttgaacc cccaaccact ggagccacag tccagtgctc
     8881 tgaccaattg agctacgctc accggctcca atccctcatt gttcccaagt ggcgcgcctg
     8941 agaggacttg aacctccaac ctacggatta gaagtccgtt gctctatccg gttgagctac
     9001 aggcgcctgt atttctttgg agcgggaaac gggattcgaa cccgcgacct acggcttgga
     9061 aggccatcgc tctagccagc tgagctattc ccgcagtttc cgcttagctt cagggtggtc
     9121 ggggcgaaag gatttgaacc ttcgacaccc tgctcccaaa gcaggtgcgc tgaccaggct
     9181 gcgctacgcc ccgacattct ctatgattac agggtcaaaa tcaatgtcct ggtggagctg
     9241 aggggattcg aacccctggc ctattccgtg cgaaggaatc gcgctcccac tgcgccacag
     9301 ccccgctcaa tgcgacgtcg accattttag agagggcggg
//
LOCUS       DAOOVN010000036         8933 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_4850_length_8933_cov_14.632688, whole genome shotgun sequence.
ACCESSION   DAOOVN010000036 DAOOVN010000000
VERSION     DAOOVN010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8933
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4850_length_8933_cov_14.632688"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(235..1413)
                     /locus_tag="PLY83_04475"
     CDS             complement(235..1413)
                     /locus_tag="PLY83_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOV29321.1"
                     /translation="MEQERSVYLNNAATSWPKPKCVASAMADFLEGRGANLARGSASR
                     RDLSTLDMVTTCRIKISALFGGPDADPRNVTFAANVTEALNIVLKGFLKPGMRAATSS
                     VEHNAVMRPLRRLEREGVSVAVMPCTAEGVLEPDTLARELEKGLDLVVLTHASNVCGT
                     VQDLDLLAPLCEAAGVPLVLDTAQTAGVLPIDAARWNLAACCFTGHKGLLGPQGTGGI
                     LWREDFAKECRPLIEGGTGSFSHEEVQPSVLPDKFEAGTPNLPGLAGLLASLNWLEEK
                     EVETVRRKEEVLGKRLLDGLLEIQGITLYGKQTMEGRLSVFALNLENLDNGRAALELA
                     DRWGIETRPGLHCSPAAHKTLGSFPQGALRISPGFFNTDEEIDRCLEALRILSREHRR
                     "
     gene            complement(1439..2455)
                     /gene="selD"
                     /locus_tag="PLY83_04480"
     CDS             complement(1439..2455)
                     /gene="selD"
                     /locus_tag="PLY83_04480"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(2435..2437),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOV29322.1"
                     /translation="MARTSGUAAKIGPADLEKLLQSLKPDSGGRLLTGWDHGEDAALW
                     AIDENRVGILTLDFITPIVDDPELWGQIAAANALSDVFAMGGKPFVALNIVGFPVHCE
                     PLELLEAVLKGGQSKVIEAGAVLAGGHSVEDEEPKYGLAVFGEAEKAKIWRVSGARPG
                     DILILTKPLGTGLCTTAIKAGMADLLDTRPVFDSMARLNDLPRHLDAGLHLAVKACTD
                     VTGFGLAGHLLDMLSGGRIDCLLDPEALPAFKGVLAAADMGLIPAGTYRNRELYSSRI
                     DNLEKAPLSLQDLIFDPQTSGGLLLAVAPEAAQAFLELCQSGCAPESRAIGRFLDGTG
                     RIRF"
     gene            complement(2515..2736)
                     /locus_tag="PLY83_04485"
     CDS             complement(2515..2736)
                     /locus_tag="PLY83_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HOV29323.1"
                     /translation="MTDKILVDARGLSCPQPVLETKAALESAGQGTVEVLVDTVTSRE
                     NVLRLASRMNWKGTWEETGQGGFRLTLEK"
     gene            complement(2751..3833)
                     /gene="yedE"
                     /locus_tag="PLY83_04490"
     CDS             complement(2751..3833)
                     /gene="yedE"
                     /locus_tag="PLY83_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YedE family putative selenium transporter"
                     /protein_id="HOV29324.1"
                     /translation="MSRFGPSLTGAVVGILAPVLVFLGNPGNMGICVACFSRDIAGAL
                     GMHRAAVVQYLRPEIAGFILGSFVSALVYREYRPRAGSAPIIRFFLGVFAMVGALIFL
                     GCPWRAYLRLSGGDLNALWGIAGLAAGVGIGIAFLWNGFNLGRSAPTANASGLVMPAF
                     SILLLVLLLLAPQFGRDASGLPTGPIFFSKEGPGSMHAPAFIALGAGLLIGWMAQRSR
                     FCTVGALRDLFMLKDPHLFYGILALLVAAVGTNVALGQFKLGFTGQPVAHTNALWNFL
                     GMLLSGLAFTLAGGCPGRQLIMSGEGDNDAALFVMGLIVGAAVAHNFSFASSAKGPGP
                     FALTAFAVGMIFCLAVGFLNRGRVSA"
     gene            4244..5365
                     /locus_tag="PLY83_04495"
     CDS             4244..5365
                     /locus_tag="PLY83_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOV29325.1"
                     /translation="MDAFLAAFAALTAKQAVMLGIGGLLIYLAIAKEYEPALLLPMGF
                     GAILVNIPLSAAVNHVFDGKLQEGVLSLLFNMGIATELFPLLIFLAVGAMIDFTPLFL
                     NPLMFLFGLTAQAGIFLTMGLALVLGFDVREAASIGIIGAADGPTSIYVASKFAPHLL
                     GPISVAAYSYMALVPLIQPPVIRALTTKAERRIPMAYSEREVSRALKIGFPIAVTVIS
                     GFFAPASVALIGFLMFGNLIRESGVVDRLSKGAQNELANIVTLLLGLAISTTLAAERF
                     LQPTTLVIIAMGLVAFVLDTAVGVLSAKLLNLFRKDKVNPMVGAAGISAFPMSARVIQ
                     KLGQKEDPQNFLLMHAVSANVSGQIGSVLAGGLLLALLA"
     gene            5380..6942
                     /locus_tag="PLY83_04500"
     CDS             5380..6942
                     /locus_tag="PLY83_04500"
                     /inference="COORDINATES: protein motif:HMM:NF019520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HOV29326.1"
                     /translation="MQVIDLYKMNLFPDFRLLAGSGGVLNPITTITVFDSPDIHKWLR
                     GGEFLIGNAYIFKDHPEELEHFVRRIREHGASALGIKLDRFLSELPKRIIDVADEIDL
                     PLILIPFSYRWADIIEVVQRHLNAPQGPSPDKGTEIRFLEDFLEPTELACALARELKH
                     SVYIYCPSLDLQLFVSEEGTALSLEEANAYFEGEVLVEDQLPAHGTIQVTRTTRNIPP
                     ETVSVVYRSTGQARTEVHLILKPEEKVPSLRQERLAMVVLNLIRSFALEKILGNQVSA
                     GSQAAFLEKLCLGGFSDQKVALQRASALGLKVPEPCFVTILQLADSRDSLEKGEEESF
                     VFHIGHLRVSITPWPEREPHFERISGFCQKAKIWGVVGRKAEHLIEIQDSYLDCKKSI
                     AILQKTFLPPGAYPHEEAVLFSLFRKIAKSGEGQVLVERYWQPLLDLPEENRTLKPVQ
                     VIETLVKNGFNAKKSAMDLHVHYNTIRNYISEIQALIGLDFDKPMDVLMLTICYLLAH
                     GQGEGRRLFSNA"
     gene            6964..7575
                     /locus_tag="PLY83_04505"
     CDS             6964..7575
                     /locus_tag="PLY83_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29327.1"
                     /translation="MKKKTLVAVIGLVLGICFSFSPAAASLKTADLRNDPGGFRGIEW
                     GKPLSSFEKMVPYKVTPYTNEFYHWRIDGFGYADRFFDDDWKKPLIDGKRENFIREGE
                     SLFFHKVPLIRIVYSFSNGKLSEVTLLFKGDENYLAMEKLTEQLFGPTTVLKCPPVLG
                     AVGRLQTDRFWQGKKTWIQLRWDHLLFTDKTFGFLQFRSRANL"
     gene            complement(7644..8879)
                     /locus_tag="PLY83_04510"
     CDS             complement(7644..8879)
                     /locus_tag="PLY83_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HOV29328.1"
                     /translation="MQVSIDRIRKDIETICSFNATPGKGYTRFSYSAEDLKAREYLLS
                     QFRDLGMECRTDPAGNLRARLEGSSPHAPAVMSGSHIDTVLHGGMFDGLVGTVGALEV
                     ARVLREEGVRPVHPFEVVVFAEEEGSNFGTTTAGSKAMVGRYGVEDLKKLKNPEGTSM
                     YEAAKSIGLDPDKLPESILKPGDLKAMVELHIEQSVVLDTEELQIGIVEAIAGIRAYS
                     LSLEGVPNHAGATPMTLRQDPLAGAAEIILEAERAARECPTGSAVGTVGRIFCEPNVS
                     NVIPGKVRFTLDARDVKTQGMDEVVEKVRSRMEEVAAKRKLSAEMKLVGESRAIEIGR
                     EMTELIESVAMKLGARYRRMNSGAVHDACLIADVAPIGMIFVPSINGRSHVPEENTRW
                     SDIEMGANVLLEALLKLLF"
BASE COUNT         1728 a         2668 c         2725 g         1812 t
ORIGIN      
        1 agaaaaacga aagcagggat ccccccaaaa gacccctgct ctcgtttaga ttctttcatg
       61 aggcaacgaa cacccttcgt ttagattctt aggtgaggca acatggactg tttagcactt
      121 gaaacgaaac tgaaccagag atgtcaggat ttcaggatcc tgaagccggg caggacccca
      181 ttgctgtcgc gaatcgcatc ccggatgccg gttcccggta aacccggggc agcttcatcg
      241 gcgatgttca cggcttagga tccggagggc ttccaggcag cgatcgatct cctcgtcggt
      301 gttgaagaaa ccgggtgaaa tccggagggc cccctggggg aacgatccca gggtcttgtg
      361 ggcggccggg gaacagtgga ggccggggcg ggtttcgatg ccccagcggt cggccagttc
      421 cagggcggcc cggccgttgt ccaggttctc gaggttcaag gcaaagacgg aaaggcgtcc
      481 ctccatggtt tgtttcccgt aaagcgtgat cccctggatt tcgagaagcc cgtccaggag
      541 cctctttcca agcacttcct ccttccggcg gacggtttcc acttcctttt cctccagcca
      601 gttcagcgag gccaaaagcc ccgcgagtcc gggcaagttc ggcgttccgg cctcaaattt
      661 gtctggaagg acagaaggct gaacctcctc gtgggaaaag ctgccggtcc ccccttcgat
      721 cagggggcgg cattccttcg caaaatcctc cctccagagg atcccgccgg ttccctgggg
      781 ccccagcaag cccttgtgtc ccgtgaagca gcaggcggcc aggttccagc gggcggcgtc
      841 gatgggcaga acacctgccg tctgggccgt gtccaggacc agcgggaccc cggccgcttc
      901 gcagagcggg gcaagaaggt ccaggtcctg cacggttccg cagacgttgc tggcatgagt
      961 cagaacaacg aggtccagtc ccttctccag ttcacgggca agggtgtcgg gttcgaggac
     1021 gccttccgcc gtgcagggca tcacggcgac cgaaacccct tcgcgttcca ggcgccggag
     1081 cggccgcatg acggcattgt gctccaccga ggaggttgcg gcacgcatgc cgggtttcag
     1141 aaagcctttg aggacgatat tcagggcttc ggtcacgttg gccgcaaagg tgacatttcg
     1201 gggatcggcg tcggggccgc cgaaaagggc ggagattttg atgcggcagg tcgtgaccat
     1261 gtcgagggta ctgaggtccc gccgggaagc cgagccacgt gcgaggttgg ctccgcgccc
     1321 ttccaggaag tcggccatgg ctgacgccac gcatttcggt ttcggccacg aggtggccgc
     1381 gttgttcagg taaacagagc gttcctgttc catgattcct cctttcggaa ggccggcttc
     1441 agaaacggat tctcccggtt ccgtccagga aacgtccgat cgcccggctt tccggcgcgc
     1501 acccgctttg gcagagctcc aggaaggcct gtgccgcttc gggggcgacg gccagcagca
     1561 gccctcccga ggtctgggga tcgaagatca ggtcctggag gctcaggggg gccttctcca
     1621 gattgtcaat ccgggaggaa tagagttccc ggtttcggta ggtcccggca ggaatcaatc
     1681 ccatgtcggc cgcagccagg acccccttga aggccggcag ggcctcggga tcgagaaggc
     1741 agtcgatccg gccgccggaa agcatgtcga gaaggtgccc cgcaaggccg aatcccgtga
     1801 cgtcggtgca ggccttcacc gcaaggtgaa ggcctgcgtc caggtggcgg ggcaagtcgt
     1861 tcagccgggc catggaatcg aacaccggtc tcgtgtccag caggtccgcc atgccggcct
     1921 tgattgccgt ggtgcagaga ccggtcccca ggggcttggt caggatcagg atgtccccgg
     1981 gccgagcccc tgacacgcgc cagatttttg ccttttcggc ttcgccgaac accgccaggc
     2041 cgtatttggg ttcctcgtct tccacgctgt ggccgccggc caggacggct cccgcttcga
     2101 tgaccttcga ttgcccgccc tttagcacgg cttcgagcag ttcgaggggt tcgcagtgga
     2161 ccgggaaccc cacgatgttg agggcgacaa aaggcttccc tcccatggcg aagacgtcgc
     2221 tcagggcgtt ggccgcggcg atttgtcccc aaagttcagg gtcatccacg atgggggtga
     2281 tgaaatccag cgtcaggatc ccgacccggt tttcgtcgat ggcccacaac gctgcatcct
     2341 ccccgtggtc ccaaccagtc agcaagcgtc ccccggagtc gggtttcaag gattgaagca
     2401 gtttttccag gtccgccgga ccgatcttgg ccgctcagcc gctggtccgt gccatctccg
     2461 tcagccttct cacggaggat cgcctccttt ttccggtgtt gctggtttag gttcctactt
     2521 ttccagggtg agcctgaagc ccccctggcc ggtttcttcc caggtcccct tccagttcat
     2581 ccggcttgcc agcctcagca cgttttccct ggacgtgacc gtgtcgacca gcacctcgac
     2641 cgttccctgc cccgcggatt ccagggcggc cttcgtttcc aggacgggct gggggcagga
     2701 aagccccctc gcgtccacga gtatcttatc cgtcatctcc ggatccctcc ctaggcggac
     2761 acgcgtccgc ggttcaaaaa gccgacggcc aggcagaaga tcatcccgac ggcaaaggcc
     2821 gtcaatgcga agggcccggg gcccttcgcg ctgctggcga agctgaaatt gtgggccacg
     2881 gcggccccga cgatcaggcc catgacgaag agagccgcat cgttgtctcc ctcgccgctc
     2941 atgatgagct ggcgccccgg gcagccgccc gccagggtga aggccaatcc cgagagaagc
     3001 atcccgagaa agttccagag ggcgttcgtg tgggccacgg gttgtcccgt gaaaccgagc
     3061 ttgaactggc cgagagcgac gttggtcccg acggcggcga cgaggagggc caggatcccg
     3121 tagaacaggt gcgggtcctt cagcatgaag aggtcccgga gggctcccac cgtgcagaag
     3181 cggcttcgct gggccatcca tccgatcaaa agcccagcgc ccagggcgat gaaagctgga
     3241 gcgtgcatgg agccggggcc ttccttcgag aagaagatgg ggccggtggg aaggcccgac
     3301 gcgtcccgcc caaactgggg ggccaaaaga agcagcacga ggaggagaat ggagaaagcg
     3361 ggcatgacca gccccgatgc attcgcggtg ggggcgctcc ggcccaggtt gaagccgttc
     3421 cagaggaagg cgatgccgat gccgacgccc gcggcaaggc ccgcgatgcc ccagagagcg
     3481 ttcaggtccc cgccggaaag gcgcaagtag gccctccacg ggcagcccag gaagatcagg
     3541 gcgccgacca tggcgaagac gcccaggaag aagcggatga tgggggccga cccggctcgc
     3601 ggcctgtact cccggtagac cagcgcggag acgaaactgc cgaggatgaa gcccgcgatc
     3661 tccggcctca ggtactggac gacggctgcc cggtgcattc cgagtgcgcc ggcgatgtct
     3721 cggctgaagc aggcgacaca aatccccatg ttcccgggat tgcccaggaa cacgaggacg
     3781 ggggcgagaa tccccacgac cgctccggtc agtgacgggc cgaagcggga catcaggaaa
     3841 cggttgatca aggcgaagca cctccctgga atggtgtgcc gcaagaaaag ggagcatgcc
     3901 gcaacagacc gcgggcggtt gagttttcag gccggatggg gtgcgggggt cttcagggaa
     3961 ggaagcaaag gggggcaacg ggacaccgca ccgacgtgcg cgcgccgcgc gcactatcga
     4021 tccggcttca ccggcaggcg atcctgcgga tcatgcgatg ccctcccctc gaggcgaatc
     4081 aagaattgcc ctcattctag ggggactccg gaaaacgtgt caaggcggaa gcgatcttct
     4141 ctctccctct cgaggcgttc tgtgggataa tcatttcttg tgcagctttc aaatccggga
     4201 agagcaggat cgacccggta gacgatcagg ggggacacaa cgaatggatg cttttctagc
     4261 ggcttttgcc gccctgaccg cgaaacaggc ggtcatgctt ggaatcgggg ggcttctgat
     4321 ctacctcgcc atcgcgaagg aatatgaacc cgccctgctg ttgcccatgg gattcggcgc
     4381 catcctggtg aacatcccgc tttccgccgc tgtcaaccac gtgttcgacg gcaagctgca
     4441 ggaaggggtc ctgagcctcc tcttcaacat ggggatcgcc acggaacttt tcccgctgct
     4501 gatctttttg gcagtgggag ccatgatcga cttcacgccg cttttcctga accccctcat
     4561 gttcctcttc ggcctgacgg cccaggccgg gatcttcctg acgatgggac tggccctcgt
     4621 tctcggtttt gacgtccgcg aggcggcttc catcggcatc atcggagcgg cggacggccc
     4681 cacgtccatc tacgtcgcgt cgaagttcgc gccgcacctc ctgggcccga tctccgtggc
     4741 ggcctattcc tacatggcgc tggtcccgct gatccagccg cccgtgatcc gggccctcac
     4801 gacgaaggcc gagcgccgga tccccatggc ctacagcgag cgggaagttt ccagggcgct
     4861 aaagatcggc tttcccatcg cggttaccgt gatctccggc tttttcgccc cggcctcggt
     4921 ggccctcatc ggttttctga tgttcggcaa cctcatccgc gaaagcggcg tcgtggaccg
     4981 gctttcaaag ggtgcccaga acgaactggc caacatcgtc actctcctcc tgggacttgc
     5041 catttcgacc accctggcgg ccgagcgttt ccttcagccc acaaccctgg tcatcattgc
     5101 catggggctt gtggccttcg tcctggatac ggcggtaggg gtgctctccg ccaagctgct
     5161 gaatctcttc cggaaagaca aggtcaaccc catggtcgga gcggccggga tttccgcctt
     5221 tcccatgtct gcgagggtga tccagaagct tggacagaag gaggatcccc agaatttcct
     5281 gctgatgcac gcggtcagcg ccaacgtctc gggacagatc gggtccgtcc tggccggggg
     5341 gcttcttctg gcacttctgg catgaagcga gggaaccgga tgcaggtgat tgatctctac
     5401 aaaatgaacc ttttcccgga cttccgtctt cttgcgggaa gcggaggggt cctgaacccg
     5461 atcaccacca tcacggtttt tgattccccg gatattcaca agtggctcag gggaggggaa
     5521 ttcctcatag ggaacgccta tattttcaag gatcacccgg aggaactgga gcatttcgtc
     5581 cgaaggatcc gggaacatgg agcgtccgcc ctggggatca agctcgacag gttcctgtcg
     5641 gagcttccga agcgaatcat cgatgttgct gacgagatcg accttcccct cattctgatc
     5701 ccgttttcct atcgttgggc cgatatcatc gaagtggtcc agaggcacct caatgccccg
     5761 caaggcccga gcccggacaa agggacagaa atccgcttcc tggaagattt cctcgagccg
     5821 accgaactgg cttgcgctct tgcccgagaa ttgaagcatt ccgtgtacat ctattgtcct
     5881 tcgctggacc ttcagctttt tgtgtctgaa gaaggaacgg cgctctctct cgaggaggca
     5941 aacgcctatt tcgagggaga ggtcctggtg gaagaccagt tgcccgctca cggaaccatc
     6001 caggtgacga gaacgacgag aaacatccct cccgaaacgg tgagcgtcgt ataccgctca
     6061 accgggcagg cgagaacgga ggttcacctg atcctgaagc ccgaggaaaa ggtcccttcc
     6121 ttgaggcagg agcgccttgc catggttgtg ctgaacctga ttcgatcctt tgcactggaa
     6181 aaaatcctgg ggaaccaggt ttccgcggga tcgcaggcgg ccttcctgga aaagctgtgc
     6241 ctgggcggtt tttcggacca aaaagtcgcc ttgcagcggg cctcggccct tggcctgaaa
     6301 gttcccgaac cctgctttgt cacgatcctt caactggcgg attcaaggga ttccctggaa
     6361 aagggggagg aagaatcgtt cgtgtttcac atcgggcact tgagggtttc catcacccca
     6421 tggccggaaa gggaaccgca cttcgagaga atttccgggt tctgccagaa ggcgaagatc
     6481 tggggggttg tcggaagaaa ggccgagcac ctgatcgaaa ttcaggattc ctacctggac
     6541 tgcaagaagt ccatcgcgat tctacaaaag acctttctgc cgccgggtgc ctacccccac
     6601 gaggaagcgg tgttgttttc ccttttccgg aaaattgcca agtcggggga agggcaggtc
     6661 ctggtcgaac ggtactggca gccgctcctg gatctgccgg aagaaaacag gaccctgaag
     6721 cccgtccagg tgatcgagac cctggtgaag aacggcttca atgcaaaaaa gtctgccatg
     6781 gaccttcatg tgcattacaa cacgattcga aactacattt ccgagatcca ggccctcatc
     6841 gggctggatt ttgataagcc gatggatgtt ttgatgctga cgatctgtta tcttcttgcc
     6901 cacggacagg gggaagggcg gaggttgttc tcgaatgcct aggatgacgg gagagggatc
     6961 gaagtgaaga agaaaaccct ggttgcggtg attggactgg ttctggggat ctgtttcagt
     7021 ttcagcccgg cagcggcctc gctgaaaacg gctgatttga gaaacgaccc tggcggtttc
     7081 cggggaattg aatggggaaa gccgctttcg agtttcgaga agatggtccc ctacaaggtg
     7141 acgccgtaca ccaatgaatt ctatcattgg agaatcgacg ggttcggcta tgcggaccgt
     7201 ttctttgacg atgactggaa aaagcctctg attgacggaa aacgggagaa tttcattcgg
     7261 gagggcgagt cgctgttttt ccataaggtt cccctgattc ggatcgttta ttcgttctcg
     7321 aatgggaagc tttccgaggt gaccctgctt ttcaaggggg acgaaaatta cctggcgatg
     7381 gaaaaattga cagaacaact gttcggccca acgactgtcc tgaaatgtcc ccccgttctg
     7441 ggggccgtcg gccgcttgca gaccgaccgc ttctggcagg gaaagaagac ctggatccag
     7501 ctgcgctggg atcatcttct ctttacggat aagacgtttg gattcctgca attccgatca
     7561 agggcaaatt tataggggga aaaagaggaa ggaggacgat ttccgccttc ctcttttcgt
     7621 taggggagcg ccggagcgat acgctagaaa aggagcttca ggagagcttc cagaaggaca
     7681 ttggccccca tttcgatgtc agaccagcga gtattttcct ccggaacgtg gcttctcccg
     7741 ttgatgctgg gaacaaaaat catgccgatg ggggcgacgt ccgcaatcag gcaggcatcg
     7801 tgtacggctc cgctgttcat cctgcggtaa cgggctccca gtttcatagc gacgctttca
     7861 atgagttccg tcatttccct gccgatctcg atggcccggg attccccgac cagcttcatt
     7921 tcagcggaaa gcttccgttt tgcggcaacc tcttccatcc tggaccggac tttttcgacc
     7981 acctcgtcca tcccctgcgt cttgacatcc ctcgcatcga gagtgaaccg gacttttccg
     8041 gggatgacgt tggacacgtt gggttcgcag aaaatccggc cgacggtacc gaccgcgctt
     8101 cccgtcgggc attcccgtgc ggctctttcg gcttcgagga tgatttccgc tgcgccagcg
     8161 agcgggtcct gccggagggt catgggggtg gcccccgcat ggtttggtac gccttccagg
     8221 cttaaggaat aggcgcgaat tccggcaatc gcctccacga tgccgatctg cagctcttcg
     8281 gtatcaagga ccacgctttg ctcgatgtgg agttccacca tggccttcag atccccgggt
     8341 ttcaggatgg attccgggag tttgtccggg tcaagcccga tggattttgc cgcctcgtac
     8401 atggaggtcc cttcggggtt tttcagcttc ttgaggtcct cgactccgta cctgccgacc
     8461 atggccttgc ttcccgcggt ggtcgtgccg aaattcgaac cttcctcctc ggcaaaaacc
     8521 acgacctcga agggatgaac gggccggacc ccttcttccc ggagcacccg ggcaacctcg
     8581 agagcgccga cggttccaac cagcccgtcg aacatcccac cgtgcagcac ggtgtcgatg
     8641 tgggaaccgc tcataacggc cggggcgtgc ggggaactcc cttccagcct tgctcgtagg
     8701 ttcccggcag ggtcggtccg gcactccatt cccaggtccc tgaactggga aagaaggtat
     8761 tcccgtgcct tcaggtcttc ggcagaatag gaaaacctcg tgtagccctt tcccggtgtc
     8821 gcgttgaaac tgcagatcgt ttcgatgtct ttccggatcc gatcgatgga aacctgcatt
     8881 gttgagctcc ctccctttcc actcaccgtg agattaccgt tctttttccg gcc
//
LOCUS       DAOOVN010000037         8393 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_5192_length_8393_cov_14.372631, whole genome shotgun sequence.
ACCESSION   DAOOVN010000037 DAOOVN010000000
VERSION     DAOOVN010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8393
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5192_length_8393_cov_14.372631"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3759
                     /locus_tag="PLY83_04515"
     CDS             <1..3759
                     /locus_tag="PLY83_04515"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HOV29329.1"
                     /translation="LFSFRGMAERLEALLQIPLERPSWFLQTGAIGAAACAWSQDNRT
                     RLDFEQVEAALASFSSGQVFAPPLSDSLRRVFLVRRSPAEQENKPVSRRLVDAALGVD
                     GGSTTTKAALVDAESHTVLDSLYVPTHGDPEGALKTVLRHLARNADQVRILAVCTTGS
                     ARKLYERILTSPSRRAALEAEGFFVPDGTVDEITCHARGVKEFDPEVDTIFEIGGQDM
                     KFTTFKKREGRATTEVQEARMNYSCQAGAGQTLENMAALLGLDVKTTLQEAALAARRV
                     PVIDATCGVFMEMDEKRLVAEGFPVEEIAAAIVRATVAGYFHKFVGGSRTVGSHCSCQ
                     GGPSLGRAVLAALSQVADREIFAYPGRELFGAIGAALCALDAVSEATAAGRKTASAFR
                     GWQVTESPLESGTVLCSSLHGADSCGLRDCKLRTFRIEGETIVTGGFCPRGNSEATGT
                     LKPDYVEVYHRLLERHFTGTAAVRGGAVRAFDRPTVGIKRSGETLGEWGIWCAALLDS
                     LGFHPVLSPVSDEGIVDRGIQLARTDLCFAMKLSLGHSDYLANTEQVDFLFHPAFIHR
                     SEGEGIPRLKYCVYTEGEGFLATETLGLPPERVLAPVWKFGDPYGMAESLQAELGRIG
                     RKTPMGRILEAFEKADAAREAFLEALAAVGETFLKRVADSGQPGYVGLGREYVICDPM
                     ASSGTGSLFSRIRGMNYIPMVFLWKAYRNVPVHELVENEYWFNNAQILKASVFVARTS
                     GLFPIRQMNFACGPDSIKFFMEREIFERADKPFLHLVTDAQTNSAPFATRAEAHERIV
                     AQSRPRPIQTWVELLPPLPKAPKGNARTWILPSMGHVNRLGGALLRHWGIQAAAIPTA
                     GQSAREWARRTIPVETCYPLKGVTGDVLAFLEAESARIGRQAVLDRYAVFLPRASGPC
                     RLGKYDEVLRHCLAQAGFGDLPVLGPHIGKNYRDLPFLPDRARGNPAALFRFVREALH
                     AVLFSDFLDDLCLRLRPYAPDTGRFEEGVESVLETTTAVIEQEGLSETVLRKRIRESK
                     KTLASLSRPDHPRFPFVLFIGEIYMRLHDPYTDHVIRLLESEGLEIIRSGIGEWLDYT
                     RSATRRDGPNLANLLAAAALSFSRSRFVQLGGVPFPERARLPEPAEAIEALERERIFS
                     GEIGGESPINIGIFNEFMKDRLPSIAGLPVCGIFHCGPFTCMHETLAASLMTALAKNW
                     RASHPDALLPILHASFGDSPNPNLEAEIAAFRDQCHARARLESAAPHS"
     gene            complement(3750..4979)
                     /locus_tag="PLY83_04520"
     CDS             complement(3750..4979)
                     /locus_tag="PLY83_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOV29330.1"
                     /translation="MIAFGEILHVSLRSLRANKMRSALTTLGIVIGVAAVVAMFAIGT
                     GASRNVEAQLASMGTNLLVVRSGSTTSGGARTGWGGSPTLTFADADAIVKECPAVGKA
                     APTAAGSVQIVYGNSNWSTRAEGTTPPMLDIRNWTIGSGRSFNTSEVRSAAKVCLLGQ
                     TVVDNLFGGEDPIGKVVRVKKIPMVVVGVLGKKGTNAMGQDQDDTLIIPITTAQKRVF
                     GSRFQGKVSSILVQAADSNSISAAIEQIDALLTERHRIRSEEDKDFSVMNLAELMAAA
                     AESTKTLSFLLAAVASISLLVGGIGIMNIMLVSVTERTREIGIRMAVGARTPDILLQF
                     LSEAVILSVLGGVTGIALGALVSLLISHFAGWPTVISPLSIALAFGFSALVGIFFGYY
                     PASKASALDPIEALRYE"
     gene            complement(4976..5680)
                     /locus_tag="PLY83_04525"
     CDS             complement(4976..5680)
                     /locus_tag="PLY83_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV29331.1"
                     /translation="MALIELAGIRKTYSLGEISVEALRGISLSIEKGEFVAIMGPSGS
                     GKSTLMNILGCLDTPTEGTYTLDGSDVSRLGKDELATIRNRHIGFVFQGFNLLPRTSA
                     LENVELPMLYKGLPKAERRRRAAEALERVGLAGREHHQPHQLSGGQQQRVAIARALVN
                     ESPLILADEPTGNLDTKTSVEVMEFLTELNRVAGITIVLVTHEPDIARFSRRLIMVRD
                     GLVVSDRHPDEGGMHA"
     gene            complement(5683..6978)
                     /locus_tag="PLY83_04530"
     CDS             complement(5683..6978)
                     /locus_tag="PLY83_04530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOV29332.1"
                     /translation="MTSLTKTKKRILAGVVLIAALAGGAWAWKVKFAEKPVQYTTEEV
                     SRGDITRVVSATGTIQAVNTVTVGSQVSGTIRRILVDYNSPVKKGQLLAEIDPALFLA
                     QARQAEAEVAQSRASLAEAEASLREAEREYERQKKLFASDFVAKSALESAETAVETAR
                     SRVESARAGVRSSAATLNQRLTNLGYTKILSPVNGVVTGKKVSEGQTVAASLSAPELF
                     TIAEDLSKMQVEAAVDEADIGYVQEGMNVTFTVDTYPEDTFSGKVRQVRLSPSTTENV
                     VTYTVIISAANPDLRLKPGMTANVSIETASVKDVLRVPNAALRFRPTATRAKTETASQ
                     VAPPPPPDGGSAGGEGVSKKASGLTGKTAALQPSVWVLETKKGSAPSLRRVPVTVGLT
                     DGSWTEVSGDLSEGEAVVTGTLGNGSRGSSGGNLPRRMF"
     gene            complement(6975..8255)
                     /locus_tag="PLY83_04535"
     CDS             complement(6975..8255)
                     /locus_tag="PLY83_04535"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOV29333.1"
                     /translation="MKKIPWVILLLLISSFAAWANDSPAPSAPALTLEQCLKMAFLHH
                     PEIVSSAWKVRRYESQAQLAKSSARPTVALGATLSDSENAEARSASDLTVKQLLTDGG
                     KTRATIAGAAYDLSAARKDAERTWQERAFAVKEAFFNLLRTREDASVAIETVALYESQ
                     LKQAQAFYRAGSSSKIDVTTAEVNLSQARLELAKAESAAASALAVLENEIGTALPDRK
                     MPLEVPEERNGSIPSLETALGEALSARVDVQAAEDRVLSAKEALAAASAGMNPSLFLS
                     GGWGFSDSSSGWEDEWSAGLSLSVPLYDGGATAARTEAARADLKIVQSDREKLINSVR
                     LEVETALLDLETASQQVKTAEAALRQARENLDLARGRYRVGVGTSLEVTDAAEKYSKA
                     AKSLVQARYDRQVAAAALEKALGRTAGIPKEETK"
BASE COUNT         1432 a         2821 c         2588 g         1552 t
ORIGIN      
        1 cttttttcgt tccgcggcat ggcggaacga ctggaagccc tcctgcaaat ccccctggaa
       61 cggccgtcct ggttccttca gacgggggcg atcggagccg cggcctgcgc ctggagccag
      121 gacaaccgga cccgcctgga tttcgagcag gtggaggcgg ctctggcctc tttctcgagc
      181 ggccaggttt ttgcgcctcc cctctctgat tcccttcgcc gggtctttct cgtccgccga
      241 agcccggccg aacaggaaaa caagcctgtc agccgacggc tcgtggacgc cgcgctgggg
      301 gtggacggag ggtccaccac gacaaaagcg gccctggtcg atgccgaaag ccataccgtt
      361 ttagacagcc tgtacgtccc cacccatggc gacccggaag gggccctgaa aaccgtcctg
      421 aggcacctgg cccgaaacgc ggaccaggtg cgaattctcg cggtctgcac cacgggatcg
      481 gcgagaaaac tgtacgaacg catcctcacc agcccctcca ggcgcgccgc actggaagcg
      541 gaggggttct tcgtccccga cggcacggtg gacgagatca cctgccatgc aaggggcgtc
      601 aaggaattcg accccgaggt cgacacgatc ttcgaaatcg gcgggcagga catgaagttc
      661 accaccttca agaagaggga agggcgcgcc acgacggaag tccaggaggc ccggatgaac
      721 tattcctgcc aggccggtgc ggggcagacc ctggaaaaca tggccgcgct gctggggctg
      781 gacgtgaaaa ccactctcca ggaggccgcc ctcgccgccc ggcgcgttcc cgtcatcgac
      841 gcgacttgcg gggtcttcat ggaaatggac gaaaaacgac tggtcgccga gggcttcccg
      901 gtggaagaga tcgccgccgc gatcgtccgg gcgaccgtcg ccgggtactt ccacaaattt
      961 gtcgggggat cgcgcacggt aggctcccac tgttcctgcc agggaggccc gtcgctcggc
     1021 agggcggtcc tggccgccct gagccaggtt gctgaccgtg agatcttcgc ttaccccggg
     1081 cgggagcttt tcggggcaat cggggcggct ctctgcgcac tcgatgccgt ttcggaggcc
     1141 acggccgcgg gacgcaaaac cgcaagcgcc ttccggggat ggcaggtcac ggagagcccc
     1201 ctcgagagcg ggaccgttct ctgctcttcc ctccacggcg cggattcctg cggcctcagg
     1261 gactgcaagc tcaggacgtt ccggatcgaa ggggaaacga tcgtgaccgg agggttttgc
     1321 ccgcggggca actcggaggc caccggcacc ctcaagccgg actacgtgga ggtttaccac
     1381 cgcctcctgg aacggcattt cacgggcacg gcagccgttc ggggaggagc cgtccgtgcg
     1441 ttcgaccgac cgaccgtcgg aatcaagcgg agcggcgaaa cccttgggga gtgggggatc
     1501 tggtgcgcgg cgctcctcga ttccctcggg tttcatcccg tcctctcgcc cgtctcggat
     1561 gaagggatcg tggaccgggg catccagctc gcccgaaccg acctttgctt tgccatgaaa
     1621 ctttccctgg gacactccga ttacctggcc aacaccgaac aggtcgattt cctctttcac
     1681 ccggcgttta tccatcgatc ggagggagag gggatccccc ggctgaaata ctgcgtctac
     1741 accgaggggg agggcttcct tgccacggaa acattggggc tcccgccgga acgggtcctt
     1801 gcccccgtct ggaaattcgg ggatccttac gggatggccg aatccctcca ggccgaactg
     1861 ggacgaatcg gacgcaaaac cccgatgggc aggatcctgg aagcctttga aaaggcggac
     1921 gccgcccggg aggcatttct cgaagccctc gccgccgtcg gggagacctt cctgaaacgg
     1981 gtcgccgaca gcggtcagcc gggctacgtc ggcctggggc gcgagtacgt gatctgcgac
     2041 ccaatggcct cttcaggaac cgggagcctt ttctcccgca tccggggaat gaactacatc
     2101 cccatggtct tcctctggaa ggcctaccga aacgtgccgg tccacgagct ggtggagaac
     2161 gaatactggt tcaacaacgc ccagatcctg aaagcctccg ttttcgtcgc caggacgagc
     2221 ggactgttcc cgatccggca gatgaacttt gcctgcggcc ccgattccat caagttcttc
     2281 atggagcgcg aaattttcga gcgggccgat aaaccctttc tgcacctggt caccgacgcc
     2341 cagaccaaca gcgccccgtt tgccacccga gccgaggccc acgaacggat cgtcgcccag
     2401 tcccgaccga ggccgatcca gacctgggtg gaactgctgc cccctctccc gaaagccccg
     2461 aaagggaatg cccgcacgtg gatcctcccc tccatgggac acgtcaaccg cctcggaggg
     2521 gctcttttgc ggcactgggg catccaggcc gccgcaatcc cgactgccgg gcaatccgcc
     2581 cgggaatggg cccgcagaac cattcccgtt gaaacctgct atccgctcaa gggagtgacg
     2641 ggagacgtcc tggccttcct ggaagcggaa tcggcccgaa tcggccgcca ggccgtcctg
     2701 gaccggtacg ccgttttcct gccgcgggcc agcggcccct gccggctggg gaaatacgac
     2761 gaggtcctcc gccattgtct cgcgcaggcc ggtttcgggg acctgcccgt cctgggtccg
     2821 cacatcggca agaactaccg ggaccttccc ttccttcccg accgtgcccg aggcaacccg
     2881 gcggccctgt tccggtttgt ccgcgaggcc cttcacgcgg tgctcttttc cgatttcctg
     2941 gatgacctct gcctgaggct gcgcccctac gcccccgaca ccggacggtt cgaggaaggg
     3001 gtggaatccg tcctggaaac aacaaccgca gtgatcgagc aggaaggcct ttcggaaacg
     3061 gtcttgcgga aacggatccg cgaaagcaaa aaaaccctgg cttccctgag ccgtccggat
     3121 catccccggt ttcccttcgt cctcttcatc ggggagatct acatgcggct tcatgaccct
     3181 tacacggatc acgtgatccg gctcctcgag tccgagggcc tcgagatcat ccgaagcgga
     3241 atcggggaat ggctggacta cacgaggtca gcgacgcgcc gcgacgggcc gaacctggcc
     3301 aatctcctgg ccgcagcggc cctttctttt tccaggagcc gcttcgtcca gctcggaggg
     3361 gttcccttcc cggaaagggc ccgcctgccc gaaccggctg aagccatcga agccctcgaa
     3421 cgggaacgga ttttctccgg cgagatcggc ggcgaatcgc ccattaacat cggcattttc
     3481 aacgagttca tgaaagacag gcttccttcg attgccgggc ttccggtttg cgggattttc
     3541 cattgcggcc ccttcacctg catgcacgag accctggcgg cttccctcat gaccgccctc
     3601 gcgaaaaact ggagggcatc ccacccggat gccctcctgc cgatccttca cgccagtttc
     3661 ggcgactctc ccaatcccaa cctggaagcc gagatcgccg cctttcgcga ccaatgccat
     3721 gcgagagccc gcctggaatc cgctgcgcct cactcgtagc gcaaggcctc gatgggatcg
     3781 agggcggaag ccttggacgc ggggtaatag ccgaagaaga tcccgaccag ggccgagaag
     3841 ccaaaggcaa gcgcgattga aagcggcgaa atcaccgtgg gccagcccgc gaagtgcgat
     3901 atcagaagcg agaccagggc cccgagggca attcccgtca ctcccccgag gaccgagagg
     3961 atcaccgcct ccgacaaaaa ctgcagcagg atgtccggcg tccgggcccc gaccgccatc
     4021 cggatcccga tctcccgcgt cctctccgtg acggagacga gcatgatgtt catgatgccg
     4081 atccctccca cgaggagcga gatggaagcc accgcagcga ggaggaacga cagggtcttc
     4141 gtggactccg cggcggccgc catgagctcg gccaggttca tgacggaaaa gtccttgtcc
     4201 tcctcggaac ggatccggtg ccgctcggtg aggagcgcgt cgatctgctc gatggccgcg
     4261 gagatggagt tgctgtccgc cgcctggacg aggatgctcg agaccttccc ctggaaacgc
     4321 gaaccgaaca cccgcttctg ggccgtcgtg atagggatga tcagggtgtc gtcctggtcc
     4381 tgccccatgg cgtttgtgcc ctttttcccc agcacgccaa ccaccaccat ggggatcttt
     4441 ttcacccgga cgaccttccc gatgggatcc tcgcccccga acaggttgtc cacgaccgtc
     4501 tggccgagaa ggcagacctt cgccgcgctt ctcacttccg aagtgttgaa gctgcggcca
     4561 gaaccgatgg tccagttgcg gatgtcaagc atcgggggag tggtcccttc cgcccgggtg
     4621 gaccagttgc tgttcccgta gacgatctga accgatccgg ccgcggtggg ggcggctttc
     4681 cccacggcgg ggcattcctt cacgatcgcg tccgcgtccg caaaggtcag cgtcggggag
     4741 ccgccccagc cggtccgggc ccccccgctc gtcgtgctgc ccgaacggac aaccaggagg
     4801 ttggttccca tacttgccag ctgggcctcc acgttccggc tggctcccgt gccgatggcg
     4861 aacatggcca cgaccgccgc cacgccgatg acgatgccca gcgtcgtcag ggcggagcgc
     4921 atcttgttcg cccgcagcga gcgaagggaa acgtggagga tttctccgaa ggcgatcatg
     4981 cgtgcattcc cccctcgtcg ggatggcggt cgctcaccac gaggccgtcc cggaccatga
     5041 tgaggcggcg cgaaaagcgg gcgatgtccg gctcgtgggt caccaggacg atcgtgatcc
     5101 ccgcgacgcg gttcagttcg gtcaggaact ccatgacttc gacgctcgtt ttcgtgtcga
     5161 ggttgcccgt cggttcgtcc gccaggatca ggggggactc gttcaccagg gccctggcaa
     5221 tggccacccg ctgctgctgt cccccggaaa gctggtgagg ctggtggtgt tcgcgccctg
     5281 ccaggccgac cctttcaagg gcctccgccg cgcggcgcct cctttccgct ttcggcagcc
     5341 ccttgtagag catcgggagc tcgacgttct ccagggccga ggtgcgggga aggaggttga
     5401 aaccctggaa aacgaacccg atgtgccggt tgcggatcgt ggccagctcg tccttcccga
     5461 ggcggctcac gtcggagccg tccagcgtgt aagtcccttc cgtcggggtg tcgagacagc
     5521 ccaggatgtt catcagggtg gacttgcccg agcccgaggg ccccatgatc gccacgaact
     5581 cgcccttctc gatggagagg gagatccccc gaagggcttc caccgagatc tccccgaggg
     5641 agtaggtctt gcggattccg gccagttcga tgagcgccat ggctaaaaca tccttcgcgg
     5701 gaggttcccg ccggaggaac ccctcgagcc gttcccaagc gttcccgtca cgaccgcttc
     5761 gccttccgaa aggtctcccg aaacctccgt ccagctgccg tccgtcaggc cgaccgtcac
     5821 cggcacccgg cggagcgagg gagccgatcc cttcttcgtt tcgagaaccc agacggaagg
     5881 ctgcaaagcc gccgttttcc cggtcaggcc gctcgctttt ttcgagaccc cttctccccc
     5941 cgcacttccg ccgtccgggg gcggaggagg ggcaacctgg gaagccgttt ccgtcttcgc
     6001 ccgggtcgcc gtcggccgaa accgcagggc cgcgttgggg actcgcagga catccttcac
     6061 cgacgcggtc tcgatgctga cgttcgcggt catccctggt ttcagcctca ggtcggggtt
     6121 tgccgccgag atgatgacgg tgtacgtcac gacgttttcc gtggtcgacg ggctgagtcg
     6181 aacctgccgg accttccccg agaacgtgtc ctcgggatag gtgtccaccg tgaaggtgac
     6241 gttcatcccc tcctggacat agccgatgtc ggcctcgtca acggcagcct ccacctgcat
     6301 cttggaaagg tcctcggcga tcgtgaacag ttccggggcc gacaggctcg ccgccacggt
     6361 ctgtccctcg ctcacctttt tccccgtcac gaccccgttc acgggggaaa ggattttcgt
     6421 gtaccccagg ttggtgagcc tctggttcag cgtcgcggca gaagagcgga caccggcccg
     6481 ggccgactca accctcgatc gggctgtctc caccgcggtt tcggccgatt cgagagcgct
     6541 tttcgccacg aaatcggagg caaacagctt tttctgcctc tcgtactcgc gctcggcctc
     6601 ccggaggctg gcctccgctt ccgccaggct ggccctggac tgtgccactt ccgcttccgc
     6661 ctggcgggcc tgcgccagga agagggccgg atcgatctcc gcgaggagct ggcccttttt
     6721 cacgggcgag ttgtaatcca ccaggatcct ccggatggtt ccagaaacct ggctgccaac
     6781 cgtcacggtg ttcacggcct ggatcgttcc ggtggccgaa acgacccggg tgatatcccc
     6841 cctggaaact tcctccgtcg tgtactggac cggtttttcc gcgaacttca ccttccaggc
     6901 ccacgctcct cccgccaggg ccgcgatgag gacaaccccc gccaggatcc tcttcttcgt
     6961 tttcgtcagg cttgtcattt cgtttcctcc ttcgggattc ccgccgtcct gccgagagcc
     7021 ttttccaggg ccgccgctgc gacctgccgg tcgtaacggg cctggaccag ggattttgca
     7081 gccttgctgt atttttccgc cgcgtcggtc acttccaggc tcgtgccgac cccgacgcgg
     7141 taccgccccc gggccaggtc gaggttctcg cgggcctgtc gcagggcggc ctcggcggtc
     7201 ttcacctgct gcgaggcggt ttcgaggtca agcagggccg tctccacttc aagccggacc
     7261 gagttgatca gtttttcccg gtcgctttgg acgatcttca ggtccgctct cgcggcctcg
     7321 gtccgggctg ccgtcgcccc gccgtcatac aggggaaccg agagagaaag cccggcgctc
     7381 cactcgtctt cccagccgga ggaagagtcg gaaaaccccc atcccccgct caggaatagg
     7441 gagggattca tccccgcgga agctgcggcc agtgcctcct ttgccgagag gacccggtct
     7501 tcggccgcct gaacatccac cctggccgac agggcttccc ccagcgccgt ctccagggac
     7561 gggatcgaac cgttgcgttc ttccggaacc tccaggggca tcttcctgtc cggcagggcc
     7621 gtcccaatct cgttttccag gaccgccagc gccgaggctg ccgccgattc tgccttggcg
     7681 agttcgagcc tcgcctggct gaggttgacc tcggcggtcg tcacgtcgat cttcgaggag
     7741 gaacccgccc tgtagaacgc ctgcgcctgc ttcagctggc tctcgtagag ggcaaccgtc
     7801 tcgatggcga ccgacgcgtc ctcgcgcgtt cggaggaggt tgaaaaacgc ctctttcacg
     7861 gcaaaggccc tttcctgcca ggtccgttcc gcatccttgc gggctgcgga aaggtcgtag
     7921 gctgccccgg cgatcgttgc acgggttttg ccgccgtccg tcagaagctg cttcacggtg
     7981 aggtccgaag cggaccgcgc ctcggcattt tccgagtccg aaagggtggc cccgagggca
     8041 accgtcggtc tcgccgagga cttggcgagc tgggcctggc tttcgtaacg gcgaaccttc
     8101 caggcggagg acacgatttc gggatgatgc aggaacgcca tcttcaggca ttgctcgaga
     8161 gtcagggcag gggcgcttgg ggcaggcgaa tcattggccc aggcggcaaa ggatgaaatc
     8221 agcagcaaca ggatcaccca agggattttt ttcacttgcg atcaacctcc tcgaaacgcg
     8281 gatgcctttc ggccggcctt ctccattgta gcccggggat tttaagcggg tgttccgtcc
     8341 tgttgtcttt ccgtaaagaa tcgtaaaggc tcctttacac gagtcaacct ttc
//
LOCUS       DAOOVN010000038         7776 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_5678_length_7776_cov_14.515218, whole genome shotgun sequence.
ACCESSION   DAOOVN010000038 DAOOVN010000000
VERSION     DAOOVN010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7776
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5678_length_7776_cov_14.515218"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="PLY83_04540"
     CDS             complement(<1..843)
                     /locus_tag="PLY83_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOV29334.1"
                     /translation="MKIIDCHVHVYPPHIITNWEKIAEKEPYFARLARGKVHKWATAE
                     DALNAMDEAGISESWIFGFAFRDLGLCRECNDYVIESQARFPGRFKGFAVVPPLARGT
                     EEEIRRCRKKGLWGVGELFPEGQGFDLSDIRQTWRLAGTCHELGIPVIFHTAEPVGHD
                     YPGKGNVGPREAAMLCSNHPELRVVFAHMGGGLWLYELMPEMARVLENAWYDTAALPF
                     LYDAAVYRSAWASGLGRKILFGSDFPILKWARHSRQIEESGLTQGQKESLLAGNADRF
                     FGTLP"
     gene            complement(840..>1211)
                     /locus_tag="PLY83_04545"
     CDS             complement(840..>1211)
                     /locus_tag="PLY83_04545"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOV29335.1"
                     /translation="QLFFDNELFYRFRDEARRRGITVPIVAGIFPVLNAAQVQKIVSL
                     CGVSLPPRFTRIIARYANSPAALAEAGVAYATDQIVDLLAAGVDGIHLYTMNRPETTR
                     RIMGNIDQIRKTASPEGREKG"
     assembly_gap    1212..1311
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1312..1619)
                     /locus_tag="PLY83_04550"
     CDS             complement(<1312..1619)
                     /locus_tag="PLY83_04550"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOV29336.1"
                     /translation="MFIRDLFGKNGPVVSFEVFPPKADAPVEGVYRTIEALKDLSPGF
                     ISVTYGAGGSSRDRTVEIAARIRNGYGIEALAHLTCRSHTAEEIDDICEELLDEGVQN
                     "
     gene            complement(1623..4034)
                     /locus_tag="PLY83_04555"
     CDS             complement(1623..4034)
                     /locus_tag="PLY83_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOV29337.1"
                     /translation="MLDLVRWLREKNSIVVLDGGMGTMLGDAGWRPPLLPEEMNLVNP
                     DAVREIHEAYVEAGALLIETNTFGGSAVKLAHRGLDGETREINRQAVSLARQAAGGRA
                     LVAGSIGPSGRLVEPLGNLAFEEAVSSFLPQVQGLLDGGVDFFLVETMIDLREAKAAV
                     MAIKDAGRGLPFVVSFTFDRNGCTMTGTPPEVAMAWATAVGASGVGVNCGTGPEDTVE
                     TVRRLRRAGALPVFAYPNGGLPSQGDWLAPDPFAEACAGLAEAGATVIGGCCGTTPRH
                     IQALSRRLAGIRPVHGSPRRQTALASRTRLSLCGEGEPLLVVGERINPSRKGPLREAM
                     IAGNWAQVREEARLQAEAGAEVIDINGGIPGQDRLGLISAAVTAVEGAVNLPVSIDSE
                     DPLILERIARSVAGIPLLNSVTCEPEVLERGLSAAVKTGSALVVLTMDSKGIPEEAKG
                     RLALAERAAEEGEKIGLPRTMLFVDPLTLAAGAGDGNARLTLESLRGLSSIGTRSILG
                     ISNISFGLPKRSLINRTFLAMAMACGLDAVIANPLDEALMSCAAAGNVLAGRDPGAKR
                     YIAAFSGKEPSETTSPQLHAAPSFGLDLKEAILRGEKEAAISAGRTFLGEGGHPMELI
                     QDRVLPALEEVGRLYECGDFFLPQLLASSEAAQAVCRLAEESLLAGGKTLETRGTVLL
                     ATVEGDLHDLGKNVVAMVLKSRGYKVVDAGKDVPAERILEAAVREKADLVGLSALMTT
                     TVERMGEVISLGRARGMTCRFVLGGAAVSADFAEKIGADGYARDAIEAARLVDRLLAQ
                     EKGGE"
     gene            complement(4196..4573)
                     /gene="rplL"
                     /locus_tag="PLY83_04560"
     CDS             complement(4196..4573)
                     /gene="rplL"
                     /locus_tag="PLY83_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOV29338.1"
                     /translation="MTREEIIKAIEEMSVLELSQLVKDLEEKFGVSAAAPAMMAMPVA
                     AAAAAAPVEEEKTEFDVILKSAGSNKIGTIKVVREITSLGLKEAKELVDNPPKPVKEG
                     VSKEEAEEIKKKLVEAGAEVEIK"
     gene            complement(4638..5168)
                     /gene="rplJ"
                     /locus_tag="PLY83_04565"
     CDS             complement(4638..5168)
                     /gene="rplJ"
                     /locus_tag="PLY83_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOV29339.1"
                     /translation="MPTEYRVQQVAMLKEMLGKAETIFVCEYRGLTVAKSTEIRSRIR
                     KAGGEMKIARNTLMRRALEEMGHPAPEELTSGPNVYTLVYGEISEVAKALRDFSKEKG
                     NEALIVKGGIMGKEILSQAQIFALADLPSREQLVAQVVGTIAAPLRGLLTVLSGPARG
                     LVTCLSQVQEQKEKAA"
     gene            complement(5397..6110)
                     /gene="rplA"
                     /locus_tag="PLY83_04570"
     CDS             complement(5397..6110)
                     /gene="rplA"
                     /locus_tag="PLY83_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOV29340.1"
                     /translation="MAKKSKRYQAIKANVDREKLYGLREALDLCKTNATAKFNESVEV
                     HIRLGVDPRQADQQVRGTVPLPHGTGVTKRVLVITTGEKLKEAEEAGADFVGSDDMVQ
                     KIESGWLDFDAVITSPDMMKSVGRLGKILGPRGLMPSAKTGTVTFDVADAVKEVKAGR
                     VEFRLDKFGIIHKAIGKTSFTTDQLYDNARAFFQAILKAKPSAAKGTYVKSFSVASTM
                     GVGIKVDPNAAQKEIAAVE"
     gene            complement(6168..6593)
                     /gene="rplK"
                     /locus_tag="PLY83_04575"
     CDS             complement(6168..6593)
                     /gene="rplK"
                     /locus_tag="PLY83_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOV29341.1"
                     /translation="MAKKVVGAVKLQLPAGKATPAPPVGPALGQHGVNIMEFCKQFNA
                     KTADQPGMIIPVVITVYADRSFSFILKTPPASVLLKKAAKIDKGSSVPNKTKVAKVTK
                     NQIREIAQLKMEDLNANDIEGAMQMIMGTARSMGIEVTD"
     gene            complement(6626..7171)
                     /gene="nusG"
                     /locus_tag="PLY83_04580"
     CDS             complement(6626..7171)
                     /gene="nusG"
                     /locus_tag="PLY83_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOV29342.1"
                     /translation="MMNEKNDRRWYIVQTYSGYENRVLANLEQRIASMGMEDRIFRVL
                     VPVEERVYVKDGKTKRVHRKVFPSYVLVEMILDDQSWYVVRHTPGVTGFVGAGNHPIP
                     LTEKEIQDIIGKIGKDQIKPKVEIDLKPGDVVRVKSGPFQGQVGPVAEVLPEKGKVKF
                     SVTVFGRETLVEADYQELERL"
     gene            complement(7247..7429)
                     /gene="secE"
                     /locus_tag="PLY83_04585"
     CDS             complement(7247..7429)
                     /gene="secE"
                     /locus_tag="PLY83_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOV29343.1"
                     /translation="MGKFLDFVREARAELRKVTWPGRQQVWYSTLVVIALTLLTSAVL
                     GVMDVLLTFVFKAVIR"
     gene            complement(7460..7536)
                     /locus_tag="PLY83_04590"
     tRNA            complement(7460..7536)
                     /locus_tag="PLY83_04590"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7500..7502),aa:Trp,seq:cca)
     gene            complement(7558..7707)
                     /gene="rpmG"
                     /locus_tag="PLY83_04595"
     CDS             complement(7558..7707)
                     /gene="rpmG"
                     /locus_tag="PLY83_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOV29344.1"
                     /translation="MADIIGLTCTECKRRNYVTLVNKKKMGKKLEKKKYCKWCDKHTL
                     HKESK"
BASE COUNT         1434 a         2469 c         2098 g         1675 t
ORIGIN      
        1 gggcagggtg ccgaaaaatc ggtccgcgtt tccggcaaga agcgattctt tttgtccctg
       61 ggtcaagccg gactcttcga tctgccggga atgacgcgcc catttcagaa tcgggaaatc
      121 gctcccaaaa aggattttcc ttccaagccc cgaggcccag gcagaccggt agaccgcagc
      181 gtcatagagg aacgggagcg cggccgtgtc gtaccaggca ttttccagga ccctggccat
      241 ttccggcatg agttcgtaga gccagaggcc gccccccatg tgagcgaaaa cgacacgcag
      301 ttcaggatga ttggagcaaa gcatggccgc ctccctgggg ccgacgtttc ctttcccggg
      361 ataatcatgc ccgaccggct cggcggtatg gaagatcacg ggaattccca gctcatgaca
      421 agttccggca agacgccagg tctgccggat gtccgagagg tcaaagccct gcccctcggg
      481 aaagagttcc cccactcccc acagaccttt tttcctgcaa cgccggattt cctcctctgt
      541 ccccctggcg aggggaggaa ccaccgcaaa ccccttgaat cgccccggga agcgagcctg
      601 gctttcaatg acataatcgt tgcactccct gcagagcccc agatcccgaa aggcgaaccc
      661 gaaaatccag ctttcgctga tgcccgcttc atccatcgcg ttcagggcgt cttccgcggt
      721 cgcccacttg tggacctttc cccgggcaag cctggcgaaa tagggttcct tttcggcaat
      781 cttttcccag ttcgtaatga tgtggggggg gtaaacatgc acgtggcaat cgattatttt
      841 caaccctttt cacgtccttc cggcgatgcc gtctttctga tttggtcgat atttcccatg
      901 atacgcctgg tggtctccgg gcggttcatc gtgtagagat gaattccgtc cacgccggcg
      961 gcgaggaggt cgacaatttg atcggtcgca taggcaactc cggcttcggc tagagccgca
     1021 ggggaattcg catagcgtgc gatgatgcgg gtgaaccgcg gcggcagcga aacgccgcag
     1081 agagaaacga tcttttggac ctgggctgcg ttcaggaccg ggaagatgcc cgctacaatg
     1141 ggaacggtga tgcccctcct gcgggcctcg tcacgaaacc ggtagaacag ttcgttgtca
     1201 aaaaaaagct gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn natattctga
     1321 accccctcgt ccaggagttc ttcgcagatg tcatcaattt cctcggcagt atgggatcga
     1381 caggtaaggt gcgcgagagc ctcgattccg tagccgtttc ggatcctcgc tgcgatctcg
     1441 accgttcgat cccggctgct tcctcccgct ccgtaggtga cgctgatgaa gccgggggac
     1501 aggtctttca gggcctcgat cgtcctatag accccttcga ccggggcatc ggcttttgga
     1561 gggaaaacct cgaaagaaac gacagggccg tttttcccga aaaggtcgcg gatgaacacg
     1621 gtttattccc ctcctttttc ctgtgcgagc aggcgatcga caagacgcgc ggcctcgatg
     1681 gcatcccggg cgtacccgtc agccccgatt ttttcggcaa agtctgcact cacggcagct
     1741 ccgccgagga caaagcggca ggtcatccca cgggcccgtc ccagggagat gacttcgccc
     1801 attcgttcaa ccgtcgtggt catcagggcg gaaaggccca ccagatccgc cttctcgcga
     1861 accgccgctt ccaggatccg ctccgcgggg acgtccttgc cggcatccac gaccttgtac
     1921 ccacggcttt tcaggaccat cgccacgaca ttctttccca ggtcatggag atccccttcc
     1981 acggtcgcca ggagaactgt cccgcgcgtt tccagagtct ttccacccgc cagcaggctt
     2041 tcctccgcca gcctgcagac ggcctgggct gcctcggagg aagcgagaag ctgcgggagg
     2101 aagaaatctc cgcattcgta caaccttccg acctcttcca gcgcggggag aaccctgtct
     2161 tggatcaact ccatggggtg accgccctcc cccaggaaag tccgaccggc ggaaattgcg
     2221 gcttccttct cccctctcag gatcgcttcc ttgagatcca gcccgaatga aggggcagcg
     2281 tgcagctgcg gggaagtcgt ttcggaaggc tccttccctg aaaacgcggc gatgtagcgt
     2341 ttcgcaccag gatcgcgacc cgccaggacg ttcccggcag ccgcacagct catcagagcc
     2401 tcatccagcg ggttggcgat cacggcatcg agaccacagg ccatcgccat ggccaggaac
     2461 gtccggttga tcaggctcct cttgggaagg ccgaaggaaa tattgctgat cccaaggatc
     2521 gaacgcgtcc cgatcgatga aagtccgcgc aggctttcca gggtcaatcg tgcgttccca
     2581 tccccggctc cggcggcaag ggtcagggga tcgacgaaaa gcattgtcct aggaaggccg
     2641 atcttttctc cctcctccgc cgcccgttcg gcaagggcca gccttccctt cgcctcttca
     2701 ggaatccctt tgctgtccat cgtcaggaca acgagcgcag acccggtttt gaccgcggct
     2761 gaaagtcccc tttccaggac ttctggctcg cacgtcacgg aattcagaag cggaatcccg
     2821 gcgacagacc gagcgatcct ttccaggatc aggggatctt cgctatcgat cgaaaccggc
     2881 aggttaacgg cgccttccac cgcggtgacc gccgctgaaa tcaatcccag ccggtcctgg
     2941 cccggtatcc ccccgttgat gtcgatcact tcggctcccg cttctgcctg gagtcgagcc
     3001 tcttctctca cctgggccca gttcccggca atcatcgctt ccctgagagg gcccttccgg
     3061 gatggattga tcctctcccc cacgacgagc aggggttccc cttccccgca aagagatagg
     3121 cgggtccggc ttgcaagcgc cgtctggcgt ctgggcgatc cgtgaaccgg acggattcct
     3181 gccagccgcc gggaaagggc ttggatatgc cggggagtgg tgccgcaaca accgccgatg
     3241 acggtcgccc cggcttccgc aagccccgca caggcttccg caaaggggtc gggagccagc
     3301 caatcccctt gtgaaggcag ccccccgttc gggtaggcaa agacgggcaa tgctcctgcc
     3361 cggcgcaatc gacggaccgt ttcaacggta tcctcggggc ccgtgccgca gttcactcca
     3421 acccctgatg ctcccacggc cgtcgcccag gccatggcca cctccggcgg agttccggtc
     3481 atggtgcatc cgttgcggtc aaaggtaaaa ctgacgacaa agggaagccc cctccccgca
     3541 tccttgatcg ccatgaccgc tgccttcgct tcacgcaaat cgatcatcgt ctcgaccagg
     3601 aaaaaatcca cgcctccatc gagaagtccc tggacttgcg gcaggaagga actcacggct
     3661 tcctcgaaag ccaggttccc cagcggctcc accaggcgcc cggaagggcc gatggaaccg
     3721 gccaccaggg ccctgccgcc ggcggcctgg cgagcgaggg acaccgcttg gcggttgatc
     3781 tccctggttt ccccgtcgag ccccctgtga gcgagcttga ccgccgagcc accgaacgtg
     3841 ttggtttcga tcaggagcgc cccggcttcc acgtaggcct cgtggatttc cctgacggca
     3901 tccgggttga caaggttcat ttcttccggc agaagcgggg ggcgccagcc ggcatcgccc
     3961 agcatcgtgc ccataccccc atccagcacg acgatcgagt tcttttcccg cagccaccga
     4021 accaggtcca gcatcgccct tccctcccgt tttcgttccc ggcaattcaa atccggatgg
     4081 ttcgttttaa gaatgcatca taacggcagt tcggttccag ggcaagtaaa aaaaacgggg
     4141 gcggtccagt ggaccgcccc cgtttgatcg aatcctggtc cgaatcggaa gcaaactact
     4201 tgatttcgac ttcggctccc gcttcgacga gcttcttctt gatttcttcg gcttcttcct
     4261 tggaaacgcc ttccttgacg ggcttcggcg ggttgtcgac cagttccttg gcttccttga
     4321 ggcccaggct ggtgatctcg cggaccacct tgatcgtccc gatcttgttg gatccggcac
     4381 tcttgagaat tacgtcgaac tcggtcttct cctcttcgac cggggctgcg gcagccgcgg
     4441 ccgcaacggg cattgccatc atggcaggag cagcggcgga aaccccgaac ttctcctcca
     4501 ggtccttgac aagctgcgaa agctcaagaa cagacatctc ttcgattgcc ttgataattt
     4561 cctcgcgggt cattgatttc atcctccttg ttttttgtaa tcaggctgga acggggaatc
     4621 ccctgtccct caagcgatca ggctgctttt tctttctgtt cctgtacctg cgacaggcag
     4681 gtaaccaatc ctcgggcggg tccgctgagg acggtgagca gaccgcgaag cggagccgcg
     4741 atcgtgccaa cgacctgggc cacaagctgc tctctcgaag ggagatctgc gagggcaaaa
     4801 atctgcgcct ggctcaggat ttccttgccc atgatcccgc ctttcacgat cagggcttcg
     4861 ttccccttct ctttcgaaaa atcccgaagc gcttttgcca cttccgagat ttccccgtaa
     4921 acgagcgtgt aaacgttcgg tccggaggtc aactcctcgg gagccgggtg ccccatctct
     4981 tcaagagctc gccgcatgag cgtgttccgg gcgattttca tctcgccccc ggccttcctg
     5041 atacgggaac ggatctccgt gcttttggcc acggtcagcc cgcggtactc gcagacgaaa
     5101 atggtttcgg ccttccccag catttccttc agcattgcga cttgctggac acggtattct
     5161 gtcggcaaaa cattcacctc cttcacccaa acatgaaaaa tccccggacc tgccgggtcc
     5221 ggggacgaag ggcagtttcc gcaaaagggg gaccttcttg cggaagcact ggactctatt
     5281 ccctgaacct cagcaggcgg agctctctcc atttcgtgcg cccgcgcggc gcacaccggc
     5341 tatctacggt tcgtctcttt cagttgtaaa ggttcgcctc gctgcctgcg aaggtcttat
     5401 tcgaccgcgg cgatctcctt ctgggcagcg ttcggatcga ccttgatgcc gacccccatc
     5461 gtggaagcga ccgagaaact cttcacgtag gttcccttcg cggcggaggg ctttgccttc
     5521 aggatcgcct ggaagaacgc ccgggcgttg tcgtaaagct gatcggtcgt gaagctggtc
     5581 ttcccgatcg ccttatggat gatcccgaac ttgtcgaggc ggaattccac gcgcccggcc
     5641 ttgacttcct tgactgcatc ggccacatcg aatgtgaccg tgcccgtctt cgcactcggc
     5701 atcaggccgc ggggaccaag gattttgcca aggcgtccga cggacttcat catgtcgggg
     5761 gaggtgatga cggcatcgaa atcgagccag ccgctctcga tcttctggac catgtcatcg
     5821 gacccgacga aatcggcacc cgcctcttcg gcctctttca gtttctctcc ggtcgtaatg
     5881 acaaggaccc tcttcgtcac acccgttccg tgaggaagcg gcacggtgcc gcgaacctgc
     5941 tggtccgcct ggcgtggatc cacacccagg cgaatgtgaa cttcgacgct ctcgttgaat
     6001 ttcgcggtgg cattcgtctt gcagagatcg agcgcctcgc gaagtccata aagcttttcc
     6061 cgatctacgt tggccttaat ggcctggtat cgcttgctct ttttcgccat ttcgctaacc
     6121 tccctgtggt cgtcgtgggt ccgttccaga ccctgccaca cgttgcgcta atcggtgacc
     6181 tcgatgccca tggacctcgc cgtgcccatg atcatctgca ttgcgccttc gatatcgttc
     6241 gcgttcagat cttccatctt gagctgggcg atctccctga tctggttctt cgtgaccttg
     6301 gcgaccttcg tcttgttcgg aaccgaggat cccttgtcga tcttcgcggc tttcttcaaa
     6361 agcacgctcg cgggcggggt cttcaagatg aaggagaagc tccggtccgc gtagaccgta
     6421 atcacgacag gaatgatcat accaggctga tcggccgttt tcgcgttgaa ctgcttgcag
     6481 aactccatga tgttgacgcc gtgctgcccc agcgcagggc ccacaggcgg tgccggggtg
     6541 gccttgcctg cgggaagctg tagcttcact gccccaacga ctttcttggc catgtttctt
     6601 ccaacctcct ttcccgagaa gttgcctaga gcctttcgag ctcctgataa tccgcttcga
     6661 ccagcgtttc gcggccgaag accgtcaccg agaacttaac cttgcccttc tccgggagca
     6721 cttccgccac cgggccgacc tgcccctgga agggaccgct cttgacccgg accacgtcac
     6781 cgggtttgag gtcgatttcc accttcggct tgatctggtc tttcccgatc tttccgataa
     6841 tgtcctggat ttccttctcg gtgaggggaa tcgggtgatt tccggctccg acgaaaccgg
     6901 tcaccccggg ggtgtgacgg acaacgtacc aagactgatc gtccaggatc atctcgacga
     6961 gcacgtaact gggaaaaact ttccggtgga cgcgcttcgt cttcccgtcc ttgacgtaga
     7021 cgcgctcctc gacggggacc aaaacacgaa agatgcgatc ctccatcccc atcgaggcga
     7081 ttctctgctc gaggttcgcg aggacgcgat tttcatatcc ggaatacgtt tggacgatgt
     7141 accagcgcct gtcgtttttt tcgttcatca tcaaaaaggg gggctcagct ggcccccgca
     7201 ccccctgcca gatgccctga agtcagcggc ttttcagggc ttacccctac ctgatgaccg
     7261 ccttgaagac gaaagtcagg aggacgtcca tcacaccgag caccgctgat gtcagaagcg
     7321 tcaaggcgat cacgacgaga gtcgagtacc acacctgctg ccttcccggc caggtcacct
     7381 tgcgcagctc ggctctggct tcacgaacga agtcgaggaa ttttcccaca gcctcggcct
     7441 cctgtctttt taaaaagaat ggcaggcccg gcaggattcg aacctgcaac ccccggattt
     7501 ggagtccggt gctctaccaa ttcgagctac ggacctgcga gcacgcgcta ttctacacta
     7561 cttggattcc ttgtgcaagg tgtgtttgtc acaccacttg cagtatttct tcttttccag
     7621 cttcttcccc atcttcttct tgttgacgag ggtcacgtaa ttcctccgct tgcattccgt
     7681 acaagtcaat ccgatgatgt cagccattcc cttttcggct cctagtcgag aatctcggtg
     7741 acgacgccgg cacccaccgt gcggccgcct tcccgg
//
LOCUS       DAOOVN010000039         7069 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_6332_length_7069_cov_9.949672, whole genome shotgun sequence.
ACCESSION   DAOOVN010000039 DAOOVN010000000
VERSION     DAOOVN010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7069
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6332_length_7069_cov_9.949672"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PLY83_04600"
     CDS             <1..320
                     /locus_tag="PLY83_04600"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOV29345.1"
                     /translation="LVLETVGKATWGRSIASLRRGGRIAFCGSTAGAEVHVNLQEIYR
                     KEITALGTYGGTPEEIRRVIRLAEQKLIRPVIDTVLPLRDAVLAHQRMEKAEHFGKIV
                     LAP"
     gene            complement(342..1148)
                     /locus_tag="PLY83_04605"
     CDS             complement(342..1148)
                     /locus_tag="PLY83_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOV29346.1"
                     /translation="MLEALQFPFMRNALLAGLWVSLACGIIGTLVVANRLVFLAGGIA
                     HSAYGGVGLAIFLGFSPFLGAMGFSLAMAVLLAVLTSRNKSRADTFVGVLWAVGMALG
                     VLFVDLSPGYRADLMNLLFGSILTVSSRELWMMAGITLLVAIAVGLKYRDLLALSYDP
                     EFAATRNVKVSSLYTLLLLLVAAGTVVAIQAVGLILVIALLTIPPILAERSCRSLASM
                     MGVSSAWSALFIFLGLDLSYRYNLTSGATIILVGAFFFFLHSIWTHRRQA"
     gene            complement(1141..1941)
                     /locus_tag="PLY83_04610"
     CDS             complement(1141..1941)
                     /locus_tag="PLY83_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOV29347.1"
                     /translation="MENREIDIELSGVRFAYNGSGPVLEGVSLSVPRGEFLAVIGPNG
                     GGKSTLLRLILGLLEPQEGTVRLLGRPPRESVQRVGYVPQETNLNREFPISVEDVVLM
                     GRFSGAAMRWIPSSEDRQVAEEVMERLGVLDLRRKRVGELSQGQRQRVAIARALAVRP
                     ALMLLDEPTASVDVQTQAALYQILRELNRTLTVVMVSHDLTAIASCATSVACVNRSVF
                     FHDRAELTEEMVQGAYGSCPVELIAHGIPHRVLHRHENGNGSERKGHA"
     gene            complement(1976..2827)
                     /locus_tag="PLY83_04615"
     CDS             complement(1976..2827)
                     /locus_tag="PLY83_04615"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOV29348.1"
                     /translation="MKRWSIGLFLLLLPLLAVGFLLHRPVSAGPMKVFVSIAPQKYFV
                     ERVGGNRVQVFVLIPRGADPHTFEPRPADMVELARSAAWFTIGVPFEGNLYPRISSAN
                     PALTLVRTEEGIERAGSGDEGVDPHIWLSPRLVKRQVERILAGLIQADPAGERVYRAN
                     ARRFQAELDALDREFKMLFAPVRGKSFWVYHPSWGYLASDYGLNQVAIEAEGKEPGGA
                     DLARFIETAREKKVRVIFVEPQSSARSARTIADSIGARLVPADPLAPDWADNLRKVAR
                     AIRQAAR"
     gene            complement(2824..3270)
                     /locus_tag="PLY83_04620"
     CDS             complement(2824..3270)
                     /locus_tag="PLY83_04620"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOV29349.1"
                     /translation="MENACQAAKILKSIGARVTPARVLMLETLHSGCGLFTAQALHED
                     LPEGTADLATVYRFLALLVEKNLAREVTGTDGVLYYEMACSHNPSHPHFECRSCGRIY
                     CLEGYLPEEWKHFPACPQGHGVESVSVVFRGTCSNCLGKLEKEAKE"
     gene            3385..3927
                     /locus_tag="PLY83_04625"
     CDS             3385..3927
                     /locus_tag="PLY83_04625"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOV29350.1"
                     /translation="MTPLELFRMFFKISAVTFGGGIAILAMAKTELRKRGFPEEQMAD
                     IAALATSLPGPIATSTAWLAGRKIAGFPGAFASVAGILLPPFFAILLLSGFVLAHRDS
                     ALLSAFFKGVLCAVAALVATLVYGEIRRTFTGKGRWLNLLPYALVIALILWAGLHPLA
                     AMACGVGLRLLLPEGTRWSC"
     gene            3915..4439
                     /locus_tag="PLY83_04630"
     CDS             3915..4439
                     /locus_tag="PLY83_04630"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOV29351.1"
                     /translation="MELLKLAWGFAQVGIGAFGGGISTLPLIRHILVDNYGWLSLDGF
                     DQVLALAQVTPGPIAVNAATFVGFQRAGLTGATVATLSVVVAPLLIVIAVAALLQCGS
                     KDCMDRFRNALRPTVAALLTLAFLPLARTAFSGIGTIALFAAGIGALNLRFFRENPPA
                     LFLLSGLAGLFLFL"
     gene            complement(4436..4801)
                     /locus_tag="PLY83_04635"
     CDS             complement(4436..4801)
                     /locus_tag="PLY83_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOV29352.1"
                     /translation="MIRKASELEKVRREAFTNGVGGGYATHALPPGEKMPGSRFQMVS
                     RIELDPGSSIGPHLHGENEEVYWILSGEGIYSEDGTEVPAGPGDVFIALQGHTHGLKN
                     TGSEPLEMIAVIVADRNPN"
     gene            complement(4844..5452)
                     /locus_tag="PLY83_04640"
     CDS             complement(4844..5452)
                     /locus_tag="PLY83_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOV29353.1"
                     /translation="MEKQASPDHPIDDLIARRWSPRAFSEKPVSREAVLSLLEAARWA
                     PSCFNEQPWSFIVATKEEPKAFETLLGCLREANRIWAGRASVLMVVSAKAAFDHNGKP
                     NRHAGHDVGLAVENLMLQAVAMGMFAHAMAGFDAAKVRETCGVPEGHDPLVAIAVGYP
                     GDPEDLPEDLKERELAPRERKPLESFVFGMSWGEAWNGLRNG"
     gene            complement(5731..6288)
                     /locus_tag="PLY83_04645"
     CDS             complement(5731..6288)
                     /locus_tag="PLY83_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOV29354.1"
                     /translation="MAGNGTDFYELKVAGLTRRLKKVRVAEHLVIASFVMLGDTKLIE
                     ACADALLPLLPKDTIEMLVCLEAKGIPLTHALAVRLGVDYVTVRKSVKSYMEDPLVVE
                     VQSITTAGKQLLVMDGVDRAKVEGRRVCVVDDVVSTGGSLRSVEKLLSAAGAKVVAKT
                     AVLLEEGGYDGADLVYLERLPVFPD"
     gene            complement(6301..>7069)
                     /locus_tag="PLY83_04650"
     CDS             complement(6301..>7069)
                     /locus_tag="PLY83_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOV29355.1"
                     /translation="IMLAFVAVNLYASEKFSGAVSFVSALAVWFITKDLAKTIIVSVL
                     ASTAVYNYLRYVAKKEIDAPEIHMENERFHFGNIEWCFWRNRAVFLGALGIACLNIGA
                     NISFGKITGSIANVNTNIDHLAIYSSLADILSTLFGGGPVEAIISGTATAPNPLRSSV
                     MMMGIMAAILLFRLLPVIGRYVHRSSIAGFLFILGTFVTFATNIQGAIASTPAFAGPY
                     GFSPWGMVIGATTLVSARWNPFYGLLAGLAIKLIFHL"
BASE COUNT         1304 a         2335 c         2140 g         1290 t
ORIGIN      
        1 atctcgtcct ggagacggtc gggaaagcaa cgtgggggcg cagcatcgca tccctgcgga
       61 gggggggacg catcgctttc tgcggatcca ccgccggagc ggaggtccac gtgaacctgc
      121 aggagattta ccgcaaggag atcacggccc tcggaacgta cgggggaact cccgaggaga
      181 tccgcagggt catccgcctt gcggagcaaa aactcatccg ccccgtcatc gacaccgtcc
      241 tgccgttacg ggacgcggtt cttgcccacc aacgcatgga gaaggcggag catttcggca
      301 agatcgtcct tgccccctga cccggggccg gaacgcgagc ttcaggcctg ccggcggtgc
      361 gtccagatcg agtggaggaa aaagaagaag gctccgacca ggatgatggt ggcgcccgaa
      421 gtgaggttgt accggtagga aaggtccagg cccaggaaaa tgaatagcgc gctccacgcg
      481 gaagagaccc ccatcatgga agcgagggaa cggcaggatc tctccgccag gatgggggga
      541 atggtaagga gggcgatcac cagaatgagc ccgacggcct ggatggcgac caccgtcccg
      601 gcggccacca gaagaaggag cagggtgtac aggctcgata ccttcacgtt tctcgtggcg
      661 gcaaactccg ggtcatagga aagggccaga aggtcccggt atttcagccc caccgcaatg
      721 gcgacgagaa gcgtgatccc cgccatcatc cagagttccc gggaactcac ggtaaggatg
      781 cttccgaaca ggagattcat gaggtcggcc ctgtagccgg gggaaaggtc cacgaaaagg
      841 acccccaggg ccatccccac cgcccagaga acgcccacga acgtgtcggc ccgggacttg
      901 ttcctcgacg tgaggaccgc caggagaacc gccatggcaa ggctgaatcc catggcaccg
      961 aggaacgggg agaaaccaag gaaaatcgcc agcccgacgc ccccgtaggc ggaatgggca
     1021 attccgccgg ctagaaagac caggcggttg gccacgacaa gggtcccgat gatcccgcag
     1081 gccaggctga cccacagtcc agccaggagt gcgtttctca tgaagggaaa ctgcagggct
     1141 tcaagcatgg cctttccttt cactcccgtt cccgttttcg tgccggtgca gaacccggtg
     1201 cgggatcccg tgggcgatca gttccaccgg gcagctgccg taggccccct ggaccatttc
     1261 ctcggtcagt tcggcccgat catggaaaaa gacgctgcgg ttgacgcagg ccaccgacgt
     1321 ggcgcagctg gcaatggccg tcaggtcatg gctcaccatg accaccgtga gcgtccggtt
     1381 caactcccgg aggatctggt agagggctgc ctgggtctgc acgtccacgc tggcggtggg
     1441 ctcgtcgaga agcatcaggg cggggcggac cgctagcgcg cgggcaatcg cgacccgttg
     1501 ccgctgcccc tgggacagct ctcccacccg ctttcgccga aggtcaagaa cccccagccg
     1561 ctccatgact tcttcggcca cttgccggtc ctcggaagaa gggatccacc gcatcgccgc
     1621 cccgctgaat cgccccatga ggacgacgtc ttccaccgaa atcgggaatt cccggttcag
     1681 gttcgtttcc tggggaacat acccaacccg ctggaccgat tcccggggag gacggcccaa
     1741 aaggcggacg gttccctcct ggggttccag gaggcccagg atcagccgaa gaagcgtgct
     1801 ttttcccccg ccgttcgggc cgatgaccgc aaggaactcg cccctaggga cgctcaagga
     1861 cactccctca agaacgggcc cggagccgtt gtaggcaaac cggacacccg aaagttcgat
     1921 atcgatctcc ctgttttcca acgggctttt cctccccacg ccctaacgcc ggaagctatc
     1981 gtgcagcctg ccggatggcc cgggcgactt ttctcaggtt gtcggcccag tcgggggcaa
     2041 gcgggtcggc aggaacaagc ctggcgccaa tggaatccgc gatggtccgg gcactccggg
     2101 cggaagactg gggttcgacg aagatcactc ggaccttctt ctcccgggcc gtctcgatga
     2161 agcgggccag gtccgcccct cccggctctt tcccttccgc ttcgatcgcc acctggttca
     2221 acccgtaatc ggaagccagg tagccccagg aagggtggta gacccaaaac gattttcccc
     2281 ggaccggggc aaagagcatc ttgaattccc ggtccagggc atccagctcg gcctggaagc
     2341 gccgggcatt ggcccggtag accctttccc cggcagggtc tgcctggatc agtcccgcaa
     2401 gaatcctctc cacctgacgc ttcaccagtc gcggcgaaag ccagatgtgg gggtctaccc
     2461 cttcgtcccc ggacccggcg cgttcgatcc cctcctccgt tcgaacgaga gtgagcgccg
     2521 ggttggccga tgagatccgg ggatacaggt tcccctcgaa cggcaccccg atggtgaacc
     2581 aggcggctga gcgggcaagc tccaccatgt cggccggcct gggctcgaac gtgtgggggt
     2641 ccgcccccct cggaatcagg acgaacacct ggacccggtt tcctcctacc cgctccacga
     2701 agtatttctg cggcgcgatg ctcacgaaga ctttcatggg acccgcggaa acgggacggt
     2761 ggagcagaaa gccgacggca aggagcggca ggaggagcag aaaaagtccg atggaccagc
     2821 gcttcattct ttggcctcct tttccagttt ccccaggcaa ttcgaacaag tcccccggaa
     2881 gacgacggac acgctttcaa ccccatgccc ctggggacag gcgggaaaat gcttccactc
     2941 ttccggcagg tagccctcca ggcagtagat ccgcccgcag cttcgacact cgaagtgcgg
     3001 gtgcgacgga ttgtgggaac acgccatctc gtaatagagc actccatcgg tcccggtgac
     3061 ttcccgggca aggttcttct cgacgaggag cgcgagaaac cggtaaaccg tggcgaggtc
     3121 ggcggttccc tcgggcaggt cctcgtgaag agcctgggcc gtaaaaagcc cgcagcccga
     3181 gtggagcgtc tccagcatga ggactctcgc cggggtgacc cgggcgccga tcgatttgag
     3241 tattttggcc gcctggcaag cgttttccat ggaagggatc atagcctatt tgcaaatcgt
     3301 ttgcaagtaa acctccaggt cgtccgactt cgggcggaaa cgggtaaaat gatcaggact
     3361 ccaagggaag gaaccaggaa aacgatgacc ccactcgaac ttttccgcat gtttttcaaa
     3421 atcagcgccg tcaccttcgg cggagggatc gccatccttg cgatggcgaa aacagagctg
     3481 agaaaacggg ggttccccga ggagcagatg gccgacatcg ccgcattggc gacctcgctg
     3541 ccgggcccca ttgccaccag caccgcctgg ctggcaggga gaaaaatcgc cggctttccc
     3601 ggcgctttcg cctccgtcgc gggaatcctc cttccgccgt ttttcgccat cctgctcctg
     3661 tccgggtttg tcctggctca ccgggattcc gccctgcttt cggccttttt caagggggtt
     3721 ctctgcgccg tggcggccct cgtcgcaacc ctggtctacg gggaaatccg ccggacgttc
     3781 acgggaaagg gacgctggct caacctcctt ccctacgccc tggtgatcgc cctgatcctg
     3841 tgggcaggac tgcatccgct ggcggccatg gcctgcggcg ttggcctgcg gctccttctc
     3901 ccggagggaa cccgatggag ctgctgaaac tcgcgtgggg cttcgcccag gtcggtatcg
     3961 gggctttcgg aggggggatc tccacgctcc ccctgatccg ccatatcctg gtggacaact
     4021 acgggtggct ctccctcgac gggttcgacc aggtcctcgc cctggcgcag gtgaccccag
     4081 gccccatcgc cgtcaacgcc gccaccttcg tgggctttca gcgggcagga ctgaccggcg
     4141 caaccgtggc caccctctcc gtggtcgtcg cgccgctttt aatcgtcatc gccgttgccg
     4201 cactcctgca gtgcggatcg aaggactgca tggaccgttt ccggaacgcc cttcgcccca
     4261 ccgtggccgc cctgctgacg ctggccttcc tgcccctcgc ccggacggcc ttctcgggaa
     4321 tcggcacaat cgccctcttc gcggccggca tcggggcctt aaaccttcgc ttcttcaggg
     4381 aaaacccgcc cgcccttttc ctgctgtccg gcctggccgg acttttcctc ttcctctagt
     4441 tcgggttgcg gtcggcgacg atcaccgcga tcatctcaag gggttcggaa ccggtgttct
     4501 tcaggccgtg ggtgtgcccc tggagggcaa tgaacacgtc acccggcccc gcaggaacct
     4561 ccgtcccgtc ctcgctgtag attccctctc ccgaaaggat ccagtaaacc tcctcgttct
     4621 ccccgtggag gtgcggcccg atgcttgagc ccggatccag ctcgatccgg gaaaccatct
     4681 ggaagcggct gccgggcatc ttttcccctg gcggcagcgc gtgggtcgcg taccccccgc
     4741 cgaccccgtt ggtgaaggct tccctgcgga ctttttccag ttctgaggct ttgcggatca
     4801 tctcgactcc tccttttgca tggtcgtttt tctgtcgtat ttcctatccg ttccggaggc
     4861 cgttccaggc ctccccccag gacatcccga acacgaagct ctccaggggt ttccgctcgc
     4921 gtggagccag ttcgcgctct ttcaggtctt cgggaaggtc ttccgggtcc cccgggtagc
     4981 ccacggcaat cgccacgagg gggtcgtgcc cctccggaac gccgcaggtt tccctcacct
     5041 ttgccgcatc gaagcctgcc atggcatggg cgaacatccc catggcaacc gcctggagca
     5101 tcaggttctc caccgccagc cccacatcgt ggcccgcgtg gcggttgggc ttgccgttgt
     5161 gatcgaaggc cgccttggcc gaaaccacca tcagcacgga ggcccggcct gcccagattc
     5221 ggttggcttc cctcaggcag ccgagaaggg tctcgaacgc ctttggctcc tccttcgtcg
     5281 ccacgatgaa gctccatggc tgctcgttga agcaggacgg ggcccaacgc gccgcctcca
     5341 gcaggctgag gaccgcttcc cgggagacgg gcttttcgga aaaagccctc gggctccatc
     5401 ttcgagcgat gagatcgtcg atcgggtgat cgggtgacgc ctgtttttcc accgtggttc
     5461 tccccctttc ttcagccgtt tcccaagaga aattgtaaca gcagaaaact tctcccaagc
     5521 tgatcttccc gcccggaaaa ggcgaagggc cgcctcctcg aggccttgct ttaagaccgt
     5581 ttcggcccgg aagccccgtt tttccgcggg cgagtccctg ggcagccttt ttttgccgcc
     5641 ttcccctttt ggtctgggaa aacccacgaa aaaggggggc ttgcgccccc cggttggttc
     5701 cctgttcgaa aagcttttcc ttcccgccgt ttagtccggg aaaacgggaa gcctctccag
     5761 gtagaccagg tctgccccgt cgtaaccgcc ttcctcgagc agcaccgcgg tctttgccac
     5821 caccttcgcg ccggccgcag acagcagttt ctccaccgac cgcagggacc cgcccgtgga
     5881 aaccacgtcg tccaccacgc agacccgcct tccttccacc ttggcccggt cgaccccgtc
     5941 catgacgaga agctgcttcc cggccgtcgt gatggattgg acttccacca ccaggggatc
     6001 ttccatgtag cttttcacgg acttgcgcac cgtgacgtag tcgaccccca ggcggaccgc
     6061 cagcgcgtgg gtcagtggaa tgcccttcgc ctccaggcag accagcatct cgatcgtgtc
     6121 cttcggcaga agcggcagca gggcgtccgc gcaggcctcg atcagcttcg tgtctcccag
     6181 catgacgaac gaggcgatca ccaggtgctc tgccacccgc accttcttca ggcggcgggt
     6241 gaggcccgct actttcaatt catagaaatc cgttccgttt ccagccattg ccggggcccc
     6301 ctagaggtga aagatcagct tgatggccag gccggccaga agcccgtaaa aggggttcca
     6361 ccgggccgaa accagcgtcg tggcgccgat gaccatcccc cagggagaga aaccgtaggg
     6421 gcccgcgaag gccggggtgc tggcgatggc tccctggatg ttcgtcgcga aggtcacgaa
     6481 cgttcccagg atgaacaaaa agcccgcgat cgaggaacgg tgaacgtaac gcccgatcac
     6541 cggcagcagg cggaaaagca ggatcgccgc catgatgccc atcatcatca cggaagaccg
     6601 cagcgggttc ggagccgtgg ccgttccgga gatgatggcc tcgaccgggc cgccgccgaa
     6661 gagggtcgac aggatatcgg ccaggctcga gtaaatcgcc aggtggtcga tgttcgtgtt
     6721 cacgttggcg atgctgcccg tgatcttgcc gaaggagatg ttcgccccga tgttcaggca
     6781 ggcgatgccc agggccccga ggaaaaccgc ccggttgcgc cagaagcacc actcgatgtt
     6841 cccgaagtgg aagcgctcgt tctccatgtg gatctccggc gcgtcgattt ctttcttcgc
     6901 cacgtagcgc aggtagttgt agaccgccgt ggaagccagc accgacacga tgatcgtctt
     6961 ggccaggtcc ttcgtgatga accagaccgc cagggccgaa acgaaggaga ccgcaccgga
     7021 gaacttctcg gaggcgtaca ggttcaccgc cacgaaggcc agcatgatg
//
LOCUS       DAOOVN010000040         6613 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_6806_length_6613_cov_14.213022, whole genome shotgun sequence.
ACCESSION   DAOOVN010000040 DAOOVN010000000
VERSION     DAOOVN010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6613
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6806_length_6613_cov_14.213022"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..1290
                     /locus_tag="PLY83_04655"
     CDS             67..1290
                     /locus_tag="PLY83_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOV29356.1"
                     /translation="MNPGISRRSLGVPASSIRGLIPFAERALKAGKKVHFLNIGQPDV
                     PTPEGYFEAVRSFREPVLAYGGSHGNPKLVQSLQRVYREELGVALAPENILVTAGGSE
                     ALWFTVMVLCDPGDELIVPEPFYANYAAICAGAGVRIVPVTTRTEDGYHLPVSDPARA
                     VESKITPRTRGILLSHPGNPTGTVFTRQEVELLGSIASRFGLFLVADEVYRDFTYDGR
                     PFTSFASLEEAKEHVVVIDSVSKRYSACGARIGFIVSRNEAVMEQAMKFAQSRGCTPT
                     LEQIGAAALYDTPSSFLEGVRREYQMRRDALCHALETIPGVTLRKPEGAFYLSARIPV
                     QDAQAFAIWMMEEFDLNGETVLFAPLEGFYATPGLGRNEIRLCYALEKAKLERAAEII
                     RAGLQAYGNRKGGLA"
     gene            1287..2132
                     /locus_tag="PLY83_04660"
     CDS             1287..2132
                     /locus_tag="PLY83_04660"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOV29357.1"
                     /translation="MKGERKEQILNSVLRTFDVLELLAKRGELGVTRIAEEVGLDKST
                     VYRILATLKSIGYVCQNPVTQGYANTPRFALLSPRTPGAEELRERAHPFLSRLAEKTG
                     ETASLGILEGRDILYIDTVQCSAMIQVNLPIGQKLPAYCSALGKAILAFLPEERVREL
                     YGGADLERFTPNTLSSCDALAGELAAVRLLGFARDQEESHLQLACLGVPVFGADGRIA
                     AGISLSYPCFRHPDLKQAERLLLSPLREAGIAVSKALGFKGSYPGRSQKNDDQRNTRK
                     HPLKE"
     gene            2138..3082
                     /locus_tag="PLY83_04665"
     CDS             2138..3082
                     /locus_tag="PLY83_04665"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOV29358.1"
                     /translation="MPLSLDKVSELMEKAGIPGRDLYDRPASTKRFPDGGHYRIEMSG
                     VEGPKVLEALIAERRRRDIPIHRLVSFCQGGTLFDDAELRDFAQMAAEDKMEVIAIPG
                     PRNAWDIGRQFVTPDGQRCGGLNHRGSDEIRKVIADMFRMYEIGFRGFMLVDRGVLAL
                     VKTMQEQGSFPKDIAIKLSVWAGVSSPAGAKLAQDLGASSFNPVSDLTLPQMAAIRKV
                     VNIPIDFYIWTFDSYGGANRLYDAPEVAKIYSPCYFKFEPAPTAGFYNPFNSEEEHVR
                     LMEKKVKWAEWVIHHVAANEPDVRLSPHGASDLFVPKV"
     gene            complement(3153..4250)
                     /locus_tag="PLY83_04670"
     CDS             complement(3153..4250)
                     /locus_tag="PLY83_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOV29359.1"
                     /translation="MLKDTLQPLSQRLQREGLDALLLGPSSDLEYLAGLRLFADERFK
                     GIVLLSDGRLFSIVPRLYREQMEAVLGPECPQALWDDAEGFLEKVTALWKDFGLEGKT
                     VAVNDGVRAVDLLDLEAAVPGTRFVNGHRIVEAMRLIKSPEEMDVLRHAARIADEAFS
                     ALLPFIRPGVREGEVARKLEDLLVEKGADGLSFPTIVASGPNGSNPHYMGKSRVLCEG
                     DPVVIDFGCTFQGYCSDTTRTVFVGKASDEDRALYRVVLEANLAGEAAAREGATAQDV
                     DRATRSVIEKAGYGKYFINRTGHGIGVAVHEAPYIMEGNLQVLEKGMAFSVEPGIYIP
                     GRLGIRIEDIVLVTDRGPEPLSHFTKEITIV"
     gene            complement(4319..5512)
                     /locus_tag="PLY83_04675"
     CDS             complement(4319..5512)
                     /locus_tag="PLY83_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HOV29360.1"
                     /translation="MRKSWKVLLAGLFLFGLMAGTAMAEAPFHIGVVTLTVSQAEDTY
                     RGAERLIKEYGDVAKGGYIKHITLPDSFMSEMETSISQIAGLADDPKMKVIVVDDAVP
                     GTTEGFRRVKEKRPDILCFAGEPQEDPNVITSVADLAIGVDNVLRGYLIIHTAKRMGA
                     DTFVHISFPRHMSYELLSRRRNIMEQACKDLGLKFVFETAPDPTSDVGVAGAQQYILE
                     KVPAWVQKYGKKAAFFCTNDAQTEPLLRQVAKYGAFFVEPDLPSPLMGYPGAFGIDLS
                     KEKGNWPAILKKVEQVVIKNGGKGRMGTWAYSYGYATTATLGEYGKRIVEKKAKLGNM
                     KDLMDAYEKFTPGAKWNGSNYVDLGTGVKKKNFILVYQDTYVFGKGFMGATSVKVPEK
                     YLKMK"
     gene            complement(5669..6340)
                     /locus_tag="PLY83_04680"
     CDS             complement(5669..6340)
                     /locus_tag="PLY83_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOV29361.1"
                     /translation="MEEFTDNLKSLREKVYQYLKKGLNEGRLRQGAFLNLNAISAELG
                     MSRTPLRDALFQLEAEGYVTIFPRRGVMVNRLDLEKIRNIYEISGALESTVVAHSGHK
                     LTARDIETLESLNAQMKEAVETQDIDGYYDKNTAFHSVYLSLSQNTDLIRTVQTMRER
                     LYDFPRSKAFIKDWELTAIGEHAEFIRRLKAKDFNGAAEFLRDVHWSFEVQERYARLY
                     YFGQE"
BASE COUNT         1282 a         2066 c         1963 g         1302 t
ORIGIN      
        1 acatcctgaa gggaaaagag gaagaattcg cccgcatgct cgaggagttc gagtaggagg
       61 ccccctgtga atccaggaat ctcgagaaga agccttggcg tcccggcctc gtcgatccgg
      121 gggctgatcc ccttcgcgga gcgggccctg aaggcaggga aaaaggtgca tttcctcaac
      181 atcggccagc ccgacgtccc gacacccgag ggctatttcg aagccgtccg ctctttccgc
      241 gaacccgtcc tggcctacgg cggctctcac gggaacccga agctggtgca gtcgctgcag
      301 cgcgtctacc gggaggagct tggggttgcc ctggcaccgg agaacatttt agtgaccgcc
      361 ggcgggagcg aggcgttgtg gtttaccgtc atggtcctgt gcgaccccgg ggacgagctg
      421 atcgtaccgg agccgttcta cgcgaactac gccgccatct gcgcgggagc cggggtgagg
      481 atcgtgcccg tgaccacccg aacggaagac ggctatcacc ttccggtctc agacccggcc
      541 cgggccgtgg agtcgaaaat cacaccccgg accaggggaa tccttctgtc ccacccaggc
      601 aaccccactg gaacggtttt cacccggcag gaagtggaac tcctgggaag catcgcgtcc
      661 cggttcggcc tttttcttgt cgccgacgag gtttaccggg actttaccta tgacggccgc
      721 ccgttcacca gcttcgcttc gctggaggaa gcgaaggagc acgtcgtggt gatcgactcc
      781 gtttccaagc gctacagtgc ctgcggagcc cggatcgggt tcatcgtttc ccggaacgag
      841 gcggtgatgg aacaggcgat gaaattcgcc cagtccaggg ggtgcactcc caccctggag
      901 cagatcggcg ctgcggcgct ctatgacact ccgtcctcct tcctggaagg ggtccggagg
      961 gaataccaga tgcggcgcga cgccctgtgc cacgccctcg aaaccatccc gggtgttacc
     1021 ctgagaaagc ccgagggggc cttttacctt tccgcccgaa tccctgtcca agatgcccag
     1081 gcctttgcaa tctggatgat ggaggaattc gaccttaacg gggaaacggt tcttttcgcg
     1141 cccctcgagg ggttctacgc cacgcccggg ctggggcgca atgaaatccg gctctgctat
     1201 gccctcgaaa aggcgaaact ggagagagcg gccgaaatca tccgggcggg gctgcaggca
     1261 tacgggaacc ggaaaggcgg gcttgcatga agggcgaaag aaaggagcag atcctgaatt
     1321 cggttctgcg gactttcgat gtgctcgagc ttctggccaa aagaggagag ctcggggtta
     1381 cacggatcgc cgaggaggtg ggactggaca agagcaccgt gtaccgcatc ctggctacgc
     1441 tgaaatcgat cgggtacgtc tgccagaacc ccgtgaccca gggatacgcc aacacgcccc
     1501 ggtttgccct gctttccccg agaacgcccg gcgcggagga gctgcgtgaa cgggcgcatc
     1561 ctttcctttc caggctggcc gaaaaaacgg gtgaaaccgc cagcctcggc atcctggaag
     1621 gcagggacat cctgtacatc gacaccgtcc agtgttccgc catgatccag gtcaaccttc
     1681 cgatcgggca gaagctcccg gcctattgct cggccctggg gaaggccatc ctcgccttcc
     1741 tgcccgagga gagggttcgg gaactgtatg gcggagccga cctggaacgg ttcaccccca
     1801 acacgctttc ctcctgcgac gccctggccg gggaactcgc ggcagtcagg ctcctgggct
     1861 tcgcccggga ccaggaagaa tcccatctcc agctggcctg cctgggggtc ccggtcttcg
     1921 gggccgatgg aaggatcgcg gcggggatca gcctttccta tccctgcttc aggcaccccg
     1981 acctaaaaca ggcggaaagg ctcctgcttt ccccgcttcg tgaagccggg attgctgttt
     2041 cgaaggccct ggggttcaag gggagctatc cgggccgatc gcagaaaaat gacgatcaaa
     2101 ggaacactcg aaaacaccca ttaaaggagt gattggaatg cccctcagtc tcgacaaggt
     2161 ctctgaattg atggaaaagg ccgggattcc cggccgcgac ctctacgacc gtcctgccag
     2221 cacgaagcgt ttccccgatg gagggcacta ccggatcgag atgtcaggtg tcgaagggcc
     2281 caaggtcctc gaagccctga tcgcggaacg ccgtcgaagg gatatcccca tccaccgcct
     2341 cgtatccttc tgccaggggg ggacactctt cgatgacgct gagcttcggg atttcgccca
     2401 gatggccgca gaagacaaaa tggaggtcat cgcgatccct ggcccccgga acgcgtggga
     2461 catcgggcgg cagttcgtga ccccagacgg ccagcgctgc ggggggctga accaccgagg
     2521 ttcggacgag atccggaaag tcatcgcgga catgttccgg atgtacgaga tcggttttcg
     2581 cgggttcatg ctcgtggacc ggggcgtcct ggccctcgtg aaaaccatgc aggagcaggg
     2641 cagcttcccg aaggacatcg ccatcaagct ctcggtctgg gccggggttt cctcgccagc
     2701 gggcgcgaag ctggcccagg accttggagc gagttccttc aaccccgttt ccgacctgac
     2761 gctgccgcag atggccgcga tccggaaggt cgtgaacatc ccgatcgatt tctacatctg
     2821 gaccttcgac tcgtacgggg gggcaaaccg cctctatgac gcgcccgaag tggcgaagat
     2881 ctacagcccc tgctatttca agttcgaacc cgcccccacc gccggtttct acaacccctt
     2941 caactcggaa gaagagcacg tgcggctgat ggaaaagaag gtcaagtggg cagaatgggt
     3001 gatccaccac gttgcggcca acgaaccgga cgtccggctc tctccgcacg gggcgtcgga
     3061 cctgttcgtg ccgaaagttt aaaaagggaa agccaggcgg gggagagaac actttcccgc
     3121 ctggctttcc gaatcctgca tcgaatgaga aatcagacga tggtgatttc tttcgtgaag
     3181 tggctcaggg gttccggccc cctgtctgtc acgaggacga tatcctcgat gcggatgccc
     3241 aggcgcccgg gaatgtagat gccgggctcc acgctgaagg ccatcccctt ttcaaggacc
     3301 tggaggtttc cctccatgat gtacggggcc tcgtgaacgg ccactccgat cccgtgcccg
     3361 gtccggttga tgaagtattt cccgtagccc gctttttcga tgaccgatct cgttgcccgg
     3421 tccacgtcct gcgcagtcgc cccttcccgc gcggccgcct ccccggcgag gtttgcctca
     3481 agaacaaccc ggtagagggc ccggtcctca tcggaagcct ttccgacgaa gacggtgcgg
     3541 gtcgtatcgg agcagtatcc ctggaacgtg cagccgaaat cgatgaccac ggggtcgcct
     3601 tcgcagagca cccggctttt ccccatgtag tggggattgg aaccgttcgg cccggaagcc
     3661 acgatcgtgg ggaaggacag tccgtccgcc cccttctcga ccagcaggtc ctccagcttt
     3721 cgggctacct ccccctcccg cactcccgga cggataaaag gaagaagggc agagaaggcc
     3781 tcgtcggcaa tgcgggccgc gtgccgaagc acgtccattt cctcggggga cttgatcagc
     3841 cgcatggctt ccacgatgcg atgtccgttg acaaacctcg tccccgggac cgcagcctcg
     3901 aggtccagga ggtccactgc ccggacgccg tcgttcacgg ccacggtttt cccctcaagt
     3961 ccgaagtctt tccacagggc cgtgaccttt tccaggaagc cctcggcatc gtcccagaga
     4021 gcctgggggc actcgggccc cagaacggcc tccatctgtt cgcggtaaag cctggggacg
     4081 atggagaaaa gccggccgtc ggaaagcaag acaatcccct tgaagcgttc gtcggcgaaa
     4141 aggcgaagcc cagcgaggta ttccaggtcg gaagagggtc ccagcaggag cgcatcgagc
     4201 ccctcccgct gcagccgctg gctgagcggc tgcaaggtgt ctttaagcaa gaaagtcgcc
     4261 tccttcatga cgggagccgc gcccccgaaa aaaagggggg aagaaatccc cccggcacct
     4321 acttcatctt caggtatttc tcaggcacct tcacgctcgt ggcacccatg aagcccttgc
     4381 cgaacacgta ggtgtcctgg tagacaagga tgaagttctt cttcttgacc ccggtcccca
     4441 agtccacata attgctgccg ttccacttcg cgcccggggt gaacttctcg taggcgtcca
     4501 tcaggtcctt catgttgccg agcttcgcct tcttttcgac gatgcgcttg ccatactcgc
     4561 ccagcgtcgc ggtcgtggcg tagccgtaag agtaggccca ggtgcccatg cggcccttgc
     4621 ccccgttctt gatgacgacc tgctcgacct tcttcaggat ggcgggccag ttgccctttt
     4681 ccttggagag atcgatcccg aaggctccgg gataccccat cagcggggag ggaaggtcag
     4741 gttccacgaa gaaggcgccg tacttggcaa cctgccggag caaaggctcg gtctgggcgt
     4801 cgttggtgca gaagaaggcg gccttcttgc cgtatttctg gacccatgcg ggaaccttct
     4861 ccaggatgta ctgctgggcc ccggcaaccc ccacgtcgct cgtcgggtca ggagcggtct
     4921 cgaaaacgaa cttcaggccc aggtccttgc aggcctgctc catgatgttg cgcctccggg
     4981 aaaggagttc gtagctcatg tggcggggga aggaaatgtg gacgaaggtg tcggcaccca
     5041 ttctcttcgc ggtatggatg atgaggtagc cgcgcaggac gttgtccacg ccgatggcca
     5101 ggtctgccac ggaggtgatg acgttcgggt cttcctgggg ttcaccggcg aagcagagga
     5161 tgtcgggccg tttctccttg acccggcgga agccctcggt ggttccggga accgcgtcat
     5221 ccacgacgat gaccttcatc ttgggatcgt ctgcaagtcc ggcgatctgg ctgatgctgg
     5281 tctccatttc cgacatgaag ctgtcgggca gggtgatgtg cttgatgtac cctcccttgg
     5341 ccacgtcgcc gtattccttg atcagccgct ccgcaccgcg gtaggtgtct tccgcctgtg
     5401 acaccgtcag cgtgaccacg ccgatgtgaa acggcgcttc cgccattgcc gtccccgcca
     5461 tcaggccgaa aaggaacaaa cctgccagca ggactttcca ggacttcctc attctccgaa
     5521 cccccttcat aaaaatatga ctaaaatacg gttgatatat tagattgccg aaccggaaga
     5581 tgtcaatctt gcacacaaaa agccgggcag ggaggatcct gcccgggcac cggtttccct
     5641 tggggccttc cgaaatcttg atcccctttt actcctggcc gaagtaatac agcctggcat
     5701 agcgttcctg gacctcaaag gaccagtgga cgtcccgcag gaattccgcc gcgccgttga
     5761 agtcttttgc cttcagccgc cggatgaact ctgcgtgctc cccgatcgcc gtcagttccc
     5821 aatccttgat gaacgccttg ctccggggaa aatcgtacaa cctttccctc atcgtctgga
     5881 ccgtccggat caggtccgtg ttctgggaga ggctgagata cacggagtgg aacgccgtgt
     5941 tcttgtcgta ataaccgtcg atgtcctggg tttccacggc ttccttcatc tgggcgttca
     6001 ggctttccag ggtctcgatg tcccttgcgg tgagcttgtg ccccgaatgg gcgacgaccg
     6061 tgctttccag ggccccgctg atctcgtaga tgtttcggat cttttccagg tccaggcggt
     6121 ttaccatcac cccgcgcctg ggaaagatcg tcacgtaccc ctccgcttca agctggaaaa
     6181 gagcatccct cagcggggtc cggctcatcc ccagttcggc gctgatggcg ttcaggttca
     6241 aaaaggcacc ttgcctgaga cgcccttcgt tcaacccttt cttcaggtac tggtagacct
     6301 tctcacgcag ggacttgaga ttgtccgtga actcttccat gggaacgcct ccgcagacac
     6361 ttcagaatgc agccattaaa ccgcaaaaag ggtcgaatcc gcaatgccgc tcttccatga
     6421 tgaactcttg cgctgatttt cgaacgcttt agaataaaca atcaaatccg ccaggaggag
     6481 gtccttgccg ctccatgaaa ctctcgcagg ttgtccaaac cctcgaatcc gccggctttg
     6541 ccccggcctt tgcccgggaa cccctcgccg acccagaact ctccgatgtc tgcacccgga
     6601 cggaacaggt ttc
//
LOCUS       DAOOVN010000041         5626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_8145_length_5626_cov_16.141088, whole genome shotgun sequence.
ACCESSION   DAOOVN010000041 DAOOVN010000000
VERSION     DAOOVN010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5626
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8145_length_5626_cov_16.141088"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="PLY83_04685"
     CDS             <1..465
                     /locus_tag="PLY83_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOV29362.1"
                     /translation="IDTERKRIGLGYKQLNDPWNGVEERYRPKQDVPVKVVRLADFGA
                     FVQLEEGVEGLIHISQLSRQRVEKPGDVLQEGQEIVARILEVNPAERRIRLSLSAIED
                     EQERKHREEERKTRDEERRKRGPEPRKTHEPAYQPDDTSVTIGDVLRDILDS"
     gene            543..1046
                     /gene="def"
                     /locus_tag="PLY83_04690"
     CDS             543..1046
                     /gene="def"
                     /locus_tag="PLY83_04690"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOV29363.1"
                     /translation="MSLLKVLVYPDPALREPSKEVERFDQELGNFIRDLWETMLAYDG
                     VGLAATQVGKNLRVCVISWKEHRLILVNPRITSTEGEQAGEEGCLSFPGIFEKIRRPL
                     KVRVEAVDEAGKPFSLEADGFLARALCHEIDHLDGRLMIDHLSPLKREIVRKKLLKRS
                     KEERRKP"
     gene            1043..1969
                     /gene="fmt"
                     /locus_tag="PLY83_04695"
     CDS             1043..1969
                     /gene="fmt"
                     /locus_tag="PLY83_04695"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOV29364.1"
                     /translation="MNVWFFGSGHFAALCFREIAPHLRFEKVVTLPPSPAGRGRALRP
                     TPLEEQARSLGFSPIHSSQPSADPVLREDILANPPDLALIVDFSRFIREPLLNGPRMG
                     CLNIHPSLLPAYRGAAPIQRALMDGATQTGVTLFRLVEKMDAGPILLQKAVPVGLETT
                     AGELSEILAREGSQIFLKSLEYLRGGYAEYIAQDESAATFAPKISKTEERLAWSDPAP
                     VVHNRVRALNPSPGVHCFFSGKRLKIWKTRSIDGPCANPGRITGFEQGFPVVACSSGG
                     VVLLEVQPEGRGILRGDEWARGSRIKEGDQFE"
     gene            1966..2703
                     /locus_tag="PLY83_04700"
     CDS             1966..2703
                     /locus_tag="PLY83_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29365.1"
                     /translation="MSVAKRKGHDLLTGLGGFSWEKVIAVVGALGSGKSEWLLNLANA
                     FLDREETVTVADVDIINPYFCIREVSSLLKKMGLSVILPFKGTQWSDLPSISPEVDRA
                     FDAPGRLLIDIGGDPRGAMALTQFAPRMEQAGYNLLAIVNAYRPQTSTVEGIEAMIRS
                     IERVSGLKVSAVISNSHMMSETTLDDVLQGYETALAAAEKMGLPILFVGVPESAMKEA
                     ERRFEGRKVNLWPVSRYILLPWERGGM"
     gene            2703..2936
                     /locus_tag="PLY83_04705"
     CDS             2703..2936
                     /locus_tag="PLY83_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOV29366.1"
                     /translation="MAKGRVGVNEERCKSCGLCVAACPMKVLRISDKINKKGYRPVEQ
                     FKEGCIACRMCAITCPDVVLEVFRLEDDKACNA"
     gene            2953..4014
                     /locus_tag="PLY83_04710"
     CDS             2953..4014
                     /locus_tag="PLY83_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOV29367.1"
                     /translation="MGKRFLMKGNEALAEAAIHAGCKYFFGYPITPQNEIPEYMSKKL
                     PEIGGVYLQAESEVAAVNMIFGAAGTGKRVMTSSSSPGISLMSEGISYIAGAELPCVL
                     MNVMRGGPGLGGILPSQSDYFQATRGGGHGDYRLLVLAPSTVQEAVDLTMLAFDLAEK
                     YRNPVMVLADGFMGQMMEAVEFKETRSEGLPDPAEWAVGYMKQRGSRALIKSLYLDPE
                     ELEAHNWKLQAKYAQMAQHEKRFEGYLMDDAEILIAAYGTVARIARTAINELRGEGVK
                     VGLFRPITLFPFPYEPLENLPASVRQILTVEMSCGQMVEDVRLAVGKERPVSMYGRVG
                     GMTPSVDEIKDQVRKALGR"
     gene            4027..4770
                     /locus_tag="PLY83_04715"
     CDS             4027..4770
                     /locus_tag="PLY83_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOV29368.1"
                     /translation="MKETKIFEKPKSMIDVTTHYCPGCTHGIAHRLIAEVIDELQIQE
                     RTVAVAPVGCSVFLYNYINVDSYEAAHGRAPAVASACKRLLPDSVVFSYQGDGDLASI
                     GMAEIVHAANRGEKITVIFINNAIYGMTGGQMAPTTLVGQKATTCPLGRDPKLAGYPI
                     RMCEMLATLQAPAYITRVSVAKPKYTIKAKEALTKAFQTQIEGKGFSFVEILSTCPTN
                     WGMRPVDATNWLEEHMMPEFPLGVFKTPE"
     gene            4787..5347
                     /locus_tag="PLY83_04720"
     CDS             4787..5347
                     /locus_tag="PLY83_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOV29369.1"
                     /translation="MAKFLRGLIAAGFGGQGVLLLGQIVAHAAMKEGRYVTWIPSYGP
                     EMRGGTANCSVAISDEEIGSPVVPHPDCLVIMNQPSLAKFEPDLKSGGTLLYNSDLVT
                     YPQPRTDIRVVPVPCNSIALELGNEKVANVVTLGALAAASDLVSVEGCKETLAELMGK
                     KKAALVEINMTAFDRGVEIARKALGQ"
     gene            5355..>5626
                     /locus_tag="PLY83_04725"
     CDS             5355..>5626
                     /locus_tag="PLY83_04725"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOV29370.1"
                     /translation="MAGDWSVLHSRKLYEGRILNLRLDEVLLPSGRLAVREIVEHLPA
                     VAMLPVADDGSVLLIRQYRDAIRQEILEIPAGILNPGESPRDAARR"
BASE COUNT         1229 a         1670 c         1716 g         1011 t
ORIGIN      
        1 atcgacacgg agcgcaagcg gatcggcctc ggctacaagc agctgaacga tccctggaac
       61 ggggtggagg aacggtacag gccgaagcag gacgttcccg tcaaggtcgt ccgcctcgcg
      121 gatttcggtg ctttcgtcca gcttgaggag ggggtcgagg ggctgatcca catctcccag
      181 ctgagccgcc agcgagtcga aaaaccggga gatgtcctcc aggaggggca ggaaatcgtc
      241 gcccgcatcc tggaagtcaa cccggctgaa cggcggatcc gcctaagcct gagcgccatc
      301 gaggacgagc aggagaggaa gcatcgcgag gaggaacgca agacccgcga cgaggaacgc
      361 cgcaagcgcg gccctgaacc ccggaagaca catgaaccgg cctaccagcc cgacgacacc
      421 tccgtcacga tcggagatgt tctgagggat attctcgatt cctgaaatat tgtcttataa
      481 tgttgtaaac gccggccgca acaggagcgg ccggcgtgcc tctgttaaaa aggaggaatc
      541 ccatgagcct tctgaaagtc ctggtctacc cggatccggc cctccgtgaa ccctcgaagg
      601 aagtggaacg gttcgatcaa gagctgggga atttcatccg ggacctttgg gaaaccatgc
      661 tggcctacga cggggtcggc ctggccgcca cgcaggtggg caaaaacctg cgcgtctgcg
      721 tcatctcctg gaaagagcat cgcctgatcc tggtgaaccc cagaattaca agcacggaag
      781 gagagcaggc aggagaggaa ggatgcctga gctttccggg aattttcgag aagatccgga
      841 gacccctgaa ggttcgagtc gaagccgtcg acgaggcggg aaagcccttt tcactcgaag
      901 ccgacggctt tctggccagg gcgctctgcc acgaaatcga tcacctggac ggacgcctga
      961 tgatcgacca cctcagcccc ctcaagcggg aaatcgtgag gaaaaagctt ctcaaacgtt
     1021 cgaaggagga gaggaggaag ccgtgaacgt ctggtttttc ggctccggtc atttcgcggc
     1081 tctctgtttc cgggagatcg cacctcacct gcgctttgaa aaggtcgtca cccttcctcc
     1141 gtccccggct ggaagaggaa gggcccttcg gcctaccccc ctcgaggagc aggcccgtag
     1201 cctcggcttc tccccgatcc attcttctca accctccgcg gatcctgtcc tccgggaaga
     1261 catcctggca aatccacccg atcttgcctt gatcgtcgac ttttcccgtt tcatccgcga
     1321 gcccttgctc aacggcccac gcatgggatg tcttaacatc cacccttccc ttcttcccgc
     1381 ctaccgcgga gccgccccga tccagcgtgc actcatggat ggggcaacgc agacaggtgt
     1441 aacgctgttc cgactcgtgg agaaaatgga cgcgggcccg atcctcctgc agaaagcggt
     1501 ccccgtgggg ctggaaacga cagccggcga actgtcggaa attctggcgc gggagggtag
     1561 ccagattttt ttaaaaagct tagaatatct ccgtggcggt tatgcagaat acattgcgca
     1621 ggatgagagt gccgcgactt tcgctcccaa gatcagcaaa accgaggagc gcctggcctg
     1681 gagcgatccc gcgccggtcg tgcacaaccg tgtcagggcc ctgaacccgt ccccgggagt
     1741 tcactgtttt ttttcgggaa agcgcctgaa aatttggaag actcgctcga tcgacggccc
     1801 ctgcgccaac ccgggaagga taacgggctt tgaacaggga tttcccgtgg tggcatgctc
     1861 aagcgggggg gtcgtcctgc tcgaggttca accggaaggc cggggaatcc tcaggggtga
     1921 cgagtgggcc aggggaagcc ggatcaaaga aggagaccaa ttcgaatgag cgttgccaag
     1981 aggaaggggc acgatcttct gacaggcctg ggcggttttt cctgggagaa agtgattgcc
     2041 gtcgttggag ccctcggatc cgggaaatcc gaatggcttc tgaacctcgc caatgccttt
     2101 ctcgaccgcg aggaaacggt cacggtcgcc gacgtggaca tcatcaaccc ctatttctgc
     2161 atccgggaag tttcttccct cctgaaaaaa atgggactgt cggtaatcct tcctttcaaa
     2221 ggaacacagt ggtcggatct cccgtccatt tcgccggaag tcgatcgggc gtttgacgcc
     2281 ccggggaggc tcctgatcga catcggggga gatccgaggg gagcgatggc cctgacccag
     2341 ttcgccccgc gcatggaaca ggcgggctac aaccttctgg ccatcgtcaa cgcctaccgt
     2401 ccccagacct cgacggtcga gggaatcgag gcgatgatcc ggtcgatcga gagggtcagc
     2461 ggcctgaagg tctctgccgt catcagcaac tcccacatga tgtccgagac aaccctggac
     2521 gatgtcctgc aggggtatga aacggcactc gccgcggcag agaaaatggg gcttccaatc
     2581 ctctttgtgg gagtgcccga aagcgcgatg aaagaggcgg aacgccggtt cgaaggcagg
     2641 aaggtcaacc tgtggccggt ttccagatac atcctattac cttgggagag gggtggcatg
     2701 tgatggcaaa gggacgcgtc ggggtcaacg aggaacgatg caaaagctgc ggtctctgcg
     2761 tggcagcctg tccaatgaaa gtcctcagga tctccgacaa gatcaacaaa aagggctatc
     2821 ggcctgtgga gcagttcaag gaggggtgca tcgcctgcag gatgtgcgcg atcacctgtc
     2881 cggacgtggt cctcgaagtc ttccggctgg aagacgacaa ggcctgcaat gcctagagcg
     2941 aggtgaaccc tgatgggcaa gcgtttcctg atgaaaggca acgaggcgtt agctgaagcg
     3001 gcaattcatg cgggctgcaa gtatttcttc ggatacccca tcacccccca gaacgaaatc
     3061 cccgaataca tgtccaaaaa actgccggaa atcggcggcg tataccttca ggccgaaagc
     3121 gaggtcgcag cggtcaacat gattttcggg gcggccggca ccgggaagag ggtgatgacc
     3181 tcctcttcca gcccgggcat ttccctcatg tccgagggaa tcagctacat cgcgggggcc
     3241 gaacttccct gtgtcctgat gaacgtgatg cgagggggtc caggcctggg tgggatcctg
     3301 ccctcccagt cggactactt ccaggcgacc cgtggaggcg gacatggaga ctaccgtctc
     3361 ctggtccttg cccccagcac ggtccaggaa gcggttgatc tcacgatgct ggccttcgac
     3421 cttgccgaga agtaccgcaa ccccgtgatg gtcctggcag acggtttcat gggacagatg
     3481 atggaggccg tcgaattcaa ggaaacccgc tcggaaggac ttcccgaccc ggctgaatgg
     3541 gccgtgggct acatgaagca gcgagggagc agggccctga tcaagagcct ctacctcgac
     3601 cccgaggaac tggaggccca caactggaag ctccaggcca agtacgccca gatggcccag
     3661 cacgaaaagc gctttgaagg ctatctcatg gacgacgcgg agatcttgat cgcggcctac
     3721 gggaccgtgg cccgaatcgc gcgcacggcg atcaacgaat tgcgcggaga aggcgtcaag
     3781 gtcggacttt tccgccccat cacccttttc cccttcccct acgaaccgct ggaaaacctc
     3841 ccggcctccg tccggcagat tttgaccgta gaaatgagct gcggacagat ggtggaggac
     3901 gtccggcttg cggtaggcaa ggagcgtccg gtttccatgt acggacgcgt cggaggcatg
     3961 acgccttcgg tcgacgagat caaggaccag gtccgaaagg ccctggggcg ataggggggc
     4021 ccttccatga aagaaacaaa gattttcgag aagccgaaat ccatgatcga tgtcacgacc
     4081 cattactgcc caggctgtac ccacgggatc gcacaccggc tgatcgcaga agtcatcgac
     4141 gagctgcaga tccaggaaag gaccgttgcc gtggcgcctg tcggttgctc ggtcttcctc
     4201 tacaactaca tcaatgtgga ttcctacgag gcggctcacg gccgggctcc cgccgtcgcc
     4261 tccgcctgca agcgccttct tcccgacagc gttgtctttt cctaccaggg agacggcgac
     4321 ctcgcttcca tcgggatggc tgaaatcgtg cacgcggcca accgaggaga gaagatcact
     4381 gtcatcttca tcaacaatgc catctacggg atgacggggg gacaaatggc cccaacgacg
     4441 ctggtcggcc aaaaggccac aacctgtcct ctcggcaggg acccgaagct ggcgggatac
     4501 ccgatccgca tgtgcgagat gctcgcgacc cttcaggctc ccgcatacat cacccgcgtg
     4561 agcgttgcaa aacccaagta caccatcaag gccaaggagg ccctgaccaa agcattccag
     4621 acccagatcg aagggaaagg attctccttt gtggaaatcc tctccacctg ccccacgaac
     4681 tggggcatgc gccccgtgga tgccaccaat tggctggaag agcacatgat gcccgaattc
     4741 ccgctgggcg tcttcaagac gccggaatag aagggggaga aagatcatgg ctaagtttct
     4801 gcgcggtctg atcgctgccg gatttggggg ccagggagtt ctgctcctcg gccagattgt
     4861 cgcacacgcg gcaatgaaag aagggcgcta cgtcacctgg atcccgtcct acgggccgga
     4921 aatgcgaggg ggaaccgcca actgcagcgt cgcgatcagc gatgaagaga tcggatcgcc
     4981 ggtcgttccg catccggatt gcctggtgat catgaaccag ccctccctcg cgaagttcga
     5041 gccggacctc aagagtggcg gaacccttct ttacaactcg gatttggtca cctaccccca
     5101 gccccggacg gacatccggg tcgttcccgt accctgcaac agcatcgccc tggaactcgg
     5161 gaacgaaaaa gtggcgaacg tcgtgaccct cggggcactt gccgcggcaa gcgacctggt
     5221 ttcggtcgaa ggatgcaagg aaaccctcgc cgagctgatg ggcaagaaga aggccgccct
     5281 cgtggaaatc aacatgaccg ccttcgaccg gggggtcgaa atcgcccgga aagcgctagg
     5341 ccagtagacg ggacatggca ggggattggt ctgtcctgca cagccgcaag ttgtacgaag
     5401 gacgcatcct caaccttcgg ctggacgagg tccttctgcc ctcgggcagg ctcgctgtcc
     5461 gggaaatcgt cgagcatctt ccggccgtgg cgatgctgcc ggtcgcggat gacggaagtg
     5521 tcctcctgat ccggcagtac cgggacgcca tccggcagga aatcctggaa attccggcag
     5581 ggatcctgaa tccgggcgag tcgccccgcg atgccgcccg caggga
//
LOCUS       DAOOVN010000042         5607 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_8180_length_5607_cov_13.729647, whole genome shotgun sequence.
ACCESSION   DAOOVN010000042 DAOOVN010000000
VERSION     DAOOVN010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5607
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8180_length_5607_cov_13.729647"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="PLY83_04730"
     CDS             <1..1108
                     /locus_tag="PLY83_04730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOV29371.1"
                     /translation="PLSWRRRTCHPFPGIARNREREVPVLKICIATDAWTPQVNGVVR
                     TLTETERRLRKMGHEIFLLTPERFATVPCPTYREIRLAWSLWDVDRFLRDFLPDAVHI
                     ATEGPIGFSVRAWCLREGFPFTTSFTTRFPEYVRSRFGLSAELLYQGLRWFHAPSSAV
                     MVATASLQEELQGRGFANLVRWSRGVDTSLFCPSLPRHPVRNRPVFLYVGRVAVEKNL
                     PAFLDLDLPGTKRVVGSGLDLEALRRTYPAVEFSGTLEGLDLAQAYAEADVFVFPSKT
                     DTFGIVMLEALACGTPVAAYPMTGPLDILRQNVTGVMDFDLRKACLEALSLDRAACRQ
                     DALLWSWDRCTRQFLENLRPRSFSKLVHDPVGGM"
     gene            1187..2332
                     /locus_tag="PLY83_04735"
     CDS             1187..2332
                     /locus_tag="PLY83_04735"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOV29372.1"
                     /translation="MKPVLLAYSGPGQGHKMAAMALREAFLAEKAPVVCLDTLTTAMP
                     FFRFLYAGLYKGMARHAHTACEQVYRLTDRVRGKSPVVRAIDWWSLQNTTVFQALAKT
                     LAPETAVCTHFLPMALLSLMRERGEFNGQIHVAITDYDLHGFWVDPGVDRYYAASVQV
                     RDRLAARGIPDSRIAVTGIPVRESFTRLALSPRIPRPLPLRILFLAHSIARETALSII
                     DSLSSIGPSIRLTVVGERELSKNRPKASICWFERIENLAPLMADADLLVTKPGGLVCS
                     EALSAGLPMLFISPIPLQETLNAQYLQGQGAGIFCESPAALAGEVRGILADPGILRRM
                     GHACQRMSAPDAARAIARRVLGRRPQVPEWEAPAAFCHPGGNRPCFP"
     gene            2320..3204
                     /locus_tag="PLY83_04740"
     CDS             2320..3204
                     /locus_tag="PLY83_04740"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOV29373.1"
                     /translation="MLSVRTAFLSDLHLGSRWCRAAELSAFLDLLECETLFLVGDIVD
                     GWSLKRRFRWPDAHTEVLAKILDFSRNSRVVYVTGNHDAFLDRLAGRKLGNIEIRLCA
                     IHETVRGTRLAVFHGDEVDRVSSEQPWLAHLGDFAYTAALWANRGANVLRRGFGLEYR
                     SYSRILKQKVKEAVNYVSGFEKKLAEEARRLGTDGVICGHIHKPAWEESRGEVFYGNC
                     GDWVESCSALVEKHDGEMEVLDWETLKQTAGVPVVTQNGEMVYRFPLPEAASSWAGAL
                     LSMNVGPTDLRDVLKTRR"
     gene            3201..5069
                     /locus_tag="PLY83_04745"
     CDS             3201..5069
                     /locus_tag="PLY83_04745"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HOV29374.1"
                     /translation="MTLDPAFLLEQILSEKAVRIAFQPIVDLSDGSVFGHEALARGKG
                     ALEMPAALFPCARTLGKTWELEKTCRENALAAMGKGNSPLQPCRLFLNASPRVFADPR
                     FPEVLAREIGRIPGISRDQIVVELTEQEGLEDESLLKFQARHLSGHGFSVALDDFGAG
                     NSGLRILMECCPAFLKLDRGIIRGIDRSALRMQLVKSLVAFADAVGTTLIGEGVETWE
                     EVESLAQLGVRYAQGFLLGRPSFERTEPDPAMRKKLASLYGPWRKEHGMELGILLRDL
                     REPCETFSSEGRTCEELDRTFRKNPEIDHLVLVENGRVSGLVTRQHFYFITGGPVGYS
                     LYQKRPAHSVAKKNPLKVQEGISVTTLADLAMKRKPEDLYDPVVVTDTEDRVLGTVTM
                     RKLIMRSKELETERALDCNPLTGLPGNRAIERWISSALAQPSFTLIYADLDHFKEYND
                     RYGFLRGDDLIRFTAGLFRDFLPKIGPEACLGHVGGDDFVFISPGRVREEALAALCEA
                     FDQGKERFFDPEDLERGAYHALNRAGELQAIPLVTLSLAVIVESNLPENPHPGVLSSE
                     SAALKKAVKKQNGQTRKSGFLVDRRLCPVGGIIFQPPSGFSACFRQRNPQVEIPLN"
     gene            5279..>5607
                     /locus_tag="PLY83_04750"
     CDS             5279..>5607
                     /locus_tag="PLY83_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29375.1"
                     /translation="MARSNPSRFLKFLAFAGVSLATLCLALQPVHALDFSFGRKPLDP
                     SVQNALAQMELTPQNFFKWQSEKPTSLLLQIHRWPAEKQQMTAALALYIRSQNTNVSA
                     RDIWRQAA"
BASE COUNT         1062 a         1816 c         1624 g         1105 t
ORIGIN      
        1 ccccctttcc tggaggcggc ggacctgcca tccttttccc ggaatcgccc gaaaccgaga
       61 aagggaggtg cccgttttga agatctgcat cgccacggac gcctggactc cccaggtcaa
      121 cggagtcgtg agaaccctga cggaaacgga aagacgcctc cggaagatgg gacacgagat
      181 cttcctgctg accccggagc gcttcgccac ggtcccctgt cccacctacc gggaaatccg
      241 cctggcctgg agcctttggg acgtcgaccg cttcctgcgg gactttctcc ccgacgcggt
      301 ccacatcgcg acggaagggc ccatcgggtt ctccgtccgg gcctggtgcc tccgggaagg
      361 gttccccttt acgacttcgt tcacgacccg tttccctgaa tatgtccgtt cgcgcttcgg
      421 gctttccgcc gaactgctgt accaggggct gcgctggttc cacgccccct cctcggcggt
      481 gatggtggcc accgcctcgc ttcaggaaga gctacaggga cgcggttttg ccaatctcgt
      541 ccggtggtcg agaggggtgg acaccagcct gttttgcccc tcgcttccac gccatcccgt
      601 tcgaaaccgt ccggtcttcc tttacgtcgg ccgcgttgcc gttgagaaaa atctcccggc
      661 cttcctggac ctggatttgc ccgggacaaa gcgcgttgtg ggctcgggcc tcgacctgga
      721 ggctctccgc aggacctacc cggcagtgga gttttccggc accctggaag ggcttgatct
      781 tgcccaggca tacgccgagg cggacgtttt cgtgtttccc agcaaaaccg acactttcgg
      841 gatcgtcatg ctggaggctc tcgcctgcgg aacgcctgtc gccgcctatc ccatgacagg
      901 accgctggac atcctccgcc agaacgtgac cggagtgatg gacttcgatc tcaggaaagc
      961 ctgcctggaa gccctttccc ttgaccgggc cgcctgccgg caggacgccc ttctatggtc
     1021 ctgggaccgg tgcacgcggc aattcctgga aaacctccgg ccgcgttcct tttcgaagct
     1081 cgttcacgat cctgttggag ggatgtaaaa cccctgtaag ggcgatcgga ttacactcgc
     1141 ggcgaacctg tggggacttt catcctttca tccgggagga atccgattga aacccgtcct
     1201 tttggcctat tccggccccg ggcagggcca caagatggca gccatggccc tgcgggaagc
     1261 ctttctcgcc gaaaaggcgc ccgtcgtctg cctggacacc ctcaccacgg cgatgccgtt
     1321 tttccggttc ctttacgcag ggctctacaa gggaatggcc cgccacgccc acacggcctg
     1381 cgagcaggtt taccggctca cggaccgggt ccgcgggaaa agtcccgtgg tccgggccat
     1441 cgactggtgg agccttcaga acacaacggt cttccaggcc ctggcgaaaa cccttgcacc
     1501 ggaaacggcc gtttgcaccc acttccttcc catggccctc ctgtccctca tgagggaacg
     1561 gggggaattc aacggacaaa tccatgttgc catcaccgac tacgatctcc acggattctg
     1621 ggtcgaccct ggcgtggacc gctactacgc cgcctcggtc caggtccgtg accggctggc
     1681 cgccaggggg atccccgatt cgcggattgc ggtcaccggc atcccggttc gggaaagctt
     1741 tacgaggctt gccctttcac cgcggatccc caggccgctt ccgcttcgaa ttctcttcct
     1801 ggcccactcc atcgcccgtg aaaccgccct gtcgatcatc gacagcctct cttcgatcgg
     1861 tccctccatc cggctcaccg tggtcgggga aagggaactc tcgaaaaacc ggcccaaagc
     1921 ttcgatctgc tggtttgaac ggatcgaaaa cctggcccct ctcatggcag acgccgacct
     1981 ccttgtcacc aagcccggcg ggctggtctg ctccgaagcc ctctcggcgg ggctgcccat
     2041 gctcttcatt tctccgatcc ccctgcagga aaccctgaac gcgcagtatc ttcaggggca
     2101 gggcgcgggg atcttctgcg aatccccggc ggccctggca ggagaggtcc gcgggatcct
     2161 ggccgacccc ggaatccttc ggaggatggg acacgcctgc cagcgcatgt ccgccccgga
     2221 cgccgcccgg gcaatcgccc gcagggtcct cggcaggcgc ccccaggtcc cggaatggga
     2281 agcccccgct gccttctgtc accctggagg aaaccgaccg tgctttccgt gaggaccgcc
     2341 ttcctttccg accttcattt ggggtcccga tggtgccggg cagcggagct gtccgccttc
     2401 ctcgatctcc tcgagtgcga gacccttttc ctcgtcggag acatcgtcga cggatggtcc
     2461 ctgaaaagaa ggttccgctg gccggatgcc catacggaag tcctggcgaa aatcctcgat
     2521 ttctccagga acagccgggt ggtctatgtg accggtaacc atgacgcgtt cctggacagg
     2581 cttgcgggcc ggaaactggg aaacattgaa atccgccttt gtgcgatcca tgagaccgtt
     2641 cgggggactc gcctggcggt tttccacggc gacgaagtgg accgggtgtc ctccgagcag
     2701 ccctggctgg cccatctcgg cgattttgcg tacacggcag ccctgtgggc aaaccgggga
     2761 gccaacgttc tcaggagggg ctttgggctt gaataccgct cctattccag gatcctcaag
     2821 cagaaggtca aggaagccgt caactacgtc agcgggttcg agaagaaact ggcggaagag
     2881 gcccgccgcc tgggcacaga cggagtcatt tgcggccaca tccacaagcc cgcctgggaa
     2941 gaaagccgag gcgaagtctt ttacggaaat tgcggcgact gggtcgaaag ctgctccgcc
     3001 ctggtggaga agcatgacgg ggagatggag gtcctggact gggagaccct gaagcaaacg
     3061 gcgggggtcc cagtggtgac ccaaaacgga gagatggtct accggttccc cctgccggaa
     3121 gcggcttcct cctgggcagg cgccttgctt tccatgaacg tgggtcccac ggatcttcgg
     3181 gacgtcctaa agacacgccg atgaccctcg atccggcttt cctcctggag caaatcctgt
     3241 cggagaaggc cgttcggatt gccttccagc ccatcgtgga cctttccgac gggtccgttt
     3301 tcggccacga ggccctggcc aggggaaagg gagccctgga aatgccggct gcccttttcc
     3361 cctgcgccag gaccctggga aagacctggg aacttgaaaa aacctgccgg gaaaacgcgc
     3421 tggcggcaat gggaaaggga aacagcccgc ttcagccctg ccgcctgttc ctcaacgcaa
     3481 gcccccgggt attcgccgat ccccgattcc cggaagttct cgcacgggaa atcgggagga
     3541 tacccgggat ttcccgtgac cagatcgtcg tggaactgac cgagcaggaa gggcttgaag
     3601 acgagtcgct cctgaagttc caggccaggc acctttccgg gcacgggttt tcggtcgccc
     3661 tggacgattt cggcgcgggg aactccggac tccggatcct gatggaatgc tgtccggcct
     3721 tcctcaagct cgaccgcggc atcatccgcg gcatcgaccg ttcagccctg aggatgcagc
     3781 ttgtgaagag tctcgttgct ttcgccgatg ccgttggaac aaccctcatc ggtgaaggcg
     3841 tcgaaacctg ggaagaggtg gaatccttgg cccagctcgg ggtccgctac gcccagggat
     3901 ttctcctggg ccgcccctcc ttcgaacgga ccgaaccgga tcctgcgatg cggaaaaaac
     3961 tggcctctct atacggcccg tggaggaaag agcacggcat ggagctcggg attctccttc
     4021 gggacctccg tgaaccttgc gaaaccttct ccagcgaggg ccggacctgc gaggaactcg
     4081 accggacgtt ccgcaagaac ccggagatcg accacctcgt cctggtcgag aacggcaggg
     4141 tttccggcct ggtcactcgc cagcatttct acttcatcac gggcggccct gtgggctatt
     4201 ctctctacca gaagaggccg gcccattccg tcgccaagaa gaacccgctg aaggttcagg
     4261 aagggatctc ggtcacgacc cttgccgacc tggcgatgaa aagaaagccg gaagaccttt
     4321 acgatcccgt ggttgtgacg gacaccgaag accgggtcct cggaaccgtc acgatgcgaa
     4381 agctcatcat gcggtcgaaa gaactcgaaa cggaaagggc cctggactgc aatcccctga
     4441 ccggccttcc ggggaaccgg gcgatcgaac ggtggatctc atccgcattg gcgcagccct
     4501 cttttacgct catttacgca gacctcgacc acttcaagga atacaacgac cggtacggct
     4561 ttctccgcgg cgacgacctg atccgcttca ccgcgggcct tttccgcgat ttcctgccga
     4621 agatcgggcc ggaggcctgt ctcgggcacg tcggcgggga tgatttcgtc ttcatttccc
     4681 cgggcagggt tcgtgaagag gccctggcgg ctctgtgcga agcttttgat caggggaaag
     4741 aacggttctt cgatcccgag gatctcgaac ggggagccta ccatgccctc aaccgggccg
     4801 gcgaactcca ggccatccct ctcgtgaccc tgagcctggc cgtgatcgtc gagtcaaacc
     4861 tcccggaaaa ccctcaccct ggagtcctct ccagtgagtc cgcagccctc aagaaagcgg
     4921 tcaagaagca aaacggccag acccggaaga gcggtttcct ggtggaccga agactgtgtc
     4981 ccgtcggcgg catcattttc caacccccct ccggtttttc cgcctgcttc cggcagcgga
     5041 acccccaggt tgaaatccct ttaaattaac tttcgtttct ttcaaaattc cagggattcc
     5101 cgaatctccc gcgatcgaca ctgccctccg cccactagta aactaaaatc tactaggggt
     5161 ttatatttaa cattggagta caatacttgc gccgtttttc tcattcggta taatgggcca
     5221 attcgcaaag ggagattgaa aaccgatgcg gaaaagcccc aggaagggag gaaacttcat
     5281 ggcgcgctcg aatccgagcc gatttctgaa gtttctggcc tttgccggag tttcgctggc
     5341 gactctctgc ttggccctgc aacctgtcca tgcacttgat ttcagctttg gccggaaacc
     5401 cttggatcct tcagttcaaa acgcgctggc acaaatggaa ctgaccccgc agaacttctt
     5461 caaatggcag tcggaaaaac ccacatccct ccttctgcaa atccaccgct ggcccgccga
     5521 aaagcagcaa atgaccgcag ccctggctct gtacatccgg agccagaata cgaacgtctc
     5581 tgcccgggac atttggcggc aggcggc
//
LOCUS       DAOOVN010000043         5587 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_8219_length_5587_cov_14.319234, whole genome shotgun sequence.
ACCESSION   DAOOVN010000043 DAOOVN010000000
VERSION     DAOOVN010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5587
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8219_length_5587_cov_14.319234"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="PLY83_04755"
     CDS             complement(<1..1064)
                     /locus_tag="PLY83_04755"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOV29376.1"
                     /translation="MSLSVLLLALHNLFSFLIRLAALAVLPVRHSPPTAMAWLLIVFF
                     WPLPGAALYLLMGSTRLPRARKIRHEKAMRLLRRIQDKPRRVDPTPFPPVLPDHAERF
                     ARLGQKLGKWPVVAGNRMEFIHSAAGLFEKLAEDIDGAEHHVDLLYYIFAADRVTRPL
                     LESLKRAAARKVECRLLLDALGSKAFLKKHAHSLQDAGVQVVEALPLGRLFRRNRVTA
                     RYDLRNHRKLAVIDGRVGYMGSHNAIDPTYGGKAKGIAWHDLTLRLEGPIVSQLEGVF
                     LEDWFVETEELPPGRRNGIFDSPKGDFLLQAVPSGPAYATENFQRLVLAALFEARERV
                     IITTPYLIPDDGLLQAMEVAV"
     gene            complement(1061..1816)
                     /locus_tag="PLY83_04760"
     CDS             complement(1061..1816)
                     /locus_tag="PLY83_04760"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOV29377.1"
                     /translation="MRVVLYNIRYGTGTGWDYHLPLPFSGLFRRTEPSLRRVSSFIDE
                     IQPDIVGLVEVDGGSYRHGGESQARCLAEDLKLDHFFSCKYGRESIFRKLPILSSQGN
                     AILSRVPAKRTSKIDLERGMKKTLLAAEFEEFVFLLVHLPLGLGARKAQLDHLTEIVR
                     ECRKPVLLGGDFNLLHGSAELSGFLSRTGLRDADPLERKTYPSRLPRFRLDFLLIGPN
                     VIVRRFDVPDVRFSDHLPLICDFEVLPEEREGQ"
     gene            complement(2003..2959)
                     /gene="arcC"
                     /locus_tag="PLY83_04765"
     CDS             complement(2003..2959)
                     /gene="arcC"
                     /locus_tag="PLY83_04765"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOV29378.1"
                     /translation="MSATKVVIALGGNALQEAGTPPTAEAQLEVVKKTAGYLAEISSR
                     GYEMAIVHGNGPQVGRIVLGQEIASRENDQTPAMPFDVCGAMSQGYIGYHIQQALRDA
                     LRNRNRNVPVVTIATQVVVDPNDPAFKNPTKPIGPFYTEEEARKLEIEKGYTMKEDAG
                     RGWRRVVPSPEPKKIVELSAIKRLWDTTIVITAGGGGIPVIENMDGSLTGVAAVIDKD
                     LAAERLAEDIEADILLILTEVDKVCLNFKKPDQKVLDHMTVPEAIRYMEEGHFAPGSM
                     LPKVLAAIKFARTFPGRKAIITSLYKAVEALEGKEGTVVTMA"
     gene            complement(3113..3835)
                     /locus_tag="PLY83_04770"
     CDS             complement(3113..3835)
                     /locus_tag="PLY83_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807155.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOV29379.1"
                     /translation="MRSRLTVAAAVLGALVGLGLALAGFFVGEALKEARSAERFVTVR
                     GFAERNVQADLVIWPITFRDTGNDLAELQKVVDSHKNEVRAFLAAAGFRPEEISEMPP
                     QVTDLKAQAYGDGQKRDYRYIATATITLRTSDVKAAKKAMEDSGKLVLRGIVLAEQDY
                     ARSTEFLFTGLNEIKPAMIAEATKNAREAAEQFARDSGARVGSIRRASQGLFTIEDRD
                     KGSPDHKKVRVVTTVEYFLSSD"
     gene            complement(3863..4330)
                     /locus_tag="PLY83_04775"
     CDS             complement(3863..4330)
                     /locus_tag="PLY83_04775"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOV29380.1"
                     /translation="MTGEERERQLRDCGFPFELLENRVRIRSASEGAAYWGIELGATA
                     PALVLRAQGGFWLLLVSGAIRKVDFAALGRETGEEGMRLATSAEILERFGLKPGEVPL
                     FGLGLPTLVDRKLLAYEFVYGGSGDPFRTLKIAPEALLVLNEESRLIDVPAMP"
     gene            4416..>5587
                     /locus_tag="PLY83_04780"
     CDS             4416..>5587
                     /locus_tag="PLY83_04780"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HOV29381.1"
                     /translation="MSQLNFFRSFLRGREPQPRAALEFLRYIGPGILVTVGFIDPGNW
                     AANVAAGSGFGYSLLWIVTLSTVMLVLLQHNAAHLGIATGLCLSEAATEHLPAWLSRT
                     LQVSALGAAASTALAEILGAAIALQILFRLPLPVGGALAAAFSLWALLTNSYRKLEKW
                     IIAFVSLIGFSFLFELSLVKVPWDQALKGWVVPTVPEGSMLIVMSVLGAVVMPHNLFL
                     HSEVIQSREWHLLDESAIRKQLRYEWMDTIFSMGIGWAINSAMVLLAAATFFSAGIRV
                     TELEQAEKLLRPLLGNAAALVFSLALLFSGLSSSVTAGMAGGSIMAGIFREAYNIRDR
                     HSRFGVFLTLGTGCLVLFFVKDPFSGLLFSQMLLSIQLPVTIVCQILLTSSRKVMGR"
BASE COUNT         1054 a         1773 c         1592 g         1168 t
ORIGIN      
        1 accgccacct ccatggcctg aagcagcccg tcatcgggga tcaggtaagg ggtggtgatg
       61 atcacccgtt ccctggcctc gaaaagagcc gccaggacca ggcgctgaaa attctccgtc
      121 gcgtaagccg gaccgctcgg cacggcctgg agcagaaaat cccccttcgg ggaatcgaaa
      181 atcccgtttc tccgcccggg agggagctct tcggtttcaa caaaccagtc ttcgaggaaa
      241 accccctcca gctgagatac gatggggcct tccaaccgca gggtcaggtc gtgccaggca
      301 atccccttgg ccttcccccc gtaggttgga tcgatggcgt tgtgagatcc catgtacccg
      361 acccgcccgt cgattaccgc caactttcga tggtttcgca ggtcgtagcg cgcggtcacc
      421 cggttgcggc gaaacagcct ccccaggggg agagcctcga cgacctgcac gccggcatcc
      481 tgcagggaat gcgcgtgttt cttcagaaag gctttcgatc ccagcgcgtc caggagaagc
      541 cgacactcca ccttcctggc tgccgcccgc ttcagggatt ccaggagggg gcgggtcacg
      601 cgatcagcgg cgaaaatgta ataaagcagg tcaacgtggt gctcagcgcc gtcgatgtct
      661 tccgccagct tctcgaaaag acccgcggca gaatggatga actccatccg gtttcccgcg
      721 acgactggcc attttccaag cttctgcccc aggcgggcga accgctccgc gtggtccggc
      781 agcacaggag gaaaaggagt cgggtcaacc ctgcgaggtt tatcctggat gcggcgaagg
      841 agtctcatcg ctttttcgtg acggatcttc ctcgctcgcg ggagacgggt ggaccccatg
      901 aggagataca gggccgctcc cgggagcggc cagaaaaaaa cgatgagcag ccacgccatg
      961 gccgtaggag ggctgtggcg gacaggaagg acggcgaggg cggcgagccg gatcaagaac
     1021 gaaaaaaggt tgtgaagagc gagcaggagg acgctgaggc tcattgccct tctctttcct
     1081 ccgggaggac ttcaaagtcg cagatgagcg gaaggtgatc cgaaaaccgc acgtccggaa
     1141 cgtcaaaacg gcgaacgatc acgttggggc caatgaggag gaaatccagg cgaaaccggg
     1201 gcagacggct cgggtaggtc ttccgctcca gcggatcggc atcccttagg ccggtcctgg
     1261 ataaaaatcc gctcagttcg gcgctgccgt gcagcaggtt gaagtcgccc cccagcagga
     1321 ccggctttct gcattccctg acgatctcgg tcaagtggtc gagctgggct ttcctggcac
     1381 ccaggccgag gggcaggtga acgagaagga agacgaattc ctcgaattcg gcagccagga
     1441 gggttttttt cattcccctt tccaggtcaa tcttgctcgt ccgtttcgct ggaaccctgg
     1501 aaaggatggc gttcccttgc gaactgagga tcggcaactt cctgaagatc gattccctcc
     1561 cgtatttgca ggaaaagaaa tggtccagct tgagatcctc cgccaggcat ctcgcctgcg
     1621 attccccccc gtgccggtag gacccgccgt ccacctccac cagcccgacg atgtcgggct
     1681 ggatttcgtc gataaaggaa gaaacccgcc tcagggacgg ttccgttctc ctgaaaaggc
     1741 cggaaaaagg caggggtaaa tggtaatccc accccgttcc ggttccatag cgaatgttgt
     1801 aaaggacaac cctcaacggc actcctcccg tgggccgaac tggccttcta tcctggatcc
     1861 gacaggatcc ggggttcacc cggatgcccc cggacccgtg agaaacatgg ggttaacggt
     1921 ttttattcta ccgtaaagaa aaacgaaagc ccggcgtccc cttctgcgcg agagaacgcc
     1981 gggctttcgg ttttccagga ttctaggcca tcgtgacgac ggttccttct ttcccttcca
     2041 gggcttcgac ggccttgtag agagaggtga tgatcgcctt gcggccaggg aaagtcctgg
     2101 cgaacttgat tgccgccagc accttcggca acatgctccc gggagcgaaa tgaccttcct
     2161 ccatgtaccg aatggcttct ggaacggtca tgtgatcgag gaccttctgg tccggtttct
     2221 tgaaattcag gcagaccttg tcgacttccg tcaggatcag gaggatatcc gcctcgatgt
     2281 cctcggccag gcgctcggcg gcaaggtcct tgtcgatgac cgccgcgact cccgtaagcg
     2341 aaccgtccat gttctcgatg accgggatcc cgcccccgcc ggcggtgatg acgatcgtcg
     2401 tgtcccaaag ccgcttgatg gcagagagct cgacaatctt cttcggttcg ggggaaggga
     2461 caaccctcct ccaccctctt ccggcatcct ccttcatcgt gtagcccttc tcgatctcca
     2521 gcttgcgggc ttcctcctcg gtatagaagg ggccgatggg tttcgttggg ttcttgaatg
     2581 ccgggtcgtt cgggtccacg acgacctggg tggcgatcgt cacgaccgga acattgcggt
     2641 tgcggttccg aagagcgtcc cgaagcgcct gctggatatg gtagccaatg tatccctggc
     2701 tcatcgcccc gcacacgtca aacggcatcg caggggtctg atcgttttcc cgggaagcga
     2761 tctcctgccc gaggacgatc cgccccacct gcgggccgtt cccgtgaacg atggccatct
     2821 cgtatccccg gctgctgatt tcggccaggt accccgcggt cttcttcacc acttccagct
     2881 gcgcttccgc cgtcggagga gttccggcct cctgaagggc gttgcctccc aatgcgatga
     2941 cgacctttgt tgcgctcatg gtgttttctc tcctcttttg aaagatctct tcttcggaga
     3001 gaattgtaaa ccataacacc actacagttc aatatctctg gtaaccccct ggcgagaatt
     3061 cttccgtccc ccgaggggtt gaatttggct gaagcattgc gatcatttgc tttcaatcac
     3121 ttgaaagaaa atattccacc gtggttacaa cccggacttt cttgtgatct ggtgaacctt
     3181 tatcccggtc ttcgatcgta aagagtccct gggaagctct ccggatgctg cccacgcgag
     3241 cgccggaatc ccgggcgaac tgttccgcgg cttcccgggc attctttgtt gcctccgcga
     3301 tcatcgcggg cttgatttcg ttaagccccg tgaagaggaa ctccgtcgac ctggcataat
     3361 cctgctccgc caggacgatc ccgcgaagga ccagtttccc ggagtcttcc atcgcctttt
     3421 tggccgcctt gacatccgag gtccgaaggg tgatcgtcgc cgtcgcgatg tagcggtaat
     3481 ctctcttctg cccgtcccca taggcctggg ccttcaggtc agtgacctgc ggaggcatct
     3541 cggaaatctc ctcgggcctg aaacccgccg cggccagaaa agcccggacc tcgttcttgt
     3601 gggaatccac gactttctgc agttctgcaa ggtcgtttcc cgtatcccgg aaggtgatgg
     3661 gccagatgac aagatccgct tggacattcc gctcggcaaa tcccctgacc gtaacgaacc
     3721 gctcggcaga acgggcctcc ttcagggctt ctccgacaaa gaaacccgca agggccagcc
     3781 cgagtcccac caaggctccc aataccgccg ccgccaccgt caaacggctt cgcatgcgcg
     3841 ttcccccttt ttcgtttctt cgtcaaggca tggcaggcac atcgatcagc ctgctttcct
     3901 cgttcagcac cagcagggct tccggggcaa tcttcagggt ccggaaggga tccccggaac
     3961 ccccgtaaac gaattcgtag gcaagcagct tccggtcgac cagcgtcggg agcccgaggc
     4021 cgaagagagg aacttccccg ggcttcagcc cgaaacgttc caggatttcc gcggaagtag
     4081 ccagcctcat cccttcttct cccgtttcgc gtcccagtgc cgcgaaatcc accttccgga
     4141 tcgcccctga gacgagcaga agccaaaaac cgccttgcgc ccgaaggaca agggcgggag
     4201 ccgtggctcc cagctcgatc ccccagtagg ccgcaccctc ggaagcgctc cggatccgga
     4261 ccctgttttc aaggagttcg aaggggaagc cgcaatcccg aagttgcctc tctcgttcct
     4321 cccccgtcaa gcgatcgcct ccccaggctg gcaaaaagcg tggccagccc ctaagatgga
     4381 accggttcgc acccctcaat tcgaggtgga cacggatgtc acagttgaat ttctttcgtt
     4441 cattccttag aggccgggaa ccccagccgc gggcggccct cgaattcctc cgctacattg
     4501 ggccggggat cctcgtgacc gtcggcttca tcgatcccgg aaactgggcc gcaaacgtgg
     4561 ccgccggatc aggcttcggc tactcccttc tgtggatcgt aacgctgtcc accgtcatgc
     4621 tggtcctgct gcagcataac gcggcccacc tggggatcgc cacgggactc tgcctttccg
     4681 aagcggcgac ggaacacctc ccggcctggc ttagcaggac gctccaggtt agcgccctcg
     4741 gggccgctgc ctccaccgcg ctggcggaaa tccttggggc cgccatcgcc ctgcagatcc
     4801 ttttccggct gcccttgccg gtgggaggag cactcgccgc ggctttttct ctctgggccc
     4861 tcctgaccaa ctcctaccga aaactggaaa aatggatcat tgcgttcgtt tcgctcatcg
     4921 gtttttcttt cctgttcgag ctgagcctgg taaaagtccc ctgggaccag gcgttgaagg
     4981 ggtgggtcgt cccgacggtt ccggaggggt cgatgctgat cgtcatgagc gtgctcgggg
     5041 ccgtcgtcat gccccacaac ctctttctcc attccgaggt gatccagagc cgtgagtggc
     5101 acctcctgga cgaatccgcc atacggaagc agctgcgcta cgaatggatg gatacgatct
     5161 tttccatggg aatcggctgg gcgatcaaca gcgcgatggt tcttctcgcc gccgcaacat
     5221 ttttctcggc agggatccgg gtcaccgagc tcgagcaggc ggagaaactc ctccgtcccc
     5281 tcctgggaaa cgcggcggcc ctggttttct ccctggccct cctgttttcc ggcctgtcct
     5341 ccagcgtaac cgcaggaatg gcgggcggtt cgatcatggc gggaattttc cgggaagcct
     5401 acaacatccg ggaccggcac agccgcttcg gggtttttct cacccttggg acgggttgcc
     5461 tggtcctttt tttcgtcaag gaccccttca gcgggctgct cttctcccag atgctcctca
     5521 gcatccagct tcccgtcacg atcgtctgcc agatcctgct gacttcctcc aggaaagtca
     5581 tgggacg
//
LOCUS       DAOOVN010000044         5365 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_8616_length_5365_cov_13.240678, whole genome shotgun sequence.
ACCESSION   DAOOVN010000044 DAOOVN010000000
VERSION     DAOOVN010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5365
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8616_length_5365_cov_13.240678"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..2176)
                     /locus_tag="PLY83_04785"
     CDS             complement(71..2176)
                     /locus_tag="PLY83_04785"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOV29382.1"
                     /translation="MEHRIFLEGLCCAQCAADMEQNLRQMEGVRSVRFNLATGILFVE
                     TADVEEVIAHVKRLLPEVEVRLAKPGERLAEGPAPFPGESWKPLVLPGVLFVAGWILS
                     LAPGGMLDPAAAVVHAGTWLFAGLPVFRSAMAGITMHRPFDESILMTIATTGALALGA
                     FPEAAGVMIFYRVGMALEDLAVARSRRSIRGLLEARPASAWRRSASGFFEEVPPEEVA
                     PGEILRVLAGEMIPVDGRVEEGEALVDLSALTGEPFPSGAGPGAHVRAGGILVAGAIV
                     YRATAPFRESSIARILDLVENAASRKSPAERFITRFSRIYTPVVVLGAASIAVLPPLL
                     FGGAFPVWAYRALVLLVISCPCALMIGVPLGYFGGIGAASRAGVLVKGASVLDALADV
                     KTVLFDKTGTLTHGVLKVVSSETAPGFDEAENRRLVASAEAWSVHPAAKALVGLVSPE
                     SLFLDVKDVRESLGRGMSALVGGRRVLAGTAAWLEAEGVPVPPEPPFPGMTVNLAVEG
                     NYAGRFFLSDAVREEAAGAVRALRRAGVKRIFMLSGDREENARQVAVELGLDDYRAGL
                     LPERKVEELEKVLAMDPAERGRVAFVGDGLNDAPSLARADVGIALGGASADAALETAD
                     AILSDDRLSRVSKALTIAHQTRRIVRQNIALTLGLKGLVLALGVFGEATLWEAVFADV
                     GVTLLAVINARRLLKVRLK"
     gene            complement(2204..2500)
                     /locus_tag="PLY83_04790"
     CDS             complement(2204..2500)
                     /locus_tag="PLY83_04790"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HOV29383.1"
                     /translation="MPGREIRVVFRGLPSEAETRGTELWAMLSEGSTVSDLLEKLASA
                     STGKASLVLDPARKTLRPEFLMTLNGRFLEKSRFASTALKDGDTVEVFPVPSGG"
     gene            complement(2490..4349)
                     /locus_tag="PLY83_04795"
     CDS             complement(2490..4349)
                     /locus_tag="PLY83_04795"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOV29384.1"
                     /translation="MGERMNGYLGRLANVNLSTGCVREEVLDELTARKWVGGAGLGAK
                     ILLEEIPPGVEWDAPENRLVFTAGPLAGTMMFGSGTFNVATKGPMTNLAVISQANGYF
                     GAYLRLSGFDGLVLRGRSAEWVTLWVHDGRVEIRPAAALLGLDAWETEDALRGELGVG
                     ERDVSIFAIGPAGENGVRYAAIVGDRGHVASKNGVGAVMGSKRVKAVVASRGTFEVPV
                     FDPQALRRANLECFEAAKAFGTTWKWGTGGGFSNLAKSGALPVKNYTTSLFPEHESMN
                     GQTLRTKFRVRSMPCFRCAISHVKEVTVTEGPYAGFTGEEPEYEQLAAWGPQIGNTDL
                     GATVLLTREVDRLGMDCNEASWAIGWAMECFEKGVFSLSETEGLDLSWGNVEAVRVLL
                     GRISRREGFLGALLAEGVMRASRKVKGPAEQWAIYTLKGVTPRGHDHRGGGRWPELFD
                     TCVSDTGTIQSSWSGIHPQFVDQPPLEDVFDPAEVSRVVGDFAGYRTFDDCLGICRLA
                     APHPKPLLACFNAVTGWNWTLADAREAGLRVITALRLFNLRHGLDPENERPSARYGSI
                     PVDGPAQGRDIQAKWDEMRADYYRRMGFDPQTGIPTAETLKRLGLEAYAPHAG"
     gene            complement(4373..5131)
                     /locus_tag="PLY83_04800"
     CDS             complement(4373..5131)
                     /locus_tag="PLY83_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HOV29385.1"
                     /translation="MRWRMGWIGIALLMIKLAVPASAGPLEEWAAAIDARDRGMASAA
                     SLRAELAGQAGEGSPVERWRRLLRGDESPEARAADAVALVDALFPAGDPARWEEVKGF
                     WRAAEVPLPLAALDAVFYGAKALLSMQDGRAPWLARVLVEALAASDRARTVAFRAAPE
                     EVREVLEELSLKAPPPPTGGWPSGKPLGRLPFAHGVSGWITETRATMEGMTFLNAAGV
                     PVGGTGVYAWDRERGRIYRVLDSEPRRIWLFYDD"
BASE COUNT         1001 a         1888 c         1585 g          891 t
ORIGIN      
        1 gttcgtcctc cctgctagac aaaacgcgaa gacccccgct ttcctgacgg gggtcttcga
       61 aaaaggttct ttacttcagg cgaaccttta gcagcctccg ggcgttgatg accgcgagga
      121 gggtcactcc gacatcggcg aaaacagcct cccagagagt cgcttccccg aaaaccccca
      181 gggctagaac caggcccttg aggccgagcg tgagggcgat gttctggcgg acaatccgcc
      241 gggtttggtg agcgattgtc aaggctttcg acaccctcga aagccgatcg tcggacaaaa
      301 tggcgtctgc cgtctcaagg gcggcatccg cggaagcccc tcccagggcg atgccgacgt
      361 ccgcacgcgc cagggaaggg gcgtcgttca ggccgtcccc gacaaaggcc acccggcccc
      421 gttcggccgg atccatcgcc aggacctttt caagctcctc gactttccgt tccggcaaaa
      481 gcccagcccg gtagtcgtcc agcccgagtt ccacggccac ctggcgggca ttttcctccc
      541 ggtcccccga gagcatgaag atccgcttca cgccggctcg cctcagcgca cggacagctc
      601 cggccgcctc ttccctgacg gcgtccgaca ggaagaacct tcccgcgtaa ttcccctcca
      661 cagccaggtt gacggtcatt cccgggaagg gaggttcagg aggaacgggc accccctccg
      721 cctccagcca ggccgcggtt ccggccagga cccgcctgcc tccgaccagg gccgacatgc
      781 cccgccccag ggattcccgg acgtccttca catccagaaa gagggattcc ggagaaacga
      841 gtccaacgag agccttggcg gccgggtgaa cggaccaggc ctccgccgat gccacgaggc
      901 gccggttttc cgcctcgtcg aagcccggag ccgtttcgga ggacaccact ttcagcacac
      961 catgcgtcaa ggttcccgtc ttgtcgaaca aaacggtctt cacgtctgcg agggcatcga
     1021 ggaccgaggc cccttttacg agcaccccgg cccgcgatgc ggccccgatg ccgccgaaat
     1081 atcccagggg aacaccgatc atgagcgcac agggacacga gatcacgagc aggacaaggg
     1141 cccggtaggc ccacaccggg aaggccccgc caaacaggag gggcggcagg accgcgattg
     1201 aggccgcccc gagcacgacg acaggtgtgt agatccgtga aaaacgggtg atgaagcgtt
     1261 ccgcggggga tttcctcgag gccgcgttct cgacgagatc gagaatgcgc gcgatggagg
     1321 attccctgaa gggcgccgtc gcccggtaaa cgatcgctcc agcgacgagg atgcctcctg
     1381 cccggacgtg cgcacccggc cccgcacccg acgggaaggg ttctcccgtc agggcggaaa
     1441 ggtcgacaag ggcttccccc tcctccaccc tcccgtcaac ggggatcatt tcccccgcaa
     1501 ggacccggag gatctccccc ggagcgacct cttcgggcgg aacctcctcg aaaaagcccg
     1561 acgccgagcg gcgccaggcc gaggcggggc gagcctccag aaggccccgg atggaacgtc
     1621 tcgaacgggc cacggccagg tcctccagcg ccatgcccac ccggtagaag atcatgacac
     1681 cggcagcctc ggggaacgcc ccgagggcaa gggcccctgt cgtggcgatc gtcatgagga
     1741 tcgactcgtc gaaggggcgg tgcatggtga ttcccgccat tgcggatcga aacacgggta
     1801 ggccggcaaa cagccaggtt cccgcgtgga ccacggcggc cgcagggtcc aacatgcccc
     1861 ctggcgcaag ggaaaggatc catcccgcga cgaagaggac cccgggcagg accagtggtt
     1921 tccaactttc cccggggaat ggagcgggac cttccgccag tcgctcgcca ggcttggcaa
     1981 gacggacctc cacctcgggc agcaggcgct tgacgtgcgc gatgacctct tcgacatccg
     2041 cggtttccac gaagaggatg cccgtggcca ggttgaagcg cacggagcgg actccctcca
     2101 tttgcctgag gttctgctcc atgtccgccg cgcactgggc gcagcacaaa ccttccagga
     2161 aaatccggtg ttccatgcct tatcagtccc gatagttcaa aagctagccc cccgatggaa
     2221 ccgggaatac ttccacggta tccccgtcct tcagggcggt ggaggcaaag cgcgactttt
     2281 ccaggaacct tccgttcagg gtcatcaaga attccggccg cagggttttc cttgccgggt
     2341 ccaggacaag gctggctttc ccggttgaag cagaggccag tttttccagg aggtcactga
     2401 cggtgcttcc ctcggaaagc atcgcccaaa gttcggtacc ccgggtttcc gcctccgagg
     2461 gcagtccacg gaacaccacc cggatttccc tacccggcat ggggcgcata cgcctccagg
     2521 ccaagccttt tcagtgtctc ggccgtgggg atacccgtct gtgggtcaaa gcccatccgc
     2581 cggtaatagt cggccctcat ctcgtcccac tttgcctgga tgtcccgccc ctgggcagga
     2641 ccgtccacgg ggatggatcc gtaccgggcc gaaggccgct cgttttccgg gtcgagaccg
     2701 tgccgcaggt tgaaaagccg cagggccgta atgacccgaa ggccggcctc cctggcatcc
     2761 gccagggtcc aattccagcc ggtgaccgcg ttgaagcagg ccaggagggg tttggggtgc
     2821 ggggccgcca ggcggcagat cccgaggcag tcgtcgaagg tccggtaccc ggcgaaatct
     2881 cccacgactc tcgacacctc ggcagggtcg aacacgtcct ccaggggagg ctggtccacg
     2941 aactgcgggt gaattcccga ccaggaggac tggatcgttc ccgtgtccga cacgcaggta
     3001 tcgaacagct cgggccagcg ccctccgccg cggtggtcgt gccctcgggg agtgaccccc
     3061 ttcaaggtgt agatggccca ctgctcggca gggcctttga ccttccggga agccctcatg
     3121 accccctcgg ccagaagggc gcccaggaat ccctcccggc gggagatgcg gcccaaaaga
     3181 acgcggactg cctccacgtt gccccaggac aaatcgagcc cctccgtttc cgagagggag
     3241 aacactccct tctcgaaaca ttccatggcc cagccgatgg cccaggaagc ttcgttgcag
     3301 tccatcccga gccgatccac ttcccgggtc agcaggaccg tggctccgag gtccgtgttc
     3361 ccgatctggg gtccccaggc ggccagctgt tcgtattccg gctcctcccc ggtaaagccg
     3421 gcatagggcc cctcggtgac ggtcacttcc ttcacgtggg agatcgcgca ccgaaaacaa
     3481 ggcatcgaac ggacccggaa tttcgtccgc agggtctgcc cgttcatcga ctcgtgctcc
     3541 gggaacagag acgtggtgta attcttcacg ggaagagcgc cgctcttggc aaggttcgag
     3601 aagcccccgc cggttcccca cttccacgtg gtgccgaaag cctttgcggc ctcgaagcat
     3661 tccaggttgg cccgcctcag ggcctggggg tcgaaaaccg gcacctcgaa agtgccgcgg
     3721 cttgcgacaa ccgccttgac ccgcttggag cccatgacgg ctcccacgcc gttcttggag
     3781 gccacgtgtc cgcggtcacc cacgatcgcg gcgtatcgga ccccgttttc gccggccggg
     3841 ccaatggcga aaatgctcac gtcccgttcg cccaccccca gctccccgcg gagcgcgtct
     3901 tcggtctccc aggcatccag gcccaaaagc gccgcggccg gccggatttc aaccctgccg
     3961 tcgtgaaccc agagggtcac ccactccgct gaccttcccc ggagcaccag cccgtcgaaa
     4021 ccgctcagcc tcaggtaggc gccgaaatag ccgttggcct gggaaatgac ggccaggttt
     4081 gtcatggggc ccttcgtggc gacgttgaac gtgcccgagc cgaacatcat cgtccctgcc
     4141 agggggccgg cagtgaagac caggcggttt tccggggcat cccactcgac cccgggcggg
     4201 atttcctcca gcaggatctt cgcgcccaga ccggccccac cgacccattt ccgcgccgtc
     4261 aactcgtcaa gaacctcttc cctcacgcag ccggtagaaa ggttcacgtt cgccaggcgg
     4321 cccaggtagc cgttcatccg ttcacccatt cttctttctg cccccctcgc tctcagtcgt
     4381 cataaaaaag ccagatcctg cgcggctcac tgtccaggac ccggtagatc ctccctcttt
     4441 ctcgatccca ggcatagacg cccgttcccc ccaccggtac ccctgccgcg ttcaggaacg
     4501 tcatgccctc catcgtcgcc cgcgtttcgg tgatccagcc cgaaacccca tgggcgaagg
     4561 gaagccttcc cagcggtttt cccgagggcc accctccggt gggagggggc ggcgccttca
     4621 aagacagctc ctccagcact tcgcggactt cttctggcgc cgcccggaaa gccaccgtcc
     4681 gtgcccggtc cgaagccgcg agggcttcta ccagaacgcg agccagccag ggggcccggc
     4741 catcctgcat tgaaagcagg gccttcgccc cgtagaaaac ggcatccagg gccgccagcg
     4801 gcagggggac ctccgcggcc ctccagaacc ccttcacctc ttcccaccgt gccgggtccc
     4861 cggccgggaa aagggcatcc accagcgcca ccgcatcggc agccctggct tccggggact
     4921 cgtcgccccg gaggagccgt cgccagcgtt cgaccgggga gccctccccg gcttggccgg
     4981 ccagctctgc ccgcagcgaa gctgcactcg ccatcccccg gtcccgggcg tcgatcgccg
     5041 ccgcccactc ctccaaaggc cctgccgagg cagggaccgc cagtttgatc atcagcaggg
     5101 caatcccgat ccatcccatt ctccaccgca tcggacatcc ctcccttccc gcttcattgt
     5161 aaggccaaat ttccctaccg gaacgacact gaagcgccag accgaatgct cttccaatca
     5221 ctaccaaata agattgacat aggaatgctt cgatgaaaaa atacagcaag ggggttcttt
     5281 ttatatattt tcattctcat ttataccgtt ggaggtgatc ctgttgggac tcacaaaagg
     5341 ggaaaggaag tcgctgatct tcatg
//
LOCUS       DAOOVN010000045         5363 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_8620_length_5363_cov_14.263376, whole genome shotgun sequence.
ACCESSION   DAOOVN010000045 DAOOVN010000000
VERSION     DAOOVN010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8620_length_5363_cov_14.263376"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /gene="rlmD"
                     /locus_tag="PLY83_04805"
     CDS             complement(<1..1109)
                     /gene="rlmD"
                     /locus_tag="PLY83_04805"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOV29386.1"
                     /translation="MRRVPSQQLHLTIEKIDSQGEGLARNGQGKIVFIPGALPGETVL
                     AEVITEKRDYCTARLVNVLKPHPERVVPACPLHGKCGGCQIQHASYPFQLHLKLQMIT
                     DAFSRTGRQVPLEEFEAVRPSPLVWGYRNKISLPVGGGDGLLEIGYFRKKTHRIVPVE
                     HCPVAMAGLNGLLSEIRRGLGQTGLMPYNENTGKGTLRHVVLRGGQETGELLVVLVVR
                     EFPVSQVVDSLIALARRIRKRYPSLAGFVLNGNPSGGNAIFGPITRALSGRTFFFERF
                     GDMTFKFEATSFSQVNTSQAARLYETASGEACQAGTENVLELYAGVGTLTCFLAKRAS
                     KVLAVEEWKPSVDCLRDNAVKNGLSNVKIVEAAAER"
     gene            complement(1183..1737)
                     /locus_tag="PLY83_04810"
     CDS             complement(1183..1737)
                     /locus_tag="PLY83_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOV29387.1"
                     /translation="MSERFEIRFAGSGGQGVILAAVVTGEAAALYENVYAVQSQAYGP
                     EARGGASKSEVVVSHDVIDYPKATRPDLQVILTQKACEEYAHDTKKGGTIILDDFYVS
                     DPPQVDADIYMLPIVRTAKEKLGKEMVTNMVALGATARILELANVIRPDSVKKAILAR
                     VPKGTEKLNEQAFEEGYSMMKEKK"
     gene            complement(1743..2558)
                     /locus_tag="PLY83_04815"
     CDS             complement(1743..2558)
                     /locus_tag="PLY83_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOV29388.1"
                     /translation="MPREEIKPWLRTRFFPHIWCPGCGHGIILHAILRSLVDLGKTRE
                     ETVIASGIGCSSRMPGYINANTVHTTHGRSLAFATGIKLAKPELTVIDVMGDGDCTAI
                     GGNHFIHACRRNIDITAIVMNNNIYGMTGGQASPTTPVGARATTAPYGAIDTPFDICR
                     LAEGAGASYVARATIAQPALCEQYIKKAIQKKGFSVVEIITHCHTQFGRKNKRPNPVD
                     NLIFFKENSIPKAKAKEMTEEEKKGKIVIGEFVDREGLEYTEAYLKLIERVRG"
     gene            complement(2561..3697)
                     /locus_tag="PLY83_04820"
     CDS             complement(2561..3697)
                     /locus_tag="PLY83_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOV29389.1"
                     /translation="MPKKLAFWQGNMAIAMGGIAAGCRFFGGYPITPSSEIAEVMAEE
                     LPKINGRFIQMEDEIGGIAATIGASIAGYKAMTATSGPGFSLKQENLGLAYMAEIPIV
                     VIDVMRGGPSTGLPTRVSQQDVMQARWGTHGDHGTIAYAPSSVQECFELTIDAFNMAE
                     RFRQPVLVMSDEVIGHMREKVVIPEPGSYPIVDRKRPKVSPEEFVPYLADPADDVPAM
                     ASFGDGYRWHVTGLTHNDYGFPTNEPEEIGKKHLRLVRKVERFRDEIVKYDLEQTEGA
                     EILVVSYGCVARSAVRAVREARAKGIRVGHFRPITLWPFPDKELIQAAGQAKKVIVPE
                     LNCGQMISEVKIALGCKAEIIPSNLVNGEILRPHEILATIEEVA"
     gene            complement(3690..3899)
                     /locus_tag="PLY83_04825"
     CDS             complement(3690..3899)
                     /locus_tag="PLY83_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOV29390.1"
                     /translation="MAKKFDVIINKAWCKGCGLCIAICPKKVLELDAKAKCEPVRMED
                     CIGCRQCENICPDLAITVKERGENA"
     gene            complement(4139..5290)
                     /locus_tag="PLY83_04830"
     CDS             complement(4139..5290)
                     /locus_tag="PLY83_04830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOV29391.1"
                     /translation="MTWKTTAEAVVIGGGINGVSVAYNLAKLGMKDVVVLEKNTVCSG
                     STGRCGAGIRAQWGTEMNCRFGLACVQKFEQLSEELGMDVGLNQSGYLMVAYHQSEFE
                     QLQKNVALQNSLGYMTKIVSHDEAREICPGVAVDDAIGFTFNHRDGHADPFLTTFAYQ
                     EAGKRLGVTFHKHTEVTGIKVKSDKVAGVTTTRGDIDAPIVINAAGPYSQFVAKMAGV
                     EIPNYSERHEIIITEPVEPGVCPPMLMSFSGNYYLQQRPHGSIIGGCSPEGHPADFTL
                     GATWDFLEHMSRVFTKLLPRAKGIRVVRQWSGLYNMTPDRQPIICEADNVKGFYLTCG
                     YSGHGFMFGPVTGEALAQHIILGKSDIPIDMLHYRRFAEGKLLIEPAVV"
BASE COUNT         1024 a         1570 c         1580 g         1189 t
ORIGIN      
        1 cgttccgcgg cggcctcgac gatcttcacg ttagacaacc cgttcttgac tgcattgtcc
       61 cggaggcagt caacggaggg tttccactcc tccaccgcaa ggaccttgga agcccgtttg
      121 gcaaggaaac aggtcagggt gccgacgcct gcgtacagtt cgaggacgtt ttcggttccc
      181 gcctggcagg cctctccgga agccgtttcg taaagacggg cggcctggct cgtgttgacc
      241 tgggaaaagg aagtcgcctc gaacttgaag gtcatatcgc cgaaacgctc gaagaagaaa
      301 gtgcggccgg aaagggcgcg ggttatcggg ccgaagattg cattcccgcc ggagggattt
      361 ccgttcagga caaagcccgc aagggacggg taccgtttcc ggattcggcg cgccagggcg
      421 atcaggctgt cgacgacctg ggaaaccggg aattcccgca caacgagcac aacgagcaac
      481 tcccctgtct cctgtcctcc ccgcaggaca acatggcgca aggttccctt gccggtattc
      541 tcgttgtagg gcatcaaccc ggtctgccca agaccccggc ggatttccga gagcaggccg
      601 ttcaatccgg ccatggccac cgggcagtgt tccacgggaa cgatcctgtg ggtttttttc
      661 cggaaatacc cgatttcgag cagcccatcc cctcccccca ccgggagaga gatcttgttg
      721 cggtagcccc acacgagcgg gcttggacgg acagcttcga attcctcgag gggaacttgt
      781 ctgccggtcc gggaaaaggc gtcggtgatc atctgtaact tcaggtgaag ctggaaaggg
      841 taggatgcgt gctggatctg gcaaccgccg catttgccat gcagggggca ggccggaacg
      901 acccgctccg ggtgcggttt gaggacgttg acgaggcgag ccgtgcaata atcccttttc
      961 tctgttatga cttccgccag gacggtttct ccaggcaaag ccccgggaat aaagacgatt
     1021 ttcccttgcc cgtttcgggc aaggccttct ccctggctat cgattttttc aatcgttaga
     1081 tggagctgtt gtgatggaac ccttctcatt tttcccccgc gttcttgaaa aaggggcggc
     1141 cctgaaggcc gccccgaagt ctggtctgta tcggagagaa ggtcacttct tctccttcat
     1201 catggaatat ccttcttcga aggcctgttc gttcagcttt tcggttccct ttggaacacg
     1261 ggccaggatc gcctttttga ccgaatctgg ccggatcacg ttcgccagct ccaggattct
     1321 cgccgtggca ccaagggcaa ccatgttggt gaccatttcc tttccgagtt tttccttggc
     1381 tgtcctcacg atgggcagca tgtagatatc cgcgtccacc tggggggggt cggaaacgta
     1441 aaagtcgtcc aggatgatcg tcccgccctt tttcgtgtcg tgggcgtatt cttcgcaggc
     1501 tttctgggtg aggatgacct gcagatccgg acgggttgct ttcggatagt cgatcacgtc
     1561 gtgggagacg accacctctg actttgacgc gcctccccgt gcctccggac cgtacgcctg
     1621 gctctggacg gcatagacgt tctcgtaaag ggcggcggct tctcccgtca cgaccgcggc
     1681 gaggataact ccctgcccgc ccgaacccgc aaaacggatt tcaaaacgct cgctcatgaa
     1741 gactaccccc taacccgttc gatcagcttg agataggcct cagtgtattc caggccctcc
     1801 cggtcgacaa attctccaat gacgattttg cccttctttt cctcttcggt catttccttc
     1861 gctttcgcct tgggaatgga gttttccttg aagaaaatga gattgtcgac ggggttggga
     1921 cgcttgtttt tgcgtccgaa ctgggtgtgg caatgggtga tgatctccac gaccgaaaag
     1981 cccttcttct ggatggcctt cttgatgtat tgctcgcaaa gcgccggctg agcgatggtg
     2041 gccctcgcga cataggaggc accggcgccc tcggcgagcc tgcagatgtc aaacggggtg
     2101 tcgattgctc catatggcgc ggttgtcgct ctggccccaa ccggagtggt gggggatgcc
     2161 tgtccgcccg tcattccgta gatgttgttg ttcatgacga tggccgtgat gtcgatgttg
     2221 cgcctgcagg cgtggatgaa gtggttgccg ccgatggccg tgcagtcccc gtcacccatg
     2281 acgtcaatca ctgtcagctc gggcttggcc agcttgatcc ccgtggcgaa ggccagtgaa
     2341 cgtccatggg tcgtatggac ggtgttggcg ttgatgtacc ccggcatccg gcttgaacag
     2401 ccgatcccgg aggcaatgac ggtttcctcc cgggttttcc cgagatcgac cagggagcgc
     2461 aggatcgcgt gcaggatgat cccgtgaccg caacccgggc accagatatg ggggaagaac
     2521 cgggtccgaa gccagggctt gatttcttcg cgtggcatgg ttaggccacc tcctcgatcg
     2581 tagcgagaat ttcgtgcggg cgcaaaatct caccgtttac gagattgctg gggatgatct
     2641 cagccttgca cccaagggcg atcttcactt cggagatcat ctgcccgcaa ttgagttcgg
     2701 ggacgatgac ctttttggcc tgaccggccg cctgaatcag ttccttatcc gggaacggcc
     2761 agagggtgat cgggcggaag tgtcccacgc ggatcccctt ggcacgggct tcgcgaaccg
     2821 cccggacggc tgacctggcg acgcagccgt aggaaacgac caggatttcg gcgccttccg
     2881 tctgctcaag gtcgtacttg acgatctcgt cgcggaaccg ctcgaccttt ctcacgaggc
     2941 gaagatgctt tttcccgatt tcctccggct cgtttgtggg gaacccgtag tcgttgtgcg
     3001 ttagcccggt gacgtgccag cggtagccgt cgccgaagga agccatcgcc ggaacgtcgt
     3061 ccgccggatc ggcgagatag ggcacgaatt cttccgggga aaccttcggc cgtttgcggt
     3121 cgacaatcgg gtaagagccc ggctccggga tgacgacctt ctcgcgcatg tgaccgatga
     3181 cttcgtcgct catgacgaga accggctgcc tgaagcgctc ggccatgttg aaggcgtcga
     3241 tcgtcagttc aaaacactcc tggacggagg aaggggcata ggcgatggtc ccgtggtcgc
     3301 cgtgggttcc ccagcgggcc tgcatgacat cctgctggga aactcgagtt ggcaacccgg
     3361 tcgaagggcc gccccgcatg acgtcgatca cgacgatggg aatctcggcc atgtaggcca
     3421 gcccaaggtt ttcctgcttg agggagaaac cgggcccgga agtggcagtc atggccttgt
     3481 agcccgcgat ggaagcaccg atggtcgcag cgatgcctcc gatctcatct tccatctgga
     3541 tgaagcggcc gttgatcttc gggagttctt ccgccatgac ttccgcgatt tcggaagacg
     3601 gggtgatcgg gtaacctccg aagaaacggc atcccgccgc gatccccccc atggcgatag
     3661 ccatgttgcc ctgccagaaa gccagtttct taggcattct ctccacgctc ctttacggta
     3721 atggcgagat ccgggcaaat gttctcgcac tgccggcagc cgatgcagtc ttccatccgg
     3781 accggttcgc acttggcctt cgcgtcgagc tcgagaacct ttttcgggca gatcgcgatg
     3841 caaagaccgc agcccttgca ccaggccttg ttgatgatga catcgaactt cttagccaaa
     3901 aagctcaccc tccccagaca acacaagtcc gagttccacg cgaggcggaa cccgctcatc
     3961 cgattatgac attcctgaaa acgagaaaca accctaaata gagttcttta ttgtgaatat
     4021 tgctttacaa tgcgtcacct tgatcgaacc aaccgaagat tcattcacaa aatgagggga
     4081 tagcctgggc tatcccctcg accgatagga gcaagcggat cgtttctcgc agtgcccttt
     4141 agacgacggc cggttcgatc agaagcttgc cttcggcgaa acggcggtaa tgaagcatgt
     4201 cgatgggaat gtcggatttg ccgagaatga tgtgctgggc aagggcctct cccgtcaccg
     4261 gaccgaacat aaatccatgt cccgaatacc cgcaggtaag atagaatccc ttgacgttat
     4321 cggcttcgca gatgatgggc tggcgatcgg gggtcatgtt gtacagcccg gaccactggc
     4381 ggacgacgcg aatccccttg gctcggggga ggagttttgt gaagaccctg gacatgtgtt
     4441 ccaggaaatc ccaggtcgcg cccagcgtga agtccgccgg gtggccttcg gggctgcatc
     4501 ctccgatgat ggatccatgg gggcgctgct ggaggtaata gttcccggag aagctcatga
     4561 gcatcggagg gcataccccc ggctccacgg gttccgtgat gatgatctcg tgccgctccg
     4621 agtagttggg aatttccacc ccagccattt ttgcaacgaa ctgggaatag ggtcccgccg
     4681 cgttgatgac gatcggagca tcgatgtctc cccgggttgt cgtcaccccg gccaccttat
     4741 cgcttttgac cttgatcccc gtgacttcgg tgtgcttgtg gaaggtgacg cctagtctct
     4801 tgcccgcttc ctggtaggcg aaggtggtca aaaacggatc cgcatgaccg tcccggtggt
     4861 tgaacgtgaa tccgatggca tcatccacgg caaccccggg gcaaatctcc cgcgcttcat
     4921 cgtgggaaac aattttcgtc atatagccca gcgaattctg aagggccaca ttcttctgga
     4981 gctgctcgaa ttcggactgg tggtaggcga ccatgaggta gccgctctgg ttgagtccga
     5041 catccattcc gagttcctcg gaaagctgtt cgaacttctg gacgcaagcc agcccgaacc
     5101 ggcagttcat ctcggttccc cactgagccc ggatccccgc tccgcagcgt ccggtcgatc
     5161 cagagcagac cgtgttcttc tcgaggacga cgacgtcctt catgccgagc ttcgccaggt
     5221 tgtaggcgac ggaaaccccg ttaatgcccc cgccgattac aaccgcttca gccgttgttt
     5281 tccaggtcat cagttgtctc ctcccttagc gagaacgccg agcttgacgg gtttgacggg
     5341 gggcctgaag gtcccgggct cca
//
LOCUS       DAOOVN010000046         4990 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_9425_length_4990_cov_15.367173, whole genome shotgun sequence.
ACCESSION   DAOOVN010000046 DAOOVN010000000
VERSION     DAOOVN010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4990
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9425_length_4990_cov_15.367173"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="PLY83_04835"
     CDS             complement(<1..811)
                     /locus_tag="PLY83_04835"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOV29392.1"
                     /translation="MTLTIRNLSLRYGEKILFDRIGWTVPDRARVGLVGDNGAGKSTL
                     LKTILGKIQPDSGTIEIPKGARLGYLPQDLVELGGGTVLETLRNRCGVADLEAALAEC
                     EAELASLEADDPKLPEALARHERLVQAHGALDAYAFEARARRVLKGLGFAEGDADRSC
                     ETFSGGWKMRIALASLLLQEPEILLLDEPTNHLDTESMEWLEAFLKNSRCTLVTVSHD
                     RRFLDNVVTTIAELCRGNLTLYPGDYEDFLRQRDEREERLAREAEAQREVIE"
     gene            948..1595
                     /locus_tag="PLY83_04840"
     CDS             948..1595
                     /locus_tag="PLY83_04840"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOV29393.1"
                     /translation="MKHALTVLLLLVFFTFRAGLASAEELDTPPLYDPNSPPVVVSEE
                     PSVPEEAPPLPEPPLVTAQRKAIAALFTRINKSLGAEKAERYAGHVLDAGKLFGIDPL
                     VVASILVRESRANASAKNRSNIGLMQVNWKAHEKNLPTAFPEIRTVKDLFDPRNNILA
                     GAWIFSCYHKSAGGDLSKSLKKYVGGNSGYVEKVLSAYRTLKNDLATLAASAASP"
     gene            1606..2247
                     /locus_tag="PLY83_04845"
     CDS             1606..2247
                     /locus_tag="PLY83_04845"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOV29394.1"
                     /translation="MKEALRRALACLAKAPAVPPEVLGIGSSLLHVSDTPSLFYPKLG
                     RIAEAVKPRLIVHTGDLADEIKLRFPSPDLRLYASKVQALLSVLRATSAERIVLVMGN
                     HDDFSVVKGLCASFGGRFQAFERCARLEFLGLRIAAAHRPEDLWKCVSEDPADFGLYG
                     HDTSVLPPDADGRVYLNGNVKVRVLDLASRTVHSIDYPPSVHDARQKKRKIGL"
     gene            2271..3281
                     /locus_tag="PLY83_04850"
     CDS             2271..3281
                     /locus_tag="PLY83_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29395.1"
                     /translation="MISLMRGGPRLLLLETKNADELERFLDRRMAEHAYDLREAWKKA
                     GEQQTLVIVKDASAEEGPDSANRLEGSRIYVISESTEDLLCALVNERLCSLVDRVQTR
                     QGGLIVRTFGEIDAIVDLLKAELGAVETGGPDLASWPEQQGTTVLFTEKPLGKIIEPS
                     DLHPRRVVLPMKNEVASRLMRRSIVRYVTSGLAGRSWHAVELRLYDKYGLYDLHYRRI
                     MRVLEALEIGLVVGEGWSRDFARVLLELKCYSLKMVTPMEPEAVKRILMGLEYAADGK
                     RILDLDLYEGKRKISWGEAAAGESREAAGLRHREELRARLEPSDREEFDALEAEILSG
                     NW"
     gene            complement(3267..4040)
                     /locus_tag="PLY83_04855"
     CDS             complement(3267..4040)
                     /locus_tag="PLY83_04855"
                     /inference="COORDINATES: protein motif:HMM:NF022655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2993 domain-containing protein"
                     /protein_id="HOV29396.1"
                     /translation="MKRNPKAGFLAVFIALAVATACLASGAASAGAQGISLSNEGRSA
                     LLKAALERKFSPQSMEVRLEGGPDAKGFVKRAVVRAKGAVIDRLRVESLVMEASDLQF
                     NPPEEWDKKLEVKSAGETRSRGVILEKDINAAMAESVKGDKKWKSIRMDLSPRQIKAV
                     GVYETYFLFKLHLVVKLTGRLDASPDGKQIFIRDYKLNVSGLTMPQGLTEDVIEKVQP
                     IIDFRESVFPVSVKKIVMDENSISFVSDPPPLTDASPVP"
     gene            4236..4631
                     /locus_tag="PLY83_04860"
     CDS             4236..4631
                     /locus_tag="PLY83_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II SORL domain-containing protein"
                     /protein_id="HOV29397.1"
                     /translation="MKFEHIIKSADWKTEKHSPVIEAPETVKAGEKFVVDLSVGKEIP
                     HPNTTEHHIRWIKLYFMPEGGKFPFEVANVEFTVHGESVDGPNKGPVTTEPHAKVAMK
                     ITAPGMLIAASYCNIHGLWESAREIKVEA"
     gene            4756..>4990
                     /locus_tag="PLY83_04865"
     CDS             4756..>4990
                     /locus_tag="PLY83_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HOV29398.1"
                     /translation="MRKASLLLAAFALLALMAGTALAAAPFHIGVVTGTVSQSEDDLR
                     GAEALIKNYGDVAKGGMIKHITYPDNFMSEMETT"
BASE COUNT          957 a         1623 c         1496 g          914 t
ORIGIN      
        1 tttcgatgac ctccctctgg gcttccgctt cccgcgccag ccgctcttcc cgctcgtccc
       61 gctggcgcag gaagtcctcg tagtcccccg ggtacagggt gaggtttccc cggcaaagct
      121 ccgcgatcgt cgtcaccacg ttgtccagga agcgccggtc gtggctcacc gtcacgagcg
      181 tgcagcggct gtttttcaaa aacgcctcca gccattccat gctctcggtg tccaggtggt
      241 tggtcggctc gtccaggagc aggatctcgg gctcctgcag gagcagcgac gccagggcga
      301 tccgcatttt ccagccgccc gagaaggtct cgcaggaacg gtccgcgtcg ccctcggcaa
      361 acccgagtcc cttcaggacc cgccgggccc gggcttcgaa agcgtaggca tccagggccc
      421 cgtgagcctg gaccagccgt tcgtgccttg ccagggcctc gggcagcttc gggtcgtctg
      481 cctccaggga agccagttcg gcttcgcatt cggccagggc cgcctccagg tctgccacgc
      541 cgcaccggtt ccgtagggtc tccagcacgg tcccgccgcc cagctccacg aggtcctggg
      601 gcaggtaccc cagccgggcg cccttcggga tctcgatcgt tccggaatcc ggctggattt
      661 tccccaggat ggttttcagc agcgtgcttt tgccggcccc gttgtctcca accaggccca
      721 cgcgggcacg gtccggaacg gtccagccga tgcggtcgaa caggattttt tctccgtagc
      781 gaagggaaag gtttcggatg gtcagcgtca tgagggctat tctccacgga aggcgccgaa
      841 agggcaaggg cagcccgacg gatcgtgtgg cgaccggctt taagccgggg gggttgtcaa
      901 gccttccccg aggggttatc ttttctctgc gaaaggcggg atgcatgatg aaacacgccc
      961 tgaccgttct gcttctcctt gttttcttca ccttccgcgc gggcctggcc tccgcggagg
     1021 aactggatac gccgcccctc tacgacccaa actccccacc ggtcgtggtt tccgaggaac
     1081 cttccgttcc ggaggaggct ccgcctttgc ccgagccgcc cctcgtgaca gcccagcgga
     1141 aggccattgc cgccctcttc acccgcatca acaagagcct cggggcagaa aaggcagagc
     1201 gctacgccgg ccacgtcctc gatgccggga aactttttgg catcgatccg ctggtggtgg
     1261 cctccatcct cgtccgggag tccagggcca acgcctccgc gaaaaaccgg tccaacatcg
     1321 gtctcatgca ggtcaactgg aaggcccacg agaaaaacct cccgacggct ttccccgaaa
     1381 tccggaccgt caaggacctg tttgaccccc ggaacaacat cctggcgggc gcctggatct
     1441 tctcctgcta ccacaagtct gccgggggcg acctgtccaa atcgctgaaa aagtacgtcg
     1501 gcggaaattc cgggtacgtg gaaaaggtcc tctctgccta ccggacgctc aaaaacgacc
     1561 ttgccaccct ggcagcctcg gccgcttccc cctgagactt cacccgtgaa agaagcgctt
     1621 cgccgggcac tggcctgcct ggcaaaagcc ccggcagtgc cgccggaagt ccttggcatc
     1681 ggctcgagcc tcctgcacgt ttcggatacg ccttcccttt tctacccaaa gctcggccga
     1741 attgccgagg ccgtcaagcc ccgcttgatc gtccacaccg gggacctcgc cgacgagatc
     1801 aagctccgct tcccctcgcc ggatttgagg ctgtacgcct cgaaggtcca ggcgctgctc
     1861 tcggtcctgc gggcgacctc cgccgaacgg atcgtcctcg tcatgggcaa ccacgatgat
     1921 ttctccgtcg tgaagggcct ctgcgcctcc ttcggcggaa ggttccaggc cttcgaacgc
     1981 tgcgcccggc tggagttttt gggattgcgg atcgcagccg cccaccggcc cgaagacctc
     2041 tggaaatgcg tttcggaaga tcccgccgac ttcggcctct acgggcacga caccagcgtc
     2101 ctgccgccgg acgccgacgg cagggtttac ctcaacggga acgtgaaggt tcgcgtgctg
     2161 gacctcgcct cgcgcaccgt ccactccatc gactaccctc cctcggtcca cgacgcgcgc
     2221 cagaaaaaaa ggaagatcgg cctctaggga accggaaggg ggaaagagaa atgatctcgc
     2281 tgatgagggg aggaccccgc ctgctcctgc tcgagacaaa aaacgccgac gaactcgaac
     2341 gtttcctgga caggaggatg gcggagcatg cctacgacct gcgggaagcc tggaaaaagg
     2401 ccggcgagca gcagacgctc gtcatcgtga aggacgcctc ggcggaagaa ggcccggatt
     2461 ccgcgaaccg gctggaaggg agccgtatct acgttatcag cgaaagcacc gaagaccttc
     2521 tctgcgccct cgtcaacgag aggctctgct ccctggtcga ccgggtgcag acccgccagg
     2581 gggggctgat cgtccggact ttcggggaaa tcgacgccat cgtcgacctg ttgaaggccg
     2641 agctcggggc cgtggaaacc ggggggccgg acctggcatc gtggcccgag cagcagggga
     2701 ccacggtcct gttcacggaa aaacccctgg ggaagatcat cgagccctcg gacctgcatc
     2761 ctcggcgcgt ggttcttccc atgaaaaacg aggtggcttc ccggctgatg cgccgcagca
     2821 tcgtgcggta cgtcacctcg ggtctggccg ggcggtcgtg gcatgccgtc gaattgcgcc
     2881 tctacgacaa gtacggcctg tacgacctgc actaccggcg gatcatgcgg gtcctggagg
     2941 ccctggagat cgggctcgtg gtgggggagg gatggagccg ggatttcgcg cgggtgctgc
     3001 tcgagctgaa atgctacagc ttgaagatgg tcacgcccat ggagccggaa gcggtcaagc
     3061 gaatcctgat gggactggaa tacgccgccg acggcaagcg catcctggac cttgacctgt
     3121 acgagggcaa gcgaaaaatc tcctgggggg aggccgccgc gggggaaagc cgagaagcgg
     3181 cgggccttcg gcaccgcgag gaacttcgag cccgcctgga accttccgac cgcgaagaat
     3241 tcgacgccct ggaggcggaa atcctctcag ggaactggtg aggcgtccgt caaaggcggc
     3301 ggatcgctca cgaacgagat cgagttctcg tccatcacga tcttcttcac ggaaacgggg
     3361 aacacggact cccgaaagtc gatgatcggc tgcaccttct cgatcacgtc ctccgtcagc
     3421 ccctggggca tcgtcaggcc gctcacgttc agcttgtagt cccggatgaa gatctgcttg
     3481 ccgtcgggcg aagcatccag ccttcccgtc agcttcacca ccaggtgcag cttgaacagg
     3541 aaatatgtct cgtacactcc cacagccttg atctgccggg gcgacaggtc catgcggatg
     3601 cttttccact tcttgtcgcc tttcaccgat tcggccatgg cggcgttgat gtccttctcc
     3661 aggatcacgc cccggcttcg ggtctccccg gcgctcttca cctcgagctt tttgtcccat
     3721 tcctcgggag ggttgaactg caggtccgaa gcctccatga cgaggctttc cacccggagg
     3781 cggtcgatca ccgctccctt tgcccgcacc acggcacgct tcacgaaccc ctttgcgtcg
     3841 gggccgcctt ccaggcggac ttccatcgat tggggggaga acttccgctc cagggcggct
     3901 ttcaacagcg cggatcgacc ctcgttggaa agagaaattc cctgggctcc ggcagaagcg
     3961 gcccccgagg ccaggcaggc cgtcgcgacc gccagggcga tgaaaacggc cagaaaaccc
     4021 gctttcggat ttcgtttcat gttccacccc tcctcttcct ctccccattt tgagccatcc
     4081 gtgatccact gtccacggga gtcttgcgga tccccgaaca ttgaagacac tttctttcaa
     4141 aacgtacgct gattgacaga aagtgcccga aaagggtatt ttaagcaccg agacagttta
     4201 ccgtgattgt cgatggaaaa ggagggattg cagagatgaa gttcgagcac atcatcaaga
     4261 gcgccgactg gaaaaccgaa aagcactccc cggtgatcga ggccccggaa accgtgaagg
     4321 ccggtgagaa gttcgttgtc gacctcagcg tgggaaagga gattccccac ccgaacacca
     4381 cggagcacca tatccgctgg atcaagctgt acttcatgcc ggagggcggg aagttcccct
     4441 tcgaggtcgc caacgtcgag ttcacggtcc acggggagtc cgtggacggc cccaacaagg
     4501 gcccggtcac taccgaaccc catgcgaagg ttgccatgaa gatcacggcc ccgggcatgc
     4561 tcatcgccgc ctcctactgc aacatccacg gcctgtggga aagcgccagg gaaatcaagg
     4621 tcgaagccta gacgcacgac gccgcggggg actgaaccgg attcagtccc ccggttcatt
     4681 ttgccgttga aaacttggtg ttcagctgat atattatggg cagtcacaaa aacttaagaa
     4741 gggggtcaaa ggtacatgag aaaagcgtca ctgctgctcg ctgcatttgc gctcctggca
     4801 ctcatggccg gaaccgccct ggctgcggcg ccgttccaca tcggcgtcgt caccgggacc
     4861 gtttcacagt cggaggacga ccttcgcgga gcggaagccc tgatcaagaa ctacggggac
     4921 gtcgcgaagg gcggaatgat caagcacatc acctatcccg acaacttcat gtccgagatg
     4981 gagaccacca
//
LOCUS       DAOOVN010000047         4340 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_11143_length_4340_cov_15.183664, whole genome shotgun
            sequence.
ACCESSION   DAOOVN010000047 DAOOVN010000000
VERSION     DAOOVN010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11143_length_4340_cov_15.183664"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..167)
                     /locus_tag="PLY83_04870"
     CDS             complement(36..167)
                     /locus_tag="PLY83_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29399.1"
                     /translation="MGTLWIWVLLFCVLAIFVGGIMTILRGILKMRELLRKRPEKER"
     gene            468..2069
                     /locus_tag="PLY83_04875"
     CDS             468..2069
                     /locus_tag="PLY83_04875"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOV29400.1"
                     /translation="MRWDLIIPVLLMYAGVAVLTSVALRRVRDSLDYMLAGRQLGYGL
                     VIPHIIGAFFGAGSTIGVSALAYIWGFGGSWYNMAEALGLWILMAFMARRFWHLGKRL
                     NFVTLPDLLESYYGKGFKAITGLFIALGYMSWVAGQIVGGGRVLQTITGFPLVWGIIL
                     ATALVGFYAGFGGLWGSVFADLVFGSLTVIGAVVIAPVLISQVGGWSGIVSRVPANYL
                     HFFWLGEKNPIWGGIYGWTSVKWFIWYLMVFVPAFAIGQLNIQRIYACKSERVAAGMS
                     TFISAYVMIQPVFFAVFGMVAFALNPNLPSKDAAAPWVMANAMSTPVAVVFLCSVMAT
                     ILSCAAAGLNVSSSSLVRDIWRQKNPNLDELKAGRIASLGVTLISLVLALALPDVVGW
                     LALGFTLMSCSLFIPTAAIVVAKGRFTKWATPKGAIWSSLIGGGAAILWKVLIQVYGM
                     PFKNWDPVFIGLPTSVVLFFIISAMTSNEKIQANETYKARIKTIQEVFDEVRPCFAFE
                     GKDSLIVLASIFVAIFVMPKVLALF"
     gene            2154..3047
                     /locus_tag="PLY83_04880"
     CDS             2154..3047
                     /locus_tag="PLY83_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOV29401.1"
                     /translation="MKEFVLAGCQIASEPMDVDSNVRKGVEWIPKALEVGAELIVFPE
                     TVTTGFNPGCSKEELWDKVDFAGGRITEPIQKAAAKYGVYVIWPSYERGENRGDIYNT
                     AFLIGRNGEIIGRYRKTHPWPGENINGIGWVIPGNEAPAFDTDLGKIGIIICYDGDFP
                     DLSTTIALKGAEVICRPSALLRTYDHWWATNFARAYDNHVYVAAINAVGTDAIKTYYF
                     GHSMIIQPNGWKVAQGRCAEEVVYARLTPEALKTVYGGMTSQQIFDHMTDRNLEVYDV
                     MKDGQSPFEPSKRIPVRKGKA"
     gene            3123..4328
                     /locus_tag="PLY83_04885"
     CDS             3123..4328
                     /locus_tag="PLY83_04885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOV29402.1"
                     /translation="MKGVTEEQRRTISAFLQSCESDLLDFTRKLIRINTVNPPGQEDP
                     VIWTVGELFDRFNIPYTVFPHGDGRSSILGILRGNGEKPGLLMAGHADTVPLGESKWG
                     HPPFGADIEEGKIYGRGASDMKSGVAAIVFAAGALAALGVPIRGDLLVAITAGEETDS
                     LGAREVVNSPWIREAACGLIAEPTDLNILVAEKGALWVKLEVFGVTAHGSMPWQGVNA
                     VEFLAELTAWWKEEWEKNWTHSAHELLGPATISLNRFEGGTASNVVPDHAEAVIDMRT
                     LPGQDHGKILDTLRGQMDLLQQRHPGLRMSLHVLNDRPPLEISKDHPLAVSLREKVSE
                     ITGVDPAFGGAAFYTDASVFGPQTGMPLVICGPGNMATMHKADEYVEIQNLKHAAEIF
                     ACWAVEWLC"
BASE COUNT          891 a         1193 c         1298 g          958 t
ORIGIN      
        1 ttgttgagct ccctcccttt ccactcaccg tgagattacc gttctttttc cggcctcttg
       61 cggagcaatt ccctcatctt cagaatgcct ctgagaatcg tcatgattcc tcccacgaag
      121 attgcgagga cacagaacag tagaacccag atccacaacg tccccattcg ctttgtttgc
      181 cctccctgtg agtcgcgagt gatcaaaacg atcttagcaa gattcctgaa aaccggggtc
      241 gattctgccc tgttacaagg tcacaagaaa agccgaaacc ttgtgaaggc ttaacagggc
      301 gttcgctccc cttcttccct ataatcggct ctgatcccct gtccgggctt atcgcaatac
      361 gatttcagcc ttaatgaaag gagggtaaat tagcgaaagg gattaaatat gtgaaggcag
      421 gcaacccatc tttgattcag tccgatttca cggaggtgaa tggttgaatg cgttgggatc
      481 tgatcattcc agtcttgctg atgtatgcgg gtgtcgccgt tctgacgagc gttgcattga
      541 ggcgagtccg cgattccctc gactacatgc ttgcgggacg tcagcttgga tacggtctcg
      601 ttattcctca cattatcggg gcgttcttcg gtgccggttc gacgatcggc gtttcggccc
      661 tggcctacat ctggggcttt ggaggttcct ggtacaacat ggcggaagcc ctgggcctct
      721 ggatcctcat ggcgttcatg gcgcgccggt tctggcacct gggcaaacgg ctgaatttcg
      781 tgaccctgcc cgacctgctc gagtcctatt acgggaaagg gttcaaggcc atcaccgggc
      841 tctttatcgc gctcggctac atgagctggg ttgccggcca gatcgtcggc ggcggccggg
      901 ttctgcagac catcaccggc ttccccctcg tctggggaat catccttgca acggcgcttg
      961 tcgggttcta cgcgggtttt ggaggtctct gggggtccgt ttttgcggac ctggtcttcg
     1021 gttcgctgac ggttatcggg gctgtcgtga tcgctccggt tctcatcagc caggtcggag
     1081 gatggtcggg catcgtgagc cgcgtcccgg cgaattacct gcacttcttc tggctgggtg
     1141 agaaaaaccc catctggggc gggatttacg gctggaccag cgtcaaatgg ttcatctggt
     1201 acctgatggt tttcgtgccg gcttttgcca tcggtcagct caacatccag cggatttatg
     1261 cctgcaagag cgaaagggtt gccgccggga tgtccacctt catctctgcg tacgtcatga
     1321 ttcagccggt gttcttcgct gtcttcggaa tggtcgcgtt tgccctcaac cccaaccttc
     1381 cctccaagga cgccgcggct ccgtgggtca tggccaatgc catgagcacc ccggtagccg
     1441 tcgttttcct gtgctccgtc atggcgacca tcctttcctg cgccgcggcc ggtctgaacg
     1501 tttccagctc gagccttgtg cgtgacatct ggaggcagaa gaacccgaac ctggacgaac
     1561 tgaaggcggg ccgaatcgct tccttgggag tcaccctcat ctcccttgtc ctggccctgg
     1621 cccttcccga cgtagtcggc tggctggcgc tgggcttcac gctaatgagc tgcagccttt
     1681 tcattcccac ggcagcgatc gttgtagcaa agggacgctt caccaagtgg gcgactccga
     1741 agggggccat ctggagcagc ttgatcgggg gcggcgccgc catcctctgg aaagttctca
     1801 tccaggttta cgggatgccc ttcaagaact gggatcccgt tttcatcggc ctgccgacat
     1861 cggtagtcct gttcttcatc atcagcgcca tgacgagcaa cgaaaagatc caggcaaacg
     1921 aaacctacaa ggcccggatc aagacgatcc aggaggtttt cgacgaggtc cgtccctgct
     1981 ttgccttcga gggcaaggac agcctgatcg ttctggcgtc gatctttgtc gcaatcttcg
     2041 tcatgccgaa agtcctggcc ctgttctagg aaaagatgga ttcgtgaaga ggacgggggg
     2101 agtttcgatg cggctccccc ttccctattt tgaaagcgga ggcgaaatct gaaatgaagg
     2161 aattcgttct tgcaggttgc cagatcgctt cggaaccgat ggatgtggat tccaacgtca
     2221 ggaaaggggt cgaatggatt ccgaaggccc ttgaagttgg agccgagctg atcgttttcc
     2281 cggaaaccgt gaccacgggt ttcaaccccg gatgcagcaa ggaagaactg tgggacaagg
     2341 tcgatttcgc tggcggcagg atcaccgagc cgatccagaa agcagcggcc aagtacgggg
     2401 tttacgtgat ctggccctcc tacgagcggg gggagaatcg gggagatatc tacaatacgg
     2461 ccttcctgat cggcagaaac ggggagatca tcggccgtta ccgcaagact cacccgtggc
     2521 cgggtgaaaa catcaacggg atcggctggg tcattcccgg gaacgaagcc ccggcgtttg
     2581 acaccgacct gggaaagatc gggatcatca tctgttacga cggggatttc ccggacctgt
     2641 cgacaacgat cgccctgaaa ggagccgaag tgatctgccg tccttctgcc cttctcagga
     2701 cttacgatca ctggtgggca acgaatttcg cacgggccta tgacaaccac gtttatgtcg
     2761 ctgccatcaa cgcggtcgga acggacgcca tcaagacgta ctacttcggt cacagcatga
     2821 tcattcagcc caacggatgg aaagttgccc agggccgctg cgcggaagag gtcgtgtacg
     2881 cccgcctgac tccggaggcg ttgaaaacgg tttacggagg gatgacctcc cagcagatct
     2941 tcgatcacat gacggatcgc aatctcgagg tatacgatgt catgaaagat ggacagtccc
     3001 ccttcgagcc ctcgaagaga atcccggtcc gcaaggggaa agcatagacc cagggacgga
     3061 aacgcaagcg agctggaccg gcgaagcccg gtccagttcg cttcggaagg gaggcgtttc
     3121 gggtgaaggg cgtgacagaa gaacaaagaa gaaccatttc ggcctttctt caatcgtgcg
     3181 aaagcgattt gctggatttc actcggaaac tcatccggat caacaccgtc aatcctcccg
     3241 ggcaagaaga cccggtcatc tggacagtcg gggagctttt tgaccgtttt aacattccct
     3301 atacggtttt tccgcacgga gatggccgct ccagcatcct cggaatcctg aggggtaacg
     3361 gtgaaaagcc cggtttgctg atggccggcc acgctgatac ggtgccgctc ggtgagtcga
     3421 agtgggggca cccgccgttt ggtgcggaca tcgaggaagg gaaaatctac gggcgcgggg
     3481 cttccgacat gaaaagcggg gtcgctgcga tcgtctttgc agcgggggca ctcgcggccc
     3541 tgggcgttcc gattcgcgga gacctgctgg tcgcaattac ggcgggggaa gaaacggaca
     3601 gccttggagc gagagaagtg gtgaactctc cctggatccg ggaggccgct tgcggcctga
     3661 tcgctgaacc gacggacctg aacatcctcg tggctgaaaa aggggctctg tgggtaaagc
     3721 tggaggtttt cggcgtcacg gcccatggtt cgatgccgtg gcaaggggtc aatgcggtgg
     3781 aatttcttgc cgagttgacg gcctggtgga aggaagaatg ggaaaaaaac tggacccatt
     3841 ctgcccatga actgctcggc cctgccacga tcagcctgaa ccgctttgag ggggggacgg
     3901 cttcgaacgt tgtccctgat catgcggaag cggtgattga catgagaacc ttgccaggac
     3961 aggatcacgg gaaaatcctg gataccctgc gcgggcaaat ggatcttctc cagcagaggc
     4021 acccggggtt gaggatgtcg ctgcatgtcc tgaacgaccg gccgccgctg gagatttcga
     4081 aggatcatcc ccttgccgtt tccttaaggg aaaaggtgtc ggaaatcacg ggggtcgatc
     4141 ctgcctttgg cggggcagcg ttttataccg acgcgtcggt tttcgggccc cagacaggaa
     4201 tgcccctggt catctgcgga ccgggaaaca tggcgacgat gcacaaggcc gacgagtacg
     4261 tggaaatcca gaacctgaaa catgctgcgg aaatcttcgc ctgctgggct gttgaatggc
     4321 tttgctgaaa accgaaccaa
//
LOCUS       DAOOVN010000048         4241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_11440_length_4241_cov_12.630196, whole genome shotgun
            sequence.
ACCESSION   DAOOVN010000048 DAOOVN010000000
VERSION     DAOOVN010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4241
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11440_length_4241_cov_12.630196"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2515
                     /locus_tag="PLY83_04890"
     CDS             <1..2515
                     /locus_tag="PLY83_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOV29403.1"
                     /translation="GLTVSDVSKAIGRKHVELPGGSIETDRQQVEIRLSGEYDSVESL
                     RTLPVAVKPGGIVRLADVARVEDGFEEKTKSAFLDGKEAILISIQKQRGSNEVAVSDA
                     VLKRVGELQRIAPRGVELHVVSNTADFVRRSMKGVGTNLLQAVLLCSLVMLFFLGTLR
                     ATFVAVVTIPVCLVGSFVFLKALGLSINNLTMLGLSLAVGMVVDATVVVLENTHRHME
                     DGMAAFQASDLGTGEVAFSVLAGAATTMAVFAPIAFLGGIIGRFFYSFGVTVVSTIAL
                     SLILSLTLTPFLCSRILHKDRPGRIASAIERGLVKLDDAYRSVLEKAIAFRWLTLGVA
                     GGLFALGLFFAFNIGTGLYPSDDQGTFNISFELPAGASLAETERVLAQVDRIVRADPS
                     VAYTFGETGTGSGQQPNRGRLFVSLVPRKERPGVKEIMGKMRRSLSGFRDVRFTFAVF
                     GGADLRMTLVGATPEQLMEISGKVLHELEKDGRLTDLDTDVRLDKPQLNVTLNRARTD
                     DLDIDIRDLSTEVQAYFGGAKAGVFKEGGFRYDIRLMAEGKLRKSPLDVEQVPVKTGS
                     GKIVRLPGLALVERVKAPSVIKRYNRQSALQISGNVVPGFSTGEGIALVRQTVRKFLP
                     ADGSVSVLATGRSRNQEQDFRRLLGALAVAVVLVYTVMVVQFESFLHPFTVMFSLPLL
                     TPGAFGLLFLAGNNLDMMSFMGIILLVGIVVNNAILLVDFINQHRARGMDKVQAVISA
                     APLRLRAILMTAVSTMAGSIPVALKLSEGAEFRQPMAVAVVGGMFTSTFLTLLVIPVV
                     YLVLDDALETSQRLRRWFRIARARHGLAGSLRRLMPKGK"
     gene            2586..3884
                     /locus_tag="PLY83_04895"
     CDS             2586..3884
                     /locus_tag="PLY83_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOV29404.1"
                     /translation="MSLPAIVGMSVQASYNLVDAFFIGRGVGPLALAGTAVVFPLQLF
                     AVGVGSMGGVGTASLVSRSLGAGDQGRANRALGTLVTLALSAGIAASFLGRLALLPLL
                     DLLGARGEILPFARDYMAILLFGLPLQVLGLGLSSVLRAEGNARTPMTAMILSAAVNT
                     LLDALFIFGLGWGIAGAAWATVLSQGFVAGWLGGRLLSGKSVLKPKAVDLAPDPGIAR
                     EILAVGFSELAELTAASFMVTVVVHSMARYGSPLAVAAYGVVSRILSFAYLPLFGVAQ
                     GLQPILGYNYGAGLWSRARRAILVSTITAATISLAAFLLLQLLAGPLFGIFTGSPALR
                     DLGARSLRTMTLGFFCVGFHIIGASLFRALGKAIPALFLSLSAEVILFLPLVLVLPRF
                     WGLEGLWLSYPASDLLSALVTGAVSLRQLRELKEREKKGV"
     gene            3937..>4241
                     /locus_tag="PLY83_04900"
     CDS             3937..>4241
                     /locus_tag="PLY83_04900"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOV29405.1"
                     /translation="MTYRSREKELFTSAGGASRRAIYKGCGYDDVDLARPLIGVVNPG
                     NDAGLGHTHLDKLAESVKAGIWQAGGTPFEFRTIATCGAIPIGTPHMRYELVIRDVIA
                     "
BASE COUNT          672 a         1292 c         1412 g          865 t
ORIGIN      
        1 ggggctgacg gtttcggacg tttcgaaggc gatcggccgg aaacacgtcg aactgcccgg
       61 agggagcatt gaaaccgacc gtcagcaggt cgagatccgc ctttccggtg aatacgattc
      121 cgtggaaagc ctcaggacgc tcccggtggc ggtgaagccc ggcggcatcg tgcggctggc
      181 cgatgtggcc cgcgtggagg acggattcga ggaaaagacc aagagcgctt ttttggacgg
      241 caaagaggcg atcctcatct ccatccagaa acagcggggt tccaacgagg tggccgtttc
      301 cgacgccgtc ctgaagcggg tcggggaact gcagcgcatt gctccccgcg gagtggagct
      361 ccacgtggtt tccaacaccg ccgatttcgt tcggcggtcc atgaagggcg tgggtaccaa
      421 cctcctgcag gccgtcctgc tctgttccct ggtgatgctt ttcttcctgg ggacgctcag
      481 ggcgacgttc gtggcggtcg tcacgattcc cgtgtgcctg gtggggagct tcgtcttcct
      541 gaaagccctg ggactctcga tcaacaacct gacgatgctg gggctttctc tggcggtcgg
      601 gatggtggtc gacgcgacgg tcgtggttct ggagaacacc caccggcaca tggaggacgg
      661 catggcggct ttccaggcca gcgacctggg aacgggggaa gtggccttct ccgtgctggc
      721 gggagccgcg accacgatgg ccgtctttgc cccgattgcc tttcttgggg gaatcatcgg
      781 ccggtttttc tactccttcg gggtgacggt ggtgagcacc atcgccctgt cgctgatcct
      841 gtccctgacg ctgaccccgt ttctctgctc acggatcctg cacaaggacc gtcccggccg
      901 gatcgcctcg gccatcgagc ggggactcgt gaagctcgac gacgcgtacc gatcggtcct
      961 cgaaaaagcc atcgccttcc ggtggctcac cctgggggtt gccggcggcc ttttcgcgct
     1021 ggggctgttc tttgccttca acatcggaac gggactgtat ccgtcggacg accaggggac
     1081 cttcaacatc agcttcgagc ttcctgccgg ggcctccctg gcggaaaccg aacgggtgct
     1141 ggcccaggtc gaccggatcg tacgcgcgga cccctcggtg gcctacacct tcggggaaac
     1201 gggaacgggt tccgggcagc agcccaaccg cgggcgcctt tttgtcagcc tggttccccg
     1261 aaaggagagg cccggcgtca aggagatcat gggaaagatg cggaggagcc tgtcgggttt
     1321 ccgggatgtc cgcttcacct ttgctgtttt cgggggagcc gacctgagga tgacgctggt
     1381 cggcgcaacc ccggagcagc tgatggagat cagcggcaaa gtcctgcatg agctggaaaa
     1441 ggacgggcgc ctcacggatc tcgacaccga cgtgaggctt gacaagcccc agttgaacgt
     1501 gacgctgaac cgggcccgta cggacgacct ggacatcgat atccgcgatc tttccaccga
     1561 ggtccaggcc tatttcggcg gggcgaaggc gggggtcttc aaagagggcg ggtttcggta
     1621 cgacatccgg ctgatggcgg aaggcaagct gagaaagagc cccctggatg tcgagcaggt
     1681 cccggtgaag accggttcgg ggaagatcgt gcggctcccg ggccttgccc tcgtggagag
     1741 ggtgaaggcg cccagcgtga tcaagcgcta caaccgccag agcgccctgc agatcagcgg
     1801 aaacgtcgtt cccgggtttt ccaccgggga ggggatcgcc ctggtccgtc aaacggtccg
     1861 gaagtttctt cccgccgacg ggagcgtctc ggtcctggcc acgggacggt cccggaacca
     1921 ggagcaggat ttcaggaggc ttttgggcgc ccttgccgtc gcggtcgtcc tggtgtacac
     1981 cgtcatggtc gtccagttcg agtccttcct tcaccccttc acggtcatgt tctccctgcc
     2041 gttattgacc cccggagcct tcggcctgct tttcctggcc gggaacaacc tcgacatgat
     2101 gagcttcatg gggatcatcc tcttggtggg gatcgtggtg aacaacgcga tcctgctggt
     2161 ggacttcatc aaccagcatc gggccagggg aatggacaag gtccaggcgg tgatcagcgc
     2221 ggctccgcta cggcttcggg cgatcctgat gaccgccgtt tcgacgatgg cgggttcgat
     2281 tccggtagcc ctgaagctga gcgagggcgc tgaattccgg cagcccatgg ccgtggcggt
     2341 tgtggggggc atgttcacgt cgacgtttct gaccctcctg gtgatcccgg tggtctacct
     2401 ggtgctggac gatgctctgg aaacctcgca gcggctgagg cggtggtttc gcatcgcaag
     2461 ggcgaggcac gggcttgcgg gatcccttcg gaggctgatg ccgaagggga aatgatcgga
     2521 ggcggttttc cctgagcgaa acggaatttc tcggcatcga tccggtcgga aggcttcttt
     2581 tcaggctgtc ccttccggcg atcgtgggaa tgagcgtcca ggcttcctac aacctggtgg
     2641 atgccttttt catcggccga ggggtgggac cccttgcgct ggccggaacg gccgtcgttt
     2701 tccccctcca gctttttgcc gtcggggtcg gctccatggg aggagtggga acggcttcgc
     2761 tggtttcccg aagccttgga gccggcgatc aaggacgggc aaaccgcgcc ctcggaacgc
     2821 tggtgactct cgccctttcg gctgggatcg cggcgtcctt ccttggcagg ctggcccttc
     2881 tccccctcct ggatcttctg ggtgcccgag gggagatcct tccgtttgcc cgggattaca
     2941 tggcaatcct gcttttcggg ttgcccctgc aggtcctggg tctcgggctg agcagcgtcc
     3001 tccgggcgga aggaaacgcc cggaccccga tgaccgcgat gattctctcg gccgcggtca
     3061 acacgctcct ggacgcactt ttcatcttcg gcctcgggtg gggaattgcc ggggctgcct
     3121 gggcgaccgt cctctcccag gggttcgtgg cgggctggct cggaggccgc ctcctgtcgg
     3181 ggaaaagcgt cctgaagccg aaggcggtcg acctggctcc tgaccccggg atcgccaggg
     3241 agatcctggc ggtgggattc tcggagcttg ccgaactgac ggcggccagc ttcatggtca
     3301 cggtggtggt tcactccatg gcccgttacg gttcccccct ggcggtcgcg gcctacgggg
     3361 tggtaagccg gatcctttcc ttcgcgtacc tgcccctttt cggagtcgcc caggggcttc
     3421 agcccatcct ggggtacaac tacggggccg ggctctggag ccgcgcgcga agggccatcc
     3481 tcgtttccac gattaccgcg gcgacgatct cgctggcggc cttcctgctt ttgcagcttt
     3541 tggccggtcc ccttttcggg atcttcaccg gatcgccggc cctgagggac ctcggcgcca
     3601 ggtccctcag gacgatgacc ctcgggttct tctgcgtggg gttccacatc atcggggcca
     3661 gccttttcag ggccctcggg aaggcgatcc cggcactctt tctctccctt tcggcggagg
     3721 tgatcctctt cctgccgctg gttttggttc tcccgcgttt ctggggactt gaggggctgt
     3781 ggctttcgta tcccgcgtcc gacctccttt ctgccctcgt cacgggagcg gtttccctgc
     3841 ggcagctgcg ggagctcaaa gagcgggaaa aaaagggcgt atgataggat ttttttgaaa
     3901 aaaaccgagc tgcggaatcg agagggggaa aaacaggtga cctaccgttc cagggaaaaa
     3961 gaactgttca cgtctgcagg gggtgcctcg agaagggcca tctacaaggg atgcggatac
     4021 gacgacgtcg atcttgcccg ccccctgatc ggggtcgtca atcccggcaa cgacgccggc
     4081 ctcgggcaca cccacctgga caagctggcc gaaagcgtca aggccgggat ctggcaggcg
     4141 ggaggaaccc ccttcgagtt ccggaccatc gcgacctgcg gggccatccc catcgggacg
     4201 ccccacatgc ggtacgaact ggtcatccgg gacgtgatcg c
//
LOCUS       DAOOVN010000049         3856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_12846_length_3856_cov_12.899237, whole genome shotgun
            sequence.
ACCESSION   DAOOVN010000049 DAOOVN010000000
VERSION     DAOOVN010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12846_length_3856_cov_12.899237"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..389
                     /locus_tag="PLY83_04905"
     CDS             174..389
                     /locus_tag="PLY83_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29406.1"
                     /translation="MRLLTLLFSVLTLASFGRALAGFGGFLFGRGRVDYIVQGLALGC
                     VFTAAAFWAWRRYLREIDAEERTPPLE"
     gene            complement(404..910)
                     /locus_tag="PLY83_04910"
     CDS             complement(404..910)
                     /locus_tag="PLY83_04910"
                     /inference="COORDINATES: protein motif:HMM:NF015742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HOV29407.1"
                     /translation="MDALQEVLEGREGRARTQEQLLKAGLTVVQVSLNIPGFPKRLIN
                     DLFCIEKTSWLVRERCLSLGWGIVQEVGLLNGAGYAVLLGVGPGADPKRLKQTCLTLE
                     ENLAWGRAIDLDVRGQRGIIGRGILGMPPRRCLLCGEEAKQCSREGRHDGKLLRKIVE
                     ELIRNCAP"
     gene            complement(910..2262)
                     /locus_tag="PLY83_04915"
     CDS             complement(910..2262)
                     /locus_tag="PLY83_04915"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOV29408.1"
                     /translation="MLEIPIHRESGIPLYFQVAEHLRRLVSSGALPPGTRLPGTRMLS
                     GELDVSRTTVMQAYALLESEGIVRLEGRRGAFVIGECEESESQQPKEGILDLASGLPS
                     MDLVPVKSISRALREILRRFPEAALLGSPPEGLESLRRVLVSHAAARGIPASWENLFV
                     TAGLQEGLATLLKTLALSGTQTLWVEPLTYPDVVLMARNECLEVRCLPENPAEIPRVV
                     SGMKPSDALYLIPSFHNPLGRTLGLGLRREILAETVRTGTWVIEDDAYGELRYGEESV
                     PALKSLPEAERVLYLGSFSQVLFPGLRFGYMLLHPSLADVAGAVRKIRTGGASSLVQM
                     LVANLIETGDLSRALDAARRQMAIRMEALTGAISRALPGYPFLRPEGGIYLWLETPGI
                     AGKDAVSKAADRGVRMSPGEEFSVSGKRVEAVRLSISRLGAQALAGAVCVMASAWGER
                     "
     gene            2344..3240
                     /locus_tag="PLY83_04920"
     CDS             2344..3240
                     /locus_tag="PLY83_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29409.1"
                     /translation="MKTRNVTPRLQEGDLLVFDVDGVLIDTRRSYPLVIRAAIQLGWR
                     KMLGLKTDCTGFSFEHFRVSKRHPGFNDDYDIAWALLSVAAARARVSKDPRLSRSMPS
                     PEEWESTLKEFPPADVVQHTCSRFGDPVGRDRFRDVCEELYLGEHFSGSEKFTALKRG
                     LWHQEKPLLDVHWSELPLPVAIYTGRFLPELKLALIRLGWEDFPPDRVVTPETGISKP
                     SPDGFSRLCQVTGTRFPIYFGDTESDRESLRRFGRGVFVSIGGMLPEEPLRFETVAEA
                     LAALGFWNGKVSAGSGSTGCAR"
     gene            complement(3203..>3856)
                     /locus_tag="PLY83_04925"
     CDS             complement(3203..>3856)
                     /locus_tag="PLY83_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HOV29410.1"
                     /translation="RAYLESARVSGGKNGAVVVNCNPFTLGHRFLIERAADSCDWLFV
                     IVVETDLSLFPFKDRYALVREGTSHLPNVTVLKSDAYAVSPATFPTYFLRDKNSEETA
                     SIQAKLDVTLFANLFVPALDIRVRFVGTEPYCEVTEAYNEAMKTILPGRGVELFEIPR
                     YALEDGTIVSASTVRERIRARDWQSVRRLVPDVTWNYLNSPEAAGVIARIQSSRTRH"
BASE COUNT          732 a         1200 c         1159 g          765 t
ORIGIN      
        1 gaagcgcgcc tctctcatcg atccacaaac ctcccgtcat cctgcatacc gttccatttt
       61 gtaccacttg ggtacaatct tatcacgcgg ttaggttgca cacaacaaac gatttcccgg
      121 gggtcatgta tgctattctt ggagggtcta tctgcattgt tgggggattt caggtgagac
      181 tgttgacgtt gctcttttcc gtcttgaccc ttgccagctt cggccgggca cttgcgggtt
      241 tcggggggtt cctgttcggc aggggacggg tcgactacat cgtccaggga ctggccctgg
      301 gctgcgtgtt cacggccgcc gctttctggg catggaggcg ctatcttagg gagatcgatg
      361 ccgaagagag gaccccgccc ctggaatgag gacaggatct tctttaaggg gcacagttcc
      421 ggatcagttc ctcgacgatt ttcctcaaca actttccgtc gtggcgcccc tcccggctgc
      481 actgcttggc ttcctccccg caaagcaggc accttctcgg cggcattcca agaatcccgc
      541 ggccgatgat gccccgctgg cctcgaacgt ccaggtcgat ggccctcccc caggcaaggt
      601 tctcctccag ggttaggcat gtctgcttga gccgtttcgg atctgcacca ggtccgaccc
      661 cgagcaggac cgcatatccg gccccgttca gaaggccgac ctcctgaacg atcccccagc
      721 cgagggaaag gcatcgctcc cgaacgagcc aggaggtttt ttcgatgcag aagaggtcgt
      781 ttatcagcct ttttggaaac ccggggatgt tgagggaaac ctggaccacg gtcaatccgg
      841 ccttcagaag ctgttcttgc gtcctcgccc ttccttccct tccctcgagg acctcctgca
      901 gggcatccat cagcgctcgc cccatgccga agccatgaca caaacggccc ctgccagggc
      961 ctgcgctccg agccggctga tcgaaagccg gaccgcttcg acgcgtttac cggaaacaga
     1021 aaactcttcc ccgggagaca tgcgaacccc ccggtctgcg gcctttgaaa ccgcatcttt
     1081 cccggcgatg ccgggagtct ccagccagag gtagatgccc ccctccgggc gaaggaaggg
     1141 atatcccgga agcgctcttg aaatcgcgcc ggtcagcgct tccatcctta tcgccatttg
     1201 ccgccgggcc gcatcgagcg cccgggaaag gtcgcccgtc tcgatcaggt tcgccaccag
     1261 catctggacc agcgaagagg ccccccctgt ccggatcttt cgaaccgcac ccgctacgtc
     1321 cgccagcgag gggtgcagaa gcatgtaccc gaaacggagg cccgggaaga gaacctggct
     1381 gaaggatccc aaatacagga cccgctccgc ctctggaagg gatttcaggg ccggaacgct
     1441 ctcttcgccg taccggagtt ccccgtaggc atcgtcctcg attacccagg tccccgtgcg
     1501 aaccgtttcg gcaaggatct cgcgcctcaa accaagaccc aaggtccggc caagggggtt
     1561 atgaaagctt gggatcaggt acagggcgtc tgacggtttc atcccggaca cgacccgggg
     1621 aatttcggca gggttctcgg gcaggcagcg gacctccagg cactcgtttc gggccatcag
     1681 gaccacgtcc gggtaagtca gcggttcgac ccaaagtgtc tgtgtccccg aaagggcaag
     1741 cgtcttcagc aaggtcgcaa gcccctcctg gagtccagcc gtgacgaaaa ggttttccca
     1801 cgaagccggg atcccgcggg ccgcggcgtg gctgaccaga acacgccgaa gcgactcgag
     1861 cccttccggg ggagaaccca gaagggcagc ctcgggaaac ctgcgaagga tctccctcaa
     1921 ggctcgggag atcgatttca ccgggaccag gtccatcgaa ggcagcccgg atgccaggtc
     1981 caggatccct tctttgggtt gctgggactc gctttcctcg cattccccga tgacgaaggc
     2041 ccctctcctt ccctcgagcc ggacgattcc ttcgctctcg agaagggcat aggcctgcat
     2101 taccgttgtc cggctgacat ccagctcccc ggaaagcatt cgggttcccg gaaggcgtgt
     2161 gcccggcgga agggccccgc tggaaacgag acgccgcaaa tgttctgcca cctggaaata
     2221 gagaggaatc ccgctttcgc ggtgaatcgg gatttcaagc aagagttcgg cccccccggc
     2281 cctcatcgtg tatgcttatg ctttagattg tagcggactt ttccggagaa aggcggcagg
     2341 gatttgaaaa ccagaaacgt aacgcccagg ctccaggagg gcgatctgct tgtttttgac
     2401 gtggacggcg tcctgatcga cacgagacgg tcctatcccc tggtgatccg ggcggccatc
     2461 cagttgggat ggcgcaagat gcttggcctg aagacggatt gcacgggttt ttccttcgag
     2521 cacttccggg tcagcaaacg gcatccgggg tttaacgacg actacgatat cgcctgggcg
     2581 cttctttccg tcgcagcggc ccgagcccgg gtttcgaaag acccgaggct ttcccgttcc
     2641 atgccctccc ccgaagaatg ggaatcgacc ctcaaggaat tccccccggc ggacgttgtc
     2701 cagcacacct gttccaggtt cggggacccg gtcggccgcg accgcttcag ggacgtgtgc
     2761 gaggagttgt atctcggaga acattttagc gggagcgaga agttcaccgc tctgaaaagg
     2821 gggctctggc accaggaaaa accgctcctg gacgtgcact ggagcgaact tccccttccc
     2881 gtggccattt acacgggacg gttccttccc gagctgaaac tcgccctgat ccgcctgggt
     2941 tgggaggact ttccgccgga ccgggtggtg actcccgaaa cgggaatcag caagccttcc
     3001 cccgacgggt tctcccgtct ttgccaagtc accgggacgc gttttcccat ttatttcggg
     3061 gataccgaaa gtgaccggga aagcttgcgc cggttcggcc ggggtgtttt tgtgtccatc
     3121 ggggggatgc ttccggaaga gccccttcgt ttcgaaaccg tggctgaagc tctcgcggcc
     3181 ctcgggttct ggaacggaaa ggtcagtgcc gggtccggct cgactggatg cgcgcgatga
     3241 ctcctgcggc ttccggggag ttgaggtagt tccaggtcac atccgggacc aggcgacgga
     3301 cactctgcca gtcccgggcc cgaatccttt cccggaccgt ggaggcggaa acgatcgtgc
     3361 cgtcctccag ggcatagcgg ggaatctcaa aaagctcgac ccccctcccg ggaaggatgg
     3421 tcttcatcgc ctcgttgtag gcttccgtga cctcgcagta gggttccgtc ccgacgaaac
     3481 ggacacggat gtcgagtgcc gggacgaaca ggtttgcaaa gagggtcacg tccagtttcg
     3541 cctggatcga agcggtctct tcggagtttt tgtccctgag gaagtaggtg gggaacgtcg
     3601 cgggagagac tgcgtacgca tcgcttttca gaacggtcac gttcggtaga tgcgaggtcc
     3661 cctctcgaac cagggcgtaa cggtccttga aggggaaaag ggagagatcc gtctccacca
     3721 cgatgacgaa aagccagtcg caggaatcgg cggcgcgttc gatcaggaag cggtgcccga
     3781 gcgtaaaggg attgcagttg accacaaccg ccccgttctt gcctccggaa acccgggcac
     3841 tctccaggta ggcccg
//
LOCUS       DAOOVN010000050         3785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_13141_length_3785_cov_16.570241, whole genome shotgun
            sequence.
ACCESSION   DAOOVN010000050 DAOOVN010000000
VERSION     DAOOVN010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13141_length_3785_cov_16.570241"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..1506
                     /locus_tag="PLY83_04930"
     CDS             88..1506
                     /locus_tag="PLY83_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005416 - amino acid:monoatomic cation
                     symporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid carrier protein"
                     /protein_id="HOV29411.1"
                     /translation="MLEKIMTSIADFLWGWPLIIGVLGYGLVFTVTSGAFQFTYFGTI
                     MKQTFGRLLRKHEVKAEGEVKGVLSAFQAISIAVGGTVGAGAGNIGGVATAIAVGGPG
                     AVFWMWMAALGGMLIKMVEVTLAVHYRQKDEEGLPFGGPMYYIHEGLGIERKMMGVSK
                     VLNFLFIPCMLVGVLITMQCYNVSEALASTFKVNQSLMALFYTILVYLMIAGGLKKLG
                     QIASRIVPFMCILYVVADLGIILARIDQVGTCFSLILSGAFSGTAATGGFAGAAVAMA
                     IKKGLARAVFSNEAGWGTSPMIHSTATTDHPIRQGMWGVFEVFVDTIIVCSITALCIV
                     MTGQWSSGLKGATLTLSAFNAVYGNVGQILLTVSLFLFGITTTTGWYTYYDIILRYVL
                     REKVELKNRILTAYKWFYPIPGLALVLYAAYRGMPADIVWVFADVASGAPTFINLFAI
                     LLLTPKFLELLRDFKARHLGVG"
     gene            1726..3393
                     /locus_tag="PLY83_04935"
     CDS             1726..3393
                     /locus_tag="PLY83_04935"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HOV29412.1"
                     /translation="MGRFGRAVMPVLLLLTVFCGPCSAETVTSADRVERILRSLSLEA
                     KAGQVLMGFFRGDSVTPPLRSMIGRVRPAGMILYSSTGNIGSPEQVADLTRSLQEAAV
                     SAGGLPLFLAVDQEGGRVNRLTRGVTVFPGNMPLGAAGDPELASKAARVTARELRILG
                     INANFAPVADVLTNPFNPVIGTRSFGSDPALVDRMATAMIAPAAEEGVLCVAKHFPGH
                     GNTSVDSHFGLPVDDSPLETLEKVHLAPFAALVKAGVPAIMTAHVAMPGIDRGNPLPA
                     TLLPKSYEILREEMGFSGLTVTDSLGMKALDRYWSIEETAVLALQAGADLLVLGADPG
                     HEPEELEKIHRALVIAVQTDRISRSRLDEAVRRVLRAKETLKVLDDPMPKTERMGELT
                     SPESLALAETIARRALTVVWNRFGPFPAAEADKPKILLWPLEKAGQVSSLLADSAGMK
                     PLFVSLSLRPEDFEGILEGIEGEEAVYVWAGDLGKSPAWKELAQRIGGRTVLLAVGSP
                     ASLSLCPGAGAAVATYSETPVSLKVLGDFLRAPWSPTGKLPVALPEL"
     gene            complement(3390..3722)
                     /locus_tag="PLY83_04940"
     CDS             complement(3390..3722)
                     /locus_tag="PLY83_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOV29413.1"
                     /translation="MEKRGNLFENREAGREAESFQALLELPALRIERIASFGQASPPG
                     FWYEQEEAEWVVLLEGESCLEWENGRRLRMQPGDWVFIPPGERHRVAWTAPGRPTLWL
                     AVFSRFSC"
BASE COUNT          681 a         1170 c         1130 g          804 t
ORIGIN      
        1 ttattgtttc acaaaaaggt ggtgatttat tttcgatgaa gcaatagccc cgccgttttt
       61 ttcattcttt ttaaggaaga ctgcgacatg ctcgaaaaaa tcatgacttc aattgccgat
      121 ttcttgtggg ggtggccgct gatcatcgga gtcctaggtt acggcctggt tttcacagtg
      181 acgagcgggg ctttccagtt cacgtatttt gggacgatca tgaaacagac cttcggccgg
      241 ctcctccgga agcatgaagt gaaggccgaa ggagaggtca agggggtcct ctcggccttc
      301 caggcgatca gcatcgcagt cgggggaacg gtcggggccg gggccggcaa catcggcggg
      361 gtggcgaccg ccatcgccgt gggcggtccc ggggcggttt tctggatgtg gatggccgcg
      421 ctgggaggca tgctcatcaa aatggtcgaa gtcaccctcg cggtccatta ccggcaaaag
      481 gacgaggaag gcctcccctt cggcggcccg atgtattaca tccacgaggg actcggcatc
      541 gagcgcaaga tgatgggcgt ttccaaggtc ctgaatttcc tcttcatccc ctgcatgctc
      601 gtcggcgtcc tgatcaccat gcagtgctac aacgtatcgg aggccctggc ctcgaccttc
      661 aaggtcaacc agtccctcat ggccctcttc tacacgatcc tcgtgtacct catgatcgcc
      721 ggcgggttga aaaaactcgg ccagatcgcc agccggatcg ttccgttcat gtgcatcctc
      781 tacgtggtgg ctgacctggg catcatcctg gccaggatcg accaggtggg aacctgcttt
      841 tccctgattc tctccggtgc gttctcggga acggcggcaa cgggcgggtt tgcgggagcg
      901 gccgttgcca tggcgatcaa gaaggggctc gcccgggcgg tcttcagcaa cgaagccggc
      961 tggggcacct ctccgatgat ccactccacc gcgacgaccg accatcccat ccggcagggg
     1021 atgtggggcg tttttgaagt tttcgtggac accatcatcg tctgctcgat cacggccctg
     1081 tgcatcgtca tgaccggcca gtggagctcc gggctcaagg gagcgacact gacgctttcc
     1141 gccttcaacg ccgtctacgg gaacgtcgga cagatcctgc tcacggtttc cctgttcctt
     1201 ttcgggatca cgacaacgac gggctggtac acctattacg acatcattct ccgctacgtt
     1261 ctccgggaga aggtcgaact caaaaaccgg atcctgaccg cctacaagtg gttctatccc
     1321 atccccggcc tggcccttgt tctttacgcg gcctacaggg gaatgccggc ggacatcgtg
     1381 tgggtttttg cggacgtggc gtccggggcc cccaccttca tcaacctctt tgccatcctt
     1441 cttttgaccc cgaagttcct cgagctgcta agggatttca aggcccgcca cctcggtgtc
     1501 gggtaaatcg atcccaactg tagggtcttc ttcgacaacg ggcgcaaaga caagtaagcc
     1561 agtggagcgt tcctcggggg aagcagtacc ggcttccccc gttttttgtc tcttctgata
     1621 ttattaattt aattagctgt tgcttctctt tattattgat agagttgttg tagaatgaac
     1681 tttatgagat cctgattgat attgaactgg aaaggacggg ttttcatggg acgtttcggg
     1741 cgcgcggtca tgccggttct tcttttgctg acggttttct gcgggccgtg cagcgcggaa
     1801 accgtcacct ccgccgatcg cgtcgaacgg atcctcagaa gcctgtctct cgaggcgaag
     1861 gccgggcagg tcctgatggg attcttccgg ggagactcgg tcaccccccc gctccgttcg
     1921 atgatcggcc gggtgcgccc agccggaatg atcctgtact cgtcaaccgg gaacatcggc
     1981 agcccggaac aggtggccga tctgacccgt tctctccagg aagcagcggt ttcggccggc
     2041 ggcctccctc tttttcttgc tgtcgaccaa gaaggcggtc gggtcaaccg gctaacgagg
     2101 ggagtcacgg tctttcccgg caacatgccc ctaggggctg ccggcgatcc cgagctggcc
     2161 tcgaaggcag cccgcgtcac ggcaagggaa ctccggatcc tgggcatcaa cgccaatttc
     2221 gcccccgtgg ctgacgtgct gaccaaccct ttcaaccccg tgatcggtac gcgatccttc
     2281 ggatctgatc ccgcgctcgt ggaccggatg gcgacggcca tgatcgcccc ggcggcagag
     2341 gaaggggttc tctgcgtggc aaagcatttt cccgggcacg gaaacacgtc ggttgactcg
     2401 catttcggcc tgcccgtgga cgacagcccg ctcgagaccc tggaaaaggt gcatcttgcc
     2461 ccctttgcgg ccctggtcaa ggcaggtgtt cccgcgatca tgacggccca cgtggccatg
     2521 cccgggatcg acaggggcaa cccgcttccg gccaccctgc tgccgaaatc ctacgagatc
     2581 ctgcgggagg aaatggggtt ttcgggcctg acggtgacgg attccctcgg catgaaggcg
     2641 ctggaccggt attggtccat cgaggaaacc gccgtcctgg cgctgcaggc cggagccgac
     2701 cttctcgtct tgggggcgga tccaggccat gaaccggaag agctggagaa aattcatcgg
     2761 gccctggtca ttgcggtcca aacggaccgg atttcccgct cgcgcctcga cgaagcggtc
     2821 cgtcgggtgc tccgagcgaa ggaaacgctg aaagtcctcg acgatccgat gccgaaaacc
     2881 gaacggatgg gagaactgac ttcccccgag agcctggctt tggccgaaac catcgcgcgg
     2941 cgggccctga cggtggtctg gaaccgcttc ggccctttcc cggcagccga ggcagacaaa
     3001 ccgaaaatcc tcctctggcc cctggagaag gccggccagg tgagttccct gctcgcagac
     3061 tccgcgggga tgaagcccct gttcgtttcc ctttccctgc gtcccgagga tttcgagggg
     3121 atcctggagg gaatcgaagg agaagaagcg gtctacgtct gggccgggga cctcggaaaa
     3181 tcccctgcat ggaaagagct ggcccagcgc attggcgggc gcacggtcct gcttgcggtc
     3241 ggctctcccg cctccctttc tctttgcccc ggggccggcg ctgcagtggc cacctactcg
     3301 gaaacccccg tttccctcaa ggtgctggga gatttccttc gcgccccctg gtcccccacg
     3361 gggaaattgc cggtagctct gccagagctc tagcaggaaa acctcgagaa gaccgccagc
     3421 cagagggtgg gtctgccggg ggccgtccag gccacgcggt gccgctcccc tgggggaata
     3481 aaaacccagt caccgggctg cattcgcagg cggcgcccgt tctcccattc caggcaggat
     3541 tcgccttcca ggaggacgac ccattccgct tcttcctgtt cgtaccagaa ccccggcgga
     3601 gaagcctggc cgaaagaggc gatccgctcg atcctcaggg ccggcagctc cagcagggcc
     3661 tggaaggatt ccgcctctct tcctgcctcc cggttttcaa aaaggttccc ccgtttctcc
     3721 atggaatgcc ctcctccccg gttcggacca tcgtttgcct gacctttgca ttgacgttat
     3781 catag
//
LOCUS       DAOOVN010000051         3673 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_13611_length_3673_cov_14.183527, whole genome shotgun
            sequence.
ACCESSION   DAOOVN010000051 DAOOVN010000000
VERSION     DAOOVN010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13611_length_3673_cov_14.183527"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      complement(67..157)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(282..1085)
                     /locus_tag="PLY83_04945"
     CDS             complement(282..1085)
                     /locus_tag="PLY83_04945"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOV29414.1"
                     /translation="MVLVKGLLFKLDDVLSSEARLTVGLYHRVAYRLSKRPGGPDIRA
                     VLLSLLISQEEGAADSRAWNRLYHAVPEIPRIIRYTDLLQLVKKTLPVGTLFQGFREL
                     AAVLQEIGVRTAVMAGPYCRLQQNKVSALRLEDLFGAVVYTDFFCRNPRQAVSGAIRK
                     LLTMWKGLRPSEVAYIADDPASDIAAARMAGLHALRLRLHPTRHEPDEPKSDLERAER
                     EFTSVRELIAALCRYYRIEPETVLPGEWDYYLKPQPWRLMPRLKRNRRI"
     gene            complement(1132..2319)
                     /locus_tag="PLY83_04950"
     CDS             complement(1132..2319)
                     /locus_tag="PLY83_04950"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOV29415.1"
                     /translation="MSASRISRNVWALSAANFLIAAFTKCYMLLPAYMAASGVSDPGR
                     IGWLMGSFYVSSFVRPFVGWLTDAAGFRRVLFFSGVLAAVSGLGILAAGPDSLVLLIG
                     CRILTGIAYSLFAVALTAYQSLVLAGEAQGAGLSVITSTTNLPFLLVVPVCEALLSAG
                     FLKTYLVVPVLLAAGTCAVSLRLPQADLLRPVRRGSAVTTASILNNPNLQMLFVSITL
                     FCSVDAGLLSLAGLGRERGLPVSSFFAASAVSSLLVRFFGRSLPDRLPRVRTSWICAA
                     AAAVILAACALPFIRSPLAFAACGGVYGIFIGLGYPILLALISDTAAPEQRSRVTSLF
                     WFFMGLAYLFMPVITGYLAALLRYKAAFFLLNLALVPLLAFAGLRWEKLQAPRMIPAE
                     KSH"
     gene            complement(2548..2808)
                     /locus_tag="PLY83_04955"
     CDS             complement(2548..2808)
                     /locus_tag="PLY83_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29416.1"
                     /translation="MRTITSRELKNNPALLASGEITLVTSRGKPVALCLPLREGDDPD
                     SLAAAILSLLGQLAQKEPPDKKEIRGEITAVRVARRKTGEQP"
     gene            complement(2857..3213)
                     /locus_tag="PLY83_04960"
     CDS             complement(2857..3213)
                     /locus_tag="PLY83_04960"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOV29417.1"
                     /translation="MRDPELLLEDILECTEKIQAYLQGVTKPSFMANGILQDAVVRNL
                     EIIGEAAKGLPASFQDQYPEIQWRKIIALRNILAHVYFGIDNEIIWDLATREIPLLKS
                     RIVAILDRKKPGPEEI"
     gene            complement(3206..3496)
                     /locus_tag="PLY83_04965"
     CDS             complement(3206..3496)
                     /locus_tag="PLY83_04965"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOV29418.1"
                     /translation="MKREVIIDRIREQEAELRRMGVARLCLFGSAARGQADAESDLDF
                     LVAFEGTADYDRYITLKFFLEDLLGKPVDLVMETALKPRIRSRVESEMIRVA"
BASE COUNT          757 a         1038 c         1181 g          697 t
ORIGIN      
        1 gtgccttggg aagggacatt ccagccggca cttttccggg ccggccggac ctgctgaaag
       61 gaatccttcg gcagtccggc gaccctggca aaaacccact cgctttaggc ccgtgacttt
      121 gcgtccccgc ctttcgacgg gtttgccctt ttcggctttt ttgaagctgt ggatattcta
      181 tgcagaaatc tttttcttgt aaatcaggga aaccctgatt tgtaattttt aataataatt
      241 gactttttgc gtaaacgaag attcttcttt ttttgatcaa cctatattct tcggtttcgt
      301 tttagccggg gcatgagcct ccagggctgc ggcttgaggt aatagtccca ctccccgggg
      361 agcaccgttt cgggttctat tcgatagtat cggcagaggg cggcgatcag ttcacgcacg
      421 ctggtgaatt ccctctccgc cctttccagg tcgcttttgg gctcgtccgg ttcgtgccgg
      481 gtggggtgaa ggcgcaggcg aagggcgtga agccccgcca tgcgggcagc ggcgatgtcc
      541 gaggcgggat catcggcgat gtaggcaact tccgagggcc tgagcccctt ccacatggtc
      601 aaaagcttcc ggatggcccc ggaaactgcc tggcgcgggt tccggcagaa gaagtcggtg
      661 tagaccacgg cgccaaacag gtcttccagg cgcagggccg agaccttgtt ctgctggagg
      721 cggcagtaag gccccgccat gacggcagtc cggacgccga tttcctgaag gacggcggcc
      781 agctcccgga acccctggaa gagggttccc acaggaaggg ttttcttcac cagctggagg
      841 aggtcggtgt agcggatgat gcggggaatt tccggaacgg catggtacag gcggttccat
      901 gcccgggaat ccgctgcccc ttcctcctgg ctgatcagga gagacagaag caccgcacga
      961 atgtcgggtc cgccggggcg ctttgaaagc ctgtacgcta cccggtgata aaggcccacg
     1021 gtgaggcggg cctcgctgga aagaacgtcg tcgagcttga acaacaatcc ctttaccaac
     1081 accattctcc cctttctcgg aaggtgggaa acggtgtcga ttatgctcca gttaatggga
     1141 tttttcagcc ggaatcatgc gaggggcctg cagtttttcc caccgcaggc ccgcgaaggc
     1201 caggagggga accagggcaa ggttcaggag gaagaaggcc gccttgtacc ggaggagggc
     1261 ggccaggtag ccggtgatca cgggcatgaa gagataggcg agtcccatga aaaaccagaa
     1321 gagggaagtg acccggcttc gctgttcggg tgccgcggtg tcggagatga gggccagcag
     1381 gatcggatag cccaggccga tgaagatgcc gtagaccccc ccgcaggcgg caaaggccag
     1441 ggggcttcgg atgaacggca gggcgcaggc ggccaggatg actgccgcgg cggcggcgca
     1501 gatccaggag gtgcggaccc ggggcaggcg atccggaaga gagcggccga aaaagcggac
     1561 cagcagcgag gacacggccg aggcggcaaa aaaggaggaa accgggagcc ccctctcccg
     1621 gcccagcccg gcgaggctga ggaggccggc atcgaccgag cagaagaggg tgatcgaaac
     1681 gaaaagcatc tgcaggttcg ggttgttgag gatcgaagcc gtggtgacgg ctgacccccg
     1741 gcggacgggg cgcaaaaggt ccgcctgcgg caggcggagg gaaacggcgc aggttccggc
     1801 ggccagcagg acgggaacca ccaggtacgt tttcaggaac ccggcggaaa gcagggcttc
     1861 gcaaacgggg accaccagca ggaagggcaa gttggtggtg gaggtgatga cggaaagccc
     1921 ggctccctgg gcttcccccg cgagaaccag ggactggtag gccgtcaggg ccacggcgaa
     1981 aaggctgtag gcaatgccgg tcagaatccg gcagccgatg agcagaacca gggagtccgg
     2041 gccggcagcg aggatgccga ggcccgaaac ggcggcaagg accccggaaa agaacagcac
     2101 gcgccggaaa ccggcggcat cggtgagcca ccccacaaag ggccggacaa acgagctgac
     2161 gtagaagctc cccatgagcc agccgatgcg ccccgggtcc gaaaccccgg aagccgccat
     2221 gtaagccgga agcagcatgt agcacttcgt gaacgcggcg atgagaaagt tcgccgcgct
     2281 gagggcccag acgttcctgc tgatcctcga tgcgctcatg ggtgtcattt tacgcggaaa
     2341 agaccacaaa gagcggtgag gcacaaaccc tcggtcaggg aaggatcgtg acgggggttc
     2401 cgatgtctca atcctggcca cgaggcggag tcggtgtttt ccaggcggat gcagccttcc
     2461 gtgacggccc tgccgacgga agcagggtcg tgggtgccag gcggaggaac cagggaaggc
     2521 tacggagagg cttcatgccc aggcgcgtca gggctgctcg ccggttttcc ggcgggcaac
     2581 cctgactgcc gtgatttcgc cccggatctc ctttttgtcg ggaggttcct tttgggccag
     2641 ctggcccagt aggctcagga tggccgccgc aagggaatcc ggatcgtctc cttccctaag
     2701 cggaaggcat agggcaacag gctttcctct tgaagtgacg agcgtaatct ccccggacgc
     2761 aagaagcgcc gggttgttct tcagctcccg gctcgtgatg gtcctcatgg gcttcccctt
     2821 tcggcttccc ctgcggtgcg aactataaca ggatcgttaa atttcctcag gtccgggctt
     2881 ttttcgatca aggattgcca cgattcggct tttcagcagg gggatctccc gagtggcaag
     2941 atcccagata atttcgttat caatgccaaa atacacatga gcaagaatgt tccggagggc
     3001 gatgattttg cgccattgga tttcagggta ctggtcctgg aaggaagccg gcagtccttt
     3061 cgcggcttct ccgatgattt ccaggttgcg aacgactgcg tcctgcagga tcccgtttgc
     3121 catgaacgac ggcttcgtca ctccttgaag gtaagcctgg attttctccg tgcattcgag
     3181 gatatcctcg agcaagagct cgggatcacg caacacggat catctcgctt tcgacgcggg
     3241 agcgaatcct cggtttcaac gccgtttcca tgaccaggtc gaccggcttt cccagaaggt
     3301 cttcgaggaa gaatttcagg gtgatatacc ggtcatagtc ggccgtgcct tcaaacgcaa
     3361 cgagaaagtc caaatcgctt tcggcgtcgg cctggccgcg ggcagcagaa ccaaaaagac
     3421 acagtcgggc aacgcccatt cggcgcagtt ccgcttcctg ttcccttatc cggtcaatga
     3481 tgacttcccg tttcatccca agcgcctctt ttcatttgca cttcgctaaa agttattata
     3541 gccgacaagc cggcagaaca ggaaaagcgg gcctcgcctg cgcggggccc gcctgcttgt
     3601 ttttcccgtt ttttccttgc cctggccgct attccgccac cgggctgtcg atcatcgggg
     3661 aaggggctcc tcc
//
LOCUS       DAOOVN010000052         3509 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_14455_length_3509_cov_15.523162, whole genome shotgun
            sequence.
ACCESSION   DAOOVN010000052 DAOOVN010000000
VERSION     DAOOVN010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14455_length_3509_cov_15.523162"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..775
                     /locus_tag="PLY83_04970"
     CDS             182..775
                     /locus_tag="PLY83_04970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01926.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HOV29419.1"
                     /translation="MSLEFDEQLSYLQKPKREEISDEHFAVFEKAVETMEKNWGFINN
                     LFKVLPLNAAQYIGFLDFKASLFNPKTCYLSDADKEMIGLVVSSTNGCSYCLTTHSDY
                     LRGICGDPAWVDQLTYNYRAAKLTEKQRALCDYAYFVTVYPREITKEQVDKLRAVGFN
                     DHEILEAAFVAGFFNYTNRWVSTIEPIANPGHYSHNR"
     gene            788..2731
                     /locus_tag="PLY83_04975"
     CDS             788..2731
                     /locus_tag="PLY83_04975"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOV29420.1"
                     /translation="MPYEKLFQKGRIGNLTIRNRAVMTPMATDFASPDGTASMRLIRY
                     YQERAKGGIGLIINEYTGVDHVTSIPALFQLRASSDVHVAGLEMLVDAVHAHGACIFA
                     QLHHAGSTSKPALTGQQALSASDVPIAPGAPIPRPMTIEEIREVQRKFVDAAIRCRKA
                     GYDGVELHGAHSYLIGQFFSPYYNKRSDEYGGSFENRMRFIAEIIDGIREKLGGRFPI
                     SVRISGDEMTPQVPGTLNLQDGLQIGLYLQDKGIDVLNVSNGSGLNANANCDPYSYKP
                     GWKKYVAKAFKQVLRIPVIATNTIKDPDFAEQLLEEGVCDFVGLGRSQFADPEFVNKA
                     KAGRSDEIRPCIGCMVCRERLLAKGMTVVCAVNPRMGREHLYPDYRKNGMNRKVCVVG
                     GGPGGLEAARVLAERGFQVTLLEKQPVLGGTLNLADKPPHKELVTKLTKAMVRQVEVL
                     GVDVRCEMEATPEMVKALAPEAVFLATGAAPIIPKLPGIELANVHLAEDVVAGKAKPS
                     GKVAVIGTGMTGLETAEMLGNMGCRLTLVEMLPEIGPGIFPVIRNDILGRIMAFEPEL
                     LPGHRLVEIVPGSIRMEKADGTQAEVQVDHVVLSLGTAPNPALAEAFETACGRVIPIG
                     DAAQAGRIYEATKDAFDKAYHFD"
     gene            2743..3489
                     /locus_tag="PLY83_04980"
     CDS             2743..3489
                     /locus_tag="PLY83_04980"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOV29421.1"
                     /translation="MADLTLWKVLAEWKTPKYKWVDLTHELSPETPHWYGFQPLGIRV
                     LFDFDQAPMKVFEYTLPGQYGTHVDVPSHFDANGRTQSEIRVDEFAYPLCVIDVSAKC
                     AADNDYALTIDDVLEWEETYGKIPEGAFVALRSDWYKRPAAEFDNKDQEGCAHYPGWD
                     LETVKWLCADRKIGAMGHETPDSDPACRQAQIGFAAEDYILKQDKIQIELLCNLDKLP
                     PVGAVIFCTFPKLKGGTGFPARCFAICPAE"
BASE COUNT          782 a         1039 c         1057 g          631 t
ORIGIN      
        1 actaacacag catttatcgc ccttgacaag gatttgcttc gttgaattga gaaggtgtat
       61 catcattgtc gtcaggtgaa ggattcaaaa aagcaaagtg ctggtgcacg tacaaagaga
      121 aagccctcgg ctgcgtcaag agaacgcccc tgggcaaaac cccttctaag gaggtatttt
      181 catgagcctg gagtttgacg agcagctttc ctatctgcag aaaccgaagc gggaagaaat
      241 ttcggacgag catttcgcgg tgttcgaaaa agcggtggaa accatggaga agaactgggg
      301 gttcatcaac aacctgttca aggtgcttcc cctcaacgcg gcccagtaca tcggctttct
      361 cgatttcaag gcctcgctgt tcaacccgaa aacgtgctac ctgagcgacg ccgacaagga
      421 gatgatcggc ctcgtcgtgt cctccaccaa cggctgcagc tactgcctga ccactcacag
      481 cgactacctc aggggcatat gcggggatcc cgcgtgggtg gaccagctta cctacaatta
      541 ccgggcagcc aaactgacgg agaagcagcg ggcgttgtgc gactacgcct attttgtcac
      601 cgtgtatccc cgggagatca cgaaggagca ggtggacaag ctccgggcgg tcggtttcaa
      661 cgatcacgag atcctggagg cggccttcgt cgccgggttc ttcaactaca ccaaccgctg
      721 ggtaagcacc atcgagccca tcgcaaaccc aggccactat agccacaatc gctgagaggc
      781 agaaacgatg ccctacgaaa aactcttcca gaaaggccgt atcgggaacc tgaccatccg
      841 gaacagggca gtcatgaccc ccatggccac cgactttgcc tcgccggacg gaacggcctc
      901 catgaggctg atccgttatt accaggaacg ggcgaaaggc ggaatcggcc tgatcatcaa
      961 cgaatacacg ggggtcgacc acgtaacgag catcccagcc cttttccagc tcagggcctc
     1021 ctcggatgtc cacgtggccg gcctggaaat gctcgtcgat gctgtgcacg cacacggagc
     1081 ttgcatcttt gcccagcttc accacgccgg cagcacgtcg aaaccggcct tgacagggca
     1141 gcaggccctt agcgccagcg atgtgccgat cgcacccggg gcgccgatcc ccaggcccat
     1201 gaccatcgaa gagatccgcg aggtccaacg gaagttcgtg gatgccgcca tccgctgtag
     1261 aaaagcgggg tacgacggag ttgaactgca cggggcccat tcgtacctca ttgggcagtt
     1321 tttcagcccc tactacaaca agaggtccga cgaatacggc ggcagtttcg agaaccggat
     1381 gcgtttcatt gccgagatca tcgacggaat ccgggaaaaa ctcggggggc ggttccccat
     1441 ctctgtccgc atcagcggcg acgagatgac tccccaggtt cccggcactc tgaacctcca
     1501 ggacggcctc cagataggcc tgtacctgca ggacaagggg atcgacgtcc tcaacgtcag
     1561 caacggcagc ggcctcaacg cgaacgcgaa ctgcgacccc tattcctaca agcccggatg
     1621 gaagaagtat gtcgcgaagg ctttcaagca ggtcctgagg ataccggtca tcgcgacgaa
     1681 cacgatcaag gatccggatt tcgcagaaca gctgctggag gaaggggtct gcgattttgt
     1741 cgggctcggc cgttcccagt tcgcggatcc ggagtttgtc aacaaggcga aggcaggccg
     1801 atcggacgag attcgcccgt gcatcggctg catggtctgc cgggaacgcc tcctggcgaa
     1861 agggatgacg gtggtttgcg ccgtcaaccc aaggatgggg cgcgaacacc tttatccgga
     1921 ttaccgtaaa aacggcatga accgcaaggt ctgcgtcgtg ggaggcggcc ccggaggcct
     1981 cgaagccgcc cgcgttctcg ccgagcgggg gttccaggtg acgctcctgg aaaaacagcc
     2041 ggtgctggga ggaaccctga acctcgcgga caagcccccc cacaaggaac tcgtcaccaa
     2101 gctcacaaag gccatggtca ggcaggtcga ggtcctgggt gtcgatgttc ggtgcgagat
     2161 ggaagcgact cccgaaatgg tcaaggccct tgcccccgag gcggtttttc tcgctaccgg
     2221 tgccgcgccg atcatcccga aattgccggg gatcgaactt gcaaacgtcc accttgcgga
     2281 agacgtcgtg gctgggaagg caaagccgag cggaaaagtg gccgtcatcg gcacggggat
     2341 gaccggtctc gagaccgcgg aaatgctggg caacatgggc tgcaggctga cgctcgtcga
     2401 gatgctgccg gagatcgggc ccgggatatt ccccgtgatt cgcaacgaca ttctaggcag
     2461 gatcatggcc ttcgaaccgg aactgcttcc cgggcacagg ctcgtggaga tcgttcccgg
     2521 atccatccgg atggagaagg cagacggaac gcaggcagag gtgcaagtcg accacgtggt
     2581 cctatccctg ggcacggctc cgaacccggc tctcgccgag gccttcgaga ctgcctgcgg
     2641 acgcgtgatc cccatagggg atgcggcaca ggccggacgc atctatgaag cgacaaagga
     2701 cgccttcgac aaggcgtatc acttcgatta ggaggtgtta ttatggcgga cttgacgctc
     2761 tggaaggttc tcgcggaatg gaagacgccc aagtacaagt gggtggacct gacccacgaa
     2821 ctttccccgg agacccccca ctggtacggc ttccagccac tcggtatcag ggtcctcttc
     2881 gatttcgacc aggccccgat gaaagttttc gagtacacgc ttcccggcca gtacggaacg
     2941 cacgtggacg tcccgtctca cttcgatgcg aacggcagaa ctcaaagcga gatccgggtg
     3001 gacgagtttg cctacccgct ctgtgtcatc gacgtgtccg ccaagtgtgc agcggacaac
     3061 gactacgccc tcacgatcga cgacgtgctc gagtgggagg aaacctacgg caaaatcccc
     3121 gaaggggcgt tcgtcgcttt gcgttcggac tggtacaagc gtccggcagc ggagttcgac
     3181 aacaaggacc aggaagggtg cgcccattac ccgggttggg atctcgaaac cgtgaagtgg
     3241 ctctgtgcgg atcggaagat cggggccatg gggcatgaaa cgcccgacag cgatccagcc
     3301 tgccgccagg cccagatcgg gttcgctgcc gaggactaca tcctgaagca ggacaagatt
     3361 cagatcgaac tcctgtgcaa cctcgacaag cttccgccgg tcggtgccgt gatcttctgc
     3421 accttcccga agcttaaggg cggcaccggt ttcccggctc gctgttttgc catctgtcct
     3481 gccgaatagg gcatggaggg gcgggcgga
//
LOCUS       DAOOVN010000053         3228 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_16074_length_3228_cov_14.935708, whole genome shotgun
            sequence.
ACCESSION   DAOOVN010000053 DAOOVN010000000
VERSION     DAOOVN010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16074_length_3228_cov_14.935708"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="PLY83_04985"
     CDS             complement(<1..570)
                     /locus_tag="PLY83_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical-resistance protein"
                     /protein_id="HOV29422.1"
                     /translation="MARAVPNGDRKLTNVFERYLTIWVALCIVGGILLGKIAPNLARA
                     LDGMAIRVNGAPVVSIPIAICLFFMMYPIMVKIDFAQVIRAGKSGKPVFLTLFVNWAI
                     KPFTMYAIALFFLGFLFKGFIGPDAVDLVKMPFGLNLPVGASHGDGIVVLVNGVKQLQ
                     VPLWRSYLAGAILLGIAPCTAMVLVWGYLA"
     gene            complement(687..947)
                     /locus_tag="PLY83_04990"
     CDS             complement(687..947)
                     /locus_tag="PLY83_04990"
                     /inference="COORDINATES: protein motif:HMM:NF016230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOV29423.1"
                     /translation="MTAPTDWKRAPSSQTVCYCNNVKKDEIVRAIANGAETVEDVTRL
                     TGAGKGTECSTKNPWGRCCCPDIQALIDATLPALKAIRSGCT"
     gene            complement(970..1296)
                     /locus_tag="PLY83_04995"
     CDS             complement(970..1296)
                     /locus_tag="PLY83_04995"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOV29424.1"
                     /translation="MKALSDRHRVRALLALEGQELCLCNLQDLLGLVPSSVSRHMSIL
                     QEAGFVVSRKKGKWTYFSLAPLDENTGLSGLLRWLLESLGDDPATLADRELVRQLKNK
                     DSERCK"
     gene            1544..>3228
                     /locus_tag="PLY83_05000"
     CDS             1544..>3228
                     /locus_tag="PLY83_05000"
                     /inference="COORDINATES: protein motif:HMM:NF012570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HOV29425.1"
                     /translation="MQDKETLLLAARKLEGMPLSAHQREFLAEIQERLNSDKLFVAVV
                     GEFKRGKSTLINAILGEPVLPTGVLPLTNIVTLIEHGPVPSLEVTFRDRPLLEAPIDR
                     LNEFVSEAGNPENRKKVRFVRLRYPSAFLETGIVLVDTPGFESLHETQTEEARSALPR
                     IDIGLFLLSADSPLSAKEMALLDELAGYVSHCLILLNKTDFLTEPERRAMVEYIRSRI
                     SERHPGMAEAVFPVSARESLLASGGKKTGSRDVGGIGAVMDRLEALVREKGHGIQVKS
                     NAGRLLSIARAVKARLQVEADSLRQPIRVLEDRIARFSSGLERLETRRSEAEILVDSQ
                     IERIIQELDAEVDRLRRELPPLLCADLREGSEALAGESVPEFSARMAALAGDRLKARF
                     EERLPAIEAKIESAYLEVVEKHRSRLTDILDELLRFGSEVFSLDLQSWELPEEFSSER
                     RFFYLEGETRPFFDLEAAAIGFMDRLLPAGKARERALARILERVPERVDANCGRVRFD
                     LLRRLKDSAARFKGRLARLVTESAQGIQEAAKGALEARSKAQEEGAEREKLLET"
BASE COUNT          601 a          993 c         1045 g          589 t
ORIGIN      
        1 ggccaggtag ccccacacca gcaccatggc ggtgcacgga gcgatcccga gcagaatggc
       61 cccggccagg tagcttcgcc agaggggcac ctgcagctgc ttgacgccgt taacaaggac
      121 cacgatgccg tctccgtgcg atgctcccac cggcaggttc aggccgaagg gcatcttgac
      181 gaggtccacc gcgtctggac cgatgaaccc cttgaagagg aatcccagga aaaagagcgc
      241 gatggcgtac atcgtgaagg gcttgatggc ccagttaacg aacagggtca ggaaaacggg
      301 ctttcctgac ttgccggccc ggatcacctg ggcgaagtcg atcttcacca tgatggggta
      361 catcatgaag aacaggcaga tggcgatggg gatcgatacg acgggggccc cgttgacccg
      421 gatcgccatc ccgtccaggg ccctggcaag gttgggagcg attttcccga gcaggatccc
      481 tcccacgatg cagagggcca cccagatcgt caggtagcgt tcgaaaacgt ttgtgagctt
      541 gcggtcaccg tttgggactg cccgtgccat agagaaattc cctccttgtc gggagcgtga
      601 aggttcgggt tcaaaagcgg ttgattccgt ccagcaagga tcattcggga ttgcggcgga
      661 ccgccgcgtt ttcagcagtt gccccctcag gtgcacccgc tcctgatggc cttgagcgcg
      721 ggaagagtcg cgtcgatcag ggcctggatg tccggacagc agcagcgtcc ccaggggttc
      781 ttcgtcgagc actcggttcc ctttccggcg cctgtcagcc tcgtgacgtc ttccacggtt
      841 tccgccccgt tggcaatcgc ccggacgatc tcatcctttt tcacgttgtt gcagtagcag
      901 acggtctggc ttgatggcgc ccttttccag tcggttggtg cggtcatcgt ttcctcccct
      961 tccgtggctt tacttgcagc gttcgctgtc tttgttcttc agttgccgga cgagctcccg
     1021 gtcggccagg gttgccgggt cgtctcccag gctttcgagg agccagcgga gaaggccgga
     1081 gaggcccgtg ttttcgtcga gcggtgccag gctgaagtag gtccacttgc ctttcttcct
     1141 tgaaacgacg aagcctgcct cctgcaggat cgacatgtgc cgggaaacgg aggagggaac
     1201 gagtcccagg aggtcctgga ggttgcaaag gcaaagctcc tgtccttcca gggccaggag
     1261 cgcccggacc cggtgccggt cagatagagc cttcatcgcg ttcaggatgt cttccaggga
     1321 aaggccctcc cctccaaatt catttcgaac attttcgaaa tgaaagatac ctcaaccggg
     1381 ggaaggctgt caaccggacc ggcaaggacg cttgaagggg ataaaccccc gtgttacgat
     1441 ttcgctcggc gatggcgttc gccctttctg ctccgccctt gacgggccgg atgatgacgc
     1501 ctgtgattcg gtcccatcgc gggccccatc ggaggcgaaa cgaatgcagg acaaggaaac
     1561 tctcctctta gcagcccgaa agctcgaagg aatgccccta tcggcccacc agagggaatt
     1621 cctggccgaa atccaggagc gcctcaactc ggacaaactc ttcgtcgcgg tggtgggcga
     1681 gttcaagcgg gggaaatcga ccctcatcaa cgcgattctc ggagaaccgg ttttgcccac
     1741 cggcgtgctc cccctcacca acatcgtgac cctcatcgag cacgggcccg ttccttccct
     1801 ggaagtgacg ttccgggacc ggcccctgct ggaggccccc atcgaccggc tcaacgagtt
     1861 cgtctcggag gcgggcaacc ccgaaaaccg caagaaagtc cgcttcgtcc ggctccgtta
     1921 cccttccgct ttcctggaaa ccggcatcgt cctggtcgac acgccggggt tcgagagcct
     1981 ccacgagact caaaccgagg aggcccgctc cgcccttccc cggatcgaca tcgggctgtt
     2041 cctgctttcg gcggactccc ccctgtcggc gaaagagatg gccctcctgg acgagctggc
     2101 cgggtacgtt tcccactgtc tcatcctgtt gaacaagacg gatttcctga ccgagccgga
     2161 gcgcagggcc atggtggagt acatccgaag ccggatctcc gagcggcacc ccgggatggc
     2221 ggaagccgtg ttccccgtgt cggcccggga gagcctgctc gcctcgggcg ggaaaaagac
     2281 cgggtcccgg gatgtcggcg gaatcggtgc cgtcatggac cgcctcgaag cgctggtccg
     2341 ggagaaggga cacgggatcc aggtcaaaag caacgcgggg agacttttat ccatcgcccg
     2401 cgcggtcaag gcccggctcc aggtggaggc cgattcgctc cggcagccga tccgggtcct
     2461 ggaggaccgg atcgcccgtt tctccagcgg gctcgagagg ctggaaaccc ggcggagcga
     2521 ggcggagatc ctggtggata gccagatcga gaggatcatc caggaactgg atgcggaagt
     2581 ggaccgcctg cggcgggaac tccccccgct tctctgtgca gacctccggg agggatccga
     2641 agccctcgcg ggagagtccg ttccggaatt ttcggcccgg atggcggcct tggcggggga
     2701 ccggctgaaa gcgcgtttcg aggagcgtct cccggccatc gaggcaaaga tagagtcggc
     2761 ctacctggaa gtcgtggaaa agcaccgctc ccgcctgacg gacatcctgg acgagctgct
     2821 gcggttcggc tcggaggtgt tcagcctgga cctgcagtcc tgggagctgc cggaagagtt
     2881 ttcctccgag cggcgtttct tctacctgga aggagagacg cggccgtttt tcgacctgga
     2941 ggcggccgcg atcgggttca tggaccggct gcttcccgcc ggcaaggccc gggaaagggc
     3001 cctcgcccgg atcctcgaga gggtcccgga gagggtcgac gccaactgcg gacgtgtccg
     3061 cttcgatctt ttgaggcggc tgaaggacag cgccgcccgc ttcaaggggc gcctcgcgag
     3121 actggtgacc gaatcggcgc aggggatcca ggaggcggca aaaggagcgc tcgaggcgcg
     3181 gtcaaaggcc caggaggaag gggcagaacg agagaaactc ctggaaac
//
LOCUS       DAOOVN010000054         3222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_16119_length_3222_cov_13.507105, whole genome shotgun
            sequence.
ACCESSION   DAOOVN010000054 DAOOVN010000000
VERSION     DAOOVN010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16119_length_3222_cov_13.507105"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="PLY83_05005"
     CDS             <1..921
                     /locus_tag="PLY83_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOV29426.1"
                     /translation="DRGIILVTDQCSMYCRHCTRRRMAGETDRPFSRAQMEACFESIR
                     NNPGFRDLLFTGGDPFTVDDETIEWLLEEAWSIPHLEIVRFGTRTPVVMPQRITDNLC
                     RILEKYPPVWINTHFNHPNEITPESKAACDRLVRAGVPLGNQSVLLKGVNDCPYIFRE
                     LNQKLLTIRVRPYYIYQCDLSPGIEHFRTAVAKGIQIMEYLRGHTSGLAVPYYIIDSP
                     GGGGKIPILPNYLVSMSDRKVILRNYEGTFTTYTEPEDKNSRCPEHCGRICERQKAFS
                     REGLIPLFEGDRVSLQPETTARKRRAEGKA"
     gene            complement(976..2634)
                     /locus_tag="PLY83_05010"
     CDS             complement(976..2634)
                     /locus_tag="PLY83_05010"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOV29427.1"
                     /translation="MAMTWTIENTPEGLFRLLLDLVAVRSISLTPGEVEIAEKIEESL
                     RALPYFRSNPDDLRFIPIPGDALGRKSVMALVRAQPATRKTIVVIGHADVVGAEPYGS
                     LADLAFDPLALSAYLRNEDLSPEVRADLESGDFLFGRGVGDMKAGVAIGMALVAERSA
                     DPSSLGANLLFLALVDEEISSAGMRAAVPVLAELQEELDFVACLNTEPSDPGFSQGRN
                     HCFSLGTAGKHNLFFLFAGRASHSTAYFEGFNAILPAAHLAVLVEGNDSLTDRSPRDT
                     FSPMGCLKLKDLRKTYSGTLPETAAAYVNLNPVHRMPGEILVSMERFAWEALDRSLRQ
                     VKEAYLRYGEHIGQWPPRGEWHGKVLSYAELAEAAQKHAGVGYEETLRKFIAGLPAGM
                     EEQDRCIAVAEETLRLSGMKGPLVIYGFLPPYYPQRVTTGRTPRERALVEAVEWAVSE
                     GASRFGVKIEVNDYFGGVSDLSFFGYPGKASDLEALSRNLPGWGTVFPFPEKDLPRLD
                     VPVANIAPAGKDSHKKTERLEVPYSLKVVPRLLGGIFDRLAEGE"
     gene            complement(2932..>3222)
                     /locus_tag="PLY83_05015"
     CDS             complement(2932..>3222)
                     /locus_tag="PLY83_05015"
                     /inference="COORDINATES: protein motif:HMM:NF016999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HOV29428.1"
                     /translation="SATPEVIGTLQRMLLPTLAITAAMVAWGIVLAFVVRRITRWSLM
                     TCLIATCPGGITQLASIAEELGADPLRVSLLHLVRLFTIFLVLPPLISRILA"
BASE COUNT          636 a         1013 c          990 g          583 t
ORIGIN      
        1 gaccggggca ttatcctggt gaccgaccag tgttccatgt actgccgcca ctgcacgaga
       61 agaaggatgg ccggggaaac cgaccgcccc ttctcccggg cccagatgga ggcctgtttc
      121 gagtccatcc gaaacaaccc tggcttccgg gatctgcttt tcacgggagg ggaccccttc
      181 acggtcgatg acgaaacgat cgagtggctc ctggaagagg cctggagcat cccccacctg
      241 gagatcgtcc gcttcggcac ccgaacgccg gtcgtcatgc cccagcgcat cacggacaac
      301 ctctgccgca tcctggaaaa atacccgcct gtctggatca acacccactt caaccacccg
      361 aacgaaatca ccccggagtc gaaagccgcc tgcgaccggc tggtgcgggc cggcgttccc
      421 ctgggaaacc agtcggtcct cctgaaaggc gtgaacgact gcccgtacat cttccgcgag
      481 ctgaaccaga aactgttgac gatccgggtg cgcccctact acatctacca gtgcgacctc
      541 tcgccgggca tcgagcactt ccggaccgcg gtggccaagg ggatccagat catggaatac
      601 ctgcggggac acacgtccgg cctggcggtt ccgtattaca tcatcgacag ccccggaggc
      661 gggggcaaaa tcccgatcct gcccaactac ctcgtatcca tgtcggaccg taaggtcatc
      721 ctgcggaact acgaggggac cttcaccacc tacacggaac ccgaggacaa gaacagccgc
      781 tgccccgagc actgcggaag gatttgcgaa aggcagaaag ccttttcccg ggaagggttg
      841 atcccgctgt tcgagggaga ccgcgtctct ctccagccgg aaacgaccgc caggaaaagg
      901 cgggccgaag ggaaagcctg atttttgggt gaaaaggggc ggacctcggt ccgcccccct
      961 tcaatccagt cggctctatt ccccttccgc cagacggtcg aaaatccccc ccaaaaggcg
     1021 gggaacgacc ttcagcgaat agggcacttc cagccgttcc gtctttttgt ggctgtcctt
     1081 tccagccggg gcgatgttcg ccacaggcac gtccagtctg ggcaggtcct tttccgggaa
     1141 agggaaaacc gttccccagc cggggaggtt tcttgaaagc gcttccaagt cagacgcttt
     1201 tcccgggtat ccgaaaaagc taaggtctga gaccccgccg aaatagtcgt tcacttcgat
     1261 tttgaccccg aagcgggaag ctccttcgga caccgcccat tccacggctt ccaccagcgc
     1321 cctttccctt ggggtccgcc ccgtggtcac gcgctgggga taatacggcg gaagaaagcc
     1381 gtagatgacc aggggacctt tcatcccgga caaccggagg gtttcctctg ccacggcgat
     1441 acagcggtcc tgctcttcca tcccggccgg caggccggcg atgaatttcc gaagggtctc
     1501 ttcatagcct acgcccgcgt gcttttgcgc agcctcggcc agttccgcgt aggaaaggac
     1561 cttgccgtgc cactctcccc ttggcggcca ttgcccgatg tgctccccgt agcggaggta
     1621 ggcctccttg acctggcgga gggaccgatc gagggcctcc caggcaaacc tttccatgga
     1681 aacgaggatc tccccgggca tccggtggac ggggttgagg ttcacgtagg cagcggccgt
     1741 ctcggggagg gtccccgagt aggttttcct caggtccttc agcttcaggc agcccatggg
     1801 agaaaaggtg tcccgcggag agcggtcggt cagggaatcg ttcccttcca ccaggacggc
     1861 gaggtgcgcg gccggcagga tggcgttgaa gccctcgaaa taggcagtgc tgtgggaggc
     1921 ccggcccgcg aacaggaaaa acaggttgtg ctttccggcg gtgcccaggg agaaacaatg
     1981 gttccgcccc tgggaaaaac cggggtcgga aggctccgtg ttcaggcagg cgacgaaatc
     2041 gagttcttcc tgcagttcgg ccagcacggg gacggcagcc cgcatcccgg ccgagctgat
     2101 ttcctcgtcg accagcgcaa ggaaaagcag gttcgcgccg agggaggaag gatctgccga
     2161 tcgttccgcc accaatgcca ttccgatggc caccccggct ttcatgtcgc cgacgccgcg
     2221 gccgaaaagg aagtccccgc tttccaggtc tgcccgaact tccggggaca ggtcttcgtt
     2281 tctcaggtag gccgacaggg cgagcgggtc gaacgccagg tcagccaggg atccgtaggg
     2341 ctccgcgccg accacgtcgg catggccgat cacgacgatc gtctttcgcg ttgccggttg
     2401 ggcccggaca agggccatca cgctttttcg gcccagggca tcgcctggaa tcgggatgaa
     2461 gcgaaggtcg tcgggattcg aacggaagta ggggagggcg cgaagggatt cctcgatctt
     2521 ttctgcgatt tcgacctctc cgggggtcag ggaaatgctt ctgaccgcca ccaggtcgag
     2581 aagaaggcga aacaggcctt cgggagtgtt ttcaatcgtc caggtcattg ccatccgccc
     2641 ctccgggtca ccgggaaaag aggacgggaa cttgaatcca tggcttgaac gggaacggca
     2701 gactcttcca ggggaaagga tgtacgcaga tcacgcatgg gattcacctc tttttgccgg
     2761 caaggtcagc ctgctttccc ggtctcttga gagaaagcgc gcctccggcc agaatcggtt
     2821 tccccccggg tcaggcccgc ttctttcccc gcggccccgg gaacgctcct ccttttcctt
     2881 gccttggaaa cggtccttcc cattctagcg caacacggac gccccttgag cctaagccag
     2941 gatccgggag atcagggggg gcaggaccag gaaaatcgtg aaaaggcgca ccaagtgcag
     3001 caggcttacc cgcagggggt cggctcccag ttcctcggcg atagaggcca gctgagtgat
     3061 gcctccgggg caggtggcga tgaggcaggt catgaggctc cagcgggtga ttcttctcac
     3121 cacgaaagcc aggacgattc cccaggccac catggcggcc gttattgcaa gggttggcag
     3181 gagcatgcgc tggagggtcc cgatgacttc cggagtggca ct
//
LOCUS       DAOOVN010000055         3004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_17717_length_3004_cov_12.146151, whole genome shotgun
            sequence.
ACCESSION   DAOOVN010000055 DAOOVN010000000
VERSION     DAOOVN010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17717_length_3004_cov_12.146151"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1267
                     /locus_tag="PLY83_05020"
     CDS             <1..1267
                     /locus_tag="PLY83_05020"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOV29429.1"
                     /translation="VPKDGSVQGRAYRTRNSQVVDDVLRDPEYFPGIKGAGAQMAVPI
                     LWKNRAYGVLDIQSRKVRSLGNRQLRTAELLAGILGEAFARMQEAARLSLELTRTRIL
                     HDVVQELARSRDKRDMCQKVLGLLSTKLLYPVASILAVESENPLRLGYLASTRLSPSE
                     LKRYSRQLNREGGGLVTLSASMRKLHNVPDVRAFPEYRKVGFGVEGSQLDVPILFGDR
                     LYAMLSLERDVPFEPEDEDLMLILSRHMAVLWALFEAMEKLETQALQDSLTGLGNRRA
                     LEDILSLEEARLARFGGTIAVVMADLANFKAINDRYGHLVGDRYLKETAECIQKNLRA
                     CDHAFRYGGDEFLLLLPGTDRKGVQEVMNRVRNECRLRGNEDPDGILLDFGAAVCPTE
                     TVSLRAALRLADDRMYQNKEARRSGLSRD"
     gene            1570..2520
                     /locus_tag="PLY83_05025"
     CDS             1570..2520
                     /locus_tag="PLY83_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOV29430.1"
                     /translation="MLQIVKSENNTLRELDPASFEKGCWINLMDPSSEELNTVKKLVG
                     LPLDILTAALDEEESSRLEIEDEYLLVIIDVPRKRDNDRYETLPMGIILLPDVIVTVC
                     LEESDVLTCFNRMTARLFDTGKRTSFLFNILYRSATYFLRYLRQINIRTDEIERHLRQ
                     SMKNEELFQLLEMEKSLTYFSAALRTNNMVVDKILRLRNNRKVEHLIKIYEEDEDLLE
                     DALVEYRQAIEMVQMYSEILSGMMDAFASVISNNLNMVMKFLTSMTIILAIPTMFASF
                     WGMNVGVPWLDKAAGFWAVVGISLVATIGSAYALWRRNML"
     gene            2658..>3004
                     /locus_tag="PLY83_05030"
     CDS             2658..>3004
                     /locus_tag="PLY83_05030"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOV29431.1"
                     /translation="MSGKPPAWKIVAAFAAIYVIWGTTYLAIAMMVKSIPPYLGAGSR
                     FLVAGTLLFAFLRLRGQPLPTPRQWGGAALAGLLLLVGGNGSIVWAQKTVPSGIASII
                     VATEPLWIVLASWL"
BASE COUNT          591 a          932 c          894 g          587 t
ORIGIN      
        1 ggtcccaaag gatggaagcg tccagggaag agcctatcgg acccgcaatt cgcaggtcgt
       61 cgacgacgtc ctgcgggatc ccgaatattt tcccggcatc aagggggcgg gggcacagat
      121 ggcggttccc attctctgga aaaaccgggc atacggggtc ctggacatcc agtcccggaa
      181 agtccggagc ctgggaaacc gccagttgag aaccgcggag ctgctggcgg gaatcctcgg
      241 cgaagcgttt gcccggatgc aggaagcggc acgcctttcg ctggagctga cccggacccg
      301 catcctgcac gacgtggtcc aggaactggc ccgttcccgg gataagcggg acatgtgcca
      361 gaaagtcctg gggctgcttt ccaccaagct gctctatccg gtcgccagca tcctggcggt
      421 ggagtcggaa aaccccctgc gcctgggcta cctggcctcc acccggcttt ccccctcgga
      481 gctgaagcgg tacagccgac agttgaaccg cgagggagga gggctggtga cgctttccgc
      541 aagcatgaga aagcttcaca acgtccctga cgtgagggcc ttcccggaat accggaaagt
      601 gggcttcggc gtggagggaa gccagctgga cgtgccgatc ctcttcgggg accgcctcta
      661 cgcgatgctt tccctcgagc gggatgtccc gtttgaaccc gaggacgagg acctgatgct
      721 catcctttcc cgccacatgg ccgtcctctg ggctcttttc gaagccatgg aaaaactcga
      781 gacccaggcg cttcaggatt ccctcaccgg tttgggaaac aggcgcgccc tggaggacat
      841 cctcagcctg gaggaggccc gcctggcgcg gttcggggga accattgccg tcgtcatggc
      901 tgacctcgcc aatttcaagg ccatcaacga ccggtacggg cacctcgtgg gagaccggta
      961 cctgaaggaa accgcggaat gcatccagaa aaacctccgt gcctgcgacc acgcctttcg
     1021 atacggcggg gacgagttcc tcctcctgct tcccgggacc gaccgcaaag gcgtccagga
     1081 agtgatgaac cgcgtgagaa acgagtgccg gctcaggggg aacgaggacc ccgacgggat
     1141 cctgctggat ttcggcgcgg ccgtctgccc gacggaaacg gtaagcctta gggcggccct
     1201 gcgcctggcg gacgaccgga tgtaccagaa caaggaagca agaaggtcgg ggctttcccg
     1261 ggattgactg tcttcccctt tcggggcgct tgatggtata ctccattcca gactggccaa
     1321 ggatggtggt tttcccgtga gtacgggtga ttcgccgtta agtagacacg ataggggcgt
     1381 cgcggcgtag ctttccgaac agacgggaaa aacgcgctcg gcgcccgccg ggcgtggatt
     1441 attgcctttc cgatccactc cctttttcga tcaccttttt gaacgatcat tcaaacggac
     1501 agagaagaag cggcaggaca ccggatcttg cccgtttctt tctgcccgcg tggaagggat
     1561 ggtgaggaca tgctccagat cgtcaagagc gagaacaaca ctctccgcga actcgatccg
     1621 gcttctttcg aaaaggggtg ctggatcaac ctgatggacc ccagttccga agaactgaac
     1681 acggtgaaga agctcgtcgg cctgccgctg gacatcctga ccgcggccct ggacgaggag
     1741 gaaagctcgc gcctcgagat cgaggacgag tacctccttg tcatcatcga cgttccccga
     1801 aagagagaca acgaccgcta cgagaccctg cccatgggca tcattctcct tcccgacgtc
     1861 attgtgaccg tttgcctgga agagagcgac gttctgacct gcttcaaccg gatgaccgcc
     1921 cggctgtttg atacagggaa acggacgagt ttcctgttca acatccttta ccgctccgcc
     1981 acgtacttcc ttcgctacct tcggcagatc aacatccgga cggacgagat cgaacggcac
     2041 ctgcggcagt cgatgaaaaa cgaggaactt ttccagctcc tggagatgga aaagagcctg
     2101 acctatttct ccgcggcctt gagaaccaac aacatggtgg tcgacaagat cctccgcttg
     2161 cggaacaacc gcaaggtgga gcacctgatc aagatctacg aagaggacga ggacctgctc
     2221 gaggacgccc tggtcgaata ccggcaggcc atcgagatgg tccagatgta cagcgaaatc
     2281 ctgtcgggaa tgatggacgc ctttgcctcc gtcatctcca acaacctgaa catggtcatg
     2341 aaatttttga cctccatgac gatcatcctg gccattccca ccatgtttgc cagcttctgg
     2401 ggcatgaacg tcggggtccc ctggctcgac aaggccgccg gtttctgggc cgtggtgggg
     2461 atctccctcg ttgcgacgat cgggtccgcc tacgccctct ggcgccgcaa catgctctga
     2521 ttcgggggga ggacctccgt ttcccgctcg gcttgcagac ggggcgccac cgctgaacgg
     2581 cggaagcgcc cctccttttt tccccgtttt cggttatgat tcattaaagc ccatccccga
     2641 aaggaaggtt caccccaatg agcgggaaac ctccggcctg gaagatcgtc gccgccttcg
     2701 cggccatcta cgtcatctgg ggaaccacct acctcgccat cgcaatgatg gtgaagtcga
     2761 tcccccccta cctcggggcc ggttcccggt ttcttgtggc cgggactctc cttttcgcct
     2821 ttctccgcct tcgcgggcag ccgcttccga ccccgcggca gtggggcggg gcggcccttg
     2881 ccggccttct tcttcttgtg gggggcaacg gcagcatcgt ctgggcccag aagacggttc
     2941 cttccggaat cgcctcgatc atcgtcgcta ccgagcccct ctggattgtc cttgccagct
     3001 ggct
//
LOCUS       DAOOVN010000056         2791 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_19533_length_2791_cov_12.437500, whole genome shotgun
            sequence.
ACCESSION   DAOOVN010000056 DAOOVN010000000
VERSION     DAOOVN010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19533_length_2791_cov_12.437500"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..74)
                     /locus_tag="PLY83_05035"
     tRNA            complement(3..74)
                     /locus_tag="PLY83_05035"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40..42),aa:Val,seq:tac)
     gene            complement(113..1234)
                     /gene="metX"
                     /locus_tag="PLY83_05040"
     CDS             complement(113..1234)
                     /gene="metX"
                     /locus_tag="PLY83_05040"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HOV29432.1"
                     /translation="MAESTAEIIGSVNIPSLELESGRILRNVRQVYSRYGTPSPDGSN
                     VVVVCHALTGSHRVSGGARPGLPQPWWEPVVGHHKAIDTSSLCVYCFNILGSPYGSTS
                     PISRNPDTGRPWGIDFPLFSVRDIVKAEKTALELLGFPKIAAIVGGSLGGMQAIEWGI
                     LYPETVDRIVVIGAPAHLYPQAIAFNEVQRQAIFADPNWNNGRYHGGPPPSKGLAMAR
                     MLAMITYKSENCFAQRWMRQVKNGDLSDWSGQFQVESYLHHHGEELVRRFDASCYVYL
                     TRAMDRHDIGAGRGGALEALKRIERKHLLTVGISSDMLFPTWQVEELAELAQEAGVNA
                     FYDEIESDEGHDAFLIDFDQVDDSLRGFWERSGLTVRMC"
     gene            complement(1215..2546)
                     /locus_tag="PLY83_05045"
     CDS             complement(1215..2546)
                     /locus_tag="PLY83_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HOV29433.1"
                     /translation="MAYKQGEKTGLMTRALHAGWSHDPATGSFGLPVYMTAGYRFESP
                     EMAMRLFELEEPGHLYSRISNPTVTAFEQGLAALEGGVDCVATASGQAAFSHLLSALC
                     STGDHVVAGRRLYGGTLTLLKNLFGRLGVTSTIVETDDLDAVRAAVTPRTRCILTETI
                     GNPVMNVAPLEGLSKIARENGIPLVVDNTFASPALCRPFEWGASIVIHSATKYISGHG
                     HVIAGAIVDGGNMDWKGSGKWGFLTQPDPSYHGLVFADRFGPGALAAKIRCSILRDLG
                     GCLSPFNAYLLHLGLATLPLRMARHSENALKVASFLEGHPGVEWVRYPGLSSHPQKSL
                     ADRYLPNGAGGMLAFCIKGGVEAGKAFLKSLQLFATVANLGDSRSMALHPASTTHGQL
                     SPADRKAAGIEEGLIRLSVGIEDIDDILADLDQALENAGRAGGSCHGRVDG"
     regulatory      complement(2610..2713)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
BASE COUNT          564 a          858 c          798 g          571 t
ORIGIN      
        1 ggtgggcgaa acagggttcg aacctgtgac cccctgcttg taaggcaggt gctcttccgc
       61 tgagctatcc gcccgaacgc gagggatagt atatccggcc ttgaaaaacc tgtcaacaca
      121 tccttacggt caatccgcta cgttcccaga aaccccgcaa actgtcatcg acctggtcaa
      181 aatcgatcaa gaaggcgtca tgcccctcgt cgctttcgat ttcatcgtag aaagcattga
      241 caccggcctc ttgggcgagt tccgccagtt cttccacctg ccacgtgggg aaaagcatat
      301 cgctggagat ccccacagtc aaaagatgct tccgctcaat ccttttcagg gcctccagcg
      361 cccctcccct tcccgcgccg atgtcatgcc tgtccatggc ccgggtcagg taaacgtagc
      421 agctggcgtc aaaacgccgg accaattcct ctccgtgatg atgcaggtac gattcgacct
      481 ggaactgtcc ggaccagtcg ctcaggtcgc catttttgac ctgtctcatc catctttgag
      541 caaagcagtt ctcgctcttg taggtgatca tggccagcat ccgggccatc gccagccctt
      601 tcgagggcgg aggcccgccg tgataccgcc cgttgttcca gttagggtca gcaaaaatgg
      661 cctgccgctg gacctcgttg aatgcgatcg cctgcgggta aaggtgcgcc ggggctccaa
      721 tcacgacgat ccggtccacg gtttccgggt acaggatccc ccactcgatg gcctgcatgc
      781 cccccagcga ccccccgacg atcgcggcta tttttggaaa accgagcagt tccagcgcgg
      841 ttttctcggc tttcacgatg tccctcacgg aaaagagggg gaaatcgatt ccccagggac
      901 gtcccgtgtc gggatttctg gaaatcgggg aggtcgatcc gtaggggctc cccaggatat
      961 tgaagcaata gacgcacagg gaacttgtgt cgatggcctt atgatgaccg acgaccggtt
     1021 cccaccaggg ctggggcaat ccaggccgcg cccctcccga aaccctgtgg cttcccgtaa
     1081 gggcatgaca gacaaccacg acgttgctcc catccggaga aggggtcccg tagcgggaat
     1141 agacctgccg gacgtttcga aggatccggc ccgattccag ttccagggaa gggatattga
     1201 ccgaaccgat gatttcagcc gtcgactctg ccatggcagg atccccccgc tcttcccgcg
     1261 ttttcgaggg cctgatccag gtccgcaagg atgtcatcga tgtcttcgat gcccacgctg
     1321 agccggatta atccttcctc gatcccggca gctttccggt cggcgggtga aagctgcccg
     1381 tgcgttgtgc ttgccgggtg cagtgccatg ctccgggaat ctccgagatt cgccacggtg
     1441 gcgaaaagtt gcagggattt caggaaagcc tttcccgcct cgactcctcc cttgatgcag
     1501 aaggcgagca ttccgccggc gccgttcgga agataccggt ccgcaagaga tttctggggg
     1561 tgactcgaca ggcctggata gcggacccat tcgaccccgg gatgtccttc aaggaaggag
     1621 gcgaccttca gggcgttctc gctgtgccgg gccatgcgca ggggaagagt ggcaagtccg
     1681 aggtgcagaa gataggcgtt gaacggggaa aggcaccccc cgagatcccg aaggatcgaa
     1741 caccggatct tcgccgcaag agcccccggc ccgaagcggt ccgcaaagac aagcccgtgg
     1801 taagagggat cgggctgggt caggaagccc cacttcccgg agcccttcca gtccatattt
     1861 cccccgtcca cgatcgcccc ggcgatcacg tggccatgcc cggatatgta cttcgtggcg
     1921 gaatggatga cgatggaggc accccattca aagggacggc acagcgccgg agaggcgaac
     1981 gtgttgtcca caaccagcgg gattccattt tccctggcga tctttgagag cccttctaaa
     2041 ggggccacgt tcatcacggg gttcccgatc gtttccgtga ggatgcaccg ggttctcggg
     2101 gtcaccgctg ctcggaccgc atccaggtcg tcggtttcca cgatcgtgct ggttactccc
     2161 agcctgccga acaggttttt cagcagggtg agggtaccgc catacagtct acggcccgcg
     2221 acaacatggt ctccagtcga gcagagcgca gaaagaaggt gggaaaaggc cgcctggccg
     2281 ctggcagtcg ccacgcagtc cactcctcct tccagcgcgg caagcccctg ttcaaaggct
     2341 gtcacggtcg ggttggaaat cctggaatag agatgaccgg gttcttccag ttcgaagagg
     2401 cgcatggcca tttcgggaga ctcgaaccga taccccgccg tcatatagac gggaagcccg
     2461 aaggaaccgg ttgcaggatc gtggctccag cctgcatgca gagccctggt catcaacccc
     2521 gttttttctc cctgtttgta agccattgac ctacacctcc cataaaatca gccccttcct
     2581 caagaggaag gggctaaaaa agtcttcctc tcctatcaag cagcgttccg ctgccggtct
     2641 tggcaccaca cctcacattc aggcaggttg ccgggcttca tcgggccgtt ccctccgcca
     2701 ctctggataa gagtctgtct gtttcggttg tctcggttca ggataaattg gatgagattc
     2761 taagatgcag ggcttgccat tgtcaagggc a
//
LOCUS       DAOOVN010000057         2628 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_21218_length_2628_cov_13.274777, whole genome shotgun
            sequence.
ACCESSION   DAOOVN010000057 DAOOVN010000000
VERSION     DAOOVN010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21218_length_2628_cov_13.274777"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="PLY83_05050"
     CDS             <1..1080
                     /locus_tag="PLY83_05050"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOV29434.1"
                     /translation="LGTLGEWSAKLSRKLGIRQKELPGQVDRPLRFLKYAILAAIIGF
                     TWKLGTLAWRAYDPWVAWMHLSGGLDAMAEAPWAFAVLFVTVFGASVFIERFWCRYLC
                     PLGALLAPLQKLSFFKVRRSEEHCIHCHLCAQACPVRLDPESAEVTASAECIACGACV
                     YTCPKEKALFFGSRSKVLSVTAAGLLGIALFLGGYGLSRAAGIWSTYAPPPADQIAQD
                     PTRAVFGWMDLRKVSETVGLPAEKILEITGLSADTPLDKPIKQITDDEAFRKALADWF
                     AARKESQTETLSPRPSLNPEEIKGTMTLRQVAQTYGLEGRKILEKAGWPADLPPDKPL
                     KEIAAEIGKEVSEIRSAVRELLGTP"
     gene            1138..>2628
                     /locus_tag="PLY83_05055"
     CDS             1138..>2628
                     /locus_tag="PLY83_05055"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOV29435.1"
                     /translation="MNETCLLIRGGTVWTGDRRNPRAEAVLLEGERVLAVGSAADLEN
                     LEGARGARILELAGETVIPGMTDAHLHLLYYSKQRAGVSLFETRSLEEAIGVLREAAA
                     KTQPGDWIHGVRYNDAYWIENRAPTRHDLDAAGIDRPILLTRVCTHVQVANSAALAAS
                     GIPEERWGDGILLETEVAPVLKAMETWRAETGKTLGMIEKACRDFASYGVTEIQTCSA
                     SGYGLADDLAPFQRLRLEGRLPVRVVLYEDRLPQWNFMSGLGDDWIRYGGFKLFLDGS
                     LGGRTAALTSPYADDPSTCGLLNHEEEALLATVREAHKRGLQMLIHAIGDRALDQAIR
                     AVKAAYAEGPCPCGLPHRINHVMICRPDQLEALKTLGAVLDVQPAFVPSEIAMATARL
                     GEERIPWSYPWKSFVDSGLVVTGSSDCPVEPPSPWRGIWAAVCRVGDDGHPEGGWQPH
                     QKLNLEEALELWTVNGAVAVGEGGRRGRIAPGYLADLAVLDRDIFRI"
BASE COUNT          514 a          839 c          816 g          459 t
ORIGIN      
        1 ctgggaaccc tcggagaatg gtcggccaaa ctgtcacgga aactgggaat ccggcagaag
       61 gaactcccgg ggcaggtcga ccgcccgctg cgcttcctga aatacgcgat ccttgccgcg
      121 atcatcggct tcacctggaa actcggcacc ctcgcctgga gggcttacga tccctgggtc
      181 gcctggatgc acctttcagg aggcctcgat gcgatggccg aggctccctg ggccttcgcg
      241 gtccttttcg tcacggtttt cggcgccagc gttttcatcg agcggttctg gtgccgctac
      301 ctttgccctc ttggagcgct tttggccccc ctgcagaaac tttccttttt caaggtgcgc
      361 cgttccgagg agcactgcat tcactgccat ctttgcgcac aggcatgccc cgtgcgactc
      421 gacccggaat ccgccgaggt cacggcatcg gcggagtgca ttgcctgcgg agcctgcgtt
      481 tacacctgcc cgaaggaaaa ggcccttttc tttggaagcc ggtcgaaggt tctatccgta
      541 accgccgcgg gccttttggg aatcgcgctg tttctcggcg gttatggcct ctcccgcgct
      601 gccgggatct ggtcgaccta cgctccgcct ccggccgacc agattgcaca ggacccgacc
      661 cgtgccgttt tcggatggat ggaccttcgc aaggtttctg aaaccgtagg cctcccggca
      721 gagaagattc tcgaaatcac cggactttca gccgatacgc cgctggacaa gccgatcaag
      781 cagatcacgg acgacgaagc cttcagaaag gccctggcgg actggtttgc cgcccggaag
      841 gaaagccaaa ccgaaaccct ttcgccccgc ccgtccctga acccggagga aatcaagggc
      901 accatgaccc tgcggcaggt cgcccaaacc tacggcctgg agggcaggaa aatcctcgaa
      961 aaggctggct ggcccgcaga cctgccccct gacaaaccgc tgaaggaaat cgccgccgaa
     1021 atcgggaagg aagtctccga gatccgctcg gcggtaaggg aacttcttgg aaccccctga
     1081 aacatctgct ttggtataat gaaacgaaat ttcgcacatc aaggaggagg gaaccaagtg
     1141 aatgaaacgt gcctgctgat ccgcggagga acggtatgga ccggggatcg ccggaatccg
     1201 cgagcggagg ctgtccttct cgaaggagaa agggtccttg ccgtcggttc agccgcagat
     1261 ctggaaaacc tcgaaggcgc aaggggcgcg aggatcctcg aattggcagg ggaaacggtg
     1321 atcccgggaa tgaccgacgc ccacctccat ctcctttatt actcaaaaca aagggccggt
     1381 gtctccctct tcgagacaag atcgctggag gaagccatcg gggtccttcg ggaggctgcg
     1441 gccaaaacgc agccgggaga ctggatccac ggcgtccgct acaacgatgc ctactggatt
     1501 gagaaccggg ccccaacccg ccatgacctg gatgccgcgg gaatcgacag gcccattctc
     1561 ctaacgcggg tttgcaccca cgtgcaggtt gcaaacagtg ccgccctggc ggcttccggc
     1621 atccctgaag aacggtgggg agacggaatc ctcctggaaa ccgaggtggc accggtcctc
     1681 aaggcaatgg aaacctggag ggccgaaacc ggcaaaaccc tggggatgat cgaaaaggcc
     1741 tgccgagatt ttgcgagcta cggcgtgacc gagatccaga cctgcagcgc gtcgggctat
     1801 ggactggccg acgaccttgc acctttccag cgcctccgcc tggagggaag gcttcctgtt
     1861 cgagtggtcc tttacgaaga ccggctgccc cagtggaatt ttatgagcgg ccttggagac
     1921 gactggatcc gttacggcgg cttcaagctc tttctcgacg gctctctggg agggcgaacc
     1981 gccgccttga cctcccctta cgccgacgac ccctcaacct gcggactcct caaccacgaa
     2041 gaagaggcgc tcctggccac cgtgagggaa gcccacaaac gagggctgca gatgctcatc
     2101 catgccatcg gcgaccgcgc cctcgatcaa gccatccgtg cggtgaaggc cgcctacgcg
     2161 gagggaccgt gcccgtgcgg ccttccgcac cggatcaacc acgtcatgat ctgccggccg
     2221 gaccagctgg aggccctgaa gacgctgggg gccgttctgg acgtgcagcc ggccttcgtc
     2281 ccgagcgaga tcgccatggc cacggcccgc ctgggcgagg agaggatccc ctggagctat
     2341 ccctggaagt ccttcgtgga ttccgggctc gttgtcacgg ggtccagcga ctgccccgtc
     2401 gaaccgccct ccccctggcg ggggatctgg gcagccgtct gccgggtggg ggacgacggc
     2461 catccggaag gcggctggca accgcaccag aaactgaacc tggaggaagc cctggaactg
     2521 tggaccgtca acggcgccgt cgcggtggga gaaggcgggc ggcgcgggcg gatcgccccc
     2581 ggttacctgg cggacctggc ggtcctcgac cgggacatct tccggatc
//
LOCUS       DAOOVN010000058         2558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_22012_length_2558_cov_11.836596, whole genome shotgun
            sequence.
ACCESSION   DAOOVN010000058 DAOOVN010000000
VERSION     DAOOVN010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22012_length_2558_cov_11.836596"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PLY83_05060"
     CDS             complement(<1..397)
                     /locus_tag="PLY83_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOV29436.1"
                     /translation="MRLRRTMLYLPGNNPNMLLRGHLFAPDGLILDLEDAVAVREKDA
                     ARILVRDVLKRGEFGSCEVTVRINGLDTEYWRKDLEMLVPLGRLDGVRAPKVDDAATV
                     RRLDEALSEIEEKAGIEVGRTKIFCLLETA"
     gene            complement(394..669)
                     /gene="citD"
                     /locus_tag="PLY83_05065"
     CDS             complement(394..669)
                     /gene="citD"
                     /locus_tag="PLY83_05065"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HOV29437.1"
                     /translation="MKTAQAGTVESMDCLVTVSEAEPGTGLSLTLAGSSVARFSKAME
                     KAVRETLESLGIRDLAVSVQDNGALDLVLKARVEAAVARLKEGGELA"
     gene            complement(759..2009)
                     /locus_tag="PLY83_05070"
     CDS             complement(759..2009)
                     /locus_tag="PLY83_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HOV29438.1"
                     /translation="MDIRNVLRVKETKPRYKVAYIPGDDAGFDVMEGAMIVLQAMDKA
                     FGLPIDWVRADMGWCMWAKSCQEYPEGDPRRNTVPEETKQILRESDAALMAAITSKAG
                     VKGFKSAILQMRQLFDLYINLRPAKSMPGITCPLKGDPKIDLVMFRENTEDLYAAVEF
                     RPTPKEHFDMHPGMARFKDKEEVAVSWKVFSKEGCERIIRAAFEYAKATGRTKVVCAT
                     KANVIRATDGMFKEIFLNMAKEYPDIQAIDENADATAMWLIKNPQDYQVIVTPNVFGD
                     ILSDEASQLVGGMGFAPSGNIGKDFALFEPSHGSVPKYAHQYKINPCGLLNAATMMLE
                     YLGLEEAARKLEAAISAVLVEGKDLTYDVFRDNGDPDWEKKAVSTLDMARAIARKILP
                     DYDKYDAEISKKAKEMCAWEHLPQ"
     gene            complement(2077..>2558)
                     /locus_tag="PLY83_05075"
     CDS             complement(2077..>2558)
                     /locus_tag="PLY83_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HOV29439.1"
                     /translation="WKYGDDVNTDVIFAGKYTYTIKDRAEMAKVALEDLDPEFTKNAL
                     PGDIIVAGKNWGCGSSREQAVTCLKERGIGAIIAKGFARIYYRNCLNEGLPIIVSPEA
                     VDAINAGETVEIDFDASEIRTAGGKVFKFPPYPEFVRGLIEDGGLIPHVKKSLGISK"
BASE COUNT          392 a          789 c          811 g          566 t
ORIGIN      
        1 tcgccgtttc gagcaggcag aagatcttgg tcctgccgac ctcgatcccg gctttttcct
       61 cgatctcgct gagcgcttcg tccaggcgcc ggaccgtggc ggcgtcgtcg accttcgggg
      121 cgcggactcc gtccagcctg cccagcggaa ccagcatctc caggtctttt ctccagtatt
      181 cagtgtccag cccgttgatt cggacggtga cctcgcaaga gccgaactcc ccgcgtttca
      241 ggacgtcccg gaccagaatc cgggccgcgt ccttttcccg gactgcaacg gcatcttcca
      301 ggtccaggat cagcccatcg ggagcaaaaa ggtggccgcg aagcagcatg ttgggattgt
      361 tcccgggaag gtagagcatc gttctgcgga gcctcatgcc aattcgcccc cttccttcag
      421 ccgggcgacc gcggcttcga cccgggcctt caggacgaga tccagggccc cgttgtcctg
      481 cacgctgacg gccaggtccc ggatcccgag gctttcaagc gtttctcgga cggccttctc
      541 catggccttc gaaaatcggg ccacgctcga tccagccagg gtcagggaga gccccgtgcc
      601 cggctcggct tcggaaaccg tgactaggca gtccatggat tccacggttc cggcctgtgc
      661 ggttttcatg gttgcaacct ccttcgcgtt ttcgttctgt gatgcgaaag gggcccaccg
      721 cggagcagtg ggcccctcgg ggtccgcgta tgccgccctt actggggcag gtgctcccag
      781 gcgcacatct ccttcgcttt cttggagatt tcggcatcgt acttgtcgta gtccgggagg
      841 atcttccggg cgatggcccg ggccatgtcg agcgtcgaga cggccttctt ctcccagtcc
      901 ggatcgccgt tgtcgcggaa cacgtcatag gtcaggtcct tgccctcgac gaggaccgcg
      961 ctgatggcgg cctcaagctt cctggcggcc tcttcgaggc cgaggtactc gagcatcatg
     1021 gtggcggcgt tcaggaggcc gcagggattg atcttgtact ggtgggcata cttcggcacg
     1081 gaaccgtggc tgggttcgaa gagggcgaag tccttcccga tgttgccgct cggggcgaat
     1141 cccataccgc cgacgagctg gctggcctcg tcggagagga tgtcgccgaa gacgttcggc
     1201 gtgacgatga cctggtagtc ctgcgggttc ttgatgagcc acatggcggt ggcgtcggcg
     1261 ttctcgtcga tggcctggat atcggggtat tccttcgcca tgttcaggaa gatctccttg
     1321 aacatgccgt ccgtggcgcg gatgacgttg gccttggtgg cgcagacgac cttggtccgg
     1381 ccggtggcct tggcgtactc gaaggcggcg cgaatgatgc gctcgcagcc ttccttggag
     1441 aaaaccttcc aggaaacggc gacttcttcc ttgtccttga agcgggccat gccggggtgc
     1501 atgtcgaagt gttccttcgg ggtcgggcgg aactcgacgg ccgcgtaaag gtcttcggtg
     1561 ttctcacgga acatgacgag gtcgatcttc ggatcgcctt tcagggggca ggtgatgccg
     1621 ggcatcgact ttgcggggcg caggttgatg tagaggtcga acagctggcg catctgcagg
     1681 atggcggact tgaatccctt cacgccggcc ttggaagtga tggcggccat gagagcggcg
     1741 tcgctctccc ggaggatctg cttcgtttcc tcggggaccg tgttgcgcct cgggtcgccc
     1801 tcggggtatt cctggcagga ttttgcccac atgcaccagc ccatgtcggc ccgcacccag
     1861 tcgatcggca ggccgaaggc cttgtccatg gcctggagca cgatcatggc gccttccatg
     1921 acgtcgaaac cggcgtcgtc tccggggatg tacgcgacct tgtagcgggg cttggtctct
     1981 ttcactctca gaacgttgcg gatatccata cggctttacc ctcccttgaa ttggtgtttt
     2041 caacagggcg cgggagatcc tcccgcgccc ttgagcttac tttgaaatcc ccagggattt
     2101 tttcacgtgg gggatcagcc cgccgtcctc gatgaggccc cggacgaact cggggtaggg
     2161 cgggaacttg aagaccttcc cgcccgcggt cctgatctcg ctcgcgtcga aatcgatctc
     2221 caccgtttcg cccgcgttga tcgcgtcgac ggcttcggga gacacgatga tcggcaagcc
     2281 ctcgttcagg cagttgcggt agtagatgcg ggcgaagccc ttggcgatga tggccccgat
     2341 gccccgctcc ttcaggcagg tgaccgcctg ctctcggctg gacccgcatc cccagttctt
     2401 tccggcgacg atgatgtcgc ccggcagtgc gtttttcgtg aattccggat ccaggtcttc
     2461 cagggcgacc ttggccatct cggcgcgatc cttgatcgtg taggtgtact tcccggcgaa
     2521 gatgacgtcg gtgtttacgt catccccgta tttccaga
//
LOCUS       DAOOVN010000059         2448 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_23398_length_2448_cov_12.330130, whole genome shotgun
            sequence.
ACCESSION   DAOOVN010000059 DAOOVN010000000
VERSION     DAOOVN010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23398_length_2448_cov_12.330130"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..1331)
                     /locus_tag="PLY83_05080"
     CDS             complement(3..1331)
                     /locus_tag="PLY83_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOV29440.1"
                     /translation="MMSKDERIEKAARPAQEAMELHPFYRGKMQTAPKCCVRDLGDFA
                     IWYTPGVAAPCKAISEDVSLSYEYTNRANLVAVVSDGTRVLGLGDIGPEAGMPVMEGK
                     ALLFKYLGGVDAVAICLDTKDPDKIIETVRILAPSFGGINLEDIEQPKCFYILDELRK
                     DCKIPVWHDDQQGTAAVNLAGLYSGLKIVGKKIDEVKVRFFGAGAASVATARILIKAG
                     VKPGNIIMADSRGTLHKGRTDLEGDYGVKRHFAQTTNPEGVTGGPEVLFRDADVVYCY
                     TKPGPDVVKPEWVRSMARDAILFACANPVPEIWPWEAKEAGARIVATGRSDFPNQVNN
                     SLGFPGIFRGTLDVQASTITDEMCIEAARTLSACAEKVGLSEDYLIPTMDQAWVYPEV
                     AAAVGMKAIEQGIARKTYTREELLKIATEKIQYAQRMTGCLTENGFIRLP"
     gene            complement(1328..>2448)
                     /locus_tag="PLY83_05085"
     CDS             complement(1328..>2448)
                     /locus_tag="PLY83_05085"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HOV29441.1"
                     /translation="GSGTQSNMNVNEVLANRANQILNDPERAVHPNNHVNKSQSSNDT
                     FPAAMHIAAVTELEDRLLPALEKLRGTLAEKADRYMDLVKTGRTHLQDATPLTLGQEI
                     SGWVRMLERDGQMIRSSLEYLRDLALGGTAVGTGLNAPEGFDVEVAAQISLLTGKSFR
                     TAPNKFHSLTSKDELVFAHGALKALAADLLKIVNDVRWLASGPRCGIGEIRIPENEPG
                     SSIMPGKVNPTQCEAVSMVAAQVMGNDVTVAFAASQGNFELNVYMPVLIYNFLQSVRL
                     LSDAMVSFNDHCAVGIEADEKRIASFVTNSLMNVTALNPIIGYDKAAEIAKKAHREGI
                     TLKEAALALGALTEEEFDRAMDLKAMCRPRKAEGGKER"
BASE COUNT          350 a          824 c          769 g          505 t
ORIGIN      
        1 tcctacggga gccggatgaa cccgttctcg gtgaggcagc ccgtcattcg ctgggcgtac
       61 tggatcttct ccgtggcgat cttcagcagt tcttcccggg tgtaggtctt ccgggcgatc
      121 ccctgctcga tggccttcat gcctaccgcc gccgccactt ccgggtaaac ccaggcctgg
      181 tccatggtcg ggatcaggta gtcttcggag aggcccactt tctccgcgca ggcggaaagg
      241 gtccgggccg cctcgatgca catctcgtcc gtgatcgtgg aagcctggac gtccagggtc
      301 ccccggaaga tgcccgggaa gcccagggag ttgttgacct ggttcgggaa gtcggagcgg
      361 cccgtcgcca cgatgcgggc cccggcttcc ttcgcttccc agggccagat ctcggggacc
      421 gggttggcgc aggcgaagag gatcgcgtcg cgggccatgc tgcggaccca ctcgggcttc
      481 accacgtcgg gccccggctt ggtgtagcag tagaccacgt ccgcgtccct gaagagcacc
      541 tccggccccc cggtgacccc ctccgggttg gtggtctggg cgaagtggcg cttgacgccg
      601 taatctccct ccaggtccgt ccttcccttg tggagcgttc cccggctgtc ggccatgatg
      661 atgttcccgg gcttcacccc ggccttgatc aggatcctcg ccgtggcgac gctggccgcg
      721 ccggcgccga agaagcgcac cttcacctcg tcgatcttct tgccgacgat cttcagaccg
      781 ctgtacagcc ccgccaggtt cacggccgcg gttccctgct ggtcgtcgtg ccagacggga
      841 atcttgcagt ccttgcgaag ctcgtcgagg atgtagaaac acttgggctg ctcgatgtcc
      901 tcgaggttga tcccaccgaa gctgggcgcc aggatccgca cggtttcgat gatcttgtcg
      961 gggtccttcg tgtccaggca gatcgcgacg gcgtccaccc ctcccaggta cttgaacagc
     1021 agcgcctttc cctccataac gggcatgccc gcttcaggtc cgatgtcccc cagccccagg
     1081 accctcgtcc cgtcgctcac caccgccacc aggtttgccc ggttggtgta ctcgtacgag
     1141 agagaaacgt cctcgctgat ggccttgcag ggggcggcaa cccccggggt gtaccagatg
     1201 gcgaagtccc ccaggtccct cacgcagcat ttcggggcgg tctgcatctt cccccggtag
     1261 aaggggtgca gctccatggc ctcctgggcc ggcctggccg ccttttcgat ccgttcgtcc
     1321 tttgacatca tcgctctttt cctccttcgg ctttccgtgg acggcacatg gccttcaggt
     1381 ccatcgccct gtcgaactct tcttccgtga gggcccccag cgcgagcgca gcctccttca
     1441 gggtgattcc ctccctgtgc gccttcttcg cgatctccgc cgccttgtcg tagccgatga
     1501 tcgggttcag ggccgtcacg ttcatcaggg agttcgtcac gaacgaagcg atgcgcttct
     1561 cgtccgcctc gatcccgacg gcgcaatggt cgttgaagga gaccatggcg tcggacagca
     1621 gccggaccga ctgcaggaag ttatagatca gcacgggcat gtagacgttc agctcgaaat
     1681 tcccctggct ggctgcaaag gcgaccgtca cgtcgttgcc catgacttgc gcggcgacca
     1741 tggacacggc ctcgcactgg gtagggttga ccttccccgg catgatggag ctgcccggtt
     1801 cgttctccgg gatccggatc tcgccgatcc cgcagcgggg ccccgaagcc agccagcgca
     1861 cgtcgttgac gatcttcagg aggtcggccg ccagggcctt cagcgcgccg tgggcgaaca
     1921 cgagctcgtc cttgctggtc agggaatgaa acttgttcgg ggccgtccgg aagcttttgc
     1981 cggtcaaaag cgagatctgg gccgccacct ccacgtcgaa gccctccggg gcgttcagcc
     2041 ccgttccgac ggcggtaccc ccgagggcca ggtcccgcag gtactccagg ctcgaacgga
     2101 tcatctgccc gtcgcgctcc agcatcctca cccagccgct gatctcctgg ccgagggtga
     2161 ggggcgtggc gtcctgaagg tgggtgcgcc ccgtcttcac caggtccatg taccggtcgg
     2221 ccttttcggc gagggttcct ctcagcttct ccagcgcggg gagaaggcgg tcttccagct
     2281 cggtcacggc cgcgatgtgc atggcggccg ggaaggtgtc gttggaactc tgggacttgt
     2341 tcacgtggtt gttggggtga acggcccttt cggggtcgtt caggatctgg ttcgcccggt
     2401 tggccaggac ctcgttcacg ttcatgttcg actgggtccc gcttccgg
//
LOCUS       DAOOVN010000060         2436 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_23556_length_2436_cov_15.578328, whole genome shotgun
            sequence.
ACCESSION   DAOOVN010000060 DAOOVN010000000
VERSION     DAOOVN010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23556_length_2436_cov_15.578328"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..942
                     /locus_tag="PLY83_05090"
     CDS             31..942
                     /locus_tag="PLY83_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOV29442.1"
                     /translation="MKEMVVAGCQFSIVPMDVEGNIAKALTFLEKAVHEHKADLVVFP
                     ETITTGFTPLPKANQMETIASLWETVSEVPGKVTAPIQEAAARLGVHVVWATYRRGPA
                     KDVVYNSAMVIDDKGEICGQIYDKTHPFPTERKEGGGWTTAGKDPVVVETKLGRIGLT
                     ICYDGDFPELARVETLNGAEIIVRPSALLRTYDIWQFVNQARAYDNHVYYVAVNSVGP
                     DGSGNSFFGSSMIIDPTGWKLAQARGCDEIISARLNPDPIKFVSMGTRSPMIFDHVED
                     RNVGAYRDILKEGRCAFEPSKRIPYAR"
     gene            1186..2373
                     /locus_tag="PLY83_05095"
     CDS             1186..2373
                     /locus_tag="PLY83_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HOV29443.1"
                     /translation="MKKALENIRVLDFTRVLAGPFCTMMLADLGAEVIKVERPQEGDD
                     ARSFGPFVGKESAYFMSINRNKKSICLNLKNRDAIAVVKRMIPKIDVVVENFRPGVMD
                     KLGLGYEELRKLNPGLIYATSSGFGSTGPYSQLPAYDLILQGMGGIMSITGEDADHPI
                     KVGSSIADIFAGVFCAIGILAALNHREKTGEGQMVDVSMLDCQVAILENAISRYFVSG
                     ENPVPIGNRHPSIAPFATLHTASGFLNIAVGNDSLWEKFCQLIGKPELVKDPRFVDND
                     SRNRNWDSLKPILEDVLRKETKESWIERLQAAGVPCGPINTIADVVKDPQVLHREMIV
                     EIEHPVAGKLKMPGCPIKLSETPASVEKPSPTLGQHQEEILCGFLGLDREEFERLQNS
                     GAF"
BASE COUNT          570 a          646 c          744 g          476 t
ORIGIN      
        1 ccgaaccaat caagtgggag ggaagaatcg atgaaggaaa tggttgtcgc cggatgccag
       61 ttttcgatcg taccgatgga cgtggaaggg aatatcgcca aggccctgac tttccttgaa
      121 aaagcagtcc atgagcacaa agccgacctg gtcgtttttc ccgaaacgat cacgacggga
      181 ttcacgcctc ttcccaaggc caaccagatg gaaacgatcg cctcgctctg ggagaccgtc
      241 agcgaagttc ccggcaaggt gacggctccg atccaggaag ccgcggcgcg cctgggtgtc
      301 catgtcgtgt gggcgactta ccgcagggga ccggcaaaag acgttgtgta taactcggcg
      361 atggtgatcg atgacaaagg ggaaatttgc ggtcaaatct acgacaagac ccatcccttc
      421 ccgaccgagc gcaaggaagg cggcgggtgg accaccgccg ggaaagaccc ggtcgtcgtt
      481 gagacgaaac tcggccgcat cgggctgacg atctgctacg acggcgactt cccggagctg
      541 gcccgagtgg aaaccctgaa cggtgccgaa atcatcgttc gtccctcggc tcttctgcga
      601 acctacgata tctggcagtt tgtgaaccag gcgagggcct atgacaacca tgtctactac
      661 gtggccgtca actccgtggg gccggacgga tcggggaaca gtttcttcgg gagctcgatg
      721 atcattgacc cgaccgggtg gaaactggcc caggcgaggg ggtgcgacga gatcatctca
      781 gcgcggctca acccggaccc gatcaagttc gtcagcatgg ggacgagatc gccgatgatt
      841 tttgaccatg tcgaagatcg aaatgtcggt gcctatcggg acatcctgaa ggaagggcga
      901 tgcgccttcg agccctcgaa gaggattccg tacgcacgct gagacgggaa aaccgcaacg
      961 aagaatctga aacgttagcg gatgcaaggg gaggaagtgc ccgagatttt tccgcttcct
     1021 cccttttttc tttttttcgc ttctttcgaa aagttacccc ttaccagcga tcggggatga
     1081 gccttgtcaa ccctgcacag agaattcttc gggctcattc gttatgatca gtctcgcgga
     1141 cctcttggct ttgaatcggg aaatgacgca caaggaggag gaatcatgaa aaaagctctg
     1201 gaaaacattc gggttcttga ttttacaagg gtccttgcgg ggccgttctg caccatgatg
     1261 cttgccgacc tgggggcgga agtgatcaag gtcgagcgtc cgcaggaagg cgacgacgcc
     1321 cgatccttcg gcccctttgt cggcaaggaa agcgcgtatt tcatgagcat caaccggaac
     1381 aagaaaagca tttgcctgaa cctgaaaaac agggatgcga tcgcggtcgt caagcggatg
     1441 atccccaaaa ttgacgtggt ggtcgaaaat ttccgcccgg gggtgatgga caagcttggg
     1501 ttggggtacg aggaactccg aaagctgaac ccgggcttga tttacgccac ctcaagcgga
     1561 tttggatcga cgggaccgta ctcccagctt cccgcgtatg acctgatcct gcagggaatg
     1621 gggggaatca tgagcatcac gggggaggat gccgaccatc cgatcaaggt cggaagctcg
     1681 atcgccgata tttttgccgg cgtcttctgc gccatcggga ttctcgcagc cctgaaccac
     1741 cgggaaaaga ccggcgaagg gcaaatggtc gatgtctcga tgctggactg ccaggtggca
     1801 attctcgaaa acgcgatttc ccggtacttt gtgagcgggg agaacccggt ccccatcggg
     1861 aaccggcacc cgagcatcgc tcccttcgcc accctgcaca cggcttcggg gttcctgaat
     1921 atcgccgtgg ggaacgactc cctttgggag aagttctgcc agctgatcgg gaaacccgag
     1981 ctggtgaagg atccccgttt tgtggacaat gacagccgga acaggaactg ggacagcctg
     2041 aagccgatcc tcgaagacgt tctgaggaag gaaacgaaag aaagctggat cgaaaggctc
     2101 caggcggcag gggtgccctg cggtcccatc aacacgattg ccgacgtggt gaaggatccc
     2161 caggtcctcc acagggaaat gatcgtggag atcgaacacc ctgtcgcggg gaaattaaag
     2221 atgccgggat gcccgatcaa gctttcggaa accccggctt ccgtcgaaaa gccttccccg
     2281 acccttggcc agcaccagga agaaatcctt tgtggtttcc ttggattgga tcgggaggag
     2341 tttgaacggc tgcaaaattc cggggccttt taaccggtat cgcgagaagc gcgggaggaa
     2401 tggtgcaatg tcggagaaga gcatcgggga atactg
//
LOCUS       DAOOVN010000061         2336 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_25008_length_2336_cov_16.489698, whole genome shotgun
            sequence.
ACCESSION   DAOOVN010000061 DAOOVN010000000
VERSION     DAOOVN010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25008_length_2336_cov_16.489698"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="PLY83_05100"
     CDS             complement(<1..1004)
                     /locus_tag="PLY83_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOV29444.1"
                     /translation="MRDYRTIPLWRDVTEQQWRDWHWQVSNRITSVEQLKQVLPLSDE
                     EEQVITHSLNKLRMAITPYYASLINPEDPEDPIRKQAVPTIHETHIASSDLEDPCHED
                     TDSPVPGLTHRYPDRGILMVTDQCSMYCRHCTRRRKAGETDKAYSKEQISACLDYLRK
                     TPTFRDILFTGGDPFLMSDEMIDWMLGEVRKIPHIEIARFGTRTPVVMPQRITDDLCA
                     ILKKYHPVWVNTHFNHPNEITPASKEACEKLADAGVPLGNQSVLLKGVNDCPYTFREL
                     NQKLLTIRVRPYYIYQCDLSQGIEHFRTSVAKGIEIMEYLRGHTSGLAVPYFIVDGPN
                     G"
     gene            complement(1095..2240)
                     /locus_tag="PLY83_05105"
     CDS             complement(1095..2240)
                     /locus_tag="PLY83_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOV29445.1"
                     /translation="MKAVQLGCGLVGSVIARDLAMDYDVTVVDLNEKALEALKSKYPA
                     IKTVVGSCTDRELLEPLLRDADIVTAGVPGRFGYKMMETVIELGRNLSDISFMAEDFE
                     LLDGLAKEKGVVVIPDIGVAPGMSNYLMGRGCALLDEVEDAVIYVGGIPEKKVPPFNY
                     QVTWSPADVIEEYTRPARFVEDGKPVTVEAMDRLTLREFPKVGVLETFITDGLRSLIK
                     NLKAKRMEERTLRWPGHVEQMRLLRSMGLFDETPRKLGGVEVVPRDVACDLLFPMWKM
                     EPEKGDRDLTIMQVEVHGFKGKDEITYTWDLWDRFDEQSWTNSMGRCTGCTCAIFARA
                     IQKGLIKEKGVLAPEKLAADDALYEFVMKEQAARGLVYKETVTIKKG"
BASE COUNT          470 a          683 c          628 g          555 t
ORIGIN      
        1 ccattgggcc cgtcgacgat gaaataggga acggcgagcc cggaagtgtg ccccctgagg
       61 tactccatga tctcgattcc cttggccaca gaggtcctga aatgttcgat cccctgagaa
      121 aggtcgcatt ggtagatgta atagggacgg accctgatcg tcagcagttt ctggttaagt
      181 tccctgaacg tgtacgggca gtcgttgacc cctttcagga gaacggactg attcccaagg
      241 ggaacccccg catcggccag cttctcgcag gcctccttgg aagcgggcgt aatctcgttg
      301 ggatgattga aatgcgtgtt cacccagacg ggatgatatt tcttcaggat tgcgcaaagg
      361 tcatccgtga tgcgctgggg catgacgacc ggggtgcggg ttccaaagcg ggcgatttcg
      421 atatggggga tcttcctcac ttcgccaagc atccagtcga tcatctcgtc gctcatgagg
      481 aagggatctc cgcccgtaaa gaggatatcc ctgaacgtcg gcgtcttcct gaggtagtcg
      541 aggcaggccg agatctgttc cttggaatag gccttgtccg tttcccccgc ttttctcctc
      601 cgggtgcagt gacggcagta catggagcac tggtcggtga ccatcaggat tccccggtcc
      661 gggtaccggt gcgtcaatcc cggaacaggg gagtcggtat cctcgtggca ggggtcttcc
      721 aggtccgaag aggcgatgtg cgtttcatgg atcgtcggaa ccgcctgttt ccttatcggg
      781 tcctccggat cctccgggtt gatgagcgaa gcgtaatagg gagtgatggc catcctcagc
      841 ttgttcaggc tatgggtgat cacctgctcc tcttcgtcag aaaggggcag gacctgtttc
      901 aactgttcca cgctggtgat acggttgcta acctgccagt gccagtccct ccactgctgc
      961 tcggtcacat ctctccatag cggtatcgtt cgatagtccc tcacggtttt gcacctcctc
     1021 ttcgaaaaaa ccggcgggag atgtcccgcc ggcaaccccg tttttctgat tctataaaag
     1081 atacgccggt ttcgctagcc tttcttgatc gtgacggttt ccttgtaaac gagtcctctt
     1141 gcagcctgtt ccttcatgac gaactcgtac agagcgtcgt cggccgcgag cttttccggg
     1201 gcgagcacgc ctttttcctt gatcagtcct ttttggatgg ccctggcaaa aatggcgcaa
     1261 gtgcatccgg tgcagcgccc catcgagttt gtccaggatt gctcgtcaaa acggtcccag
     1321 agatcccacg tataggtaat ttcatccttc cctttaaatc cgtgcacttc cacctgcatg
     1381 atggtgaggt cccgatcgcc cttctccggt tccattttcc acatggggaa aagcaggtcg
     1441 cacgcaacat cgcgcggcac gacttcaacg cctcccagtt ttctcggcgt ttcgtcaaag
     1501 agccccatcg aacgcagaag acgcatctgc tcgacatgtc ccggccaacg gagcgtccgt
     1561 tcctccatcc tcttggcctt caagttcttg atgagggagc gcagcccgtc cgtgataaag
     1621 gtttccagca cgccgacttt tgggaattcc ctcagggtga gacggtccat cgcttctacc
     1681 gtaaccggct ttccatcttc cacgaagcgg gccggccggg tgtactcctc gatgacatcc
     1741 gcgggagacc aggtcacctg gtaattgaac ggcggaactt tcttttccgg aatccccccg
     1801 acgtagatga ccgcgtcttc cacctcgtcc agaagggcac aaccgcggcc catcaggtaa
     1861 ttcgacatcc ccggcgcgac tccgatatca gggatgacga caacgccttt ttccttggcg
     1921 agcccgtcga gaagctcgaa atcttctgcc atgaatgaaa tgtcgctcag gttccggccc
     1981 agttcgatga ccgtttccat catcttgtag ccaaacctgc ccgggacccc ggccgtcacg
     2041 atatcggcat cccgaagcag aggctccagg agttcccggt ccgtgcaaga accgacaacg
     2101 gtcttgatcg cagggtattt cgactttagg gcttccagag ccttttcgtt tagatcgacg
     2161 acagtgacgt catagtccat cgcgagatcc cttgcgatta cgctacccac aagaccgcag
     2221 ccgagctgaa ccgccttcat tcggatcctc tccttttcga gcagtatgga ttgagcggag
     2281 tcaagatagc atacaataat acaagaagtc aagggtatac agttcaggcg aaatgc
//
LOCUS       DAOOVN010000062         2280 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_25887_length_2280_cov_15.275955, whole genome shotgun
            sequence.
ACCESSION   DAOOVN010000062 DAOOVN010000000
VERSION     DAOOVN010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25887_length_2280_cov_15.275955"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..590
                     /locus_tag="PLY83_05110"
     CDS             186..590
                     /locus_tag="PLY83_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29446.1"
                     /translation="MNRKRHRGDCSILILLLLGLIVGGWFFLQRRDFERKTSPAFLEE
                     LRKVTVTLSEMKETRPDGKVQVYVSIRNGSGKTLDGKIAVFSREISGITLDRAVLTIM
                     PLAPGESTTLVAWLRKSTIRPDFQYELDVAFK"
     gene            587..1486
                     /locus_tag="PLY83_05115"
     CDS             587..1486
                     /locus_tag="PLY83_05115"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOV29447.1"
                     /translation="MTGGVLPSCLRQCGFIAAAVLAPDGRILDCNGALQEILKIEEPP
                     IGRPIGEFLFHGQPGLLEEGFLSPSGGSCRALLLEASDGTPVSLDVCFGQEGENLLLC
                     GPVGLPREEGLASRLAVLAAELAKKNRELEEANALITRLMNTDPLTGIANRRQFETVA
                     EKALSFSRRQNIPLSLIIADIDHFKQFNDRFGHALGDEVLVAFARMLFRSCRFEDTVC
                     RYGGEEFLVLLPGATLVDARAIAERLRENTEQISIPGLEERIRASFGVAEFLADETLE
                     ELISRTDKALYKAKAKGRNRVEG"
     gene            1549..>2280
                     /locus_tag="PLY83_05120"
     CDS             1549..>2280
                     /locus_tag="PLY83_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA activase"
                     /protein_id="HOV29448.1"
                     /translation="MKGLYAGFDIGSSAVHCAAVDESGEPVFLLPSRPHLGNPLRGLR
                     EQWAELLSVLSPDDLLLSTAFTGIGSRFIRRAFPDAVCEPESVALPRGAARAAPGKRI
                     LLHIGARDSFFFRLSYTRREAVVTEWATNTKCGGGSGTLLEKQVRRLYGKPDLLPAGE
                     PERLFRLFDEAAAEAALHPSAEAYHARCGIIIQSDLIHDQNEGFPRPLLVSRLVSTLL
                     RNFKNDVLGGTNLEGGPALVSGGLFS"
BASE COUNT          435 a          699 c          689 g          457 t
ORIGIN      
        1 cacgtggaaa cgctgaccct tctggagagg tccgtcgatc gttaaattta caaagaataa
       61 tatttatcgt atgttttgcc gggttcaccc gtcttctttt catttttgca tggagaattc
      121 cggctttccc cgtgatagaa taaacccatc cgggcgaaac gaagaaagga agggagggag
      181 ccccattgaa ccgaaagcgt caccgcgggg actgctcgat tttgatcctt ctgctcctgg
      241 gactgatcgt cggaggctgg tttttcctcc agcggcgcga tttcgagaga aaaacatctc
      301 ccgctttcct tgaagagctc agaaaagtga ccgtcaccct ttcggaaatg aaagaaaccc
      361 gcccggacgg gaaggtccag gtctatgttt ccatcaggaa cggttcggga aagaccctcg
      421 atgggaaaat cgccgttttt tcgcgcgaga tcagcgggat caccctggac cgggcggttc
      481 tgacgatcat gccgcttgcc ccgggcgaat ccacgacgct ggttgcctgg cttcggaaga
      541 gcaccatccg ccccgatttc cagtacgaac tggatgtggc tttcaaatga ctggcggcgt
      601 cctcccttcc tgccttcgcc agtgtggctt catcgcggct gcggttttgg ccccagacgg
      661 caggatcctg gactgcaacg gggcgctgca ggaaatcctg aagatcgagg agccgccgat
      721 cggcaggccg atcggcgagt tcctttttca cggccagccc ggacttctcg aagaaggatt
      781 cctttccccc tccgggggat cctgccgcgc cctgcttttg gaggcttcgg acggaacacc
      841 ggtctccctg gatgtctgct tcgggcagga aggcgaaaac ctgcttctgt gcgggccggt
      901 tgggcttccc cgggaggagg ggctggcttc gcggctggcc gtcctggcgg ccgaactggc
      961 gaaaaagaac cgggaactcg aggaagccaa cgccctcatc acccggctca tgaacaccga
     1021 cccgctgacc ggcatcgcga accgccgcca gttcgagacg gttgcggaaa aggccctttc
     1081 cttcagccgg cggcagaata tccccctttc cctgatcatc gcagacatcg atcatttcaa
     1141 gcagttcaac gaccgcttcg ggcatgccct tggagacgag gtccttgtcg ccttcgcccg
     1201 gatgcttttc cgatcctgcc gcttcgagga caccgtctgc cgctacggcg gagaggagtt
     1261 cctcgtgctt ttgcctggag ccaccctggt tgacgccagg gcgatcgccg agcggctgcg
     1321 ggaaaacacg gagcaaattt ccattccggg ccttgaagaa aggattcggg ccagcttcgg
     1381 ggtcgcggaa ttcctggcgg acgaaaccct ggaagagctg atctcgagaa cggacaaggc
     1441 cctctacaaa gccaaggcaa agggaagaaa ccgcgtggaa ggctgaagtc aggccgaccg
     1501 aagctccatc gcgtcagccc tcgaacggaa gagtggggga gggattcgat gaaaggcctt
     1561 tacgctggct tcgacatcgg ttcctcagcg gttcactgtg ccgcggttga tgaaagcgga
     1621 gagcccgttt tcctcctgcc gagcaggccc cacctgggca atccgcttcg cgggctccgc
     1681 gagcagtggg cggagcttct gagcgtattg tctccagacg atctgctgct ttccacggct
     1741 tttaccggca tcggctcccg cttcatccga agggcctttc ccgatgcggt ctgcgaaccg
     1801 gagagcgtcg ccctcccgag gggggcggct cgcgccgcgc ccgggaagcg gatcctcctg
     1861 cacatcgggg cgagggactc cttcttcttc cggctttctt acacccgccg ggaggccgtg
     1921 gtcaccgagt gggccacaaa cacgaaatgc gggggcggct cgggaaccct cctggaaaaa
     1981 caggtccggc ggctttacgg caagcctgac ctccttccgg ccggggaacc ggagcgcctc
     2041 tttcggcttt tcgacgaggc agccgcggaa gcggccctcc atccttcggc ggaagcctac
     2101 catgcccggt gcggcatcat catccagtcg gacctgattc acgaccagaa cgaagggttt
     2161 ccccggccgc tgcttgtttc aagactcgtc tcgaccctcc tgcgaaactt caagaacgac
     2221 gtcctgggag ggacgaacct ggagggagga cccgccctgg tctccggggg acttttttcg
//
LOCUS       DAOOVN010000063         2274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_25980_length_2274_cov_14.973411, whole genome shotgun
            sequence.
ACCESSION   DAOOVN010000063 DAOOVN010000000
VERSION     DAOOVN010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25980_length_2274_cov_14.973411"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="PLY83_05125"
     CDS             complement(<1..600)
                     /locus_tag="PLY83_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HOV29449.1"
                     /translation="MADNEMGNGQVQVEATLQNGNEPPETPAPKPKYSYGKLAALTVT
                     ELKKIAKELGVSGCSSCRRKEDLLVAVLKANAESQGYRFGGGTLETLPEGFGFLRPKG
                     LLPSDHDIYLSLSQIRRFGLRNGDVVWGLIRPPKEQEHYEALLRVEMVNFADPENARK
                     RPHFEALTPIFPNQRLRLETDPKEVSTRLVDLFAPIGKGQ"
     gene            complement(590..1246)
                     /gene="fsa"
                     /locus_tag="PLY83_05130"
     CDS             complement(590..1246)
                     /gene="fsa"
                     /locus_tag="PLY83_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HOV29450.1"
                     /translation="MRFFLDTANLDEIRQGVEWGVVAGVTTNPSLIAKEGVEFPSRIR
                     EIAALVEGPVSAEVLSTSTDAMVLEARRLFSLAPNVVVKVPMIPEGIAAVRALCQEGI
                     PTNVTLVFSPQQALLAAAAGATYVSPFVGRLDDIGEDGSGLVRDIVEIFDVQGIQTQV
                     IAASLRHPRHVFEAALYGAHVATVPFRVLEQMFHHPLTDSGLKKFLADWEVQKKSHGG
                     "
     gene            complement(1268..1711)
                     /locus_tag="PLY83_05135"
     CDS             complement(1268..1711)
                     /locus_tag="PLY83_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV29451.1"
                     /translation="MARIRKVALLRLMVLVGLVFVLAAWGLRHAFVREPFQLSQVIVC
                     EDLDEKLQPLNPGSSFPYGSRQLCLTFNYQGAHEGDRLRIEWLHENRLIYQESVSLPS
                     GSGNKVFYLLKEDASPLPAGKNKVTIDLNGRRRADVEFSIRPPGK"
     gene            1844..>2274
                     /locus_tag="PLY83_05140"
     CDS             1844..>2274
                     /locus_tag="PLY83_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOV29452.1"
                     /translation="MDESSRNRRVGARQIALGGLLAACVTAATLLSVPLPGFRLYFNM
                     GEGIIYTIALTRGPFYAAAAGGIGASLADLILGYPLWAPFTLVIKGLEGFLVGALRKN
                     RLLALAVGMSVMIAGYTTLAGILYGWKAAPVEFVTDVLQTG"
BASE COUNT          424 a          698 c          682 g          470 t
ORIGIN      
        1 ctgtcccttg ccgatcggag cgaacaggtc cacgaggcgg gttgaaactt ctttcgggtc
       61 ggtttcaagc ctcaggcgct ggttcgggaa gatcggcgtg agggcctcga aatgcggacg
      121 cttccgcgcg ttctccgggt cggcgaaatt gaccatttca acccggagca gggcttcgta
      181 atgttcctgc tccttcgggg ggcggatcag tccccagacg acatccccgt ttctcagtcc
      241 aaacctgcga atctgggaga gggaaaggta gatatcgtga tcgcttggaa gcagcccttt
      301 cggccggagg aacccgaatc cctcgggcag cgtttccagc gtgccgccac cgaaccggta
      361 gccctggctt tcggcattgg ctttcaggac agcgaccaga aggtcctctt tccgccggca
      421 ggaggagcag cctgagaccc ccagttcctt cgcgatcttc tttaattcgg tgacggtaag
      481 agctgccagc tttccgtagg agtacttcgg tttcggggcc ggcgtttcag ggggctcgtt
      541 tccgttttgc agggttgctt caacctggac ctggccgttg cccatttcgt tatccgccat
      601 gcgatttttt ctgaacctcc cagtctgcga ggaacttctt cagcccgctg tccgtcagcg
      661 ggtgatggaa catctgctcg agcacccgga acggcaccgt cgcgacatgg gcaccgtaga
      721 gggcggcctc gaacacgtgc cgcgggtgac gaaggcttgc agcgatgacc tgtgtctgga
      781 tcccctggac atcgaagatc tcgacgatgt cccgcaccag ccccgatccg tcctccccga
      841 tatcgtccag gcgtccgaca aagggactga cgtacgttgc ccccgcggca gccgccagca
      901 aggcctgctg cggggagaac acaagggtca cgttggtcgg gattccctcc tggcagaggg
      961 cacgaaccgc cgcgatccct tcggggatca tcggcacctt caccaccacg ttcggggcca
     1021 gggaaaagag tcggcgggcc tcaagcacca tcgcgtcggt cgatgtggat aggacctccg
     1081 cgctgaccgg tccttccacg agggcggcga tttcccggat tcgcgaggga aactcgaccc
     1141 cttccttcgc gatgagggaa gggttcgtcg tgacgccggc cacgacgccc cattccacgc
     1201 cctgccggat ttcgtccaga ttggctgtat cgaggaaaaa tctcatgttc gggttccttt
     1261 cacctttcta ttttccggga ggtctgatgg agaactcaac gtccgcccgc cggcgtccgt
     1321 tcaggtcgat ggtgactttg ttctttccgg ccggcagggg agaggcgtcc tccttcagaa
     1381 ggtagaaaac cttgttgccg ctgccggagg gaagggacac gctttcctgg taaatgagac
     1441 ggttctcgtg cagccattca atgcgcaggc gatccccttc atgggcaccc tggtagttga
     1501 aggtcaggca aagctggcgg cttccgtagg ggaaagacga accggggttg agtggctgaa
     1561 gtttttcgtc gaggtcctcg cagacgatga cttgagaaag ttgaaaaggt tccctgacga
     1621 acgcgtgacg taaaccccag gcagccagaa cgaacacaag accgacaaga accatcaggc
     1681 gaaggagagc cactttccgg attctggcca tagcttaaca cccatcctcc ccaaatggca
     1741 accagaaagg ccggattgct ccccctttct ggcactaccc cggacacttt accatgggta
     1801 tagtgggggg tgcaaccaaa ccgcacgatt gaggtgagcc cagatggatg aatcctcccg
     1861 aaaccgccgc gtcggagccc ggcagatcgc cctgggcggc ctgctggctg cctgcgtcac
     1921 cgcggcaacc ctgctgagcg ttcccctgcc gggttttcgg ctttatttca acatgggcga
     1981 ggggattatc tacaccatcg ccctgacaag gggccccttt tacgccgctg ccgccggcgg
     2041 aatcggggca tcgctggccg acctgattct cggatacccg ctctgggccc ctttcaccct
     2101 ggtcatcaag ggccttgaag ggttccttgt cggagctctc cggaaaaacc gccttcttgc
     2161 ccttgccgtg ggaatgagcg ttatgatcgc gggctacacg acccttgccg gcatccttta
     2221 cgggtggaag gccgcccccg tggagttcgt cacggacgtc ctgcaaaccg gcat
//
LOCUS       DAOOVN010000064         2148 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_28209_length_2148_cov_9.467750, whole genome shotgun sequence.
ACCESSION   DAOOVN010000064 DAOOVN010000000
VERSION     DAOOVN010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28209_length_2148_cov_9.467750"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1258)
                     /locus_tag="PLY83_05145"
     CDS             complement(<1..1258)
                     /locus_tag="PLY83_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HOV29453.1"
                     /translation="MENYGILSILPPLVAVVLAMKYKNVLVALFAGSFVGMLAVYHWN
                     PMTAVVAVIKEYILVQAADSYNSNLLVMMVFIGGFVGVVTYSGGAKAFAEKAVSWISS
                     RTKAQIATWIAGILIFFSDSASPMLVGSIFQPITDRMRVSREKLAWLLDTTASPVCIL
                     VPFIGWGIYIQGLIQKEYAALNIAESEWTVFLKAIPFQFYALGALFMVPLVAYLGIEF
                     SAMYKAEKRTRETGLPYWPEANISRVFTDVEEDENAPKARASMIVVPLVILFACLFGL
                     LIPFGFPIKKVPGPMLRTALCTGYFLGALSCIILMVTYKVKTLKDGYKMYMKGAGEMM
                     FILMILVLAWSLGSVCKTLGTASYIVSLAKGNIPGWSVPAILFLTGSLISFATGSSWG
                     TFAILMPLGIPMAHALGGSLLVSIGAV"
     gene            complement(1374..2054)
                     /locus_tag="PLY83_05150"
     CDS             complement(1374..2054)
                     /locus_tag="PLY83_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOV29454.1"
                     /translation="MSKLSAEERAHKEIVNLILSQKYAPGDRLVEAELAQELGLSRTP
                     VRNALRKLIAEGLLESQNNRSCTIPKITPADMEALFSVRVTLEGRAAAAAAKLATGAD
                     IDQLRGLIALERKLYAEGSKDLYTEVNEKIHLGIAKLSRNEYLERFIRQTFWRAELYI
                     FFFDRFYMRPVGSDAPLRDPDKSQSCREHARLVEAIAAQDSPLAESVMREHIQSTYQT
                     ITRRVFSY"
BASE COUNT          430 a          605 c          671 g          442 t
ORIGIN      
        1 gcacggcccc gatggaaacg aggagcgatc cgcccagcgc gtgggccatg gggatcccca
       61 ggggcatcag gatggcaaag gtcccccagg aactgcccgt ggcgaagctg atcagcgatc
      121 cggtcaaaaa caggatggcc ggaacgctcc agcccgggat gtttcccttg gcaagagaaa
      181 cgatgtaact ggcggtgccg agggtcttgc agaccgaccc caaagaccag gccagcacca
      241 ggatcatgag gatgaacatc atctccccgg cgcctttcat gtacatcttg tacccgtctt
      301 tcagggtttt caccttgtag gtgaccatga ggatgatgca cgagagggcg ccgaggaaat
      361 accccgtgca gagggccgtg cggagcatgg ggcccgggac cttcttgatc gggaacccga
      421 aggggatgag gagtccaaag agacaggcga acaggatgac cagggggacg acgatcatgg
      481 acgcacgggc tttcggggcg ttttcgtcct cctccacgtc ggtaaagacc cgggagatgt
      541 tggcttcggg ccagtaaggc agcccggttt cccgggtgcg tttctcggcc ttgtacatgg
      601 cagagaactc gattcccagg taggcgacga ggggaaccat gaagagggcc ccgagagcgt
      661 aaaactggaa gggaatggct ttcaggaaga ccgtccactc cgactctgcg atgttcaagg
      721 cggcgtattc tttctggatc aggccctgga tgtaaatgcc ccacccgatg aaggggacga
      781 ggatgcagac gggagaagcc gtggtgtcaa gcagccaggc gagcttttcc cggctgaccc
      841 gcatgcggtc cgtgatcggc tggaagatgg aaccgacgag catgggactg gccgagtcgg
      901 aaaagaaaat gaggatcccg gcgatccagg tggcgatctg ggccttcgtc ctgctgctga
      961 tccaggaaac ggccttctcc gcgaaggctt tcgccccgcc ggaataggta accacgccca
     1021 cgaaccctcc gatgaagacc atcatcacga ggagattgga gttgtagctg tcggctgcct
     1081 gcacgaggat gtattccttg atgaccgcga cgacggctgt catggggttc cagtgataaa
     1141 ccgccagcat gcccacgaaa ctgccggcaa agagggcaac gaggacgttc ttgtacttca
     1201 tggccaggac gacggccacc agcgggggaa ggatggatag gatgccgtag ttctccacag
     1261 ggttcacttc ctttctggtt tcttgacttg gatcggtcga attgttgcgc ggccccgttc
     1321 tttcttcttt ccccttgctt tcccctccct cgcggtgaat ctcctccctg gtgctagtag
     1381 gagaacaccc ttctcgtgat ggtctggtac gtcgactgaa tatgctctct catcaccgac
     1441 tctgcgagcg gcgaatcctg agcggcaatg gcttccacca ggcgggcgtg ttcccggcag
     1501 ctctgggact tgtcggggtc tctcagcggg gcatccgaac ccacgggccg catgtagaaa
     1561 cggtcgaaga aaaagatgta gagctcggcc ctccagaagg tctgccggat gaacctctcg
     1621 aggtattcat tcctggacag cttcgcgatc ccgaggtgga ttttctcgtt gacttccgtg
     1681 tagaggtcct tgcttccctc cgcgtagagc ttcctctcca gggcgatgag tcccctcagc
     1741 tgatcgatat cggcccccgt ggcaagcttg gcggctgccg ccgcggcgcg cccctcgagc
     1801 gtgacccgga cgctgaaaag cgcctccatg tcggcggggg tgattttcgg gatggtgcag
     1861 cttctattgt tctggctttc caggaggccc tcggcgatca gcttgcgaag cgcatttctg
     1921 accggggttc ggctcaggcc cagctcctgg gcgagttccg cctcgacgag gcggtctccc
     1981 ggcgcatact tctgggatag gatcaggttg acgatctcct tgtgtgcccg ttcctcagcg
     2041 gaaagcttgg acatccgatt attctccttt ttcactaagt tgtgtcccaa gaagtatggt
     2101 gcctaagata ctaaaatgat gggggagatt ctgtcaagac gaggccgg
//
LOCUS       DAOOVN010000065         1633 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_42559_length_1633_cov_15.266160, whole genome shotgun
            sequence.
ACCESSION   DAOOVN010000065 DAOOVN010000000
VERSION     DAOOVN010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42559_length_1633_cov_15.266160"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PLY83_05155"
     CDS             <1..213
                     /locus_tag="PLY83_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter, permease protein
                     PstA"
                     /protein_id="HOV29455.1"
                     /translation="AFFTPDIARSLFSQVMALPYHVLILATAGTNIDETRGIQYGTIL
                     VLLLVVLGITLTGVIWRARLRMRSSR"
     gene            252..1001
                     /gene="pstB"
                     /locus_tag="PLY83_05160"
     CDS             252..1001
                     /gene="pstB"
                     /locus_tag="PLY83_05160"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOV29456.1"
                     /translation="MKFQISNLNLYYGPTQALKDVSIDLPESSVTAFIGPSGCGKSTF
                     LRCLNRMNDFIPRCRVEGRVLLDGTDIYAPGTDVISLRRRVGMVFQKPNPFPTSIFDN
                     VAYGPRLHGITDRGTLEGIVRQSLEGAALWDEVRDKLSSPALSLSGGQQQRLCIARAI
                     AVQPEVLLMDEPTSALDPMSTARIEELVRELKTKYTVIIVTHNMQQAARISDLTAFFL
                     LGEMVEAGPTQDIFTSPADRRTEDYITGRFG"
     gene            1030..>1633
                     /gene="phoU"
                     /locus_tag="PLY83_05165"
     CDS             1030..>1633
                     /gene="phoU"
                     /locus_tag="PLY83_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOV29457.1"
                     /translation="MTELNVRKTQDEALDRIREQLLKLGNLAQDAIRKAVWALEKQDE
                     TLSREVLEGDDVLDALASEIEEGCLLFTARFQPVAQDLRTISAINRMAIDLERIGDLG
                     VSIAKITRFFFGKTLIKPLIDIPRMAAIVEEMIDGTLRAFINRDVEEAKRVCAMDDML
                     DDLDRQIFNELLLLMIENPRVIEQATQLVLVSRALERAGDH"
BASE COUNT          340 a          532 c          483 g          278 t
ORIGIN      
        1 gcctttttca cgccggacat cgcgagaagc ctcttcagcc aggtcatggc gcttccgtac
       61 cacgtgctga tcctggccac ggccgggacc aacatcgatg agacgcgcgg catccagtac
      121 ggaacgatcc tcgtcctgct gctggtcgtc ctcggaatca cgctgacggg cgtcatctgg
      181 cgcgcgcggc tgcggatgcg ttcctcccgc tagcctcgga aaaaaggaaa ggagaaccca
      241 cggtgtccag gatgaaattc cagatttcca acctgaatct gtattacggg ccaacccagg
      301 cgttgaagga cgtgtcgatc gacctgcccg aaagctccgt caccgccttc atcggcccct
      361 cgggctgcgg gaaaagcacg ttcctgcgct gcctgaaccg gatgaacgat ttcatcccta
      421 ggtgccgcgt ggagggccgg gtccttctcg acggaaccga catttacgcc ccgggcaccg
      481 acgtcatctc cctccgcagg agggtgggga tggttttcca gaagccgaac ccgttcccca
      541 cctccatctt cgacaacgtc gcctacggcc cccggcttca cgggatcacg gaccggggca
      601 ccctggaggg gattgtccgg cagagcctgg agggggcggc cctgtgggac gaagtccggg
      661 acaagctttc ttcccccgcg ctctcccttt caggagggca gcagcagcgc ctgtgcatcg
      721 cccgggcgat cgccgtccag ccggaggtcc tcctcatgga cgaacccacc agcgccctgg
      781 accccatgtc cacggcacga atcgaagaac tcgtcaggga attgaaaacg aagtacactg
      841 tcatcatcgt cacgcacaac atgcagcagg ctgcccgcat ttccgatctc accgcctttt
      901 tcctcctggg cgagatggtg gaggcgggac cgacccagga catcttcacc tctcctgccg
      961 accgaagaac ggaagactac atcaccggac ggttcggata ggcaagaacg gagaaaggga
     1021 gaccagacca tgaccgaact caacgtgaga aaaacgcagg acgaggcgct ggaccgcatc
     1081 cgggaacagc ttctgaagct cggcaacctg gcccaggacg ccatccggaa agccgtgtgg
     1141 gcgctcgaga agcaggacga gaccctttcg agggaagtcc tggaagggga cgacgtcctc
     1201 gatgccctgg cttccgaaat cgaagaggga tgcctgcttt tcactgcacg tttccagccg
     1261 gttgcccagg acctgcggac catttcggcg atcaaccgga tggccatcga cctggagcgg
     1321 atcggggacc tcggggtcag catcgccaag atcacccgct ttttcttcgg caagaccctc
     1381 atcaagcccc tgatcgacat cccccgaatg gccgcgatcg tcgaggaaat gatcgacggg
     1441 acgctccgcg ctttcatcaa ccgggacgtc gaggaagcga aacgggtctg cgcgatggac
     1501 gacatgctcg acgacctgga ccggcagatc ttcaacgagc tgctcctgct gatgatcgag
     1561 aatccccggg tgatcgaaca ggccacccag ctcgtcctgg tttccagggc cctggaacgg
     1621 gccggcgacc aca
//
LOCUS       DAOOVN010000066         1626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_42835_length_1626_cov_12.129217, whole genome shotgun
            sequence.
ACCESSION   DAOOVN010000066 DAOOVN010000000
VERSION     DAOOVN010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42835_length_1626_cov_12.129217"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..828)
                     /locus_tag="PLY83_05170"
     CDS             complement(4..828)
                     /locus_tag="PLY83_05170"
                     /inference="COORDINATES: protein motif:HMM:NF020476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HOV29458.1"
                     /translation="MIISASRRSDIAAFHTEWFMEQVRKGFVDVRNPYNARQVSRIPL
                     DPESVDVFVFWTRNPLPLLPYMDELEARGYRSAFLVTVMDYPRELEPATPRVPEAVEA
                     LARLFERVGKYRIAWRYDPILLSSITPASFHEEAFSRLSEQIAPYVSRCIVSVFDPYW
                     QALKRLKLLQSLGFRLFDRNEEQQALAELLPQMASVSSRLGLEIQSCCEGDRLAPWIR
                     PGACIDPLFLQEAFGRTFPSAKARGQRLGCLCAGSKDIGTYGTCRHGCLYCYAGRL"
     gene            932..>1626
                     /locus_tag="PLY83_05175"
     CDS             932..>1626
                     /locus_tag="PLY83_05175"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOV29459.1"
                     /translation="MYFKNILVAPSDCEPGKGMTLAALLNRLQDAAGEAAEELGASVP
                     RLLAEGFTWMLVKYRLRAGRMPLPGEDLRLATWHFPHGRLYSLRAFELTSGETRLVRA
                     DSCWVLVDLGRRKPVHIGKVLPSLFQAENPPIPFEFEKVESCGSGPWSASLQVEDPDI
                     DENGHVNHIRYLEWATEALPAGWRQDKAFRGFDAEFHGEGRSGERLEIFARAEPSETG
                     GHACLHGILRPSDG"
BASE COUNT          300 a          485 c          548 g          293 t
ORIGIN      
        1 ctctcacagc ctccccgcgt agcagtacag gcacccgtgc ctgcaggtgc cgtaggttcc
       61 gatgtccttg gacccggcgc agagacatcc gaggcgctga ccgcgggctt tggcggaggg
      121 gaacgttctg ccgaaggctt cctggagaaa cagggggtcg atacaggcgc ccggccggat
      181 ccagggggcc aggcggtctc cctcgcagca ggactggatc tccaggccga gccggctcga
      241 aaccgaggcc atttggggca gcagttcggc cagggcctgc tgttcctcgt ttcggtcaaa
      301 caggcgaaat cccaggcttt gaagcagctt cagccgcttc agggcctgcc agtacgggtc
      361 gaagacggaa acgatgcagc gggaaacgta cggggctatc tgttccgaaa gcctcgaaaa
      421 ggcttcctcg tgaaaggaag cgggggtgat ggaggacagc aggatcgggt catacctcca
      481 ggcaatccgg tattttccga cgcgctcaaa aagccgggcc agggcctcga cggcttccgg
      541 aacgcggggg gtggccggct cgagttcccg cgggtaatcc atgacggtca ccaggaaagc
      601 ggaccggtag ccccgagcct ccagttcatc catgtaggga agcaggggga gggggttccg
      661 cgtccagaaa acgaaaacgt cgaccgactc ggggtccagg gggatccgcg aaacctgccg
      721 ggcgttgtaa gggtttcgaa cgtccacaaa gcccttgcgg acctgttcca tgaaccattc
      781 ggtgtggaac gcggcgatgt ccgaccgtcg gctggcgctg atgatcatac cctttccccc
      841 cgttttgttg agaatggctc tccctacaat tctagcggag gcgtatgatg ggtgccgatc
      901 catttttcga ttccggggag gtttttcggg agtgtatttc aaaaacatcc ttgtcgctcc
      961 atcggattgc gagcctggga aagggatgac gctggcggcc ctgctgaacc gcttgcagga
     1021 cgccgcgggc gaggccgccg aggaattggg ggcctcggtg ccccggcttc tggccgaagg
     1081 attcacctgg atgctggtga agtaccggtt gagggctggc cggatgccgc ttccgggaga
     1141 ggacctccgg ctggccacct ggcattttcc ccacggtcgg ctgtattccc ttcgggcctt
     1201 cgaactcacc tcgggcgaaa cacgcctggt ccgggccgac agctgctggg tgctggtgga
     1261 ccttgggcgc cgcaagcccg tccacatcgg gaaagtcctg ccttcgctgt tccaggccga
     1321 gaatcctccc attccgttcg agttcgagaa agtggaatcc tgcggaagcg gcccctggtc
     1381 tgccagcctt caggtggaag acccggacat cgacgaaaac gggcacgtga accacatccg
     1441 ctacctggag tgggcaacgg aggcgctgcc tgccggctgg cggcaggaca aggccttccg
     1501 gggcttcgac gcggagttcc acggggaggg gaggtcgggc gaaaggctcg agatcttcgc
     1561 cagggccgag ccttcagaaa ccggtgggca tgcctgcctt cacggaatcc tccgcccgtc
     1621 cgacgg
//
LOCUS       DAOOVN010000067         1610 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2440
            NODE_43459_length_1610_cov_11.064952, whole genome shotgun
            sequence.
ACCESSION   DAOOVN010000067 DAOOVN010000000
VERSION     DAOOVN010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864515
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:42:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,037
            CDSs (total)                      :: 1,014
            Genes (coding)                    :: 1,013
            CDSs (with protein)               :: 1,013
            Genes (RNA)                       :: 23
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43459_length_1610_cov_11.064952"
                     /isolate="AMR_MDS_2440"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="PLY83_05180"
     CDS             complement(<1..773)
                     /locus_tag="PLY83_05180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOV29460.1"
                     /translation="MSVNKVDVAIIGGGVHGCSTAYHLARKGFKVALFERDFISSGAS
                     GRSAAGIRLQFGTEVNCLLAQYNIQVFKTLEQDLNYPLPLEYDPAGYLWIAYTEGQMA
                     QMEKNVALQNSLGSPSRMLTAEGIREVAPYLNTEGMLGGSFCQEDGHINPHTLCFAYA
                     DSAAKLGAEIYKKTSVTGLIVKNGRAAGVHTTRGDWEADAVVCCAGPWSVEVGKWAGF
                     DIPITPERHQVLITEPVERIKHPMTLCQDDGAYVKQCPNG"
     gene            complement(946..>1610)
                     /locus_tag="PLY83_05185"
     CDS             complement(946..>1610)
                     /locus_tag="PLY83_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HOV29461.1"
                     /translation="EVVLDTPAGLVRLKVEVNNGIVGDVILSNVPAFVYARDVELTVP
                     SLGRQIHLDIAYGGNFFAIVPAGQLDLSITPEECGRIVPLGLEILREVNKAVKVQHPE
                     EKAIDHVALVEFSLERPGQVTRNTVVWGEGNQDRSPCGTGTCAKMALLAAKGKLAPGE
                     KFLHQSITDSLFEGWYEPGPRVGGFETVLPYLKSRSYVTGFNFLLKQPEDKVGEGFLL
                     KR"
BASE COUNT          277 a          517 c          492 g          324 t
ORIGIN      
        1 ccgttcgggc actgcttcac gtaggcgccg tcgtcctggc agagggtcat ggggtgcttg
       61 atccgttcca cgggttccgt gatgagaacc tggtgcctct ccggcgtgat ggggatgtca
      121 aaccccgccc acttcccgac ttcaacgctc cagggcccgg cacagcagac cacggcgtcc
      181 gcctcccagt ctccacgggt ggtgtgaacc cctgccgcgc ggccgttctt gacgatgagc
      241 cccgtgacgg aagtcttctt gtagatttcc gcgccgagct tcgccgcgga gtcggcatag
      301 gcgaaacaga gggtgtgggg gttgatatgc ccatcctcct ggcagaaact gccgcccagc
      361 atcccctcgg tattgaggta gggggccact tcgcggatgc cctccgcggt gagcatccgg
      421 ctggggctgc ccaggctgtt ctgaagggcc acgttctttt ccatctgggc catctggcct
      481 tcggtgtagg cgatccagag gtaacccgcc gggtcgtact cgaggggaag cgggtagttg
      541 aggtcctgct cgagggtctt aaaaacctgg atgttgtact gggccaaaag gcagttcact
      601 tccgtgccga actgcagccg gattcccgcg gcgctgcggc cgctggcccc ggaggagata
      661 aaatcccgct cgaaaagggc gaccttgaac cccttcctgg cgagatggta ggcggtggaa
      721 cagccatgga ccccgccgcc gatgatcgca acgtcaacct tgttcacgga catgcagaaa
      781 gacctccctt ggcatcgtca ggggaaagca gactcgctct cccgggttcg ggacaagccg
      841 ctttggttca ggcaggcctt tcacacgggg gtctttttcg gcatcctctg ggcaggggca
      901 ggtgccagcc gccctggcag ttcatttcgc ttgcctcccg gccgctcagc gtttcagcag
      961 gaaaccttcc ccgaccttgt cttccggctg tttcaggagg aaattgaaac ccgtgacata
     1021 ggagcggctt ttcaggtacg gaaggaccgt ttcaaagccg cccacccggg gacccggttc
     1081 gtaccagcct tcgaaaaggc tgtccgtgat gctctggtga aggaacttct cgcccggggc
     1141 cagcttgccc ttggcggcca gaagcgccat cttggcgcag gttcccgtgc cgcaagggga
     1201 acggtcctgg ttcccctctc cccagacgac cgtgttccgc gtgacctgcc ccggtctctc
     1261 caggctgaat tccaccaggg ccacgtgatc gatggccttt tcctcggggt gctgcacctt
     1321 caccgccttg ttgacctccc tcaggatctc cagccccagg ggaacgatcc tcccgcattc
     1381 ctcgggcgtg atcgaaaggt ccagctgtcc ggccgggacg atggcaaaga aatttccccc
     1441 gtaggcgata tccaggtgga tctgccttcc cagggaagga accgtcagct cgacatccct
     1501 ggcgtagacg aaagccggga cattgctcag gatgacatcc cccacgattc cgttattcac
     1561 ctcgaccttc aggcggacca ggcccgcggg ggtgtcgagc acgacttcgg
//
