LOCUS       DAOOVW010000001        77000 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_176_length_77000_cov_7.916213, whole genome shotgun sequence.
ACCESSION   DAOOVW010000001 DAOOVW010000000
VERSION     DAOOVW010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 77000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 77000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77000
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176_length_77000_cov_7.916213"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..852
                     /locus_tag="PLW95_00005"
     CDS             169..852
                     /locus_tag="PLW95_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOV21050.1"
                     /translation="MNRRKESISVSDEYCGGGFTLIELLVVIAIIAILAAMLLPALSQ
                     AREKARQSVCMNNLKQLGLAIQMYVQDNDGWLPNMYDGTYWPTKLSMYIGGATFTFVP
                     WNPGWSAGTPKRVKDVFICPSGIKELHWGINYAYNKTIGDYTPRWGYPTVSYCDPEKL
                     DRIKNPTRVFLVIDQRTKSCSEIGNDWNAGYVDYRHNDGANVLWADSHVNWASRSEVA
                     RWRATEWVP"
     gene            982..2463
                     /locus_tag="PLW95_00010"
     CDS             982..2463
                     /locus_tag="PLW95_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21051.1"
                     /translation="MIEKNLYLSTAIPITDISSRMFFFEENQPSISFGIHKVYAPITK
                     VPIIMKGLTKNTISVSSFASTIIKLGPSHYRLYSTNYPFSFKTMYIALWESGDGIIWY
                     PLKVSRHGKFSSNSICINGIPGNQDMIGQPQVLHLADNVWYMYFWKHKEGHCRYLRAE
                     SRDGLNWAVVDFDNPVLYHPNDQGLYAWAMGISLRKIKNLQITRKEAEKHLKLISNDA
                     TYVYYNEYLKRFECYSVWLHPEVRERSRYKGEAGKPKGIIRFIHKRVSSDGINWSYPE
                     VILLPDYRDPWDLQFYYLSVQWHDKWLIGNLGYYRTEEGQDTTDMDLCFSKDGQSWHR
                     PVRGGFIPRVENKPDAMGIYAPNTWIDKGNKWLCLYTGTPDPHDEDIHRASIMGATFD
                     KNRFVGLQAGKVTGGFLSDVFILQNANIKVDACIKGWMRAEICDAFGNAIKDFHLMDS
                     YVISGDKSEHILRWKQSDTKDLKYRALRLRIEFREGVIYSFCF"
     gene            2689..3912
                     /locus_tag="PLW95_00015"
     CDS             2689..3912
                     /locus_tag="PLW95_00015"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOV21052.1"
                     /translation="MEMKVHKRILIPIKEEKHKYKEDTSFGTSIMMVNTTYTKKDGLH
                     LLQLYSEEVASDTSGKCFQRTSKDNGKTWSNPILVFEPQETEEGVIRWGESCLFLDEG
                     KECIFHFYNLHLYPKNIFSREVQEYTRIFYRISFDEGKTFSNGKQIIQKGFNETNWAE
                     DVIYGKNSIAISFCAPLKLNSGKILLPCQKVPLEHKGDNPFLISYVAGCFIGEWKGQE
                     IEWELSQMVKIDPQLSSRGLCEPTIAQLYNGELLMIMRGSNYTIPSVPGYKWFSISKD
                     SGYTWSSPTPLKYDTGENFFSPATGSRLIRSTKNKKLYWIGNIVKENPDGNRPRYPLQ
                     IAEVDEEKKAIKKHTVNIIEDKRESDSPFVQFSNFRVYEDRETGEFVLIMARIQERGE
                     KDLSSPAYEYRIEVL"
     gene            3920..4945
                     /locus_tag="PLW95_00020"
     CDS             3920..4945
                     /locus_tag="PLW95_00020"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOV21053.1"
                     /translation="MGKMKFLVIGLGSMGRRRIRCLQYLGEKDIIGFDIRKDRYEETE
                     KKYGIKTFSDFNEACGQNPNVFIISVPSNLHHFYAMEAVKRNKHFFTESNFLPEGIDD
                     LVKVEKEGKIICVPSFTMPHHPSVKIMRSGIEEGKIGKVYSFTYHLSSYLPEWHPWED
                     YRDVYYGKKETPGSKEMVAFELTWIVWLLGRVQEVCAFKSKMSSLEIEFEDTYQIILK
                     LKNGVLGHLLIDVASLPSGRNMKILGEKGTFCWNSEEEIIKWFNTEKRKWEEIKGDKG
                     IEEAGYSVHTHEEMYIEEMENFVKTVRGEKKYPYTFTQEKHIIDILVAIEQSISQKIV
                     MEVEDEV"
     gene            4935..6245
                     /locus_tag="PLW95_00025"
     CDS             4935..6245
                     /locus_tag="PLW95_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013325938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOV21054.1"
                     /translation="MRCRKGQKLWQKAKKIIPGGTQLLSKTSEQFLPEYWPSYYKKAK
                     GISVWDLDGNKFLDFSIMGVGSCILGYADPDVNKDVKKVINNGNMCTLNSPEEVELAE
                     LLLKIHPWAGKVRYARTGGEAMAIAVRIGRAFSGKDKIAFCGYHGWHDWYLSANLAGD
                     KNLDGHLLPGLKPLGVPRGLYGTAFPFTYNHIEELEVIIKNNEIGVIVMEPIRSYYPE
                     NGFLEKVRTLANKNKAVLIFDEITSGFRMKVGGAYTLFNLEPDIVVYAKAMSNGYPMA
                     AVVGRENIMDKAEESFISSTYWTEKIGPNAALATIKKMKRENVPSHLIKIGEQITNGW
                     KEIANECGLKIKISGIPPLTKFSFCYENSQELITLFTQEMLERGFLAKDSVYVSYSHK
                     ETDVEIYMSAVKEVFKKIKKGIDTNKISKMLKGPVAHTGFKRLT"
     gene            6266..7183
                     /locus_tag="PLW95_00030"
     CDS             6266..7183
                     /locus_tag="PLW95_00030"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOV21055.1"
                     /translation="MIELAFLGTNEGNGHIFSWSAIINGKYDEQLMKECGYPVIYEYL
                     SKNKKDIGIPGAKVSYVWTEDKKYSEKVAKTSFIENVIDTPEQVIGKVDGVVITTDIG
                     SNHLKLAKPFIEENIPVFIDKPLTDNEKDLKKFIEYFKEGKPILSSSSYRYSKNIDDF
                     LKENTDKIEFVRCIMNKTWERYGVHAMEGLYKIMGSGIKSVINLGDKNINVVYIEYSD
                     ERKAVIENIYNSKITDYDIITPKNTYTIDDTNTFYMFKKQMEIFIRFIDERKYPYPYE
                     ETIEITKVIIAGIISREEKRKVELKEILY"
     gene            7202..7981
                     /locus_tag="PLW95_00035"
     CDS             7202..7981
                     /locus_tag="PLW95_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOV21056.1"
                     /translation="MGILEKFSLKGKVSIVTGGAGLYGKGIVEGLAEAGGCVYVASRN
                     VENCNKLADTLRKKGYSVAGAKLDQTDTESIKQLRNRVIDEQGKIDILINNAVLRPME
                     SPDAPIETFELSMKVNATGVVNITRHIAEEMKKQRSGVIINIASIYGVLGIDPYLYEG
                     TDMGLSGPDYFFHRAGIINLTRYYASLLGPYNIRVNCISPGGLFNNQHPVFVERYNRK
                     TFLGRMANREDIKGVVVFLASDASSYITGANIMMDGGLSAK"
     gene            7978..8976
                     /locus_tag="PLW95_00040"
     CDS             7978..8976
                     /locus_tag="PLW95_00040"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOV21057.1"
                     /translation="MKKKILIIGAGGIGKKHIDGFLRTGKFEISACDVDSKKLKEVEE
                     KFKIKNLIIDFNSVNLRNFDCVLISAPANFHIPIAIKCAENNIPFLVEKPISVDLKSI
                     DKLLKIVEKNKVKCGVGFTRRSVPSFKKLKEIIENKKCGETKMAAFHVAQDYRKYRPD
                     YAEIYFAKEKMGGGCILDAVSHSIDLAQWYIGKPEYGYAIYDNLDFGEKIETEDSSAI
                     ISKFDNVIVNFYCNLFQKPYELFIEFAGTKGNVRYISENKQISKILFSDNDEGRWEEL
                     FTFENEISDYYFYQAENFLQLINGEKTSFTKLEEAVENLKFCLKLKKGGKNEIKRS"
     gene            8957..9883
                     /locus_tag="PLW95_00045"
     CDS             8957..9883
                     /locus_tag="PLW95_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21058.1"
                     /translation="MKLKEVKCIWWDGYHNAFTDIIKYKGSYFVCFRHATEHGLKGQG
                     EIYVLKSNNLENWELITKFPPLEDSRDPKFFIFEEKIGVLFFAYPDINTRQIRDGYIS
                     YSDDGENFSPVIKISKDNLCFWRIRNYKEKLYATAYKGGEKEEDWGSYLFVSDDGENF
                     NKVSTILEGEYANETDLLFEGDICYAIVRRENLQTPALCISKYPFDKWESYNLNKIIQ
                     GPCIFKFKGEIYIAGRVNTLTKTGILKLDLERKRVKDEIILPSEGDTSYCGVVVENNK
                     FYLSYYSQHEIEKRESKVGENAAGIYIAEIGE"
     gene            9886..10788
                     /locus_tag="PLW95_00050"
     CDS             9886..10788
                     /locus_tag="PLW95_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOV21059.1"
                     /translation="MEMKFFGVYPALLTPFDEKENVDEKRLRKIIKFLISKGVDGLYI
                     CGGTGEGMLMNVDERKKVAEIVKEEVGNKVKIITHIGGALNTKNTIELAKHSEQLKID
                     ALSSIPPFYYKFSFKEIYKYYKEVAENTSLPFFIYYIPVTTGVSITIDEIWELSKIKN
                     IIGIKYTNYDFFTLQNIILKTRWISFSGPDEMFLPALTMGVVGCIGSTQNVLPEIFID
                     IYKSFKEGNIKKAMEGQKRITIAVSILKKYDGITAWKATMKFREIDVGYAKKPFENFL
                     EKSKEKELKNEWKKYFPEFSEGIK"
     gene            10847..11914
                     /locus_tag="PLW95_00055"
     CDS             10847..11914
                     /locus_tag="PLW95_00055"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOV21060.1"
                     /translation="MKKIDLLPASEKNPRNSEGSMIELKNGKIMFIYSHFYGGQGDNA
                     LAFLAARYSFDKGESWTEKDEVIIENEGKENVMSVSLLRLKNGEILLGYLIKNSLNDC
                     KYYIRKSSDEGKSWSKKVLVTNKEFSNGNYFVVNNDRVIQLSNGRIIVPSSHHPCSTG
                     KWDDFGPGEVIVFYSDDNGNTWKSSRNILSSPEKNDKRGLEEPGVVELRDGKILMWIR
                     TNLGRQYYSYSEDSGQTWSEVSPSPLVSPLSPASIKRIPKTGDLLCIYNDHSGKFPYP
                     EFQSRTPLVSAISKDEGKSWQNHLLIEDDPKGRFCYTSILFIDNKIILSYSAENVEQA
                     WGLLRVTILKMEEVYNGEKNT"
     gene            11895..12332
                     /locus_tag="PLW95_00060"
     CDS             11895..12332
                     /locus_tag="PLW95_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21061.1"
                     /translation="MEKKIHKDFHGALSVGFEFLSDKYGEKVLEEYLIDAAETIYSDL
                     IKKIKKNGIVELENYWRKIFTEEEGKFFLKRDDNKKIELVVEECPAISHMKKVGYKIY
                     KNFCIQCEVINRVISQKTGFISEITYDVKNGKCKQIFKKEERT"
     gene            12329..12775
                     /locus_tag="PLW95_00065"
     CDS             12329..12775
                     /locus_tag="PLW95_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21062.1"
                     /translation="MISSGEFIFAYNELFKFIHKKYGKKDVISLWEFISDKFLTNLDE
                     LVREKGISGMAEYWTHTLTEEGAEYQMSVGENYFEIFMKKCPSVGKLNKTKVKKYPYY
                     CEHCKTLYKKVIEKYGFLYKVKIIDKEKGICKVFVKRTKKRNFPIQ"
     gene            complement(12747..13010)
                     /locus_tag="PLW95_00070"
     CDS             complement(12747..13010)
                     /locus_tag="PLW95_00070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOV21063.1"
                     /translation="MNKKKLKEKLLVELKKIKFGHTKTYSYIALKLGMKNNVRYISSI
                     LKENPYLISIPCHRVIKKNGEIGNYVLGKEFKKYLIEWENSFF"
     gene            13063..13470
                     /gene="hisI"
                     /locus_tag="PLW95_00075"
     CDS             13063..13470
                     /gene="hisI"
                     /locus_tag="PLW95_00075"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012473921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HOV21064.1"
                     /translation="MEKYIQSFIEMVKFNENGLVVVVVQDLNGIILMVAYMNTEAIEK
                     TLQTGKMHYYSRSRKKLWLKGESSGNFQFVKDIYIDCDNDALLFRVQQKCGACHSGYY
                     SCFYRKFEDGNFKIIEEKIFEPEDVYNKEIKKC"
     gene            13464..14783
                     /gene="secD"
                     /locus_tag="PLW95_00080"
     CDS             13464..14783
                     /gene="secD"
                     /locus_tag="PLW95_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017952057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOV21065.1"
                     /translation="MLKSYYYKLFLIIAIIGIFIYLIYPVDKKIKKGLDLQGGIHIVL
                     EVEKPPEETENINELTDRALEIIRNRIDALGVSEPIIQKEGANRIIVDIPGVKEPEKA
                     IDIIGKTALLEFKLVNDDPKALQSALEGNPPEGYEVLYTTEKDERGIARKGSPILVKK
                     EPELTGKYIKDAYVSYDPGGLPSVSIVFNSEGAKIFENVTTNNVGRQLAIVLDGVVKS
                     APMIRERIPEGKGQISGKFNMEEAKELAIVLRAGALPAKVNMIYQQIIGPTLGKKYIQ
                     QGFRVILYGGILVFIFMIFYYSVFGLLADFALALNILILLGIMAGIKGVLTMPGIAGI
                     ALTIGMSVDANILIFERIREEIKAGKSPKTAVDAGYLRAWSAILDSNITTLIIGAILF
                     FFGEGSIKGFGLTLSIGILANLFTAVYVTKVIVDYFLIIKKVEKIRI"
     gene            14786..15673
                     /gene="secF"
                     /locus_tag="PLW95_00085"
     CDS             14786..15673
                     /gene="secF"
                     /locus_tag="PLW95_00085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOV21066.1"
                     /translation="MELIKKTNFDFMGKRKIAYLFSIIVILWGMTTFFIRGKKNFGVD
                     FTGGDLLQIAFKNSVSVSEIRDTLSTLNIGSFTVQAVGTEEKEVIIKSNPNTSDTILK
                     AIQQKYGQDIEIKNKAIISPSMSTSLRNKALFSFLLGIIGILIYLAIRFEFRFAVGAT
                     VAIFHDLLFVISILAIAGKQIDSTIIAALLTIAGYSVNDTVVIFDRIRENVRKTRSDN
                     YIGIFNLSINETLSRTILTGLTTIVVVILFFFFGGESLHDFAFSLLGGFIIGTFSSVF
                     VASALVIDWHRIRPYKIKL"
     gene            15670..16248
                     /gene="rsmD"
                     /locus_tag="PLW95_00090"
     CDS             15670..16248
                     /gene="rsmD"
                     /locus_tag="PLW95_00090"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOV21067.1"
                     /translation="MKVIGGIHKGKKLLFIKEKDIRPTKDVVREAIFDVLRGWIVGKK
                     IIDLFAGTGALGIEAISEGGEKVIFVESKGKIVDILKKNVINVGIEKQVSILKGGVEE
                     KIKEMRNFKYDLVIADPPYDFPVNKLKEILETIISSEVINENGIIIVEHRTKYSIPES
                     AKFIVYKNKKYGKTSVSYLKRKENEEYSSLSG"
     gene            16220..16702
                     /gene="coaD"
                     /locus_tag="PLW95_00095"
     CDS             16220..16702
                     /gene="coaD"
                     /locus_tag="PLW95_00095"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOV21068.1"
                     /translation="MKNIAVYPGSFDPITNGHIDVIHRCLKIVDKIIIAISSFQRKDF
                     LLTIEERTEIIKETFKNDDRIEIDKFDGLLVNYLKEKNINIIVRGLRAISDFEYEFQM
                     ALTNRKLNKNIETIFIMPGEEYFFISSTMVKEIAKLKGQISCFVPPAVEKKLKEKFNY
                     "
     gene            16778..16972
                     /gene="rpmF"
                     /locus_tag="PLW95_00100"
     CDS             16778..16972
                     /gene="rpmF"
                     /locus_tag="PLW95_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOV21069.1"
                     /translation="MPNPKRKHSKSRTGKRRNSKKIKNVSLSKCPNCGSLITPHSVCP
                     QCGYYKGELVIQKKEKRKKQ"
     gene            16969..17973
                     /gene="plsX"
                     /locus_tag="PLW95_00105"
     CDS             16969..17973
                     /gene="plsX"
                     /locus_tag="PLW95_00105"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOV21070.1"
                     /translation="MKNISIALDVMGGDKVPFSTVKSLECIKKGDNLKIFLVGEKEKF
                     NHLLSSLNPDLYSIVECKKFIPMDKKVGLKLLKETDTTMSVTLRLLKEKKVDVALSGG
                     NTAAFVALAISQIGLIKGVERPAIAVLFPNASKGNTIFLDAGSNINAKPIHFLSSAIM
                     GATYAEIVFGIKKPKVSLLNIGEEETKGDDLRKEAFQLLKNAKEINFIGNIEGQDIFT
                     GKSDVIVTDGFTGNVVLKTSEGISRSFKTLLLRYLRENISGKIATFLIKKNLSQFAKM
                     VDYAEYGGGILLGVDGIVLISHGRSSPRAIFNAMKLGEKIVKSNFLDIMKEKVKDMES
                     "
     gene            17974..18972
                     /locus_tag="PLW95_00110"
     CDS             17974..18972
                     /locus_tag="PLW95_00110"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOV21071.1"
                     /translation="MGVIITGTGSYLPEIVLTNRDMEMMVDTSDEWITTRTGIKERRI
                     APKNISSSDLGKEAILNACNDANIVPEKIDFIICATITPDKFFPSTSCLIEHKLGIKK
                     KFAFDISAACSGFLYGLEIARNLIETSGYQTGAVVAAECMSRITDYTNRETCVLLGDG
                     AGAVIVQRSEENGVIGSYLSSDGGYEYLLHAPAGGSKLPSSFDTIENRLNFMKMEGQP
                     LFKIAIITMCESINNILKKYKLKKEDISLIIPHQANLRIILGVAKNMKIPVEKFFVNI
                     EKYGNMSAACVPVALDHANKKQLIKKGDYILTVSFGGGLTWGANLIKWYKGDKNEK"
     gene            18962..19690
                     /gene="fabG"
                     /locus_tag="PLW95_00115"
     CDS             18962..19690
                     /gene="fabG"
                     /locus_tag="PLW95_00115"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HOV21072.1"
                     /translation="MKNKVVIITGAFGGIGKAVAYKFSNLGAKLVLWDVIIDNEFINQ
                     FTKKETEIIASKIDITNQKEIEEGKNYIIEKFGTIDVLINNAGITKDKIILRMTEEEW
                     DEVININLKGAFLCSKIIGYEMFLKKKGKIINVASIIGQVGNIGQANYAASKGGLIAL
                     TKVCAKEFARSNINVNAIAPGYIMTKMTQNLPEKVKEKMLENIPLRRFGTPEDVAELI
                     AFLSGENSNYITGQVIRIDGGLVM"
     gene            19938..20186
                     /locus_tag="PLW95_00120"
     CDS             19938..20186
                     /locus_tag="PLW95_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOV21073.1"
                     /translation="MEREEIAKIVKDIVVEKLGVDANEVTENASFIDDLGADSLDTVE
                     LVMDFEGKFGLEIPDEDAEKIKTVGDAINYIENNISKK"
     gene            20189..21421
                     /gene="fabF"
                     /locus_tag="PLW95_00125"
     CDS             20189..21421
                     /gene="fabF"
                     /locus_tag="PLW95_00125"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012673575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOV21074.1"
                     /translation="MKKIVVTGLGCVTPVGNNLKTTWENLIKGVSGVDTIKSFDVSNF
                     DTKIGAEVKDFQLDFIHPKEKRKMDKFVQFAIKATDEAIKDSEIELEKEKKEKIGVIV
                     GAGIGGLRVIEQQHEILLSSGPGRVSPFLIPMLIPDMAAGQIAIYFGFKGVNFATVSA
                     CASGAHAIGTALCFMRENNCDVMIAGGTESCITPLGLAGFCSMKALSTRNDQPQKASR
                     PFDKERDGFVMGEGAGIVVLETEEHAKKRGAKIYCELAGYGMSCDAYHMTSPDPTGEG
                     AYLAMKHALVNSNIVPQEVDYINAHGTSTKLNDKSETDGIKRVFGENAYKIYISSIKS
                     MVGHLLGAAGGVEFVITCLTIKEGIIPPTINYEYPDPECDLNYVPNKTIEKDVKVALS
                     NSFGFGGHNITLVAKKYE"
     gene            21432..22589
                     /locus_tag="PLW95_00130"
     CDS             21432..22589
                     /locus_tag="PLW95_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOV21075.1"
                     /translation="MEYFLTDEQQMIKELARKIAEEKILPKRAEYDENEIFPHDIVKV
                     LADSDLFGVFVPENYGGLGYGVFELCLVIEELSRVCGGIALSYAGTALGTFPIVLFGN
                     EEQKNKYLPPIAKGKLAAFAVTESSSGSDITNLNTTAKKDGDWYILNGTKQWITNGGE
                     AETYVVIASTDKSKGARGSTAFIIEKGWDGFEFGKKEKKLGIRASATRELVFTNCKVP
                     KENVLGREGMGFLITIKNFDTSRPGVASQALGIAQGAYESALEYSHQRKQFGQPISSF
                     QAIQHMLADMATQIEAARTLIYQTAKTIDSGAKSYSKESAMCKLFASDVAMKVTTDAI
                     QIFGGYGYMKDYPVEKMFRDAKITQIYEGTNQIQRNVIGIELIKELAKKKK"
     gene            22590..23483
                     /locus_tag="PLW95_00135"
     CDS             22590..23483
                     /locus_tag="PLW95_00135"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOV21076.1"
                     /translation="MKEIVFNQEKNTRIDKYLKEKLNVSRERIKYLIKQEKVIVNSKK
                     IIPSYHLKKGDLISIIQIEEEQKEEIKPEKSNLEIIYEDKNIIVVNKPAGIITHPTNY
                     KKTGTLLNYVLYYTSLAKIGSPLRIGVVHRLDKETSGVIVFAKTSLAYWNLIEQFRNR
                     MVEKIYLAIVKGNFTPEKKVIEFTVLPDKENPTKMKVHFLKGKKTITEINALKYFDDY
                     TLVSVKPITGRTHQIRLTLSYLNYPIIGDEKYGVKSPIISRCALHAWKLTLANPRDNK
                     KQTFIASIPDDIIQIINNSNL"
     gene            23557..24495
                     /locus_tag="PLW95_00140"
     CDS             23557..24495
                     /locus_tag="PLW95_00140"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOV21077.1"
                     /translation="MNNNKIKKLYFPVIFLLTCIFLFLNYKGYKWGEISPGKSVVRLF
                     AVIFSLLSIFHILTQIITFVVKKKKGPLGETKMLVGFSNAIKGFAIIFALIESMGKIT
                     IFGALGVGFFGMLLGWSLQAPVSGIAAWALITLRRPFRIGDRIYLPTLGLVGDVIDIG
                     LMYTILNQVGGAVGSEEATGRDVLIPNAMLFSQVVINYTVKQDQPYFLDEVIWRITFD
                     SDWDEAEKILIEAAKKVTADIIEKTKVEPYIRADVYDYGIYLRLRYITSAIERPKIAY
                     EINKIIFKKVQENPKVDLAIPYTYSYKKGVKGKNNL"
     gene            24566..25828
                     /gene="murA"
                     /locus_tag="PLW95_00145"
     CDS             24566..25828
                     /gene="murA"
                     /locus_tag="PLW95_00145"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOV21078.1"
                     /translation="MEKFVIEGENKLVGEIEISGSKNSALPILSATVLGEGKSIIHNV
                     PDVKDVHTLLDILEYMGAKTKFYNNTVEIDPDGIEKYTAPYELVSTMRGSICLLGPLL
                     SKFRQARFSMPGGCVIGPRPIDLHIKGLKKLGAEISNEEGYIVGKANKLKGNKIFLAG
                     NFGSSVLATANILMASTLAEDETIIEFAACEPEIVDLAMFLKKMGGKICGEGSHTIRI
                     EGVKKLKGTEYSIIPDRIEGGTLLLLGYITKSKIVIKNIIPSHLFALIDKLEESGIEI
                     NIKENIAITKPYNKWKAVDIVTLAYPGFPTDLQAQIMSFLCLADGISIITEKVFPERF
                     IHAGELNRLGADISLDGSKAIVKGVEKLYGAKVMASDLRASVALVLAGLVAEGITEIS
                     RIYHIDRGYEKFEKKLNLLGAKIKREKE"
     gene            25825..26616
                     /gene="dapB"
                     /locus_tag="PLW95_00150"
     CDS             25825..26616
                     /gene="dapB"
                     /locus_tag="PLW95_00150"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOV21079.1"
                     /translation="MSKIKIIVCGARGKMGSLILKLAGEKENFEVTGIVENKNHPDIG
                     EKIDNYILSDNLSNISTGKEIVIDFTVSTATKEFLKVCENKGNPIVIGTTGLSEKQLN
                     SIKNAGKKIPVFFSPNMSLGVNLFLKIIEFSSSLFKNYEVEIKEIHHHFKKDAPSGTA
                     KKIAEIICEKTGRNFKEDIKYGRNGLTGERKFEEIGMHSLRLGDIVGEHYVYWGGKGE
                     IIEFSHRCYNRENFAEGSLIAAEFLSKKEKGFYTMDDLLKEVFNV"
     gene            complement(26635..27288)
                     /gene="phoU"
                     /locus_tag="PLW95_00155"
     CDS             complement(26635..27288)
                     /gene="phoU"
                     /locus_tag="PLW95_00155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOV21080.1"
                     /translation="MIEERMQKLKEKIIDFSLLAEEMVRKSMQGLIERKKEYLSEVID
                     TLENKANMAEVEIDGFCVETIARFQPVAKDLRTIVMILKMNNDLERVGDTAVNISESA
                     LFLIERPFVKKLIDLPKMSEIVSNMLKESVLSFINGDTKLAREICKRDDIVDALNEQI
                     IRELLTYMLQDSSTIERSIHLIRISRSLERIADMSTNFCEDVIFIEEGKIIKHHFEE"
     gene            complement(27302..28051)
                     /gene="pstB"
                     /locus_tag="PLW95_00160"
     CDS             complement(27302..28051)
                     /gene="pstB"
                     /locus_tag="PLW95_00160"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOV21081.1"
                     /translation="MVKIVIKNLNVRYSNLHVLKNVSFDIEKNKVTAIIGPSGCGKST
                     FIKTINRMLDLVDSAKVEGEVLLDGEDIYSMDEVVLRKRVGMVFQLPNPFPKSIFENV
                     AYGLKIHKMFKNKNDLYLKVETALKDAALWDEVKNDLGKNALDLSGGQKQRLCIARTI
                     AIEPEVILFDEPCASLDPIATLKIEELIEKLKEKYTIVIVTHNMQQAARISDYTAFLY
                     LGELIEFGKTDKIFTAPSNKKTEEYLIGKFG"
     gene            complement(28045..28893)
                     /gene="pstA"
                     /locus_tag="PLW95_00165"
     CDS             complement(28045..28893)
                     /gene="pstA"
                     /locus_tag="PLW95_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOV21082.1"
                     /translation="MKKLKQNLWFWVFRICNILILIFFFLIFFFIFIRGFKVINLEFL
                     FSMPKEGMTKGGIFPAILGTIYLVGVSIIFSLILGIGAGIYLSEYARDNYLVRIIKIG
                     INNLAGVPSIIFGLFGFAVFVKLFKFGVSILSGGLTLGILTLPTIIMSTEEALKSVPQ
                     SFREASFSLGARKWQTIKNIVLPNSISGILTGAILSIGRAAGETAPILFTAVTFYINK
                     LPKSIFDEVQALPYHIYGLMTEGTQPEFQVPIAYGTALVLLFLVLGMNSIAIFLRIKT
                     RGKKKW"
     gene            complement(28890..29765)
                     /gene="pstC"
                     /locus_tag="PLW95_00170"
     CDS             complement(28890..29765)
                     /gene="pstC"
                     /locus_tag="PLW95_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOV21083.1"
                     /translation="MTKLKENFIKYILFFLSFLSLFFLLGIVVVLFKEGLPLFKYVSL
                     KDFLFGKEWYPTYEPPSFGALPLILGSLWVTLGALILAIPLGIGASIYLSEIAGRTTK
                     EIIKPIIELLAGIPSVVYGFFGVVILAPFIQKIFDLPVGLCALTASIILGIMAIPTIT
                     SIADDAISSVPNNYKEASLALGATKLETIFKVVLPASSGGIITAIILGISRAIGETMT
                     VLMVAGGAAIIPHSFLEPVRPLTATIAAEMGEAVLGGIHYNALFSLGIVLFVITLFFN
                     ILSDFLYERFKKRVR"
     gene            complement(29857..30666)
                     /locus_tag="PLW95_00175"
     CDS             complement(29857..30666)
                     /locus_tag="PLW95_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOV21084.1"
                     /translation="MKKIFLYLVLTLCLTSFVIAQEKSIILQGSTTVFPVAQRCAEEF
                     IKENPGVSISVRGGGSGVGISALIDKVCDIANSSRPMKKEELKTAISKGIDPKANVIA
                     NDAISVIVNPSNPISDLTLSQIKDIYTGKISNWKELGGENQKIVVVSRDTASGTFECF
                     NEIVLKNEKVKPDALMQASNQAIVNIVKRTEGAIGYVGLGYLGSDVKAVKVNGIYPSK
                     ETVLNGTYPISRVLFMYTNGTPKGLVKNFIDFVKSKKGQEIVKEEGFVPLE"
     gene            complement(30678..32147)
                     /locus_tag="PLW95_00180"
     CDS             complement(30678..32147)
                     /locus_tag="PLW95_00180"
                     /inference="COORDINATES: protein motif:HMM:NF028240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HOV21085.1"
                     /translation="MGRNVIILFFLILLLAFLLKLTISYLSKRKIGTYPEIIKNMLLK
                     LFKSSSIEKNAKKEVKKNKKASREEGLLDKDEYKNKSLEKTTKKGGKMKRKVLLAGIV
                     CFLSFLVSSFASDVDLLIQKLVEKGILTSGEAQTILVETQEEVRKEEAKEREVPKWLD
                     TMKFSGDARLRYEMEDKDPVDRHRGRIRLRYGFETKPNENTEIGVRVATGSSDQKSTN
                     QTLEDVFSSKNIWLDKAYIKYSFNKYISIFGGKFSNPFINTDLVWDGDITPEGIAFQT
                     KFPVGVFINGGFFPLQEDGGWESDPYMTGIQVGYSGKIASRDFKIAVAYYMTEGIEGR
                     TTASVVGAGSAGTGNTIIGGNYIAEYKPIDVVFEFYPFEIQGVPVKLYADYVKNTESE
                     FDKDTGYEIGFTVGKAKKKGTWEFDYNYRDIERDCVPAFLNDSDFNGGGTGSKGHKFG
                     VKYAINDNSTAGITYIVGEGNYVGRGDVNTLQIDYVVNF"
     gene            complement(32132..32473)
                     /locus_tag="PLW95_00185"
     CDS             complement(32132..32473)
                     /locus_tag="PLW95_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21086.1"
                     /translation="MRKIWKVRIKKAYQDATNHIAVGEVLEENQIYIKMRCRTFHFKK
                     PTYSFGIYSSEIKTRIFPWQTISYITELSPDFEWKNVKVELTERGDVVLFTEKEDKKI
                     GIKGGPQWAEM"
     gene            complement(32563..33798)
                     /locus_tag="PLW95_00190"
     CDS             complement(32563..33798)
                     /locus_tag="PLW95_00190"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOV21087.1"
                     /translation="MIKNYFSRLLISNILLLIFFILFNKIYSLPIFICLIFIFLISFL
                     NYLPIKNLKENLRKYKKDVYQKESDIVFIQKTEEEIMEEIEKKEKTIKEMKEKIYSFF
                     KFLPTTIFIIDKENKIVFSNRDEEKFIGKNYLEFFDNYNLFECIKDILNDKEFRQSEI
                     EIGNKIFNCHVFKFFDNTVAVHFLDITETYQLKNIKKELVANISHEIKTPLTVIKGYL
                     ETIYDEVEGDTKENIKIIMKNTDRLINILDDILCLSYIEEKAIQKENVNLKEITDDIV
                     KIFEKKIKEKEIKVKVDMEIKNSLMTDRLLIESILFNLIDNAIKYTDNGEITIKSKVL
                     GNNLFISIKDTGIGIPEKDIERIFERFYVVDKSRSKETGGTGLGLAIVKNAVISLSGK
                     IEVKSQVGSGTEFIINLPI"
     gene            complement(33795..34475)
                     /locus_tag="PLW95_00195"
     CDS             complement(33795..34475)
                     /locus_tag="PLW95_00195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOV21088.1"
                     /translation="MNERIAIVEDEIDILNLMKKYLEKNNFLPFPYTNANDFLKNIEK
                     EKFDLVLLDLMLPDMDGIDVCKNIKTNPKTEKIPVIMLTARTDEGDKILGLEIGADDY
                     ITKPFSLKELLARIRAVLRRYKLELSTEKIYIGKEIIIDPKTFEVLVNNKRINLTYVE
                     FKILLILASKKSWVFPRDKIIELLWDGEKFTIEKAIDVHINNLRAKLGKYGKYIKTIR
                     GVGYKIEE"
     gene            34684..35841
                     /locus_tag="PLW95_00200"
     CDS             34684..35841
                     /locus_tag="PLW95_00200"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010877916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HOV21089.1"
                     /translation="MFDISERMKKIPPYLFVEIDKKKKKLIKDGYDLIDFGIGDPDIP
                     TPSNIVSAMAEYIKKPEFHRYPLGRGNINFRKSISSFYQENYDVQLDPEEEICVLIGS
                     KEGIAHFPLCFINPDDYSLIPEPGYPVYKIGTILADGNCYFLPLKEENNFLPDFSKIP
                     ANILKKAKILYLNYPNNPTSEFASAEFLKEAINFCKKYNLILVYDAAYSEIYYDERPV
                     SFLSLPGAKDVGIEFNSLSKTFNMTGWRIGWACGNKELISALSKVKENIDSGVFEAIQ
                     FAGIEALTNSKETPLKMRDIYKKRMNLFVNGLKEVGFDVKFPKGTFYLWLKVNKSSVE
                     FVDELLENGKIIATPGIGFGPSGENFVRFSLTIQENKIIEAIERIKKIWEK"
     gene            35829..36281
                     /gene="purE"
                     /locus_tag="PLW95_00205"
     CDS             35829..36281
                     /gene="purE"
                     /locus_tag="PLW95_00205"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOV21090.1"
                     /translation="MGKVAIVVGSKNDLELIEGAKEILKNFEVEFDVFVLSAHRTLDD
                     TISFAENAEKKGYEVIIAAAGMSAHLPGIISAKTILPVIGVPLPTSEIKGMDALLSIV
                     QMPTGVPVATMSIGKAGVKNAALFSIEILARNDEKLKEKLIEYKKNLK"
     gene            36370..37344
                     /locus_tag="PLW95_00210"
     CDS             36370..37344
                     /locus_tag="PLW95_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HOV21091.1"
                     /translation="MAVSYKELGLVNTQELFKKALAGGYAVPAYNFNNMEQLQAIITA
                     CAECNSPVILQISKGARQYANQTLLRYMVPGAVAMAREMGSNIPIALNLDHGDSFELC
                     KSCIDYGFSNVMIDGSALPYEENVSITKKVVEYAHERDVTVEGELGVLAGIEEHVSSE
                     VSHYTNPKDVEDFVKRTGVDSLAISIGTSHGAYKFKVKPGEPLPPLRFDILEECAKRL
                     PSFPIVLHGASSVLKEYVDIINRYGGKLENTAGVPEDQIRKAVTMNVCKVNIDSDGRL
                     VMTAMIRKYLAEHPEEFDPRKYLGPAREELKKMYIAKCNLLGSAGKGQ"
     gene            37389..38354
                     /gene="pfkA"
                     /locus_tag="PLW95_00215"
     CDS             37389..38354
                     /gene="pfkA"
                     /locus_tag="PLW95_00215"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOV21092.1"
                     /translation="MKKIAVVTTGGDAPGMNPAIRSVVRTALYKNISISGIRRGFLGL
                     YEGDFIPLSSRDVGNIINRGGTILKTARFPEFKDHNIRKKCLEKLKKEKIDGIVIIGG
                     DGSANAAYSIYNEFSYPVVHIPASIDNDLYGTDWTIGFDTAVNTAVEAIDKIRDTATS
                     HERTFIVEVMGREKGNLAVEVAVACGAENVIIPEIEFNLDQLVSSLKQQQEKGKNSAL
                     IILAEGAGKAEELAKQLTEKLPDREIRYSVLGYIQRGGSPTYLTRTLATVFGWYAVKF
                     LIDGDYGYMVGIVGNKIEKVPLEKVVSNRKLPDMEKLETIKYMAI"
     gene            38447..38638
                     /locus_tag="PLW95_00220"
     CDS             38447..38638
                     /locus_tag="PLW95_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21093.1"
                     /translation="MSKEFCREEKKKIKELFKAKKLFHKEMAKIPFEEKIKIVVRLQN
                     IVNELKTNKGRRKKRVWKI"
     gene            38655..40304
                     /gene="ilvD"
                     /locus_tag="PLW95_00225"
     CDS             38655..40304
                     /gene="ilvD"
                     /locus_tag="PLW95_00225"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOV21094.1"
                     /translation="MRSDNIKKGIEKTPHRSLLKADGFTDEEIKKPIIGIANSANEII
                     PGHIHLNKIAESVKTGIVSAGGTPVEFGVIGVCDGIAMGHLGMKYSLVSREVIADSVE
                     IMAQAHQFDGIVLVASCDKIVPGMLMALLRLNIPGILITGGPMLCGYLPTGETIDFIS
                     VSEGVGKFLKGEIDEKQLKILEDEGCPGAGSCAGMFTANSMGCLSEGLGLSLPGNGTI
                     PAVSSKRIRLAKYAGIRAVELVKKDIKPSDIISIESFKNAITLDMAIGASTNTVLHLP
                     AIANEIGIKLNLEIFDEISKKTPNICKLSPASSQHIQDLDRAGGIPAVMKELSKNGLI
                     EKNCLTVSGKTIGEIIEKANVWDRNVIRDINNPYLQEGGIAILKGTLAPDGAVIKQSA
                     VKENLLKFKGNAIVFDSEEEAMKSLVEGKIKEGVVIIRYEGPKGGPGMREMLSMTAVI
                     AGKGIDEKIALITDGRFSGGTRGLCIGHVSPEAEEDGPIGYVENGDIIEIDVPKRRLD
                     IMVKPEELKNRKRKKKEKKLKGILARYSKQVRSANTGAILE"
     gene            40348..41631
                     /locus_tag="PLW95_00230"
     CDS             40348..41631
                     /locus_tag="PLW95_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013918434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOV21095.1"
                     /translation="MEDIRKGKGDYEWFIKARFGLFIHWGLYSMPARREWVRSYEEIP
                     NEIYDEKYFKRFYPDLYNPKEWAKIAKNAGMKYAVITTKHHEGFCLWDSKLTDYKATN
                     TPIKKDLLREFVNAFREEGIRIGFYYSLIDWHHPHYTIDMYHPLKNHPEKEKLNKERD
                     MSIYRKYLHGQIKELLTEYGKIDLLFCDFSFPGPDGKGKDDWDSENLVKMIRELQPHI
                     LINDRLDLPDAWDFKTPEQAVPYEWPEVNGKKVIWESCQTFSSISWGYNRDDIWRDSE
                     EITKTLIDCVSKGGNLLLNVGPTARGDFDYKSRKRLEEIGNWMKYNSRAIYNCTQAPK
                     EFNIPKDCRLTYNPEKGTLYIHLFSYPYKFLFLEGKEWKENGSYAQLLNDGSEIKIGL
                     DEWYKNQLGDKEGIVLCLPPIKPNVSVPVIELFLK"
     gene            41656..42936
                     /gene="thiC"
                     /locus_tag="PLW95_00235"
     CDS             41656..42936
                     /gene="thiC"
                     /locus_tag="PLW95_00235"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013755865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOV21096.1"
                     /translation="MEKELAKKGIISKGVKFISEKENVNPEIIAKNIAEGTCVILKHK
                     DTYLGIGKDLKTKINTNIGTSPDIFDLNLEIQKLKYAIKYGTDTLMDLSTGGDLNKIR
                     KEIIKESSVPIGTVPIYQSAIEVAEEKGGITKMDADKIFQIIEQHAKDGVSFMTVHCG
                     ITLNTLQRLKKNPRKTLVVSRGGAFLISWMISNEKENPLYKNFDRLLEIAKKYDVVLS
                     LGDAMRPGGIIDSTDIAQIDELLNLGELTRYAEEENVQVIIEGPGHIPIDEIETNVKL
                     EKKICKGAPFYVLGPIVTDIAPGYDHITGAIGASIAGWCGADFLCYVTSAEHICLPGL
                     DEVIDGVISSKIAAHVADIGKGIKGTKEIDYKMDMYRTDRNWEKEKEISFFPDKFEQL
                     HKKYEKTERDVCTMCGKYCAIKLVEQYLGSKIIF"
     gene            42938..43426
                     /gene="def"
                     /locus_tag="PLW95_00240"
     CDS             42938..43426
                     /gene="def"
                     /locus_tag="PLW95_00240"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOV21097.1"
                     /translation="MRKIRKYGEEILRKKSKEVEKIDTNLLKLIESMKQTLVYYKGLG
                     LAACQVGVLKRVIVALDQKTKEIITVINPEIVETSGQDIDMEGCLSFPEIYFSIKRPK
                     KVTLKGINEKGKEFIYEGSGVLGRCFCHEVDHLNSILIIDYATEEERNYWKEKIDKIK
                     NK"
     gene            43600..45189
                     /gene="nadB"
                     /locus_tag="PLW95_00245"
     CDS             43600..45189
                     /gene="nadB"
                     /locus_tag="PLW95_00245"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOV21098.1"
                     /translation="MIPDYLVSFDTEKLSIEEVDFLIIGGGVAGLRATLEIKNYKTLI
                     VFKNGIEESSSFNAQGGIASAIAPGDKDEYHINDTLKTGCGIADKQAVEVLVKEGKER
                     IKELIEMGFKFDKINGKFHFTLEGGHSVPRILHSNGDFTGQTLTSFLYEKVLSMPNIK
                     IIHSFFLIDLIVEENKVYGGIFLNTENKKLFIIKAKAVILATGGAGNVYQETTNPSSI
                     TGDGIAIAYRSGAVLSDMEFVQFHPTTLYIAGAPRFLISESVRGEGGILRNINGERFM
                     FKYDKNGELAPRDIVSRAIIDEIKRTSSSCVYLDITHLKKSYIKKRFPHIFKFCLSYG
                     LDITKNLIPVRPSAHYFIGGIKTNLWGQTNIKNFFACGETASTGVHGANRLASNSLLE
                     GLVFGARCGKKIIEDFSNIKIQNIKQKYKFPQKGDILIDRYDLKRSIKSLMWRQVGIE
                     RNGDKLKDANNRLKEWEKYAFLKEFYDKDGFEAQNMLIVSKLIAHSSLKRKESRGTHF
                     RFDFPCTDDRWEKHILIKKNK"
     gene            45415..46803
                     /gene="gltA"
                     /locus_tag="PLW95_00250"
     CDS             45415..46803
                     /gene="gltA"
                     /locus_tag="PLW95_00250"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOV21099.1"
                     /translation="MIKDRIKEKKQKPEERKRNFNEVSFGYTKEEAVEESKRCLQCPK
                     GPCVQGCPVGINIPAFIKELQEGNIQKALDIIYEKNLLPAICGRVCPQETQCELFCSL
                     NKAKKPVAIGKLERFAADYGQQIYKCNTEKISEIDKNKIAVIGSGPAGLTCAAELAKC
                     GIKVTIFEGLHLPGGVLSYGIPEFRLPKSIVFKEIEFLKKIGVEIKTDIIVGKTITIE
                     DIFNDEYRAIFIGSGAGLPSFMGISGENLLGIYSANEFLTRVNLMKAYNFPEYDTPVF
                     IGKRVAVIGGGNVAMDSARSSLRLGANEVFILYRRTEKEMPARKEEYENAVEEGIKFT
                     FLVQPLEIIGDENGHVKGIKVIHNELGKPDESGRRRPVPIEGTEEILSFDTIIVAIGQ
                     NPSPLIPKSYEKLKTGKKGNIEVNPETCETNIPGIFAGGDIATGAATVISAMGMGKTA
                     AKNIIERIKKGQ"
     gene            46805..47386
                     /locus_tag="PLW95_00255"
     CDS             46805..47386
                     /locus_tag="PLW95_00255"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOV21100.1"
                     /translation="MKIKMSICKWTYRNRGILVSPPLLFAIFSFFDEIEVDYLIWPLG
                     ISIFLLGLLLRIWAQEHIHYRLKTEGHLTTTGPYTSIRNPIYLGNTLICVSATILSEL
                     LWLVPITIIWCGIVYSLVVRYEEENLLELYGEQYHKYMSEVPRWFPHSFKNAKNLMFK
                     NEYLSASLIAEIQCLFALLPPILKEIISFWINH"
     gene            47490..48233
                     /locus_tag="PLW95_00260"
     CDS             47490..48233
                     /locus_tag="PLW95_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HOV21101.1"
                     /translation="MMGWIILGVFVFILLANSIKVVNEYERGVIFRLGRLVGARGPGL
                     FFLIPIIERMVKISLRIITFDVTPQEVMTKDNVPIKVNAVVYFRVMDPNKAIVEVENY
                     QMATMQIAQTTLRGIVGQHELDEILAEREKINQRLQQIIDEQTDPWGIKVSIVEIKEV
                     ELPDTMKRAMARQAEVERERRARVINAEGELQASEKLKQAAEVLSQSPVAVQLRFFQT
                     ASEIAAEKNSTLVFPIPLEFLKYLEKKNE"
     gene            48226..49086
                     /locus_tag="PLW95_00265"
     CDS             48226..49086
                     /locus_tag="PLW95_00265"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HOV21102.1"
                     /translation="MNDFKKIGIVGLGLIGGSIGKTIKKKSSKITVYGISRRQATLIK
                     AKEENIIDEYFLNFDEIVENSELLVLCTPIDKIEYYFRKIKQTGKNIYLTDVASVKKR
                     IVEKGEKILGPLFPYVGSHPMAGSEETGLFASSDNLFEGKNVIITPTKSSDKNVVKKI
                     ENFWQFLGANTFFLTPSEHDKFVSLTSHLPHFLVFQFLKIISQEKEKKKIEKCIGPGF
                     LDSTRIGKSNVEMWTEIFLNNKENILRGISKLQKEIEKTKKYLKANNKEKILKEFEKG
                     NKWRNSIEKK"
     gene            49073..49867
                     /gene="proB"
                     /locus_tag="PLW95_00270"
     CDS             49073..49867
                     /gene="proB"
                     /locus_tag="PLW95_00270"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOV21103.1"
                     /translation="MKKNDLIKKAKRIVIKIGTNVLTTQTNRLDTSIIEHLVEQITYL
                     IEKKEKEIIIVTSGAILSGMQVLNWEEKPKQINELQAVASIGQGKLMGAYERIFKEEG
                     INVGQILLTRDIFMSKKRAKIARETILTLLKHKIVPIINENDSVAFEDIKFGDNDILS
                     AYVTNLIEADILILITDVDGLYKNYSDKKGGVIREVKDIEKLENISFLGKTSRKGTGG
                     MKSKIQAANIVTKNGKPCLIINGKKMWTLKKVFEGKEIGTFFYPAR"
     gene            49873..51129
                     /locus_tag="PLW95_00275"
     CDS             49873..51129
                     /locus_tag="PLW95_00275"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOV21104.1"
                     /translation="MNWKDDIKIKLEKTKNAYKSITGLTTEEKNKFLENLAQNIKKES
                     PNILKSNKKDVEKAQMSGYSNAFIDRLSLSENNIEKIISSIENVRNLPDPVGKIIWEN
                     KRPNGLIIKRKRVPIGVIGIIYESRPDVTVESSILCIKAGNCVILKGGKEAKNSNKKL
                     VEIIKKSLKEASLPEDCVNLIYKGGRKSVKHLLKFHQYIDLIIPRGGEELIKTVVENS
                     SIPVIKHYKGICHTYIDKYTNIDMALKVTFNAKVQRPGTCNATETLLVHKDIAKEFLP
                     EMAKMFEEAGVEMRGCEETRKILPFIKKASEDDWKIEYLDKIISIKVVNSIEEAIEHI
                     GKYGTSHSDAIITENKEMAEKFLNMVDSAAVYHNASTRFTDGGEFGLGAEIGISTDKI
                     HARGPMGLEELTIYKYIVYGNGQIRE"
     gene            51110..51703
                     /gene="nadD"
                     /locus_tag="PLW95_00280"
     CDS             51110..51703
                     /gene="nadD"
                     /locus_tag="PLW95_00280"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007555391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOV21105.1"
                     /translation="MDRLENKTIGLLGGTFDPIHIGHLIIAEKVREEFNLEKVIFIPA
                     GIPPHKKSAFTSSFHRYNMVKIAVSDNSFFDVSDIEIKRNLPSYTYLTIKKMKEIYKN
                     YKIFFIIGQDSLLDLPNWYKADKLIKETTFIIVRRNTDFYYEKDKNFPVNFELSNNPI
                     IGISSTYIRTCIKERKSIKYLLPEKVKNYIEKENIYG"
     gene            51696..52721
                     /gene="pheS"
                     /locus_tag="PLW95_00285"
     CDS             51696..52721
                     /gene="pheS"
                     /locus_tag="PLW95_00285"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOV21106.1"
                     /translation="MDKEEIIKKCRDVFNRGKEELEKLIDENDFEKWRTKFFGRKGEI
                     NKLFSFLSTIKNELKPEIGEEINKIKINLMSLFEEKKEIIQKERNKEKFNLSFPGKIP
                     EYGGIHPLTQIMKKMTKIFENMGFGIFTGPEIETDFYNFEALNTPEDHPARDIWDTFY
                     LDYKKKILLRTHTSPVQIRVMETNKPPFRIIALGKCFRRDQFDSSHSPAFHQMEGLMV
                     DKNISFTHLKGILNYFLKEMFGENIKVKFTPSYFPFTEPSAEVSISCVICGGKGCSTC
                     KNSGFLEILGCGMVHPQVFRNVGYNPEEITGFAFGMGVERIAMLKYKIDDIRLFYNND
                     LRFLKQF"
     gene            52723..54714
                     /gene="pheT"
                     /locus_tag="PLW95_00290"
     CDS             52723..54714
                     /gene="pheT"
                     /locus_tag="PLW95_00290"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOV21107.1"
                     /translation="MRYSFKLLSEYIDGEIKFKQLIEFIDILGLNPYIIEKNNDIIFE
                     LEIPANRGDLFSLIGIIKEIIPFTENKLLLPEIKFSEEIDDIKRIEIENINDCSYYSS
                     RIIKNLENISSPSEFIEKIKKLGFKSSFLPVDISNFVMAETGQPIHIFDLDTLEGNII
                     IRRGKENEKITCLDGNEYKINPDILVISDSKKAVAIAGIMGGINSCVTEKTKNILIES
                     AIFNPIVIRRGSKKLGLITEASARFEKGINVEITKFGMERTAYLIKKMCGGKVGKVNY
                     SGKKESSEIEIKLRKERVDKIIGVEVEEKFIEEHFKKVNFDYTKQNNIFTVKIPPYRK
                     DIKEEIDLIEEIAKYKGYSSIKEETPYTNITPTTSDETYQKYETIKDIIVKFGFNEII
                     TLSIISDDFKNIFGEEYVRIENPLSSNFAFLRNYLFPQMLDTFKYNINHQIKHLKLFE
                     LGKVYFKENEKFKEETHISIGILNSGDYYDMKGVVEEFLRYSNIENLNYKLDKNFISE
                     ENGLQSIFYKEKKIGEIFIPKREILEKFEIEQEQIYGAEIYIEKIINEITFEKKFNYL
                     PKFPFSKRDFSFILPFEIDWGKIEKVILLENIPIEKINVFDIYKGENVPAGKISISFS
                     IYFRSDEKTLIAEEIDEFSKKIISIIEEKFAGELRGEKK"
     gene            54701..55009
                     /locus_tag="PLW95_00295"
     CDS             54701..55009
                     /locus_tag="PLW95_00295"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOV21108.1"
                     /translation="MKKNKIRILGKEYLFQSDFDEKQLKESEKKIEERIKHYESQYPN
                     ADKIDLLVVFILELLENIYIKEKQICEREKKFENLKGKLEMMEKNIKEKLNILTKEIQ
                     "
     gene            55039..55110
                     /locus_tag="PLW95_00300"
     tRNA            55039..55110
                     /locus_tag="PLW95_00300"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55071..55073,aa:Met,seq:cat)
     gene            55261..56976
                     /gene="pilM"
                     /locus_tag="PLW95_00305"
     CDS             55261..56976
                     /gene="pilM"
                     /locus_tag="PLW95_00305"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HOV21109.1"
                     /translation="MLGKGRKFAVDFGTTKIRIADFENRGNKIYCNFYQEIPIPYYSP
                     EERENFLKKEAKGILDKNKIKTVIVSLPGRGLLLRQLNIPKVPPKKLKDILKYEVQQQ
                     IPFPLEVVEWKYQILGEEGVNLNILLSAIKKELVNEYIGNITGFGIDPVFLDTDLFAV
                     YNAFRYSPFYNEDKCTAILEIGETSSNFIILYRKMVLMRSLTISGSTITSSIMETEGI
                     SYKEAENKKIEMGMQLPACISSIESLNTEIQNSVDYWRFTQKGPEVSELYICGKTSLL
                     NGFKESIQEKSKIKTEYFSPLSTIEINPKYENLRENEVELAVLTGIALRNAGISFINI
                     DMLPEEIKRVREFKLNRLYIYVSIILAGLISITPVLFLNQDKAMLKGILSDIEISLSQ
                     YEKYEKDVNKLQNEIKETKEKCETISGLISKKGTWLKNLIYIGRCLPSSKIYLTSFSP
                     GAESQTQQGEQAPISPEGQPVMPPGGPSMPPAGAPMPPGEPSIPQSPQEQIKPNEVNI
                     ETQNVYTLKGEVVITDIKSAFDNFKSFVKNISNLEFIQKVDISYCEVNSERNVIEFTL
                     LLNIK"
     gene            56973..57752
                     /locus_tag="PLW95_00310"
     CDS             56973..57752
                     /locus_tag="PLW95_00310"
                     /inference="COORDINATES: protein motif:HMM:NF038287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Amuc_1100 family pilus-like protein"
                     /protein_id="HOV21110.1"
                     /translation="MKKILGLLKKYSLIFSLITVVGIIGVGYYFYNKSQKEIEKIENE
                     IREKYTKAKEYEKAGENAPSPELIGKLTKEKEKYEGNFKYMVNNFSTEQPKVPTFDIY
                     PSIEFKEYILFSENKLYKKAKRMEVNLPSSLGFPKKGLVSPDQIPIFTLQFEVVNNLI
                     NLVINSGITSITSITPGTSRKVSFYEMMPIKLVLTGTSNEIIRCLKYFDNPSSYFLVE
                     NFLVTKLNENLFKVEINLNAVMLLVGEQKLAGKNEKENPSG"
     gene            57727..>58241
                     /locus_tag="PLW95_00315"
     CDS             57727..>58241
                     /locus_tag="PLW95_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21111.1"
                     /translation="MKKKIHQDNIREKNREKLLNFILEKKKKRDKEDIPDDKRRNVSL
                     LKEQKKKEKEERYLSIQQRRDKEKLEKIKEREKRKAEKEEERKKRLLIKKQKLEEKQK
                     KREEIEKQKQIIQQKKEEREKKLQEKEIAKQKKLEERKKQQQEIEQKKQQLLEEKQKK
                     REEIEKQKQII"
     assembly_gap    58242..58341
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <58342..59765
                     /locus_tag="PLW95_00320"
     CDS             <58342..59765
                     /locus_tag="PLW95_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21112.1"
                     /translation="KQKKLEEREKQQKEIEQKQQLKKQTLLSLINKIKSENERKSLVI
                     KAEKVENRKKKILLKKQKLEEKQKKREEIEKQKQIIQQKKEEREKKLQEKEIAKQKKL
                     EEREKQQKEIEQKKQQLQLLKQQREQQIKTQREEKEKIKQEKEEERRKKIEIEKRKNE
                     RRKILIALTPIINKATAIDKETKKQLNLIQQKISKLTKKVEIQKKKIPEKSKIVPVEK
                     GKIVKKTVKKEIQPVIYKEPFNLGDFLRKNAFKFIFLLLLIAWFIELFLFTSRLKSPE
                     SRLEMIVGKTIEEKREQIQKKSPETISTENFVVKKEEINIEGERDPFSPGKLTMAVMK
                     KLQPTNIVLTKEPEVITIVKKPKVVSILKEPTLMQSEQVSSIVKPTKPSSVSSSKEEL
                     STIEKITKPSISPIIIQKEKCPLTYRGRMLFNGVEYFFIEGEKRTYRVTIGDIVEGFK
                     ILKKEEDKLFLSKNGEIYEINVF"
     gene            59807..61672
                     /locus_tag="PLW95_00325"
     CDS             59807..61672
                     /locus_tag="PLW95_00325"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOV21113.1"
                     /translation="MKKIIKIGICIIFIITSFSYAEVDKKQLEEKYRKGISFYYDKDY
                     AQALVIFKEIQGEDPTYRASQIKRYITRAQTELGKLGVKEKFVAGESEIKEVKIKKEG
                     ELEALSAAAQKTLLDTYQFFSEEKKKYNVSDFDMTGPESTLKLAKEAYDNKEYTEAIR
                     LANKSRLELENIVQKKVEEKKVVSLGEIGETPVTLNLTNADLEQTLKLIYDLTGANII
                     LSKGISGRVTINVKDLPLKKVLNLICEANQLKYLEEDGIIKIMTQEEFNKSAEVIKQQ
                     NRRVFKILYGDASSIAKSIRDTYKIDRVIADPRTNSILLDIDDPIQLARIQETISMLD
                     APISQVLIEAKIIEIRTSTDNMLSIDWLVSSRLVDELNATLTGPKFGDNPAFTPGASS
                     TLPGGFSFGLTNADVNTLISTLATHGDVKLIQAPKITCLNGTNAIISVIQNYPYIIPE
                     YSETYNEQGTRTGTRQTVTVYEETVGTEFEVTPIIQTDRSVFLNLNIYDSRLVEIRKL
                     TAIAAGQRYETEQPIVSSRETNQSVTLYDGQTLVIGGLIQSAEEKNESGIPFLRRIPV
                     LGYLFKKPKYTNTKSELLIFLTPHVITTFKEVEAVTGPDIKKGEKEIEPGLLKHF"
     gene            61731..62711
                     /locus_tag="PLW95_00330"
     CDS             61731..62711
                     /locus_tag="PLW95_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOV21114.1"
                     /translation="MKNKISLLLILSFLGFMILPSFSQTSSSKSSSSRTTRKDFRGDT
                     DDKNEERQGYIRDFVVAVIGTKLRHPDPQIRMQALQSILTGILSGEGEGGGDENEGLK
                     SYFSIGTENSSGDTGDYVGAGAAAFIPDIYSLLKDPDPEIRDVAGVGLDALFGSDVTL
                     LRFMDDPDPVVRKYATKIYIKKALVASQDDDNNNDNENKTGDLAELLALRTLLVRLKY
                     ETDEGVRKTIEDSIEYYLTSETGRDRGDERGYVRGVDSSIIKYLGDSNPEIRKNAAKI
                     IGELEYNPSILNILMEALRKENNSEVKEEIQASIEKLTANLSQVQKKELR"
     gene            62732..64321
                     /locus_tag="PLW95_00335"
     CDS             62732..64321
                     /locus_tag="PLW95_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOV21115.1"
                     /translation="MKKIFLLFIFILSSFFVCADNFVLNFNNVDIQAFIKFVGEFVQE
                     NYVIDPTVKGNVTIYSNKPVPVDQIDQIFKTVLSLYGFTVIKKGDISFIIPVSEGKAR
                     TGIINIGEVPDDKMGDYIIQIIPLEYYPAQTISQILNPYISKGGQITVDTRTNSLIVS
                     DYGENVKKIFEIVKKIDTPAPKGKEEFRIYRLENADSEEIAKILTQILSQKTRVVTRV
                     QTAEVPIQPSVVSAKSTNSLIVHAEPEDFDEIEKIIKSLDVLTNQVLIEALIAEVTYD
                     KAQDIGVEWVAPEAFEVDKYGIAVEGNVGTLQNIETGGLSIGIYKGSYGYPLNIGALI
                     RLYGKDSHFNILSAPQIMTTDNQEATINISENIPYLKETRFVESTSGSTGDTIKSYDY
                     KDVGITLKITPQISQNKYVKLKISQEVTKLIEGGTPEAPATAKRSADTTLIVANNKTV
                     VLGGLIRNDTENSVIKTPFLGDIPLLGKLFRKESEKSTKTNLLIFITPRIISSFEEAE
                     AIKQEKEKIFPKGPDNKNGKN"
     gene            64308..65825
                     /gene="gspE"
                     /locus_tag="PLW95_00340"
     CDS             64308..65825
                     /gene="gspE"
                     /locus_tag="PLW95_00340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HOV21116.1"
                     /translation="MEKIENLEEKIEPEVLNLFPYTFLKKNCFLPLNGENGEIIIVTD
                     NLLKIDIEEIKNIVKKEISVVIMPTEKIEECIEKFYYHPTSSKRVIDDLKEEEDITEL
                     KDEDISGTTNLLDLANKAPVIRIVNQIIYRAISTRASDIHIQATESEVKIRYRIDGVL
                     HDIFVLPKKFHPAIISRIKIISNLDIAEKRIPQDGRTSIKVDSKKIDIRISIIPTFFG
                     ESAVLRLLDRNSYLFTLEQLGFSEENYKKFNRLIYCDHGIILLTGPTGSGKTTTLYGA
                     LSKINNPGINIITLEDPVEYNLEGINQIHVNPKVGLTFANGLRAILRHDPNIIMVGEI
                     RDVETAEMAIQASLTGHLVFSTLHTNDSASAIVRLIDMGIEPYLIASSLIGVMAQRLV
                     RVNCPHCSQSITPSLELLKEISLGEQDLKEGNFKEGKGCKKCYNEGYYGRTAIFELLA
                     IDDTIRNFILERDSSQKIKNYSIKKGMKTLRMDGAEKVKQGITTISEVLRVTQEF"
     gene            65825..67018
                     /locus_tag="PLW95_00345"
     CDS             65825..67018
                     /locus_tag="PLW95_00345"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOV21117.1"
                     /translation="MENFQYIALDKKGKTISGKIEGNDVDDIKRNLKEKGLYVIKVEK
                     LKEKFPFLKKISEEELIISIRELATFINSGLQIDEALTGLISQMKEGNLKNIYKEIQK
                     NIREGQSLSMSLKKYPFIFSDTIISMVKAGEETGTLDIVLTKISEFLEKNHRFKTKIM
                     SIMSYPILMLSVASVVVIFILSFVIPTITKVFSQISMELPIMTSILINISSFLKKWWY
                     FVFAGIALTLWGINVFLKTSRGIKFSDNLKLKTPFFKDIYIKKEIVNFSRTIATLIKG
                     GVDIVDGLVISRNVLTSPSIKNELNNIIELVTKGGTLSNAFSNSSYFPYLVTQLVNAG
                     EKSGNLSEMFDKIADIYEEEINQKSTKFVNLLEPGMIVFMGIIVGFIVLAVLLPIFQI
                     SQSIK"
     gene            67187..67624
                     /gene="gspG"
                     /locus_tag="PLW95_00350"
     CDS             67187..67624
                     /gene="gspG"
                     /locus_tag="PLW95_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006964725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HOV21118.1"
                     /translation="MNNIRKSGFTFIELMVVVVILGILAMSVMPRFFERIDEAKITAA
                     KVQIQNFESGLKLFYLDNSFYPGTEQGLEALIEKPTTGKVPQQWREGGYLEKKVIPKD
                     PWGNPYVYLSPGRNGEDYEIISYGRDGKEGGEGPDADISSSQL"
     gene            67736..68122
                     /locus_tag="PLW95_00355"
     CDS             67736..68122
                     /locus_tag="PLW95_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21119.1"
                     /translation="MEEISNFISNIKKESIKNQQILYFCIDIDINEFWIEQEKKEKNK
                     EEEIGEEEEIEKYQTSFDFILCKNTDEEKGNGIMKFKIFPDGYVQPGFIYINDPETNI
                     IWTIFINPYLNIPEIYEGEVTFEEYQ"
     gene            68106..68495
                     /locus_tag="PLW95_00360"
     CDS             68106..68495
                     /locus_tag="PLW95_00360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOV21120.1"
                     /translation="MKNINKNGFTLLECIIAIGIFGIIAVVCFEMYFMEIKKVKILSE
                     KQVLLQLAQSKAEQLLIENEEIKEENGEFESPFSDYTWEIKLSDISYSDTENDFEITP
                     YILIVKYQNNFISYFIPFAKEKKENEK"
     gene            68485..69021
                     /locus_tag="PLW95_00365"
     CDS             68485..69021
                     /locus_tag="PLW95_00365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOV21121.1"
                     /translation="MKNNGFTLIEILISTVIIFITVSLIYLTYYNLEKETLVIEEKLE
                     AIEIKFNFLTSLKEQLKSAIEKKDTFQFSSDSILFEATSNYSPYTFQYKFYTYQTDKG
                     FDLIEERTNLFTQKKIIIPIAKNYYSFNFEFYDGNNYFDNWDKEKMPVGIRIKISEGE
                     NEEFYYYIKLPTKDEKKE"
     gene            69005..69979
                     /locus_tag="PLW95_00370"
     CDS             69005..69979
                     /locus_tag="PLW95_00370"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HOV21122.1"
                     /translation="MKKKNKEGIILIYVLGFTLAITTLLLVVHYKVEQFMDEFTNNTE
                     FIQMENLAEVGYSIGTNLLESDQNSYDWYGESWRKDIEIDTEKYKIKVKITDEDGKIN
                     INKVIGEKGEINTLLLQILKSVFTISGYSDSMVDCLLDWIDEDELPRSSGAESTFYKM
                     SGYPYVPPNRTLYSIEEITLIKDFNKEIIYGKSKEQNEEDKETNNEEEMKKGLINFIS
                     TISDDKINVNTCEWEILNSMGYTEVNIDQIMDQRDRGPLSENYLLEINREVTLNNKRV
                     IKYKSSNFSMNVSVENIENKKIRNFKFFVFRDEKRGMKLIRKEKIWEN"
     gene            69967..71211
                     /locus_tag="PLW95_00375"
     CDS             69967..71211
                     /locus_tag="PLW95_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21123.1"
                     /translation="MGKLNIYIRDNNSTYYYKNKEEKREFIEEVNIKNIIEKEKNNYI
                     NFILSKPDVIFRNFSFEFSDIKKIKMVLPSELEESLSEKIDNFLFNYRFFKQGKKTLV
                     NVTGVKKEFYNNLISLIKNKQKVSFFTDVEILFYSLKNFIKDENYIEIYIDKFYILIN
                     VVENRVISSSISYIFENTSSEKIISVVGNTLKEKAEKIYITGEEKEIENFANKTGLKI
                     NKVGILKQYQEFYFTEIINYKSIPTLNFSSSEIKIITLTPFLIPFLIFILLLIIPLTL
                     FNNLKQKQNELNQIEEKMINEFRKTFPDVKKVVDPVIQTKEKLNETGKTSFSFQYSSI
                     LQLLIEVTKIFPENVRIEIDQLSYLKNVLTISGFIDNLNNLEKVKKEINKSSYFESYE
                     IGTISFDKNNRINFSLTLKVKK"
     gene            71208..71705
                     /locus_tag="PLW95_00380"
     CDS             71208..71705
                     /locus_tag="PLW95_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21124.1"
                     /translation="MNKKLITLIVIALFVFEYQFIITPLNKKIRSTANLINKKKNDYE
                     TLKNLIEEYNEKNRNKSIENAKIVNKNFSLFSFVGKIIEENALVSNIKSINPLPVEKK
                     DNFEIEKIKLNIDNIPLEKLLKFLANIEESNFPVYISEFSMVRNKTNPSLLIVNMEII
                     VIKNG"
     gene            71698..74103
                     /locus_tag="PLW95_00385"
     CDS             71698..74103
                     /locus_tag="PLW95_00385"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOV21125.1"
                     /translation="MAEIYLIDGSNILYRSYYAIGKLETSTGLQTNAILGFFNTLFKL
                     LKKHKIEYIGIFFDMRKPTIRHIKFHDYKIKRKPMPDELEFQIPIIKEILNCLGMKYF
                     EKEGYEADDIIATFTEKFKKDHKILIVTGDKDILQLIEENVNIISPVNEEIKDINYIK
                     KNFNFAPEKIIDFLALAGDTSDNIPGIPGIGEKIALKLLLQFSSLEDIYDNIEKIEPE
                     SLREKIIKNKEMAFLSKELVTLHKDIDIKVNLDDLKVISPDINKLEEIFTSLEFKKLK
                     EKLYQIFPEIKENNPKEIIAFSNGITFEFNNLLENIENYRDIFENENIELYGFDLKEK
                     LKVIHRKEIDVKNKLFDISLAEYLTGKTIKEQDIFKKVEKYKKFIKQMKMEKLYYEIE
                     IPLINVLIWIEKNGIKVDKEYFESLSKQLEEKILNLENQIYTQAGEVFNINSPQQLSK
                     ILFEKLKLPAKKKLKTTYSTDTIVLNELAALHPLPNLLIQYRELLKLKSTYVDGLLSF
                     VKNSRIYPTFNQLSTATGRLSCSNPNLQNIPVRTEIGSKIRKGFISEDNSFLLSFDYN
                     QIELRILAHFSKDNYLIEAFNQGKDIHQETGKFLFPENYLFSPNINNLSKEQIRRIAK
                     TINFGIIYGISPYGLSHQLNISVQEAKFLIDSYFEKFRGVKNYIEKTIKDIEENGYVE
                     TFFGRRRYIPEIKSPNKNLQEFAKRAAINMPIQGTSADIIKLAMVKIYNKFKKNNLQS
                     KIVLQIHDELILEVKENELEIIYRIVKDIMENCVTLQVPIKVDVKKGKNYLEMETINI
                     TLR"
     gene            74210..76240
                     /locus_tag="PLW95_00390"
     CDS             74210..76240
                     /locus_tag="PLW95_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21126.1"
                     /translation="MKKVRYHLRFQVIPDNKVEENAEKLADFCLKHKIEEVVLFLAAE
                     EWNNGLLSKKEEDLWFDTISKAKKTLEDKGITVSLNPWMTVLHLDRGRTFPYDRKFKP
                     MVSPFGEQSKACASFADKNWQKYIQNLYGRFAKLYFRVIWIEDDFRYHNHSPLTWGGG
                     FEKDICNRFSKKIGRKAKRKEILEKILQQGPPHPWRSKWLEVWREIQLEVASGIKEAV
                     RKNTKGKTKIGLMSSHPSVHSIEGRRWKSLFEALSIEKNIAHRPHFAFYSEVPGRDKI
                     YSIMMLDIQRKFRPSFCEVAPEIENFPFTVWSKSDTSTWSDMAFSLFFGSDALLLDLF
                     PMTGNSIIDEDKGIGKLLDRSYDSLLWISNRFSKENNLLGVGVPWKEDASEKVHTTKG
                     LSMEELIVDPLPAWRTLLSCGIPASVEKQNVNAIFGNSAWIFDDKEILEMLESGLLLD
                     GNSAKILCERGFSKYIGLEYKKTVGREESKYSLEVIINENSGIKKGIYLSVNLTEKFN
                     IFEPDRQTIVWSEVITPEKEKVGVGTTLYMNEKGGKVAVIVPDILNFNFFRQTIMQNL
                     IKYLYNMKIPFPIVSGSPYLFPMYFKGEKKDSLVIFNGGTDSAIVQFEIDKKKNTFGD
                     ITILKPLEKPTAGNLLKRGNTFWKLSSSIPYMSFFIMEFFKNKNYNNTNKEE"
     gene            76244..76741
                     /locus_tag="PLW95_00395"
     CDS             76244..76741
                     /locus_tag="PLW95_00395"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOV21127.1"
                     /translation="MKVLKDKNVLGIFVCGIIVVLVLVFCILMTFKSRVNIASVDLSK
                     IINEHPAMEEAMKNFQKELDNQQKKYDKLKDEEKAKEQQKIQQEMTQLAIQMQQNVVK
                     TAIGDVERIAKKNGYNYVIDKNALIVGGKDITDEVLKELKQNKQEKVQNKENTSDMPV
                     IPVNP"
     gene            76874..>77000
                     /locus_tag="PLW95_00400"
     CDS             76874..>77000
                     /locus_tag="PLW95_00400"
                     /inference="COORDINATES: protein motif:HMM:NF022596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 family protein"
                     /protein_id="HOV21128.1"
                     /translation="MLITFGVLLVISGLFFYKGIKFPLGKLPGDILIKKENFVFYF"
BASE COUNT        30480 a         9424 c        13106 g        23890 t
ORIGIN      
        1 ggtattgtta atgggaaaga cccttctgct tttaaaacat ttattcttgt tgattaaaaa
       61 tttaaaagag ctgtaataat ttagtttttt gaccgaaaca tacaaaagaa atgtggtaaa
      121 ttctcagtaa atcccagaga aattttatta agagaggagg tgatagaaat gaatagaaga
      181 aaagagagta tttcagtgtc cgatgagtat tgtggcggag ggtttaccct gatagagttg
      241 cttgtggtaa tcgcgataat agcgatttta gcagctatgt tattacctgc tttatcacag
      301 gcaagggaga aagcaagaca gtctgtatgt atgaataact taaaacagtt gggattagca
      361 atccagatgt atgttcaaga taatgatggc tggcttccta atatgtatga tgggacttac
      421 tggccaacaa aactttctat gtatatagga ggggcaactt ttacttttgt tccttggaat
      481 ccaggatggt cagcaggaac accaaagaga gtaaaggatg tatttatttg tccaagtgga
      541 ataaaagaat tacattgggg aataaattat gcatataata aaacaatagg ggattatact
      601 ccaagatggg ggtatcctac tgttagttat tgtgatcctg agaaacttga tagaataaag
      661 aatcctacac gtgtattttt agtaattgat caacggacta aatcttgttc agaaattgga
      721 aacgattgga atgctgggta tgttgattac aggcataatg atggagcaaa tgtattatgg
      781 gcagattcac atgtaaattg ggcttctcgt tcagaagttg caaggtggag agcaacagag
      841 tgggtaccat aaaaaaggag gaaaaaagaa tgagaagagt tggagtttat gtatttttag
      901 tatgtttttt attttcattt atagcaaaag cagacaattt attaaagaac ccggccaata
      961 tatacgagga agattattaa aatgatagaa aaaaatcttt atttatccac tgctattccg
     1021 attactgata tatcatctcg aatgttcttc tttgaagaaa accagccatc aatttctttt
     1081 ggaattcata aagtttatgc acctattacc aaggtaccta ttataatgaa aggactaact
     1141 aaaaatacca tatcagtttc ttcctttgcc agtactatca taaaacttgg tccatcccat
     1201 tatcgactct attcaacaaa ttatccgttt agtttcaaaa caatgtatat tgcgttatgg
     1261 gaatctggag atgggataat ctggtatcct ctaaaagtta gtagacacgg caaattttca
     1321 tctaacagta tatgcattaa tggaatcccg gggaatcaag atatgatagg tcaaccccag
     1381 gttcttcatt tagctgataa tgtgtggtat atgtatttct ggaaacataa agaaggacat
     1441 tgtcgttact taagagcgga gagtcgggat ggattaaact gggccgttgt ggattttgat
     1501 aatcctgtgt tgtatcaccc caacgaccaa ggattgtatg catgggcaat gggaatttct
     1561 ttacgtaaga ttaaaaacct acagataaca aggaaagaag ctgaaaaaca tttgaagtta
     1621 atttccaatg atgctactta tgtttattac aacgaatatc taaaacggtt tgaatgttat
     1681 tctgtctggc tccatcctga agttagggaa agatcaagat acaaaggaga agcaggaaaa
     1741 ccaaaaggta ttatacgctt tattcacaaa cgtgtcagta gcgatgggat aaactggagt
     1801 tatcctgaag taattctttt gcccgattac cgggacccat gggatttaca attttattat
     1861 ttatctgtac agtggcatga taagtggttg attggtaact taggatatta caggaccgaa
     1921 gaggggcagg atacaactga tatggattta tgtttctcaa aagatgggca atcttggcat
     1981 cggccagtaa gaggtggatt tattcctcgt gttgaaaata aaccagatgc tatgggtatt
     2041 tatgctccta atacttggat agacaaagga aacaaatggt tgtgccttta tactggaaca
     2101 ccggaccctc atgatgaaga tatacatcgt gcttcaatta tgggtgcaac atttgataaa
     2161 aaccgctttg tagggttgca agcaggcaaa gttactggcg gttttttaag tgatgttttt
     2221 atcctccaga atgccaatat aaaagtagat gcttgtatca aaggatggat gcgtgcagag
     2281 atatgtgatg cttttggaaa tgcaattaaa gatttccatc ttatggattc ttatgttatt
     2341 tctggtgata aatcagaaca tattctacgc tggaagcaat cagataccaa ggatctaaaa
     2401 tatagagcgt tgagattgag aatagaattt agagaaggag ttatttatag tttttgtttt
     2461 taataaaaaa ggaatattgt tgtaaaccct gtagagtaaa agaatgacat atatagagag
     2521 agagagagta ccttttataa gttttaatgt acgtatctac taattatggt tctcttttaa
     2581 aaaagttgta gtaatatttg tccaaaggat tcccatggcg tattttataa tccgtagtca
     2641 aaagtgagta ggcaacgggc gaggatttgg attagatata ataagaaaat ggaaatgaaa
     2701 gtacataaaa gaattttaat accaattaaa gaagaaaaac ataagtacaa agaggataca
     2761 tctttcggga catcaattat gatggtaaat acaacatata caaaaaaaga tggtttacat
     2821 ttacttcaac tatattctga agaagtagca tctgatacat cgggtaaatg cttccaaaga
     2881 acaagtaaag acaatgggaa aacatggtca aatcccatat tagtttttga accacaagaa
     2941 acagaagaag gagttataag atggggagaa agttgtttat ttttagatga gggaaaagaa
     3001 tgtattttcc atttttataa tttacatctt taccctaaaa atattttttc aagagaagtt
     3061 caagaataca ccaggatatt ctatagaata agttttgatg aaggaaaaac atttagtaat
     3121 ggtaagcaaa taattcaaaa gggatttaac gaaaccaatt gggcagaaga tgtaatatat
     3181 ggtaaaaata gcattgcaat atcattttgt gctcctttaa aacttaacag tgggaaaata
     3241 cttttgccat gtcaaaaagt tcctttagag cataaaggag acaatccatt tcttatttca
     3301 tatgtagcag ggtgttttat tggtgaatgg aagggacaag agatagaatg ggaattaagt
     3361 caaatggtaa aaatagaccc acaattatct tcaagaggtc tatgtgaacc aacaatcgcc
     3421 caactttata atggagaact cttaatgata atgagaggaa gtaattatac aattccttct
     3481 gtacctggat ataaatggtt ttcaatatca aaagatagtg gttatacctg gtcaagtcca
     3541 acaccattaa aatatgatac aggagaaaat ttcttttcac ctgctactgg ttcaagatta
     3601 attcgtagta caaaaaataa gaaactatac tggattggaa atattgtaaa agaaaatcct
     3661 gatggtaata gaccaagata tccattacag atagcagaag ttgatgaaga aaagaaagca
     3721 ataaaaaaac atacagtaaa tataattgaa gataaaagag aaagtgattc accttttgtt
     3781 caattctcaa atttcagagt atatgaagat agagaaacag gagagtttgt cttaataatg
     3841 gcaagaatac aggaaagagg agaaaaagac cttagttctc ctgcttatga atacagaata
     3901 gaagttttat aaatataaaa tgggaaaaat gaaatttctt gttattggtt taggttcaat
     3961 gggaaggcga agaataagat gtcttcagta tttaggagaa aaagatatta ttggatttga
     4021 tatcaggaag gatagatatg aagaaacaga aaaaaaatat ggaattaaga cattttctga
     4081 ctttaatgag gcatgtgggc aaaatccaaa tgtatttata atatctgttc caagtaattt
     4141 acatcatttt tatgcaatgg aagcagtgaa aagaaataaa cactttttca ctgaaagtaa
     4201 tttccttccc gaaggaattg atgatttagt aaaagtagaa aaagaaggaa aaattatttg
     4261 tgttccaagt tttactatgc cccaccatcc gtcagttaaa ataatgaggt ctggtattga
     4321 ggaaggtaag atagggaaag tatattcttt tacctatcac ctatcttctt acctgcctga
     4381 gtggcatccg tgggaggact acagggatgt ttattatggg aagaaggaga cacctggctc
     4441 aaaagaaatg gttgcctttg aacttacatg gattgtctgg ctacttggaa gagttcagga
     4501 agtatgtgcg tttaaatcaa aaatgtcctc tcttgagatt gaatttgagg atacatatca
     4561 aataatattg aaactaaaaa atggggtatt gggacacctt cttatagatg ttgcatcact
     4621 gccatctgga aggaatatga agattttagg agagaaagga acattttgct ggaattctga
     4681 agaagagatt atcaagtggt ttaacactga aaagagaaaa tgggaagaga taaagggaga
     4741 caaaggtatt gaggaggcag gttattcagt ccatactcat gaggagatgt acatagaaga
     4801 gatggagaac tttgtaaaga cggtgagggg agaaaagaag tatccttata catttactca
     4861 agagaaacat ataatagata tccttgtagc aatagagcaa agtatttctc aaaaaatagt
     4921 aatggaggtg gaagatgagg tgtagaaaag gacaaaaatt gtggcaaaaa gcaaaaaaaa
     4981 ttataccagg tggaactcaa cttctttcaa aaacatcaga acagttttta ccagaatatt
     5041 ggccttcata ctacaaaaaa gcaaaaggaa taagtgtctg ggaccttgat gggaataaat
     5101 ttctagattt ttctataatg ggagtgggta gttgtattct tggatatgct gaccctgatg
     5161 ttaataaaga tgtaaaaaaa gttataaata atggaaatat gtgcacactt aattcacctg
     5221 aagaagttga attagcggaa ttgcttttaa aaatacatcc ttgggcagga aaagtaagat
     5281 atgcaagaac aggtggagaa gcaatggcaa tagcagtcag gataggacgt gctttttctg
     5341 gtaaagataa gattgctttt tgtggatatc atggatggca tgactggtac ttatcagcaa
     5401 atcttgcagg agataaaaat cttgatggtc atcttttgcc tggcttaaaa ccacttggcg
     5461 ttcctcgtgg tctttatgga actgcctttc cttttactta taatcatatt gaagaacttg
     5521 aggtaataat caaaaacaac gagataggtg taattgtaat ggaaccaata agaagttatt
     5581 atccagaaaa tggtttttta gaaaaagtga gaactcttgc taataagaat aaggcagtct
     5641 tgatatttga cgagataacc agtggcttcc gtatgaaggt aggaggcgca tatacacttt
     5701 tcaatttaga gccagatatt gttgtatatg caaaagcaat gagtaatggt tatcctatgg
     5761 ctgctgtcgt aggaagagaa aacattatgg ataaagcaga agagagtttt atcagcagta
     5821 cttattggac tgaaaaaata ggaccaaatg ctgcattggc aactattaaa aaaatgaaaa
     5881 gagaaaatgt cccttcacac ctgataaaaa ttggagaaca gataacaaat gggtggaaag
     5941 aaatagcaaa tgaatgtgga ctgaaaataa aaatatctgg tattccccct cttacaaaat
     6001 tttctttttg ttatgagaat tcacaggaat tgataactct ttttactcag gaaatgcttg
     6061 aaaggggatt tcttgcaaaa gatagtgttt atgtttcata tagtcataaa gaaacagatg
     6121 tcgaaatata tatgtcagcc gtaaaagaag tttttaaaaa aattaaaaaa ggtatagata
     6181 caaacaaaat tagtaaaatg cttaaaggcc cagttgctca tacaggattt aaacgactta
     6241 cttaattttt aggaagaatt taaaaatgat agaacttgcg tttttaggga caaatgaggg
     6301 aaatgggcat atattttcat ggagtgcaat tataaatggg aaatatgatg aacaactaat
     6361 gaaagagtgt ggttatccag ttatatatga atatctatca aaaaacaaaa aagatattgg
     6421 aattccggga gcaaaggtaa gttatgtttg gacagaagat aaaaaatatt ctgaaaaagt
     6481 tgcaaaaaca agttttatag aaaatgttat tgatacccca gagcaggtaa tagggaaagt
     6541 tgatggagtt gttataacta cagatattgg aagcaaccat ttaaaattag caaaaccatt
     6601 tattgaagaa aacatacctg tttttataga taaaccatta acagataatg aaaaggactt
     6661 aaaaaaattt atagaatatt ttaaagaagg caaaccaatt ctttcatcat caagttacag
     6721 gtattccaag aatatagatg atttcttaaa ggaaaatact gataagatag aatttgtaag
     6781 gtgtataatg aacaagacat gggagagata tggagtacat gcaatggaag ggttatataa
     6841 aattatgggt tcagggatta agagtgttat aaatttagga gataaaaaca taaatgtagt
     6901 ttatatagag tatagtgatg aaagaaaagc agtcattgaa aatatctata attctaaaat
     6961 tactgactat gatattataa caccaaagaa tacttataca attgatgata caaacacatt
     7021 ttatatgttt aaaaagcaaa tggagatatt tatcagattt attgatgaaa gaaaatatcc
     7081 gtatccatat gaagaaacga ttgaaataac aaaggttatt atagcaggaa taatatcccg
     7141 tgaggagaaa agaaaagttg aactgaaaga gatactatat taaaaaggag aatattagaa
     7201 aatgggaata ttagaaaagt tttcattgaa aggtaaggta tccatagtaa caggtggagc
     7261 aggtctttat gggaaaggta ttgttgaagg actggcagaa gcaggaggat gtgtttatgt
     7321 tgcatcaaga aatgttgaaa actgcaataa gttagcagat acattaagaa aaaaaggata
     7381 cagtgttgca ggggcaaaat tggaccagac agatacggaa agtattaaac aactaagaaa
     7441 tagagttatt gatgaacagg gtaaaataga tattctaata aataatgctg ttttgagacc
     7501 tatggagtca cctgatgcac caatagagac ctttgaactt tcaatgaagg tcaatgccac
     7561 aggtgttgta aatatcacac gacatattgc agaggagatg aaaaagcaga ggtcaggggt
     7621 aataataaat attgcttcta tatacggtgt gcttggtatt gacccttatc tttatgaagg
     7681 cactgatatg ggattaagtg gacctgacta tttctttcat agagcaggaa ttataaatct
     7741 tacaagatat tatgcttctc ttcttggtcc atataatata agagttaact gtatctctcc
     7801 aggtgggctt tttaataacc agcatcctgt gtttgttgag agatataaca gaaaaacatt
     7861 tttaggaaga atggcaaata gagaagacat aaaaggagta gtggtatttc ttgcatctga
     7921 tgcaagttcc tatattacag gagcaaatat tatgatggat ggagggcttt ctgcaaaatg
     7981 aagaaaaaaa tacttataat aggtgcaggg ggaataggga aaaaacatat agatggattt
     8041 ttgagaactg gtaaatttga aatttctgct tgtgatgttg atagtaagaa gttaaaagaa
     8101 gtagaagaaa aatttaaaat taaaaattta attattgatt ttaattcagt taatttaaga
     8161 aattttgatt gtgttttaat ttcagcacct gcaaattttc atattccaat tgctattaaa
     8221 tgtgcagaaa acaatattcc atttcttgtt gaaaaaccaa tttccgttga tttaaaaagt
     8281 atagataaac ttttaaaaat tgttgaaaaa aataaagtaa aatgtggagt tggttttaca
     8341 agaagaagcg tcccatcttt taaaaaactt aaagagataa ttgaaaataa aaaatgtgga
     8401 gaaacaaaaa tggctgcttt tcatgttgcc caagattaca gaaaatatag gccggactat
     8461 gcagaaatat attttgcaaa agaaaaaatg ggtggagggt gtattcttga tgctgtttct
     8521 cattctatag accttgcaca atggtatata ggaaaaccag aatatggata tgcaatttat
     8581 gataatcttg actttggaga aaaaattgaa acagaggata gctctgcaat aataagcaaa
     8641 tttgataatg taatagttaa tttttattgt aatttatttc aaaaaccata tgaacttttt
     8701 attgaatttg ctggaacaaa aggaaatgtg agatatattt cagaaaataa acaaatttca
     8761 aagatacttt tttcagataa tgatgaagga agatgggaag aactttttac atttgaaaat
     8821 gaaataagtg attattattt ttatcaagca gagaactttt tacaactaat aaatggtgaa
     8881 aaaacatcat ttacaaaatt agaagaggca gttgaaaatt taaaattctg tcttaaactc
     8941 aaaaaaggag gcaaaaatga aattaaaaga agttaaatgt atatggtggg atgggtatca
     9001 taatgctttt acagacatta ttaaatacaa agggagttat tttgtttgtt ttagacatgc
     9061 aactgaacat ggattgaaag gacaaggaga aatatatgtt ttaaagtcaa ataaccttga
     9121 aaattgggaa cttataacaa aatttccgcc tttagaagat agtagagacc ctaaattttt
     9181 tatttttgaa gaaaaaattg gagttttatt ttttgcttat cctgatataa acacaagaca
     9241 gataagagat gggtatattt cttattctga tgatggagaa aatttttcac ctgttataaa
     9301 aatttcaaag gacaatttat gtttttggag aataagaaat tataaagaaa aactttatgc
     9361 aactgcctat aaaggtgggg aaaaagaaga agattgggga agttatttat ttgtttctga
     9421 cgatggtgaa aattttaata aggtatcaac aattttagaa ggcgagtacg caaatgaaac
     9481 tgacttatta tttgaaggag atatttgtta tgcaattgtc agaagagaaa atttacaaac
     9541 gcctgcttta tgtatctcaa aatatccttt tgataaatgg gagtcctata atttaaacaa
     9601 aattattcag ggaccctgta tatttaagtt taaaggggaa atttatattg ctggaagagt
     9661 taatacatta acaaaaacag gtattttaaa acttgacctt gaaagaaaaa gagtaaaaga
     9721 tgaaattatt cttccaagcg aaggggatac ttcttattgt ggtgtggttg ttgaaaataa
     9781 taaattttat ttatcttatt attcccaaca tgaaattgaa aaaagagaaa gcaaagttgg
     9841 agaaaatgca gcaggtatat atattgctga gataggagaa taaaaatgga aatgaaattt
     9901 tttggggtgt atcctgcatt attaacgcca tttgatgaaa aagaaaatgt tgatgaaaaa
     9961 cgactaagaa aaattataaa atttctaatt tcaaaaggag tagatggact ttatatatgt
    10021 ggaggaacag gggaagggat gttgatgaat gttgatgaaa gaaaaaaagt tgcagaaatt
    10081 gttaaagaag aagttggtaa taaagtaaaa ataataactc atataggagg tgctttaaat
    10141 acaaaaaata caattgaact tgcaaaacat tctgaacaat taaaaattga tgctttatcc
    10201 tcaattccgc ctttttatta taaattcagt tttaaagaaa tttacaagta ttataaagaa
    10261 gtggcagaaa acacttcttt accatttttt atttattata ttcctgtaac aacaggtgtt
    10321 tctattacga tagatgaaat ttgggaactt tcaaaaataa aaaatataat tgggattaaa
    10381 tataccaatt atgacttttt tactttacag aacataattt taaaaacgag atggatatct
    10441 ttttcaggtc ctgatgagat gtttttacct gctttaacta tgggagtagt gggttgtatt
    10501 ggttcaactc aaaatgtttt gccagagata tttattgata tttataaaag ttttaaagag
    10561 ggaaatatta aaaaggcaat ggaaggacaa aaaagaataa caattgctgt atctatttta
    10621 aaaaaatatg atggaataac tgcatggaaa gcaacaatga agtttagaga aattgatgtt
    10681 ggatatgcaa aaaaaccatt tgaaaatttt ttagaaaaga gtaaagaaaa ggaattaaaa
    10741 aatgagtgga aaaagtattt tcctgaattt tcagaaggta ttaagtagtt ttttattttt
    10801 aactattgtt gcaattttta tatcccattc tatggaggaa ataaaaatga aaaaaattga
    10861 tttgcttcct gcatcagaaa aaaatccaag aaattctgaa ggaagtatga ttgaattaaa
    10921 aaatggaaaa ataatgttta tttattcaca tttttatggt ggtcaaggag ataatgcact
    10981 tgcttttctt gctgccagat attcctttga taaaggagaa agttggacag aaaaagatga
    11041 agtaattatt gaaaatgaag ggaaagaaaa tgttatgagt gtttctctat taagattaaa
    11101 aaatggagaa attttattag gatatttaat aaagaactct cttaatgatt gtaaatacta
    11161 tataagaaaa tcatctgatg agggtaaatc atggagtaaa aaagttcttg taacaaataa
    11221 ggagttttca aatggaaatt attttgttgt aaataatgat agagtaattc aattatcaaa
    11281 tggcagaatt attgttccat cttctcatca tccttgttca actggcaaat gggacgattt
    11341 tggacccggg gaagtaattg ttttttattc agatgataat ggaaatacat ggaaatcaag
    11401 tagaaatatt ctatcatctc cagaaaaaaa tgataaacgt ggattggaag aaccaggagt
    11461 agttgaactt agagatggaa aaattttaat gtggataaga acaaatttag gtcgtcaata
    11521 ttattcatat tcagaagata gtgggcaaac atggagtgaa gtatctccat ctcctttagt
    11581 ttctcctctt tcaccagcat ctatcaaaag aataccaaaa acaggagacc ttctttgtat
    11641 atataacgac cattctggga aattccctta tccagaattt caaagcagga caccattagt
    11701 ctcagcaatt tcaaaggatg agggtaaaag ttggcaaaac catctcttaa tagaagatga
    11761 cccaaaaggg agattttgtt atacttcaat tctgtttatt gacaataaaa taattttgag
    11821 ttattccgca gaaaatgttg aacaggcatg gggactatta agagtaacta ttttaaagat
    11881 ggaggaagtt tataatggag aaaaaaatac ataaggattt tcatggggct ttaagtgttg
    11941 gttttgaatt tctatctgat aaatatggtg aaaaggttct tgaagaatat cttattgatg
    12001 ctgcagaaac tatttatagt gatttaataa aaaaaattaa aaaaaatgga atagttgaat
    12061 tagaaaatta ttggagaaaa atttttacag aagaagaagg taagtttttt cttaaaagag
    12121 atgacaataa aaaaattgaa ttagttgttg aagaatgtcc tgcaatttct catatgaaaa
    12181 aagttggtta taaaatttac aaaaatttct gtattcagtg tgaagttata aatagagtaa
    12241 tatcacaaaa aacaggtttt atttctgaaa taacttatga tgtaaaaaat gggaaatgca
    12301 agcagatatt caaaaaagag gagagaacat gatatcatca ggagaattta tttttgctta
    12361 taatgaactt tttaaattca tacataaaaa atatgggaaa aaagatgtta tatctttatg
    12421 ggaatttatt tcggataaat ttcttacaaa tttagatgaa cttgttagag aaaaaggaat
    12481 atctggaatg gctgaatact ggacacatac tttaacagag gaaggtgcag aatatcaaat
    12541 gtctgttgga gaaaattatt ttgagatatt tatgaaaaaa tgtccttctg ttgggaaatt
    12601 aaacaagaca aaagtaaaaa aatatcctta ttattgtgaa cactgcaaaa ccctatataa
    12661 aaaagtaata gaaaaatacg gatttttata taaagtaaaa attattgaca aggaaaaagg
    12721 gatatgtaaa gttttcgtca aaagaactaa aaaaaggaat tttcccattc aataagatat
    12781 tttttaaatt cctttccaag tacataattt ccaatttctc catttttttt aattactcta
    12841 tgacagggaa ttgaaataag ataaggattt tcttttaaaa ttgaagatat ataccgaaca
    12901 ttatttttca ttccgagttt taaagcaata taagaatatg tttttgtatg tccaaatttt
    12961 atttttttta attccaccaa aagtttctct tttaattttt ttttgttcat gttaaaatta
    13021 tttttgtttt gatttttttt attatataat tgtaattcaa aaatggaaaa atatatacaa
    13081 tcttttatag aaatggtaaa atttaatgaa aatggtttag ttgttgtagt tgttcaggac
    13141 ctaaatggaa ttattttaat ggttgcatat atgaacacag aagcaattga aaaaacacta
    13201 caaacaggta aaatgcacta ttacagcaga agtagaaaaa aattgtggct taaaggtgaa
    13261 agttcaggaa attttcaatt tgtgaaagat atttatattg actgtgataa tgatgctttg
    13321 cttttcagag ttcagcaaaa atgtggagca tgccatagtg gatattattc ttgtttttac
    13381 agaaaatttg aagatggaaa tttcaaaatc atagaagaaa aaatttttga accagaggat
    13441 gtatataaca aggaaataaa aaaatgctaa aaagttatta ttacaaacta tttttgataa
    13501 ttgcaataat tggtatattt atatatctga tatatccagt tgataaaaaa attaaaaaag
    13561 gccttgactt gcaaggagga attcatattg ttcttgaagt agaaaaacca ccagaagaaa
    13621 cagaaaatat aaatgaatta actgatagag cacttgaaat aataagaaac agaatagatg
    13681 ctcttggggt ctctgaacca ataatacaaa aagaaggagc aaacagaata atagttgata
    13741 taccaggggt taaagaacca gaaaaagcaa tagatataat tggaaagacc gccttattgg
    13801 agtttaaact tgttaatgat gacccaaaag cacttcaatc tgcattagaa ggcaatcctc
    13861 cagaaggata tgaggtttta tatacaactg aaaaagatga aagagggata gcaagaaagg
    13921 gttctcctat tcttgtaaaa aaagaaccag aacttacagg taaatatata aaagacgctt
    13981 atgtaagtta tgacccgggt ggtttgccct ctgtgagtat tgtttttaat agtgaaggag
    14041 caaaaatttt tgaaaatgta acaacaaata atgtaggtag acaattggca attgttttag
    14101 atggtgtggt aaaaagtgct cctatgataa gagaaagaat accagaaggg aaagggcaaa
    14161 ttagtggtaa atttaatatg gaagaagcaa aagagttagc aattgtttta agagcaggtg
    14221 cattacctgc aaaagttaat atgatttatc agcaaattat tggtccaact cttggaaaaa
    14281 aatatattca acaaggtttt cgtgtaattc tttatggcgg aatacttgtt tttattttta
    14341 tgattttcta ttattctgta tttggtcttt tggctgattt tgcccttgct ttaaatatac
    14401 ttattctttt aggtataatg gcagggatta aaggtgttct tacaatgcca ggaattgcag
    14461 gaattgcttt gactattggt atgtcagttg atgcaaatat tttaattttt gaaagaataa
    14521 gagaagaaat aaaagccgga aaatcaccaa aaacagcggt tgatgctgga tatttaagag
    14581 catggagtgc tatccttgat tctaatataa caacattaat tataggagca atactttttt
    14641 tctttggaga aggttctata aaaggttttg gattaacttt aagtatagga atacttgcaa
    14701 acttatttac cgcggtttat gtaacaaaag taattgttga ttactttctt ataataaaaa
    14761 aggttgaaaa aatacggata taaaaatgga actgataaaa aaaacaaatt ttgattttat
    14821 ggggaaaaga aaaattgcat atttattttc tattattgtt attttatggg gaatgacaac
    14881 attttttata agaggcaaaa aaaattttgg agttgatttt acaggtgggg atttattaca
    14941 aattgccttc aagaacagtg tctctgttag tgaaataaga gatactctat caacattaaa
    15001 tattgggtct tttacagtac aggcagttgg aacagaagaa aaagaggtaa taataaaaag
    15061 caatccaaat acatctgata caatactaaa agcaattcag caaaaatatg ggcaggatat
    15121 tgaaataaaa aataaggcaa ttatttcgcc atcaatgagt acaagtttaa gaaacaaagc
    15181 acttttttca tttttgcttg gaataatagg tatacttatt tatttagcaa taagatttga
    15241 attcagattt gcagttggag caacagttgc aatttttcat gatttgcttt ttgttatctc
    15301 aatacttgca attgcaggga aacaaattga ttcaactata attgctgccc ttcttacaat
    15361 agcaggatat tctgtaaatg ataccgtggt aatttttgat agaataaggg aaaatgtaag
    15421 gaaaacaaga agtgataatt atataggtat ttttaaccta agtataaatg aaacattaag
    15481 tagaactatt cttacaggac taacaacaat tgtagttgtt atattattct tcttctttgg
    15541 tggggaatca cttcatgatt ttgctttttc tttacttggt ggatttatta ttggaacttt
    15601 ttcttctgtt tttgttgcgt cggcattggt tattgactgg catagaatta gaccttataa
    15661 aattaaatta tgaaagtaat aggtggaata cataaaggta aaaaactctt atttataaaa
    15721 gaaaaagata taagaccaac aaaagatgtt gtaagagaag caatttttga tgtcctgcgc
    15781 ggatggatag ttggaaaaaa aataattgat ctgtttgctg gtacaggggc acttgggatt
    15841 gaggcaataa gcgaaggtgg agaaaaggtt atattcgttg aaagtaaagg gaaaatagtt
    15901 gatattttaa aaaaaaatgt aattaatgtt ggaatagaaa aacaggtatc tattttaaag
    15961 ggtggagtag aagagaaaat caaggagatg agaaatttca aatatgacct tgtaattgca
    16021 gacccacctt atgattttcc cgtaaacaaa ttgaaagaaa tacttgaaac aattatttct
    16081 tccgaagtaa taaatgaaaa tggaattatt attgttgagc atagaactaa atattcaata
    16141 ccagaaagtg ctaaatttat tgtctacaaa aataaaaaat atggtaaaac atctgtttca
    16201 tatttaaaaa ggaaagaaaa tgaagaatat agcagtctat ccgggtagtt ttgaccctat
    16261 aacaaatgga catattgatg ttattcatag gtgtctgaaa attgtggaca aaataataat
    16321 tgctatttca tcttttcaaa gaaaggactt tcttttaaca attgaagaga gaactgaaat
    16381 aattaaagaa acatttaaaa atgatgatag aattgaaata gataaatttg atgggcttct
    16441 tgttaattat ctaaaagaaa aaaatataaa tattattgta agaggactta gagcaatttc
    16501 tgattttgaa tatgaattcc aaatggcttt aacaaataga aagttaaata aaaatattga
    16561 aacaattttt ataatgccag gagaagaata tttttttata agttcaacaa tggtcaagga
    16621 aatagcaaaa ttaaaaggcc aaatttcttg ttttgtccct cctgctgtgg aaaaaaaatt
    16681 aaaagaaaaa tttaactatt aaatgtaaaa tttaaaatag aaaaaataca agacaaaaaa
    16741 tgaaaaagtt ttaaatctat taaagaagga gaaaaaaatg ccaaatccta aaagaaaaca
    16801 ttcaaaaagt agaacaggaa agagaagaaa ttcaaaaaaa attaaaaatg tctccctttc
    16861 aaaatgccca aattgtggtt ctttaattac gcctcattct gtttgtcctc aatgtggata
    16921 ttataaagga gaacttgtta tccagaagaa agaaaaaaga aaaaaacaat gaaaaatatc
    16981 tctattgcac ttgatgttat ggggggggat aaagttccct tttcaacagt aaaaagtcta
    17041 gaatgtatta aaaaaggtga taatttgaaa atttttcttg taggtgaaaa agaaaaattt
    17101 aatcatctct tatcttcttt aaatcctgat ttgtactcaa ttgttgaatg taaaaagttt
    17161 attccgatgg ataaaaaggt ggggctgaaa cttctgaaag agacagatac aacaatgtca
    17221 gttacattac gacttttgaa agaaaagaaa gttgatgtag cattaagtgg tggcaataca
    17281 gcagcttttg ttgctcttgc aatatcacaa attggtttaa ttaaaggagt agaaagaccg
    17341 gcaattgcag ttttatttcc taatgcttca aagggaaata caatttttct tgatgcagga
    17401 agtaatataa atgcaaaacc aatccatttt ttaagttcag caattatggg agcaacctat
    17461 gcagaaattg tctttggtat taaaaaacca aaagtatctt tattaaatat tggtgaagag
    17521 gaaacaaaag gtgatgattt aagaaaagag gcattccaat tgttaaaaaa tgcaaaagaa
    17581 attaatttta ttggaaatat tgaaggacag gacattttca caggaaaaag cgatgtaatt
    17641 gtaacagatg gatttactgg aaatgttgtt ttaaaaacat cagaaggtat cagcagatct
    17701 ttcaaaacac ttcttttgag atatttaaga gaaaatattt ctggtaaaat tgcaactttt
    17761 cttattaaga aaaatttaag ccaatttgca aaaatggttg attatgctga atatggagga
    17821 gggatattac ttggagttga tggaatagtc cttatttctc atggaaggtc ttctccaagg
    17881 gcaattttta atgcaatgaa attaggtgaa aaaattgtaa aatcaaattt tttagacatt
    17941 atgaaagaaa aagtgaaaga tatggagagt taaatgggag taattataac tggaactggt
    18001 tcttatttgc ctgagattgt tttaacaaat agagatatgg aaatgatggt tgatacctct
    18061 gatgagtgga taacaacaag aactggaata aaagaaagaa gaattgctcc aaaaaatatt
    18121 tcttcttctg atttagggaa agaagcaatt ttaaatgcat gtaatgatgc aaatattgta
    18181 ccagaaaaaa ttgactttat aatttgtgca acaattactc cagataaatt tttcccttca
    18241 acttcttgtc ttatagaaca caaattaggg attaagaaaa aatttgcatt tgatatttct
    18301 gctgcatgtt ctggttttct ttatggactt gaaattgcaa gaaatcttat agaaacaagt
    18361 ggctatcaaa caggagcagt tgtagcagca gagtgtatga gtagaataac tgattataca
    18421 aatagagaaa catgtgttct tcttggagac ggagcaggag cagtaattgt tcaaaggtca
    18481 gaagaaaatg gagtaatcgg gtcatattta tcttctgatg ggggttatga atatttgctt
    18541 catgcccctg ctggtggttc aaaattgcct tcttcttttg atacaattga aaaccgcctc
    18601 aactttatga aaatggaagg acaaccactt tttaaaattg caataattac aatgtgtgaa
    18661 agtataaata atatcttgaa aaaatataaa ttgaaaaaag aggatatatc gctcattatc
    18721 cctcatcaag caaatttaag aattatttta ggtgttgcaa aaaatatgaa aataccagtt
    18781 gaaaaatttt ttgtaaatat tgaaaaatat ggaaatatgt ccgctgcgtg tgttcctgtt
    18841 gctcttgacc atgcaaataa aaaacaacta ataaaaaaag gtgattatat cttaactgtt
    18901 tcttttggtg gtggattaac ttggggtgca aatctaataa aatggtataa aggggataaa
    18961 aatgaaaaat aaagttgtta taattacagg ggcatttgga ggaattggga aagcagttgc
    19021 ttataaattt tctaatttag gtgctaaact tgttctctgg gatgttatta ttgataatga
    19081 atttatcaac caatttacaa aaaaagaaac agaaataatt gcttcaaaaa ttgatataac
    19141 aaatcaaaaa gaaattgaag aagggaaaaa ttatataatt gaaaaatttg gaacaattga
    19201 tgttttaata aataatgcag gcataacaaa agacaaaatt attctgagaa tgacagaaga
    19261 ggaatgggat gaagtaataa atataaactt gaaaggtgct tttttatgta gtaaaattat
    19321 agggtatgaa atgtttttaa agaaaaaagg taaaataata aatgtcgctt caataatagg
    19381 acaggttggg aatataggac aggcaaatta tgctgcctca aaaggtggtt taattgctct
    19441 tacaaaagtt tgtgctaaag aatttgcaag aagtaatata aatgttaatg caattgctcc
    19501 tggatatatt atgacaaaaa tgacacagaa tttacctgaa aaagtaaaag aaaaaatgct
    19561 tgaaaatata cctctaagga gatttggaac tccagaggat gttgcagaac ttattgcttt
    19621 tttatcaggt gaaaattcaa attatataac aggtcaggtt attagaatag atggtggact
    19681 tgtaatgtag tactcggtta aatatcttga ccaaaaagtg gagagtaaca ggggttagca
    19741 gagcagagtt ggtgcttgat ttcctctctt atttcttccc tttgacaaag ggaagataga
    19801 aaggattttg gagatttcaa caagcacaac gggagtgaga ggggatttat aaatgaaaga
    19861 cataaaacaa tgtgctttct tttgtgcttt acaagtatat atcactgaac tctaatgtaa
    19921 aaaaaggagg aaaaaagatg gaaagagaag aaatagcaaa aatagtaaag gatattgttg
    19981 ttgagaagtt gggtgttgat gctaatgagg taacagaaaa cgcatctttt attgatgacc
    20041 ttggtgccga ttctcttgat acagttgaac tcgttatgga ttttgaggga aagtttggac
    20101 ttgaaatacc tgatgaagat gctgaaaaaa taaaaacagt tggagatgca ataaattata
    20161 ttgaaaataa tatttcaaaa aaataaaaat gaaaaaaatt gtcgttactg gacttggatg
    20221 tgttacacct gtcggaaata atttaaaaac aacatgggaa aatttaataa aaggtgtaag
    20281 tggtgttgat acaataaaaa gttttgatgt atccaatttt gatacaaaaa ttggtgcaga
    20341 agttaaggac tttcaattgg attttattca tccaaaagaa aaaagaaaaa tggataaatt
    20401 tgtccagttt gctataaaag caactgatga agcaataaaa gatagtgaaa tagaacttga
    20461 aaaagaaaaa aaagagaaaa taggtgtaat agtaggagca ggaattggtg gattaagagt
    20521 aattgaacaa caacacgaaa tacttttaag ttcaggtcca ggaagagttt ccccttttct
    20581 aattccaatg ttaattccag atatggcagc aggacaaatt gcaatttact ttggatttaa
    20641 gggagttaat tttgcaactg taagtgcctg tgcttcaggg gcgcatgcta ttggtactgc
    20701 tctttgtttt atgagagaaa ataattgtga tgtaatgatt gctggcggaa ctgagtcctg
    20761 tataacacca cttggacttg caggtttctg ctctatgaaa gcattatcaa caagaaatga
    20821 ccaaccacaa aaagcatcaa gaccgtttga taaagaaaga gatggttttg ttatgggaga
    20881 aggtgcaggc attgttgttc ttgaaacaga agaacatgca aaaaaaagag gagcaaaaat
    20941 ctattgcgaa cttgctggct atggaatgag ttgtgatgct tatcatatga catctcctga
    21001 cccaacaggt gaaggtgctt atcttgcaat gaaacatgct ctggtaaatt caaatattgt
    21061 tcctcaggaa gttgattata tcaatgctca tggaacttca acaaaattaa atgataaaag
    21121 tgaaacagat ggaattaaaa gggtctttgg agaaaatgcc tataaaatat atattagttc
    21181 aataaaatca atggttggac atttacttgg agcagcagga ggagttgaat ttgttataac
    21241 atgcctgaca atcaaagagg gaataatacc tcctacaata aattatgaat atccggaccc
    21301 tgaatgtgac cttaattatg tcccaaataa aacaattgaa aaagatgtga aagttgctct
    21361 ttcaaattct tttggttttg gtgggcacaa tattactctt gttgctaaaa aatatgaata
    21421 aaggagagaa aatggaatac tttcttactg atgaacaaca aatgataaaa gaacttgcaa
    21481 gaaaaatcgc agaagaaaaa attctaccaa aaagagcaga atatgatgaa aatgaaatat
    21541 ttccccatga tatagtaaaa gtacttgctg attcggacct ttttggtgtt tttgttccag
    21601 aaaactatgg cggacttgga tacggagttt ttgaattgtg tcttgttatt gaagaattaa
    21661 gtagggtttg tgggggaatt gctttatctt atgctggaac tgctcttgga acttttccaa
    21721 tagttctttt tgggaatgag gaacaaaaaa ataaatatct tccaccaatt gcaaaaggaa
    21781 aacttgctgc atttgcagta actgaatcta gctcagggag tgatattaca aatctaaaca
    21841 caacagcaaa aaaagacgga gattggtata ttttaaatgg aacaaaacag tggataacaa
    21901 atggtggtga agcagaaaca tacgtagtaa ttgcttcaac tgataaaagc aaaggtgcaa
    21961 gaggttcaac tgcttttata attgagaagg gatgggatgg ttttgagttt ggaaaaaaag
    22021 aaaaaaaatt gggaataaga gcatctgcaa caagagaact tgtctttacc aattgtaaag
    22081 tcccaaaaga aaatgtttta ggaagagaag ggatgggatt tttaattaca attaaaaatt
    22141 ttgatacaag tagacctggt gttgcctcac aggcattagg tatagcacag ggtgcgtatg
    22201 aatcagcact tgaatattct catcaaagaa aacaatttgg acaaccaata agttcatttc
    22261 aagcaattca acatatgctg gctgatatgg ctacacaaat tgaagcagca agaactctta
    22321 tttatcaaac agcaaaaaca attgactcag gagcaaaaag ctattcaaaa gaatcagcaa
    22381 tgtgcaaact ttttgcttct gatgttgcta tgaaagtaac aacagatgca attcagattt
    22441 ttggaggata tggctatatg aaagattatc ctgttgaaaa aatgtttaga gatgcaaaaa
    22501 taactcaaat atatgaagga actaaccaaa tacagagaaa tgttattggt attgaattga
    22561 ttaaagaact tgcaaaaaag aaaaaataag tgaaagagat tgtttttaac caggaaaaga
    22621 atacaagaat agataaatat cttaaagaaa aacttaatgt ttcaagagaa agaataaaat
    22681 atttaataaa gcaagaaaaa gtcattgtca attcaaaaaa aattattcca tcatatcatc
    22741 ttaaaaaagg tgatttaata tctatcattc aaattgaaga agaacaaaaa gaagaaataa
    22801 aaccagaaaa gagtaattta gaaattatat acgaggataa aaatattatt gtagtcaata
    22861 aaccagcagg aattataact cacccaacaa attataaaaa aacagggaca cttttaaatt
    22921 atgttttata ttatacttca ttggcaaaaa ttggttctcc tctaagaata ggagttgttc
    22981 atcgtcttga taaagaaaca tcgggagtaa ttgtttttgc caaaacatct cttgcttatt
    23041 ggaatcttat tgaacaattt agaaatagaa tggtggaaaa aatttattta gcaattgtta
    23101 aaggaaattt tacacctgaa aaaaaggtaa tagaattcac tgttttaccc gataaagaaa
    23161 acccaacaaa aatgaaagtc catttcctga aagggaaaaa aacaataact gaaataaatg
    23221 ctttaaaata ttttgatgat tatacacttg tctctgttaa accaattaca ggaagaactc
    23281 atcaaatacg ccttactctt tcatatttaa attatccaat tataggggat gaaaaatatg
    23341 gagtaaaatc acccattatt tcaagatgtg ctttacatgc atggaaatta actcttgcca
    23401 acccaagaga taataaaaaa caaacattta ttgcttcaat tccagatgat ataatacaaa
    23461 ttataaataa ttctaatcta taataaaaaa tttgggtata ataaaggata aataaattta
    23521 gtaaatgtag aaagtatttt aaattttttt aaaaaaatga acaataataa aattaaaaaa
    23581 ttatattttc ctgttatttt tttattaacc tgtatttttt tatttctaaa ttataaagga
    23641 tacaaatggg gagaaatttc tccaggtaaa tcagttgttc gcctatttgc agtaatattt
    23701 tcacttcttt caattttcca tattctgacc caaataataa catttgttgt aaaaaagaaa
    23761 aaagggcctc ttggtgaaac aaaaatgctt gttggttttt caaatgcaat taagggattt
    23821 gcaataattt ttgctttaat tgaaagtatg ggaaaaataa ctattttcgg tgctcttgga
    23881 gttggatttt ttggaatgct tttaggatgg tctttacaag cccctgtcag cggtattgct
    23941 gcctgggcac tgataacatt aagaagacct tttagaatag gggatagaat ttatctacct
    24001 acacttggac ttgttggaga tgttatagat attggtttaa tgtacacaat tttaaatcaa
    24061 gttggtggag cagttggaag cgaagaggca acaggacgag atgttcttat accaaatgca
    24121 atgcttttta gtcaggtcgt aataaattac actgtaaaac aggatcaacc atattttctt
    24181 gatgaagtta tttggagaat tacatttgat tctgattggg atgaagcaga aaaaatttta
    24241 attgaagcag caaaaaaagt tacagcagat attattgaaa aaacaaaggt tgaaccttat
    24301 ataagagctg atgtttatga ctatggaatt tatttgagat taagatatat aacatcggca
    24361 attgaaagac caaaaattgc atacgaaata aataaaataa tattcaaaaa agtacaggaa
    24421 aatcctaaag ttgaccttgc tattccttat acttactcgt ataaaaaggg tgtaaaaggt
    24481 aaaaataatc tttaaaattt atttatgttt tttttatcaa aaaatgataa aattgtgaaa
    24541 attaaataaa atataaaagg gaaaaatgga aaaatttgta attgaaggtg aaaataaatt
    24601 agtaggagaa attgaaatat ctggttcaaa aaattctgct cttccaatat tatcagcaac
    24661 tgtcttagga gaaggtaaat caataattca taatgttcct gatgtaaaag atgttcatac
    24721 tctcttggat atccttgaat acatgggagc aaaaacaaaa ttttataaca atacagttga
    24781 aattgacccc gatggaatag aaaaatatac cgctccttat gaacttgtaa gtacaatgag
    24841 aggttcaatt tgtttattag gcccacttct atcaaaattc aggcaggcaa gattttccat
    24901 gcctggtgga tgtgtaattg gaccaagacc tatagacctt catataaaag ggctaaaaaa
    24961 attgggggca gaaatatcta atgaagaagg atatattgtt ggaaaagcaa ataaattgaa
    25021 aggaaacaag atttttcttg ctggaaattt tggttcaagt gttcttgcaa ctgcaaatat
    25081 tcttatggct tctactcttg ctgaagatga aacaatcatt gaatttgctg catgtgagcc
    25141 agaaattgtt gaccttgcaa tgttcttaaa aaaaatggga gggaaaattt gtggagaagg
    25201 gtcacataca ataagaatag aaggagttaa aaaattaaaa ggaactgaat attcaattat
    25261 accagataga attgaaggtg gaactttatt acttttagga tatataacaa agagtaaaat
    25321 tgttataaaa aatataattc cctctcatct ttttgccctt attgataaac ttgaagaatc
    25381 agggatagaa ataaatataa aagaaaatat tgctataaca aaaccatata ataaatggaa
    25441 agcagttgat attgtgaccc ttgcctaccc tggttttcca actgacctgc aagcgcagat
    25501 tatgagtttt ctttgtcttg cagatggaat tagtataatt acagaaaagg tattcccgga
    25561 aagatttata catgcaggag aactaaaccg tttgggtgct gatatttctc ttgatggttc
    25621 aaaggcaata gtaaaaggag tagaaaaatt atatggagca aaagtaatgg cttctgattt
    25681 aagagcaagt gttgctttag ttcttgctgg acttgttgca gaaggaataa ctgaaatttc
    25741 caggatttac catattgaca gaggatatga aaaatttgaa aaaaaactaa atttattagg
    25801 ggcaaaaata aaaagggaaa aagaatgagt aaaataaaaa ttattgtatg tggtgcaaga
    25861 ggtaaaatgg gtagtttaat tttgaaactt gcaggtgaaa aagaaaattt tgaagtcact
    25921 ggtattgttg aaaacaaaaa tcatcctgat attggggaaa aaattgataa ttatattctt
    25981 tcagataatc tttcaaatat ctctactgga aaagaaatag ttattgattt tacagtttca
    26041 actgcaacaa aagaattttt aaaagtatgt gaaaataaag gtaatcctat tgttattgga
    26101 acaacaggac tttctgaaaa acaactaaat tcaattaaaa acgccgggaa aaaaatccca
    26161 gttttctttt ctcccaatat gtctcttgga gttaatcttt ttttaaaaat aatagaattt
    26221 tcatcttctc tttttaaaaa ctatgaagtg gaaataaaag aaatacatca ccattttaaa
    26281 aaagatgctc caagtggaac tgcaaaaaaa attgcagaaa taatatgtga gaaaacagga
    26341 agaaatttta aagaagatat aaaatatggt agaaatggat tgactggcga aagaaaattt
    26401 gaagaaattg ggatgcattc tttacgactt ggtgatattg tcggtgaaca ttatgtttac
    26461 tggggtggaa aaggcgaaat tattgagttt tcacataggt gttataatag agaaaatttt
    26521 gccgaaggat cattaattgc tgcggaattt ttaagtaaaa aagaaaaggg gttttataca
    26581 atggatgatt tattgaagga ggtatttaat gtttgatatt gaagatataa tttcctattc
    26641 ttcaaaatga tgttttataa ttttcccttc ttctatgaaa ataacatctt cacaaaaatt
    26701 tgttgacata tctgcaattc tttcaagtga ccttgaaatt cttataagat ggattgacct
    26761 ttcaatagtt gaagaatctt ggagcatata agtaagtaat tctctaataa tttgttcatt
    26821 taaagcatca acaatatcat ctcttttaca tatttctctt gctaattttg tatctccatt
    26881 tataaaagac aaaacacttt cttttaacat attagaaaca atctcactca tttttggtaa
    26941 atcaataagt tttttcacaa aaggtctttc aataaggaac aatgcacttt cagagatatt
    27001 aactgctgta tcaccaactc tttcaaggtc attattcatt tttaaaatca ttacaattgt
    27061 tcttaaatcc tttgcaactg gttgaaatct tgctattgtt tcaacacaaa atccatcaat
    27121 ttctacttct gccatatttg ctttattttc aagtgtatca ataacttcag aaagatactc
    27181 tttttttctt tcaataagac cttgcatact ttttcttacc atttcttctg caagtaaaga
    27241 aaaatcaatt attttttctt taagtttttg cattctttct tcaatcattt ttttctccta
    27301 tttatccaaa cttacctatc aaatactcct ctgttttttt attagaggga gcagtaaata
    27361 ttttatctgt tttaccaaat tcaatcaatt ctcctaaata caaaaaagca gtataatcag
    27421 aaattcttgc tgcttgttgc atattatgag ttacaattac aattgtatat ttttctttca
    27481 atttttctat taactcctct atttttagag ttgctattgg gtctaaactt gcacagggtt
    27541 catcaaaaag tattacttct ggttcaattg ctatagttct tgcaatacat aatctctgtt
    27601 tctgtcctcc tgataaatcc agagcatttt tacctaaatc attttttact tcatcccata
    27661 aagcagcatc tttcaatgct gtctcaactt ttaaataaag gtcattttta tttttgaaca
    27721 ttttatgaat tttaaggcca taagcaacat tttcaaatat ggattttggg aaaggattgg
    27781 gtagttgaaa aaccatacct actcttttcc gtaaaacaac ctcatccata ctataaatat
    27841 cttctccatc taacagtact tctccttcta cttttgctga atcaaccaaa tcaagcattc
    27901 tgttaattgt ttttataaaa gttgattttc cacaaccaga aggacctata attgcagtaa
    27961 ctttattttt ttcaatatca aaacttacat ttttcaatac atgtagattt gaatatctaa
    28021 catttagatt ttttataact atttttacca ttttttcttt cctcttgttt taattcttaa
    28081 aaaaattgca atagaattca ttcctaaaac cagaaataag agtactaaag cagttccata
    28141 agcaattgga acttgaaatt ctggctgtgt tccttctgtc attaaaccat aaatatgata
    28201 cggtaatgct tgaacttcat caaatataga tttaggtaat ttatttatat aaaaagtaac
    28261 tgctgtaaac aaaatagggg ctgtttctcc tgctgctcta ccaattgata aaatagcacc
    28321 tgttaaaatt ccacttattg aattaggtaa aacaatattt ttaattgttt gccattttct
    28381 tgctcccagt gaaaaagatg cttctctgaa actttgagga actgatttta atgcttcctc
    28441 tgttgacatt ataattgtag gtaatgttaa tatccctaat gttaaaccac ccgataaaat
    28501 agaaacacca aacttaaaaa gtttaacaaa tacagcaaaa ccaaaaagtc caaaaataat
    28561 agaaggaact cctgctaaat tatttatccc aattttaata attctgacaa gataattatc
    28621 tctggcatat tctgataaat atatacctgc tccaatacct aaaattaagg aaaatataat
    28681 tgaaacacct actaaataaa ttgttcctaa aattgcgggg aaaattcctc cttttgtcat
    28741 tccttctttt ggcatactaa aaagaaactc taaatttatt accttaaaac ctcttataaa
    28801 gataaagaaa aaaattaaaa agaaaaaaat taaaattaaa atattacaaa ttctaaatac
    28861 ccagaaccat aaattttgtt ttaatttttt catcttactc tctttttgaa tctttcatat
    28921 aaaaaatcag ataatatgtt aaaaaataaa gtaattacaa aaagaacaat accaagagaa
    28981 aacagagcat tgtaatgaat tcctcctaaa actgcttctc ccatttcagc agcaattgtt
    29041 gctgttaaag gtctaactgg ttctaaaaaa gaatgaggga taattgctgc tcctcctgct
    29101 accattaaaa cagtcattgt ctctccaatt gctcttgata ttcctaatat tattgcagta
    29161 attattcctc cacttgatgc aggaagaact actttaaaaa ttgtttctaa ttttgttgca
    29221 cctaaggcaa gtgatgcctc tttgtaatta tttgggacag atgatattgc atcatctgca
    29281 atacttgtaa tagttggaat agccataatt cctaaaatta tagaagcagt taaagcacat
    29341 aatccaacag gtaagtcaaa aattttctgg ataaatgggg ctaaaattac tacaccaaaa
    29401 aaaccataaa caacagaagg tattcctgct aatagttcaa ttattggttt tatgatttct
    29461 tttgtcgttc taccagcaat ttcagaaaga taaatagacg ctcctattcc taatggaatt
    29521 gccaatatta gcgcgcctaa ggttacccat aaagaaccta aaattaaagg tagtgcacca
    29581 aaacttggtg gttcataagt tggataccac tcttttccaa ataaaaagtc cttcaatgaa
    29641 acatatttaa ataggggtaa tccctctttg aaaagaacta caactatacc taataagaaa
    29701 aataatgaaa gaaacgaaag aaaaaataaa atatatttaa taaaattttc ttttaatttt
    29761 gtcattttta ttccttatat taccataaat aaacctgtta tatcaaataa acaccccctc
    29821 aaattgaggg ggtgtttata ataaaaagga tattctttat tccaaaggaa caaatccttc
    29881 ttctttcaca atttcctgtc ctttttttga ctttacaaaa tcaatgaaat ttttaaccag
    29941 tcccttaggt gttccatttg tgtacataaa taaaactctt gatattggat atgttccatt
    30001 tagaactgtc tcttttgaag gataaattcc attcactttt actgctttga catcagaacc
    30061 caaataacca agtcctacat atccaatagc accttctgtt cttttaacaa tatttacaat
    30121 tgcctgattt gatgcttgca taagagcatc tggttttact ttttcattct ttaacacaat
    30181 ttcattaaaa cactcaaaag ttcctgaggc agtatctctt gaaacaacca ctatcttctg
    30241 gttttcccca cctaattcct tccaattaga tatttttcct gtgtaaatat ctttaatttg
    30301 agaaagagtt aaatcactaa taggattaga tggattgaca attactgaaa ttgcatcgtt
    30361 agcaattaca tttgctttag ggtctatacc ttttgatatt gcagttttta attcctcttt
    30421 tttcattggt cttgaggaat tagcaatatc acacacctta tcaatcaaag cagaaatccc
    30481 tactccagaa ccaccacctc ttacagaaat acttactcct ggattttctt taataaattc
    30541 ttctgcacat ctttgggcaa caggaaaaac agtagtagaa ccctgtaaaa ttatactttt
    30601 ttcctgcgcg ataacaaaag atgttagaca taatgttaga accagatata aaaaaatttt
    30661 tttcattttt cctcctttta aaagttcact acataatcaa tttgaagtgt atttacatca
    30721 cctctgccta cataattacc ttcaccgaca atatatgtta tacctgcagt ggagttatca
    30781 tttattgcat acttaacccc aaatttatgc ccttttgaac cagttccacc gccattgaaa
    30841 tcagaatcat ttaagaaagc aggaacacaa tctctctcaa tatctctgta gttatagtca
    30901 aattcccatg ttcctttttt cttcgctttc ccaactgtaa acccaatttc atagccagtg
    30961 tctttatcaa attcactttc agtgtttttt acataatcag cataaagttt tacaggtaca
    31021 ccctgaattt caaagggata aaactcaaaa acaacatcaa ttggtttata ttcagcaata
    31081 taatttccac ctattattgt attacctgta cctgcactac cagcacctac aacacttgca
    31141 gttgttcttc cttcaattcc ttctgtcata taataagcaa ctgctatttt aaagtccctt
    31201 gatgcaattt tccctgaata tccaacttga atacctgtca tgtatgggtc gctttcccat
    31261 ccaccatctt cttgtaatgg aaaaaatcca ccatttataa aaacaccaac agggaatttt
    31321 gtttgaaagg caataccttc aggagttata tccccatccc atacaaggtc tgtgttaata
    31381 aaaggatttg aaaattttcc accaaaaatt gatatatatt tattaaagga atattttata
    31441 tatgctttgt caagccagat gttttttgat gaaaagacat cttctaatgt ctgatttgtt
    31501 gatttttggt ctgatgaacc agttgcaact cttactccta tctctgtatt ctcatttggc
    31561 tttgtttcaa atccatatct cagtcgtatt cttcctctat gtctatcaac agggtcttta
    31621 tcttccattt cgtatcttaa tcttgcatct ccactaaatt tcattgtatc aagccatttt
    31681 ggaacttctc tttcttttgc ttcttctttt ctgacttctt cctgtgtttc tacaagaatt
    31741 gtctgcgctt cacctgaagt aaggattcct ttttctacaa gtttctgtat aagaaggtct
    31801 acatcagaag caaaggaact aactaaaaaa gataggaagc atactatccc tgcaagtagt
    31861 acttttcttt tcatttttcc tccttttttt gttgtttttt ctaaactttt atttttatat
    31921 tcatctttat ctaataaacc ttcttctctt gatgcctttt tatttttttt cacctccttt
    31981 ttggcatttt tttcaataga agaagatttg aagagtttta aaagcatatt ttttataatt
    32041 tctggatatg tcccgatttt cctttttgaa agatatgaaa ttgttaattt caacaaaaaa
    32101 gccagaagta aaatcaaaaa aaatagaata attacatttc tgcccattgt ggtcctcctt
    32161 taatccctat tttcttatcc tctttttctg taaataaaac tacatctcct ctttcagtaa
    32221 gttcaacttt tacatttttc cactcaaaat caggagaaag ttcagttata taagaaatag
    32281 tttgccatgg gaaaattctt gtttttattt cgctggaata tattccaaaa ctataagtgg
    32341 gttttttaaa atggaatgtt ctacatctca tttttatgta tatttgattt tcttctaata
    32401 cctctccaac tgctatgtga tttgttgcat cctgatatgc cttttttatt cttactttcc
    32461 atatttttct catttgtttt tccattttca ccaaataatt ttaatgagaa aaagttaaga
    32521 aagagtaaag aaatggtaaa aatttggtaa aaaatgatat aactatatag gaaggttaat
    32581 tataaattca gttccagaac caacctgact tttaacttct attttcccac taagagaaat
    32641 aactgcattt tttacaattg ccaacccaag acctgtccca ccagtttctt ttgaccttga
    32701 tttatcaaca acataaaatc tttcaaaaat tctttcaata tctttttcag gaattccaat
    32761 acctgtatct ttaatagata taaaaaggtt atttcctaat accttacttt tgattgtaat
    32821 ttccccattg tcagtatatt taatagcatt atctataaga ttaaataata tactttcaat
    32881 taataaccta tctgtcatta aactattttt aatttccata tctactttta cttttatttc
    32941 tttctccttt attttttttt caaaaatctt tacaatatca tctgttatct cttttaaatt
    33001 aacattttct ttctgaattg ctttttcttc aatataagaa agacataaaa tatcatctaa
    33061 tatatttatt aatctatctg tgtttttcat tataattttt atattctctt ttgtatcccc
    33121 ttctacttca tcataaattg tttcaagata ccctttaata actgttaatg gtgttttaat
    33181 ttcatgagaa atatttgcaa caagttcttt ttttatattt tttagttggt atgtctctgt
    33241 tatatctaag aaatgaacag cgactgtgtt atcaaaaaac ttgaaaacat gacaattaaa
    33301 aattttatta ccaatttcaa tttcactttg tctgaattct ttatcattta atatatcttt
    33361 aatacactca aataaattat aattatcaaa aaattcaaga taatttttcc caataaattt
    33421 ttcttcatcc ctatttgaaa aaacaatttt attctcttta tctattataa aaatagttgt
    33481 tggaagaaat ttaaaaaaag aataaatttt ttctttcatt tcttttattg ttttttcttt
    33541 tttctctatt tcttccataa tttcttcttc tgttttctga ataaaaacta tatctgattc
    33601 tttttgataa acatcttttt tatactttct tagattttct tttaaatttt ttataggcag
    33661 gtaatttaaa aaagaaataa gaaaaataaa aattaaacaa ataaatatag gtaaagaata
    33721 aattttgtta aaaagaataa aaaaaataag taataatata tttgaaataa gtaatcggga
    33781 aaaataattt tttatcattc ttctatttta tacccaactc ctcttattgt ttttatatac
    33841 ttaccatact tgcctaattt tgcacgaaga ttgtttatat gaacatctat tgctttttct
    33901 attgtaaatt tttccccatc ccataaaagt tctattatct tgtctcttgg aaaaacccat
    33961 gattttttag aagcaaggat taaaagtatt ttaaattcaa cataagttag attaattctc
    34021 ttgttattaa ctaaaacttc aaatgttttt gggtcaataa taatttcttt cccaatatat
    34081 atcttttcag ttgataactc caacttatat cttcttaaaa cagcccttat tcttgctaat
    34141 aattctttta gagaaaacgg ttttgttata taatcatctg caccaatttc aagtcctaat
    34201 attttatctc cttcatccgt tcttgcagtg agcattatga caggaatttt ttctgttttt
    34261 ggatttgttt ttatgttctt acatacatca ataccatcca tatcaggcag cataaggtca
    34321 agaagaacaa ggtcaaactt ctctttctct atatttttta aaaaatcatt tgcgtttgta
    34381 taaggaaaag gtaaaaaatt atttttttct aaatactttt tcataagatt taaaatatca
    34441 atttcatctt caactattgc tattctttca ttcatatttg ttttctctat atcagttttc
    34501 taattcctat attttggttt atatctttat agtacagttt gaaaagttat cccttttatt
    34561 tattatctta taatataaat tataaaacat ctaattttct taattgttta ttcataaaac
    34621 cttattgtca actttattgt tctattatat aataatgtta ataataattg aaggaggtat
    34681 ttaatgtttg atataagcga aagaatgaag aaaataccac catacctttt tgtagaaatt
    34741 gataaaaaga aaaaaaaact aataaaagat ggatatgatt taattgactt cggaattgga
    34801 gaccctgata tacctactcc atccaatatt gttagtgcta tggcggaata tattaaaaaa
    34861 cccgaatttc atagatatcc attaggaagg ggaaatatta attttagaaa aagtatatca
    34921 tctttttatc aagaaaatta tgatgtccaa ttagaccctg aagaagaaat atgtgtttta
    34981 attggctcaa aagagggaat tgctcatttt ccactttgtt ttataaatcc agatgactat
    35041 tctcttatac ctgaacctgg atatcctgtt tataaaattg gaacaatttt agcagatggg
    35101 aattgttatt ttctaccatt aaaagaagaa aataattttc ttcctgactt ctcaaaaata
    35161 cctgctaata ttttaaaaaa agcaaaaata ctctatctaa attatccaaa taatcctact
    35221 tctgagtttg catcagcaga atttttaaaa gaagcaatta acttttgtaa aaaatataat
    35281 ttaatccttg tttatgatgc tgcatatagt gaaatttatt atgatgaaag acctgttagt
    35341 ttcctttccc ttccaggggc aaaagatgtt ggaatagagt ttaattcatt atcaaaaaca
    35401 tttaatatga ctggatggag aattggatgg gcatgcggga ataaagaact tatttcagca
    35461 cttagtaaag taaaagaaaa tattgattca ggtgtttttg aagcaattca atttgcagga
    35521 atagaagcac taacaaatag caaagagaca cctttgaaga tgcgagatat ttataaaaaa
    35581 aggatgaatt tatttgtcaa tggtttaaaa gaagttggtt ttgatgttaa atttccaaaa
    35641 ggaacttttt atctctggct gaaagtaaat aaaagttcag ttgaatttgt tgacgaactt
    35701 cttgaaaatg gtaaaataat tgcaactcct ggaattggtt ttggaccaag tggggagaac
    35761 tttgtgagat tttctcttac aattcaggaa aacaaaataa tagaagcaat tgaaaggatt
    35821 aaaaaaatat gggaaaagta gcaatagttg taggaagcaa aaatgattta gagttaattg
    35881 aaggagcaaa agaaattctt aaaaattttg aagttgaatt tgatgttttt gttctttctg
    35941 ctcatagaac ccttgatgat acaatttctt ttgctgaaaa tgcagaaaag aaaggatatg
    36001 aagtaattat tgctgctgct ggaatgtcag cacatttacc tggaataatt tctgcaaaaa
    36061 caattctgcc ggtaattggt gtccctcttc caacaagtga aattaaagga atggatgctt
    36121 tactttcaat tgttcagatg ccaacaggag ttccagttgc gacaatgtcc ataggaaaag
    36181 caggagttaa aaatgccgct ttattttcaa ttgaaatact tgcaagaaat gacgaaaagt
    36241 taaaagaaaa gttaattgaa tataagaaaa acctaaaata aagaacttat aaatattagt
    36301 aaatactcag tttagatgat atatgataca atctattaca aaagaacaaa accaaaaaag
    36361 gaggataaaa tggctgtgag ttataaggaa cttggacttg ttaatacaca agaacttttt
    36421 aaaaaagcat tggcaggtgg ttatgcagtt cctgcctata attttaacaa tatggaacaa
    36481 ctacaggcaa taattactgc atgtgcagaa tgtaattcac ctgtgatact tcaaatatct
    36541 aaaggtgccc ggcagtacgc aaatcagaca ctattaaggt atatggtgcc aggagcagtt
    36601 gctatggcaa gagaaatggg cagtaatatc cccattgcat taaatcttga ccacggcgac
    36661 tcttttgaac tctgtaaaag ttgtattgac tatggttttt ccaatgtaat gatagacggt
    36721 tctgcacttc cctatgaaga aaatgtgtct attactaaaa aggtagttga atatgcacat
    36781 gaaagagatg taaccgttga aggtgaactt ggtgttcttg ccggtataga agagcatgtt
    36841 tcttctgaag tcagtcacta tacaaatcct aaggatgttg aggatttcgt aaaaagaaca
    36901 ggggtagata gtttagcaat atccattggg acttcacatg gtgcttataa attcaaggtg
    36961 aagccaggcg aacctctgcc acctttaaga tttgatatac ttgaagaatg tgcaaagaga
    37021 cttccatcat tccctattgt tcttcatggt gcttcttctg tacttaaaga atatgttgat
    37081 attatcaaca ggtatggtgg aaaacttgaa aatactgctg gtgttcctga agaccagata
    37141 aggaaagcag ttacaatgaa tgtttgcaaa gtaaatattg actctgacgg tcgccttgta
    37201 atgacagcaa tgataagaaa atatcttgct gaacatccag aggaatttga ccccagaaaa
    37261 tatctcggtc ctgcaaggga agaattgaaa aagatgtata ttgcaaaatg taaccttctt
    37321 ggcagtgctg gtaaaggaca ataataagtt aaaaagcaat acttataaac caacaattgt
    37381 aaattaaaat gaaaaaaatt gctgttgtta ctactggtgg agatgcacca ggtatgaacc
    37441 ctgctataag gtcagtcgtc agaactgctc tttataaaaa catctctatt tcggggataa
    37501 gaaggggatt tttgggccta tatgaaggtg attttatacc tttaagttca agagatgtcg
    37561 gtaatattat aaatagaggc ggaacaatcc tcaaaacagc gagattccct gaatttaaag
    37621 accataatat aagaaaaaag tgccttgaaa aattaaaaaa agaaaaaatt gatgggattg
    37681 taataatagg tggagatggt tctgcaaatg cggcatactc aatttataat gaattctcat
    37741 atcctgttgt tcatattcct gcttcaatag ataatgacct ttatggaact gattggacaa
    37801 ttggatttga cactgctgtt aacactgcgg tagaagcaat tgataaaata agggacactg
    37861 ctacaagtca tgaaaggaca tttatagttg aagtaatggg aagagaaaaa ggtaaccttg
    37921 ctgttgaagt tgcagtcgcc tgtggtgctg aaaatgtaat aataccagaa attgaattta
    37981 atttagacca acttgtttct tctctaaaac agcaacagga aaaagggaaa aatagtgctt
    38041 taataattct tgcagaaggg gcgggaaaag cagaggaatt agcaaagcaa ctgacagaaa
    38101 aattacctga tagagaaata agatattctg ttttaggata tattcaaaga ggcggaagtc
    38161 cgacatattt aacaagaaca cttgcaaccg tttttggatg gtatgcagta aaatttttaa
    38221 ttgatggtga ctatggatat atggtaggca ttgtaggaaa taaaattgag aaagtccctc
    38281 ttgagaaagt tgtttctaat agaaaattac ctgatatgga aaaacttgag acaattaaat
    38341 atatggcaat ttaatttgaa aatacccagt tctgttggaa ctgaaaagta gcgagtagag
    38401 agtagcaagt ataaatggaa aagtaataaa aattatagaa aaaaaaatgt ccaaagaatt
    38461 ctgtagggaa gaaaaaaaga aaataaaaga attattcaaa gcaaaaaaat tatttcataa
    38521 ggaaatggcg aaaattcctt ttgaagaaaa aattaaaata gttgttagat tacaaaatat
    38581 agttaatgaa ttgaaaacaa ataaaggaag aagaaaaaaa agagtatgga aaatttaatt
    38641 ttaaggagaa ggaaatgaga agcgataata ttaaaaaggg catagaaaaa acacctcata
    38701 gaagtttatt aaaagcagat ggttttacag atgaagagat aaaaaaacct attattggta
    38761 ttgcaaattc cgctaatgaa attatacctg gtcatattca cctcaacaaa attgctgaaa
    38821 gtgtaaaaac aggtatcgtg tctgcgggag gcacccctgt tgaatttggt gttataggtg
    38881 tctgtgatgg gattgctatg ggacaccttg gaatgaaata tagtttggtt tcaagagaag
    38941 taattgcaga tagtgtagaa attatggcac aagcacacca atttgatgga atagttttag
    39001 ttgcaagttg cgataaaatt gtgccaggaa tgcttatggc tcttttacga ctcaatattc
    39061 caggtatttt gataactggt ggtcctatgt tgtgtggata tttaccaact ggcgaaacaa
    39121 tagattttat ctctgtttct gaaggagttg gaaaattttt aaaaggtgaa attgatgaaa
    39181 agcaattaaa aatattagaa gatgaaggtt gtccaggagc aggcagttgt gcaggaatgt
    39241 ttactgcaaa ttccatggga tgtttatcag aaggacttgg actttcactc cctggaaatg
    39301 gaacaattcc tgctgttagt tcaaaaagaa ttcgtttggc aaaatatgct ggaataagag
    39361 cagttgaact tgttaaaaaa gatattaaac catcagatat tatttcaatt gaaagtttta
    39421 aaaatgcaat aactcttgat atggcaattg gtgcttcaac aaatactgtt ttacatcttc
    39481 cagcaattgc aaatgaaata ggtattaaac ttaaccttga aatttttgat gaaataagta
    39541 aaaagacacc aaatatatgt aaattatctc ctgcttcatc ccaacacata caggaccttg
    39601 atagagcagg ggggatacca gcagttatga aggaattatc aaaaaatgga cttattgaaa
    39661 aaaattgttt aactgtttct ggaaaaacca taggggaaat aattgaaaaa gcaaatgttt
    39721 gggatagaaa tgttataaga gacataaata atccatattt acaagaaggt ggaattgcta
    39781 ttttaaaagg aacacttgca cctgatggag cagtaataaa acaatcagca gttaaagaaa
    39841 atttattaaa atttaaaggg aatgcaattg tttttgattc agaagaagaa gcaatgaaaa
    39901 gtttagttga agggaaaatt aaagaaggag ttgtaataat aaggtatgaa ggaccaaaag
    39961 gtggaccggg aatgagagaa atgctttcaa tgactgctgt tattgcagga aaagggattg
    40021 atgagaaaat tgccttaata acagatggca gattttcagg aggaacaaga ggactttgta
    40081 taggacatgt aagccctgaa gcagaagagg atggtcctat tggatatgtt gaaaatggag
    40141 atataattga gatagatgtt ccaaaaagaa gattggatat aatggtaaaa ccagaagagt
    40201 taaaaaatag aaaaaggaag aaaaaagaga aaaaattaaa aggaatttta gcaagatatt
    40261 caaagcaggt aaggtcagca aatacaggag cgatattaga ataaaaacaa attctcatta
    40321 aaaatcaatt aaaacaaagg aggaaaaatg gaagatataa gaaaaggaaa gggtgattat
    40381 gaatggttta ttaaagcaag atttggtctt ttcattcatt ggggacttta ttcaatgccg
    40441 gcgagacgag aatgggtcag aagttatgag gaaataccaa atgaaatata tgatgaaaaa
    40501 tattttaaaa gattttatcc agacctttat aatccaaaag aatgggcaaa aatagcaaaa
    40561 aatgcaggaa tgaaatatgc agtgataaca acaaaacatc acgaaggatt ttgtttatgg
    40621 gatagtaaat taactgatta taaagcaaca aatactccta ttaaaaaaga cctattaaga
    40681 gaatttgtta atgcctttag agaagaaggg attagaattg gattttatta ttcactgatt
    40741 gactggcatc atcctcatta tacaattgat atgtatcatc cattaaaaaa tcatccagaa
    40801 aaagagaaat taaataaaga aagagatatg tctatttaca gaaaatattt acatggacag
    40861 ataaaagaac ttttaactga atatggaaaa attgatttat tattttgtga tttcagtttt
    40921 ccaggacccg atggaaaagg aaaagatgac tgggatagtg aaaatcttgt aaaaatgata
    40981 cgggaattac aaccacatat attgataaat gaccgacttg accttcctga tgcatgggat
    41041 tttaaaacac ctgaacaggc agttccttat gaatggccag aagttaatgg caaaaaagta
    41101 atctgggagt catgtcaaac attttcaagt atatcatggg gatataacag agatgatata
    41161 tggagagata gcgaagaaat tacaaaaaca ctcattgatt gtgtaagtaa aggaggaaat
    41221 ttacttttaa atgttggacc aactgcaaga ggtgattttg attataagtc aagaaaaaga
    41281 ttggaagaaa ttggtaattg gatgaagtat aattcaaggg caatttataa ttgtactcaa
    41341 gcacctaagg aatttaatat ccctaaggat tgtagattaa catataatcc agaaaaagga
    41401 actctctata ttcatttatt ttcttaccct tacaaatttt tatttttaga aggaaaagaa
    41461 tggaaagaaa atggctctta tgcacaactc ttaaatgatg gcagcgaaat aaaaatagga
    41521 cttgatgaat ggtataaaaa tcaattaggg gataaagaag gaattgttct atgtttaccg
    41581 ccaattaaac caaatgtttc tgttccagta attgagttat ttttaaaata aatatcaaac
    41641 aatgaataaa ataaaatgga aaaagaactt gcaaaaaaag gtattataag taaaggtgta
    41701 aaatttattt cagaaaaaga aaatgtaaat cccgaaatta ttgctaaaaa cattgcagaa
    41761 gggacatgtg taattttaaa acataaggat acatatttag gaattgggaa ggacttaaaa
    41821 acaaaaataa atacaaatat tggaacttct cctgatatat ttgatttaaa cttagaaatt
    41881 caaaaattaa aatatgcaat taaatatgga actgatactt taatggattt aagcacaggt
    41941 ggagacctta ataaaataag aaaagaaata ataaaagaaa gttcagttcc aattggaaca
    42001 gttccaatat atcaatcagc aattgaagta gcagaagaaa aaggcggaat aacaaaaatg
    42061 gatgcggata aaattttcca aataattgaa caacatgcga aagatggggt aagttttatg
    42121 actgttcatt gcggtataac tttaaataca cttcaacgac tgaaaaaaaa tccaagaaaa
    42181 acgcttgttg taagtagagg aggggctttt cttatttcat ggatgatatc taatgaaaaa
    42241 gaaaaccctt tatataaaaa ttttgaccga ttacttgaaa ttgctaaaaa gtatgatgtt
    42301 gttttaagtt taggagatgc aatgaggcca ggaggtatta ttgattctac tgatattgct
    42361 caaattgatg aactcttaaa tttaggagaa cttacaagat atgctgaaga agaaaatgta
    42421 caggtaataa ttgaaggacc tggtcatatc ccaattgatg aaatagaaac aaatgtaaaa
    42481 ttagagaaaa aaatatgtaa aggagctccg ttttatgttc ttggacctat cgtgactgat
    42541 attgctcctg ggtatgacca tataacaggt gcaattggtg cttctattgc tggttggtgt
    42601 ggtgctgatt ttttatgtta tgttacttct gctgagcata tttgtctacc tggattagat
    42661 gaagtaatag atggagttat atcttcaaaa attgctgctc atgttgcaga tataggaaag
    42721 ggaataaagg gaacaaagga aattgattat aaaatggata tgtacagaac tgatagaaat
    42781 tgggaaaaag aaaaagaaat atcttttttc ccagataaat ttgagcaatt acacaaaaaa
    42841 tatgagaaaa ccgaaagaga tgtttgtaca atgtgtggaa aatactgtgc aattaagtta
    42901 gtagaacaat atcttggctc caaaatcata ttttaaaatg agaaaaataa gaaaatatgg
    42961 agaagaaata ttaagaaaaa aaagtaaaga agtagaaaaa atagatacta atttgttaaa
    43021 attgattgaa agtatgaaac aaacattggt ttattataaa gggttaggac ttgctgcctg
    43081 tcaggttggt gttttaaaaa gggtaattgt tgctttagac caaaaaacaa aagaaataat
    43141 aactgttata aatcctgaaa ttgttgaaac aagtgggcag gatattgata tggaaggatg
    43201 tttaagtttc cctgaaattt atttctctat aaaaagaccc aaaaaagtaa ctttaaaagg
    43261 aataaatgaa aagggaaaag aatttattta tgaaggtagt ggagtattag gtagatgttt
    43321 ctgtcatgaa gtagaccatt taaatagtat tttaattatt gattatgcaa ctgaagagga
    43381 aagaaactat tggaaagaaa aaatagataa aataaaaaat aaataaaagt gtaaaattca
    43441 aaatgtaaag cgaaaaataa aaatgtaaaa aagaaacaaa aagagttaac tttttaaact
    43501 ttaagttgta attttacatc ctacaaaagg catcggggga taatagaaaa ctcgttttta
    43561 atttttatat caattatttt ctaaaaggat atatggaaaa tgatacctga ttatcttgtt
    43621 tcatttgata cagaaaaatt atcaattgaa gaagtagatt ttcttataat tggtggtgga
    43681 gttgcaggat taagggctac ccttgaaata aaaaattata aaacacttat tgtttttaaa
    43741 aatggtattg aagagtcatc atcttttaat gctcagggag gaattgcaag tgcaattgct
    43801 cctggggata aagatgaata ccatataaat gatactttga aaactggctg tggaattgca
    43861 gataaacaag cagttgaagt gttagtaaaa gaagggaaag aaagaattaa agaattgata
    43921 gaaatgggat ttaaatttga taaaattaat ggaaaattcc attttactct tgaaggtgga
    43981 cattctgtgc caagaatttt acattcaaat ggcgatttta caggacagac attaacaagt
    44041 tttttatatg aaaaagtcct ttcaatgcct aacataaaaa ttattcattc ttttttttta
    44101 atagacctta ttgttgaaga aaataaggtc tatggaggaa tttttttaaa tacagaaaat
    44161 aaaaaattat ttattataaa agcaaaagca gttattttag caacaggagg agcaggaaat
    44221 gtttatcagg aaacaacaaa tccatcaagt ataacaggtg atggaatagc aattgcatat
    44281 agaagtggag cagttttatc tgatatggaa tttgtccaat ttcatccaac tacactttac
    44341 attgcaggtg ctccaagatt tctaatttcg gaaagtgtcc gaggagaagg gggaatatta
    44401 agaaatatta atggtgaaag gtttatgttt aaatatgata aaaatggaga actcgctcca
    44461 agagatattg tatcaagagc aataatagat gaaataaaaa ggacctcttc aagttgtgtt
    44521 tatttagata taacacatct taaaaaatca tacattaaaa aaagattccc tcatattttc
    44581 aaattctgcc tttcatatgg acttgatatt acaaaaaatc ttattccagt caggccttct
    44641 gcacattatt ttattggtgg aataaaaaca aatttatggg gacaaacaaa tattaaaaac
    44701 ttttttgcgt gtggagaaac agcatccaca ggagtccacg gagcaaatcg gcttgccagt
    44761 aattctctcc ttgaaggact tgttttcgga gcaaggtgtg ggaaaaaaat aattgaagat
    44821 tttagcaata taaaaattca aaatattaaa caaaaatata aattcccaca aaaaggagat
    44881 attttaattg acagatatga ccttaaaagg tctataaaaa gtttaatgtg gagacaggta
    44941 gggattgaaa gaaatggaga taaattaaaa gatgccaata atagattaaa agaatgggaa
    45001 aaatatgctt ttctaaaaga gttttatgat aaagatgggt ttgaagcaca aaatatgtta
    45061 attgtctcaa aattgattgc ccattcttct ttaaaaagga aagaaagtag aggaactcat
    45121 tttcgttttg atttcccgtg tactgatgac agatgggaaa aacacatact tattaagaaa
    45181 aataaataat cagaaaaaaa attaaaaagt gtaaaatgta aagagtaaag tgtaaaacga
    45241 aaatataaaa cgcgtatttt agcgtccagc tatgtagtgt ggcggattca aaacgaagaa
    45301 gaacaaaaac ccaatacttt ttacattttt aattttacac tttcaacttt aaatttataa
    45361 attgttcttc tatatgccca ttataataat ctttattaag tttttatata aaaaatgata
    45421 aaagatagaa taaaagaaaa aaaacaaaaa cctgaagaaa gaaaaagaaa ttttaatgaa
    45481 gtttcttttg gttatacaaa ggaagaggca gttgaagaaa gtaaaagatg tcttcaatgt
    45541 ccaaaaggtc cctgtgtgca gggatgtcct gttggaatta acatacctgc ctttataaaa
    45601 gaattacaag aagggaatat tcaaaaggca cttgatataa tttatgaaaa aaacttactt
    45661 cccgctattt gtggaagagt ttgtcctcag gaaacacaat gtgaattatt ttgtagttta
    45721 aataaagcaa aaaaaccagt tgctattgga aaacttgaaa gatttgctgc tgattacgga
    45781 caacagatat acaaatgtaa tactgaaaaa atatcagaaa ttgataaaaa taaaattgca
    45841 gtaattggtt ctggtcctgc tggattaaca tgtgcggctg aacttgcaaa atgtgggatt
    45901 aaagtaacaa tatttgaagg attgcattta ccgggaggag ttctttcata tggaatccct
    45961 gagtttcgtc ttccaaaatc aattgttttt aaagaaattg aatttctcaa aaagatagga
    46021 gttgaaataa aaacagatat aattgttgga aaaacaatta ccattgaaga catttttaat
    46081 gacgagtata gagcaatttt tattggcagt ggtgctggtc ttccttcatt tatgggtata
    46141 tcaggagaaa atttattagg tatctattcg gcaaatgaat ttttaacaag agtgaatctt
    46201 atgaaggcat acaattttcc tgaatatgat actcctgttt ttattggtaa aagagttgct
    46261 gttattggag gaggaaatgt agcaatggat tctgcaagaa gttcattaag attaggagca
    46321 aatgaggtat ttatacttta tagaagaacc gaaaaagaaa tgccagcaag aaaagaagaa
    46381 tatgaaaatg ctgttgagga aggtattaaa tttacctttt tagtccaacc acttgaaata
    46441 atcggagatg aaaatggaca tgtaaaaggg ataaaagtta tacataatga attggggaaa
    46501 ccagatgaaa gtggtagaag aagaccggtt ccaattgaag ggactgaaga aattttatct
    46561 tttgacacaa ttattgttgc aataggtcaa aatccaagtc cattaattcc aaaaagttat
    46621 gaaaaactaa aaacaggtaa aaaaggaaat atagaagtaa atccagagac atgtgaaaca
    46681 aacatacctg gaatttttgc aggaggagat atagcaacag gagcagcaac agtaataagt
    46741 gcaatgggaa tggggaaaac agcagcaaag aatataattg aaagaataaa aaaggggcaa
    46801 taatatgaaa ataaaaatgt caatttgtaa atggacttat agaaatagag gcattttagt
    46861 tagtcctcca ctattatttg caattttttc tttttttgat gagattgaag ttgattattt
    46921 aatctggcca cttggtattt caatttttct tttaggactt ttacttcgca tctgggcaca
    46981 ggaacatata cattatcgtt tgaaaacaga aggtcattta actaccacag gcccatatac
    47041 ttctatcaga aatcctattt atttaggtaa tactttaatt tgtgtcagtg caactatttt
    47101 atcagaactt ttatggcttg ttcctattac aattatatgg tgtggaattg tttattccct
    47161 tgttgttaga tatgaagaag aaaatttatt agaattgtat ggagaacaat atcataaata
    47221 tatgtcagaa gttccacgat ggttccctca ctcatttaaa aacgctaaaa atttgatgtt
    47281 taaaaatgaa tatctaagtg cttccttaat tgcagaaatt caatgtctat ttgctttatt
    47341 gccaccaatt ttaaaggaaa taatatcttt ctggataaat cattaattat ttcttttttc
    47401 tgttcatacc atataatatc tttaattgac ctgaaataat ctctatgata aaatcaagtc
    47461 aatagaaatt taaccttaaa aaggaggaaa tgatgggatg gataatttta ggtgtttttg
    47521 tatttatttt gcttgcgaat tcaattaaag ttgttaatga atatgaaaga ggagttattt
    47581 ttcgtcttgg acgacttgtt ggagcaagag ggcctggtct ttttttcctt attcctatta
    47641 ttgaaagaat ggtaaaaatt agtttgagaa taataacatt tgatgtaact ccacaggaag
    47701 ttatgacaaa agacaatgtt ccaataaaag taaatgcagt tgtttatttc agagttatgg
    47761 acccaaataa ggcaattgta gaagttgaaa attatcaaat ggcaacaatg cagatagcac
    47821 aaacaacttt aagaggaatt gtaggacaac acgagttaga tgaaattctt gcagaaaggg
    47881 aaaaaataaa tcaacgacta caacagataa tagatgaaca aacagaccca tggggaataa
    47941 aagtaagtat tgtagaaata aaagaagtag aacttcctga tactatgaaa agagcaatgg
    48001 caagacaggc agaagttgaa agagaaagaa gagcaagagt tataaacgca gaaggtgaat
    48061 tacaggcatc agaaaaacta aaacaggcag cagaagtttt atctcaaagt ccagtggcag
    48121 tgcagttaag gtttttccag acagcatcag aaatagcagc agaaaaaaat tctacccttg
    48181 tttttccaat accacttgaa tttctaaagt atcttgaaaa aaagaatgaa tgactttaaa
    48241 aaaataggca ttgttgggtt aggacttatt ggtggttcta ttggcaagac aataaaaaag
    48301 aaaagtagta agataactgt ctatggaatt agtagaagac aagcaactct aatcaaagca
    48361 aaagaagaga atataattga tgaatatttt ttaaattttg atgaaattgt agaaaattct
    48421 gaacttttgg ttctttgcac tccaatagat aaaattgaat attactttag aaaaataaaa
    48481 cagacaggga aaaatatata tcttacagat gttgcaagtg taaaaaaaag aattgtagaa
    48541 aaaggagaaa aaattttagg cccattattc ccatatgtcg gttctcaccc aatggctggt
    48601 tcagaagaaa caggactttt tgcttcatct gataatttat ttgaaggaaa aaatgtaata
    48661 attactccaa caaaatcatc agataaaaat gtcgttaaga aaattgaaaa tttctggcaa
    48721 tttcttggtg caaatacatt ttttcttact ccatctgaac atgataaatt tgtttcccta
    48781 acgagtcatc ttccgcattt ccttgttttt cagttcttaa aaataatcag tcaggaaaaa
    48841 gaaaaaaaga aaatagaaaa atgtattggt cctggttttt tagatagcac aagaatagga
    48901 aaaagcaatg ttgaaatgtg gacagaaatt tttctgaata ataaagaaaa catattgagg
    48961 ggtatatcaa aacttcaaaa ggaaattgag aaaacaaaaa aatatttaaa agcaaataac
    49021 aaagagaaaa tacttaaaga atttgaaaag ggaaataaat ggagaaactc aattgaaaaa
    49081 aaatgacctt ataaaaaaag caaaaagaat agttataaaa attggaacta atgttttaac
    49141 aacacaaaca aatcgtttag atacttcaat aattgaacat cttgttgaac aaattacata
    49201 tttaattgag aaaaaagaaa aagaaataat aatagtaact tctggggcaa ttttatccgg
    49261 tatgcaggtt ttaaattggg aggaaaaacc aaaacaaata aatgaacttc aagcagttgc
    49321 aagtattgga cagggtaaat taatgggtgc ctatgaaaga atttttaagg aagagggtat
    49381 aaatgttgga caaattcttc ttacaaggga tatttttatg agtaagaaaa gggcaaaaat
    49441 tgcaagagaa acaatattaa cacttttgaa acataaaatt gtccctataa taaatgaaaa
    49501 tgatagtgtt gcttttgaag acataaaatt tggagataat gatatactat ctgcttatgt
    49561 tacaaactta attgaagcag atattctaat tcttataaca gatgttgatg ggctttataa
    49621 aaattattca gataaaaaag gtggagtaat aagagaagtt aaagatatag aaaaattgga
    49681 aaatatttca tttctgggaa agacatcaag aaaaggaaca gggggaatga aaagtaaaat
    49741 acaggcagca aatattgtta caaaaaatgg aaagccctgt ttaataataa atgggaaaaa
    49801 aatgtggaca ctaaaaaaag tttttgaggg aaaagaaata ggaacatttt tttatccagc
    49861 gaggtagaaa aaatgaactg gaaagatgat ataaaaataa aattagaaaa aacaaaaaat
    49921 gcatataagt ccattactgg acttacaaca gaagaaaaaa ataaattttt agaaaattta
    49981 gcacaaaata taaaaaaaga aagtccaaat attttaaaaa gtaataaaaa agatgttgaa
    50041 aaagcacaga tgtctggata ttcaaatgcc ttcattgata ggttatcttt atctgaaaat
    50101 aatatagaaa aaattataag cagcatagaa aatgttagaa atttaccaga ccctgttgga
    50161 aaaattatat gggaaaataa gagaccaaat ggacttatta taaaaagaaa aagagttcca
    50221 attggtgtta ttggcataat ctatgaatca agacctgatg ttacagttga aagtagtatt
    50281 ttatgcataa aagcaggaaa ctgtgtaatt ttaaaagggg ggaaagaagc aaaaaattca
    50341 aataaaaaac ttgttgaaat tataaaaaaa tcactaaaag aagcatcatt accagaagat
    50401 tgtgtaaatt taatttataa gggaggaaga aaaagtgtaa agcatctttt aaagttccat
    50461 cagtatatag accttattat tccacgagga ggggaagaac taataaaaac agtagttgaa
    50521 aatagttcaa tccctgtaat aaaacattat aaaggtatct gtcatactta tattgataaa
    50581 tatacaaata ttgatatggc tttaaaagtt acatttaatg caaaagttca aagaccagga
    50641 acatgtaatg caactgaaac attacttgtg cataaagata ttgcaaaaga atttttacca
    50701 gaaatggcaa aaatgtttga agaagcagga gttgaaatga ggggctgtga agaaacaaga
    50761 aaaattttac catttatcaa aaaagcaagt gaagatgatt ggaaaataga atatttagat
    50821 aaaatcattt caattaaagt tgtaaactca attgaagaag caatagagca tattggaaaa
    50881 tatggaacat ctcattcaga tgcaataata actgaaaata aggaaatggc tgaaaaattt
    50941 ttaaatatgg ttgactctgc tgctgtttac cataatgctt caacaagatt tacagatgga
    51001 ggagaatttg gtttaggagc agaaattgga ataagtactg ataaaataca tgctcgtggt
    51061 cccatgggac ttgaagaact tacaatttac aaatatattg tatatgggaa tggacagatt
    51121 agagaataaa acaattggtc ttttaggtgg aacatttgac ccaattcata ttggacattt
    51181 aataattgct gaaaaagtca gagaagaatt taatttagaa aaggttatat ttattccagc
    51241 aggaattcca cctcataaaa aaagcgcctt cacttcttct tttcatagat acaatatggt
    51301 aaaaattgca gtttctgata attctttttt tgatgtttct gatattgaaa ttaaaagaaa
    51361 tttaccttct tatacatatc taactataaa aaagatgaaa gaaatatata aaaattacaa
    51421 aatttttttt ataattgggc aggactcgct tttagactta ccaaattggt ataaagcaga
    51481 taaattaata aaagaaacta cctttattat tgtaagaaga aatacagatt tttattatga
    51541 aaaagataaa aatttccctg tgaattttga attaagtaat aatccaatta ttggaatttc
    51601 ttcaacttat ataagaactt gcataaaaga aagaaaatca ataaaatatt tattacctga
    51661 aaaagtaaaa aattatattg aaaaggaaaa tatttatgga taaagaagaa ataatcaaaa
    51721 aatgtagaga tgtttttaac cgtggaaaag aggagttaga aaaattaatt gatgaaaatg
    51781 attttgaaaa atggagaaca aagttttttg gtagaaaagg agagattaat aaactttttt
    51841 cttttctttc tactatcaaa aatgaattaa aacccgaaat aggcgaagaa ataaacaaaa
    51901 taaaaataaa tctgatgtct ctttttgaag aaaaaaaaga aataattcaa aaagaaagaa
    51961 ataaagaaaa atttaatctt tcttttcctg ggaaaattcc tgaatatgga ggaattcatc
    52021 cattaactca aattatgaaa aaaatgacaa aaatttttga aaacatggga tttggtattt
    52081 ttacaggacc agaaattgag acggactttt ataattttga ggcattaaat acacctgaag
    52141 accatccagc aagagatata tgggatactt tttatcttga ctataagaaa aagatacttt
    52201 taagaactca tacaagtcct gttcaaataa gagttatgga aactaataaa ccacctttta
    52261 gaataatagc acttggtaaa tgttttagga gagaccaatt cgattcctct cacagtcctg
    52321 cttttcatca aatggaagga cttatggtag ataaaaatat aagttttact catcttaaag
    52381 gtatcttaaa ttattttctt aaagaaatgt ttggcgaaaa cataaaagtc aaatttactc
    52441 cttcatattt tccatttaca gaaccaagtg cagaagtaag tatctcatgt gtaatttgtg
    52501 gaggaaaagg gtgttcaact tgcaaaaaca gtggttttct tgaaatactg ggatgtggaa
    52561 tggttcatcc tcaagttttt agaaatgttg gatataaccc agaagaaata actggttttg
    52621 cttttggaat gggagttgaa agaattgcaa tgcttaaata caaaattgat gatataaggt
    52681 tattttacaa caatgattta agatttttaa aacaatttta aaatgagata ctcatttaaa
    52741 cttttatcag aatacattga tggagagatt aaatttaaac aattaattga atttattgat
    52801 attcttggtt taaatccata tataattgaa aaaaataatg atataatttt tgaacttgaa
    52861 atacctgcta atagaggtga tttgttttct ctaataggaa ttataaaaga aattatccct
    52921 tttactgaaa ataaactact cttaccagaa ataaaatttt cagaagaaat tgacgatata
    52981 aaaagaattg aaatagaaaa tataaatgat tgttcgtatt attcatcaag aataataaaa
    53041 aatttagaaa acatttcatc tccatcagaa tttattgaaa aaattaaaaa attgggattt
    53101 aaaagttctt tcctccctgt tgatatttcc aattttgtta tggctgaaac aggacagcca
    53161 attcatattt ttgatttaga tacattagag ggaaatataa ttataagaag aggaaaagaa
    53221 aatgagaaaa ttacctgtct tgatggaaat gagtataaaa tcaatcctga catacttgtt
    53281 atttctgata gtaaaaaagc agttgcaatt gcaggaatta tgggaggaat aaattcctgt
    53341 gtaacagaaa aaacaaaaaa tattctaatt gaaagtgcta ttttcaatcc aattgttata
    53401 agaaggggaa gtaaaaaatt aggacttatt acagaagcat cagcaagatt tgaaaaagga
    53461 ataaatgtgg aaattacaaa attcgggatg gaaagaactg catacctcat aaaaaaaatg
    53521 tgtgggggaa aagttggaaa agtgaactat tctgggaaaa aagaaagttc tgaaattgaa
    53581 attaaattaa ggaaggaaag agttgataaa attattggag tagaagttga agagaaattt
    53641 attgaagaac attttaaaaa ggtaaatttt gactatacta aacagaataa tatctttaca
    53701 gtaaaaattc caccgtatag gaaagacata aaagaagaaa tagacctaat tgaagaaatc
    53761 gcaaaatata aggggtattc ttctataaaa gaagaaaccc cttatacaaa tattactcca
    53821 accacttcgg atgaaacata tcaaaaatat gagacaatta aagatattat tgtaaaattt
    53881 ggatttaatg aaataataac attaagtata atttcagatg attttaaaaa tatattcggg
    53941 gaagaatatg taagaataga aaatcctctt tcttcaaatt tcgcttttct cagaaactat
    54001 ctttttcccc agatgttaga tacatttaag tataatataa atcatcagat aaaacattta
    54061 aaactttttg aattgggtaa agtttatttt aaagaaaatg aaaaatttaa agaagaaaca
    54121 cacatttcta ttggaatttt aaattcaggt gattactatg atatgaaagg tgttgttgaa
    54181 gaatttttaa gatattccaa tatagaaaat ttaaattaca aattagataa aaattttata
    54241 tctgaggaaa atggattaca atcaattttt tataaagaga aaaaaatagg ggaaattttt
    54301 attccaaaga gagaaatact tgaaaaattt gagattgaac aagaacaaat ttatggtgct
    54361 gaaatatata tagaaaaaat tataaatgaa ataacctttg aaaagaaatt taattattta
    54421 ccaaaatttc cgttttcaaa aagggatttt tcctttattt taccttttga aattgattgg
    54481 ggcaaaatag aaaaagttat tttattagaa aatataccca ttgaaaaaat aaatgttttt
    54541 gatatttata aaggggaaaa tgttcctgct ggaaaaataa gtataagttt ttctatctat
    54601 ttccgctctg atgagaaaac acttatagcc gaagaaattg atgagttttc aaaaaaaatt
    54661 atttcaataa tagaagaaaa atttgcaggg gagttaagag gtgaaaaaaa ataaaataag
    54721 aattctcgga aaagaatatt tatttcaatc tgattttgac gaaaaacaat taaaagaatc
    54781 agaaaaaaaa attgaagaaa ggataaaaca ttatgaaagt caatatccca acgcagataa
    54841 aattgacctt ttagttgtat tcatccttga acttttagaa aacatatata taaaagagaa
    54901 acaaatttgc gagagagaaa aaaaatttga gaatttaaag ggaaaattag aaatgatgga
    54961 aaagaatata aaggagaaat taaatatctt gacaaaagaa atacaataaa ttatcataat
    55021 atacatctga aaaaattttg cggggtggag cagtggtagc tcgccgggcc cataacccgg
    55081 aggtcggccg ttcaaatcgg ccccccgcaa ttttggaaac attataagga attgactaaa
    55141 aaacaccaaa taaaaaaatt ctaaaagaca aatacattgt tttgacaaat tttttataaa
    55201 tggtatactg aaattttccc aaaaaaatag ttattttccc actttacaaa aggagaaagt
    55261 gtgttaggaa agggaaggaa atttgcagtt gactttggaa caacaaaaat aagaattgct
    55321 gattttgaaa atagaggtaa taaaatttat tgtaattttt atcaggaaat tccaattcct
    55381 tattattcac ctgaagaaag agaaaatttc ttgaaaaaag aggcaaaagg aattcttgat
    55441 aaaaataaaa taaaaacagt tattgtgtct ttaccgggta gaggattatt attaagacaa
    55501 cttaatatcc caaaagtacc tcctaaaaaa ttgaaagata ttctaaaata tgaagttcag
    55561 caacaaattc cttttccatt agaagtagtt gaatggaaat atcaaatatt aggagaagag
    55621 ggggtaaatc taaacattct tttatctgca ataaaaaaag aacttgtaaa cgaatatata
    55681 ggtaatatta caggatttgg aattgaccct gtttttcttg ataccgacct ttttgcagtt
    55741 tataatgctt ttagatattc acctttctat aatgaggata aatgtactgc aattcttgaa
    55801 attggagaaa catcatcaaa ttttataata ctttacagaa aaatggtact aatgaggtca
    55861 cttaccattt caggaagcac aatcacttct tcaattatgg agacagaggg aatttcatat
    55921 aaagaagcag aaaataaaaa aattgaaatg ggaatgcaac ttcccgcatg tatttcttct
    55981 attgaaagtt taaacactga aattcaaaat tcagttgatt actggcgatt tactcaaaag
    56041 ggacctgagg tatctgaact ttatatatgt ggaaagacct ctcttcttaa tggatttaaa
    56101 gaatcaattc aagaaaaatc taaaattaaa acagaatatt tttctccact ttcaacaatt
    56161 gaaataaatc cgaaatatga aaatttaagg gaaaatgaag ttgaacttgc tgtcttaaca
    56221 ggaattgctt taagaaatgc aggaatttct tttatcaata tagatatgct tcctgaggaa
    56281 attaaaagag tcagagaatt taagctcaat cgtctttata tatacgtatc tataatatta
    56341 gcagggttaa tttcaataac acctgtacta tttctaaatc aggataaagc aatgttaaaa
    56401 ggaattttaa gtgatataga aatttcttta agtcaatatg aaaaatacga aaaagatgtt
    56461 aataaattac aaaatgagat aaaagaaacc aaagaaaaat gtgaaactat tagtggatta
    56521 ataagcaaaa aaggaacatg gctaaaaaac ttaatatata taggtaggtg cttaccaagt
    56581 agtaaaatat atcttacttc attttcacca ggagcagaat ctcaaaccca acagggagaa
    56641 caagcaccaa tttcacctga aggacaacct gttatgccac cgggagggcc atctatgcca
    56701 ccagcaggag caccaatgcc gccaggagaa ccatctatac ctcaatcacc tcaggaacaa
    56761 ataaaaccaa atgaagtgaa catagaaaca caaaatgtat atacattaaa aggtgaagtc
    56821 gttattaccg atataaaaag tgcctttgat aatttcaaat catttgtaaa aaatatatca
    56881 aatttagaat ttatacaaaa agtggatata agttattgcg aagttaattc tgaaagaaat
    56941 gttatagagt ttactctttt attaaatata aaatgaaaaa aatattagga ttactaaaaa
    57001 agtattcgtt gattttttca ctaatcactg ttgttggaat aattggagta ggatattatt
    57061 tttataacaa atcacagaaa gaaattgaaa aaattgaaaa tgaaataagg gaaaaatata
    57121 caaaagcaaa agaatatgaa aaagcaggag aaaatgctcc ttcacctgaa ctaattggaa
    57181 aattaacaaa agaaaaagag aaatatgaag gaaatttcaa atatatggta aacaattttt
    57241 ctacagaaca accaaaagtc ccaacttttg atatttatcc ctcaatagaa tttaaagaat
    57301 atattttatt ttctgaaaac aaattataca aaaaagcaaa aagaatggaa gtaaatcttc
    57361 cttcttcttt aggctttcca aaaaaagggc ttgtttctcc tgaccagata ccaatattta
    57421 ctcttcaatt tgaagttgtt aataacctta taaatttagt cataaattca ggtatcacat
    57481 ctataacaag tattactccc ggaacatcaa gaaaagtatc attttatgaa atgatgccaa
    57541 taaaacttgt tctaacaggg acaagcaatg aaattatcag atgtctaaaa tattttgata
    57601 atccatcttc ttattttctt gttgaaaatt ttttagtcac aaaacttaat gaaaatttat
    57661 tcaaagtaga gataaattta aatgctgtta tgcttttagt tggggaacaa aaattagcgg
    57721 ggaaaaatga aaaagaaaat ccatcaggat aatatcagag agaaaaatag agaaaaactt
    57781 ttgaatttta tcttagaaaa gaagaaaaaa agagataaag aagatatacc tgacgataaa
    57841 aggagaaatg tatctttatt aaaagaacaa aagaaaaaag aaaaagagga aaggtattta
    57901 tctattcagc aaagaagaga taaagaaaaa ttagaaaaaa taaaagaaag agaaaaaaga
    57961 aaagcagaaa aggaagaaga gagaaagaag agattgctga ttaaaaaaca aaaattagaa
    58021 gaaaaacaga aaaaaagaga agagatagag aaacaaaaac agattataca acaaaagaaa
    58081 gaagaaagag aaaagaaact gcaggaaaaa gaaattgcaa aacaaaaaaa attagaggaa
    58141 agaaaaaagc aacaacagga aatagaacaa aagaaacaac aactactaga agaaaaacag
    58201 aaaaaaagag aagagataga gaaacaaaaa cagattatac annnnnnnnn nnnnnnnnnn
    58261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    58321 nnnnnnnnnn nnnnnnnnnn ncaaaacaaa aaaagttaga ggaaagggaa aaacaacaaa
    58381 aggaaataga acaaaaacaa caattaaaaa aacagacact tctatcttta ataaataaaa
    58441 taaaaagtga aaatgaaaga aaatctcttg ttatcaaagc agaaaaggta gaaaacagaa
    58501 agaagaaaat attacttaaa aaacaaaaat tagaagaaaa acagaaaaaa agagaagaga
    58561 tagagaaaca aaaacagatt atacaacaaa agaaagaaga aagagaaaag aaactgcagg
    58621 aaaaagaaat tgcaaaacaa aaaaagttag aggaaaggga aaaacaacaa aaggaaatag
    58681 aacaaaagaa acaacaatta caattactta aacaacagag agaacaacaa ataaaaacac
    58741 agagagaaga aaaagaaaaa ataaaacagg aaaaggaaga agaaagaaga aaaaaaatag
    58801 aaattgaaaa gagaaaaaac gaaagaagaa aaatcttaat tgcccttact cctattataa
    58861 ataaagcaac tgcaattgat aaagaaacca aaaaacaatt aaatctgata cagcagaaaa
    58921 tatcaaaact tacaaaaaag gttgaaattc aaaagaaaaa aataccggag aaaagtaaaa
    58981 ttgtacctgt tgaaaaaggg aaaattgtaa aaaagacagt taaaaaagaa attcaacctg
    59041 ttatttataa agagccattt aatttggggg atttccttag aaaaaatgct tttaaattta
    59101 ttttcttact tcttcttatt gcatggttta ttgagttgtt tttatttact tctcgtctaa
    59161 aatcaccaga gtcacgttta gaaatgattg taggaaaaac aattgaagaa aaaagagagc
    59221 aaatccaaaa gaagagccct gaaacaatat caaccgaaaa ttttgtagtg aaaaaagagg
    59281 aaattaatat tgaaggagaa agagaccctt tttctcctgg aaaacttaca atggcagtta
    59341 tgaaaaaact tcagccaaca aatatagtac ttacaaaaga accagaagtt ataacaattg
    59401 tgaaaaaacc aaaagttgta agtatattaa aagaaccgac tttaatgcag tcagaacagg
    59461 tatcttctat tgtaaaacca actaaacctt cttctgtttc ttcatcaaaa gaagaacttt
    59521 ctactattga gaaaataaca aaaccatcaa tttctcctat tataattcag aaggaaaaat
    59581 gtcctctaac ttatagaggt agaatgcttt ttaatggtgt agaatatttc tttattgaag
    59641 gagaaaaaag aacatataga gtgacaattg gagatatagt tgagggcttt aaaattctaa
    59701 aaaaggaaga ggataaacta tttctttcaa aaaatggtga gatatatgaa attaatgttt
    59761 tttaacttga ctttttttta aaaaaattta aaatgtagga ggaaaaatga aaaaaataat
    59821 taaaatcgga atttgcataa tttttataat tacttcgttt tcatatgcag aagttgataa
    59881 aaaacaatta gaggaaaaat acaggaaggg aatttcattt tattatgaca aagattatgc
    59941 acaggcactt gtaattttta aagaaattca gggagaagac cctacttata gagcatctca
    60001 aattaaaagg tatataacaa gggcacaaac tgaacttgga aaattgggag taaaggaaaa
    60061 gtttgttgct ggggaatcag aaataaaaga agttaaaata aaaaaagaag gagaacttga
    60121 agctctatca gcggcggcac aaaaaacact tcttgacaca taccagtttt tttcagaaga
    60181 aaagaaaaag tataatgtat ctgactttga tatgacaggt cctgaaagta cattgaaatt
    60241 ggcaaaagaa gcatatgata ataaagaata tacagaagca atacgacttg ccaacaaaag
    60301 cagattagaa ttagaaaata tagtacagaa aaaagttgaa gaaaaaaaag ttgtttcatt
    60361 gggagaaata ggagaaacgc ctgtaacatt aaatttaaca aatgcagacc ttgaacagac
    60421 actaaaactt atttatgacc tgactggggc aaatataatt ttaagcaaag gtataagtgg
    60481 aagagttaca ataaatgtta aagacctgcc gttgaaaaaa gtcctgaact taatctgtga
    60541 ggcaaatcaa ttaaaatatt tagaagaaga tggaattatt aaaattatga ctcaagaaga
    60601 atttaataaa agtgctgaag ttataaaaca gcagaataga agagttttta aaatacttta
    60661 tggagatgca agttctattg caaaatcaat cagagataca tataaaatag atagagttat
    60721 tgcagaccca agaacaaatt caatactctt agatatagat gacccgatac aattggcaag
    60781 aattcaggaa acaatttcaa tgcttgatgc tcctatttcg caggtcctaa ttgaggcaaa
    60841 aattattgaa ataaggactt ctactgataa tatgttgtct attgactggc ttgtatcttc
    60901 tcgtttagtg gatgaactta atgccacttt aacaggacca aaatttggag ataatcctgc
    60961 ttttactcct ggggcttcaa gtacactacc aggtggattt tcttttggat taacaaatgc
    61021 agatgttaat actttaatta gtactcttgc aacccatgga gatgtaaaac ttattcaggc
    61081 accaaaaatt acatgtctaa atggaacaaa tgctataata agtgttatac agaattatcc
    61141 atacattata cctgaatatt ctgaaacata taatgagcag ggtactagaa ctggaacaag
    61201 acaaacggtt actgtttatg aagaaactgt tgggacagag tttgaagtta ctccaataat
    61261 tcaaacagac agaagtgtat ttttaaattt aaatatttat gattcacgac ttgttgaaat
    61321 tagaaaatta actgcaattg ctgctggaca aaggtatgaa acagaacaac caattgtttc
    61381 ttcaagagaa acaaatcaaa gtgtaacact ttatgatgga caaacacttg taataggtgg
    61441 attaattcaa agtgcagaag agaaaaacga atcaggaatt ccttttttaa gaagaatccc
    61501 tgtattagga tatttattta aaaagcccaa atataccaat actaagagtg aacttcttat
    61561 attcctgact cctcatgtaa taacaacatt taaagaagta gaagcagtaa caggaccaga
    61621 tattaaaaaa ggtgaaaaag aaattgaacc tggactatta aaacattttt aatgacttga
    61681 caatttctct aaaaataggt ataaatataa cgaaaatggg aggtgataaa atgaaaaata
    61741 aaattagttt actcctgata ttatcttttt taggatttat gattttacca tctttttcac
    61801 aaacatcaag ttcaaaaagt tcttcatcaa gaacaacaag aaaggatttt cgtggagata
    61861 cagatgataa aaatgaagaa agacagggat acataagaga tttcgttgtg gcagttatag
    61921 gaactaagtt aagacatcct gacccgcaaa taagaatgca agcattacaa tcaatcctta
    61981 caggtattct ctcaggagaa ggagaaggcg gaggagatga aaatgaggga ttaaaaagct
    62041 attttagtat tggtactgaa aatagtagtg gagatacggg agattatgta ggagcaggtg
    62101 ctgctgcttt tattccagat atttattctt tattaaaaga ccctgaccct gaaattagag
    62161 atgttgcagg tgttggattg gatgcattat ttggttcaga tgtaacttta ttaagattta
    62221 tggatgaccc tgaccctgtt gtaagaaaat atgcaacaaa aatctatatc aaaaaagcat
    62281 tggtagcaag tcaggatgat gataataaca atgataatga aaataaaaca ggcgaccttg
    62341 cagaactttt agcattaaga actttgcttg ttcgtctaaa atatgaaaca gatgagggag
    62401 tgagaaaaac aatagaagat agtattgaat attacttaac atcagaaaca ggtagagata
    62461 gaggcgatga aagaggatat gtaaggggag ttgattcttc aattataaag tatcttggtg
    62521 actctaatcc tgaaataaga aaaaatgcag caaaaataat tggtgagttg gaatacaatc
    62581 ccagtattct aaatatcctc atggaagcat taaggaaaga aaataactca gaggtaaaag
    62641 aagaaataca agcatcaata gaaaaattga cagctaatct aagtcaagta cagaaaaaag
    62701 aactaagata gaaggtaata aatcataaat aatgaaaaaa atcttccttt tgtttatttt
    62761 tattttaagt tcattttttg tttgtgctga taattttgtc ttaaatttta ataatgtaga
    62821 tattcaggca tttattaaat ttgtaggtga atttgtccaa gaaaattacg ttatagaccc
    62881 aactgtaaaa ggaaatgtaa caatttattc caacaaacca gttccagttg accagataga
    62941 ccagattttt aaaactgtcc tttctcttta tggttttaca gttataaaaa aaggagatat
    63001 ctcatttatt atccctgttt ctgaaggtaa agcaagaact ggaataataa atataggaga
    63061 agtaccagat gataaaatgg gtgattatat aattcagata atcccattag agtattatcc
    63121 tgcacaaaca atttcacaaa ttttaaatcc ttatatttca aaaggaggac aaattacagt
    63181 tgataccaga acaaatagtt tgatagtttc tgattatgga gaaaatgtaa aaaaaatatt
    63241 tgaaatcgtt aaaaaaattg atactcctgc tccaaaaggc aaagaggaat ttagaatata
    63301 tcgacttgaa aatgctgata gtgaagaaat agcaaaaatt ttaacacaaa tattatcaca
    63361 aaaaacaaga gttgtaacaa gggtacaaac agcagaagtt cctattcagc catcagttgt
    63421 atccgcaaaa tcaacaaatt ctttaattgt tcatgcagaa ccagaagatt ttgatgaaat
    63481 tgaaaaaata ataaagtccc ttgatgtttt gacaaatcag gttttaattg aagcactaat
    63541 tgcagaagtt acttatgata aagcacagga tatcggagta gaatgggtag caccagaagc
    63601 atttgaagtt gataaatatg gaatagcagt agaaggaaat gttggaacac ttcaaaatat
    63661 tgaaacagga ggactttcaa taggtattta caaaggaagt tatgggtatc cattaaatat
    63721 tggagcactt atacgccttt acggaaaaga tagtcatttt aatatacttt cagctccaca
    63781 aattatgaca acagataatc aagaagcaac aataaatata agtgagaata ttccttatct
    63841 taaagaaaca agattcgttg aatcaacaag tggaagtaca ggagatacta taaaaagtta
    63901 tgattataaa gatgttggaa ttacattaaa aataactcca caaataagtc aaaataaata
    63961 tgtcaagtta aaaatttctc aggaagtaac aaaacttatt gaaggaggaa caccagaagc
    64021 accagcaaca gcaaaaaggt cagctgatac aactttgatt gttgctaata ataaaacagt
    64081 tgtgcttggt ggactaataa gaaatgatac agaaaattct gttattaaaa caccattttt
    64141 gggagatatt ccattattag gaaagttatt tagaaaagaa tctgaaaaaa gtactaaaac
    64201 aaatctttta atttttataa cacctcgtat aattagttct tttgaagaag cagaagcaat
    64261 aaaacaggaa aaagaaaaaa ttttcccaaa aggacctgat aataaaaatg gaaaaaattg
    64321 aaaatttaga agaaaaaatt gagccagaag ttttaaatct tttcccatat acttttttaa
    64381 agaaaaattg ttttttacct ttaaatggag aaaatggaga aattattatt gttacagata
    64441 accttttaaa aattgatata gaagaaataa aaaatattgt aaaaaaggaa atatcagtag
    64501 ttattatgcc tactgaaaaa atagaagaat gtattgaaaa attttattat catccaacat
    64561 cttcaaaaag agttatagat gatttaaaag aagaagagga cataacagag ttaaaagatg
    64621 aagatatatc cgggacaaca aatttacttg accttgcaaa taaggcacct gtaataagaa
    64681 tagtaaatca aataatatat agagcaattt caacaagagc aagtgatata catattcagg
    64741 caacagaatc agaagtaaaa ataagataca gaattgatgg tgttcttcat gatatttttg
    64801 ttttgccaaa aaaatttcat cctgctatta tctcaagaat aaaaataatt tcaaatcttg
    64861 atatagcaga gaaaagaatc ccacaggatg gaagaacatc aataaaagtt gatagtaaaa
    64921 aaattgatat aagaatttca ataataccga ctttttttgg agaatcagca gttcttcgtc
    64981 tacttgatag gaatagttat ctttttactc ttgaacaatt gggattttca gaagaaaatt
    65041 ataaaaaatt taaccgttta atttattgtg accatggaat tattttatta acaggcccaa
    65101 caggcagtgg aaaaacaaca acattatatg gagcactttc aaaaataaat aatcctggaa
    65161 ttaacataat taccttggaa gaccctgtag aatacaattt agaaggaata aaccagatac
    65221 atgtgaaccc aaaggttgga cttacatttg ctaatgggct acgagcaatt ttaagacatg
    65281 acccaaatat tataatggtc ggagagataa gagatgttga aacagccgaa atggcaattc
    65341 aagcatcttt aacaggacac ttagttttca gtactcttca tacaaatgat tctgcaagtg
    65401 ccattgtccg gcttattgat atgggaatag aaccatattt aattgcatct tctttaatag
    65461 gagttatggc tcaacgactt gttagagtta attgtccaca ttgcagtcaa tcaattactc
    65521 cctctttaga actcctgaaa gaaatttctt taggagaaca agacttaaaa gagggtaatt
    65581 ttaaagaagg aaagggatgt aaaaaatgtt ataatgaagg atattatggt agaactgcaa
    65641 tatttgaact tcttgcaatt gatgatacca taagaaattt tatacttgaa agagattctt
    65701 cacagaaaat aaaaaattat tcaattaaaa agggaatgaa aacattgaga atggatggag
    65761 cagaaaaagt aaaacaggga ataacaacta tctcagaagt tctaagagtc acccaggagt
    65821 tctgatggaa aactttcaat acattgcact tgataaaaaa ggtaagacaa ttagtggtaa
    65881 aattgagggt aatgatgttg acgatataaa gagaaactta aaagaaaaag ggctttatgt
    65941 tataaaagta gagaaattaa aggaaaaatt tcctttttta aaaaagataa gtgaagaaga
    66001 actaataata tcaataagag aacttgcaac ttttataaat tcaggacttc agatagatga
    66061 ggcattaaca ggtcttattt ctcaaatgaa agaaggtaat ttaaaaaata tctataagga
    66121 aattcaaaaa aatataagag agggacagag tttatcaatg tctttgaaaa aatatccctt
    66181 tattttttct gatacaatta tttctatggt aaaagcagga gaagaaactg gaacacttga
    66241 tattgtttta actaaaatat ctgaatttct tgaaaaaaac catagattta aaacaaaaat
    66301 aatgagtata atgtcttatc caattttaat gctttctgtt gcatctgttg ttgtaatttt
    66361 tatcctttct tttgtaatac caacaatcac aaaagtattt tctcaaattt ctatggaatt
    66421 accaataatg acatctattc ttataaatat aagttcattt cttaaaaaat ggtggtattt
    66481 tgtttttgct ggaattgctt taactctttg gggaataaat gtttttttaa aaaccagtcg
    66541 tggaattaaa ttttctgata atttaaaact taaaacccct ttttttaaag atatttatat
    66601 aaaaaaagag attgtaaatt tttcaagaac aatagcaact ttaattaaag gaggagttga
    66661 catagtagac ggactggtaa taagtagaaa tgttcttaca agtccttcaa taaaaaatga
    66721 acttaataac ataattgaac ttgttacaaa aggtggaact ttatcaaatg cttttagtaa
    66781 ttcttcttat ttcccttatc ttgtaactca acttgtaaat gcaggggaga aaagtggaaa
    66841 tttatcagaa atgtttgata aaattgctga tatatatgaa gaagaaataa atcaaaaatc
    66901 aactaaattt gtaaatttac ttgaaccagg tatgattgtt tttatgggaa ttattgttgg
    66961 ttttattgtg cttgcagttt tactcccaat ttttcagata agtcaatcaa taaaatagtt
    67021 aatcagttaa cgattgaaaa ataacaagta gcaagataaa aataaaatcc ccccattccc
    67081 ccctttttaa aggggggcag agaggggatt tctctttact ctatacttgt tgctactcac
    67141 tacttaaata taaacgggat tgagtaaata tttacggtag gataaaatga ataacattag
    67201 aaagagcgga tttacattta ttgaacttat ggtagtagta gtaattctcg gaattcttgc
    67261 tatgagtgtt atgccaagat tttttgaaag aattgatgaa gcaaaaataa cagcagcaaa
    67321 agttcaaatt caaaattttg aaagtggatt aaaacttttt tatttagata atagtttcta
    67381 tcctggaact gaacagggtt tagaggcatt gattgaaaaa cccacaacag gaaaagttcc
    67441 acaacaatgg agagaaggtg ggtatttaga aaagaaagtt ataccaaaag acccatgggg
    67501 caatccttat gtttatttaa gtcctgggag aaatggtgag gactatgaaa ttatttctta
    67561 tggaagagac ggaaaagaag gaggagaggg accagatgca gatatttcaa gttcgcaact
    67621 ttaaaaatgg atttacagtt attgaattgt ttgttgttat tttaataata tccttttttt
    67681 ttcaataatt tttcctgtaa ttaaaaaatt cacaattcca aaatatccag aaggagtgga
    67741 agaaatttct aactttatat ctaatattaa aaaagaatct ataaaaaatc aacagatact
    67801 ttatttttgt attgatattg atataaatga attctggata gagcaggaaa aaaaagaaaa
    67861 aaataaagaa gaagaaatag gtgaggaaga agagatagaa aaatatcaga catcttttga
    67921 ctttatatta tgcaaaaata ctgatgaaga aaaaggtaat ggaattatga aatttaaaat
    67981 ttttcctgat ggatacgtac aacctggttt tatatacata aatgaccctg aaacaaatat
    68041 aatttggaca atttttataa atccatatct taatattcct gaaatttatg aaggggaggt
    68101 aacatttgaa gaatatcaat aaaaatggtt ttactctact tgaatgtata attgcaatag
    68161 gaatttttgg aattattgca gttgtttgtt ttgaaatgta ttttatggaa ataaaaaaag
    68221 taaaaatatt aagtgaaaaa caagttttat tacaacttgc tcaatcaaaa gcagaacaat
    68281 tactaataga aaatgaagaa ataaaagaag agaacggaga atttgaatca cctttttcag
    68341 attatacatg ggaaattaaa ttatccgata taagttattc agatacagaa aatgattttg
    68401 aaatcactcc atatattttg atagttaaat accaaaataa ttttatttcc tatttcatac
    68461 catttgcaaa agaaaagaaa gaaaatgaaa aataatggtt ttactctaat tgaaatttta
    68521 atttcaacag tcataatttt tattactgtt tctttaattt acctcacata ctataatctt
    68581 gaaaaagaaa ctcttgttat tgaagaaaaa cttgaagcaa ttgaaattaa atttaatttt
    68641 ctcacatcat taaaagaaca acttaaatca gcaatagaaa aaaaagatac cttccagttt
    68701 tcttctgatt caatattatt tgaagcaaca agtaattact caccatatac ttttcaatat
    68761 aaattttata cataccagac agataagggt tttgacctta ttgaagaaag aactaatttg
    68821 ttcacgcaga aaaaaataat aataccaata gcaaaaaatt attattcttt taattttgag
    68881 ttttatgatg gaaacaatta ttttgataat tgggataaag aaaaaatgcc agttggaatc
    68941 agaataaaaa tatcggaagg agaaaatgaa gaattttact attatattaa attacctaca
    69001 aaagatgaaa aaaaagaata aagaaggcat aattcttatt tatgttttgg gatttacgct
    69061 tgcaataact actttacttt tagtcgttca ttataaagtt gagcaattta tggatgaatt
    69121 tacaaacaat acagaattta tccaaatgga aaatttagcg gaagtaggat attctattgg
    69181 gacaaattta cttgaaagtg accaaaattc ttatgattgg tatggagaaa gttggagaaa
    69241 agatatagaa attgatacag aaaaatataa aataaaggta aaaattactg atgaagatgg
    69301 aaaaataaat ataaacaaag taattggaga aaaaggggaa atcaatacat tgcttttaca
    69361 aattctgaaa agtgttttca ctatttctgg atattctgat tcaatggttg attgcctact
    69421 tgattggata gacgaggatg aattaccaag aagttccggg gcagaatcaa cattttataa
    69481 aatgagtgga tatccatatg ttcctcctaa tagaacttta tattcaatag aagaaataac
    69541 tcttataaag gattttaata aagaaataat atatggcaag tcaaaagaac aaaatgaaga
    69601 agataaagaa acaaataatg aagaagaaat gaaaaaagga cttataaatt ttatttcaac
    69661 catttctgat gataaaataa atgtaaatac atgcgaatgg gaaatattaa atagtatggg
    69721 atatactgag gtaaatatag accagataat ggaccaaaga gataggggac cattaagtga
    69781 aaattaccta ttagaaatca atagggaagt gactctgaat aataaaaggg ttattaaata
    69841 taaaagttct aatttttcaa tgaatgtttc agttgaaaat atagaaaata aaaaaataag
    69901 aaactttaaa ttttttgttt ttagagatga aaaaagaggt atgaaactta taagaaaaga
    69961 aaaaatatgg gaaaattaaa tatctatata agagataaca attcaacata ttattacaaa
    70021 aataaagaag aaaaaaggga gtttatagaa gaggtaaata ttaaaaatat tatagaaaaa
    70081 gaaaaaaaca attatattaa ttttattctt tccaaaccag atgttatatt tagaaatttt
    70141 tcttttgaat tttctgatat taaaaagata aaaatggttt taccttctga attagaagaa
    70201 agtttatcag aaaaaattga taatttcctt tttaattata gattttttaa acaagggaaa
    70261 aaaacacttg taaatgtaac aggagtaaaa aaagaatttt ataataattt aatttcttta
    70321 attaaaaata aacaaaaggt atcttttttt actgatgtag aaattctttt ctattctctt
    70381 aaaaatttta taaaagatga aaattatatt gagatttaca ttgataaatt ttatatactt
    70441 ataaatgtag ttgaaaatag agtcatttct tcttctattt cttatatttt tgaaaacaca
    70501 agttcagaaa aaataatttc tgttgtaggt aatacattaa aagaaaaagc agaaaaaatt
    70561 tatataactg gcgaggaaaa agaaatagaa aatttcgcaa acaaaacagg tctaaaaatt
    70621 aataaagtag gtattcttaa acaataccag gaattttatt ttacagaaat aataaactac
    70681 aaaagtattc ccactttaaa ctttagttca tcagaaatta aaataattac tcttactccg
    70741 tttttaatac catttttaat ttttatctta cttttaatta ttcctttaac tttattcaat
    70801 aatttgaaac aaaaacagaa tgaattaaat caaattgaag aaaaaatgat taatgaattc
    70861 agaaaaacat ttcctgatgt taaaaaggta gtggacccag ttatacagac aaaggaaaaa
    70921 ttaaatgaaa caggtaaaac atctttttct ttccaatatt cttcaatatt acaattacta
    70981 atagaagtta caaaaatatt tccagaaaat gtaagaattg aaattgacca attatcttat
    71041 ttaaaaaatg ttctaactat ctctggtttt attgacaatt taaataattt agaaaaagtc
    71101 aaaaaagaaa taaacaaatc atcctatttt gagtcgtatg aaattggcac catatctttt
    71161 gataaaaata acagaataaa ttttagttta accttaaaag taaaaaaatg aataaaaaac
    71221 taataacttt aatagtaatt gctctttttg tttttgaata tcaatttata ataacaccgc
    71281 ttaataaaaa aatcagatca acagcaaatc ttataaataa gaaaaaaaat gattatgaga
    71341 ctctaaaaaa tcttattgaa gaatataatg aaaaaaatag aaataagtca attgaaaatg
    71401 caaaaattgt caataaaaat ttttcgcttt tttcttttgt gggtaaaatt attgaagaaa
    71461 atgctcttgt atcaaatatc aaaagtataa acccattacc tgttgaaaaa aaagacaatt
    71521 ttgagattga aaaaataaaa cttaatattg ataatatacc gcttgaaaaa ctcttaaaat
    71581 ttttagcaaa tatagaagaa tcaaattttc ctgtttacat atcagaattt tctatggtaa
    71641 ggaataaaac taatccttct ctgcttatag tcaatatgga aataattgtt ataaaaaatg
    71701 gctgaaattt atttgataga tgggagtaat attctttata ggtcttatta tgctattgga
    71761 aaacttgaga catctacggg attacaaaca aatgcaattt taggtttctt taatacatta
    71821 tttaaacttc tcaaaaaaca caaaatagag tatataggta tattttttga tatgagaaaa
    71881 ccaaccataa gacatataaa attccatgat tataaaatta aaagaaaacc aatgcctgat
    71941 gaattagaat ttcaaatccc aataataaaa gaaatactca attgtcttgg aatgaaatat
    72001 tttgaaaaag aggggtatga ggcagatgat ataattgcaa cttttacaga gaaatttaaa
    72061 aaagaccaca aaattctaat agttaccggg gacaaagata tactgcaact tattgaagaa
    72121 aatgtaaata taataagtcc tgttaatgaa gaaataaaag atattaatta tatcaaaaaa
    72181 aattttaatt ttgctcctga aaaaattatt gattttctcg ctcttgcagg tgatacttct
    72241 gacaatattc ccggtattcc cggtataggt gagaaaatag cacttaaatt gcttttacaa
    72301 ttttcatctt tagaagatat atacgacaac attgaaaaaa ttgagccaga atctctcagg
    72361 gaaaaaataa taaaaaataa agaaatggct tttctgtcaa aagaacttgt aacacttcat
    72421 aaagatattg atattaaagt caatcttgat gatttaaaag tcatttctcc tgatataaat
    72481 aaattagaag aaatattcac atctcttgaa tttaaaaagt taaaagaaaa actttaccaa
    72541 attttccctg aaataaaaga aaataatcca aaagaaataa tcgctttttc aaatggcata
    72601 acctttgaat ttaataactt acttgaaaat attgaaaatt atagagatat ttttgaaaat
    72661 gaaaacattg aattatatgg ctttgactta aaggagaaat tgaaagttat tcatcgcaaa
    72721 gaaattgatg taaaaaataa attatttgat atttctcttg ctgaatattt aacaggaaaa
    72781 accataaaag aacaggatat ttttaaaaaa gtagaaaaat ataaaaaatt tataaaacag
    72841 atgaaaatgg aaaaattata ttatgagatt gaaattccat taatcaatgt tcttatatgg
    72901 atagaaaaaa atggtattaa agtagataaa gaatattttg agtccttatc aaaacaactt
    72961 gaagaaaaaa ttttaaatct tgaaaatcaa atttataccc aagcaggtga agtttttaat
    73021 ataaattcac cccagcaatt atctaaaatt ctatttgaaa aacttaaact tccagcaaag
    73081 aagaaactta aaacaactta ttcaacagac acaattgtat taaatgagtt agcagccctt
    73141 catccattac caaatttact tattcaatat agagaactct taaaattaaa atcaacatat
    73201 gttgatggac tactttcctt tgtaaaaaac agcaggattt atccaacatt taaccaatta
    73261 tccactgcaa caggaagatt aagttgtagc aatccaaatc tgcaaaatat tcctgtaaga
    73321 actgaaatag gaagcaaaat aagaaaggga tttatttcag aagataattc ttttcttctt
    73381 tcttttgact ataatcaaat agaattgaga attcttgctc atttttcaaa agataactat
    73441 ctcattgagg catttaatca gggaaaagat attcatcaag aaacaggtaa attccttttt
    73501 cctgaaaact atcttttctc acctaacata aataatttat caaaagagca aataaggcga
    73561 atagccaaaa caataaattt tggaataatt tatggtataa gtccttatgg actttctcat
    73621 caacttaata tctctgtgca ggaagcaaaa tttttaatag atagttattt tgaaaaattc
    73681 agaggggtta aaaattatat tgaaaaaaca ataaaagata ttgaggaaaa tggatatgtt
    73741 gaaacatttt ttggaaggag acgatacatt cctgaaataa aaagtccaaa taaaaattta
    73801 caggaattcg cgaaaagagc agcaataaat atgccaattc aaggaacatc tgctgatata
    73861 ataaaacttg caatggtaaa aatctataat aaatttaaaa aaaataattt acagagcaaa
    73921 atagttcttc aaattcatga tgaacttatt ttagaagtta aagaaaatga attagaaata
    73981 atttacagga tagtaaaaga tataatggaa aattgtgtaa ctcttcaggt accaataaaa
    74041 gttgatgtaa aaaaagggaa aaactacctt gaaatggaga caattaatat aactttaaga
    74101 taacaaattt ttaatttgac agaaaaaaag gaaatggttt aattatgttg tattttgtta
    74161 tctttttctg aattttaatt gtaaatataa aataaagcag aaaaaaatta tgaaaaaagt
    74221 aagatatcat ttgcgatttc aagtaatacc agataataaa gtagaagaaa atgcagaaaa
    74281 actcgcagat ttttgtttaa aacataaaat agaagaagtt gtgctttttc ttgcggctga
    74341 agagtggaat aatgggttat tatcaaagaa agaagaagat ttatggtttg atacaattag
    74401 taaagcaaaa aaaactcttg aagataaagg aattactgta agtttaaatc catggatgac
    74461 agttttacat ttagatagag gcagaacttt cccttatgac cgtaaattta aaccaatggt
    74521 atctcctttt ggagaacaaa gtaaagcatg tgcttctttt gcagataaaa actggcaaaa
    74581 atatatacaa aatctttatg gaagatttgc aaaattatac tttagagtta tatggataga
    74641 ggacgacttc agatatcata atcatagccc tcttacttgg ggtggtggat ttgagaagga
    74701 tatatgtaat agattttcta aaaaaatagg cagaaaggca aaaagaaagg agatacttga
    74761 aaaaatttta caacaaggtc caccacatcc ctggcgtagt aaatggttag aggtctggcg
    74821 agaaatacaa cttgaagtag catcaggaat aaaagaagca gtaagaaaaa atactaaagg
    74881 aaaaacaaaa attggtctta tgagttccca cccttctgtt catagtattg agggaagaag
    74941 atggaaatct ctttttgaag ccctttctat agaaaaaaat attgcccata gaccacattt
    75001 tgccttttat tcagaagtac ctggtagaga taaaatttac tctataatga tgcttgatat
    75061 tcaaagaaag tttagacctt ctttttgtga agttgctcca gagatagaga attttccatt
    75121 tactgtttgg agcaagtcag atacctctac atggtcagat atggcttttt ctctattttt
    75181 tggttcggat gcattattac ttgacctttt cccgatgaca ggtaatagca ttattgatga
    75241 agataaagga atagggaaat tacttgatag gagttatgat tcattattat ggattagtaa
    75301 tagattttct aaggaaaata atcttttagg agttggtgta ccatggaaag aagatgcttc
    75361 tgagaaagtt cataccacga agggactctc tatggaggaa ttgatagtag accctttacc
    75421 tgcatggaga actcttctgt catgtggtat tcctgcatct gttgagaaac aaaatgtaaa
    75481 cgcaattttt ggtaattcag cgtggatatt tgatgataaa gaaattttag agatgcttga
    75541 aagcggtcta cttcttgatg gtaattctgc aaaaatactt tgtgaaagag gattcagtaa
    75601 atatatcggg ctggaatata aaaaaactgt tggaagagaa gaatcaaaat attccttaga
    75661 agtaataata aatgaaaata gtggcataaa aaaagggata tatttgagtg taaatcttac
    75721 agaaaaattt aacatttttg aacctgatag gcaaacaatt gtctggtcag aagttattac
    75781 acctgaaaaa gagaaagttg gtgtgggaac tacactctac atgaatgaaa aaggaggaaa
    75841 agtagcagta attgtaccag atattctgaa ttttaatttt ttcagacaaa caataatgca
    75901 aaatctcata aaatatctat acaatatgaa aattcctttt cctatagtat caggcagtcc
    75961 atatcttttt ccaatgtact ttaaaggaga aaaaaaagat tcattggtta ttttcaatgg
    76021 aggaactgat tctgctattg tacaatttga aatagataaa aaaaagaaca cttttggaga
    76081 tataacaatt cttaaaccac tagaaaaacc aactgctgga aatttattaa aaagaggaaa
    76141 tacattctgg aaattatctt caagtattcc atatatgtct ttttttatta tggaattttt
    76201 taaaaataag aattataata atacaaataa ggaggaataa aaaatgaaag tattgaagga
    76261 taaaaatgtg ttggggattt ttgtttgtgg tattattgtt gttttggtct tggtattttg
    76321 cattctaatg acatttaaat caagggttaa tattgcctcg gttgacctat caaaaattat
    76381 aaatgaacac cctgctatgg aagaggcaat gaaaaatttt caaaaagaac ttgataacca
    76441 gcagaaaaaa tatgataagt taaaggatga agaaaaagca aaagaacaac agaaaattca
    76501 gcaagaaatg acacaattag ccatacaaat gcagcaaaat gtagtaaaaa cagcaattgg
    76561 tgatgtggaa agaatagcaa agaaaaatgg ctataactat gtcattgata aaaatgcact
    76621 tattgttggt ggaaaagata tcacagatga agtattaaaa gaattaaaac aaaataaaca
    76681 ggaaaaagta cagaataaag aaaatacatc tgatatgcca gtaattcctg taaatccata
    76741 aaaatatctt cccttgtgag aattttttta tacgagaaat atttttccat ttatgtccat
    76801 tatatccaaa tttgacttat cctacaaagg aaattacaat aaaataatga tagaaagaaa
    76861 aataatagga aaaatgttaa taacatttgg cgttttatta gtcatttctg gattattttt
    76921 ttacaaagga ataaaattcc cacttggaaa attaccaggt gatatattaa taaaaaaaga
    76981 aaattttgtg ttttattttc
//
LOCUS       DAOOVW010000002        69189 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_215_length_69189_cov_7.445266, whole genome shotgun sequence.
ACCESSION   DAOOVW010000002 DAOOVW010000000
VERSION     DAOOVW010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 69189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 69189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69189
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_215_length_69189_cov_7.445266"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(235..1221)
                     /locus_tag="PLW95_00405"
     CDS             complement(235..1221)
                     /locus_tag="PLW95_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21129.1"
                     /translation="MNKIRICVFVILFVNFSIFAEIIIPLTKSKGNDKFAIYSPDGDK
                     IVFIRQENSKEGIWLLDIKEKKEYLLTGQLNSPKDIYFSSDSRRIVFSSEGKIYLLDV
                     SFLSDVKKKYLVNGERPVFSPDGEKIGFIKNRKICVLDNGEEKELTGEYSNLDIAWVN
                     GEEIIFCGDGNIYILNIKDKKVTLISELREKLGNNLYYTKIYVSPNKKEVIAVVPMGL
                     VAPGEDLTRAVGDVLYLVKKDSFEEISYGRNPCWSSDSKKIVFEDEEEIYIYSITEKR
                     KIKLTDDNYLDERPVFSPDGKSIIFSSVRRDTNNDGIINWKDSLDIYKMVIE"
     gene            complement(1248..1457)
                     /locus_tag="PLW95_00410"
     CDS             complement(1248..1457)
                     /locus_tag="PLW95_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21130.1"
                     /translation="MGDLSFAEEKGLVAHWSFDEGSGDIVKDNSSNGNNGKINGAKWV
                     EGVRGYGLEFDGDYVDCGKDVFNRS"
     gene            complement(1515..3830)
                     /locus_tag="PLW95_00415"
     CDS             complement(1515..3830)
                     /locus_tag="PLW95_00415"
                     /inference="COORDINATES: protein motif:HMM:NF039643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6067 family protein"
                     /protein_id="HOV21131.1"
                     /translation="MEKKRVILGLIVSLYFILSIVVDAEVFKFDMGDEKSPVYSGFIK
                     VTKETTYTEEKGYGWTGSSKNLKDGYWKLPDDLAMDYVSGSPAIFKVNIPNGEYKVCY
                     ISPHCNYSNAYYTPGFRYDARWYVKVKGKEVSRLDFNDYMKDVFFKNIDNDYHKNDSI
                     WKKYIRNEFIPRYFIIEVKDNTIEIEFTNGCCITCLVIYPLEKAEQVEKELVEIEKER
                     EKKFNLGIEEIKNEEEKGPFYSISENDRKTGYIIFSRNYLDEIRYNSRPKEGEVKKEL
                     TAFASGDEYEPFTFSLFPLIDLKKVKIEVTELENNKGNKIGKENISLNIVRYIEISKG
                     KFGYFQYPIKYEVFPFYLREYKESDFEEGVSRRFWLTVKVPKDTPEGIYKGKITISPE
                     GNPSSSLEIKLRVLPFNLQPFEDIFIRTAFQDLRYGSSDFDKYMELIKSYLIFIKEYG
                     LTPDTQSLPNRLFGQKIDEKTRCVDLTYYKKYLEYYKEIGFPSKEVVLCLYSPILKNW
                     WGIPGGTKTRQGYYYNQEGREIIKEILLNIKGETNKFGLEPIFLIGGELTNAGIELIR
                     GYIPLYKELKEAGLKLYNNSNGQGEVDLLSPYLYIHGLRDYLMTPENISYIKKVAPDC
                     KYWIYGVETRFKAGFGYCRSGAKGSYTEFLVIPRRDPYNPFDVGWEGLHISFCLPGEE
                     KPVSTLKAERIREGIDDTRYIRMLESLIKEGEKKKIDVTESKKILEEIMDYINPDFSY
                     YYTHSAPSNEVYDKLRWRIANEIIKLNKILK"
     gene            complement(4053..4895)
                     /locus_tag="PLW95_00420"
     CDS             complement(4053..4895)
                     /locus_tag="PLW95_00420"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HOV21132.1"
                     /translation="MKGKKERKFNIFILENNEQMGKESALLAGEILKQFTARLDKKCI
                     ATFAAAPSQDTFLYNLSKNKDIKWENVYAFHLDEYITLPDGHPNTFKEYLKTHIFDKV
                     DIPSKNIFYIKDSGKNADEMIMNYTKLFSDKYKEVKKEDGIYIAFIGIGVNGHIAFNE
                     PETDLWSNELMIKVRIDDISVKQQYDDYKNHPNPKARYKTLEDVPRESLTMSVGAILK
                     ADKILCIVPGKQKANAVKLTIEGEITNKVPASLLRLHKDINLYLDIYSSSLLKDVPEL
                     KMDE"
     gene            complement(5075..5866)
                     /locus_tag="PLW95_00425"
     CDS             complement(5075..5866)
                     /locus_tag="PLW95_00425"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HOV21133.1"
                     /translation="MFDNIVKKNELIKGFEELGLKKGNIVIVHSSLSSFGYVEGGATT
                     VIDSLKEIITENGILAMPSFPAFIGGEYGIAEKEIIFDVRISPTAMGKISDTFWRKEE
                     VKRSLHPTHSVAAWGKEKEWLISGHENCICSCGENTPLHKICKENGKILLIGIGHNSN
                     TTLHTIEDINGAPTRSCLIFYPKVIDYEGQILTVPLHPHFPGLLRKYEKADEICKENK
                     IQNEIKIGNSLCKLIEAGKLFNILSKFVKEDPLFLIDKNYYAELK"
     gene            complement(5911..6537)
                     /gene="rnc"
                     /locus_tag="PLW95_00430"
     CDS             complement(5911..6537)
                     /gene="rnc"
                     /locus_tag="PLW95_00430"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOV21134.1"
                     /translation="MQEIEKIIDYEFKNKKLLEIALTHSSFSTQNSNERFEFIGDLIL
                     NFIVGISIFKKFPDKDEGFYTNLKSGYVNQHYLNSLGEKLEIAKYIKYKGTKPIDLSD
                     FVESIIGAIYLDGGLKKTERFVKKFILSNKIEPLIDYKGELITISRKLSGKLPLYEII
                     DEKGPAHKKNYKVIVKIEGIASKGEGEGKSKKEAEIKAAKDILTKILD"
     gene            complement(6516..7934)
                     /gene="lnt"
                     /locus_tag="PLW95_00435"
     CDS             complement(6516..7934)
                     /gene="lnt"
                     /locus_tag="PLW95_00435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOV21135.1"
                     /translation="MPLIIFLASCISSLIFALSFPPFNFWYFAFFGLTPLIFLSSKYN
                     KLSYFIGFIAGLSFYIFLLNWLFAVSGFFYILLCVYLAIYWGIFFYLSSLYNFNPFVC
                     ASIWFFIEILIENLLTGFPWLSFSLSQSTNHYIWKLPQIIGSKGISFFLILSNFTIFC
                     LFQKKFKVFLISFFVFIISFTLLFFYNPEIKQKGKLKIMIIQPGIKTEKIKESETIIN
                     ILWEMTTKNVKKLKPDVVILPEGSFPDDIFSRPEILKKIKLLCENYKFSLIFGTFRSN
                     NNKYYNSALFLKNGKIEFYDKTHLVPYGEFILGGRWEFIKKIFVNYAGYTPEIERGKE
                     IKNFNYKEIKIAPLICYENIFPEITNKMLESGSNIFVVITNDSWFGNSFGPYQHFYHN
                     VLRALETGRYFIQCGLSGISGIVSEKGEVLKIIDIDKKETLFFEVPLFFQKTIYGKYS
                     DFPLSILSLLIIGGYLCRKLKK"
     gene            complement(7939..8289)
                     /locus_tag="PLW95_00440"
     CDS             complement(7939..8289)
                     /locus_tag="PLW95_00440"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOV21136.1"
                     /translation="MYRCMCKCKIKGGKVTDKNLYYEGSITLDKKIIDKAGLLAGEMV
                     YVLNLNSGARIFTYVIEGKENSGIICLNGPSARFFEKDDEIIVLSISYLDDKELKNYK
                     MSIVELGENNKIKV"
     gene            complement(8318..9172)
                     /gene="panC"
                     /locus_tag="PLW95_00445"
     CDS             complement(8318..9172)
                     /gene="panC"
                     /locus_tag="PLW95_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOV21137.1"
                     /translation="MKNGKMEIFKKKEKIGEKIREIKKTGKKIGFVATMGYLHNGHIS
                     LISKAREENDIVIVSIFVNPTQFGPGEDFERYPRNVNRDIELLEKEKVDIVFIPDADE
                     MYKKDHKAWVNVEEYSEILEGKLRKGHFRGVCTVVAKLFNIIQPDRSYFGWKDAQQLI
                     IIKKMVDDLDIPVEIVGCPTLREKSGLAESSRNIYLTEEEKQKALCLYKALKKIEEMA
                     KKGEKNTKKLIEEGKKIILSTGGVELQYLEIVNIENLQPVEKIDNNTIVLGAIKIGNV
                     HLIDNIFL"
     gene            complement(9144..9611)
                     /gene="folK"
                     /locus_tag="PLW95_00450"
     CDS             complement(9144..9611)
                     /gene="folK"
                     /locus_tag="PLW95_00450"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOV21138.1"
                     /translation="MEKVIIGFGSNKGNREKNIKRAIKLIEKRIYIEKISSFFETKPE
                     EKARGGKFLNGVIEGKTNLKAEELLYFLKSIEKKMGRNFPHKRGDEREIDLDIIFYGN
                     KVIKNNFLQIPHPKWKTRYFVLKPIFEISPHFVDPVEKKSIKEIYEERKNGNF"
     gene            complement(9580..10620)
                     /locus_tag="PLW95_00455"
     CDS             complement(9580..10620)
                     /locus_tag="PLW95_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21139.1"
                     /translation="MKNFIITVSVNSEIINRIFDKRDRIYLDKIENMNKLIEEKHSGK
                     LYLDEENEEFYFYFLTGQENISPAVLRCFYHGIKIEENQTPFLARFFTQYNDIITRID
                     VDLSIEEKRHLLNELKEKFDFNYYIKERDIIIIFDFKFPYFRIPIPEEIEGKNIKKII
                     PDKNDLLPLKNFILITSEVLEKSIINQIRKDLNQSVANLLYLWGYGQLNEIQNLKKRM
                     NCYYMKLENGKYEEIATTLGFNKINNFCSPNENSLYWLTFSPDDKNSSIQLKKLENFD
                     KKVLGEILKEKDIKVLIFFESDEDTQKYFNYVYFTDTKKEKKFLKRKIKNISYLHSLF
                     FNNGKSYHWFWK"
     gene            complement(10626..11381)
                     /locus_tag="PLW95_00460"
     CDS             complement(10626..11381)
                     /locus_tag="PLW95_00460"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOV21140.1"
                     /translation="MLINWGSGYFSGLKLGFIFIILGIEFYFLLFEKWTEREINIIQL
                     IILSFSCFLGFLVGVIKISILSIFFLPFAYIVILGIENFNIRKEENIQEKESIKRYEK
                     AIEKNPQNWAAYLSLGDIYFKKEDFEKATYYYRQCYLLRDEPIIKQKLRTSEREEKIQ
                     KGIIWICPECGEENSGEKNKCKNCGYEKDIIKSVRKDIKEKSEIKKYIVLLIISPIAI
                     GMILLLIKFLPLYFSIFLTIGILYFLLKLFFSW"
     gene            complement(11375..12319)
                     /gene="miaA"
                     /locus_tag="PLW95_00465"
     CDS             complement(11375..12319)
                     /gene="miaA"
                     /locus_tag="PLW95_00465"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOV21141.1"
                     /translation="MLNKNRGKKIIIIAGPTATGKTEIGFKLAKKFNGEIISADSRLI
                     YREITIGTDKPPEWMQKNIPHYFIDIVSLNNDFNVFQFNKEAFFKTEDILKRGKLPII
                     VGGSGLYLRSLTKGLFSIPENTGEKQKEIRDKLNKEETASLYEKLKEIDNKIAIKIHP
                     SDKRRIIRALEIYYLTGKKMSEWQKVKGEYYLEKLGNVYYFILKKEKEEIYKKIEERI
                     ERMLKYGWIEEVRKLLEKGYKKTLIEKGPIGYKEIILHIEGEISFDEMVLMIKKRTKL
                     FVKRQITWFKKEDGILIDAKNEKKVIDEIIEISGLEKC"
     gene            12348..>12838
                     /locus_tag="PLW95_00470"
     CDS             12348..>12838
                     /locus_tag="PLW95_00470"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HOV21142.1"
                     /translation="MEKEKFIEMMERYKQIEIINHFNSLNKTEKKEFLKKIENLNIEK
                     TFSLYEELVKNKIPKDELGEITPPENILKPSENPKFTQYLKVKGHEKLKKGEIAICIV
                     AGGQGTRLGFPYPKGMFPITPIKNKTLFQLFTEKARKISDKYKVDIPLFFMVNPENKV
                     LIE"
     assembly_gap    12839..12938
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12939..13534
                     /locus_tag="PLW95_00475"
     CDS             <12939..13534
                     /locus_tag="PLW95_00475"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:NF013836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HOV21143.1"
                     /translation="ERNAEFSLKVIEKKDPEENVGIFVNKNGKNAVIEYIDFPDELKK
                     KKDKNGNLVFNAGSIGIHYISIDFIEKLNKIGFPLPYHKAIKKINSIYGEVEGIKFET
                     FIFDAIPFAERVSCVLTDREEEFAPLKNKEGVDSPEEVKKAISNQGKKYLRYAGINVP
                     DEILVEISPLFAIDKEEVKEKIQKIKINTKEKFIYLE"
     gene            complement(13560..14426)
                     /locus_tag="PLW95_00480"
     CDS             complement(13560..14426)
                     /locus_tag="PLW95_00480"
                     /inference="COORDINATES: protein motif:HMM:NF012524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOV21144.1"
                     /translation="MVPVIKIEGANISSVWEKSVLAVWEKGIEIKTEYDRNYDQPSKD
                     CTMIMVIKDPFSEPRIHLGFPGGFEDLEKYRQEVVNGIHDHWIDPKENKWTYTYHDRL
                     FNYHLPEENYNKKINQIEYIIEKLSTSNYSRRAQAITWIPFYDVFTYDPPCLQRIWCR
                     ILNIKNKKYLIMSSHWRSRDAYRAAFMNIFGLTCLQKYIADKISEKTGEKVEVGQYTD
                     ISDSYHIYGESFDDFENRFLKTIKERDFYNKNRVKSRTIRSDDPSVIDGFEYGKKLLE
                     IEKQTGQKGKTL"
     gene            complement(14481..15173)
                     /locus_tag="PLW95_00485"
     CDS             complement(14481..15173)
                     /locus_tag="PLW95_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21145.1"
                     /translation="MKREISKGKCFLCGKIYSKKEMTNHLKTCLIKRGFIETPVSQKP
                     CLFHLIIEGHHLYDYWLHILIPFNSTLADLDSFIRNIWVECCGHLSLFRIMDFEFFSD
                     GMDIEPSEEHKELYMDIQVGKILSTGLEFIYLYDFGSTTHLSLKVISSLNYKEKIKKI
                     KLIARNEMPSKTCCICGAKATLVCPYCIDLNKGYFCKKCAEKHECEGGYENFLPVVNS
                     PREGVCGYTGQD"
     gene            15358..17361
                     /gene="ligA"
                     /locus_tag="PLW95_00490"
     CDS             15358..17361
                     /gene="ligA"
                     /locus_tag="PLW95_00490"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOV21146.1"
                     /translation="MEKNVKEKIDELVKLLNYYNYKYYVENNPVVSDYEYDQLYHQLI
                     ELEKKYPQFVRHDSPTQRVGGQPLKEFKTIEHKVPMLSIDNTYSAEELIEFDKRVKKM
                     GEVEEIDYVVELKYDGVAVSLIYENGKFVLGASRGDGYRGDDITENLKTVKTLPLQIP
                     YKKPLEVRGEVYMRKDDFQRLNEEREKSEEPLFANPRNATAGSLKLLDPKEVAKRNLQ
                     LFAYQGILENGYETHWDTMMFLKKITFPVSPYMKHAKNIEEVIDYCNEWQDKRFSLPY
                     NIDGMVVKVNSLKLQKKLGTTTKSPRWVVAYKFPAEQVTTILKDVIVQVGRTGTLTPV
                     AILEPVQISGTTVSRATLHNFDEIKRLGLKIGDRVFVEKGGEIIPNIVKAIPELRDGD
                     EKDIPVPTHCPVCGSPVIKETGEVAIRCPNVRCLAQVKERIIHFASKNAMDIEGLGEK
                     WVNIFVDNGLLSDYGDIYYLKYEDIIKLERMGDRSVKNLLKAIEESKSRPFSRLIFAL
                     GIRHIGTHASEILAENFSSIDELSKGTVDQFCSIPEIGPTMAESIVEFFKNKENLKVI
                     EKLKKAGVKTSREKKEVEKKNILSGLTFVVTGTLKNYTRDEITNYIKELGGNVSSSVS
                     RKTDYIIYGEEPGSKLQKGQELKIKLLTEDEFQELVKNLLTKK"
     gene            17358..18248
                     /gene="xerD"
                     /locus_tag="PLW95_00495"
     CDS             17358..18248
                     /gene="xerD"
                     /locus_tag="PLW95_00495"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOV21147.1"
                     /translation="MIKDIPISKLKDEFLSYLYLEKNLSENTISSYKNDIEKFTTFLR
                     EKKYRLKEIDFSKFTEFLLHLKKKNLSVSSIIRIISSIRNFYKFLYARGIINDYLFIE
                     TPKSERDLPEVLSNEEIEKLLSTTENSKKYKRNIAILELLYGAGIRVSELVNLKIDDI
                     NFNQKFMRIKGKGNRERIAFLNTVALQRINDYLPERQQTKKGNLSPYLFVNNRGNKLS
                     RQFIWKIVKKYSSLANLNKNIKPHTLRHSFATHLLESGLDLRIVQELLGHKNLSTTEI
                     YTHIDRRQIKKIYKKYHPRA"
     gene            18334..18606
                     /locus_tag="PLW95_00500"
     CDS             18334..18606
                     /locus_tag="PLW95_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21148.1"
                     /translation="MAALIGGIVAIIVGILLAGPGPLGQATLWFIGGGIVVFLVLGGI
                     IAIAAGISDIKDKIEEEKEKKKEKEEKKEEEKKEGEIASGSEEKKE"
     gene            18970..19731
                     /locus_tag="PLW95_00505"
     CDS             18970..19731
                     /locus_tag="PLW95_00505"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HOV21149.1"
                     /translation="MSKKEIRENYLENVIGKLKSVEKHQVNNFRKAGEMIADAYQQDK
                     LVHVYGGGGHTCLMVCEMFFRAGGLANINPIMGHDISPFCQALKYLEVERTTGYGRCL
                     IKYYRVQKDDILIIFHNIGANPTTIDAADEAKIIGAKIIAVSSNNWREKLPKNHHIRH
                     PNKKHLFDYADLCIDDENPYGDADYYIEGFNVPIGPTSTIIDAYIAHRIVIETVKSLI
                     SRKIEPPIFRSANLPEGDEYNSKLIERYRNRVKDL"
     gene            19755..21002
                     /locus_tag="PLW95_00510"
     CDS             19755..21002
                     /locus_tag="PLW95_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21150.1"
                     /translation="MQKNIEKNKNSKTPAQKFLDLIRKDIIEIQNDKERLIEMAEEMS
                     DSLLKGGNIFAPPVNKAWTSEFSGRAGGLMCLKGKANHSKDIAFFALPDPRNWEPQKD
                     KVLKTLLKGKAKLFVIGRKEDLGDIKINRQFAGFTGGVRADIGMYRYKNFNPFASTRE
                     FKIFVRGWITTGEMICACIRKGKMPIIYMSVWFEGAIPRNSSFTYTDFSNYYEPSLTK
                     PIVPLEVFHKNRYIPPLPPGYATTEFLQIVKMLIDKIDNQADQISRIGQCLAEAKQKG
                     KKIHVVAAGHCYPGILELPDDNSYPLTWGVSRADLKYAVPSDLKDGDVALHFGYGPTS
                     ISDAKKILKRGVKLIHTSPYGRRIGIPENENFLWFDLPWRPGDAVIDIPGYSVRMLPS
                     SSTAHSIAYNAILCEMAEVMGWQ"
     gene            complement(21053..23497)
                     /gene="leuS"
                     /locus_tag="PLW95_00515"
     CDS             complement(21053..23497)
                     /gene="leuS"
                     /locus_tag="PLW95_00515"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOV21151.1"
                     /translation="MEKYDYENIEEKWQRKWEENNLFKAKFQKDNKKFYILEMFPYTS
                     AQIHMGHVRNYTIGDVVARYKVMNGFNILHPIGYDAFGLPAENAAIKHKIQPWEWTYK
                     NINTIRQQLKKLGISYCWEREVITCSPEYYKWNQYFFIQFFKRNLAYKKQAPANWCPS
                     CQTVLANEQVVDDKCWRCGSLVEQKNFEQWFIKITDYAERLLDFSGIKNWPERVITMQ
                     KNWIGKSTGGEVEFDIPQLGENLTIFTTRPDTLFGCTYLVVSPFHPVVKKLIQKSKNS
                     SEIQNFVERTKKQRIKVIDASKTEKEGIDTEIYGINPVNGEKIPIWLGNYVLMEYGTG
                     AIMAVPAHDERDFEFAKKYNIPIKIVIKNPDWQNLPENLENAYEGEGILVNSGTFDGQ
                     ESEKAKEKIIEFLKNKGKGKKTVNYKLRDWCISRQRYWGTPIPIIYCKKCGTVSVPEE
                     QLPVKLPENIEITGKGESPLKNIPEFVKCKCPICGQESERETDTMDTFVDSSWYFLRY
                     ASEKIEDKPFDKEEVNYWMPVDQYIGGIEHAILHLLYSRFFVKVLKDIGYINFEEPFE
                     NLLCQGMVVKDGSKMSKSKGNVVDPTDIIKKYGVDTLRLFILFAAPPELDLEWSEKGI
                     EGCWRFINRVWRINNEIKNYEGEKNEKNNEVEIAINKTIKGVTEDIENDFHFNTGIAK
                     IMEFLNFFSKIISEKSASKKIIVETWKNFIKIISPFAPHISEELWERNGEKEFIYYQK
                     WPEYKETTEKEDIITVPVQINGKLRGTINVPTVIDEKELFEKIINDKKFSKWVENKEI
                     IKKVYVKGKIFNIVLK"
     gene            23668..24471
                     /locus_tag="PLW95_00520"
     CDS             23668..24471
                     /locus_tag="PLW95_00520"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOV21152.1"
                     /translation="MKIAIQIGLLPGNTLEEKFYKAKEIGFEGIEVYGRDLLNDEKIV
                     EKYKNLSKEMGIQISSICAGYRKFLLDPTHEERQQSRNEIKKLLEFGNYLGVVGLILV
                     PVFGSPKITDLSPYKNAFELEKELLISQLPELVEKAEKEKCALLLEPLNRYETHFMNR
                     IEQGIELCKRIKSEYLKTMADFFHMSIEEVDIARAIKKGGKLISHVHLADSNRILPGM
                     GHTDFKKGFKALKKIGYDKFLALECGVPEPKKENLDRCFHYLKNIIETI"
     gene            24486..25556
                     /locus_tag="PLW95_00525"
     CDS             24486..25556
                     /locus_tag="PLW95_00525"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOV21153.1"
                     /translation="MKKLKVGIIGTGGIAQVSHIPNFQKIEDVEVSAISDINKEKLNY
                     VAEKFNIPKTFTDWEKMLEEDIDAVVICSPNSFHSIQSIKAMEKGKHVLCEKPICLSV
                     EEAEKIFDTVDKTGKVFMGAFPRRFLGETIVLKKMIDNGDFGEIYYLKASYLRRRGIP
                     GLGTWFTNKKLAGGGPMMDVGVHVIDMVLYLANLTDPKIIIGTTFEKFKDKAVDGGWP
                     PIETRKGEKVTNKMDVEDLSCGFVKFSNGATLFVEASWAGNSETGLKASLFGTKNGAQ
                     LPDPENKENPVRIYSEIDGIISDITPQIPILDPYLEEAKHFIECIKENKSPITKKEEI
                     ISVVKIIEGIYKSSETGKAIIY"
     gene            25627..26493
                     /locus_tag="PLW95_00530"
     CDS             25627..26493
                     /locus_tag="PLW95_00530"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOV21154.1"
                     /translation="MKKGIEEKVIKKSTGVPILIREDIEYLDKGHSVFLAYHQEVEIH
                     IIKEGKGFYLIQNKKYIFDKNTAIIIYPDQQHLHNIYPPYSIKKISLYFNPGILKKIN
                     FDINSLPHLIKIPGKNIAKIQLVVDLMKNEISEKKKFCEDTTYSLLNFILNLLKRWKY
                     EKEIPYKQADPLIDKLIEYLNENYYKDIDILHLSKIFYKSESYLSHIFKKFTGIGIKE
                     YIIQKRILESKKILENQNDIKITKVGEKVGFSSFPLFYRAFKKITGISPSSYRDIFLK
                     AENNIKTAENII"
     gene            26536..27570
                     /locus_tag="PLW95_00535"
     CDS             26536..27570
                     /locus_tag="PLW95_00535"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOV21155.1"
                     /translation="MEKKFSWYVSGCHEAYAYLSGLKLDEIYKDYFSCAKAFEVGRKK
                     IKEIFGEQVVVGSPSCAPISYGHIICLGVPVSFPEDSEPGVRPIYKNIDEGIDALKKD
                     VDFSKNELFKHYLGMWEYLKNKFPDEKVYFSGFGWEGPITTAVLLRGTDFYMDIFDCP
                     EKVKNFLSLVTESVIKFVYFTRNINNEPPINSESSGLADDLSSLISPSLWDEFVIPYW
                     ERYYSGLTTGKRTIHVENLKPEHLPYLKKVNISYYDPSVSPALSPKIIKEKIDIPFEW
                     RLPSFQLRDMKKGDVRKWIYEMIKEGADTLFHIIGKECCLGNNPDKVKEFIKTCKEIE
                     KGEDYGKISN"
     gene            27551..28789
                     /locus_tag="PLW95_00540"
     CDS             27551..28789
                     /locus_tag="PLW95_00540"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOV21156.1"
                     /translation="MGKLAIEGGKPVREKPMPGRIMFGEEEKKVVMELIEGGIKGTTE
                     KFLDRYAGENVNKYEEEFAKFFGMKFATAVSSGTAAVHSAIASLDLEPGSEIITSPIT
                     DPGTVMPILFQGLIPIFADVDFDTLNITSETIEKVITKKTKGIIPVHLAGVPCQIDKI
                     MKLGKKYKLKVIEDCAQAHFSIYKNKYVGTFGDIGVFSLMSGKHMTSGGQGGMVITNN
                     EKYYWNAKRFADRGKPFGIKNPKGNISIGLNYRMTELEGAIGRVQLKKIKVVAEKRYK
                     IVEKLRDGFSSFKTFSLWKKPDESKPNYWFCFVCVDDKKLKVDKWKTAEALRAEGMPV
                     GAHYVIPMYENPFIKNRKTFGNSSYPFNPFNPNIKYKCPVAEKTLNSHITLYIHECFT
                     DKEIEDILKAFDKVESAYLK"
     gene            28799..30904
                     /locus_tag="PLW95_00545"
     CDS             28799..30904
                     /locus_tag="PLW95_00545"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HOV21157.1"
                     /translation="MIFHMFRVDYPDNWDKLKELNLEKLAKIKKELYKVDIEWVVGSF
                     GTFPGQGYMVSFNTPYFEKYPGLGDFDYIREYLPYAKKYGIKLVSYLNLHWFSYEFAS
                     KHYDWQQITYDGKIYGEKYPLYGNGTTFCINSPFREWALKMMEEVMKTGIDGVFLDGP
                     GIFPESCYCEYCRKKFKEETGHDLPEWENWSDPIWKEFLLFRKKSFLKFMEDAKKVVK
                     RINKDGIIFINSSHLPEWGIPRYIGELEQYQDFNLSEAFYHLGSVRDYFFYYFVAKYL
                     KVSEKPSSAAMHHAAGIWHWIPLYPQEVKMSISQNLCVKNCLWTAVLNGNDIENPEYW
                     QPIKEVMEKVEDKKEIFDKGKTYAPVGLYVSSSTVYFYISEIKGIYTEEEKVKEENLV
                     LKREKEVKKDKKHKCDDITYKELSGFFEVLVRSHIPFKIINENILEENLKKLDVLILP
                     NTTCLSEKEISLINDFVKKGGKLIMSFESGWYDEYGNKREKNPFIDFEIEDNFPLRNA
                     EQYCIFEVETGKYKKGRLVPRPSYSLKIKTKEKVILSFIKPSKGTYSYCIEKSDYPAI
                     IKKIEGKGKIIYFSNLPGEFYNSFHPLEWENIISEIIKEKIEFEIKVDAPSTTITEFY
                     KDSDNWIIHIVNSTGDMKRPVSEIIPVDVKIEIKGKFELSERVFEDGNLKIEKEKDKT
                     NITLEGISLYEIIYLRRAE"
     gene            30901..31689
                     /locus_tag="PLW95_00550"
     CDS             30901..31689
                     /locus_tag="PLW95_00550"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HOV21158.1"
                     /translation="MKVYIMTDLEGVAGVVDFESQTYNTGKYYEIAKGLLTEEVNSCC
                     DGLVEVGVDEIFVSDGHGSGGILPEKIHREAKLLHGRPLPKFWEIDKKWDCIFLLSHH
                     SMHGTKNGNLNHTYSSRSIVKMELNGEKIGEIGIGVYLAGWFNIPVVFISGDEAACEE
                     AGKYVPNIEKAIVKWGITRTSAISLSPIKAREIIKEKAKKAIEKIDKIKPVKFEGECE
                     LLIEFISTSDAFGFVKRPFFEKVNETTVKVKGKNFLELFERYFY"
     gene            32055..33080
                     /locus_tag="PLW95_00555"
     CDS             32055..33080
                     /locus_tag="PLW95_00555"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HOV21159.1"
                     /translation="MGRYIGVDLHKRSFAVCYRSKAGEIEEKEYSMTEAGIEEFKKRI
                     KKTDEVGVEATRNTGYFIGEIENSVGSIKVINPTQFKIIANSVKKTDKISAKTIAKYL
                     SKGLIPEVRMRSKEQMETLGLIGTRDKLVKLRTSLKNKIHNILNANGIITKKEMFSSD
                     KGLDKILEIDLDEGYLFELRLIVEQLHHLNETIKTIDKELAKRGKKMKGFKNITSMTG
                     ISNTSGTILLSVIGEKNDFERDKKLSAFLWLVPMVYASGDTVRYGMITKMGNKIGRTT
                     VVQSTLVAIKYNEYLRNFYLRLKAKKGSGKAIIATARKYLTIIYKTLRYNLVFEDINH
                     FKLAESS"
     gene            33132..35180
                     /locus_tag="PLW95_00560"
     CDS             33132..35180
                     /locus_tag="PLW95_00560"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HOV21160.1"
                     /translation="MEWWKKEPLRVIEIAHGYEINEKMKVLKELNANMEHLTRYNASE
                     LFPSEEFSLHSGILLKPDDFDKYLKEAHTIGVKVVFYYNVHAVNIQYAKKRPDWQQIC
                     EDGKAIDNVYETYSSFCVNSGWRNEVFEMLKKICKFGFDGIFYDGPIFFANTCYCESC
                     KRLFKGKYGYQLPQKTSLSKDRENSQWKDIIEFQSDAIERFLEDSNNVIKSVNPSVLF
                     YMNCNTLAPTWPTGRNNRKIIRKTDILGAEGGFLYYGLNEPIYKPGATAKFLNSQAYG
                     KPAVVFCAGKQCPWNFSILPKGEITLFYTQTITHGANVWLALCVDEDVRKYKQQVNII
                     KRFNKFIKENPSPFIDNTSSCAKTLLIFPQRSLDFYEGSSVPLTDFTKEIKGEKAGNL
                     IEEFYGFYDSLVRVHIPFDVSDEEGILNIENDKYDLIIFPNAVSLADKEVEKIEGFVK
                     DGGNIISSFETSSHNYGKRLEKFKLGKILGIDDIGKIFGPLRWDSIFIDGKIIFSPEY
                     GIEIKTKGKIISYFAKPLPGSYSKRPEISEIPFIVENKYGKGKSIYFAGTIGSSIYKY
                     HFPEYYKLLSSFYNSLSRKILEIENAPTSLEISIRENEKFYYIYFINFTSEMKRPIEE
                     IMPIRDIKVKFFTNSKIGDVYSLWNKEKLTFKKAKGKIIFNIPIIKEYEIIRVKKEEK
                     "
     gene            35177..36034
                     /locus_tag="PLW95_00565"
     CDS             35177..36034
                     /locus_tag="PLW95_00565"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOV21161.1"
                     /translation="MIDFHTHIGKCFYGRKILTGKKLVKKMDELGIEKSVILPIENPE
                     ETQWYSTTDYILRVSKRYKERLIPFCNVDPRRGMNNGEDPYIGKMIEEYVKKGCNGFG
                     EVLANLEFNDKRMKYIYRICGELKLPVLFHLGGVPGKSTIGLTDKLGLPFMEEVLSEF
                     PETLFIAHGPGWWAEISSDVKEKDRDGYPKGKIEKAGKVCYLLENYPNIYADLSAGSA
                     YNALTRDNDFGIEFLKKHYRKLLFATDYLSVGQELEIVDYMKNVKIPEEIKKMVTKEN
                     AKKILKMEG"
     gene            36036..37355
                     /locus_tag="PLW95_00570"
     CDS             36036..37355
                     /locus_tag="PLW95_00570"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOV21162.1"
                     /translation="MEKLAILGGEKAVKNENEEIFKWPIVNKEMEDKVLDVLRAGKMS
                     ETDITMEFEKEYANWYGMKYALGTNNGTAALHCAFYGIRIGPGDEVICPSVTYWASCI
                     PVLSLGGTVVFADIDPETLSVDPDDIERKISEKTKCIIVVHYCGYPADMDRIMKIAEK
                     YKLKVVEDCSHSHGSLYKGKLTGTFGDVSAFSIMSGKAFAVGEGGIMLTNNREIYERG
                     IVFGHYERHNELTIDYLKDGAGLPWGGYKYRMHQMSSAVGLVQIKKYKKEMEEIDKAM
                     NYFWDLLEGVAGIRALRPLKNSSLTMGGWYSAKGKYYSEELGGLSITKFCEAARAEGV
                     PLSPGCNKPLHTHPVFQNIDIYNEGKPTIISKAARDVRKLYKSLPISEGINKKVFSVP
                     WFKKFVPEIIEQYAYGIKKVVSNYKELLKYDEGDPEEIGGWSLTFHR"
     gene            37798..38622
                     /locus_tag="PLW95_00575"
     CDS             37798..38622
                     /locus_tag="PLW95_00575"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HOV21163.1"
                     /translation="MKIYIMTDLEGVAGVLDFDNWCSPESRYYEVAKEFLTMEVNSAV
                     EGFFEGGIKEIVVADGHGPGGINPKLLDKRVKLLRGWQKWPLLLDKSYDFVAFVGQHA
                     KSRTEFAHIAHTQSFSYFEMRINDIAIGEFGQFSMCASELGVRTIFASGDKALCKEAE
                     ELIPGIVTITVKEGTTPGKGDECPREAYRRRNNAAIHLHSEKARELIKKTAYKAIKKA
                     IEYPDWGIIPLKAPFKRVVIFRPDQDFGKRISIEEHSSSVIEVMNMPYNPKNYDEI"
     gene            38631..39218
                     /locus_tag="PLW95_00580"
     CDS             38631..39218
                     /locus_tag="PLW95_00580"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOV21164.1"
                     /translation="MEYFEKILENALFLLIVINPVSKILIINSLIDEGKQFIKNISVK
                     SSYTGLIILIIFAVGGNFILRNIFRINIYSLQIAGGIILFLFGLNALLKGGFGIDETK
                     DTEEIAMITLASMIAGPGTITATISLSAVYGVISVCISIILAVFINFILMIYSIEIGK
                     LLGKNLLGPLVRLTGFFISSIGMNMAFTGIKEFLK"
     gene            39253..40566
                     /locus_tag="PLW95_00585"
     CDS             39253..40566
                     /locus_tag="PLW95_00585"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOV21165.1"
                     /translation="MEELLCGISKVDITPPVGYYLQGHSGRNKPSKKVHDPLYLKLVC
                     LSDKKTTLYILSSDLIGFSEEFVHNTREELKNKLKISPENFLFTSSHTHTGPFIESTY
                     GGKDKVDPDYLKILQKKIVGAAIESRGNMEKVNVRVGKGKVNIGIVNRRKKTKKGVYM
                     MPNFEGPIDEDVSVIKFEKKDGTSKVILFNYTCHPTTLSTKIYEISADYPGVAQREIE
                     KSYSGCLAMFTNGCCGDVRPALVENGQFIGGNFEDIERMGKILFGEVIKVSEKSISLK
                     EVKLNSKIVNFKFQLEDKLIPKNIKHLEEIVQSYKKQFSYFGPDIIEEWKEDMRGRIE
                     KGEKLNNYIEGYLQFIKIGDIFILGLPGEIMVEIGIKLKEKIGKNLIIFGYSNGLIGY
                     VPTANGIKEGGYEAGSFLYHKYPAPYSEKMESDLINEVINQFKKF"
     gene            40619..41815
                     /locus_tag="PLW95_00590"
     CDS             40619..41815
                     /locus_tag="PLW95_00590"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HOV21166.1"
                     /translation="MERKGIAFLQKKHNKLFGKNKSYLIKVPLRISPLGAHIDHQLGI
                     VSGMTIDKYIFFLFSKNNSKNVKIYSENYNDFVEFSINKNFKKENRWSDYIKGAAHIL
                     KEKFRIKNGINGVVYGEISTGGLSSSAATGISYLLALEKVNEIVVDKTENISLMQKIE
                     NEFIGLNNGILDQSVILLNSLHPQSLIFIDCKSGKYRNVIPKKKFDFQILIVHSGVNR
                     TLLNTNYNERVSECEEAGKILLKFAGKKIKKNIKLRDVKKEYFEKFKDKLPENLKKRA
                     THFYSEIERVKKGVKYWEKGEIEKFGKLMKMSGESSIKNYECGIPETIQLHDILNSIP
                     EIYGARFSGAGFGGSCIALTKGNIEREQIIVNIEKKYIPKFPELKDKYKIFFTNHGRK
                     PEIFEL"
     gene            complement(41835..43118)
                     /locus_tag="PLW95_00595"
     CDS             complement(41835..43118)
                     /locus_tag="PLW95_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HOV21167.1"
                     /translation="MGTGKGEVNRIISEEEIRKILENYLTKENDFSNKKVLVIIPDTT
                     RSGPTALFFHSILDILKKKVKKIDFMIALGTHPEMSDEKIREFLDIKGNEETEIFQHQ
                     WNNSEALEEIGIINKQQMGEYISTLLNENIPVRINRKIFQYDEIIISGPVFPHEVVGF
                     SGGYKYFFPGICGPEFLHKFHWLGALITNPKINGVIKTPVRDVINIAASFINKKVHLI
                     NYVVNENLIYGVFAGDTDDWEKAAQLSSKINIKYVDMPFNMVISVAPPMYDDIWTAGK
                     CMYKLEPVVADGGKLIIFAPHINVVSYTHGKYLEEVGYHTRDYFLKQWEKFKNYPGCI
                     LAHSTHVKGIGTFIDGIEKPRIEVILATGISEEICRKINLGYINPDTLDRENYKNREN
                     EGILVVEKAGEVLYKLKNGKVPDIDQLYKKEVKQS"
     gene            complement(43565..45061)
                     /gene="gndA"
                     /locus_tag="PLW95_00600"
     CDS             complement(43565..45061)
                     /gene="gndA"
                     /locus_tag="PLW95_00600"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR00873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HOV21168.1"
                     /translation="MEKCDIGIIGMEVMGKNLALNFEDKGYTVAVYNRTGEKTKNFVE
                     TQAKGKKIIPAYEIKDFVGLLKKPKKIMMMVKEGKPVDDFIEKLIPFLEKGDIIIDGG
                     NSYFKDTIRRYEYLTEKGILFIGTGVSGGEEGALKGPSIMPGGSKEAYQLIEKMFSDI
                     SAKAYDGSPCVSFIGPGGAGHFVKMTHNGIEYGDMQLIGEVCWFFKNALCMKGEEIGE
                     IMEKWNNKNDILSSYLIEITSDLLKEKDKRNEGVYLVDIVADITRMKGTGTWTVQSAL
                     ELLVPVPTISSAVFSREMSGEKDLRLKMSKILPLNIREELNEKKEDIIDIAHDALYIG
                     KISSYAQGFALLKSASEFYKFNLNLGEIAKIWRAGCIIRAQFLDEITKAYLENPELLN
                     LIAHSKFSDFIKKNLWKLGKLVEISHKVGVPALGLANSYDYILQLTSPIMFACQVTAL
                     QRDYFGAHGYFKIGGEESPEILETENGKIREFHTEWLLPGRPEKEITK"
     gene            complement(45079..46146)
                     /locus_tag="PLW95_00605"
     CDS             complement(45079..46146)
                     /locus_tag="PLW95_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21169.1"
                     /translation="MKKIGIIYQSDEEYIKKIILFFNIECLKGKGNIEFYGIKWDSKE
                     NFEREILKMSNNFIVEKVLLKFSPKFEKVLVLSLIAKEEIKVHKKISAFLKKNNIFQL
                     NPFSSSLKADNKYYTIKKLEKNRINVPKCILLEWEKRGEIEKILKEFLIKNRIENFYI
                     QPNWGTEGRETYFFTSKYFFENKKNVLETIFTILQGQSVIIKEERGNVFFYNEKEKGY
                     RQIVFRVLLWKFNNEIMTDYGFVEIANDNLPITSVEKEGKIIRFKDIFLKVFYREKEE
                     YKRLILTSYDIDLIKKDVLNVYYAFNYKLKELLNFMGIDFLLEFKEGKVIPVFLEINP
                     RPSGINKLEKFDFEGKMSIKN"
     gene            complement(46151..52777)
                     /locus_tag="PLW95_00610"
     CDS             complement(46151..52777)
                     /locus_tag="PLW95_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21170.1"
                     /translation="MKIGKFIKNLKAEGKKEVLLSYISIYEKFSPSQRNKIIKIFSKE
                     KNGQWYINFLEEGEKEKDVIKRKKLCALLLSRKNFGLPYNREKVKYVIETVKDLENLR
                     SQIYEAIRETYDEKQDRECCEKIALDRKESGRRIVCGRFSRAFYIDALLLANSKLLSS
                     NVITLLLEKIRRIISKSKISFLPIESDIQFLDNLTGENDVSIAQIKCGLKNLKDTLRN
                     YEFQLVQKFDENAEFMMQDRKVFDNIRVEIKKFENELNKFPSRTPIYMIFFQRVFPID
                     AIYMGLLNELLDPFFGEDPEIENLLANGGENIYVTPDMKDWLRICDDWIEALPAYATY
                     QIIPENGSYKFTAWVQRNILEEMYRTNAENWALNIEEVMLTENVFLARNILSKILKIN
                     FKDEKQLIERTRNKKKEIAYISALIEKSYENMIRQISEICEREKLLRFEGLTLAINEV
                     EKGNLVKEFLKNYKGIKSLKNQLEKFLEEHCILELHEKYLSLVDYPKRNLPSIHILTT
                     LGPGESELNVKNWLEESMLLFNIIRRHKLEEKVKGKMEIWRQNIIKIGEKIIKENNLE
                     GEIYKLGEGEQGILKCLFSYPEIGKEVSKIIILLQKEGENLNRKDFKVKEPECVLKCL
                     ENINLIDIKEEENLKILKKTREDMIRNFNLEKETKEFLKNYLNPTYSTINAQKEVIVE
                     ENLISELSNPLFRYEAEGPFKRYNLLYTPSRVDLGAKEVFSVRDIPKWVGGIDDISAN
                     SGKELYRLYNIAGPTVVPSTRVTEFLKVGENFFSRGGVYYLSLAGSINLDTLGMGDFE
                     FFRNQWNARGDRKVLPAGETYGGFCVPKEFSLLYAIIIACVDREISSQILSSFGIPEK
                     IQEHIIKDLREVLGWRAEITSELEWEEKATSYLFKKYPEYFGIIGKPICLSRLPQIAK
                     ILGKMGLIENKEKELEYKFTYWVNKKAQGLEEINRTGPFRKVKLIYQLIHQARKKNPY
                     LVDDSELIGVMTANYKEGEKKDGKLIPVSDVRFSAGSRKLEIYSNVADNHILLDIDPE
                     GREIIKEMFKDFIPPADIRIVGSCTGSDILNHVPNSGLEQIKNEVENYLLNIGIDENI
                     IRTNCLVYGGNLKRWIGIKDRTEKEKEKIIKDLEGKIHLLVIDKRGPFSSYEEAIQGI
                     DFIDLGIPDPELLELVDNLPKMLYLMKKGRPNSGLVFADGTSGARRPTFAFNYPNCRR
                     KVKELFALEEKVVYGCLGIGKETIEKWKNQLNDEKSLSEEFLTAILNENKEKAGEVLN
                     RIKKNILIERKFDDVLREERDAKNLGVWNLRDRYITDTFAKISKGIKLNEFDFGKWII
                     YGGSYIINGKLEKEEISELREKYRIKLKKLFGVSGKESYGQKEIDFIVNNFLRSIYHP
                     PKQYKYREISTGLAGSLKAVEEKVEGLARWEERKKEIERVKCIREKKRGFLDVEKVIE
                     NYPKNLSLIYGKAKEIIGKGTNTISPYDFGKYLRISKLYLEFLNKEIVSFNGKNLLQD
                     INNLFSENVITDKNYLSLVTNLASSADVKTGNYEFYNEICAGLELIDISLLIEKTINL
                     NSDEEFNTEISKFFDLTVNNHIFDSLPYHYSKERSSTFEKIPRDKKFQLACQYHRYLY
                     TYLRSLISEKTSMKKFSSDYTDLYLGNFDKNIYGIGIRGENKDEDFWFHYVRLRDSVV
                     LKYEGFGYPEIIMQIDPADLSVSERVNLGIIYPYGNTTVPVALQQGPILSTNANINLF
                     LTSFPIPELKEDKKVLTIREGMFYPNQNDIKKLREKYKKFGENENGLVFGTFKGVITL
                     HGIFFHFTHPLRSEIDTYRIPIIQPLIWEAATHLKCELPKMLKGSGVKTPEQENWYIK
                     DTEKYGGKSKNKIKEKIYKLARKYDALIVKPEKESGGRKSLILPVKENGKYIEENIDK
                     LVELTFEISKTDNVVIQQVLESRVRQLYSKEFLEKLVERFARIGVPVLLDREPQTPLY
                     SYFRQILVFEKSDYKISHHITVISTQGIANVGQGGLLFEYTDDIINPKYRNDMRIQIT
                     KAAFSSMESQRKYLKKNWKYVLNEYLKIYPEFMSKVKYDEIFSDLTGFKINDIPYEMG
                     DYMPVFLIDEDDNLKYIFDEKEEKLIPLYDEKGNPTKIKIFDSNMKEIKRVDKNKKVL
                     SIPLFNEKGEKRKLYDKRGNEIPSLVVYKIEPNPGAGLWRPHNDQLPPERKGEGVFII
                     FKSLGERAKIYKERLESK"
     gene            complement(52779..54275)
                     /locus_tag="PLW95_00615"
     CDS             complement(52779..54275)
                     /locus_tag="PLW95_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HOV21171.1"
                     /translation="MIASKKLNGIENNECLKKLIASYIKKFNDEDIDILKVPARINLL
                     GTHIDHRGGYTNYLTIDKELFCVAGKRNDSKVISYNLESEKYPPREFDIKNELPAKKI
                     NWIDFIRKIKITPGDWINYVKAPILYLQNKFPEIPLKGFNLFYYGEIPIGAGLSSSSA
                     LVVATMLCACKINNIDIEKEKLVEMCGEAEWYVGTRGGSGDHAAMIFGKKGQIVHLRF
                     FPFTSKYLPFPENYKVICCNSLIEAKKSTDAKNIFNERIASYEIGFKLIKKNFPELKN
                     KLVHLRDVNPEILKSEENVYKILLSIPEISKREEICKLLPEENLGVLFETHIPPEKGY
                     RLRDILMYGISECERARICEYFLKNNEIEKFGKLMFISHDGDRVVKHLDNNKIEKWDY
                     RVTDKYLLKLISDLKKGKEEAKIYNQPGGYRCSTPELDFIVDTTKKINGVKGAKLTGA
                     GLGGCVLVLVEEKEAEKTLEILNKKYYHKRNLPESSFICNSSSGAEFI"
     gene            complement(54276..55100)
                     /locus_tag="PLW95_00620"
     CDS             complement(54276..55100)
                     /locus_tag="PLW95_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOV21172.1"
                     /translation="MKFVEKLFSVENKVVVIPGGCGILGGEIAKGFSKSGAKVIICDI
                     KGFEEKREEFKKEGIEVDSMYIDVLKKDEIEKTCKKVIDKYGRIDVLVNAAGGNMKEA
                     TTSDDLSFFDLPLSALEKVLILNLFGGAILPAQVFGKEMVKNKEGGSIINISSMNAFR
                     PLTKIPGYSAAKAAVSNFTQWLAAHFAFEYNKNLRVNAIAPGFFLTQQNRYLLLDENG
                     NLSPRGKTIISHTPMGRMGNPDELIGICIYLSSNASKFVTGIVVPIDGGFNAFSGV"
     gene            complement(55317..56168)
                     /locus_tag="PLW95_00625"
     CDS             complement(55317..56168)
                     /locus_tag="PLW95_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21173.1"
                     /translation="MLKKLNIKLVLMFLIICGLTGSILQAEEIPTIEFIKASLKNQEN
                     LSYPALKVKCQITILTDVEKENSEIISQYVRTPNCFFMETKHFEGKELKFTYKGKYER
                     NSGTRKEISISQSDKKFGEIDMGLGPILSGIKLETTYCPIFLKNSYFLYDALEKGKVT
                     REQKVIDGHKCWLVKISEGDICYNIWLGQDIGFCPVLIERTVNNKIEDTVEFFDYKEV
                     CKDCWFPMKMITEGKTPEGLIKNIWKVEEISVGKAISEEETKIEFLPGTVVSDLTTGT
                     TFEIHKK"
     gene            complement(56189..57106)
                     /locus_tag="PLW95_00630"
     CDS             complement(56189..57106)
                     /locus_tag="PLW95_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21174.1"
                     /translation="MDKIKKIIFLSILVFVLQNACITKTNFVFCEPKDELEFVLQGIR
                     YNLSLIQDASVEFELTYKNSKEIKSGKWFYKDGKEKVFLKAVDESKNINEIIYIYTPE
                     NTYIINFEKGKIASVTMGYSPYYIQLKIRPTWVPSSILFHLWPVGITLEEILQNEKLK
                     ILSNSEYFAGSKCFVVEVPSFGFKEFKGYGKDDYSYLGYKIWFSVEEGFLPKKIEIYR
                     QEDLIISVGPINFEKFSIDKDIWFPGGVSIKWYDPSSKKISAEGKMVYSNIKVNQGIK
                     DEEFNLTFPPGTQVYDERTGISFTVPEKN"
     gene            complement(57519..57731)
                     /locus_tag="PLW95_00635"
     CDS             complement(57519..57731)
                     /locus_tag="PLW95_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21175.1"
                     /translation="MMYKENKSQEQAKKIWEEYLKKYPEGVYNTVVKTFLEGFTEWYH
                     INNYFSCPGHLFKSKCYGCDPGSVKK"
     gene            complement(57772..58152)
                     /locus_tag="PLW95_00640"
     CDS             complement(57772..58152)
                     /locus_tag="PLW95_00640"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOV21176.1"
                     /translation="MKKVEYVNNQKCYPLVYRPSDYKNIDYTFWISEENFLSAKIETK
                     IGSTTTIVSIISYTKIGDIWFPQKFIGYTDIKGEKNAVVEIEYTNIKVNNEISDKIFE
                     PEFQPGMNVYDRITNTIFTVPEKK"
     gene            complement(58139..58675)
                     /locus_tag="PLW95_00645"
     CDS             complement(58139..58675)
                     /locus_tag="PLW95_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21177.1"
                     /translation="MSKIIKIVMWIVPIGYILSNNIGWCQENSKKEFVLKGWEYAVEM
                     IRSGECNYTIKQEQKWYEHPPSVSKSGFFKKEDYEKVPYTKEKKETYNCFWKYKRGKE
                     VVEYIKKTYEKGQGKIYCDGEKYVEIQDKQILITPVGIQNLGIYSVTPPSSVLFHFWP
                     GVSVKKNFFFREDRIEKG"
     gene            complement(58814..59584)
                     /locus_tag="PLW95_00650"
     CDS             complement(58814..59584)
                     /locus_tag="PLW95_00650"
                     /inference="COORDINATES: protein motif:HMM:NF016826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HOV21178.1"
                     /translation="MAQHYKIEKIEGYQQIITPETSDLEFLNCGIIRFSEGEIFKDNT
                     KKNETVFVITEGNCNFFLENKLFGKMKRTNVFDEPPVAVYLPPFLEYRIEFIGKTEIC
                     VLSCLATGKGTPLFIDKKNVKFRRVGEEVYFRNITDILTEESSSERLLLGETINDPGN
                     WSSYPPHKHDKDNPPVETKLEELYFFKIKPKSGFGVMRIFDETEDNIFVVKNDEVITI
                     PKGYHPVAVAPKFQIYYLWVLCGNTKKLISFTHPDFSF"
     gene            complement(59630..61054)
                     /locus_tag="PLW95_00655"
     CDS             complement(59630..61054)
                     /locus_tag="PLW95_00655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOV21179.1"
                     /translation="MKVIITGDSYFIRVVLVENNQIEYFFVDKIPSDIFVGNIYKGKI
                     EKIHKGLNASFINIGKRENGFLHFGDKEVYFGEENFRQLLPETKTHKEGENIIVQVTK
                     PGKELKGMKLTTRITLPGKYLVFIPDSKIKTISKKIKDKKERNRLMTLVKKYIPSNDG
                     FIIRTYAENKDARYIVNEAKYLYNEWKRIKKLDKKFHAPYSLWQELPLHTRIIRDFVN
                     EKTEEIIVEGEKFYNDIKVYIKKWCPEFIRKLKFYNYDIPLFEKFDLNKKILNFLNEK
                     AYLPSGGYLLIEEGSTLTAIDVNTGSFESKSYSETIYNTDIEAAMEIPRQIRVRNLSG
                     LIIIDFIDIKGQEKKKKIFENFKKFLGDEKVKLLSLSSLGLIEMSRKRTGISLKEFFY
                     DECQICKATGYVKNFNMIFIELEENIYNLLFRKGEKRIKVKVHPEFHEFIFKNNLFNS
                     LTQKGRILIEATYQLNGYYSIEIV"
     gene            complement(61032..61574)
                     /locus_tag="PLW95_00660"
     CDS             complement(61032..61574)
                     /locus_tag="PLW95_00660"
                     /inference="COORDINATES: protein motif:HMM:NF021610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HOV21180.1"
                     /translation="MSKDVYRLRVIYEKMGISKYISSKNLLRHIERCLRRTNLPLKYT
                     EGFNPHPKLSFGPPLPVYIEGENEIMDIYFDEKINENEFLKNINQFLVEGVLFKDCFW
                     IPLENHSLGVITLMAVYKIEKDELDLEKLKELGEINKSENFFEIIIKINGFSHKKLLE
                     IVGNQKRIIRNIIYESNYNR"
     gene            complement(61555..63222)
                     /locus_tag="PLW95_00665"
     CDS             complement(61555..63222)
                     /locus_tag="PLW95_00665"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOV21181.1"
                     /translation="MKKFFIFVLLLFSGCSTYQTQKDINQITLSTTSDPKSFNPIIAK
                     ETSTTNITTFIFEGLVEMDGVTLEVKPSLAYKWQIDKSGKKWIFYLRDDVYWNDGEKF
                     SADDVIFTYNDLIYNESIPTSSKDVLTVDGKKINVKKVDDYTVEFQLPEKFAPFLKLM
                     TQEIFPKHILEKNVLNGTITSTWGVNEKVNNIIGTGPYKMKEYRPGEWVILEKNEKYW
                     KKDEKGNRLPYIPKIIFLIIPDPNMTILKFRTGDIDIISIRGQDFSVLEPYQNKENFT
                     IYEIGPSLSSNFLSFNQNVFSPIDNYKIKWFKNLYFRKAIAHSIDKKNIIKNVFNGLA
                     VPQVGPMTSTCGFYYNKNLPDYEYNLSKARQYLLKGGFHYKQTKLFDNENNQIRFTIL
                     TNSDNFERIQIGNVIINDLEKLGMNVNLLPVEFNTLVTKLNVTNDWEGVIIGFTGGIE
                     PHFSKNVWMSYGQLHLWNMGQKKILEEWEKEIDVLFDEGAKELNENKRKVIYDRWQEI
                     VQQQLPLIHTVNPLVIYAVRNKFGNLKPSVYGGVLHNIEEIYVKGCL"
     gene            complement(63219..64085)
                     /locus_tag="PLW95_00670"
     CDS             complement(63219..64085)
                     /locus_tag="PLW95_00670"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOV21182.1"
                     /translation="MRDKNFGGYCLGEVIGGGRYTKVYKAYSFLAHPPYGNPVAIKIL
                     NLKAGIFEKSKLISQFEREAEIAMQLDHPNIVKVFNFGRFNRYYAMIMEYVDGKNLKE
                     ILYEREKFPFETIIKICYKAGKGLSYIHQHNIIHKDIKPDNILVSKNLEIVKITDFGI
                     AKLPGKLWRKDIFPKGGTVTKFGIISYVAPEQEEGNATFQSDIYSFGVTIDEVITSKL
                     QIEGYNDEDYLKRINIRANKKNTGKTPIISYDLPIPDKLKKIIEKAINPDLNLRYSSM
                     DELLADMEEFIK"
     gene            complement(64381..65124)
                     /locus_tag="PLW95_00675"
     CDS             complement(64381..65124)
                     /locus_tag="PLW95_00675"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOV21183.1"
                     /translation="MERVITLPDKGKCVFVGDTHGDIIASKVILKNFLYKNNYYVVFL
                     GDYVDRGKRSKENIDYLLEKREKYDNLILLAGNHEVYPICECSPSNFWEALSPEEFNH
                     YKEIFLKLPWCVTGNNLIGVHAAIPDVEKLLDIENIEVGGKKWFIMLWGDFVEKKGEL
                     LGSFLGRPKLGKDYFEKVMGKLKKNVLIRSHDPKAKEVMFDKRCLTIFTSSEYGVERK
                     IAIVDLEKDIKNVDDIELVSLDLPDLNGI"
     gene            complement(65132..65428)
                     /gene="secG"
                     /locus_tag="PLW95_00680"
     CDS             complement(65132..65428)
                     /gene="secG"
                     /locus_tag="PLW95_00680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOV21184.1"
                     /translation="MYTFLLVFHIIVCLLLMFFVLIQSGKGSSMANIFGGGGMETVFG
                     AETPTILNRITTILAIIFIVSSLILAVTPGKLTNKSILKQQVEKTENKIPPASQ"
     gene            complement(65513..66262)
                     /gene="tpiA"
                     /locus_tag="PLW95_00685"
     CDS             complement(65513..66262)
                     /gene="tpiA"
                     /locus_tag="PLW95_00685"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOV21185.1"
                     /translation="MRKTLIAGNWKMNKTPSESIEFVTNLIKNIGNYNDREILICPPF
                     TSLYPISDIIRNSSLLLGAQNVFYEEKGAFTGEISPLMLKDIGVEYVICGHSERRNIF
                     GEIDEIINKKVKKVIQYKLKPILCIGEKLEERESNKTFDIIKKQLEKCLSDFEYIEQL
                     TIAYEPVWAIGTGKNATPQQAEEVHTFIRKWIGENYNQEIADLIRVIYGGSVSPDNID
                     DLMKEKDIDGVLVGGASLDIDKFLRIINYKV"
     gene            complement(66273..67454)
                     /locus_tag="PLW95_00690"
     CDS             complement(66273..67454)
                     /locus_tag="PLW95_00690"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOV21186.1"
                     /translation="MEIKTIEEQNYSGKKVFLRVDFNVPIENGRITDDTRITASLPTI
                     NYLLSQGAILIISSHLGRPKGKVVPEMSLRPVAERLSELLNKEVKFVPDCVGDEVKKV
                     VENLKKGDVLLLENLRFHKEEEEGNDEFAKQLASLADAYVNDAFAACHREHASIYQIT
                     KYCKDTAAGFLIKKEVDALSKLLSNPEKPFLLIIGGAKVVDKIGMIKNLIDKVDTLIT
                     GGVMAYTFIKAKHWEIGSSKVDEEAIPLAKEIIKEVNKRHLEFHTPLDHIIVNKISPD
                     AEVLITERGTIPQGWIGVDIGPQAIEEYTDCVNRAKTIFWNGPMGIFEIDKFAKGTIE
                     IAKAVANSNAFSVIGGGDSIAAVNKAGVADKISHISTGGGASLEFLEKGTLPGIDVLK
                     K"
     gene            complement(68105..69118)
                     /gene="gap"
                     /locus_tag="PLW95_00695"
     CDS             complement(68105..69118)
                     /gene="gap"
                     /locus_tag="PLW95_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOV21187.1"
                     /translation="MAVKVGINGFGRIGRLVLRSALEKSFPEIDFVAFNDITDAKTLA
                     YLFKYDSNYGIYPGTVEGKDNKLVIDGKEYIVFAEKDPSKLPWKDIGVDIVVESTGLF
                     TDKEKASFHLVGGAKKVVITAPAKGEDLTIVMGVNENMYDPKNHTIISNASCTTNCLA
                     PLVKVLDDNFGVVKGLMTTIHSYTNDQRILDLPHKDLRRARAAALSMIPTTTGAAKAI
                     GKVIPKLNGKMNGLAIRVPTPTVSIVDFVAELEKEVDIQTVNTAFKEAAETYLKGILK
                     YSEDELVSKDFVKNPYSSIYDAPLTYIIGNMVKVLGWYDNEWGYSCRVVDLLKFMVEK
                     GNL"
BASE COUNT        23771 a        10715 c         9451 g        25152 t
ORIGIN      
        1 tccgcaggct cctgtggaaa agttggtctt ccttggtctt tgctatgctc tcaaataact
       61 aactttatac accacttcgg ggttcccagt cacccagtct gattgaatca cctcaaaatt
      121 gtcaaagagc aagtgaacaa gtttaatttt ttaaaagttg cgttctttta aaatttggaa
      181 aacttgtcaa gggatttttg ttgctttttt ctcttgacat tacaccacac atctctattc
      241 aataaccatt ttataaatat ctaaactatc tttccaattt attattccat cattatttgt
      301 atctctccta acagaagaaa aaataatact tttcccatct ggagaaaaaa caggcctttc
      361 atcaaggtaa ttatcatcag ttaattttat cttcctcttt tctgttattg aatatatgta
      421 tatttcctct tcatcctcaa atactatctt tttgctgtca gatgaccaac aagggtttct
      481 tccataactt atctcttcaa aactatcctt tttcacaaga taaaggacat cccctactgc
      541 tcttgttaag tcctctcccg gggcaactaa tcccattgga acaacagcaa taacttcctt
      601 cttatttggt gaaacataaa ttttggtgta gtaaagatta ttacctaatt tttcccttaa
      661 ctcactaata agagtcactt ttttatcttt tatatttagg atatatatat taccatctcc
      721 acaaaaaata atctcctcac cattaaccca tgcaatatcc aaattagaat attcacctgt
      781 taattctttt tcttccccat tatctaaaac acatatcttt ctattcttaa taaatccaat
      841 cttttcccca tctggagaaa aaacaggcct ttcaccatta acaagatatt ttttctttac
      901 atctgacaaa aaactaacat ccaacaaata tattttccct tcactactaa aaacaatcct
      961 cctgctatct gatgaaaaat atatatcctt gggactattc aactgtcctg tcagaagata
     1021 ttcctttttc tcttttatat ctaataacca aatcccttcc tttgaattct cttgcctaat
     1081 aaaaactatt ttatcaccat caggggaata tatagcaaat ttatcatttc ccttggactt
     1141 tgttaaagga attataatct ctgcaaaaat agaaaaatta acaaataaaa tcacaaaaac
     1201 acaaattctt attttattca tcaaaaggtc cttttattct gttgaaacta acttctattg
     1261 aagacatctt taccacaatc aacataatcc ccatcaaatt ctaaaccata acctcttact
     1321 ccctccaccc attttgctcc attaattttc ccgttgtttc cattgctact attatctttt
     1381 actatatcac cagaaccttc atcaaaactc cagtgagcaa caagtccctt ttcttctgca
     1441 aaagataaat ctcccacact cagtaaatac actccggcta aagtaatttt ctttagtatt
     1501 ttcacctcta tttttcattt taaaatttta tttaacttta taatttcgtt agctattctc
     1561 catcttaatt tatcataaac ctcattagat ggagcagaat gtgtataata ataagaaaaa
     1621 tcaggattga tgtaatccat tatttcttct aatatcttct tagattctgt tacatctatt
     1681 tttttcttct cgccttcttt tattaaactt tctaacatcc tgatatatct ggtatcatct
     1741 attccttctc taattctttc tgcttttaga gtggaaactg gtttttcttc accaggaaga
     1801 caaaaagaaa tatgtagacc ttcccaacct acatcaaaag ggttataagg gtcccgacga
     1861 ggtataacaa gaaattcagt ataacttcct tttgctccac tacgacaata accaaaacca
     1921 gccttaaatc ttgtctctac tccatatatc cagtatttac aatctggagc aactttctta
     1981 atataagaaa tattttctgg tgtcatcaag tagtccctta atccatgaat atataaatat
     2041 ggagaaagaa ggtctacctc tccctgtcca ttagaattat tgtataattt caaccctgcc
     2101 tcttttaatt ccttataaag aggaatatat cccctaatta actctattcc tgcattagta
     2161 agttcacctc caatgagaaa aataggctct aaaccaaatt tgttcgtctc tccctttatg
     2221 tttaatagta tctccttaat aatctctctt ccttcctggt tataataata gccctgcctt
     2281 gtttttgtcc caccaggtat tccccaccaa ttcttaagta ttggactata caaacataga
     2341 acaacttctt ttgaaggaaa acctatctct ttatagtatt ctaaatattt tttgtaataa
     2401 gtcaaatcta cacacctggt tttttcatca atcttttgtc cgaaaagtct gtttggcaga
     2461 gattgagtat caggggttaa tccatattct ttaataaaaa taagataact tttaatgagt
     2521 tccatatatt tatcaaaatc gctactacca taccttaaat cttgaaatgc cgtgcgaata
     2581 aaaatatctt caaatggctg aaggttaaat ggtaaaactc ttaacttaat ttctaatgat
     2641 gaagaagggt tgccttctgg agaaatagtt attttcccct tataaatgcc ttctggtgtg
     2701 tcctttggaa cttttactgt aagccaaaat ctcctgctaa ccccttcttc aaaatcactc
     2761 tctttatatt cccttaagta aaatggaaat acttcatact ttataggata ttgaaaataa
     2821 ccaaacttac cttttgagat ttctatatac ctaactatat ttaaactaat attttctttg
     2881 ccaattttat ttcctttatt attttctaat tcagtaacct ctatttttac tttctttaaa
     2941 tcaataagtg gaaaaagtga aaaagtaaat ggttcatact catctccaga agcaaatgca
     3001 gtcagttctt ttttaacctc cccctctttt ggtctgctgt tatatctaat ctcatcaaga
     3061 tagtttctgg aaaaaataat ataacccgtt tttctatcat tctcgctaat ggaataaaaa
     3121 ggcccttttt cttcttcatt ctttatctct tctattccta agttaaactt cttttctctc
     3181 tccttctcta tttctaccag ctctttttct acctgttctg ctttctctaa tggatatata
     3241 actaaacaag taatacaaca accattagta aattctatct ctattgtatt atctttaact
     3301 tcaattataa aatatcttgg aataaattca ttcctgatat acttcttcca gatactgtca
     3361 tttttatgat agtcattatc tatattcttg aaaaaaacat ctttcatata gtcattaaaa
     3421 tcaagtcttg aaacctcttt tccttttact ttaacatacc atctggcatc ataacggaaa
     3481 ccaggtgtat aataagcatt agaataatta caatgcgggc taatataaca aactttatat
     3541 tctccatttg gtatatttac cttaaaaata gcaggtgaac cactaacata gtccattgct
     3601 aaatcatctg gtaatttcca ataaccatct ttcaaattct tggaactacc tgtccatcca
     3661 tatcccttct cttctgtata agttgtctct ttggtaacct taataaatcc agaatagaca
     3721 ggtgacttct catctcccat atcaaactta aatacctctg catcaactac aatacttaaa
     3781 ataaaataca aacttacaat taaccctaaa attactcttt tcttttccat ttcttcctcc
     3841 tcgtttttat ggtgggttct gccaaaattg gctatatctt ttataatgcc tttctttgtc
     3901 ttttaatgct tttatatgtc tttatatccc tgcccctttt tttcatctct ttttttgaca
     3961 ctatcaaatt ttatagtact tgttgttaaa atctcctcta tccctcttta tcaaaggaag
     4021 aattataata aacatctatg gtttaagttg tattattcat ccatttttag ttctggaaca
     4081 tcttttaaaa gtgatgatga ataaatatct aaatataagt taatatcttt atgtaatctt
     4141 aataaagagg caggaacttt atttgtaatt tctccttcaa ttgttagttt tactgcattt
     4201 gctttttgtt ttccaggaac aatacataga attttatctg ctttaagtat tgccccaaca
     4261 ctcattgtta aggactctct tggcacatct tctaatgttt tatatcttgc ctttgggtta
     4321 ggatgatttt tataatcatc atattgttgt tttactgaaa tatcgtctat cctgacttta
     4381 atcattagtt cattactcca taggtctgtc tctggttcat taaaagcaat atgtccattt
     4441 acaccaatac ctatgaaagc aatataaatt ccgtcctctt tttttacttc tttatactta
     4501 tcagaaaata atttcgtata attcataatc atttcatctg catttttccc tgaatcctta
     4561 atgtaaaaaa tgtttttaga aggtatatca actttatcaa aaatatgtgt tttaagatat
     4621 tctttaaaag tgtttggatg tccatctggg agggttatat actcatcaag gtgaaaagca
     4681 tatacatttt cccatttgat atctttattt tttgaaaggt tataaagaaa tgtatcctga
     4741 gaaggagcag cagcaaaagt agcaatacat tttttatcta acctggcagt aaattgtttg
     4801 agaatttctc ctgcaagtaa tgcactttct ttccccattt gctcgttgtt ttctaaaata
     4861 aatatattga attttctttc cttttttcct ttcatttttt gccccctttt taagcaacga
     4921 ttctgccaaa attatctccc caaattcacc ttacactttc tcacaaagac aggtgaaatg
     4981 tgaaaagtaa gatgtgagga tttttatttt tcatctattt ttcatctctt tttgccacta
     5041 cctttatttt atattttaaa tgatttttgt ccaattattt tagttcagca taataatttt
     5101 tatcaatcaa aaataatgga tcttctttaa caaatttact taatatatta aataatttac
     5161 ctgcttctat tagtttacaa agagaattac ctatttttat ttcattttga attttgtttt
     5221 ctttacatat ttcatctgct ttttcatatt tccttaacaa accaggaaaa tgtggatgta
     5281 atggaactgt taaaatttgc ccttcatagt caataacttt tggataaaaa attaaacaac
     5341 ttcttgtagg agcaccgtta atatcttcaa ttgtatgaag agtagtattt gaattatgtc
     5401 caattccaat tagtaagatt ttcccatttt ctttacagat tttatgaaga ggtgtatttt
     5461 caccacatga acatatgcaa ttttcatgtc ccgaaatcaa ccattctttt tccttacccc
     5521 atgcagcaac agaatgagta ggatgtaatg accttttaac ttcttccttt ctccagaagg
     5581 tatcggaaat tttacccata gcagttggtg aaattcttac atcaaaaatt atctcttttt
     5641 ctgcaattcc atattctcca ccaataaaag caggaaaact tggcatggca agaatgccat
     5701 tttctgttat aatttctttc aaactatcta ttacagttgt tgcaccacct tctacatatc
     5761 caaaagaaga aagggaagaa tgaacaataa ctatatttcc tttttttaga cctaattctt
     5821 caaaaccttt tattaattca ttttttttca ctatattatc aaacattttt agcccttttt
     5881 atatttttaa tattttttta aaatgttaat ttaatcaaga attttagtaa gaatgtcttt
     5941 tgctgctttt atttctgcct cttttttgct tttgccttct ccttcacctt tacttgcaat
     6001 accttctatt tttacaataa ctttataatt tttcttatgg gcaggtcctt tttcatcaat
     6061 aatttcatat aacggtaatt ttcctgataa ctttcttgaa attgtaataa gttcaccttt
     6121 ataatcaata agcggttcta ttttattgct taaaataaat tttttaacaa atctttctgt
     6181 tttttttaat ccaccatcta aataaattgc ccctataata ctttcaacaa aatcggataa
     6241 gtcaattggt tttgttcctt tatatttaat atattttgct atctccaatt tttcacccaa
     6301 tgagtttaag taatgttgat taacatatcc tgattttaaa tttgtataaa atccctcgtc
     6361 tttatcgggg aattttttga aaatggaaat accaacaata aaatttaaaa taaggtctcc
     6421 tataaattca aatctttcat ttgaattttg agttgagaaa gaagaatgag ttaaagcaat
     6481 ttctaataat tttttgtttt taaattcata gtcaattatt ttttcaattt cctgcataaa
     6541 tatcctccta taattaaaag agagagaata gaaagcggaa aatcactata tttcccatat
     6601 attgtttttt gaaagaataa tggaacttca aaaaacaatg tttctttttt atcaatatca
     6661 attattttta agacctctcc tttttcagat acaattccac ttataccaga caagccacac
     6721 tgaataaaat atcttcctgt ttctaatgcc cttaaaacat tatggtaaaa gtgctggtag
     6781 gggccaaaag aatttccaaa ccatgagtca tttgtaatta caacaaaaat attactccca
     6841 ctttcaagca ttttatttgt aatttctgga aatatatttt cataacaaat aagaggtgca
     6901 attttaattt ccttataatt aaaattttta atttctttac ctctttcaat ttctggagta
     6961 tatccagcat aatttacaaa tatctttttt ataaattccc atctacctcc taatatgaac
     7021 tctccataag ggacaagatg tgttttatca taaaattcaa ttttaccatt tttcaaaaat
     7081 aaagcagaat tataatattt attattatta cttctgaatg ttccaaatat caaagaaaat
     7141 ttataatttt cacataataa ttttattttt tttaaaattt ctggtcttga aaaaatgtca
     7201 tcaggaaaac ttccttcagg caatataact acatcgggtt ttaatttctt aacatttttt
     7261 gtagtcattt cccaaagaat atttataatt gtttctgatt ctttaatttt ttctgttttt
     7321 atacctggtt gaataatcat aatttttaat tttccttttt gtttaatttc agggttataa
     7381 aagaagagaa gagtgaaaga aataataaat acaaaaaagg aaattaaaaa gaccttaaat
     7441 tttttttgaa ataggcaaaa aattgtaaaa ttagaaagaa ttaaaaaaaa ggaaatacct
     7501 ttacttccga taatttgggg taatttccat atatagtgat ttgttgattg ggaaagagaa
     7561 aaagatagcc atggaaaacc agtaagtaaa ttttcaatta aaatttcaat aaaaaaccat
     7621 atagatgcac aaacaaatgg attaaaatta tataaactgg aaagataaaa aaatataccc
     7681 caataaattg caagatatac acatagcaaa atatagaaaa aaccagagac agcaaataac
     7741 cagttaagta aaaaaatata aaatgaaagt ccagcaataa aaccaataaa ataagataat
     7801 ttattatatt ttgaagaaag aaaaatcaaa ggagtaagtc caaaaaacgc aaaataccaa
     7861 aaattaaatg gtggaaaaga taaggcaaaa ataagagatg atatacaact ggcaagaaaa
     7921 ataataagag gcatagtttt aaacctttat tttgttattt tctccaagtt ctactatact
     7981 cattttataa tttttcagtt ctttgtcatc aagataacta attgaaagga caataatttc
     8041 atcatctttt tcaaaaaatc ttgcagatgg tccatttaaa catattattc ctgaattctc
     8101 tttcccttca attacatatg taaaaattct tgctccacta tttaaattta aaacatagac
     8161 catttctcct gcaagtaagc cagctttatc aattatcttt ttatcaagtg tgatgcttcc
     8221 ttcataatat aaatttttat cggttacttt tccacctttt attttacatt tacacatgca
     8281 tctatacatt attttcatcc tctttattgt atcgttatta taaaaaaata ttatctataa
     8341 gatggacatt tcctattttt attgcaccaa gtactattgt attattatca attttttcaa
     8401 ctggttgtaa attttctata tttacaattt caagatattg taattctact cctcctgttg
     8461 aaagaataat tttctttcct tcttcaatca atttctttgt gtttttttct cccttttttg
     8521 ccatttcttc aattttttta agtgccttat agagacaaag tgctttttgt ttttcttctt
     8581 cggtcagata gatatttctc gaactttctg caagtccact tttttctctt agtgttggac
     8641 atccaacaat ttcaacaggt atatctaaat catcaaccat tttttttata attattagtt
     8701 gttgagcatc tttccacccg aaataacttc tatctggctg aataatgtta aaaagttttg
     8761 ccacaacagt acaaacacct ctgaaatgac cttttcttaa ttttccttca agaatttcag
     8821 aatattcttc tacattaacc catgctttat ggtccttctt atacatctca tcagcatcag
     8881 gaatgaaaac tatatcaact ttctcttttt caagtaattc aatatctcta tttacatttc
     8941 ttggatacct ttcaaagtcc tctcctggac caaattgagt tgggtttaca aatatactta
     9001 caattacaat atcattttct tctcttgctt tacttataag ggaaatatgc ccattatgta
     9061 aatatcccat tgttgcaaca aatccaattt ttttaccagt tttctttatt tctcttattt
     9121 tttcccctat tttttccttc tttttaaaaa tttccatttt tccgttcttc atatatttct
     9181 tttatacttt ttttttcaac agggtcaaca aaatgaggtg atatttcaaa aattggttta
     9241 agaacaaaat atcttgtttt ccatttagga tgagggattt gtaaaaaatt attttttata
     9301 actttatttc cataaaaaat tatatcaagg tcaatttctc tttcatctcc tcttttgtga
     9361 ggaaagtttc tccccatttt tttttctatt gactttaaaa agtataaaag ttcttctgct
     9421 tttaaatttg ttttaccttc aataacccca tttaaaaatt ttcctcctct tgctttttct
     9481 tctggttttg tttcaaaaaa tgaagaaatc ttttcaatat aaatcctttt ctcaatcaat
     9541 tttattgccc tttttatatt tttctctcta tttcctttat tacttccaaa accaatgata
     9601 actttttcca ttattaaaaa ataatgaatg taaataactt atgtttttta ttttcctttt
     9661 aagaaatttt ttttcttttt ttgtgtcagt aaaataaaca tagttaaagt atttttgtgt
     9721 gtcttcatca ctttcaaaaa aaattaggac tttaatatct ttttctttta aaatttctcc
     9781 taacactttt ttgtcaaaat tttcgagttt tttaagttgt atagaagaat ttttatcatc
     9841 aggagaaaat gtaagccaat aaagtgaatt ttcatttggt gaacaaaaat tatttatttt
     9901 attaaaacca agagttgttg caatttcctc atatttacca ttttcaagct tcatataata
     9961 gcaattcatc ctttttttta aattttgtat ttcatttaat tgtccatatc cccataaata
    10021 aagtaaatta gcaaccgact gattcaggtc ttttcttatt tgattgataa tacttttttc
    10081 aagaacttct gatgttatca gaataaaatt ctttaaagga agtaagtcat ttttatcagg
    10141 aattattttt ttaatatttt tcccttctat ttcttcgggt atgggaattc taaaatatgg
    10201 gaatttaaaa tcaaaaatta taataatatc cctttcttta atataataat taaaatcaaa
    10261 tttttctttc aattcattaa gtaaatgtct tttttcttca attgataggt caacatctat
    10321 tcttgtaata atatcattat attgagtaaa aaatctagca agaaagggag tttggttttc
    10381 ctcaattttt ataccatggt agaaacatct taaaactgct ggggaaatat tttcctgtcc
    10441 tgttaaaaag taaaaataaa actcctcatt ttcttcgtct aaatacaact ttcctgaatg
    10501 cttttcttca atcaacttgt tcatattttc aatcttatca agataaattc tatctctttt
    10561 atcaaaaatt ctatttatta tttcactatt aactgataca gttattatga agtttttcat
    10621 ctcaattacc acgaaaaaaa taattttaaa agaaaataaa gaattccaat tgttaaaaat
    10681 atcgaaaaat atagtggcag aaatttaatt aaaagcaaaa tcatccctat agcaattgga
    10741 gaaataataa gtaatactat atattttttt atttcactct tttcctttat atcttttctt
    10801 actgatttta tgatgtcttt ttcataacca caattcttgc atttattctt ttcaccacta
    10861 ttttcttctc cgcattcggg acatatccat attatccctt tttgtatttt ctcttctctt
    10921 tcagatgtcc taagtttttg ttttataatt ggttcatctc ttaaaagata acactgtcgg
    10981 taataataag ttgctttttc aaagtcctct tttttaaaat aaatatcccc caatgaaaga
    11041 tatgcagccc aattttgggg atttttttca attgcttttt catatctttt aatactttcc
    11101 ttttcctgta tattttcttc ttttcttata ttgaagtttt caattcctaa tataacaatg
    11161 tatgcaaatg gaagaaaaaa tattgaaaga attgatatct ttattacacc aaccagaaat
    11221 ccaagaaagc aggaaaaaga aagaattata agttgtatta tattaatttc tctttcagtc
    11281 catttttcaa atagtaaaaa gtaaaattct atacctaaaa ttataaatat aaagcccaat
    11341 tttaatcctg aaaaataacc actaccccag ttaattaaca tttttcaagt cccgaaattt
    11401 ctataatttc atctattact tttttttcat ttttagcatc aatcaaaatt ccatcctctt
    11461 ttttaaacca tgttatttgt ctttttacaa ataattttgt tcttttttta atcattaaaa
    11521 ccatttcatc aaatgaaatt tcaccttcta tatgtaaaat tatttcttta tacccaatag
    11581 gacctttttc aatcaatgtc tttttataac ctttttcaag aagttttctg acttcttcaa
    11641 tccatccata ttttaacatt ctttctatcc tttcttctat ttttttgtat atttcctctt
    11701 tttctttttt taatataaag taataaacat ttccaagttt ttcaaggtaa tattcacctt
    11761 ttactttttg ccactcgctc atttttttcc ctgttaggta atatatttca agtgccctta
    11821 ttattcttct tttatctgat ggatggattt taatcgcaat tttattatca atttctttta
    11881 atttttcata aagagatgct gtttcttcct tatttaattt gtctcttatt tccttttgtt
    11941 tttctcctgt attttcaggt atagaaaaca atccttttgt taatgacctt aaataaagtc
    12001 cgcttccccc aacaattata ggtaattttc ccctctttaa aatatcttca gttttaaaaa
    12061 atgcttcctt attaaattga aaaacattaa aatcattatt tagtgaaaca atatcaataa
    12121 aatagtgagg gatatttttt tgcatccatt caggtggttt atcagttcct attgtaattt
    12181 cccgatagat gagacgacta tccgctgaaa taatttctcc attaaatttt tttgctaatt
    12241 taaaaccaat ttctgtttta cctgttgctg ttggtccagc aattataatt atttttttac
    12301 ccctgttttt gtttagcata taataattat aaattaaggg aggaaaaatg gaaaaagaga
    12361 aatttataga aatgatggaa agatataaac aaattgaaat tataaatcat tttaattcac
    12421 taaataaaac agaaaaaaag gaatttttaa agaaaattga aaatttaaat attgagaaaa
    12481 cattttctct ttatgaagaa cttgtaaaga ataaaatccc aaaagacgaa cttggtgaaa
    12541 taacccctcc tgaaaacata ttaaagccat ctgaaaatcc taaatttacc caatacttaa
    12601 aagtaaaagg gcatgagaaa ttaaaaaaag gtgaaattgc aatatgtata gttgcaggtg
    12661 gacagggaac tcggcttggt tttccatatc caaaaggaat gtttccaata actcccataa
    12721 aaaacaaaac acttttccaa ctttttactg aaaaagcaag aaagatttct gataaatata
    12781 aagttgatat tccacttttt ttcatggtaa atcctgaaaa taaagttcta attgaaaann
    12841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca gagagaaatg cagagttttc
    12961 tcttaaagtg attgaaaaaa aagaccctga agaaaatgta ggaatatttg taaataaaaa
    13021 tggaaaaaat gcagtaatag aatatattga tttccctgat gagttaaaaa agaaaaagga
    13081 taaaaacggc aatttagttt ttaatgcggg aagtattggt attcactata tatctattga
    13141 ttttattgaa aaattaaata aaattggttt tcctcttcca tatcataaag caataaaaaa
    13201 aataaattca atttatggag aagttgaggg aattaaattt gagacattta tttttgatgc
    13261 aattccattt gcagaaagag tttcatgtgt attaacagat agagaagaag aatttgctcc
    13321 tttgaaaaat aaagaaggag ttgattctcc cgaagaagta aaaaaagcaa tatcaaatca
    13381 gggaaaaaaa tatttgagat atgcagggat aaatgtcccg gatgaaatat tagttgaaat
    13441 cagtccgctt tttgcaattg ataaagaaga agtcaaagaa aaaatacaaa aaattaaaat
    13501 aaacacaaaa gaaaaattta tctaccttga ataattgtaa aaagggcaac tttaaaaaat
    13561 taaagggtct tacctttttg tcccgtctgt ttttctattt caagtaattt tttcccatat
    13621 tcaaaaccat caataacaga agggtcatca ctccttatag tcctactttt aactctgttt
    13681 ttattgtaaa aatctctctc tttaatagtt tttaaaaacc tgttttcaaa gtcgtcaaaa
    13741 ctttctccat aaatgtgata tgaatcagaa atatctgtat attgtcctac ttcaactttt
    13801 tcccctgttt tttctgatat tttatctgca atatattttt gtagacatgt taaaccaaaa
    13861 atattcataa aagcagccct atatgcatct cttgaacgcc aatgactgct cataattaga
    13921 tattttttat ttttaatatt taaaattcta caccatattc tttgtaaaca aggtgggtca
    13981 taggtaaaaa catcataaaa aggaatccat gtaattgcct gcgcccttct tgaataatta
    14041 cttgttgaaa gtttttcaat tatatattca atttgattta tttttttatt ataattttct
    14101 tctggtaaat ggtaattaaa aaggcggtca tgatatgtat atgtccactt attttctttt
    14161 gggtctatcc aatggtcatg aattccatta acaacttcct gtctatattt ttcaaggtct
    14221 tcaaaaccac cagggaaacc taaatgaatt cttggttctg aaaatgggtc ttttattacc
    14281 attatcattg tacaatcttt actgggttgg tcataattcc tatcatattc tgttttaatt
    14341 tcaattcctt tttcccaaac agcaagaaca cttttttccc atacagaaga aatatttgct
    14401 ccctcaattt ttataacagg taccatattt tctcccattt tattccttat ctatttaatg
    14461 tctttttaca caataattac ctaatcttgt ccagtatatc cacacactcc ttctcttggt
    14521 gaattaacga ctggtagaaa attttcatat ccaccttcac attcgtgttt ttcagcacat
    14581 ttcttacaaa aatacccttt attaaggtca atacagtagg gacataccaa tgttgctttt
    14641 gctccacaaa tacaacatgt ttttgaaggc atctcatttc ttgctattaa cttaattttt
    14701 ttaatttttt ccttataatt taatgaactt ataactttta aagaaagatg tgtagtagaa
    14761 ccaaaatcat ataaatagat aaattctaat cctgtagata aaattttgcc tacttgaata
    14821 tccatataca actctttatg ttcttctgat ggttctatat ccatcccatc tgaaaaaaat
    14881 tcaaaatcca taattctaaa taaacttaaa tgtccacaac attcaaccca gatatttctt
    14941 ataaaactat caaggtcggc aagagtggaa ttaaacggaa ttaaaatatg tagccaataa
    15001 tcatataaat gatgtccttc aattattaaa tgaaatagac atggtttttg agaaactggc
    15061 gtttcaataa atcccctttt tatcaagcag gttttaagat gatttgtcat ttcttttttg
    15121 ctataaattt taccacataa aaaacatttt ccctttgata tttctctttt catattttcc
    15181 tcttttataa agaattaatt tttcttaata gaatatcagg agaatttaat tttaatattc
    15241 ttgttgtatt ataacatact aaaataaaaa atatcataaa atttttcttt ttgagctttt
    15301 caattaaatt atcgtatgaa tatttattga taatatcaaa aaagatataa tattaaaatg
    15361 gaaaaaaatg taaaagaaaa gattgatgaa cttgtaaaat tgttaaatta ttataactat
    15421 aaatattatg ttgagaataa tccagttgta agtgattatg aatatgacca actttatcat
    15481 caacttatag aacttgagaa aaaatatcct caatttgtaa gacatgactc tccaactcaa
    15541 cgagtaggcg gacaacctct aaaagaattt aaaacaatag aacataaagt tccaatgtta
    15601 agcattgata atacatattc agcagaagaa cttattgaat ttgataaaag agtaaaaaag
    15661 atgggagaag ttgaagaaat tgattatgta gttgaattaa aatatgatgg tgttgctgta
    15721 tctttgattt atgaaaatgg aaaatttgtt ttgggagcaa gtagaggtga tggatataga
    15781 ggtgatgata taacagaaaa tttaaaaaca gttaaaacat tacccttaca aattccttat
    15841 aaaaaaccat tagaagttag gggtgaagtt tatatgagga aagatgattt tcaacgactt
    15901 aatgaagaaa gagaaaaaag tgaagaacca ctttttgcta atccaaggaa tgctactgct
    15961 ggttctttaa aacttcttga cccaaaggaa gttgcaaaaa gaaatttgca attatttgca
    16021 tatcagggta ttttagaaaa tgggtatgaa acgcactggg atacaatgat gtttttaaaa
    16081 aaaattacat tcccagtaag tccttatatg aagcatgcaa aaaatataga agaagtaatt
    16141 gactattgca atgagtggca ggataaaaga ttttcacttc cttataatat tgacggaatg
    16201 gtagtaaaag ttaattcttt aaaattacaa aagaaacttg gcactacaac taaaagccca
    16261 agatgggttg ttgcatataa atttccagca gaacaggtta ctactatatt aaaagatgtt
    16321 attgttcagg ttggtagaac tggcacactc actcctgttg caattttaga gccagttcaa
    16381 ataagtggaa caactgtttc aagagcaact ttacataatt ttgatgaaat aaagagattg
    16441 ggattgaaaa taggggatag agtttttgtt gagaaggggg gagaaataat tccaaatatt
    16501 gttaaagcaa ttcctgaatt aagagatgga gatgaaaaag atatccctgt tcctacacat
    16561 tgccctgtgt gtggaagtcc tgttattaag gaaacaggag aagtagcaat aagatgccca
    16621 aatgttagat gtcttgcaca ggtaaaagag agaataatcc attttgcatc aaaaaatgct
    16681 atggatatag aaggacttgg agaaaaatgg gttaacattt ttgttgataa tggacttctt
    16741 tctgattatg gagatattta ttacttaaaa tatgaagata ttataaaatt agagagaatg
    16801 ggggataggt cagttaaaaa tcttttaaaa gcaatagaag aaagtaaaag cagaccattt
    16861 tcaagattga tttttgcatt aggaataaga catattggta ctcatgcaag tgaaatttta
    16921 gcagaaaatt tttcttcaat tgatgaactt tcaaaaggta ctgttgacca attttgttca
    16981 attccagaaa taggacctac tatggcagaa agtattgttg aattttttaa aaataaagaa
    17041 aacttaaaag ttatagagaa gttgaaaaaa gcaggagtaa aaacatcaag ggaaaaaaag
    17101 gaggtagaaa aaaagaatat tttatcagga cttacttttg tagtaactgg aacattaaaa
    17161 aactatacaa gagatgaaat aacaaattat ataaaagaac ttggcggtaa tgtctcttca
    17221 tctgtttcaa ggaaaactga ttatattatt tatggagaag aacctggctc aaaattacaa
    17281 aagggacaag aattaaaaat aaaactttta actgaagatg aatttcaaga attagttaaa
    17341 aacctcctta caaaaaaatg ataaaagata ttcctatttc taaattaaaa gatgaatttt
    17401 tatcatatct ttatttagaa aaaaaccttt ctgaaaatac aatttcttca tataaaaatg
    17461 atatagaaaa atttactacc tttttaagag aaaaaaaata tagattaaaa gaaattgatt
    17521 tctcaaaatt tactgaattt cttttacacc tgaaaaaaaa gaatttatct gtttcatcaa
    17581 ttataaggat aatctcatca atcaggaatt tttataaatt tctatatgca agaggaatta
    17641 taaatgacta tttatttatt gaaacaccaa agtcagaaag ggacttgcca gaagttttat
    17701 ctaatgagga aatagaaaaa ttactttcta caacagaaaa ttcaaagaaa tataaaagaa
    17761 atatagcaat ccttgaactt ctttatgggg ctggtattcg ggtctcagaa cttgtgaatt
    17821 taaaaattga tgatataaat tttaatcaaa aatttatgag aataaaaggg aaaggcaaca
    17881 gagaaagaat tgcatttctt aatacagttg ctctacaaag aattaatgat tatcttcctg
    17941 aaagacaaca aactaaaaaa ggtaatcttt cgccatatct ttttgttaat aatagaggaa
    18001 ataaactatc aagacagttt atatggaaaa tagtaaaaaa atatagttca cttgcaaatt
    18061 tgaataaaaa tataaaacca catactcttc gtcattcttt tgcaacacat ttacttgaaa
    18121 gtggtctgga tttaagaatt gtgcaagaac ttttagggca taaaaattta tctacaacag
    18181 aaatttatac tcatattgat agaagacaaa taaagaaaat atacaaaaaa tatcatcctc
    18241 gtgcataacc atattgacaa atttttttta tgatttatta tatataaaaa aggtaactat
    18301 aaacattaaa aaaattataa aaaggaggca aaagtggctg cattaattgg aggtattgta
    18361 gcgattattg tgggaatttt acttgcagga ccaggacctc ttgggcaagc aactttatgg
    18421 tttattggtg gtggaattgt tgtttttctt gtattgggtg gaataatagc gattgctgct
    18481 ggaataagtg acataaaaga taagattgaa gaggaaaaag aaaagaaaaa agaaaaagag
    18541 gaaaagaaag aggaggagaa aaaagaagga gaaatagcat ctggaagtga agagaaaaaa
    18601 gaataagtat aaaatagaaa tatgccccga tttgtttttt acagatcggg gcttttttat
    18661 aagaatgttc agttaatgta ttagtttaaa acaaaaggtc aatatataaa aaacatcacc
    18721 tacaccaaaa aacttaataa atttgttatt ttgcataaca agtttttaga ggataaaaaa
    18781 aggccggggt ggaagataaa ttttaattac aaagaacaaa acaaatagaa taataaagaa
    18841 aaagttcttc cacattgggt tggttgtact attgcaatac aatatttcgc accaaccttt
    18901 ttgaaataag taagtatttt acttcataaa gaaaaacaaa aaaattacta tttgaaaaaa
    18961 ggagatgaaa tgagtaaaaa ggaaataaga gaaaattatt tagaaaatgt tattggaaaa
    19021 ttaaaatcag ttgaaaaaca ccaggtaaat aatttccgta aagcaggaga aatgattgca
    19081 gatgcttatc aacaagataa attagtgcat gtttacggtg gtggtgggca tacatgttta
    19141 atggtatgcg aaatgttttt tcgtgcaggt ggtttagcaa atattaatcc aattatggga
    19201 catgatatat caccattttg ccaagcatta aaatatttag aagtagaacg aacaacaggt
    19261 tatggtagat gtttaataaa gtattataga gttcaaaaag atgatattct tattattttt
    19321 cataacatag gagcaaatcc aacaacaata gatgcagcag acgaagcaaa aataattggt
    19381 gcgaaaatta ttgctgtttc ttcaaataac tggcgagaaa aattaccaaa aaatcatcat
    19441 atcaggcatc ctaataaaaa gcatcttttt gattatgctg acctttgtat tgacgatgaa
    19501 aatccctatg gtgatgctga ttattatatt gaaggattta atgtgccaat aggtccaaca
    19561 tccacaataa ttgatgccta tattgcacat agaattgtaa tagaaacagt aaagtccctt
    19621 atttcacgaa aaatagagcc acctattttt cgcagtgcaa atcttccaga aggtgatgag
    19681 tataattcta aattaattga aagatatcgt aatagagtta aggatttata attacaaaaa
    19741 aataaggata aaaaatgcaa aaaaatatag aaaaaaataa aaatagtaaa actcctgcac
    19801 aaaaattttt ggatttaatt cgtaaagata ttatagaaat tcaaaatgat aaagagagat
    19861 tgatagaaat ggctgaagag atgtcagatt cacttttaaa gggagggaat atttttgctc
    19921 cacctgtaaa taaagcatgg acatcagaat ttagtggaag agcaggagga ttgatgtgtc
    19981 ttaaaggaaa ggcaaatcac tcaaaggata tagcgttttt tgcattacct gaccctcgca
    20041 attgggagcc tcaaaaagat aaagtgctaa aaacattact gaaaggtaaa gcaaaacttt
    20101 tcgtaatagg aagaaaagaa gatttaggag atataaaaat aaataggcag ttcgctggtt
    20161 ttacaggtgg tgttagagca gatataggaa tgtatagata caaaaacttt aatccttttg
    20221 cttcaacaag agagtttaaa atttttgtta gaggatggat aacaacaggt gagatgatat
    20281 gcgcatgtat tcgtaaaggg aaaatgccaa ttatctatat gagtgtctgg tttgaaggag
    20341 caatacctcg caatagtagt tttacatata ctgatttttc aaattattat gaaccatctt
    20401 taaccaaacc aattgttcct ttagaagttt ttcataaaaa tcgttatatt cctcctttac
    20461 caccaggata tgctactact gaatttctcc aaatagtaaa aatgttaatt gataaaattg
    20521 ataatcaagc agatcaaatt tccagaatag gacaatgtct tgcagaagca aaacaaaagg
    20581 gaaaaaaaat tcatgttgtt gctgctggac attgctatcc aggaatatta gaattaccag
    20641 atgataattc atatccttta acatggggag tttcaagagc agatttaaaa tatgctgttc
    20701 cttctgattt aaaagatggt gatgtagcat tacattttgg ttatggtcca acaagtattt
    20761 ctgatgcaaa aaaaattctg aaacgaggag tgaaactaat tcatacatct ccatatggtc
    20821 ggcgaatagg tataccagaa aatgaaaatt ttttatggtt tgacctccca tggagacctg
    20881 gcgatgctgt cattgatata ccaggataca gtgtccgtat gttaccaagt agtagtactg
    20941 cacactcaat tgcttataat gcaattcttt gtgaaatggc agaagtaatg ggatggcagt
    21001 aatatatagt aatttaatat taagaaattt ttagatattg taataaaact aactatttta
    21061 atactatatt aaatattttc ccttttacat atactttctt tataatttct ttgttttcaa
    21121 cccacttaga aaatttttta tcgttaataa ttttttcaaa aagttctttt tcatctatta
    21181 ctgttggaac atttattgtt ccccttaatt ttccatttat ttgaacaggg actgtaatta
    21241 tatcttcctt ttctgttgtt tctttatatt caggccattt ttgataataa ataaattctt
    21301 tttcaccgtt tctttcccat aattcttcgc ttatatgagg ggcaaatggc gaaataatct
    21361 ttataaaatt tttccatgtc tctacaatta tttttttaga agcacttttt tctgatatta
    21421 ttttgctgaa aaaatttaaa aactccatta tttttgcaat tcccgtattg aaatggaaat
    21481 cattttcaat atcttctgtc actcctttta ttgttttatt tattgcaatt tctacttcat
    21541 tgtttttttc atttttctca ccttcataat ttttaatttc attgtttatc ctccaaactc
    21601 tgttgataaa tctccagcat ccttcgattc ctttttcact ccattctaag tcaagttcag
    21661 gaggtgcagc aaaaagtata aataatctta aagtatcaac tccatatttt ttaataatat
    21721 ctgttgggtc aacaacattt ccttttgatt ttgacatttt acttccatct ttaacaacca
    21781 ttccctggca gagtaaattt tcaaatggtt cctcaaaatt aatatatcca atgtctttta
    21841 aaaccttcac aaaaaatcta ctataaagaa gatgtaaaat agcatgttct atcccaccaa
    21901 tatattggtc taccggcatc cagtaattta cttcctcttt atcaaatggt ttatcttcta
    21961 ttttttcgga agcatatctt aaaaaatacc aggaagaatc aacaaaagtg tccattgtat
    22021 ctgtttccct ttcactttct tgcccacaaa taggacactt acattttaca aattcaggaa
    22081 tattttttag tggtgattct ccctttcccg taatttcaat attttctggt aatttaacag
    22141 gaagttgttc ttctggaaca gaaacagttc cacatttttt acagtatatt attggaattg
    22201 gagttcccca gtatctttgt cttgaaatac accagtccct taatttatag tttactgttt
    22261 ttttcccttt ccctttattt ttaagaaatt ctattatttt ttcttttgct ttttcacttt
    22321 cctgtccatc aaatgttcca gaattaacca atatcccttc tccttcatat gcattttcta
    22381 aattttctgg taaattttgc caatcagggt tttttataac aattttaatt gggatattat
    22441 atttttttgc gaactcaaaa tctctctcat catgagcagg tactgccata attgctcctg
    22501 ttccatattc cattaaaaca taatttccca gccagatagg aattttctct ccattaacag
    22561 ggttaattcc ataaatttct gtatcaattc cttctttttc tgtttttgag gcatcaataa
    22621 cttttattct ctgtttcttt gttctttcta caaaattttg tatttcacta ctattttttg
    22681 atttttgaat taatttttta accacagggt gaaaaggaga aacaacaagg taagtgcaac
    22741 caaataatgt gtcaggtctt gttgtaaaaa tagttaaatt ttcacctaat tgaggtatat
    22801 caaattcaac ttcaccgcca gtgctttttc ctatccaatt tttctgcatt gtaattactc
    22861 tctcaggcca gttttttatc ccagaaaaat caagcaatct ttcagcataa tcagtaatct
    22921 ttataaacca ttgttcaaaa tttttctgtt caactaaact accacatctc caacatttat
    22981 catccactac ctgttcattt gcaagaactg tctgacatga tggacaccaa ttagcaggtg
    23041 cctgtttttt atatgctaaa tttctcttaa aaaattggat aaaaaaatat tgattccatt
    23101 tataatattc aggagaacat gttattactt ctctttccca gcagtatgaa ataccaagtt
    23161 ttttcaattg ttgtctgata gtattaatat ttttatatgt ccattcccat ggttgaattt
    23221 tgtgttttat tgcagcattt tcagcaggta gaccaaaagc atcatatcca atcggatgca
    23281 atatattaaa tccattcata actttatatc ttgcaactac atccccaatt gtatagtttc
    23341 taacatgtcc catatgaatc tgggcagaag tgtatggaaa catttcaaga atataaaatt
    23401 ttttattatc cttttggaat tttgccttaa ataaattatt ttcttcccat tttctttgcc
    23461 atttttcctc aatattttca tagtcatatt tttccatatt tttctccaat tctcactcac
    23521 agcattttga agtttttgtt ttgaatatta tgtattattt atatgttaat agaatataac
    23581 ccaaattact atttttttca atttatttta catttattac ataaaaggta taataaaaaa
    23641 aatttaaagc caaaaaagga gggtattgtg aaaattgcaa ttcaaatagg actcttgccg
    23701 gggaatactc ttgaagaaaa attttataaa gcaaaagaga ttggatttga gggtattgaa
    23761 gtatatggaa gggatttact taatgatgaa aaaattgttg agaaatataa aaatttgtca
    23821 aaggaaatgg gtatccaaat ttcttctata tgtgccggct atagaaaatt tcttcttgac
    23881 ccaacacatg aggaaagaca acaatcaaga aacgaaataa aaaaattgtt agaatttgga
    23941 aattaccttg gagttgttgg gcttattctt gttccagttt ttggttcacc taaaataact
    24001 gacctatctc catacaaaaa tgcatttgaa cttgaaaaag aacttttaat ttctcaatta
    24061 ccagaattag tagaaaaggc agaaaaagaa aaatgtgcgc ttttacttga accacttaat
    24121 agatatgaga cacattttat gaatcgtatt gaacagggaa ttgaattatg caaaagaatt
    24181 aaaagtgaat atttaaaaac aatggctgat ttttttcata tgagtattga agaagttgat
    24241 atagcaaggg caattaaaaa aggtggaaaa cttatttcac atgtccattt agcagatagt
    24301 aatagaattt tgccggggat gggacatact gacttcaaaa aagggtttaa ggcattgaag
    24361 aaaattggat atgataaatt cttggcactt gaatgcggag ttcctgaacc aaaaaaagaa
    24421 aatttggaca gatgtttcca ttatttaaaa aatataattg aaactatata aaaaaggaga
    24481 aaaaaatgaa aaaattaaag gttggtataa ttgggactgg tggtattgct caggtttctc
    24541 atattcctaa ctttcagaaa attgaagatg tagaggttag tgcaatttca gatataaata
    24601 aggaaaaatt aaattatgta gcagaaaaat ttaatatccc aaaaacattt acagactggg
    24661 agaagatgct tgaagaagat attgatgctg ttgttatttg ttcacctaat tctttccatt
    24721 caattcagtc aattaaagca atggaaaaag gaaaacatgt tttatgtgaa aagccaatat
    24781 gtttaagtgt ggaagaagca gagaaaattt ttgatactgt tgataaaaca ggaaaagttt
    24841 ttatgggcgc tttcccaaga agatttttag gagaaacaat tgtcttgaaa aaaatgattg
    24901 ataatgggga ctttggtgag atttattatt taaaagcaag ttatttaaga aggagaggta
    24961 ttcctggact tggaacatgg ttcacaaata aaaaacttgc tggtggtggt cctatgatgg
    25021 atgttggggt tcatgtgatt gatatggttc tttatcttgc taatttaact gacccgaaaa
    25081 ttattattgg gacaacattt gagaaattta aagataaagc agtagatggt ggttggcctc
    25141 caattgaaac aagaaaagga gaaaaggtaa ctaataaaat ggatgtagaa gacctatctt
    25201 gtggttttgt taaattttca aatggagcaa cactttttgt agaagcaagt tgggcgggaa
    25261 attcagaaac gggactgaaa gcaagtttgt ttggcacaaa aaatggtgca caattgcctg
    25321 accctgaaaa taaagaaaat ccggtaagaa tatattctga aattgatgga attatttctg
    25381 atattactcc tcaaatacca atattagacc catatcttga agaggcaaaa cattttattg
    25441 aatgcataaa agaaaataaa tcccctatta ctaaaaagga agaaataata agtgttgtta
    25501 aaataattga aggtatttat aagtcatctg aaacaggtaa ggcaataatt tattaaatat
    25561 cagatggctt tttaactttt tattaaacac aattctcttc ttttactgaa aattgttttt
    25621 ttttaaatga aaaaagggat tgaagaaaaa gtaattaaaa agagtactgg ggttccaata
    25681 cttataagag aggatattga gtatttagat aaaggacatt ctgtttttct tgcatatcac
    25741 caagaagttg aaattcatat aataaaagaa ggaaaagggt tttatctcat tcaaaataaa
    25801 aaatatattt ttgataaaaa tactgcaata attatttatc ctgaccagca acatcttcat
    25861 aatatttatc caccttatag tattaaaaaa atttctcttt atttcaatcc cggcatttta
    25921 aaaaaaatta attttgacat aaattcttta ccacatttaa taaaaatacc tggtaaaaac
    25981 atagcaaaaa ttcaattagt tgttgatttg atgaaaaatg aaatatctga gaagaaaaaa
    26041 ttttgcgaag atacaacata ttctttactc aattttattc ttaatttatt gaaaaggtgg
    26101 aaatacgaaa aagaaattcc ttataagcaa gcagatccac taattgataa attaatagaa
    26161 tatttaaatg aaaattatta taaggatatt gacattttac atctttcaaa aatattttat
    26221 aagtcagaaa gttatctttc acacatattt aagaaattta caggtatagg aataaaagaa
    26281 tatattattc aaaaaagaat tcttgaatca aaaaaaattc ttgaaaatca aaatgacatt
    26341 aaaattacaa aagttggaga gaaagttggt ttttcttcat ttccactttt ctatcgtgct
    26401 tttaaaaaaa taacaggtat ctctccttct tcatataggg acattttttt aaaagcagaa
    26461 aataatataa agacagcaga aaatataatt taattttaat tcttttaatg tataattaac
    26521 ttactgaggg aaaaaatgga gaaaaaattt agttggtatg taagtggttg tcatgaagca
    26581 tatgcttatc tttctggatt aaaacttgat gaaatttata aagattattt ttcttgtgca
    26641 aaagcatttg aggtaggtag aaaaaaaatt aaagaaatat ttggggagca ggtagtagtt
    26701 ggttctcctt cctgtgctcc aatttcttat ggtcatataa tatgtttggg agttcctgta
    26761 agttttccag aggactcgga accaggtgta aggccaattt ataaaaatat tgatgaaggt
    26821 attgacgcat taaaaaaaga tgttgatttt agcaaaaatg aattatttaa acattattta
    26881 ggtatgtggg aatatttaaa aaataagttt cctgatgaaa aagtttattt ttctggattt
    26941 ggatgggaag gtccaataac aacagcagtt cttttaagag gaactgattt ttatatggat
    27001 atttttgatt gtccagaaaa agtcaaaaat tttctttctc ttgtaacaga gagtgttata
    27061 aaattcgttt atttcacaag aaacataaat aatgagccac caataaattc tgaaagtagt
    27121 gggttagccg atgacctttc aagtttaatt tccccttctt tatgggatga atttgtaatt
    27181 ccttattggg aaagatatta tagtggttta accactggaa aaagaacaat acatgttgaa
    27241 aacttaaaac cagagcatct tccttacctt aaaaaagtaa atatctctta ttatgaccct
    27301 tctgtatctc ctgctttaag tccaaaaata ataaaagaaa aaatagatat accatttgaa
    27361 tggcgactcc cttcatttca attaagagat atgaaaaaag gagatgtaag aaaatggatt
    27421 tatgaaatga taaaggaagg ggcagataca ttatttcata ttatagggaa agaatgttgt
    27481 ttaggtaata atcctgataa agtaaaggaa ttcattaaaa catgtaaaga aatagaaaaa
    27541 ggagaagatt atgggaaaat tagcaattga aggtggaaag ccggtaagag aaaaaccaat
    27601 gccaggaaga attatgtttg gtgaagagga aaaaaaggta gttatggaac ttattgaagg
    27661 tgggataaaa ggaacaacag aaaaatttct tgatagatat gcaggagaaa atgttaataa
    27721 atatgaagaa gaatttgcaa aattttttgg tatgaaattt gcaacagcag taagttcagg
    27781 aacagcagca gttcattctg caatcgcgtc tcttgattta gagccaggaa gtgaaattat
    27841 aacaagtcca ataacagatc ctggaacagt catgcctatt ttatttcaag ggcttatacc
    27901 tatttttgct gatgttgatt ttgatacatt gaacataaca tctgaaacaa ttgaaaaagt
    27961 tattacaaaa aaaacaaagg gtataattcc agttcattta gcaggagttc catgccagat
    28021 agacaaaata atgaagttag gtaaaaaata taaattgaaa gttattgaag attgtgcaca
    28081 agcacatttt tcaatttata aaaataaata tgtagggact tttggtgata taggtgtttt
    28141 ttctttaatg agtggaaaac atatgacaag tggaggacag ggtgggatgg ttataactaa
    28201 caatgaaaaa tattattgga atgcaaaaag gtttgctgat agaggaaaac catttgggat
    28261 aaaaaatcca aaaggaaata tttctattgg tttaaattat agaatgacag aattagaagg
    28321 tgccattggg agggtccagt taaaaaagat aaaggtagtt gcagaaaaac gatacaaaat
    28381 agttgagaaa ttaagagatg gtttttcatc tttcaaaaca ttttctttat ggaaaaagcc
    28441 cgatgaaagc aaaccaaatt attggttttg ctttgtttgt gttgatgata aaaaattaaa
    28501 agttgataaa tggaaaacag cagaagcact aagagcagag ggtatgccag taggtgctca
    28561 ttatgttatt cccatgtacg aaaatccatt tattaaaaat aggaaaacat ttgggaatag
    28621 cagttatccg tttaatccat ttaatccaaa tattaagtat aaatgtcctg ttgcagaaaa
    28681 aacattaaat tcacatataa ctttatacat acatgaatgt tttacagata aagaaattga
    28741 agatatatta aaggcatttg ataaagttga aagtgcctat ctaaaatagg aggaaaaaat
    28801 gatatttcac atgtttagag ttgattatcc tgataattgg gataaattaa aagaattaaa
    28861 tttagaaaaa ttggcaaaaa ttaagaaaga gttatacaaa gtagatattg agtgggttgt
    28921 tggttcattt ggaacatttc ctggacaggg atatatggta agttttaata caccatattt
    28981 tgagaagtat cctggacttg gtgattttga ttatataaga gaatatctcc cttacgcaaa
    29041 aaaatatgga ataaaattgg tatcttattt aaatcttcac tggttttctt atgaatttgc
    29101 ttcaaaacat tatgattggc aacaaataac ttatgatggg aaaatttatg gggaaaaata
    29161 tcctttatat ggaaatggaa ctacattttg tataaactct ccttttaggg aatgggcgtt
    29221 aaagatgatg gaagaagtta tgaaaacagg aatagatggt gtttttcttg atggtcctgg
    29281 aatttttcct gaaagttgtt attgcgagta ttgtagaaaa aaatttaaag aagaaaccgg
    29341 ccatgactta cctgaatggg aaaattggtc tgaccccatc tggaaggaat ttttactttt
    29401 tagaaaaaag tcgtttttaa agtttatgga agatgcaaag aaagttgtaa aaagaataaa
    29461 caaagatggg attattttta taaattcttc acatcttcct gaatggggaa ttccaagata
    29521 tattggggaa cttgaacaat atcaggattt taacctctct gaggcatttt atcatttagg
    29581 gagtgttaga gattactttt tttattattt tgttgcaaaa tacctaaaag tgagtgaaaa
    29641 accatcttct gctgcaatgc atcatgctgc tggtatctgg cattggatac ctctttatcc
    29701 ccaagaagta aaaatgagta taagtcaaaa tttatgtgtt aaaaattgtt tatggactgc
    29761 tgttttaaat ggaaatgata ttgaaaatcc agagtactgg caaccaataa aagaagtaat
    29821 ggaaaaagtt gaggataaaa aggaaatttt tgataaaggc aaaacttatg caccagttgg
    29881 attatatgtt tcttcttcca ccgtttattt ttatatttca gaaattaaag gaatatacac
    29941 agaagaagaa aaggtaaaag aagaaaatct tgttttaaaa agagagaaag aagttaagaa
    30001 agataagaaa cataaatgtg atgatataac atataaagaa ttgagtggat tttttgaagt
    30061 acttgttcgt tcccatatac catttaaaat aataaatgag aatatccttg aagaaaatct
    30121 caaaaaatta gatgttttaa ttttaccaaa tactacctgt ctttcagaaa aagaaatttc
    30181 tttaattaat gattttgtga agaaaggcgg aaaattaata atgagttttg aaagtggatg
    30241 gtatgatgaa tatggaaaca aaagagaaaa aaatccattt attgattttg aaatagaaga
    30301 taattttcct ttaagaaacg cagagcaata ttgtatattt gaggtagaaa caggaaaata
    30361 taaaaaagga agattagttc caagaccttc ttattcatta aagataaaaa caaaagagaa
    30421 agtaattctc tcatttataa aaccatcaaa agggacatat tcctattgta tagagaagtc
    30481 agattatcca gcaattatta aaaaaatcga agggaaagga aagataattt atttttctaa
    30541 tcttcctgga gaattttata attcatttca tcctcttgaa tgggaaaata taataagtga
    30601 aataataaaa gagaaaattg aatttgaaat aaaagttgat gctccttcaa ctacaataac
    30661 tgaattttat aaagatagtg ataactggat aatacatatt gttaattcaa caggtgatat
    30721 gaaaagacct gtttcagaaa ttattcctgt tgatgtaaaa atagaaataa aaggtaaatt
    30781 tgaactttct gaaagagttt ttgaagatgg aaatttaaaa attgaaaagg aaaaagacaa
    30841 aacaaatata acacttgaag gcatttctct ttatgaaatt atttatttaa ggagagcaga
    30901 atgaaagtat atataatgac tgatttagaa ggagttgcag gagtagttga ttttgaaagt
    30961 caaacatata acacaggaaa gtattatgaa atagcaaaag gactattaac agaagaagta
    31021 aattcctgtt gtgatggatt agtagaggta ggggttgatg aaatttttgt tagtgatggt
    31081 catgggtctg ggggaatatt gcctgaaaaa atacaccgag aggcaaaact tttacatgga
    31141 agacctcttc cgaaattttg ggaaattgat aaaaaatggg attgtatttt tctactatct
    31201 caccatagta tgcatggaac caaaaatgga aatttaaatc atacatattc aagcaggtca
    31261 atagtaaaaa tggaattaaa tggagaaaaa ataggagaaa taggaattgg agtatatctt
    31321 gctggttggt ttaatatacc ggtggttttt atcagtggag atgaagcagc atgtgaagag
    31381 gcaggaaaat atgttcctaa tatagaaaaa gcaattgtaa agtggggaat taccagaact
    31441 tctgcaattt cactttctcc aataaaagca agagaaataa taaaagaaaa agcaaaaaag
    31501 gcaatagaaa aaattgataa aataaaacca gtaaaatttg agggagaatg tgaacttctt
    31561 attgaattta tttctacttc tgatgctttt ggttttgtga aaagaccttt ttttgaaaaa
    31621 gttaatgaaa caacagtaaa agttaaaggt aaaaactttc ttgaactttt tgaaaggtat
    31681 ttttattaag tggatataaa aatgtaataa agacaaatgg aaaaatgaaa ttagattaat
    31741 tagaaataga aagctatctt acaacactta taataataga gaattgaatc agtgataaaa
    31801 attccagatg agatgatacg ataaaagtca aaaagtggtg aatactctgg gataagtagt
    31861 aaaataaaaa gaaatcctgg agtagatagg ttataagtga atttttcctt ggggcgaaaa
    31921 aaaggacctc gtaaaggaga ttaacaggca cctataactg aatattcact acaggatatt
    31981 agagcctgaa taggaggatg gtgttcacca agatatatct ctactccgaa ggataacaaa
    32041 aaatgcagga cagaatggga agatatatcg gagtggattt gcataagagg agttttgcag
    32101 tatgttatag aagtaaggca ggggagatag aagagaaaga gtatagtatg acggaggcag
    32161 ggatagagga attcaaaaag aggataaaga agacagatga agtaggtgta gaagcgacga
    32221 ggaatacagg atattttata ggagagatag agaatagtgt aggtagtata aaagtaataa
    32281 atccgacaca atttaaaata atagccaact cagtaaaaaa aacagacaaa atatctgcca
    32341 agacaatagc gaagtattta agtaaggggt taattccgga ggtaaggatg agaagcaaag
    32401 agcagatgga gacactgggt ttaataggga cgagggacaa attggtgaaa ttaagaacat
    32461 cattgaagaa caagatacac aatatattaa atgctaatgg gataatcacc aagaaagaga
    32521 tgttcagttc agataaaggg ttggataaga tattagagat agatttagat gaaggatatt
    32581 tatttgaatt aagattgata gtagaacagc tacatcattt aaacgagaca ataaagacca
    32641 tagacaagga attagccaag agagggaaga aaatgaaggg gtttaagaat ataacatcaa
    32701 tgacaggaat aagtaataca tcagggacaa ttttattaag tgtgatagga gagaaaaatg
    32761 attttgaaag agataagaag ttatcagcat ttttatggtt agttccgatg gtatatgcat
    32821 caggggatac agttcgctat ggtatgataa ctaaaatggg taataagata gggagaacaa
    32881 cagtggtaca atcaacattg gtagcaataa aatataatga gtatttaagg aatttttatt
    32941 taaggttgaa agcgaagaaa gggagtggta aagcaataat agcaacagca aggaagtatt
    33001 taacaataat ttataaaaca ctgagatata atttagtttt tgaggatatt aatcatttta
    33061 agttagcaga aagtagttaa aaaataactt gttggagtag agttttttga ctcttaacat
    33121 aggaagataa gatggaatgg tggaaaaaag aacctttaag agtaattgaa attgcacatg
    33181 gatatgaaat taatgaaaaa atgaaggtgt taaaagaatt gaatgcaaat atggagcacc
    33241 ttacaagata caatgcttcg gagttgtttc catcagaaga atttagtctt catagtggaa
    33301 ttttattaaa acctgatgat tttgataaat acttaaaaga agcacataca attggagtaa
    33361 aagtagtatt ttattataat gttcatgctg taaatattca atatgctaaa aaacggcctg
    33421 attggcaaca gatttgtgaa gatggaaagg caattgataa tgtatatgaa acatattctt
    33481 ctttttgtgt gaatagtggt tggagaaatg aagtatttga aatgttaaag aaaatttgta
    33541 aatttggatt tgatggaatt ttttatgatg gtccgatatt ttttgctaat acttgttatt
    33601 gcgagagttg taaaagatta tttaagggaa aatatggtta tcaattgcca caaaagacat
    33661 ccttaagtaa agatagagaa aattctcaat ggaaagatat aattgaattc cagtcagatg
    33721 ctattgagag atttttagaa gattcaaata atgtaataaa aagtgtaaat ccatcagttc
    33781 ttttctatat gaactgcaat actcttgctc caacatggcc aacaggaagg aataatagaa
    33841 aaataataag aaaaacagat attcttggtg cagaaggcgg atttttatat tatgggttaa
    33901 atgaaccaat ttataaacca ggtgcaactg caaaatttct aaattctcag gcatatggaa
    33961 aaccagcagt agtattttgt gcagggaaac aatgtccctg gaatttttct attttaccaa
    34021 aaggagaaat tacacttttt tatactcaaa caataactca tggagcaaat gtctggcttg
    34081 ctttatgtgt agatgaagat gtaagaaaat ataaacaaca ggtaaatata ataaaaagat
    34141 ttaataaatt tataaaagaa aatccatccc catttattga taatacaagt tcatgtgcaa
    34201 aaacactttt aatttttcct caaaggagtt tagattttta tgagggttca tcagttccat
    34261 taactgattt tactaaagaa ataaaaggag aaaaagcagg gaatttaatt gaggaatttt
    34321 atggctttta tgattctctt gttagagtcc atattccatt tgatgttagt gatgaggaag
    34381 ggattttaaa tattgaaaac gataaatatg accttataat ttttcctaat gctgtatctc
    34441 tggctgataa agaagttgaa aaaatagaag gttttgtaaa agatggcggt aatataatat
    34501 cttcatttga aacatcctca cataattatg gtaagagatt agaaaaattt aaacttggca
    34561 aaattttagg tatagatgac attggtaaga tatttggtcc attaaggtgg gattctattt
    34621 ttatagatgg aaaaattatt ttctctcccg aatatggaat tgaaataaaa acaaaaggga
    34681 aaataatttc ttattttgca aagcccttac ctggctctta ttcaaaaaga ccagaaattt
    34741 cagaaattcc atttatagtt gaaaataaat atgggaaagg taaatcaatt tattttgcag
    34801 gaacaattgg ctcttcaata tataaatatc attttcctga atattataaa ctcctttcaa
    34861 gtttttataa ttcattaagt agaaaaattt tagaaataga aaatgcacca acttctttgg
    34921 aaatttcaat aagagagaat gaaaagtttt attatatcta ctttataaat ttcacatctg
    34981 aaatgaaaag accaatagag gaaataatgc ctataagaga tataaaagtt aaatttttta
    35041 caaattcaaa aattggagat gtttattctt tatggaataa agaaaaactt acttttaaaa
    35101 aagcaaaagg aaagataatt tttaatatac caataattaa agaatatgaa ataattaggg
    35161 taaaaaagga ggaaaaatga ttgattttca tacacatatt ggtaaatgtt tttatggaag
    35221 gaaaatactt actgggaaaa aattagttaa gaaaatggat gaattaggaa ttgagaagtc
    35281 ggttatattg cctattgaaa atccagaaga aacgcaatgg tattctacaa ctgattatat
    35341 tttaagggtc tctaaaagat ataaggaaag attaattcct ttttgtaatg ttgacccaag
    35401 gagaggaatg aataatggag aagacccata tattggaaaa atgattgaag aatatgtaaa
    35461 aaagggatgt aatggatttg gagaagttct tgcaaatttg gagtttaatg acaaaaggat
    35521 gaagtatatt tatagaattt gtggtgagtt aaaactacca gttctttttc atttaggtgg
    35581 agttccaggg aagtcaacaa ttggtttaac tgataaactt ggactaccat ttatggaaga
    35641 agtgttaagt gaatttccag aaacactttt tattgcacat ggtcctggct ggtgggcaga
    35701 aatttcctca gatgtaaaag aaaaagatag agatggatat ccaaaaggaa aaattgagaa
    35761 agcaggaaag gtctgctatt tacttgaaaa ttatcccaat atctatgctg acttatctgc
    35821 tggcagtgca tataatgcat taacgagaga taatgacttt ggaattgagt ttttaaaaaa
    35881 acattataga aaacttctat ttgcaactga ttatctatct gttggtcagg aacttgaaat
    35941 tgtagattat atgaaaaatg tgaaaattcc agaagaaatt aaaaaaatgg tcacaaaaga
    36001 aaatgcaaaa aagattttga aaatggaggg atgaaatgga aaaattggca attttgggag
    36061 gagagaaagc agtaaaaaat gagaatgaag aaatttttaa atggcccata gttaataaag
    36121 aaatggaaga taaggtatta gatgttttaa gggcaggaaa aatgtcagaa acagatataa
    36181 caatggaatt tgaaaaagaa tatgcaaatt ggtatgggat gaaatatgca ttagggacaa
    36241 ataatggaac tgctgcttta cattgtgcat tttatggaat taggattggt ccaggagatg
    36301 aggttatatg tccatcagtt acctattggg cttcatgtat acctgtctta tcacttggag
    36361 gaacagttgt ttttgcagat attgacccgg aaacattaag cgttgaccct gatgatatag
    36421 aaagaaaaat tagtgagaaa acaaaatgta taattgttgt tcattattgt gggtatccag
    36481 cagatatgga tagaattatg aaaatagcag agaaatataa attaaaagtt gttgaagatt
    36541 gttctcactc acatggttcc ttatataaag ggaaattaac agggacattt ggtgatgtat
    36601 ctgctttttc aataatgagt ggaaaggcat ttgcagttgg agaaggtggg ataatgctaa
    36661 caaataacag agaaatttat gaaagaggga ttgtttttgg acattatgaa agacataatg
    36721 aattaacaat tgactattta aaagatggag cagggcttcc gtggggtggt tataaatata
    36781 ggatgcatca gatgtcatca gcggttggtc ttgttcaaat aaaaaaatat aaaaaggaga
    36841 tggaagaaat agataaagca atgaattatt tttgggattt acttgaagga gttgctggta
    36901 tcagagcatt aagaccgcta aaaaattcct ctcttacgat gggtggttgg tattctgcaa
    36961 agggaaaata ttatagtgaa gaattaggtg ggttatctat tacaaaattt tgtgaagcag
    37021 cgagagccga aggagtacct ttaagtcctg gatgtaataa accattacat actcatcctg
    37081 tttttcaaaa catagatatt tataatgaag gtaaaccaac aataatatca aaagcagcaa
    37141 gagatgtaag aaaattatat aaatcactgc ctatatcaga aggtataaat aaaaaagtat
    37201 tttcagttcc atggtttaaa aaatttgtac ctgaaattat tgaacaatat gcttatggaa
    37261 taaaaaaagt tgtaagtaat tataaggaat tgttgaaata tgatgaaggg gacccggaag
    37321 aaattggtgg atggagttta acattccata ggtaatttat aatacaagtt gaaaaaacac
    37381 aaataaaaaa ggaaaaagta gcaggataag aagataaaaa ataagtcatt aggatgttgt
    37441 tatggagtgc ttgctactga aatataagat gcttaggtag tgtcaaaaat agatgaaaaa
    37501 gagatgaaag agaaaaaaca ctcttcaaat ccccctcaat ccccgttgtg cctattgaaa
    37561 tacattacag caccaactct gtgtgctaaa ctttgcaact ttagtcagtt tactaaaggg
    37621 ggaagtaatg agggaaatac tccttccctt tataagggaa ggagggctcg cccgtagggc
    37681 tatgcccgag gggatggatt tcaacttttc acatttcacc tgtctttgtg agaaaatgta
    37741 aggtgaattt ggggagataa ttttggcaat acccaaataa aaagcaggag agaaaaaatg
    37801 aaaatatata taatgactga tttggaagga gttgcaggag tacttgattt tgataattgg
    37861 tgtagtcctg aaagtagata ttatgaggta gcaaaagagt ttctaacaat ggaagtaaat
    37921 tcagcagttg aaggattttt tgaaggagga ataaaagaaa tagttgttgc tgatgggcat
    37981 ggtccaggtg gaataaatcc aaaacttctt gataaaagag taaaactttt aagaggatgg
    38041 caaaagtggc cattattatt ggataagtcg tatgattttg ttgcttttgt gggacaacat
    38101 gctaaatcaa gaacagaatt tgctcatata gcacatacac aaagtttttc atattttgaa
    38161 atgagaataa atgatatagc aattggagaa tttgggcaat tttctatgtg tgcaagtgaa
    38221 ttgggagtaa ggacaatatt tgcatcagga gataaagcat tatgtaaaga agcagaagaa
    38281 cttattcctg gaattgtgac tatcactgta aaagaaggaa ctacaccagg aaaaggtgat
    38341 gagtgtccaa gagaagcata tcgcagaaga aataatgctg ctattcattt acattcagag
    38401 aaagcaagag aattaataaa aaaaacagcg tataaagcaa taaaaaaagc aattgaatat
    38461 ccggactggg gaattattcc gttaaaagcc ccttttaaaa gagttgttat atttagacca
    38521 gaccaagatt ttggtaaaag aatatcaata gaggaacatt caagtagtgt tatagaggta
    38581 atgaacatgc cttataaccc aaaaaactat gatgaaattt aataaaataa atggaatatt
    38641 ttgaaaaaat attagaaaac gctttatttt tattaatagt aataaatcct gtaagtaaaa
    38701 ttttaataat aaattcatta atagacgaag gaaaacaatt tataaaaaat atttcagtta
    38761 agtcatctta tacaggactg ataattctta taatttttgc agttggaggt aattttattt
    38821 taagaaatat ttttagaata aacatttatt cccttcaaat agcaggggga attatacttt
    38881 ttctctttgg actaaatgcc ttattaaaag ggggatttgg aatagatgag acaaaagata
    38941 cagaagaaat tgcaatgatt actcttgctt caatgatagc aggaccaggg acaataactg
    39001 caactatttc tttatctgct gtttatggag taatttcagt atgtatttca attattctgg
    39061 ctgtctttat aaattttatt ttgatgatat attcaattga aataggaaag ttattaggaa
    39121 aaaatttatt aggtccactt gtaagattaa ctggtttttt tatctcaagt attggaatga
    39181 atatggcttt tactggaatt aaggaatttt taaaataaaa agaagcatca aaacttttga
    39241 aaggagatta aaatggaaga attattatgt ggtataagta aagtagatat aactccaccg
    39301 gttggatatt atcttcaagg tcattcaggt agaaataagc catctaaaaa agtccatgac
    39361 cctttatatt taaaattagt ttgtctttcc gataagaaaa cgacattata tattttaagt
    39421 tcagacctta ttggtttttc tgaagaattc gtccataata caagggaaga attaaaaaat
    39481 aagttaaaaa tatctcctga aaattttctt tttacttcat ctcatacaca tacagggcca
    39541 tttattgaaa gcacttatgg gggtaaagat aaagttgacc ctgactactt aaaaattttg
    39601 cagaaaaaaa tagtcggtgc tgccattgaa tcaagaggaa atatggaaaa agtaaatgta
    39661 agggtaggaa aaggcaaggt taatattggc attgttaaca gaaggaaaaa aacaaaaaaa
    39721 ggtgtatata tgatgccaaa ttttgaaggt ccaattgatg aagatgtctc agtaataaaa
    39781 tttgagaaaa aagatggaac ttcaaaagta attttattta actatacatg tcatccaaca
    39841 actttatcaa ctaaaatata tgaaatttct gcagattatc caggtgtagc acaaagggaa
    39901 attgaaaaat cttattcggg ctgtcttgct atgttcacaa atggatgttg tggagatgtc
    39961 agacctgctc ttgttgaaaa tggtcaattt atagggggaa attttgaaga tattgaaaga
    40021 atgggaaaaa ttttatttgg agaggttata aaagttagcg aaaaaagtat atcactaaaa
    40081 gaggttaaat tgaatagtaa aattgttaac tttaaatttc aacttgaaga taaattaata
    40141 cctaaaaata taaaacatct tgaagagatt gttcaaagtt ataaaaaaca attttcatat
    40201 tttggacctg atataataga ggagtggaaa gaagatatga gaggaagaat tgaaaaggga
    40261 gaaaaattaa ataattacat tgaaggttat ttacaattta tcaaaattgg ggatattttt
    40321 attttaggat taccaggaga aattatggta gaaataggaa ttaaattgaa agaaaaaata
    40381 gggaaaaatc ttattatatt tggctattca aatgggctaa taggttatgt tccaactgca
    40441 aatggaataa aagaaggtgg atatgaagca gggtctttcc tttatcataa atatccagca
    40501 ccttattcag aaaaaatgga gagcgattta ataaatgaag taataaatca gtttaagaaa
    40561 ttttgaaaat tcagataata ttatccaatt acaatagtta aaaatataag tgcatagaat
    40621 ggaaagaaag ggcattgctt ttctgcaaaa aaaacataat aaattgtttg ggaaaaataa
    40681 gtcatatctt ataaaagtgc cattaagaat atcccctctt ggtgctcata ttgaccatca
    40741 attaggaatt gtttccggga tgactattga taaatatata ttttttttat ttagcaaaaa
    40801 caatagtaag aatgttaaaa tttatagtga aaattataat gactttgttg aattttcaat
    40861 aaataaaaat tttaaaaaag aaaatagatg gtccgattat ataaaaggtg ctgctcatat
    40921 tcttaaagaa aaatttagaa tcaaaaacgg tataaatgga gttgtttatg gagaaatatc
    40981 aacaggtggt ttaagttcct ccgctgcaac cgggatttct tatcttcttg cacttgaaaa
    41041 agttaatgaa attgtagttg ataaaacaga aaatatctca ttgatgcaaa aaatagaaaa
    41101 tgaatttatc ggattaaata atggaatttt agaccaatca gttatactat taaattcctt
    41161 acatccgcaa tctctaattt ttattgactg caaatctgga aaatatagga atgtaattcc
    41221 aaagaagaaa tttgactttc aaatacttat tgtccactca ggggttaata gaaccctttt
    41281 aaatacaaac tataatgaaa gagttagtga gtgtgaagaa gcaggaaaaa tacttttaaa
    41341 atttgcaggt aaaaaaataa aaaaaaatat aaaattaagg gatgttaaaa aagaatattt
    41401 tgaaaaattt aaagataaat tacccgaaaa cttaaagaaa agggcaacac atttttattc
    41461 agaaattgaa agagttaaaa aaggagttaa atactgggag aaaggagaaa tagagaaatt
    41521 tggcaaatta atgaaaatgt ctggtgaaag ttcaataaaa aattatgagt gtggaatacc
    41581 cgaaacaatc caattacatg acattttaaa ctctattcca gaaatttatg gagcaagatt
    41641 tagtggtgct ggatttggtg ggagttgtat agcattaaca aaaggtaata ttgaaaggga
    41701 acaaataatt gtgaacattg aaaaaaaata tataccaaaa tttcctgagt taaaagataa
    41761 atacaagata ttttttacta atcacgggag aaaaccagaa atatttgaat tataatataa
    41821 ttactccatc catattaact ttgttttact tcttttttgt ataattggtc aatatcagga
    41881 actttaccat tttttagttt gtataacact tcccctgctt tttcaactac aagtattcct
    41941 tcattttccc tgtttttata attttctctg tccagagtat caggattaat atatcccaaa
    42001 tttattttcc ggcatatttc ttcacttatt cctgttgcaa gaattacttc aattctcggt
    42061 ttttcaattc catctataaa agtccctatt cctttaacat gtgttgaatg agcaagaata
    42121 caaccaggat aatttttaaa tttctcccat tgttttagaa aataatctct tgtatgataa
    42181 ccaacttctt ctaaatattt tccgtgagta tatgacacaa catttatatg aggagcaaaa
    42241 ataataagtt ttcctccatc agcaacaact ggttcaagtt tatacataca ttttcctgct
    42301 gtccatatat catcatacat aggtggtgca actgaaatta ccatattaaa tggcatatca
    42361 acatatttta tatttatttt tgaagaaagt tgtgctgctt tttcccagtc atctgtatca
    42421 ccagcaaaaa caccgtaaat taagttttca tttactacat agtttattaa atgaactttt
    42481 ttatttataa atgaagctgc aatatttatt acatctcgga caggtgtttt tattactcca
    42541 tttattttag gatttgttat taaagctcct aaccagtgga atttatgtaa aaattcagga
    42601 ccgcaaattc ctggaaaaaa atacttataa ccacctgaaa aaccaacaac ttcatgggga
    42661 aaaacaggac cggaaataat tatttcatca tattgaaaga tttttctatt tattctaaca
    42721 ggaatatttt catttaaaag ggtgcttata tattctccca tctgttgttt atttattata
    42781 ccgatttctt caagtgcctc tgaattattc cactggtgct ggaaaatttc tgtttcttca
    42841 tttcctttta tatctaaaaa ttctcttatt ttttcatcag acatttctgg atgtgttcca
    42901 agtgcaatca taaaatcaat tttttttact ttctttttta gaatatctaa aattgaatga
    42961 aaaaaaagag cagttggtcc tgaccttgtt gtatcaggaa ttatcacaag gaccttttta
    43021 ttagaaaaat cattttcttt tgttaaataa ttttccaaaa tttttcttat ttcctcttca
    43081 ctaattattc tatttacctc tcctttacct gttcccatat ctacctcgtt tttatttaat
    43141 atggtacttc caaaaatctc gtcttatttg caatgttttg tggttaatct ttgggcggat
    43201 ataaaaatcc ccctcactcc cgttgtacta tctgaaggac aataacacac caactctgct
    43261 ttgctaatca tcccctcttt tgtcgggctg tcaaaggggg aacatttgtc tccttccttt
    43321 ataaaaggaa ggtggggatg gatttcctgt aatttcctta aatccccctc tctccccgtt
    43381 gtgctatctg gaaaatctcc cctaacccct ctttacgaaa gaggggtgta ttttggaata
    43441 acgcaccaac tctgcttttc taccctttgc ttctttagtc ggtttgttaa agaggaaata
    43501 caccttccct taagagaagg aatgctcgcc cgtaagctcc tcgcttttag ctacgcatcc
    43561 taatttattt agttatttct ttttcaggtc ttcctggtaa taaccactca gtatgaaatt
    43621 cccttatttt tccattttct gtttctaata tctcaggact ttcttctccg ccaattttaa
    43681 aatatccatg tgctccaaag taatctctct gtaaagcagt tacctgacag gcaaacataa
    43741 ttggtgaagt taattgtaaa atataatcat aggaatttgc caatcctaat gcaggaactc
    43801 cgactttatg agaaatttca accaactttc caagtttcca taaattcttt ttaataaaat
    43861 cagaaaattt actgtgtgca atcaaattta aaagttcagg attctctaaa tatgcctttg
    43921 taatttcatc aagaaattgt gctcttataa tgcatcctgc tctccatatt tttgctattt
    43981 ctcctaaatt taagttgaat ttataaaact cagatgcaga tttaagaagt gcaaaaccct
    44041 gagcatatga agaaatttta cctatataaa gtgcatcatg agcaatatca ataatatctt
    44101 cctttttctc atttagttct tctctgatat taagaggtag aattttactc attttcaatc
    44161 ttaaatcttt ttccccagac atttctcttg aaaacacagc agaagatatt gttggaacag
    44221 gaacaagtaa ttccaatgca ctttgaactg tccatgttcc agtccctttc attcttgtaa
    44281 tatctgccac aatatcaaca agatatactc cttcgtttct tttatctttt tctttcaaaa
    44341 ggtccgatgt aatttctata agataagatg ataatatatc atttttgtta ttccactttt
    44401 ccataatttc accaatttct tcgcctttca tacataaagc atttttaaaa aaccagcaaa
    44461 cttctccaat aagttgcata tctccatatt caattccatt atgtgtcatt tttacaaaat
    44521 gccctgctcc accaggacca ataaaagata cacatggaga accatcatac gcttttgctg
    44581 aaatatcaga aaacattttt tcaataagtt gatatgcttc ttttgaacca cctggcataa
    44641 tagaaggacc ttttaaagcc ccttcctctc caccagaaac accagttccg ataaataaaa
    44701 ttcctttttc tgttaaatat tcatatctcc tgatagtatc tttaaaatat gaatttccac
    44761 catcaattat aatatctccc ttttcaagaa atggaataag tttttcaata aaatcatcaa
    44821 ctggttttcc ttcttttacc atcatcatta tttttttagg ttttttcaat agtccaacaa
    44881 aatcctttat ttcatatgct ggaataattt ttttcccttt tgcctgtgtt tccacaaaat
    44941 tttttgtttt ctctcctgtt ctattataaa cagcaacagt ataaccctta tcttcaaaat
    45001 taagggcaag atttttaccc ataacctcca ttccaattat tccaatatca catttttcca
    45061 ttttcatctc cttttatttt aatttttaat ggacatttta ccttcaaaat caaatttttc
    45121 aagtttatta attcctgatg gtcttggatt tatttcaaga aaaacaggaa tgactttccc
    45181 ctctttaaat tcaagtaaga agtcaattcc cataaaattc agtaattcct ttaatttata
    45241 attgaaagca taataaacat ttaatacatc tttttttatt aaatcaatat cataacttgt
    45301 aagaattaat cttttgtatt cctctttttc cctgtaaaaa acttttaaaa aaatatcttt
    45361 aaaacgaata attttcccct ctttttcaac agaagtaatt ggtaaattat catttgctat
    45421 ttcaacaaat ccatagtcag tcataatttc gttattaaat ttccataaaa ggactctaaa
    45481 aacaatttgt ctatatcctt tttctttttc attataaaaa aaaacattcc ctctttcttc
    45541 ttttataatt actgactggc cctgtaaaat tgtaaaaatt gtttcaagaa catttttttt
    45601 attttcaaaa aaatatttac ttgtgaaaaa atatgtttct cttccttctg ttccccaatt
    45661 cggctgtata taaaaatttt caattctatt tttaataaga aattctttca ggattttttc
    45721 aatttcccct cttttttccc attccagtaa aatacattta ggaacattta ttctattttt
    45781 ttctaatttt tttatagtgt aatatttatt atctgctttc agagaagaag aaaaaggatt
    45841 caattgaaat atattatttt tttttaaaaa agcagaaatt tttttatgaa cttttatttc
    45901 ttctttagca ataagactta aaactaaaac tttttcaaat ttaggagaga attttaaaag
    45961 tactttttct acaataaaat tattgctcat ttttaaaatc tctctttcaa aattttcttt
    46021 tgaatcccat ttaattccat aaaactcaat atttcccttt cccttaagac attcaatatt
    46081 aaaaaataag ataattttct ttatatattc ttcatcagat tgataaatta ttcctatctt
    46141 tttcactttc ttattttgac tctaatcttt ctttataaat tttcgctctt tctcctaatg
    46201 acttaaatat tataaagacc ccctcccctt ttctttctgg cggcaattgg tcattatgtg
    46261 gtctccataa acctgctcct ggatttggtt caattttata aacaacaagg gatggtattt
    46321 cattgcctct tttgtcatat aattttcttt tttctccttt ttcattaaat agaggaattg
    46381 ataaaacctt tttattttta tcaactcttt ttatctcttt catattacta tcaaatattt
    46441 ttatcttagt tggattacct ttttcatcat atagaggtat aagtttttct tctttttcat
    46501 caaaaatata ttttaaatta tcatcttcat ctataagaaa aacaggcata taatccccca
    46561 tttcataagg aatatcattt attttaaaac cggttaaatc agagaaaatt tcatcatact
    46621 ttacttttga cataaattct ggataaattt ttaaatattc atttaaaaca tatttccaat
    46681 tcttttttaa atattttctt tgggattcca tacttgaaaa tgctgctttt gttatttgta
    46741 tcctcatgtc gtttctatat tttggattaa taatatcatc agtatattca aaaagtagac
    46801 cgccctgtcc aacattggca ataccttgag tacttataac agttatatga tgcgaaattt
    46861 tatagtcact tttttcaaaa acaagaatct gcctgaaata tgaatacaat ggtgtctgtg
    46921 gttctctatc aagtaataca ggaaccccaa ttcttgcaaa tctttcaaca agtttttcta
    46981 aaaattcttt tgaatataat tgccttactc ttgattcaag tacctgctga attacaacat
    47041 tatctgtttt agaaatttca aaagttaatt ctaccaattt atctatattt tcttcaatat
    47101 atttcccatt ttcttttact ggaagtataa gtgattttct tcctcctgat tctttttctg
    47161 gttttacaat caatgcatca tattttcttg ccaatttgta tattttttct ttaattttat
    47221 tctttgactt acctccatac ttttcagtat cttttatata ccagttttcc tgctcaggtg
    47281 ttttaacccc agaacctttt aacattttgg gaagttcaca tttaaggtga gttgctgctt
    47341 cccatattaa tggctggatt attggtattc tgtaagtatc tatttcagac cttaatggat
    47401 gagtaaaatg gaaaaaaatt ccatgaagtg taattacccc tttaaatgtt ccaaagacca
    47461 gtccattttc attttcacca aattttttat atttctctct gagttttttt atgtcatttt
    47521 ggtttggata gaacatacct tctcttattg taagaacttt tttatcttct tttaattcag
    47581 gaataggaaa tgaagttaag aaaagattta tattggcatt tgttgaaagg attggtcctt
    47641 gttgtaaagc aacaggaacc gttgtattgc catagggata tattatccca agattaactc
    47701 tttcagatac agataagtca gcagggtcaa tctgcataat tatttctggg tatccaaaac
    47761 cctcatattt taaaacaaca ctatctctta gacgaacata atgaaaccaa aaatcctcgt
    47821 ctttattctc ccctcttatt ccaatcccat aaatattttt atcaaaattt ccaagatata
    47881 aatcagtata atcagaagaa aatttcttca tagatgtttt ttcagatata agagacctta
    47941 aatatgtata tagataccta tgatattgac aggcaagttg aaatttctta tctcttggaa
    48001 ttttttcaaa agttgaggac ctttcttttg aataatggta agggagagaa tcaaaaatat
    48061 gattatttac tgttaaatca aaaaatttag atatttcagt attaaattcc tcatctgaat
    48121 tgagattaat tgttttttca attaaaagtg aaatatcaat aagttcaagt ccagcacaga
    48181 tttcattgta aaattcataa tttcctgtct ttacatcagc ggaactggca agatttgtaa
    48241 caagagataa atagttttta tcagttatca cattttctga aaataaatta tttatatcct
    48301 gtaataaatt tttcccatta aaagatacaa tctctttatt aagaaattca agatataatt
    48361 ttgaaatccg cagatatttt ccgaaatcat agggagaaat agtatttgtc ccttttccta
    48421 taatttcttt cgcctttcca tatataagag aaagattttt aggataattt tctattacct
    48481 tttctacatc aaggaaacct cttttctttt ctcttatgca ttttactctt tcaatttctt
    48541 tttttctttc ttcccacctt gcaagtccct caactttttc ttcaactgct tttagagaac
    48601 cagcaagtcc tgtacttatc tctctatact tatattgttt aggtggatga taaatagacc
    48661 ttaaaaagtt gttaacaata aaatctattt ctttttgtcc ataactttct ttacctgaaa
    48721 caccaaataa tttttttaat tttattctat atttttctct taattctgaa atttcttctt
    48781 tttctaattt cccatttata atataacttc ctccataaat tatccatttc ccaaaatcaa
    48841 attcatttag ttttatacct tttgaaattt ttgcaaaagt atcagttata tatctatctc
    48901 tcaaattcca tacgcctaaa ttttttgcat ctctttcctc tcttaaaaca tcgtcaaatt
    48961 ttctttcaat aagaatattc ttttttattc tatttaaaac ctctcctgct ttttctttgt
    49021 tctcatttaa tatagcagtt aaaaattcct cacttaaact tttctcatca tttaattggt
    49081 ttttccattt ttcaattgtt tcttttccta ttccaagaca accataaaca actttttctt
    49141 caagtgcaaa aagttctttt actttccttc tgcagttcgg ataattaaaa gcaaatgtag
    49201 gtcttcttgc cccagaagtt ccgtctgcaa aaaccaaccc actatttggc cttccttttt
    49261 tcatcaaata aagcatcttt ggtaaattat caactaattc taatagttcc gggtcaggta
    49321 tccccaggtc aatgaaatca ataccttgaa ttgcctcttc atatgaagaa aaaggtcctc
    49381 ttttgtcaat aacaagaaga tggattttcc cttcaaggtc ttttattatt ttttcttttt
    49441 ctttctctgt tctatccttt attcctatcc acctttttaa atttcctcca tatacaaggc
    49501 aatttgtcct tataatattt tcatcaattc caatatttaa taagtaattc tcaacttcat
    49561 tttttatttg ttcaagtcct gaattaggca catgatttag aatatctgag ccagtgcaac
    49621 tgccaacaat tctaatatca gcaggtggaa taaaatcttt gaacatctct ttaattattt
    49681 ctcttccttc cgggtcaata tcaagaagta tatgattatc tgcaacatta gaatatattt
    49741 caagttttct gcttcctgca ctgaacctca catcagagac aggtatcaat ttcccatctt
    49801 ttttctcccc ttctttataa tttgctgtca taacgcctat caattcactg tcatcaacta
    49861 aatatggatt tttcttcctt gcctgatgta taagttgata aataagttta acttttctaa
    49921 aaggacctgt tctatttatt tcttctaaac cctgtgcttt tttgttaacc cagtaagtaa
    49981 atttatattc aagttctttt tctttatttt caataagccc cattttccca agtatctttg
    50041 caatttgtgg taatcttgaa agacagatgg gctttccaat tattccaaaa tattcaggat
    50101 attttttgaa aagataagaa gttgcttttt cttcccattc taattcagaa gtaatttctg
    50161 ctcgccaacc aagaacttct cgcaaatctt ttattatatg ttcctgaatt ttttctggta
    50221 ttccaaatga agataatatc tgagaagata tttctctatc aacacatgct ataattattg
    50281 cataaagaag actgaattcc ttaggaacac aaaaacctcc atatgtttca cctgctggta
    50341 agacctttct atcacctctt gcattccatt gatttctgaa aaattcaaaa tcgcccattc
    50401 ccagtgtatc aagatttata ctgcctgcta atgaaagata atagacacca cctcttgaaa
    50461 agaaattttc ccctactttt aaaaattcag ttactcttgt agaaggaaca acagttggac
    50521 ctgcaatatt ataaaggcga tataattctt tcccagaatt tgcagaaata tcatctatac
    50581 cacctaccca tttaggaata tctcttactg aaaaaacttc ttttgctcca aggtccactc
    50641 tgcttggagt ataaagtaag ttatatcttt taaaagggcc ttctgcttca tatctaaaaa
    50701 gtggatttga aagttcactt attaaatttt cttcaacaat aacttctttt tgagcattaa
    50761 tagttgaata tgttggattg agataatttt taagaaactc ttttgtttct ttttctagat
    50821 tgaaatttct aatcatatct tctcttgttt tctttaaaat tttcagattc tcttcttcct
    50881 ttatatcaat taaatttata ttttccagac attttaaaac gcattctggt tcttttactt
    50941 taaaatcttt tctatttaaa ttttcaccct ctttttgcaa cagaattatt atttttgata
    51001 cttcttttcc tatttctgga taagaaaaaa gacattttaa tattccctgt tctccttctc
    51061 ctaacttgta aatttcccct tcaagattgt tttctttaat tattttttca cctattttta
    51121 ttatattttg tctccatatt tccatttttc cttttacttt ttcttcaagt ttgtgtcttc
    51181 ttataatatt gaataaaagc atactttcct caagccagtt ttttacatta agttcgcttt
    51241 cacccggtcc aagagtagtt aaaatatgaa tagaaggaag attccttttt ggataatcta
    51301 caagcgataa atatttctca tggagttcca gaatacaatg ttcttcaagg aatttttcaa
    51361 gttgattttt taatgattta atgcctttat aatttttcaa aaattctttt accagattac
    51421 ctttttccac ttcatttata gcaagtgtta atccttcaaa tcttagcagt ttctctcttt
    51481 cacatatttc agatatctgc ctaatcatat tctcatatga tttttcaatg agtgcagaaa
    51541 tataggcaat ttccttcttt ttatttcttg ttctttcaat taattgtttt tcatccttaa
    51601 aatttatctt taaaattttg gagagaatat ttcttgcaag aaaaacattt tctgtaagca
    51661 taacctcttc tatatttaat gcccagtttt cagcatttgt tctgtacatt tcctcaagaa
    51721 tatttctctg aacccatgca gtgaacttat aagaaccatt ttcaggtatt atttgatatg
    51781 ttgcatatgc aggaagtgct tctatccagt catcacaaat tctaagccag tccttcatat
    51841 caggtgttac ataaatgttt tcccccccat ttgcaagtaa attttcaatt tcagggtcct
    51901 ccccaaaaaa agggtcaagt aattcattta aaagtcccat atatattgca tctattggaa
    51961 aaaccctctg gaaaaatatc atataaatag gtgttcttga tggaaatttg tttaattcat
    52021 tttcaaattt ttttatttca actctgatat tgtcaaaaac ttttctatcc tgcatcataa
    52081 attctgcatt ctcatcaaat ttctggacaa gttgaaattc ataatttctt aatgtatctt
    52141 ttaagttttt caatccacat tttatctgtg caatagagac atcattttct cctgttaaat
    52201 tatcaagaaa ttgtatatca ctttctattg gtaaaaaact aatttttgac tttgaaatta
    52261 ttcttcttat tttttctaaa aggagagtta ttacatttga agagagcaat tttgaatttg
    52321 ctaaaagtaa agcatcaata taaaaagcac gactgaatct tccacataca attcttcttc
    52381 ccgattcttt tctatctaaa gcaattttct cacaacattc tctgtcttgt ttttcatcat
    52441 atgtttctct tattgcttca taaatctggc ttcttaaatt ttcaaggtct tttactgtct
    52501 ctataacata tttaactttt tctctattat aaggcaaacc aaagtttttt cttgacaaaa
    52561 gtagcgcaca caattttttt ctctttatta catctttttc tttttcccct tcctcaagaa
    52621 aatttatata ccattgccca tttttttcct tactgaatat ttttattatt ttattccttt
    52681 gagatggaga aaatttttca taaattgaaa tgtaagataa aagaacttcc tttttccctt
    52741 ctgccttcaa atttttaata aattttccta ttttcatttt atataaattc tgcaccgctt
    52801 gaagaattac aaataaaaga tgattctggt aagtttcttt tatggtaata ctttttattt
    52861 agaatttcaa gtgttttttc tgcttctttt tcttcaacta aaactaaaac acatccccca
    52921 agccctgctc ctgttaattt tgctcctttt actccattga tttttttagt agtatctaca
    52981 ataaaatcta attctggagt gctacatcta tatccacctg gctggttata gatttttgcc
    53041 tcttcttttc ctttttttaa atcagatatt aactttaaaa gatacttatc tgttactcta
    53101 taatcccatt tttctatttt attattgtct aaatgtttta caactctatc accgtcatgt
    53161 gaaataaaca ttaacttccc aaatttttct atttcattgt tttttaaaaa atattcacat
    53221 atccttgctc tttcacattc acttattcca tacatcaaaa tatctcttaa tctataacct
    53281 ttttcaggag gtatatgcgt ctcaaatagt actccaagat tttcttcagg caataattta
    53341 caaatttctt ctcttttaga aatttcagga attgaaagca aaattttata tacattttcc
    53401 tcacttttga gtatttctgg atttacatct cttaaatgca ccaatttgtt ctttaattca
    53461 ggaaaatttt tctttattaa cttaaagcct atttcataag atgctattct ttcattaaat
    53521 atatttttag catctgtaga tttttttgct tctattagtg agttacaaca tattacttta
    53581 taattttcag gaaagggtag atatttactt gtaaaaggga aaaatcttaa atggactatc
    53641 tgcccctttt taccaaaaat cattgctgca tggtctccac ttccacctct tgtacctaca
    53701 taccattctg cttctccaca catttcaaca agtttttctt tttcaatatc tatattatta
    53761 attttacagg cacaaagcat tgttgccaca actaaagcag aagatgaact taaacctgcc
    53821 ccgataggaa tttcaccata atagaaaaga ttaaaccctt ttaacggaat ttcaggaaat
    53881 ttattttgaa gatataaaat aggtgccttt acataattta tccaatcacc aggagtaatt
    53941 ttaattttcc ttataaaatc aatccagtta atctttttgg ctggtaattc attttttata
    54001 tcaaattctc ttggtggata tttttcgctt tctaaattat aggaaattac tttactatca
    54061 tttctctttc ctgctacaca aaacagttct ttgtcaatag ttaagtaatt tgtataaccc
    54121 cctctatggt caatatgcgt acctaaaagg ttaattcttg caggaacttt cagaatatca
    54181 atatcctcat cattaaactt cttgatgtat gacgcaataa gtttctttaa gcattcatta
    54241 ttttcaatcc cgtttaactt ttttgatgct atcatttaga cacctgaaaa agcattaaaa
    54301 ccaccatcta ttgggacaac aattcctgtc acaaattttg atgcatttga agatagatat
    54361 atacaaatac ctatcagttc atcaggattt cccatccttc ccattggtgt atgtgaaatt
    54421 attgtttttc ctcttggtga aaggtttcca ttttcatcta aaagtaaata tctattctgc
    54481 tgagtaagaa aaaaaccagg tgcaatagca ttaactctta gatttttgtt atattcaaaa
    54541 gcaaaatgtg ctgcaagcca ttgtgtaaaa ttacttactg ctgcttttgc agcagaataa
    54601 ccaggaattt ttgttaaagg cctgaaagca ttcattgatg atatatttat aattgaacct
    54661 ccctctttat tctttaccat ttctttccca aatacctgtg ctggtaaaat tgctccacca
    54721 aaaaggttaa gaataagaac cttttcaaga gcagaaagag gtaggtcaaa aaaggataag
    54781 tcatctgatg ttgttgcctc tttcatattt ccacctgctg cattaacaag aacatcaatc
    54841 ctgccatatt tatcaattac ttttttacat gttttttcaa tttcatcttt cttcaaaaca
    54901 tcaatataca tactatctac ttcaatccct tctttcttga attcttctct tttttcctca
    54961 aatcctttaa tatcacaaat aataaccttt gctccacttt ttgaaaaacc ttttgcaatt
    55021 tctccaccaa gaatgccaca tccacctggt attacaacaa ctttgttttc tactgaaaat
    55081 aatttttcta caaatttcat tttcttctcc tttttatata actgcggggg ttgaactttt
    55141 gttttaatta ctaaaattat tttaaaccct attttttttg atatcaaatt tttttcacaa
    55201 gcaaaaaagt aacttattta ttttttcttc tgttgataat tttctattat tatatctcca
    55261 aatattggtg ataaataaag atgccttttt cttcacaact ttatcctttt ataaaattat
    55321 tttttatgta tctcaaaggt agttcctgtt gttaagtcag atacaacggt ccccggtaaa
    55381 aattcaattt ttgtttcttc ttctgaaata gctttcccta ctgaaatctc ctctactttc
    55441 caaatatttt ttatcaatcc ttcaggagtc tttccttctg ttatcatctt catcggaaac
    55501 caacaatcct tacatacttc tttgtagtca aagaattcaa cagtatcttc aattttgttg
    55561 ttcacggttc tttctattaa cactggacag aatcctatat cttgtcctaa ccaaatgtta
    55621 tagcaaatgt ctccttcgct tatttttact aaccaacatt tatgtccatc tataactttt
    55681 tgttctcttg taactttccc tttttctaaa gcatcataaa ggaaataaga attcttcaaa
    55741 aaaattgggc aatatgtcgt ctctaatttt ataccactta agatcggccc caatcccata
    55801 tcaatctcac cgaatttctt atcactttgg gaaatgctaa tttccttccg agtgccagaa
    55861 tttctttcat actttccttt atatgtaaat ttcaattctt ttccttcaaa atgtttggtt
    55921 tccataaaaa aacaatttgg agttcgaaca tactgggata ttatttccga attttctttt
    55981 tcgacatctg tcagaattgt aatttgacat ttcactttta aagcaggata actcaaattt
    56041 tcttgatttt tcaaagatgc ctttataaat tctatagttg gaatttcttc tgcttgtaat
    56101 atacttcctg ttaaaccaca aattattaaa aacattaaaa ccaattttat atttaatttt
    56161 tttaacatat tttttttctc ctcgtatatc agttcttttc aggcacagta aaagaaatcc
    56221 ctgtcctttc atcataaact tgtgtgccag gtggaaaagt taaattaaat tcttcatctt
    56281 ttatcccttg gttaactttt atattagaat ataccatttt accttctgca ctgatttttt
    56341 tggaagaagg atcataccat tttatagaaa ctcctcctgg gaaccatata tctttgtcta
    56401 tactaaattt ttcaaaatta ataggaccta cacttattat taaatcttcc tgtctgtata
    56461 tttcaatttt tttaggcaaa aaaccttctt ccacagagaa ccatatttta tatcctaaat
    56521 aggaataatc atccttgcca tatcctttga actctttaaa tccaaaactt ggaacttcta
    56581 ctacaaaaca tttactccca gcaaaatatt cagaattaga tagtattttt aatttttcat
    56641 tttggagaat ttcttccaaa gtaataccca caggccacag atgaaataga atggatgaag
    56701 gaacccaagt cggtctaatt tttagttgaa tataataagg tgaatatccc atagtaactg
    56761 atgcaatttt acctttctca aagtttatta tataagtgtt ttcaggagtg taaatataaa
    56821 taatttcatt tatatttttt gattcatcca ctgctttaag aaaaactttt tctttgccat
    56881 ctttataaaa ccattttccg ctttttattt ccttgctatt tttataagtt agttcaaatt
    56941 ctaccgatgc atcttgtata agagacaggt tatacctaat tccttgtaaa acaaattcta
    57001 attcatcttt tggctcacaa aatacaaagt tagttttggt aatacaagca ttttgtaata
    57061 caaataccaa tatactcaga aaaataattt tttttatttt gtccattttc cctcctatgt
    57121 taaaagaggg tactgccaaa attggctaat taaattcact cctcactttc tcacaaagac
    57181 aaatgaaatg tgaaaagtga gatgtgagaa gtagaaatcc cgtaagcgta acttatcgct
    57241 tcggcgtagg gatggtctgt aagcaaaact ctaaaccctt ctgtgtaaga caatccaccc
    57301 ctgccctcct cctctactaa aacattggcg gacatgtttt ttaaaggagg gagagtattt
    57361 tcccccttta gtaaaccgac taaagttgca aagtttagca cacagagttg gtgctgtaat
    57421 gtatttcaat aggcacaacg gggatgaagg aggattttga gagggatcta acaagcacaa
    57481 cggggtaggg agatttttat ttttcatctc tcttttatct attttttgac actacctggg
    57541 tcacaaccat aacatttact tttaaaaaga tgtcccggac aactaaagta attatttata
    57601 tgataccatt ctgtgaatcc ttccaaaaat gttttcacta cagtattata taccccttct
    57661 ggatattttt ttaaatactc ttcccatatt ttctttgctt gttcttgact tttgttttct
    57721 ttatacatca tccataaaaa ataacaattt tctcatttgt tcccttttat attatttctt
    57781 ttcaggcacg gtaaatatag tattagttat cctatcatat acattcattc caggttgaaa
    57841 ttcaggttca aaaattttgt cagaaatttc gttatttact tttatgttcg tatattctat
    57901 ttctacaact gcattttttt ctcctttaat atcagtatat ccaataaatt tttgtggaaa
    57961 ccatatatca cctatttttg tatatgatat tatagaaact attgttgtag tggaacctat
    58021 ttttgtttct atttttgctg ataaaaaatt ctcttctgaa atccaaaatg tatagtcgat
    58081 gtttttataa tcagaaggac ggtaaaccaa aggataacat ttttggttat taacatattc
    58141 aacctttttc aattctatct tcccggaaga aaaaattttt tttaacagat acacctggcc
    58201 aaaaatgaaa aagtacagaa gaaggaggag taacagaata tatacctaaa ttttgtattc
    58261 caacaggagt aatcagtatt tgtttatctt gtatttctac atatttttct ccatcacaat
    58321 aaattttgcc ttgtcctttt tcatatgttt tcttaatgta ctctacaact tctttgcctc
    58381 tcttatattt ccagaaacag ttatatgttt cttttttttc ttttgtgtat ggaacttttt
    58441 catagtcctc ctttttaaaa aaccctgatt ttgaaacaga gggaggatgt tcataccatt
    58501 tctgttcttg ttttattgta taattgcact ctcctgatcg aatcatctca acagcatatt
    58561 cccacccttt taaaacaaac tcttttttac tattctcttg gcaccatcct atgttattag
    58621 acaaaatata ccctatcggc actatccaca taactatttt tattattttt gacattttaa
    58681 gtgtcctttt tatcttaatt ctttttattt tggcattgtg gtgcataacc gcaagaagga
    58741 tagtctttac ttaaaataac ttatataact gcgggggttg aacttttgtt taccccccgc
    58801 agttgaattt aggttaaaag gaaaaatcag ggtgtgtaaa agaaattagt ttttttgtat
    58861 ttccacataa aacccataaa taataaattt gaaactttgg agcaactgca actggatgat
    58921 agcccttagg tattgtaata acttcatcat ttttcactac aaatatatta tcctctgttt
    58981 catcaaatat cctcataact ccaaagccac ttttaggctt tatcttaaag aaatatagtt
    59041 cttcaagttt tgtttcaact ggtggattat ctttatcgtg tttgtgtggg ggataacttg
    59101 accagtttcc agggtcattt attgtttctc caagcagtaa tctctcagaa gaagattcct
    59161 cagttaatat atctgttata tttctaaaat atacttcttc ccctaccctt ctgaacttaa
    59221 catttttttt atcaataaat aaaggagttc cttttcccgt tgctaaacaa cttaatacac
    59281 aaatctctgt cttgcctata aattcaattc tatattcaag aaagggagga agataaactg
    59341 caacaggtgg ttcatcaaaa acatttgttc ttttcatttt tccaaataat ttattttcta
    59401 aaaagaaatt gcagtttcct tcagttataa caaaaactgt ttcatttttc tttgtattat
    59461 ctttaaatat ttcaccctct gaaaatcgaa ttatcccaca atttaaaaac tctaaatcac
    59521 ttgtttcagg tgtaattatt tgttgataac cttcaatttt ttcaatttta taatgttgtg
    59581 ccaccttatc cctccttgta tttttctttt atataaaata ccatttaatc taaactattt
    59641 caattgaata atacccattt aattgataag ttgcttctat caatattctg cctttttgag
    59701 ttaaagaatt gaataaatta tttttaaaaa taaattcatg aaattctgga tgaactttta
    59761 cttttattct cttttcacct tttctaaaaa gtaaattata gatattttct tctaattcaa
    59821 taaaaatcat attaaaattt tttacatagc cagttgcttt acatatttga cactcatcat
    59881 aaaaaaactc ctttaaactt attcctgtcc tttttctact catttcaatt aatcctaagg
    59941 atgaaagaga aagcaattta actttttcat ctccaagaaa ttttttaaag ttttcaaaaa
    60001 ttttcttttt cttctcctgt ccttttatat ctataaaatc aattattata agacctgaaa
    60061 gatttcttac ccttatttgc cttggaattt ccattgctgc ttcaatatct gtattataaa
    60121 ttgtctctga ataactttta ctttcaaaac ttcctgtgtt aacatcaatt gctgttaaag
    60181 tacttccttc ttcaataagt aaataaccac ctgatggtaa ataagctttc tcattgagaa
    60241 aattcaatat ttttttgttc aaatcaaatt tttcaaatag agggatatca taattataaa
    60301 actttagttt ccttataaat tcaggacacc atttttttat atacacttta atatcattat
    60361 aaaatttttc tccttcaact attatttctt ctgtcttttc atttacgaaa tctcttatta
    60421 ttcttgtatg taacggtaac tcttgccaga gtgaatatgg agcatgaaat tttttgtcta
    60481 atttttttat tcttttccac tcattatata aatattttgc ttcgttaaca atgtatctgg
    60541 catctttgtt ttcagcatat gttcttataa taaaaccatc atttgatgga atatatttct
    60601 ttaccagtgt cattaatcta tttctttctt ttttatcttt tattttttta gaaattgttt
    60661 ttatttttga atcaggaata aaaacgagat acttacctgg tagtgtaatc cttgttgtaa
    60721 gtttcatccc ttttaattcc tttcccggtt tagtaacctg aactattata ttttctcctt
    60781 ctttatgagt ttttgtttca ggtaataact gacgaaaatt ttcttctcca aaatatactt
    60841 ctttatctcc aaaatgcaaa aaaccatttt ctctttttcc tatgttaata aaacttgcat
    60901 taagtccctt atgaattttt tctattttcc ctttatatat atttccaaca aaaatatcag
    60961 aaggaatttt atcaacaaaa aaatattcaa tctggttatt ttcaaccaat acaaccctta
    61021 taaaataaga atcacctgtt ataattactt tcatatatta tgttccttat tattcttttt
    61081 tgatttccaa ctatttctaa caacttttta tgagaaaaac catttatctt tataataatt
    61141 tcaaaaaaat tttcactttt atttatttct cccaattctt tcagtttttc taaatccaat
    61201 tcatcttttt caattttata gacagccatt aaagttatta ctcctaagga gtggttttct
    61261 aaaggaatcc agaaacaatc tttaaataaa acgccttcta caagaaattg atttatattt
    61321 tttaaaaatt cattttcatt tattttttca tcaaaataaa tatccataat ctcattctca
    61381 ccttcaatat ataccggtag gggaggacca aaacttaatt ttggatgtgg attaaaacct
    61441 tctgtatatt ttaaaggtaa attggttctt cttaaacacc tctcaatatg tcttaataaa
    61501 tttttacttg atatatactt tgaaataccc attttttcat aaataactct taacctataa
    61561 acatcctttg acataaattt cctctatatt atgaagaaca ccaccataaa cacttggttt
    61621 taaattacca aacttatttc ttactgcata aattacaagt ggatttacag tatgaataag
    61681 gggtaattgc tgctgaacga tttcctgcca tctatcgtaa atgacttttc ttttattttc
    61741 attaagttct tttgctcctt catcaaataa aacatcaatt tctttttccc actcctcaag
    61801 aatttttttc tgtcccatat tccacagatg aagttgtcca taactcatcc atacattttt
    61861 agaaaaatgt ggttctattc ctcctgtaaa accaattatt acaccttccc aatcatttgt
    61921 aacatttaat tttgtaacaa gtgtattaaa ctcaactggc agaagattaa cattcatacc
    61981 caatttttca aggtcattaa ttataacatt tccaatctgg attctttcaa aattatcact
    62041 attagttaat atagtaaatc taatctggtt attctcatta tcaaaaagtt ttgtctgttt
    62101 gtagtggaaa ccacctttta gaagatattg tcttgccttt gataggttat attcataatc
    62161 aggtaaattt ttattataat aaaatccaca tgtggaagtc attggtccta cctgtggaac
    62221 tgcaagtcca ttgaaaacat tttttataat gtttttttta tcaatagaat gtgcaattgc
    62281 ttttctgaaa tataaatttt taaaccattt aattttgtaa ttatcaattg gtgaaaaaac
    62341 attctgatta aaggagagaa aatttgaact aagtgaaggt cctatctcat aaattgtaaa
    62401 attttcttta ttttgatatg gttctaaaac agaaaaatcc tgccctctta ttgaaattat
    62461 atcaatatct cctgtcctga atttgagaat tgtcatatta gggtcaggta taataagaaa
    62521 aataattttg gggatataag gtaatctatt ccctttctca tcttttttcc aatatttctc
    62581 atttttttca agtattaccc attctcctgg tctatactct ttcattttgt aaggtcctgt
    62641 tccaataata ttatttactt tttcattaac tccccatgta cttgttatcg tcccatttaa
    62701 aacatttttt tcaagtatat gttttgggaa aatttcttgt gtcattaatt ttaagaaagg
    62761 agcaaacttt tctggaagtt ggaattcaac agtataatca tcaactttct ttacatttat
    62821 ctttttccca tcaactgtga gaacatcttt gctacttgta ggaatacttt cattataaat
    62881 caagtcatta tatgtaaaaa taacatcatc tgctgaaaat ttttctccat cattccagta
    62941 aacatcatct cttaaataaa aaatccattt tttcccactt ttatctattt gccatttata
    63001 tgctaaagat ggttttactt ccaatgttac accatccatt tcaacaagcc cttcaaatat
    63061 aaaagttgta atattagttg tgcttgtttc ttttgcaata ataggattaa atgactttgg
    63121 gtcagaggta gttgatagag ttatttgatt tatatctttc tgagtttgat aagttgaaca
    63181 acctgaaaat aaaagtaaaa caaaaataaa aaattttttc attttataaa ctcttccata
    63241 tctgccaaaa gttcatccat agatgaatac cttaaattca aatctggatt tattgctttc
    63301 tctattatct tttttaactt atctggtatt ggcaaatcat aagaaattat tggtgttttc
    63361 ccagtgtttt ttttatttgc cctaatattt attcttttta aataatcttc atcattatat
    63421 ccttcaattt gcagtttact tgtaataact tcatcaattg taacaccaaa actataaata
    63481 tctgattgaa atgtcgcatt accttcttcc tgttcgggag caacatagga gataattcca
    63541 aattttgtaa ccgttcctcc ttttggaaaa atatcttttc tccatagttt tcctggtaat
    63601 tttgcaatac caaaatcagt aattttaaca atctccaaat tctttgacac cagaatatta
    63661 tctggtttaa tatctttatg aataatatta tgttggtgaa tataacttaa tcctttacct
    63721 gctttataac aaatttttat tattgtttcg aatggaaatt tttctctttc atataatatt
    63781 tcttttagat ttttcccatc aacatattcc attatcattg cataatatct attaaatctc
    63841 ccgaaattga aaacttttac aatatttgga tggtcaagtt gcatagcaat ttccgcttct
    63901 ctttcaaatt gacttattag ttttgatttt tcaaaaattc ccgcttttaa gttgagtatt
    63961 ttaatagcaa caggattacc atagggagga tgggcaagaa acgaatatgc tttatatacc
    64021 tttgtgtatc ttcctcctcc aattacttct cctaaacaat atcctccaaa atttttatct
    64081 cgcattttct tctttttcct ttgtattaag tttcctgttt aaatttcaga gcaagtagtg
    64141 aaaaaaaacc ttctcacttt tcttgtgctt actaatcttt tttataaaat cctccttcat
    64201 ccccgttgtg tctattgaaa tacattacgg caccaactct gtgtgctaaa ctttgcaact
    64261 tcagtcggtt tgcaaagggg gaagtatgtc tccttccttt gaaaaaggaa ggttgggatg
    64321 gatttctctt ttttacactt tcaattcgct acttttcggt caacacagtt gactgagtat
    64381 ttatatccca tttaaatcag gtaagtccag agaaactaat tcaatatcat caacattttt
    64441 tatatctttt tcaaggtcaa caattgctat tttcctttca acaccatatt cagaagatgt
    64501 aaaaattgtt aaacaccttt tatcaaacat aacttctttt gccttagggt catgacttct
    64561 tattaaaaca ttctttttta atttgcccat aactttttca aaataatcct taccaagttt
    64621 aggtcttcct aaaaaactac ccagcaattc tccttttttt tcaacaaaat ctccccataa
    64681 cattataaac catttttttc ctccaacttc aatattctca atatctaata atttttcaac
    64741 atctggaatt gctgcatgaa ctccaattaa attattacct gtaacacacc acggtaattt
    64801 gagaaatatc tctttataat gattaaattc ttctggtgaa agtgcctccc agaaattaga
    64861 aggagagcac tcacaaatag gatacacctc atgatttcca gcaagcaaaa ttaaattatc
    64921 atatttttct cttttttcta acaaataatc aatattttct tttgaccttt ttcctctatc
    64981 aacataatca ccaaggaaaa caacataata gttattttta tagagaaaat tttttaaaat
    65041 aacttttgat gcaataatat cgccatgagt atcaccaaca aaaacacatt ttcctttgtc
    65101 aggtaaggta attactcttt ccatttgaat attattggct ggcaggaggg attttattct
    65161 ctgttttttc tacctgttgc tttaatatac ttttatttgt taatttgccc ggagtaacag
    65221 caagaattaa ggaagagaca ataaaaatta ttgctaaaat agttgtaatt ctatttaaaa
    65281 tagtaggcgt ctctgcccca aaaacagttt ccattcctcc accaccaaaa atattagcca
    65341 tagaagaacc tttgccactt tgtatcaaaa caaaaaacat taaaagaaga cagacaataa
    65401 tatgaaaaac taaaagaaaa gtgtacattt tttctccctt ttaatcaact caaatttttt
    65461 tataattatt tctctttccc ccttcccttc ctctccctta aagaaaaatt tcttatactt
    65521 tataattaat aattcttaaa aatttatcaa tatctaaact tgctccacca accaaaacac
    65581 cgtcaatatc tttttccttc attaaatcat ctatattatc aggacttaca ctccctccat
    65641 aaataactct tattaaatct gcaatttcct gattataatt ttctccaatc cattttctta
    65701 taaaagtatg aacttcttct gcttgttgtg gagttgcatt tttcccagtt ccaattgccc
    65761 agacaggttc atatgcgatt gtcagttgtt caatatattc aaaatcagat agacattttt
    65821 ccagttgttt tttaataata tcaaatgttt tattgctttc tctttcttct aatttttcac
    65881 caatacacag aattggtttt agtttatatt gaataacttt tttaactttt ttatttataa
    65941 tttcatctat ttcaccaaaa atatttctcc tttctgaatg tccacaaata acatactcaa
    66001 cacctatatc tttcagcatc aaaggagaaa tttctcctgt aaaagcacct ttctcttcat
    66061 aaaaaacatt ctgagcccct aaaagtaatg aggaatttct aataatatca gaaatgggat
    66121 atagagatgt aaaaggagga caaataagaa tttctctatc attataattt ccaatattct
    66181 taattaaatt cgtcacaaat tcaattgatt cagaaggtgt tttattcatt ttccaatttc
    66241 ctgctattaa tgtttttctc atttaaaaac tcctattttt ttaatacatc aattccaggg
    66301 agagtacctt tttctaaaaa ttcaagagaa gcaccaccac cggtagaaat atgagaaatc
    66361 ttatctgcaa cacctgcctt attaacagca gcaattgaat cccctcctcc tataacagaa
    66421 aatgcatttg aatttgccac tgcttttgct atttcaattg tcccttttgc aaatttatca
    66481 atttcaaata ttcccatagg tccattccag aaaattgttt ttgccctatt gacacaatca
    66541 gtatattctt caattgcctg tggtcctata tcaactccaa tccatccctg aggaatagtt
    66601 cctctttcgg taattaacac ttctgcatca ggagaaattt tatttacaat aatatggtca
    66661 agtggagtat gaaactccaa gtgcctttta tttacttctt ttataatttc ttttgcaaga
    66721 ggtattgctt cttcatcaac ttttgaacta cctatttccc agtgttttgc ctttataaat
    66781 gtataagcca taactccacc tgtaattaga gtgtcaactt tatcaatcaa attttttatc
    66841 attcctattt tatcaacaac ttttgctccc ccaataatta aaagaaaggg cttttcggga
    66901 tttgataaaa gttttgataa ggcatcaacc tcctttttaa ttagaaaacc agcagcagtg
    66961 tctttacaat actttgttat ctgataaatt gaagcatgct ctctatgaca ggcagcgaaa
    67021 gcatcattaa catatgcatc agcaagtgaa gcaagttgtt ttgcaaattc atcatttcct
    67081 tcttcttctt ctttgtgaaa tcttaaattt tctaaaagta atacatctcc tttttttaaa
    67141 ttttcaacta ctttttttac ttcatcacca acacaatcag gaacaaattt aacttcttta
    67201 tttagtaatt cacttaatct ttctgcaaca ggccttaaac tcatttcagg aacaactttt
    67261 ccttttggtc tgcccaaatg agaagatata attaaaatag caccctgtga aagaagataa
    67321 tttattgtag gtaatgaggc agttattctt gtatcatcag ttatcctgcc attttcaata
    67381 ggtacattaa aatcaactct caaaaaaact tttttaccgc tataattttg ttcttctatc
    67441 gtcttaattt ccattttacc tccttcttac tgtaaaggtt ctgccaaaat tggttaatta
    67501 aattcactcc ccactttctc acaaagacaa atgaaatgtg aaaagtgaaa atccatcccc
    67561 tcgggcatag ccctacgggc gagcccacct tcctttataa aaggaaggag tatcttcccc
    67621 ctttcacaaa ccgactaaag ttgcttacct tgtttctcag agttggtgcg ttatcgtttc
    67681 ccagatagca caacgggact tagggggatt tataattaaa atccatcccg gccttccctt
    67741 agtaagggaa ggagtatttc cctcattact tcccccttta gtaaaccgac taaagttgca
    67801 aagtttagca cacagagttg gtgccgtaat gtatttcaat aggcacaacg gggattgagg
    67861 ggaatttgaa gagggattgt tctacacagg cagtttaaaa tatagcatac gaaccatccc
    67921 tacgccgaag cgataagtta tgcttacggg atttttattt ttcatctttc tttcatctaa
    67981 gtttttgaca ctaccactgt aaacattcat ttctctacct gtatagaaaa atttttatat
    68041 ccccttcttt cttatcctaa atctccgttc ctcccctgtt ataaataggg aatctttgct
    68101 ctctttataa attccctttt tcaaccataa atttcaaaag gtcaacaact ctgcaactat
    68161 atccccattc attatcatac cagccaagaa cttttaccat atttccaatt atatatgtca
    68221 aaggtgcatc ataaattgaa gaatatgggt tcttaacaaa gtccttagaa acaagttcat
    68281 cttctgaata tttcaaaatt ccttttaaat atgtttctgc tgcttcttta aaagcagtat
    68341 ttactgtttg tatatcaact tctttttcaa gttcagcaac aaaatcaaca atagaaacag
    68401 ttggagtagg aactcttatt gcaagcccat tcatttttcc atttaatttt ggtataactt
    68461 tcccaattgc ttttgctgcc ccggttgttg taggaatcat agaaagtgcc gcagcccttg
    68521 cccttcttaa atccttatgt ggcaggtcta aaattctctg gtcatttgta tatgaatgta
    68581 ttgtagtcat aagtcctttt acaacgccaa aattatcatc aaggactttt acaagaggag
    68641 caagacaatt tgttgtacat gaagcatttg atattattgt atgattttta gggtcataca
    68701 tattttcatt tactcccata actattgtta aatcttcacc ctttgcaggg gcagtaatta
    68761 caactttttt tgcaccacca acaagatgaa atgatgcttt ttctttatct gtaaaaagtc
    68821 ctgttgattc aacaacaata tcaaccccaa tatctttcca tggcaatttt gaagggtctt
    68881 tttcagcaaa aactatatat tcttttccat caattactaa tttgttatct tttccttcaa
    68941 cagtaccagg ataaatccca taatttgagt cgtatttaaa aagatatgca agtgtctttg
    69001 catcagtaat atcgttaaaa gcaacaaaat ctatttcagg aaaactcttt tcaagagcac
    69061 ttctcaaaac cagtcttcct attctaccaa atccatttat tccaactttc accgccataa
    69121 tttcctcctt tttaatttat ttttatattt tgtttatttt aacactaaat tttttaattg
    69181 taaatacaa
//
LOCUS       DAOOVW010000003        53398 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_387_length_53398_cov_9.116829, whole genome shotgun sequence.
ACCESSION   DAOOVW010000003 DAOOVW010000000
VERSION     DAOOVW010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 53398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 53398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53398
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_387_length_53398_cov_9.116829"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..1221
                     /locus_tag="PLW95_00700"
     CDS             157..1221
                     /locus_tag="PLW95_00700"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOV21188.1"
                     /translation="MKTKNFILIPRKYYDIYYGRENVAIKSIKELHGNDIGIKIVLYS
                     PNNTVSHFLFSTGQNDFSKLKNNTITVKFIDNDQIQDAIYKIKPIFKDSKETEIYTIN
                     IKCYPSKFYASQKMTGHPHIIALYNDPILNIGTGSVDDWLVTPTEEEKKFALNKWGSL
                     VENEKTDYEKAKILAKTLMHDLWPHSGFPSDAMNVPPFEQYKRMVSGKDKGYCSNFMA
                     IFVHACNSLGIPARSCGINEIYFNRNNSEKCKKMNIQIQGGSCHGTTEIFDNGLNHWI
                     WMDLRYYALGAWIGNIGPLTLAEFQIFINQKQRREKLKLLIYNMENKSEKLLPLNKCP
                     KQEFSCFTGWDVKLNYQKMK"
     gene            1222..3672
                     /locus_tag="PLW95_00705"
     CDS             1222..3672
                     /locus_tag="PLW95_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21189.1"
                     /translation="MENIQKSEKIQVSYEDTTQRQHLCIPAIETSMSRRYLSIMQNWI
                     SFGMEYFHDWQVCPHCGHFFGGVFWYGMETSSPLFVIAAVVSSPEYNQKVTGYTKKQL
                     INVAVKALRYLCFTHDTGPQDCVRPEKGLGNATLFGTKWGEKGKGFFPESQCGVTLYK
                     IVISALILRKWIDRETWMMIAKICADYLERFVDMSPKSGVYYDTQAEENTWTSLGLTA
                     AMLFLENHKDAKKWEESAKHWMFCAAIVPDDLHDDRKIDKIDKKKSIRELCSKKVTFL
                     PDFMAENHGIVHPNYTASVILGDIINLYRLFGRKEPSHWYWHRQEVYDNLKFLSDKNG
                     IVHPVQGMDWPYLTPSGLPSVHCFAHLYLKDKEGAYFENAALNLAEKIIKGNKGRMWH
                     PDIVQYCHNIQDPMLLWEHQICNFVPLYLAHRICAKEKIMKVPSQKIIFAKYVGVRKY
                     PHSGFIFHKHKNGQTSFSWRNQIMALPVTSDGFLTIAPSFNSILGKIKVKGYTEHFKV
                     EYIHINEKEDGFTAIMRVILNDGCIKQDIMLASLPDGKFVCKEVFWALKGCTVEYIQQ
                     GYIEIMNERFPELKINCTGSRKLYTPSGVYNFPSMVKPNSKYDKKVELKSPPWLNLDD
                     RMGIIIASEGHQTLYHNRHFFKPYHAVTDELFLNMHNKKNFYKKNSIIADILFMFIPG
                     QNHKDTPKQLFITPLSCSEGIWGALTDNNLIVGNLDVCSGSYSIEFKRQKTISVFPGI
                     TKIENGKVSYEVDIIKGQSLFMESLFSIETNGFFIVSALPGGLVYITNNGRAKEILSL
                     DGEKISVDAGKTILQKKR"
     gene            3726..4382
                     /gene="hypB"
                     /locus_tag="PLW95_00710"
     CDS             3726..4382
                     /gene="hypB"
                     /locus_tag="PLW95_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HOV21190.1"
                     /translation="MGIKIIKIEESVFAENDKIAQENYKLLEKKGIISFNFMGSPGSG
                     KTTIIEEMIKKLKDKYKIGVIEGDIAGCLDSQRVEKLGIPVVQINTGGACHLDANMVK
                     KGIEKLPLENLNIIFIENVGNLVCPAEFKLGNKINIVVTSTTEGSDKPVKYPLMFNIS
                     EICLLNKIDIEDNYFEKDTFLKNLKKVNPHIVVFEISARRGDNFEKWINFFENKLLNN
                     "
     gene            4544..5941
                     /locus_tag="PLW95_00715"
     CDS             4544..5941
                     /locus_tag="PLW95_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21191.1"
                     /translation="MKKLFCAVVLSVLFVSYGSCDLKEKGVSLTIYNQNFALVSDVRE
                     GKIEKGESILKFTDIASLIDPTSVSFSSLNYPGLLSVIEQNFEYDLLNPDKLLNKYIE
                     KNIQVITKDGQLYSGQLLSFNSGQLIIKSKEENVFMLNRENIRDITFVEIPEGLILKP
                     TLVLEIRNNKEVSHILQLKYITDGINWKADYVAELSPDDKKMDLTGWVTIDNRSGVTY
                     KDANLKLVAGDVRKISQYREYAMPAGKVLAAQAAPQFVESPFFEYHLYTLTRQTTIKN
                     NQTKQITFLSSANVPVRKKFIYDGAANRYYHYDSWRSVPYNDKVEIHIVFENKKENNL
                     GLPIPKGRVRFYKKDTENTTQFIGEDNIDHTPENEKVELIIGSAFDIKGERIILEHNK
                     ISSNIYSDTYQIKLRNHKKEKVEIEVIEHLYGDWEITKGSQNYEKVDANTIKFLVEVL
                     PEKEFVINYTSVSKF"
     gene            complement(6366..7073)
                     /locus_tag="PLW95_00720"
     CDS             complement(6366..7073)
                     /locus_tag="PLW95_00720"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HOV21192.1"
                     /translation="MGNLLGSDLQKKICVVLTGNEENSDIEKLKDIALVECRIDEFLK
                     RKTIDELPEWILKIKEKLNTKIIATIRWYKESGQNPYYIKDKERLEIFKKIITYVDFI
                     DVELKSRILKEVTEIVESQNKKVIVSYHNFKKTPNEKKLKEIVRKGNKVGDIVKIATL
                     IKTEKNLFDLIKITYTYSKKIPLIIIPMNENPFVRFIPIFFGSLFTYVSLNTKVAPGQ
                     ISYFEYSNFISKPVPDK"
     gene            complement(7142..7378)
                     /locus_tag="PLW95_00725"
     CDS             complement(7142..7378)
                     /locus_tag="PLW95_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21193.1"
                     /translation="MAERLRGILKGIRGDFDTSPFNKGGKRGISNFPLTPTLSPVGRG
                     EERGDVFSSPLILSDKIYGGVRKLGGGGILREFY"
     gene            complement(7580..8245)
                     /locus_tag="PLW95_00730"
     CDS             complement(7580..8245)
                     /locus_tag="PLW95_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase
                     domain-containing protein"
                     /protein_id="HOV21194.1"
                     /translation="MIDLHTHTILSDGCLLPSELARRAEEKGYKVIAFTDHIDFSNVE
                     MVVSSLVRVCDRINRNYKIKTIPGVEITHVNPIDIPVLVQIAKNIGAKLIVVHGETIV
                     EPVSKGTNRKGIESDIDILGHPGILSEEDAILAAEKKIYIEISARKGHCLSNGHIVRL
                     WYEYGFPIILDTDTHSPENLIDDEFAKKIIISAGIKVSDVNTVLQNSYILAKSKLDIG
                     FEI"
     gene            complement(8238..8615)
                     /locus_tag="PLW95_00735"
     CDS             complement(8238..8615)
                     /locus_tag="PLW95_00735"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOV21195.1"
                     /translation="MENGELGSVRIDNEVLGTIAGIAAKEVPGVYKIITSLVGGIAQL
                     IRKTHDAGIKIIVRENEVSFELGIIIEYGVDIPEITYQIQKKIKEEVERITGLKVIKV
                     DVVVHGVHYSRGKQKTEKEGEND"
     gene            complement(8682..10037)
                     /gene="accC"
                     /locus_tag="PLW95_00740"
     CDS             complement(8682..10037)
                     /gene="accC"
                     /locus_tag="PLW95_00740"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018343087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOV21196.1"
                     /translation="MIKKVFVANRGEIALRVLRSCKELELKTVIGYSEVDKNSYPVKF
                     ADEKICIGGSSPSISYLNIPSIISAIEITKADAVHPGYGFLSENIQFVDICNATGITF
                     IGPTAENIKTMGDKPLARKIAKKCRIPIIPGYEEFNYDKALHHAKKIGFPVIIKASAG
                     GGGRGMRIVSSADEFENLWNTCYEESKISFGEGGLYIEKFLERPRHIEIQLLADKKGN
                     IYLFPERDCSIQRRHQKLIEESPSPVIGIKLRRKLQKYAYKLAKNIKYLNAGTIEFLV
                     DKDKKPYFIEMNTRIQVEHPITELVSGIDLVKSQILIASGEKLSFKQRDIGIQAHSIE
                     CRINAEDPENNFLPSPGKIEKLFLPSGPGVRVDTHIFEGYIVPPYYDSLLMKLITYGR
                     DRNEAIRRMKRALEETVIEGVKTTIPFYKEIMDNPVFLSGRYYVGYLENYIAKKVINK
                     S"
     gene            complement(10034..10459)
                     /gene="accB"
                     /locus_tag="PLW95_00745"
     CDS             complement(10034..10459)
                     /gene="accB"
                     /locus_tag="PLW95_00745"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HOV21197.1"
                     /translation="MKTDEIKVYAEFMKKYDLDYLEIKKGDFHLILGKKSEWETKEEI
                     NPSDILKKTEEQTQKIENIVYVKSPLVGTFYRSPSPESPPFVEVGKEVKNGDVLCILE
                     AMKVMNEIKADVDGTIKEILVENGKPVEFGQVLFVIEIK"
     gene            complement(10468..11022)
                     /gene="efp"
                     /locus_tag="PLW95_00750"
     CDS             complement(10468..11022)
                     /gene="efp"
                     /locus_tag="PLW95_00750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOV21198.1"
                     /translation="MKVIDLREGSLFKYEDNIYSVLSYSHLTQSRGRGKVFIKVRDMK
                     TNKTVELTFRSDFEVGDVFVDEIPLTYLYREGNVFYFMDSNTYEQFPVAKEIVGEKEK
                     FLIENVQTYGAFLDGEIIDIILPTTVDLKVIEAEPGYKGDTVQGGRKKVKVETGLIVQ
                     TPLFIEIGDTIKVDTRTGEYITRV"
     gene            complement(11619..13217)
                     /locus_tag="PLW95_00755"
     CDS             complement(11619..13217)
                     /locus_tag="PLW95_00755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HOV21199.1"
                     /translation="MENKITQLLSKVKNPASYLGKEINVYIKKWEKEKVKIALSYPDI
                     YSIGMSSLGFRILYGMLNERDDCICERFFSPLEDMEEILKKEDIPLFTLETKTPVMDF
                     DIVGFSLSSELNYTNVLNLLDISKIPIFSKERGNNEPIIIAGGNSVFNPFPIIPFIDV
                     FVIGEGEEAMEKIVETYRELRGEKREKILKCLSEIEGVFVPMSPKNNIKKATIFDFEN
                     SYFPKKYLVPLTDIIHDRISLEIMRGCYRNCKFCQAGKCWKPVRKRSPEKVLEIAKET
                     FEKTGYEEISLLSFSAGDHPEIEKIVSLLVDEFKKRNVSISFPSLRLDTFTFQLAGKI
                     KEIKKSSLTFAPETSENLRNKIGKEMGNTKLIELCQEARESGWRHIKLYFMIGLPDET
                     EKDISEIVNLINDISKIIKVNVSFNTFIPKPHTEFEKEKFISLDGYEQKKNFITESHK
                     KNRYVKVDFHPYNMSCVECFLARGDEKISKVIYNVWKKGGKMENWNEFFDFEKWTESF
                     KETGIDMKKYTNYIESTPWKIISL"
     gene            complement(13217..14302)
                     /gene="rodA"
                     /locus_tag="PLW95_00760"
     CDS             complement(13217..14302)
                     /gene="rodA"
                     /locus_tag="PLW95_00760"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOV21200.1"
                     /translation="MGLFRYFKERDDSTFIVILTYILVIIGLLLLYSASKGIIFKSSF
                     FVKQCIWVILGTIIIIFLNGVNYRELKRFAPILYFIILFLLLIVLATGEKGPSRWINL
                     GWLYFQPSEFAKFVLIVALSAYLENRNIRKFSVFFTSLLIVFIPFILVLLQPNLGTAF
                     IFIFISLGILYQADMSRKQFLSLFFIVLLLSPILWFSMKGYQKERILTFINPGRDPIG
                     AGYNLLQSKITIGSGGLIGKGFLKGTQTKLAFLPEYHTDFIFCVLAEEFGFIGVLIFL
                     FLYYLFFKKIIEIISFTTDEFGKLLATGILTLFVAQFTINIGMTMGLFPIVGIPLPFI
                     SYGGSSLITCLICIIFLKNIQDSWSVF"
     gene            complement(14289..16061)
                     /gene="mrdA"
                     /locus_tag="PLW95_00765"
     CDS             complement(14289..16061)
                     /gene="mrdA"
                     /locus_tag="PLW95_00765"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOV21201.1"
                     /translation="MEKERLFYNLVKFLVIILGVWCFFLQVVRYGYYSQLSAKNCIRT
                     INIGTPRGVIYDRNGKILAKDIPTLNLVFIPFDIKNIEKEAEILSNIISFNKYFLIEK
                     FKKKYTNPYDRIVIKRNLSIEEVSLIEENIDNLPGIFPQESIGRQYPLEDQTCHVVGY
                     LGEISKEELDALKDKGYKIGDTIGKDGVERYYDTFLRGSPSGIQVEVDALGHYRKVLK
                     KQAGKTGNSLILTIDSVIQRSAYKQLGNRSGCVVAMDPRNGEILALVSRPGFKPGEVE
                     KYFNLASHPLVNRVIKGQYPPGSIFKIITEISALETGEIQEYDRIECRGAMEIRDRVF
                     HCWKDEGHGLIDINLALPFSCNIFFGTCGMKVGVEKMLEYASKFGLGEKTGIDLPGEK
                     SGHLPLKKETDPLNLAIGQGPILTTPIQLLSLISTVANGGNIWEPFIVKKIVSPDGNV
                     LKECSPFINKTVFISSDTLEILKKGLRNVVLFGTGHLANVEGFEVSGKTGTAQRAGGE
                     SDLPTYGGFVCYAPSSNPQIAIVVFLDHASSAEAALIAGKILKEVFIPEKGRIEENGE
                     TNEETQEENKNPIEKSEEIENGII"
     gene            complement(16031..16513)
                     /locus_tag="PLW95_00770"
     CDS             complement(16031..16513)
                     /locus_tag="PLW95_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21202.1"
                     /translation="MEKKGIIRIFEVFLVVVGIQIIFSIYFSPNVVPCFLLIFLLNLV
                     WEGNIEISLFLSFVSGIIYDTINKSIPGFSSIIFLIIVYINSFYKVRSFLIRNILVFI
                     FSLMYFIILPMKIGEGFMWNKQVILKYSLIFAILNVIVEYFVYLGKILKWKKKDFFTI
                     "
     gene            complement(16491..17162)
                     /gene="mreC"
                     /locus_tag="PLW95_00775"
     CDS             complement(16491..17162)
                     /gene="mreC"
                     /locus_tag="PLW95_00775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOV21203.1"
                     /translation="MIWRFRKEILFFILIYFSFLINKLYFSRNFSKNLVKKNIINYSE
                     IINENERLKKILKLNEEGKLNEFIVGRIINFIPSLFPGELVVDKGQKENVETGMVVIT
                     DDLNLVGKVERVEENVSYVSTIFNSKTKISSVLFSTGEVGIIEGGTTPFVLLKYISSD
                     SNIKIGDEVLTSGYSKSFPAGIKIGKVVKIEKQTNSLFLKIYVKPYCMYSYIKEVLIG
                     KERHN"
     gene            complement(17190..18218)
                     /locus_tag="PLW95_00780"
     CDS             complement(17190..18218)
                     /locus_tag="PLW95_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOV21204.1"
                     /translation="MVKFLTFFSNDIGIDLGTANTCVYVRGKGVVLMEPSIVAMNTTT
                     KEFVAVGGEAKKMLGKTPANIVAMKPMKDGVIADFDGCEAMLRYFISKVKTPKHKFVL
                     PPRIVISVPSGITSVERRAVKETALKAGARMVRIVEEPMAAAIGVGLPVEEPCGSLII
                     DIGGGTTEMAVISLGGIVITKSLRIAGNEMDEAIIEYLKKKYNLLIGERTAEEIKIQI
                     GSAYKLNEELEMDVHGRSLVEGLPKQIKINSEEIREALKDTLSLIVNAVRDVLEETPP
                     ELSSDLIKEGGVVIAGGGALLKGIDKLISQETKLPVIVADDPLTAVVRGTGKMLEEEK
                     YFKHFPQE"
     gene            complement(18230..18493)
                     /locus_tag="PLW95_00785"
     CDS             complement(18230..18493)
                     /locus_tag="PLW95_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor-like protein"
                     /protein_id="HOV21205.1"
                     /translation="MKEMKIGEITHYFNKIGVAAIKITNDQLKRGDEIHIKGHTTDFS
                     QKVNSIQIEHQEVEVAKVGDEIGIKTDEIVRDGDEVFKIIEEM"
     gene            complement(18453..19892)
                     /locus_tag="PLW95_00790"
     CDS             complement(18453..19892)
                     /locus_tag="PLW95_00790"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV21206.1"
                     /translation="MNEKILIISDKENLKKLCRDLLEKRNFIFEETNNGFVAIDKIKN
                     NEFTLILLDLQISEIDGFQFIKNVREFNNLIPIVVIDEEIKNAVKAINSGANDLLIYP
                     FDINQFFKTIKKNIEIGSLLKEVSRLKIFETLFELNKNIGFLTDIDTFLENISDILLK
                     TFSPERISIYLIDKGSSNFILRKHKYIADKTNKQKMSYSKEEINKIFGSEIVKLDEQT
                     KTIRAFVRIKGKEKEVGLLDIEISKNIGIFEKNEVKFLELFSMQIGIGIENSILLETI
                     RGSYFLNTIECLIKSLEVKDGYTKQHSEMVAYYATLLGKRLELSDEEIETLKISSFLH
                     DLGKLGVKNGILSKEEPLTPEEKYFIKKHPLITVNILRPLNVDKEKVEACLYHHERVD
                     GRGYPEGLSGEKIPLYAKILAIADAYSAMRADRPYRKALSKEEAISELLANAGTQFDR
                     ELVNVFIDILNKMEVNNEGDENRGDNTLF"
     gene            20020..20736
                     /gene="lptB"
                     /locus_tag="PLW95_00795"
     CDS             20020..20736
                     /gene="lptB"
                     /locus_tag="PLW95_00795"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013681476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOV21207.1"
                     /translation="MLETKNLKKSFGRKVAVRDISINVGKGEIVGLLGPNGAGKTTTF
                     EMVLGFLKPDKGNIFVDGIDITNFSVWKRSQLGISYLPQEISIFSKLTVEENFYIILE
                     SYYKDKKKMKQVVEEYLKKIGLYDMREQPAGTLSGGEKRRLEIGRSLSLNPKFFLLDE
                     PFSGIDPKTVSELQDIVVKLKNSNIGILLTDHNVREALTITDRAYIIYQGKILIEGKP
                     EEILKHPEARGVYFGEKFQI"
     gene            20733..21437
                     /locus_tag="PLW95_00800"
     CDS             20733..21437
                     /locus_tag="PLW95_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012674860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOV21208.1"
                     /translation="MRKNLTILPVHVGIIMDGNGRWANKKNLPRIFGHKEGIKAVRRV
                     VKAAKKFEIKYLTLYSFSTENWKRPQEEVSFLLKLIETRLIKEGNNLNKNNVKVDFIG
                     KKEKLPKKLIEIMDRVRNLTKKNTGLTLIFAINYGGRQEILDAIEQIIKHGNIKKVEE
                     ENFRNFLYLPSLPDPDLIVRTAGEKRISNFLLWQSAYSELYFTDVLWPDFDEEDFYKC
                     LVSYQRRKRKFGGLVK"
     gene            21434..22537
                     /gene="dxr"
                     /locus_tag="PLW95_00805"
     CDS             21434..22537
                     /gene="dxr"
                     /locus_tag="PLW95_00805"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOV21209.1"
                     /translation="MKRVVIFGSTGTIGINTLEVINRQKNKFKVVALACKENKKILSQ
                     QIEQFLPEFVYIEERDTSFERKFKNIKFFYKGEMSEISKVDSDIFIFAIPGIDSLDTF
                     VECLKRRKKIGLATKEILVVAGSIIKNLLKKYKSEILPIDSEHNAIFQIIEKENKKDI
                     KKIYLTASGGPFYKTKIKSPSLKQVLSHPIWKMGKKITIDSATMMNKCFEIIEAHHLF
                     SLPFEKIGVLFHPEAIVHGLVEFIDGTIKAIMYKPDMKIPINYVLNYPEREGESLEIL
                     PLDKMKKLTFRAPSGKEKFIKFAKESIKINGSYPVVLNTANEVSVRYFLENKIKFQDI
                     LGTIEKIIKKQKIQKNITINDIYRITEEIKNSY"
     gene            22542..24167
                     /locus_tag="PLW95_00810"
     CDS             22542..24167
                     /locus_tag="PLW95_00810"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HOV21210.1"
                     /translation="MGKIYFPQSKIGKKGFPIETELTILDIAQRNNIQLSAECGGQGK
                     CGKCVVRIEKGKRNLSPLNQIEEKFFLSDNERLACQTKIIKDNGDIVVYIKDSGRYEI
                     LKFGMERKIPICPRFYKKNENVYKEGEIIDVYRGNIFGLAIDVGTTTVVFDIVNLENG
                     NIIATVAKTNPQISFGNDVISRIEYVSIDKNQKRYLNDEEKRERVKKLQRVIIEAINN
                     SIDEISGKYGENFSEKIYSVVVSGNPTMRNLFFGIDVSSLGVSPYEPVHKEEIIVMPE
                     EIGLKINKNGDVYGIPLIGGHVGGDIVADILSSEIYKNEEISIIVDIGTNGEVVIGNK
                     YKIISTSAAAGGAFEGGNISFGVGAIEGAVKKIEIIDGRVFYSTIGNKYPIGICGSGF
                     IDLLAELLKYNILSKNAKLKQDFYITGEIKITQADMYQLITSKSAIKTAQDILMKCYP
                     VEKENLGKIYISGGFGNFINVKNAMKIGLIPEIEEEKVIKIGNGSLEGAREILLCEER
                     KKTAEEIARSVVHIKTNEVEKNFDYILAENMYF"
     gene            24180..25295
                     /locus_tag="PLW95_00815"
     CDS             24180..25295
                     /locus_tag="PLW95_00815"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOV21211.1"
                     /translation="MNSRERILNVFEGRKVDRIPISLYEFDGNYDSWIYNYPEYVEIL
                     NYAKGKTDKMYFWFPIINSPSIFYGQIDLNDIKTEEWEENDQFHTKTTIRTPLGEVYT
                     HSRQDKGIHTSWTIENLCKNINDAKKILSLPYIPMKVDVSSFFEKDKKLGEEGILMGD
                     IPDALCLVVSLFGFSRFLMFYIDNPDIIFKLINFFQERIYNYLKELLENGAITVYRIC
                     GPEYATPPYLSPNEFDKLVTPYDKQLFQLLHKYGAKGRLHSHGKIKNVLKAFLEIEID
                     ATDPIEPLPDGDITLKEARKILGEKIVLIGNIEERLLEIGSKKDIEDAVKKAINEVGN
                     TPFILCPTAMPLTTPLDKKIKENIIHYIDCGGKYGKL"
     gene            25365..25973
                     /locus_tag="PLW95_00820"
     CDS             25365..25973
                     /locus_tag="PLW95_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOV21212.1"
                     /translation="MIENIKWLGHSSVLIEKEGKYIYVDPWKIKKDEPKGDLVLITHP
                     HYDHCSPEDIDKIRKDQTIIIGPSDAVETINGNTRKIKPYEEIELPYVKIKGVPAYNI
                     NKNFHPKSNNWVGYIIKFPDSSIYITGDTDFIPEMKGVKVDIVLLPIGGTYTMDAEQA
                     AEAINNMNVKVAIPIHYGNIVGSKKDAEKLTSLVKNAEVKIL"
     gene            complement(25970..27025)
                     /locus_tag="PLW95_00825"
     CDS             complement(25970..27025)
                     /locus_tag="PLW95_00825"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOV21213.1"
                     /translation="MKNGKYCVGFIGFGGIASSTHFPGWKKLSDVEVIGIADVSEKAR
                     KQAEKEGIKYIFDNYKKLLEIQEIDIVDVCTPNKLHYPAVIDSLKAGKHVICEKPLAT
                     SVKEVEGMIEQAKKSNKKLMAAQHQRFRKESVLTKKLIDDGFFGEIYFAFANALRRRG
                     IPARDTFIKKELSGGGPMFDIGVHILDLTYWFIGCPKVHSVKGITYTKLAKRKDIRGL
                     WGEWDRETYNVEDFASGFIRFDDGRSLVLTCSFLANMEKLEDFSTTLFGTESGFLWPD
                     GKIATEKNGILQDIKPLFDNVMSDIQPPHHQEIKEFFECVKENKEVPVKLEQSLEVIK
                     MLEGIYISAESGKEVSF"
     gene            complement(27041..29128)
                     /locus_tag="PLW95_00830"
     CDS             complement(27041..29128)
                     /locus_tag="PLW95_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013756762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOV21214.1"
                     /translation="MLKQFFMPESIALVGVSTNPEKLGYKILKNIIDGGYRGKIFPVN
                     PKSGKILGLECYKSISEIKEKVELVVIVVPAKFVCDVAKECGEKGVKGLIVISAGFKE
                     AGEEGRKREEELEKIIKKYNMRLIGPNCLGIIDTTNDLNASFAFDMPQKGKIAFITQS
                     GALGTAVLDWAIKENIGLSKFVSFGNKADISEIDLIEELSDDPDTNVILLYLEGIEEG
                     RKFIEVARDVSKKKPIIIVKSGITEMGSKAVSSHTGSIAGSDIAFEASFKKSGVIRAY
                     SVEELFDYAIAFSYQPLIKGDRIAVVTNAGGPSVMAVDAIEKNGLKLAQISDTTKEKL
                     KEFLPPSSNINNPVDCLGDVMAENYGKALNVVISDKNVEAVICILTPQVVTEPKETAD
                     KIIDVSKGFNKTVVGCFMGGKRIEPGIIRLSEGKIPNYPFPERAVSSLKGMVNYRKYL
                     EKKEEKIITYKVNKEKVKEKLDNFKKAKIKVVGDIEGREILSLYGINTINSLIAKNLE
                     DGKNIFKKMGYPVVMKLVSPDIIHKTEVGGVKVGIKTESEFEKAFNDIIRSVKEYKKD
                     AKIEGIQIQQYIEGGIETIVGVNKDAQFGHLIMFGLGGIYVELFKDVSFSLVPLMENE
                     VSDMIDDIKASKLFNGFRNIPPVDKEKIKETLLRVSQLVSDFPEIKEMDINPLIVKEK
                     EVIAVDVRIGFDS"
     gene            complement(29491..31701)
                     /locus_tag="PLW95_00835"
     CDS             complement(29491..31701)
                     /locus_tag="PLW95_00835"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOV21215.1"
                     /translation="MKIGKNDKYKINIFFPSGNGIIQTETAILELKTFFKKCGLEEVN
                     SLSPEDLNIILSYPEKGIVQEGSFCLNISPSKREIKISGCDEEGIRCGIIYLLELFGV
                     RYFRPDSPALEEIILPIKLDETNQTFSPSFPYRCLHICAGKHHYDEKVAEWMFHLKMN
                     SKLTHHNEVDILKEELPKWGLKPDTTVHSYSFWIPDNRYFEQHPEYFALIGGKRVKQS
                     EGGQLCLSNQQMRTEFIQNILKYIKEHPDISIVGIPPNDGYGWCECENCKNLDTQEDR
                     KKGSVNGRVADFINDIANKVKEVYPDVLVGHYSYSNFSDFYQVKDVLPDNLIVSCTIW
                     RCYKHSIGDPNCPINKPIFERIKQLRRKIKHIYIYDYYTYNWDFLPAPIWQVVAQDIK
                     YYHQLGLDGFLSEVPGVESKAWETFHLPIYVTAMYLWNINTNLDNTLNDYCQKNFGPA
                     DSSMRRYLDCLEKGLKEMDGCFTKKTDDFKKMFTKEIQKECLGNIENALKESKNFPEY
                     YQRVNKEKELFDYWIHIFEEREKYHSQSEIKSFPLSQMNIETPLQKDPSTLVLVDKIT
                     LIPPEKNQTLVKVYSDNKEIVFIIECQEEEMENLTIHKGNDVGAVYGSDCIEIFIASS
                     QDAKTCYHFLINPDNFKCASECDLLTNRWNWSWQGNYQTETKKFPDRWVINFRIPKKE
                     VGIEGNKVYFLIVRNRKIKKWEITSFPGVKGYFNPKSYSSAILNSDLNRDCCVK"
     gene            complement(31719..34823)
                     /locus_tag="PLW95_00840"
     CDS             complement(31719..34823)
                     /locus_tag="PLW95_00840"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOV21216.1"
                     /translation="MAESEIGICGWRSKTWKEFLEKECEVKSDILKEEDGYIPVKEFK
                     NYKFIILSQDRPKPLTEEDNEVVKKYLKEGGHILITTITIGNWVPSDNRKFSSWADWI
                     GATTYQYGKVSAKIISPENPIVNHFAKQEYSWFGSEPGLTDITAGRVIIGTESIGKVF
                     INQYGKGTFIYFSPVVMSYYKQDWKDDPESSSLLLMVKKIVSSILKNEPPIEEIIKSS
                     KGQKTEIISVAGKKKEVIIISDSSSISIARTLCNYLNKITSGNIKVILETEKAEDIKE
                     NFKIYVGRTNYVNEIGLDFDKLHPYGYYIISNPDSLVIAGKYLGGTSYAIYDFLKRFC
                     GYRYFMPGELGEIISKKEIINLPLKIDIKEEPSFITYTNAGFYGGNGNFSRSWRTTFL
                     ASHNLYHIYSPEKYSESHPEYYPMIEGKRFIPPKNSGGTWQPCVSNPDLPEIAIEWSN
                     EYFEKNPQMLGIPVGVNDGGGDCQCPDCLELKRKFKNQYIPFYNQIAKLAQKQFPDKL
                     VSFIAYGGASPVPENIKLEPNIYVEVASGLRENMKLLEGWSNAGAKNIGIYDYLYGGG
                     YIVPRHYPHIMGKAWKEAYKSVNLKGGWFETFTQVWLYDGPRQYVLNELAWDIDADID
                     KLLDDYFLNFYGEANKPMREFFDRIEEIYGRKKDPLHPIADWQSLKQLDEYIWDDLEY
                     LNNKLNKAKELLKDEIVKKRIDLFEKIWRLSEFYLESYLTMKDMQNIEEIKKDEKIED
                     IINKAKMGFKAIESIENYQMTTEEEKNIFVNTNLESFKSQQTLIIKPYFENETYKIFT
                     KITEYFEKKGNSQEDIQSFWKKVAEENKDNEIKSIALSQIFIKQSPESKQNLVLNGSF
                     EPGEETKQEEVFSEEELEKFDWKKLDKRLKGWTTWNFQQSVTRFYWDPTQAHTGKYSI
                     SIRENQISGCFQAGIKVTPKAGYLLSFWVKQDPPDKGGSMTIRWMDKNGWADQGKGKA
                     PRISISYPKERESKWQKVEFFFIAPEDITSCVLLFGAPVQKQDECIWFDDISLIKVYD
                     PVFF"
     gene            complement(35566..36495)
                     /locus_tag="PLW95_00845"
     CDS             complement(35566..36495)
                     /locus_tag="PLW95_00845"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOV21217.1"
                     /translation="MDSCLIFSGNSNKNLAENICKYLNKEIGKINIYRFKDGEICVKI
                     EENVRGKDVFVIQSTCPPVNENLMELLIILDALRRASSKRITAVLPYFGYARQDRKDQ
                     PRVPITAKLIANLIVSAGANRILTMDLHAPQIQGFFDIPVDHLFAAPIVISYLEEKVF
                     DKLVVVAPDVGGVKMARAFAKRLDASLAIVDKRRESPDKVEVTHVIGEVKGKKVIIVD
                     DMISTGNTIIEAADILIKGGAIEVYGCASHAVFSEDTIEKLNNSNLKEIIITNTIPHL
                     SLPPKFKILSVSSLLGEAIKRIHLETSISSLFI"
     gene            complement(36480..37235)
                     /locus_tag="PLW95_00850"
     CDS             complement(36480..37235)
                     /locus_tag="PLW95_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOV21218.1"
                     /translation="MASNLAVVILAAGSGKRLGGEKQKVVKNILGKPMLSYLMETVKK
                     LFPDEVIIVVGFKKEEVIKQLEGENVKFAEQVVPLGTGDAVKKAMPLLENFDGNILIL
                     YGDVPFISYDTLKNFINDFENSKAMCSIITVSIDNPTGYGRIIRNKEGKITGIVEEVN
                     ATPQQKEIKEINSGIYIFKKQLLFDTLEKLKKDPVKGEYYLTDVIKILACQGVKIRSF
                     TIYTPEEVMGINTIEELQKAEKLLKRSEKWTVV"
     gene            complement(37252..37322)
                     /locus_tag="PLW95_00855"
     tRNA            complement(37252..37322)
                     /locus_tag="PLW95_00855"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37288..37290),aa:Gln,seq:ttg)
     gene            complement(37521..37907)
                     /locus_tag="PLW95_00860"
     CDS             complement(37521..37907)
                     /locus_tag="PLW95_00860"
                     /inference="COORDINATES: protein motif:HMM:NF015959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVG family protein"
                     /protein_id="HOV21219.1"
                     /translation="MEITETRISLVTNPSSRLRAYASVTFDNQFVIRDIRVIEGKKGL
                     FVAMPSKKIQRPCPKCGFKNPVGNKFCGSCGSALNPVEEQRLSPSRQHKDLAHPIKSE
                     FRDYIQKKVLEEYEKEKANPTKNNRS"
     gene            complement(37861..38784)
                     /gene="ispE"
                     /locus_tag="PLW95_00865"
     CDS             complement(37861..38784)
                     /gene="ispE"
                     /locus_tag="PLW95_00865"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOV21220.1"
                     /translation="MKVLAPAKINLFLEVGEKKEKFHPIVSLITPISLSDIVEIEKSK
                     KTEIIFHSKWEIPVDNTVKKTLDLLETKFKTDIPVKIDIWKRIPPGSGLGGGSSNAAV
                     VLENVIQMFNLDISEKEMLKIALNVGSDVPFFIYKNLSIVSGYGEIVKPVKNYIPTYY
                     FLLLVPPIRISTKDVYEQLDKKREFGNLTNGLKKIKILVGLLREREFEKAESIMYNRL
                     EKPCFEIWEEGKEVMKEIEKMSGRKFFLSGSGGSLFSIFREKREAETIKLGDVAVGWD
                     FFIIECFNNSKIKEEINGNNRNQNFARYKSE"
     gene            complement(38756..39331)
                     /locus_tag="PLW95_00870"
     CDS             complement(38756..39331)
                     /locus_tag="PLW95_00870"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOV21221.1"
                     /translation="MENKKEGIVYIKEKDLMKLKESLKKTKEIKKENEKLKKDIDELK
                     DRYLRVLAEFDNYRKRVEKEKQEIYKYGAENLLRQLLQFDNIFENILKQMETNPSNEA
                     IHKGVELLKKEFTKFLEENGVRKIVTKGKKFDPHIHEAIGVVETNEFEDGEIVKEEQP
                     GYVYHDKILKPSLVKVAKKPKTDESSCTGKN"
     gene            complement(39489..40028)
                     /gene="lepB"
                     /locus_tag="PLW95_00875"
     CDS             complement(39489..40028)
                     /gene="lepB"
                     /locus_tag="PLW95_00875"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOV21222.1"
                     /translation="MKKDIKKETTDWFKSLFWAIVIAVFVRIFFVGNFKIPTTSMVPT
                     LMVGDRLISNDIIYKIRKPQRGEVVIFKHIKETHTTDYVKRAIALPGERVLIKEGKIY
                     INGKEVTEPRISCRYYYSDGYYGVAKETEVPPGSYYVLGDNSINSSDSRYWGFVAEKD
                     LVGKALFIYWPPWRMGFIR"
     gene            complement(40060..41157)
                     /locus_tag="PLW95_00880"
     CDS             complement(40060..41157)
                     /locus_tag="PLW95_00880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOV21223.1"
                     /translation="MEDKKIKILYLLNSSEIGGTEKSLLLLIDNLNKKKFDIYIICLR
                     GEGTFTDEIKKRNIFAYIYDLKKEPLSIFIVYKKIKKINPDICQSFLFISNLIGRILG
                     KIAGIKIIISSQRSVDKWRKWYHWKIDRLTSKLSTIIISNSFSGKKYLVEKGKINEKK
                     IVVIPNGVKYPTEKRTFSKSSFGFSENSIVIGNVGNLRKVKGQEIFIKICKVISENFP
                     DVKFLIAGEGPLKVYLKHLTEVFGVKDKFVFAGFVKEIERVYSAIDIFVLTSQWEGCP
                     LSVLEAMSFGIPVVSFSVGDVPYIIQNGKDGFVVKDRNSEELVNKIKQLLEDKNLKEI
                     IGINAKEKIKKEFTIDKMVERYSELYINLLK"
     gene            complement(41147..41764)
                     /gene="tsaB"
                     /locus_tag="PLW95_00885"
     CDS             complement(41147..41764)
                     /gene="tsaB"
                     /locus_tag="PLW95_00885"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOV21224.1"
                     /translation="MKLLSIETTSSIGSIAVVENFNLLKEITFKSDDIASCLVENLEN
                     INCEIKTSDFDYFIVSIGPGSWTGIRTGISFVKGIACGKKEKIYAVSLPDSLFFILKD
                     FRCKLICVVNAYRENFYISKYNCKFNFKKSFPIKTFSKKIVKNILKKEDYIPIGPGVN
                     IFLNNYKKDVFKFPVYPLASYNAFLAYEKIKRGIKSPPLEPYYGR"
     gene            complement(41766..42848)
                     /locus_tag="PLW95_00890"
     CDS             complement(41766..42848)
                     /locus_tag="PLW95_00890"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOV21225.1"
                     /translation="MKKIIIYLAIFVFLAGCTGKKNKEVTLEEIPDVNYPPEEAPLST
                     DEISLTIEENVQEETIIQPEINLSQEEKKLEDERSLIAKKEQKLPNPVIPDEKEKKQD
                     GKFIELLNYFTSWSGEYLLYSAKWNFVNVGKGIIICKEEKNNNGEFYHIVGITVPEGS
                     MAKSGYGYNRVDSFIDKKTLKPYYFYSYVKNGEKERTTEIFFNFNKSEYTWNVKKLKK
                     GAVYSNKTQKVKFNGNLYDGMFIFYVLRSADFESYQKFQYPVAMEKIWNLTITRKDKM
                     TKDVPLLGNKEIYLLEPIAESDEGVFRKGKMNIWLTTDKERVPVYLEGRVTLGTAKLS
                     LISKAKINPKEKLDRALISRILNSVD"
     gene            complement(42950..45835)
                     /gene="mfd"
                     /locus_tag="PLW95_00895"
     CDS             complement(42950..45835)
                     /gene="mfd"
                     /locus_tag="PLW95_00895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOV21226.1"
                     /translation="MKLMEKVNSILGLIEKRDLFSIVGNNLSFFSFLISNIFKFSNSN
                     FLVILPDEKEAMIFYSDILNFIDEKEVIFFPEYEKVEDEISEVNIERVKSIIEIIENK
                     KSLIITYFPAILNKIPPFSNFKKIEVEKGENIRRDDFIEILVKFGYDKEEVVEIQGTF
                     STRGGIVDFYPPFSDFPLRVEFIGNRINSIRKFNPSNQSSFEKIEKCSILPLSEFFVG
                     ERQESFISQVNGINFIIRGEKFIEKNGEIQDIIEKSNIVGEKLTFLRNKNIFYFNVFP
                     AGDRFKISSQFIWNTYPGENISIFSQTESQNQRLKEILIERNIDISNIEFLKGNLSYS
                     FSIPEENITYISNDEIFGRYKVKYTPFKKRNFQNITEWEEIKKGDYIVHYNEGIGKFV
                     GMEKMEFDGEKKEFIVGEYDGGDKLYIPIEQIQFVNKYVGDKKPQLSKLGSKNWINIR
                     EKVKNSIRDLASDLYTLYVERKKERGFNFVSNDKMENEFEESFIYKETEDQLKAIEEV
                     KKDMESEKITDRLICGDSGYGKTEVAMRCAFKSVLSGKQVVVLVPTTVLCLQHYLTFS
                     ERFVDFPVIIEQLSRLVSEKKQKEIINKLKEGKIDIIIGTHRLLQQDVKFKDIGLLII
                     DEEQKFGVVHKEKIKAMFRKVDVLTLTATPIPRTLYMSLSGLKDISLIETPPEGRLSV
                     ITYAGIYNEGIVKQAIKKEMERGGQTFYLHNYVYDIEKVKEKIKKLVPESKVEVAHGK
                     LKPEKLSEIMRSFSKGEIDVLVATSIVENGLDIPGANTLIVDDITRYGLSDLYQLRGR
                     VGRYKWRAYAYFFIPKHIPLNENLRKRIDALQKLNSPGSGYKIALKDLEIRGAGNILG
                     KQQHGFIDMVGFNLYCRFWREVMGEIKGQEVKQPEEVEFPFILPDDFAPFPSFRFYIY
                     KKLSEIKTEEEKSKFIEEIEDKFGYLPEKIKTLISKKNSINYSEIEI"
     gene            complement(45850..46398)
                     /locus_tag="PLW95_00900"
     CDS             complement(45850..46398)
                     /locus_tag="PLW95_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HOV21227.1"
                     /translation="MVKIGDVEIEVIRGDITEQADIDVIVNAANAYLSPGGGIAGAIH
                     KKAGPKLYEECRKLAPIKTGEAVITFGYNLPNSYVIHTLGPVYLQEKGPAAKLAECYK
                     NCLKIADGKNLKSIAFPAISTGVFGYPTKEAAIVSIKSVIEEIPKLKSIKKIRFVLFD
                     ETTYKIFKETLDFGNIWSTGKF"
     gene            complement(46435..46908)
                     /locus_tag="PLW95_00905"
     CDS             complement(46435..46908)
                     /locus_tag="PLW95_00905"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOV21228.1"
                     /translation="MQIKIRKARSEDIEEIVKMTEKVWDGYTIAELLEKKYGKIAGKE
                     WYQYKKREVENFCKSHIDWVFVGEVNGKIIGYSTYTLDFERKIGEVGSNGVLPEFRGK
                     GIGSSLHKKVLEEMRKEGMETAIVSTLEINIPAQKMYIKHGFQKLVKSIFYTQKL"
     gene            47016..48140
                     /locus_tag="PLW95_00910"
     CDS             47016..48140
                     /locus_tag="PLW95_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOV21229.1"
                     /translation="MIYRKFGKTGINVSILGFGAMRLPMIEKNGEKHVNEEYSIEIIQ
                     KSFSYGVNYIDSAYGYCSGESEIVVGKALKGWREKIYVSTKVPTYNIEKRDDYRRFLE
                     EQLKKLDVDYIDFYHFHLLDNTKFEEKVIKLKLIDEAEKAKEEGLIKHISFSFHDKPE
                     IMKKIIDTNFFETVLCQYNILDRSNEEAIEYARKKGVGVAVMGPVGGGRLEKADELVK
                     FFKNKYHSSPEIALKFVFSNENVSIALSGMNTIEIVEENCNIASGGKFLAQQDKKLLE
                     KWIEERKRKKEIPCTNCKYCMPCPFGVAIPDIFNIMNYYKIYGLFDYAKKAYNNIGKW
                     EKDERKKANACVECGQCEEKCPQKLEIRKLLKEIHNTLFN"
     gene            48264..49091
                     /locus_tag="PLW95_00915"
     CDS             48264..49091
                     /locus_tag="PLW95_00915"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOV21230.1"
                     /translation="MKFSGISDEAGQSIEMQIKAHKELGWKYMELRNVDGENLTMVSD
                     EKFEDVYRKVNENGLLVSCFASGIANWSTKISGDFTKDVDELKRAIPRMKKFNTKFIR
                     VMSWPNDKDNPWEEKKWGEEVIKRMKQLSKIAEDGGVILAHENCSGWGGLSPENSIRL
                     LKEVSSPAFVLLFDTGNVIPHANMDTWDFYIKIKPYIKYVHVKDYKADKSGATFPGEG
                     SSKVKEILTDLKNSGYDGFVSIEPHIASIVHEGKVGDPELTYRSYIEYGRKLMQLIK"
     gene            complement(49100..49546)
                     /locus_tag="PLW95_00920"
     CDS             complement(49100..49546)
                     /locus_tag="PLW95_00920"
                     /inference="COORDINATES: protein motif:HMM:NF024879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HOV21231.1"
                     /translation="MKRGIAVLLIAGMLLSGCASVEWSSEKGKKGAGIGAISGGILGA
                     IIDSNKPWRGAIIGGATGAIAGALIGDSMDKNNVVETKEDIVSRAAKEAATLNTTVKY
                     SRTTENGITEEIIATPGKLEGNERTVTIQYLRDGKLISTETRKVKV"
     gene            49660..50106
                     /locus_tag="PLW95_00925"
     CDS             49660..50106
                     /locus_tag="PLW95_00925"
                     /inference="COORDINATES: protein motif:HMM:NF014037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HOV21232.1"
                     /translation="MKFIADGMLGGLARWLRFIGYDTLYFNTSRKIDFIRQAKKEGRI
                     VLTKDRKLCRDFPGLVYFVNGENTLEQFKDVIKNFKIRIKEKDIFSLCSICNKKLERI
                     EKEKIKGQIPEYIYLTKEKFSICPDCGRIYWDGDHCKRIKKVIKEI"
     gene            50103..51167
                     /gene="amrS"
                     /locus_tag="PLW95_00930"
     CDS             50103..51167
                     /gene="amrS"
                     /locus_tag="PLW95_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOV21233.1"
                     /translation="MKKIILIFSLLSIVLISSPKEALFYKKIEGNFVQCFLCPRKCVI
                     KTGEYGFCRARKNIDGTLYSMGYSNPCSVAIDPMEKKPFFHFLPGTDTFSIASAGCSL
                     RCKFCQNWQISQYSPEETKNFNLPPEKVVEYAKKNNCLSIAYTYSEPVNFYEYMFDTS
                     KIAKQNKIKNIVHTSGFINAQPLEQLCPYIDAVNVDLKGFNEEYYRNICQGDLQTILN
                     TLKIFKKHKIWIEITNLILPGYNDKPEEIEKMCIWIKENLGDYVPLHFSRFYPMYQMK
                     FLPSTPVSTLEEAYKIAKKVGLKYVYIGNVPGTKYENTYCPYCDKLLIKRVGFSVLEN
                     NIVNSKCKYCGKKIEGVWEK"
     gene            51164..52618
                     /gene="amrB"
                     /locus_tag="PLW95_00935"
     CDS             51164..52618
                     /gene="amrB"
                     /locus_tag="PLW95_00935"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOV21234.1"
                     /translation="MKKFILFFVLIPFLLYSENVRKPNFAGSFYPENTDSLSKMINNF
                     LKNVDNKKIKVEKDKIIGVIAPHAGYIYSGQVAAYSFKTLEDMTNKTFIIIGRSHHAY
                     FKEVIIEDRDFWQTPLGKVEIDKSLFEELYAQKQFHINHNLLDIDHAIEVEIPFLQKA
                     CKNFKIFPILLGDLDNENINKISSIIYNVIKKRKDIIFVVSSDLSHYYPENIAEEKDN
                     LLFKYLKTKDTNLIYNKLKERKEVEACGDAAILLLMKIAEKIGSYRIDVLSHSNSGKI
                     TGEKERVVGYGSIIILKSEKKEENMLTKEQKEKLLKIARQTLEFYLKNKPLPEIKETT
                     PVLNEKRGVFVTLKKRGNLRGCIGYIYPVVPLIEGVRKMAIESATGDPRFSPVTYEEL
                     QDVEIEISVLTVPKRVKSSDEIVLGRDGVIVKRGFNQGVFLPQVADETGWSKEEFLSQ
                     LCWQKAGLSPDAWKDPETELYTFQAEVFSESEFK"
     gene            52615..53238
                     /locus_tag="PLW95_00940"
     CDS             52615..53238
                     /locus_tag="PLW95_00940"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOV21235.1"
                     /translation="MKLHGNFFLSGIIFGFSFCLINCGLLLFPVVGEGSHNYKQGLLK
                     GILFNFGKIFSYSFYGGIASYSHFLLKTFIESKYSFIVGGIFLIIYGIWFFLKTGGKY
                     VVAGKFQKINLHIFLLGLIYGLVPCGPLIGFFLYVFYVSKGILFGVISGFLFGLGAIF
                     GPLTLCIFIPYIWKFLYRSSFKIFLRLISSFIFIFWGVNLILLSLSH"
BASE COUNT        18143 a         8587 c         7336 g        19332 t
ORIGIN      
        1 gagcctctcc ccgcagggga gagggaagaa aggaaggaag gagggaaata gggggaggga
       61 tataaagaca tataaaaacc ttaaaagaca aagaaaggca ttataaaagc tatagccaat
      121 tttggcagaa ccattttata cacaaggaga aagaaaatga aaacaaaaaa ttttatactt
      181 attccaagga aatattatga tatttactac ggaagagaaa atgttgcaat taagtcaata
      241 aaagaactac atggcaatga cattgggatt aaaattgtgt tatattcacc aaataacact
      301 gtctcccatt ttttattttc tactgggcag aatgatttta gtaaactcaa aaataacaca
      361 attactgtga aatttattga taatgaccaa attcaagatg ctatatataa aataaaacct
      421 atttttaaag atagtaaaga aactgaaatt tatacaataa atatcaaatg ctatccatca
      481 aaattttatg catcccagaa aatgacaggg catcctcaca tcatagcact ttataatgac
      541 cctattctta atattggaac tggttcagta gatgactggt tggtaactcc tactgaagaa
      601 gaaaaaaagt ttgccttgaa caaatggggt tccttagtag agaatgaaaa aacagattat
      661 gagaaagcca aaattttagc caaaacactt atgcatgacc tttggccaca ttctggattt
      721 ccttctgatg caatgaatgt tcctccattt gagcagtata aaaggatggt atcaggcaaa
      781 gataaaggat attgcagtaa ttttatggct atttttgttc atgcctgtaa ttcacttggt
      841 attcctgccc ggagttgtgg aataaatgaa atttatttta atcgtaataa ttcggaaaaa
      901 tgtaaaaaga tgaatataca aattcaagga ggctcttgtc atggcacaac tgagattttt
      961 gataatggtt taaaccattg gatatggatg gatttaaggt attatgcctt aggtgcctgg
     1021 ataggtaata taggacctct tactttagca gaatttcaga tatttataaa tcaaaaacaa
     1081 agacgagaaa aacttaaact tctcatatat aatatggaaa acaaaagtga aaaattgcta
     1141 cctctcaaca aatgcccaaa acaggaattt agttgtttta ctggttggga tgtgaaacta
     1201 aattatcaaa aaatgaaata aatggaaaat atacaaaaaa gcgaaaaaat tcaagttagt
     1261 tatgaagata caacacaacg gcaacatctt tgcataccag caatagaaac atctatgagt
     1321 agaaggtatc tatcaattat gcaaaattgg atctcatttg gtatggagta ttttcacgat
     1381 tggcaagttt gtccacactg tggacatttt tttggtggag tgttctggta tgggatggaa
     1441 acaagttccc cattatttgt cattgctgcg gtggtttctt ctccagaata taaccaaaaa
     1501 gtaacaggat atacaaaaaa gcaactaata aatgtagcag ttaaggcact gcgttatctg
     1561 tgtttcacac atgatacagg tcctcaagat tgtgtaagac ctgaaaaagg gttaggaaat
     1621 gccactcttt ttggaacaaa atggggagag aaaggaaagg gatttttccc tgaaagtcag
     1681 tgtggtgtta ctttatataa gatagtaatt tctgcattaa tactaaggaa atggattgat
     1741 agagaaacat ggatgatgat agcaaaaatt tgtgctgatt acttggagcg ttttgtagat
     1801 atgtctccta aatcaggagt ttattatgat actcaagcag aagaaaatac ttggacttcg
     1861 ttagggctga ctgctgcaat gcttttttta gaaaatcata aagatgcaaa aaaatgggaa
     1921 gaatctgcaa aacattggat gttttgtgct gctattgttc ctgatgacct acatgatgat
     1981 agaaaaatag acaagataga taagaagaaa agtattagag aactatgtag taagaaagtt
     2041 acttttcttc cagattttat ggcagaaaat cacggtatag tacatcctaa ttacacagca
     2101 tctgttattt taggagatat tatcaatcta taccgtttat ttggaagaaa agagccctca
     2161 cactggtatt ggcaccggca ggaagtatat gataatttga aatttctctc tgataagaat
     2221 ggtatagttc atcctgtgca ggggatggat tggccttatt taacaccttc tggtttacca
     2281 agtgttcatt gttttgctca cttatacctg aaagataaag aaggggcata ttttgaaaat
     2341 gctgcattaa atcttgcaga aaaaataata aaaggtaata aaggaagaat gtggcatcct
     2401 gatatagttc aatattgtca taatatacag gaccctatgc ttttatggga acaccagatt
     2461 tgtaattttg ttcctcttta tcttgctcat cgtatatgtg caaaagaaaa aattatgaaa
     2521 gtcccttctc aaaaaattat ttttgctaaa tatgttgggg ttagaaaata tcctcattca
     2581 ggatttattt ttcataaaca taaaaatgga cagacctcat ttagttggcg caatcagatt
     2641 atggcacttc ctgttacatc tgatggtttt ttgacaattg ccccctcatt taattctata
     2701 ttggggaaaa taaaagttaa gggatatact gaacatttta aagttgaata tatacatatc
     2761 aatgaaaagg aagatggttt tacagccatt atgagggtaa tacttaatga tggatgtatc
     2821 aaacaggata ttatgcttgc ttcattgcct gatggtaagt ttgtctgtaa ggaggttttt
     2881 tgggcattga agggctgcac tgtggaatat attcaacaag gatatattga aataatgaat
     2941 gagagatttc ctgaacttaa aataaactgc acaggttctc gcaagttata tactccatca
     3001 ggagtgtata actttccaag tatggtcaaa ccaaattcca aatatgacaa aaaagtagaa
     3061 cttaaaagcc ccccctggct taatcttgat gataggatgg gtattattat tgcatctgag
     3121 gggcaccaga cactttacca taatcgccac ttttttaaac catatcacgc tgttaccgat
     3181 gaactctttt tgaatatgca taacaagaaa aatttttata aaaaaaatag tataattgca
     3241 gatattttat tcatgtttat ccctggacaa aatcacaagg ataccccaaa acaattattt
     3301 atcacacctc tgtcttgctc tgaaggtatt tggggagctc ttacagataa taatctcatt
     3361 gtaggtaatt tagatgtttg ttcaggttca tattctattg aatttaaacg tcaaaaaact
     3421 atatctgttt ttcccggaat tacaaaaata gaaaatggaa aagtatctta tgaagttgat
     3481 ataattaaag ggcaatcact tttcatggag tcattatttt ctatagaaac caacggattt
     3541 tttatagtat ctgcgttacc aggaggactt gtttatataa caaacaatgg gcgagccaaa
     3601 gaaattcttt ccttggacgg ggaaaaaata tcagttgatg ctggtaaaac tatacttcaa
     3661 aaaaaaaggt agataagaat tagtaataac ttttgaagta aaattgaaaa ttcggagaga
     3721 aataaatggg aataaaaata ataaagattg aagaaagtgt ttttgcagaa aatgataaaa
     3781 ttgcacagga gaactataaa ttattagaaa aaaaaggaat tatttctttt aattttatgg
     3841 gctcccctgg tagtggaaaa acaaccataa ttgaagagat gataaaaaaa ttaaaagata
     3901 agtataaaat aggtgttatt gaaggtgata ttgccggttg tttagatagt caacgagttg
     3961 aaaaacttgg tattcctgtt gttcaaataa atacaggtgg tgcatgccat ttagatgcaa
     4021 atatggttaa aaaagggatt gaaaaattac cccttgaaaa tcttaatatt atttttattg
     4081 aaaatgttgg aaatcttgtt tgtcctgctg aatttaaatt aggtaataaa attaatatag
     4141 ttgttacaag tacaacagaa ggaagtgata agccagttaa gtaccctttg atgtttaata
     4201 tttctgaaat atgcctactg aataaaattg atatagaaga taattatttt gaaaaagata
     4261 catttttaaa aaacttaaaa aaggttaatc ctcatattgt tgtttttgaa atttctgcaa
     4321 gaagagggga taactttgaa aaatggatta atttttttga aaataaactc ttgaataatt
     4381 aagtattcca ttactttgtt aacggataac ctattacctt caaaaagttt tttctcttaa
     4441 taaggtttac ccgtctatct ttggagaggg gaaggatggg atggatgtag aaagagaaag
     4501 acaaggtaat tttggcagaa ccccgaaggg gaaaggagaa aaaatgaaga aattattttg
     4561 tgcagttgtt ttgagtgttt tatttgtttc ttatgggtct tgtgatttga aagagaaagg
     4621 agtttctttg actatttata accagaattt tgctcttgtt tcagatgtaa gagaagggaa
     4681 aattgaaaaa ggagagtcaa ttcttaaatt tacagatatt gcatctttaa ttgacccaac
     4741 aagtgtttct ttttcttcat taaattatcc ggggttattg agtgtaattg aacagaattt
     4801 tgaatatgac ctgcttaatc ctgataaact tttaaacaag tatattgaaa aaaatattca
     4861 ggtaattaca aaagatgggc aattgtatag tggacaactt ttaagtttta atagtggtca
     4921 attgataatc aagtcaaaag aagaaaatgt atttatgctc aacagagaaa atataaggga
     4981 tataactttt gttgaaattc cagaaggttt aattttaaaa ccaactcttg ttcttgaaat
     5041 aagaaataat aaagaggtat ctcatatact tcaacttaaa tatattactg atggaataaa
     5101 ctggaaagca gattatgttg cagaactttc accagatgat aaaaaaatgg atttaactgg
     5161 ctgggtaaca attgataata ggtcaggagt aacatataaa gatgcaaatt tgaaacttgt
     5221 tgcaggagat gttagaaaaa tttctcaata cagagaatat gcaatgcctg ctggaaaagt
     5281 tcttgctgca caggcagctc ctcaatttgt tgaaagtcca ttttttgaat accacctcta
     5341 tactctaaca agacaaacaa ctattaaaaa taatcaaaca aaacaaataa cttttctttc
     5401 ctcggccaat gttcctgtca ggaaaaagtt tatatatgat ggggcggcca atagatatta
     5461 tcattatgac agttggcgtt ctgtccctta caatgataaa gttgagattc atattgtttt
     5521 tgaaaataag aaggaaaata atttgggttt gcccatacca aagggaagag ttagatttta
     5581 taaaaaagat acagaaaata ctactcaatt tataggagaa gataatattg accatacacc
     5641 agaaaatgaa aaagttgaac ttataattgg aagtgcgttt gatataaaag gtgaaaggat
     5701 aattttagag cataataaaa tttcaagtaa tatctatagt gatacatacc agataaaatt
     5761 aaggaatcac aaaaaagaga aagttgaaat agaagtgatt gagcatttat atggtgattg
     5821 ggaaattact aaaggttcac aaaattatga aaaagttgat gctaatacca taaaatttct
     5881 tgttgaggta cttccagaaa aagaatttgt aataaattat acttctgttt ccaaatttta
     5941 actttcctac cccctatttt ttctaaggag ggagtaagta attttcccct ttttcaaagg
     6001 gggaagtttt tctccttcct ttataaaagc atgtccgcca taatacatgt cggacaggct
     6061 ttgttaaagg gggaagtatc tctccttccc ttataaaggg aaggtaggga tggattttat
     6121 taaaatctcc ccttgcccct cttttctaaa gaggggaata taaaatcccc ctcatcccca
     6181 ccttctcccc agtggggaga aggatgaaat ccccctaccc cctttttcaa agggggaaat
     6241 aaagcaccaa atctgaggca cagagtaagc aactttagtc ggtttactaa agggggaact
     6301 tatggggaag ggataagtca aagggagtga gagggatttg cctttctttt ttattctcca
     6361 cttctttatt tatcaggtac tggtttggaa ataaaatttg aatattcaaa ataagaaatc
     6421 tgccctggag caactttggt attcagagaa acataggtaa agagtgaacc aaaaaagata
     6481 ggaataaacc gaacaaatgg attttcgttc attggaatta taattaaagg gatttttttt
     6541 gagtaagtat atgtaatttt aataaggtca aaaagatttt tttctgtttt tattaaagtt
     6601 gcaattttta ctatatcacc aactttattt ccttttctta caatttcttt taactttttt
     6661 tcattcggtg tttttttaaa attgtggtat gaaacaataa cttttttatt ttgactttcc
     6721 acaatttcag tcacttcttt taaaattcta cttttaagtt ctacatcaat aaaatcaaca
     6781 taagtaatta tttttttaaa aatttcaagt cgttctttgt cttttatata atatgggttt
     6841 tgtccacttt ctttatacca tctaatagtt gcaataattt ttgtatttaa tttctcttta
     6901 atttttaata tccattcagg taattcatct attgtttttc tttttaaaaa ttcatcaatt
     6961 ctacattcaa ccaatgcaat atcttttaac ttttctatat cactgttttc ttcatttcct
     7021 gttaatacca cacatatctt cttctgtaag tcactgccca ataaatttcc cattttttct
     7081 tccttactta tctgagttgg tacgttatcg ttttctaaat aatacaaagg tagggatgga
     7141 tttaataaaa ttccctcaaa atcccccctc ctccgagctt tctcactccg ccataaatct
     7201 tgtcggacag gataagggga gaagaaaata catcccctct ctcttcccct ctccccactg
     7261 gggagagggt tggggtgagg gggaaatttg aaatccctct ctttccccct ttgttaaagg
     7321 gggaagtatc aaaatcccct ctaattccct ttaaaatccc tcttaatctc tccgccaaaa
     7381 agcgcgtcgg acaggccttt tataaaggga gaaattaaaa gggggaaatg acgcaccaac
     7441 tctaagaaac aaggtaagca actttagtcg gtttgtcaaa ggggagaacc cggttctcat
     7501 agagaaccat gttcttttat cttaacattt gtcaaaatgg ggaacaaagt attcttttat
     7561 taactgacac ttccccttat tagatttcaa aaccaatatc aagtttgctt tttgcgagaa
     7621 tataactatt ttgtaaaaca gtgttcacat ctgagacctt tataccagca gaaattatta
     7681 tcttttttgc aaattcatca tctattaaat tttctggtga atgagtatct gtatctaaaa
     7741 taattgggaa accatattca taccataatc taacaatatg accattagaa agacaatgtc
     7801 cttttcttgc acttatttca atataaattt ttttttcagc agcaagaatt gcatcttctt
     7861 cacttaaaat ccccgggtga cctaaaatgt caatatcact ttctatccct tttctatttg
     7921 ttcctttgga aactggttct acaattgttt ctccatgaac tactataagt tttgcaccaa
     7981 tgttttttgc aatttggaca agaacaggaa tatctattgg gttgacatga gtaatttcta
     8041 cacctggtat tgtctttatt ttgtaatttc tatttattct atcacaaact cttaccaatg
     8101 aggaaacaac catttcaaca ttcgaaaaat caatatggtc tgtaaaagca ataactttat
     8161 atcctttttc ttctgctctt cttgcaagtt cagatggaag tagacagcca tctgatagaa
     8221 ttgtatgagt atgaaggtca atcattttct ccctcctttt ctgttttttg ttttccacgg
     8281 gaataatgaa ctccatgaac aacaacatca actttaatta ctttcaagcc agttattctt
     8341 tcaacttctt cttttatttt tttctgtatt tgataggtaa tttcaggtat atcaactcca
     8401 tattcaataa taataccgag ttcaaaactt acttcatttt ctcttactat aattttaatt
     8461 ccagcatcat gtgtttttct aattaattgg gcaattcctc ctactaaact tgttataatt
     8521 ttataaacac ctggcacttc ttttgctgca attcccgcaa ttgttcctaa tacctcatta
     8581 tcaattctta cacttcctaa ttcaccattt tccatatttt cttctccatt ttaaacaatt
     8641 ttactataat gacttggaat attattcctt ttcagtatgt tctaactttt gtttattact
     8701 ttttttgcaa tgtaattttc aagatagcca acataatatc tgccgcttaa aaaaactggg
     8761 ttatccatta tttctttata aaatggaatt gttgttttta ctccctcaat cactgtttct
     8821 tcaagcgctc ttttcatcct tctaattgct tcatttctat ctcttccata agttataagt
     8881 ttcatcaata gggagtcata ataaggtgga acaatatatc cttcaaaaat atgtgtatca
     8941 acacgaacac caggacctga tggtagaaac agtttttcaa ttttccctgg agaaggtaaa
     9001 aaattgtttt cagggtcttc agcatttatt ctacattcta ttgaatgtgc ctgtattcca
     9061 atatcccttt gcttgaaaga taatttttct ccacttgcaa ttaaaatttg acttttaaca
     9121 aggtctatgc ccgatactaa ctctgtaatt gggtgctcta cctgaattct tgtattcatt
     9181 tctataaagt atggtttttt atctttatca acaagaaatt caatggtccc agcatttaga
     9241 tattttatat tttttgcaag tttatatgca tatttttgta attttcttct taattttata
     9301 ccaataacag gagaaggact ttcttcaatt aatttctgat gtctcctttg aattgaacaa
     9361 tctctttccg ggaaaaggta tatgtttcct tttttatcag caagaagttg tatttcaata
     9421 tgtcttggtc tttcaagaaa tttctcaatg taaaggcctc cttccccaaa agaaattttt
     9481 gattcttcat agcaggtgtt ccataaattt tcaaattcat ctgcactact tacaattctc
     9541 atacctctgc ctccaccacc agcagatgct ttaattatta caggaaaacc aatttttttt
     9601 gcatgatgga gtgctttatc ataattaaat tcttcatatc caggaattat gggaattctg
     9661 cattttttag cgatttttct tgccaatggt ttatctccca tggtttttat gttttcagcg
     9721 gtgggtccaa taaaagttat tcctgttgca ttacatatat caacgaactg aatattttct
     9781 gacaggaatc catatccagg atgaactgca tctgcttttg ttatttcaat ggcactaatg
     9841 atagaaggaa tattcaaata acttattgaa ggtgaacttc ctccaataca aattttttca
     9901 tcagcaaatt ttacaggata tgaattctta tcaacttctg aatatcctat cactgtttta
     9961 agttcaagtt ctttacaact tctcaaaacc ctgagagcaa tttctcctct atttgctaca
    10021 aaaacctttt ttatcatttt atttcaatta cgaaaaggac ctgaccaaac tcaactggtt
    10081 ttccattttc aaccaatatt tctttaatag ttccgtcaac atctgctttt atttcattca
    10141 taactttcat tgcttcaaga atacaaagaa catctccatt ttttacttct tttccgactt
    10201 caacaaaagg tggactttca ggagaaggag agcgataaaa agtaccaaca aggggtgatt
    10261 taacataaac aatattttct attttttgag tttgttcttc tgttttcttt aaaatatcag
    10321 atggatttat ttcttctttt gtttcccatt cacttttttt accaagaata agatgaaaat
    10381 cacctttttt gatttcaaga tagtcaaggt catatttttt cataaattca gcataaactt
    10441 ttatttcatc tgttttcatt ttattattta aactcttgtt atgtattcgc ctgttcttgt
    10501 gtcaactttt atcgtatctc caatttcaat aaataaaggc gtctgaacaa ttaaaccagt
    10561 ttctacttta actttttttc ttccaccttg gacagtatct cctttatatc cgggttctgc
    10621 ttcaataact tttaaatcaa cagttgttgg taagataatg tcaataattt ctccatctaa
    10681 aaaagcaccg tatgtttgta cattttctat taaaaacttc tctttttctc caacaatttc
    10741 ttttgccaca ggaaactgct cataagtatt actatccata aaataaaaaa catttccttc
    10801 tctataaagg taggttaaag gaatttcgtc aacgaaaaca tctccaactt caaaatcaga
    10861 cctgaaagta agttcaacag ttttgtttgt tttcatatct ctaaccttga taaatacctt
    10921 acctctacct cttgattgag taagatgact ataagaaagt actgaataaa tattatcttc
    10981 atatttaaat aaacttcctt ctcttaaatc aattactttc atatattctc caatttcttt
    11041 tgtaagggaa agacaaaatt tttaatctcc ctctttccct gttgtgctat ctgaaaactc
    11101 caaaatctct tttcaaaatt tccataaccc gttgtgctat ctgaaatccc actttataaa
    11161 gggggagaat actctccctc ctttaaaaaa aggagggcag gggtggattt taaaatctcg
    11221 ccctttccaa tgcttacccg tctatctttg tagagggaag gccggctcgc ccgtagggtc
    11281 cacagaattt ctctgggcta tgcccgaggg gatggatttt tattaacaat cccccttcat
    11341 ctccgttgtg ctatctggga gacgataacg caccaactct gcttgctaaa ctttgctacc
    11401 tcagtcgatt tgcttaaagg ggagaattaa aagggaaaat caaacaccaa ctttgccctg
    11461 ctattccccg ccttttttag tcagtttaaa aaagacagaa gggggattta atcttttgct
    11521 cgttattttt cagcaatata gttggctggt tatttattat tataacttaa atagacaata
    11581 aaaacaataa aaggggaaaa actgatttta ttattttttt ataaagatat gattttccac
    11641 ggagtgcttt caatataatt tgtgtatttt ttcatatcaa ttcctgtttc tttaaaactt
    11701 tcagtccatt tttcaaaatc aaaaaattca ttccagtttt ccatcttacc accttttttc
    11761 cagacattat aaattacctt tgatattttt tcatctcctc ttgcgagaaa acattcaaca
    11821 caactcatat tatatggatg aaaatcaacc tttacatatc tatttttttt atggctttca
    11881 gttataaaat ttttcttttg ttcatatcca tcaagagata taaatttttc tttttcaaat
    11941 tcagtatgtg gttttggaat aaatgtattg aaagagacat ttactttaat tattttagaa
    12001 atatcattta ttaaattaac tatttcagaa atatcttttt ctgtttcatc aggtagccca
    12061 atcataaagt aaagttttat atgtctccaa ccactttctc ttgcttcttg gcataattca
    12121 atcaattttg tgtttcccat ttctttacca attttatttc tcaaattttc tgatgtttca
    12181 ggagcaaatg ttaaagaact tttttttatt tcttttattt tgcctgctaa ttggaaagta
    12241 aatgtatcca atcgtaatga gggaaaggaa attgagacat ttcttttttt aaattcatca
    12301 acaagtaaac taacaatttt ttcaatttca ggatggtctc ctgctgaaaa agaaagaagt
    12361 gaaatttctt catatcctgt cttttcaaat gtctcttttg caatttcaag cactttttct
    12421 ggacttctct ttctcactgg tttccagcat tttcctgcct gacagaactt acaatttcta
    12481 taacatcctc tcattatttc aagagaaatt ctatcatgga taatatcagt cagtgggaca
    12541 aggtattttt tagggaaata agaattttca aagtcaaaaa tagttgcttt tttaatatta
    12601 ttttttggag acatagggac aaaaacacct tcaatttctg aaaggcattt tagaattttt
    12661 tctctttttt ctcctcttaa ttccctatat gtctcaacta ttttttccat tgcttcttct
    12721 ccttctccta ttacgaaaac atctatgaag ggaataattg gaaatggatt gaaaacacta
    12781 ttgccaccag caataataat tggctcatta ttacctcttt cttttgagaa aattgggatt
    12841 ttgcttatat ctaaaaggtt tagaacattt gtataattta gttcagaaga taaactaaac
    12901 cccacaatat caaaatccat aacaggcgtt tttgtttcaa gtgtaaaaag gggaatatct
    12961 tctttcttta aaatttcttc catatcttct aatggagaaa aaaatctttc acaaatacaa
    13021 tcatcccttt cattcaacat accatataaa attctgaatc ccaatgatga cataccaatt
    13081 gaataaatat ctggatagga aagggcaatt tttacctttt ctttttccca ttttttaata
    13141 taaacattta tttctttgcc taaataagaa gcaggatttt ttacttttga taataattga
    13201 gtaattttat tttccattaa aatacagacc aactatcctg aatatttttt aaaaaaatta
    13261 tacatatcaa acaggtaata agagaagaac caccataact gataaaaggc agcggtatac
    13321 cgacaattgg gaaaagtccc atagtcattc ctatatttat tgtaaattgt gcaacaaaca
    13381 gagttaatat tcctgttgca agaagtttcc caaattcatc tgttgtaaaa gaaataatct
    13441 caataatttt tttaaaaaag agataataaa gaaaaagaaa tattagaact ccaataaaac
    13501 caaattcttc tgctaaaaca caaaaaataa aatcagtgtg atattctggt aagaaagcaa
    13561 gttttgtctg tgttcctttt aaaaatcctt tacctataag tccccctgaa ccaattgtta
    13621 tttttgattg taagagatta tatccagcac caattgggtc tcttccaggg tttataaaag
    13681 taaggattct ttctttctgg tagcccttca tactaaacca taaaatagga cttaaaagta
    13741 aaacaataaa aaataaagat aaaaattgtt ttctactcat atctgcctga tacaagatac
    13801 caagagaaat gaatataaat ataaaagcag ttcctaaatt tggttgtaaa agaacaagaa
    13861 taaaaggtat aaagacaatc aaaagagatg taaaaaaaac agaaaatttt ctaatatttc
    13921 tattttctaa atatgcagaa agtgcaacta tcaaaacaaa cttggcaaat tcagatggtt
    13981 gaaaatatag ccaacccaaa tttatccatc ttgaaggtcc tttttccccg gtagcaagaa
    14041 ctattaaaag aagaaataaa attataaaat ataaaatggg ggcaaacctt tttaattctc
    14101 tataattaac tccatttagg aaaataataa taattgttcc taaaataacc catatacatt
    14161 gtttaacaaa aaagctcgac ttgaagataa ttccttttga agcactataa agaagaagta
    14221 atccaataat aacaagaata taagtcaaaa ttacaataaa tgtactatca tccctttctt
    14281 taaaatatct aaataatccc attttctatt tcctctgatt tctctattgg atttttattt
    14341 tcttcctggg tttcttcatt tgtttcgcca ttttcttcaa ttcttccttt ttcaggtatg
    14401 aagacctctt ttaaaatttt tcctgctatt aatgctgctt ctgcggaaga agcatggtca
    14461 agaaaaacaa ctattgcaat ttgaggatta ctgcttggag cataacaaac aaatccacca
    14521 taagttggta aatcactttc tccccctgct ctttgagcag tacctgtttt cccacttact
    14581 tcaaatcctt caacatttgc taaatgtcct gtcccaaaaa gaacaacatt tcttaatccc
    14641 ttttttaaaa tttcaagtgt atctgaactt ataaagactg ttttattgat aaaaggtgaa
    14701 cattctttta aaacatttcc atcaggtgaa acaatttttt ttacaataaa cggctcccag
    14761 atattacctc catttgcaac tgttgaaatt aaagatagaa gttgaattgg agttgttaga
    14821 attggaccct gtcctattgc taaatttagt gggtctgttt ccttttttaa aggaagatgc
    14881 ccgctttttt ctcctggaag gtcaattcct gttttttctc ctaaaccaaa tttacttgca
    14941 tattcaagca ttttttcaac tccaaccttc attccacatg tgccaaaaaa tatattacag
    15001 gaaaaaggta aagcgaggtt tatatcaatt aacccatgcc cctcatcttt ccagcaatga
    15061 aaaactctat ctcttatttc cattgctcct ctacattcaa ttctgtcata ttcttgaatt
    15121 tcgcctgtct ccaatgcaga tatttctgtt ataattttaa aaattgagcc aggtgggtat
    15181 tgacctttta taactctatt cacaagagga tgacttgcta aattaaaata tttttctact
    15241 tctcctggtt taaaaccggg tctactaaca agagcaagaa tttctccatt tcttgggtcc
    15301 attgcgacta cacaaccact tctatttcct aattgtttat atgcacttct ttgaataaca
    15361 ctatcaatag ttaaaattaa actgttccct gttttccctg cttgtttttt aagaactttt
    15421 ctgtaatgtc caagagcatc aacttctacc tgtattccgc tgggacttcc tcttaaaaag
    15481 gtatcatagt atctttctac cccgtctttt ccaatagtat ctcctatttt atatcctttg
    15541 tcttttaatg catctaattc ttcttttgaa atttctccta aatatccaac aacatgacat
    15601 gtctggtctt caagtggata ttgtctacca atactttcct gagggaaaat acccggtaaa
    15661 ttatcaatat tttcttcaat aagagaaact tcctcaatag ataaattcct ttttattact
    15721 attctatcat aaggatttgt atattttttt ttgaattttt caataagaaa gtacttatta
    15781 aaagaaatta tatttgataa aatttctgct tctttttcaa tattttttat gtcaaaaggg
    15841 ataaatacaa gattaagagt aggtatatct tttgcaagaa tttttccatt tctatcataa
    15901 ataactcctc ttggagttcc gatatttata gttcttatac aatttttagc agaaagttgt
    15961 gaataatatc catatcttac aacctgaaga aaaaagcacc agacacccaa aattattaca
    16021 agaaatttaa ctaaattgta aaaaagtctt tctttttcca tttcaatatc tttcccaaat
    16081 aaacaaaata ttcaacaatt acatttagta tagcaaaaat taatgaatat tttaagatta
    16141 cctgtttatt ccacataaat ccttccccaa ttttcattgg taaaattata aaatacatta
    16201 gactaaaaat aaaaaccaaa atatttctta taagaaaaga ccttacctta taaaaagaat
    16261 ttatatatac aataataaga aaaattatac tactaaaacc aggaattgat ttgttaattg
    16321 tgtcatatat tatgcctgaa acaaaagata aaaacaaaga aatttcaata ttcccttccc
    16381 acacaagatt taaaaggaat ataagtaaaa aacaagggac aacattaggg gaaaaataaa
    16441 ttgaaaatat aatctgaatt cctacaacaa ctaaaaaaac ttcaaaaatt ctaattatgc
    16501 ctttcttttc caattagtac ctctttaata taagaataca tacagtatgg tttaacataa
    16561 attttaagaa ataatgaatt tgtctgtttt tctattttta caactttgcc aatttttata
    16621 ccagcaggaa aacttttact ataaccagaa gttaagacct catccccaat tttaatatta
    16681 ctatcagaag aaatatattt taaaagaaca aatggagttg ttcctccttc aataattccg
    16741 acttcaccag ttgaaaaaag aacagaactt atttttgttt ttgaattaaa aatagtagaa
    16801 acataagata cattttcttc cactctttca actttaccaa caagattaag gtcatctgtt
    16861 ataacaacca tcccagtttc tacattttcc ttttgccctt tatcaacaac aagttcacca
    16921 ggaaataaac ttgggatgaa atttataatt cttccaacaa taaattcatt taattttcct
    16981 tcttcattta atttaaggat ttttttaagt cgttcatttt catttattat ttctgaataa
    17041 tttataatgt ttttcttgac caaatttttg gaaaaatttc ttgaaaaata taatttatta
    17101 ataagaaatg aaaaataaat taaaataaaa aataatattt cttttctaaa tcgccatatc
    17161 ataattttct attagatgtt ttgtgtaatt tattcttgag ggaaatgttt aaaatatttt
    17221 tcttcttcaa gcattttacc tgttcctcta acaacagcag taagagggtc atcagcaaca
    17281 attacaggaa gttttgtctc ctgagaaata agtttatcaa ttccttttaa taatgccccc
    17341 ccacctgcaa ttacaactcc tccttcttta attaaatcac tagaaagttc tgggggtgtt
    17401 tcttcaagaa catccctaac agcatttact attaaactta atgtgtcttt taatgcctct
    17461 cttatttcct ctgagtttat ttttatctgt ttgggcaatc cctctaccag acttcttcca
    17521 tgaacatcca tttcaagttc ttcattaagt ttataagcag aaccaatttg tattttaatt
    17581 tcttctgctg ttctttcccc aattaataaa ttatattttt tctttaaata ttcaattatt
    17641 gcttcatcca tttcattgcc tgcaattctt aaactttttg taattacaat acccccaagt
    17701 gatataactg ccatttcagt tgtcccccca cctatatcaa ttattaaact tccacatggt
    17761 tcctcaacag gcagtcccac tcctattgct gctgccattg gttcttcaac aattcttacc
    17821 atccgcgctc ctgcttttaa agcggtctct ttaactgctc ttctttcaac gcttgtaatt
    17881 ccagaaggaa ctgaaattac aattcttggt ggaaggacaa atttatgttt tggtgttttt
    17941 acttttgaaa tgaaatatct caacattgct tcgcatccat caaaatcagc aattactcca
    18001 tctttcattg gtttcattgc gactatatta gcaggtgttt ttccaagcat tttttttgct
    18061 tctccaccaa cagcaacgaa ttcttttgtg gtggtattca ttgcaactat tgatggttcc
    18121 atcaagacaa ctccttttcc tctgacataa acacatgtat ttgctgttcc taaatcaata
    18181 cctatatcat ttgaaaaaaa agtcagaaat tttaccatat tttctctcct tacatttctt
    18241 ctataatttt aaaaacttca tcgccatctc taacaatttc atctgtcttt attccaattt
    18301 catcaccaac ttttgcaact tcaacttcct ggtgttctat ttgtattgaa tttacttttt
    18361 gagaaaaatc tgttgtgtgt ccttttatat gtatctcatc tccccttttt aactggtcgt
    18421 tggtaatttt aatagcagca actccaattt tattaaaata atgtgttatc tcccctattt
    18481 tcatctcctt cattattcac ctccattttg ttcaatatat caataaaaac attaacaagt
    18541 tctctatcaa attgagtacc tgcatttgcc aatagttcac ttattgcttc ctctttactt
    18601 agtgcttttc tgtatggtct atctgcccgc atagcactat aagcatcagc aattgccaaa
    18661 attttagcat ataaagggat tttttctcca cttaatcctt ctggatatcc ccttccatca
    18721 actctttcat gatgatataa acatgcttca actttttctt tatccacatt taatggtcgc
    18781 aaaatattca cagttattaa aggatgcttc tttataaaat atttttcttc tggtgttaat
    18841 ggttcttctt tagaaagaat accatttttt actcctaatt ttcccaggtc atgtaaaaaa
    18901 gaagatattt ttagtgtttc tatttcttca tcacttaatt ctaatctctt tccaagaagt
    18961 gtagcataat atgcaaccat ttctgagtgt tgttttgtgt atccatcttt tacttcaagt
    19021 gattttatta gacactctat tgtatttaaa aaatatgagc ccctaattgt ttccaataaa
    19081 atagagtttt caattcctat tccaatttgc attgaaaata attcaagaaa ttttacctcg
    19141 tttttttcaa agatacctat gtttttagaa atttctatat caagcaatcc aacttctttt
    19201 tcttttcctt ttattctaac aaaagcacga attgttttag tttgttcatc taatttaact
    19261 atttctgaac caaagatttt atttatttct tcttttgaat aactcatttt ttgtttattt
    19321 gtcttatctg caatatattt atgttttctt aaaataaaat tgctactgcc tttatcaatt
    19381 aaatagattg aaattctttc gggtgaaaat gtttttaata aaatgtcaga tatattctca
    19441 agaaatgtat caatgtctgt aagaaatcca atatttttgt tcaactcaaa aagtgtctca
    19501 aaaattttta atcttgatac ttcttttaat aaacttccaa tttctatatt ttttttaatt
    19561 gttttaaaaa attgattaat atcaaatgga taaatcaaaa ggtcatttgc acctgaattt
    19621 attgctttta ctgcattttt aatttcttca tctattacaa caattggtat taaattattg
    19681 aattccctga cgtttttaat aaactggaat ccatcaattt cgcttatttg taagtcaagt
    19741 aaaataagag taaattcatt atttttaatt ttatcaattg ctacaaaacc attatttgtt
    19801 tcctcaaaaa taaaatttct tttttcaagt aaatccctgc ataatttttt taaattttcc
    19861 ttatcagata taattaaaat tttttcattc attttctacc tctctttgtt cattttattt
    19921 taacaagata atggaacaaa gtcaatataa taccatttac cctataattt ttgaattgtt
    19981 tattttttat actgcttctt cttatggtaa aattaaaaaa tgcttgaaac aaaaaattta
    20041 aaaaagagtt ttgggagaaa agtagccgtt agagatattt ccataaatgt tggtaaagga
    20101 gaaattgttg gacttttagg tccaaatggg gcaggaaaaa cgacaacttt tgaaatggta
    20161 cttggatttt taaaaccaga taaaggtaat atttttgttg atgggataga tataacaaac
    20221 ttttctgtct ggaaaagaag tcaattgggt atttcttatc ttccacagga aatatcaatt
    20281 ttttcaaaat taacagttga agaaaatttc tatataattc ttgaatcata ttacaaagat
    20341 aaaaaaaaga tgaagcaggt tgttgaagaa tatctaaaaa agatagggct ttatgatatg
    20401 agggaacagc cagcaggaac tctttcaggc ggagaaaaaa ggcgacttga aataggaaga
    20461 agtttaagtt taaatcccaa gttttttctt ttagatgagc ctttttcagg aattgaccca
    20521 aaaactgtat ctgaacttca ggatatagtt gtcaaattaa aaaattcaaa tattggtatt
    20581 ttacttacag accataatgt aagagaagca ctaacaataa cagatagagc atatataatt
    20641 tatcagggca aaatacttat tgaaggaaaa ccagaagaaa tattaaaaca tccagaagca
    20701 agaggtgttt attttggaga gaaatttcag atatgagaaa gaatttaacg atattaccgg
    20761 tacatgttgg aattataatg gatggaaatg gaagatgggc taataaaaaa aatttgccaa
    20821 gaatttttgg tcataaagaa gggattaaag cagtaagaag ggtggtaaag gcagcaaaaa
    20881 aatttgaaat taaatatctt acattatatt ccttttcaac agagaactgg aaaagaccgc
    20941 aagaagaagt ttcttttctc cttaaactta tagaaacaag actgataaaa gaaggaaata
    21001 atttaaacaa aaataatgta aaagttgatt ttattggtaa gaaagaaaaa cttcctaaaa
    21061 aacttattga aataatggac agggtaagaa atttaactaa aaaaaataca ggacttactt
    21121 taatttttgc tataaattat ggaggcagac aggagatatt ggatgcaata gaacaaataa
    21181 ttaaacatgg gaatataaaa aaggttgaag aagaaaattt ccggaatttt ctttatcttc
    21241 cttctcttcc tgaccctgac cttatagtaa gaacagcagg agaaaagcga ataagcaatt
    21301 ttttattatg gcaatcagca tattcagagc tttactttac agatgttctc tggcctgatt
    21361 ttgatgaaga ggacttttat aaatgtcttg tttcttacca aagaagaaaa agaaaatttg
    21421 gggggcttgt taagtgaaaa gagttgttat ttttggttca acagggacaa taggaataaa
    21481 tacacttgaa gttataaata gacaaaaaaa taaatttaaa gttgtcgctc ttgcatgtaa
    21541 agaaaacaaa aaaattcttt ctcaacaaat tgaacaattt ttacctgaat ttgtttatat
    21601 tgaagaaaga gatacatctt ttgaaaggaa atttaaaaat ataaaatttt tttataaagg
    21661 agaaatgtct gaaatttcaa aagttgattc agatattttc atttttgcta tacctggtat
    21721 tgacagttta gatacctttg ttgagtgtct gaaaaggaga aaaaaaattg gacttgcaac
    21781 aaaagaaatt cttgttgttg caggtagtat tataaaaaat ttattaaaaa aatacaaaag
    21841 tgaaattctc cctattgata gtgagcataa tgcaattttt cagattatag aaaaagaaaa
    21901 taaaaaagat attaaaaaga tatacctgac tgcatcaggg ggaccttttt ataaaacaaa
    21961 aattaaaagt ccatctttaa aacaggttct ttctcatcct atatggaaaa tgggcaagaa
    22021 aataacaatt gattctgcaa caatgatgaa taaatgtttt gaaataattg aagcacatca
    22081 tcttttttct ttaccatttg aaaaaattgg ggttttattt catcctgagg caatagttca
    22141 tgggttagta gaatttattg atggaacaat aaaggcaatt atgtataaac cagatatgaa
    22201 aattccaata aattatgttt taaactatcc tgaaagagaa ggagaatctc ttgaaattct
    22261 accattagat aagatgaaaa aacttacttt tagagctcct tctggaaaag aaaaatttat
    22321 aaagtttgca aaagagtcaa taaaaataaa tggctcttat cctgttgttt taaatactgc
    22381 aaacgaagtg tctgttaggt attttcttga aaataaaata aaatttcaag atattttagg
    22441 gactattgaa aaaataatta aaaaacaaaa aatacaaaaa aatataacta taaatgatat
    22501 ttacagaatt acagaagaaa taaaaaattc ttattaaaaa aatgggaaaa atttattttc
    22561 cacagtcaaa aataggtaaa aaaggttttc caatagagac tgaattgaca attcttgata
    22621 ttgctcaaag aaataatatt caattatctg ctgaatgtgg tggacaagga aaatgcggga
    22681 aatgtgttgt aagaattgag aaggggaaaa gaaatttatc accactaaat caaattgaag
    22741 aaaaattttt cttatcagat aatgagcggc ttgcctgcca gacaaaaata ataaaagata
    22801 atggagatat agttgtctac ataaaagatt ctggaaggta tgaaatttta aagtttggaa
    22861 tggagagaaa aattcctatt tgtcctcgtt tttacaaaaa aaatgaaaat gtctataaag
    22921 aaggagagat tatagatgtt tatagaggaa atatttttgg attggcaatt gatgttggta
    22981 caacaacagt tgtttttgat attgtgaacc ttgaaaatgg taatattata gcaacagttg
    23041 ctaaaacaaa tcctcaaatt tcttttggta atgatgttat ttcaagaatt gaatatgttt
    23101 ctattgataa aaatcaaaaa agatatttaa atgatgaaga aaaaagagaa agagtaaaaa
    23161 aactacaaag agttattatt gaggcaatca acaattcaat tgatgaaatt tcgggaaaat
    23221 atggagaaaa tttttcagaa aaaatttata gtgttgttgt atcaggaaat cctacaatga
    23281 gaaatttatt ttttgggatt gatgtctctt cccttggagt aagtccatat gagccagtcc
    23341 ataaggaaga aataattgtt atgccagaag aaattggatt aaaaataaac aaaaatggag
    23401 atgtttatgg gattcctctt attggtggac atgttggagg agatattgtt gcagatattt
    23461 tatcatcaga aatttataaa aatgaagaaa tatccattat agttgatata ggaacaaatg
    23521 gagaagtagt tattggaaat aaatataaaa ttatttctac ttccgcagca gcaggcggtg
    23581 cttttgaagg tgggaatatt tcctttggag ttggagcaat agaaggggca gtgaaaaaaa
    23641 tagaaataat tgatggaaga gttttttatt ctacaatagg aaataaatat ccaattggta
    23701 tatgtggttc tggttttatt gatttacttg cagaactttt aaaatataat attttaagta
    23761 aaaatgcaaa attaaaacag gacttttaca taacaggaga aataaaaatt acacaggcag
    23821 atatgtacca acttataact tcaaaatcag caataaaaac agcacaggat atacttatga
    23881 aatgttatcc agttgaaaaa gaaaatttag ggaaaattta catttctggc ggatttggga
    23941 attttataaa tgtaaaaaat gcaatgaaaa taggacttat tccagaaatt gaagaagaaa
    24001 aagtcattaa aattggaaat ggttctcttg aaggggcaag agaaattttg ttatgtgagg
    24061 aaagaaaaaa aactgctgaa gaaattgcaa gaagcgtagt tcatataaaa acaaatgaag
    24121 tagaaaagaa ttttgattat atactggcag aaaacatgta tttttaacaa agagaggtta
    24181 tgaactcaag agaaaggata ttaaatgttt ttgaaggtag aaaagttgac agaatcccta
    24241 tttctttata tgaatttgat ggtaattatg atagttggat ttataattat cctgaatatg
    24301 ttgagattct gaattatgca aaagggaaaa cagataagat gtatttctgg tttccaataa
    24361 taaatagtcc atctattttt tatggacaaa tagacctaaa tgatataaaa acagaagaat
    24421 gggaagaaaa tgaccaattt catacaaaaa caacaataag aacacctctt ggggaagttt
    24481 atactcactc aagacaggat aaagggattc atacatcctg gacaattgag aatttatgta
    24541 agaatataaa tgatgctaaa aaaattcttt ctcttccata catccctatg aaagttgatg
    24601 tttcatcttt ttttgaaaaa gacaaaaaat taggagaaga aggaatttta atgggtgata
    24661 ttcctgatgc tttatgtttg gtggttagcc tttttggttt tagtagattt ttaatgtttt
    24721 atattgacaa cccggatatt atttttaaac tcataaattt ttttcaggaa agaatttata
    24781 attacttaaa agaactttta gaaaatggag caataacagt atatagaata tgtggtccag
    24841 aatatgctac tcctccttat ttaagcccaa atgaatttga taaacttgta actccttatg
    24901 ataagcaatt gttccaactt cttcacaaat atggagcaaa agggagatta cattcacacg
    24961 ggaaaataaa aaatgtttta aaggcatttt tagagataga aatagatgct actgacccta
    25021 ttgaaccact gccagatgga gatataacat taaaagaagc aagaaaaatt cttggagaaa
    25081 aaattgtttt gataggaaat atagaagaaa gattattgga aattggaagt aaaaaagata
    25141 ttgaagatgc agttaaaaaa gcaattaatg aagttggaaa tacaccattt attttatgtc
    25201 caacagcaat gccattaaca actccacttg ataaaaaaat aaaagaaaac attattcatt
    25261 atattgactg cggagggaaa tatgggaaat tataaaattg aaaaaatttt attgagtgaa
    25321 acttttttgt tatcattcaa tcaaaatata aaaggaggga gaaaatgatt gagaatataa
    25381 agtggttagg gcattcatct gttttaatag agaaagaagg taaatatatt tatgttgacc
    25441 catggaaaat aaaaaaagat gaaccaaaag gtgatttagt tttaataaca catccacatt
    25501 atgaccattg tagtccagaa gatatagata aaataaggaa agaccaaaca attattatag
    25561 gaccatctga tgcagttgaa acaataaatg gaaatacaag aaagataaaa ccgtatgagg
    25621 aaattgaact gccttatgta aaaataaaag gtgttcctgc atataatata aataaaaact
    25681 tccatccaaa aagcaataat tgggttggat atataataaa atttcctgat agttcaattt
    25741 atattacagg tgatactgac tttatacctg aaatgaaagg agttaaggtt gatattgtcc
    25801 ttttgccaat tgggggaaca tatacaatgg atgcagaaca ggcagcagaa gcaatcaaca
    25861 atatgaatgt aaaagttgca ataccaattc attatgggaa tattgtaggt agtaaaaaag
    25921 atgctgaaaa attaacttct cttgtaaaaa atgcagaagt aaaaattctt taaaaggaaa
    25981 cctcttttcc actttcagca cttatataaa ttccttcaag catttttatt acctctaagg
    26041 attgctctaa ttttacaggt acctctttat tttcctttac acattcaaaa aactccttta
    26101 tttcctgatg atgtggtggc tgaatatctg acatcacatt atcaaataat ggttttatat
    26161 cctgtaaaat accatttttt tctgttgcaa tttttccatc aggccataaa aatccacttt
    26221 ctgttccaaa taaagttgta gaaaaatcct ccaatttttc catatttgca agaaaactac
    26281 atgttaagac caaacttctt ccatcatcaa accttataaa tccagaagca aagtcctcta
    26341 cattatatgt ctctctatcc cactcacccc ataatcctct tatatctttt ctctttgcaa
    26401 gttttgtata tgtaattcct tttactgaat ggacttttgg gcatccgata aaccagtaag
    26461 tcaaatcaag aatatgaact ccgatatcaa acataggacc gccgcctgaa agttcttttt
    26521 taataaatgt atctcgggct ggtattcctc ttcttcttaa tgcattggca aaagcaaaat
    26581 aaatttctcc aaaaaagcca tcatcaatta gtttttttgt caaaacactt tcttttctaa
    26641 atctttgatg ttgtgctgcc ataagttttt tatttgattt ttttgcctgt tctatcatac
    26701 cttccacttc ttttacactt gtagcaagag gtttttcaca tattacatgt tttcctgctt
    26761 tcaatgaatc aattactgct ggataatgca gtttatttgg agtacataca tcaacaatat
    26821 caatttcctg aatctcaagc agttttttat aattatcaaa aatatatttt attccttctt
    26881 tttctgcttg ttttcttgct ttttctgaaa catctgctat cccaattact tcaacatctg
    26941 ataacttttt ccagccagga aaatgagttg aacttgcaat accaccaaaa ccaataaatc
    27001 caacacaata cttcccattt ttcatttttt ctcctttcct ttaagagtca aaaccaattc
    27061 ttacatcaac tgcaattact tctttttcct taacaattaa gggatttata tccatttctt
    27121 ttatttctgg aaaatcacta accaattgtg atacccttaa aagtgtttct ttgatttttt
    27181 ctttatcaac tggtggaata tttctgaaac cattaaataa tttacttgcc tttatatcat
    27241 caatcatatc agaaacttca ttttccataa gcggcacaag agaaaaagaa acatctttaa
    27301 aaagttcaac ataaattcca ccaagcccaa acattattaa atgcccaaat tgagcatctt
    27361 tattaactcc aactattgtt tcaattccac cttctatata ttgttgtatc tgtattcctt
    27421 caattttagc atctttttta tattctttca ctgaccttat tatgtcatta aatgctttct
    27481 caaactcaga ttctgttttt atccctactt ttactcctcc aacttctgtt ttatgaatta
    27541 tatcaggaga aacaagtttc ataactactg ggtatcccat ttttttaaag atatttttcc
    27601 cgtcttctaa atttttagca ataagagaat tgattgtatt tataccatag agagaaagaa
    27661 tttctcgtcc ttcaatatct cctacaactt ttatttttgc ctttttgaaa ttatccaatt
    27721 tttcttttac cttttcttta tttactttgt aagttataat tttttcttct ttcttttcca
    27781 aatattttct gtaatttacc atccctttaa gagaagatac agccctttca ggaaaaggat
    27841 aatttggaat ttttccctca cttaatctta taattcctgg ctctattctt tttcctccca
    27901 taaaacatcc tacaactgtc ttgttaaaac cttttgagac atcaataatt ttatctgctg
    27961 tttcttttgg ttcagttaca acctgtggag tgaggataca tataactgcc tctacatttt
    28021 tatcagatat aacaacatta agtgcttttc cataattttc tgccattaca tcacctaaac
    28081 agtcaactgg attatttata ttagaagatg ggggtagaaa ttcctttaac ttttcttttg
    28141 ttgtatctga aatttgtgca agtttaagtc catttttttc tattgcatca acagccatta
    28201 cagaaggacc tcctgcatta gttacaactg caattctatc cccctttata agtggttgat
    28261 atgaaaaagc aattgcgtaa tcaaaaagtt cttcaactga atatgctctt ataacaccac
    28321 tttttttgaa agatgcttca aaagcaatat cagagccagc aatacttcct gtatgagaac
    28381 taactgcttt acttcccatt tcagttattc cggatttaac aattataatt ggtttctttt
    28441 ttgagacatc tcttgcaact tcaataaatt ttctgccttc ttctatccct tctaaataaa
    28501 gtaaaataac atttgtgtcg gggtcatcag atagctcttc aataaggtca atttctgaaa
    28561 tgtctgcttt attcccgaaa cttacaaatt tagaaagtcc aatattttct tttattgccc
    28621 agtcaagaac tgctgttcca agagcgcctg attgagttat aaaggcgatt ttcccctttt
    28681 gtggcatatc aaaagcgaaa gaagcattaa gatcatttgt agtatctata atgcctaaac
    28741 aatttggtcc aataaggcgc atattgtatt ttttaataat tttttctaat tcttcctctc
    28801 tttttctccc ctcttctcct gcttctttaa aaccagcact aattacaatt aatcccttaa
    28861 cgcctttttc tccacattct tttgctacat cacagacgaa ttttgcagga acaacaatta
    28921 ctacaagttc aactttttct tttatttcag aaatgctttt ataacattcc agtccaagaa
    28981 ttttacctga ttttggattt acaggaaaaa ttttacctct gtacccccca tcaattatat
    29041 tttttaatat tttataacca agtttttctg gatttgttga aacaccaacc agagcaatac
    29101 tttcgggcat aaaaaattgt tttaacataa taacccccat tattaagcat tacttaattt
    29161 tttttgttat acttaatttt atttatttta gaaattatat ctttttaata gaggaaagtc
    29221 aaattttcga attgagacat aataaatcta ctccaaaagg tatgttagac aagacaaaaa
    29281 agaatctact ggaaaaatag gatttaaata gcaccttcgg ataaaataag aaaaaaccgg
    29341 aggtgcaaat gaaaaaggaa ttaaaaatgg gaataaggaa gaaaataagt aaaaaaatag
    29401 catagaggta tcagagaggg aggaagaaaa agaaagagaa atggaaatag aaaaagaaga
    29461 gaaaaataaa caatgtgttg gagtagattt ttatttgaca caacagtctc tgttcaaatc
    29521 gctatttaaa attgctgatg aatatgattt tggattaaaa taacctttta cacctggaaa
    29581 tgaagtaatt tcccactttt taatttttct attacgaact atcaggaaat atactttatt
    29641 tccttcaata cctacttctt ttttaggtat tctgaaatta attacccatc tatctggaaa
    29701 tttctttgtt tctgtttgat aattaccttg ccatgaccaa ttccatctat tagttaataa
    29761 atcacattca gaagcacatt taaaattatc tggatttata agaaaatgat aacaggtttt
    29821 tgcatcctgt gaagaagcaa taaaaatttc tatacaatcg cttccatata ctgctcctac
    29881 atcatttcct ttgtgaattg tcaaattttc catttcttct tcttggcatt caattataaa
    29941 tacaatttct ttattgtctg aatatacttt tacaagtgtc tggttttttt ctggtggaat
    30001 aagtgttatt ttatcaacta aaactaatgt tgaaggatct ttttggagag gagtttcaat
    30061 gttcatctga gaaagaggga aactttttat ttcactttgt gagtgatatt tttctctttc
    30121 ttcaaaaata tgtatccagt aatcaaaaag ttctttttct ttgttaactc gttgataata
    30181 ttctgggaaa ttcttacttt cctttaatgc attttctata ttaccaaggc attctttttg
    30241 gatttccttt gtgaacatct tcttaaaatc atctgttttc tttgtaaaac atccatccat
    30301 ttcttttagt cctttttcta aacagtccag ataacgacgc attgaagaat cagcaggacc
    30361 aaaatttttc tgacaataat catttaaagt attatctaaa ttagtattaa tattccagag
    30421 atacatcgct gttacataaa taggaagatg aaatgtttcc catgctttac tttcaactcc
    30481 tggtacttca cttaaaaaac catccaatcc caattgatgg taatatttta tatcctgggc
    30541 aactacctgc cagataggag caggtaaaaa atcccagtta taagtataat agtcataaat
    30601 atatatgtgt ttaattttcc ttcttaattg ttttatccgc tcaaaaattg gcttgtttat
    30661 aggacaatta gggtctccaa tagagtgttt ataacatctc caaatagtgc aagaaacgat
    30721 aagattatca ggtaaaacat cttttacttg ataaaagtca gaaaagttac tataagaata
    30781 atgtccaact aaaacatcag gatatacttc ttttacctta tttgcaatat cattaataaa
    30841 atcagcaact cttccattta cagaaccctt ttttctatct tcctgtgtat ctaaattttt
    30901 acagttttca cattcacacc agccataacc atcattagga ggaataccaa cgatagagat
    30961 atccggatgt tctttaatat attttaaaat attctggata aactctgtcc tcatctgctg
    31021 attagaaaga cataattgtc ctccttctga ctgttttact ctttttcctc ctattaaagc
    31081 aaaatattct ggatgttgtt caaaatatct attatctggt atccagaaac tataactatg
    31141 aacggtcgta tctggtttta aaccccattt tggtaattct tctttaagaa tatctacttc
    31201 gttatgatga gtaagtttac tattcatttt taaatgaaac atccattctg ctactttttc
    31261 atcataatga tgcttaccgg cacaaatatg tagacatcta taaggaaaag aaggtgagaa
    31321 tgtttgattt gtttcatcta attttattgg taagataatt tcttccaaag caggagagtc
    31381 aggacgaaaa tatctcactc caaataattc taaaagataa attattccac atctaattcc
    31441 ttcctcatca catcctgaaa tttttatttc tcttttggaa ggagagatat tcagacaaaa
    31501 agaaccttct tgaactattc ctttttctgg ataagaaaga ataatattta aatcttcagg
    31561 tgagagactg ttaacttctt caagtccaca ttttttaaaa aatgttttta attctaatat
    31621 agcagtttct gtctgtatta ttccattacc agaaggaaaa aaaatattta ttttatattt
    31681 atcattttta cctattttca tttgttcttt gacactacct aaaaaaaaac agggtcataa
    31741 acttttatta atgaaatatc atcaaaccag atacattcat cttgtttctg aacaggagca
    31801 ccaaaaagca aaacacagga agtaatatct tcaggagcaa taaaaaagaa ttcaaccttt
    31861 tgccatttac tttctctttc tttaggatag gagattgaaa ttcttggtgc ttttcctttt
    31921 ccctggtccg cccatccatt tttatccatc caccttattg tcattgaacc ccctttatca
    31981 ggggggtcct gtttaaccca gaaacttaat aaatatcctg cttttggagt aactttaata
    32041 cctgcctgaa aacatcctga tatctggttt tctctaattg aaattgaata tttccccgta
    32101 tgtgcttgtg ttgggtccca ataaaatctt gtgactgatt gttgaaaatt ccatgttgtc
    32161 caccctttta atcttttatc taatttcttc caatcaaatt tctctaattc ttcttcagaa
    32221 aaaacttctt cttgctttgt ttcctctcct ggctcaaaac ttccattgag aactaaattc
    32281 tgtttactct caggagattg cttgataaat atctgtgaaa gtgcaatact ttttatttca
    32341 ttatctttat tttcctctgc tacttttttc caaaatgatt gtatatcttc ctggctattt
    32401 ccttttttct caaaatactc cgttattttt gtaaatattt tatatgtttc attttcaaaa
    32461 tatggtttta taattaaagt ttgttgactt ttaaaacttt caaggtttgt atttacaaaa
    32521 atattttttt cttcctcagt ggtcatttga tagttctcta ttgattcaat tgctttaaac
    32581 cccatttttg ctttatttat aatatcttct attttttcat cttttttaat ctcttctata
    32641 ttctgcatat ctttcattgt aagataactc tccaaataaa attcacttaa cctccatatt
    32701 ttttcaaata aatctatcct tttttttact atttcatctt ttaataattc ttttgctttg
    32761 ttaagtttat tattaagata ttcaaggtca tcccagatat attcatcaag ttgtttcaaa
    32821 ctttgccaat cagctattgg atgtaatggg tctttttttc taccataaat ttcttcaatt
    32881 ctgtcaaaaa attctctcat cggtttattt gcttctccat aaaaatttaa aaaataatca
    32941 tctaaaagtt tatcaatatc agcatctata tcccatgcaa gttcatttaa aacatactga
    33001 cgaggtccat catataacca aacttgcgta aatgtctcaa accatccacc ttttaaattt
    33061 acacttttat atgcttcttt ccatgctttt cccattatat ggggataatg acgaggaact
    33121 atatagccac ccccataaag ataatcgtat atcccgatgt tttttgctcc tgcattgctc
    33181 catccctcta ataatttcat attttctctt aatccactcg ctacttctac ataaatattc
    33241 ggttctaatt ttatattttc aggaactgga gaggcgcctc cataagcaat aaaacttacc
    33301 aatttatctg gaaattgttt ctgcgctaat tttgctattt gattgtaaaa aggaatatat
    33361 tgatttttaa atttcctttt tagttctaaa caatctggac attgacaatc tcctccacca
    33421 tcatttacac ctactggaat accgagcatt tgtggatttt tttcaaagta ttcgttagac
    33481 cattcaattg caatttctgg taaatcagga ttactaacac atggctgcca ggtcccacct
    33541 gaattttttg gagggataaa tcttttccct tctatcattg gataatattc tggatgactt
    33601 tcagaatatt tttcaggtga ataaatatga taaagattat gagatgctaa aaatgttgtt
    33661 ctccatgaac gagaaaaatt cccatttcct ccgtaaaaac cggcatttgt atatgtaata
    33721 aaagatggtt cttccttaat atctattttt aatggtaaat ttattatttc ttttttagaa
    33781 attatttctc ctaattcccc tggcataaaa tacctatatc cacaaaacct ctttaaaaaa
    33841 tcataaatag catatgatgt ccctcccaaa tacttacctg caattaccaa actatctgga
    33901 ttgcttatta tataatatcc atatggatga agtttatcaa aatcaagtcc tatttcattt
    33961 acataattag ttcttccaac atatatcttg aaattttctt ttatatcttc tgctttttct
    34021 gtttctaata taactttaat attcccagaa gtaattttat ttagataatt acataaagtc
    34081 cttgcaatag atatacttga agaatctgat ataattataa cttctttctt tttacctgcc
    34141 acagatatga tttctgtttt ctgcccttta ctacttttta ttatttcttc tataggtggc
    34201 tcgtttttta atattgaaga aacaattttt ttaaccatta aaagtaaact actgctttcc
    34261 gggtcatctt tccaatcttg tttgtaatat gacataacta ctggtgaaaa atatataaaa
    34321 gtcccttttc cgtattgatt gataaaaact ttcccaatgc tctctgttcc tattattact
    34381 ctcccagctg taatatctgt caaacccggc tctgacccaa accaactata ctcttgtttc
    34441 gcaaaatgat ttacaatcgg attctcagga gaaataattt tagcagacac tttcccatat
    34501 tgataagttg ttgctcctat ccaatctgcc catgaagaaa attttctgtt gtctgatgga
    34561 acccaattac caatagttat tgttgttatt aaaatatgac caccttcttt tagatatttt
    34621 ttaactactt cattatcttc ctctgttaac ggttttggtc tatcctgact taatataata
    34681 aatttgtaat tcttaaattc ttttactgga atataaccat cttcttcttt taaaatatct
    34741 gactttactt cacactcttt ctccaaaaat tctttccatg tttttgaacg ccagccacaa
    34801 atacctattt ctgattcagc catcaaaaaa gtcggcaaaa ataaaaacac taataagcct
    34861 aaaatagttt ttgctctttt catatatacc ttgtatgatg aagttagatt ttttagagga
    34921 gttatttata gtaattatac ttcttgtgaa ctctattact tcacatacca taatcacctc
    34981 ctaattctct aactttatta taaaccctaa aaaaattttt ttcaagagac cggcatttct
    35041 caaaaataat tctactaaaa tatcctatac aaaagcagca cttttgtttt aattttttct
    35101 ccctttgtta tacacctaaa aaaatgtata atgtaaatta tgaataataa aaatagttta
    35161 gcgattgtta gaagacgaga aattaaagaa gtgtatattt ccgaaggatc tctcgggtgt
    35221 agaccctacg ggcgagcatg tggaaatgtc acatttttat ttggtaaaaa atgtcacata
    35281 atcatgtggt aatatatctt gttgacaaaa aaaaatttat gtgttttaat attaaaaata
    35341 atatagggag aatatgaaag ggggtgaaga caatggtgat aaagaaaaaa gccgcaagta
    35401 aaaaaggttg tagtaaaaaa acaacaaaga aaacaacagg taaaaaagtg gctaagaaga
    35461 aaaaatgaga agaaaaaaaa gaaaattgag atggcgttct aaaaaagcaa atcatagaaa
    35521 gaaaccaaca atgggataaa aaataggaat ggaaagcaat tgaacttaaa taaataatga
    35581 actaattgat gtttctaagt ggattctttt aattgcttct ccaagtaaag aagacacact
    35641 taaaatttta aatttaggtg gtaaagataa gtgtggtatt gtgttggtta ttataatttc
    35701 ttttaagttt gagttattta atttttctat tgtatcttct gaaaaaaccg catgtgaagc
    35761 acatccgtaa acctcaatag caccaccttt aatcaatatg tctgctgctt caattattgt
    35821 attacctgtt gaaatcatat catctacaat aattactttt ttacctttta cttctcctat
    35881 aacatgagtt acctcaactt tatcaggact ttctcttctt ttatccacaa ttgccaatga
    35941 agcatctaat ctttttgcaa aagcccttgc catttttact cctccaacat cgggagcaac
    36001 aacaaccaac ttatcaaata ctttttcttc taaataacta attacaatag gagcagcaaa
    36061 aagatggtca acaggtatat caaaaaatcc ctgtatttgt ggagcatgta aatccatagt
    36121 aagaattcta ttagcacctg ccgatacaat aagatttgca ataagttttg ctgttatggg
    36181 aactctcggt tggtctttcc tatcctgtct tgcatatcca aaatatggta aaacagcagt
    36241 aattctttta gaagatgctc ttcttaaagc atcaagaata ataagcagtt ccataagatt
    36301 ttcatttaca ggtggacagg ttgattggat tacaaaaaca tcttttcctc ttacattttc
    36361 ctcaattttt acacaaattt ctccatcttt aaatctataa atgttaattt tccctatttc
    36421 tttattcaga tatttacata tattttcggc taaattttta tttgaatttc ctgaaaaaat
    36481 taaacaactg tccatttttc actccttttt aaaagttttt ctgctttttg gagttcctct
    36541 attgtattta tacccatcac ttcctcaggt gtatatattg taaaagaacg gatttttact
    36601 ccctgacaag caagaatttt aataacatca gtaagataat attccccttt aacagggtct
    36661 tttttgagtt tttctaatgt atcaaaaaga agttgttttt taaatatgta tatcccagaa
    36721 ttgatttctt ttatttcttt ttgttgggga gtagcattaa cttcttcaac tatcccggta
    36781 attttccctt ctttatttct tataattctt ccataaccag ttggattatc aatagaaact
    36841 gtaattatag aacacattgc tttgctattt tcaaaatcat ttataaaatt ttttaatgta
    36901 tcataagaaa taaatggaac atcaccatat agaataagaa tattcccatc aaaattttct
    36961 aaaaggggca ttgctttctt aacagcatct cctgttccaa gaggaacaac ttgttcagca
    37021 aattttacat tttctccttc aagttgtttt ataacctctt cttttttaaa gccaacaact
    37081 attataactt catctggaaa aagttttttt actgtttcca taagataaga aagcattggt
    37141 ttgcctaata tattctttac aactttttgt ttttcaccgc ccaacctttt tccagaacca
    37201 gcagcaagta ttaccactgc aagatttgaa gccatttggt atctccttga actgcccggc
    37261 gaggatttga acctcgaaac tcggctccaa aggccgatgt gttaccatta caccaccggg
    37321 caaaaattaa ataacatata gtgaaaaccg ttatataaac aaaaaattaa aaatatgtaa
    37381 aacgaaaatg taaaatttaa gacaaagaaa ataattttaa agtttaaaac tctaaacgag
    37441 tattttatat ccaccaaaag agtgccaaat tcctgaaaaa gcactttaca atttcaattt
    37501 tacattatta agtttgaatt ttaacttcta ttgttttttg tcggatttgc cttttccttc
    37561 tcatattctt caagcacttt cttctggata taatctctaa actcgctttt tattggatga
    37621 gcaaggtctt tatgttgtcg ggaaggtgaa agacgctgtt cctcaacagg atttaaggca
    37681 gaaccacaag aaccacaaaa cttattacct acgggatttt taaatccaca tttaggacat
    37741 ggcctctgaa ttttcttact tggcattgca acaaaaagcc cttttttacc ctcaataact
    37801 cttatatctc ttattacaaa ttggttatca aaggtaacag aggcatatgc tcttaatcta
    37861 ctactcggat ttgtaacgag cgaaattctg gtttcggtta tttccattta tttcctcctt
    37921 tatctttgag ttgttaaagc attcaattat aaaaaaatcc cagccaacag caacatcacc
    37981 taattttatt gtttctgcct ctcttttctc tctaaaaata gaaaataaac ttccaccact
    38041 tcccgagaga aaaaattttc ttcccgacat tttttcaatt tctttcatca cctcctttcc
    38101 ttcttcccat atttcaaaac agggcttttc taatctgtta tacattatgc tttctgcttt
    38161 ttcaaattct ctttctctta ataagccgac aagaatttta atttttttta atccatttgt
    38221 caaattacca aattcccttt ttttatctaa ttgttcgtac acatccttag ttgatattct
    38281 gataggtgga acaagtaata agaaataata agttggaata taatttttca ctggctttac
    38341 aatctcacca tatccactta ctattgataa atttttataa ataaagaaag gaacatctga
    38401 accaacatta agagcaattt ttagcatctc tttttctgaa atatccaaat tgaacatttg
    38461 aataacattt tcaagaacaa cagcagcgtt tgaactacca ccaccaagtc cactaccagg
    38521 aggtattctt ttccatatat ctattttaac tggaatgtct gttttgaatt ttgtttccaa
    38581 taaatccaat gtttttttaa ctgtattatc aacaggaatt tcccatttag aatggaaaat
    38641 aatttctgtt ttttttgatt tttcaatttc tacaatatct gaaagagaaa taggggtaat
    38701 taaagaaaca ataggatgaa acttttcttt cttttctcct acttcaagaa ataaattaat
    38761 ttttgccggt gcaagaactt tcatctgttt ttggtttttt tgcaactttt acaagtgatg
    38821 gctttaaaat tttgtcgtga taaacatagc caggttgttc ttcctttaca atttccccat
    38881 cttcaaattc atttgtttct acaacgccaa ttgcttcatg tatatgcggg tcaaattttt
    38941 taccttttgt cactattttt ctaacaccat tttcctcaag aaattttgta aattcctttt
    39001 ttaaaagttc aactccttta tgaattgctt cattagatgg atttgtttcc atttgtttta
    39061 agatattttc aaatatattg tcaaattgaa gtaattgtct caaaagattt tcagccccat
    39121 acttataaat ttcctgtttt tctttttcaa ctctttttct ataattatca aactcagcca
    39181 gaactcgtaa atatctatcc tttaattcgt caatatcttt ttttaatttc tcattttctt
    39241 ttttaatttc ctttgttttt tttaacgatt ctttcaattt catcaaatcc ttctctttta
    39301 tatatactat tccttctttc ttattttcca ttttacatac acctcttatt ataatataaa
    39361 atgataacta aaaatgtaaa aagtttttat cttttgtttt gcttcatttt gaatccgcca
    39421 cactacatag tcggatgctc taaaatacgc attttacatt tttgttttac acttttccaa
    39481 tatttattct accttataaa acccattctc catggtggcc aatatataaa aagggctttt
    39541 cccaccaggt ccttttctgc tacaaatccc caatatctac tgtcactact gttaatacta
    39601 ttatccccga gtacataata tgaccctgga ggaacttctg tttctttcgc aaccccataa
    39661 tatccatcac tataataata cctgcaacta atcctcggtt ctgttacttc cttgccattt
    39721 atatatattt taccttcttt tataagtact ctttcaccag gaagagcaat cgctcttttt
    39781 acataatctg ttgtatgagt ttctttaata tgtttaaaaa ttactacctc acctctttgg
    39841 ggttttctaa ttttataaat tatatcattt gatataagtc ggtcaccaac cattaaagta
    39901 gggaccatag aagtagttgg aattttgaaa tttccaacaa aaaatattct aacaaaaaca
    39961 gcaataacaa tagcccagaa taacgattta aaccaatcag ttgtttcttt ttttatatct
    40021 tttttcattt tagtcatata ttttaccctt tgtctttatt catttcaata aattaatata
    40081 taattctgaa tatctttcta ccattttatc aatagtaaac tcctttttta ttttttcctt
    40141 tgcatttatt ccaattattt cttttaaatt tttatcttct aaaagttgct ttattttatt
    40201 aactaattct tcagaatttc tatcttttac cacaaatcca tctttcccat tttgaataat
    40261 ataaggtaca tctcctactg aaaaagaaac aacgggaatt ccaaaactca ttgcttcaag
    40321 gacacttaac ggacatcctt cccattgaga agtcaaaaca aaaatatcaa tagccgaata
    40381 taccctttca atttctttta caaaaccagc aaagacaaat ttatccttta ctccaaatac
    40441 ctctgtaaga tgttttaaat atactttaag cggtccttct ccggcaatta aaaatttcac
    40501 atcaggaaaa ttttctgaaa taactttaca tatttttata aaaatttcct gtccttttac
    40561 ttttcttaaa ttaccaacat taccaataac aatactattt tcactgaaac caaatgaact
    40621 ttttgaaaat gttctttttt ctgttgggta tttgacacca tttggaataa ctacaatttt
    40681 tttttcattt attttccctt tttcaacaag gtattttttc ccggaaaaag agtttgaaat
    40741 aataatggtt gataattttg aagttaatct atctattttc cagtgatacc attttctcca
    40801 tttatctaca cttctttgag aagaaataat aatttttata cctgcaatct tacctaaaat
    40861 tcttccaatt aaatttgaaa taaaaagaaa tgattgacag atatctggat ttattttttt
    40921 gattttttta tatacaataa aaatagaaag tggctctttt tttaaatcat aaatataagc
    40981 aaatatattt ctttttttaa tttcatctgt aaaagtacct tctcctctta aacaaatgat
    41041 gtatatatca aatttttttt tattcagatt atctataagt aaaagcaatg acttctcagt
    41101 tcctccaatt tctgaactat ttagcaggta taatattttg atttttttat cttccataat
    41161 atggttcaag aggaggtgac tttatacctc ttttgatttt ttcatatgca aggaaagcat
    41221 tataacttgc cagaggataa actggaaact taaaaacatc ttttttataa ttattcaaaa
    41281 aaatgttcac acctggacca ataggaatat aatcttcttt ttttaaaata tttttaacta
    41341 ttttttttga aaatgttttt attgggaatg atttcttaaa gttaaattta cagttatatt
    41401 ttgaaatata aaaattttct ctgtatgcat taacaacaca aataagtttg catctaaaat
    41461 ccttcaaaat aaaaaaaagt gaatcaggta atgaaacagc atatattttc tcttttttac
    41521 cacaggcaat cccttttaca aaagatatcc ctgtccttat accagtccag gaaccaggtc
    41581 ctattgagac aataaaataa tcaaaatcac ttgtttttat ctcacaatta atattttcaa
    41641 gattttctac caagcaactc gctatatcat cacttttaaa agtaatttct ttcaataaat
    41701 taaaattttc aacaactgct atactcccta ttgaagaagt tgtctcaatg gataataatt
    41761 tcattttaat caacagaatt taatattctg gatattaaag ccctatctaa tttctcctta
    41821 ggattgattt ttgcctttga aataagtgaa agtttggctg tacctaaggt aactctccct
    41881 tctaaataaa cagggactct ttctttatca gttgtaagcc atatattcat tttacctttt
    41941 ctaaaaactc cttcatcact ttctgctatt ggttcaagga ggtatatttc cttattccca
    42001 agaagaggta catcttttgt cattttatct tttcttgtaa ttgttaaatt ccagattttt
    42061 tccattgcaa caggatattg gaatttctgg taactctcaa aatcagcact cctcaaaaca
    42121 taaaaaataa acattccatc atataaattt ccattaaatt ttactttttg tgttttattt
    42181 gaataaacag cccctttttt taattttttc acattccagg tgtattcact cttattaaaa
    42241 ttaaaaaata tctctgtggt tctttccttt tccccatttt ttacataaga ataaaaataa
    42301 tatggtttta atgttttttt atctatgaaa gaatcaactc tattatatcc atatccactc
    42361 tttgccatac ttccttccgg gacagtaatc cccacaatat gatagaactc accattatta
    42421 tttttctcct ctttacaaat aattattccc ttcccaacat taacaaaatt ccattttgca
    42481 gaataaagaa gatattctcc tgaccatgaa gtaaaataat ttaacaattc aataaatttc
    42541 ccatcctgtt ttttttcttt ttcatcaggt attacaggat taggtaattt ctgctctttt
    42601 tttgcaataa gagacctttc atcttctaat ttcttttctt cttgtgataa atttatttct
    42661 ggctgaatta ttgtttcttc ttgcacattt tcttctatgg ttaaagaaat ttcatctgtt
    42721 gataaaggtg cttcttcagg tggataatta acatcaggga tttcttcaag agttacctct
    42781 ttgttttttt tgccagtaca tcctgccaaa aatacgaaaa tagccagata aataatgatt
    42841 tttttcattt ttttagacca ccctttatga ttttctatta taataacaca ttttggaaat
    42901 tttttaaata aaaattctca ataaaagaaa aatttaaagt caatatgctt tatatctcta
    42961 tctctgaata atttatacta tttttcttag atattaatgt ttttattttt tctggtaaat
    43021 aaccaaattt atcttctatt tcttcaataa acttactctt ttcttcttct gtttttattt
    43081 cacttaattt tttatatatg tagaatctaa atgatgggaa gggtgcaaaa tcatctggca
    43141 aaataaatgg aaattcaacc tcttctggtt gctttacttc ctgccctttt atttctccca
    43201 ttacttctct ccagaacctg caataaaggt taaatccaac catgtcaata aatccatgtt
    43261 gttgcttccc taaaatattt cctgctcctc taatttcaag gtcttttaaa gcaattttat
    43321 atccactacc aggactattt aatttctgta atgcatctat tctctttctt aaattttcgt
    43381 ttaatggaat atgtttcgga ataaaaaagt aagcataagc gcgccattta tatcttccta
    43441 ctctacctct caattgataa aggtcagaaa gtccatatct tgttatatca tcaactatta
    43501 aagtatttgc tcctggtata tcaagcccat tttcaacaat acttgttgca acaagaacat
    43561 ctatttctcc ctttgagaaa gacctcataa tttctgaaag tttctctggt tttaatttcc
    43621 catgagcaac ttcaacttta ctttcaggga caagtttctt aattttctct tttacttttt
    43681 caatatcata tacatagtta tgaagataaa atgtttgtcc tcctctttcc atttcttttt
    43741 ttattgcctg ctttactatt ccttcattat atattccagc atatgttata actgataatc
    43801 tcccttctgg tggtgtttca attaaactta tgtctttcaa tccactcaaa gacatataaa
    43861 gtgtccttgg aataggagtt gctgttaaag ttaaaacatc aacttttctg aacatcgctt
    43921 ttattttttc tttatgaaca acaccaaatt tttgctcttc atcaattatc agaagtccaa
    43981 tatctttaaa tttcacatcc tgctgtaaga gtcggtgtgt cccgataatt atatctattt
    44041 ttccttcttt caatttattt attatttctt tttgtttttt ttcagataca agacgggata
    44101 gttgctcaat tatcacaggg aaatcaacaa atctctcaga aaaagttaaa taatgctgaa
    44161 gacaaagaac agttgttgga actaaaacaa ctacctgttt tccacttaat acggatttga
    44221 aagcacacct cattgctact tctgtttttc catatccaga atcaccacaa ataagtcggt
    44281 cagttatttt ctcactttcc atatcttttt taacttcttc tattgctttt aattgatctt
    44341 ctgtttcttt ataaataaaa ctttcttcaa attcattttc cattttatcg ttagaaacaa
    44401 aattaaatcc tctttctttt tttctttcta cataaagggt ataaaggtct gatgcaaggt
    44461 ctctaattga atttttaact ttttccctta tgttaatcca atttttactg cctaattttg
    44521 ataattgtgg tttcttatct ccaacatatt tattaacaaa ctgtatttgt tcaatgggta
    44581 tataaagttt atctccacca tcatattcac ctacaataaa ttctttcttc tccccatcaa
    44641 attccatctt ttccattcca acaaattttc ctattccttc attatagtga acaatataat
    44701 cccctttttt aatttcttcc cattctgtaa tattttggaa attgcgtttt ttaaatgggg
    44761 tgtattttac tttatatctt ccaaaaattt catcattgct tatataagta atattttctt
    44821 ctggaattga gaaactgtat gataaattac cttttaaaaa ttcaatattt gatatatcaa
    44881 tatttctctc aataagtatt tctttaagac gctggttctg agattctgtt tgtgagaaaa
    44941 tacttatatt ttcaccagga taagtgttcc agataaattg agaactgatt ttaaacctat
    45001 caccagcagg aaaaacatta aaataaaata tatttttatt ccttaaaaaa gttaattttt
    45061 caccaactat atttgatttt tctattatat cctgaatttc tccatttttt tcaataaatt
    45121 tctctcctct aattatgaaa tttattccat taacctggga aataaaactc tcctgtcttt
    45181 caccaacaaa aaattcgctc agaggtaata tagaacattt ttcaattttc tcaaaagaac
    45241 tttgatttga aggattaaat tttcttattg aatttatacg atttccaata aactcaactc
    45301 ttaaagggaa atcagaaaaa ggaggataaa aatcaacaat tccacctctt gtactaaatg
    45361 ttccttgaat ttccacaact tcttctttat catatccaaa ttttacaaga atctctataa
    45421 aatcatctct tcttatattt tctccttttt caacttctat ttttttaaaa tttgagaaag
    45481 gaggaatttt atttaaaatt gcagggaaat atgtaattat aagtgacttt ttattctcta
    45541 ttatttcaat aatacttttt actctttcaa tattaacttc tgaaatttca tcttctactt
    45601 tttcatattc agggaaaaaa attacttctt tctcatctat aaaatttaaa atatcagaat
    45661 aaaatatcat tgcttctttt tcatcaggaa gaataacaag aaagtttgaa tttgaaaatt
    45721 tgaaaatatt tgatataaga aaagagaaaa aagagagatt gttccctaca atagaaaaaa
    45781 ggtctctttt ctcaattaac cccagtatgc tatttacctt ttccatcagt ttcatttctc
    45841 gtcctcttgc taaaattttc cagttgacca gatatttcca aaatcaagtg tctctttaaa
    45901 aattttgtaa gttgtttcat caaaaagaac aaatctaatt ttttttattg attttaactt
    45961 aggaatttcc tcaataacac ttttaataga aacaatcgct gcttcttttg ttggataacc
    46021 aaaaactcct gttgatattg caggaaaagc aattgacttt aaattttttc catcagcaat
    46081 ttttaaacaa tttttataac attcagcaag tttggcagca ggacctttct cctgtaagta
    46141 aacaggacca agagtatgta taacataaga atttggtaaa ttataaccaa atgttattac
    46201 tgcctcccct gtttttatag gagcaagttt cctacattct tcatagagtt ttggccctgc
    46261 ttttttatga attgcacctg caatacctcc cccaggagaa agatatgcat ttgcagcatt
    46321 aacaataaca tcaatatctg cctgttcagt aatatcccct cttataactt caatttcaac
    46381 atcacctatt ttcacaattc acctcctttg tacctttttt gacactaatt attattaaag
    46441 tttctgagta taaaagatac ttttaactaa tttttgaaac ccatgtttta tgtacatttt
    46501 ttgagcaggt atatttattt caagagttga aacaattgca gtctccatcc cctcttttct
    46561 catttcttca agaacttttt tatgaagaga agacccaata ccttttcctc gaaattctgg
    46621 taaaacacca ttacttccaa cttcaccaat tttcctctca aaatcaaggg tataagttga
    46681 ataccctata attttcccat taacttctcc aacaaacacc caatcaatat gacttttaca
    46741 gaaattttca acttctcttt ttttatattg ataccattct tttccagcaa ttttcccata
    46801 ttttttttca agaagttctg ctattgtata gccatcccat actttttcag tcattttcac
    46861 aatttcttca atatcttcac ttcttgcttt tcttattttt atttgcatat tcgcctcgcc
    46921 ttgttataat tattttacct tttattactt aaaaattcac ttgataaaaa attttaatta
    46981 tttactaaaa aactttatag gaggagaaga tagttatgat ttacagaaaa tttgggaaaa
    47041 ctggaattaa tgtttcaatt ctcggtttcg gtgctatgag attaccaatg attgaaaaaa
    47101 atggagaaaa acatgtaaat gaagaatatt caattgaaat tatacagaaa tcattttctt
    47161 atggggtaaa ttatattgat tctgcttatg gatattgttc aggagagagt gaaatagttg
    47221 ttggaaaagc actgaaaggg tggagagaaa aaatatatgt atcaacaaaa gttcctactt
    47281 ataatattga aaaaagagat gattatagaa gatttcttga ggaacaacta aaaaaattag
    47341 atgttgatta tattgatttt tatcatttcc acttacttga taatacaaaa tttgaagaaa
    47401 aagttattaa attaaaactt atagatgaag cagaaaaagc aaaagaagaa ggacttataa
    47461 aacatatatc tttttccttt cacgacaaac cagaaattat gaaaaagata attgatacaa
    47521 acttttttga aacagtttta tgccagtata atatccttga tagaagtaat gaagaggcaa
    47581 ttgaatatgc aagaaagaaa ggagttggag ttgcagttat gggccctgtt ggaggtggtc
    47641 gacttgaaaa agcagatgaa cttgttaaat tttttaaaaa taaatatcat tcaagtcctg
    47701 aaattgcttt aaaatttgtt ttttcaaatg aaaatgtctc aattgcttta tcaggaatga
    47761 atacaattga aatagtagaa gaaaattgta atattgcgtc aggtgggaaa tttcttgctc
    47821 aacaggataa aaaattactt gaaaaatgga tagaagaaag aaaaagaaaa aaagaaattc
    47881 catgtactaa ttgtaagtat tgtatgccat gcccttttgg tgtagcaatt ccagatatct
    47941 ttaatataat gaactattat aaaatttatg gactatttga ttatgcaaaa aaagcatata
    48001 acaatattgg aaaatgggaa aaagatgaga ggaaaaaagc aaatgcctgc gtggaatgtg
    48061 ggcaatgtga ggagaaatgc cctcagaaac ttgaaataag aaaattactt aaagaaatac
    48121 acaataccct ttttaattga cagatttacc atattatttc ataatataat tatgaagaag
    48181 ttgagataca taggtaacac ctgccagttt tttgaaaagt ttagggattt atataaaagc
    48241 aacaataaaa aaaggaggaa aaaatgaaat tttcaggaat aagtgatgaa gcaggacagt
    48301 caattgaaat gcagataaaa gcacataagg aattagggtg gaaatatatg gagttgagaa
    48361 atgttgatgg agaaaatttg acaatggtga gtgatgagaa atttgaagat gtttatagaa
    48421 aagttaatga aaatggtctt cttgtttcct gttttgcaag tggtattgca aactggtcaa
    48481 ctaaaatttc tggtgatttt actaaggatg ttgatgaact taaaagagca ataccaagaa
    48541 tgaaaaagtt caatacaaaa ttcataagag ttatgtcctg gcccaatgat aaagataatc
    48601 catgggaaga aaaaaaatgg ggagaagagg ttataaaaag aatgaaacaa ctttctaaaa
    48661 ttgcagaaga tggtggagta attcttgctc atgagaattg tagtggttgg ggaggtctat
    48721 ctcctgaaaa ttcaataaga ttattaaaag aggttagttc ccctgctttt gtcctcctgt
    48781 ttgatacagg aaatgttatt ccccatgcaa atatggatac atgggatttt tacattaaaa
    48841 ttaagccata tataaaatat gtccatgtta aggattataa agcagataag agtggagcga
    48901 ctttcccagg agagggctct tcaaaagtaa aagaaattct tactgacctt aaaaattcag
    48961 gatatgatgg gttcgtttca attgaaccac atattgcaag tatagttcat gaaggaaaag
    49021 ttggagaccc tgaattaact tacaggtcat atattgaata tggtagaaaa ttaatgcaac
    49081 ttattaaata aaataatttt tacactttta cttttcttgt ctctgtggat attaatttac
    49141 catctctgag atattgaatg gtaactgttc tttcatttcc ttcaagttta ccaggtgttg
    49201 caataatttc ttctgtaata ccattttctg ttgtccttga atattttaca gttgtattta
    49261 aagttgctgc ttcttttgct gcacgcgaaa caatatcttc ttttgtctct acaacattgt
    49321 tcttatccat tgaatctcca attaatgcac ctgctatagc accagtagca cctccaatga
    49381 tagcccctct ccatggttta tttgaatcta tgatagcacc taaaattcct ccactaattg
    49441 ctcctatccc tgctcccttt ttacctttct cagaagacca ttccacgctt gcacatccac
    49501 tcaataacat accagcaata agcaatacag caattcctct cttcattttt acctccttgt
    49561 gaaaatatgg tatcgtctct acaaatttat tctacttttt ctattacatt tgtcaacagt
    49621 tttctttctg tactcaaaca ggtaaaataa taataaaaaa tgaaattcat tgctgatggt
    49681 atgttaggag gtttagcaag gtggttgaga tttattggat atgatacatt atatttcaat
    49741 acatcacgga aaattgattt tatcagacag gcaaaaaaag aaggaagaat tgttctaaca
    49801 aaagatagaa aattatgtag ggactttcct ggacttgtat attttgtaaa tggagaaaat
    49861 acattagaac agtttaaaga tgttattaaa aattttaaaa tcagaataaa agaaaaggat
    49921 atattttctc tttgttcaat ctgtaataag aaacttgaaa gaatagaaaa agaaaaaata
    49981 aaagggcaga ttccagaata tatttatctc acaaaagaaa aattctcaat atgtcctgat
    50041 tgtggaagaa tttattggga tggagaccat tgtaagagaa taaaaaaagt aataaaagaa
    50101 atatgaaaaa aattattttg attttttctt tactttctat tgttcttatt tcctctccaa
    50161 aagaagcatt attttataaa aaaattgaag gtaattttgt tcagtgtttt ttatgcccaa
    50221 gaaaatgtgt tataaagaca ggtgaatatg gtttttgcag agcaagaaaa aacatagacg
    50281 ggactcttta ttcaatggga tattcaaacc catgttctgt tgcaatagac ccaatggaga
    50341 aaaaaccatt ttttcatttt ttgccaggaa cagatacatt ttctattgca tctgctggat
    50401 gttcattaag atgtaagttc tgccaaaact ggcaaatatc tcaatactct cccgaagaaa
    50461 caaaaaattt caatcttcca ccagaaaaag ttgttgaata tgctaaaaaa aataattgct
    50521 tatcaattgc ctatacttat tcagagccag tgaactttta tgaatatatg tttgatacat
    50581 caaaaattgc aaaacaaaat aaaattaaaa atattgtcca tacttctggt tttataaatg
    50641 cacaaccact tgaacaatta tgtccttata ttgatgcagt aaatgttgac cttaaaggat
    50701 ttaatgaaga atattataga aacatctgtc agggcgattt acagacaatt ttaaatacat
    50761 tgaaaatttt caaaaaacat aaaatatgga tagaaattac aaatcttatt ttgcctggat
    50821 ataatgataa accagaagaa attgagaaaa tgtgcatatg gataaaagaa aatttaggtg
    50881 attatgttcc tttgcatttt tcaaggtttt atcctatgta tcagatgaaa tttttacctt
    50941 caacccctgt ttctacatta gaagaagcat ataagattgc aaaaaaagtt ggcttgaaat
    51001 atgtctacat tggaaatgtt cctggaacaa aatatgaaaa tacatattgt ccatattgtg
    51061 ataaactttt aataaaaaga gttggttttt ctgttcttga aaataacatt gtaaatagta
    51121 agtgtaaata ttgtggtaaa aaaattgaag gtgtgtggga aaaatgaaaa aatttatttt
    51181 attttttgtt ttaattccat ttttacttta ttctgaaaat gtaagaaaac cgaattttgc
    51241 aggtagtttc tatcccgaaa atactgattc tttatcaaaa atgataaata attttctaaa
    51301 aaatgttgat aataagaaga taaaagtgga aaaagataaa attattggtg ttattgctcc
    51361 acatgctggc tatatttatt cagggcaggt agcggcatat tcatttaaaa cacttgaaga
    51421 tatgacaaat aaaacattta taattattgg gagaagtcat catgcctatt ttaaagaagt
    51481 tataattgaa gatagggatt tctggcagac acctcttgga aaagttgaaa tagataagtc
    51541 actttttgaa gaactttatg cccaaaaaca atttcatata aatcataatt tattggatat
    51601 tgaccatgca attgaagtag aaattccttt tctgcaaaaa gcatgtaaaa atttcaaaat
    51661 tttcccaatt ttgcttggag accttgataa tgaaaatata aataaaattt ctagcattat
    51721 ttacaatgtt attaaaaaaa gaaaggatat aatttttgtt gtgagttctg acctttctca
    51781 ttattaccca gaaaacattg cagaagaaaa agataattta ttatttaaat atcttaaaac
    51841 aaaggataca aatttaattt ataataaatt aaaggaaaga aaagaagttg aagcatgtgg
    51901 agatgctgca attcttttat tgatgaaaat agcagaaaaa attggttctt acagaataga
    51961 tgttttaagt cattcaaatt caggaaaaat tactggtgag aaagaaagag ttgttggtta
    52021 tggttcaata attattttaa aaagtgaaaa aaaggaggag aatatgctta caaaagaaca
    52081 aaaagaaaaa cttttaaaaa tagcaagaca aacacttgaa ttttatttaa aaaataagcc
    52141 attgcctgaa ataaaagaaa caacccctgt tttaaatgag aaaagaggtg tttttgttac
    52201 attaaagaaa agagggaatt tgagaggttg tattggttat atttatcctg ttgtacctct
    52261 tattgaaggg gtaagaaaaa tggcaattga gtcagcgaca ggagacccaa gattttcacc
    52321 tgtaacatat gaagaactac aagatgttga aatagaaatt tctgttttaa ctgtcccaaa
    52381 aagagtaaaa agttcagatg aaatagtttt aggtagagat ggagttatag ttaaaagagg
    52441 atttaatcag ggtgtttttt taccacaggt agcagatgaa acaggatgga gtaaagagga
    52501 atttttaagt caactttgct ggcagaaagc aggtctttct ccagatgcat ggaaagaccc
    52561 agaaacagaa ctttacactt tccaagcaga agttttcagt gaaagtgaat ttaaatgaaa
    52621 ttacatggta attttttcct ttcaggtatc atttttgggt tttccttttg tctcataaac
    52681 tgtgggcttt tactttttcc tgttgttgga gaaggaagtc ataactacaa acagggatta
    52741 ttgaaaggca ttctttttaa tttcggtaag atattttctt attcttttta tggtggaatt
    52801 gcctcttatt cgcattttct tttaaaaaca tttattgaaa gtaagtattc atttattgtc
    52861 ggtggtattt ttcttattat ttatggtata tggttcttct taaaaacagg tggaaaatat
    52921 gtagtggcag gaaaatttca aaaaataaat ttacatattt ttcttttagg actgatttat
    52981 ggcttagttc cctgtggtcc tttaattgga ttctttttat atgtttttta tgtcagtaaa
    53041 ggaatattat ttggagtgat ttctggattt ctttttggat taggagcaat ttttggacct
    53101 cttacccttt gtattttcat cccatatatc tggaaatttt tatatagaag ttcatttaag
    53161 atatttttaa gattgatttc gtcttttatt tttatattct ggggtgttaa cctaatttta
    53221 ttatctcttt ctcattagga aattaaattt cccaatataa ccgcgggggt taaattctct
    53281 aatatgtgaa gagactgttg tgtcaaataa aaatctactc gaaatactaa ttcgttgaga
    53341 cattaaaaat ctactccaac acattgttta tttttctctt ctttttctat ttccattt
//
LOCUS       DAOOVW010000004        51906 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_404_length_51906_cov_9.570577, whole genome shotgun sequence.
ACCESSION   DAOOVW010000004 DAOOVW010000000
VERSION     DAOOVW010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 51906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51906
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_404_length_51906_cov_9.570577"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..300)
                     /locus_tag="PLW95_00945"
     CDS             complement(115..300)
                     /locus_tag="PLW95_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21236.1"
                     /translation="MINGTLDDVRNYAKKLIYYFGKFNGGFIAKWYPSPQAIGHSWEK
                     IKVMVKTFIKYGSSFYR"
     gene            complement(325..534)
                     /locus_tag="PLW95_00950"
     CDS             complement(325..534)
                     /locus_tag="PLW95_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21237.1"
                     /translation="MSISGNKNNFKRDSKKAILFQSPNLHSCGYIVDILDGLIESKLN
                     VIQMDQQENMDVENLSNRFGGHICF"
     gene            complement(515..1420)
                     /locus_tag="PLW95_00955"
     CDS             complement(515..1420)
                     /locus_tag="PLW95_00955"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HOV21238.1"
                     /translation="MDIYSESGRIKALEKKFLTSKQVQSIIEAKNIGEVISILEKSFY
                     KFPSPILNTEEIIELLDKERNFIYEEIEKISPDILRIFLLKNDFFNLRIILENKSNYV
                     LPCNLPGQILKGYIEGNKGNIPDFLKKGVKIVKSEKNIEEKLLDLKNEYYIQLYNLLT
                     AFNSSFIEKYGKIEIDFANLSTFILKKKKGEKIVSSFFIRNGNTRAEKFLNEEAILKS
                     LTKEYKITDTFINEENFEIIRYKAIMNYIKEGKIIPYGIEVIFSYFIAREIEADIIQK
                     LLISKFYNIDENILKKQPVIVYQWK"
     gene            complement(1404..2021)
                     /locus_tag="PLW95_00960"
     CDS             complement(1404..2021)
                     /locus_tag="PLW95_00960"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011012532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOV21239.1"
                     /translation="MEKKKEFEELEEESRNCQRCKLYKGRTNVVFGKGSIDTRILFIG
                     EGPGFNEDKKGIPFCGKAGEILDLLLSSINLKRDDVYITNIVKCRPPNNRDPEDEEIK
                     SCSYFLSKQIELIKPEIIVPLGRHSMREIFNRFSIDETGAISKVHGNIYEKKGVFILP
                     LYHPAVAVYNPDRYLLLKKDFEKIRELIERKKYYNKEGEENGYLF"
     gene            complement(1999..2547)
                     /locus_tag="PLW95_00965"
     CDS             complement(1999..2547)
                     /locus_tag="PLW95_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21240.1"
                     /translation="MENLIKKLMEEAEEKRKKIIDEAQSVLNSEWEKKKFLIEKEYEE
                     KLKKMKETIDKEIENEIINFKIEKNKEILSFKNQLIDKCEEKIKNKFNEYLNKNIGEI
                     IFQILQTINDKKVKILVPENVNLKIDGYEVIKEKHLEDKFLIEGENSDIEFSWENIKK
                     TIGQELRKEVAEKLFNGKEKGI"
     gene            complement(2549..3022)
                     /locus_tag="PLW95_00970"
     CDS             complement(2549..3022)
                     /locus_tag="PLW95_00970"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOV21241.1"
                     /translation="MNLGLLYALIGAGLAIFLCGTGSSIGIGYSGSAANGVLSEEPEK
                     FGTMLLLVALPGTQGIYGFLTGFLIMMKVGILGGQIVTVSPSAGLQILLSCLPIAISG
                     LTSAIYQGRVCAAGISVASKHPEASMRAMVYAAMVETYAVLGLVASIFLLFVVKI"
     gene            complement(3025..4206)
                     /locus_tag="PLW95_00975"
     CDS             complement(3025..4206)
                     /locus_tag="PLW95_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOV21242.1"
                     /translation="MIKIIYEKCNLCKNCIESCPFGALEIKENKININEKCTLCGLCI
                     KNCPEQALYKEEKEIKIDTSDYKGIWFFAETREKGISSVSYEMLSACLKLKEKLNEEI
                     SGVLLGGDVSSFASQLIERGCDNVYIVENEKLQTFKEQTFADAFSKLIKKYKPSIVIA
                     AATMIGRSFIPFTAARIKTGLTADCTDIDIDEETGLLVQTRPTFGGNLMARIICKNHR
                     PQMATIRPKIFSPAEVIDGKEGKIIKENIEIDIEENVEVIGRERVENVVDLQEAEIII
                     SGGRGIKAAENFSLIRELAGLLGAAVGSSRAAVDSGWIPYPHQVGQTGKTVKPKIYIA
                     CGISGAIQHLAGMQTSDYIIAINKDPDAPIFKVANLGIVGDVFEILPLLIKKLKQIKG
                     G"
     gene            complement(4206..4991)
                     /locus_tag="PLW95_00980"
     CDS             complement(4206..4991)
                     /locus_tag="PLW95_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOV21243.1"
                     /translation="MRIVVCIKQVPESLDVKIDPETKRIVREGVKSIINPYDTYAIEE
                     GIRLKERFGGEVLAISMGPFQAEEALKEAIAMGVDDGFLLTDKKFGGSDTLATSFTLS
                     CGIKKTGNFDIIICGRETLDGSTGQVGPEIAEFLKIPYITFVSKIENISDNIIYCTRL
                     MEDHYEKVMAKLPVLITVVKEINEPRIPSLKGLLKAKKAEIKRITADQLGEDERFFGQ
                     DGSPTRVIDVWTPEIKKEGKKIEGEPEYLTDEIIKQLKTLGVV"
     gene            5116..5667
                     /locus_tag="PLW95_00985"
     CDS             5116..5667
                     /locus_tag="PLW95_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21244.1"
                     /translation="MEKKRKSFYNKFEIKKKGSIDENINKLQNELDIYLKGAEEIVEE
                     DEILEKKDIKKPEKKASFKKEESNEKYKESTYKHKDITANVNAKFNKKILFIPLFLFI
                     LFIGFIKFPLFKYTTTPKKDRDIPGIEIKTIFYYNVLGTKLKEKTILNVGNKEIIVPI
                     SIEKWFGLSKEEKIKYIKVFQSE"
     gene            complement(5737..5809)
                     /locus_tag="PLW95_00990"
     tRNA            complement(5737..5809)
                     /locus_tag="PLW95_00990"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5773..5775),aa:Glu,seq:ctc)
     gene            5855..6898
                     /locus_tag="PLW95_00995"
     CDS             5855..6898
                     /locus_tag="PLW95_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21245.1"
                     /translation="MSRYYKIAGCIHIHFPIKFFEKGIKLVAENANRAGLDFAILTSH
                     VPEKENKYKEILNFNSYYGKTLIISGQETNDIRKKNHLIIVGEKEWYRKKENIQEIIY
                     KVKNKNAFTIVAHPFGEHRLSLKKKNYEWENWENEFDCVEVWSLLFDWATFTNPFNLP
                     FRYFTFPDNLNGPDKRAVLMWDSISNKRKIPAIAGLDIHLLPPILRFFDIKKNFLYET
                     TFKILRNHLFLKENLSGNSLDDVKRIFECLKRGNLYFSNDYLKNSDGFYFGEENGDFL
                     MGDEGQFGKKIIIETPFKGEIKLIKNGKVIVRKNDKFLEYFVEEKGNYRVEVYFNNKP
                     WIFSNFLYFGSFC"
     gene            6975..8411
                     /gene="gltX"
                     /locus_tag="PLW95_01000"
     CDS             6975..8411
                     /gene="gltX"
                     /locus_tag="PLW95_01000"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOV21246.1"
                     /translation="MVRVRFAPSPTGYLHIGNARTALFNYLFARKNKGKFVLRIEDTD
                     KERSKREYEEGIIEDLKWVGIDWDEGPDIGGPFGPYRQSERLSIYRDIAEKLLKEGKA
                     YKCFCTKEEVEGRTKETGYDGKCRNLTKEEIEKLEKERKEYVLRFKVPEKKKVKVNDL
                     IRGEVEFETDFITDFIIMKPDNTPTFHFAVVVDDVLMEITHVIRGEDHLSNTPKHIML
                     FEAVGGKIPQFAHMSMTLGSDRTRLSKRHGATSVRFYKQEGYLPEAFFNYITLLGFGI
                     TEGKEIFTKEELISEFSIERCNKSNAVFDPEKLLWMNGYYIRNSTPERLFQLSLPFLK
                     KACLVKEPLSEEKKEEIKKLIYLHREKVKTLKEIPLWIDYFLTDEINYEEQCIKEYLG
                     ENSKKILSELLPYLEECSDFNKDLLESQLKKFFDEKKYKTKDVFHPLRVALSGRMRGP
                     GIFELLEFLGKEKVIKRLKRITNKETRK"
     gene            8408..8962
                     /locus_tag="PLW95_01005"
     CDS             8408..8962
                     /locus_tag="PLW95_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HOV21247.1"
                     /translation="MITRKKAEELKERAYKMLQKAGIVITEEEYKNMEVVDHGLNEPE
                     KYGVQIIVYVNNERYCAKEMVLIPGQTVCEHKHPPISRTNPGKQETFRCRYGEVYLYV
                     PGIPAKNPKAKLPEDREKYFTVWNEIILKPGQQYTIVPNTLHWFQAGKEGAIVSEFSS
                     QSLDETDIFTDPDVKRITEIAEND"
     gene            8955..10340
                     /gene="cysS"
                     /locus_tag="PLW95_01010"
     CDS             8955..10340
                     /gene="cysS"
                     /locus_tag="PLW95_01010"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014933939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOV21248.1"
                     /translation="MIKFFNTLTKKKEEFVPIEQNIVKMYTCGPTVYDFAHIGNFRAY
                     IFEDLLHRYLEYRNYKVIRVMNITDVDDKTIKGAKKEGISLKEYTDRYLNYFLEDMET
                     LRIKKPDFLPRATEHIEEMVSLIKKLLEKGYAYRKNGSIYYDISKFHNYGKLSGIELE
                     SVKPGARVETDEYSKEDVRDFALWKGVKEDEDVWWETELGKGRPGWHIECSAMSMKYL
                     GETFDIHTGGVDNIFPHHENEIAQSEGATGKKFVNYWLHCAHLLVNNEKMSKSKGNFW
                     TLRDLLNKNYEPVAIRYLLLTTHYRDPLNFTEKSLEGAENTIKNYNDFYNSLNFCQGK
                     EDEDIEKEIEKARNGFIENLDDDLNISPAIASIFNFIKYVNKKVAEYKVSNQQAEKIK
                     KFLEEIDYILCILNYKEKQLPEEYTELIKEREKARKEKNYKRADEIRDYLKSKGIIIE
                     DTPYGTRWKKE"
     gene            10337..10774
                     /locus_tag="PLW95_01015"
     CDS             10337..10774
                     /locus_tag="PLW95_01015"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HOV21249.1"
                     /translation="MKKGISLIEILVVLVIISIIASLSLPAIHKARLKGLITKTKTTI
                     DSLEAALSMYETDFGDYPESDGTGSKVLYQFLVGPIDDENWKGPYIRLRKQDIDEEGN
                     IVDAWNNPIIYKYPQEEYENTPYIIFSFGPDGKKNTKDDIGNW"
     gene            10806..11933
                     /locus_tag="PLW95_01020"
     CDS             10806..11933
                     /locus_tag="PLW95_01020"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOV21250.1"
                     /translation="MEYIEVVSDKKIHGWWKGKRECSSERVLLNPYVGCEIGCFFCYT
                     CGYPGKFQLSKKESKVFVYINFVDNLKKQIEKLNYISTCYLSPVSDPFQPAEKIFKIT
                     EKTIEFLISKNIPVNITTKETIPENIIEMIKGHPHCYGQVSILTIDENLRKKLMEKGA
                     ETDKLFENIRNLSKNNIFSICRIDPIIPFINDSQDTLEEIIKRSKDCGAKHIIASIID
                     INISKKEEIFTEIEKKFGRSIKRKLEELYIEKIGSSLHSKIDYRKNVFSLLRNLCDKY
                     NLTFALCMEFEKKDGKIYGLNKEFGSSVNCEGINIPIYKRDGEKFSPACDCNGNCISC
                     NFPSCGIEEIAQGKVKNNNYKGFDFYDYLRWSKKQKGLFEK"
     gene            11930..13855
                     /gene="rlmD"
                     /locus_tag="PLW95_01025"
     CDS             11930..13855
                     /gene="rlmD"
                     /locus_tag="PLW95_01025"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOV21251.1"
                     /translation="MKYILSSSSPRRKELLKRVIDDFEIVEPDVDEEKIKENDPIKYA
                     VTAATLKAKSVGEKRSGCIVLAADTIVSYKGKIIGKPQNYQEAMNILKELSGTEHKVI
                     TGIAIYNKDKNKLLTGYDISYVKFKNISEEDIKEYLEKNEFKDKAGGYAIQEIGDKFV
                     EKIKGSYENVVGLPIQKLKKMIEEFEMPEIEIEISDIAFPNNWGVGKKENFVVFVPGA
                     TYKDVVKVRIKKGEKSFSYGEVVEILSPSPYRKQAECPHFGICGGCSFQNLQYQIQLS
                     LKFNYIKKTLEKIGGIDIGKGEISEIVPSPCLYFYRNKMEFSFGGDKEIYLGLRERNS
                     PFEKYTWKVVSIEKCLIFSPLYEKISPIIIEFAKKTGLPPYNPYKKEGFFRHLVLREG
                     KNTGEAMAIIVTKSGKDIDFTGLVEKINDVAPEIKCLYWVENDQLSDVVSFEKKHHIW
                     GETKIEEKIGDFKFKIAPNSFFQPNSKGAELLYSEIYNNIGEGERVLGLYCGTGTIEV
                     FISNKAGEIIGIDSEEINILVAEENCQNNKIKNCKFYCGRVEKIIGNKNLGNFDTLIV
                     DPPRSGLTNKALMKLVKMEIPKLIYVSCNVSTFSRDINLLSKEGYKLEKLIPFDLFPH
                     TPHIEILGIIKKRSPLC"
     gene            complement(13883..14008)
                     /locus_tag="PLW95_01030"
     CDS             complement(13883..14008)
                     /locus_tag="PLW95_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21252.1"
                     /translation="MDKIIEALSKMPIEWDGRQIYYIGEKIRFFLKKVAVIFVSC"
     gene            complement(14001..15677)
                     /locus_tag="PLW95_01035"
     CDS             complement(14001..15677)
                     /locus_tag="PLW95_01035"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOV21253.1"
                     /translation="MAKVQINQRKIEFGANVDCPYLFPNLSLVITKQENLYKTKDKIY
                     SKFRYEKLNILINKFGLEKTWYSILATHIYNLHQPRFDYLLVQKEFSNAPILDIDILN
                     SISIGEIATLYEYSLSLLNNDSRKKHGQYFTPEDVAQVMAEKSLFFPKNRVWIDPCSG
                     VGNLSFWLVKLQETGEDFLTNQLYLIDKDALALFIARTLFTLAFQNKSKNLFHDIASH
                     FIVTDFLFSSNLPAFDFAFLNPPYVEVKSDNRFKTAEARNLYAYFLEKIITLSNGFIS
                     ITPQTFTNGQQFRTLRELLITKMRNISIYCFDNVPDTIFRGIKFGSTNTNKSNSIRAA
                     ITVAKSESTLNSFKITPLLRWRTTERTKMLESLDNYLTDVGVNADVFPKIQKELLPLY
                     YKVKKTKKCLVDIISTYPTKYKLIVPTTPRYFISALKTEVNRSSFRTLYFYNKKDYNL
                     AYILLNSSFFYWWWRINDGGMTISKKTLLSLPIPDNISADPILISKIENSELTNRVVK
                     KNAGKSSENVKHNIALIEEINQKLFPEFASVLNCLHNNSILNDNIRKFIG"
     gene            complement(15773..16720)
                     /locus_tag="PLW95_01040"
     CDS             complement(15773..16720)
                     /locus_tag="PLW95_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21254.1"
                     /translation="MESGIYEEIKYFKRVGCQTYQLLGWLGYKREDAFELENGKWEIY
                     TKRNFIFPYFVTNYSSPVNKFVAIYLNKENSIIVYGSFENIQKLKENLEIVTKTFFPV
                     KKKLFFIPLTLVEENAQDYGYIRGVLLGIALFFVDTLYSWIFKLRYGIFTGFIDFIKN
                     VYYGEPALAMGIGVLGTGLYFGFLLIFIPILLGNIYVKKEKKLREIKIKNLPDEIKEY
                     EFGVKAERALKEEFEMIIEEKKKEKMYCEINKLSSISKTDFETLYFELNQGFLSAEGL
                     LNFVNIYKNKLQNLPIEKFLEIISEYKKASIESEIKIIP"
     gene            16849..19218
                     /gene="gyrB"
                     /locus_tag="PLW95_01045"
     CDS             16849..19218
                     /gene="gyrB"
                     /locus_tag="PLW95_01045"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOV21255.1"
                     /translation="MDDIKNKYDATTIQVLSGIEAVRKRPAMYIGDTSIGGLHHLVYE
                     VVDNSIDEAMAGYCTKIKITIHSDNSVTVEDNGRGIPIDMHKTEKKPAVEVVMTVLHA
                     GGKFDRKVYSVSGGLHGVGVSVVNALSEYLEVEIKRDGNIYHQRYEKGIPVTQLKVIG
                     KTKTTGTKVTFKPDSKIFEVVEFDENILTGRFKELAFLNAGLEIIFEDERKALKERYK
                     FEGGIVSFVQFLNKNKKVLFEKPFYCKKVEEDVECEFSFQYNDGYSDTVLSFANNVNT
                     KEGGTHLTGFKSGLTKAINEYGKNNGFLDKISLSGEDVREGLTAVISVKLPEPQFEGQ
                     TKTKLGNSKIRFIVDSIAFNQIFTFLEENPEPARAILNKCITTAKAREAARKARDITR
                     KKLAETGALPGKLADCSSKDPEVRELFIVEGDSAGGSAKQGRDRNFQAILPLKGKIIN
                     TEKAAIDKVLNNEEIKMMISAIGGGVGDEFDVTRARYKKIIIMTDADVDGSHIRTLLL
                     TFFYRQMTDLIKHGYIYIAQPPLYKVKKGKKEIYVETEKEMDNILIDFATENAKMKIS
                     YDSKYIEIVEEKVREILSLSKNLNELIRTIENKGIKFKNLIDYYQKNKKIPQYYLVDN
                     VDKKEYYFDNYKQLSEFLGKKDEEIDLFSDEKSNYKIVEIYEKGQLEKILEKIKEFKI
                     APSEIFNKCFILINEEEERYSLIEIFEALKEKGKKNLFIQRYKGLGEMNPLQLWETTM
                     SPEIRTLKKVTIEDAIKAEEIFTTLMGDAVEPRKEFIEKFAKEVKNLDI"
     gene            19228..21666
                     /gene="gyrA"
                     /locus_tag="PLW95_01050"
     CDS             19228..21666
                     /gene="gyrA"
                     /locus_tag="PLW95_01050"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOV21256.1"
                     /translation="MEEKKIINIGVEEEMRQSYLDYAMSVIVGRALPDVRDGLKPVHR
                     RILYGMLELGLEPGKPFKKSARVVGEVMGKFHPHGDAAIYEAIARMTQPFTLRYPIIE
                     GQGNFGSIDGDPPAAMRYTEVRLAPIAMEILTDIDKDTVDFVPNFDESLVEPVVLPSK
                     VPHLLINGSSGIAVGMATNIPPHNIGEVLNALIFLLKKENATIEEIMEILPGPDFPTG
                     GIICGKEGIIKAYKTGKGIITIRGEIEIEEDDKGKKSIIIKTIPYEVNKASLVEQIAH
                     LAEIERIEGVSEIRDESDKEGMRIVIELKKGENEEIVINQLYKNTQLQTTYGIINLAL
                     SNQVPRLLNIKQMLSLFLDHRKEVIIRRSNFLLKKAESRYHILLGLIIALDNLDAVIQ
                     LIRKAKDVESARDGLIRSFKIDEKQANAILAMSLSRLTGMEREKIITESKETEKEIEK
                     LKGILADPEKVKNIIKEEFEEIKKKYADKRRTEIGGPVEEFEDIDLVRDEDIIVTVSN
                     DGYIKRSSLEYYKRQRRGGKGFIGAKTKEDDYVKHLFVTSTLDTILFFSEKGKVYWLR
                     GYLIPEGSRTSKGKPIVNLLKIDPGEKITATIPVKEFSSDKYLVMITKKGLVKKTPLI
                     AYSRPRKGGIIGINLREGDRLIDVKVTDGNQNILLATKYGFCIRFSEKEVRNVGRSSI
                     GVTGIRFKKENDEVVAGEIFSENELDLSLLTVTSKGYGKRTILSRYRKQKRGGKGIID
                     IKVNEKIGTIVGVKCVKLSDEVILTTKNGVVIRMDCKEIRAVGRNTMGVKLINLGSND
                     EIADIALVEKNEQV"
     gene            21653..22060
                     /locus_tag="PLW95_01055"
     CDS             21653..22060
                     /locus_tag="PLW95_01055"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HOV21257.1"
                     /translation="MSRYKIIDHTADIGIEVEGDSLEEIFFQSYKGLYEIAGIKKETT
                     GEKGKFEIKADTLEELLIKFLNELIYYIYVKNKFWEIKKLEIKMNKELKIKIEGNSGK
                     INKFEREIKAATYHNISIKKENGIFKTTIIFDL"
     gene            22416..22766
                     /locus_tag="PLW95_01060"
     CDS             22416..22766
                     /locus_tag="PLW95_01060"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOV21258.1"
                     /translation="MDKKEKEYYLKILLKEKNTILKNLGYLKEGIESVEKGVPTHIGD
                     LGTDEFVRGLGIDISHSEEQILRQIDEAIEKLEKGKYGICENCGKKIPKNRLKVIPYA
                     KYCVKCKKEMEKSE"
     gene            22759..23226
                     /gene="lspA"
                     /locus_tag="PLW95_01065"
     CDS             22759..23226
                     /gene="lspA"
                     /locus_tag="PLW95_01065"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOV21259.1"
                     /translation="MNKKSIIIFFLVFFISLILDQFSKTLVLEIIKDGEVKITKYFSL
                     IFVGNKGVLFGLFSNLPMNIPIIIISFFVILILCIYAINSSKESKILHLFLGLVAGGI
                     GGNLLDRIKYGYVIDFLDFKIWPVFNLSDVFIVSGTILTILYYIKRKKCIPNS"
     gene            23208..23969
                     /gene="lgt"
                     /locus_tag="PLW95_01070"
     CDS             23208..23969
                     /gene="lgt"
                     /locus_tag="PLW95_01070"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOV21260.1"
                     /translation="MHPEFIKIGNFVIYWYGVMVAIGIFVSCFIFQKISLKNGYSENI
                     TSKLLLWLIFWSIIGARVLHILVHFPYYYGHPLDIIKIRNGGLAIEGGIIAGLLFIVI
                     FGKINHLKTIEILDLVALVVPLGQAIGRIGCFLNGCCYGKKTDFIFGVKFPHLSYKVH
                     PTQLYYSFSYFIFFLFLWLLYKKKVKDGIIFSSYLLFFSLLRYLMDFLRGDLKGTFLF
                     LYPTQLIAIPIFFLGGILFMLFLQRKENMDGEIGV"
     gene            23950..24324
                     /locus_tag="PLW95_01075"
     CDS             23950..24324
                     /locus_tag="PLW95_01075"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOV21261.1"
                     /translation="MAKLVFNYLFMRKEYDLENFGKNEIYIGRMDSNDITIPDYVLFK
                     KLPLETQKLYIKDLVKISRIHSKLIKIGNNWFIEDIGTKGLGSNFGTYVNDARLEVQK
                     PYKLENNDKIRFGPFICTFVEE"
     gene            complement(24355..24642)
                     /locus_tag="PLW95_01080"
     CDS             complement(24355..24642)
                     /locus_tag="PLW95_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21262.1"
                     /translation="MPNYGGIWDNMKKDGEIKIDNLIDVWDKAEIINKLRMRRSYSEN
                     YGCKGKYKSVCGGCAARTYAYFGDILAPDPGCILNQKFNNISIEKEVISQN"
     gene            25071..25757
                     /locus_tag="PLW95_01085"
     CDS             25071..25757
                     /locus_tag="PLW95_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOV21263.1"
                     /translation="MKINEFYNGLDKRKKIILWIAIAFVFFSILRVSIWKAKSTNIPT
                     EKKICKQIYLPDKKKKIEGIYAIGQFKKEEYLPEVEKIFNSTDDVEIKRVAAWTIGKI
                     DIDKLKGYLDSQNKKEDKILVIDTLLKIDKNNINFLIERFPKEDKETKLYIMDIIEKE
                     EYSDKLMEIAENKDEEKEIRMKALSMLEKLGNNEHFSKLWNLYYNDTDTEIQMASYRT
                     IKEIEKRGTK"
     gene            25754..26428
                     /locus_tag="PLW95_01090"
     CDS             25754..26428
                     /locus_tag="PLW95_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOV21264.1"
                     /translation="MKNQKGGFTLIELLVVIAIISLLAGQLLPSLSSAREKGRQANCI
                     NNLHQIGLAIEMYYQDYNDYPTWLSILYKNYLGTPKIFLCLTDLYKGEKGCGNSLHPS
                     INDIPLSQIPNYTPDDFDSTAYSYRNPNITFCSYSYEFAPTKSEWFMAAHTSGEQQEA
                     DINSDGVVTWKEARIWQSNHGYGGQVPIVRCYWHFYNYGQKVINLAFRDYNVYSSGEE
                     WETSSY"
     gene            26462..28138
                     /locus_tag="PLW95_01095"
     CDS             26462..28138
                     /locus_tag="PLW95_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013681547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOV21265.1"
                     /translation="MEKKGVDFIREIILKDIEEGRYSKKVHTRFPPEPNGYLHIGHAK
                     ALHINYTIAKEFGGKFNLRMDDTDPEKETKEYVDAIIDNVKYLGYDWGEKVYYASDYF
                     DKFYEYAIQLIEKGKAYVCDLPPEKFKEYRGTLTQPGKESPYRDRTIEENKVLFEKMR
                     NGEFHEGKRILRAKIDMKNPNILMRDPVIYRIKKTPHYRQGNKWCIYPTYDFAHCLED
                     SIEGITHSLCTIEFEIHRPLYDWFLEQLSIHHPKQIEFAPLNLTYTILSKRKLTELVE
                     KKYVNGWDDPRMPTISGMRRRGYTPLSIKNFCNEIGISKTESLVNIELLHYFVRQHLN
                     KICPRVMVVLNPIKIVITNFPEGKVEYLDAINNPEDENSGIRKVPFTREIFIEKDDFM
                     EVPAQKFYRLSPGREVRLRYAYFIKCEKIIKNERGEIKEIHCTYDPDTKGGNAPDGRK
                     VKATLHWVSSSINTSVEVRLYDHLFTKENMNEIEEGKTFLDYFNNNSLKILRNCPAEI
                     NLKDVKEGEKYQFERVGYFCVDPDTEKGNNVFNLTVKLKDEWEKIKKGGN"
     gene            28140..28583
                     /locus_tag="PLW95_01100"
     CDS             28140..28583
                     /locus_tag="PLW95_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21266.1"
                     /translation="MEIKFTKEQYENLVKLVYLGNWMINAIRQGSNIIEKFEDLEQYI
                     FSFYNEFGLEGCIEYDEEDKRFYPTGKFEMESEIEEYRQEYDNENFWDELISRLARRD
                     FIRKYGEDAINKMTLEEIMEKESHFIEKYEEEFEKYGIERLEIKK"
     gene            28580..30574
                     /locus_tag="PLW95_01105"
     CDS             28580..30574
                     /locus_tag="PLW95_01105"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOV21267.1"
                     /translation="MSEDIYKQQEEQLKTKDIFDSVFKNTDTKYSLQEFSQKILGRIT
                     TFEKEPGRYYIRCLKRDKDIFIYDKNKNFGKPEEIIRQLWLIKLTQDYGYPLDRIEVE
                     KSVQFGREVKTKAADIVIYKEDKITPYIIFEIKEPNVKKAEEQLKSYLSAEGAEAGVW
                     SNGIQKFILYRPYPKEYITTFRDIPNVNQTWDDLFEEKRTYEKLQEKFDLTFIISRLE
                     EMVLAQAGVNVFEEVFKLIYAKLYDEDQAKNFRKEKEIHFKQYKEPKKTYAIINDELF
                     KGAIKEWPDIFNPIEKINLTPEQLQICVPFLEEIKLFGSGREELEIIDRAFEHLIADV
                     SKGQKGQYFTPRNVIKMCVKMLNPKENEYIIDPACGSGGFLLHTMYWVWDNYLRDTSL
                     EVKRRYANRYLFGIDFDDNMRRISQALMLIAGDGRHNIFKRNSLDSRDWIGEQAEEAR
                     ASLKPLLSKQNEENLKTYRYLNFDLLMTNPPFAGEITDSGLLRQYEFAKKNGKLKQKV
                     ERHLLFIERSLDALRPGGRMAIVLPQGIFNNTNMEWVREWFFEKARILAIVGLEGNTF
                     KLPSPAKGTGTKTSVLFLQKWEKEQRPLEDYHIFMAVSKKSGKDNSGEYIFVNEKGQR
                     VSSKNGFFKSQENLKLDDDLDEIAEEFIKFAKKEKFEWWI"
     gene            30576..31925
                     /locus_tag="PLW95_01110"
     CDS             30576..31925
                     /locus_tag="PLW95_01110"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOV21268.1"
                     /translation="MAVYSIIKKSQLEGTHRLDAEYYQPEYLEINRKISHFNNFFLGD
                     LIHSFASGKNLPQADNGKIFFLRTQNIGRVLINKEGMNFLAPNIEKYPVLNEGDILAV
                     RVGEGVGNTSIVTPKFAKHSYSDNVIRFRIEKLDPYFIIIFLNSRIGFNYLERVSKGS
                     ARSLISSENIYSIRVPDLGQKVSNFCKKAILEGAKEVEQSESLYLQAEDLLLEELDLK
                     NFQPEDDLSYIVNLSDVKKVHRADAEYFQPKYEKLVQKIKENKIEHLGNLVVMKKGIE
                     PGSNEYQNEGKLFIRVSNMSKYEITDKGQKYLSNELYQKLKDNFEPKIGEILLTKDAT
                     PGIAYVMKEPIEGIISSGILRLELKEKDIEEEYLALCLNSIIGQMQAERDGGGSIITH
                     WKPEQIKNLQIPILPKSTQQKITDLVRKSHQARKQAKQLLEQAKQKVEELIENSQNF"
     gene            32044..32205
                     /locus_tag="PLW95_01115"
     CDS             32044..32205
                     /locus_tag="PLW95_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018963509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HOV21269.1"
                     /translation="MTKKKYKDPVCRKYLAKETKNIVEKDGKVYYFCSPHCKEKFEKD
                     SEKYLKLVG"
     gene            32206..32766
                     /locus_tag="PLW95_01120"
     CDS             32206..32766
                     /locus_tag="PLW95_01120"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOV21270.1"
                     /translation="MEKVIPPLIALDVETTGLKPDEGDRIVEIALIKFDEKGKIIDKF
                     ISLINPEREIPLEVTKFINRIDKNMVEKAPKFKEVAKEIKNFIDGGIVVIHNADFDIS
                     FLKKEFQLCEEELPEFGVIDTLYIARKYFNFPSNSLSSLAKNLKIEKEPHRAESDAIT
                     AYEIFNHFVKKIDVEIDDLICSFGEI"
     gene            33027..33233
                     /locus_tag="PLW95_01125"
     CDS             33027..33233
                     /locus_tag="PLW95_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOV21271.1"
                     /translation="MAREKGKVKWFNNSKGYGFIERENGEDVFVHYSAIEGDGYRTLN
                     EGDSVEFDIVKTDKGFQAAHVKKA"
     gene            33314..34930
                     /locus_tag="PLW95_01130"
     CDS             33314..34930
                     /locus_tag="PLW95_01130"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOV21272.1"
                     /translation="MKKWIILFLSAGILYGEVDYLNMGLVAHQKGLYNLSNTQLEKYI
                     QQNPDGQYLDYAYLLYAKNLINLGKFEESKEKLTFLIENFPQSQYLKDAYTYTILVCL
                     ELSELNTSLEFYNVYIKKWGENKEIENQVCGKLIQRGIDSFQKGNYKKAIKCFNEVLT
                     ISNDTSFLDIANYYTALIYYQKNDFSKAKELFLKIKGENSKILPDVYLKIGDCYLNLK
                     DYNEAKRYYEKVVSTYPETETANWAMYQIAVIKKKEGKLNEGELILNRIKTNDNDLKL
                     QILQTLSEIYILEEKWTDAEKTILEIFQQFPSEKNPANYIKLAFINFNMKNMENSISY
                     FNSALNLNPSDELKEKIYFGLGYTYYLNGDIEKSFSFWEKIEKQFPESSFLPEIYYIK
                     GKKYFDMKEFTKSEKYLKNLVENYKESSFITQAIPLLIQIYIEEGNYKKAEEICQEVK
                     NKNEEINFLYGKTLYLTKEYEKAKYIFETTDIKNPQFKVESLYYLAQIYLYEGKEEKG
                     KEKLLEIVTYYPQFKEFYEKAEKQLKKYEK"
     gene            34927..36351
                     /locus_tag="PLW95_01135"
     CDS             34927..36351
                     /locus_tag="PLW95_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21273.1"
                     /translation="MKKFFLFIFLSSFLFAEEIKLPEEKIVGESKEIIKTKIFIFSPQ
                     TEEITFPEIEMPEIKIPEKEVKKEKKEEKKEEIINKNKENSIFISGGSFYTTDFNFSH
                     KGNNISFEIYNNYTEGFRENDEEYSSGINFYKKEEKYLVDINLKGGKKELTGPLFFPF
                     NIDRNWLNLQSGLTFYPGEKTSISIFQNYYKIDDLKTCFSFLEIKNDFDYFSSIFSFN
                     RQDFFHNFSQNSFSEGVYSDIGNTKIGGKIKVIENSGVRFVPYFKSPLIGGLNFEING
                     IYRIPDLWEDVINHTWAEIKQEELEPEEGYWVDLGYENENDRWQLKTGGKYTVWRNFY
                     TWSDVDNNFLFQPFPEKFNQFTLYLNHSFSLTDNIKYFLECEKAIRSKDINYFPENSV
                     NSGVILTYLPFILKLWTQYNGKRVSDGEQLDNFVVLNCDIKYKKGNYQFGVKVENITD
                     KKFFDVRGYPGERRKTLFYFEYYF"
     gene            36360..36995
                     /locus_tag="PLW95_01140"
     CDS             36360..36995
                     /locus_tag="PLW95_01140"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOV21274.1"
                     /translation="MSLWELLLKGGVIIIPIVFCGVLTIGIIIERLISLKNAEIDNEK
                     FISEIENTLRRRKIKEAIDLCEKNDNPVPRIIKAGLLKIDRSREEVKESIEDAANHEI
                     PNLEKYLGILATIATVSPLLGLLGTVTGMVKAFMVIEAKGGLVHPGELAGGIWEALLT
                     TVVGLVVAIPAYLAYNYFVSRVNNIVLDMEKSATRLIDIIFLIKEEDIANR"
     gene            complement(37156..39093)
                     /locus_tag="PLW95_01145"
     CDS             complement(37156..39093)
                     /locus_tag="PLW95_01145"
                     /inference="COORDINATES: protein motif:HMM:NF028438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HOV21275.1"
                     /translation="MSELRLENYIMPAAKLGPDSPLPPLDICLPYTLQNNYNREKKIT
                     EFKTVVLENDILKATFLIEFGGRLWSLFHKKAKRELLYTNPVFQPCNLAIRNAWFSGG
                     VEWNIGLHGHSVFTCSPLYVSKLKMDDGTPVLRMYEWERVRMVPFQIDAFLPDGSDFL
                     FVRVKIVNPNENEVPMYWWSNIAVPETPDTRVIVPAESILKRDYKGNKSIVSMSPDGA
                     IDGIDLSYPVNFNHSADSFYYIKPESQPWITSLDKEGKGLIQTSTKRLYGRKLFVWGM
                     HKGGRSWQEFLSVPGSAYIEIQAGLTQTQSEYIQMPAGAEWAWLEAYGLMECTPEVVH
                     GKNWKEAYNSVDKKIKSLMPENKLDLILKETTKMANKTPDEIIQRGSGWGTLERIRRE
                     KYNEPSFCSKALIFDDVSLKKEQEMYLELLISGLVPYVSPKEKIYGWMIQKQWQDILE
                     KAVKHPQNDHWLSWLHLGIMYYSQGNFEKAKNSWEKSLEREPSALVYRNLGILARKNN
                     HIEEAGDLLIKAVEMAPDVVDLIVECCNVLIKLEKYEGILKIINNLSSEIRNYGRIKI
                     IEAQVALKQNNLERVEEILKSRPSIPDIREGEQILTDIWFEMHERRIAKTENIPIDEN
                     LKKMVREKFSPPAWLDFRQFT"
     gene            complement(39116..40465)
                     /gene="radA"
                     /locus_tag="PLW95_01150"
     CDS             complement(39116..40465)
                     /gene="radA"
                     /locus_tag="PLW95_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOV21276.1"
                     /translation="MKEKTMYVCSECGYQSIRWLGRCPDCGNWNTFIQEVNIKTKIQQ
                     EGIIQPKKITEIKIEQQPRIKTKIEEFDRVLGGGVVPGSFILIAGNPGVGKSTLLLTI
                     SGIISNSNMKVLYVSGEESENQIKLRCERIKINFDNLYILSSNDINSVKNALEKMKPD
                     FLVIDSIQTLYNPDIPTTPGSVTQVKENANFLFNYTKRKNISTFIIGHITKEGIIAGP
                     KLLEHMVDTVLYFEGETKTNLRILRAIKNRFGPTDEIGVFSMEEDGLEEIPDASTIFL
                     SQDRKTLPGAVFFPSQEGKRTIIVEIQSLITPTYYGIPKRAVTGLDYNRISLVLAVLE
                     KKLNFNFSTYDVFMNVGGGFKITETGSDLAVAISAISSFKDVSPAENTIFIGEIALTG
                     EIRPVGQIISRIKECSRLRFERAIIPESNIKEVKEIKNMNLIGVKWLKECLDFSFSI"
     gene            complement(40708..41805)
                     /locus_tag="PLW95_01155"
     CDS             complement(40708..41805)
                     /locus_tag="PLW95_01155"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOV21277.1"
                     /translation="MNKRNLSLEFKVGLFAIITILLIFIFIFSQTKKGQWRTYEIGVM
                     FDYIGGLDISSPVRLSGVMVGEVKNIQILAKERPKVLVTLTLRRNVKVGKGSVITIRT
                     LGIIGEKYVEIIPSSEQQYLAAGEIIEGVNPISLEQFATIGQDIILNLNNVLVDLRKL
                     TSDIEIQKNVKEIIGDTSLAVKQMNSVLSKVDDLSTSVDKTNLEIRDIITKNSPKIEE
                     LINNTNFLILTSKDEIEKTSKEIRKFAKTSQDIEETVVKIGSTADEINTFFENLQNKG
                     LIAQIMNEENLLNQIKVEMEYLQDTTLKLKESAESFTDITENINSLFNYVNEGQGSIG
                     KFVKSDELYNEVLGLIKDIKAHPWKLFFRGR"
     gene            complement(41798..42541)
                     /locus_tag="PLW95_01160"
     CDS             complement(41798..42541)
                     /locus_tag="PLW95_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOV21278.1"
                     /translation="MEIIKVVNLKVVFEAKVVLDMLNLSVEKGESIVLVGSSGCGKTV
                     LIKTIIGLIEPVEGEIFINGQNILNLPEEKIREIRSRIGMVFQNSALFDSLTTWENVG
                     FYYLYHTDKSKEEIKKMAIEVLENVGLEGVEDLMPEQLSGGMKKRVSIARALISRPEI
                     LFYDEPTTGLDPITSSNITSLMKKIHLKFNTTDVIVTHDIKLAREISDRIGLVENGKI
                     VEIGTFDFLREKSNHPLIRSFVEMEGKNE"
     gene            complement(42541..43314)
                     /locus_tag="PLW95_01165"
     CDS             complement(42541..43314)
                     /locus_tag="PLW95_01165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOV21279.1"
                     /translation="MKNFIENFGKKIVAPVYYFGSLILLIGNSLKKAFTERKRENILK
                     HIEEIGINSLPLVGVIAIFTGMILVMEIGHTLKAFGAETYSGALVGLSMARELGPVLV
                     ALTLAGRVGASIAAEIGVMKITEQIDALKVLAIDPVSYLISPRVIAGIFSLPALFVIS
                     FFLAIGGAFLIGVFLIKIPSGQFLYQSFRFISYKDFLVGFIKVIVFAIVVINVSSYEG
                     LKAKGGAEGVGDASTSAVVKSFFLIILTNLILTGIFYFV"
     gene            complement(43315..44667)
                     /gene="dnaB"
                     /locus_tag="PLW95_01170"
     CDS             complement(43315..44667)
                     /gene="dnaB"
                     /locus_tag="PLW95_01170"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOV21280.1"
                     /translation="MSAKKKDLNIEKIPPQNIDAEKALLGCMLIDEEARIRVFETLKK
                     DFFYSSSHQQIFSSIIKLYERNEKCDIITLTNQLKQDGTLDEIGGVEYITEIAEFVPT
                     SAHIEEYIKIVKDQYILRSLISNATQIITEASNGVEDVERLLDKAESLIFEISQNKID
                     RYAYPLKDLIKENLDIIENIQNKKGFVTGISTGYFDLDKYIGGLHPSELIIIASRPSM
                     GKTALATNIALNLAASGDNFPILFFSLEMSKEQLVGRMLCCEARIDYKKLRDGILSDK
                     EITKLLLSAGRLEKAPIFIDDAPSLNVFELRARARRLKAKENIQLIIIDYLQLMKGVR
                     RTENRQQEITEISASLKSLARELNIPVIAISQLSRATEQREDKKPHLSDLRESGSIEQ
                     DADVVLLLYRDDYYKKEDSEKPGIVDLIIAKQRNGPTETVNLTFIKEYTRFENYTKRE
                     "
     gene            complement(44669..45115)
                     /gene="rplI"
                     /locus_tag="PLW95_01175"
     CDS             complement(44669..45115)
                     /gene="rplI"
                     /locus_tag="PLW95_01175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOV21281.1"
                     /translation="MKVIFIKELSGKGRTREIKNVKDGYARNFLIPSGYAIPATDQNI
                     KMIKEKEIIENRKKENRKKMALDTKKALSNVSLTITAKAGQDDKLFGAITSEIISEEL
                     KNQVKIDIDKHQIVLDEPIKKLGRYKIPVKLGDEVEGEIKVWIVRG"
     gene            complement(45127..45342)
                     /gene="rpsR"
                     /locus_tag="PLW95_01180"
     CDS             complement(45127..45342)
                     /gene="rpsR"
                     /locus_tag="PLW95_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002599159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOV21282.1"
                     /translation="MVKTRNRQCAFCKGKVKEIDYKDIGKLRRFISGRGKILSSKLTG
                     TCAKHQRMLTKAIKRARFIGLLPYVKI"
     gene            complement(45323..45715)
                     /gene="ssb"
                     /locus_tag="PLW95_01185"
     CDS             complement(45323..45715)
                     /gene="ssb"
                     /locus_tag="PLW95_01185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOV21283.1"
                     /translation="MPNPNLNKVFLIGRLTRDPELRYTSSGQAVTTCRLAVNREYSAK
                     TGEKKEETCYVNLVVWGKRAEVAAEFLKKGNLIFAEGRLNYRTWENNDSEKRSVLEVV
                     VENFQFLEKPNVDIIEEKEDEINGKNQE"
     gene            complement(45696..46022)
                     /gene="rpsF"
                     /locus_tag="PLW95_01190"
     CDS             complement(45696..46022)
                     /gene="rpsF"
                     /locus_tag="PLW95_01190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOV21284.1"
                     /translation="MEGKYEVCFVLRSDLPDDEVEKEIEYIEKVLVNGGAKIFKKENL
                     GRKILAYPIKKRTEGIYFVFYFTAPGTIKETIDSLKLRENILRYLLIRRKILPHEEII
                     NAESQS"
     gene            complement(46157..49129)
                     /locus_tag="PLW95_01195"
     CDS             complement(46157..49129)
                     /locus_tag="PLW95_01195"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOV21285.1"
                     /translation="MKNENISYDGKSFIFNGKRVFINSGEIHYFRIPESQWKDRLLKC
                     KRAFLNTIGTYIAWNWHEGKEGEFSFEKDRDINRWLSLAEEVGLYIFVRPGPYICSEW
                     DFGGLPNYLIPKDCELRSLEQNYIGYCKKYLNTVNKIIKPHLITNGGKIFLYQIENEF
                     EVGDIPYHRKLKEIVEKDGINIPICTNENFWIRGTDIIEGPDPYHFSWVISEVMNKIR
                     NLLKTQPDKPPLGIEVGTHFYSRFYGQMPFSDGYKPPELDEVYLKCLIAAGISGFNRY
                     MFHGGTNIGYWTGRDISTSYDYEAPIREWGELDKRYYIVRRIGGFLECFQEALLETIP
                     VEGECSVNEKGVEIFMRKNNKSVFMFLSNTYGKNKEFKINFKDPETGKNIIFPAKNKY
                     QLPGYSATIIPVNLKLSDDLTLLYTTSEIFYFIDNGKEKILILYREDGIDGEISLKIG
                     ENIKNVNYTHKNNPQFIVVERDIKLIIIDKEISSKTWFFNYKNKKYPIFSNIYFLEEE
                     KERENNIHLVFQVKEGKNWMKIPFKPLEVKLDNENIEYSYYEENNITEINLPEDKFPE
                     IHYELSGKWKVKEENIENNSDKWLKWRPLECLENYGFLKNGYSIYKSNFNLSKVKKPL
                     YLTLTGFQDEASIYINGKYVNSGRDNLRCEVSSYLSKGDNSLIVLVESEGHVCSGEKS
                     FNGIHSPVYLSSEEKSIELKWWRRKFIPDTYPERAIIREEMEEINDEFDDSSWEKVRV
                     DKKFDSRLFKNSYEECFAWYRTEVEIPDDFEKRGISIDFESGMNAKIYLFVNGKFSGI
                     KENAFLSLPFSFDLTNKVKKGKNYITIGIKVDRWGTHYGLNGTVKITSYDVCLNKNWQ
                     VREGIEGQTRGYFSPEFDDSDWDDVEINKEKWASGSLIWFRKKVKIENIPDYISPLRI
                     TLKNTSEKALIYFNGVLIGRYSEKGGQEDFYIYEDLIKEENVISVMVDGREKEAKLGE
                     ISITPYYISKRISVDIKL"
     gene            complement(49635..50603)
                     /locus_tag="PLW95_01200"
     CDS             complement(49635..50603)
                     /locus_tag="PLW95_01200"
                     /inference="COORDINATES: protein motif:HMM:NF012300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOV21286.1"
                     /translation="MVVNLPPQYHEKEKELKEVKTPEEKIAVLEEMLSIMPKHKSSEK
                     LQALIKSKISKYKKMMEKSPSTAKKSIVPQIPKEGDAQVVICGPTNSGKSTLLTTLTK
                     AKPEIADYPFTTTVPIPGMLQYQDIKIQMIELPSLSLDFTDNWVGDFIRKSNLILFLF
                     DLSSDDLIEEIEDAIGVLEKFKVKKEGEFQFFYKILWVGNKIDVSSHKEIKDVFVELY
                     KDKIPYFFEISAKEKINIEKLPEIMFNHLEIIRVYTKIPGKSPDIENPYTIKKGSQLI
                     HLAEIIHKDMVKSFKYARLWRGKTETPIVIGRDYILEDKDIVEIHF"
     gene            complement(50606..50983)
                     /locus_tag="PLW95_01205"
     CDS             complement(50606..50983)
                     /locus_tag="PLW95_01205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV21287.1"
                     /translation="MGEIKKVLVIEDDSVERQVLLQALKNSGYVSYGAQTGEEGLTLF
                     KKVKPDVVLLDVVLPGIDGWDVLKKIKSGPLSRKVPVIMITAKSENTDRITGYEIGAD
                     FYVTKPYNISKLLPIIRNIVSEK"
     gene            51143..51787
                     /locus_tag="PLW95_01210"
     CDS             51143..51787
                     /locus_tag="PLW95_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21288.1"
                     /translation="MKKELEMGIRKKISKKIAERYQRGQKKEKGKILDEFVSLMGYNR
                     NYASWLLRNIGRKVVIKGKRGRVILIGEQQKIKREGRKKVYGKEVEKALKEVWYILDC
                     PCGRRLGDYIGEIVPILEEKGEIKITEEVRDKLLKISGRTIDRLLKEEKKKWEIRGKK
                     RTKPGSLLKSQIPIRTFAEWDEKVPGFIEIDLVSHDGGKEEGIVVKSSSPFNNF"
BASE COUNT        18928 a         7307 c         8039 g        17632 t
ORIGIN      
        1 ccccctattt ccctccttcc ttcctttctt ccctctcccc tgcggggaga ggctcgcccg
       61 tagggctacg cctgaggggt ataggtgagg gacgatttca tctttttttt gacactacct
      121 ataaaatgaa cttccatatt taataaatgt ttttaccata acttttattt tttcccaact
      181 atggccaatt gcttgtggag atggatacca ttttgcaata aatccaccat taaattttcc
      241 aaaataataa attagttttt ttgcataatt tctaacatca tccaatgttc catttatcat
      301 aacactttga atatcaacag gacatcagaa acatatatgc cctccaaacc tattacttaa
      361 attttccaca tccatatttt cctgttggtc catctgtata acattcaatt tactttcaat
      421 taaaccatca agtatgtcaa caatatagcc acaactatga agatttgggg attgaaataa
      481 tattgctttt ttactatctc ttttgaaatt atttttattt ccactgatag acaataactg
      541 gctgtttttt taatatattc tcatctatgt tataaaattt actgataagt aatttttgta
      601 ttatgtctgc ctcaatttct cttgcgataa aataagagaa aattacttca attccatagg
      661 gaataatttt gccttctttt atatagttca ttattgcttt gtatcttata atttcaaaat
      721 tttcttcatt tataaaagta tctgtaattt tatattcttt tgtaagggat tttaaaattg
      781 cctcttcatt taaaaatttt tctgcccttg tatttccgtt tctaataaaa aaggatgata
      841 ctattttttc tccttttttc ttttttaaaa taaaagtaga aaggttagca aagtcaattt
      901 caattttccc atatttttca ataaaggaag aattaaatgc agttaataaa ttatatagtt
      961 gaatataata ctcatttttc aggtcaagta atttttcttc tatatttttt tctgatttta
     1021 ctattttaac ccctttcttt aaaaaatcag gtatattccc tttatttcct tcaatatatc
     1081 ctttcaaaat ctgaccgggt aaattacatg gtaaaacata attactttta ttttctaaaa
     1141 taatcctcaa attaaaaaaa tcatttttta ggagaaaaat tctcaaaata tcaggagaaa
     1201 ttttttctat ttcttcataa ataaaatttc tctctttatc aagtaattca ataatttctt
     1261 ctgtatttaa aattggtgaa ggaaatttat aaaatgactt ttctaatatt gaaataacct
     1321 ctcctatatt ttttgcctct attatacttt gtacctgttt ggatgttaga aatttttttt
     1381 caagtgcctt aattcttcca ctttcagaat aaatatccat tttcttcacc ttctttatta
     1441 tagtactttt ttctttctat caactctctt attttttcaa agtccttttt aagaagtaaa
     1501 tatctgtctg gattataaac agcaacagca ggatgataaa gtggcaatat aaagacacct
     1561 tttttttcat aaatatttcc atgcacctta cttattgctc ctgtctcatc tattgaaaat
     1621 ctgttaaaaa tttctctcat tgaatgtctt ccaagaggga caataatttc tggttttatt
     1681 agttcaattt gttttgataa gaaataagaa caacttttta tttcttcatc ctctgggtct
     1741 ctattgttag ggggcctaca tttaactata tttgttatat aaacatcatc tctttttaag
     1801 ttaattgacg aaagaagtaa gtcaagaatt tctcctgctt ttccacaaaa aggaatgcct
     1861 tttttatctt cattaaaacc aggaccttcc cctataaata aaattctggt atcaatactt
     1921 ccttttccaa aaacaacatt tgtcctacct ttatataatt tacatctttg acaatttcta
     1981 ctttcttctt ctaattcctc aaattccttt ttcttttcca ttaaataatt tttctgcaac
     2041 ttcttttctt agttcctgac caattgtctt tttaatattt tcccaggaaa actcaatatc
     2101 actattctct ccttcaatta aaaatttatc ttctaaatgt ttttctttta taacttcata
     2161 tccatcaatt tttaaattta cattttcagg aactaaaatt tttacttttt tatcatttat
     2221 tgtttgtaat atttgaaata taatttctcc aatatttttg ttaagatatt cattaaattt
     2281 attttttatt ttttcttcac atttatctat caattgattt ttaaaggaaa gaatttcttt
     2341 atttttttca attttaaagt taataatttc attttctatc tctttatcaa ttgtttcttt
     2401 catttttttt aatttttctt catattcttt ttcaataaga aattttttct tttcccattc
     2461 agaattcaaa acactttgtg cctcgtcaat tatttttttc cttttttctt ctgcttcttc
     2521 catcaatttt ttaataaggt tttccatttt atatttttac tacaaaaagt aaaaaaattg
     2581 aagcaacaag tcctaaaaca gcatatgttt caaccattgc tgcataaacc attgccctca
     2641 tacttgcttc tggatgtttt gaagcaacag atattccagc agcacatacc cttccctgat
     2701 atattgcaga agtaagtcct gaaattgcta ttggcaaaca tgaaagaaga atttgtagac
     2761 cagcagatgg agaaactgtt actatttgcc ctccaaggat tccgactttc atcattataa
     2821 gaaatccagt caaaaaccca taaattccct gtgtccctgg aagagcaaca agcaataaca
     2881 ttgttccaaa tttttccggc tcttctgaaa gaacaccatt tgctgcacta cctgaataac
     2941 caattccaat acttgaacca gtaccacaca aaaatatagc aagtcctgct ccaattaaag
     3001 catacaaaag tccgaggttc atttttatcc tccttttatt tgttttaatt ttttaataag
     3061 taaaggtaaa atttcaaaaa catctccaac aattcctaaa tttgcaactt taaatatagg
     3121 tgcatcaggg tctttattta ttgcaataat atagtcagat gtttgcatac ctgcaagatg
     3181 ttgaattgct cctgaaattc cacatgcaat ataaattttg ggttttacag tttttccagt
     3241 ttgcccaacc tggtgaggat agggtatcca accagaatca acagcagcac gagaagaacc
     3301 aactgctgcc cctaaaagtc ctgcaagttc ccttataagg gaaaaatttt ctgctgcttt
     3361 tattcctctt ccaccagaaa taattatttc tgcttcctgt aagtcaacaa cattttcaac
     3421 tctttctcta ccaataactt caacattttc ttctatatca atctcaatat tttctttaat
     3481 tattttccct tctttcccat caattacttc tgcaggagag aaaatttttg gtcttattgt
     3541 tgccatttga ggacgatgat ttttacatat aattcttgcc attaaatttc caccaaaagt
     3601 tggtcttgtc tgaactaata gccctgtttc ttcatcaata tcaatatctg tacagtcagc
     3661 agtaagtcct gtttttattc ttgcagcagt aaaaggtata aatgaccttc caatcattgt
     3721 agcagcagca atgacaatac ttggcttgta ttttttaata agtttagaaa atgcatcagc
     3781 aaaagtttgt tctttgaatg tctgtagttt ttcattttcc acaatataaa cattatcaca
     3841 tcccctttct attagttgag aagcaaaaga ggacacatct ccgcctaaaa gaactcctga
     3901 tatttcttca tttaattttt cttttagttt caaacaggca gaaagcattt cataagaaac
     3961 agaagaaatt cctttttctc ttgtttcagc aaaaaaccat attcctttat aatcagatgt
     4021 atcaattttt atttcttttt cttctttata aagtgcctgt tcaggacagt tctttataca
     4081 taatccacaa agggtacatt tttcatttat attgatttta ttttctttta tttcaagtgc
     4141 accaaatggg caactttcaa tacaattttt acataaatta catttttcat aaattatttt
     4201 tatcattata ctactcccaa tgttttaagt tgttttatta tttcatcagt taaatattct
     4261 ggctcaccct caattttttt accttctttt tttatttcag gtgtccatac atcaataact
     4321 cttgttggag aaccatcctg tccaaaaaat ctctcatctt cgcctaactg gtcagcagtt
     4381 attcttttaa tttctgcctt ttttgctttt aatagtcctt ttaatgaggg aattcttggt
     4441 tcatttattt cctttacaac tgttattaaa acagggagtt ttgccattac cttttcatag
     4501 tggtcttcca ttaatcttgt gcaataaata atattatcag aaatattctc aattttactt
     4561 acaaaagtta tataagggat tttaagaaat tcagcaattt caggtccaac ctgtccggta
     4621 cttccatcaa gggtttctct tccgcatatt attatatcaa aattaccagt tttttttatt
     4681 ccacaactta aagtaaaaga tgtcgctaat gtatcacttc caccaaattt tttatcagta
     4741 agcaaaaaac catcatcaac tcccatcgca attgcttctt ttaatgcttc ttctgcctga
     4801 aaagggccca tagaaattgc caatacttct cccccaaacc tctctttcaa tcgtattcct
     4861 tcttcaatag cataggtgtc ataagggttt ataatacttt ttactccttc tctaacaatt
     4921 ctttttgttt cgggatctat ttttacatca agtgactctg gaacctgttt tatgcatacg
     4981 actattctca tcctctctcc ttttcctgtt taatgtgaag aataaaatat ggtataatta
     5041 taccactgat tttgcttttg taaatttttt tgtgggaatt ctaccgactt taataataaa
     5101 attttaggag attaaatgga gaaaaagaga aaatcgtttt acaataagtt tgaaattaaa
     5161 aaaaaggggt caattgatga aaatatcaat aaacttcaaa acgaattaga tatttatcta
     5221 aaaggagcag aagagatagt agaggaagat gaaattttag aaaaaaaaga tattaaaaaa
     5281 cctgaaaaaa aggcatcttt taaaaaagaa gaatctaatg aaaaatataa agaaagtact
     5341 tataaacata aagatattac tgcaaatgta aatgctaaat ttaataaaaa aatattgttt
     5401 attccacttt ttctttttat actttttatt ggatttataa aatttcctct ttttaaatat
     5461 acaacaactc caaagaaaga cagggacata ccaggtattg aaataaagac aattttttat
     5521 tacaatgttt taggaacaaa attaaaagaa aagacaattc ttaatgtagg aaataaagaa
     5581 ataatagtcc cgatttctat tgaaaaatgg tttggcctat caaaagagga gaaaataaaa
     5641 tatattaagg tttttcagag tgaatgaatt ttaatcctct tttatatttc ccttctaatt
     5701 tctgctaact tttttatacc cagtttatta taaaaatagc cccaacggga tttgaacccg
     5761 tgtctgatgg ctgagaacca cctgtccttg gcctctagac gatggggcct gtacttatca
     5821 atttgtgtta aaataatact taaaggataa aataatgtca agatattata aaattgcagg
     5881 atgtattcat atacattttc ctattaaatt ttttgagaaa ggaataaagt tagtcgcaga
     5941 gaatgcaaac cgtgcaggtc ttgattttgc cattctaaca agtcatgtgc cagaaaaaga
     6001 aaataagtat aaagaaattc taaattttaa ttcttattat gggaaaacac ttattatttc
     6061 tggacaggag acaaatgata taagaaaaaa gaatcatcta ataattgtcg gggaaaaaga
     6121 gtggtatagg aagaaagaga atatccagga gataatatat aaggtaaaaa ataaaaatgc
     6181 ttttacaata gttgcccatc cttttggtga gcatagatta tctttaaaaa agaaaaatta
     6241 tgaatgggaa aattgggaaa acgaatttga ttgtgttgag gtatggtcat tgctttttga
     6301 ttgggcaact tttacaaatc catttaattt acctttcagg tattttacct ttcctgataa
     6361 cttaaatggt cctgataaga gggcagtttt aatgtgggat agcatttcaa acaaaagaaa
     6421 aattcctgct attgctggtc ttgatatcca tttacttcca ccaattttaa gattttttga
     6481 tataaaaaag aattttcttt atgaaacaac atttaaaatt ttaagaaatc atcttttttt
     6541 aaaagaaaac ttgtcaggaa attcattgga tgatgttaag aggatatttg agtgtttaaa
     6601 aagaggaaat ttatattttt ccaatgatta cttaaaaaat agtgatggat tttattttgg
     6661 tgaagaaaat ggggactttt taatggggga tgaagggcaa tttgggaaga aaataattat
     6721 agaaacgcct tttaaaggtg aaataaaact tataaaaaat gggaaagtaa tagttagaaa
     6781 aaatgataaa tttcttgaat attttgttga ggaaaaggga aattataggg ttgaagttta
     6841 tttcaataat aaaccatgga ttttttcaaa ttttctctat tttggtagtt tttgttaaat
     6901 aaaatctact aaaacgcata cacatacttt acttatctta tttattctgt taaaatcact
     6961 atgaaaggag aaaaatggta agagtaagat ttgccccaag tccaacaggt tatctccata
     7021 ttggaaatgc aagaactgcc ctttttaatt atctttttgc aagaaaaaat aaagggaaat
     7081 ttgtattgag aattgaagat actgataaag agaggtcaaa aagagaatat gaagaaggaa
     7141 taattgaaga ccttaaatgg gttggaattg attgggatga aggtcctgat attggtggtc
     7201 catttggacc atacaggcaa tcagaaagat tatctattta cagagatatt gcagaaaaac
     7261 tattgaaaga gggaaaagca tataagtgtt tttgcacaaa agaagaagtt gaaggaagaa
     7321 caaaagaaac tggatatgat ggaaaatgca gaaatttaac aaaagaagaa attgaaaaat
     7381 tagagaaaga aagaaaagaa tatgttttaa gatttaaagt acctgaaaag aaaaaggtta
     7441 aggtaaatga ccttataaga ggagaagttg aatttgaaac ggattttata actgatttta
     7501 ttataatgaa accagataat actcctactt ttcattttgc agttgtcgtt gatgatgttc
     7561 tgatggaaat aactcatgta attagaggag aagaccatct ttcaaatact ccaaaacata
     7621 taatgttatt tgaagcagta ggcggaaaaa ttcctcaatt tgctcatatg tcaatgacac
     7681 ttggaagtga tagaacccgt ttaagtaaaa gacatggtgc aacttctgta aggttttata
     7741 aacaggaggg ctatttacca gaggcatttt ttaactatat cactttactc ggatttggaa
     7801 taacagaggg aaaagaaatt tttacaaaag aagaattaat atcagaattt tctattgaaa
     7861 gatgtaataa atctaatgct gtatttgacc ctgaaaaatt attatggatg aatggttatt
     7921 atatcaggaa ttcaacacca gaaagattat tccaactttc tttaccattt ttaaaaaaag
     7981 catgccttgt aaaagaacca ttatcagagg aaaagaaaga agaaattaaa aaacttatat
     8041 atctgcatag agaaaaggta aaaacattaa aagaaattcc tttatggatt gattattttt
     8101 tgactgatga gataaattac gaagaacaat gtataaaaga ataccttggc gaaaatagta
     8161 aaaaaattct ttctgaactt ttaccttatt tagaagaatg ttctgacttt aacaaggact
     8221 tattagaaag tcaattaaaa aaattctttg atgaaaaaaa atataaaaca aaagatgttt
     8281 ttcatccatt aagagttgca ttaagtggca gaatgagagg tcctggaatt tttgaacttc
     8341 ttgaatttct tgggaaagaa aaagtaataa agaggttaaa aagaataact aataaggaga
     8401 caagaaaatg attacaagaa aaaaggcaga agaattaaaa gagagagcat ataaaatgct
     8461 tcaaaaagca ggaattgtta taacggaaga agaatataaa aatatggaag ttgttgacca
     8521 tggattaaat gaaccagaga aatacggagt gcaaattatt gtttatgtaa acaatgagag
     8581 gtattgtgca aaagaaatgg ttttaattcc tggacagaca gtttgtgaac ataaacatcc
     8641 tccaatatcc agaacaaatc ccggtaaaca ggaaacattt agatgtagat atggagaagt
     8701 ttatctttat gtacctggta ttcctgctaa aaatccaaaa gcaaaattac cagaagatag
     8761 agaaaaatat tttacagtat ggaatgagat tattttgaaa ccaggtcaac aatatacaat
     8821 tgtcccgaat acattacatt ggtttcaggc aggaaaagaa ggagcaattg tttctgaatt
     8881 ttcttcccaa agtttagatg agacagatat ttttacagac ccggatgtta aaagaataac
     8941 agagatagcg gaaaatgatt aaatttttta ataccctgac aaagaaaaaa gaggaatttg
     9001 tacctattga acaaaatata gtaaaaatgt acacctgtgg tccaactgtt tatgattttg
     9061 ctcatattgg taattttcgt gcttatattt ttgaggactt actccataga tatttagaat
     9121 acagaaatta taaggtaata agagtaatga atataacaga tgtagatgat aagacaataa
     9181 aaggagcaaa aaaagaagga atatctttga aagaatatac tgataggtat ttgaattatt
     9241 ttttagaaga tatggagaca ttgagaatta aaaagccaga tttcttaccg agagcaactg
     9301 aacatattga agagatggta tctcttataa aaaagttatt ggaaaaagga tatgcatata
     9361 ggaaaaatgg gtctatttat tatgatattt caaaatttca taactatgga aaactttcag
     9421 ggattgaatt agaaagtgta aaaccagggg caagagtaga aacagatgaa tatagcaaag
     9481 aagatgtaag ggactttgca ttatggaaag gtgtaaaaga ggatgaagat gtatggtggg
     9541 aaacagaact agggaaaggt agacctggat ggcatattga gtgttcagca atgagtatga
     9601 aatatttagg agagacattt gatattcata cagggggtgt tgataatatt ttccctcacc
     9661 atgaaaatga aattgcacag agcgaaggag caacaggtaa aaaatttgtg aattactggt
     9721 tacattgcgc tcatttactt gtaaataatg aaaaaatgtc aaaatcaaaa gggaattttt
     9781 ggacattaag ggacttgttg aataaaaatt atgagccagt tgccattaga tacttgcttt
     9841 taacaactca ttatagagac cctttaaact ttactgaaaa atcattagaa ggtgcagaaa
     9901 acacaattaa aaattataat gatttttata attccttaaa tttctgtcag ggtaaagaag
     9961 acgaagatat tgaaaaggaa atagaaaaag caagaaacgg atttattgaa aatttagatg
    10021 atgacctgaa tatttctcct gcaattgcat caatttttaa ttttataaaa tatgtgaata
    10081 aaaaagtagc agaatataag gtatctaatc aacaagcaga aaaaattaaa aaatttcttg
    10141 aagaaattga ttatattcta tgtattctta attataaaga aaagcaactt ccagaagaat
    10201 atactgaatt gataaaagaa agagaaaagg caagaaaaga gaaaaattat aaaagggctg
    10261 atgaaataag agattatcta aaaagtaaag gaataataat tgaagatacc ccttatggaa
    10321 caaggtggaa aaaagaatga aaaaaggaat atctttgatt gaaattcttg ttgttcttgt
    10381 aattatctca attattgcat ctctttcgtt gccagcaata cataaagcga gattaaaagg
    10441 actgattaca aaaacaaaga caacgattga ttctcttgaa gcagcacttt caatgtatga
    10501 aaccgatttt ggagattatc ctgaatcaga tgggactgga agcaaagttc tttaccaatt
    10561 tcttgttgga cctattgatg atgaaaattg gaaaggacca tacataagat taagaaaaca
    10621 ggatatagat gaagaaggca atattgttga tgcatggaat aatccaatta tctataaata
    10681 tccacaggaa gaatatgaaa atactccata tataattttt tcatttggac ctgatggtaa
    10741 aaaaaataca aaagatgata ttggcaactg gtaattgtaa taaggacgca tcctgaatat
    10801 caataatgga atatatagaa gttgtctctg ataaaaaaat acatggctgg tggaaaggta
    10861 aaagagaatg ttcctctgaa agagtccttt taaaccctta tgttggatgt gagattggtt
    10921 gttttttctg ttatacctgt ggataccctg gaaagtttca gttatcaaaa aaagaaagca
    10981 aggtatttgt ttatataaat tttgttgata atttaaagaa gcagatagaa aaattaaatt
    11041 atatttctac ctgttattta tctcctgttt cagacccatt tcaaccagca gaaaaaatat
    11101 ttaaaataac tgaaaaaaca attgaatttc taatttcaaa aaatatacct gtcaatataa
    11161 caacaaaaga aacaatacct gaaaatataa ttgaaatgat aaaaggacat cctcattgct
    11221 atgggcaggt atcaatttta acaatagatg agaatttaag aaagaaattg atggaaaaag
    11281 gggcagaaac agacaaactt tttgagaata taaggaattt atcaaaaaat aatatttttt
    11341 cgatttgtag aattgaccct attatccctt ttataaatga tagccaggat acccttgaag
    11401 aaataattaa aagaagtaaa gattgtggag caaaacatat aattgccagt attatagata
    11461 taaacatttc taaaaaagaa gaaattttta cagaaattga gaagaaattt ggcaggtcaa
    11521 taaaaagaaa attagaggaa ttatatattg aaaaaattgg ttcgtcctta cactcaaaaa
    11581 ttgattatag aaaaaatgtc ttttcattat taagaaattt atgtgataaa tacaatctta
    11641 cttttgcttt atgtatggaa tttgagaaaa aagatggtaa aatttatgga cttaataagg
    11701 aatttggtag ttcagttaat tgtgaaggca taaatatccc tatttacaaa agagatggag
    11761 agaaattttc ccctgcctgt gattgtaatg ggaattgtat tagttgtaat tttccttcat
    11821 gtggtattga agaaattgcc cagggaaagg taaaaaataa caattataaa ggttttgatt
    11881 tctatgacta tttgagatgg agtaaaaaac aaaaaggact ttttgaaaaa tgaaatatat
    11941 actatcttca agttcgccaa gaaggaaaga actattaaaa agagtgattg atgattttga
    12001 aattgttgaa ccagatgttg atgaagaaaa aataaaggaa aacgacccaa taaaatatgc
    12061 agtaacagca gcaaccttaa aggcaaaaag cgttggagaa aaaagaagtg gttgtattgt
    12121 ccttgctgct gatacaattg tctcatataa agggaaaatt attggcaaac cccaaaatta
    12181 tcaagaagcg atgaatatac tgaaagaact ttcagggaca gaacataaag ttataacagg
    12241 aattgcaatt tataataaag ataaaaataa acttttaacg ggttatgata taagttatgt
    12301 taagtttaaa aatatttctg aagaagatat aaaagaatat ttagaaaaaa atgagttcaa
    12361 agataaagca ggaggatatg caattcagga aataggagat aagtttgtag agaaaataaa
    12421 aggaagttat gaaaatgttg tgggactgcc tattcaaaaa ctaaaaaaaa tgattgagga
    12481 atttgaaatg ccagagatag aaattgaaat ttcagatatt gcttttccta acaattgggg
    12541 tgtcgggaaa aaagaaaatt ttgttgtttt tgttcctggt gctacttata aagatgttgt
    12601 aaaagtaaga ataaaaaaag gagagaaaag tttttcttat ggagaggtgg tagaaatttt
    12661 aagtccctct ccatatagaa aacaagcaga atgtcctcat tttggtattt gtggtggatg
    12721 ttcttttcaa aatttacaat atcaaataca actttcttta aaatttaatt atataaagaa
    12781 aacacttgaa aaaataggag gtatagatat aggaaaagga gaaatttcag aaatagtccc
    12841 ttctccttgt ctttattttt acaggaataa aatggaattt tcttttggtg gggataagga
    12901 aatttattta ggtttgagag aaagaaattc tccttttgaa aaatatacat ggaaagttgt
    12961 atcaattgag aaatgtttaa ttttcagccc tctttatgaa aaaatatcac caataattat
    13021 tgaatttgca aaaaaaacag gactcccacc ttataatcca tacaaaaaag agggattttt
    13081 cagacattta gttttaagag aggggaaaaa tacaggtgaa gcaatggcaa ttattgttac
    13141 aaagagtggg aaagacattg atttcaccgg acttgttgaa aaaataaatg atgttgcccc
    13201 tgaaattaaa tgtctttact gggttgaaaa tgaccagtta tcagatgttg tctcttttga
    13261 gaaaaaacac catatctggg gagaaacaaa aatagaagaa aaaataggcg attttaaatt
    13321 taaaattgcc cctaattcat ttttccagcc aaattcaaaa ggggcagaac ttctttattc
    13381 agaaatttat aataatattg gagaagggga gagagtgtta ggactttatt gtggaacagg
    13441 aacaattgaa gtttttattt caaataaggc aggtgaaatt ataggaattg attcagaaga
    13501 gataaatata ttagttgcag aagagaactg tcagaataat aaaattaaaa attgtaaatt
    13561 ttattgtgga agggttgaaa aaattattgg taataagaat ttagggaatt ttgatacttt
    13621 aattgttgac cctccaagaa gtggtttaac aaataaagca ctaatgaaat tggtaaaaat
    13681 ggaaatacca aaacttatat atgtttcctg caatgtttca actttctcaa gggatataaa
    13741 tttattgtca aaggaaggat ataaacttga aaaattaatt ccttttgacc tttttcctca
    13801 tacgccacac atagaaattc taggaataat caagaaacgg agtccccttt gttaacttac
    13861 gaagttaaca agtatatgag aatcaacaac ttacaaatat tactgcaact tttttaagaa
    13921 agaaccttat tttttcccca atataataaa tttggcgtcc atcccactca ataggcattt
    13981 ttgatagtgc ttcaataatt ttatccaata aatttcctga tattatcatt tagtattgaa
    14041 ttattatgta gacagttaag tactgatgca aactctggaa aaagtttttg atttatttct
    14101 tctatcaaag caatattatg ttttacattc tcactacttt tcccggcatt ttttttgaca
    14161 accctgttag ttaattcgga attctcaatt tttgatataa gaattgggtc tgctgaaata
    14221 ttatctggaa ttggtagcga aagcagagtt ttttttgaaa tagtcatacc cccatcatta
    14281 attcgccacc accaataaaa gaaactgcta tttagaagta tatatgcaag attataatct
    14341 tttttgttat aaaaatataa agttctaaat gaggaacgat taacttctgt tttaagtgct
    14401 gaaataaaat atcttggagt tgttggaaca ataagtttat attttgttgg ataagttgaa
    14461 ataatatcta ccaaacattt ttttgtcttt tttactttat agtataaagg taagagttct
    14521 ttttgaattt tgggaaaaac atctgcatta actcctacat cggttaggta attatctaaa
    14581 ctttccagca tttttgtgcg ttccgttgtc cgccaacgta atagaggcgt aattttaaaa
    14641 gaatttaatg tactttcgga tttagcaaca gtgattgcag ctcttatact atttgattta
    14701 ttggtattag ttgaaccaaa tttaattcct ctaaatattg tatcaggaac attatcaaaa
    14761 caataaattg aaatgtttcg catttttgtt ataagtaatt cacgcaaagt tcggaattgt
    14821 tgtccatttg taaatgtctg tggcgtaata gaaataaatc catttgatag agtaattatc
    14881 ttttctaaaa agtaagcata taaattgcgt gcttctgctg ttttaaagcg gttatcactt
    14941 tttacttcta catagggtgg atttaaaaaa gcgaaatcaa atgctggtag atttgaagag
    15001 aaaagaaaat cagtaacaat aaaatgagaa gcaatatcat gaaatagatt ttttgactta
    15061 ttttgaaatg ccagggtgaa taaagtacga gcaataaata atgctaaagc atctttatca
    15121 atcaaataaa gttgattggt taaaaaatcc tcacccgttt cctgcaattt aaccagccaa
    15181 aaagaaagat tgccaacccc agaacaaggg tctatccaaa ctctattttt tgggaaaaaa
    15241 agtgattttt ctgccattac ctgagcaaca tcctcaggag taaaatattg tccatgtttt
    15301 tttctactat cattatttaa aagagacaaa gagtattcat aaagtgtcgc aatttctcca
    15361 atagaaatag aattaagtat atctatatct aaaattggtg cattagaaaa ctctttttgc
    15421 actaaaagat aatcaaaacg aggttgatga agattataaa tatgtgtggc taaaattgaa
    15481 taccatgtct tttcaagacc aaatttattt ataaggatat ttaatttttc atatctaaac
    15541 tttgaatata ttttatcttt tgttttatat aaattctctt gttttgtaat tactagtgat
    15601 aaatttggga ataaataagg gcaatctaca tttgctccaa attctatctt tctctgattt
    15661 atttgaactt ttgccattat attcttactt cctattattt taaacaaatt ttagaaattt
    15721 ctatatatat aattcaaatt atttacttgt ttctatcaaa ttcacttcac atttaaggga
    15781 tgatttttat ttcactttct attgatgcct ttttatattc tgaaataatt tcaagaaatt
    15841 tctcaattgg taaattttgt aatttgtttt tataaatatt gacaaaatta agcagtcctt
    15901 cagcagaaag aaatccctga tttaactcaa aatacaatgt ttcaaaatcc gtcttagaaa
    15961 ttgatgaaag tttatttatt tcacaataca ttttttcttt tttcttttcc tctattatca
    16021 tttcaaactc ttctttaagt gctcgttctg cttttactcc aaattcatat tctttgattt
    16081 catcgggtaa atttttaatt ttaatttctc ttaatttttt ttcttttttt acatatatat
    16141 tccctaataa aattggtata aatattaaaa gaaatccaaa ataaagtcct gtccctaaaa
    16201 cccctattcc catagcaagt gcaggctctc cataatatac atttttaata aaatcaataa
    16261 aaccggtaaa aataccatat ctcaatttaa aaatccagga atataaagta tcaacaaaaa
    16321 aaagagcaat tcctaaaagg acacccctta tatacccata gtcctgtgca ttttcttcta
    16381 caagtgttaa aggaataaaa aataatttct tttttacagg gaaaaatgtt tttgtgacta
    16441 tttcaaggtt ttcttttaat ttttgaatgt tttcaaaaga accataaaca attattgaat
    16501 tttctttgtt taaataaatt gcaacaaatt tatttactgg tgaggaataa tttgtaacga
    16561 aatatgggaa aataaaattt ctctttgtat atatttccca ttttccattc tctaattcaa
    16621 atgcatcttc tcttttatat ccgagccaac caagtaattg atatgtttga caacctactc
    16681 ttttaaaata ttttatctct tcgtatattc cactttccac aatataattt tacaacttaa
    16741 tttctataag taaaattttc tgaccaatga ttaaaagaaa atgcctaaca tttaatttga
    16801 caaatatgct ttaaaaaagg tataatgtag agttatagga ggataattat ggatgatata
    16861 aaaaacaaat atgatgcgac aacaatacag gttttaagtg gaattgaagc agtcagaaaa
    16921 agacctgcta tgtatattgg agacacttca attggcggac ttcatcatct tgtatatgaa
    16981 gtagttgata atagcataga tgaggcaatg gcaggttatt gtactaaaat aaaaataact
    17041 atccattctg ataatagtgt tacagtggaa gataatggaa gagggatacc cattgatatg
    17101 cataaaacag aaaaaaaacc agcagttgaa gttgttatga cagttcttca tgctggtgga
    17161 aagtttgata gaaaagttta tagtgtttcc gggggactgc atggagtagg tgtttcagtt
    17221 gtaaatgcgt tatctgaata tttagaagtt gaaattaaaa gagatggtaa tatttatcat
    17281 caaagatatg aaaaaggtat tcctgtaaca caattaaagg taattggaaa aacaaaaaca
    17341 acaggaacaa aggttacatt taaacccgac tctaaaatct ttgaagtagt tgaatttgat
    17401 gagaatatac ttactggaag gtttaaagaa cttgctttct taaatgcagg gcttgagatt
    17461 atttttgaag atgaaaggaa ggcactaaaa gaaaggtata aatttgaagg tggtattgtt
    17521 tcttttgttc agtttttaaa taaaaacaaa aaagttcttt ttgaaaaacc attttattgt
    17581 aaaaaagttg aagaagatgt tgaatgtgaa ttttctttcc agtataatga tggatatagt
    17641 gatacagtcc tttcttttgc aaataatgtt aatacaaaag agggtggcac tcatcttaca
    17701 gggtttaaaa gtggtttaac aaaagcaata aacgaatatg gaaaaaataa tggatttctt
    17761 gataaaataa gtttgagtgg tgaagatgta agagaaggat tgactgctgt aataagtgtt
    17821 aaacttcctg aaccacaatt tgaagggcaa acaaaaacaa aacttggaaa ttcaaaaata
    17881 aggtttattg tagatagtat tgctttcaat caaattttta catttttaga agaaaaccct
    17941 gaaccagcaa gggcaatact gaataaatgt atcacaacag caaaagcaag agaagcagca
    18001 agaaaagcca gagatattac aagaaaaaaa cttgcagaaa caggggcatt accaggaaaa
    18061 cttgctgatt gttcttcaaa agaccctgaa gtcagagaac tttttattgt tgagggagat
    18121 tcagcaggtg gaagtgcaaa acagggtaga gacagaaact ttcaagcaat acttcctcta
    18181 aaagggaaaa ttataaatac tgagaaagca gcaattgaca aggtcttaaa taacgaagaa
    18241 ataaaaatga tgataagtgc aattggaggt ggagttggag atgaatttga tgtaacaaga
    18301 gcaagatata aaaaaataat tattatgact gatgcagatg ttgatggttc tcatataaga
    18361 acattacttt tgactttttt ttatagacaa atgactgacc taataaaaca tggatatatt
    18421 tatattgctc aaccacctct ttacaaagta aagaaaggta aaaaagaaat ttatgttgaa
    18481 acagagaaag aaatggataa tattcttatt gattttgcaa cagaaaatgc aaaaatgaaa
    18541 atttcttatg attcaaaata tattgaaatt gttgaggaga aagttagaga aattctatct
    18601 cttagcaaaa atttaaatga gttaataaga acaattgaaa ataaaggaat aaaatttaaa
    18661 aatttaattg attattacca gaaaaacaaa aaaatcccac aatattattt agtagataat
    18721 gtagataaaa aagagtacta ttttgataac tataaacaac tatctgaatt tcttggtaaa
    18781 aaagatgaag aaattgacct attttctgac gaaaagtcaa actataaaat tgtagaaata
    18841 tatgaaaaag gacaacttga aaaaattctt gagaaaatta aggaatttaa aatagcacca
    18901 tctgaaattt ttaacaaatg ttttatccta ataaatgagg aagaagaaag gtattcttta
    18961 atagagatat ttgaagcatt aaaagagaaa gggaaaaaga atttatttat tcaaaggtat
    19021 aaaggacttg gagaaatgaa tcccttacaa ctatgggaaa caacgatgtc tcctgaaata
    19081 aggactttaa aaaaagttac aattgaagat gcaattaaag cagaagagat atttacaaca
    19141 ttaatgggag atgctgttga accaagaaag gaatttatag aaaaatttgc aaaagaagtt
    19201 aaaaatcttg atatataagg aaaagaaatg gaagagaaaa aaattataaa tattggtgtt
    19261 gaagaagaaa tgaggcagtc ctatctggac tatgctatga gtgttattgt tggtagagca
    19321 ttacctgatg taagagatgg attaaaacca gttcatagaa ggattcttta tggaatgctt
    19381 gaattagggc ttgaaccagg aaaaccattt aaaaaatcag caagagtagt tggagaagta
    19441 atgggtaaat ttcatccaca tggtgatgca gcaatttatg aagcaatcgc aagaatgaca
    19501 caaccattta ctttaagata tccaattata gagggacagg gtaattttgg ttcaattgat
    19561 ggagacccac cagccgccat gcgatatacc gaagttcgac ttgcaccaat tgcgatggaa
    19621 atacttactg acattgataa agacacagtg gattttgtcc caaactttga tgaatcatta
    19681 gtagagccag ttgttttacc aagtaaagtt ccacatcttc taataaatgg ttcttctgga
    19741 attgctgttg gtatggctac aaatatacca ccacacaata ttggagaagt acttaatgct
    19801 ttgatatttt tgttaaagaa agaaaatgcc acaattgaag aaataatgga aatattacct
    19861 ggacctgatt tcccaactgg aggaataatt tgtggcaaag aagggataat taaagcgtat
    19921 aaaacaggaa aaggaataat aacaataaga ggagaaattg aaattgaaga agacgacaaa
    19981 ggcaaaaaga gcatcataat aaaaactata ccctatgaag ttaataaggc atctcttgtt
    20041 gaacaaatag cacatcttgc agaaatagaa agaatagaag gagtttctga aataagagat
    20101 gaatcggata aagaaggaat gagaattgta attgaactta aaaaaggaga aaatgaagaa
    20161 attgttatta atcaacttta taaaaatact caacttcaga caacatatgg aattataaat
    20221 cttgctttat caaatcaagt tccgcgtctt ttaaatataa aacagatgct ttctcttttt
    20281 cttgaccata gaaaagaagt tattattaga aggtcaaatt ttctattaaa aaaagcagaa
    20341 agtagatatc acatcttact cggacttata attgcccttg ataacttaga tgctgttatt
    20401 cagttaataa gaaaggcaaa agatgttgaa agcgcgagag atgggctgat aaggagtttc
    20461 aaaattgatg aaaaacaggc aaatgcaatc cttgcaatgt ctctttcccg tttaacaggt
    20521 atggagagag aaaaaattat aacagaaagt aaagaaacag aaaaagaaat agaaaaatta
    20581 aaaggaattc ttgctgaccc tgaaaaagta aagaatataa taaaagaaga gtttgaagaa
    20641 ataaagaaaa aatatgcaga taaaagaagg acagaaatag gtgggccagt tgaagaattt
    20701 gaagatatag accttgtaag agatgaagat ataattgtaa ctgtttcaaa tgatggatat
    20761 ataaagagga gttcacttga atattacaaa agacagagaa gaggaggaaa gggttttata
    20821 ggagcaaaaa caaaagaaga tgactatgta aaacaccttt ttgtaacttc aacattagat
    20881 acaatacttt tcttttctga aaaagggaag gtgtactggt tgaggggcta tttaatccca
    20941 gaaggaagta gaacttcaaa gggaaaacca attgttaatc tccttaaaat agacccagga
    21001 gaaaaaatta ctgcaactat tcctgttaag gaattttcaa gtgataaata tcttgtaatg
    21061 ataacaaaga aaggacttgt taaaaaaaca ccattgattg cttatagtag accaagaaaa
    21121 ggaggaataa tagggataaa tttaagagaa ggagatagat taatagatgt taaagtaacc
    21181 gatgggaatc aaaatatact tcttgcaaca aaatatggtt tttgtatcag atttagtgaa
    21241 aaagaagtaa gaaatgtagg aagaagttca ataggagtaa caggaataag atttaaaaaa
    21301 gaaaatgatg aggttgttgc aggagagatt ttttctgaaa atgaattgga cctttctctt
    21361 cttacagtaa caagtaaggg atatggtaaa agaacaatac tttctcgcta tagaaaacaa
    21421 aaaagaggag gtaaaggaat tattgatata aaagttaatg aaaaaatagg gactattgtt
    21481 ggggtgaaat gtgttaaact ctctgatgaa gtgattttaa ccaccaaaaa tggtgttgta
    21541 ataagaatgg attgcaagga aataagagca gttggaagaa atacaatggg agtaaaacta
    21601 ataaacttag gcagtaatga tgaaatagca gatattgctc ttgttgaaaa aaatgagcag
    21661 gtataagata attgaccata cagcagatat tggaattgaa gttgaaggtg actcattaga
    21721 agaaattttt tttcaaagtt ataaagggct atatgaaatt gcaggaatta aaaaagaaac
    21781 aacaggtgaa aaaggaaaat ttgaaattaa ggcagatacc ttagaagaat tattgataaa
    21841 atttttaaat gagttaatat attatatcta tgtaaaaaac aaattctggg agataaaaaa
    21901 attggaaata aaaatgaata aggaacttaa aataaaaata gaagggaatt caggtaaaat
    21961 aaataagttt gaaagagaaa taaaagcagc aacatatcat aatatatcta taaaaaaaga
    22021 aaatggtatt tttaaaacaa caattatatt tgatttataa ataaaagaca caatgttttt
    22081 tggcgaattt tttggcggag agacagaggg gggattttat ccttctcccc agcggggaga
    22141 aggtgggatg agggggttga tatttccccc tttgataaac cgacgaaagt tgcttacctt
    22201 gtttctcaga gttggtgcgt tatcgtttcc cagatagcac aactgctacc gactaaggaa
    22261 gcaaaggtta gcagagcaga gttggtgcgt catttccccc tttaaaaaag gggggggagg
    22321 gggatttcca gatagcacaa caggaaaatg gggaggattt tagttctctc tatttgctac
    22381 taaaatattt ataaatatgt gaggtgaatt acaaaatgga taaaaaagaa aaagaatatt
    22441 atttaaaaat tttattaaaa gagaaaaaca caatacttaa aaatttagga tatttgaagg
    22501 aaggtattga aagtgttgaa aaaggcgtcc ctactcatat tggtgacctt gggacagatg
    22561 agtttgtgag aggacttggt attgatattt ctcactcaga agagcaaatt ttaaggcaga
    22621 tagatgaagc aatagaaaaa ttagaaaaag ggaaatatgg aatatgtgaa aattgtggga
    22681 agaaaattcc aaaaaacaga ttaaaagtaa taccatatgc aaaatactgt gtaaaatgca
    22741 aaaaagagat ggaaaaaagt gaataaaaaa tccattatta ttttcttttt ggtctttttc
    22801 atttcactca ttcttgacca gttctcaaaa acacttgttc tggaaattat taaagatgga
    22861 gaagtaaaaa tcacaaaata tttttctctt atttttgttg gaaataaagg tgttttattt
    22921 ggattattca gtaatttgcc catgaatatt ccaataatta tcatttcctt ttttgttatt
    22981 ctaattttat gtatttatgc tataaattca agtaaagaaa gcaaaattct tcatcttttt
    23041 cttggattag ttgcaggtgg aattggcgga aatcttcttg ataggattaa atatgggtat
    23101 gtaattgatt tcctggactt taaaatttgg cctgtcttta atttaagcga tgtatttatt
    23161 gtaagtggaa caattttgac aattctttat tacattaaaa gaaaaaaatg catcccgaat
    23221 tcataaaaat agggaacttt gtaatttatt ggtatggggt aatggtcgca attggtatat
    23281 ttgtaagttg ttttattttc caaaaaatta gtttgaagaa tggatattct gaaaatataa
    23341 cttctaaatt attattatgg ttaattttct ggtcaataat aggagcaaga gtactacata
    23401 ttttagttca ttttccatat tactacgggc atccacttga cataataaaa ataagaaatg
    23461 gtggacttgc aattgaaggt ggaattatag caggcctttt gtttattgtt attttcggta
    23521 aaataaacca cctgaaaaca attgaaattc ttgatttagt tgcacttgtt gttccattag
    23581 gacaggcaat aggaagaatt ggttgtttct taaatgggtg ttgttatggg aaaaaaacag
    23641 attttatttt tggagttaaa tttccacatc tatcctataa agttcaccca acacaacttt
    23701 attattcttt ctcctatttc atctttttcc tgtttctttg gttactttat aagaaaaaag
    23761 taaaggatgg tattatattt tcttcatatc ttctattctt ttctctttta agatatctta
    23821 tggacttttt aagaggagat ttaaaaggaa catttctttt tctatatcca acacaactaa
    23881 ttgcaattcc aatatttttc ttaggtggaa ttttatttat gcttttctta cagagaaagg
    23941 agaatatgga tggcgaaatt ggtgtttaat tatttattta tgagaaaaga gtatgacctt
    24001 gaaaattttg ggaaaaatga aatttatatt ggaagaatgg atagtaatga tattaccata
    24061 cctgactatg ttttatttaa aaaattacca ttagagacac aaaaattata tataaaggac
    24121 ttagtaaaaa tttcaagaat ccattcaaaa ttaataaaaa ttgggaataa ctggtttata
    24181 gaagacatcg gtacaaaagg acttggaagt aattttggca catatgtaaa tgatgcccgc
    24241 cttgaagttc aaaaaccata taaattagaa aataatgaca aaataaggtt tggtcctttt
    24301 atttgtactt ttgtagaaga ataaagtaaa tagtattttt attgttataa aattttaatt
    24361 ttgacttata acttcttttt ctatgcttat gttgttgaat ttctggttga gaatacagcc
    24421 ggggtcaggt gccaatatat caccaaaata tgcatatgtt cttgctgcac aaccaccaca
    24481 aactgactta tattttcctt tacatccata attttctgaa taacttcttc tcattcttaa
    24541 cttatttata atttctgctt tatcccacac atcaattaaa ttatctattt ttatctcacc
    24601 atctttcttc atattatccc aaattccgcc ataattaggc actaagtaag tttattatat
    24661 tatatattca tcacagcaac agggtttttt aacaaaggta gatgctatac ttaatgccta
    24721 tatgaacatt ataagaagaa aatatgtctt tatccccatt tgagtttata catacagtaa
    24781 aaacatttcc actcacctta aatccctcgg gcgtagtcca caggctcgcc cgttggggta
    24841 tgccctgagg ggatccgtaa gccaaaagcg aggaccctac gggcgagcct ctccccagtg
    24901 gggagagagc aggggtgagg gggtattaat ttttagtata catttgatgt aaaaacaata
    24961 tgaatatctt agtgtccaaa aatatggggg aatttaggat tatactccta ttttatgaaa
    25021 ttctttttga agaagttgca ataaattaaa aattgtgata caataaaaat atgaaaataa
    25081 atgagtttta taatggtctt gataaaagga aaaaaataat attgtggatt gctattgctt
    25141 ttgtcttttt ttcaatttta agggtttcta tatggaaagc aaaaagtacc aatattccaa
    25201 ccgaaaagaa aatttgcaag caaatttatc ttcctgataa aaagaaaaaa atagaaggaa
    25261 tatatgctat tggtcaattt aaaaaagaag agtatttacc agaagtagaa aaaattttta
    25321 atagtacaga tgatgttgaa attaaaagag ttgctgcttg gacaataggc aaaattgata
    25381 tagataaact aaaaggttat ttggatagtc aaaataaaaa agaagataaa atactggtta
    25441 ttgatacact tttaaaaatt gataaaaaca atattaattt tcttatcgag agatttccaa
    25501 aagaagacaa ggaaacaaaa ttatacataa tggacattat agagaaagag gaatattctg
    25561 ataaacttat ggaaattgca gaaaataaag atgaagaaaa agaaataagg atgaaggcat
    25621 taagtatgtt agaaaaatta ggaaataatg agcatttttc taaattatgg aatttatact
    25681 ataatgatac tgacactgaa atacagatgg catcttatag gacaataaaa gaaattgaaa
    25741 aaagaggtac aaaatgaaaa accagaaagg cgggtttact ttaattgaat tgcttgttgt
    25801 aattgcaatt atatccctgc ttgctggaca acttcttcct tcactatctt ctgcaagaga
    25861 gaaaggaagg caagcaaatt gtataaataa cttacatcaa ataggacttg caattgaaat
    25921 gtattatcag gattataatg attatccaac atggttatca attctgtata aaaattattt
    25981 aggaacacca aaaattttct tatgtctcac tgatttatac aaaggggaaa agggctgtgg
    26041 aaattccctt cacccatcta taaatgatat tcctctttca caaattccta actatacacc
    26101 tgatgatttt gattcaactg cttatagtta taggaatcct aatataacat tctgtagtta
    26161 ttcatatgaa tttgcaccaa ctaaaagtga atggtttatg gcagcacata cctcagggga
    26221 acaacaagag gcagatataa atagtgatgg cgttgttaca tggaaagaag caagaatatg
    26281 gcaatcaaat catggatatg gagggcaggt accaattgta agatgttact ggcattttta
    26341 taattatggt caaaaggtaa taaatcttgc tttccgcgat tacaatgtat attcaagtgg
    26401 ggaggagtgg gaaacatctt cttattagtc cttttgcaaa ataatacagg aaaaatataa
    26461 agtggaaaaa aaaggtgttg attttataag agagataatt ttaaaagata tagaagaagg
    26521 aagatatagt aaaaaagttc atacgcgatt tcctccagaa ccaaatggat accttcatat
    26581 aggacatgca aaagcacttc atataaacta tacaattgca aaggaatttg gaggaaaatt
    26641 taatttaagg atggatgata ctgacccaga aaaagaaaca aaagaatatg ttgatgcaat
    26701 tatagacaat gtaaagtact tagggtatga ctggggagag aaagtatatt atgcttctga
    26761 ttattttgat aaattctatg aatacgcaat ccaactaata gaaaaaggga aagcatatgt
    26821 ttgtgaccta ccacctgaaa aattcaaaga atatcgggga actttaactc aaccaggtaa
    26881 agaaagtcct tatagagata gaaccattga agaaaataaa gttttatttg agaaaatgag
    26941 aaatggagaa ttccatgaag gaaagcgtat attgagagca aaaattgata tgaaaaaccc
    27001 aaatatactt atgagagacc ctgttatata cagaattaaa aaaacacctc attacaggca
    27061 gggaaataaa tggtgtatat atccaacata tgattttgct cattgtttgg aggactcaat
    27121 agaaggtata actcattcat tatgcacaat agaatttgaa attcatcgtc ccttatatga
    27181 ttggtttttg gaacaacttt caatacatca tcctaaacag attgaattcg cccctttaaa
    27241 tttaacatat actattttaa gtaaaaggaa attaactgaa cttgttgaaa aaaaatatgt
    27301 aaatggatgg gatgacccaa gaatgccaac tatttccgga atgagaagaa gaggatatac
    27361 tcctttaagt ataaaaaatt tttgtaatga aataggaata tcaaaaacag aatcattagt
    27421 taatattgaa cttcttcatt attttgtgag acaacactta aataaaatat gcccaagggt
    27481 tatggttgtt ttaaatccaa taaaaattgt aattactaat tttccagaag ggaaagttga
    27541 atatcttgat gcaataaata atccagagga cgaaaattcc gggataagga aagtcccttt
    27601 tacaagagaa attttcatag aaaaagatga ttttatggaa gtacctgcac aaaaattcta
    27661 tcgtctttcc cctggtagag aggttagatt aagatatgca tattttataa aatgtgaaaa
    27721 aattataaaa aacgaaagag gggaaataaa ggaaatacat tgtacttacg acccagatac
    27781 aaaaggggga aatgctcctg atggaagaaa agttaaagca actttacatt gggtttcatc
    27841 ttcaataaat acctctgttg aagttcgcct ttatgaccat ttatttacaa aagaaaatat
    27901 gaatgaaata gaagaaggga aaacatttct tgattatttt aacaataatt cattaaaaat
    27961 tttgagaaat tgccctgctg aaataaattt aaaagatgta aaagaaggag agaaatacca
    28021 atttgaaaga gttggttatt tttgtgttga ccctgatact gaaaaaggta ataatgtgtt
    28081 taatcttaca gtaaaactta aagacgaatg ggagaaaatt aaaaaagggg gtaattaata
    28141 tggaaattaa atttacaaaa gaacagtatg aaaatttagt aaaattagta tatttaggta
    28201 attggatgat taatgcaatt cgtcagggtt ctaacatcat tgagaagttt gaggacctag
    28261 agcagtatat cttttcattt tataatgaat ttggccttga aggttgtatt gaatatgatg
    28321 aagaagataa aaggttttac ccaacaggaa aatttgaaat ggaatccgaa atagaagaat
    28381 atagacagga atatgataat gagaatttct gggatgaatt aataagcaga ttggcaagaa
    28441 gagattttat tagaaagtat ggggaagatg ctataaataa aatgacttta gaagaaataa
    28501 tggaaaaaga aagtcatttt attgaaaaat atgaagaaga atttgaaaaa tatggaattg
    28561 aaagattaga gataaaaaaa tgagtgaaga tatttataaa cagcaggaag aacaattaaa
    28621 gacaaaagat atttttgatt ctgtttttaa aaatactgat acaaaataca gtttacagga
    28681 attttctcaa aaaattttag gtagaattac tacttttgaa aaagaacctg gcaggtatta
    28741 tattcgctgc ttaaaaagag ataaagacat tttcatttat gataaaaata aaaattttgg
    28801 caagccagaa gaaattattc gtcaactctg gttaataaaa ttaacacagg attatggata
    28861 cccattagat agaatagaag tagaaaaatc agtacaattt gggcgggaag ttaaaacaaa
    28921 agcagcagat attgttattt acaaagagga taaaattaca ccatatataa tttttgaaat
    28981 aaaagaacca aatgttaaaa aggcagaaga acagttaaaa tcttacttaa gtgcagaagg
    29041 ggcagaagca ggagtatggt ctaatggtat ccagaaattt attctttatc gtccctatcc
    29101 gaaggaatac ataacaacct ttagagatat accaaatgtt aatcaaacat gggatgacct
    29161 ttttgaagaa aaaaggactt atgagaaatt gcaggaaaaa tttgatttaa catttataat
    29221 ttcacgactt gaagaaatgg ttttagccca agcaggtgta aatgtttttg aagaagtttt
    29281 taaactaata tatgcaaaac tttatgatga ggaccaggca aagaatttca ggaaagaaaa
    29341 agaaatccat ttcaaacaat ataaagagcc aaaaaaaaca tatgccataa ttaatgatga
    29401 actttttaaa ggagcaatta aagaatggcc cgatattttt aatccaatag aaaaaatcaa
    29461 tcttactcct gaacaattac aaatctgtgt tccgttttta gaagaaatta aactttttgg
    29521 ttcaggcaga gaagaattgg aaataattga ccgtgctttt gaacacttaa ttgctgatgt
    29581 ctccaaagga caaaaaggac aatattttac tccaagaaat gtaattaaaa tgtgtgtaaa
    29641 aatgcttaat ccaaaagaaa atgagtatat tattgaccct gcctgtggtt ctggtggttt
    29701 tttacttcat acaatgtatt gggtgtggga taactattta agagatacat ctttagaagt
    29761 aaaaagaaga tatgccaatc gttatttatt tggtattgac tttgatgata atatgcgtcg
    29821 tatttcccag gcgttgatgc taattgcggg ggatgggaga cataatattt tcaaacgaaa
    29881 ttcattagat agtagagatt ggattggtga acaagcagaa gaagcaagag caagtttaaa
    29941 accattatta tctaaacaga atgaagaaaa tttaaaaaca tatcgttatc tcaactttga
    30001 tttattaatg actaatccac cttttgctgg tgagattact gattctggat tattacgaca
    30061 atatgaattt gctaaaaaaa atgggaaact aaaacagaaa gtagaaaggc atcttctttt
    30121 tattgagcgt tctttagacg ctcttcgtcc aggtgggaga atggctattg tattacctca
    30181 gggtatcttt aacaatacca atatggaatg ggtacgagaa tggttttttg aaaaagcaag
    30241 aattttagca atagtagggc ttgaaggcaa tacatttaaa cttccatctc ctgcaaaagg
    30301 cacaggcact aaaacatcag tattgttttt gcagaaatgg gaaaaagagc aaagaccatt
    30361 agaggattat catattttta tggctgtttc taaaaagtca ggaaaggata attcaggaga
    30421 atatattttt gtaaatgaaa aaggacaaag agtatcctct aaaaatggtt tttttaagtc
    30481 acaggagaat ttaaaattgg atgatgattt agatgaaatt gctgaagaat tcataaaatt
    30541 tgcaaaaaaa gaaaaatttg aatggtggat ataaaatggc tgtctactca ataattaaaa
    30601 aatctcaact tgaaggaacg catagattag atgctgagta ttatcaacca gagtatttag
    30661 agataaatcg aaaaattagc cattttaata atttcttctt aggtgatcta atccattctt
    30721 ttgcttctgg gaaaaatttg cctcaagctg ataatggtaa aatatttttt ttaagaactc
    30781 aaaatatagg ccgagtttta attaataaag aaggtatgaa ttttctcgct ccaaacattg
    30841 aaaaatatcc cgttcttaat gaaggagaca tcttagcagt gagggttggg gagggtgtag
    30901 gtaatacttc aatagttact cctaaatttg caaagcatag ttatagtgat aacgtaatta
    30961 gatttcgcat tgagaaatta gatccctatt tcattataat ttttcttaat tccagaatcg
    31021 gttttaatta tttagaacga gtaagcaaag gtagtgcgcg ttccctaata agctctgaaa
    31081 acatttatag tattagagtt cctgacttag ggcaaaaagt gtctaatttt tgcaagaaag
    31141 cgatattaga aggggcgaaa gaagttgaac aatccgaatc cctttactta caggcagaag
    31201 atttactttt agaagaattg gacctaaaaa attttcaacc agaagatgat ttatcttata
    31261 tcgtcaatct ttctgatgta aaaaaagtcc atcgtgctga tgctgagtat tttcaaccaa
    31321 agtatgaaaa gttagttcag aaaattaaag agaataaaat agaacattta ggtaatttgg
    31381 tagttatgaa aaaaggaatt gaaccgggaa gtaatgaata tcaaaatgaa ggcaaacttt
    31441 tcattcgtgt aagtaatatg tcaaagtatg aaattactga taaaggacaa aaatatttaa
    31501 gtaatgaatt ataccaaaaa cttaaagata attttgagcc aaaaattgga gagattttgt
    31561 tgactaaaga tgcaacacct ggaatagcat atgttatgaa agaaccaatt gaaggaatta
    31621 tatcaagtgg aattttacga ctggaattaa aagaaaaaga tattgaagaa gaatatcttg
    31681 cattatgtct taattctatt attgggcaga tgcaggcaga acgagatgga ggtggttcta
    31741 ttattactca ttggaaacca gagcaaataa aaaatttaca aattccgata ttacctaaaa
    31801 gcactcagca aaaaataacc gatttagtta gaaaatcaca ccaggcaaga aaacaggcaa
    31861 aacaattatt agaacaggca aaacaaaagg tagaagaatt aattgagaat tcacaaaatt
    31921 tttgacttat tcagagattc ttgttttgat atagaaagga aaattgcttt ttgaaggaat
    31981 atattataat atttgctata aataaaattt gaggtataat taaaaaagtt aggagcaaaa
    32041 aaaatgacaa agaaaaaata taaagaccct gtttgtagaa aatatcttgc gaaagaaacg
    32101 aagaatattg ttgagaaaga tggaaaagtt tattattttt gttcacctca ttgtaaagaa
    32161 aaatttgaaa aggactctga aaagtactta aaattggttg gttaaatgga aaaagtaatt
    32221 ccgcctttaa tagcacttga tgttgaaaca actggactaa aaccagatga aggtgataga
    32281 atagttgaaa ttgccttgat taagtttgat gaaaaaggta aaattattga taaattcatt
    32341 tctcttataa atcctgaaag agaaatacca ttagaagtta caaagtttat caatagaata
    32401 gataaaaata tggttgaaaa agcaccaaaa tttaaagaag ttgcaaaaga gattaaaaat
    32461 tttatagatg gtggaatagt tgttattcac aatgctgatt ttgatatttc atttctaaaa
    32521 aaagaatttc aattatgcga agaagaatta cctgaattcg gagttattga tactctttat
    32581 atcgcaagaa aatattttaa ttttccaagt aattctcttt cttcacttgc caaaaattta
    32641 aaaattgaaa aagaacctca tagagcagaa agtgatgcaa taactgctta tgaaattttt
    32701 aatcactttg ttaaaaaaat tgatgttgaa atagatgacc ttatttgttc ttttggggaa
    32761 atataaaaat aaattaaatt tgtcttgaca taactgaaaa tttatgaata attataagat
    32821 agaggagaaa ggatgaggaa gttgattttt gttttagtat ttgtgggaat agttgggtta
    32881 ttttctggtt gtactctcag cttggatttc catcctgaag gtaaaaattc agaaacaaca
    32941 aagtaaatta aataaactta attattaccc ccaaaatcca tttcaaagaa attggaatta
    33001 ttggggaaag aaaggaggga agtaaagtgg caagagaaaa aggaaaagtg aagtggttca
    33061 ataattccaa aggttatggt tttattgaac gagaaaatgg cgaagatgtt tttgtccatt
    33121 attccgccat tgaaggagac ggttatcgca ctctcaatga aggtgatagt gttgagtttg
    33181 atatcgtcaa gacagacaaa ggttttcaag cagcacatgt gaaaaaagca taaaaaagaa
    33241 aaagatatgg ttgccgggta aaacacccgg caactatcta attctcataa acattagatg
    33301 aaagttgctg tgaatgaaaa agtggattat tttatttttg tctgctggta ttttatatgg
    33361 agaagttgat tatttgaata tggggcttgt tgctcaccaa aaaggacttt ataatctttc
    33421 caatacacaa cttgaaaaat atattcaaca aaatccagat ggtcaatatc ttgattatgc
    33481 ataccttctt tatgcaaaaa atttaataaa cctcggaaaa tttgaagaaa gcaaggaaaa
    33541 attaaccttt ttaattgaaa attttcccca atcacaatat ctaaaagatg cttatacata
    33601 cacaattctt gtttgtcttg aactttctga attaaacaca tcattagaat tttataatgt
    33661 atatattaaa aaatggggag aaaataaaga aattgaaaat caagtgtgtg gtaaacttat
    33721 acaaagagga atagattctt tccaaaaagg aaactataaa aaagccataa agtgttttaa
    33781 tgaagttctg acaatttcta atgatacatc ttttttagat attgctaact attatactgc
    33841 tttaatatat tatcagaaaa atgatttttc aaaagctaaa gaattatttt tgaaaattaa
    33901 aggagaaaat agcaagatat tgcctgatgt ttatcttaaa attggtgact gttatcttaa
    33961 tttgaaggac tataatgagg caaaaagata ttacgaaaaa gtagtaagca cctatcctga
    34021 aacagaaaca gcaaattggg cgatgtatca aattgcagta attaaaaaga aagaaggaaa
    34081 attaaatgaa ggtgaattaa tattaaatag aataaaaaca aatgataatg acttaaaatt
    34141 acagatatta caaacacttt ctgaaattta tatattagaa gaaaaatgga ctgatgctga
    34201 aaaaacaatt cttgaaattt ttcagcaatt cccttctgaa aaaaatcctg ccaattatat
    34261 aaaacttgcc tttataaatt tcaatatgaa aaatatggaa aattctattt catacttcaa
    34321 ttcagcactt aatttgaacc cttctgatga attaaaagaa aagatatatt ttggacttgg
    34381 ctatacatac tatttaaacg gagatataga aaagtccttt tctttctggg aaaagataga
    34441 aaaacaattt cctgaaagta gttttttacc agagatatat tatataaaag ggaaaaaata
    34501 ttttgatatg aaagaattta caaaaagtga aaaatatctt aaaaatttag ttgaaaatta
    34561 caaagaaagt tcttttataa ctcaggcaat tccccttctt attcaaatat acatagaaga
    34621 agggaattat aaaaaagcag aagaaatatg tcaggaagtt aaaaataaaa atgaagagat
    34681 taactttctt tatgggaaaa ctctatatct aacaaaagaa tacgaaaaag caaagtatat
    34741 atttgaaaca acagatataa aaaatcctca atttaaagtt gaatccctgt actatcttgc
    34801 tcaaatttat ctttatgaag ggaaagaaga aaaaggtaaa gaaaaattgc ttgaaatagt
    34861 tacttattat cctcaattta aagaatttta tgaaaaagca gagaaacaat taaaaaaata
    34921 tgaaaaatga aaaaattttt tctatttata tttttatcat cttttctttt tgcagaagaa
    34981 ataaaattac cagaagaaaa aatagttggt gaaagtaagg aaataataaa aacaaaaatt
    35041 tttatctttt caccacaaac cgaggaaata actttcccag aaatagaaat gccagaaata
    35101 aaaataccag aaaaagaagt taaaaaagaa aaaaaagaag aaaaaaaaga agaaataata
    35161 aataaaaata aagaaaatag tatttttata tcaggtggga gtttttatac aactgacttt
    35221 aatttttctc ataagggaaa caacatttct tttgaaatat ataacaacta tactgaggga
    35281 tttagagaaa atgatgagga atactcttct ggaataaact tttataaaaa ggaggagaaa
    35341 tatttagttg atattaattt gaaaggtgga aagaaagaat taacaggacc tttatttttc
    35401 cctttcaata tagatagaaa ctggttaaat cttcaaagtg gtttaacttt ctatcctggt
    35461 gaaaaaacat ccatatcaat ttttcaaaat tattataaaa ttgatgattt aaagacatgt
    35521 ttttcatttt tagaaattaa aaatgatttt gattattttt cttcaatttt ttcttttaat
    35581 agacaggact tttttcataa tttttctcaa aattcttttt cagaaggtgt atattctgac
    35641 ataggaaata caaaaatagg agggaaaata aaagttattg aaaattcagg ggtaagattt
    35701 gttccatatt ttaaatcccc tttaattggt ggtctgaatt ttgaaattaa tggaatatat
    35761 agaataccag atttatggga agatgtaatt aaccatacct gggcagagat aaaacaagag
    35821 gaattagaac cagaagaagg atattgggtt gatttaggtt atgaaaatga aaacgataga
    35881 tggcaattaa agacaggtgg aaaatataca gtttggagaa atttttatac atggtctgat
    35941 gttgataata atttcctatt tcaacctttt ccagaaaagt ttaatcaatt tactttatat
    36001 ttgaaccatt catttagttt aacagataat ataaagtatt ttttagaatg tgaaaaagca
    36061 attcgttcaa aagatataaa ttattttcca gaaaattcag ttaattcagg agttatttta
    36121 acctatttac catttattct gaaattatgg actcaataca atggaaaaag agtttcagat
    36181 ggtgaacaat tagacaattt tgttgtttta aattgtgata taaaatataa aaaaggaaat
    36241 taccaatttg gagtgaaggt tgaaaacatt acagataaaa aattttttga tgtaagagga
    36301 tatccaggtg agagaagaaa aacattattc tattttgaat attattttta ggaggaaaaa
    36361 tgagtttgtg ggaactatta cttaaaggtg gagttataat aattccgatt gttttttgtg
    36421 gtgttttaac aataggtata attattgagc gactgatatc tcttaaaaat gcagaaattg
    36481 ataatgaaaa atttataagt gaaattgaaa acactttaag aagaagaaaa ataaaagaag
    36541 caatagacct atgtgaaaaa aatgataacc cagttccaag aattattaaa gcaggacttt
    36601 taaaaattga tagaagtaga gaagaagtta aagaaagtat agaagatgca gcaaatcatg
    36661 aaataccaaa tcttgaaaaa tatttaggaa tacttgcaac aattgcaact gtttcacctc
    36721 ttttaggact gcttgggact gttacaggta tggtaaaggc atttatggta attgaagcaa
    36781 aaggagggct tgttcatcca ggtgaacttg caggaggaat atgggaagca cttcttacaa
    36841 cagttgtagg gcttgttgta gcaatacctg catatcttgc ttataattat tttgtttcaa
    36901 gagtgaacaa tatcgtcctt gatatggaaa aaagcgcaac acgccttatt gatataattt
    36961 ttcttataaa agaagaagat attgcaaaca ggtaaaaata taggtaaata atacctcagt
    37021 gaaacccttt atgcatagac acaaagaaaa caataaaagc ccatcttttt tcttgtaata
    37081 ctaaagaaga aaatgattat tttttgaagg taatatatta aatactttaa atgcaactta
    37141 agagaaaccc tttatttatg tgaattgacg aaaatctaac catgctggtg gtgaaaattt
    37201 ttctcgtacc atctttttaa gattctcatc aatagggata ttttctgttt ttgcaatacg
    37261 cctttcgtgc atttcaaacc atatatcagt aagaatttgt tctccttccc gtatatccgg
    37321 aattgaaggt ctgcttttga gaatttcctc aactcgttcc aaattatttt gtttcagagc
    37381 aacctgtgct tctataattt ttattcttcc ataattgcgt atctcagaag ataaattatt
    37441 tataattttc agtatccctt cgtatttctc cagtttaatt aaaacattac aacactctac
    37501 aattaaatca actacatccg gtgccatttc aactgctttt attaacaaat cacctgcttc
    37561 ctcaatatgg ttattttttc tcgctaatat tcctaaatta cgatatacca aggcagatgg
    37621 ttctcgttcc aatgattttt cccacgagtt ttttgctttt tcaaaattcc cctggctata
    37681 atacataatt ccaagatgta accatgacaa ccagtggtca ttttggggat gtttaactgc
    37741 tttttcaaga atatcttgcc attgtttttg aatcatccat ccgtaaattt tctcttttgg
    37801 agagacataa gggactaaac cgcttatcaa caattcaaga tacatttcct gttctttttt
    37861 caaactaaca tcatcaaata tcaatgcttt tgaacaaaaa gatggttcat tatatttttc
    37921 tcttcttatt cgctctaaag taccccaacc agaacctctc tgtataatct catcaggagt
    37981 tttgtttgcc atttttgttg tctctttaag tattaagtcc aatttatttt ctggcattaa
    38041 acttttaatt tttttatcta ctgaattata tgcctctttc caatttttgc catggactac
    38101 ttctggagta cattccataa gaccgtatgc ttctaaccat gcccattcag caccagcagg
    38161 catctgtata tattccgatt gagtttgagt caaacctgcc tgaatttcaa tataagctga
    38221 cccaggaaca gataaaaatt cctgccagga ccttccacct ttatgcatcc cccatacaaa
    38281 aagttttctt ccataaagtc ttttagtaga tgtttgtata agtccttttc cctctttgtc
    38341 aagagaagtt atccatggtt gtgactctgg tttgatatag taaaagctat ctgctgagtg
    38401 gttgaaatta actgggtaag ataaatcaat accatcaata gccccatcag gagacataga
    38461 aacaatactt ttatttcctt tataatccct tttaagaatt gactcagcag gaacaattac
    38521 gcgtgtatca ggggtttcag gaactgctat attagaccac caatacatag gaacttcatt
    38581 ttcatttgga ttaactattt tcacccgcac aaataggaaa tccgaaccat ctggtagaaa
    38641 agcatctatt tgaaaaggaa ccattcttac tcgttcccat tcatacatcc ttaaaacagg
    38701 agttccatca tccattttta atttacttac ataaagaggt gaacaggtaa aaacagaatg
    38761 tccatggaga cctatgttcc actccacacc accactgaac cacgcatttc ttatggctaa
    38821 attacatggc tgaaaaacag gatttgtata aagtagttct ctctttgctt ttttatgaaa
    38881 tagagaccag agtctaccac caaactctat taaaaaggtc gctttaagta tatcgttttc
    38941 aagaactact gttttaaact cagttatttt cttttctcta ttataattgt tttgaagagt
    39001 atagggtaga catatatcta atggcggtaa tggactatct ggaccaagtt ttgcagcagg
    39061 cataatgtaa ttttctaatc ttaactcact cattttttaa ctccataata gatttttaaa
    39121 ttgaaaaaga aaaatctaaa cattctttca gccattttac acctatcaaa ttcatatttt
    39181 ttatttcttt tacttcttta atgttgcttt caggaataat tgccctttca aatctaagac
    39241 gggagcattc ttttattctt gatattatct gcccaacagg tcttatttca ccagtcaaag
    39301 caatttcacc tataaaaata gtattttcag caggagatac atctttgaaa gaagaaatag
    39361 ccgaaattgc aactgcaagg tctgaacctg tttctgtaat tttgaaacca ccacctacat
    39421 tcataaaaac atcataagtt gaaaaattaa agttaagttt tttttcaaga actgcaagaa
    39481 caagagaaat tctattataa tcaagtcctg ttactgctct tttgggaatt ccataataag
    39541 ttggagtgat aagtgattga atctctacaa taattgttct tttcccttcc tgagatggga
    39601 aaaaaacagc acctggaagt gtttttctgt cctgggataa aaaaattgtt gatgcatcag
    39661 gtatttcctc caatccatct tcttccattg aaaatacacc aatttcatca gttggaccaa
    39721 atctattttt tattgctctt aaaattctta aatttgtttt tgtctcgccc tcaaaataca
    39781 aaacagtatc aaccatatgt tcaagtaatt ttggtcctgc aattatccct tcttttgtaa
    39841 tatgtcctat tataaatgtt gatatatttt ttctttttgt gtaattgaaa agaaaatttg
    39901 cattttcttt aacctgagtt acagaaccag gagttgttgg aatatcaggg ttgtaaagtg
    39961 tctgaataga atcaattact aaaaaatctg gtttcatttt ttcaagagca tttttaacag
    40021 aatttatatc atttgaagaa agaatatata aattgtcaaa atttattttt attctctcac
    40081 atcttaattt tatttgattt tctgattctt ctccacttac ataaagaacc ttcatatttg
    40141 aatttgagat tattcctgaa attgtaagaa gtaaagttga ttttccaact cctggattac
    40201 ctgcaattaa aataaaactt cctggaacaa cacctccgcc taatacccta tcaaattcct
    40261 ctatttttgt ttttattctt ggttgttgct caattttaat ttctgttatc ttttttggtt
    40321 gtattattcc ttcctgttgt atttttgttt ttatattgac ttcctgaata aaagtattcc
    40381 aattgccaca gtcaggacat cttccaagcc atctaattga ttggtaccca cattcagaac
    40441 aaacatacat tgtcttttct ttcatttatc acctattagt atttaagaca caaagagtat
    40501 attgctgtgt ccactaaact ttttggtgga tataaaataa aattccccat acctcgttgt
    40561 gctatctggg agacgatgac gtaccaattc taaggcacaa ggtaagcaac tttagtcggt
    40621 tttacaaaga agcatagggg aatgtatttg gaacttaata cttttagttc ctttggaact
    40681 gagtatttgt attgcttcta tgtaaaatta tctaccccta aagaataatt tccagggatg
    40741 tgcttttata tctttaatta aaccaagaac ttcattatat aactcatcac ttttaacaaa
    40801 ttttccgata cttccctgtc cttcattcac atagttaaag agagaattta tgttttctgt
    40861 aatatctgta aaactttctg ccgattcttt cagttttaga gtcgtatcct gtaaatattc
    40921 catttctact tttatctgat ttaataggtt ctcttcgttc ataatttgag caataagtcc
    40981 tttattttgt aaattttcaa aaaatgtatt tatttcatct gctgtacttc ctattttgac
    41041 aactgtttct tctatatcct gagatgtttt tgcaaatttt cttatttctt tgcttgtttt
    41101 ttcaatttca tctttacttg taagaataag aaaattcgta ttattaatta gttcttctat
    41161 ttttggagaa ttttttgtta taatatccct tatttctaag tttgttttat ccactgatgt
    41221 ggaaaggtca tcaactttgc ttaaaactga attcatttgt ttaaccgcta aagaggtatc
    41281 acctattatt tctttaacat ttttttgaat ttctatatca gatgttaact ttcttaaatc
    41341 aactaaaaca ttatttaaat tcaaaattat atcctgccca attgttgcaa attgctctaa
    41401 tgatatagga tttactcctt caataatttc tccagcagct aaatattgtt gttctgacga
    41461 agggataatt tcaacatact tttctcctat tattcctaat gttctgattg ttataacact
    41521 tcctttcccc acttttacat ttctccttaa tgttaaagta accaaaactt ttggtctttc
    41581 ctttgcaaga atttgaatat ttttaacttc tccaaccatt actccactta atctaacagg
    41641 agaactaata tcaagtccac caatatagtc aaacataact ccaatttcat aagttctcca
    41701 ttgtcctttt tttgtttgac tgaaaataaa tataaaaatt aacaaaattg taattatcgc
    41761 aaacaaccca actttaaatt caagtgatag gtttctttta ttcattttta ccctccattt
    41821 caacaaaact tctaattaac gggtgattac ttttttctct aagaaaatca aaagttccaa
    41881 tttcaacaat tttcccattt tcaacaagtc caattctatc agatatttct cttgctaatt
    41941 taatatcatg tgtaactatt acatcagtag tattgaattt taaatgaatt tttttcataa
    42001 gagaagttat atttgaactt gttattgggt caagtcctgt tgttggttca tcataaaata
    42061 aaatttctgg tcttgaaatt aatgctcttg caattgagac cctttttttc attcctcctg
    42121 aaagttgttc aggcataagg tcctcaactc cttcaagtcc aacattttca agaacttcaa
    42181 tagccatttt tttaatttcc tctttgcttt tatctgtatg gtaaaggtaa taaaaaccaa
    42241 cattttccca tgtagtaaga gaatcaaaaa gtgctgaatt ttgaaaaacc ataccaattc
    42301 tacttcttat ttctcttatt ttttcttctg gcaaatttaa aatgttctgc ccattaataa
    42361 aaatttcccc ttccaccggt tctataagtc caattattgt ttttataagt actgttttcc
    42421 cacatccaga acttccaaca agaacaattg attctccctt ttcaacagaa aggtttaaca
    42481 tatcaagaac aactttcgct tcaaatacaa cttttaagtt cactactttt attatttcca
    42541 ttagacaaaa tagaagatgc ccgttaaaat taaattagta agaattatta ggaaaaacga
    42601 ttttacaact gcagatgttg aagcatctcc aactccttct gctcctcctt ttgctttaag
    42661 tccttcataa gaagaaacat ttattacaac aattgcaaat acaataactt ttataaaacc
    42721 aacaagaaaa tccttatatg aaataaacct gaaactttga taaagaaatt gtcctgatgg
    42781 aatttttata agaaaaacgc caattaaaaa tgccccacca attgcaagaa aaaaacttat
    42841 tacaaaaagt gcaggaagag aaaaaatccc tgctataact cttggactta ttaaatagga
    42901 aacagggtca atagcaagaa cttttaatgc atctatttgt tctgttattt tcataacccc
    42961 tatttcagca gcaattgaag cacctactct cccagcaaga gtgagagcaa ctaatacagg
    43021 tcccaattct cttgccattg ataaccctac aagtgcccct gaatacgttt ctgccccaaa
    43081 tgcttttaat gtatgcccaa tttccataac aagaatcatt cctgtaaata tagcaataac
    43141 acctacaagt ggtaatgaat taataccaat ttcctcaata tgttttaata tattttctct
    43201 ttttctctct gtaaatgcct ttttaagtga attccctata agaaggataa gagaaccaaa
    43261 ataataaaca ggagcaacaa tttttttacc aaaattttct ataaaatttt tcatttattc
    43321 tcttttggta taattttcaa atcttgtgta ttcttttatg aaagtaaggt tgacagtttc
    43381 ggtaggtcca tttctctgtt ttgctatgat aaggtcaaca attccaggtt tttcagagtc
    43441 ctctttttta taataatcat ctctgtaaag taataaaaca acatcagcat cctgttctat
    43501 tgagccactt tctctcaggt ctgaaagatg tggtttttta tcttctcttt gttctgttgc
    43561 cctgcttaat tgagaaatag caataacagg tatatttaat tctcttgcaa gtgattttaa
    43621 agatgcagaa atttcagtaa tttcctgctg tctgttttct gttcttctta cccctttcat
    43681 aagttgaaga tagtcaataa taataagttg tatattttct tttgctttta atcttctggc
    43741 tcttgctctt aactcaaaaa catttagaga aggagcatca tcaataaaaa ttggtgcttt
    43801 ttcaagtcgc cctgctgaaa gaagtaattt tgtaatttct ttgtcagaga gaattccatc
    43861 tcttaatttt ttataatcaa tccttgcttc acagcataac attctcccaa caagttgttc
    43921 tttgctcatt tcaagagaga aaaataaaat aggaaaatta tcaccacttg cagcaagatt
    43981 tagtgctata tttgttgcaa gggctgtttt tcccattgat ggtcttgaag caattataat
    44041 aagttctgat ggatgtagtc cacctatata cttatctaaa tcaaaatatc cagttgaaat
    44101 acctgtaaca aaaccctttt tattctggat attttcaata atatcaaggt tttcttttat
    44161 aaggtctttt aaaggatatg catatctatc aattttattt tggctgattt caaaaataag
    44221 ggattctgct ttatctaata acctttcaac atcctctact ccatttgatg cttctgttat
    44281 aatctgagta gcatttgaaa ttaaacttct taatatatat tggtctttta ctattttgat
    44341 atattcttct atatgagcag aagtaggaac aaattctgca atttctgtaa tatattcaac
    44401 tcctccaatt tcatctaagg ttccatcctg ttttaattga tttgtaagag taataatgtc
    44461 acacttctca tttctctcat agagtttaat tatagaagaa aaaatttgtt gatgagatga
    44521 actataaaaa aaatcctttt ttaatgtctc aaaaaccctt attcttgctt cttcatcaat
    44581 aagcatacaa cctaaaagtg ctttttctgc atcaatattt tgtggaggga ttttttcaat
    44641 atttaaatct ttttttttag cactcatatt aacctctcac tatccagacc ttaatttctc
    44701 cttcaacttc atctcctaat ttcacaggga ttttatatct tcctaacttt tttattggtt
    44761 catcaagaac tatctggtgt ttatctatat caattttaac ttgatttttt aattcttctg
    44821 aaataatttc agaagtaatt gctccaaaaa gtttatcatc ctgtcctgct tttgctgtta
    44881 ttgttaaaga aacattactt aacgcctttt ttgtatcaag agccatcttt tttctatttt
    44941 ctttttttct attttctatt atttcttttt ctttaatcat ttttatattt tggtctgttg
    45001 caggaatagc atatccagaa ggaattagaa aattccttgc atatccatcc tttacatttt
    45061 ttatttctct tgtccttcct ttacctgata attcttttat aaaaattact ttcatttttc
    45121 atccttttaa atttttacat atggcaaaag tccaataaat cttgcccttt ttatcgcttt
    45181 tgttaacatc ctttgatgtt ttgcacatgt ccctgttaat tttgatgata aaattttccc
    45241 tcttccactt ataaatcttc tgagtttccc tatatcctta taatcaattt cttttacttt
    45301 tcctttacaa aatgcacatt gtctattcct ggtttttacc atttatttca tcctcctttt
    45361 cttcaataat atctacattt ggtttttcaa gaaattgaaa attctcaaca actacttcaa
    45421 gaactgacct tttttcacta tcattatttt cccatgttct ataatttaac ctgccttctg
    45481 caaaaattaa atttcccttt tttaaaaatt cagcagcaac ttctgctctt ttaccccata
    45541 caactaaatt cacataacat gtttcttctt tcttttctcc tgtttttgca gaatattctc
    45601 tattaacagc aagtctacaa gttgttactg cctgtccaga agaagtgtat ctcaattcag
    45661 ggtctcttgt aaggcgtcct attaaaaata ctttgttaag attgggattc ggcatttata
    45721 atttcctcat gtggtaatat tttccttctt ataagtaaat atcttaaaat attttctctt
    45781 aattttagac tatcaattgt ctcttttatt gttccagggg cagtaaaata aaaaacaaaa
    45841 taaatacctt cagttctttt tttaattgga tatgcaagta tttttcttcc taaattttct
    45901 tttttgaaaa tctttgctcc tccatttaca agtacttttt caatatattc aatttctttt
    45961 tcaacttcat catctggcaa atcacttctt aaaacaaaac atacctcata tttcccttcc
    46021 attttctctc cttttattat atttttatat attataaatt attatgccta aaagtcaaca
    46081 ggaaaaaaat taaatttatt tcaaaactaa cttttttacc attacattaa aaaaacattt
    46141 gcaaacttat cttatttcat aattttatat ctacagaaat tctttttgaa atatagtaag
    46201 gagtaataga tatttcccct aattttgctt ctttctccct tccatcaacc ataacagaaa
    46261 ttacattttc ctcttttatc aggtcctcat aaatatagaa atcttcctgc cctccttttt
    46321 ctgaatatct cccaataaga acaccgttaa agtaaatcaa tgctttttca cttgtgtttt
    46381 tcaatgttat ccttaatggt gaaatatagt caggaatatt ttctattttt acttttttac
    46441 ggaaccatat aagagaacca gaagcccatt tttccttatt tatttccaca tcatcccaat
    46501 ctgaatcatc aaactctggg ctaaaataac ctcttgtctg tccttcaata ccctccctta
    46561 cctgccagtt tttatttaaa caaacatcat aagaagttat ttttactgtc ccattaagtc
    46621 cataatgagt tccccatcgg tctactttta ttccaatagt tatatagttt ttcccttttt
    46681 taactttatt tgtcaggtca aaactaaaag ggagagaaag aaaagcattt tctttaatac
    46741 cggaaaattt accattaaca aataaataaa tttttgcatt catcccactt tcaaaatcaa
    46801 tagaaattcc tcttttttca aagtcatctg gtatttctac ttctgttctg taccaggcaa
    46861 agcactcttc ataagaattt ttaaatagac ggctatcaaa ttttttatct acccttactt
    46921 tttcccagga actatcatca aattcatcat ttatttcttc catttcttct cttataattg
    46981 ccctttctgg atatgtatct ggaataaatt tccttctcca ccactttaat tctatacttt
    47041 tttcttctga agagagataa acaggagaat gaattccatt aaatgatttt tctccagaac
    47101 aaacatgtcc ttctgattca acaagaacaa ttaaagaatt gtctcctttt gataggtaag
    47161 atgatacttc acatcttaaa ttatctcttc cgctatttac atacttccca tttatataaa
    47221 tacttgcctc atcttgaaaa ccagttaaag tcaaatatag gggttttttg acttttgata
    47281 aattaaaatt acttttataa atactatacc catttttcaa aaaaccataa ttttccaagc
    47341 attccaatgg tctccacttc aaccatttat cactattatt ttctatattt tcttccttaa
    47401 ctttccattt tccagaaagt tcataatgaa tttctggaaa tttatcttct ggaagattaa
    47461 tttcagtaat attattttct tcataataac tatactctat attttcatta tcaagtttaa
    47521 cttctaatgg tttgaaagga attttcatcc agtttttacc ttcttttacc tgaaaaacaa
    47581 gatgaatatt attttctctt tctttttcct cttctaaaaa atagatatta gaaaaaattg
    47641 gatatttttt attcttatag ttaaaaaacc atgtttttga agatatctct ttatcaataa
    47701 taatcaattt aatgtctctt tcaactacta taaattgggg attattttta tgagtataat
    47761 taacattttt aatattttcc cctattttta gagatatttc tccgtcaata ccatcttctc
    47821 tataaagaat aagaattttt tctttaccat tatctatgaa atagaaaatt tcagaagttg
    47881 tataaagaag agttaaatca tcagaaagtt tcaaattcac aggaataatt gttgcactat
    47941 aaccaggtag ttgatattta ttttttgctg ggaaaatgat atttttacct gtctctgggt
    48001 ctttaaaatt tatcttgaat tctttattct ttccataggt attggataaa aacataaaaa
    48061 cacttttatt gtttttacgc ataaaaattt ctactccttt ttcatttact gaacattctc
    48121 cttctactgg aatggtttcc aacaatgcct cttgaaaaca ctctaagaaa ccacctattc
    48181 ttctaactat ataatatcgt ttatcaagtt ctccccactc tctaattggc gcttcataat
    48241 cataacttgt agatatatcc cttcctgtcc aatatccaat atttgttcca ccatgaaaca
    48301 tatatctatt aaaccctgat ataccagcag caatcagaca ttttaaatat acttcatcta
    48361 attctggtgg tttatatcca tcagaaaatg gcatctgtcc ataaaatctg ctataaaaat
    48421 gtgttcctac ttctattcct aatggcggtt tgtctggttg agttttaagt aaatttctaa
    48481 ttttattcat tacttcagaa attacccagg aaaaatgata tgggtcaggt ccttcaatta
    48541 tatccgttcc tcttatccag aaattttcat ttgtacaaat aggaatattt attccatctt
    48601 tttctactat ctcttttaac tttctatgat aaggaatatc tcctacttca aattcatttt
    48661 ctatttgata aagaaatatc tttcctccat tagtaattag atgtggtttt attattttat
    48721 tcactgtatt tagatatttt ttacaatatc caatgtaatt ttgctctaat gaccttaatt
    48781 cacaatcttt tggaatgaga taattaggca accctccaaa atcccattct gaacaaatat
    48841 atggaccggg acggacaaaa atataaagcc ccacttcttc tgctaaggat aaccatctat
    48901 taatgtctct atctttctca aatgaaaatt ctccttcttt tccctcatgc cagttccatg
    48961 caatataagt tcctatagta tttaaaaaag cccttttaca tttcaaaagt cggtctttcc
    49021 attgactttc tggaattcta aaataatgta tctccccact atttataaaa actcgtttcc
    49081 cattaaatat aaaacttttt ccatcataac ttatattttc atttttcatt ttttctccaa
    49141 aagtttttta taattgtttt taaaaataaa aaatttttac tttatattta taacatctta
    49201 tttaggaaaa gaaatttgta aatatttact caagtccgtt tatattctta ggcacaaagg
    49261 caaagagtaa tcaggaactg gtcttgcaat caaatagttc cacaggttcg cccgttgggt
    49321 cctcgctttt tgctatggat cttgcggacc agatcatgtc ctgatatgaa aatcaccccc
    49381 ctcatctatc ttctcccctc cgccacattc gcctatcatt gcgaatccga cgagcttttt
    49441 ggcggaccgc acggcggaca aagactgggg agaagaactt tttcctctta ttaccctcta
    49501 cctccgccac aatactcggc agacaggcac aggggagagg ctcgccgtag ggctacgccc
    49561 gaggagctgg agtgaggggg cttttgtgct tttaaattaa tgtggttgag taagtattta
    49621 cagaaattta ttcatcaaaa atgtatttca acaatatctt tgtcttccaa tatataatct
    49681 cttccaataa ctattggagt ttctgttttt cctctccaca gacgggcata tttgaaactt
    49741 ttaaccatgt ctttatgtat tatttctgca aggtgtataa gttgacttcc ttttttaatt
    49801 gtatatggat tttcaatatc aggtgattta ccaggtattt ttgtataaac ccttataatt
    49861 tctaaatgat taaacataat ttcaggtaat ttttctatat ttatcttttc ttttgctgat
    49921 atttcaaaaa aataaggaat tttatcttta tataattcaa caaaaacatc ctttatttct
    49981 ttatgagaag aaacatcaat tttgttgcca acccataaaa ttttataaaa aaactggaat
    50041 tccccctctt tttttacttt gaatttttct aaaacaccaa ttgcatcttc aatttcttct
    50101 attaaatcat cagatgaaag gtcaaataga aaaagtatta aatttgattt tcttataaaa
    50161 tcccctaccc aattatcggt aaaatccaaa gaaagagaag ggagttctat catctgtatt
    50221 tttatatcct gatattgaag cattccagga atagggacag ttgttgtgaa aggataatct
    50281 gctatttctg gttttgcctt tgtaagggtt gttaaaagag ttgattttcc tgaatttgta
    50341 ggaccacata ttactacctg agcatcccct tcttttggta tttgaggaac tattgatttt
    50401 ttagcagttg atgggctttt ttccatcatc tttttatatt ttgagatttt acttttgata
    50461 agtgcttgaa gtttttcaga tgatttatgt tttggcatta tagatagcat ttcttcaaga
    50521 actgcaattt tttcttctgg agttttaact tcttttaatt ccttttcttt ttcgtgatat
    50581 tggggtggta gattaacgac catttttatt tttcagaaac aatatttctt atgataggta
    50641 ataattttga tatattatat ggttttgtta cataaaaatc agcaccaatt tcataccctg
    50701 ttattctatc tgtattttct gactttgcag ttatcattat aacaggaact tttctactta
    50761 aaggtcctga tttaatcttt tttaatacat cccatccatc aattccaggt aataccacat
    50821 caagtaaaac aacatctggt ttaacttttt tgaataaagt taacccttct tcacctgttt
    50881 gtgctccata ggaaacataa ccagagtttt ttaatgcttg aagtaatacc tgcctttcaa
    50941 cggaatcatc ttcaataaca aggacttttt ttatttctcc catagtaaaa taataacaca
    51001 attagaaaaa tttttaaata cctcaaattg agacataata aatctacacg aaagggtatg
    51061 ttagacaaga caaaaaaatc tacttgaaaa ataggattta aatagcatct ccggataaaa
    51121 tagaaagaaa acggaggtgc aaatgaaaaa ggaattagaa atggggataa ggaagaaaat
    51181 aagcaaaaaa atagcagaga ggtatcagag agggcagaag aaagaaaaag ggaagatatt
    51241 agatgaattt gtaagtttaa tgggatataa caggaattat gcgagttggt tattgaggaa
    51301 tataggtagg aaggtggtaa taaaagggaa aagaggtaga gtaattttaa taggagaaca
    51361 acagaagata aaaagggaag gcagaaagaa agtatatggg aaagaagtgg aaaaagcatt
    51421 aaaagaagta tggtacatat tagattgtcc atgtggcagg aggttaggag attatatagg
    51481 agagatagta ccgatattag aggagaaagg agagataaag ataacagagg aagtaaggga
    51541 taaactttta aaaataagtg gaagaacaat agataggtta ttaaaagagg agaagaagaa
    51601 atgggagata aggggaaaga agaggacaaa gccaggtagt ttattaaaga gtcaaatacc
    51661 gataaggaca tttgcggaat gggatgaaaa agtacctggt tttatagaga tagatttagt
    51721 gagtcatgat ggagggaaag aagaagggat agttgtcaag tcaagttcgc catttaacaa
    51781 tttctgatat ctaataaatc cttattacga cttatttcct ccgatttttt tgatgttatt
    51841 gtagcgaaca aaaaaattta tagaagaaag aaaacaaaga gaagaaaaaa tatcttcaat
    51901 tgagag
//
LOCUS       DAOOVW010000005        51349 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_411_length_51349_cov_9.129196, whole genome shotgun sequence.
ACCESSION   DAOOVW010000005 DAOOVW010000000
VERSION     DAOOVW010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 51349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51349
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_411_length_51349_cov_9.129196"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..311
                     /locus_tag="PLW95_01215"
     CDS             108..311
                     /locus_tag="PLW95_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21289.1"
                     /translation="MVNNSILFREKKKIGIKIETFGDEALFTIEPISKDVKIDEIIKK
                     YKLITYKEYIKRATEVQDDARTK"
     gene            complement(304..1257)
                     /locus_tag="PLW95_01220"
     CDS             complement(304..1257)
                     /locus_tag="PLW95_01220"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOV21290.1"
                     /translation="MVKVCIIGAGGMANLVHYPSLSKIENAEIIGICDLNEERLRKTS
                     EKYKISNVYTDYKKMLEEKSPDAIYIIMPPHHLFDIVIHCLNKCLNVFIEKPPGITLE
                     QTKQMAKLAEKKGVLTMVGFQRRFAPLIVEGKKRIEEKGEVILTQANFFKNYLGQPPY
                     YNGAIDILTCDVIHSVDLLRYIGGDVKKVKSIVKGLFADYNNIFCSLIEFSSGAIGIL
                     SANWTSGKRVFSLEIHGKGIVVFVDPENQAMIYSDGKEEGESIDAKEFTGGKELFEIA
                     GFLDENKHFIECIEKKKLPETNFYDAVKTMELVENIYKNTI"
     gene            complement(1251..1814)
                     /gene="gmk"
                     /locus_tag="PLW95_01225"
     CDS             complement(1251..1814)
                     /gene="gmk"
                     /locus_tag="PLW95_01225"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013411812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOV21291.1"
                     /translation="MGGKIFVISAPSGTGKTTIQKQLVKLLPYLKTVITYTTRKPRIG
                     EKDGIDYNFVPIEKFKKMIEKDEFLEYANVYGNFYGTPKKEVIEKLKKDENVLLVIDT
                     QGGMNIKKIFPDAILIGILPPSIKEQEERLRKRGDLSEEEIKKRIKLAKYERKILMKY
                     YNYRVINKDIEETVEKIKKIIERSKKW"
     gene            complement(1815..2768)
                     /locus_tag="PLW95_01230"
     CDS             complement(1815..2768)
                     /locus_tag="PLW95_01230"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOV21292.1"
                     /translation="MRKNKINSKNWVFLFTAILLYIPLGFLQVSIDYNKKLENLESGL
                     LLMPGQVAGSLVLSGFKGLAADLLWLNIENYWHEGQHYKMLPLLESIAWLQPQYVTVW
                     SVGGWHMSYNIFAQVKINKENLIEEYKEKVKNLPEKDKKDLHNIIKIGEKLNFLIENL
                     GLKEGMEEDRITKLLTELGNYQYFLDNYKVNEENKFFIDALKEFIGIVDEMLYWYNQG
                     IEFLKKGVAYNPEKYDLYFELGWTYYHKGKDYKNAVRYLEKAIKFPHPDYVDDVLAHA
                     YEKNGQIDLAMKQWEKLLNTGFKSIAERAIQELETKGKFTP"
     gene            complement(2765..3529)
                     /locus_tag="PLW95_01235"
     CDS             complement(2765..3529)
                     /locus_tag="PLW95_01235"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOV21293.1"
                     /translation="MRKVWVIAVNTFKESLRKKTLYILVVFALIVIGTSKFFSFLTAE
                     EELKMIKDVSFSTIEFFGALIAIFGALGALSGEIEKRTIYTLLTKPVTRRNLFIGKFL
                     GMCLIILVNFILISLFFIGLLVFKKSPPDVETFKTLLLIYFELVLISTITLSVATFAS
                     DAFNIIFSFFLYIIGHLTSYGRQLVDRTESVILKGLGQMLYTIVPNYENFAIRDKVVL
                     GVPVSWSYVLKTLTYGILYTFIVILIGLYFFQKREV"
     gene            complement(3533..4288)
                     /locus_tag="PLW95_01240"
     CDS             complement(3533..4288)
                     /locus_tag="PLW95_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV21294.1"
                     /translation="MAIIETNDLTKIYVLGRGKEVVGLNKVSLKVEEGEIFGILGPNG
                     SGKTTCLKLLLGILFPTEGEISIMGKNQFDVEVKENIGFLPENPYYYDYLTGPELLYF
                     YGRLFNMNKEEIKERTEYLISLVKLSEVKKLTLRHYSKGMLERIGLAAALINDPKILI
                     LDEPTTGLDPIGCLETRDLLIQLKKQGKTILLSSHFLSEVERVCSRIGIFHRGYLLTT
                     GTLEGLISQYNANNLEELFVKTIEEFDSNYKKE"
     gene            complement(4350..5129)
                     /locus_tag="PLW95_01245"
     CDS             complement(4350..5129)
                     /locus_tag="PLW95_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOV21295.1"
                     /translation="MDKIKKLKKEIEYYLDKYLPSEKITPSILHKAMRYSIFSGGKRL
                     RPIIILLVGEILNIDKRKLMPFACGVEFIHNFSLIHDDLPAMDNDDYRRGKLTCHKKF
                     GEDIAILAGDSLIALGFQVIAGTKISSLISYVAKAIGSEGMAGGQVLDIISKDKNVSD
                     KFKRQRDEKKTGKLFEVCFLGPTFFKRVNDKEKKQLLKISKNFGIGFQIRDDIEDKEG
                     DIKKLKSKLSFIHKQLKKDIELFGKKGTSLLSIIDELFRLD"
     gene            complement(5129..6217)
                     /gene="alr"
                     /locus_tag="PLW95_01250"
     CDS             complement(5129..6217)
                     /gene="alr"
                     /locus_tag="PLW95_01250"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOV21296.1"
                     /translation="MDRWIEIDCGKIIENIKKIKNFTKRKFMATVKSDFYGMGAKYVS
                     KKIESVVDYFGVAIPSEGIELREIGIKKPILVLGPILPSDVEELIMRDIEITLCSDDV
                     FEQLKRVSKKRQKKAVVHIKIDTGMGRIGIQKEEAENFIKKVKEEKNIFTKGIYTHLA
                     TAEWENKEYAKYQIGNFYSVLEKVNELKNIPLKHIANSAAIINLPETYKNFDMIRIGL
                     LTLGIYPSKELAKKLKLQPALKGFCKVLFLKELPEGRYLSYGITYKTEKKTKVATLGI
                     GYGDGLRRFLSNKFELKWNNKKIKIIGNICMDQTLVDVSGKNVKIGDEIYIFGDNFEI
                     ENMAKIGNTVPQEILCGFGARRMQKVYK"
     gene            complement(6210..6878)
                     /gene="pyrF"
                     /locus_tag="PLW95_01255"
     CDS             complement(6210..6878)
                     /gene="pyrF"
                     /locus_tag="PLW95_01255"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOV21297.1"
                     /translation="MANLIISLDITEEEKMDFIIEKTENYVGWYKIGPVAFLKFGFCL
                     IDKLKKKNKKIFFDFKFFDIPNTVKNAVRNCCDIGIDMISLHILGGQEMIKEAIEERE
                     KTNKITKLIGITVLTSLTENDFGFSNIKKKVSILGENAFNWGLDGVVVGGEEISVLRK
                     YPYLLVVPGVRLKKENDDQQRIITPKQAVKEGADFIVVGRPVYDSKEPEDCVKKILQE
                     IKNG"
     gene            complement(6853..7941)
                     /locus_tag="PLW95_01260"
     CDS             complement(6853..7941)
                     /locus_tag="PLW95_01260"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOV21298.1"
                     /translation="MWNVVYLLGFLGSFLLSIFFTPFSGKIARKFNIIDIPSERKIHK
                     VPTPLLGGLGIFLSILATVLLGILFVRLNIIFLQLKIYIPGIIQSLPKLLLILSIGFI
                     VVLFGIFDDIFHLKPFVKLSLQIFVSIALFFSGIKISLFIPNNFISFIITTGWVIFMM
                     NSFNLLDNMDGLSAGVSLISGFILFIFAVQMGQLFVSTLLSVFLGAISGFLIYNFPPA
                     KIFMGESGSSFLGFFLATISIIITFYKYETTNPYLPIFAPLVIFSVPFFDTISIIWIR
                     KKRKLPIFKADKNHISHRLVNLGMNKKQAVIFIYFLTICTGLGALLMKNLNIWGCLII
                     IIQVIIILSIVGILELVGRKNGKFNNIS"
     gene            8128..9330
                     /gene="argJ"
                     /locus_tag="PLW95_01265"
     CDS             8128..9330
                     /gene="argJ"
                     /locus_tag="PLW95_01265"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HOV21299.1"
                     /translation="MRNNKRGGITYADGFLSSGIYSGIKKEGKDLSLIYSKLPCVAVG
                     TFTTNKFKSYSLIVTMKNIKNPIHSIIVNSGNANACNGKENYIKTKEVVGKLAEKLKV
                     KEENILFGSTGIIGAPLPYQKIISSLDELINSLSCSGHTAAAEGIITTDTFLKEFQIN
                     TGIKGRKKEIFIGGMAKGAGMINPDMATMLSFITTDAVIERDALETALKQAVDKSFNM
                     LVVDNDTSTNDMVICLANGAARNKRIHYNTDEYFKFYQSLENVCINLAKMIAKDGEGA
                     TKFIEVKVKGGWCEKDVKKVAKKVCGSNLVKTAIYGCQPNWGRIIASVGSCKVKFNPE
                     KLELKICGINIFQNGNVINFDNNLLRQKLQDKEILIEIDLKQGKYQTSSFGCDMTEKY
                     IKINKEYE"
     gene            9327..11267
                     /locus_tag="PLW95_01270"
     CDS             9327..11267
                     /locus_tag="PLW95_01270"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOV21300.1"
                     /translation="MIAKMLKFHLFFLGDENKLLRTLQKKGIVEINQLEKEYGFENLK
                     ERVISIEEKINKIEFLKGIVKKIDGKEFSGKILLTESQEQKIISNFLVDETFDNFKKL
                     NDEIERRKIVDRKIETIINQLNKFKNIKLNFATLFSLKNFSFYIFTSKKPISLISEDI
                     LFEKVYQDKKEIFYLIVFPSGKKEEIFEKIKENKGEIIEVKKWNKYPEDIIKKLLKTK
                     KKNLEKINNFEKYIGENIKKIKNEIFVIYDHYISLLDYLKTKEKVTSSKFIKCFCGWI
                     KEKDIEKLKEAIKETSPDSFLHIVPPQKDEDVPIVLENPPLIEPFEVVTDLYGRPIYK
                     NMDPTFALSLFFVISFGFCTSDAGYGILLILLSLILMRKFKFYPSFIKFLKLLIYGGI
                     GAIIIGALTGGWFGDIISKLPEKSIITKTLSSFIILDPLKEGNNALIFLAWALIIGYI
                     QIIWGLILNLYNSIKNKVITDILLATSTLSVQIFAGVLVFLFLKGLKTGIFFYIPIFI
                     VILNFLTIMAVNATSQKGLLIKGFWAIYSAYSVIAGNLLGDVLSYSRLFGLGLTSTVL
                     AIVVNQIVFMAGGIPFIGPLFAVIIFLLGHFGNLAINLLGSYVHTSRLQYLEFFTKFY
                     QGGGKPFSPFSEKRIYTYLKNF"
     gene            11567..12238
                     /locus_tag="PLW95_01275"
     CDS             11567..12238
                     /locus_tag="PLW95_01275"
                     /inference="COORDINATES: protein motif:HMM:NF017373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21301.1"
                     /translation="MGKESIWSPTVALAQKFWLGLSKVIAAVLIFLIGWLIARLLKNL
                     IIRFFKVIKIDKLGEDSGLKGLLEKGNVTRDISELIGIGVYWLLMLVVIFIAVDFAGI
                     LIPEEVINTLLGFIPKFILGLLIFIFSVFLGNFFNGIVRTSAGNMGIEKASLLGKVTH
                     ISIVAFGAVIALQQIGIAPTFIANVFIIVLGSFCFGIALAFALGCKDIIKNYLEDHFK
                     KDKKE"
     gene            12245..12316
                     /locus_tag="PLW95_01280"
     tRNA            12245..12316
                     /locus_tag="PLW95_01280"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12277..12279,aa:Ile,seq:gat)
     gene            12332..13021
                     /locus_tag="PLW95_01285"
     CDS             12332..13021
                     /locus_tag="PLW95_01285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOV21302.1"
                     /translation="MEKIEFSAEIREGTGKKITRKLRKNEYIPAVLYGAKEPPLLLKL
                     KRKDTEKTIRHIESHSIMADLIIDEKSGKKENVKVVLKEIQVNPIKENITHLDFYRVS
                     MDKPIFITVPLHFIGTAPGTEKGGILDEEMREITIECLPKDAPSFLEIDISSLDIGNT
                     LLVKDIKTDKKIRIVENGERVVISVLAPKVVEVEEEAVEKEEVKKEPEVITEEKVEER
                     RKEKEKEKEEK"
     gene            13018..13578
                     /gene="pth"
                     /locus_tag="PLW95_01290"
     CDS             13018..13578
                     /gene="pth"
                     /locus_tag="PLW95_01290"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOV21303.1"
                     /translation="MKIFFGIGNPGNEYKNTRHNAGLMVIENFLDKFENYTEKKHRYF
                     ISYEISLGGKETILVKPRTYINESGISFKKVLDTYKVDVENVIVIHDDLGIETGKIKI
                     VFDKGDNGHKGIISIFNTVETKNFYRIRIGIGKGKIEITYVDYVLSPFEEEEKELIKS
                     SIEKAVGAIEEIIRSGIERAMTIYNR"
     gene            13593..15680
                     /locus_tag="PLW95_01295"
     CDS             13593..15680
                     /locus_tag="PLW95_01295"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOV21304.1"
                     /translation="MDWSQVITIIGSVFALGYTVFIVKGILKEKEGTPEMIEVAEAVR
                     TGARAYLKRQYKGVSMFFGIVFIILLLLAFLKYLPIFVPFAFLTGGFFSALSGYIGMT
                     VATKSSSRTTFAAKTSLNKALRIAFSAGFVMGLVVVGFALFDISIWYNILNFVYRHLP
                     LEEKLKNITSTMICFGMGASSQALFARVGGGIYTKGADVGADLVGKVEAGIPEDDPRN
                     PAVIADNVGDNVGDIAGMGADLYESYTDSIIATMALAYAAGLSIKGIAVPMVIATVGV
                     ICSIIGSLFVKVGEEASQANLLKALRRGTYLASILTFIISYFVIIKILGRGYIGLYGS
                     VISGIFAGVIIGFFTEYFTSDNYSPTRNVAIAAKTGHATVIIEGLATGMFSTIAPVLT
                     VVVATLCAYILPGGFADPALGLYGIGLAGVGMLSTLGITLATDSYGPVADNAGGNAEM
                     THQPSFVRQRTDSLDALGNTTAATGKGFAIGSAALTALALIAAYKDQVVLLGGKIDFS
                     IMNVRLLLGIFIGAMYPFFFSSMALRAVGRAAQKIVEEVRRQFREIKGLIEGKAKPDY
                     AKAVDICTKAAQKEMVIPTVFTVILPIIVGILLGTEGVIGLLVGAVTSGFAVAIMMAN
                     SGASWDNAKKYIEKGNLGGKGTEVHKAAVTGDTVGDPFKDTAGPSLNILIKLISIISI
                     VFASFIVKNSLFK"
     gene            15902..17059
                     /locus_tag="PLW95_01300"
     CDS             15902..17059
                     /locus_tag="PLW95_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004404901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HOV21305.1"
                     /translation="MKNIFEKSIQLHKKAKGKIEIKSKVPLLDKNDLSLAYTPGVAGP
                     CEEIKKDISKIYKYTSKWNLVAIVTDGSAVLGLGNIGADASLPVMEGKAILFKEFGDV
                     DAFPICIKSQKIEDIVKTISLISPVFGGINLEDISSPRCFSIEKKLEKILDIPVFHDD
                     QHGTAVVVLAGLINALKLTNKKFNKIKVVINGAGAAGIAITKMLYKTGVREIFLCDRV
                     GVIYKGRKENMNSAKEEITKYLSKKSKKGTLNEVIEGSDVFIGVSGPDILNEEMVKKM
                     NKNSIIFAMANPIPEIMPDKAKKAGAFIVGTGRSDFPNQINNLLAFPGIFRGALDIRA
                     KKITDEMKLAASYAIANYIPENKLSPSYIIPSPLDKKVAKKVSEKVSSAYL"
     gene            complement(17054..18415)
                     /locus_tag="PLW95_01305"
     CDS             complement(17054..18415)
                     /locus_tag="PLW95_01305"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOV21306.1"
                     /translation="MKVGFGKVVITPPVGSSLQGYFQDRRSVDIRDDLYASCVVFDDN
                     DDIFVVISCDLCNLSDNITKKVREILLKENGIKKGNVLIHATHTHTGPVVDFTEESIY
                     TKGFYIEKSYLEVLPFYIASSVKIALRRRQEAKIAVGKNEVDGISFNRRYLMKDGRVI
                     TNPFNQIENIVKPAGEVDKTLTFLKIDDNESGKIKGIIVNFALHPDTLGDNLISSDWP
                     GFLRDELKKEFGEIEVIVLNGPSGDINHINPFDYKTRSTNIGKEIAKKISEKVKEKID
                     VKATEIKKIQPFYFPMNLDYREITEKVYRKAKEILKKEKKTFLKYMIASGIVSIYKEK
                     RKNKSIKVDLNGVKIGDKTILIGICGEIFVEIGMNIKKILGIENTIIAQNSNWHLGYI
                     PTKRAFQQYEKNLEIKEIEDIDLTEAIGINTSYETLPMASKVNKNTEEKIYSTISKIK
                     AIS"
     gene            complement(18434..19345)
                     /locus_tag="PLW95_01310"
     CDS             complement(18434..19345)
                     /locus_tag="PLW95_01310"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOV21307.1"
                     /translation="MEEKEIRIGIIGFDTSHVTAFTKLLSDKNDPYYVKGGKVVCGYP
                     SFSPDLEASYSRVDGFKKELVEKWGVEIVNSIEELLDKVDAVLLESVDGRRHLKEAKP
                     VIEARKPLFIDKPMAANYKDAEEIFKYANKFNCPVFSSSSLRYDYNIQNVKKQVGNVF
                     GCDAFSPASLDPTNPGLFWYGIHGVEILYTFMGIGCKQVICEVTNDFHFVVGKWNDGR
                     IGSMRGLRKGAYEYGATVLEEKNVYQVIYSKEVPLYSQLLKEIMKFFQTGKSPVAEEE
                     TLEIMKFIQASLVSEKENRKVNLSEIK"
     gene            complement(19326..20399)
                     /gene="ispG"
                     /locus_tag="PLW95_01315"
                     /gene_synonym="gcpE"
     CDS             complement(19326..20399)
                     /gene="ispG"
                     /locus_tag="PLW95_01315"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004440830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOV21308.1"
                     /translation="MIRKNTRKIKIGNIYVGGDSPVIIQGMTKVPTSNISFLLKESKK
                     LENEGAEIIRISILNEKDTDSIKVLKKEIKVPIVADIHYNKKLALYSIEKGIDKIRIN
                     PGNMKRNDLKEIIKQAKNKNIPIRIGINSGSIKVKRSLVENVIEKTTETTKFFQDNDF
                     NDIIISAKTPFVKETIEIYRKLAEKFNYPLHLGITEAGKGYMAESKSILGIGILLNEG
                     IGDTIRVSLTESSVKEIKIAQSILQALGLRRFEPEIISCPTCGRIQVALFDVLKKVEK
                     EILRLNKKYPFVKNLKIAVMGCSVNGPGEAKQADIGIAGGVGKFAFFKKGIIIGTYKK
                     EEIINKLIKEIIKMEEKNGRKRN"
     gene            complement(20396..21475)
                     /gene="rseP"
                     /locus_tag="PLW95_01320"
     CDS             complement(20396..21475)
                     /gene="rseP"
                     /locus_tag="PLW95_01320"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOV21309.1"
                     /translation="MIISIIAVIFLFSVIIFVHEGGHFLFAKSAGVKIETFSLGFGPK
                     LFSWKRGETLYQICLIPFGGYVKMAGEEYLDKGEFEKWEYMGKPPGVRSRILVGGSLN
                     NLILGFLLLIPSFMLGLPGYDGTKIGGFVENMPAEKVGLKVGDEIVEINGKKCKEWFD
                     VLTVIKKEAKKNPEKEIELKVKRDNKILQFKIKPVSYTEDTLTGEKEKVYVIGILPKE
                     IEEKYPFQQAVIRAGKEFYKMSYSVFLAFKMLIKKEVSTKLLSGPVGIAQWSAEIVHY
                     GISKYIYFIAFISINLGIINLFPIPLFDGGHLVGLFFEKITGFRAKRRFLEIVQYVGV
                     ILLITLALYVTYNDILRVISSLIKK"
     gene            complement(21503..21838)
                     /locus_tag="PLW95_01325"
     CDS             complement(21503..21838)
                     /locus_tag="PLW95_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21310.1"
                     /translation="MEQQNIQEGKSIAWLSYLGILILIPVLIQKDNPYTKFHIKQGLI
                     LLIISIGWWFIHFIFMFIPFLRTIFYFLSYLVYMFVFVLMIIGIVNSLQGKTDCLPLI
                     GKYGEKINF"
     gene            22045..22965
                     /gene="trxB"
                     /locus_tag="PLW95_01330"
     CDS             22045..22965
                     /gene="trxB"
                     /locus_tag="PLW95_01330"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOV21311.1"
                     /translation="MEKEIYIIGAGPAGITAGIYALRSKIKTTIIEKFSPGGNMLITE
                     TIENYPGFPDGINGLELAERMKKQFLNLGGEIITKEVVDIDFKKDRKTIFLSDGKFID
                     TDVFIIATGSSRKKLGVKGEDVFIGKGVSYCAVCDGFFFKNKNIAVIGGGDSALKEAI
                     YLTRFVNRCYLVHRREQFRGSKYLQELIENNEKISPIVPFVVEEIIGKEKVEYLLLRN
                     TKTDEKMELKVDGIFISVGQKPNVEFLKGKLKSNPSGYLIVNEKMETSTKGVFACGDV
                     IKKDLYQIITACGEGAIAAFFAEKYLDSLD"
     gene            complement(22962..23525)
                     /locus_tag="PLW95_01335"
     CDS             complement(22962..23525)
                     /locus_tag="PLW95_01335"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOV21312.1"
                     /translation="MVLLIDNYDSFVYNLYQYIGELGEKIVVYRNDKIKIEEIKKIAP
                     SRIIISPGPGRPENAGVSCEIIEKFAGQIPILGVCLGHQCIGYVFGGKIIGAQKLMHG
                     KTSQIYHKGKNIYKNLKNPFQATRYHSLIIEKKSIPSVLEIDAWSEDEEIMGIRHKNY
                     KIYGVQFHPESILTEEGKKLIKNFLDI"
     gene            complement(23525..25009)
                     /gene="trpE"
                     /locus_tag="PLW95_01340"
     CDS             complement(23525..25009)
                     /gene="trpE"
                     /locus_tag="PLW95_01340"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012459918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HOV21313.1"
                     /translation="MKIYPDYNVFSYLSKKYNIIPICREIIGDIETPVSAFMKIKSGV
                     YNFLLESAEQEERTGRYSFIGTGPELIIKGKNNEIEIYDNTTGESKKLYNNQPLKIID
                     EYLKKFKTPILPQLPPFTGGFIGFISYDFIRTIEKLPSLSYDDLKVPDFIFILSKDII
                     VFDHFKRRILLITNLSIEKENKSLKSLYDEGIEKLEKMKVKLEKPLYMVEPLKKKQIT
                     KEFKSNFSQKQFLESVKKIKKYIKNGDIIQCVPSQRWEKKLDTQPLNIYRSLRMINPS
                     TYMFFLQMEDTNLIGSSPEILVRQEGTKAILRPIAGTRKRGKDEKEDKKLENQLINDE
                     KEKAEHIMLVDLGRNDLGKVCIPGSVKVTDFMKIEKYSHVMHLVSNIEGIIKKGKSPI
                     DLLISTFPAGTVTGAPKIRAMEIIEEIEPTRRGPYAGAVGYFSFQKNMDFCITIRTIV
                     ISKEKAYIQAGAGIVADSIPTREYYETINKSKGPMEAYEISGEI"
     gene            complement(25059..25247)
                     /locus_tag="PLW95_01345"
     CDS             complement(25059..25247)
                     /locus_tag="PLW95_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015390247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOV21314.1"
                     /translation="MRVKIDESLCTGCGLCVDICPEIFEMEDDVSNVKIKEISSDLEE
                     KVKEAAESCPVEAIIIEE"
     gene            25352..25831
                     /locus_tag="PLW95_01350"
     CDS             25352..25831
                     /locus_tag="PLW95_01350"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOV21315.1"
                     /translation="MSKVVIIYSSKTGNTKKMAESIYDGVKEIIQNVELKSITDIKVD
                     DLLTADGIIVGSPTYFGLVCSEIKKFFDESVVHFGKLEGKVGGAFASSANIAGGNETT
                     ILGLLQMMLVHGMVIQGNISSGHYGPVSVGFPDKNVLNECRAYGKKIGKLVKKLFDK"
     gene            25851..28595
                     /gene="ileS"
                     /locus_tag="PLW95_01355"
     CDS             25851..28595
                     /gene="ileS"
                     /locus_tag="PLW95_01355"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOV21316.1"
                     /translation="MDYSKTVYLPRTDFPMKANLSEREPHFLKIWEEKEIYKKIIKKN
                     ENGEKYILHDGPPYANGHIHLGTALNKILKDIVIKYKSLKGYYSPYKPGWDCHGMPIE
                     HQIFTQLKLKKNQVDKIDFRKKAGVFAKQFVNIQREEFKRLGVLGEWEEPYLTLDKEY
                     EGDIIKTFGQLSLSGYIYQTYKPIFWCINCETALAEAEIEYYDIKSPSIYVKFKVIQD
                     NKNNFRDNTYFLIWTTTPWTLPSNTGIALSPTLNYAIVQTEKEDLIIGEKFVSQLLEK
                     KGIKGNIKKVVKGTDLEGVLYEGPIIKRKSKVIMASFVSDQEGTGCVHIAPGHGEEDY
                     NAGIKNGLEILSPVDEKGTFTDEVKEFAGKVVFDADKLIIAKLQKEGNLFLEEEIVHS
                     YPHCWRCKKPVIYRSTKQWFLKVDHKNLRELLKKEIEKVNWFPPEGKNRIYSMVELRP
                     DWCLSRQRIWGVPIPVFYCKNCKKPIITEETIKEIEQQIRENGSDIWFEKEEKQLLPE
                     NFLCPYCGGNEFEKEKDILDVWFDSGVSNISVLKEKYGLKWPADLYLEGSDQHRGWFQ
                     TSLIVSCGTKGKAPYKDVVTHGFVVDAEGKKMSKSIGNVITPDQIIKKYGAEILRLWA
                     VSENYQKDIRISEEIIKNLSKTYRNIRNTIRFLLGNLYDFEIEEKIEYDNLFEVDRWA
                     INKLKEVVRKVDNYYNSFNFNKGFEEINNFCNISLSSFYLDYLKDRLYTYGKNSKERK
                     TAQQVLLEILSSLLMIISPVLSFTSEEAYQFLKWKDKESIFLNTWPDLGEPDKKLLDR
                     WEQFFQVRKDILKKIEEKREEKLIGSSLEVKVTIKTEKEQIEFLKSFQNIEKLLMVSQ
                     VNFIEGKEEIIVEKTDYKKCQRCWGYFPEVGRNTEFPDLCDKCIKVIKENVNRLESSE
                     "
     gene            28826..29305
                     /locus_tag="PLW95_01360"
     CDS             28826..29305
                     /locus_tag="PLW95_01360"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOV21317.1"
                     /translation="MKYLIDGYNVIKSSYLGKEGDLKGKLSLYQILDNYKANHLSVIF
                     SVVLDGFNTSDFPFLRDKRIKTFFSGEITADEYIKKILEKGKRNEEITVVSDDKQVQI
                     SSKLFGCNVMSVDTFLNTVNPKGNNKPKGPIEKKISYSKKKKIEEELKNYYELRKNT"
     gene            29283..29903
                     /locus_tag="PLW95_01365"
     CDS             29283..29903
                     /locus_tag="PLW95_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21318.1"
                     /translation="MNYEKILNLKKLENEFRDFFEQQDNVREETLKLVREIGRSAFKS
                     VKEIHQGKIKEAEEEIENGLKILKKAKKLLSNFPEIYYAGFLHSAEKEIIEALITLYL
                     IRDKKIPEFNKDDFDVKSFLHGLCESTGEIRRYILDKIRSGEIEEIELYLEIIDEIYY
                     FVLEFQYTDALTRGLRRQIDYMRGIVERTRADVTSAIISFRKDEKK"
     gene            complement(29958..30227)
                     /locus_tag="PLW95_01370"
     CDS             complement(29958..30227)
                     /locus_tag="PLW95_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOV21319.1"
                     /translation="MNKGQLVDEVAKVVCSKKEAVAAVDAVIEAIKKALKKGDVVTLV
                     GFGTFKVAKRKARTGRNPQTGATIKIPAKKVPVFRPGSELKEAVK"
     gene            complement(30411..30482)
                     /locus_tag="PLW95_01375"
     tRNA            complement(30411..30482)
                     /locus_tag="PLW95_01375"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30448..30450),aa:Cys,seq:gca)
     gene            30597..32126
                     /gene="dnaX"
                     /locus_tag="PLW95_01380"
     CDS             30597..32126
                     /gene="dnaX"
                     /locus_tag="PLW95_01380"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOV21320.1"
                     /translation="MYKVLARKYRPLNFDEFVGQENIVETLKKAIEKNKTAYAYLFAG
                     PRGSGKTSMARVFAKALNCQNGPTINPCNKCEICNEISLGTSLDVIEIDGASNRGIDE
                     IRTLRENVNLRPSKARYKIYVIDEVHMLTTEAFNALLKTLEEPPEYVKFIFATTAPEK
                     IPPTIISRCQRFDFKPLTLKEIENKVLEISKKENFTIEKKAIEEISEFANGSLRDVLS
                     IIDQLIVFSENNIKIEDVRKLLGIVDEKGIDEILKLLVEKKVKDAIVFLHNLISLGKD
                     PSLIMEGIIKRFRDILFYKIEGGEKKEIFSSFDNITTDELLQASSIVIEFKEKLRRET
                     QPILLSEILLFKLSSILGKEIKNNENQQVDIFEITKKEKNIKEETKEEIKKDTKEMII
                     EKESRENLIDDIIGNWDIILKEIKNKSRILEAAIREGKVEKFENDTVFISYEKKFNFH
                     KKRADENKKEIEKIISKLTGKNIKIETLLKNDTKKNIIEDEDVKKILEEFNGQIIEKE
                     E"
     gene            32130..32426
                     /locus_tag="PLW95_01385"
     CDS             32130..32426
                     /locus_tag="PLW95_01385"
                     /inference="COORDINATES: protein motif:HMM:NF014616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOV21321.1"
                     /translation="MFDNLKQLAQLKQQANKIEQLMRSKIFESSSPNDEVKIKVNGKM
                     ELISIEIKEDILKKENKAYIEKIIKKTFSQCQKEVEKWMATEVGSQLGSFKLPF"
     gene            32441..34735
                     /locus_tag="PLW95_01390"
     CDS             32441..34735
                     /locus_tag="PLW95_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21322.1"
                     /translation="MVKIENGLISFCLNEILFKECLMSLDVDDGILKSVSSEISIKDK
                     SNFILHCKFPEKNIEWEIFFKESNGEQVIESKIMNYGKRAVKLGKVFLLDTKIEGFRN
                     EQDEVAVFPLQIYGQKVYRIDDPEMPENSQVIVQFCNNTKNLSLQGAFLTFQRCNTKL
                     VVKRSVHGLDGLQAYCDFAGWELAPGKSTSTEIFRIIVGDNPYTQLENWADKVKDIIK
                     PDIWENTPLGFNGCAWAIFDGKDNYAKVVSENLNAINKRLAGFGFKYLWTSMHNFEGG
                     LPGNWLKWNYKHIPCGRKKFIKMVRSKGFLPGFWIAPFYLCSSLTDLLKELEDAILQN
                     LEGEKMIVCEEWRHGDAGKLPKKERPCIYALDPSHPKTIKFLKNVFKTYRKWGIRYYM
                     VDFLEAGAGNIHRFPYKKHFNKKLVAGPEVYTNALKILKKTAGKDTYLLSSTGPQLHN
                     AGILDGVRVGNDFGEGRDINKESFFYPASYVINKCDFWTGPKEALLSQAANYFTHRKF
                     YIADSSNLLTVDKPIPLTHAQIHATIHAFSGGPTILGDDIRNISEERLNIIKKTLPRS
                     KEIGFPVDLFTSVSPDYPKLFLRSIVKPWGEYKVLAIYNFDPVPKNIKVPFETLGMEK
                     GQSYLVWDFWNEKFEGKRIDNIDINVRQESVTVLRFVKDKEQPVILGTDMHIMMGEME
                     IKDFEYNSKKMECCFTVERPKGESGTVFIYAPENIYVKKTDGLHISKDGNYNTLVIAV
                     PLKFKKKVVKKIINFGQMEKDCNC"
     gene            34760..35353
                     /gene="recR"
                     /locus_tag="PLW95_01395"
     CDS             34760..35353
                     /gene="recR"
                     /locus_tag="PLW95_01395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOV21323.1"
                     /translation="MKYYPEIVEKLIENFSKLPGIGPKSAERIANFLITGDNEFIDNF
                     VENLANLKKKIKICQNCFNLSEDNICNVCKDTTRENIICVVENIKDLVIFEKASFKGK
                     YHVLWGRISFLDKTDPDDLRIPQLIERVQKEKTKEVIIATSTTKEGEDTAAYISEILK
                     KLNIPHSRIGYGLPVGAEIEYVDLQTIKKSIEGRRGL"
     gene            35353..36234
                     /locus_tag="PLW95_01400"
     CDS             35353..36234
                     /locus_tag="PLW95_01400"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOV21324.1"
                     /translation="MFCLIYKFCCFLGIHLPLKVAYFIAEQIARLRYIFFPSLRKTVR
                     NNIKKTLAYRKKKFGIDYKSYDVIKITKKAYLNFAKYMTDFFNIPKWDIEVVKNKVFL
                     ENFNLLDKCLEKGKGVIALTAHIGNWELAGIVASILGYKVSAIAIPYLSPSVTKIYVE
                     RRKDKGVDVILTGSNPKHIIKALRENRVLAILGDRPFTEKGIYVKFMGENVLFPRGPA
                     TLFIKTKAEYIAGFLIMEGKNYRMFFREIEKPPSNLTEEEKIKFLTQKGANIIEELIL
                     AYPSQWLNFSNVWDNSA"
     gene            36295..38022
                     /gene="dnaG"
                     /locus_tag="PLW95_01405"
     CDS             36295..38022
                     /gene="dnaG"
                     /locus_tag="PLW95_01405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOV21325.1"
                     /translation="MKGYISQEEIVRVSEKVDIVEIISRYVNLKKSGKNYRGICPFHT
                     EKTPSFFVNPEKQIFHCFGCGIGGDVFKFIMNIENISFPESVVKIAKEVGIEINFQTK
                     QISNKDKQEIVKANEFALKIYSEVLFSSQGKQASEYLYNRNFDDIAIKNFSLGYAPYD
                     RNFLLKKINEHSLAKDVFIKSGLISENGEKDVFTNRIIFPIINTNNDIVGFGGRTIEE
                     NGLPKYLNTKENIIFNKSRTLYGINWAKETIKENNYAIIVEGYFDVLKLLSNNIKNVV
                     APMGTSLTEGHLNILKRFTDKILLVFDSDTAGINASIRGLENILKSGFEVKLCPLPTG
                     FDPDKFIDEFGTSSFIKLLNNSQNFIDFMITNESKNFDINSPRGKSIIIKEISKLLSV
                     IPDEIEKEEYIKYLSSKTSIPQETIEKYVEENSQSKEEIKPVQKKPLKPNAEIYLLDI
                     ILNDQTYLEQIIEKKEKLTEKLKKVVEAVEILINKNIKPTVANIIGIIDDEQILNLIS
                     NVAIEDNLISEDKKRKIFDDCLKKVEEISLKETLNKRKKEIFEKGPSPSKNELEEIQT
                     IIYQLQKGR"
     gene            38024..39199
                     /locus_tag="PLW95_01410"
     CDS             38024..39199
                     /locus_tag="PLW95_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOV21326.1"
                     /translation="MGRNKKNLSKGEQKKKISEIIELGRKKKHLSLEEINDILPIDIT
                     DQSEIDNLFDELDHWDIDIDEKEPFSIEKEKIIDTEEAKNALRSYMKIAGSYSLLTHE
                     KEVELAKGIEEMKKKINQMKKRKKISQVQYMNCCQQLNELRGLLIKSNLRLVINIAKR
                     YNNPKLSILDLIQEGNIGLMKAVEKFKYKTGFKFSTYATWWIRQAITRAIADHSSTIR
                     IPVHMIEKINKLKKLEAKSNQGNNGELEDEEIAKKLKIPVGKIKNIRRSMRPDPISID
                     LPVGDEERATIGDFIEDNKSPNPLKRVKQSLLAEEIEKALKVLDEREEIIIRLRFGLD
                     EKRYSRTLEEVGDYFNLTRERIRQIESKAIQKLKNSIHSKKLALFLEGSFETSIPTK"
     gene            39220..39981
                     /locus_tag="PLW95_01415"
     CDS             39220..39981
                     /locus_tag="PLW95_01415"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOV21327.1"
                     /translation="MSFIKFLGTAGARFVMLKQLRASGGMWFSINNTNFIVDPGPCCL
                     FRIIKSKPKLHPEKLDGIYLSHKHLDHSCEINLMIEAMTEGGREKQGIVFTPLDAISN
                     DPVILKYIRNFPEKIEILQEGKEYNLKDIKFEIPVRHKHGVETYGFKLKSANIPVISY
                     IPDTAFFPEIIEKYKGSDILIINTVLYEKKENVLHLSLQEAEDIISQIKPQKAIITHF
                     GMTMIKNKIWEKQGEISKRAKTDVIIAYDGMNLEI"
     gene            40016..40996
                     /locus_tag="PLW95_01420"
     CDS             40016..40996
                     /locus_tag="PLW95_01420"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOV21328.1"
                     /translation="MFLLKNIFSGKKEFYFRLSSLLSSGFDIISAISNLEKNNPLHQK
                     EIVSFIKREISKGKNLSEAMKNFSLFSNFEIKSISAGIKSGNLPETLLSLSDYFSFLE
                     NIRRNIISGLTYPFILLNLAIIIPAIPTLFLKGFFPFLGKIIIPFIIIYGSIFVFGYL
                     PDIYKKRNLREKLDEIIIKIPFLGKILVNFQVVIFLKTFVILYKAGIDIISSFRDATE
                     VIGNETIKKQFDKNFALLKENIPVSEAIKNNQFLPDEVKGMIETGEISGNMDKTIEKV
                     IQYTEEETKFIINQILKVIPVVIYLIVAAYVGYIIISFYSGYIKTINSFL"
     gene            41878..42639
                     /gene="cas6"
                     /locus_tag="PLW95_01425"
     CDS             41878..42639
                     /gene="cas6"
                     /locus_tag="PLW95_01425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HOV21329.1"
                     /translation="MRLNITFFSEKEIILPISYNYILQSFIYRNLNTFLSNFLHKDGY
                     IYEKRSFKLFTFSRINGKFRKEQENFIFSPPISFILSSPKEEILECFAETLVKKNIFI
                     LNNNFIYIEGVEVQFYPNFSKKEYEIKMLSPVTVYSTLKGENNKKKTYYYSPAEKEFQ
                     SLIVENLKKKYFLIYQEETEINNFEITPIDINTSNLKIINYKGTIIKGWMGKFKIKGE
                     GEILKVAYDCGIGSKNPQGFGCFELLLFSEKEVRG"
     gene            42686..42994
                     /locus_tag="PLW95_01430"
     CDS             42686..42994
                     /locus_tag="PLW95_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21330.1"
                     /translation="MENKNKKTIPEYVKKLFWDVRKEDLDIEKHSTFIIKRVLDYGDV
                     PSLKWLIRIYPDYLIKKVIHNKKGLHPKTINFWEKHYEKRRVLKWLEYNDLELKTPEE
                     "
     gene            42998..44944
                     /locus_tag="PLW95_01435"
     CDS             42998..44944
                     /locus_tag="PLW95_01435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02556 family CRISPR-associated protein"
                     /protein_id="HOV21331.1"
                     /translation="MIEAIKEIGEKILSNTSPDKFLENLVEVPKKINGEEQYIVIIDF
                     NTDEETVTFDFEEMKDDTPKKYLWIGNALSNNPQDRFTTNNLEYLISQTIPNVIEAIS
                     DGELKKLLEKVKNKFYYDLGDHSKQNKKYQYILDIEKIRISSKSIEQILEEVNNNPKK
                     LVNTVKNEIFEYLKREKNLTKKQIGLFNLKIDNQLISDFPEYRKHLEDSLIKKLFSKT
                     IRGRCFLCQKEKEVTKDMRFKFSYYITDKISFASELEEKGFLKNFTLCEECYKKILVG
                     ESFIKNKMRTKVGLNLYIIPQFIFGDISFPIKKLDKWAEYIKQSLNSVVNLEGLRKFQ
                     DRLEEYSEFQQHKNNFILNLLYYRKYQSEFKILKLILDVPPSRLDFLKEKENEIYEIA
                     KKIFGEDNRWYLNLERMYYLFPVRISKQEIGDYKKVLDFYDAIFSTKSISYKFLIKQF
                     VDLIGVYRFGKFDSYNIKKPKDTDTGLIYSVLEANLLLLYLKKLKLLQRGGDNMNNID
                     TSLLSEGMKKYIEEMGYGEEQSALFLLGYLIGEIGNAQWSKENPNKPILNKLVYQGMD
                     VKKILRLTNEVFEKLKQYKRNILYNEQIFAECKRLLDKNLNNWQLSNQENVFYILSGY
                     AYNMLKKIDENKLKENKNEEVENE"
     gene            44937..45914
                     /gene="cas7b"
                     /locus_tag="PLW95_01440"
     CDS             44937..45914
                     /gene="cas7b"
                     /locus_tag="PLW95_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013401700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas7/Csh2"
                     /protein_id="HOV21332.1"
                     /translation="MNEEKIKNSDIIFIYDAKLCNPNGDPDDENKPRMDYQTSRNLVS
                     DVRLKRYIRDYLYAQGYEVFVSKIDDMTVDATKRVEKLFENYEENGKKGGVEINKLEE
                     KHIKWLLQRVIDVRMFGATMPIKAKKGIAKGASNAFTGPVQFNWGYSLNKVQIVESST
                     ITSMLAGRGEEYSTMGKDWRVYYSLIAFYGIISGKRAEKTMFSEQDIKILDNALLKSI
                     PQEATTRSKIGQKPRLLVRIEYIDNTTFLGDLRRYIKIEPDDNLRDINEFELEVNKLV
                     NLVTNNKSKINKIYLWQDDDLKLKEGQIFKELIENGFKDKIIDLPHSEE"
     gene            45919..46728
                     /gene="cas5b"
                     /locus_tag="PLW95_01445"
     CDS             45919..46728
                     /gene="cas5b"
                     /locus_tag="PLW95_01445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5b"
                     /protein_id="HOV21333.1"
                     /translation="MEYKIDRLLIFDIKGAVAHFRKFYTNSSSLSYSFPPRTTITGLI
                     AGILGRERDQYYEEFSTEKCKIAISIRTPVRKIMQTVNYIQTKSDKGGIKGVNLSAGH
                     TQIPLEIILPDRGFEELKYRIYFYHKEQVFEQLKKVLKEKKFVYPPYLGLSEFIGELE
                     FIDCLEETDIMVYASKKDTTTDLVTVVNTEKIQEGGLIFEEDSGKILQYIKEQMPLEF
                     DSQRNFKKGANFLYEKNNSNIKLKIKENYYKINYNNSEKKEIEENIVFMES"
     gene            46729..49146
                     /gene="cas3"
                     /locus_tag="PLW95_01450"
     CDS             46729..49146
                     /gene="cas3"
                     /locus_tag="PLW95_01450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HOV21334.1"
                     /translation="MKFYSHIGEKGGKKELKHHLKGVGEESRHIICELPIKDKCFFSD
                     ISYLIGISHDFGKYTSFFQEYLINQKENKLSQHSFISAIFTAWQIQEYLDVNNNFKSY
                     SYIPLIGYFIVLHHHGDLRSIGEDIPSYKDFKNPPCFSYAEPRTREKLQMTYKQIEDF
                     KRPNRISIIENEYKEMVGASDIKDFLNTWLSILKKLNELRQNYENEGDGIKIALFLFT
                     LLLYSILIDSDKRDAGNVSIIKRKKLMPDLVDRYREKQFNLCSRKTMDIIRNQIYKES
                     ILKIEEVSLENHLYTLTAPTGSGKTLTSFSVALNLRTRIEKEKGYMPRIIYSLPFITI
                     IEQNYEVIRNVLCSEIEDFKENETAYILKHHYLSDLEYKEGDELKPLDESLLLIESWN
                     SEVVITTFIQLLHTIIGFKNSFLKKYHNIAGSIILLDEVQNIPIEYWDLVEKVIKIVA
                     EYFGCYFILLTATKPLILKEEYATEIVKNPKTYFEKLNRVLIKADLEKKNIDDFIKWF
                     IENYNQENSYLIVANTIKSSKEIYEKIKTYKLKNPLFYLSTSIIPKHRAERIKKIKSL
                     LDKNNIPILVSTQVVEAGVDIDFDVVIRDIGPLDSIIQVAGRCNREYTKDKKELYVFS
                     LGQFASYVYGKIHPEISRELLNAKEIPESNFYDLINNYFEKVKFKINDDVSRYIWESM
                     LELRFYEKNPPVRNGFEKMVSAFQLIKEKGEYIDIFVEIDEDAKNIWERYCKKIYEER
                     DFLIRRNNYLKIRKEFRSYIISIRNYKDIFLPSEVCGMRYIPKNEIKNFYSPETGFCS
                     EIDTLIF"
     gene            49174..49671
                     /gene="cas4"
                     /locus_tag="PLW95_01455"
     CDS             49174..49671
                     /gene="cas4"
                     /locus_tag="PLW95_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012996176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HOV21335.1"
                     /translation="MEFKIIGTFVWYYFICKREVWFMAHNIQPSQENPYIEIGRLISE
                     EFYPREKKEIKLESMIIDIIKCKDGQLLIGEVKKSSKYLEAAKMQLLFYLYQLKEKGV
                     EAKGELLIPKEKKRIEVVLNEENERKLLLDIEKIKNIISQNKLPEKVKNQFCRNCAYD
                     EFCWS"
     gene            49677..50672
                     /gene="cas1b"
                     /locus_tag="PLW95_01460"
     CDS             49677..50672
                     /gene="cas1b"
                     /locus_tag="PLW95_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1b"
                     /protein_id="HOV21336.1"
                     /translation="MKKTIFVFSDGQFQRKGNTLYFETKEGEKKYLPVENISEIMVMG
                     EVDLNKRFLEFLSQTEIILHFFNHYGYYIGSFYPRQHYNSGYIILKQAEFYNDERKRI
                     SLAKKIFNGAVENIEKVLTYYSNRGKEVGGYIKEIENLKEKVDNCNSIEEIMAIEGNI
                     REHYYSSFDKILEDEEFIFEKRSRRPPRNKLNTLISFGNSLLYVAVLSEIYKTHLDPR
                     IGFLHTTNFRRFTLNLDIAEIFKPIIVDRIIFTLVNKKIIQQKHFEDKMGGLILKDPG
                     KQIFVEEFDNRLRTTIKHKGLGRNVSYRRLIRMELYKIEKHLIGEEEYKPFVSGW"
     gene            50673..50939
                     /gene="cas2"
                     /locus_tag="PLW95_01465"
     CDS             50673..50939
                     /gene="cas2"
                     /locus_tag="PLW95_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003871087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOV21337.1"
                     /translation="MYAILVYDINEKRVSKALKICRKYLYWVQNSVFEGEITFPKLEK
                     LKSELNKKVIKSTEDSVIIYILKDERYTKREILGEKKVNKGIIL"
BASE COUNT        19426 a         6819 c         7788 g        17316 t
ORIGIN      
        1 agaatttctt attctgaata taataaagca aaaaaggaaa ttttacaaag attatttcaa
       61 ataaaataaa taaggaacgg gtcaaaagta tttattttgt tagaggtttg gttaataata
      121 gtattttgtt tagagaaaag aagaagatag gaataaaaat agaaacattt ggtgatgaag
      181 cattatttac tattgaacca atttcaaaag atgtaaaaat tgatgaaata attaaaaaat
      241 ataaactcat tacttataaa gaatacataa aaagggcaac agaagttcaa gatgatgcaa
      301 gaactaaata gtgtttttat aaatgttttc tacaagttcc attgttttaa ctgcatcata
      361 aaagtttgtt tctggtaatt tctttttctc aatacattca ataaagtgtt tgttttcatc
      421 taaaaaacca gcaatttcaa ataattcttt tcctcctgta aactcttttg catctatact
      481 ctctccttct tcttttccat cagaataaat cattgcttga ttttcagggt caacaaatac
      541 aacaatccct ttaccatgta tttctaaaga aaaaaccctt tttccactcg tccagtttgc
      601 agataaaatc cctattgctc ctgaagaaaa ttcaattaaa gaacaaaaaa tgttattata
      661 atcagcaaat agccctttaa caatactttt aacttttttt acatctcctc caatatacct
      721 taaaaggtca actgagtgaa taacatcgca ggtcaaaata tcaattgccc cattgtaata
      781 aggtggttgg cccaaataat ttttaaaaaa atttgcctgt gttaatataa cttccccttt
      841 ttcttctatt ctttttttcc cttctactat taaaggggca aatctccttt gaaaaccgac
      901 cattgtcaaa acaccctttt tttctgccaa ttttgccatt tgttttgtct gttcaagtgt
      961 tattccaggt ggtttttcta taaaaacatt aaggcatttg tttaaacaat gaataacgat
     1021 atcaaaaaga tggtgtggtg gcattattat atagatagca tcaggacttt tttcttcaag
     1081 cattttttta tagtcagtat aaacatttga aattttatat ttttctgatg tttttcttaa
     1141 tcgttcttca ttcaaatcac atatacctat aatttcagca ttttcaattt ttgaaagtga
     1201 aggataatga actaaatttg ccattcctcc tgctccaata atacatactt tcaccatttt
     1261 tttgaccttt caataatttt ttttattttt tcaactgttt cttcaatatc tttgtttatt
     1321 actctataat tataatactt catcaatatt ttcctttcat attttgctaa ttttattctt
     1381 ttctttattt cctcttcact caggtcacct ctttttctca atctttcttc ctgttctttt
     1441 atagaaggag gtaagatacc aattaaaatt gcatctggaa atatcttttt aatattcatt
     1501 ccaccttgag tatcaatcac aagtaagaca ttttcatctt tttttaattt ttctattact
     1561 tcttttttgg gtgtaccgta gaaatttcca taaacatttg catattcaag aaattcatct
     1621 ttttcaatca tttttttaaa cttttctata ggcacaaaat tataatcaat tccatccttc
     1681 tcaccaattc ttggttttct tgtagtataa gttataacag ttttaagata tggtaataat
     1741 ttaactaatt gcttttgaat agtggttttc ccagttccag aaggagcaga aataacaaaa
     1801 attttgccgc ccatttatgg agtaaatttt ccttttgttt ctaactcttg tattgctctt
     1861 tcagcaatac ttttgaatcc agtatttaat aacttttccc attgcttcat agcaaggtca
     1921 atttgcccat ttttctcata tgcatgtgct aaaacatcat caacataatc aggatgagga
     1981 aattttattg ctttttctaa atacctgaca gcatttttgt aatccttgcc tttgtgataa
     2041 tatgtccatc caagttcaaa ataaaggtca tatttttcag gattataggc aacccctttt
     2101 tttaaaaatt caattccctg attgtaccaa taaagcatct catcaactat acctataaat
     2161 tcttttaagg catcaataaa aaatttattt tcctcattta ccttataatt atccaaaaag
     2221 tactggtaat tacccaactc tgtaagcaat ttcgttattc tatcttcctc catcccttct
     2281 tttaatccca aattctcaat caaaaaattt aatttctccc ctattttaat tatgttatgt
     2341 aagtcctttt tatctttttc aggtaaattt ttaacttttt ctttatactc ttctatcaag
     2401 ttttctttat taattttaac ctgggcaaaa atattgtaag acatatgcca accaccaaca
     2461 gaccaaacag taacatattg gggttgtaac catgcaattg attctaaaag aggtagcatt
     2521 ttatagtgct gtccttcgtg ccaataattt tctatattta accataatag gtctgctgcc
     2581 aatcccttaa aaccagaaag aactaaactg cctgctacct gtcctggcat aagtaatagt
     2641 ccactttcaa gattttctaa ttttttatta taatcaatac tcacctgcaa aaatcctaat
     2701 ggaatgtata ataaaattgc agtaaaaaga aaaacccagt ttttactatt aattttgttt
     2761 tttctcatac ttccctcttc tggaagaaat aaagaccaat aagtataaca atgaatgtat
     2821 ataaaatccc ataagttaat gtctttaaaa cataactcca tgaaacagga actccaagta
     2881 caactttatc tcttatcgca aagttttcat aatttggaac aattgtataa agcatctgac
     2941 ctaaaccttt taatataaca ctttctgttc tatcaacaag ttgtcttcca taagaagtta
     3001 aatgaccaat tatataaagg aaaaaggaaa aaataatgtt aaaagcatct gatgcaaaag
     3061 tagcaacact taaagttatc gtacttatta aaacaagttc aaaatatatt aaaagaagtg
     3121 ttttaaatgt ttcaacatct ggtgggcttt ttttgaaaac aagaagccct ataaaaaaca
     3181 aactgataag aataaaatta actaaaatta tcaaacacat acctaaaaat tttccaatga
     3241 ataaatttct tcttgtaact ggttttgtta gaagtgtata aattgtcctt ttctctattt
     3301 cccctgataa agcacccaaa gcaccaaata ttgcaattaa agcaccaaaa aattctattg
     3361 tagaaaaact cacatcttta atcattttaa gttcttcttc tgctgtgaga aaagaaaaga
     3421 attttgaagt gcctattaca atcaaagcaa aaacaacaag tatatacaat gttttttttc
     3481 ttaatgattc tttaaatgta ttaactgcaa taacccatac ttttctcatc cctcactcct
     3541 ttttataatt actatcaaat tcttctattg ttttaacaaa aagttcctca aggttgtttg
     3601 cattatattg agaaatcaat ccttcaagag tacctgttgt aagaagataa cctctatgaa
     3661 aaatacctat tctactacaa actctttcaa cttctgaaag aaaatggctt gaaagtaaaa
     3721 ttgtttttcc ctgttttttt aactgaatta ataagtccct tgtttctagg catccaatcg
     3781 ggtcaagccc tgttgtcggt tcatcaagta taagtatttt agggtcattg ataagtgctg
     3841 ctgctaaacc aattctttca agcatccctt ttgaataatg tcttaatgtt aattttttta
     3901 cttctgataa tttaacaaga gaaatcaaat attcagttct ttcttttatt tcctctttat
     3961 tcatattaaa aagccgtcca taaaaataaa gaagttcagg accagtaaga tagtcataat
     4021 aatagggatt ttcaggcaaa aacccaatat tttctttaac ttcaacatca aattgatttt
     4081 tccccattat acttatttct ccttcagtag gaaataaaat tccaagtaaa agtttaagac
     4141 atgttgtctt tcctgaacca tttggtccaa gaattccgaa tatttctcct tcttcaactt
     4201 ttaaacttac tttatttaaa cctactactt ctttccctct tccgagaaca taaatttttg
     4261 taaggtcatt tgtctctata attgccataa aacacctcca atttgtttca ttagtttata
     4321 ccaattaaga acaattgtca aatattcttt taatctaatc taaaaagttc atcaataata
     4381 gacagaagag aagttccttt tttaccaaat aattcaatat cttttttaag ttgtttatgt
     4441 ataaaagata atttactttt taattttttt atatcacctt ctttatcttc tatatcatct
     4501 cttatctgaa atccaattcc aaaattttta gaaattttta aaagttgttt tttctcttta
     4561 tcatttactc ttttgaaaaa agttgggccc aaaaaacaca cctcaaataa tttacctgtt
     4621 tttttttcat ctctttgtct tttaaattta tcgctaacat ttttatcctt ggaaataata
     4681 tcaagaacct gtccacctgc catgccttct gaaccaattg cttttgcaac ataactaata
     4741 agagaagata tttttgtccc tgcaataact tgaaaaccaa gagcaataag tgaatcacct
     4801 gctaaaattg ctatatcttc tccaaatttt ttatgacagg taagttttcc tcttctataa
     4861 tcatcattat ccattgcagg taggtcatca tgaataagag aaaaattatg aataaattct
     4921 actccacagg caaaaggcat caattttctt ttatcaatat ttaaaatttc tccaacaaga
     4981 agaattatta taggccttaa tctttttcca cctgaaaaaa tagaatatct cattgcttta
     5041 tgcaatatag aaggtgttat tttttcagat ggtagatatt tatcaagata atattcaatt
     5101 tcctttttta atttttttat tttatccatt atttataaac tttttgcatc cgtctggcgc
     5161 caaaaccaca taaaatttcc tgtggaacgg tattacctat ttttgccata ttttcaattt
     5221 caaaattatc accaaaaata tatatttcat ctcctatttt aacatttttt ccacttacat
     5281 ctacaagtgt ctggtccata cagatattac caattatctt tatttttttg ttattccatt
     5341 taagctcaaa cttgtttgag agaaatcttc ttagcccatc tccatatcca atgccgagtg
     5401 ttgcaacttt tgttttcttt tctgtcttat aagtaattcc ataacttaaa tatctccctt
     5461 ctggtaattc tttcaaaaaa aggactttac agaatccctt taatgctggt tggagtttta
     5521 atttctttgc aagttctttt gaaggataga ttccaagagt taatagtcct attctaatca
     5581 tatcaaaatt cttatatgtt tcaggcaggt ttattattgc tgcggaattt gctatatgtt
     5641 tcaaaggtat atttttaagt tcatttactt tttcaagaac actgtaaaag tttcctattt
     5701 ggtattttgc atattcctta ttttcccatt cagcagttgc aagatgagta taaatccctt
     5761 tggtaaaaat gtttttttct tcttttactt tttttataaa attttctgct tcttcttttt
     5821 gaatacctat ccttcccatt cctgtgtcta tttttatatg aacaactgcc tttttttgtc
     5881 tttttttaga aactcttttt aactgctcaa aaacatcatc actgcataat gtaatttcaa
     5941 tatctctcat tataagttct tcaacatcag aaggtaaaat tggtcctaaa acaaggattg
     6001 gttttttaat tccaatttct cttaactcaa taccttctga aggtattgca actccaaaat
     6061 aatcaactac actttcaatt ttttttgaaa catattttgc acccatccca taaaaatcac
     6121 tttttactgt tgccataaac tttctttttg taaaattttt tatctttttt atattttcaa
     6181 ttatttttcc acaatcaatt tcaatccatc tatccatttt ttatctcctg taatattttt
     6241 ttaacacaat cttctggttc tttactatca taaacaggtc taccaacaac aataaaatca
     6301 gccccttctt ttactgcctg tttaggagtt attattctct gctggtcgtc attttccttt
     6361 tttaatctca ctcctggaac aacaagtaaa taaggatatt ttctcaaaac agaaatttct
     6421 tctcctccaa ctacaactcc atcaagtccc caattaaaag cattttctcc taatattgaa
     6481 acttttttct ttatatttga aaatccaaag tcattttctg ttaaagatgt taaaacagtt
     6541 attccaataa gttttgtaat tttgtttgtt ttctctctct cctcaattgc ttcttttatc
     6601 atttcctgtc cacctaaaat atgtagagaa atcatatcaa taccaatatc acagcaattt
     6661 cttactgcat tttttactgt attaggaata tcaaaaaatt taaaatcaaa aaaaattttt
     6721 ttgtttttct tttttaattt gtcaattaaa cagaaaccaa attttaaaaa agcaactgga
     6781 ccaattttat accagccaac ataattttct gttttttcaa ttataaaatc cattttttct
     6841 tcttctgtta tatcaagaga tattattaaa tttgccattt tttctcccca caagttcaag
     6901 tatacctact attgaaagaa ttattataac ctgaattata ataattaaac atccccatat
     6961 gtttaaattt ttcatcaata acgctccaag tcctgtacaa attgtcagaa aataaataaa
     7021 aataactgcc tgttttttat tcatccctaa attaactaaa cgatgggaaa tatggttttt
     7081 atctgcttta aatataggta attttctctt ttttcttatc cagataattg aaattgtatc
     7141 aaaaaatgga acagaaaaaa taacaagagg agcaaaaatt ggcaggtatg gatttgttgt
     7201 ctcatactta taaaaagtaa taataattga aattgttgca agaaaaaaac ctaaaaaact
     7261 acttccagat tctcccataa aaatttttgc aggagggaaa ttataaatca aaaatcctga
     7321 aatagcccct aggaaaacag atagtaatgt acttacaaaa agttgtccca tctgtactgc
     7381 aaaaataaaa agtataaacc cggaaattaa ggaaactcca gcacttagtc catccatatt
     7441 atccaaaaga ttaaaactat tcatcataaa aatgacccat ccagttgtta taataaaaga
     7501 aataaaattg ttaggaatga aaagagaaat ttttattcct gagaaaaata aagcaattga
     7561 tacaaaaatt tgtaaggata atttgacaaa tggcttaaga tggaaaatat catcaaaaat
     7621 accaaaaaga acaacaataa aaccaattga taaaattaac aaaagttttg gtaatgactg
     7681 aataattcca ggaatataaa ttttcaattg taagaaaata atatttaatc ttacaaatag
     7741 aatacctaaa agaacagtag caagaattga aagaaaaatt ccaagaccac ctaaaagagg
     7801 agttggaact ttgtgaatct ttctttcaga aggtatgtca ataatattaa attttctagc
     7861 aattttcccg gaaaaaggag taaaaaaaat tgacaacaaa aaacttccta aaaaacccaa
     7921 aagataaaca acattccaca taaaataaat tataaaagaa aaaagaagtt tttcaagaca
     7981 taagtatttt taaccattta aatttcagac aataaataaa atgccaaatt gaataaaatg
     8041 gtgtataata tgaatattct aagaggaaca gcaaaaaaga taattcagta aaaatatcgc
     8101 aggtattaaa gatggaaaag gaaaaagatg agaaataata aaagaggcgg gattacttat
     8161 gcagatgggt ttttatcttc tggtatttat agtggaataa aaaaagaggg aaaagacctt
     8221 tccttaatat attcaaaatt accttgtgta gcagttggaa cttttacaac aaataaattc
     8281 aagtcatata gtttgatagt aactatgaaa aatataaaaa atccaataca ttctattatt
     8341 gtgaatagcg gaaatgctaa tgcttgcaat gggaaagaaa attatataaa aacaaaagaa
     8401 gtagttggaa aattagcaga aaaattaaaa gtaaaagaag aaaatatact ttttggttca
     8461 acaggtatta tcggtgctcc tttaccctat caaaaaataa tttcttctct tgatgaactt
     8521 ataaattcat tatcttgttc tggacatact gccgcagcag aaggtataat tacaaccgat
     8581 acttttttaa aagaatttca gataaatacc ggtataaaag gaagaaaaaa agaaattttt
     8641 attgggggta tggcaaaagg agcaggtatg ataaatccag atatggcaac aatgctgtct
     8701 tttataacaa cagatgcagt tattgaaaga gatgcacttg aaactgcttt aaaacaagca
     8761 gtagataagt cattcaatat gttggtagtt gataatgata caagtacaaa tgatatggtt
     8821 atatgtttag caaatggagc agcaagaaat aaaaggattc attataatac ggatgaatat
     8881 tttaaatttt accagtcatt agaaaatgta tgtataaact tagcaaaaat gattgctaaa
     8941 gatggagaag gagcaacaaa attcattgaa gtgaaagtta aaggtggctg gtgcgaaaaa
     9001 gatgtaaaga aagttgcgaa aaaagtgtgt ggttccaatc ttgttaaaac agcaatttat
     9061 gggtgtcaac caaactgggg tagaatcatt gcatcagttg gtagttgtaa agtaaagttt
     9121 aatccagaaa aattagaact taaaatttgt ggaataaaca tttttcaaaa tgggaatgta
     9181 attaattttg ataacaatct actgagacaa aaattacagg ataaagaaat tttgatagaa
     9241 attgacttaa aacagggaaa ataccagaca tcttcttttg gatgtgatat gacagaaaaa
     9301 tatataaaaa taaacaagga atacgaatga ttgctaaaat gttgaagttt catctatttt
     9361 ttcttggaga tgaaaacaaa cttttaagaa cactacaaaa aaaaggtatt gtggaaatta
     9421 accaattaga aaaagaatat ggttttgaaa atttaaaaga gagggtaatt tccattgaag
     9481 aaaaaataaa taaaattgaa tttcttaaag gcattgttaa aaaaattgat ggtaaagaat
     9541 ttagtggaaa aattttactt acagaaagtc aggaacaaaa aataatatct aatttcttag
     9601 ttgatgaaac atttgataat ttcaaaaaat taaatgatga aattgaaaga aggaaaatag
     9661 tggataggaa aatagaaaca ataataaacc aactgaataa atttaaaaat ataaaattaa
     9721 attttgcaac tcttttttct ttgaaaaatt tttcttttta catttttaca tcaaaaaaac
     9781 ctatttcttt aatatctgaa gatattcttt ttgaaaaagt atatcaggat aaaaaagaaa
     9841 tattctattt aattgttttc ccttctggaa agaaagaaga aatttttgag aagataaaag
     9901 aaaataaagg agaaataatt gaagtaaaaa agtggaataa atatccagaa gatattataa
     9961 aaaaacttct aaaaacaaaa aagaaaaatt tagaaaaaat taataatttt gaaaagtata
    10021 taggtgaaaa tataaaaaaa attaagaatg aaatctttgt tatttatgac cattatattt
    10081 cattgctcga ttatttaaaa acaaaagaga aagttacctc ttcaaaattt ataaaatgtt
    10141 tttgtggctg gattaaagaa aaagatattg aaaaattaaa ggaggccatt aaagaaacat
    10201 caccagatag ttttcttcat attgttccac cacaaaaaga tgaagatgta cctatagtac
    10261 ttgaaaatcc gcccttaatt gaaccatttg aggttgttac tgacctatat ggaagaccta
    10321 tttataaaaa tatggaccca acatttgccc tttctttatt ttttgttatt tcttttggtt
    10381 tctgtacttc tgatgctggc tatggaatat tactgatttt gctttcttta attttaatga
    10441 gaaaatttaa gttctatcct tcttttatta aatttcttaa acttcttata tatggtggga
    10501 taggggctat aataattggg gctcttacgg gaggatggtt tggagatata ataagcaaat
    10561 taccagaaaa gtccattatt acgaaaacat taagttcatt tataatttta gacccattaa
    10621 aagaaggaaa taatgcttta atttttcttg catgggcttt aattattggt tatattcaaa
    10681 taatctgggg tttgatatta aatctttata attcaataaa aaacaaagta ataacagata
    10741 tattacttgc aacttcaact ctttcagtac agatttttgc aggagtcctt gttttccttt
    10801 tcttaaaagg gctaaaaaca ggtatatttt tttatattcc tatttttatt gttattctaa
    10861 attttctgac aattatggct gtaaatgcaa cctcacaaaa ggggcttctt ataaagggtt
    10921 tctgggctat ttatagtgct tattcagtta ttgcaggtaa cttattagga gatgttttaa
    10981 gttatagtcg tctttttgga cttggattaa catcaactgt tcttgctatt gttgtaaatc
    11041 aaattgtatt tatggcaggt ggtattccat ttataggacc tttatttgct gttataatat
    11101 ttttgcttgg acactttggg aatcttgcaa taaacttatt gggtagttat gttcacacaa
    11161 gtagattaca atatcttgaa ttctttacaa agttttatca gggaggagga aagccattta
    11221 gcccattttc agagaaacgg atttatacat atcttaaaaa tttttaattt taagaaaaaa
    11281 aaggtaaaat aaatggtagt gtcaaaaaat agatgaaaga aagatgaaat tccccctcac
    11341 ccatacccct cgggcgtagt tcatatgctc acccgttggg ctatgcccag agggatccgt
    11401 agccaaaagc gaggacccta cgggcgagcc tctccccaag gggagaggga agaaagaaaa
    11461 aagaggaggg atataaagac ttataaaagc attaaaagac aaagaaaaga actataaaat
    11521 ctatagccaa ttttggcaga accaaataaa tgaaaaggag gagaatatgg gaaaagaaag
    11581 tatatggagc ccgacagtgg cattggcaca gaaattctgg ctcggactgt caaaagtaat
    11641 tgcagcagtg ttaatttttc ttattggatg gcttattgca cgacttttaa aaaatttgat
    11701 tataagattt tttaaggtaa taaaaattga taagttgggt gaggactcag gtcttaaagg
    11761 actccttgaa aaaggaaatg ttacaagaga catttctgaa ttgattggta ttggtgtgta
    11821 ctggcttttg atgcttgtag ttatctttat tgcagttgac tttgccggta ttttaattcc
    11881 agaggaagtt ataaacactt tacttggttt tataccaaaa ttcatcttag ggcttcttat
    11941 ttttatattt tctgtttttc ttggaaattt tttcaacggg atagttagaa catcagcagg
    12001 aaatatggga atagaaaaag catctcttct tggtaaagtt actcatattt caattgttgc
    12061 atttggggca gttattgctt tacaacaaat aggaattgct cccacattta tagcaaatgt
    12121 ttttataatt gttttaggtt ctttctgttt tggaattgct cttgcctttg cacttggttg
    12181 taaagatatt atcaaaaatt atttagaaga ccattttaaa aaggacaaga aggaataaat
    12241 taaagggcgc gtagctcaag ggcagagcac cgtgctgata acgcgggggc tggtggttcg
    12301 aatccacccg tgcccattaa aggagaaagt tatggaaaaa attgaatttt ctgctgaaat
    12361 aagagaagga acaggtaaaa aaataacaag aaaattgaga aaaaatgagt atatccctgc
    12421 tgttctttat ggagcaaaag agcccccttt gttgctaaaa ctaaaaagaa aggacacaga
    12481 aaaaacaata agacacatag aatcacatag tattatggca gacctgataa ttgatgaaaa
    12541 atcagggaag aaagaaaatg tgaaagttgt attaaaagaa attcaggtga accctataaa
    12601 ggaaaatatt acgcatcttg atttttatag agtaagtatg gataaaccaa tattcattac
    12661 agttccactt cattttatag gaactgctcc aggtactgaa aaagggggaa ttcttgatga
    12721 agaaatgaga gaaataacaa ttgaatgcct tccaaaagat gcgccttctt ttcttgaaat
    12781 tgatatttct tctcttgata taggcaacac attacttgtt aaagatatta aaacagacaa
    12841 aaaaataaga atagttgaaa atggagaaag ggtagttata tctgttcttg ctccaaaagt
    12901 tgttgaagtt gaagaagaag cagtagaaaa agaagaggtg aaaaaagaac cagaagttat
    12961 aactgaagaa aaagttgaag aaagaagaaa agaaaaagaa aaggaaaaag aagagaagtg
    13021 aaaatttttt ttggtattgg caaccctgga aatgagtata agaatacaag acataatgcc
    13081 ggcttgatgg taattgagaa ttttttagac aaatttgaaa attatacaga aaaaaaacat
    13141 agatatttta tttcttatga aatttctctt ggcgggaaag aaacaatcct ggtaaaacca
    13201 agaacatata ttaatgaaag tggaatatct tttaagaaag ttcttgatac atataaagta
    13261 gatgttgaaa atgtaattgt tatccatgac gatttaggaa tagagacagg aaaaattaaa
    13321 attgtatttg ataaaggaga taatggacat aaaggaataa tttcaatatt taatacagtt
    13381 gagacaaaaa atttctaccg tataagaata ggaataggaa aggggaaaat agaaataact
    13441 tatgttgatt atgttctttc tccttttgaa gaagaagaaa aagaacttat aaaaagttca
    13501 atagaaaaag cagtaggtgc aatagaagaa ataataaggt caggaattga aagagcaatg
    13561 acaatttata atagataagg aaggaggcaa aaatggattg gtctcaggta attacaatta
    13621 ttggttctgt ttttgcattg ggatatactg tttttattgt aaaagggata ttaaaagaaa
    13681 aagaaggaac tcctgaaatg atagaagttg cagaagcagt cagaacagga gcaagagcat
    13741 atttaaaaag gcagtataaa ggcgttagca tgttttttgg gattgttttt ataattcttc
    13801 ttcttcttgc ttttttgaaa tatctgccta tttttgttcc atttgctttt cttacaggag
    13861 ggttcttttc tgctttaagc gggtatattg ggatgacagt tgcaacaaaa tcatcttcaa
    13921 gaacaacctt tgctgctaag acatctctaa ataaggcatt aagaattgcc ttttctgctg
    13981 gttttgttat gggacttgtt gttgttgggt ttgctttatt tgatataagt atctggtata
    14041 atattttgaa ttttgtttat agacacctac ctcttgaaga aaaattaaaa aatataactt
    14101 caactatgat atgttttgga atgggagcaa gttctcaggc actttttgca agagttggag
    14161 gtggaattta tacaaaagga gcagatgttg gagcagacct tgttggaaaa gtagaggcag
    14221 ggattccaga ggatgaccct cgtaatcccg ctgtaattgc agataatgtt ggagataatg
    14281 ttggagatat agcaggtatg ggagcagacc tttatgaatc atatactgat tcaataattg
    14341 caacaatggc tcttgcttat gctgctggac tttcaattaa agggattgca gttcctatgg
    14401 taatagcaac agttggggtt atatgttcaa taattggttc tttatttgtt aaagttggtg
    14461 aagaagcaag tcaggcaaac ttattaaagg ccttaagaag aggaacttat ttagcatcaa
    14521 ttcttacatt tattatttcg tattttgtaa ttattaaaat tcttggcagg ggatatattg
    14581 gactttatgg ttctgttata agtgggatat tcgcaggtgt tataattgga ttttttacag
    14641 aatatttcac atcagacaat tatagcccaa caagaaatgt tgcaatagca gcaaaaacag
    14701 gacatgcaac tgttataatt gaaggacttg caacagggat gttttccact attgctcctg
    14761 ttttaacggt tgtagttgca actttatgtg cttatatatt accaggcgga tttgccgacc
    14821 ctgctcttgg gctttatgga attgggcttg ctggtgtagg aatgttaagt acacttggaa
    14881 ttacacttgc aactgatagt tatgggccag ttgctgacaa tgctggtgga aatgcagaaa
    14941 tgacacatca accatctttt gtaagacaaa gaacagattc tcttgatgcg cttggaaata
    15001 caactgctgc aacaggaaag ggatttgcaa ttggttctgc tgctttaact gctcttgcct
    15061 taattgctgc ttataaagac caggtagttc tcttaggagg aaaaattgat ttttcaatta
    15121 tgaatgtacg actcctttta ggtatcttca taggagcaat gtatcctttt ttcttttcat
    15181 caatggcttt aagagcagtt ggaagagcag cacagaaaat tgttgaagaa gtaagaagac
    15241 aatttagaga aattaaagga cttattgaag gaaaagcaaa acctgactat gcaaaagcag
    15301 ttgatatatg cacaaaagca gcacaaaaag aaatggttat tccaacagtt ttcacggtca
    15361 ttttaccaat tattgttgga atacttttag ggacagaagg agttattgga ttgcttgtag
    15421 gggcagtaac aagtggtttt gcagttgcaa ttatgatggc aaattctgga gcaagttggg
    15481 ataatgcaaa aaaatatatt gaaaaaggga atttgggtgg gaaaggaaca gaagtgcata
    15541 aggcagcagt tacaggtgat acagttggag acccatttaa ggatacagca ggtccatcat
    15601 taaatattct tataaaatta atctcaatta tttcaattgt ttttgccagt tttattgtca
    15661 aaaattctct ttttaaatag aaagtgcgtt tttccatccg ttcatctttt tggaggatgt
    15721 aaaattataa ctaaaagcgg aaaagttaaa gatacaactt aaaatttaaa aagtttttat
    15781 cttttgtatt tcgtccgcta aaaagcgcat cggacgctaa aatacgcgtt ttgaacttta
    15841 cattttagtt ttacacttta cgctttacat tttacatttt taaaatattt attttaaata
    15901 aatgaaaaat atatttgaaa agtcaattca actacataaa aaagcaaaag ggaaaattga
    15961 aataaaatca aaagtccctt tactagataa aaatgaccta tcacttgcct ataccccagg
    16021 agtagcaggt ccctgtgaag aaataaaaaa agatatttca aaaatttata aatacacttc
    16081 aaaatggaat ttagttgcta ttgttactga tggaagtgct gttttagggt tagggaatat
    16141 tggagcagat gcatccttac cagtaatgga aggaaaagca attttgttta aggaatttgg
    16201 tgatgttgat gctttcccaa tttgtataaa atcccagaaa attgaagata tagtaaaaac
    16261 catatctctt atttctcctg tttttggtgg tataaatctt gaagatattt cttccccaag
    16321 atgtttttca atagaaaaaa aattagaaaa aatacttgat atacctgtct ttcatgatga
    16381 ccagcatgga actgctgttg ttgttttagc aggacttata aatgccttaa aattgactaa
    16441 taagaaattt aacaaaatta aagttgtaat aaatggagca ggagcagcag gtattgcaat
    16501 aacaaaaatg ctttataaaa caggagtaag agaaatattt ttatgtgata gagttggagt
    16561 aatttataaa ggtagaaaag aaaatatgaa ttctgcaaaa gaagaaataa ctaaatacct
    16621 atcaaaaaaa agcaaaaagg gaacactcaa tgaagtaatt gaaggaagtg atgtttttat
    16681 aggtgtttca ggacctgata tattaaatga agaaatggta aaaaaaatga ataaaaattc
    16741 aataattttt gctatggcaa atccaatacc tgaaataatg cctgataaag caaaaaaagc
    16801 gggtgctttt attgttggga caggaagaag tgattttcca aatcaaataa ataacctact
    16861 tgcttttcct ggtattttta gaggtgcctt agatataaga gcaaaaaaaa taacagatga
    16921 aatgaaatta gcagcaagtt atgctattgc aaattatatt cctgaaaata aactttctcc
    16981 ttcttatata attccgtctc ctcttgataa aaaagtagca aaaaaggtct ctgaaaaagt
    17041 atcttctgcc tatttatgaa attgccttaa tttttgaaat ggttgagtat attttttctt
    17101 ctgtattttt gttcactttg cttgccatcg gtaatgtttc ataacttgta tttatgccta
    17161 ttgcttctgt aaggtctata tcctcaattt cttttatttc aagatttttt tcatattgct
    17221 ggaatgctct ctttgttgga atatatccta aatgccaatt tgaattttga gcaattattg
    17281 tattctcaat tcctaaaatt tttttaatat tcatccctat ttctacaaaa atttctccac
    17341 aaataccaat aagtatggtt ttatctccaa ttttaacacc atttaagtca actttaatac
    17401 ttttattttt ccttttctct ttatatatac ttactattcc tgaagcaatc atatatttaa
    17461 ggaaagtttt tttctccttt tttaaaattt cttttgcctt tctatatacc ttttcagtaa
    17521 tttctctata atctaagttc attggaaaat aaaatggctg tatcttttta atttctgttg
    17581 ctttcacatc tattttttct tttaccttct cgcttatttt ttttgctatc tctttcccaa
    17641 tgtttgtact tcttgtttta taatcaaatg gatttatatg atttatatca cctgatggac
    17701 catttaatac aataacttca atttctccaa attccttttt taattcatcc cttaaaaaac
    17761 caggccaatc agaagatatt aaattatcac cgagtgtatc aggatgaaga gcaaaattta
    17821 caataattcc ttttattttc ccgctttcat tgtcgtctat ctttaaaaat gtcaatgttt
    17881 tatcaacttc tcctgctggt tttactatat tttcaatttg attgaaaggg tttgtgataa
    17941 ctcttccatc tttcattaaa tatcttctat taaaagatat tccatcaact tcatttttac
    18001 caactgctat ttttgcctcc tgccttcttc ttaaagcaat ttttacactt gaagcaatat
    18061 aaaatgggag aacttcaaga taactttttt caatataaaa tccttttgta taaattgatt
    18121 cttccgtaaa atcaacaaca ggacctgtat gagtatgtgt tgcatgaatt aaaacattgc
    18181 ctttttttat tccattttct ttcaataaaa tttctcttac tttttttgtt atattatctg
    18241 ataaattaca taaatcacaa ctaattacaa caaaaatatc atcattatca tcaaaaacaa
    18301 cacaacttgc atataggtca tctcttatat caacactcct tctatcctga aaataacctt
    18361 gaagagaaga accaacagga ggtgtaatta caacctttcc aaatccaact ttcatttttc
    18421 ctcctttgta aattcactta atttctgaca aatttacttt tctgttttct ttttcactta
    18481 caagagatgc ctggataaat ttcataattt caagtgtttc ttcttctgcc acaggtgatt
    18541 taccagtttg aaaaaatttc ataatttctt ttaacaattg tgaataaaga ggaacttctt
    18601 ttgaataaat aacctgatat acattttttt cttctaggac agttgctcca tattcatatg
    18661 ctccttttct taatcccctc attgaaccta ttcttccatc attccacttt cctacaacaa
    18721 aatgaaaatc atttgttact tcacaaatta cctgtttaca tccaatcccc ataaatgtgt
    18781 aaagaatttc aactccatga ataccatacc agaataatcc aggattagtt gggtcaagtg
    18841 aagcaggtga aaatgcatca catccaaaaa cattcccgac ttgtttcttt acattctgaa
    18901 tattataatc ataccttaat gatgatgatg aaaaaacagg acagttaaac ttatttgcat
    18961 atttaaaaat ttcttctgca tctttataat ttgctgccat tggtttatca ataaaaagtg
    19021 gctttcttgc ctctataact ggctttgcct cttttaaatg tcttcttcca tcaacacttt
    19081 caagaagtac tgcatcaacc ttatcaagca attcttcaat tgaattgact atttcaactc
    19141 cccatttctc aactaattct tttttaaagc catcaactct tgaataactt gcttcaaggt
    19201 cgggactgaa agaaggataa ccacaaacaa cttttcctcc ctttacataa taagggtcat
    19261 ttttatcact caaaagtttc gtaaaagcag tgacatgcga agtatcaaaa ccaataattc
    19321 caattctaat ttctttttct tccatttttc tcctccattt taataatttc ttttatcaat
    19381 ttatttataa tttcttcttt tttatatgtc ccaattataa tacccttttt aaaaaaagca
    19441 aatttcccaa cacctcctgc tattccaata tctgcctgtt ttgcttctcc tgggccattt
    19501 acactgcatc ccataactgc aatttttaaa tttttaacaa agggatattt tttattcaat
    19561 ctcaaaatct ctttttcaac tttttttaaa acatcaaaaa gggctacctg aattctacca
    19621 catgtcggac aggaaataat ttctggctca aaccttctta accccaatgc ctgtaaaatt
    19681 gattgagcaa tttttatttc ttttacagaa ctttctgtta atgaaacacg aattgtgtca
    19741 ccaatacctt catttagaag aataccaata ccaagaattg atttactttc tgccatgtac
    19801 cctttacctg cttctgttat tcctaaatgt aagggataat taaatttttc tgccaatttt
    19861 ctataaattt ctattgtttc ttttacaaac ggggtttttg cagaaataat aatatcatta
    19921 aaatcattat cctgaaaaaa ttttgtggtt tctgttgtct tttcaattac attttctaca
    19981 agagatcttt taactttaat tgaacccgag tttattccta ttcttattgg gatattttta
    20041 ttttttgcct gttttataat ctcttttaaa tcatttctct tcatattccc aggatttatt
    20101 cttattttat caatcccttt ttcaattgaa taaagagcaa gtttcttatt ataatgaata
    20161 tccgcaacaa ttggaacttt tatttctttt tttagaacct ttattgagtc agtatccttt
    20221 tcattaagaa tagatattct gataatctct gctccctcat tttcaagttt tttgctttct
    20281 tttaataaaa aggaaatatt tgaagttgga acttttgtca tcccctgaat aataactgga
    20341 ctatctccac caacataaat attaccaatt tttatttttc ttgtgttttt tcttatcatt
    20401 ttttaattag agaactgata actcttaaaa tatcattgta tgtaacatat aaagcaagag
    20461 ttattaaaag aattactcct acatactgga caatttcaag aaatcttctt tttgctctaa
    20521 acccagtaat tttttcaaaa aaaagaccaa caagatgccc accatcaaaa aggggaattg
    20581 gaaaaagatt tataattccc aggtttatac ttataaaagc aataaaataa atatattttg
    20641 aaattccata gtgaacaatt tctgctgacc attgtgctat accaactggt cctgataaaa
    20701 gcttcgttga aacttctttt ttaattaaca tcttaaaagc aagaaaaaca ctataagaca
    20761 ttttataaaa ttcttttcct gctcttatca ctgcctgctg aaaaggatat ttttcttcta
    20821 tttcttttgg taatatccct ataacatata ctttttcttt ttctccagta agcgtatctt
    20881 ctgtgtaaga tactggttta attttaaatt gtaatatttt gttgtctctt tttactttta
    20941 attcaatttc tttttccggg tttttttttg cttctttttt tataactgtt aaaacatcaa
    21001 accattcctt acattttttc ccattaattt caactatttc atctccaact ttcagtccca
    21061 ctttttctgc tggcatattt tcaacaaatc cacctatttt agttccatca taaccaggta
    21121 agccaagcat aaaagatggt ataagaagta aaaaaccaag aattaaatta tttaaagaac
    21181 ctcccactaa aattctactt cttactcctg gtggttttcc catgtattcc catttttcaa
    21241 attctccttt atctaaatat tcttctcctg ccattttaac atatccacca aagggaatca
    21301 ggcatatctg ataaagggtc tctccccttt tccatgagaa aagttttgga ccaaaaccaa
    21361 gtgaaaatgt ttcaattttt actcctgcac tttttgcaaa taagaaatgt ccaccttcgt
    21421 gaacaaaaat tataactgaa aataaaaaaa tgactgctat tattgagata atcattttag
    21481 atatttccca tttagatata atttaaaagt ttattttctc accatatttg ccaattaaag
    21541 gaagacaatc tgtttttccc tgtaaagaat taacaatccc tattatcata agaacaaaaa
    21601 caaacatata aacaagataa gaaaggaaat aaaaaattgt ccttaaaaat gggataaaca
    21661 taaaaataaa gtgaataaac caccagccaa tagaaattat caaaagaata agtccctgtt
    21721 ttatatgaaa ttttgtgtaa ggattatctt tttgaataag aactggaatt aaaataagta
    21781 tccctaaata tgaaagccat gcaattgact taccttcttg aatattttgt tgttccattt
    21841 tcctcctttt ggaaaatttt actttttctt taatatcctg tcaaatttat taaaatatac
    21901 cttagggaaa aattacgaga ttttaataaa tatatgtaaa attctgtcat tttaaacttg
    21961 tttttttatt tacataaatt gaggaaaaat ttgttaacat ttcttattta ataatgtggt
    22021 ataataaact ctgcgatatt taaaatggaa aaagaaatat atataattgg agcaggacca
    22081 gcaggtatta cagcaggaat ttatgcttta agaagcaaaa taaaaacaac aataatagaa
    22141 aaattttctc ctggtggaaa tatgttaatt acagagacaa tagagaacta tcctggtttt
    22201 ccagatggta taaatggact tgaactcgca gaaaggatga aaaaacaatt tttaaattta
    22261 ggaggagaaa taattacaaa agaagtagtt gatattgact ttaaaaaaga cagaaaaaca
    22321 atttttcttt cagatggtaa atttattgat acagatgtat ttataattgc aacagggagt
    22381 tcaagaaaaa aattaggggt aaaaggagaa gatgttttta taggtaaggg tgtttcctat
    22441 tgtgctgtat gtgatggatt tttctttaaa aacaaaaata tcgctgttat tggtggaggg
    22501 gattctgcct taaaagaagc aatttatctt acaagatttg ttaataggtg ttatttagtt
    22561 cacagaagag aacagttcag aggttcaaaa tatcttcaag aactgattga aaataatgaa
    22621 aaaatttccc caattgttcc ttttgttgtt gaagaaatta taggtaaaga aaaagttgaa
    22681 tatctcttat taagaaatac aaaaacagat gaaaaaatgg aattaaaagt tgatggtatt
    22741 tttatttcag ttgggcagaa accaaatgtt gaatttttaa aaggaaaact taaatcaaat
    22801 ccatctggat atttaatagt aaatgaaaaa atggagacat ctacaaaagg tgtttttgcc
    22861 tgtggtgatg ttataaagaa ggatttatat caaataataa ctgcctgtgg agaaggagca
    22921 atcgctgcct tttttgctga aaaatatctt gattctcttg attaaatatc taaaaagttt
    22981 tttattagtt ttttcccttc ttctgttaaa attgattcag gatgaaattg cactccataa
    23041 attttataat ttttatgcct tattcccata atttcttcat cttcactcca tgcatcaatt
    23101 tctaaaacag aaggaattga ctttttttct attataagtg aatggtatct tgttgcttga
    23161 aatggatttt tcaaattttt ataaatgttt ttgcctttat gatatatctg tgatgttttt
    23221 ccatgcatca atttttgtgc tccaataatt ttcccaccaa agacataccc aatacattgg
    23281 tgtcctaaac aaacgcctaa aattggaatt tgtccggcaa atttttcaat tatttcacag
    23341 gaaactcctg cattttcagg tctgcccggt ccaggagata taattattct tgaaggtgca
    23401 attttcttta tttcttctat ttttatttta tcatttctat aaacaactat cttttcgcct
    23461 aattctccaa tatattgata aagattgtac acaaatgaat cataattatc tattaaaaga
    23521 accattatat ttcccctgaa atctcatacg cctccattgg acctttactt ttatttattg
    23581 tttcataata ttctcttgta ggtattgaat cagcaacaat tcctgcacct gcttgaatat
    23641 atgctttttc ttttgaaata acaatagttc ttattgtaat acaaaaatcc atatttttct
    23701 gaaaactaaa atatccaact gctcctgcat aaggtcctct tcttgttggc tcaatttctt
    23761 ctattatctc cattgccctt attttaggtg cccctgttac tgttccagca ggaaaagttg
    23821 atattaaaag gtctatcgga ctcttacctt tttttataat tccttcaata tttgaaacaa
    23881 gatgcataac atgagaatat ttttctattt tcataaagtc agtaacttta acactacctg
    23941 gaatacagac ctttccaagg tcatttcttc ctaaatcaac aagcattata tgttctgcct
    24001 tttctttttc atcatttatt agttgatttt caagtttttt atcttctttt tcatctttac
    24061 ccctttttct tgttcctgca attggtctta aaattgcttt tgttccttcc tgtcttacaa
    24121 gaatttccgg agaagaacca attaaatttg tatcctccat ctgtaaaaag aacatatatg
    24181 tagaaggatt aatcatcctt aaacttctat aaatatttaa tggttgtgta tccaattttt
    24241 tttcccacct ttgagaagga acacattgaa ttatatctcc attttttata tattttttta
    24301 ttttttttac actttctaaa aattgcttct gtgaaaaatt gcttttaaat tcttttgtta
    24361 tttgcttttt ctttaatggt tcaaccatat aaagtggctt ttctaatttt actttcatct
    24421 tttctaattt ttcaattccc tcatcatata aactttttaa tgatttattt tctttttcaa
    24481 ttgataaatt tgtaataagt aaaattcttc ttttgaaatg gtcaaaaact atgatatctt
    24541 ttgaaaggat aaatatgaaa tcaggaactt tcaggtcatc ataagaaagt gagggcaatt
    24601 tttctattgt tcttataaag tcataagaaa taaaaccaat aaaaccacct gtaaaaggag
    24661 gaagttgtgg taaaattggt gttttaaatt ttttaagata ttcatcaatt attttaagtg
    24721 gttggttgtt atataatttt ttgctttctc cggttgtatt gtcataaatt tcaatctcat
    24781 tatttttccc ttttataatt aattctggac ccgtccctat aaatgaatac cttcctgtcc
    24841 tttcttcctg ttcagcactt tcaagaagaa aattataaac accacttttt attttcataa
    24901 atgcagagac aggtgtttca atatcaccaa ttatttctct acaaatagga attatattat
    24961 attttttgct tagatatgaa aaaacattgt aatcaggata tattttcatt ttatataagg
    25021 tccgccctct tttttaggac ggacctccag caagaaaatt attcctcaat aattattgct
    25081 tcaactggac aactctctgc tgcttcttta actttttctt ctaaatcaga tgaaatttct
    25141 tttattttta catttgaaac atcgtcttcc atctcaaaaa tctctggaca aatatccaca
    25201 caaagaccac aaccagtgca taaactttca tcaattttta ctctcacttt tgcctccttt
    25261 ttttgcaaaa ttataataat attgttttaa tttgtcaaca aaaaagataa aatatgttaa
    25321 aataaaattc aatttttgac aaggaggtat tatgagcaaa gtagttatta tttattcttc
    25381 taaaacaggt aatacaaaaa aaatggcaga aagtatttat gatggagtaa aagaaataat
    25441 acaaaatgtt gaattaaaat caataacaga tataaaagtt gatgacctgc ttactgctga
    25501 tggaataatt gttggttctc ctacatattt tgggcttgtg tgttcagaaa taaaaaagtt
    25561 ctttgatgaa agtgtagtgc attttggaaa acttgaaggt aaagttggcg gtgcttttgc
    25621 ctcttctgca aatattgctg gtggaaatga aacaacaatt ttaggacttt tgcaaatgat
    25681 gttagttcac ggaatggtta ttcaaggaaa tatttcatca ggtcattatg gacctgtatc
    25741 agttggtttt ccagataaaa atgttttaaa tgaatgcagg gcttatggga aaaagatagg
    25801 aaagttagta aagaaattat ttgataaata atttacataa aggaaaaaaa atggattatt
    25861 caaaaacagt ttacttacca aggactgatt tcccaatgaa agcaaattta agtgaaagag
    25921 aaccacattt tttaaaaata tgggaagaaa aggaaattta caaaaaaata attaaaaaaa
    25981 atgaaaatgg agaaaaatat attcttcatg atggtcctcc ttatgcaaat ggacatattc
    26041 atttagggac tgctttaaat aaaatcttga aggatatagt aataaaatat aaatcattaa
    26101 aaggatatta ttctccatat aaacctggct gggattgtca tggtatgcct attgaacatc
    26161 aaatttttac acaattaaaa ttaaagaaaa atcaagtaga taaaattgat tttagaaaaa
    26221 aagcaggtgt ttttgcaaaa caatttgtaa atatccagag agaagaattt aagcgacttg
    26281 gagttcttgg tgaatgggaa gaaccatatt taacacttga taaggagtat gaaggagata
    26341 ttataaaaac atttggacaa ctatctcttt ctggttatat ttatcaaaca tataaaccaa
    26401 ttttctggtg tataaattgt gaaacagcat tagcagaagc agaaattgaa tattatgata
    26461 taaaatcacc ttcaatatat gtgaagttta aggttattca ggataataaa aataatttca
    26521 gagataatac atattttctt atctggacaa ccactccatg gactttacct tcaaatacag
    26581 gtattgctct ttcacctaca cttaattatg ctattgtcca aacagaaaaa gaggacttaa
    26641 ttataggaga aaagtttgtt tcccaattac ttgagaaaaa aggcattaaa gggaatatta
    26701 aaaaagttgt taaagggact gatttagaag gagttttata tgaaggacct attataaaaa
    26761 gaaaatcaaa agtaataatg gcttcctttg tttcagacca ggaaggaact ggctgtgttc
    26821 atattgcacc cgggcatgga gaagaggact ataatgcagg gattaaaaat ggacttgaaa
    26881 ttttatctcc tgttgatgaa aaaggaactt ttacagatga ggtaaaggaa tttgctggta
    26941 aagttgtttt tgatgcagat aaattaataa tagcaaaact ccagaaagaa ggaaatttat
    27001 ttttagaaga agagattgtt cattcttatc ctcattgctg gagatgtaaa aagccagtaa
    27061 tttatagaag cacaaaacag tggttcctta aagtggacca caaaaattta agggaattgt
    27121 taaaaaaaga aattgaaaaa gtaaattggt ttcctccaga agggaaaaac agaatatatt
    27181 caatggttga attaaggcct gattggtgtt tatcaagaca acgaatatgg ggagttccaa
    27241 ttccagtttt ttattgtaaa aattgtaaaa aaccaattat cacagaagag acaataaaag
    27301 aaattgaaca acaaataaga gaaaatggtt cagatatatg gtttgaaaaa gaagaaaaac
    27361 aactcttacc ggaaaatttc ctctgtcctt attgtggagg taatgagttt gagaaagaaa
    27421 aagatattct tgatgtatgg tttgattcag gagtaagtaa tatatctgtc ttgaaagaaa
    27481 aatatggact taaatggcca gcagaccttt atttagaagg cagtgaccaa catagaggat
    27541 ggtttcagac atctttaatt gtctcctgtg gaacaaaagg gaaagcacct tataaagatg
    27601 ttgttacaca tggatttgtt gttgatgcag aaggcaaaaa aatgagtaaa tcaattggaa
    27661 atgttataac accagaccag ataattaaaa aatatggagc agagatatta agattatggg
    27721 cagtaagtga gaactatcaa aaagatatta gaatatcaga agaaattata aaaaatcttt
    27781 caaaaacata tagaaatata agaaatacaa taagattttt acttggtaat ttatatgatt
    27841 ttgaaattga agagaaaatt gagtatgaca atctttttga agttgataga tgggcaatca
    27901 acaaacttaa agaagttgtc agaaaagttg ataattatta caattcattt aatttcaata
    27961 agggatttga agaaataaat aacttctgta atatctctct ttcttctttt tatcttgact
    28021 atcttaaaga ccgactttac acttatggta aaaattcaaa agagagaaaa acagcacaac
    28081 aggttctttt agaaattctc tcatctcttc ttatgattat atcacctgtt ctttcattta
    28141 catcagaaga agcatatcaa tttttaaaat ggaaagacaa agagagtatt ttccttaaca
    28201 cctggccaga tttaggggaa ccggataaaa aattgcttga tagatgggaa caattttttc
    28261 aggtaaggaa agatattttg aaaaaaatag aagaaaaaag agaagaaaaa ttgataggta
    28321 gcagtttaga agttaaagtt acaataaaaa ctgaaaaaga acaaattgag tttttaaaaa
    28381 gtttccagaa tatagaaaaa cttttaatgg tttctcaagt taattttata gaagggaaag
    28441 aagaaataat tgttgaaaag acagattata aaaaatgtca gagatgctgg ggatattttc
    28501 cagaagtagg gagaaatact gaatttcctg acttatgtga taagtgtata aaagtaataa
    28561 aagagaatgt taacagatta gaaagtagtg agtagagagt ataaacttaa aatttaaagt
    28621 gctaaattta aaactacaac ttaaaattta aaacgaaaca attttaaatt tttttctatt
    28681 gtcagttttg aattttacat tttcgttttg cacttttcgt tttacatttt acacttttaa
    28741 tccgctacaa taccagtgga cgctgaaata ctcgctttgt gcatatgtgc ataaattata
    28801 aacagcactc tgggaagttt aaaaaatgaa atacttaatt gatggttata atgttattaa
    28861 aagttcatat ttagggaaag aaggggactt aaaggggaaa ttgtcacttt atcaaatcct
    28921 tgataattat aaagcaaacc atctatctgt aattttttct gttgtattgg atggttttaa
    28981 tacatctgat ttcccttttt taagagataa aagaataaaa acattctttt ctggtgagat
    29041 aacagcagat gaatatatta aaaaaatact tgaaaaagga aaaagaaatg aagaaataac
    29101 agttgtttca gatgataaac aggttcaaat atcatctaaa ctttttggat gtaatgttat
    29161 gtcagttgat acatttctga atactgtaaa tccaaaagga aataataagc caaaaggtcc
    29221 aatagaaaaa aaaataagtt atagtaaaaa aaagaaaata gaggaggaat taaaaaacta
    29281 ttatgaacta cgaaaaaata cttaatttga aaaaattgga aaatgaattt agggattttt
    29341 ttgaacaaca ggataatgtg agagaagaaa ctttgaaact ggtaagagaa ataggaagaa
    29401 gtgcatttaa atcagttaaa gaaatccatc agggaaaaat aaaagaggca gaagaagaaa
    29461 tagaaaatgg acttaaaatc cttaaaaaag caaaaaaatt attatctaat ttcccagaaa
    29521 tatattatgc tggttttctt cactctgctg aaaaagaaat aatagaagca cttataactt
    29581 tatatctcat aagagacaaa aaaattcctg aatttaacaa agatgatttt gatgtaaaaa
    29641 gttttttaca tggtttatgc gaaagtacag gagaaataag aagatatata cttgataaaa
    29701 taagaagtgg agaaattgaa gaaattgaat tatatcttga aataattgat gaaatttatt
    29761 attttgtttt ggaatttcaa tatacagatg ctttaacaag aggattaaga agacagattg
    29821 attatatgag aggaattgtt gagagaacga gagcagatgt aacttctgct ataatttctt
    29881 ttcgcaaaga tgaaaagaaa taagttaaaa aagaaagcgg actttttaga ataaagtccg
    29941 ctttcttcat ttcaatttta ctttactgct tctttcaatt cacttcctgg tctgaaaaca
    30001 ggaactttct tggctggaat tttaattgtt gcacctgttt gaggatttct tcctgttctt
    30061 gcttttcttt ttgctacttt aaatgtccca aatcccacca atgtaacaac atctcctttc
    30121 ttcaacgcct ttttaattgc ttctataaca gcatcaacgg ctgcaactgc ttcttttttt
    30181 gagcaaacaa cttttgcgac ttcatctacc aattgtcctt tattcatttt ttctcacctc
    30241 ctttctgttt tttatatatt tataaactat tttaaaaatt tgtcaagagg aatttcaaat
    30301 atgataaagg atttaaaatt atttgtcaaa aattttcttt actatcaagg aattccgact
    30361 tttaaaattt gcaatttccc gaacagaata attttcctaa tgggagaaag aggcctgggc
    30421 cggatttgaa ccggcgaata gcgattttgc agaccgctgc cttgccactt ggctaccagg
    30481 cctattatat ttctggaaat ataacttctc ataaaatatt tttacacttc tatataatta
    30541 tgatataaaa ttactttcag gatgtaaaga acaattttaa aattattgga gaaaaaatgt
    30601 ataaggtttt agcaagaaaa tatagacctc ttaattttga tgaatttgtt ggacaggaaa
    30661 atatagtaga aacattaaaa aaagcaattg aaaaaaataa aactgcatat gcttaccttt
    30721 ttgctggacc aagaggaagt ggaaaaacat caatggcaag ggttttcgca aaagcattaa
    30781 attgccaaaa tggtccaact ataaatccat gcaataagtg cgaaatttgt aatgaaattt
    30841 cattaggaac aagtttagat gtaattgaga tagatggtgc ttcaaatagg gggatagatg
    30901 aaataagaac attaagagag aatgtaaatt taagaccatc aaaagcaaga tataaaattt
    30961 atgtaattga tgaagttcat atgttaacaa cagaagcatt taatgcactt ttaaaaacgc
    31021 ttgaagaacc acctgaatat gtaaaattta tatttgcaac aactgctcct gaaaaaatac
    31081 cgccaacaat tatttcaaga tgtcagagat ttgattttaa accacttacg ttgaaagaaa
    31141 tagaaaataa ggttttagaa attagcaaaa aagaaaattt cacaatagaa aaaaaagcca
    31201 tagaagaaat ttctgaattt gctaatggct ctttaagaga tgttttaagt ataatagacc
    31261 agttgattgt tttttcagaa aacaacatta aaattgaaga tgtaagaaaa cttttaggga
    31321 tagttgatga aaaagggata gatgaaattt tgaaactttt agttgaaaaa aaagttaaag
    31381 atgctattgt atttctacat aatttaattt cactgggtaa agacccatct cttataatgg
    31441 aaggtattat taaacgattt agagatattc ttttttacaa aattgaaggt ggagaaaaaa
    31501 aagaaatttt ttcttctttt gataatataa caactgatga attattacag gcaagttcaa
    31561 ttgttattga atttaaagaa aaattaagaa gagaaacaca accgatacta ttatcagaaa
    31621 ttcttctttt taaactttct tctattcttg gaaaagaaat taaaaataat gaaaaccaac
    31681 aggtagatat ttttgaaata acaaagaaag aaaaaaatat aaaagaagaa acaaaagagg
    31741 aaataaaaaa agatacaaaa gagatgataa tagagaagga aagcagagaa aacttaatag
    31801 atgacataat tggaaattgg gatataattt taaaagaaat aaagaacaaa agcagaatat
    31861 tggaagcagc gataagagaa ggaaaagttg aaaaatttga aaatgatact gtttttatct
    31921 cttatgaaaa aaagtttaat tttcacaaaa aaagggctga tgaaaataaa aaagaaattg
    31981 aaaaaataat aagtaaatta acagggaaaa acataaagat tgagacattg ttgaaaaatg
    32041 atacaaaaaa aaatataatt gaagatgaag atgtaaaaaa aatccttgaa gaattcaatg
    32101 gacaaataat tgaaaaggag gaataaaaaa tgtttgataa cttaaaacaa ttagcacaat
    32161 taaaacaaca ggcaaataaa attgaacaac ttatgaggtc taaaatattt gaatcatcat
    32221 ctccaaatga tgaagttaaa ataaaagtta atggaaagat ggaacttata tctattgaaa
    32281 taaaagaaga tattttaaaa aaggaaaata aagcatatat tgaaaaaatt attaaaaaaa
    32341 cattttctca atgtcagaaa gaagttgaaa aatggatggc aacagaagtc gggtctcaat
    32401 taggttcttt taaattacct ttttaataat acaagaaaaa atggttaaaa ttgaaaatgg
    32461 acttataagt ttttgtttaa atgagatttt gtttaaggaa tgtttgatgt ctcttgatgt
    32521 tgatgatggt atccttaaaa gtgtatcttc agaaatttca attaaggata aatctaattt
    32581 tattttgcat tgtaagtttc cagaaaaaaa tattgagtgg gagatatttt ttaaggaaag
    32641 taatggagaa caggttattg aatcaaaaat aatgaattat ggtaagagag cagttaaact
    32701 tggtaaagta tttttattgg atactaaaat tgaaggattt aggaatgaac aagatgaagt
    32761 tgctgttttt ccattacaaa tatatgggca gaaagtttat agaatagatg accccgaaat
    32821 gcctgaaaat agtcaagtaa tagttcaatt ctgcaataat acaaagaatt tatctctcca
    32881 aggtgctttt ctgactttcc aaaggtgcaa taccaaactt gtagttaaaa ggtcagtcca
    32941 tggtcttgat ggattgcaag catattgtga ttttgcagga tgggaactgg caccgggtaa
    33001 atcaacatcc acagagatat ttcgtataat agttggtgat aatccttata cacaacttga
    33061 aaactgggca gataaagtaa aagatataat aaaaccggac atctgggaaa atactccatt
    33121 aggatttaat ggttgtgcct gggctatatt tgatgggaaa gataattatg caaaagttgt
    33181 ttctgaaaat cttaatgcta taaataaacg ccttgctgga tttggattta aatatttatg
    33241 gacaagtatg cataattttg aaggcggtct acctggtaac tggcttaaat ggaattataa
    33301 gcatattcct tgtggtagga aaaaatttat aaagatggtc cgtagtaaag gatttttgcc
    33361 tggtttctgg atagcaccat tttatctctg tagttccctt acggatttat tgaaagagtt
    33421 ggaagatgct attttacaaa atcttgaagg tgaaaaaatg atagtttgtg aagaatggag
    33481 gcacggagat gcaggtaaat tacctaagaa agagcgtcct tgtatttacg ctcttgaccc
    33541 atctcatcca aaaacaataa aatttcttaa aaatgttttt aaaacatata gaaaatgggg
    33601 aataaggtat tatatggtgg attttcttga agcgggagca ggcaatattc atcgttttcc
    33661 ttataaaaaa cattttaata aaaaactcgt agcaggtcca gaggtatata caaatgctct
    33721 taaaatatta aaaaaaactg ctgggaaaga cacatatttg ttaagttcta caggtcctca
    33781 acttcacaat gcaggaatac ttgatggagt tcgtgttggt aatgattttg gagaaggaag
    33841 ggatattaat aaagagtcct ttttttatcc tgcttcctat gtaattaaca aatgtgattt
    33901 ctggacagga cctaaagaag cattgctttc acaggcagcc aattatttca cacaccggaa
    33961 attttacatt gctgactctt caaatcttct tacagtagat aaacctatcc cacttactca
    34021 cgcacaaata catgctacta ttcacgcatt cagtggtgga ccaacaatat taggtgatga
    34081 tattcgtaat attagtgaag aaagattaaa cattattaaa aaaacattac caagaagtaa
    34141 agaaattggt tttcctgtag acctttttac atctgtttca cctgattatc caaaattatt
    34201 tttacgctct attgttaaac catggggtga atataaagtt cttgcaatat acaattttga
    34261 cccggttcct aaaaatataa aagtgccttt tgaaactctt ggaatggaaa aaggacaaag
    34321 ttatttagtg tgggatttct ggaatgaaaa gtttgaaggc aagagaatag ataatattga
    34381 tataaatgtc cgacaagaaa gtgttacagt tttgagattt gtaaaagata aagaacaacc
    34441 agtaatactt ggaacagata tgcatattat gatgggggaa atggaaatta aggattttga
    34501 atataattct aaaaaaatgg aatgctgttt tacagttgaa agacctaaag gggaaagtgg
    34561 aacagtattt atttatgctc ctgaaaacat ttatgttaaa aagacggatg gtttgcatat
    34621 atcaaaagat ggtaattata atacccttgt tatagcagtg ccattgaaat ttaaaaagaa
    34681 ggtagttaag aaaatcataa attttggaca aatggaaaaa gattgcaatt gttgaaaagt
    34741 ttgtgcctaa atataaccaa tgaaatacta tcctgaaatt gttgaaaaac ttattgaaaa
    34801 tttttcaaaa ttacctggta taggacctaa aagtgcagaa agaattgcga attttttaat
    34861 tacaggtgat aatgaattca ttgataattt tgtagaaaac cttgccaacc tgaaaaagaa
    34921 aataaaaata tgtcagaatt gttttaatct ttctgaagat aatatatgta atgtctgtaa
    34981 agatacaaca agagaaaata taatatgtgt tgtagaaaat attaaagacc tggtaatttt
    35041 tgaaaaggca tctttcaaag ggaaatacca tgttttatgg ggaagaattt cctttcttga
    35101 taaaacagac cctgatgact taagaattcc gcaacttatt gaaagagttc aaaaagaaaa
    35161 gacaaaagaa gtcataattg caacgagtac aacaaaagaa ggagaagata ctgccgctta
    35221 tatatctgaa atcctcaaga aattgaatat cccgcattca agaattggtt atggcctacc
    35281 tgttggggca gaaattgaat atgttgacct tcaaacaata aaaaaatcta ttgaaggaag
    35341 aagaggacta taatgttttg tttaatttac aaattttgtt gttttttagg tattcatttg
    35401 cctttaaaag ttgcttattt cattgctgaa caaatcgcaa gattaagata tatttttttt
    35461 ccctctttaa gaaaaactgt cagaaataat ataaaaaaaa cacttgcata tagaaaaaaa
    35521 aagtttggaa ttgattataa aagttatgat gttataaaaa taaccaaaaa agcatatttg
    35581 aattttgcta aatatatgac tgattttttt aatataccaa aatgggatat agaagttgtt
    35641 aaaaataagg tttttttaga aaatttcaat ctacttgata aatgtcttga aaaaggtaaa
    35701 ggagttattg ctttaactgc tcatattgga aactgggaac ttgctggtat tgttgcttca
    35761 attcttggat ataaagtcag tgctattgct attccttatc tatccccttc tgtaacaaaa
    35821 atttatgttg aaagaagaaa agataaagga gtggatgtaa tacttacagg aagtaatcca
    35881 aaacatataa taaaagcatt aagagaaaac agggttcttg caatacttgg ggatagacca
    35941 tttacggaaa agggaatata tgtgaaattc atgggagaaa atgtattatt cccgagaggt
    36001 cctgcaactt tatttattaa aacaaaagcc gaatacattg caggtttttt aattatggaa
    36061 gggaaaaatt atagaatgtt tttcagggaa atagaaaagc caccttcaaa tttgacagaa
    36121 gaagagaaaa ttaaattttt aactcaaaaa ggggcaaata taattgaaga acttattctt
    36181 gcctacccat ctcaatggtt aaacttttca aatgtctggg ataattccgc ctaactattg
    36241 acactcaaaa aacacaaagg tataacaagg aaaaatgtgg aggttaccag tttaatgaaa
    36301 ggatatatct cacaagaaga gattgtgagg gtaagtgaaa aagttgatat tgttgaaatt
    36361 atttcaagat atgtaaatct taaaaaaagt ggtaagaatt atagaggtat ttgtccattc
    36421 catacagaaa aaaccccatc tttttttgta aatcctgaaa aacaaatttt ccattgtttt
    36481 ggatgtggaa taggtggtga tgtttttaag tttataatga atattgaaaa tatttctttc
    36541 ccggaatctg ttgtcaaaat tgcaaaagaa gttggtatag aaataaattt tcaaacaaaa
    36601 caaatttcaa ataaagataa acaggaaata gtaaaagcaa atgagtttgc tttaaaaatt
    36661 tattctgagg tacttttttc ttcccaggga aaacaggcat ctgaatatct gtacaataga
    36721 aattttgatg atatagcaat aaaaaatttt tctcttggtt atgctcctta tgatagaaat
    36781 tttctcttaa aaaaaataaa tgaacattct ctggcaaaag atgttttcat aaaaagtggt
    36841 cttatttcag agaatggaga aaaagatgta ttcaccaata gaataatatt tcctataata
    36901 aacaccaata atgatatagt tggttttggt ggtagaacaa tagaagaaaa tggtctgcca
    36961 aaatatttaa atacaaaaga aaatataatt tttaataaaa gcagaactct ctatggaata
    37021 aactgggcaa aagaaacaat aaaagagaat aactatgcaa ttatcgttga aggatatttt
    37081 gatgttctaa aactattgag taataacata aaaaatgttg ttgcacctat gggaacatct
    37141 ttaacagaag gacacttaaa tatattaaaa aggttcactg ataaaatttt acttgttttt
    37201 gattctgata ctgctggaat aaatgcatct attagaggac ttgaaaatat tctgaaaagc
    37261 ggatttgaag taaagttatg ccctcttcca actggttttg accctgataa atttatagat
    37321 gaatttggta caagttcatt tataaaattg ttaaataact ctcaaaactt tattgatttt
    37381 atgattacca atgaaagtaa aaattttgat ataaattctc caagaggaaa atccattatt
    37441 ataaaagaaa taagtaaatt attatctgtt atacctgatg aaattgaaaa agaagaatat
    37501 ataaaatatc tatcatcaaa aacatctatc ccacaagaaa caattgaaaa atatgtagaa
    37561 gaaaacagcc aatcaaaaga agaaataaaa ccagtgcaaa aaaaacctct taaaccaaat
    37621 gcggaaattt atcttcttga tattatttta aacgaccaga catatttaga acaaataatt
    37681 gagaaaaaag agaaattgac agaaaaatta aaaaaagttg ttgaagcagt tgaaatattg
    37741 ataaacaaaa acataaaacc aactgttgca aatataatag gaataattga tgatgagcag
    37801 attttaaatc ttatttcaaa tgttgcaatt gaagataatc ttatctcaga agataaaaaa
    37861 agaaaaattt ttgatgattg cttaaagaaa gtagaagaaa tatctttaaa ggaaaccctc
    37921 aataagagaa aaaaagaaat atttgaaaag ggtccttctc cttcaaaaaa tgaattagaa
    37981 gaaatacaaa caataatata tcaattgcaa aaagggaggt aaaatgggaa gaaataagaa
    38041 gaatctttct aaaggagaac aaaagaaaaa aatttctgag ataattgaat taggaagaaa
    38101 gaaaaaacat ctctcattag aagaaataaa tgatatccta ccaattgata taacagacca
    38161 gtcagaaata gataatcttt ttgatgaatt agaccactgg gatattgata ttgatgaaaa
    38221 agaacctttt tctattgaga aagaaaaaat aattgatact gaggaagcaa aaaatgcttt
    38281 aaggtcatat atgaaaattg ctggtagtta ttctcttcta actcatgaaa aagaggttga
    38341 gcttgcaaaa ggtattgaag aaatgaagaa aaaaataaat caaatgaaaa aaagaaaaaa
    38401 aatctcccag gttcaataca tgaattgttg tcaacaacta aatgaattaa ggggactctt
    38461 gataaaatca aatcttcgtc ttgttataaa tattgcaaaa agatataaca atcctaaact
    38521 ttcaattctt gacctaattc aagaaggcaa tattggactt atgaaagcag ttgaaaaatt
    38581 caaatataaa actggcttta aattcagtac ttatgcaaca tggtggataa gacaggcaat
    38641 aacaagagca attgccgacc attcatctac tataagaatt cctgtccata tgattgaaaa
    38701 aataaataaa ctgaaaaaat tagaagcaaa atctaatcaa ggaaataatg gtgaattaga
    38761 agatgaagaa attgcaaaaa aactgaaaat acctgttggg aaaataaaaa atataagaag
    38821 gtctatgaga ccagacccta tatctattga tttacctgtt ggagatgaag aaagagcaac
    38881 aattggtgat tttatagaag ataacaagag tcccaatcca ttaaagcgag taaaacaatc
    38941 tcttcttgca gaagaaattg aaaaagcatt aaaggttttg gatgaaaggg aagaaattat
    39001 tataagatta aggtttggtc ttgatgaaaa aagatattca agaacattag aagaagttgg
    39061 agattatttc aatttaacaa gggaaagaat aagacaaata gaatcaaaag caatacaaaa
    39121 attaaaaaat tcaattcatt caaaaaaatt agcattattt cttgaaggaa gttttgaaac
    39181 atcaatacct accaagtaat ataatagaaa tggagaaaga tgtcatttat aaaattcctt
    39241 gggaccgcag gggcaagatt tgtaatgttg aaacaattaa gagcaagtgg tggaatgtgg
    39301 ttttcaataa ataacacaaa ttttattgtt gaccctggtc cctgttgcct ttttagaata
    39361 attaaaagca aaccaaaact gcacccagaa aaattagatg gaatatacct ttctcataaa
    39421 catttagacc attcctgtga aataaattta atgattgagg caatgacaga aggaggtaga
    39481 gaaaagcaag gaattgtttt tactccattg gatgctatct caaatgaccc tgttatatta
    39541 aaatacataa gaaacttccc tgaaaaaata gaaattttac aagagggaaa agagtataat
    39601 ttaaaagata taaaatttga aatacctgta agacataaac atggagtaga aacatatgga
    39661 tttaaattaa aaagcgcaaa tatacctgtt atttcttata tcccggatac tgcttttttc
    39721 ccagaaatta tagaaaaata taaaggaagt gatattctca ttataaatac tgttttatat
    39781 gaaaaaaaag aaaatgtttt acacctttct ttacaggaag cagaagatat aatatctcaa
    39841 ataaaacccc aaaaggcaat tattacacat tttggtatga caatgattaa aaataaaata
    39901 tgggaaaaac agggagaaat aagcaaaagg gcaaaaacag atgttataat tgcatatgat
    39961 ggaatgaatc ttgaaattta aactcttata taaaagtatt tcaaaaggga aaaaattgtt
    40021 cttgttaaaa aatatatttt ctgggaaaaa agaattttat tttagattat cctctttgct
    40081 ttcttctgga tttgatataa tttctgctat ttccaatctt gaaaagaata accccttaca
    40141 tcaaaaagag attgtttctt ttataaaaag agagatatca aaagggaaaa atttatcaga
    40201 agcaatgaaa aatttctctc tcttttctaa ttttgaaata aagagtattt ctgctggaat
    40261 taaatcagga aatcttcctg agacactcct ttctctatct gactattttt cttttcttga
    40321 aaatataaga agaaacatta tttctggttt aacttatcca tttatacttt taaatttagc
    40381 aattattatt cctgccatcc ctactctttt cttaaaggga tttttcccat ttttgggtaa
    40441 aattattatt ccttttatta ttatctatgg aagtattttt gtttttggtt atctacctga
    40501 tatttataaa aagagaaatt tgagagaaaa gttagacgaa ataattataa aaatcccctt
    40561 tttaggaaaa atcttagtaa attttcaagt agttatattt ttaaaaacat ttgttatttt
    40621 gtataaagca ggaattgata ttataagttc atttagagat gcaacagagg ttataggaaa
    40681 tgaaacaata aagaaacaat ttgataaaaa ttttgcttta ttaaaagaaa atatacctgt
    40741 ttcagaagca attaaaaata atcaattttt accagatgaa gttaaaggga tgattgaaac
    40801 aggagaaata agcggaaata tggataaaac aattgaaaaa gttatccaat acacagaaga
    40861 agaaacaaaa tttataataa accagatatt aaaagttatt cctgttgtta tttatcttat
    40921 agttgctgct tatgttggtt atataataat ttctttttac tccggatata taaaaacaat
    40981 aaattctttt ctctaacttc tgcttgtcag aactcttaaa caaatctcta atttattttt
    41041 tatatcgctc aggtctctta attctctctg ccataggtgt tctactttag tatcaataat
    41101 caggacttca agaacttcaa tttctttctc aagttttaaa atcctaaatt cccatatttt
    41161 tggacattaa gatattcata ttgtttttac tgtatgtata aactcaaatg gggataaaga
    41221 catattttct tcttataatg ttcatatagg cattaagtat agcatctacc tttgttaaaa
    41281 aaccctgttg ctgtgatgaa tatatgatat aataaactta cttagtgcct aattatggcg
    41341 gaatttggga aaatatcgct ttttgttttt tagaaatttc agtcaaaaat attttaccat
    41401 tttccatctc tactattttg atttttttaa ttcataaaga agttttataa gagtataatc
    41461 tttaaaaata tctttttctc tcataatttt acaaacacct gaatgtagaa taaaagaaag
    41521 gaaacttaaa aattgtctcg cttgtttctt ctttttatcc caataacttt ctccttcata
    41581 tacataaaca ttcttcccta ttttctattc tatataacta tttaaaattt aaatgtcatc
    41641 tgtttcaatt cctgattttt caatagattt tatcagataa gaaaatagtt attaatatta
    41701 caggatttta ggtttataaa ggaagggaaa tatccgaagg atacttcgga tattttcata
    41761 ttacttgacc ataaataggt tatttgtcaa aagttattat gaaattttat aataaattta
    41821 gtatatctca attcataaat ttgaaagtaa aaatttatca gagtataatt ttttaaaatg
    41881 aggctcaata taacattttt ttctgaaaaa gaaattatac tcccaatttc ttacaattat
    41941 attcttcaaa gttttatata caggaattta aatacctttt taagtaattt tcttcacaaa
    42001 gatggttata tatatgaaaa aagaagtttt aaacttttca cattttcaag aataaatggg
    42061 aaattcagaa aagaacaaga aaattttatt ttctcccctc caatttcttt tatactatcc
    42121 tctccaaaag aagaaatttt agaatgtttt gctgagactc ttgtgaaaaa aaacatcttt
    42181 attttaaata ataactttat ttacatagaa ggcgtagaag tccaattcta tcctaatttt
    42241 tcaaaaaaag aatatgaaat aaaaatgcta tcccctgtta ccgtctattc tacattaaaa
    42301 ggagaaaaca ataagaaaaa aacatattat tattcaccag cagaaaagga atttcaatct
    42361 cttattgtag aaaatttgaa gaaaaaatat tttctaattt atcaggaaga aactgaaata
    42421 aataattttg aaataactcc aatagacatt aatacctcaa atctcaaaat tataaattat
    42481 aaagggacaa ttataaaggg atggatgggg aaatttaaaa taaaaggaga aggagaaatt
    42541 ttaaaagttg cttatgattg tggtattggg agtaaaaacc ctcagggatt tggttgtttt
    42601 gaactacttc tattttctga aaaggaagtt agggggtgat tgatattgcc agaatacatg
    42661 atttatctta aaataaaaaa aggaaatgga aaataaaaac aaaaaaacaa ttcctgaata
    42721 tgtaaaaaaa ctcttctggg atgtcagaaa agaagattta gatatagaaa agcactctac
    42781 atttattatc aaaagagtat tggattatgg tgatgttcca tctctgaaat ggctgattag
    42841 aatttatcct gactatctca taaaaaaagt tatccacaat aaaaaaggtc tacatccaaa
    42901 aacaataaat ttctgggaaa aacattatga aaaaaggcgt gttcttaaat ggttagaata
    42961 taatgacctt gaacttaaaa cccctgagga ataaaaaatg atagaggcaa ttaaagaaat
    43021 cggagagaaa attttatcta atacttcccc tgataagttt ttagaaaatt tggttgaagt
    43081 acctaaaaaa ataaatgggg aggaacagta tattgttatt attgatttta atactgatga
    43141 agaaactgta acctttgatt ttgaagaaat gaaagacgat acacctaaaa aatatttatg
    43201 gataggtaat gctttgtcaa ataatcctca agacaggttt acaacgaata atttggaata
    43261 tttaatatca caaacaattc ctaatgttat agaggcaatt tctgatgggg aattaaaaaa
    43321 actcttagaa aaagtgaaaa ataagtttta ttacgatttg ggagaccata gtaaacagaa
    43381 taaaaaatat cagtatatat tggatataga aaaaataaga atttcatcta aaagtataga
    43441 acaaatatta gaagaagtaa ataataatcc taaaaaatta gttaatactg ttaaaaatga
    43501 aatttttgaa tatttgaaaa gagaaaaaaa tcttactaaa aaacaaatag gacttttcaa
    43561 tttaaaaatt gataatcaac tgatatctga tttcccagaa tatagaaaac atttagaaga
    43621 ttctttaata aaaaagttgt tttctaaaac aattagaggg agatgtttcc tttgtcaaaa
    43681 agaaaaagaa gttacaaaag atatgagatt taaatttagt tattatataa cagataaaat
    43741 tagttttgct tcagagttag aggaaaaagg atttttgaaa aattttactc tttgtgaaga
    43801 atgttataaa aaaattcttg ttggggaatc ttttataaaa aataagatga ggacaaaagt
    43861 tggtttaaat ctttatataa ttccacaatt tatttttgga gatatatcat ttcccataaa
    43921 gaaacttgac aaatgggcag aatacataaa acaatctttg aattctgttg taaatcttga
    43981 aggtttaaga aaatttcaag atagattaga agagtatagt gaatttcagc aacataaaaa
    44041 taactttatt ttaaatcttc tgtattatag aaaatatcaa agtgaattta aaattcttaa
    44101 actaatacta gatgttccac ccagcagatt agatttttta aaagaaaagg aaaatgaaat
    44161 atatgaaata gcaaaaaaaa tttttgggga agataacaga tggtatttga atttagaaag
    44221 aatgtattat ctttttccag tgaggataag taaacaagaa ataggggact ataagaaagt
    44281 attagatttc tacgatgcta ttttttcaac aaaatctata tcttacaaat ttttaattaa
    44341 acagtttgtt gacctaatag gtgtttatag atttgggaaa tttgatagtt ataatataaa
    44401 aaagccaaaa gatactgata ctggattaat ttattcggta ttagaggcaa atcttctttt
    44461 gctctattta aagaaactaa aacttttaca aagaggaggt gataatatga ataacataga
    44521 tacttcttta ctttccgaag gtatgaaaaa gtatattgaa gaaatgggat atggagaaga
    44581 acaatctgca ctttttctac ttggatatct gataggagaa attgggaatg cccagtggag
    44641 taaagaaaat ccgaataaac caattcttaa taaacttgtt tatcaaggaa tggatgtaaa
    44701 aaaaatttta agattaacta atgaggtttt tgaaaaatta aaacagtata aaagaaatat
    44761 tctttataat gaacaaattt ttgctgaatg taaaagatta ttggataaaa atctaaacaa
    44821 ctggcaactt tctaatcaag aaaatgtttt ttatattctt tcaggatatg cttataatat
    44881 gttgaagaaa attgatgaaa acaaattaaa agaaaataaa aatgaggagg tagaaaatga
    44941 atgaagaaaa aattaaaaat tcagatatta tttttattta cgatgcaaag ttatgtaatc
    45001 cgaatggtga ccctgatgat gagaataaac cgcgtatgga ttatcaaact agcaggaatc
    45061 ttgtaagtga tgtaaggtta aaaagataca taagggatta tttatatgca cagggatatg
    45121 aagtttttgt gagcaagata gatgatatga cagtcgatgc tacaaaaagg gtagagaaac
    45181 tttttgaaaa ttatgaagaa aatgggaaaa aaggaggagt tgaaattaat aaacttgaag
    45241 aaaaacatat taaatggcta ttacaaagag ttattgatgt cagaatgttt ggagcaacta
    45301 tgcctatcaa ggcaaaaaag ggaatagcaa aaggagcatc taatgctttt acaggaccag
    45361 ttcagtttaa ttggggatat tctttaaaca aagttcaaat tgtagagtca tcaactataa
    45421 cttctatgct tgcaggaaga ggtgaggaat atagcacaat gggtaaggat tggcgagttt
    45481 attattcttt aattgctttt tacgggataa ttagtggaaa acgagcagaa aaaactatgt
    45541 tttcagaaca agatataaag atattggata atgccttatt aaaatcaata ccacaagaag
    45601 caactaccag aagtaaaatt ggacagaaac caagattatt ggtaaggatt gaatatatag
    45661 ataacacaac ttttttaggt gatttaagaa gatatattaa gatagaacct gatgataatt
    45721 tgcgagatat aaacgagttt gaattagaag taaacaagtt agtaaattta gtcacaaata
    45781 ataaaagtaa gattaataaa atatatctct ggcaagatga tgatttaaaa ttaaaagaag
    45841 ggcaaatttt taaagaactt atagaaaatg gctttaaaga taagattata gatttacctc
    45901 attcagagga gtaaataaat ggaatataag atagatagac ttcttatttt tgatataaaa
    45961 ggggcagtag cgcatttccg gaagttttac accaattcat cttcgctttc atattcattt
    46021 cctccaagaa caactattac aggtttaatt gcaggaattt taggtaggga aagagaccaa
    46081 tattatgaag aatttagtac tgaaaaatgt aagatagcaa tctcaataag aacgccagtg
    46141 agaaaaatta tgcaaacagt taattatata cagacaaaaa gtgataaagg tggaataaaa
    46201 ggagtaaatc ttagtgctgg acatacacag atacctttag aaataatatt accagacaga
    46261 ggttttgaag aacttaaata tcggatatac ttttatcaca aagagcaagt ttttgaacaa
    46321 ttaaagaaag tattaaaaga aaaaaaattt gtctatccac cttatttagg gttaagtgaa
    46381 tttattggag aattagagtt tattgactgc cttgaagaaa ctgatattat ggtatatgct
    46441 tctaaaaaag atactacaac agatttagta actgttgtaa atacagaaaa aattcaagag
    46501 gggggtctta tttttgaaga ggattcagga aaaattttac aatacataaa agaacaaatg
    46561 cctcttgaat ttgattcaca aaggaatttt aagaaaggag caaattttct ttacgaaaaa
    46621 aataatagta acattaaatt aaaaattaaa gagaattact ataaaataaa ttataataac
    46681 tctgagaaaa aagagataga agaaaatatt gtttttatgg aaagttaaat gaaattctac
    46741 tcacatatag gcgaaaaggg gggtaaaaaa gagttaaaac accatctgaa aggagttgga
    46801 gaagaaagta ggcatattat atgtgaatta ccaataaaag acaaatgttt tttttcggat
    46861 atatcctatc ttataggtat ttctcatgat tttgggaaat atacatcttt ttttcaggaa
    46921 tatctaataa atcaaaagga aaataaatta tcacagcatt cttttatctc tgcaatattt
    46981 actgcatggc aaattcaaga atatcttgat gtaaataata attttaagag ttattcttat
    47041 attcctttaa ttggatattt tattgttctt catcatcatg gagatttaag gtcaattgga
    47101 gaagatattc cttcttacaa agattttaaa aatccccctt gtttttccta cgcggaacct
    47161 cgcacaagag aaaaattaca aatgacgtat aaacaaatag aagattttaa acgaccaaat
    47221 aggataagta tcatagaaaa tgaatataaa gaaatggtgg gagcaagcga tataaaagat
    47281 ttcttaaata catggttaag tatattaaaa aaattaaatg aattaaggca aaattatgaa
    47341 aatgaaggag atggaattaa aattgccctt ttcctattta ccttactttt atattcaatt
    47401 cttattgatt ctgataaaag agatgctggg aatgtttcta taataaaaag aaaaaagtta
    47461 atgcctgatt tagttgatag atatagagaa aaacagttta atctttgttc aagaaaaact
    47521 atggatataa tacgaaatca aatttacaag gaatctatat taaaaattga agaagtatct
    47581 cttgaaaatc atctttatac attaacagcc cccacagggt cagggaaaac tttaacttct
    47641 ttctctgtgg cattaaatct tagaactcgt atagaaaaag aaaaaggata tatgccgaga
    47701 attatatatt ctttaccatt tataacaatt atagaacaaa attatgaagt aattcggaat
    47761 gtgctttgtt ctgaaataga agattttaaa gagaatgaaa ctgcttatat tctgaaacat
    47821 cattatttat ccgatttaga gtataaagaa ggagatgaac ttaaaccttt agatgagtcc
    47881 ttacttttga ttgaatcatg gaactcagaa gtagtaatta ctacttttat tcaacttctt
    47941 cacacaataa ttggttttaa aaatagtttt ttaaagaaat atcataatat tgcaggaagt
    48001 ataattcttc ttgacgaagt tcaaaatatt ccaattgaat attgggattt ggttgaaaaa
    48061 gttataaaaa tagttgctga atattttgga tgttacttta ttttacttac cgctacaaaa
    48121 ccattaattt taaaagagga atatgccaca gaaattgtaa aaaatcctaa aacttatttt
    48181 gaaaagttaa accgtgtttt aattaaagca gatttagaaa agaaaaatat agacgatttt
    48241 attaaatggt ttatagaaaa ctataaccaa gaaaattctt atcttatagt agcaaatact
    48301 ataaaatcct caaaagaaat ttatgaaaaa ataaaaactt ataaattaaa gaatccttta
    48361 ttttatttat ctacaagtat tattccgaag cacagagcag aacgaataaa aaagattaaa
    48421 tcacttcttg ataaaaacaa tataccaatt ttagtttcaa cgcaagttgt cgaagcaggg
    48481 gtagatatag attttgatgt agtaataaga gatataggcc cgttggattc aattattcag
    48541 gttgcaggaa gatgtaacag agaatataca aaagataaaa aagaacttta tgttttttct
    48601 cttggacaat ttgcaagtta tgtttatggg aaaattcatc cagaaatttc aagggaatta
    48661 cttaatgcta aagaaatacc cgaaagcaat ttttatgacc taattaacaa ttattttgag
    48721 aaagtaaaat tcaaaataaa tgatgatgtt tcaagatata tttgggaatc tatgcttgaa
    48781 ctgagatttt atgaaaaaaa ccctccagta agaaatggat ttgagaaaat ggtaagtgct
    48841 tttcagttaa ttaaagagaa gggagaatat attgatattt ttgtagaaat agatgaagat
    48901 gcaaaaaata tttgggagag atattgtaaa aaaatatatg aagaaagaga ttttctaatt
    48961 cggcgaaata attatttgaa aattcgtaaa gaatttagaa gttatattat atctattaga
    49021 aattataaag atattttttt accttctgag gtttgtggaa tgagatatat tccaaaaaac
    49081 gaaattaaaa atttttattc acccgaaaca gggttttgtt cagaaataga taccttaata
    49141 ttttaattta tgaacataaa atagtgaaaa tcaatggaat ttaaaataat aggaactttt
    49201 gtttggtact attttatttg taaaagagaa gtgtggttta tggctcataa tattcaacca
    49261 tcacaagaaa atccatatat tgaaattgga agattaatta gtgaagaatt ctacccaaga
    49321 gagaaaaaag aaataaaatt agagtcaatg ataattgata tcataaaatg taaggacgga
    49381 caacttctta ttggagaagt aaagaagagt tcaaaatatt tagaggcagc aaaaatgcaa
    49441 ttgctgtttt atttatatca attaaaagaa aaaggggtag aagcaaaagg ggaattgtta
    49501 attccaaaag agaaaaaaag gattgaagtt gttcttaatg aagaaaatga gagaaaatta
    49561 cttctggata tagaaaaaat aaaaaatata ataagtcaga ataaactacc agaaaaggta
    49621 aaaaatcaat tttgtagaaa ttgtgcttat gatgagttct gttggagtta aaaaatatga
    49681 aaaaaacaat atttgttttt tcagatgggc agtttcagag aaaaggaaat actttatatt
    49741 ttgagacaaa agaaggagag aaaaaatatc ttccagttga aaatataagc gaaataatgg
    49801 taatgggaga agttgattta aataaaaggt ttcttgaatt tttatctcaa acagaaatta
    49861 ttcttcattt tttcaatcac tatggatatt acattggttc attttaccca aggcaacatt
    49921 ataattcagg atatataatt cttaaacagg cagagtttta taatgatgag agaaaaagaa
    49981 tatctcttgc aaaaaaaatt tttaatggag cggtagaaaa tattgaaaag gttttaactt
    50041 actattcaaa tagaggaaaa gaagttggtg ggtatattaa agaaatagaa aacttaaaag
    50101 aaaaggttga taattgtaat tcaatagaag aaataatggc tattgaaggg aacatcagag
    50161 aacattatta ttcttctttt gataaaatcc ttgaagacga agaatttatt tttgagaaaa
    50221 ggagtagaag acccccaaga aataaattaa atacactgat aagttttgga aatagtttat
    50281 tatatgttgc tgttttgagc gaaatttata aaacacatct tgaccccaga attggttttt
    50341 tacatacaac aaattttaga agatttaccc ttaatcttga tattgcagaa atttttaaac
    50401 caataattgt tgatagaata atatttactc ttgttaataa gaaaattatt cagcaaaaac
    50461 attttgagga taaaatgggt gggttaatac taaaagaccc gggaaaacaa atatttgttg
    50521 aggaatttga taatcgtctt agaacaacaa taaaacataa aggacttggg agaaatgttt
    50581 catatagaag gttaatacga atggaactat ataaaataga aaagcatctt ataggagaag
    50641 aagaatataa accatttgtg agtgggtggt aaatgtatgc aatacttgta tatgatataa
    50701 atgaaaaaag agtttcaaaa gcattgaaaa tatgtagaaa atatttatat tgggttcaaa
    50761 attctgtatt tgaaggtgaa ataacatttc caaaacttga aaaattaaaa tcagaattaa
    50821 ataaaaaagt aataaaaagc acagaagatt cagtcattat atatatttta aaagatgaaa
    50881 gatatactaa aagagaaatt cttggcgaaa aaaaagtgaa taagggtatc atactttaaa
    50941 attttttgat ttatcaattt gcaggagttt atatattcgt caatcaccaa tagtgaaaaa
    51001 aatacaggag accgacagaa aaaaaatagt tgacacgaca gaaattttaa tatattataa
    51061 taaatagaac aaaaatgtct ttgaaaagtt ttcttatttg gtgcagagaa gattggtttt
    51121 tagcctacct ataagggatt gaaaccctga tactctgcat aaaatccatc caccgataca
    51181 agtttttagc ctacctataa gggattgaaa ccatattttt gaaattcaca gatagcagaa
    51241 ggtgagcgtt tttagcctac ctataaggga ttgaaacttt gtttatttgt ttaatattct
    51301 cttcagttat gttagttttt agcctaccta taagggattg aaactctac
//
LOCUS       DAOOVW010000006        50852 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_419_length_50852_cov_9.082288, whole genome shotgun sequence.
ACCESSION   DAOOVW010000006 DAOOVW010000000
VERSION     DAOOVW010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 50852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50852
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_419_length_50852_cov_9.082288"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            220..651
                     /locus_tag="PLW95_01470"
     CDS             220..651
                     /locus_tag="PLW95_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOV21338.1"
                     /translation="MKFSTKTRYGVRAMIELALNFNGTPISLKDIAEKQDISEKYLEQ
                     IMLILKKAGLVESIQGTNGGFILVNKPSEIKLSEIVEVLEGGFSPVACVDKEILCKKS
                     KVCSARNVWIKVKNAIKDILDSITLEQLVEEEKKKQSIYYI"
     gene            672..1604
                     /gene="cysK"
                     /locus_tag="PLW95_01475"
     CDS             672..1604
                     /gene="cysK"
                     /locus_tag="PLW95_01475"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOV21339.1"
                     /translation="MKVSKNITNLIGNTPLVKLNKLTEKCYAEIYAKLEFFNPCGSIK
                     DRVAVNMIEEAERKGIINNDTVIIEPTSGNTGIGLAFVCAQKGYKLILTMPENMSTER
                     RKLLSLFGAEVVLTPIREGMSGAIKKAEKISKKYKKSFMPQQFKNPANPDIHRKTTAE
                     EIWKDTDGNIDIFVAGIGTGGTITGVGESLKKRKKSVKIIGIEPENSSVLSGGKPGEH
                     KIEGIGAGFIPDILNIGIIDEIIKVSDKDAIETTEKLAKQEGILAGISSGAGTFASIK
                     IGMRKENKGKLIVVIFPDTGERYLSVLNEKRAEK"
     gene            1616..2392
                     /locus_tag="PLW95_01480"
     CDS             1616..2392
                     /locus_tag="PLW95_01480"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016359031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HOV21340.1"
                     /translation="MEKEISKGIIESYMKEFIDNLEVDVAIVGAGPSGLICGYYLAKK
                     RIKVAIFERSLRVGGGMPGGGMMFNKIVIQKEAKEILDEFEITLKEYENGLYIANSVE
                     TISSFAYRTLKAGAKIFNLISVEDVVIREKRINGVVLNWSAVGIAKLHVDPLVVLSNY
                     VVDATGHDCEVCRIVEKKVGGIEVKGEKSMWAEKGEKEILGNTGQVYPGLMVCGMAAN
                     AFYGSPRMGAIFGGMLLSGKKAAELVEREVLNGKNNRRDK"
     gene            2364..3557
                     /locus_tag="PLW95_01485"
     CDS             2364..3557
                     /locus_tag="PLW95_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HOV21341.1"
                     /translation="MAKTIEEINEKIKKGKVVVVTAEELIDMKKSANSKKLSEEIDVV
                     TTGTFGPMCSSGLLINFGHSKPKIKMGGGSVYLNKVPAYAGLAAVDVYIGAAALPDND
                     PRNSNYPGEFKYGGGHVIEDLVSNKEIELKVETYGTDCYPRKHLKTKLKLNDLNETTL
                     FNPRNCYQNYNVAVNLSDRTIYTYMGILKPNLGNANYSSAGQLSPLLKDPYLKTIGSG
                     TRIFLGGGIGYVAWWGTQFNPSVKRKENGIPIEGGCTLTLIGDLKQMSPDFLKGASLT
                     GYGVSLIVGVGIPIPILNEEILNFASIPDEEIYAPVVDYSNDYPNAIEKVICWVNYAQ
                     LKSGKVEIMGKKVPTGSLSSYKKSREIANILKNWIGSGKFLLTEPIFHFPGPDSGYMP
                     KPMKI"
     gene            3569..3976
                     /locus_tag="PLW95_01490"
     CDS             3569..3976
                     /locus_tag="PLW95_01490"
                     /inference="COORDINATES: protein motif:HMM:NF020940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOV21342.1"
                     /translation="MEIKKRYVMHFPPNLIDKPIISDAVRKYNFQFNILKAYITPEEE
                     GTLVIELIGEEKNLENTENVLKEMGVKVQTIESDIKMLKEKCTDCGVCVPLCPTGALK
                     KEDDFTISFLPSKCVACEICIKACPTKAMISTI"
     gene            3978..4877
                     /gene="nadA"
                     /locus_tag="PLW95_01495"
     CDS             3978..4877
                     /gene="nadA"
                     /locus_tag="PLW95_01495"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOV21343.1"
                     /translation="MDKEIRKLKEEKNAVILAHNYQIPEVQEIADFVGDSFELSKKAS
                     EIKDAEIIVFCGVKFMAETAKILSPSKKVLLPEMDATCPMADMVKVQDVIKMKEKEPD
                     AWVVSYVNTNAEVKCLSDVCCTSGNAEKIVRNIPAKKIIFLPDKNLCWYVQQRVKEKE
                     IICWDGFCYVHQQFTTDDIKKARKKYPEAEIVVHPECDPEVQKLADGIYSTSGMLKRV
                     KESKTKKFIIGTEEGIIYRMKKENPEKEFYPLGDKKVCSDMKKITFESVKRSLEEEIY
                     QINLGKEIIEKGKISLEKMFEYS"
     gene            4942..5331
                     /locus_tag="PLW95_01500"
     CDS             4942..5331
                     /locus_tag="PLW95_01500"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOV21344.1"
                     /translation="MKENGKVIELKGDIAVVKMERKNKCEKCGLCEKIAGRDPFIEVK
                     NKIDAKIGDEVEVEIKEDDLLKISIFIFGFPLLGFILGIISSYFCKNITLKIVVFLVF
                     LLSFWIAGFKKGKNYAENTKPHIVSKI"
     gene            5382..5609
                     /locus_tag="PLW95_01505"
     CDS             5382..5609
                     /locus_tag="PLW95_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOV21345.1"
                     /translation="MELKEKVEKAIEDVREFLQTEGGDCEIVDVENEKVKVRLKGTCR
                     GCPFAVITLKMRIESIIKERVPEIKEVVEVD"
     gene            5596..5892
                     /locus_tag="PLW95_01510"
     CDS             5596..5892
                     /locus_tag="PLW95_01510"
                     /inference="COORDINATES: protein motif:HMM:NF013334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase/urease maturation nickel
                     metallochaperone HypA"
                     /protein_id="HOV21346.1"
                     /translation="MKLTNDIKLKGISMHEFSVVENLIKILKKVIKENNAKKVLKVNL
                     KINPFSCLDQENLNFIFSSIVKNNDFLENTKIIIKRGKDPLSREYIVENVEIEI"
     gene            5917..7059
                     /locus_tag="PLW95_01515"
     CDS             5917..7059
                     /locus_tag="PLW95_01515"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOV21347.1"
                     /translation="MMDIFKIKNAKTKRISSYDVEGRNADRWIINPGEKKVLAEIEGT
                     GCITHIWMTQSGNYSLYRDVLLKFYWDEEENPSILVPLGDFFCLGHSLVNSFCCLPFS
                     ASTNTPYIFGGGCALNCYLPMPFNKGAKIEILNESDQAYGQYFYIDYEIYENNFEGEI
                     GYLHSQWKRENPTPGWGNEFKVNIPEVDIVNKEKIAYNNNYVIMEGEGRGHFIGFNLS
                     VTNFHSDWWGEGDEMVWVDGYKWPPDLHGTGSEDCFNQAWGMQENAFLFNGSSIYEKN
                     SIFGHSLYDGKLQGGYQTSYIFYITNPIHFKKSIKFTIEHGHGNHLSNDYSSTAYWYQ
                     IEPHKSFGILPVEKRKPILLKFENSPQLQTEKRKIILTEEMKKRKK"
     gene            7062..7808
                     /locus_tag="PLW95_01520"
     CDS             7062..7808
                     /locus_tag="PLW95_01520"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOV21348.1"
                     /translation="MLFIIKCLLAGGIAGFLCGLLGIGGGSIIVPVLFFFFSVPIKNA
                     IATSLMVVLISAISGFLVHLKGKNTDFKLAFYLIISGVIGAYIGAYLTGILPEMIVKG
                     FFMVLLIGLGLKLLFQKENEKEDNKEYSLDILKTLSIGFISGIVSGLCGVGGAVLLVP
                     LSYIILKVPIKICVGTSLIVVFFNALSGVFAYAKMGFIDYRIGLTFGIVGIITSPIGA
                     KVSILTPREKLRKIFAIFLILMPILLLLRK"
     gene            7805..8665
                     /locus_tag="PLW95_01525"
     CDS             7805..8665
                     /locus_tag="PLW95_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21349.1"
                     /translation="MKIIGIDENGFGSLTGPLIITGTSIKSEEGKWFASVCDSKKFFS
                     SRKTNNFKKLEEFVISLYYCIKRKLPESSKEVINFFSPDFKCSGMSDICSVNIPDNFI
                     WADIEKAKKQSEKFIEWMEKEDVSIKDVKSKIICAKKFNELIKNGNSKFIIDLFYFCE
                     ILKEMGREDYFVYAGKIGSMRYYSKYLRYFFSNYEIYPEIEEEKKSIYVLNGRRNFKL
                     GFFFNVEEVSFASSISSIVGKYIREIFMESFRKSTGIEEKISGYRDKKTKRVISNLFS
                     FPQECVLRIK"
     gene            complement(8658..11180)
                     /locus_tag="PLW95_01530"
     CDS             complement(8658..11180)
                     /locus_tag="PLW95_01530"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl-hydrolase"
                     /protein_id="HOV21350.1"
                     /translation="MNIKYYGENLKQIKFLLGGIGTGNVSVEGTGSITDWEIFNRPSK
                     SNEMYGNFVALRVEQKRKVFQKIVARKPFPPFEGSHGFKNIRMEGYPHFEECIFTNFF
                     PFAIIDFFDRSIGVSVSLNAWSPFIPGDVENSSLPIVIFSYKIKNLTRDKINIFLSFS
                     MMNPVGTDGTEKLNSYINKCFGQNINNFVETENLKGISFTSKKYNEESERYGNLSLFT
                     DAISVSYKNQWKNEGWWDDLKNFWKEFKIGNFEPVSPKISPDSQTYWASLGVNKNLAP
                     KEETDINFYLSWYFPNRINYWGLDEEVKGKILKNYYSIKFSNSMDVIKYFIKNKRYLE
                     EKSKKFAERFFSQSLPESFLNTTSAQLNTLRSNTFFITSDGKFYGFEGCSDNSGCCPL
                     NCTHVHNYDFTVPFLFPSLSRSQREVDYLYNTDENGYMAFRTNVPLGIKRWLSLPAAD
                     GQMGTILKLYREWKISGDDEFLKKLYPSAKKTIEYAFENWDKNRDGLMEGQQHNTYDI
                     EFYGDNPFTSFIYLAALKVMIEMAEYNSDNNFASFCNEIYQKGQKNIIEKLWNGRYFI
                     QKCSINPVPPYQFLNGCLSMQLLGQFFADTLNLGSLVEEKYINKALFSILKLNFLENF
                     SQHQNYMRIFAIGEEKGLLVCTWKEGEKPEIPMPYCDEVFTGEEFVIACLLLKRGYYK
                     ECLKIINGMDNRYDGIKRNPFNHLECGHHYARGLSGWGLILSYLGFWIDNVKKEIFFN
                     PLLFRKNFSIFFSCGTGWGVYNYKILPGRENFKIEIDEGFIEVRKIYLSYWKKKKTNF
                     VKTIIDGIETNNKFSEESNKIIVEFENIKRVEKSIEISRQFI"
     gene            complement(11194..12795)
                     /gene="argS"
                     /locus_tag="PLW95_01535"
     CDS             complement(11194..12795)
                     /gene="argS"
                     /locus_tag="PLW95_01535"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOV21351.1"
                     /translation="MKKIKKRIEEGIKKVIEEISNKVIDVDVFPSEKENFGDFTTNIV
                     FKLGNDYAEKIREKILPIVSDIVEKIEIKNNYINFFVSKNVYFNFLKELVKAGKDVLK
                     SEIGRGENVLIEFVSANPTGPLTIAHGRQASFGEALSRIMKFANFSVVKEFYINDSGK
                     QIDLLGESLKARYYQLKGEEKYTIPEEGYHGEYLIQLAKEIKEEKVDDEFFKKFAYER
                     ILEEIKRDLRNFGVEFDSWVNESIFIKERKVDKIINELKEKGYIYFKDGSWWFKSSIF
                     GDDKDRVIIKSDGTYTYLATDIAYHKYKIERNFSILINIVGPDHHGYIPRMKAAVQAM
                     GFEPEKLKFLIVQLTTLYKGKEKLSMSTRKGEFISLKQLIDEVGPDASKFFFLFRKVD
                     SHLDFDIELAKKQSTENPVFYLQYAYVRIKHILNFAESKNITIGKTEFDPDYLKEEEE
                     LILLKKMWKFPDIIESVVKTYGVHLLCEYLLNLAKIFHSYYHKYRVVGEDKKTTESRL
                     FLIKGLYTIFSLSLDLLNISLLEEM"
     gene            13272..15362
                     /locus_tag="PLW95_01540"
     CDS             13272..15362
                     /locus_tag="PLW95_01540"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOV21352.1"
                     /translation="MKFKDIERWVKGIIVFFIALYLFLSLISFDPFDPTFKFCQIGSP
                     VNTEISHFGGRVGSYIAGLLIFIFGKGAYLLVFLLLFSAYNVIWGEKINFGKKIISIF
                     GIVFVFSIFLSLQTKNFSGGFLGGVFTPYLKEFFGEKGIYLILILLFVVFFGLVYKMF
                     VSPFQFLFARDVFSTTAEGKEMLRKKPVMKKRVIKKNEQGPEIEEKEVQKEKSEQKVI
                     ITTKQKNFVLPPVKLLKSDTFPQKETKDDFERYAQVIEETLSEFDIEGEVVQINQGPR
                     VTMFEIQLAPGIPVQKIFKIQDNIAMNLKTTSIRIVAPIPNKSTVGVEVPNREISTVY
                     LKELLVSKEFQKSPSKLTIAIGKDIMGRSVVSDLKIMPHLLIAGATGSGKTVCLNSLI
                     TSILYKATPDEVKFILIDPKMVELTLYNGIPHLLCPVVIDIKKAVNVLKWLIQEMERR
                     YKLFSEKRVRNIETYNLQFKEEKIPYVVIVIDELADLMIVAKNEIEQSIIRLAQLSRA
                     AGIHLILATQRPSVNVITGVIKANLPCRISFQVTSKFDSRTILDKIGAEKLLGRGDLL
                     FIPPQSSIPLRIQGSFISDEEIESICNFLKSQSSPQYNMEILEKKLFDETEIEHFSSS
                     SAKLDDDILYQEAKNIVLTTKIASISMLQRRLNIGFNRAAKFVEKMEEEGIVGPYIEG
                     KPRKVLISNENRER"
     gene            15343..15741
                     /locus_tag="PLW95_01545"
     CDS             15343..15741
                     /locus_tag="PLW95_01545"
                     /inference="COORDINATES: protein motif:HMM:NF024805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOV21353.1"
                     /translation="MKIEKDKINVLLKETREKKGIGIDEIIENTRIPQKYIKIIEDGQ
                     WEKFPSQTYLLGYLKIYCEYLNIKKEIIDTILSEFKQENKKEKEVIKEEKKDNVEQKR
                     EKILLIILPLIFFSIYFFTLYILSDISSNL"
     gene            15743..17041
                     /gene="rimO"
                     /locus_tag="PLW95_01550"
     CDS             15743..17041
                     /gene="rimO"
                     /locus_tag="PLW95_01550"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOV21354.1"
                     /translation="MGKKVSIISLGCAKNLVDSENILALLGEDKFVITDSYKESDIVI
                     INTCAFIKDAVDESIRVIKKIAEKKNRKKIIVCGCLVQRFKEKIFKIADIDGAIGTGD
                     PGKVFELVKTLKDNKKILLVDDKTFVTSKTYPRLITTFPYAYLKISEGCNNKCNYCLI
                     PYLRGNQRSRKIKDILKEADDLSKSEIKEIVIIAQDTTNYGTDLRYGTNLKELLVEIT
                     KYDFKWVRIMYCNPARITGDLIEEISKNEKICKYLDIPLQHSHPEIIKKMGRPVINYN
                     KIIENIRKGIKEVRLRTTFMIGFPGEKEVHFKHLVNFIKENKFDRCGFFKYSREKGTS
                     SFDFDQQIDEEIKDKRLEKVVKIQERISKNKLKNMVGKTYDVLIEKKDGQFYIGRTEY
                     DAPEVDGVVFVSGKNLKLGNFYKVKITSSSAYDISGEVII"
     gene            complement(17046..18089)
                     /locus_tag="PLW95_01555"
     CDS             complement(17046..18089)
                     /locus_tag="PLW95_01555"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOV21355.1"
                     /translation="MNKEISLEVNCERIEKVREFLKKKKGGCFLTFNPSNIFYLTGIS
                     EVEGILAITEKKVDLFVPELCFYQAIDSNKNIQIHKYNEDDLINFLDDFKKTFFISTE
                     ISYLTYLKISKRTKRQFVPAENIFISLRSIKDEKEIELIKEAKKITEKTLDEIYNFIQ
                     KENKFTELDLLGEIKYLLNKNGAESESFEPIVASGIHSSYPHHKAKNKTIEKGEVIVV
                     DIGADFCGYKSDLTNTFFYGDVSDEIKNVYKIVKDVREIATEYIYEKKQKGKIRGKDV
                     HQKAITIFKKYNLEKYFIHSLGHGVGIDIHEKPNLAPGSNDIIEKGNVFTIEPGIYMH
                     KKFGIRLEQMVIL"
     gene            complement(18110..19024)
                     /gene="argF"
                     /locus_tag="PLW95_01560"
     CDS             complement(18110..19024)
                     /gene="argF"
                     /locus_tag="PLW95_01560"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOV21356.1"
                     /translation="MGKRDFLSILDLSKEEIFKIFDLSKMYKSKWARREIENILRGRT
                     IGLIFNKPSTRTRTSFEVAICSLGGNTIYLEESNIQISRGETIKDTSNVLSKYLDGLV
                     IRTYSHSEVEEFAKWCNFPVINALTDLLHPCQILSDYFTIFEKFGKLSGLKLTYIGDG
                     NNVCNSLILGGDLLGVEVRVICPDDYMPSEKILKMVKNKIRISNNPYDFIEDTDILYT
                     DTWISMGDEKEKEKRLEIFKKYQVNSKLIAKIKTDFIFMHCLPAHRNWEVTDEIIDGK
                     NSIVYTQAENRLHCQKGLLHFLYSFYKP"
     gene            complement(19031..20218)
                     /locus_tag="PLW95_01565"
     CDS             complement(19031..20218)
                     /locus_tag="PLW95_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOV21357.1"
                     /translation="MEKNQIKQLYEHYVIGCYSREDVVFVRGYGSWLWDINGEKYLDF
                     FPGWAVSGLGHCYPEVVKSITKQAKKLIHLPNNYYHPWQAILAKEIVDISFDGKLFFC
                     NSGAEANEGAIKLSRLYGKEKGKYKIISMENSFHGRTLATVTLTGQKIYNEPFKPLPE
                     GFSYVPFNDFHALKEKIDDKTVSIFIEIIQGEGGINVVDKEYLENVYQLCKEKDVLLV
                     FDEVQTGFGKTGKFFAYQHFGIQPDIMTLAKTLGGGFPIGAMIAKREIADLLVPGTHA
                     STFGGSPLACASAISVIRTIKEKDILERVNKYGKYLKNKLEKLKEKYTCIKKVKGIGL
                     MLAIELEMDGRQFYKLCLNKKLLLNCTHGNVIRIMPSLTVKKKEIDRAVEIMDECFSE
                     MKD"
     gene            complement(20199..21089)
                     /gene="argB"
                     /locus_tag="PLW95_01570"
     CDS             complement(20199..21089)
                     /gene="argB"
                     /locus_tag="PLW95_01570"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOV21358.1"
                     /translation="MDDEILKKADVLLESYPYIKNYKGKIFVIKVGGSILKIEQARKD
                     ILQDIAFLEVVGINVILICGGGPFITEEIEKKGSKVTFVDGLRVTDKNTLQIVQDVLY
                     RVRDGIVDELVEKFQASASSILPEEKYVIAKKIHYQRGAEVVDLGFVGQVDQVNNEYI
                     KNKLEKYKVLVAAPLFYDKDGVLLNVNADSVASSIAESLKAEKLIFLTDVLGIMRHPE
                     KSETLISILELSEGNELIEKKVIKEGMIPKAISSISAIKNGVKKVHIISGNISHSILL
                     EVFTKAGVGTEIIKDGEKPD"
     gene            complement(21179..21946)
                     /gene="purQ"
                     /locus_tag="PLW95_01575"
     CDS             complement(21179..21946)
                     /gene="purQ"
                     /locus_tag="PLW95_01575"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HOV21359.1"
                     /translation="MVKVNVLILRVAGTNCDLETEWAFKLAGGVPERIHINRIIENKK
                     LLEKYQILIIPGGFAYGDDISAGKVLANQIKFKLWDEMQKFIEDKKVIIGICNGFQVL
                     VKSGILPWIGKQSVTLTWNNSSKFEDRWIYLKLENSVCHFFKGLPEIIKLPVAHAEGK
                     FIPENKEILKKINEKKLVLFRYCDKNGEYSSYPYNPNGSVENIAGICNQTGLVLGMMP
                     HPERCVLKYHLPDWTNSSKNKYTYGFKFFKNVVEYFS"
     gene            complement(21940..24303)
                     /gene="purL"
                     /locus_tag="PLW95_01580"
     CDS             complement(21940..24303)
                     /gene="purL"
                     /locus_tag="PLW95_01580"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HOV21360.1"
                     /translation="MKYAKEIEIIGKGDRQLLEISKKGLLSLDIDEMRAIERYFKTIG
                     RNPSDCELETIAQTWSEHCYHKTFKANIKYQEKEGKKKKEKNVVLLKEIMKVTEKLNS
                     PLCISVFKDNAGIIKLDEKYGVAFKVETHNHPSALEPYGGAGTGIGGVIRDILGAGLG
                     AKPILNTDIFCFGNMDTPFEKLSEGVLHPKRIFKGVVSGVRDYGNRMGIPTGNGAIIF
                     DERYTNNCLVYCGTVGLIPIDKVEKRNVENDYIVVVGGKTGRDGIHGATFSSTSLDKD
                     IPTSVVQIGNPIIEKKVLDCLLVARDKDMYNSITDCGAGGLSSAIGEMGEKVGAVVYL
                     DKVPLKYEGLNGWEIWISESQERMVLSVPENKLKELIELFSSEDVDVSVIGKFEKTGK
                     LIIHFRDEIICDIDMKFLHKGLQKKSLESKFEIKKIKEKCKKKIDLKEGILKVISSPV
                     ISSKEEVIRQYDHEVQSRTILKPLIGQNGPSDAVVLKVHYDKNYGIAVGCGINPFYGD
                     IDPYYMAGCCIEETIRNLVCVGTNPEKIFILDNFCWGDIGDKKQLGSLVRCVNGCKDF
                     ALKYKVPFISGKDSLNNYFIDEKRRKISIPGTLLISGLGIIENIDNVISSDLKKEENL
                     IYICGETKREMGGSQFFRTYNFNGGRVPVPEPAKTTKLIKKIYKAINKNLIRACHDCS
                     DGGFSTCISEMAIGGRKGAEIYLSKIPSKDRKIDTYLFSETQGRFVVEIEEKDKKLFE
                     ETMKGSEFSCIGRVNKSNYLKFYSGEKELFSLDIELLSNKWRSGVKW"
     gene            complement(24304..24768)
                     /locus_tag="PLW95_01585"
     CDS             complement(24304..24768)
                     /locus_tag="PLW95_01585"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF014726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HOV21361.1"
                     /translation="MKYIIEIFYKENVFDAFGNETKKGVEEMGIYDVKKVNVSDLYAF
                     DGNVEIKEIKEIAENILLDRVSQKYSINIETTKEKNLYLVEIWYKKGVTDMVAETTKK
                     AISDYGLKKDINISTGKKYYIYCSLKQTEIKKICVRLLANPLIHDYKIKRGN"
     gene            complement(24765..25637)
                     /locus_tag="PLW95_01590"
     CDS             complement(24765..25637)
                     /locus_tag="PLW95_01590"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HOV21362.1"
                     /translation="MKFEIVYSPDFLRYYEKGHPECPERIKVIYNFLIKKGINNFVGV
                     DLYNEDLLFLAHSREFVENVKNNNFSAMDTPVILDIYKYAFLSVCAGVKASEISLETS
                     NIGISLSRPPGHHAGKHFLGGFCYFNGIAVAVKKIINEGKKVAVLDIDGHHGNGTEDI
                     LKMGKNVIYVSIHQFPAFPGTGNFSFENCYNFPIAPGTGNKKYLEKFYNTLNIIKEFS
                     PDIIGISLGFDTHKKDPLLSLNLEESDYYLMGKEIGKTGMNLFIIFEGGYNLDVLGSS
                     FYCFIKGIERGREK"
     gene            complement(25627..26385)
                     /locus_tag="PLW95_01595"
     CDS             complement(25627..26385)
                     /locus_tag="PLW95_01595"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOV21363.1"
                     /translation="MEEIKVRTGYKIENYILEEMLGAGGFSTVYKARSIDPQPEYDPV
                     IAIKVLHPRRLDRYQIRRFIREAKIAKKLIHPNIVKVYDFKKDEGAFFIFMEFLDADL
                     LKCIHTRREIFSPSILEEIILKSAKGLSFIHQNRIIHKDFNPSNILVNFSLEKVKITD
                     FGLSVKNTFFYKEKREGGTLDYTAPEIIKGRKASIRSDIYSFGKTVEKLYNELNFPIP
                     DTFRYIINISTRENPDERFSNMEEIIYMFEDYEI"
     gene            complement(26588..28111)
                     /locus_tag="PLW95_01600"
     CDS             complement(26588..28111)
                     /locus_tag="PLW95_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOV21364.1"
                     /translation="MLSKVRSITIWGIEGFPIEVEVDIARGIPGISVVGLPDQAVKES
                     RDRIKPAIKNSGYEFPNRKIVINLAPADIKKEGSYFDLPISIGILSALGYIPKDTLSN
                     YYIVGELALNGDVRPVKGILPMVLKLKEDGCDKIILPDENKFEGGVVEGVEIYPVKTL
                     NEASSFLKGDTQIKPFKVNFNDLFEEKDKYSVDFKEVKDQKYVKRAVEIAVSGFHNIL
                     MFGSPGAGKSMIASRIPTILPPLTLTESLEITKIHSVAGILKTPIVIERPFRSPHHTI
                     SDIALIGGGTIPKPGEISLSHNGVLFLDELPEFKRNVLEALRQPLENGNVSVSRAKGR
                     VDFPARFLFVAAMNPCPCGWYGDIHHTCNCTLSQILKYRKKISGPLLDRIDIHIEVTT
                     VPADVLIEDREEESSEEIRNRVVKARQIQRERFKNKGIYFNAYMNSQQIKKYCIIEKE
                     GEEILRETMRKVNFSARSYDKIRKVARTIADLDASEIINASHISEAIGYRNTDLDIL"
     gene            complement(28152..28592)
                     /locus_tag="PLW95_01605"
     CDS             complement(28152..28592)
                     /locus_tag="PLW95_01605"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOV21365.1"
                     /translation="MAKLAKWSPFKEIMDVRDDFDRLVDRIFNRDLDLWEGGRVPAID
                     VYEEGDNIIVKAEIPGVKKEDISVSLNGDILTISGKSQEEKEVKKENFYRKEIRSGTF
                     SRSLTLPAQIDKDKVKASYKDGVLQLTLPKSPEEKKKEVKIKVE"
     gene            complement(28646..28879)
                     /locus_tag="PLW95_01610"
     CDS             complement(28646..28879)
                     /locus_tag="PLW95_01610"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOV21366.1"
                     /translation="MKCKDIEKLISEYIDGELEKPINDLIKEHIKICEKCLSLFESLE
                     KTIFLSKKIYKKKSVPKVISKRIYHQIEIKFKK"
     gene            complement(28881..29477)
                     /locus_tag="PLW95_01615"
     CDS             complement(28881..29477)
                     /locus_tag="PLW95_01615"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOV21367.1"
                     /translation="MKFTRNKMSEQQLIERAKKGDTKAFEELMKRTQTNIYNLGFRLL
                     GNKEDAADLMQDTYIKAYENLDKFEGRSSFSTWLYRIATNNALMKLRKEKNKNISIDE
                     IKKIGDKDYKIEISDWYENPSLYFKSNELKQQLQEAIDSLPPKYKSVFILHDIEELPI
                     SEVAKILSLSIPAVKTRIHRSRMFLREKISEYIKKHGN"
     gene            complement(29458..29679)
                     /locus_tag="PLW95_01620"
     CDS             complement(29458..29679)
                     /locus_tag="PLW95_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015126523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HOV21368.1"
                     /translation="MKITIKVKTDAKQDKIEEKEGIFSVCVKERAEKGKANKKLIEIL
                     SKYFNVPKQNIEIITGKTSRNKIVEIYQK"
     gene            29781..30329
                     /locus_tag="PLW95_01625"
     CDS             29781..30329
                     /locus_tag="PLW95_01625"
                     /inference="COORDINATES: protein motif:HMM:NF018701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HOV21369.1"
                     /translation="MKKIIIFLLVVLFAGCAVVNVYVTFPEEKIKKAAEDILAPAPSS
                     PSGFLKYNFTKNLYADEKVVVGKELKTDSPVIRQAQEKINSWRSKLDEFKKDGYVGET
                     NDFKVVIKQVPDDKQKETELRELVKNENTQRDIIISELMKINNAPAKEEKKFREIFAE
                     TSIKYSPSGTWIQDAKGNWKRK"
     gene            30326..31366
                     /locus_tag="PLW95_01630"
     CDS             30326..31366
                     /locus_tag="PLW95_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOV21370.1"
                     /translation="MKKLLLNGCEAAGEGAISAGCKFYAGYPITPQNRFPEYMSYRLP
                     EEGGIFIQSESEISAINMVFGASAAGVRAMTSSSSPGISLKQEGISYLAGCELPAVII
                     NMNRGGPGLGDVTPSQSDYFQATRGGGHGDYRTIVLAPYSVQEIYDFTYLAFDLADKY
                     RNPVLILADGIIAQVMESVNLKPKKKKLPEKKWALTGCKGRKPNSIKSLFLTPGVLET
                     HNWKLARKYERIEKKEIRYEQYFSDDAELGIVSYGTCARIVLESLKKARKNGIKASLF
                     RPITLWPFPYRQLSDFIKSVKKILVIEMNLGQMLDDVKISNTSNKRIFFYGRPGGGVP
                     TPEEIFKEIKKI"
     gene            31363..>32087
                     /locus_tag="PLW95_01635"
     CDS             31363..>32087
                     /locus_tag="PLW95_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOV21371.1"
                     /translation="MKVIYKKPESITDNTTIYCPGCGHGIVHKIIAEVIDQLDIREKT
                     ISIAPVGCAVLSYNYFNFDVTEAAHGRCPAVATAIKRVYPDRIVFTYQGDGDLAAIGT
                     AEIIHCAARGENITVIFINNAVYGMTKGQMAPTTLPGQKTTTTPYGRDTSYHGYPIRM
                     CELLSTLAGPSYIERTTIENVPEIIKTKRAIQKAFTYQVENKGFSFVEVLSTCPTNWK
                     MTPSEAMKWVGDTMKNYFIPGVY"
     assembly_gap    32088..32187
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <32188..32851
                     /locus_tag="PLW95_01640"
     CDS             <32188..32851
                     /locus_tag="PLW95_01640"
                     /inference="COORDINATES: protein motif:HMM:NF019378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1614 domain-containing protein"
                     /protein_id="HOV21372.1"
                     /translation="NIMFFLPFSILLLLIFILLLPFFFLLVQVEIIKIALSKLGLSPY
                     MALLIFLLSLIGSTINIPLFSREVTGVVPNREMFFIFNPFGMPEVQTKQIIAINIGGA
                     IIPLFLCIYLLPKAPPLPTLFATLISIIVCFKISKIVPGMGIVIPSLIPPIIAVLCAY
                     IFSPGNKIPVAYISGVLGVLIGADLLNLTNLGNYPGIMSIGGAGVYDGIFLVGIISAL
                     LA"
     gene            complement(33133..33459)
                     /locus_tag="PLW95_01645"
     CDS             complement(33133..33459)
                     /locus_tag="PLW95_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017380646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HOV21373.1"
                     /translation="MKTVKLSDLAKTIRSKNAGTDKITFDIIFNSADNYEMVRKSGVL
                     SRENISKLYNIDIGRITDFVEFDPAYAIKFTFLRNRPSGSPGEKDIFGCQQYPPLLAL
                     EIPISK"
     gene            complement(33475..34845)
                     /locus_tag="PLW95_01650"
     CDS             complement(33475..34845)
                     /locus_tag="PLW95_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HOV21374.1"
                     /translation="MDRKKMLKFLCSTGHLGTAPIKEASFYAGIEENPDYIIADSGSD
                     DVGPGALGANKSFSSERNQKHDLELMLVESRKKNIPMIIGSAGDCGSKVMVDRYIKYI
                     KEIALKHKLPKFKIGYFYSDLTKNYLKNLLKKGKEIKGLDGRKNLTMGDIDKTNNIVA
                     VAGIHPYHKALDMGVDVIIGGRSGDVAIFAAPAIREGFPEGLAYCLGKIMECASFCAE
                     PYGAKETIIGIITEQDIKVKAMHPEQYCTVASVAGHAMYERSNPYFEYFVGGMLDMSS
                     CQYVQYDKKTCCITGPKFIPAEEIRVKIEGAGKVGERYIGIAGIRDPYSIKNIDRVIA
                     WCRKQVQEQFPGEKYELYFHIYGKNGVMEEWEPVREVRSHELCIVVEGVAATKELAKE
                     ITFCGWMQLFYARLPDVKGTAGSAAFITHETIYDGVAYEWTMNHTIPVNNPMDLFKVK
                     FTYSDE"
     gene            complement(34919..35107)
                     /locus_tag="PLW95_01655"
     CDS             complement(34919..35107)
                     /locus_tag="PLW95_01655"
                     /inference="COORDINATES: protein motif:HMM:NF025063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HOV21375.1"
                     /translation="MGNYIDDLSIFNGLLTDWLVEYNFNRPHRSLDMLTPMEFIEMKK
                     QQNLNKKVLPMYPTHTKI"
     gene            complement(35165..35236)
                     /locus_tag="PLW95_01660"
     tRNA            complement(35165..35236)
                     /locus_tag="PLW95_01660"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35202..35204),aa:Met,seq:cat)
     gene            complement(35227..35826)
                     /gene="rdgB"
                     /locus_tag="PLW95_01665"
     CDS             complement(35227..35826)
                     /gene="rdgB"
                     /locus_tag="PLW95_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOV21376.1"
                     /translation="MKLNFYLATKNRDKIKEIQDILKDTGIEVIKCPEDIMFPEETGN
                     TFEENAFIKANYLKNFLKNNIPVVGEDSGLMVEKLGGLPGVKSARFSSTYRDDRKNIE
                     KLLKMMGNIKNKEDRKGKFITVICLIEDHKRKFFKGEVEGIITFQPRGENGFGYDPVF
                     EIPEIGKTFAQLSVEEKNKISHRSIAFRKLAEYLLKKAG"
     gene            complement(35798..36538)
                     /gene="rph"
                     /locus_tag="PLW95_01670"
     CDS             complement(35798..36538)
                     /gene="rph"
                     /locus_tag="PLW95_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOV21377.1"
                     /translation="MEIKRKGNRKNDEIREIKITKNFIKYAGGSCLIEIGDTRVLCTA
                     NIEEKVPAWLKDSGNGWVTAEYSLLPSSTSERVPRSSNFSGRSQEIQRMIGRSLRGII
                     NLKNLDERTIWIDCDVLQADGGTRVASVTGGFISLVLALNNLKKTNHLRLPIIKDYLA
                     GISAGIVYGNYLLDLDYSEDSVASVDMNLIMTGSNEFVEIQGTGEGYSFSKEQLDNLI
                     TLGEKGIKEIIEIEKKILKDEIEFLLGN"
     gene            complement(36529..38466)
                     /locus_tag="PLW95_01675"
     CDS             complement(36529..38466)
                     /locus_tag="PLW95_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21378.1"
                     /translation="MVSKFKFIILLCFFSSFLYSSEIETIYYTADEMNVNFTENGELS
                     GISLIGNVEVIYKDVHLFCQQAEFNKITGEISCNGNVKVKAEMGEFDVENAKYYLDKD
                     YWILTGASFSSPPFYGKGKNLEKKGNSLIIENGYITTCDLDHPHYRFGADRITYVEND
                     YIKIEKMKVIAGNYNVFYFPKFKYHIKEKQPSTQAKISYKTRIGRNLEVDFFQKNEKN
                     DIDLKEFFHIGSKGIGTGINAKSEENNFNFQALGMKKYDEDKVQPGVIGNFQKNYEKP
                     QGSGDIIFDWRWMYDNDFFNDFFSEKYYSKSKTYNYFSITHNYNNTFFNFNIRENARE
                     EILSVEKLPEIQIFTPYKQISNTPIFFSNNFSLTNFYYNNDNYFRAVEKIMFENKNNI
                     GFFTLSPFISFSGINYNNSSDDKLNYISEVGIKLSTAMIKKQNEKETIFNPSIYFYNR
                     TTKYKPLQLPPLSPYEEMENGKFMEVNFDWNLFKKSEYTGNISISNAYDFNRNEFNNT
                     YIDYEIKINNNFSITGENDFDFNKGSYAFGINDLIFEKENFKYSFGTRYEEDNDICGI
                     ENWVEKKINENWKYRVGIYYDFETENIPVQSYEIWRKLHCLTIDFKMSRDRDNFSFSF
                     LILPTIFFEQNTWKERYSQWR"
     gene            complement(38450..39394)
                     /locus_tag="PLW95_01680"
     CDS             complement(38450..39394)
                     /locus_tag="PLW95_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOV21379.1"
                     /translation="MGKKVLCIGIIVGDFLVKPVRKMPEKGKLNLVDRAELHIGGCAA
                     NTGIDLKKLGCNVAIIGKVGNDNLGKFLINKLKEEGIDTDGIKITDEINTSGTSVLVY
                     PDGERSFLHSIGANSKLRIEDIDFDNMKNFHILHIAGTFVMPEFDGRPTEETLKKAKE
                     MGLITVLDTVWNAEVEHLSLIEGTLKYIDYFLPSYEEAKVIAGKENVEEIGDFLLSKG
                     VRNVGIKMGEKGSYIVNSKERHYFPALNVDVVDTTGAGDGYVAGFITGIVNNYNFEKC
                     GLLANVVGAKITTSVGATSGIISWDDLKNFWTKYGIKI"
     gene            39515..39587
                     /locus_tag="PLW95_01685"
     tRNA            39515..39587
                     /locus_tag="PLW95_01685"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39548..39550,aa:Ala,seq:ggc)
     gene            complement(39766..40500)
                     /locus_tag="PLW95_01690"
     CDS             complement(39766..40500)
                     /locus_tag="PLW95_01690"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOV21380.1"
                     /translation="MSNTKKKVKVIAHRGFSGKFPENTVVAFKEAAKLGVDGIEMDVK
                     ITKDGTLVILHDESVDRTTDGTGKIQELTIEQVKDLDAGSWFSPAFKGTTIPTFEEAL
                     DSIPDNIELNLHLLPNPVITRNVIITLKERKRINNSYLAIHASQIQLAREMCKEIRIC
                     NMRTQTNPVEYIEETHRLKCPILQFFTPSYEVTKELIDKAHSYGIFVNVFFADTENEM
                     KKLIENGADAILTNRPDILIRILKEK"
     gene            complement(40521..43781)
                     /locus_tag="PLW95_01695"
     CDS             complement(40521..43781)
                     /locus_tag="PLW95_01695"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOV21381.1"
                     /translation="MKMLNNLSKLEIIGNMMKKLLYFVFFIDIFFISPIFSQNISKIQ
                     MKLSNNCQNFYLLSARQVSPEVKERIQSMKNTQPWIGIIITDLPDNSQIKGAKVQQVL
                     KNSPAEIAGINNGDIIIELADRQISSVKEVFEIMHSLNVDTEYPVVVLRNNIKLVLKI
                     KPVVVPSQTLAENSPKNNLFFPMKNELTAINTLKYALIDQKTHIITFIGSYDPTYATG
                     DIPYEDILKDLLENPYPSVSIEPTPQEDEILKKVDQLISQDLKKMEKDPAYTDQEMQK
                     LVNLLLKDPTLETDNRRFFNNIASAVGMTGDEFKRLYRASIGEIDLSESELFQLGAKL
                     LKGAGAPEAANGLIAITSGNTPEERLSNMAKALNIYDKYHELDKKILFPEEFRKEAII
                     LCISELCRKMDAPENELQKIISSIRSGRQPVDTIIDYMGKRFTFFISEKMGEKMLNGL
                     VLGPELISKLYNLPIPQSELVFKNVKAGSLLGEILFKADYKLKNINNFPDVKEKFPEH
                     LTEMEFIQKEEKEKNYELPFDIGATSGHTLVPGYVDMKISKDGSIIVFEKSEVKIVGF
                     LKRTFKNQSPETTNFLNETAKKYGEFLTKNYDEYAKIFPEFHIMREVAKLVCLARWAK
                     QNNYTINVLNTSSMYFDQPKNVNGFWAITLQITNGKGSMILIQSGGASFGQEEGEEWM
                     KIQTDVNRTSDVLKQLAGSVVLAEQAVNSAINGNLEDARNLAERSARAMTGEIDLSNL
                     PPIPNIPMPNEAAPYAITTKEAISQINECFETISSVNKDLQYAETIITTSPDKAEEIK
                     EKAIKSQNEAIKKLQQIRDNIIQYKNNPYKAPDLLITLKNNAIVNPIKNISISGQQDT
                     KTQNSSSSISEEEKWEKNCVKWAEELDKVNKEISSTREVLLKLIQSSQLLTEQFKEIE
                     KEATEKYERSINRVGDLLIDIGIEGLQNRYETIYKLAKKLPDKPDELIEKYKYTCSLI
                     QSLKETKETKDFVDLSEIDPRSFIETLEIIKDGISQINGLLELDKTVPGAVWKYLSIT
                     ADTTYDLTEQYYMWKNIILLEKGTDIYSESIKKLTNRLQKLQTSAAILKQKINSEN"
     gene            complement(43832..44770)
                     /locus_tag="PLW95_01700"
     CDS             complement(43832..44770)
                     /locus_tag="PLW95_01700"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOV21382.1"
                     /translation="MIFLKILPVFLIILFGFFARKRKYISLSTIKEMSISITTFFYPS
                     LIFSSFLNNFTTNDIISGWQLPLGTFILMFIGYLTGIFVSKFLEFGTEKEKNTFRFQC
                     AINNYSFLPITIIIVLLGEQKIPKLILSTFGSEISVWTFGIIALTGNKIRKGSFKNLL
                     SVPMIAILISVVLIFIRDRSIITISNQYLKTFSSSILIATDLLGKITSPLALFIAGCR
                     MGDIKKEELFNLKNTILIFLRLFFIPSISIFLFNILGFPYETKLILSIVAIMPTAIAS
                     IVLSEAYNADSEYAAVTTFLTHIFSLFTIPLYLTIL"
     gene            44942..46021
                     /locus_tag="PLW95_01705"
     CDS             44942..46021
                     /locus_tag="PLW95_01705"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOV21383.1"
                     /translation="MKTYKVGIIGFGFIGKVHAYGYENLKYYYPNSPFRVEIEGISTS
                     REETAKNVKETYGFETTSDYRKLIENKEIEIIDISSPNIYHFEQLMYGIKNKKHIYCE
                     KPLVCTIEETLELEKKLKNFDKNHQVVFHNRFFPSTIKTKQFIENGSIGNPTIFRIAY
                     YHSGSVDKNKPIGWKQEKGAGVLLDIGSHVIDLIYYFLGNFKNVSGKSKILYPERKTK
                     NGKIVKVEVEDHIVINAEMENGAIGIIEASKIATGTEDELKYEIYGTEGAIRYNSTQP
                     NYLNFYNRKETESGFKKIPTLGNYPESNFPGPKFTTGWIRGHIHSIYCFIKSIYENSP
                     AIPSFFDGIYNMKIIEDIKSGDENA"
     gene            46014..48371
                     /locus_tag="PLW95_01710"
     CDS             46014..48371
                     /locus_tag="PLW95_01710"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HOV21384.1"
                     /translation="MPDEIAKKKKRGKILQSKSHDVFLNYPARDWGEGIPIGNGFLGS
                     IIYGDPSMLSFTLNRSDVWDYRYKEFDPEGKLKFNQLKNLLKKRNEKAYKDFVKETED
                     HSSKYPYPAPQTCGRMQISFKSDAGRYCQRLSPEIGKTFIEFSYNKKKRVIEVFIPPE
                     LPIMFVKIKGKNLEPFSIFLNRDGLSIIPELIKSEIPSEKIKIEYAGNKELFKMIGKF
                     PDGLYFEVGIKEKEGIFSSIDTGKGKVDIHPDGKNEITIVLGVVSGYKREKLDEEIAK
                     VINNSSKNYTLLNTRHLQEWGKFWSQSSITIPDKLLEHIWYISQYFLNSTLKYANQAP
                     GLYGLWTYHDITPWRGDYHLDRNLQAVLEPLFSSNHIELADIYYDFFISVLPEIRKQT
                     KKFYRWDGISFPGSIGPKGENMGGYFPVSHWEGTSAWISLLFWWKYRFTMDNDFLKEK
                     AYPFMKECGIFYEKYLGEKREDGKYHLWPSYLPETGENSMKAWGTDSAIDIGLLRFLF
                     GALIKAAKILGKDKEDIKKWEEILSGLPDYPTWDGAIVDYQEWPELYEKESIFTDDLR
                     IAPIYPIGDITQDSPDELLELGKRTFYSLGKHHARIWLLTESVVASRLGISELSYGYI
                     KRWAQHITPQGIGTYSTGISIGWRDTSLNDGIRKLYKLVPWNYSVFGIEFPSNFISAL
                     NEMLLQSCGEKIRVFPAIPKEWKEVKFENFRAERAFLISAYMKDGVVNKIVVKSIKGE
                     ELLLVNPEPARKFCIKKNGKKLGIGEGKIFRLKTTPSSCYEFTIK"
     gene            complement(48466..50088)
                     /locus_tag="PLW95_01715"
     CDS             complement(48466..50088)
                     /locus_tag="PLW95_01715"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HOV21385.1"
                     /translation="MNSLLVAGIGIFLFLIAYVFYGRYLEKLWEVNPDEKTPAYKKED
                     GVDYVPVKHWSILFGHHFGSIAGAGPILGPVIAAVCWGWCPTFLWIVFGSILLGGVHD
                     FSSLMVSIRNEGKSIGDISSSVISKRGKIFFSIFLWFTLILVVAVFAAVTATTFIEEP
                     KIVIPTFSLLIIAIFFGVAVYKFNLPQIHATLISLILYVLLFFLGSRYPVVISPAYAT
                     KAWIVILLLYSLIASVLPVNLLLQPRDYLSSFLLFFGLLFGYLGLVITHPQIHTPNFV
                     SYSSSNGPLWPMMFVIIACGAISGFHSLVSSGTTSKQINNEKYAKNIGYGAMLVEGVL
                     SILALICVTAGLYWNEPNSLLNYPSLMKGGNWIGTFALGYGQVTSPIFGSKFGTMLAI
                     VIINSFVLTTLDTATRITRYISEELFGDTFKINIFKNRYFATIFIIIIAGYLAFGSWQ
                     KIWPVFGASNQLVAAVVLLICALYLSERGKNSKVLFYPSIFMFLTTIVALIIQAKDFY
                     LNKKLLLGNIASIIVLLALFFIVESIREKRNH"
     gene            complement(50358..>50852)
                     /locus_tag="PLW95_01720"
     CDS             complement(50358..>50852)
                     /locus_tag="PLW95_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the formation of acetyl-CoA from pyruvate
                     and coenzyme A; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HOV21386.1"
                     /translation="IQRSSATPFGAHTSTSPSGKVIPGKLQWQKNLPEIMVAHNIPYV
                     ATSIPGKWNDLVNKVEKGTTFDGFAFIDILSPCRLGWPHDPKLTLEISRLAADTCIWP
                     LYEVVEGKYKLTYKPKEKKPVIEWFKLQRRFSHLLKPENKELLDKIQQEIDKRWEALL
                     KRSE"
BASE COUNT        17372 a         7720 c         7346 g        18314 t
ORIGIN      
        1 ttttgtcttg tctaacatac cttttggagt agatttatta tgtctcaatt cgaaacattg
       61 acagtgtaat ttttttagat aaaatattag taatatgatg ggaaaaataa tatttgtaaa
      121 aggaagaaaa aaatagatat agaattaata aaaatatcag aagacaattt aaaagataac
      181 aaaggtaaaa taaaacttat tctgtaagga gtaaaacaaa tgaagttttc aacaaaaaca
      241 agatatggag taagagcaat gatagaactt gcacttaact ttaatggtac acctatttct
      301 ttaaaagata ttgctgagaa acaggatatt tctgaaaaat atcttgaaca aataatgcta
      361 atattgaaaa aagcagggtt agttgaaagt attcaaggga caaacggagg ttttatactt
      421 gttaataaac catctgaaat taaactatct gaaattgttg aagttcttga agggggtttt
      481 tccccagttg cctgtgttga taaagaaatc ttatgtaaaa aaagtaaagt ttgttctgca
      541 agaaatgtct ggataaaggt aaaaaatgca ataaaagata ttcttgattc aattacatta
      601 gaacaattag ttgaagagga aaagaaaaaa cagagtattt attatattta gtggtaaaaa
      661 ttggagagaa aatgaaggta agtaaaaata taacgaattt gataggcaat acacctcttg
      721 taaaattaaa taaattaaca gagaaatgtt atgctgaaat ttatgcaaaa cttgaatttt
      781 ttaatccctg tggttccata aaagatagag ttgcagtaaa tatgattgaa gaggcagaaa
      841 gaaaagggat aataaataac gatactgtaa taattgaacc aacaagtgga aatacaggta
      901 ttggacttgc ttttgtatgt gctcaaaagg gatataaact tattttaacg atgccagaaa
      961 atatgtcaac tgaacgaaga aaacttcttt ctttatttgg tgcagaggtt gttttaactc
     1021 ctattcgtga agggatgtct ggagcaataa aaaaggcaga aaaaatttct aaaaaatata
     1081 aaaaatcatt tatgcctcaa cagtttaaaa atcctgcaaa tcctgatatt catagaaaaa
     1141 caacagcaga agaaatatgg aaagatacag atggaaatat agatattttt gttgcaggaa
     1201 ttggaacagg agggacaatc acaggagttg gagagtcatt aaagaaaagg aaaaaaagtg
     1261 ttaaaattat tggtattgaa ccagaaaatt cgtctgttct ttcaggagga aaaccgggag
     1321 agcataaaat tgagggcata ggagcagggt ttatccctga tattttaaat ataggtataa
     1381 ttgatgaaat aataaaggtc tctgataaag atgcaattga aacgacagaa aaattagcaa
     1441 aacaagaagg aattttagca ggtatttcaa gtggagcagg aacatttgct tctattaaaa
     1501 ttggaatgag aaaagaaaat aaagggaaat tgatagttgt tattttccct gatacaggtg
     1561 aaagatattt atcggtttta aatgaaaagc gggcagaaaa ataaggagga aaaaagtgga
     1621 aaaggaaata tcaaaaggta ttattgaaag ttatatgaag gaatttattg ataatttaga
     1681 ggttgatgtt gcaattgtgg gtgcaggacc ttcaggactt atttgtggtt attatcttgc
     1741 aaaaaaaaga ataaaagtag caatttttga gaggtcatta agagttggag ggggaatgcc
     1801 aggaggggga atgatgttta ataaaattgt tattcaaaaa gaggcaaaag aaattttaga
     1861 tgaatttgaa ataactctta aagaatatga aaatggactt tatattgcaa attcagttga
     1921 aacaatatcc tcttttgctt atagaacatt gaaagcagga gcaaaaattt ttaatcttat
     1981 aagtgttgaa gatgttgtta taagagagaa gagaataaat ggagttgttt taaattggag
     2041 tgcagttgga atagccaaat tacatgttga tccactggtt gtcttatcaa attatgttgt
     2101 tgatgcaacc ggtcatgatt gtgaggtctg caggattgtt gaaaaaaaag taggtggaat
     2161 tgaagtaaaa ggcgaaaaat caatgtgggc agaaaaaggg gaaaaagaaa tcttaggtaa
     2221 tacaggacag gtttatcccg gtcttatggt ttgtggtatg gcagcaaatg ctttttatgg
     2281 ttctccaaga atgggagcaa tttttggtgg tatgcttcta tcaggaaaaa aggcagcgga
     2341 gttggtagaa agggaggttc ttaatggcaa aaacaataga agagataaat gaaaaaataa
     2401 aaaaaggaaa agttgttgtt gtaacagcag aagagctaat tgatatgaag aaaagtgcaa
     2461 atagtaaaaa attatcagaa gaaatagatg ttgtaacaac aggaactttt ggaccaatgt
     2521 gttcttctgg tcttcttata aattttggac attccaaacc aaaaataaaa atgggtggag
     2581 gaagtgtcta tttaaacaag gtacctgctt atgcaggact ggctgctgtt gatgtttata
     2641 ttggagcagc agcacttcct gataatgacc caagaaatag taattatcca ggtgaattta
     2701 aatatggagg aggacatgtt attgaggacc tagtaagtaa taaagaaatt gaacttaaag
     2761 tagaaacata cggaactgat tgttatccaa gaaaacatct taaaacaaaa ttgaaactaa
     2821 atgacttaaa tgaaacaaca ctttttaatc caagaaattg ttatcaaaac tataatgtgg
     2881 cagttaatct ttctgatagg acaatttata cttatatggg aattcttaaa ccaaatttag
     2941 gaaatgcaaa ttattcttct gctggacaat tatcacctct tttgaaagac ccatatttaa
     3001 aaacaattgg gtctggcaca agaatttttt taggtggtgg aattggttat gttgcctggt
     3061 ggggaactca atttaaccct tctgttaaaa gaaaagaaaa tgggatacct attgaaggtg
     3121 gttgtacact aactttaata ggagacctta aacagatgag ccctgatttt ttaaaaggag
     3181 catcattaac agggtatgga gttagtttaa tagttggagt tggaattcct atacctattt
     3241 taaatgaaga aattttaaat tttgcttcaa tacctgatga ggaaatttat gcaccagtag
     3301 ttgattatag taatgattac ccaaatgcaa tagaaaaagt tatttgttgg gtaaattatg
     3361 cccaacttaa aagcggcaaa gtagaaataa tgggtaagaa agttccaacc ggttctttat
     3421 caagttataa aaaaagcagg gagattgcaa atattcttaa aaattggatt ggaagtggta
     3481 aatttttatt aacagaacca atctttcatt ttcctggtcc tgatagtgga tatatgccaa
     3541 aaccaatgaa aatttaatag gagaaaaaat ggaaataaaa aaaagatatg ttatgcactt
     3601 tccaccaaat ttaattgata aaccaataat ttcagatgca gtaagaaaat ataattttca
     3661 atttaatatt ttaaaagcat atataactcc tgaagaagaa ggtactcttg ttattgaatt
     3721 gataggcgaa gagaaaaatt tagaaaatac agaaaatgtt ctaaaagaaa tgggagtaaa
     3781 agtccagaca atagaaagtg atattaaaat gctcaaagaa aaatgtactg attgtggtgt
     3841 ttgtgttcct ttatgtccaa caggtgcttt aaaaaaagaa gatgacttta ctattagttt
     3901 tttgccttca aaatgtgttg catgtgaaat ttgtataaaa gcatgtccaa caaaagcaat
     3961 gatatcaaca atttaaaatg gataaagaaa taagaaaact aaaagaggag aaaaatgcag
     4021 taattcttgc acataattat caaataccag aagttcagga aattgccgat tttgttggtg
     4081 attcttttga attgtcaaaa aaggcatctg agataaaaga cgcagagata attgtttttt
     4141 gtggagttaa atttatggca gagacagcaa aaatactttc tccttcaaaa aaggtattac
     4201 ttcctgaaat ggatgcaacc tgtcctatgg ctgatatggt aaaagttcag gatgtaatta
     4261 aaatgaaaga aaaagagcca gatgcatggg ttgtatctta tgtaaataca aatgcagaag
     4321 ttaaatgtct ttctgatgtt tgttgtacaa gtggaaatgc agaaaaaatt gttagaaata
     4381 tccctgcaaa aaaaataata tttttgcctg ataaaaattt atgctggtat gtccagcaaa
     4441 gagtaaaaga aaaagagata atatgctggg atggattttg ctatgttcac cagcaattta
     4501 caacagatga tataaaaaaa gcaagaaaaa agtatcctga ggcagaaatt gttgttcatc
     4561 ctgaatgcga cccagaagtt caaaaattag cagatggtat ttattcaact tctggaatgt
     4621 taaaaagagt taaagaatca aagacaaaaa aatttataat aggaacagaa gaagggatta
     4681 tttatcggat gaaaaaagaa aacccagaaa aggaatttta tcctttagga gataaaaaag
     4741 tttgttctga tatgaaaaaa ataacttttg aaagtgtaaa aaggtcatta gaagaagaga
     4801 tatatcaaat aaatttggga aaagaaataa ttgaaaaagg taaaatatct cttgaaaaaa
     4861 tgtttgaata ttcttaattt actgaatata agttattgat tttcaagcac ttgttaactt
     4921 acgaagttaa caaaaaataa aatgaaggaa aatgggaaag ttatagaatt aaaaggagat
     4981 atagcagttg ttaagatgga aagaaagaat aaatgtgaga aatgtggatt atgtgaaaaa
     5041 attgctggaa gagacccttt tatagaagta aagaataaaa ttgatgcaaa aattggtgat
     5101 gaagtagaag tagaaataaa agaagatgac cttctaaaaa tttcaatttt tatttttggt
     5161 tttcctcttt tggggtttat tttagggata attagttctt atttttgtaa aaatattaca
     5221 ttaaaaattg ttgtttttct tgttttttta ctatctttct ggattgctgg ttttaaaaaa
     5281 ggaaaaaatt atgcagaaaa cacaaaacct cacattgtct caaaaattta atttctgaac
     5341 aattaaacat gttttaataa tagagtttta tgggaggaat tatggagtta aaagaaaaag
     5401 ttgagaaagc aatagaagat gtaagagaat ttttacagac agagggtgga gattgtgaga
     5461 tagttgatgt tgaaaatgag aaagtaaaag ttcgtctaaa aggtacttgt aggggctgtc
     5521 catttgctgt tattacttta aagatgagaa tagaaagtat tataaaagaa agagtccctg
     5581 aaataaaaga agttgttgaa gttgactaat gatataaaat taaaaggaat ttcaatgcat
     5641 gaattttcag tcgtagagaa tctaataaaa attttaaaaa aagtgataaa agagaataac
     5701 gcaaaaaaag tattaaaagt aaatttaaaa attaacccat ttagttgttt agaccaggag
     5761 aatttaaatt ttattttttc ttcaattgta aaaaataatg attttttaga aaatacaaaa
     5821 ataattataa aaagaggaaa agacccatta tcaagagaat atatagttga aaatgtagaa
     5881 attgaaattt aaaaatgaga gaaaaaggag gaatttatga tggatatttt taaaataaaa
     5941 aatgcaaaaa caaaaagaat ttcaagttat gatgttgaag gaagaaatgc tgatagatgg
     6001 attataaacc caggagagaa aaaagtactt gcagaaattg aagggaccgg ttgtattact
     6061 catatatgga tgacacaaag cggtaattat tctttatata gagatgttct tttaaaattt
     6121 tactgggatg aagaagaaaa tccaagtata ttagttccac ttggagattt tttctgttta
     6181 ggacattctc ttgttaattc tttttgctgt ctaccattta gtgcatcaac aaatacacca
     6241 tatatttttg ggggtggctg tgctttgaat tgctatttgc ctatgccatt taacaagggg
     6301 gcaaaaattg aaattttaaa tgagtcagac caagcatatg gtcagtattt ttatattgat
     6361 tatgaaattt atgaaaataa ttttgaagga gaaataggat atttacactc acaatggaaa
     6421 agagaaaatc ctacacctgg atggggaaat gagtttaaag taaatatacc agaggttgat
     6481 atagtaaata aggaaaaaat tgcttataat aataactatg taattatgga aggtgaaggg
     6541 agagggcatt ttattgggtt taacctttct gtaacaaatt ttcattctga ttggtggggt
     6601 gaaggagatg aaatggtttg ggttgatgga tataaatggc cacctgattt acatggaaca
     6661 ggaagtgaag attgttttaa tcaagcatgg ggcatgcaag aaaatgcttt cttatttaat
     6721 ggttcgtcaa tttatgaaaa aaattctatt tttgggcatt ctctttatga tgggaaactt
     6781 caagggggtt atcagacatc atatattttc tatattacaa atccaatcca ttttaaaaaa
     6841 tcaataaaat tcacaattga acatgggcat ggaaatcatc tttcaaatga ctattcatca
     6901 actgcttatt ggtatcaaat tgaaccacat aagtcatttg gaattttacc ggtggaaaag
     6961 agaaaaccaa ttttattaaa atttgaaaat tctcctcaac tacaaacaga aaaaaggaaa
     7021 attatattaa cagaggaaat gaaaaaaaga aaaaaataaa aatgttattt atcataaaat
     7081 gtcttttagc aggtggaatt gctggttttt tatgtgggtt attaggaatt ggagggggtt
     7141 ctataattgt tcctgttctg tttttcttct tctctgtccc aataaaaaat gcaattgcaa
     7201 caagtttaat ggttgtcctt atttcagcaa tttctggttt tttagtacat cttaaaggga
     7261 agaataccga ctttaaactt gcattttacc ttataatttc aggggtaatt ggtgcttata
     7321 ttggtgctta tttaacaggt attttaccag aaatgattgt gaaaggtttt ttcatggttt
     7381 tgctaattgg gttagggtta aaacttcttt ttcaaaaaga aaatgagaag gaggataata
     7441 aagaatattc cctggatatt ttaaaaacac tttcaattgg ttttatatca ggcattgttt
     7501 cagggctttg cggagttggc ggtgcagttc ttttagttcc actctcatat attatcttaa
     7561 aagtcccaat aaaaatttgt gttgggacaa gtttaatagt tgtttttttc aatgctcttt
     7621 caggtgtttt tgcttatgcc aaaatgggat ttattgatta tagaattggg ttgacttttg
     7681 gaatagtagg aataataact tctcctattg gagcaaaagt cagtatttta actccaagag
     7741 agaaattaag aaagatattt gcaatttttc ttattttaat gccaatttta cttttattga
     7801 gaaaatgaaa ataataggaa ttgatgaaaa tggttttggt tctcttactg gaccgcttat
     7861 tattacagga acttcaatta agtcagaaga aggaaaatgg tttgccagtg tttgtgatag
     7921 taaaaaattt ttctcatcaa gaaagacaaa taactttaaa aaacttgaag agtttgttat
     7981 ttctctttac tattgtataa aaaggaaatt accagaatcc agcaaagaag taattaattt
     8041 tttctctcct gattttaaat gttctggaat gtcggatatt tgttcggtga atattcctga
     8101 taattttatc tgggctgata tagaaaaagc aaaaaaacag agtgaaaaat ttattgaatg
     8161 gatggaaaaa gaagatgttt ctataaaaga tgtaaaatca aaaattatat gtgcaaaaaa
     8221 attcaatgaa ttgataaaaa atgggaattc aaaatttata atagaccttt tttatttctg
     8281 tgaaatttta aaagaaatgg gaagggaaga ttattttgtt tatgcaggga aaattgggtc
     8341 aatgaggtat tattcaaagt atttaagata ttttttttct aactatgaaa tttatccaga
     8401 aattgaagaa gaaaaaaagt caatttatgt attaaatgga aggagaaatt ttaagttggg
     8461 tttctttttt aatgttgaag aagtatcttt tgcttcaagt atatcttcaa ttgttggaaa
     8521 atacataaga gaaattttta tggaaagttt caggaaatca acaggaattg aagaaaaaat
     8581 ttctggatat cgggataaaa aaacaaaaag agttatttca aatttatttt cttttcctca
     8641 ggagtgtgtt ttaaggatta aatgaattgt cttgatattt caattgattt ttcaactctt
     8701 ttaatatttt caaattcaac aataatttta ttactttctt ctgaaaattt attgttagtt
     8761 tctattccat ctatgattgt ttttacaaaa tttgtttttt tcttcttcca ataggaaaga
     8821 taaattttcc taacttcaat aaacccttca tctatttcta ttttgaaatt ttctcttcct
     8881 ggtaaaattt tataattata aacaccccat ccagttccac aagagaaaaa aatagaaaaa
     8941 ttttttctga aaagcaaggg attgaaaaaa atttcctttt taacattatc aatccaaaag
     9001 cccaaatatg agagaataag tccccatcca gaaagccctc ttgcgtaatg atgtccacat
     9061 tctaaatgat taaaaggatt cctttttatt ccatcatatc tgttatccat cccattaatt
     9121 attttaagac attctttata atatcctctt tttagaagaa gacatgcaat aacaaattct
     9181 tcccctgtga aaacttcatc gcaataaggc atgggtattt ctggcttttc gccttctttc
     9241 catgtgcata caagaagtcc tttttcttca ccaatagcaa atattctcat ataattctga
     9301 tgttgtgaaa agttctctaa aaagtttaat tttaaaattg aaaataaagc cttatttata
     9361 tatttttctt caacaagaga ccctaaattt aaagtatcag caaaaaactg cccaagaagt
     9421 tgcatcgaaa gacatccatt taaaaattga taaggtggga caggatttat actacatttt
     9481 tgaataaaat atctcccatt ccatagtttt tcaattatat ttttttgtcc tttttgataa
     9541 atttcattgc aaaaagaagc aaaattgttg tcactgttat attctgccat ttctatcatt
     9601 actttaagcg ctgcaagata aataaaagaa gtaaaaggat tatctccata aaactcaata
     9661 tcataagtat tgtgttgttg cccttccata agtccatctc tatttttatc ccagttttca
     9721 aaagcatatt caatagtttt ttttgcagat ggatataatt tttttaaaaa ttcatcatca
     9781 ccagaaattt tccattctct gtaaagtttc aatattgttc ccatttgtcc atctgctgct
     9841 ggtagagata accatctttt tatacccagt gggacatttg ttctaaatgc catgtatcca
     9901 ttttcatctg tattatataa gtaatcaact tccctttgac ttcttgaaag ggaagggaaa
     9961 aggaaaggaa ctgtaaaatc ataattatga acatgggtgc agtttaaagg acaacaacca
    10021 gaattatcag aacaaccctc aaatccataa aatttcccat cagaagttat aaaaaatgta
    10081 ttacttctta atgtatttaa ttgggctgaa gttgtattta aaaaactttc aggtaaagat
    10141 tgagaaaaaa atctttcagc aaattttttg cttttttctt caagatatct tttatttttt
    10201 ataaagtatt taattacatc cattgaattt gaaaatttta tagaataata attttttaaa
    10261 attttacctt ttacttcttc atcaagcccc cagtaattta tacgatttgg aaaataccat
    10321 gaaagataaa aattaatatc tgtttcttct tttggtgcaa gatttttatt aactccaaga
    10381 gaagcccaat atgtttggct atcaggactt attttgggag aaacaggttc aaaatttcct
    10441 attttgaatt ctttccagaa gttttttaag tcatcccacc atccttcatt tttccattga
    10501 tttttgtaag aaaccgaaat agcatctgta aaaagagaaa gattaccata tctctcactt
    10561 tcctcattat atttttttga tgtgaaagaa atacctttta aattctctgt ctcaacaaaa
    10621 ttatttatgt tctgcccaaa gcatttattt atataactat ttaatttttc agttccatca
    10681 gttccaacag ggttcatcat tgaaaaagaa aggaagatat ttattttatc tcttgtaaga
    10741 ttttttattt tataagagaa aattacaatt ggtaaggaag aattttcaac atctcctggt
    10801 ataaatgggc tccatgcatt taaggataca gaaacaccta tacttctatc aaaaaaatca
    10861 ataatagcaa aagggaaaaa atttgtaaat atacattcct caaaatgtgg gtatccttcc
    10921 attctaatat ttttaaaacc atgacttcct tcaaaaggag gaaatggctt tcttgcaact
    10981 attttttgaa aaactttcct cttttgttca acccttaaag caacaaagtt cccatacatt
    11041 tcattacttt ttgaaggtct attgaaaatt tcccagtcag ttattgagcc agttccttca
    11101 acagaaacat tacctgttcc aataccacca agtaaaaatt ttatttgttt taaattttct
    11161 ccataatatt ttatattcat ttttttcctt tgttcacatc tcttcaagta gagaaatatt
    11221 caataaatcc agtgataaag aaaaaattgt atataaccct tttattaaga acaatctgct
    11281 ttccgttgtt tttttatctt ctccaacaac tctatatttg tgataataag aatgaaagat
    11341 ttttgctaaa tttaaaagat attcacacaa aaggtgaact ccgtatgttt tcaccacact
    11401 ttcaattata tcaggaaatt tccacatttt tttaagcaaa atgagctctt cttcctcttt
    11461 taaataatct gggtcaaatt cggtttttcc tattgtaata tttttacttt ctgcaaaatt
    11521 caaaatatgt tttattctca cataagcata ttgaagataa aagacaggat tctcagttga
    11581 ttgttttttt gcaagttcta tatcaaagtc aagatgacta tcaacttttc taaaaagaaa
    11641 gaaaaatttt gatgcatcag gtcctacttc atctataagt tgtttcaatg aaataaattc
    11701 tccttttctt gttgacattg atagtttctc ttttcctttg tataaagttg taagttgaac
    11761 tatcagaaat ttgagttttt ctggctcaaa acccattgcc tgaactgctg ctttcattct
    11821 tgggatatat ccatgatggt ctggaccaac tatatttata agtattgaaa aatttctttc
    11881 aatcttatat ttgtgatatg caatatctgt tgcaagatat gtataggttc catcactttt
    11941 tataattact ctatctttat catcgccaaa gattgaactt ttaaaccacc aacttccatc
    12001 tttaaaataa atatagcctt tttcttttaa ttcatttatt attttgtcta ctttcctttc
    12061 ctttataaat atactttcat ttacccaact atcaaactca actccaaaat tcctcaggtc
    12121 tctttttatt tcttccagaa ttctctcata agcaaatttc ttaaaaaatt catcatctac
    12181 cttttcttct tttatttctt ttgctaactg aataagatat tctccatgat atccctcttc
    12241 aggtattgtg tatttttctt ctccttttaa ttgataatat cttgccttca atgactctcc
    12301 taaaaggtca atttgttttc cactatcatt tatataaaac tcttttacaa cagaaaaatt
    12361 agcaaatttc ataatccttg aaagtgcctc tccaaaagat gcctgtcttc catgagcaat
    12421 tgttaaaggg cctgttgggt ttgcgctcac aaattcaatt aaaacatttt ctccccttcc
    12481 gatttcagat tttaaaacat ctttccctgc tttaacgagt tcttttaaaa agttaaaata
    12541 aacatttttt gagacaaaga agtttatata gttattttta atttcaattt tctcaactat
    12601 atctgatact attggtaata ttttttctct tattttttct gcatagtcat tgccaagttt
    12661 aaaaacaata ttagttgtaa aatctccgaa gttttctttt tctgacggga atacatcaac
    12721 atcaattact ttatttgaaa tttcttctat aacttttttt atgccttctt caattctttt
    12781 ctttattttc ttcattttct tcttttttta tttccgaaag taacttttca attctaaatt
    12841 cttttctttc atcaagttca aattgaattt ttggagtatc tcttagagat attcttttcc
    12901 ctaatttaaa ttgaatatac cctgttgcat tttctattgc tttaaatgac ttttgttttt
    12961 cttcatctgt tccaagaaaa cttataccaa tttttacaaa tttaaagtca ggagtaatat
    13021 ttactgatgt aattgtgaaa aaaccaacat tagggttaga tacttccctt cttattatat
    13081 cttctacttc ttttcttaaa agatgttcta cctttttttg tcttcttgta ataatttttc
    13141 cctccttttt tctaattaac ttaatttttg cttattatat tttacctgaa tgagatataa
    13201 tttcaaattc taaaagccaa tttctaagag ccaaacttga ttgtaaaaat gcatagaggt
    13261 aaaataaata tatgaaattt aaagatatag aaagatgggt aaaaggaata attgtttttt
    13321 ttattgcact ttatcttttt ctttctctta tttcttttga cccatttgac ccgaccttca
    13381 aattctgtca aattggttct ccagttaata cagaaatatc acattttggg ggtagagtag
    13441 gttcatatat tgcagggctc ctgattttca tttttggtaa aggggcttat ttacttgttt
    13501 ttctccttct tttttctgcc tataatgtta tttggggaga aaaaataaat ttcgggaaaa
    13561 aaattataag catatttggt attgtttttg ttttttcaat ttttttatct ttacaaacaa
    13621 aaaatttttc aggtggtttt ttaggaggtg ttttcacacc ttatttgaag gaattttttg
    13681 gtgaaaaagg aatttattta atccttattc ttttattcgt tgttttcttt ggtctggtat
    13741 ataaaatgtt tgtttcccct tttcagtttc tttttgcaag agatgttttc tcaacaacag
    13801 cagaaggtaa agaaatgttg agaaaaaaac cagtaatgaa aaaaagagtg ataaagaaaa
    13861 atgaacaggg gccagaaata gaagaaaaag aagttcaaaa agaaaaaagt gaacaaaaag
    13921 ttattattac aactaaacag aaaaattttg ttcttccgcc tgttaaactt ttaaaatcag
    13981 atacttttcc tcagaaagaa acaaaagatg attttgaaag gtatgctcag gtaattgaag
    14041 aaacactttc tgagtttgat attgaaggag aagttgtcca gatcaaccag ggtccaaggg
    14101 taacaatgtt tgaaattcaa ttagcacctg gtattccagt tcaaaaaata tttaaaatac
    14161 aggacaacat agcaatgaat ctaaagacaa cttcaatccg tattgttgct cctataccaa
    14221 ataaatcaac agttggagtt gaagtgccga atagagaaat atcaactgtt tatttaaaag
    14281 aattacttgt cagtaaagag tttcaaaaaa gcccatcaaa actgacaatt gcaattggaa
    14341 aagatattat gggtagaagt gtcgtttctg accttaaaat tatgccacat cttttaattg
    14401 caggagcaac tggttctgga aaaacagttt gtcttaattc tcttataaca tcaattcttt
    14461 ataaagcaac tcctgatgaa gttaagttta tacttattga cccaaaaatg gttgaactta
    14521 ccctatataa tggaattcca catcttttat gtccagttgt tatagatatt aaaaaagcag
    14581 tcaatgtttt aaaatggtta atacaggaaa tggagaggag atacaaactt ttttcagaaa
    14641 aaagagttag aaatatagag acatataatt tacagttcaa ggaagaaaaa ataccttatg
    14701 tagtcattgt aatagatgaa cttgcagacc ttatgattgt tgctaaaaat gaaattgaac
    14761 aaagtattat cagattggct caactttcaa gagcagcagg tattcatctt attcttgcaa
    14821 cacaaaggcc ttctgtaaat gtaattacag gtgtcataaa agcaaattta ccctgtagga
    14881 tttctttcca ggtaacctca aaatttgatt caaggacaat acttgataaa attggtgcag
    14941 aaaaacttct tggcagaggc gaccttttat ttattcctcc tcagagttct attccattaa
    15001 ggattcaggg cagttttatt tcagatgaag aaattgaaag tatatgtaat ttccttaaaa
    15061 gtcaaagtag cccacaatat aatatggaaa ttcttgaaaa aaaattattt gatgaaacag
    15121 aaatagaaca tttctcttca tcctctgcaa aattagatga tgatatcctt tatcaagaag
    15181 caaaaaatat tgtattaaca acaaagattg cttcaatttc tatgttacaa agaagattaa
    15241 atataggatt taatagagca gcaaaatttg ttgagaaaat ggaagaagaa ggaattgttg
    15301 gaccatatat tgaaggtaag ccaagaaaag tactaatttc taatgaaaat agagaaagat
    15361 aaaataaatg ttttattgaa agaaacaaga gaaaaaaagg gaattgggat tgatgaaata
    15421 attgagaaca caaggattcc acaaaaatac attaaaataa tagaagatgg acaatgggaa
    15481 aaattcccaa gtcaaactta tttacttggt tatttaaaaa tttattgtga atatcttaat
    15541 attaaaaaag aaataattga tacaatttta tctgaattta aacaggaaaa taaaaaggaa
    15601 aaagaggtaa taaaagaaga gaaaaaggat aatgtagaac agaaaaggga aaaaatcctt
    15661 ttaataattc tccccttaat tttcttctca atatatttct tcactcttta cattctttcc
    15721 gatataagtt caaatttata aaatgggaaa aaaggtctct ataataagtt taggttgtgc
    15781 aaaaaatctt gttgattctg aaaatatact tgctttactt ggagaggata aatttgtaat
    15841 tactgattca tataaagaaa gtgatattgt tattataaat acttgtgctt ttataaaaga
    15901 tgcagttgat gaatcaataa gagtaataaa aaaaattgct gaaaagaaaa atagaaagaa
    15961 aattattgtt tgtggatgtc ttgtccagag gttcaaagaa aaaatcttca aaattgctga
    16021 tattgatgga gcaattggaa caggggaccc tgggaaggta tttgaattgg tcaaaacatt
    16081 aaaagataat aaaaaaatct tgctggttga tgataaaaca tttgtaacaa gtaaaacata
    16141 tccacgattg ataactacat ttccatatgc ttacttaaaa atttctgagg gatgtaataa
    16201 taaatgtaat tattgtctta taccatattt aagaggaaac cagagaagta gaaaaataaa
    16261 agatatttta aaggaagcag atgacctatc taaatcagaa ataaaagaaa tagtcattat
    16321 tgcacaggat acaacaaatt atggaactga tttaagatat gggacaaatt taaaagagtt
    16381 attagttgaa attacaaaat atgattttaa atgggtcaga ataatgtact gtaatcctgc
    16441 ccgcattact ggggacctca ttgaagaaat atctaaaaat gaaaaaatat gtaaatatct
    16501 tgatatccct ttacaacatt ctcatccaga aattatcaaa aaaatgggaa gacctgtaat
    16561 taactataat aaaattattg aaaacataag aaaaggaata aaagaagtaa gattaagaac
    16621 aacttttatg attggatttc ctggagaaaa agaagttcat ttcaagcatc ttgttaattt
    16681 tataaaagaa aataaatttg atagatgtgg ttttttcaaa tattcaagag agaaaggaac
    16741 atcatctttt gattttgacc aacaaattga tgaagaaata aaagataaac gacttgaaaa
    16801 agtagttaaa atacaggaaa ggatttctaa aaataaactc aaaaatatgg ttgggaaaac
    16861 atatgatgtt ttaattgaaa aaaaagatgg gcaattttat attgggagaa ctgaatatga
    16921 tgctccagaa gttgatgggg ttgtatttgt atcaggcaag aatttaaaat tgggtaattt
    16981 ctataaagta aaaattacct cttcttctgc atatgatatt tctggtgaag ttattattta
    17041 attttttata ggattaccat ttgttcaaga cgaataccaa atttcttatg catataaatt
    17101 cctggctcaa ttgtaaaaac attccctttt tcaattatat cattacttcc aggcgcaaga
    17161 tttggttttt catgaatatc aattccaact ccatgcccca atgaatgaat aaaatatttt
    17221 tctaaattat attttttgaa aatagtgatt gctttctggt gtacatcttt accccttatt
    17281 ttcccctttt gttttttctc ataaatatat tcagtagcaa tttctctaac atcttttaca
    17341 attttatata cattttttat ttcatcagaa acatctccat aaaaaaaggt atttgttaaa
    17401 tcacttttat atccacaaaa atctgctcct atatccacta ctattacttc ccctttttct
    17461 attgttttgt tttttgcttt atggtgtggg taagaagaat gaataccaga agcaacaatt
    17521 ggctcaaaag attcactttc tgccccattt ttatttaata ggtattttat ttcaccaaga
    17581 aggtcaagtt cagtaaattt attttctttt tgaataaagt tatagatttc atcaagagtc
    17641 ttttctgtaa ttttctttgc ttcttttata agttcaattt ctttttcatc ttttatactt
    17701 cttaatgaaa taaagatatt ttcagcaggt acaaattgtc tttttgttct ttttgaaatt
    17761 tttaagtatg ttaaataaga aatttcagtt gatataaaaa atgttttttt gaaatcgtca
    17821 agaaaattta ttaaatcatc ttcattatat ttatgtattt gtatattttt attactatca
    17881 attgcctgat aaaaacataa ttcaggaaca aataagtcaa cttttttttc tgttattgca
    17941 agaattcctt caacttctga aattcccgtc aggtaaaaaa tgtttgatgg attaaatgtc
    18001 aaaaaacacc ctcccttctt ttttttgaga aattccctga ctttttctat tctttcacaa
    18061 tttacttcta aagaaatctc tttattcaaa attgctcctt atttataaat cagggtttgt
    18121 aaaaagaata aagaaaatgg agaagtcctt tctgacaatg aagtcggttt tctgcttggg
    18181 tataaacaat tgagtttttc ccatctataa tttcatcagt tacttcccaa ttacgatgag
    18241 caggaagaca atgcataaaa ataaagtcag tttttatttt tgcaatgagt ttactattaa
    18301 cctggtattt tttaaaaatt tccagtcgtt tttccttttc tttttcatct cccatactta
    18361 tccatgtgtc agtatataaa atatcagtat cttcaatgaa atcatatgga ttgtttgaaa
    18421 ttcttatttt gttttttacc atttttaaaa ttttttcaga aggcatataa tcatcaggac
    18481 aaataactct tacttccact ccaagtaaat caccaccaag aattaatgaa ttacacacat
    18541 tatttccatc tccaatgtaa gtaagtttta gtcctgaaag tttcccgaat ttttcaaata
    18601 ttgtaaagta atcagataaa atctggcagg gatgaagaag gtcagttaaa gcatttataa
    18661 ccggaaaatt acaccatttt gcaaattctt caacttcact atgactataa gttcttataa
    18721 caagcccatc taaatattta gacaggacat ttgaggtgtc ttttattgtt tctccgcgtg
    18781 atatttgaat gttgctttct tctaaatata ttgtattacc acctaaggag caaattgcaa
    18841 cttcaaaaga tgttcttgtt cttgttgagg gcttattaaa aattagtcca atagttctgc
    18901 ctcttaaaat attttctatt tctctccttg cccatttact tttatacatt ttagataggt
    18961 caaatatttt aaaaatttct tctttactta aatccaatat acttaaaaaa tcccttttcc
    19021 ccatttgttt ttaatccttc atttcagaaa aacattcatc cataatttca actgccctgt
    19081 caatttcttt tttctttaca gtcaaagaag gcataattct tataacattt ccatgtgtgc
    19141 aattaagaag tagtttttta tttagacata acttgtaaaa ttgtctacca tccatttcaa
    19201 gttcaatagc aagcataagt ccaatgcctt tgactttttt tatacaagta tatttttctt
    19261 tcaatttttc aagtttattt ttcaaatatt tcccatattt atttactctt tcaagaatat
    19321 ctttttcttt tattgttctt ataactgaaa ttgcagatgc acaggccaag ggactgcctc
    19381 caaatgttga ggcatgtgtt ccggggacca ataagtcagc aatttccctt tttgctatca
    19441 tggctccaat tggaaagcca ccacctaagg tctttgcaag ggtcattata tctggttgaa
    19501 ttccaaaatg ctggtaagca aaaaattttc cagtttttcc aaaaccagtt tgtacttcat
    19561 caaatacaag taatacatct ttttctttac acaattgata aacattttca agatattctt
    19621 tatcaacaac atttattcca ccttcaccct gaataatttc aataaaaata gatacagttt
    19681 tatcatcaat tttttctttt agtgcatgga aatcattaaa agggacataa ctaaatccct
    19741 caggaagtgg tttaaatggt tcattatata tcttttgtcc cgttaatgtc actgttgcaa
    19801 gagttcttcc atgaaaagaa ttttccattg aaataatttt gtatttacct ttttcttttc
    19861 catatagccg ggaaagtttt attgcacctt catttgcttc tgccccacta ttacagaaaa
    19921 aaagtttccc atcaaaagaa atatctacta tttcttttgc aagaattgcc tgccagggat
    19981 gataataatt atttggaaga tgaatgagtt tttttgcctg ttttgttata ctttttacaa
    20041 cttctggata acaatgtcca agtccactca cagcccatcc agggaaaaaa tcaagatatt
    20101 tttctccgtt tatatcccat aaccaactac catatcccct gacaaataca acatcttctc
    20161 tggaataaca acctattaca taatgttcat aaagttgctt aatctggttt ttctccatct
    20221 tttataatct ccgttccaac tcctgctttt gtaaatacct ctaaaagtat tgaatgtgaa
    20281 atatttccgc ttataatatg aaccttttta actccatttt ttattgcact tatagaagat
    20341 attgctttag gtatcattcc ttcctttatg acttttttct ctatcaattc attcccttct
    20401 gaaagttcaa gaattgatat aagtgtttct gatttttctg ggtgcctcat tattcctaaa
    20461 acatctgtaa gaaaaataag tttttccgct tttaaagact ctgcaattga tgaagcaaca
    20521 gaatcagcat ttacatttaa aagaactcca tctttgtcat aaaaaagagg ggcagcaaca
    20581 agaactttat atttttctaa tttattttta atatattcat tatttacttg gtcaacctgc
    20641 ccgacaaaac caaggtcaac tacttctgca cctctttgat aatggatttt ttttgcaata
    20701 acatactttt cttctggtaa aattgaggaa gcacttgcct gaaatttttc taccagttca
    20761 tcaacaatac catctctgac cctataaaga acatcctgga ctatttgaag tgtatttttg
    20821 tcagtaactc tcaatccatc tacaaatgtt actttactgc cttttttttc aatttcttct
    20881 gtaataaaag gaccccctcc acatataaga ataacattta ttccaacaac ttcaagaaaa
    20941 gcaatatctt gcaaaatatc ttttcttgcc tgttctattt ttaaaatact tcccccaact
    21001 ttaattacaa atatttttcc tttgtaattt ttaatatatg gataactttc aagtaaaaca
    21061 tccgctttct ttaatatctc atcgtccact ttttaccctc cattttataa aattgaaaat
    21121 tttttgtttt gtatttgagt tttttccatt tacttaataa gtattattat aacccttttc
    21181 aagagaaata ttctaccaca tttttaaaga atttaaatcc ataagtatat ttattttttg
    21241 aagaatttgt ccaatcagga agatgatatt ttaaaacaca tctttctgga tgtggcatca
    21301 tgcctaatac aagcccagtt tgattacata tacctgcaat attttcaacc gacccattgg
    21361 gattatatgg ataagaagaa tactccccat ttttatcaca atatctaaat aaaacaagtt
    21421 ttttttcatt tattttcttt aatatttctt tgttttcagg aataaatttt ccttctgcat
    21481 gtgcaacagg taattttatt atttcaggca atcccttaaa aaaatggcaa acactatttt
    21541 caagtttaag gtaaatccat ctgtcttcaa actttgatga gttattccat gtaagtgtga
    21601 ctgattgttt tcctatccat ggtaatattc cactttttac aagaacttgg aatccattac
    21661 aaatcccaat tataactttc ttatcttcaa taaatttttg catttcatcc cataatttga
    21721 attttatttg gtttgcaagg actttccctg ctgaaatatc atctccataa gcaaaaccac
    21781 caggaataat aagaatctga tatttttcta ataatttttt attctcaatt attctattta
    21841 tatgtattct ttcaggaacc ccaccagcaa gtttaaaagc ccactctgtc tctaaatcgc
    21901 aatttgtccc tgcaactctt aaaattaaaa catttacttt caccatttaa ctccacttct
    21961 ccatttatta gagagtaatt caatatccaa tgaaaaaagt tctttttccc cagaataaaa
    22021 ttttaagtaa ttacttttat taactctacc aatacatgaa aattcacttc ctttcatagt
    22081 ttcttcaaat aattttttat ctttttcctc tatttcaaca acaaatcttc cctgtgtctc
    22141 agaaaaaaga tatgtatcaa tttttctgtc ttttgaagga attttagaaa gataaatttc
    22201 tgctcctttt cttccaccaa ttgccatttc tgaaatacat gttgaaaaac caccatcaga
    22261 acaatcatga catgctctaa ttaaattttt atttattgcc ttataaattt tttttattaa
    22321 ttttgttgtt tttgctggtt ctgggacagg aacccgccct ccattaaaat tatatgtcct
    22381 gaaaaattga cttcctccca tttctctttt tgtttctccg catatataaa ttaaattttc
    22441 ctctttttta aggtcagatg aaataacatt atcaatattt tcaataatac ccaagcccga
    22501 tattaaaagt gtccctggaa ttgaaatttt tcttcttttt tcatcaataa aataattatt
    22561 taaactatct ttaccagaaa taaaaggaac tttatatttt aatgcaaaat ccttacagcc
    22621 gttaacacat ctgacgagtg aacctaattg ttttttatct ccaatatctc cccagcagaa
    22681 attatcaagt ataaaaattt tttcaggatt tgtcccaaca caaactaaat ttctaattgt
    22741 ttcttctata caacagccag ccatataata agggtcaata tctccataaa atgggtttat
    22801 tccacatcca actgcaatac cataattttt atcataatga actttaagaa caactgcatc
    22861 agatggacca ttttgtccta taagtggttt taaaatagtt cttgactgga cttcatggtc
    22921 atattgtctt attacttctt cctttgaaga aattacagga cttgaaataa cttttaaaat
    22981 accttctttt aaatcaattt tctttttaca tttttctttt atttttttta tttcaaattt
    23041 actttccaat gatttttttt gcagtccttt atgcaaaaat ttcatatcaa tatcacaaat
    23101 aatttcatct ctaaaatgaa ttattaactt tcctgttttt tcaaattttc caataacaga
    23161 gacatcaaca tcttctgaag aaaaaagttc tattaattcc tttaatttat tttcagggac
    23221 agataaaacc attctttcct gtgattcaga aatccatatt tcccagccat taagtccttc
    23281 atattttaaa ggaactttat ctaaataaac tactgctcct accttttctc ccatttcacc
    23341 aatggcagat gaaagccctc ctgctccaca gtcagttatt gagttataca tatctttatc
    23401 tctggcaaca agaagacaat caagaacttt tttctcaatt attgggttac ctatttgaac
    23461 aacacttgtg ggaatatctt tatctaaact tgttgaagaa aatgtcgctc cgtgaatacc
    23521 atcccttcct gtttttcctc cgacaactac aatataatca ttttcaacat ttcttttttc
    23581 aactttatca attggaatta aaccaactgt tccacaatag acaagacaat tatttgtata
    23641 tctctcatca aaaataatag caccgtttcc tgttggaatt cccattctat tgccatagtc
    23701 cctcacccca cttacaactc ctttaaaaat tctttttgga tgcaacactc cttctgaaag
    23761 tttttcaaaa ggtgtgtcca tattaccaaa acaaaaaata tccgtattta atattggttt
    23821 tgctcctaaa cctgctccaa gaatatctct tattactcct cctattcctg ttcctgctcc
    23881 tccatatggt tcaagtgctg atgggtgatt atgagtttca accttaaaag caaccccata
    23941 tttttcatct aattttataa taccggcatt gtctttaaaa acagaaatgc ataatggact
    24001 atttaatttt tctgtaactt tcataatttc ttttaataaa acaacatttt tttccttttt
    24061 tttctttcct tctttttcct gatatttaat gtttgcttta aatgttttat gataacaatg
    24121 ctctgaccat gtttgggcaa ttgtttccaa ttcacaatca ctcggatttc tcccaattgt
    24181 tttaaaatac ctctctattg ctctcatttc atctatatca agagaaagaa gtcccttttt
    24241 acttatttca agcaattgtc tatcaccttt tccaattatt tcaatttctt tagcatattt
    24301 catctaattt cctcttttta ttttgtagtc atgtattaaa ggatttgcaa gtaatcttac
    24361 gcatattttt ttaatctctg tttgttttaa actgcaataa atataatatt ttttccctgt
    24421 tgatatattt atatcctttt ttaacccata atcagaaatt gctttctttg ttgtttctgc
    24481 taccatatcg gtaacacctt ttttatacca tatctcaacg aggtataaat ttttttcttt
    24541 tgttgtctct atatttatag aatatttttg agagacccta tcaagaagta tgttttcagc
    24601 aatttctttt atttctttta tttctacatt accgtcaaat gcataaaggt cagaaacatt
    24661 tacttttttt acatcatata tccccatttc ttcaacccct ttttttgtct catttccaaa
    24721 agcatcaaat acattttctt tgtaaaaaat ctcaataata tatttcattt ttcccttcct
    24781 ctttcaattc cttttataaa acaataaaat gaactaccta aaacatcaag gttatatcca
    24841 ccttcaaata taataaataa attcatacct gttttaccaa tttcttttcc catcagataa
    24901 tagtccgact cttcaagatt aagagaaaga agggggtctt ttttatgggt atcaaagcca
    24961 agagaaatac caataatatc tggagaaaat tcttttatta tatttaatgt attataaaat
    25021 ttttcaagat attttttatt tcctgttcca ggggcaattg ggaaattata acagttttca
    25081 aaagagaaat ttcctgttcc cgggaaagcg ggaaattgat gaattgaaac ataaataaca
    25141 ttttttccca tttttaaaat atcctctgtc ccatttccat gatgaccatc aatatcaagg
    25201 actgcaactt ttttcccttc atttatgatt tttttaactg ctactgctat accattaaaa
    25261 taacaaaaac caccaagaaa gtgttttcct gcatgatgac ctggtggacg cgaaagagaa
    25321 atacctatat ttgatgtttc taaggatatt tcacttgctt taactcctgc acaaacagaa
    25381 aggaatgcat atttgtaaat atccaggata acaggtgtat ccatagcaga aaaattatta
    25441 ttcttaacat tttcaacaaa ttcccgacta tgtgcaagaa acaataagtc ctcattataa
    25501 aggtcaactc caacaaaatt atttattcct tttttaatta aaaaattgta tataactttt
    25561 atcctttcag gacattcagg atgacctttt tcgtaatacc gaagaaaatc aggtgaataa
    25621 acaatttcaa atttcatagt cctcaaacat atatattatt tcttccatat tggaaaatct
    25681 ctcatcgggg ttttctcttg ttgagatatt tattatatat ctaaatgtat ctggtattgg
    25741 gaaattaagt tcattatata atttttcaac tgttttccca aaagagtata tatcgcttct
    25801 tatacttgct tttctccctt taataatttc aggtgctgta taatcaagtg taccaccttc
    25861 tcttttttct ttataaaaaa atgtgttttt tactgataaa ccaaaatcag ttatttttac
    25921 tttttcaaga gagaaattta caagaatatt ggaaggatta aaatccttat ggataattct
    25981 attttggtga atgaaagata atccctttgc tgattttaaa ataatttctt ctaaaattga
    26041 aggagagaaa atttctcttc tggtatgtat acattttaga aggtctgcgt caagaaactc
    26101 cataaaaata aaaaaagccc cttcatcttt tttaaaatca taaaccttca caatatttgg
    26161 atgtataagt tttttagcga tttttgcttc tcttataaac cttcttattt gatatctatc
    26221 aagacgtctt ggatgcaaca ctttaattgc aataactggg tcatattctg gttgtgggtc
    26281 tatacttctt gccttataaa cagtagaaaa cccacctgct cctaacatct cttcaagaat
    26341 ataattttca attttatagc cagttcttac ttttatttct tccattttta aggttaattt
    26401 aaggtcatta aaatgaagtt gtattatttt accatcatca ttaattattt ttcaacatct
    26461 ttatattgag atatattaaa tttaattggt tatttgaaaa aaataatatt tgaatttttc
    26521 tattggacaa aattatggaa aatataagtt tttgatttgt aaatattttt taacttcgta
    26581 agtaaaatta caaaatatct aaatcagtgt ttctataacc aattgcttct gaaatatgag
    26641 aagcatttat tatttctgat gcatcaaggt ctgcaattgt tcttgctact tttcttattt
    26701 tatcatatga ccttgctgaa aaattaactt ttctcattgt ttcccttaaa atttcttcac
    26761 cttctttttc tataatacaa tactttttta tctgctggga attcatatat gcattaaaat
    26821 aaatcccttt atttttaaat ctctcccttt gtatttgtct tgcttttaca actctatttc
    26881 ttatttcctc ggatgattct tcttctctat cttcaattaa aacatctgct ggaacagttg
    26941 ttacttctat gtgtatatca attctatcaa gcaggggtcc tgatattttc tttctgtatt
    27001 ttaatatctg tgaaagtgtg caattacagg tatgatgaat atctccatac catccacatg
    27061 gacagggatt catagcagca acaaataaaa atcttgcagg aaaatcaact ctcccttttg
    27121 ctcttgaaac ggagacatta ccattttcaa gtggttgtct taatgcttca agaacatttc
    27181 ttttaaattc aggcagttcg tcaagaaata acactccatt atgagataga gatatttctc
    27241 ctggttttgg tattgttcct ccacctatta gggcaatatc agaaattgta tgatgtggag
    27301 acctgaaagg tctttcaatt actattggtg tttttaaaat acctgcaact gaatgaattt
    27361 ttgtaatttc aagtgattct gtcagagtta aggggggtaa aattgttgga attcttgaag
    27421 caatcatact tttccctgct ccgggacttc caaacataag gatattatga aaaccactaa
    27481 cagcaatttc aacagccctt ttaacatatt tctggtcttt tacttcttta aaatcaacag
    27541 aatatttatc tttttcttca aaaaggtcat taaaatttac tttaaatggt ttaatttgag
    27601 tgtccccttt taaaaaagag gatgcttcat ttaatgtctt aactggataa atttcaactc
    27661 cttcaactac tcctccttca aatttattct cgtcaggaag tataatttta tcgcaaccat
    27721 cttcttttaa ttttaaaacc attggtaaaa ttcccttgac aggtcttaca tcaccattta
    27781 aagcaagttc tccaacaatg tagtaatttg aaagagtatc ttttgggata tatccaagtg
    27841 cagataaaat tcctattgaa attggtaggt caaaataaga accctctttt tttatatcag
    27901 caggggcaag atttataaca atttttctgt ttgggaattc atatccagag ttttttattg
    27961 ctggttttat tctatctcta ctttctttta ctgcctggtc aggaagtcca acaacagata
    28021 taccaggtat acctctagca atatcaactt caacctctat ggggaaacct tcaatacccc
    28081 atattgttat agacctgact tttgaaagca tagtaataga gaagaggggg ttttgccccc
    28141 ctcttatttt tttattcaac cttaattttc acttcttttt tcttttcttc tggtgatttt
    28201 gggagagtaa gttgaagaac tccatcctta tatgatgctt ttactttatc tttatcaatt
    28261 tgagcaggaa gagttaaact tctggaaaaa gttccactcc ttatttcttt tctataaaaa
    28321 ttctcctttt taacttcttt ttcctcctgg ctctttcctg atatagtcag aatatcacca
    28381 ttaagagaaa cagaaatatc ttcttttttt acacccggaa tttctgcttt tactatgatg
    28441 ttatctcctt cttcataaac atcaatcgct gggactcttc ctccctccca taaatcaagg
    28501 tctctattaa aaattctatc aaccaatcta tcaaaatcat ctcttacatc cataatttct
    28561 ttaaaaggtg accattttgc taattttgcc atatctcatc acctcccttt tttttatttt
    28621 tagatgcaaa aaaaaggtgc aggtttcatt ttttaaattt tatttctatt tgatgatata
    28681 ttcttttact tattactttt ggaacacttt tctttttata aatcttttta cttaaaaaaa
    28741 ttgttttctc cagtgattca aataaagaaa ggcatttctc acatatttta atatgttctt
    28801 taattaagtc attaatgggt ttttcaagtt ctccatcaat atattcagaa ataagttttt
    28861 caatatcttt acatttcatt ttaattccca tgttttttaa tatattcaga aattttttct
    28921 cttaaaaaca tcctgcttct atgaattctt gtttttactg ctggtattga taaagaaaga
    28981 atttttgcaa cttctgatat aggtagttct tcaatatcat gtaaaataaa aactgatttg
    29041 tattttggtg gaagtgaatc aattgcctcc tgaagttgtt gctttaattc attgctttta
    29101 aaatataacg aaggattttc ataccaatca gaaatttcta ttttgtaatc tttatctcca
    29161 attttcttta tttcatctat tgatatgttt ttattttttt cttttctcaa tttcatcaag
    29221 gcattatttg tagcaattct atatagccat gttgaaaaag aagacctgcc ttcaaattta
    29281 tcaagatttt catatgcttt tatatatgta tcctgcataa ggtctgccgc atcctcttta
    29341 tttccaagta atctaaagcc caaattataa atattagttt gtgttctttt catcagttct
    29401 tcaaatgctt ttgtatcacc tttttttgct ctttcaataa gttgttgttc actcatttta
    29461 tttctggtaa atttcaacta ttttatttct gcttgttttt cctgttatta tttctatatt
    29521 ttgtttcggc acattaaaat atttagaaag aatttcaatc aatttcttat tcgctttccc
    29581 tttttctgcc ctttctttga cacaaacaga aaaaattcct tctttttcct caattttatc
    29641 ctgttttgcg tctgttttca ctttaattgt aattttcact taataatagt atcataaaaa
    29701 agttttttaa aaaaatttaa tgtatttaag ggttgacttt tattaaagga taggactata
    29761 attatgtaaa aggaggcaaa atgaaaaaaa ttattatttt tttgctggta gttctttttg
    29821 ctggatgtgc ggttgtcaat gtgtatgtga cttttcccga agagaagatt aagaaagcag
    29881 cagaagatat tcttgctcca gcaccatctt ctccatctgg atttctaaaa tataatttta
    29941 ctaaaaatct ttatgcagat gaaaaagttg ttgttggaaa agaacttaaa actgattctc
    30001 cagttataag acaggcacag gaaaaaataa atagttggag aagtaaatta gatgaattta
    30061 aaaaagatgg atatgttgga gaaacaaacg attttaaagt tgttataaaa caggtaccag
    30121 atgataagca gaaagaaact gaattaagag aacttgtaaa aaatgaaaat acacaaagag
    30181 atataataat aagtgaactt atgaaaatta acaatgctcc tgccaaagaa gaaaaaaaat
    30241 ttagagagat atttgcagaa acatcaataa aatattctcc ctctggaaca tggatacagg
    30301 atgcaaaggg gaattggaaa agaaaatgaa aaaattgttg ttaaatgggt gtgaagcagc
    30361 aggggaaggg gcaatttctg ctggctgtaa attttatgct ggatatccta ttactccaca
    30421 aaatagattt ccagagtata tgagttatag gttaccagaa gaaggtggaa tttttataca
    30481 atcagaaagt gaaattagtg caataaatat ggtctttggg gcttctgctg ctggggttag
    30541 agcgatgaca tcttcatctt ctcctggtat ttccttaaaa caagagggaa tttcatatct
    30601 tgctggttgc gaattacctg ctgttataat aaatatgaat aggggtggtc caggtcttgg
    30661 ggatgttacc ccatctcaat cagattattt tcaagcaaca agaggtggtg gtcatggtga
    30721 ttatagaaca atagtgcttg ctccatatag tgttcaggaa atttacgatt ttacctatct
    30781 tgcttttgac cttgctgata aatatagaaa tccagttctt attcttgcgg atggtattat
    30841 tgctcaggtt atggaatcag ttaatttaaa accaaaaaaa aagaaattac ctgaaaaaaa
    30901 atgggcttta actggatgta aagggagaaa accaaattca ataaaatcac tttttttaac
    30961 tcctggggta cttgaaacac ataactggaa attagcaaga aaatatgaga gaattgagaa
    31021 aaaagaaata aggtatgagc aatatttttc tgatgatgca gaacttggaa ttgtcagtta
    31081 tggaacatgt gcaagaattg ttcttgaatc ccttaaaaag gcaagaaaaa atggcataaa
    31141 ggcatctctt tttagaccaa ttactttatg gccatttcca tataggcaat tatctgattt
    31201 tataaaaagc gtaaaaaaaa ttcttgttat agaaatgaat ttaggacaaa tgcttgatga
    31261 tgttaaaata tcaaatacaa gtaataagag aattttcttt tatggaagac ctggtggggg
    31321 agttccaaca ccagaagaga tatttaaaga gataaaaaaa atatgaaagt tatttataaa
    31381 aaaccagaaa gtataacaga taatactaca atttattgtc ctggatgtgg gcatggaatt
    31441 gttcataaaa ttattgcaga agttattgac caactggata taagagaaaa aacaatatct
    31501 attgctcctg ttggctgtgc agtactatct tataactatt ttaattttga tgtaacagag
    31561 gcagcacacg gtagatgtcc tgctgttgca actgctataa aaagagttta tcctgatagg
    31621 attgttttta catatcaagg agatggagac cttgctgcta ttgggactgc tgaaattata
    31681 cattgtgctg caagaggaga aaatataaca gtaattttta taaataatgc tgtttatgga
    31741 atgacaaaag ggcagatggc tccaactaca ttaccagggc agaaaacaac aacaactccc
    31801 tatggaagag acacatcata tcatggttat cctataagaa tgtgcgaact cctttcaaca
    31861 ttagctggtc cttcttatat tgaaagaact acaatagaaa atgtacctga aattataaaa
    31921 acaaaaaggg caattcaaaa agcatttaca taccaagtag aaaataaggg attttctttt
    31981 gttgaagttc tttcaacctg ccctacaaac tggaaaatga ctccttctga agcaatgaaa
    32041 tgggtagggg atacaatgaa aaactatttt attcctggtg tttataannn nnnnnnnnnn
    32101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32161 nnnnnnnnnn nnnnnnnnnn nnnnnnnaaa cataatgttc tttttgcctt tttcaatact
    32221 actacttttg atttttattt tacttctgcc ttttttcttt ttacttgttc aggtagaaat
    32281 aataaaaatt gctttaagta aattaggact ttctccatat atggctcttt taatatttct
    32341 tttatctcta attggcagta ccataaatat ccctcttttt tccagagaag ttacaggagt
    32401 agttcctaat agagaaatgt tctttatttt caatccattt ggtatgccag aagttcagac
    32461 aaaacaaata atagcaataa atataggagg agcaataatt ccactttttc tttgtatata
    32521 tcttctacca aaggcaccac cacttcccac tttatttgcg actttaattt caattattgt
    32581 ttgttttaaa atatcaaaaa ttgttcctgg aatgggaatt gttatacctt ctttaattcc
    32641 accaataata gcggttctct gtgcttatat tttttctcct ggaaataaaa tcccagttgc
    32701 gtatatttca ggagttcttg gtgttttaat aggagcagac cttctcaacc taactaattt
    32761 gggtaattat cctggaatta tgagtattgg tggagcaggt gtttatgatg gtatatttct
    32821 tgtaggtatc atttctgcac tacttgcata actaatagcc gttctatttt atcaaaccac
    32881 tgcttataat attcattttt tatgatatta gatattagtt agatacttta tatcataaga
    32941 ttttattgac ccctgttcat atccccaaca tggaagcaca cagatacaaa attttaatac
    33001 tcacactcaa aactcaaaaa taaaaatctt ttatttttat tgcctgctac tctttacttt
    33061 tcagtcaatg caattaagta agtacacact gactgagtat ttcccttatt tcatattcaa
    33121 tagaaaaatg actcattttg aaataggtat ttccagggca agaagaggtg gatattgctg
    33181 gcatccaaaa atatcctttt cacctggtga accactcggt ctattacgca aaaaagtaaa
    33241 tttgatagca taagcaggat caaactcaac aaaatctgtt atgcgaccaa tgtcaatatt
    33301 ataaagtttt gatatgttct ccctacttaa cacaccactc tttctgacca tttcgtagtt
    33361 atcagcgcta ttaaaaatta tatcaaaggt aattttatca gtacctgcat ttttgctacg
    33421 aattgtcttt gctaaatcac ttaatttaac agttttcata aaattctcct tatattattc
    33481 atcggaatag gtaaatttaa ctttaaaaag gtccattgga ttatttaccg ggatagtatg
    33541 attcattgtc cactcatagg caacaccgtc atatattgtt tcatgtgtta taaatgcagc
    33601 actaccagcc gttcctttta catccggtaa tctggcataa aaaagttgca tccatccaca
    33661 aaaagtgatt tcttttgcca attcctttgt tgctgctact ccctctacta ctatacacaa
    33721 ctcatgtgat ctgacttccc ggactggttc ccactcttcc ataactccgt ttttcccata
    33781 gatatgaaaa taaagctcat atttttctcc tggaaattgt tcctgcactt gctttctgca
    33841 ccaagcaata accctgtcaa tatttttgat tgaatatgga tcccgaatac ctgctattcc
    33901 aatatacctt tcaccaactt tacctgctcc ttcaatttta actcttattt cttctgctgg
    33961 aataaatttc ggaccagtaa tacaacaagt ttttttatca tactgaacat attgacaact
    34021 actcatatcc aacataccac ccacaaaata ttcaaaatag ggattagacc gttcatacat
    34081 tgcatgacct gccactgatg caactgtaca atattgttct ggatgcatcg ccttgacctt
    34141 aatgtcttgc tcagtaataa ttccaataat tgtttccttt gccccgtaag gttcagcaca
    34201 gaaagaagca cactccatta ttttaccaag gcaatatgca agtccttctg gaaatccttc
    34261 tcttattgct ggtgcagcaa aaatagcaac atcacctgaa cggcctccaa tgataacatc
    34321 aacacccata tccaatgcct tgtgataggg atgaatacca gcaacagcaa caatattatt
    34381 cgtcttatca atgtctccca tggttaaatt ttttctacca tccagtcctt taatttcttt
    34441 cccctttttt agcaaatttt taagataatt ttttgttaaa tcggaataga aatatcctat
    34501 cttaaatttt ggtagtttat gttttaaagc aatctcctta atatatttta tatacctatc
    34561 aaccataact tttgaaccac aatctccagc cgagccaata atcattggga tatttttctt
    34621 ccttgattca accaacatta gttctaaatc atgtttctgg tttctttctg aactgaaact
    34681 tttgttggct ccaagtgcac ccggacccac gtcatcactt cctgaatcag caataatata
    34741 gtcaggattt tcttctatcc ctgcataaaa acttgcttct ttgatggggg cagttcccaa
    34801 atgcccagta gaacataaaa acttcagcat tttctttcta tccatatcgc tccttcgttt
    34861 ttattgattg gttgtaaaat ttttatctaa aaaaatttta accatacttt taaaaatatc
    34921 aaatttttgt atgagttggg tacatcggta acactttttt attcaaattt tgttgttttt
    34981 tcatctcaat aaattccatt ggtgttaaca tatcaagaga acgatgtggt ctattaaaat
    35041 tatactctac taaccagtca gttaaaagac cattaaatat agaaaggtca tcaatataat
    35101 tgcccatctg caaaaactct tcttctaatg cctattaaat ttctctccat ttaaggaaga
    35161 gaagtggcgg gatggggatt tgaaccccag acttaccggg tatgagccgg ttgctctacc
    35221 actgagctac cccgcctttt ttaataaata ttctgctaat tttctgaaag caatacttct
    35281 atggctgatt ttattctttt cttcaactga aagttgtgca aatgtttttc caatttcagg
    35341 aatttcaaat acagggtcat atccaaaccc attttctcct cttggctgga atgttataat
    35401 accttcaacc tcacctttaa aaaattttct cttatggtct tctataagac agataacagt
    35461 gataaattta ccttttctat cttctttatt ttttatattt cccatcattt tcaacaattt
    35521 ctcaatattt tttctgtcat ctctataagt agaagaaaat ctcgctgact taacaccggg
    35581 cagtcctcct aatttttcaa ccataagtcc tgaatcttca cctacaacag gtatattatt
    35641 tttaaggaaa tttttaagat aatttgcttt tataaaagca ttttcttcaa atgtatttcc
    35701 tgtttcttct gggaacatta tatcctcagg acattttata acttcaattc ctgtatcttt
    35761 taaaatatcc tgaatttctt ttattttatc tctgttttta gttgccaagt aaaaattcaa
    35821 tttcatcttt taatattttc ttttcaatct caattatttc ttttatacct ttttcaccaa
    35881 gggtaataag gttgtcaagt tgttcttttg agaaagaata tccttctcct gttccctgaa
    35941 tttcaacaaa ttcattactt cctgtcataa taagattcat atcaacactc gcaacagagt
    36001 cctctgaata atcaaggtct aaaagataat ttccataaac tattccggcg cttatgcctg
    36061 cgagatagtc ctttataatt ggtaatcgta aatgatttgt tttttttaag ttattcaaag
    36121 caagaacaag tgaaataaat ccacctgtga ccgatgctac ccttgttcct ccatctgcct
    36181 gtaaaacatc acaatcaatc cagattgttc tctcatcaag atttttcaaa ttgataattc
    36241 cccttaaaga cctgcctatc atcctttgaa tttcttgact tctaccagaa aagtttgaac
    36301 ttcttgggac tctttcactt gttgaagatg ggagtaaaga atattctgct gtaacccatc
    36361 catttccaga gtccttaagc caggcaggta ctttttcttc aatatttgca gtacataaaa
    36421 ctcttgtatc tccaatttct ataagacagg aaccaccggc atatttaata aaattttttg
    36481 taattttaat ttctcttatt tcatcatttt ttctattacc ttttcttttt atctccattg
    36541 ggaatacctc tctttccaag tattttgttc aaaaaatata gttggcagaa ttaaaaaaga
    36601 aaaagaaaaa ttatctctat ctctactcat tttaaaatca atagttaaac aatgcaattt
    36661 tctccatatt tcatatgact gaacaggaat attttctgtc tcaaaatcat aataaatacc
    36721 aacacgatat ttccagtttt catttatttt tttttcaacc cagttttcaa taccacaaat
    36781 atcattatct tcttcatatc ttgttccaaa agaatattta aaattttctt tctcaaaaat
    36841 aaggtcattt attccaaagg cataacttcc tttattaaaa tcaaaatcgt tttcaccagt
    36901 aattgaaaaa ttattattta ttttaatttc ataatcaatg tatgtattat taaattcatt
    36961 tctattaaag tcataggcat tagaaataga aatattacct gtatattcac tttttttaaa
    37021 taaattccag tcaaaattaa cttccataaa tttcccgttt tccatttctt cataaggact
    37081 taatggaggc aattgaagtg gtttatattt tgtagtcctg ttataaaaat atattgaagg
    37141 attaaagatt gtctcttttt cgttttgttt ttttatcata gcagttgaaa gtttaatacc
    37201 aacttctgaa atataattta atttgtcatc ggaagagtta ttatagttaa ttccagaaaa
    37261 agatataaag ggagaaagag taaaaaagcc aatattattt ttattttcaa acattatttt
    37321 ttctactgct ctaaaatagt tatcattatt ataataaaaa tttgtaaggg aaaaattatt
    37381 tgagaaaaaa atgggtgtat tggaaatttg tttataagga gtaaaaattt gaatttctgg
    37441 taatttttca acacttaaaa tttcttctct ggcattttct cttatgttga aattaaaaaa
    37501 agtattgtta tagttatgag ttattgaaaa gtagttatat gtttttgatt ttgaataata
    37561 tttttcagaa aagaaatcat taaagaaatc attatcatac atccatctcc agtcaaaaat
    37621 tatatcccca ctgccttgtg gtttttcata atttttttgg aagttcccta ttacccctgg
    37681 ctgaacttta tcttcatcat actttttcat tcccaatgcc tggaaattaa aattattttc
    37741 ttcggatttt gcatttatcc cagtacctat tcccttactt ccaatatgaa aaaattcttt
    37801 taagtcaata tcattttttt catttttctg gaaaaaatca acttctaaat ttcttcctat
    37861 tcttgtttta taactaattt ttgcctgtgt tgatggctgt ttttctttta tatgatattt
    37921 gaattttggg aaataaaata cattataatt tccagcaata actttcatct tttctatttt
    37981 aatgtaatca ttttcaacat aagttattct atcagcacca aatctgtaat gagggtggtc
    38041 taaatcacat gtagttatat atccattttc tataattaat gagttgcctt ttttttcaag
    38101 atttttccct tttccataaa aaggtggaga agaaaatgat gcacctgtta aaatccagta
    38161 atccttatct aaataatatt ttgcattttc tacatcaaat tcccccattt ctgcctttac
    38221 tttcacattt ccattacaag aaatttcacc tgttatttta ttaaactctg cttgctgaca
    38281 aaaaagatgg acatctttat aaattacttc aacatttcca attaaagaaa taccagacaa
    38341 ttctccattt tcagtgaaat taacattcat ttcatctgct gtataataaa ttgtttcaat
    38401 ttcagaagaa tataaaaaag aagagaagaa acaaagtaat ataataaatt taaattttga
    38461 taccatattt tgtccagaaa tttttcaggt catcccagga aataattcct gaagttgccc
    38521 ctactgatgt tgttattttt gctcctacga catttgcaag aagaccacat ttttcaaaat
    38581 tatagttatt cacaattccg gtaataaatc cagcaacata tccatctcct gcgccagttg
    38641 tatcaacaac atctacattt aaagcaggga aataatgtct ttcttttgaa ttaacaatat
    38701 aggacccctt ttctcccatt tttataccaa cattcctaac tccttttgaa agtaaaaaat
    38761 cacctatttc ttctacattt tcctttcctg ctatcacctt tgcttcttca taacttggca
    38821 gaaaataatc tatatatttt aatgttcctt caattaaaga aagatgctca acttctgcat
    38881 tccacacagt atcaagaact gttataagcc ccatttcttt tgcttttttt agtgtttcct
    38941 ctgttggtct gccatcaaac tctggcataa caaaagtccc tgctatatgt aaaatatgaa
    39001 aatttttcat attatcaaag tcaatatctt caattctcaa ttttgaattt gctccaattg
    39061 aatgaagaaa agacctctct ccatcaggat aaactaaaac agatgttcct gaggtattta
    39121 tttcatcagt aattttaatt ccgtctgtat ctataccctc ttcctttaat ttgtttatta
    39181 aaaacttacc taaattatca tttcctactt taccaataat tgctacatta caacctaatt
    39241 ttttcaaatc aatccccgta tttgctgcac aaccgccaat atgtaattct gccctatcta
    39301 caagattcaa tttacccttt tcaggcattt ttctaactgg ttttacaaga aaatcaccta
    39361 ctataattcc aatacataag accttttttc ccatattaac tcctttttta atacaatttt
    39421 ttgtctcgcg aactaataca attttatgta ttttctttgt ttctgtcaat tttaaattga
    39481 cattaaagga aacaaaaatt ataataaagt tattggggga ttagctcaat tgggagagca
    39541 ccaggctggc agtctggggg tcaagggttc aaatccctta tcctccactt tctcttctac
    39601 aagaagggtt tgaacccttg gggggcaaaa gggtcaaacc gactaaagag gcaaagtata
    39661 gcaaagcaga gttggtgcgg caggaacttt gacttctttg tgtaagcaca atcccttatc
    39721 ctccacttta tctttttgga aggttataga atagaagtta aattcttact tttcttttaa
    39781 aattctaata agtatatcag gtctgtttgt taaaattgca tctgccccat tttcaataag
    39841 ttttttcatt tcattttctg tatcagcaaa aaacacatta acaaatatcc cataggaatg
    39901 tgctttatca attaattcct ttgttacttc ataagatggg gtaaaaaatt gtaaaatagg
    39961 gcatttcaaa cgatgggtct cttcaatata ttcaacaggg tttgtctgtg ttctcatatt
    40021 gcaaatacga atttctttac acatttctct tgctaattga atttgacttg catgaattgc
    40081 aagataagaa ttatttattc tttttctttc ttttaaggta ataataacat ttcttgtaat
    40141 aacaggattt ggtagaagat gaagatttaa ttctatatta tcaggtatgg aatctaatgc
    40201 ctcttcaaaa gtagggattg tagtaccttt aaatgcggga ctgaaccagg aacctgcatc
    40261 taaatctttt acttgttcta ttgttaactc ctgtattttc cctgttccat ctgtagttct
    40321 atctacactt tcatcatgaa gaattaccaa agtcccatct tttgtaattt ttacatccat
    40381 ttcaatacca tccacaccaa gttttgctgc ctctttgaag gcaacaactg tattttctgg
    40441 aaattttcca gaaaaacccc tatgtgcaat tacttttact tttttctttg tatttgacat
    40501 aacttttctc ctttattttt ctaattttca gagtttattt tttgttttaa aattgctgca
    40561 cttgtttgta atttctgtaa tctatttgtt agttttttaa tactttcaga atatatatct
    40621 gtccctttct ccaaaagaat tatatttttc cacatataat attgctcagt taagtcatat
    40681 gtagtatctg ctgtaattga taaatatttc catactgccc ctggaacagt tttatcaagt
    40741 tcaagcaatc catttatttg agaaatccca tcttttatta tctcaagtgt ttctataaat
    40801 gagcgggggt ctatttcaga aaggtccaca aaatctttag tttcttttgt ttcttttaaa
    40861 ctttgtatta aagagcaggt atatttatat ttttcaatta attcatctgg cttatcaggt
    40921 aattttttcg caagtttata tatagtttca tatctattct gtaatccttc aatacctatg
    40981 tctataagta aatctccaac cctgtttata cttctttcat atttttctgt tgcttctttt
    41041 tcaatttctt tgaattgttc agtaagtaat tgacttgatt gaataagttt taataatact
    41101 tcacgagtgg aagatatttc tttatttact ttatctaact cctctgccca ttttacacaa
    41161 ttcttttccc atttttcctc ttctgaaatt gaagaagatg aattttgagt ttttgtatct
    41221 tgttgacccg atattgagat atttttaatt ggattaacaa tagcattgtt ttttaatgta
    41281 attaataaat caggtgcctt ataaggattg tttttatact gaataatatt atctctgatt
    41341 tgttgtagtt tttttattgc ttcattctga gattttattg ctttttcttt tatttcttct
    41401 gctttatctg gtgaagtggt aattattgtt tctgcatatt ggaggtcttt attaacagaa
    41461 cttatagttt caaaacattc atttatctga gaaattgctt ccttggtggt tatagcatat
    41521 ggggctgctt catttggcat aggtatattg ggaattggtg gtaaatttga aaggtcaatt
    41581 tctcctgtca tagcacgggc acttctttct gcaaggtttc ttgcatcttc taaattacca
    41641 tttatagcag aatttactgc ctgttctgca agaactacgc ttcctgctaa ttgttttagt
    41701 acatctgaag ttctattaac atcagtttga attttcatcc attcttcacc ttcttcctga
    41761 ccaaaacttg cgcctcctga ctgaattaat atcattgaac ctttaccatt tgtaatttgt
    41821 aatgttattg cccaaaaccc atttacattt ttaggttggt caaaatacat agaagaagtg
    41881 tttagtacat taatagtata attgttttgt ttagcccatc ttgctaaaca tacaagtttt
    41941 gcaacttctc tcattatatg aaattcagga aatatttttg catattcatc ataattcttg
    42001 gttagaaatt ctccatactt tttagccgtc tcattaagaa aattagttgt ttctggtgat
    42061 tggtttttaa atgttctctt taaaaaaccc actattttaa cttcggactt ctcaaacaca
    42121 ataatactcc catccttaga aattttcata tcaacatatc ctggaactaa tgtatgtcca
    42181 cttgttgccc caatatcaaa aggtagttca taatttttct ctttttcttc cttttgtata
    42241 aactccattt cagttaaatg ttctgggaat ttttccttta catctggaaa attattaata
    42301 ttttttaact tataatcggc tttaaataaa atttctccta atagtgaacc tgcctttaca
    42361 tttttaaaga caagttctga ttgtggtata ggtaaattat acaattttga aattaattcc
    42421 ggacctaata ctaatccatt taacattttt tctcccattt tttcagatat aaagaaagta
    42481 aatctcttgc ccatatagtc aataattgta tctactggtt gtctacctga cctaatagaa
    42541 gaaataattt tttgaagttc attttctgga gcatccattt ttctgcacag ttctgatata
    42601 catagaataa tcgcttcttt tcggaattct tctgggaata agattttctt atcaagttca
    42661 tgatatttat catagatatt aagtgctttt gccatatttg aaagtcgttc ctcaggggta
    42721 ttcccagaag taattgcaat taacccattt gctgcttctg gagcacctgc tccttttaat
    42781 aattttgctc ctaactgaaa tagttcggat tctgaaaggt caatttctcc aatagatgct
    42841 ctgtataatc tcttgaactc atcacctgtc attcccacag cagatgcgat attattaaag
    42901 aaacggcgat tatctgtttc taatgttggg tcttttaaaa gaagatttac caacttttgc
    42961 atttcttggt ctgtataggc agggtctttt tccatctttt tcaagtcctg agaaatcaat
    43021 tggtcaactt ttttaagtat ttcatcttcc tgtggagttg gttcaattga aacagatgga
    43081 taaggatttt ccagtaaatc ttttaaaata tcttcatatg gtatatcacc tgttgcataa
    43141 gttgggtcat aactacctat aaaagtaatt atatgtgttt tttggtcaat taaagcatat
    43201 ttaagggtat ttatggcggt aagttcattt ttcattggga aaaataaatt attttttgga
    43261 gaattttcag ccaatgtttg tgaaggaact acaactggtt tgattttcaa aactaattta
    43321 atattattcc gtaagacaac aacaggatat tctgtatcca catttaaact atgcatgatt
    43381 tcaaaaactt cttttacaga tgaaatttgt ctatctgcaa gttcaattat aatatctcca
    43441 ttatttattc ctgcaatttc cgctggagaa tttttaagta cctgctgaac tttggcacct
    43501 ttaatttgtg aattatctgg taagtccgta attataatgc caatccatgg ttgtgtattt
    43561 ttcattgatt ggattctttc cttaacttca ggacttacct gtcgagcact aaggaggtag
    43621 aaattttgac aattattact caatttcatt tgaatttttg atatattttg agaaaaaatt
    43681 ggacttataa aaaaaatatc tataaaaaaa acaaaatata atagtttttt catcatatta
    43741 cctattattt ctaattttga taaattattt aacattttca tttgtaaaaa tttatcctat
    43801 tcatcttttt ttgtcaatta ctgaatcttc atcaaagaat tgtaagatat aaaggaattg
    43861 taaaaagaga aaatatgtga gtaagaaatg ttgttacagc agcatattct gaatcagcat
    43921 tatatgcttc acttaaaaca atgcttgcaa ttgctgttgg cataattgcg acgattgaaa
    43981 gtataagttt tgtttcatat ggaaatccaa gtatgttgaa aagaaatatt gaaatagagg
    44041 gaataaaaaa taatctcaaa aaaattagaa tagtattttt taaattaaat aattcttcct
    44101 tttttatatc tcccattctg catccagcaa taaataaagc aagtggagat gttatttttc
    44161 caagaaggtc agttgcaatt aaaattgaag atgaaaatgt ttttaaatat tgatttgaaa
    44221 ttgtaattat acttctatcc cttataaaaa ttaaaacaac agaaattaaa attgcaatca
    44281 taggaacact caataaattt ttaaaagagc ctttccttat tttatttcct gttaaagcaa
    44341 taattccaaa tgtccagaca gaaatttctg aaccaaaagt tgaaagtatt aatttgggta
    44401 tcttttgttc tcctaaaagc acaataataa tagtaattgg taaaaaagaa taattattga
    44461 ttgcacattg aaatctgaaa gtattttttt ctttttcagt tccgaattct aaaaattttg
    44521 aaacaaaaat acctgttaaa taaccaataa acataagtat aaaagtacct aatggtaatt
    44581 gccatccact gataatgtca tttgtagtaa aattatttag aaatgatgag aaaataagag
    44641 atggataaaa aaaagtagtt atagaaattg acatctcttt tattgtagat agagatatat
    44701 attttctttt tctcgcaaaa aagccaaaaa gaattatcaa aaaaacaggt agaattttaa
    44761 ggaatatcat ttttttaaaa tctctttaat gaaataaata aatacaatta ttgagtgaat
    44821 taaagtatcg taataattat atttttttta aataattttt caaatttgtt caataataaa
    44881 tacataactt attttgtatt tttcttttca aagtttataa taaattcaac aggaaaatta
    44941 aatgaaaaca tataaagtag gaattattgg atttggattt ataggcaaag ttcatgctta
    45001 tggatatgaa aatttaaaat attattaccc gaattctcct tttagagttg aaattgaagg
    45061 tatttcaaca tcaagagaag aaacagcaaa aaatgttaaa gaaacctatg ggtttgaaac
    45121 gacttctgat tatagaaaac ttattgaaaa taaagaaatt gaaataattg atatttcaag
    45181 tcccaacata tatcattttg agcaattgat gtacgggata aaaaacaaaa aacatattta
    45241 ttgtgaaaaa ccacttgttt gcacaataga agaaacatta gaacttgaaa aaaaattaaa
    45301 aaattttgat aaaaaccatc aagtagtttt tcataataga tttttccctt caactataaa
    45361 aacaaaacaa tttatagaaa atgggtctat tgggaacccc acaattttta gaattgccta
    45421 ttaccattct ggaagtgttg ataaaaataa accaattggc tggaaacagg aaaaaggagc
    45481 aggtgtatta cttgatattg gctctcatgt tattgattta atttattatt ttttaggtaa
    45541 ttttaaaaat gtctctggca aaagtaaaat tctctatcct gaaagaaaga cgaaaaatgg
    45601 caaaattgta aaagttgagg ttgaagacca tattgttata aatgctgaaa tggaaaatgg
    45661 ggcaattggt attattgaag catcaaaaat agcaacagga acagaggatg agttaaaata
    45721 tgaaatatat ggaacagaag gagcaataag atataactca actcaaccaa attatctgaa
    45781 cttctataat agaaaagaaa cagaaagtgg ttttaagaaa ataccaactc ttggaaatta
    45841 tcctgaaagc aattttcctg gacctaaatt tactactggc tggataagag ggcatattca
    45901 ttctatatat tgtttcatta aatcaattta tgaaaatagc cctgcaattc cttcattttt
    45961 tgatggtatt tacaatatga aaataattga ggatataaaa agtggagatg aaaatgcctg
    46021 atgaaatagc aaaaaaaaag aagagaggta aaattttgca gagtaaaagt catgatgttt
    46081 ttttgaatta ccctgcaaga gattggggtg aagggatacc tattgggaat ggatttttag
    46141 gttcaataat ttatggagac ccaagtatgt taagtttcac tttaaataga agtgatgtct
    46201 gggattatag atataaggaa tttgacccag aaggcaaatt aaaatttaat cagttaaaaa
    46261 atcttttaaa aaagagaaat gagaaagcat ataaagattt tgtaaaagag acagaagacc
    46321 atagtagtaa atatccatat cctgcacctc aaacatgtgg aaggatgcaa ataagtttta
    46381 aatcagatgc tggaagatat tgtcaacgat taagtcctga aattgggaaa acatttattg
    46441 aattttctta taataagaaa aaaagagtta ttgaagtatt tattcctcct gaattaccaa
    46501 taatgtttgt taaaataaaa ggtaagaatt tagagccatt ttctattttt ttgaatcgtg
    46561 atggtttatc aataatacct gaacttatta aatcagaaat tccttctgaa aaaataaaaa
    46621 tagaatatgc aggaaataaa gaattattca aaatgattgg taaatttcct gatggtttat
    46681 attttgaggt tggtattaaa gaaaaagaag gtattttttc atcaatagat acaggaaaag
    46741 ggaaagtaga tattcaccct gatggtaaaa atgagattac tattgttctt ggagtagtat
    46801 ctggatataa aagagaaaaa cttgatgaag aaattgcaaa ggtaataaat aactcatcaa
    46861 agaactatac attattaaat accagacatt tacaggaatg gggaaaattt tggtctcagt
    46921 cctcaattac tattcctgat aaattgcttg aacatatatg gtatatcagt caatattttt
    46981 tgaattcaac attaaaatat gcaaatcaag cacctggatt atatggatta tggacatatc
    47041 atgatattac tccctggcgt ggggactatc atcttgatag aaatttacag gcggtattag
    47101 aaccactttt ttcatcaaat catatagaat tagcagatat ttattatgat ttttttattt
    47161 cagtactacc tgaaattcgt aagcaaacaa agaaatttta tagatgggat ggaatatctt
    47221 ttccaggaag tattggacca aaaggagaaa atatgggagg atattttcct gtatcacatt
    47281 gggaaggtac ctcagcttgg atatcattac ttttctggtg gaaatatcgg ttcactatgg
    47341 ataatgactt tttaaaagag aaagcatatc catttatgaa agaatgtgga atattttatg
    47401 aaaaatattt aggagaaaaa agagaagatg gtaaatatca tctatggcca tcgtatcttc
    47461 ctgaaacagg tgagaatagt atgaaagcgt ggggaacaga ttctgcaatt gatataggat
    47521 tattgagatt tttatttggt gctttaataa aggcagcaaa aattctggga aaggataaag
    47581 aagatataaa aaaatgggaa gaaatattat ccgggctacc tgattatcct acatgggatg
    47641 gagcaatagt agattatcag gaatggccag agttgtacga gaaagaaagc atatttactg
    47701 atgatttgag gattgcccct atttatccaa taggagatat tactcaggat agtcctgatg
    47761 aactattaga actgggtaaa agaacatttt atagtcttgg aaaacatcat gcgagaatct
    47821 ggttattaac tgaatctgtg gttgcatccc gtcttggcat atcagaattg tcttatgggt
    47881 atataaaaag atgggctcaa catattactc cacagggaat tggaacttat tctacaggaa
    47941 tatctattgg ctggcgtgat acatctctaa atgatggtat tcgtaaatta tataaattgg
    48001 tgccatggaa ctattcagta tttggtatag aatttccatc taattttatt tctgcattaa
    48061 acgaaatgct tttacaaagt tgtggtgaaa aaataagagt gtttcctgct attccaaagg
    48121 aatggaaaga agtgaaattt gagaatttta gagcagagag ggcattttta attagtgctt
    48181 atatgaagga tggagttgta aataagatag tagtaaaaag tattaaagga gaagaacttt
    48241 tacttgtgaa ccctgaaccc gcaaggaaat tttgtataaa gaaaaatggt aaaaaattag
    48301 gaataggaga gggaaaaatt ttcagattaa aaacaactcc ttcttcctgt tatgaattca
    48361 caataaaatg aatttatagc attttttgct attaaatttt taaaaatatc cagtgatatt
    48421 aagaatactc tcagggttta gattgtttca tagagataaa aaaaatcaat ggtttcgttt
    48481 ttccctgatg ctctcaacta taaagaataa tgcaagtaga acaattatag aggcaatatt
    48541 tccaagaaga agttttttat ttaaataaaa atcctttgcc tgaattatta aagcaacaat
    48601 tgttgttaag aacataaata ttgaagggta aaaaagaact tttgagttct ttcctctttc
    48661 agaaaggtat aaagcacata taagaagaac aactgctgca acgagttgat ttgaggcacc
    48721 aaatacaggc catattttct gccaactgcc aaaagcaaga tatccagcaa ttataattat
    48781 aaaaatagtg gcaaaatatc tatttttaaa aatatttatt ttaaatgtat caccaaacaa
    48841 ttcctcagaa atataccttg ttattcttgt agcagtatca agagttgtta aaacaaaact
    48901 atttattata acaattgcca gcatagttcc aaattttgaa ccaaaaattg gggatgttac
    48961 ctgtccatat ccaagtgcaa atgttcctat ccagttcccc cctttcataa gagagggata
    49021 attaagtaaa gaatttggtt cattccaata aagacccgca gtaacacata ttaaagcaag
    49081 aattgataaa accccttcta caagcatagc cccataacca atattttttg catatttttc
    49141 attattaatt tgttttgagg ttgttccact tgaaacaaga gaatgaaaac cacttatcgc
    49201 accacaggca ataataacaa acatcatggg ccaaagtgga ccatttgaag aagaatatga
    49261 aacaaagttt ggagtgtgaa tttgtggatg tgtaattacc agtcctaaat agccaaaaag
    49321 caaaccaaaa aataaaagga aagaagaaag ataatctctt ggttgaagta ataaattaac
    49381 aggtaataca cttgcaataa gagaataaag aagtagaatt acaatccacg cttttgttgc
    49441 ataggcagga cttataacaa caggatacct gcttcctaaa aagaaaagta agacataaag
    49501 aataagtgaa attaaagttg catgaatttg agggagatta aatttataaa cagcaacacc
    49561 aaaaaatatt gcaattatta atagagaaaa agttggtata acaatttttg gttcttcaat
    49621 aaatgttgta gcagtaacag cagcaaaaac agccacaaca agaataagag tgaaccataa
    49681 aaaaatagaa aagaaaattt tccctctttt gcttattaca gaagaagaaa tatctcctat
    49741 ggattttcct tcatttctta tactcaccat taaagaagaa aaatcatgta ctccacctaa
    49801 taaaattgag ccaaaaacaa tccataaaaa agttggacac catccccaac aaacagcagc
    49861 aattacaggt ccaagaatag ggccagcccc tgcaattgaa ccgaaatgat gtccaaaaag
    49921 aatagaccag tgttttacag gaacataatc aactccatct tcttttttat aggcaggtgt
    49981 tttttcatca ggatttactt cccataattt ttctaaatac ctgccataaa atacataagc
    50041 aatgagaaaa agaaaaatcc caattcctgc aactaataga gagttcattt cccccttctt
    50101 ctttttgctt gtttttttat atttttttaa ttatttttat tttaactgac agaagagtaa
    50161 aaatcaaatt atgttttatt aagtgctgtt tcaatgtgct tattgtctat ttttactttt
    50221 tctacaagtt tttcatcctt gaatatttct atcagatgac ttaccatttt actttatttg
    50281 gttaaaaaat aagtataaat cttgtgcata taaaaaaatt tcttgtttct atactaatct
    50341 atctttttaa ttagaatcta ttcagacctt tttagaagtg cttcccatct tttgtcaatt
    50401 tcttgttgta ttttgtcaag gagttcttta ttttctggtt ttagaaggtg agaaaatctt
    50461 ctttgcagtt taaaccactc aataactggt tttttctctt ttggcttata tgtcaatttg
    50521 tattttccct caacaacttc ataaagtggc cagatacatg tgtctgctgc aagacgagaa
    50581 atttctaatg taagttttgg gtcatgaggc cagccaagtc ggcagggaga aaggatatct
    50641 ataaaggcaa aaccatcaaa agttgtccct ttttcaactt tatttacaag gtcattccat
    50701 ttaccgggta ttgatgttgc aacataaggg atattatgag caaccattat ttcaggtaag
    50761 tttttttgcc attgtaattt accgggaata actttaccag aaggagaagt ggatgtatgt
    50821 gccccaaatg gagttgcaga agacctctgg at
//
LOCUS       DAOOVW010000007        49487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_444_length_49487_cov_8.871035, whole genome shotgun sequence.
ACCESSION   DAOOVW010000007 DAOOVW010000000
VERSION     DAOOVW010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 49487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49487
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_444_length_49487_cov_8.871035"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PLW95_01725"
     CDS             complement(<1..307)
                     /locus_tag="PLW95_01725"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOV21387.1"
                     /translation="MIKGTTKITGVFGNPVSHSLSPIFHNAAFQYLSLDFVYIPFEIK
                     PSFLKKGIEAIKFFNFKGVNLTIPHKKDAFFLVNEVDEEAKLLKVINTIKNEDGKLTG
                     "
     gene            378..449
                     /locus_tag="PLW95_01730"
     tRNA            378..449
                     /locus_tag="PLW95_01730"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:410..412,aa:Gly,seq:gcc)
     gene            765..1592
                     /locus_tag="PLW95_01735"
     CDS             765..1592
                     /locus_tag="PLW95_01735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HOV21388.1"
                     /translation="MGKNIVNYSSEKRFAKIQSLKDKEVFFIDLDGTVYIGNKIVDGA
                     VDFVNQLYENGKLYFFISNNSSKSKEDYVKKIRRYGINVKEKQIILSTDGLIDFLKSN
                     EIKNVFTIGTESFKKEIKDNEINPESKNPEFVVLGYDTELTYKKLVKGIYYVNNGVEY
                     IATHCDIVCPTENGPVPDVGTLIETFKLTTKKGPFKIFGKPNKEMIEFKIKELKVDIN
                     KVVLIGDRLYTDGVLAKNIGVTYILPLSGETNREMLESSNVLPDLIVEKLGDLVKYV"
     gene            1713..1784
                     /locus_tag="PLW95_01740"
     tRNA            1713..1784
                     /locus_tag="PLW95_01740"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1745..1747,aa:Thr,seq:ggt)
     gene            1808..3010
                     /gene="tuf"
                     /locus_tag="PLW95_01745"
     CDS             1808..3010
                     /gene="tuf"
                     /locus_tag="PLW95_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOV21389.1"
                     /translation="MAKEKFIRSKTHVNVGTIGHVDHGKTTLTSAITKVLAKENKAKE
                     MKYEEIDKAPEEKERGLTINISHIEYETETRHYAHIDCPGHADYIKNMITGAAQMDGA
                     ILVVSAPDGPMPQTREHILLARQVEVPAVVVFLNKMDSVEDEELVELVEVEVRELLSK
                     YGFPGEEVPVIKGSALKALECGCGKRECEWCGRIWDLLDAVDRYIPMPVRDIDKPFLL
                     AVEDVFSITGRGTVATGRIERGKVRVGDNVEIVGLKHEITKTVVTGVEMFRKELEEGV
                     AGDNVGLLLRGIDKDAIERGMVVAAPGSITPHTHFKAQVYVLKKEEGGRHTPFFHGYR
                     PQFYIRTTDVTGEIKLPEGVEMVMPGDNIEMDIKLIYPVALEKGQRFAIREGGKTVGA
                     GAVTEIIE"
     gene            3023..3175
                     /gene="rpmG"
                     /locus_tag="PLW95_01750"
     CDS             3023..3175
                     /gene="rpmG"
                     /locus_tag="PLW95_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOV21390.1"
                     /translation="MRNIITLVCSECGNKNYYTTKKKQTGQKKKIELKKFCPKCGKHV
                     LHKEGK"
     gene            3333..3406
                     /locus_tag="PLW95_01755"
     tRNA            3333..3406
                     /locus_tag="PLW95_01755"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3367..3369,aa:Trp,seq:cca)
     gene            3420..3605
                     /gene="secE"
                     /locus_tag="PLW95_01760"
     CDS             3420..3605
                     /gene="secE"
                     /locus_tag="PLW95_01760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOV21391.1"
                     /translation="MKKKISDFFNEVKAELKKVTWPEKKMLKRTTVIIVIFIFIMALY
                     IGIIDLIFSKVIILFLR"
     gene            3608..4135
                     /gene="nusG"
                     /locus_tag="PLW95_01765"
     CDS             3608..4135
                     /gene="nusG"
                     /locus_tag="PLW95_01765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOV21392.1"
                     /translation="MGKWYILHVRTGQEEKIRKLLENQLNKKEKQITQIVIPKEEIVE
                     RGKEEKKTTRRFWPGYMLIEIAPEFDNEILWHRIRTTTGVLNFLGAGDKPVPLQPEEA
                     KNVIGEVEKRKSKPTPKVEFKEGDKVEITEGPFMNFTAIVEEVYPDKGRLKVSVSAFG
                     RSTPLELSFWQVEKI"
     gene            4144..4569
                     /gene="rplK"
                     /locus_tag="PLW95_01770"
     CDS             4144..4569
                     /gene="rplK"
                     /locus_tag="PLW95_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOV21393.1"
                     /translation="MVEKKIKAVVKLQIPAGKATPAPPVGPALGQHGVNIMEFCKAFN
                     DVTKGKEGTVPVVLTIYEDKTFNFIIKTPPTSELIKKYANIVKGSAIPNREKIGKITR
                     KQIEEIAKIKLPDLNTKDIKEAMKIVEGSAKSMGIEIID"
     gene            4583..5284
                     /gene="rplA"
                     /locus_tag="PLW95_01775"
     CDS             4583..5284
                     /gene="rplA"
                     /locus_tag="PLW95_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012675317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOV21394.1"
                     /translation="MGKRYSTLLEKAEKDKLYTIEEAIDKLKEMHSVKFDETVELSIK
                     LGIDPKKLQQPIRGSVVFPYGIGKEVKILVFATGELAEKAKNAGADYVGGNELVEKIK
                     SGWLDFDAVVSTPDMMKVVSPLGKILGPRGLMPNPKTGTVTNNVEEIVKELKKGRIDY
                     KMDKEGNIQIPCGKISFDKEKLVENIIEAIKNVLSVKPSSVKGIYINNMYLSLTMSPS
                     LKLNSTRILQELKIS"
     gene            5457..5978
                     /gene="rplJ"
                     /locus_tag="PLW95_01780"
     CDS             5457..5978
                     /gene="rplJ"
                     /locus_tag="PLW95_01780"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOV21395.1"
                     /translation="MKKMNKNKKKEIVNWLTEEFKNSKIYLLVNFSGLSVLEMQNLRQ
                     LIKENNGDIKVIKSSLIERTFKNLSKDEAVNYLGGPIFVVWTKDGDEIEIIKKLYAFG
                     KRVGKIAVKGGVINNKISDAEFFEKLSKLPSIKEIQSELIYAIKFPVIRITNLMRSPV
                     VRIVNILNQISQK"
     gene            5997..6389
                     /gene="rplL"
                     /locus_tag="PLW95_01785"
     CDS             5997..6389
                     /gene="rplL"
                     /locus_tag="PLW95_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOV21396.1"
                     /translation="MAEEKNVLDEIVEKVEKLTAIELSELATKLEEKFGVKGMVGPVA
                     VSSVPGVAGQEEKKEEKTTFDVILKGFGSNKIQTIKEVRAITNLGLKEAKELVESAPK
                     PIKEKISKEEAEEIKKKLETVGAEVEIK"
     gene            6419..13978
                     /gene="rpoC"
                     /locus_tag="PLW95_01790"
     CDS             6419..13978
                     /gene="rpoC"
                     /locus_tag="PLW95_01790"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOV21397.1"
                     /translation="MERKGSSFSFLEEKNEFNNKVVENVEIPDLLLVQKKSYEEFLQQ
                     NILPGKREVKGIEEVLRKIFPIESKNGNLILEYVNYTIELPVYSVEECIENGLTYDGK
                     FKIKVRLAIKNHETGKIISVKEQEVYIGSIPLMTESGSFIINGIERVVVNQLLRCPGV
                     YFKEEEINGQKTLYSAKVYPLRGLWIEFHIDSHNCLILFLGRKKVIGTMFLKALGLTN
                     EDIVKEFYGSEEKMPENSILRQTLLRDTTKTREDALKEIYKELRPGYPPIIKEAESFF
                     NSLFFSDELYSLSEAGRAQINRKLNLNLTQQHLTKEDIIGIIKYLLNLTEKGEGTTDD
                     IDHLGNKRVRTTGEIVKEHLYEGFLRMRRYCEEKMKIIPPEEVDKKVTPQDFINGKVL
                     MTVLNDFFARNQLSQFLEKINPLSEITHKRRVTAVGEGGIKERKRAGFEVRDVHYTHF
                     GKICPIETPEGANIGLIGSLTVHSRIGELGFIVTPYFKIENGKVTDKIEYLNANDEEN
                     FKIAPPDVEVDPKTKEIKEEVVKVRVKDGLFEVVPKNEVDYIGVSPIQIVGVTASLIP
                     FLEHDDSNRALMGSNMQRQGVPLIKSEVPLVKTGMERFVIRDSGSVVKVRSDGIVKYV
                     SAEKIVIEKQEKGIFPWEKEDIYYLKKFTRTNQDTCFHQRPVVDIGDRVKKGQIISDG
                     LATSKEGELALGKNLLVAFMPWHGYNYEDAIILSERMVKEDVFTSIHIREFPVEARET
                     ELGPEEITADLPNVPESRLKNLDKNGIIRVGAEVEADDILVGKITPRGESELTPEEKL
                     LRVIFGEKAKDVQNTSLKVPPGIKGVVVNVRKYERRENIKEEDIKKELNKVNKEYKEK
                     EKIIKEIIKEQIEKTLKLHNIKAKVTTTKETWNKIVEKIQDEKVKEKLQKLIDIENEE
                     IKKLKKELRDKENKIKKGPELEMDVIYKIVVEIAVKKKVSVGDKMSGRHGNKGVISKI
                     LPEEDMPFIEDGTPVDIILNPLGVPSRMNIGQILETHLGIALKTLGYSYETPIFKSIK
                     ELEIKELLKKGGLSENGKMTLYDGQTGEKFSQPITVGYIYMMKLIHLADEKIHARATG
                     SYSLITQQPLGGRSQFGGQRLGEMEVWALEGYGAAHTLKEMLTVKSDDTEGRKQIYES
                     IAKGIDFKSNNTPESFNVLKRELQGLGFDLRVEKRNIGGKSKEIVTIKVASPTVMRSW
                     SHGEVKKAETLNYRTLRPEREGLFCERIFGPTKDYECACGKYKKLRYKGIICDRCGVE
                     VTTKRVRRERMGHIELATPVSYVWLFKSSSNWMGMLLDLSQSELEMVFYYARYIVIDP
                     GKTNLTKKQLLTESEYYEMTEKHGNAFRAEIGAEAIFHLLKEIDLISLEEQIKQSLSK
                     MKKNERETKRKLIKKLRMIQGLLKTNTKPEYLVTNIIPVIPPDLRPLLPLDGGKFVAS
                     DLNDLYQRVINRNNRLKKLIKLQAPDIIIRNEKRMLQESVDALMDNGKHGNTILGKGR
                     RPLKSLADALRGKQGRFRQNLLGKRVDYSGRAVIAVGSELKLNECGVPKEMALELFSP
                     FVLRELRKKEYFHTLGSAKRAIRENKEEVWEILERVSKHHPVLLNRQPTLHRLSIQAF
                     EPRLIEGNVLKLHPLVCSAFNADFDGDTMSIHIPLLPETILEAELLMKATTHILSPAS
                     GKPIVSPSRDIGMGCFYLTFISDEEEYPEDERKYISSFEEAKFLYHNGFVELHRPIRV
                     RDEIGEIITTTVGRILFNEILPFELPFKNEEFEVKKLESLIKEIFNKFGYEKTVEVLD
                     GLKEIGFLYSTIGGITIGIDDLKIPEKKKDIIERATKEAEKVERNYKKGIITEDERYN
                     RIVDIWTSTTNEVCDYLMDTIEKDRSIKPFRINPIYLMVTSGARGNKNQVNQLMGMRG
                     LMIRPTKKVTGGIGEIVETPVISNFREGLDLLEYFTSIHGGRKGLVDTALKTSDAGYL
                     SRRLVDVAHSVIVTEEDCGTLEGILIGPLVEEDEVIIPLEERIIGRVAVDNVVDIVTD
                     EEIVRAGEVIDEEKAKRLVEAGIQRIRIRSVLTCKAEKGVCAKCYGWDLSRGIVVNIG
                     EAVGVVAAQSIGEPGTQLTLRTFHTGGTASRGIGSSQFTVKKDGILKYEDNLKTVMNK
                     EGKMVVVHREGNVKIVDERGREFEKYTLRVGSIIELKDGSKVKEGQLIASWNPYSTPI
                     ITDKEGFIKYEDIEIGKTANKEMIIIPHKEDKDPRIILRDKKNNIIDGYHIPEGARIV
                     VKEGEKVEPGDIIAEKVHTTARAQDITAGLPRVSELFEARVPRNPAVLSEIDGEVEIM
                     PSEKGTRKIKVKNEETQTEKIYIIPYGKHLLVGNGDYVIAGRKLTDGPVVLQDILKIQ
                     GEKKVQEYLLNEIQKVYRVEGVTISDKHIEIIVRQMLSKVKVEDAGDTYLLEGEEISR
                     VEIQKINESLPKGKNPATYKSLVLGITRVALSSSSFISASSFQETMKVLTNSAILGAE
                     DYLEGLKENVILGRLIPAGTGKFTKEITEEKAEFIETQK"
     gene            13993..14388
                     /gene="rpsL"
                     /locus_tag="PLW95_01795"
     CDS             13993..14388
                     /gene="rpsL"
                     /locus_tag="PLW95_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOV21398.1"
                     /translation="MPTVNQLIRKKRRLRRKKSKSTALQSNPFKKGVCLYVKTMTPKK
                     PNSALRKIAKVRLSNGKEVIAYVPGIGHNLQEHSIVLVRGGRVRDLPGVKYHIVRGTF
                     DAGGVEGRKKARSKYGAKKPKEEEKKQEG"
     gene            14389..14859
                     /gene="rpsG"
                     /locus_tag="PLW95_01800"
     CDS             14389..14859
                     /gene="rpsG"
                     /locus_tag="PLW95_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOV21399.1"
                     /translation="MPRRKRVIKKRIDEDPRYNDYEIEKFINKLMWDGKKDLAYRIFY
                     DMMDQIKEKMNQDPLPVFKKALNNVKPKLEVRPRRIGGATYQIPIEVSPHRGLSLAIR
                     WIVETARSKKGKSIVDRLVEEILEAYKGEGASIKKRDETHKMAEANRAFAHYRW"
     gene            14896..16935
                     /gene="fusA"
                     /locus_tag="PLW95_01805"
     CDS             14896..16935
                     /gene="fusA"
                     /locus_tag="PLW95_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOV21400.1"
                     /translation="MDKIRNIGIIAHIDAGKTTTTERFLYYTGKTHKIGEVDEGTAVM
                     DWMEQEKERGITISAAATTCLWKGYKINIIDTPGHVDFTAEVERSLRVLDGAIGVFCG
                     VAGVQSQSETVWRQSEKYNVPKLVFVNKLDRVGADFFRVIDEIKIKLNTSPLIMNIPI
                     YDDNEEFIGLIDIIEEKEVIYNETYETEAEVKDIEEKNREKFLYYKKNLIEKVAEVDD
                     YLMGKYVEEEEIKKSEIKRAIRKGTIKNLFCPVFCGSALKNKGTLFLLDGIVNYLPSP
                     VDRGKIEGFSTDKKKKIERHPNENQPFSGLIFKVYNDIHLGRVLYTRIYSGKIKKGEK
                     VYNSSRNVKEKLMRILEVHTNKYYEKEQGKAGEIVALLGPKKTYTGDTICDELSPIIF
                     ESVEFPEPVIYLAVEPKTMSQHEDVYNALLKIADEDPTIKIRTDAETGQMIIMGMGEL
                     HLDVVIGRLKREDKLEVKVGRPHVAYRETITVPSIGEGKFIKQSGTKGIYGHVVLKLE
                     PLERGKRFIFESKVDSEKIPDEFIPAIEEGIKEGMEVGTLGGFPVIDIKAVVIDGSFH
                     PTDSDEVAYKIASTIAFKDAYRKGYPILLEPIMKVEITVPEEFLGEILDDLNFRKGKI
                     ESIETLKDLKIIVARIPLRKIFGYTTTLRSLTQGKGSCIIEPLCYESVNEEILTK"
     gene            16993..18195
                     /gene="tuf"
                     /locus_tag="PLW95_01810"
     CDS             16993..18195
                     /gene="tuf"
                     /locus_tag="PLW95_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOV21401.1"
                     /translation="MAKEKFIRSKTHVNVGTIGHVDHGKTTLTSAITKVLAKENKAKE
                     MKYEEIDKAPEEKERGLTINISHIEYETETRHYAHIDCPGHADYIKNMITGAAQMDGA
                     ILVVSAPDGPMPQTREHILLARQVEVPAVVVFLNKMDSVEDEELVELVEVEVRELLSK
                     YGFPGEEVPVIKGSALKALECGCGKRECEWCGRIWDLLDAVDRYIPMPVRDIDKPFLL
                     AVEDVFSITGRGTVATGRIERGKVRVGDNVEIVGLKHEITKTVVTGVEMFRKELEEGV
                     AGDNVGLLLRGIDKDAIERGMVVAAPGSITPHTHFKAQVYVLKKEEGGRHTPFFHGYR
                     PQFYIRTTDVTGEIKLPEGVEMVMPGDNIEMDIKLIYPVALEKGQRFAIREGGKTVGA
                     GAVTEIIE"
     gene            18256..18567
                     /gene="rpsJ"
                     /locus_tag="PLW95_01815"
     CDS             18256..18567
                     /gene="rpsJ"
                     /locus_tag="PLW95_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003672692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOV21402.1"
                     /translation="MEEKRIKITLKGFDHRLLDKSIKEIIELAKKTGVRISGPVPLPT
                     KREVYTVLRSPHVNKKSREQFEMKIHKRLIEIIEPTGRTIDALTKLSLPEGIDIKMDL
                     S"
     gene            18579..19226
                     /gene="rplC"
                     /locus_tag="PLW95_01820"
     CDS             18579..19226
                     /gene="rplC"
                     /locus_tag="PLW95_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOV21403.1"
                     /translation="MRIGLIGKKLGMSRIFNENGDVIPVTIIEVKDNYVIGIKKESEK
                     DNYSAIQIGCQEVEDKKLRKSELGKLKKNNLPSLKVIKELRMKNDDEVNDFKIGQKLN
                     VEIFKKGDLVDITGKTIGKGFQGVVKRHGFKGGPATHGSMSHRKPGSIGGTTPARVLK
                     GQKMAGHMGNEKQTIQNLEVVQIMHDKNLILIKGAVPGPKNRYLLVRKAIKGLSK"
     gene            19223..19870
                     /gene="rplD"
                     /locus_tag="PLW95_01825"
     CDS             19223..19870
                     /gene="rplD"
                     /locus_tag="PLW95_01825"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOV21404.1"
                     /translation="MRKIPIYDINGEIVGEEEIEIENEKINKDVLYYYVNAYLTNQRK
                     GTSSTKTRGDVSGGGKKPWRQKGTGRARVGSIRNPIWKGGGVVFGPKTKEYKVRLPKK
                     IKRMALIEAIKDKIIEDRFLLIEMSNLNEIKTKNVYNFLKKIGSNKEKILFILNKGDV
                     NKEVIIKSIRNIDTIEYDYSDKINAYEILKVDKVFLDDKNIFGVIKKTLEKENGS"
     gene            19860..20144
                     /gene="rplW"
                     /locus_tag="PLW95_01830"
     CDS             19860..20144
                     /gene="rplW"
                     /locus_tag="PLW95_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006694704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOV21405.1"
                     /translation="MAVEAYKIIKIPLISERATDLQAENKYVFIVDKKANKNQIKEAI
                     ETIYKVHVKKINVINVPKKKKKYRMNIEGFRSGYKKAIIKLKEGEKIAIT"
     gene            20131..20961
                     /gene="rplB"
                     /locus_tag="PLW95_01835"
     CDS             20131..20961
                     /gene="rplB"
                     /locus_tag="PLW95_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012459022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOV21406.1"
                     /translation="MPLRKLKPVTPGQRHAILDDFSDITKKYPEKSLVIGKRKKGGRN
                     NTGRITVRHRGGGHKKLYRIIDFKGRKNMEGKVVSIEYDPNRSSRIALVHYTDGIKSY
                     LIAPYGLKVGDIINCGENAEIKTGNRLPLRNIPEGLQIFNIEMVPEKGGQIVRSAGTL
                     ATLMVKGEKYAQVRLPSGEVRLFDLNCFATLGQVSNIDHKYVSLGKAGRTRHLGIRPT
                     VRGVAMNPVDHPHGGGEGRSKGHQSQSPWGWSTKGHKTRKKKKPSDKLIVERRKRSKG
                     "
     gene            20958..21239
                     /gene="rpsS"
                     /locus_tag="PLW95_01840"
     CDS             20958..21239
                     /gene="rpsS"
                     /locus_tag="PLW95_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOV21407.1"
                     /translation="MRSKKKGPYVDLKLMKKVEKLRREGRKEIIKTWARASFVIPEFV
                     GYTFAIHNGKNFLNVYITENMVGHRLGEFAPTRVFRGHGAHTERTREKT"
     gene            21252..21593
                     /gene="rplV"
                     /locus_tag="PLW95_01845"
     CDS             21252..21593
                     /gene="rplV"
                     /locus_tag="PLW95_01845"
                     /inference="COORDINATES: protein motif:HMM:NF012462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOV21408.1"
                     /translation="MEVKAISKYIRVSPRKLKMIKDIVIGKEINEAIQILESLPKRGS
                     EYLIKTLKSAQSNAEGKKKGGIWMVKNLLVDSGPSFKRFRATTMGRGVMVRKRTSHIT
                     VILQDIEEGEK"
     gene            21593..22270
                     /gene="rpsC"
                     /locus_tag="PLW95_01850"
     CDS             21593..22270
                     /gene="rpsC"
                     /locus_tag="PLW95_01850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOV21409.1"
                     /translation="MGQKVNPIGLRLGITEDWRTKWFASKKDFKDSLKEDIFIRDNIN
                     KKYPRGTISKIEIEKVIKEKIKIIIYTTRPGVILGRKGSEINQLRDELIGKLKKQVSI
                     DVVEVNPPGKSAQFLADTIVIQLEKRFSHRYAIKRAMALAMESGAEGIKVRISGRIGG
                     VEISRSEQYIEGKVPLHTLRAKIDYATATAFTRSGTIGIKVWLYLGEVLQGKRQEDKN
                     AVNAKES"
     gene            22245..22667
                     /gene="rplP"
                     /locus_tag="PLW95_01855"
     CDS             22245..22667
                     /gene="rplP"
                     /locus_tag="PLW95_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOV21410.1"
                     /translation="MPLMPKRVKYRKAQRGRRKGKAMRGSTLYYGDYGLKIMEPCWLT
                     NVQIEAARVAITHHLKTKGKVWIRIFPDKPVTKKPAETRMGKGKGDVSHWVAVVLPGR
                     IAFEIVGVDEETAVGALKRASSKFPVKSKIVKRQKIVS"
     gene            22677..22889
                     /gene="rpmC"
                     /locus_tag="PLW95_01860"
     CDS             22677..22889
                     /gene="rpmC"
                     /locus_tag="PLW95_01860"
                     /inference="COORDINATES: protein motif:HMM:NF013027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOV21411.1"
                     /translation="MKKDEIKKWREATEEEIGLKIVELEKQLFNLLPQVKMGQLKNCS
                     VLKNIRKNIAILNTIKREKSIQRVKK"
     gene            22886..23149
                     /gene="rpsQ"
                     /locus_tag="PLW95_01865"
     CDS             22886..23149
                     /gene="rpsQ"
                     /locus_tag="PLW95_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOV21412.1"
                     /translation="MNNSREIEKVGVVLSDKMDKTRVVSVEWKSAHPLYNKILRKRKK
                     FYAHDEDNITKVGDTVRIVQTRPLSKLKRWRIVEIIEKTERQK"
     gene            23146..23514
                     /gene="rplN"
                     /locus_tag="PLW95_01870"
     CDS             23146..23514
                     /gene="rplN"
                     /locus_tag="PLW95_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOV21413.1"
                     /translation="MIQLRTTLIVADNTGAKKIMVIGFIGVSKKRYARTGDIVICSVK
                     DALPDGIVKKKEKVKAVIVRTVKPIKRKDGTILKFDHNCAIIVDNEKNPRGTRIFGPV
                     PRELRDKNFTKIISLAPEVV"
     gene            23514..23837
                     /gene="rplX"
                     /locus_tag="PLW95_01875"
     CDS             23514..23837
                     /gene="rplX"
                     /locus_tag="PLW95_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOV21414.1"
                     /translation="MGIKIRKNDFVCVIKGNDRGKKGRVIKVFPEEERVLVEGVNFVK
                     KHTRPRSVDMQGGIIQIEKPISISNIRYFCLKCGKAVGLGIKKLSDGTKVRYCKKCNE
                     IVEEK"
     gene            23837..24382
                     /gene="rplE"
                     /locus_tag="PLW95_01880"
     CDS             23837..24382
                     /gene="rplE"
                     /locus_tag="PLW95_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOV21415.1"
                     /translation="MEPRLKEKYKEVVINHMMEKFNYKNKMQVPRLEKIVINIGIGKD
                     NKDTKAIESAEKELSLITGQKPVITKAKTSIAAFNLRKGDICGLMVTLRGKRMWEFLD
                     RTIVAALPRVRDFNGLPVNSTDGRGSYTIGIKEHVIFPEIDYNTIYKIRGMNITLVTN
                     AKTPEETLELLREIGLPIRRE"
     gene            24384..24569
                     /locus_tag="PLW95_01885"
     CDS             24384..24569
                     /locus_tag="PLW95_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008633399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOV21416.1"
                     /translation="MARKALIEKSNREPKFKVRGKNRCFRCGRARGYYRKFGICRLCF
                     RELASKGEIPGVKKASW"
     gene            24607..25008
                     /gene="rpsH"
                     /locus_tag="PLW95_01890"
     CDS             24607..25008
                     /gene="rpsH"
                     /locus_tag="PLW95_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOV21417.1"
                     /translation="MTVTDPIADMITRIRNGNLNYKRSVDIPFSKMKEKIAIVLKEEG
                     FIKNYEIIEIENKKVIRIYLMYYPDRKRVITEIKRVSKPGARVYIKKENTNKVIKNGF
                     GMAVLTTSKGIISDKKAREIGIGGEILFTIW"
     gene            25018..25587
                     /gene="rplF"
                     /locus_tag="PLW95_01895"
     CDS             25018..25587
                     /gene="rplF"
                     /locus_tag="PLW95_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006342209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOV21418.1"
                     /translation="MARLGKRPVEIPEGVKGKVENNRIIIEGPKGKLEKEVLSYLKVE
                     IIDNNKIFVKNIIPEENRKLYKKGGEFQGLMRSLIKNMVVGVKDGYEKVLEIHGVGYK
                     GEVKEKKLVLTLGFSHPIEIDIPFDVSVEVARNTVIFVRGIDKQRVSSFAAIVKKIAP
                     PEPYKGKGIRYRGEYVRHKAGKAAVGTGQ"
     gene            25584..25964
                     /gene="rplR"
                     /locus_tag="PLW95_01900"
     CDS             25584..25964
                     /gene="rplR"
                     /locus_tag="PLW95_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOV21419.1"
                     /translation="MKIKKEEKRKRKHLKVRRKVKGNWERLRLVVFRSQKHIYASFVN
                     DDETPCKVLATVSTLSEGFKKYSEGKNIKTWTKDAAGIVGQLVAEKAKEIGIEKITFD
                     RGGYKYTGRLKVLAESARKGGLKF"
     gene            25958..26443
                     /gene="rpsE"
                     /locus_tag="PLW95_01905"
     CDS             25958..26443
                     /gene="rpsE"
                     /locus_tag="PLW95_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOV21420.1"
                     /translation="MLKENEITQQENEKQAVIEIRRVTKVTKGGKNLNFRAVVVVGDG
                     KGKCGYGIGKAVEVPDAIKKGVDKARKNMIEIPIVNTTIPHDITAKSSSSIVILKPAV
                     KGHGMVAGRTMRAIFEVIGIKDITSKCLGSTNALNVLSATIKALSQLKKKKEDKKNET
                     E"
     gene            26430..26885
                     /gene="rplO"
                     /locus_tag="PLW95_01910"
     CDS             26430..26885
                     /gene="rplO"
                     /locus_tag="PLW95_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOV21421.1"
                     /translation="MKLNELKVPYGAKKKRKRVGRGDASGHGGTSTKGHKGHKARSGY
                     TRSFNFEGGQMPLVRRLPKRGFTHLKEKIVEIVKIEELENKFNDGENVDVELLKKEKL
                     VKKGEFVKILGNGKLTKKINVSANAFSKKAKEKIENVGGKTILLEKIKK"
     gene            26882..28195
                     /gene="secY"
                     /locus_tag="PLW95_01915"
     CDS             26882..28195
                     /gene="secY"
                     /locus_tag="PLW95_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOV21422.1"
                     /translation="MIDAFKDTFKIADLRRKIGITIFLISVYRLGCYIPSPGINGQAL
                     ASFFENFPNTLFQLVDLFAGGALRNATIFALGIMPYISISIILELLSSIIPYFENLVR
                     GGVEGRRKLTQLTRIGTVILCLFQGFAISLWLENPEHFAGYVIVPNPGWTFKIVTMLT
                     LTAGTIFLMYLGEQITEKGVGNGISLIITASILSEMPIAVHRIAQLVSAGEISLFVVL
                     LLIVLIFIVVAAAIIVNESERRIPIQYAKRVVGRKVYGGQSTYLPIKLNQGGVIPLIF
                     AISILTFPATILTFSENKVIQKVAAYLSPSSGPGMFLYIVLIIFFCYFYASIIFSPDR
                     VAEDLQKYGGYIQGIRPGKSTALYLERILNRITLPGALMLAAIAIFPYIIMSAYKKIP
                     YIVAHIFGGIGLLIVVSVLLETIRQIESHLLLRHYQGFIKKEGIR"
     gene            28192..28833
                     /locus_tag="PLW95_01920"
     CDS             28192..28833
                     /locus_tag="PLW95_01920"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOV21423.1"
                     /translation="MKKILILLGPPGAGKGTVGRNISSFYNLPLISSGDLLRDNVKNH
                     TQLGEKAYFYIKKGELVPDEIVEDIIKERIGHEDCKNGFILDGFPRTLKQAEDLNKIL
                     MNNNKVIVIYLSASDHFLINRLSNRRICEKCGYIYHLINLPPKKDGICDKCGGRLIQR
                     EDDKSEVVKNRLDVYHSYTGPIIDYYKKNGNFYEISGEGELEDTLSEIKRILK"
     gene            28830..29570
                     /gene="map"
                     /locus_tag="PLW95_01925"
     CDS             28830..29570
                     /gene="map"
                     /locus_tag="PLW95_01925"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010073719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOV21424.1"
                     /translation="MIVQTSDEIEKVKKACVIVREILEGISEIIKPGISTYDIDELAY
                     EMMKKREVVPAFKDYGGFPANICVSINEEVVHGIPKKERILKEGDIVSVDIGVIYDGF
                     YGDCADTFPVGEIKQIHKKLIDVTKKAFYIGVDYIKDGVMTGDIGNSIQQFVESNGFS
                     VVKAFAGHGIGKKLHDFPELANFGNKGEGEILKDGMVICIEPMVNEGTWKVKILDDGW
                     TAITSDRKYSAHYENEVLVRKEKGEILS"
     gene            29571..29786
                     /gene="infA"
                     /locus_tag="PLW95_01930"
     CDS             29571..29786
                     /gene="infA"
                     /locus_tag="PLW95_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOV21425.1"
                     /translation="MNKEEKIRLKGEIINALPGTTFEVKLENGHKVFAHLAGKMRMHY
                     IKIIPGDEVLVEISPYDLTKGRIIKRY"
     gene            29800..29925
                     /gene="rpmJ"
                     /locus_tag="PLW95_01935"
     CDS             29800..29925
                     /gene="rpmJ"
                     /locus_tag="PLW95_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015851088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOV21426.1"
                     /translation="MKVRASVKKICPKCKIIRRKGIVRVICTNPQDGPKHKQKQG"
     gene            29934..30347
                     /gene="rpsM"
                     /locus_tag="PLW95_01940"
     CDS             29934..30347
                     /gene="rpsM"
                     /locus_tag="PLW95_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOV21427.1"
                     /translation="MARILGVEIPDNKKIEISLTYIYGIGKPTAKKILSDTGVSPEKR
                     VKELTDEELSKITNYIQRNFKVEGDLKKEISENIRRLVEINSYRGFRHKRSLPVRGQR
                     TRTNARTRKGPRKTVGIVRDKTARKAMRQEKGKEE"
     gene            30349..30741
                     /gene="rpsK"
                     /locus_tag="PLW95_01945"
     CDS             30349..30741
                     /gene="rpsK"
                     /locus_tag="PLW95_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOV21428.1"
                     /translation="MAARKYVKKRKKRVSIERGIVHIKATFNNTIVTLTDLEGKVLLW
                     ASAGSIGFKGTRKGTPFAAQLVSENVAKRAINMFNMKEVSVFVKGPGPGRETAIRALQ
                     STGLNVVSIKDVTPIPHDGCRPPKRRRV"
     gene            30741..31361
                     /gene="rpsD"
                     /locus_tag="PLW95_01950"
     CDS             30741..31361
                     /gene="rpsD"
                     /locus_tag="PLW95_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOV21429.1"
                     /translation="MAKVVETDCKVCRKYGIKLYLKGIRCESDKCAIDPRRKNIRLIK
                     SKKTSEYGHQLREKNKVKIYYGVRERQFRRYFEMAKKQKGVTGENLLQLLERRLDNVV
                     YRANFVSSRKQAKQIISHGAVIINGRKADRDGYIVKEGEEIKIKTNIKYIEKVKENID
                     IFKHRQIPTWMKVNNENYSIEIVRYPTREEVSIPVDEQLIINLYSK"
     gene            31380..32360
                     /locus_tag="PLW95_01955"
     CDS             31380..32360
                     /locus_tag="PLW95_01955"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOV21430.1"
                     /translation="MKEFLFPSSFIWDEDTYRENYGIIEIQPLERGYGVTLGNLYRRI
                     LLSSIPGISITSLKIDGVMHEFSTIEGVKEDVVEIVMNLKNVALKPVISEFPHKVEIE
                     IEGKDEIVAGDLIPDGSAYVINSGLHIATVDPTKKYKMEIEITNGFGYLPVEKMKLMI
                     STPPIGTILIDGIYSPIRKVSFHIENIMVGQSVDYEKLVLEIGTTGAITPKDAVSYAT
                     DLILSHLNLIKDKQTIGRKEVKKEEVGKIDLDIPISDLKLSTRVINALTGKNIKTIRD
                     LINTPREKLEELKNLGKKSIKEIENILKKRDIKLLTLKELAEKQEEEDET"
     gene            32350..32697
                     /gene="rplQ"
                     /locus_tag="PLW95_01960"
     CDS             32350..32697
                     /gene="rplQ"
                     /locus_tag="PLW95_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012858383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOV21431.1"
                     /translation="MRHKKDIKKLSRNKSHRKGLIRNMTISFFQSNEIRTTEVKGKQL
                     KRVVERMITLGKKQDLKSTRMLNAFLNHPESVKKVKEVAKRYSDRPGGYTKIIKLSTR
                     RGDGAREVIIKLV"
     gene            32701..32892
                     /locus_tag="PLW95_01965"
     CDS             32701..32892
                     /locus_tag="PLW95_01965"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOV21432.1"
                     /translation="MNKNLKLLDKVYKKENIYRLIVILAKRAHEIISGAPAKIKSDES
                     GNAPHELVIEEHLLSGDKK"
     gene            32972..34072
                     /locus_tag="PLW95_01970"
     CDS             32972..34072
                     /locus_tag="PLW95_01970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOV21433.1"
                     /translation="MNDFTQLLKCMKEKDASDLHIKFGRKPIFRIKGELEEVEEWENI
                     FNDEELNKICSIIIPSEQIEIFKKQKEMDIAFEQEEVGRYRVNAFWQRGHIGLVLRRI
                     KDVIPTIEELNLPPVLNKIASFSDGLVLVTGPTGCGKSTTLASMIENINKNKVVHIVT
                     IEDPIEYIYKDKKSIIDQREVGIDTIAFDSALKRVLREDPDIILIGELRDVDTFQAAI
                     NACETGHLVFATLHTIDTITTITRILDFFPSSQHEQVRKIVAYHLRATICQKLLTKKD
                     NSGLVPVCEIMIVNSVISKLIQDNKLNKIYAAMASDKEVGMQTFNQHLVQLIQNDIIS
                     KEEGFAASPSPHALEMNLRGIYLDEDSKIIGE"
     gene            34044..34757
                     /locus_tag="PLW95_01975"
     CDS             34044..34757
                     /locus_tag="PLW95_01975"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOV21434.1"
                     /translation="MRTQKLLENRKEPDLFFSISIISIILILSLIFSFLFRNLTIEEK
                     LFKGGIFTFLTEVGIIYFSFIKYPEYFKNIFSNFSLFFSGFKYYLIIIPIISIVSLIV
                     YAIFEQLHINVEIQKMVSFYLEIKSIKIILFMFFLSCFIAPLTEEIIFRGILYKSLRN
                     NFSFSIAVILSSLVFSLFHNDLFTFAGIFIFGCFLSIIFERHRYLWPSIGLHFFNNFF
                     TNLFVFFIKFHQAGQNFVI"
     gene            34778..36028
                     /gene="purD"
                     /locus_tag="PLW95_01980"
     CDS             34778..36028
                     /gene="purD"
                     /locus_tag="PLW95_01980"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOV21435.1"
                     /translation="MKILVIGSGGREHCICWKIKQNKNVRLFALPGNGGISEIAECIK
                     GDIEDFSDAFNIIKNKGIDFVVVGPENPLVYGIVDFLKEKNIKAFGPIKKGAILEGSK
                     CYAKEFMGKYNIPTANFQIAESYESAIKIIEKKEVPYVIKYDGLAAGKGVRVIESFEE
                     GEKYLKEIFIDNIFKSNEKKIIIEDCLIGKELSYLIFTDTISYIPMVPAKDYKRVFDG
                     DKGQNTGGMGSYSPPLFFNEELDNKIKNEIVERTIYGLRKENIDYRGVLYFGLMITEK
                     GPYVLEYNVRFGDPETQVILPRMETDIIDVMTAVCEGELKKIDIKWSNKKSVCLILSS
                     GGYPGGYETGKEIKNLEKVEKNVLIFHSGTKKENGKFYTNGGRVLGITALNSTIEKAR
                     ENVYKSASTIDFEGKHYRKDIALF"
     gene            36044..37249
                     /locus_tag="PLW95_01985"
     CDS             36044..37249
                     /locus_tag="PLW95_01985"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF019736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HOV21436.1"
                     /translation="MRKKVIIFGAGQIGRGFIGEICYSSGYFLVFVDVFKNVIDLLNK
                     EKKYPLWILGKEKEEKFIENFKGIYFDDKESVSNEIKEANLIFTAVGVNNLKSLAPLI
                     SEGILKKFSANKSDYLNIIICENLLGSGKILYSEVLEYLPPEFKEVVFQKVGFVESVV
                     SRMVAPITDEIKKINPLIVRVEPYNKLPVDKKAFKGEIPDIKGLYPVENLYPYEELKI
                     FVHNLSHACLAYLGYLSGYTYIWQCMEDKKILKIFKGVLNEVKEALIKKHKFDEKEID
                     DYIYDLQTRFKNKLLGDTVFRVGRDPLRKLGPDDRIIGSINLCLSQNIFPANICVVAS
                     ACLCYNYQNDKQAVELEEIIRNNSVEFVLKEICKLENKEIIDEILKHYEEIKNESSTS
                     KRNREFRNY"
     gene            37203..38243
                     /locus_tag="PLW95_01990"
     CDS             37203..38243
                     /locus_tag="PLW95_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent dehydrogenase"
                     /protein_id="HOV21437.1"
                     /translation="MKAALLKEIENLEIIDIEKPIPGENEIVIKVKVCAICGTDIKVY
                     HHGHKLIVFPRITGHEVSGIVAEKGKKVSEFKESDKVAVAPAIPCGRCWYCRKGQQSM
                     CDNLETIGYLYNGGFAEYMRVPENAVLNGCVNKLPENLSFEEAAIAEPLACVINGQIL
                     SMLEIGDTVVIIGAGPIGCFHSQLAKINGASKVILGDISSERLEMAKFTGAKLVDMSK
                     ENIKEVVKEETEGKMADRVIVAAGAKEAQETAVEIVAKRGSVNFFGGLPKEVPFINLN
                     SNLVHYGEFFITGTHGSSPVHNKIAISLLANKRIDGTKYITHKFPLEEIKQALKTAED
                     KKGLKVIVIPEK"
     gene            38704..39666
                     /locus_tag="PLW95_01995"
     CDS             38704..39666
                     /locus_tag="PLW95_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOV21438.1"
                     /translation="MEKIDRKLAIKLLKQMMEIRKFEDKIMELLSKNIAEGGSHLYAG
                     MEGTGVGAISVLRPDDYITSTHRGHGHAIAKDGDLKALMAEILGKKTGVCKGKGGSLH
                     LADLSKGNLGANGIVGGGLGIATGAGLSIKLQKQDKVVICFFGDGATNNGIFFECLNM
                     ASLWKLPVIYLCENNKYGMSVSVERASSVVDLTKKALAFDMPSENVDGQDVLAVREIV
                     NKWVNYARDGNGPSFIVSNTYRYYGHSRSDPRVYRTKEEEKFWKERDPILIFSDKMKE
                     VGVLTEEEIREIDELTDKEIEEATEYAINSPDPQPEELYTDLYV"
     gene            39680..40654
                     /locus_tag="PLW95_02000"
     CDS             39680..40654
                     /locus_tag="PLW95_02000"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HOV21439.1"
                     /translation="MKEITYRQALNEAFDEEMMRDEKVFIFGEDVAIYGGAYGVTLGL
                     LEKYGDTRVIDTPISENAIVGTALGAALTGMRPVAEIMYIDFIGLCMDQLNNQVAKIR
                     YMFGGKCTVPLTIRTEGGAGRTLGAHHSQSLESWLIHIPGIKVVMPSTPYDAKGLLKS
                     SIRDDNPVVFIEHKMLYNTKGPVPEEEYTIPIGVADVKKEGKDVTIFTYSRMTLFSLQ
                     AAEELEKEGISVEVVDLRTLLPMDIETMVNSVKKTNKVVIVEEDCKTGGTGAEIGMQI
                     IENAFDYLDAPIIRVAGADVPMPKSKVLEQLAIPDVKRIKEGVKEVLK"
     gene            40651..41826
                     /locus_tag="PLW95_02005"
     CDS             40651..41826
                     /locus_tag="PLW95_02005"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOV21440.1"
                     /translation="MIREIIMPKLGETMEEGYLVSWKKQEGEKVEKGEVLFEVMSDKT
                     NFEVESPYSGYLRKILFSSSDEAIPVTTVIGYISDNPDEQLPEKEEKIEKKEVKEEKV
                     EIVQKEKVEEKIVEGGEKIKATPVAKRIAQERGIDISNIRGTGPGGRIEKRDVEKYIE
                     SKAVGVGEYEIIKWTPIRKIIAKRLTESKREIPHYYFQGKFIMDQIALIKELKKKEGN
                     DFSYTDFLLFFAGKAIEKYPLIDASVENDEIRVYKSVDIGLAVAVETGLVVPVIRNVC
                     QKTLEEISEERKVIAKKSKEGKLEENDLKGARFVISNLGMYGVENFQPIINPPGVAIM
                     GVGKIEKGIIVIEDKIEVKRIMSVSFSFDHRVIDGSYAGSFYNYFKEILENPSILVL"
     gene            42060..42794
                     /gene="cysE"
                     /locus_tag="PLW95_02010"
     CDS             42060..42794
                     /gene="cysE"
                     /locus_tag="PLW95_02010"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011406050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HOV21441.1"
                     /translation="MIGRIKEDINTVFEKDPAARNIFEVITSYPGLHALWGHRFAHFL
                     WKRNMKTFARIISNVNRFFTQIEIHPGAEIGRRFFIDHGCGVVIGETTEIGNDVLLYQ
                     GVVLGGITHEKKKRHPTIGNNVVIGAGAIVLGPVKVGDGAKIGAGSVVVKDVPSDSTV
                     VGVPGRVVKQQKKRKIELDHSNLPDPVADALKLVIDEIEKHEERIIKLESVEGIKAKI
                     DEYFAMKKEEISEIFSKIKENENEKN"
     gene            42781..43866
                     /locus_tag="PLW95_02015"
     CDS             42781..43866
                     /locus_tag="PLW95_02015"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOV21442.1"
                     /translation="MKKINILFLSSLFIFILSFSGYCEIVKVYHKVKRGETLIAIAKK
                     YGTSVEKIKELNGLKTSLIKEGQKLVVKKIEKEENKNLSSNKESLTTSSGNYDTVYYV
                     VKKGDNLSKISRKYDISVSSIKKENNLKSSKIIPGMQLKIRVPVEVPKIENIEPIIGT
                     EQKDFYQVKKGDTLEEIAKKFSVLPEELKKYNLLRDNDFKEGQIIIIPQQEQLTSNPE
                     SQTNSETDIEKEQLTRAKILDIAFSYLNMPYKLGGNGEKCIDCSTLTRLVYEKIGFEL
                     PKTSYQQAKLGKEVTLNEALPGDLVFFRRGIGIGHVGIYIGNKLFIHASSSDRKVKID
                     SLENSYFKSHFVCLKRYIPFSSSVFGG"
     gene            43869..44891
                     /locus_tag="PLW95_02020"
     CDS             43869..44891
                     /locus_tag="PLW95_02020"
                     /inference="COORDINATES: protein motif:HMM:NF025839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4432 family protein"
                     /protein_id="HOV21443.1"
                     /translation="MMSRNSNCRCCEYLWNSLKIVVIENEILRIEILADKGTDIVEFL
                     YKPEDIDFMWRSPIPLYNRFMFPITKELDSGNFIDFYPGGWQEIFPNGSSPCEYKGTV
                     LGLHGEIALLPWDYKIIEDSSEKISVLFSVKTYRTPFYIEKIISIEKEKPYIEIEEKV
                     KNIGGEDMDFIWGHHPAIGKPFLSSDCFIKIPECKIKVVHGDGKSITNLKQTEGIWPY
                     LEGIDRNKVDISKIPSENDCVSDMMFLTDLKEGKYEIVNKKRKLSFFFEFPLSVFKCL
                     WFWRIAKGSFNYPWYGRNYNIALEPFSSLPNLAEAIKRGDQLKLKPDEEMRVKIKAGV
                     IKEENG"
     gene            44884..46302
                     /gene="gatB"
                     /locus_tag="PLW95_02025"
     CDS             44884..46302
                     /gene="gatB"
                     /locus_tag="PLW95_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012446948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOV21444.1"
                     /translation="MGEYEVVIGLEVHVELSTCSKMFCPCSTEFGASPNTNICPICIG
                     MPGTLPVINEKAIECAVKTGIAFNSDIARTCRFARKNYFYPDLPKNYQISQYEQPIIK
                     GGYLKVRDKKINLTRIHLEEDAGKLIHSEEGENFSYIDYNRGGIPLIEIVTQPDITSP
                     EEGEEFLLKLKQTLKYLEVSDCNMEEGSLRCDANISVRKKGDEKFGVKVEIKNMNSFK
                     AVRKALSYEQKRQISILNRGGKIQQETRLWDDKLQKTEIMRTKEEAHDYRYFPDPDLL
                     PVEIDDEFINKVKEEIGELPDDRKERIKVQYGLPTYDIDVLMSEKIIIDYFEECSKIF
                     KNYKLISNWIMGEIMSLLKGKEIKFLFEKIPPEKFIDLLKMVEEGKITSTVGKNVLKE
                     ILKTGKFPSEIVKEKGLLQIGDEEFIENIVKQVIDENQKAVDDYKKGKEKVMAFLIGQ
                     VMKKTKGKVNSEIVEKTIKKLI"
     gene            46379..47566
                     /locus_tag="PLW95_02030"
     CDS             46379..47566
                     /locus_tag="PLW95_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOV21445.1"
                     /translation="MRKKFFLTIIGFFLLTIILGSDVKVSEERNKKEETYKNIELFID
                     ALNIVEKNYVKEVENKDLIYGALKGMLMSLDPYSAFLPPDLKKELQIETEGQFEGVGM
                     EITLKNGIITVVSPIEDSPAWKAGVKPGDRIITIDGESTKGMTTLEAAKKLRGEKGTK
                     VKISVMRENMPKLIDIEITRDIIKVKSVKTEDIGEMGYIRITEFQERTSSDLKNALEE
                     FNKKEKKGIILDLRNNPGGLLTSAVEVSELFLQKGKLIVYTQGRNKEDKSIFRSKKVP
                     IWEKPIVLLVNQGSASASEIVFGALKDNYEKISSVGEKTFGKGSVQNLIPLSDGSALK
                     LTIAYYYTPSNVCIEGEGIPVDYEVKLPEDKEIIFATDEDVQFQEAKRILKNMIYEKD
                     EKE"
     gene            47544..48401
                     /locus_tag="PLW95_02035"
     CDS             47544..48401
                     /locus_tag="PLW95_02035"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HOV21446.1"
                     /translation="MRKMKKSDKKETSWLVPIVLLLILGILITGVKVLNEIYKKEEIP
                     GKKIPAKKEKVVEKKEKKREKEIPVAIIIDDCGWNRSIVNELEGINEPLTLSFLPKAP
                     YSKELIKLLNGKDYEFILHLPLEPLPPQQCLDKGLITTEMTDEEINKIFLDNVYDYLP
                     YIKGINNHMGSLFTSTPDKMEVLLENIKKENLFFIDSATSKNTTGYTLAEQMGIKTAK
                     RDIFLDNESNPEYIKEQLNKLIKVAKKNGKCVAIGHAKKETLKVLKEKLPELKEEGIK
                     IVPASALVE"
     gene            48446..49429
                     /gene="tsaD"
                     /locus_tag="PLW95_02040"
     CDS             48446..49429
                     /gene="tsaD"
                     /locus_tag="PLW95_02040"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOV21447.1"
                     /translation="MIIIGIETSCDETSIGILENNKLLANIVASQEDIHSKFGGVVPE
                     LASRQHLEKIDILFNIAVEKTKININNINYIGVTSQPGLKGSLIVGVSFASGLSFSLK
                     KPIYKIDHLKAHISANYFDENIEFPAIGLVISGGHTSFYYLKNHIDFELLGRTLDDAC
                     GEVFDKVGRMLALPFPGGPQIEKNAKEGDENKIKFPVALISKNSLDFSFSGLKTAVNY
                     HIKKNGLNNIPDICASFQKVVADTLCIKIERAINRYKVKSLLLGGGVVQNEYIRGRIK
                     EKIGEKIKLFIPEKKLCMDNGGMVAILTYFLIKYGVKPSENKIEVIPTVEI"
BASE COUNT        19744 a         5274 c        10218 g        14251 t
ORIGIN      
        1 atccagttaa ttttccatct tcatttttaa ttgtatttat tactttaagc aattttgctt
       61 cttcatcaac ttcattaaca agaaaaaaag catctttttt atgcggtatt gttaaattta
      121 cccctttaaa attaaaaaat ttaattgctt caattccttt tttaagaaaa gatggtttta
      181 tttcaaatgg aatataaaca aaatcaagag aaagatattg aaaagcagca ttgtgaaaaa
      241 taggggaaag agaatgagac actggatttc caaagacacc tgtaattttt gtagttcctt
      301 ttatcatttt ttcctctgct taattttagc atattattga taataaaacc aaattttgtt
      361 ataatacaat acgaaaagcg ggagtagctc agcggtagag cataaccttg ccaaggttgg
      421 ggccgcgggt tcaaatcccg tctcccgctc tatctctaaa tttgtaaggg agttgaacct
      481 tttgggttca aaccgacaaa agtagcgagg attggtaagg cagagttggt gcggcatcgt
      541 tttccagata gcacaactgg ggttatggga gatttttcta aatccacccc atccctcctt
      601 ttttaaagga gggagtaatt tggtgcgtta tcgtctttca gatagcacaa ctgctaccga
      661 caaaagtagc aaagattaga ggttatgttc tcttttattt gaataagtta tatgatataa
      721 ttctaacctt tataaacagt aaagataaat ttttatttaa aaaaatggga aagaatattg
      781 ttaattattc aagtgaaaaa agatttgcta aaattcagtc attaaaagat aaagaagtat
      841 tttttattga tttagatggc acagtttata taggaaataa aattgttgat ggagcagtag
      901 attttgtaaa tcaactttat gaaaatggga aattatattt ttttatttct aataattctt
      961 ctaaatcaaa agaggactat gtaaaaaaaa taagaagata tggtataaat gttaaagaaa
     1021 agcaaataat tctttcaact gatgggttaa ttgatttttt aaaaagtaat gaaattaaaa
     1081 atgtttttac aataggaaca gagtcattta aaaaagaaat taaagataat gaaattaatc
     1141 cggagtcaaa aaatcctgaa tttgtagttt taggttatga tacagaactt acatataaaa
     1201 aattggtcaa aggaatatat tatgtaaata atggagttga gtacatagcc actcattgtg
     1261 atattgtttg tcctacagaa aatgggccag ttcctgatgt aggaacatta attgaaacat
     1321 ttaaactcac aacaaaaaag ggcccattta agatatttgg gaaaccaaat aaagaaatga
     1381 tagaatttaa gataaaagaa ttaaaagttg atataaataa agttgtattg attggagaca
     1441 ggttatatac tgatggagta cttgcaaaga atataggagt aacatatatt ttgccacttt
     1501 caggtgaaac aaatagagaa atgcttgaaa gttctaatgt attaccagat ttaattgtag
     1561 aaaaattagg tgatttagta aaatatgttt aaaaaatata tttttaaaga agtaaaatga
     1621 aaaaatactt ttagttttac attaacaaat ttatattgac agaatttttt aaaatgttat
     1681 aactaatgag ggaatacaat tttctaaatg ttgctgctgt ggctcagtgg tagagcacgt
     1741 ccttggtaag gacggggtca tgggttcaaa tcccatcagc agcttaaaaa cataaggagg
     1801 gaataaaatg gcaaaggaga aatttataag gagcaagaca catgtaaatg tagggacgat
     1861 agggcatgta gatcatggga agacgacatt aacgagtgcg ataacgaagg tattggcgaa
     1921 ggagaacaag gcaaaggaga tgaagtatga ggagatagac aaggcgcctg aggagaagga
     1981 gaggggatta acgataaaca tatcgcatat agagtatgag acagagacga ggcattatgc
     2041 gcatatagat tgtcctgggc atgctgatta cataaagaac atgataacag gtgcggcaca
     2101 gatggatggg gcgatattgg tagtaagtgc gccagatggt cctatgccac agaccaggga
     2161 gcatatatta ttagccaggc aggtagaggt gccggcggtg gtagtatttt tgaacaagat
     2221 ggatagtgta gaggatgagg aattagttga gttagtggag gtagaggtaa gggagttatt
     2281 aagcaagtat gggtttccag gtgaggaggt acctgtaata aaggggagtg cattgaaggc
     2341 attggagtgt ggatgtggta agagggaatg tgagtggtgt gggaggatat gggatttatt
     2401 agatgcagta gacaggtaca taccgatgcc ggtgagagat atagacaagc catttttgtt
     2461 ggcagtagag gatgtattta gtataacagg caggggtaca gtagcgacag gaaggataga
     2521 gaggggtaaa gtaagagtag gggataatgt agagatagta gggttaaagc atgagataac
     2581 taagacggta gtgacagggg tagagatgtt caggaaggaa ttagaagaag gggtagcagg
     2641 agacaatgta ggtttattat taagaggtat agataaggat gcgatagaga ggggtatggt
     2701 agtggcggcg ccagggagta taacaccgca cacacatttt aaggcacagg tatatgtatt
     2761 gaagaaagaa gaaggtggca ggcacacgcc atttttccat ggatacaggc cgcagtttta
     2821 cataaggacg acagatgtaa caggggagat aaagttacca gagggggttg agatggtaat
     2881 gccaggagat aatatagaga tggatataaa gttaatatat ccagtagcac ttgaaaaggg
     2941 gcagagattt gctataagag aaggtggaaa aacagtcggt gctggtgctg ttactgaaat
     3001 tattgaatag ggtgaataag aaatgagaaa tataataact ttagtttgtt cagaatgtgg
     3061 aaataaaaat tattacacta caaagaaaaa acaaacagga cagaagaaga aaattgaatt
     3121 aaaaaaattt tgtcctaaat gtgggaagca tgttctccat aaagagggta aataaaattt
     3181 gacaaaaata tttgatatta ttaaattaaa tggtataata atatttttgt aaaatctatt
     3241 tacctgaaaa atattttttg aatttataaa tgtttggtat tttattcgtg ttattgacat
     3301 atatataaaa agtggtataa taaattattt ataggcctgt agctctaatt gggagagcaa
     3361 cggactccaa atccgagggt tggaggttca agtcctccca ggcctgaaca agtaattaaa
     3421 tgaaaaagaa aatatctgat ttttttaatg aagtaaaggc agagttaaaa aaagttacct
     3481 ggcctgaaaa aaaaatgtta aagagaacaa cagttattat tgttattttt attttcataa
     3541 tggctcttta tattggaatt attgacctga tattttcaaa agtgattatt ttatttttga
     3601 ggtaattatg ggaaaatggt atattttaca tgttagaaca gggcaggaag agaaaattag
     3661 aaaacttctt gagaatcaac ttaataagaa agaaaaacaa attacacaaa tagttatccc
     3721 aaaggaagaa atagttgaaa gaggaaaaga ggagaaaaaa acaacaagaa gattctggcc
     3781 tggatatatg ttaattgaaa tagcgcctga atttgataat gaaattttat ggcacagaat
     3841 aaggacaacg acaggagtac taaattttct tggtgcaggt gataagccag ttcctttaca
     3901 accagaagaa gcaaaaaatg ttataggaga agttgaaaaa agaaaaagca aaccaactcc
     3961 gaaagttgag tttaaagaag gagataaagt tgaaataaca gaaggtccat ttatgaattt
     4021 tacagcaatt gttgaggagg tttatcctga taagggacga cttaaagtaa gtgtctctgc
     4081 ttttggaaga agtactcctc ttgaacttag tttctggcag gttgaaaaaa tataggagtg
     4141 aaaatggtag aaaagaaaat caaagcagtt gttaaacttc aaataccagc aggaaaagca
     4201 actccagcac cacctgttgg accggcactt ggtcaacatg gtgtaaatat tatggaattt
     4261 tgtaaggcat ttaatgatgt gacaaaagga aaagaaggaa cagtccctgt tgttttaaca
     4321 atttatgaag ataaaacatt taattttata ataaaaacac ctccaacttc cgaattaata
     4381 aaaaaatacg caaatattgt aaagggttca gcaattccta atagagaaaa aattggcaaa
     4441 ataacaagaa aacaaattga ggaaattgca aaaattaaat tacccgactt aaatacaaaa
     4501 gatataaaag aagcaatgaa aattgttgag ggttctgcaa aaagcatggg tattgaaatt
     4561 attgattaga caggaggaaa aattgggtaa aaggtattct actttattag aaaaagcaga
     4621 aaaagataaa ctttatacga tagaagaagc aatagataaa ttgaaagaaa tgcattctgt
     4681 gaaatttgat gaaacagttg aattatctat aaaattaggt atagacccga aaaaattgca
     4741 acaaccaata agaggaagtg ttgtttttcc atatgggata gggaaggaag taaaaatact
     4801 agtttttgca acaggtgaat tagcagaaaa agcaaaaaat gcaggagcag attatgttgg
     4861 tggaaacgaa cttgtagaaa aaataaaaag tggctggttg gactttgatg cagttgtttc
     4921 tactcctgat atgatgaaag ttgtttcacc cttaggtaaa atacttggtc caagagggtt
     4981 aatgccaaat ccaaaaacag gaactgttac taataatgta gaagaaatag ttaaggaatt
     5041 aaaaaaggga agaattgatt ataaaatgga taaagaggga aatattcaaa ttccatgtgg
     5101 gaaaatttct tttgataaag agaaattggt agaaaatatt attgaggcaa ttaagaatgt
     5161 gttaagcgtt aaaccatctt ctgttaaagg aatatatata aacaatatgt atttatcttt
     5221 aactatgtcc ccttctttaa aacttaattc taccaggata ttacaagaat taaaaatttc
     5281 ctgagagaat gaatactttc gagttccatg ggaagtaagg agtggagagt agcaagatag
     5341 aaaaacaaat aaaaaacaag gaggatacag gggttaagtt tttttcctta ctttttactt
     5401 gtaacttgct gattaaatag agagagcgaa gtaaatattc atataagggg tgtaaaatga
     5461 aaaaaatgaa caagaataag aaaaaagaaa ttgtaaattg gttgacagaa gaatttaaaa
     5521 atagtaaaat atatctttta gttaactttt caggactttc tgttttagaa atgcagaatt
     5581 taagacaact tataaaagaa aataatggtg atataaaagt tataaaatca agtttaattg
     5641 aaagaacatt taagaattta agtaaagatg aagcagttaa ttatttgggt ggtccaattt
     5701 ttgttgtatg gacaaaagat ggggatgaaa ttgaaataat aaaaaaactt tatgccttcg
     5761 ggaaaagggt tggtaaaata gcagtaaaag gtggagtaat aaataataaa ataagtgatg
     5821 cagaattttt tgaaaaatta agtaaacttc cttcaataaa agaaattcaa agtgaactta
     5881 tatatgcaat aaaattccct gttattagaa ttaccaattt aatgagaagt cctgtggtaa
     5941 gaattgtaaa tatattaaac caaatatctc aaaaataaat caaaaaggag aaagaaatgg
     6001 ctgaggaaaa aaatgtattg gatgagattg ttgagaaagt tgaaaaactt acagcgattg
     6061 agttgtcgga attggcaaca aaattagaag agaagtttgg agttaaaggt atggtgggtc
     6121 ctgttgcagt gtcatctgta ccaggtgttg caggacagga agaaaagaaa gaagaaaaga
     6181 caacttttga tgtaatttta aagggatttg gaagtaataa gatacagaca ataaaagaag
     6241 taagggcaat tacaaatttg ggattgaaag aagcaaaaga attggttgaa tcagctccaa
     6301 aaccaataaa agaaaaaatt tcaaaagaag aagcagaaga aataaagaag aaattggaaa
     6361 cagtgggggc agaagtagaa ataaaataaa aaaaacttta tagagggaga aaataaatat
     6421 ggaaagaaaa ggtagtagtt tttctttttt agaagaaaaa aatgagttta ataacaaagt
     6481 ggtagaaaat gttgaaatac cagacctcct tcttgttcag aaaaaatcat atgaagaatt
     6541 tttacagcaa aatattcttc ctggaaaaag agaagttaaa ggaattgaag aagttttaag
     6601 aaaaattttc cctattgaaa gtaaaaatgg gaatttaata cttgaatatg ttaattatac
     6661 tattgagttg cctgtttatt cagttgagga atgtattgaa aatggactga cttatgatgg
     6721 aaaatttaaa ataaaagttc gtcttgctat aaaaaatcat gagacaggga aaattatttc
     6781 ggttaaagaa caggaagtat atattggaag tattccattg atgactgaaa gtggtagttt
     6841 tattataaat ggaattgaaa gagtagttgt aaatcaactt ttaaggtgcc ctggtgttta
     6901 ttttaaagaa gaagaaataa atggccaaaa aacattatat tctgccaagg tatatccttt
     6961 acgcggctta tggatagagt tccacattga tagccataat tgtcttatcc tttttcttgg
     7021 gaggaaaaaa gttatcggga caatgtttct caaagcattg ggtttaacaa atgaagatat
     7081 tgttaaggag ttttatggtt cagaggagaa aatgcctgaa aattcaattc taagacagac
     7141 attactgaga gatacaacaa agaccagaga agatgcctta aaagaaattt ataaagaact
     7201 aagacctggt tatcctccaa tcattaaaga agcggaaagt ttttttaatt ctctcttttt
     7261 ttcagatgaa ttatatagtt tgtcagaagc aggaagggca cagataaata gaaaattaaa
     7321 ccttaatctt acacaacagc atttaacaaa agaagatata attgggataa taaagtatct
     7381 tttaaatctt actgaaaaag gtgaggggac aactgatgat attgaccatt taggaaacaa
     7441 aagagtaaga acaacaggtg aaatagtaaa ggaacatctt tatgaaggtt ttttaagaat
     7501 gcgaaggtat tgtgaagaga agatgaaaat tattcctccc gaagaagttg ataagaaagt
     7561 tactccacag gactttataa atggtaaagt tttaatgact gttttaaatg atttttttgc
     7621 aagaaaccaa ctttcgcaat ttcttgagaa aataaatcca ctctcagaaa taactcataa
     7681 aagaagagtt acagcagtgg gagaaggtgg aataaaagaa agaaaaaggg ctggttttga
     7741 agtgagagat gtacattata cacattttgg gaaaatatgc cctattgaaa caccagaagg
     7801 agcaaatata ggactaattg gttctttaac ggttcattca agaatagggg aacttggatt
     7861 tatagtaact ccatatttta aaattgaaaa tgggaaagtt acagataaaa ttgaatattt
     7921 aaatgcaaat gatgaagaga attttaaaat tgctcctcct gatgttgagg tggacccaaa
     7981 aacaaaggaa ataaaagaag aagtagtaaa agttagagta aaagatggtt tatttgaagt
     8041 tgtcccaaaa aatgaggttg attatatagg ggtttctcct attcaaatag ttggtgttac
     8101 tgcatctctt ataccattcc ttgaacatga tgattcaaac agagcactta tgggttcaaa
     8161 tatgcaaaga caaggagttc cacttattaa aagtgaagtt cctcttgtaa aaacagggat
     8221 ggaaagattt gttataagag atagcgggtc tgttgtaaaa gtaagaagtg atggaattgt
     8281 taaatatgtt agtgcagaaa aaattgttat tgagaaacag gaaaaaggta ttttcccatg
     8341 ggaaaaagaa gatatttatt atttgaagaa atttacgaga accaatcagg atacatgttt
     8401 tcatcaaaga cctgttgttg atattgggga cagagttaag aaaggacaaa ttatttcaga
     8461 tggtctagca acttcaaaag aaggagagct tgcattagga aaaaatttat tagttgcttt
     8521 tatgccctgg catggatata actatgaaga tgcaattatt ttaagtgaga gaatggttaa
     8581 agaagatgtt tttacttcta ttcacataag ggaatttcca gttgaagcaa gagaaacaga
     8641 attaggtcct gaagaaataa ctgctgacct accaaatgtt ccagaatccc ggctaaaaaa
     8701 ccttgataaa aatggaataa taagagttgg agccgaagtt gaagcagatg atattcttgt
     8761 tgggaaaatt actccaagag gtgaatctga attaactcct gaagaaaaac ttttaagggt
     8821 aatatttggt gaaaaagcga aggatgttca gaatacatct ttaaaagttc caccaggaat
     8881 taaaggagtt gttgttaatg ttaggaaata tgaaagaaga gaaaatataa aagaagaaga
     8941 tataaaaaag gaacttaata aagtaaataa ggaatataaa gaaaaggaaa aaataataaa
     9001 agagattata aaagagcaaa tagaaaaaac tcttaaatta cataatataa aggcaaaagt
     9061 tacaacaaca aaagagacat ggaataaaat agttgaaaaa atacaggatg aaaaagtaaa
     9121 agagaaatta cagaaattaa tagatataga aaacgaagaa attaaaaaat taaagaagga
     9181 actaagagat aaagaaaata aaataaaaaa aggtcctgaa cttgaaatgg atgttatata
     9241 taaaattgtt gttgaaattg cagttaaaaa gaaagtatct gttggtgata aaatgagtgg
     9301 tagacatgga aataaaggag ttatatcaaa aattttgcca gaagaagata tgccttttat
     9361 agaagatgga acacccgttg atattatatt aaatccctta ggtgttccat caagaatgaa
     9421 tattgggcag attcttgaaa cacatttagg cattgcatta aaaacgcttg gatatagtta
     9481 tgaaacacca atatttaaaa gtataaaaga attggaaatt aaagaactac ttaaaaaagg
     9541 tggactatca gaaaatggga aaatgacttt atatgatggc cagacaggag aaaagttttc
     9601 tcagccaatt actgttggtt atatttatat gatgaaattg attcacttag cagatgaaaa
     9661 aattcatgca agagcaactg gttcatattc acttataact caacaaccat tgggagggag
     9721 aagccaattt ggaggacagc gtttaggaga aatggaggtc tgggcacttg agggatatgg
     9781 agcagcacat acgcttaaag aaatgcttac tgtaaaaagt gatgatactg aaggaagaaa
     9841 acaaatatac gagagtattg caaaaggtat tgatttcaaa tcaaataata ctcctgaatc
     9901 gtttaatgta ttaaaaagag aattacaggg acttggattt gatttgagag ttgaaaaaag
     9961 aaatattggg ggtaaatcaa aggaaattgt aacaataaaa gttgcttctc caactgtaat
    10021 gcgttcatgg tctcatggtg aagttaaaaa agcagaaaca cttaattaca gaacattgag
    10081 gcctgaaaga gaagggttat tctgtgagag aatatttggt ccaacaaagg attatgaatg
    10141 tgcatgtggt aaatataaaa aattaagata caagggaata atatgcgata gatgtggtgt
    10201 tgaagtaaca acaaaaagag taagaagaga aagaatggga catattgagc ttgccactcc
    10261 tgtatcttat gtatggcttt ttaaaagttc atctaattgg atgggaatgt tacttgacct
    10321 atctcaaagt gaacttgaaa tggtttttta ttatgcgaga tatatagtta tagaccctgg
    10381 taaaacaaac ctgactaaaa aacaattatt aacagaatca gaatattatg aaatgactga
    10441 aaaacatgga aatgcatttc gtgcagaaat tggtgcagag gcaatttttc accttttgaa
    10501 agaaatagac cttatatctc ttgaagaaca gataaagcag tcattgagta aaatgaaaaa
    10561 aaacgagcgt gaaacaaaaa gaaaacttat taaaaaatta aggatgatcc agggactttt
    10621 aaagacgaat actaaaccag aatatcttgt tacaaatatt atacctgtta tacctcctga
    10681 tttaaggcca ttacttccac ttgatggagg gaaatttgtt gcttctgacc tgaatgacct
    10741 ttaccaaaga gttataaaca gaaataatcg tctaaagaaa cttataaaat tgcaggcacc
    10801 ggatattatc ataagaaatg agaaaaggat gcttcaagaa tcagtggatg ctttaatgga
    10861 taatgggaaa catggaaata ctattcttgg aaaagggaga agacctttaa aatcacttgc
    10921 agatgcatta agaggtaagc aaggtagatt cagacagaac cttttgggca agagagttga
    10981 ttattcagga agagcggtta ttgctgttgg ttctgaatta aaattaaatg agtgtggtgt
    11041 tccaaaagaa atggctcttg aactttttag tccatttgtt ttaagagaat tgaggaaaaa
    11101 agaatatttc cacacacttg gtagtgcaaa aagagcaata agagagaata aagaggaggt
    11161 ctgggaaata cttgaaaggg ttagtaaaca tcatccagtt cttcttaata gacaaccaac
    11221 tttgcaccga ctgagtatcc aggcatttga accacggctt atagagggta atgtacttaa
    11281 actccatcct cttgtttgtt cagcatttaa tgctgatttt gatggagata caatgagtat
    11341 tcatattcca ctattgccag aaacaatatt agaagcagaa cttttaatga aggcaacaac
    11401 tcatatactt tctccagcca gtggtaaacc aattgtttct ccatcaagag atattggtat
    11461 gggttgtttc tatcttactt ttattagtga tgaagaagaa tatcctgaag atgagagaaa
    11521 atatatttca agttttgaag aagcaaaatt tctttaccat aatgggtttg ttgaattgca
    11581 cagacctatt agagtgagag atgaaatagg agaaattatt acaacaactg tcggaagaat
    11641 tctttttaat gaaattcttc cgtttgaatt gccttttaaa aatgaggaat ttgaagtaaa
    11701 gaagttagaa tctcttataa aagaaatatt taataaattt ggctatgaaa aaacagtaga
    11761 agttcttgat ggacttaaag aaattggatt tttatattca acaattggag gaattacaat
    11821 aggtatagat gaccttaaga tacccgaaaa gaaaaaagat attattgaaa gagcaacaaa
    11881 agaagcagaa aaagttgaaa gaaattataa aaaaggaatt ataacagagg atgaaagata
    11941 taatagaatt gtggatatat ggacctctac cacaaatgaa gtttgcgatt atttaatgga
    12001 tacaattgaa aaagatagaa gtataaagcc atttagaatc aatccaatat atttgatggt
    12061 aacatcaggt gcaagaggta ataagaatca ggtaaatcag ttgatgggaa tgagaggact
    12121 gatgataaga cctactaaaa aagttacagg aggtattgga gaaattgttg aaacccctgt
    12181 tatatcaaat tttagagaag gtttggacct tcttgaatat tttacatcaa tacatggagg
    12241 tagaaaaggg cttgttgaca ctgctttgaa gacatctgat gctggatatt tgagtagaag
    12301 gttagttgat gttgctcatt ctgttatagt cacagaagaa gattgtggaa cgcttgaagg
    12361 tatccttata ggtcctcttg ttgaagaaga tgaagtaata ataccattag aagaaaggat
    12421 aattggtaga gttgcagtag ataatgttgt tgatattgtt actgatgagg aaattgtaag
    12481 agcaggtgaa gttattgatg aagaaaaggc aaaacgtctt gttgaggcag gaatacagag
    12541 aataagaata aggtcagttc tcacttgtaa agcagaaaaa ggtgtttgtg ctaaatgtta
    12601 tggctgggat ttgtcaagag gaatagttgt taatatagga gaggcagtag gagttgttgc
    12661 agcgcaatca attggagagc cagggacaca acttacatta aggacattcc atactggggg
    12721 gactgcttca agaggaattg gttcttctca attcactgtt aaaaaagatg gaatattgaa
    12781 atatgaagat aatttaaaaa cagttatgaa taaagaaggt aaaatggtag ttgttcatag
    12841 agaagggaat gtaaaaattg ttgatgaaag aggcagagaa tttgaaaaat atacattaag
    12901 agttggttct attattgaat tgaaagatgg aagtaaagtt aaagaaggac aacttatagc
    12961 aagttggaat ccatattcaa cacctataat tactgataag gaaggattta taaaatatga
    13021 agacatagaa attggaaaaa cagcaaataa agaaatgatt ataatacctc acaaagaaga
    13081 taaagacccg agaataattt tgagagataa aaaaaataat attattgatg ggtatcatat
    13141 tccagaagga gcaagaattg ttgtaaaaga aggtgaaaaa gtagagccag gtgatattat
    13201 tgctgagaaa gttcatacta ctgcaagggc acaggatatt actgctggac ttccgagggt
    13261 ctctgaacta tttgaagcaa gagttcccag gaatcctgct gtattgagtg aaatagatgg
    13321 tgaggttgaa ataatgcctt ctgaaaaagg aacaagaaaa ataaaggtaa aaaatgagga
    13381 gacacaaaca gagaaaatct atataattcc ttatggaaaa catcttcttg ttggaaatgg
    13441 tgattatgtt attgctggaa gaaagttaac tgatggacct gtggtgctac aggatatttt
    13501 aaaaatacag ggagagaaaa aagtccagga gtatctacta aacgaaattc aaaaggtcta
    13561 tagagtagaa ggtgttacaa taagtgataa acatatagaa ataattgtca gacagatgct
    13621 ttcaaaagta aaagttgaag atgcagggga tacctatctt cttgaaggag aagaaatcag
    13681 tagagttgaa atacaaaaaa taaatgaatc attaccaaaa gggaaaaacc cagcaacata
    13741 taaatcattg gttttaggaa ttacaagagt tgcattaagt agttccagtt ttatttctgc
    13801 ttcttcattt caagaaacaa tgaaagttct gacaaattca gcaattcttg gagcagaaga
    13861 ttatttagaa ggtttaaaag aaaatgtaat tttaggaaga ttaattccag cgggaacagg
    13921 taaattcaca aaagaaatta cagaagagaa agctgaattt attgaaacac aaaagtaaaa
    13981 agggaggaaa tgatgccaac agttaaccaa ttgataagaa aaaaaagaag attaagaagg
    14041 aaaaaaagta aatcaactgc tttacaaagt aatcctttta agaaaggtgt atgtctttat
    14101 gttaagacaa tgacaccaaa gaaaccaaat tctgcactca gaaaaatagc aaaagttcgt
    14161 ctttcaaatg gtaaagaagt aattgcttat gttcctggta ttgggcataa tttacaggaa
    14221 cactcaatag ttcttgtaag aggcggaaga gttagggatt taccaggtgt taaatatcat
    14281 atagtgaggg ggacatttga tgcgggggga gttgagggaa gaaaaaaggc aaggtcaaaa
    14341 tatggtgcaa aaaaaccaaa agaagaagag aaaaaacagg agggttaaat gccaagaagg
    14401 aaaagagtaa taaagaaaag aattgatgaa gacccaagat acaatgatta tgaaattgaa
    14461 aaatttataa ataaacttat gtgggatggg aaaaaagatt tagcatatag aattttttac
    14521 gatatgatgg accaaataaa agaaaaaatg aatcaggacc ctttacctgt ttttaaaaag
    14581 gcactaaata atgtgaaacc gaaattggaa gtaagaccga gaagaattgg aggagcaact
    14641 tatcaaattc ctattgaagt ttctcctcat agaggtttat ctttagcaat aagatggata
    14701 gttgaaaccg caagaagtaa aaaaggaaaa tcaatagttg atagacttgt tgaagaaata
    14761 ttagaggcgt ataaaggaga gggggcaagt ataaagaaaa gagacgaaac acacaaaatg
    14821 gctgaagcaa acagagcttt tgctcattat agatggtaga aatttcacat tttacctttt
    14881 agaggtcaat tttttatgga taaaataaga aatataggaa ttattgctca tattgatgct
    14941 gggaaaacca caacgactga aaggttcctt tattatacag gaaaaacaca caaaatagga
    15001 gaagttgatg aaggtactgc tgtcatggac tggatggaac aggaaaagga aagaggaata
    15061 acaataagtg ctgctgcaac aacatgttta tggaaaggat ataaaattaa tattattgat
    15121 actccaggac atgtggattt tacagcagaa gttgagaggt cattaagagt tcttgatgga
    15181 gcaataggtg ttttttgtgg tgttgcaggg gttcagtcac aatcagagac agtatggaga
    15241 cagagtgaaa aatataatgt ccctaaatta gtttttgtaa ataaattaga tagagtggga
    15301 gcagattttt ttagagttat tgatgaaata aaaattaaat tgaatacaag cccattaatt
    15361 atgaatatac ctatatatga tgacaatgaa gaatttattg gtcttattga tattattgag
    15421 gaaaaggaag ttatatataa tgagacatat gaaacagagg cagaagtaaa agatattgag
    15481 gaaaaaaata gagaaaaatt tttatattat aaaaagaatt taattgaaaa agtagcagag
    15541 gtagatgatt atttgatggg aaaatatgtt gaagaggaag aaataaaaaa aagtgagata
    15601 aaacgagcaa taaggaaagg gacaattaaa aatttatttt gccctgtatt ttgtgggtct
    15661 gcactaaaaa ataaagggac attattttta cttgatggaa ttgttaatta tctaccttca
    15721 cctgttgaca gaggtaaaat agaagggttt tctactgata agaaaaaaaa gattgaaagg
    15781 catccaaatg aaaatcaacc attttcaggt ctcattttta aggtatataa tgatattcat
    15841 ttgggcaggg ttttatatac aagaatttat tcaggcaaaa tcaaaaaagg ggaaaaagta
    15901 tataatagtt ccagaaatgt aaaggaaaaa cttatgagaa ttttagaagt ccatacaaat
    15961 aaatattatg aaaaagaaca gggaaaagca ggtgaaattg ttgctttact gggaccaaag
    16021 aaaacatata ctggggatac tatttgtgat gaattaagtc ctataatttt tgagagtgtt
    16081 gagtttcctg aaccggttat ttatttagca gttgaaccca aaacaatgag tcaacacgag
    16141 gatgtttata atgcactttt aaaaattgca gatgaagacc ctacaataaa aatcagaact
    16201 gatgctgaaa cagggcagat gataattatg ggaatgggtg aattacatct tgatgttgtt
    16261 attggaaggt taaaaagaga agataaactt gaagtaaagg taggcagacc ccatgttgcc
    16321 tatagagaaa caattacagt tccgtcaatt ggggaaggga aatttataaa gcaaagtggg
    16381 acaaaaggaa tatatgggca tgtagtatta aaattagagc cattagaaag aggcaaaaga
    16441 tttatttttg aatcaaaagt ggatagtgaa aaaattcctg atgaatttat acctgctatt
    16501 gaggaaggaa taaaagaagg tatggaagtt gggacattgg ggggttttcc tgtaattgat
    16561 attaaagcag ttgttattga tggttctttt catccaacgg attcagatga agttgcttat
    16621 aaaattgctt caacgattgc ttttaaagac gcatatagaa aaggatatcc tattctttta
    16681 gagccaataa tgaaagttga aattactgtt ccagaagaat ttttaggaga gattttagat
    16741 gatttaaatt ttagaaaagg gaaaatagaa agtattgaaa cacttaaaga tttaaaaatt
    16801 atagtagcaa gaataccgct caggaaaatt tttggttata caactactct tcgttccttg
    16861 acacagggaa aaggaagttg tataatagaa ccattatgtt atgaaagtgt taatgaagaa
    16921 attcttacaa aataataaag aatttcagta agaacctaaa tagaaaattt ttaccgaaat
    16981 ggagggaata aaatggcaaa ggagaaattt ataaggagca agacacatgt aaatgtaggg
    17041 acgatagggc atgtagatca tgggaagacg acattaacga gtgcgataac gaaggtattg
    17101 gcgaaggaga acaaggcaaa ggagatgaag tatgaggaga tagacaaggc gcctgaggag
    17161 aaggagaggg gattaacgat aaacatatcg catatagagt atgagacaga gacgaggcat
    17221 tatgcgcata tagattgtcc tgggcatgct gattacataa agaacatgat aacaggtgcg
    17281 gcacagatgg atggggcgat attggtagta agtgcgccag atggtcctat gccacagacc
    17341 agggagcata tattattagc caggcaggta gaggtgccgg cggtggtagt atttttgaac
    17401 aagatggata gtgtagagga tgaggaatta gttgagttag tggaggtaga ggtaagggag
    17461 ttattaagca agtatgggtt tccaggtgag gaggtacctg taataaaggg gagtgcattg
    17521 aaggcattgg agtgtggatg tggtaagagg gaatgtgagt ggtgtgggag gatatgggat
    17581 ttattagatg cagtagacag gtacataccg atgccggtga gagatataga caagccattt
    17641 ttgttggcag tagaggatgt atttagtata acaggcaggg gtacagtagc gacaggaagg
    17701 atagagaggg gtaaagtaag agtaggggat aatgtagaga tagtagggtt aaagcatgag
    17761 ataactaaga cggtagtgac aggggtagag atgttcagga aggaattaga agaaggggta
    17821 gcaggagaca atgtaggttt attattaaga ggtatagata aggatgcgat agagaggggt
    17881 atggtagtgg cggcgccagg gagtataaca ccgcacacac attttaaggc acaggtatat
    17941 gtattgaaga aagaagaagg tggcaggcac acgccatttt tccatggata caggccgcag
    18001 ttttacataa ggacgacaga tgtaacaggg gagataaagt taccagaggg ggttgagatg
    18061 gtaatgccag gagataatat agagatggat ataaagttaa tatatccagt agcacttgaa
    18121 aaggggcaga gatttgctat aagagaaggt ggaaaaacag tcggtgctgg tgctgttact
    18181 gaaattattg aataagtatt gacagaagag aataaaataa gtaaaataaa aaacttgacc
    18241 tgaaaagaga aaaatatgga agaaaaaaga attaaaatta cattaaaggg ttttgaccat
    18301 agattgttag ataaatcaat aaaagagatt attgaacttg ctaagaaaac aggagtaaga
    18361 atttcaggac ctgttccttt accaacaaaa agggaagttt atactgtttt aaggtcacct
    18421 catgtaaata aaaaaagcag agaacagttt gaaatgaaaa ttcataaaag attgatagaa
    18481 ataattgagc caactggaag aacaatagat gctttaacta aattaagtct accagagggt
    18541 attgatatta aaatggattt gagttaaagg atataataat gagaataggt ttaattggaa
    18601 agaaattggg gatgagcagg atttttaatg aaaatgggga tgttattcca gtgacgatta
    18661 ttgaagttaa agataattat gtaattggta taaaaaaaga aagcgagaaa gataattact
    18721 cagcaattca aattggttgc caggaagttg aagataaaaa acttagaaag tcagaattag
    18781 gtaagttaaa gaaaaataat ttaccatcat taaaagttat aaaagaactg agaatgaaaa
    18841 atgatgatga agttaatgac tttaaaatag ggcaaaaatt aaatgttgaa atttttaaaa
    18901 aaggtgattt agtggatatt actggtaaaa caataggaaa ggggtttcaa ggagttgtaa
    18961 agagacatgg atttaaagga ggtcccgcta cacacggttc tatgtctcat agaaaacctg
    19021 gttcaattgg tggtacaact ccggcaaggg tcctaaaagg acaaaaaatg gcaggacaca
    19081 tgggaaatga gaaacaaact attcaaaatt tagaagttgt tcaaataatg catgataaaa
    19141 atttaatttt aataaaaggg gctgttcctg gtcctaaaaa tagatattta ttagttagaa
    19201 aggcaataaa aggtcttagc aaatgagaaa aatccctatt tatgatatta atggtgaaat
    19261 tgttggtgag gaagaaattg aaatagaaaa tgaaaagata aataaagatg ttctctatta
    19321 ttatgttaat gcatatctta cgaatcagcg aaagggaaca tcttcaacta aaacaagggg
    19381 agatgtttct ggtggaggca agaaaccatg gaggcagaaa ggaactggac gggcgagagt
    19441 tggttcaatt agaaatccca tatggaaggg cggaggagtt gtttttggcc caaagacaaa
    19501 agaatataaa gttagattac ctaaaaaaat aaaaagaatg gcacttattg aagcaataaa
    19561 agataaaatt attgaagata gatttttact tatagaaatg agtaatttaa atgaaataaa
    19621 gacaaaaaat gtctataatt ttttgaagaa aataggtagt aataaggaaa aaattctttt
    19681 cattcttaat aaaggagatg taaataagga agttataata aaatcaatta gaaatataga
    19741 tactattgaa tatgattatt cggacaaaat aaatgcatat gaaattttaa aagttgataa
    19801 agttttttta gacgataaga atatttttgg agtaataaag aaaacattgg agaaagaaaa
    19861 tggcagttga agcgtataaa attataaaaa ttcctcttat atcagagaga gcaactgatt
    19921 tacaggcaga aaataagtat gtttttatag ttgataaaaa agcaaataag aatcaaataa
    19981 aagaagcaat agaaacaata tataaggttc atgttaaaaa aataaatgtg ataaatgttc
    20041 ctaaaaagaa aaagaaatat aggatgaata ttgaaggatt caggtctgga tataaaaaag
    20101 caattattaa gttaaaagaa ggagaaaaaa ttgccattac gtaaattaaa accggttaca
    20161 cctggacaga gacatgcaat tttagatgat ttttctgata taacaaaaaa gtatccggaa
    20221 aaatcacttg ttattggaaa aaggaaaaaa ggtggaagaa ataatactgg cagaattaca
    20281 gtaagacata gaggtggtgg acataaaaag ttatatagaa ttattgattt taaaggtaga
    20341 aaaaatatgg aagggaaagt tgtaagtatt gaatatgacc cgaatagaag tagcagaatt
    20401 gctttagttc actatactga tgggataaaa agttatctga ttgctcctta tggtcttaaa
    20461 gttggagata taattaattg tggggaaaat gcagaaatta aaacaggtaa tagattacct
    20521 ctgagaaata taccagaggg attacagatt tttaatattg agatggtacc ggaaaaaggt
    20581 ggacaaattg tcaggtctgc tgggactctt gcaactttaa tggtaaaagg agaaaaatat
    20641 gcgcaagttc ggttgccatc tggtgaagtg aggttgtttg atttgaattg ttttgcaaca
    20701 ttaggacagg ttagcaatat tgaccataaa tatgtttcct taggtaaagc aggaagaacc
    20761 agacatttag gaataagacc aactgtaaga ggagtagcaa tgaatcccgt tgaccatcct
    20821 catggaggtg gagagggaag aagtaaagga catcagtcac agagtccctg gggatggagt
    20881 acaaaaggtc acaaaacaag aaagaagaaa aaaccatcag ataaattaat tgtagaaaga
    20941 agaaaaagga gtaaaggatg agatctaaaa agaaaggtcc ctatgttgat ttgaaattga
    21001 tgaagaaagt tgaaaaatta agaagagaag gtagaaagga aataattaaa acatgggctc
    21061 gtgcatcatt tgttattcct gaatttgttg gttatacttt tgctattcat aatggtaaaa
    21121 attttttaaa tgtttatatt acagaaaaca tggtaggcca tagattagga gaattcgctc
    21181 caacaagagt ttttagaggc catggtgctc atactgaaag aacaagagag aaaacataat
    21241 aggatataaa aatggaagta aaagcaattt caaaatatat tagagtaagt ccgagaaagt
    21301 tgaaaatgat aaaggatatt gttattggga aagaaattaa tgaggcaata caaattttag
    21361 aaagtttgcc aaaaagaggt tcagaatatt tgattaaaac attaaaatca gcccagagta
    21421 atgcagaagg taaaaagaaa ggcggaatat ggatggtaaa gaatttattg gttgatagtg
    21481 gcccctcatt taaaaggttt agggctacta caatgggaag aggtgtaatg gtgagaaaaa
    21541 ggacttctca tataacagtt attttacagg atatagaaga aggggaaaaa taatgggaca
    21601 gaaagtaaat ccaattggat taagattagg aataactgag gattggcgaa cgaaatggtt
    21661 tgcaagtaaa aaggatttta aggactcatt gaaagaggat atttttataa gagacaatat
    21721 aaataaaaaa tatccaagag ggacaataag caaaattgaa attgaaaaag ttatcaaaga
    21781 aaaaataaaa attattattt atacaacaag accaggtgtt attttgggaa gaaaagggag
    21841 cgaaataaat caactaaggg atgagttaat tgggaagttg aaaaaacagg tgagtataga
    21901 tgtagttgaa gtaaatcctc caggtaaaag tgcacaattt cttgcagata caatagttat
    21961 acaattggaa aaaagatttt cacatagata tgcaataaaa agagcaatgg ctcttgcgat
    22021 ggaaagtgga gctgaaggaa ttaaggtaag aataagtgga agaatagggg gagtggaaat
    22081 ttccaggtct gaacaatata ttgaaggcaa agttccatta catacattaa gagcaaaaat
    22141 tgattatgca actgcgacag catttacgag gtcgggaaca attggtataa aggtatggtt
    22201 gtacttaggt gaagttttac agggtaaaag gcaggaggat aaaaatgccg ttaatgccaa
    22261 agagagttaa gtatagaaaa gcacaaagag gcagaagaaa aggtaaagca atgagaggca
    22321 gcactttata ttacggtgat tatggattga aaattatgga accgtgctgg cttacaaatg
    22381 ttcaaataga agcggcaaga gttgctatta cacaccattt aaaaacaaag gggaaagttt
    22441 ggattagaat ttttccggat aaaccagtta caaaaaaacc agcagaaaca agaatgggaa
    22501 agggtaaagg agatgtttct cactgggttg ctgttgtact tccaggaaga attgcttttg
    22561 agatagttgg agttgatgaa gaaacagctg tcggagcact taaaagagca tcctcaaaat
    22621 ttcctgtgaa aagtaaaata gttaaaagac aaaagatagt ttcataagga ataaaaatga
    22681 aaaaagatga aataaaaaaa tggagagaag caacagaaga agaaataggg cttaaaatag
    22741 ttgagttaga aaaacaactt tttaatttat taccacaggt aaaaatgggg cagttaaaaa
    22801 attgttctgt tttgaaaaat ataagaaaaa acattgctat tttaaacaca ataaagagag
    22861 aaaaaagtat acagagggtt aaaaaatgaa taattcacga gaaatcgaaa aagttggggt
    22921 tgttttaagt gataagatgg ataaaacaag agttgtttct gttgagtgga aatcagccca
    22981 tcctctttat aataaaattt taaggaagag aaagaaattt tatgcacatg atgaagataa
    23041 tattacaaaa gttggagata cggtcaggat tgttcagacc agaccattga gtaaactaaa
    23101 aagatggaga attgttgaaa taattgaaaa aacagaaagg caaaaatgat acaattgagg
    23161 actactttaa tagttgcaga taatacaggt gcaaagaaaa tcatggttat tgggtttata
    23221 ggtgtttcca agaaaagata tgcaagaact ggagatatag tcatttgtag tgttaaagat
    23281 gcacttccag atggtattgt taagaaaaaa gaaaaagtga aagcagtaat agttagaaca
    23341 gttaaaccaa taaaaaggaa agatggaaca attttaaaat ttgaccataa ttgtgctatt
    23401 atagtagata atgaaaaaaa tccaagagga acaagaattt ttggacctgt tccaagagaa
    23461 ttgagagata aaaattttac taaaattatt tctctagctc cggaggttgt ttaatgggaa
    23521 ttaaaataag aaaaaatgat tttgtttgtg taataaaagg taatgataga gggaaaaaag
    23581 ggagagtaat aaaagttttt ccagaagagg aaagagttct tgttgaaggt gtgaattttg
    23641 taaaaaaaca tacaagacca aggtccgttg atatgcaggg aggaataatt caaattgaaa
    23701 aaccaatatc aatttcaaat atccgatatt tctgcttaaa atgtggaaaa gcagtaggac
    23761 ttggtattaa gaaactatct gatggtacaa aagtgagata ttgtaaaaaa tgtaatgaga
    23821 ttgttgagga gaaatgatgg aaccgagatt aaaagaaaaa tataaagaag ttgtgattaa
    23881 ccatatgatg gaaaagttta attataaaaa taagatgcag gttccgcgat tggaaaaaat
    23941 agtaataaac attggaattg gaaaagataa taaagataca aaagcaattg aatcagcgga
    24001 aaaggaatta tctcttataa cagggcaaaa accagttata acaaaagcaa aaacttcaat
    24061 agcagcattt aatttaagaa agggagatat atgtggatta atggtaactt taaggggaaa
    24121 aagaatgtgg gaatttcttg atagaacgat tgttgctgca ctcccgagag ttagggactt
    24181 taatgggctg ccagtaaatt ctactgacgg aaggggcagt tatacaatcg ggataaaaga
    24241 acatgttatt tttcctgaaa ttgattataa cactatttat aaaattagag ggatgaatat
    24301 tacattggtt acaaatgcga aaacacctga agaaacactt gaattgttaa gggaaatcgg
    24361 attaccaata aggagggaat gaattggcaa gaaaagcatt aattgaaaag tcaaatagag
    24421 aaccaaaatt taaagtgagg ggcaaaaata ggtgttttag atgtggcagg gcaagaggat
    24481 attatagaaa atttggaatt tgtcgacttt gttttagaga gttagcatca aaaggagaaa
    24541 ttccaggggt aaaaaaagca agttggtgag gtgtatattc atccaatttt taggagaggt
    24601 ggaaaaatga cagttacgga cccaatagca gatatgataa caagaattag aaatggtaat
    24661 ttgaattata agagatcggt tgatattcct tttagtaaaa tgaaagaaaa aattgctatt
    24721 gtcttgaagg aagaaggatt tattaaaaat tatgagataa tagaaattga aaataaaaaa
    24781 gtaataagga tttatcttat gtattatcca gataggaaaa gagttattac tgagataaaa
    24841 agagtaagta agccgggtgc aagagtatac ataaagaaag aaaatacaaa taaagtaata
    24901 aaaaatggat ttggaatggc tgttttgaca acatcaaaag gaattattag tgataaaaaa
    24961 gcaagggaaa taggaattgg tggagaaatt ttatttacaa tatggtgagg ggtaaaaatg
    25021 gcaagattag gtaaaagacc tgttgaaata ccagagggag taaaaggaaa ggtagaaaat
    25081 aatagaatta taattgaagg tccaaaaggg aaattagaaa aggaagtatt gtcctatcta
    25141 aaagttgaaa ttattgataa caataaaata tttgtaaaaa atataattcc agaagagaat
    25201 aggaagttat ataaaaaggg tggggagttt cagggattaa tgagaagttt aattaaaaat
    25261 atggttgttg gagtcaaaga tggatatgag aaagttcttg aaattcatgg agttggttat
    25321 aaaggagagg taaaagaaaa aaaacttgtt ttaacacttg gattttctca tcctattgag
    25381 attgatatac catttgatgt ttcagttgaa gttgcaagaa atacagtaat ttttgtaaga
    25441 ggtatagata aacaaagagt ttcaagtttt gcagcaatag ttaaaaaaat cgctccacca
    25501 gaaccgtata aaggaaaagg aataagatat agaggagaat atgtaagaca taaagcaggt
    25561 aaggctgctg tgggaacagg acaatgaaaa taaaaaaaga agaaaaaaga aagagaaagc
    25621 atttgaaggt aagaagaaag gtgaaaggaa attgggaaag attacgactt gtggtcttca
    25681 gaagtcagaa gcatatttat gcttcttttg ttaatgatga tgaaacacca tgtaaggttc
    25741 tagcgactgt ttcaacatta tcggagggat ttaaaaaata ttcagaaggg aaaaatatta
    25801 agacatggac aaaggatgca gcaggaattg ttgggcagtt agtagcagaa aaagcaaaag
    25861 aaattggtat tgaaaaaatt acctttgata gaggtggata caaatatact ggaagattaa
    25921 aggttttagc agaatcagca agaaaaggag gtctaaaatt ttgaaagaaa atgaaataac
    25981 ccagcaggag aatgaaaaac aagcagttat tgaaataaga agagttacaa aagttacaaa
    26041 aggtggaaag aatttgaatt tcagagcagt tgttgttgtt ggagatggta aagggaaatg
    26101 tggatatggt attgggaaag cagttgaggt tcctgatgca ataaaaaagg gagttgataa
    26161 agcacgaaaa aatatgattg aaattccaat tgtgaacaca acaatacctc atgatataac
    26221 tgcaaaaagt tcatcttcta ttgttatatt aaaaccagca gttaaagggc atggtatggt
    26281 tgcaggtaga acaatgaggg caatatttga agttatagga ataaaagata taacaagcaa
    26341 atgtttaggg tctacaaatg cattaaatgt tctttcagca actataaagg cattgagtca
    26401 gttaaagaag aaaaaagagg ataaaaaaaa tgaaactgaa tgagttaaag gtaccttatg
    26461 gagcaaaaaa gaagagaaag cgggttggaa gaggggatgc ttctggacat ggagggactt
    26521 ctaccaaggg acataaggga cataaagcaa ggagtggata tacgcgttct tttaattttg
    26581 aaggtggaca aatgccattg gtaaggagat taccaaaaag aggttttaca catttaaaag
    26641 aaaaaatagt tgaaattgtt aaaattgaag aacttgaaaa taagttcaat gatggagaaa
    26701 atgttgatgt tgaattatta aaaaaagaaa aattagtaaa aaaaggtgaa tttgttaaaa
    26761 ttcttggtaa tggtaaatta acaaaaaaaa taaatgtttc tgccaatgct ttttcaaaaa
    26821 aagcaaaaga aaaaattgaa aatgttggtg gtaaaacaat tttactggaa aaaattaaaa
    26881 aatgatagat gcgtttaaag atacatttaa aattgctgat ttaagaagaa aaatagggat
    26941 tacaattttt ttaataagtg tatatcgttt aggttgttat attccctctc ccggtataaa
    27001 tggacaggct cttgcaagtt tttttgaaaa ttttccaaat actctttttc aattagttga
    27061 cctttttgca gggggagcat taagaaatgc aacaattttt gcgttaggaa taatgcctta
    27121 cataagtatt tctattatac ttgaacttct ttcctctata atcccatatt ttgaaaatct
    27181 tgtgagaggt ggagttgaag gaagaagaaa acttactcaa ttaacaagaa taggtacagt
    27241 aattttatgt ttatttcagg gttttgcaat aagtttatgg cttgaaaatc cagagcattt
    27301 tgctggatat gtaattgttc caaatcctgg atggactttt aaaattgtta caatgctaac
    27361 tttaacagca ggaactattt ttttaatgta tttgggagaa caaataacag aaaagggagt
    27421 tggtaatggt atatcattaa taataacagc cagcattctt tcagaaatgc ctattgcagt
    27481 acatagaatt gcacaacttg tttctgctgg tgaaataagt ttatttgttg ttcttctatt
    27541 gattgttttg atttttattg tagttgctgc tgctattatt gtaaatgaaa gtgaaaggag
    27601 aataccaatt caatatgcaa aaagagttgt tggcagaaag gtttatgggg gacaatcaac
    27661 ttatttacct ataaagttaa accagggagg agttattcct ttaatttttg caatctcaat
    27721 tttaactttt cctgcaacaa ttttaacttt ttctgaaaat aaagttatac aaaaagttgc
    27781 tgcatattta tcaccaagtt caggaccagg gatgtttctt tatattgttt tgataatttt
    27841 cttctgttat ttttatgcaa gtataatttt cagtccagac cgtgttgcag aggacttaca
    27901 aaaatatggc ggatatattc agggtataag acctggaaaa tcaactgctc tttatcttga
    27961 aagaattcta aatagaataa ctttacctgg tgctttgatg cttgcggcta ttgctatatt
    28021 tccatatata attatgagtg cttacaaaaa aattccatat atagttgcac atatttttgg
    28081 tggtattggt cttttaattg ttgtttctgt tttacttgaa acaatccgac agattgagtc
    28141 tcatcttctt ttaagacatt atcagggatt tattaaaaag gaaggaataa gatgaagaaa
    28201 atcttgatac ttttagggcc cccaggagca ggaaaaggga ctgttggaag aaatataagt
    28261 tctttttata atttgccttt aatttcttct ggtgacctat tgagagacaa tgtgaaaaac
    28321 catactcaac ttggtgaaaa ggcatatttt tatattaaaa aaggggaact tgttcctgac
    28381 gaaattgttg aggatattat taaagaaagg ataggacatg aagattgtaa aaatggattt
    28441 attcttgatg gttttccgag aactttaaaa caggcagagg atttaaataa aatacttatg
    28501 aataataata aagtaattgt tatttatctt tctgcctctg atcatttttt aattaatcgg
    28561 ctttctaata gaagaatttg tgaaaaatgt ggatatattt accatcttat aaatttacca
    28621 ccaaaaaaag atggtatttg tgataaatgt ggaggaagat taattcaaag agaagatgat
    28681 aaatcagaag tggtaaagaa tagactagat gtttatcata gttatactgg accaattatt
    28741 gactattaca agaaaaatgg caatttctat gaaatttctg gcgaagggga acttgaagat
    28801 acattaagcg aaataaaaag gattttaaaa tgatagttca aacatctgat gaaatagaaa
    28861 aagtaaaaaa agcatgtgta atagtaagag aaattttaga aggaataagc gaaattataa
    28921 agccaggaat aagtacatat gatattgatg aacttgccta tgagatgatg aaaaaaaggg
    28981 aagttgtacc tgcctttaag gactatggag gatttcctgc aaatatttgt gtttcaatta
    29041 atgaagaggt tgtgcatgga attcctaaaa aggagagaat attgaaagaa ggagatattg
    29101 tttcagttga tattggggta atttatgatg gattttatgg tgattgtgca gatacatttc
    29161 ctgttggaga gataaagcag atacataaaa aactaattga tgttactaaa aaagcatttt
    29221 atattggagt ggattacata aaagatggag taatgacagg tgatataggg aattcaattc
    29281 agcaatttgt tgaaagtaat gggttttcag ttgtaaaggc gttcgctgga cacggtattg
    29341 gtaaaaaatt acatgatttc cctgaattag caaattttgg gaataagggc gaaggagaaa
    29401 ttttgaaaga tggtatggtt atatgtattg agccaatggt taatgaaggt acatggaaag
    29461 tgaaaattct tgatgatgga tggactgcta taacttctga tagaaaatat tctgctcatt
    29521 atgaaaatga agttcttgta agaaaagaaa aaggagaaat tttaagttaa atgaataaag
    29581 aggaaaaaat aagattaaaa ggagaaataa taaatgcttt accgggaact acatttgagg
    29641 taaaattaga aaatggccat aaggtttttg ctcatcttgc aggaaagatg agaatgcatt
    29701 atataaaaat aataccgggt gatgaagttt tagttgaaat ttcaccttat gatttaacaa
    29761 aaggtagaat aataaaaaga tattaaagga gttaaggaaa tgaaagtaag agcatcagtg
    29821 aagaaaatat gccctaaatg taaaataata agaagaaaag gaattgtaag ggttatttgt
    29881 acgaatcctc aggatggacc taaacataaa cagaaacaag gatgaggtga aatatggcaa
    29941 gaatacttgg ggttgaaatc cctgataata agaaaattga aatttcttta acttatattt
    30001 atggaattgg taagcccaca gcaaaaaaaa ttttgtcaga tacaggtgtt tctcccgaaa
    30061 aaagagttaa agagctgact gatgaagaat taagcaaaat aacaaattat atccagagaa
    30121 attttaaagt tgaaggtgat ttaaaaaaag aaatttctga aaatattaga agattagtag
    30181 aaataaattc ttatagaggt ttcagacata aacgttcctt acccgtaagg gggcaaagaa
    30241 caagaactaa tgcgagaaca cgaaaaggtc caagaaaaac agttggaata gttagagata
    30301 aaacagcaag gaaagcaatg agacaggaaa aaggaaagga agaataatat ggctgcgaga
    30361 aaatatgtaa aaaagagaaa aaaaagggta tctattgaaa gaggaattgt acatataaag
    30421 gcaactttta ataatacaat agttactcta acggatcttg aagggaaagt tttgttatgg
    30481 gcaagtgcag ggagtattgg ttttaaagga acaagaaaag gaacaccttt tgctgctcaa
    30541 cttgtgagtg aaaatgttgc caaaagagca ataaacatgt ttaatatgaa agaagtatct
    30601 gtttttgtta aaggacctgg ccctggaagg gaaacagcaa taagggcatt acaatcaact
    30661 ggcttaaatg ttgtttcaat aaaagatgta accccgatac cacatgatgg ttgtcgacca
    30721 ccgaaaagaa ggagagtata atggcgaaag ttgttgaaac agattgtaaa gtttgtagaa
    30781 aatatggaat aaagttatat ttaaaaggca taaggtgtga atcggataaa tgtgcaatag
    30841 accccagaag aaagaatata agattaataa agagtaaaaa gacatcagaa tatgggcatc
    30901 aattaagaga aaaaaacaag gtaaagatat actatggagt aagagaaaga caatttagaa
    30961 gatattttga aatggcaaag aaacaaaagg gtgttactgg tgaaaattta ctccaacttc
    31021 ttgaaagacg tcttgataat gttgtttata gggcaaattt tgtctcttcg agaaaacaag
    31081 caaaacaaat tatttctcat ggtgcagtta taatcaatgg gagaaaagca gatagggatg
    31141 gatatattgt aaaagaaggg gaagaaataa aaattaaaac aaatattaaa tacatagaaa
    31201 aagttaaaga aaatattgat atttttaaac atagacagat tccaacatgg atgaaagtta
    31261 acaatgaaaa ttattcaata gaaattgtaa gatatccaac acgggaagaa gtttctatac
    31321 ctgtggatga gcaactcatt atcaaccttt attcaaaata aaaaatgtga ggtgtaaata
    31381 tgaaagaatt tttgtttccc agtagtttta tttgggatga agatacatac agagagaatt
    31441 atggaattat tgaaattcaa ccgcttgaaa gaggttatgg agtcacctta ggtaatttgt
    31501 acagaagaat tcttctttct tcaatccctg ggatatcaat aacatctctg aaaattgatg
    31561 gtgttatgca tgagttttca acaattgaag gggtaaaaga agatgtagtt gaaatagtta
    31621 tgaatttgaa aaatgttgct ttaaaaccag ttatatctga atttcctcat aaagtcgaaa
    31681 ttgaaataga gggaaaagat gaaatagtag caggggattt aatacccgat ggttccgcat
    31741 atgtaataaa tagtggtttg catattgcta cggttgaccc gactaagaaa tacaaaatgg
    31801 aaatagaaat aactaatggt tttggctatt tacctgttga aaaaatgaaa cttatgattt
    31861 ctactcctcc aattggtaca attttaatag atggaattta ttctcctatt aggaaagtat
    31921 cttttcatat tgaaaatata atggtaggcc aatcagttga ttatgaaaaa cttgttcttg
    31981 aaataggaac aactggtgca attactccga aagatgcagt tagttatgca acagacctta
    32041 ttcttagtca tctaaattta ataaaagata agcagacaat tggaaggaaa gaagtaaaaa
    32101 aagaagaagt tggcaagata gaccttgata ttccaataag tgacttaaaa ctttcaacaa
    32161 gagttattaa tgctttaaca ggtaaaaata ttaaaacaat tagggacctt ataaatactc
    32221 caagggaaaa attagaagaa ctaaaaaatt taggtaaaaa atccattaaa gaaatagaaa
    32281 atattttgaa aaaaagggac attaaactct taacattaaa agaattagca gaaaaacaag
    32341 aggaagaaga tgagacataa aaaagatatt aaaaaattaa gtagaaataa gtcccataga
    32401 aaagggctga taaggaacat gacaataagt tttttccaat caaatgagat aagaactaca
    32461 gaagttaaag gtaaacaatt aaagagagtt gttgaaagaa tgataacact tggtaaaaag
    32521 caggacttaa agagtacaag gatgctcaat gcttttttaa atcatccaga aagtgtaaaa
    32581 aaagttaaag aagttgcaaa gagatattct gatagaccag gtggatatac aaaaataatt
    32641 aaactttcca caagaagagg agatggagca agggaagtaa ttataaaatt agtgtgaaaa
    32701 atgaataaaa atttaaaact tcttgataaa gtatataaaa aagaaaatat atatcggctg
    32761 atagttattc ttgcaaaaag agcacatgaa ataatttcag gtgcacctgc aaaaataaaa
    32821 tcagacgaaa gtggcaatgc accacatgaa ttagtaatag aagaacattt acttagtggt
    32881 gataaaaaat aaaaaagtat aaaataaata ttcctgcagg ttgtgtacct tacctataag
    32941 gttcattaaa tattatttac aggataagaa aatgaatgat tttactcaat tactgaaatg
    33001 catgaaggaa aaagatgctt ctgaccttca tataaaattt ggaaggaaac caatttttag
    33061 aataaaagga gaattagaag aagtagaaga atgggagaat atttttaatg atgaagaatt
    33121 aaataaaatt tgttcaataa ttattccatc tgaacaaatt gagatattta agaaacaaaa
    33181 agaaatggac attgcctttg aacaggaaga agttggcagg tatagggtaa atgctttctg
    33241 gcaaagagga catattggtc ttgttttaag aagaataaaa gatgttatcc ccaccattga
    33301 agaattgaat ttaccacctg ttttaaataa aattgcttca ttttccgatg gacttgttct
    33361 tgttacaggt cctactggat gcggaaagtc aacaactctt gcttctatga ttgagaatat
    33421 taacaaaaat aaagttgttc atattgtaac aattgaagac ccaattgaat atatttataa
    33481 agataaaaag tcaattattg accagagaga ggttggaatt gatacaattg cttttgattc
    33541 tgctttgaaa cgtgttttaa gagaagaccc agatattatc ttaatcggag aattaagaga
    33601 tgtagataca tttcaggcag caataaatgc ctgtgagaca gggcatcttg tttttgctac
    33661 tttgcataca atagatacaa ttacaacaat tacaagaatt cttgattttt tcccatcttc
    33721 tcaacatgaa caagtaagaa aaattgttgc ctatcattta cgggcaacaa tatgtcaaaa
    33781 acttttaacc aaaaaagata attctggact tgttcctgtc tgtgaaatta tgattgtaaa
    33841 ttctgtaatt tctaaactta tacaggacaa caaattaaac aaaatatatg ctgctatggc
    33901 atcagataaa gaagtaggga tgcaaacatt taatcaacat cttgtccaat taattcaaaa
    33961 tgatataatt agtaaagaag aaggttttgc tgcatctcca tcaccacatg cacttgaaat
    34021 gaatttaaga ggaatttact tagatgagga ctcaaaaatt attggagaat aggaaagaac
    34081 cagacctttt tttctccatt tctatcattt caattatact aattttgtca ctcatattct
    34141 cttttttatt tagaaactta acaattgagg aaaagttatt taaggggggt atttttactt
    34201 ttttgactga agttggaatt atttactttt cttttatcaa atatccagaa tatttcaaaa
    34261 acatcttttc taatttttct cttttttttt ctggttttaa gtattatttg attataattc
    34321 ctattatttc aattgtctct ctcattgtat atgctatttt tgaacaatta catataaatg
    34381 tagaaattca aaaaatggtc tctttttatt tagaaattaa gtcaattaaa ataattttat
    34441 ttatgttttt tttaagttgt tttatcgctc ctttgacaga agaaataata tttagaggta
    34501 ttttatataa gtcattgaga aataattttt ctttttcaat tgctgtaatt ttaagttcat
    34561 tagttttttc tctttttcac aatgaccttt ttacttttgc cggaattttt atttttggtt
    34621 gtttcctgtc aattattttt gagaggcata ggtatctctg gccatcaata ggtcttcatt
    34681 ttttcaataa cttttttaca aatttatttg tattttttat taaatttcac caagcaggac
    34741 aaaattttgt gatataataa acgaaaaaag gcaaaaaatg aaaatacttg ttattggttc
    34801 tggtggaaga gaacattgta tttgctggaa aatcaaacaa aataaaaatg tacggttgtt
    34861 cgcattgcct ggaaatggag gaatatctga aattgctgaa tgtattaaag gtgatattga
    34921 agattttagt gatgccttta acattataaa aaataaagga attgattttg ttgtagtggg
    34981 acctgaaaat cctcttgttt atgggatagt agatttttta aaagaaaaaa acattaaggc
    35041 atttggtcca ataaaaaagg gtgctattct tgaaggttca aaatgttatg ccaaagagtt
    35101 tatgggaaag tataatattc caactgcaaa cttccagata gcagaaagtt atgagtcagc
    35161 aataaaaata attgaaaaaa aggaagtccc atatgttata aaatacgatg ggcttgctgc
    35221 tgggaaagga gttagagtaa ttgagagttt tgaagaaggt gaaaagtact taaaagaaat
    35281 ttttattgat aatattttta aaagtaatga aaagaaaatt ataattgaag attgtttaat
    35341 tgggaaagaa ctttcctatc ttatttttac agacacaatt tcatatattc caatggtgcc
    35401 agcaaaggat tacaaaagag tttttgatgg agataaagga caaaatacag gtggaatggg
    35461 ttcttattct ccaccacttt tttttaatga agaacttgat aataaaataa aaaatgaaat
    35521 agttgaaagg acaatatatg gattgaggaa agaaaatatt gattatagag gtgttttata
    35581 ttttggctta atgataactg aaaaaggtcc ttatgtttta gaatataatg ttaggtttgg
    35641 agacccggaa acacaggtga tattaccgag aatggaaaca gacattattg atgtaatgac
    35701 tgcggtttgc gaaggggaac tgaaaaaaat agatataaaa tggagtaata aaaaaagtgt
    35761 ttgtttaata ctatcttctg gtggatatcc aggaggatat gaaactggga aagaaataaa
    35821 aaatttagaa aaagttgaaa aaaatgtttt aatttttcat tcaggaacca aaaaagaaaa
    35881 tgggaagttt tatacaaatg gtggaagggt tcttggaata actgcactta actcaacaat
    35941 agaaaaagca agggaaaatg tttataaatc agcaagcacc attgattttg aagggaaaca
    36001 ttatagaaaa gatattgcac ttttttaata tataaaaatt aaaatgagaa aaaaggttat
    36061 tatatttggg gcagggcaga ttggaagggg ttttatagga gaaatatgtt atagttcagg
    36121 ttattttctt gtttttgttg atgtttttaa aaatgtaatt gacctactta ataaagaaaa
    36181 aaaatatcct ttatggatac tgggtaaaga aaaagaagag aaattcatag agaattttaa
    36241 aggtatatat tttgatgata aagaaagtgt atcaaatgaa ataaaagaag caaacttaat
    36301 ttttactgct gttggtgtga acaacttaaa atctcttgca cctttaattt ctgaagggat
    36361 tttgaaaaaa ttctctgcaa ataaatcgga ttatcttaat attataattt gtgagaattt
    36421 acttgggagt ggaaaaattt tatactcaga agttttagaa tatcttccac ctgaatttaa
    36481 agaagttgtt tttcaaaaag ttggttttgt tgagtcagtt gtaagcagaa tggtagcacc
    36541 tattactgat gaaataaaga aaataaatcc tttaattgtg agagttgaac catataacaa
    36601 attgcctgtt gataaaaagg catttaaagg agaaattcct gatattaaag gactttatcc
    36661 tgttgagaat ttatatccat atgaagaatt aaaaattttt gttcataatc tttcacatgc
    36721 gtgtcttgct tatttagggt atttaagtgg atatacatat atctggcaat gcatggaaga
    36781 taaaaaaatt ctaaaaattt ttaaaggcgt attaaatgag gttaaagaag cacttataaa
    36841 gaaacataaa tttgatgaga aagaaattga tgattatatt tatgaccttc aaacaagatt
    36901 taaaaataaa ttattagggg atactgtttt tcgcgttgga agagaccctt taaggaaact
    36961 tggtccagat gatagaataa ttggtagtat aaacttatgt ctttctcaaa atatctttcc
    37021 agccaatatt tgtgttgttg cttctgcctg tttatgttat aattaccaaa atgataagca
    37081 ggcagttgaa ttagaagaaa taataagaaa taatagtgtg gaatttgttc ttaaagaaat
    37141 atgtaaactg gaaaataaag aaattataga tgaaatttta aaacattatg aggaaataaa
    37201 aaatgaaagc agcacttcta aaagaaatag agaatttaga aattattgat attgaaaaac
    37261 caatacctgg agaaaatgaa atagttataa aagtaaaggt ttgtgctata tgtggaacag
    37321 atataaaggt ctatcatcat ggacataagt taattgtttt tccaagaatt acagggcatg
    37381 aagtaagtgg tattgttgct gaaaaaggta aaaaagtaag tgagtttaag gaaagtgata
    37441 aagttgctgt tgctcctgct attccgtgtg gaagatgctg gtattgtaga aagggacaac
    37501 aatcaatgtg tgataattta gaaacaattg gttatcttta taatggtgga tttgctgaat
    37561 atatgagagt tcctgaaaat gctgttttaa atggttgtgt aaataaatta cctgaaaatt
    37621 taagttttga agaagcagcg attgctgaac cacttgcttg tgtaataaat ggtcaaattt
    37681 taagtatgct tgaaatagga gatacagttg ttattatagg tgcaggacct attggatgtt
    37741 ttcacagtca acttgcaaaa ataaatggag caagtaaagt aatacttggt gatatttctt
    37801 ctgaaagatt agaaatggct aaatttacag gagcaaaact tgttgatatg agtaaagaaa
    37861 atataaaaga agttgtaaaa gaagaaacag aaggaaaaat ggcagacaga gttatagttg
    37921 cagcaggtgc aaaagaagca caagagacag cagttgaaat tgttgcaaaa agagggtcag
    37981 ttaatttttt tggagggctg ccaaaagaag taccttttat aaatttgaat agtaaccttg
    38041 ttcattatgg agaatttttt ataacaggta ctcacggttc ttctcctgtt cacaataaaa
    38101 tagcaatatc tcttcttgca aacaaaagaa tagatgggac gaaatacata actcataaat
    38161 ttccattaga agaaatcaaa caagcattaa aaacagcaga ggataaaaaa gggctgaaag
    38221 ttatagtaat tcctgaaaag tagagagtag caagtagcaa aattaaattt aaaattaaaa
    38281 gtgtaaagat agagcgtaaa attcaaaatt tttttgctgt cttgttttta attttactct
    38341 ttacatttta cttttttaat atcttcgtgt ctatgttttt agggcatggt tttatccgca
    38401 gattcgcccg gtggattatg tcaaggggga tgagggagta aattagttcc cccttttaaa
    38461 aaggcctgtc cgacgctctt tttggcggag ggattaaggg ggatttcacc cttctccccg
    38521 atggggagaa ggctcgcccg tagggctacg cccgagggcg aggatgaggg gatttttagg
    38581 aatttccaag cacagtggag aagtggggaa atttagttct ctctactctc tactcactac
    38641 taaaatataa acagagttta aaaaattttt aataacagaa gataaaggaa ggaggacttt
    38701 taaatggaaa agattgatag aaaacttgca ataaaacttt taaaacagat gatggaaata
    38761 aggaaatttg aagataaaat tatggaattg ctttcaaaaa acattgcaga aggtggttca
    38821 catctatacg caggaatgga agggacagga gttggagcaa tttctgtttt aagacctgat
    38881 gactatataa caagcacaca tagaggacat ggtcatgcaa ttgcaaaaga tggggactta
    38941 aaagcactta tggcagagat attagggaaa aaaacaggtg tttgtaaagg gaaaggtggg
    39001 tctcttcatt tagcagacct ttcaaaaggt aatttagggg caaatggaat tgttggaggt
    39061 gggcttggaa ttgcaacagg ggcaggtctt tcaataaaat tacaaaaaca ggataaagtt
    39121 gttatatgtt tttttggtga tggagcaact aataatggta tattttttga atgtttgaat
    39181 atggcatctt tatggaaatt accagttatt tatttatgtg agaataataa atatggaatg
    39241 agtgtatcag ttgaaagagc atcttctgtt gttgatttaa ctaaaaaggc acttgctttt
    39301 gatatgccaa gtgaaaatgt agatggacag gatgttcttg cagtaagaga aattgtaaat
    39361 aaatgggtga attacgccag agatggaaat ggtccgagtt ttattgtatc aaatacatac
    39421 agatattatg gacatagtag aagtgacccg agggtataca ggacaaaaga agaagaaaag
    39481 ttctggaaag aaagagaccc aattttaatt ttttctgata aaatgaaaga agttggtgtt
    39541 ttaacagaag aagaaataag agaaatagat gaactaacag ataaagaaat agaagaagca
    39601 actgaatatg caataaatag tccagaccca cagccagaag aactttatac tgacttatat
    39661 gtttaacagg agatgaaaaa tgaaagagat aacttataga caggcgttaa atgaggcatt
    39721 tgacgaagaa atgatgagag atgaaaaagt atttattttt ggtgaagatg ttgcaattta
    39781 tggtggtgct tatggtgtta cattgggttt attagaaaaa tatggggata ccagagtaat
    39841 tgatactcct atatctgaaa atgctattgt tggaactgca cttggtgctg ctttaacagg
    39901 gatgagacca gttgcagaaa ttatgtatat tgattttatt ggtctttgta tggaccagtt
    39961 aaataatcaa gtggcaaaaa tcagatatat gtttggtgga aaatgtaccg ttcctttgac
    40021 aataagaaca gaaggtggtg caggaagaac attgggagca catcattcac agtcattaga
    40081 atcatggtta attcatattc ctggtataaa agttgttatg ccttcaactc cttatgatgc
    40141 gaaaggtctt ttgaaatcat caataagaga tgataatcct gttgttttta ttgaacataa
    40201 aatgctttat aacacaaaag gacctgttcc tgaagaagaa tatactatac caattggagt
    40261 tgcagatgtg aaaaaagaag ggaaagatgt aactattttt acatattcaa ggatgacatt
    40321 attttcactt caagcagcag aagaacttga aaaagaagga ataagtgtag aagttgttga
    40381 tttaagaaca ctccttccaa tggatattga aacaatggta aattcagtaa aaaaaacaaa
    40441 taaagttgtt atagtggaag aagattgtaa aacgggggga acaggcgcag aaatagggat
    40501 gcaaataata gaaaatgctt ttgattattt agatgctcca attatcaggg ttgcaggtgc
    40561 agatgttcct atgccaaaaa gtaaagttct tgaacaactt gcaatacctg atgttaaaag
    40621 aattaaagaa ggagtgaagg aggtattgaa atgataagag aaattattat gccaaaatta
    40681 ggagaaacaa tggaggaggg gtaccttgtt tcctggaaaa aacaagaagg tgaaaaagtt
    40741 gaaaaaggcg aagttctctt tgaagttatg agtgataaaa caaattttga agtagaatcg
    40801 ccttattctg gatatttaag aaagatactt tttagttcat ccgatgaagc aattcctgta
    40861 acaacagtta taggttatat aagtgataat ccagatgaac aattacctga gaaagaagaa
    40921 aagattgaga aaaaagaagt aaaagaagag aaagtagaaa tagtacaaaa agaaaaagta
    40981 gaagaaaaaa tagtagaagg aggggagaaa ataaaagcaa cacctgttgc aaaaagaatt
    41041 gctcaggaaa gaggaataga tatttctaat ataaggggaa caggtcctgg tggaagaatt
    41101 gaaaaaaggg atgttgaaaa atatattgaa agtaaagcag ttggagtagg agaatatgaa
    41161 ataataaaat ggactcctat tagaaaaatt attgcaaaac gactgacaga aagtaaaaga
    41221 gaaattccac attattattt tcaaggaaaa tttattatgg accagattgc acttataaag
    41281 gaattgaaga aaaaagaagg taatgatttt tcatatactg attttctttt attttttgct
    41341 gggaaagcaa ttgagaaata ccctttaatt gatgcttctg ttgaaaatga tgagataaga
    41401 gtttataagt cagttgatat aggtcttgca gttgcagtag aaacggggct tgttgttcct
    41461 gtaataagaa atgtctgtca gaaaacactt gaagaaatat ctgaagaaag aaaggtaatt
    41521 gcaaagaaat caaaggaagg gaaattagaa gagaatgatt taaaaggagc aaggtttgtt
    41581 atttcaaatt tagggatgta tggagttgag aattttcagc caataataaa tccaccagga
    41641 gttgcaatta tgggagttgg taaaatagaa aaaggtatta ttgttataga agataaaatt
    41701 gaagtaaaaa gaattatgag tgtttctttt tcttttgacc atagagttat tgatggaagt
    41761 tatgctggct ctttttataa ctattttaaa gaaatacttg aaaatccctc aattttagtt
    41821 ttataatata tacaattagg cacaaagtaa aaaaagagag aaaatttata aatttaaagg
    41881 taaaagtgta aattttaaaa tgataactaa taatttaaaa ctgaaagcaa aagatgaaaa
    41941 ttatgacttg aaatctaaaa atttagttct tttttgtttt caattgtaca ttttacacta
    42001 ctaataactt tgtgctatat atgtatggtt tatattgtat atataaaact taatgacaaa
    42061 tgatagggag aataaaagag gatattaaca ctgtttttga aaaagaccca gcagcaagaa
    42121 atatttttga agtaattaca agttatcctg gtttacatgc gctgtgggga catcgttttg
    42181 ctcatttttt atggaaaaga aatatgaaaa catttgcaag gataatttct aatgtgaata
    42241 gattttttac acaaattgaa attcatccag gagcagaaat aggaagaaga ttttttattg
    42301 accatggttg tggagttgtt attggagaaa caacagaaat tggtaatgat gttttgcttt
    42361 accagggggt tgttttaggc ggaataactc atgagaagaa gaaaagacat ccaacaattg
    42421 gaaataatgt agttattgga gcaggagcaa ttgttttggg tccagttaaa gttggggatg
    42481 gtgcaaaaat tggtgcaggt agtgttgtgg ttaaagatgt tccatctgat tcaacagttg
    42541 ttggtgttcc aggtagagtt gtgaaacaac agaaaaaaag gaaaatagaa cttgaccata
    42601 gtaatcttcc cgaccctgtt gcagatgcat taaaattagt tattgatgaa attgaaaagc
    42661 atgaagaaag gattataaaa cttgaaagtg tagaaggtat taaagcaaaa atagatgagt
    42721 attttgctat gaaaaaagaa gaaatttctg aaattttttc aaaaataaag gagaatgaaa
    42781 atgaaaaaaa ttaatatttt atttttatcc tcattattta ttttcatttt atctttttca
    42841 ggatattgtg aaatagtaaa ggtctatcat aaagttaaaa gaggcgagac cttaattgca
    42901 attgctaaaa aatatggaac aagtgtagaa aagataaaag aactaaatgg tttaaaaaca
    42961 tctcttataa aagaaggaca aaaacttgtt gttaaaaaaa tagagaaaga ggaaaataaa
    43021 aatttatctt caaataaaga atcattaaca acctcttctg gaaattatga cacagtttat
    43081 tatgtagtaa aaaaaggtga taacctttct aaaataagta gaaaatatga catttcagtt
    43141 tcatctataa aaaaagaaaa taatttaaaa agcagtaaaa taataccagg aatgcagtta
    43201 aaaattagag ttccagttga agtgcctaaa atagaaaata tagagccaat aataggaact
    43261 gaacaaaagg atttttatca ggtaaaaaag ggagatactc ttgaagaaat agcaaagaaa
    43321 ttttctgttt tgcctgaaga acttaaaaaa tataatcttt tgcgtgataa tgattttaaa
    43381 gaagggcaga ttataattat tccccaacaa gagcaattaa caagtaatcc agaaagtcaa
    43441 acaaatagcg aaacagatat agaaaaagaa caattaacaa gagcaaaaat tcttgatatt
    43501 gcattttcct atcttaatat gccttacaaa ttaggtggga atggggaaaa atgtattgat
    43561 tgttcaactc ttactcgcct tgtttatgaa aaaataggtt ttgaattacc aaaaacatct
    43621 tatcaacaag caaaattggg caaagaagtt acattaaatg aagcattacc tggtgatttg
    43681 gtatttttta gaagaggcat aggaattgga catgttggta tttatatcgg taataaactt
    43741 tttattcacg ccagtagcag tgataggaaa gttaaaatag attctcttga aaattcgtat
    43801 tttaaaagtc attttgtatg tttaaaaagg tatattccat ttagtagcag cgtttttgga
    43861 ggttaaaaat gatgtcaaga aatagtaatt gtaggtgctg tgaatattta tggaatagtt
    43921 taaaaatagt tgtaattgaa aatgaaattt tgagaataga aattcttgca gacaaaggaa
    43981 cagatatagt tgaatttctt tacaaaccag aagatataga tttcatgtgg agaagtccta
    44041 ttcctttata taatcgtttt atgttcccta taacaaaaga gttagattca ggaaatttta
    44101 ttgattttta tccaggtgga tggcaagaaa tatttccaaa tggtagttcc ccatgtgaat
    44161 ataaaggaac ggttttggga ttacatggag aaattgctct actaccatgg gattataaaa
    44221 ttattgaaga ttcttccgaa aaaatttcag tccttttttc agtaaaaaca tatagaacac
    44281 cattttacat tgaaaaaatt atatctattg agaaagaaaa accatatatt gaaattgagg
    44341 aaaaagtaaa aaatattggt ggtgaagata tggattttat atggggacac catcctgcta
    44401 ttggtaaacc atttctttca tctgattgtt ttataaaaat tcctgaatgt aaaattaaag
    44461 ttgttcatgg tgatggaaaa tcaattacaa atttaaaaca aaccgaagga atatggccat
    44521 atttagaagg aattgataga aataaagttg atataagtaa aattccctct gaaaatgatt
    44581 gtgtatcaga tatgatgttt ttaactgacc ttaaagaagg aaaatatgaa attgttaata
    44641 aaaaaaggaa attatcattc ttttttgaat ttcctttatc agtatttaag tgtttatggt
    44701 tctggagaat tgcaaaaggc agttttaatt atccctggta tggaagaaat tataacattg
    44761 ctcttgaacc attttcttct ttaccaaatc tggcagaagc aataaaaaga ggagaccagt
    44821 tgaaattaaa accagatgaa gaaatgagag taaagataaa agcaggggta ataaaggaag
    44881 aaaatgggtg aatatgaagt tgtaattggg cttgaagttc atgtggaatt atctacttgc
    44941 tcaaaaatgt tttgtccttg ttcaacagaa tttggtgctt ctccaaatac gaatatctgc
    45001 cctatttgta taggaatgcc aggtacttta cctgtaataa atgagaaagc aattgaatgt
    45061 gcagtaaaaa caggtattgc atttaattct gatatagcaa ggacttgtag gtttgcaagg
    45121 aaaaattatt tttatccaga ccttcctaaa aattatcaga tttcacaata cgaacaacca
    45181 attataaagg ggggatacct gaaggtaaga gataaaaaaa taaatttaac aaggatacat
    45241 ttagaagaag atgcaggtaa acttattcat tctgaggagg gtgagaattt ttcatatatt
    45301 gattataaca gaggcggaat acctcttatt gaaattgtta cacaaccaga tataacaagt
    45361 ccagaagaag gtgaagaatt tctcttgaaa ttaaagcaga cattaaaata tttagaagta
    45421 agcgattgta atatggaaga aggttcatta agatgtgatg caaatatatc tgtaagaaaa
    45481 aagggagatg aaaagtttgg agtaaaagtt gagattaaaa atatgaattc ttttaaagcg
    45541 gtcagaaaag cgttaagtta tgaacaaaaa aggcaaattt caattttaaa tagaggtgga
    45601 aagattcagc aagaaacacg actctgggat gataaattac aaaaaacaga aatcatgagg
    45661 acgaaagaag aggcacatga ttacagatat tttcctgacc cagacctttt accagttgaa
    45721 attgatgacg aattcataaa taaagttaaa gaagaaatag gggaattacc agatgataga
    45781 aaagagagaa ttaaagtaca atatggatta cctacctatg atattgatgt cttgatgagt
    45841 gaaaaaataa taattgacta ttttgaggaa tgtagtaaaa ttttcaaaaa ttataaactt
    45901 atttccaatt ggattatggg agagataatg tctttattaa aagggaaaga aataaaattc
    45961 ctttttgaaa aaatacctcc agaaaaattt attgacttgt taaaaatggt tgaagagggt
    46021 aaaataacct caacagttgg gaaaaatgta ttaaaggaaa ttttaaaaac agggaaattt
    46081 ccttctgaaa tagtaaaaga aaaaggtctt ctacaaatag gagatgagga atttattgaa
    46141 aatatagtaa aacaggtaat tgacgaaaat caaaaagcag ttgatgacta taaaaaaggg
    46201 aaagaaaaag taatggcttt ccttataggg caggttatga agaaaacaaa gggaaaagtt
    46261 aattcagaaa ttgttgaaaa aacaataaaa aagttaatat aacaaaaaat aattttattt
    46321 ttccgtctat tttatatttt tctctttcct aataggaaga gatgaaggga gaaagaaaat
    46381 gagaaaaaag ttttttttaa ctattattgg gtttttctta ttaaccataa ttttaggtag
    46441 tgatgtaaaa gtttctgagg aaagaaataa aaaagaagaa acatataaaa acatagaact
    46501 ttttattgat gctttaaata ttgtagaaaa aaattatgta aaagaagttg aaaataagga
    46561 ccttatttat ggagcactta aaggtatgct gatgtctctt gacccataca gtgcttttct
    46621 tcctcctgac cttaaaaaag aattacaaat tgaaacagaa gggcagtttg aaggagttgg
    46681 aatggaaatt acactgaaaa atgggataat aacagtggta agcccaatag aagacagtcc
    46741 tgcctggaaa gcaggtgtta agccaggtga tagaataatt actattgatg gggaatcaac
    46801 aaaaggtatg acaacacttg aagcagcaaa aaaactgaga ggagaaaaag ggacaaaagt
    46861 gaaaataagt gttatgagag aaaatatgcc aaaactaatt gatatagaaa taacaagaga
    46921 tattataaaa gtgaaatcag taaaaacaga ggacataggt gaaatgggat atataagaat
    46981 tacagaattt caagaaagaa catcttctga tttgaaaaat gcattagaag agtttaataa
    47041 aaaagagaaa aaagggataa ttcttgattt aagaaacaac cctggtggtc ttttaacctc
    47101 tgctgttgaa gttagtgaat tatttttgca aaaaggtaaa ttaattgttt atacacaggg
    47161 aagaaataaa gaagataaaa gtatttttag aagtaaaaaa gttcctatct gggaaaaacc
    47221 aattgtttta cttgttaacc agggtagtgc cagtgcttct gaaattgttt ttggggcgct
    47281 taaagacaat tatgaaaaaa ttagctcggt tggtgaaaaa acattcggta aaggttctgt
    47341 tcagaattta attccgttat cagatggttc cgctttaaaa cttacaattg cttattacta
    47401 tacaccttct aatgtttgta ttgaaggaga agggatacca gttgattatg aagtgaaatt
    47461 accagaagat aaagaaatta tatttgcaac agatgaagat gtacaatttc aagaagcaaa
    47521 aagaattttg aagaatatga tttatgagaa agatgaaaaa gagtgataaa aaagagacat
    47581 catggcttgt acctattgtt ctccttttaa ttttaggtat tttaattaca ggtgtaaaag
    47641 ttcttaatga aatttacaaa aaagaggaaa ttccaggtaa aaaaatacct gccaaaaagg
    47701 aaaaagtagt tgaaaaaaaa gagaaaaaaa gagaaaaaga aattccagtt gcaataataa
    47761 tagatgattg tggctggaat aggtctattg taaacgaatt agaaggaatt aacgaaccac
    47821 ttacactttc ttttttgcca aaagctccat atagtaaaga attaattaaa ttgcttaatg
    47881 ggaaagatta tgaatttatc ttacatcttc cgcttgaacc attacctcct caacaatgtc
    47941 tggataaagg acttattaca acagaaatga ctgatgagga gataaataaa atatttcttg
    48001 ataatgtata tgattattta ccatatatca aaggaattaa caatcatatg ggttctcttt
    48061 ttacttctac tcctgataaa atggaagtac ttcttgaaaa tataaaaaaa gaaaatttgt
    48121 ttttcattga tagtgcaaca tcaaaaaata caactggtta tacacttgcc gagcagatgg
    48181 gaataaaaac agcaaaaaga gatatttttc ttgataatga atcaaatcca gaatatatta
    48241 aagagcagtt aaataaactt attaaagtag caaaaaaaaa tggaaaatgt gttgctattg
    48301 gacatgcaaa aaaagaaaca ttaaaggtat taaaagaaaa attacctgaa ctgaaagaag
    48361 aaggtataaa aatagttcct gcctctgcat tagttgaata aattcctcct cccggaacca
    48421 tgttccagaa aaaaggtgaa ggtctatgat aataattggt atagaaacat cctgtgatga
    48481 aacaagtatt ggtattttag aaaataataa attattagca aatattgttg cttcacagga
    48541 agatatacac tctaaatttg gtggtgttgt tcctgaactt gcaagcaggc aacacttaga
    48601 gaagatagat attcttttta atattgctgt tgaaaaaacc aaaattaata ttaacaatat
    48661 aaattatatt ggtgtcacat ctcaaccagg acttaaaggt tcattaattg ttggtgtttc
    48721 atttgcgtcg gggctttctt tttctttaaa aaaaccaatt tataaaattg accatttaaa
    48781 agcacatata tcagcaaatt attttgatga aaatattgag tttcctgcaa ttggacttgt
    48841 gatttccgga gggcatactt ctttttacta tcttaaaaac catattgatt ttgaactttt
    48901 aggaagaact cttgatgatg cctgtggaga agtatttgac aaagttggca gaatgttagc
    48961 ccttcccttt cctggtggac cacaaataga aaaaaacgca aaagaaggag atgaaaataa
    49021 aataaaattt ccagttgcct taatttcaaa aaactccctt gatttcagtt ttagtggatt
    49081 gaaaacagca gttaattatc acattaaaaa aaatggatta aataatattc ctgatatatg
    49141 tgcttcattt caaaaagttg ttgcagatac attatgtata aaaattgaaa gagcaataaa
    49201 tagatataaa gtaaaatccc ttcttttggg tggaggagta gttcaaaatg aatatataag
    49261 gggaagaata aaagaaaaaa ttggagaaaa aattaaattg tttatccctg aaaaaaaatt
    49321 atgtatggat aatggtggta tggtggcgat tttaacatat tttttaatta aatatggagt
    49381 aaaaccatca gaaaataaaa tagaagtaat tccaacagta gaaatttgat tttttataaa
    49441 atgtcaaaag gaaaaataga ataaaacctc tgcatattac aattggt
//
LOCUS       DAOOVW010000008        45483 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_513_length_45483_cov_8.617571, whole genome shotgun sequence.
ACCESSION   DAOOVW010000008 DAOOVW010000000
VERSION     DAOOVW010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 45483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45483
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_513_length_45483_cov_8.617571"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..1766)
                     /gene="rny"
                     /locus_tag="PLW95_02045"
     CDS             complement(192..1766)
                     /gene="rny"
                     /locus_tag="PLW95_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOV21448.1"
                     /translation="MSTINTLKLVIPLFILWGAIAVLIGYLWRKFIAERTIKTAEKRA
                     KEIINEAENLALSKKKEIDIEAKETLYRLRNEFEKETRNKKKELQFQEKRLQQREMNL
                     EKKAELIEKHSEEIEKTQEEINKKQEEIKQKELSLLSLIEEEKRKLETIAGIPREEAK
                     NQLLKLMEEEAKKEAIKLQQKLEEEAKINSEKKAREILLGAMQRLAPEEASESVVSVV
                     SLPNDEIKGRIIGREGRNIRAFESLTGVDLIVDDTPEAVTLSSFNLYRRELARVALER
                     LISDGRIHPGKIEEVIEKVKQDLSEQMVEEGNNTVFEVGIENVHPELIKILGKLKYRT
                     SFGQNVLIHSKESAFLAGMIASELKFDPKIAKRAALFHDIGKAVDQEVEGAHPEIGAE
                     ILQKYGEKEEVIEAALYHHKDINIAKPYTVITQVADAISATRPGARRDTLEKYLRRIE
                     DLEKIASSFKGVDTAYAISAGREIRVIVKPEIITDEEAKNLAREIAKSIEENLEFIGQ
                     VKVVVIREKREIDFAK"
     gene            complement(1926..3500)
                     /gene="serA"
                     /locus_tag="PLW95_02050"
     CDS             complement(1926..3500)
                     /gene="serA"
                     /locus_tag="PLW95_02050"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOV21449.1"
                     /translation="MDKIKVLVTDPLKEAGIKVLEKAGFEVTQVGKKKVEELLDIIEK
                     YDVVVVRSETKITKDVIERGKNLKIIGRAGVGLDNIDVEAATAHGIIVMNAPEGNTIS
                     AAEHAIALMLSLVRNIPAANYHVKSGKWERKKFMGTELHNKTLGIIGLGRIGKRVSSI
                     GKGFGMKIIGYDPFVEGIALREMGVTIISLDGLLKQADFISFHIPLTDQTYHMIGEKE
                     FEMMKEGVYIINCARGGIIDEDALYKYIKKGKVKGAGLDVFEKEPPENSPLLELEQVV
                     VTPHLGASTEEAQENVAVQLAEQIVEAITKKVVRNSANVPYLDEETMKKLRGYLSLGE
                     KMGSFLSQMVESEIKNIVINYYGEIVHYDLTLVRYYIIMGLFKNYGVNVVNAPLILKE
                     KKINLRESRQETGGEFVNLITVEVSNGKSISVSGTILENKEERIVKIDGFSVDASPKG
                     YLLVCYNEDTPGIMGHIGTVLGKEGVNIASMTLGRKKKGGPAITVLNLDQSIDKKVLN
                     KIKEFPAINSVKLVII"
     gene            3701..4966
                     /locus_tag="PLW95_02055"
     CDS             3701..4966
                     /locus_tag="PLW95_02055"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOV21450.1"
                     /translation="MFYFLIYDFVFFIVLTLFFPFIWKKIRTEKDYPGTWKERFAIYR
                     NNLKRGKRKRVWLHTVSVGEFLSITPLIEKLKDIDIDIIVSLTTKTGRKVAISKTPEL
                     KYIFFPFDFYPIMFKAISFLKPDIIVIVETEIWVNLIRISKRKNIPVVMINGRLSPKS
                     FRRYKKLKFLTKRILPHFEKIAMRSESEKRKIISLGAKKEKIRICGSMKFDLAYQMGQ
                     TINSDEFKKSLNLGIDKKIIVFGSIHPEEEEEICKISKEIIENYPEWDVIIAPRFLDR
                     SKIFNVLTEMKLPYVKRTEIRENFRILVVDTYGELNNFYSICEFAFVGGSLNRWGGQN
                     PIEPIAFKKVVIYGKDNWHFFIEWEKIKKGGGGIEVNDFKQLKEKVLFLIKNENIRKK
                     MGEVAYKILLQNTGATDINFDIIKETLGI"
     gene            5054..5905
                     /locus_tag="PLW95_02060"
     CDS             5054..5905
                     /locus_tag="PLW95_02060"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007954328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HOV21451.1"
                     /translation="MEFQKVQRKIEDFWLKKGCVLWHPYNVEVGAGTLNPATFFGILG
                     NKKWSVVYLEPSVRPADGRYGENPVRLYMHHQIQVIIKPPPANIQDIYIKSLEYIGLK
                     RNEHDIRFIEDNWEAPTLGAWGVGWEVWIDGLEITQFTYMQQVGGIDLFPPAIEITYG
                     LDRIVKFIQKKENIYDIVWAGGIKFGDIQKAREKDYSIYSFEIADTEFIFKLFDMIEK
                     EIEKVLEKNVLYPAYDYVLKLSHIFNILDARKAISVSERVRYIQRVRKYARKCAQLYI
                     ERGEGII"
     gene            5956..6037
                     /locus_tag="PLW95_02065"
     tRNA            5956..6037
                     /locus_tag="PLW95_02065"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5990..5992,aa:Leu,seq:gag)
     gene            6549..6731
                     /locus_tag="PLW95_02070"
     CDS             6549..6731
                     /locus_tag="PLW95_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21452.1"
                     /translation="MAKTPLWEDILIIISIFSVWPAILRKENIFTKVIMFFAFFLLIW
                     VFIRRLKRFNNMNNEK"
     gene            6721..7242
                     /locus_tag="PLW95_02075"
     CDS             6721..7242
                     /locus_tag="PLW95_02075"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21453.1"
                     /translation="MKNKLFFLFFLLIFLAGCSYEKETFRKLEKQGDTLFSQGRKKEA
                     IERWQDALKFKKTSCIYEKMVVTLIMERENEQATMWAEQGLTYFPDCCNLIYNLSYLC
                     LLEKNYDKCLKNIERLLAINEYYPEAHFMKGMIFEQTGRIKEAKEEYVKEININPGCK
                     KAWKKIKEIGDEK"
     gene            7232..>10881
                     /locus_tag="PLW95_02080"
     CDS             7232..>10881
                     /locus_tag="PLW95_02080"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOV21454.1"
                     /translation="MKSKRCFLFLLFAVFLFLYSISYSETLTDYLKFFSSFNSRIPGY
                     EGNKKSAEYIEEKFKEIGLVNVKKEEFFTSIPIEKYSYIKVGNEEIKIYSLWPNLVRT
                     TTLPPEGLEGKLFYAGKGDLKNLDGKDIEGGIGVLDFDSDDSWTTLAGFGVKGFIFLS
                     SPDIERKEAEKKFLRIPVNVPRFYAGENSDKIKALAIKEEKVKLFSKVDWENVPNYNI
                     YGTIEGNDPKLKDEIILLESYYDSISVVPSIAPGATSACGISTLLKLAEFFANSHPER
                     TLVFLATSSHFEYLKGIDNFIQNHSRKEEPFFSRIPKKEKILPALSICLELSDGSDEL
                     AIWHNSYDFYKQRYFAPFAKKFLSYSEKVCTKLGVDPQTALMNGVSPEKGIIWQTFLP
                     EAIRTDGEYLILSGNPAISFVTANDGRWRVDTPVDTFENLNLENIEKQFIFLQNILDI
                     ALNDKDFFPDTELELKDTMSSLIPKIVTFNPKKSFVPNDPVNEGLVVPRMIYYNPGLY
                     SMKTFIGVRGDIIEMTNKDGEVNISRFSKFSPPLEVYIYAFFIDKNTGEITLTTDLGV
                     NGDQQYPIKTLIDYKEKKKTTVLFKCKPISLFGLIDPQYLVSLDKLDVFDLSNSTPDA
                     YGYYLEYPLFIPFIWSSDSEPAGCIFAKFNSKIKVIGESGPLGKRLLLLNSKQTLNNK
                     EDAEGLGFDVDSIEAIYNIPYQGAKDMIILDTYRRVNFEKYGIRNERLKDLQIASNEF
                     LEQATKAKKEKKWYDFLKFSRQAQAIESRAYPDVKGTANDVVKGIIFYFILLLPFAYF
                     AERLFFSFSKLEQRIAGISGIFLVVYWIMRLVHPAFKLTNAPEVILLSFIVLALSVVV
                     LSVITSKFEEQMQRMKRETSNVYETDVGRITATTTAFSLGVANMKRRKVRTMLTAITL
                     ILLTFTVLSFTSIKSYLKFNQLLRPNTPPYTGILLRDRSWAPLQKFALQYTEDEFSSN
                     SIISPRGWYVLSQLGNKTAVEVKCGDEIFLSSGILGLSPQESEISPIKDTIISGKWIE
                     GNSENNVLISKKIANALKLTNEDIGKTLNIYGKQFTLIGIFDGKKLGQIKDMDDEPIT
                     PVDFSSLPEKEVNKMKMEKSAQVYTSQAKLQTFTHVEGENTIILPYKTLLKMGGTLHS
                     IAVKFSPEITSPATTVENFITKLAIILFAGYENKTFVYSSMGMTSFAGLTNLIIPILI
                     AALIVLNTMLGSV"
     assembly_gap    10882..10981
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10982..11799
                     /locus_tag="PLW95_02085"
     CDS             <10982..11799
                     /locus_tag="PLW95_02085"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOV21455.1"
                     /translation="IPILIAALIVLNTMLGSVYERLREIGTYSAIGLAPVHIASLFLA
                     ESLVYAVLGAVAGYLLGQILSKILMATDLLKGLVLNYSSMSAVFSTIIIFITVILSTI
                     YPARKASQMAVPDVTRKWILPEPRGDKWEFEFPFTVSQYEVLGLVTFLTEYFNSFQDV
                     SLGNFYTNGAKLIYEKHQSGKKKYILETEIYLSPFDLGVGQNFKMVLEPLGQYDFYTI
                     NLEMERKSGESNDWKRLNRRFLDNIRKQFLIWRTVSSEIKKEYTKKGKELLNL"
     gene            11985..13967
                     /locus_tag="PLW95_02090"
     CDS             11985..13967
                     /locus_tag="PLW95_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide transporter"
                     /protein_id="HOV21456.1"
                     /translation="MENKIKDDSYQVEIEEPFQEGFNKKTVLAALFIGFLMLPGSIYL
                     GLITGVGLGGAAQWVTVILLVEIAKRSFVQLKKQEVLIIYLIAHSLVAAGITIGTASL
                     VLQGGAFSDLIWKQYLVQSPYAESFGLTPHIPSWAVPPAGSEVLLKRTFLSRVWLIPI
                     LLIIIHNVLFRINYFSLGYILFRITSDREKLAFPMAPVASEGAIAMAEVSAKKDTWRW
                     KVFSISAMIGVIYGAFYTVIPTITGLLMTKPFQIIPIPWLDFTEKIGLIFPAAILGFV
                     TDLGSIFTGFVLPFWVVSGTFIGSIVASVLLNPILYKRGIIKNWQPGMTAVPANVVSQ
                     MDFWVNIMIAGAIVVGIIGVVRGLGNFIKTKNTVTTEKKLPAGRGDYPIPIAFGIWFI
                     ATLIYIIICHILVPKFPVILFIFFGFILTPFLSYTSARMFGITGVAGGSDFPMVREAT
                     FILSGYKGADIWFAPVPYFVIGGQVQTFKQLELTRTKFTSWYKGEFTALGIMLFCSFL
                     FWSIIWRMNPIPSSTYPFVQKMWPMSAIFQSLWATSTLETGQNWMIKAIKLKYISGGA
                     GIYFLLYFLFTIFKFPIFMFYGIIGGVAMMPHYVLPMFFGALLGKFYFSKKIGKETWR
                     KYTPIILAGYACGMGLIGMISVAVALIAKTIFQIIF"
     gene            complement(13964..15358)
                     /locus_tag="PLW95_02095"
     CDS             complement(13964..15358)
                     /locus_tag="PLW95_02095"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOV21457.1"
                     /translation="MKLKILGAGKNVTGSKFLIETKEKRFLIDCGLFQEREFKNRNWQ
                     DLPVSPDTIDFIILTHAHLDHCGYLPKIVKDGFKGEIYCTYPTEEITRIVLLDSAKLQ
                     EEDAEKKRIRHQEERRITPFPEIPLYTIEDAENVFSLFRPKKYREEIIVSDNISFTFH
                     DAGHILGSSMVELVIKEGREIKKIIFSGDIGRWNKVILEDPTFFSSADIVFMESTYGN
                     RIHESENQGGEKLGDIINETVQKGGNVVIPTFAIERAQELLYFLKKLLIEDKIPHIFV
                     FVDSPMAISITEVFEKYTEYFDEETRKMFKQGNSPFDFPLLKFTRSVNESKAINHIKS
                     SSIIMAGSGMCTGGRIKHHLITNITREECTILFVGYQAKGTLGREILEKKENIRILGQ
                     FYPLKARVESINGFSAHADRNELFFWIDKIKNVKNIFLIHGEEEVISEFSSFLKEKLS
                     SSIYVPDYLEELNI"
     gene            complement(15389..15463)
                     /locus_tag="PLW95_02100"
     tRNA            complement(15389..15463)
                     /locus_tag="PLW95_02100"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15426..15428),aa:Arg,seq:cct)
     gene            complement(15664..15873)
                     /locus_tag="PLW95_02105"
     CDS             complement(15664..15873)
                     /locus_tag="PLW95_02105"
                     /inference="COORDINATES: protein motif:HMM:NF021560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HOV21458.1"
                     /translation="MKITYDSKYDVLYLKFKDIKEVVCKNLTDDITIDMDERGKIAGI
                     EILSASKHIDLDKLLPIETKEKISV"
     gene            complement(15870..16145)
                     /locus_tag="PLW95_02110"
     CDS             complement(15870..16145)
                     /locus_tag="PLW95_02110"
                     /inference="COORDINATES: protein motif:HMM:NF025442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HOV21459.1"
                     /translation="MGKRIIIIEHARDRIKNRGANISEVEETIKKGQRYEVRYSIQAK
                     EMVSPYGKKWQDKFYSQKKIRVIYKEEKNQLIVITVYVYFGKWEDRK"
     gene            16687..17970
                     /locus_tag="PLW95_02115"
     CDS             16687..17970
                     /locus_tag="PLW95_02115"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOV21460.1"
                     /translation="MGITIRRELYVKAQDKNMCEWRSQKYVNGEGLKRVEIRSLEGES
                     DWSSGLFERYSDDNGRTWSQWKDIYSQGYEKKGEDEISTNYGCETYNPQYKHFVSLMM
                     NRIFLGGHTQAYKRFWEKGEAGFIDHCFLSVRNDKSQNSSVKLIKYEEGADYDPDNWL
                     NPEYITRNRAYFGCNIDILNNGEIIFPIGANVKACCRILSLNINDIFPSCPDIMCGMI
                     VVRGIFNQSSGNYDFIFSKPVVISDLKSSRGIDEPAAISLPSGRIIAVFRGSNVECKD
                     WNTRIEPGTPSHKWYCWSDDGGKTFTDPVPWHFDNREVFYSPASISNFIKSISNGKIY
                     WIGNITGYNAYGGHPRYPLVIAEVNERGLLKKETLTIIDTRQEGESKEVQLSNFSILQ
                     DRETGLIELYLTKIGQHEGYTWWADCYRYFIDVNK"
     gene            18309..19511
                     /gene="tyrS"
                     /locus_tag="PLW95_02120"
     CDS             18309..19511
                     /gene="tyrS"
                     /locus_tag="PLW95_02120"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOV21461.1"
                     /translation="MNKIEEKFEIIKKNTVEIINEDELKKKIRKSIEEKRPLRVKYGI
                     DPTAPEIHLGHTVPIKKLRDFQRLGDKILFLIGDFTARIGDPTGRKETRPILSVEEIK
                     KNLLRYRQQVAKLLDIEKVEFVYNSTWLAKLKLEEIIELTSIFTVAQILERDDFTKRY
                     KEGYPIYLHEFFYPLLQGYDSYHLKADIEIGATEQKFNLLAGRTIQEFFGQSKQVVIT
                     MPILVGIDGKLKMSKSYGNHIPIETTPDEMFGKIMSIPDDIMKDYFLLLTGIPSEIFS
                     KKIEENPRQAKCFLAKEIVREFFGEEQARKAEDKFNKIFRDKETPEEIPEFKIPADLL
                     EDSKVNVIDLIYASGIISSKSEIKRLISQGGIKINGEKVKDTDFVFEVKDGQIIKIGK
                     RKFLKIKR"
     gene            19514..20167
                     /locus_tag="PLW95_02125"
     CDS             19514..20167
                     /locus_tag="PLW95_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOV21462.1"
                     /translation="MENIREKLTEYGKKIVEKKLVAGSGGNISAREGNFVYLSPSGFF
                     LDDIKNNEWVKVDIKTGEIYGELKPTCEISTHLGIYKEREDVKVVFHTHPPLTVGLIS
                     SGMDFKPFFPDFVAVLGKEVPVIDYVPPGGEEMRKRVVEKIKNSNVVLIRNHGAICIG
                     ETFKEAFARAWLVEDTAKSLIASLISGKLRYFTKKEIKGIENLESEDYRKAILKKME"
     gene            20167..21012
                     /gene="folP"
                     /locus_tag="PLW95_02130"
     CDS             20167..21012
                     /gene="folP"
                     /locus_tag="PLW95_02130"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOV21463.1"
                     /translation="MKEKWKVDKKNILLKNVLIAGILNITSDSFYDGGKFIDIEKAIV
                     RGKEIIKDGADIIDIGGESSRPGSIPISEKEEIKRVIPVIKELSSENIIISVDTYKSK
                     VAEEAIKNGAKIINDISALEMDRKLVNILKTSECGYVLMHMKGTPKIMQENPYYKDTI
                     GEITEFFKVKLEYIEKNGIDIERVVIDPGIGFGKRVIDNILILKHLDKFKKFKRPIFI
                     GTSRKAFIGKILNLEVEQRLEGSLATVVYAYLKGGRIFRVHDVKETKRVLDIIQAIEN
                     ENIGT"
     gene            20993..21436
                     /gene="ruvX"
                     /locus_tag="PLW95_02135"
     CDS             20993..21436
                     /gene="ruvX"
                     /locus_tag="PLW95_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOV21464.1"
                     /translation="MRILGLDIGTKRIGVAISDESCTIARGLGILERNGKEINKIKEI
                     IEKYNIEKIIYGLPLRMDGSISGQTELVISFISKLKNEIKIPLKEWDERLSTKNAETI
                     LIQADISRKKRKKLIDKLSAQIILQNYLDSKTLENKTIEDIEHEY"
     gene            21597..22613
                     /locus_tag="PLW95_02140"
     CDS             21597..22613
                     /locus_tag="PLW95_02140"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOV21465.1"
                     /translation="MRKIKCAVVGTGIFGELHVETYASYDKADLLIICDSNEDRVKKV
                     SKKYNVKYTLDYKEVAENEEIEAVSVATPDFAHRDIVVEMLQKGKHVLVEKPMATNVK
                     DAEIMLEAAKESGKILMIDFHNRFNPAFIQTKERFDNGDFGDIGMIYTRMSDAISVPL
                     KWFSWSDKSGPQWFLFPHIVDLVTWISGEKVKKVYGIGKKEVLKSHGLDIYDTVTGIL
                     DFGKFYATVETSWIIPEVWTSICDFYFKLEGSKGRVEADFGDMGLKIASDVKKTMEIP
                     LYTGYIPTYGKLQGFQPLPIMYFIDCILENKKPFITPEEGLENVKIISSIIKSVETGK
                     VVEI"
     gene            22625..23401
                     /locus_tag="PLW95_02145"
     CDS             22625..23401
                     /locus_tag="PLW95_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOV21466.1"
                     /translation="MKLGIVTYQIAADWDIDTIIEKCKKLGYEGVELRTGHKHGVETT
                     LSKQEREDIKKKFQESNIKLVGLGSVFEYHSPDKEELRKNIEGTKEYIILAKDVGAEG
                     VKVRPNAFPENVPREKTIEQIGISLREVSSFAADYGIKIRLEVHGKETSYPYYIKQMI
                     NIANHQNLYICWNSNITDIDETGSIEKNFNLLKDKIDICHINELCNEYPWIELFKLLN
                     RINFDGFCLAEIQASSEPDRLLSYYKVLFNTYNKIVQTEK"
     gene            complement(23406..25199)
                     /locus_tag="PLW95_02150"
     CDS             complement(23406..25199)
                     /locus_tag="PLW95_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV21467.1"
                     /translation="MIKEFFKIKNYVKVRWHLLVAGIFFTILSVFANGVSLSAIVPLM
                     DKVLVKKEKIILPENLPQIIKIKIEPLIFKINQLPSLLLLKYLLIFLITILFLKGLFF
                     YLQNYFYRCFSTRLLTDVRELLYTKIMSLSDDFFAKKQTGELTTRIIYDVNLLNVVFE
                     SFFPKFVFPILLTFSYFVIIFTIDWKMSLLSIFMFPVILYPVFKMGNKLRKLGKKIQI
                     AYGEIGNVINESVYGQQIIKVYNREEYMKEKFKKENENIFKNVISMVKRLLIISPFTE
                     FAGAVASSGLIYYGAVKIINGGMSTGFLFLFFVALLSMISPLKGIGETYGLIKQASSA
                     LPRVFSVLDTESSVVEEGKDVFMGLKKNIEFKDVFFSFEGKQVLKGISFIVEKSEKIG
                     IVGPTGVGKTTLVGLLLRFYNPEKGEILIDGKNIKEYKINSLRQHIGFVSQQPVLFHS
                     TVKENITLGFENEERFKEVIKMDGIAQLINSLPEGENTIVGERGITLSGGQKQLISIA
                     RAIYKDPDILIFDEATSSLDTESEKMIQKAIDQTLENRTSFIIAHRLSTLKKVNKIIV
                     LKDGTIVEEGTHQQLLDKQGVYYTLWQLQFA"
     gene            complement(25196..26293)
                     /gene="lpxB"
                     /locus_tag="PLW95_02155"
     CDS             complement(25196..26293)
                     /gene="lpxB"
                     /locus_tag="PLW95_02155"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOV21468.1"
                     /translation="MKIGIFTGEVSGDNYAAILAKNIKEINPDIEIFGTGGKILEEVG
                     VEIIKGMPVGYMGYSSVILKLPSYLKFFKKIIYEIEKRIPDIIVFIDNPAFNLKVAEK
                     LKRKFFNVYYIPPKIWAHNYKRIKKIKKYINFVIPIFPFEENIYKKEGIKCTYFGHPV
                     VDLIKKGEDRKRDKKMIGILPGSRIQEVSYTLPVILDIVYDLKKEIDFDVGISSFDEE
                     IEMVIKKICKRKRIEFEIFKGFPYTLIENSDLILSTSGTVNLEVSLLLKPLIVFYKTS
                     FLNYFIGRCMVKLNMVSPVNLFIGENVVPEYIQKIPKEKVKKDIIDLIGKKEIYQKEI
                     KSFKYLKEKIGEEGVSKKVAEFILELTKGKK"
     gene            complement(26295..26822)
                     /locus_tag="PLW95_02160"
     CDS             complement(26295..26822)
                     /locus_tag="PLW95_02160"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HOV21469.1"
                     /translation="MKKRILIFGFFVFVVNVYAFDIKKPSSIDYFVGYGEGKLERQPD
                     YQFPIFAVDFSYPLSDVWDFQLEPFVSYVYKPENNFEVGLAFFFKYNFFRNKRISPYI
                     KGGSGIIYISQDTYEQSTNFNFVDQICGGIKFSFKKNIIFLEYRYRHISNAGIDHPNS
                     GINSNLYLFGISHPF"
     gene            complement(26940..28592)
                     /locus_tag="PLW95_02165"
     CDS             complement(26940..28592)
                     /locus_tag="PLW95_02165"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HOV21470.1"
                     /translation="MARYKIGRKIRRYREIANIVIKYGFGYLLEKAHIKLSILPIRKK
                     NQILSPPIRLRKMLEELGPTFIKMGQILSTRPDLVPIEYLKELEKLQDSTEPVEYEKI
                     KDVLEKNLKCKIEDTFDYFDEKSIASASISQVHIASYKKEKVAVKIQKPEIEEKIFLD
                     MEILYDIAQLIERFVKEASIYRPVKIVEEFEKSVRKELDFSYEGRNIQRFRKNFSSYE
                     NIIIPDVYKELTGTKVLTLQFIEGVKITEIDKINSIDRKKLAQQGTEIILKQIFQDGF
                     FHADPHPGNILITKEGKFCLLDFGIVGRLTEEEKLQIISLLSGAIKANSEKIIKTLEA
                     MGSYQAEEIDKRKLEREIDDYIEKYRDISLGEINIDVLFDEIFSIMRKYKISVPANFS
                     LLAKAILTLEGVATIIYPEYNISEYLQTYVLDFIEKKEKVKGILKDTLRNISSTYDIL
                     KELPETVEASLKMLKKGYINVAFEHKGLQNLTSTIDKSSSRVAFSLIISAILVSSSLI
                     MVSGKGPKLWGHSAFGVVGFIVSGIFGIYLIISIIRNSRIIK"
     gene            complement(28827..30290)
                     /locus_tag="PLW95_02170"
     CDS             complement(28827..30290)
                     /locus_tag="PLW95_02170"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HOV21471.1"
                     /translation="MLTLEERVKNIMEKGGSRKAVAGIINMGLKTPVTRRALINAIEK
                     ATYKMAFKGPDRPRKVMEERYYMGSALLSSLQKAFDSGSISKNCVKALSNIFLGKIFI
                     EGIYVRRTFEEKHGFRPPCFVTISPTNVCNLKCKGCYAGEIYTKDTLKFDVFDKVISE
                     IKEQFGAYFFVISGGEPFMYRENGKTLFDILEKHNDAYFMAYTNGTMIKKDVAKKLSE
                     LGNFTPAISVEGFEKETDERRGNGIYKKIMEAMDNLKENGVPFGISVTPTRYNAELLL
                     SDEFIDYYFNKKGAFYGWYFQYMPIGRKPSVELMVTPEQRYKMLKRIWKLVIEDKIFI
                     ADFWNSGTASDGCMAAARGGGYFYIMWDGTITPCVFIPFKDKNYGNLYQLYNEGRTIT
                     DAIKSPLFEEIRKWQNNYWIERPACQCGNLLTPCIIRDNSTDFYEIVKKTGAVPLDEG
                     AKEYLSFIEQGKMPQYNKEYRELVEPLWKNEYLGKSE"
     gene            30478..31242
                     /locus_tag="PLW95_02175"
     CDS             30478..31242
                     /locus_tag="PLW95_02175"
                     /inference="COORDINATES: protein motif:HMM:NF013161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOV21472.1"
                     /translation="MEIKNCKVIKNQKIAEKHFILKFENKFSKEFLPGQFLMVALPHF
                     YLRRPFSVFKVNKNTISILYKVVGKGTEILSKMKKGNILNILGPCGNEFNIEQKYENI
                     WIIGGGTGIAPLIFLSEKLKRKNKNITFFYGARNKNLLFFDILPAGIEYVFSTNDGSY
                     GYKGTIFSIVEKMIKKEEKPDVIYCGGPELLLQKVSSLSVRHKIPSFITLENFMGCGM
                     GVCYGCVIKVKEQNGWEYKRVCKDGAIFRGEEVIWQ"
     gene            31233..32150
                     /locus_tag="PLW95_02180"
     CDS             31233..32150
                     /locus_tag="PLW95_02180"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOV21473.1"
                     /translation="MAVNLNTELGKLKLNSPIIMASGTFGYGTEKFQFIDYKKIGAIT
                     TKTITYEKWEGNPQPRISEIPSGMINNIGLQNPGIKYFCEKIYPSLEKLKKEEVKIFV
                     SITDRTLDNIIKIVEKLNYLKIDAIELNFSCPNFEKNNLMISQIPDKTFSVVKETKKV
                     SKFPVITKLSPNVTDIVEIAIAASEGGSDILCMVNTVKGIFFDWGRKKIIEGGISGQC
                     IKGIGLRCVYDVYKRVKIPIIGLGGIVCGKDALEYILIGADAFGIGSGFFSNPGIVND
                     VFNYLSSYLTKKGIKNIKQIVGILNEKGK"
     gene            32134..33921
                     /locus_tag="PLW95_02185"
     CDS             32134..33921
                     /locus_tag="PLW95_02185"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOV21474.1"
                     /translation="MKKENKKEKNYFLSILTILLCLFIFLRVWYDGISEPFINTLLVF
                     YIFFLTLISYLYRKEKTENLPLMYLPFFLFVLFSFISSGISQIKGSGITYNTNILSFF
                     CLMILISKIEKQQVKYLIYTLFFACFVLGIYGIYQYYWGFEITRQYILSNKEVLANLP
                     PTFLARLESNRIFSRFVYPNVYASFLLTVLPLSFFTTFEKNFYIKFFSWATFFIAFYN
                     LLLTGSIGGILIFIFVFHLIFLFLILPERKFFKITPFLILFEIIFVIFIYKAGKLPHI
                     HSFVDRLNYWKSSINIFKESPILGVGPENFAFFFTKYKLPQSMEAKHAHSLFFETLVE
                     NGIIGTIFLFSFVFSVLYNIFIKKEKQKDFFLYGIGFSFLSLLIHNIIDFDFVDPSSS
                     FLFFILAGISIVLLNGNYRKVKLTLFPFYFTIILVCCSITFTRYNLCIGNLEASKNID
                     NYNLKLYFLERAEKFYPFYNVYLYKGKVFCQMGTENRDEELLKKGENLLKYSIYLNPY
                     LVNAYRELSFLYQQEGRIKESEKMYLKLIELYPNKKQFNLEVSIFYKSLKNEEKFKYF
                     FEKSKYLFPVSVEEGIICNNYEKWIELQK"
     gene            33900..34565
                     /gene="ricT"
                     /locus_tag="PLW95_02190"
     CDS             33900..34565
                     /gene="ricT"
                     /locus_tag="PLW95_02190"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOV21475.1"
                     /translation="MDRIAEVEIRKCDKLLYFKVSKDVQVEKGDFVIVEFTQGTIDWG
                     RIYSFVETKGFFPHTISGKVLRKITDSDRIIIEENNAKKKDVFDIFNKKIKQYHLNMK
                     LVDVEYSFDRTRITFYYWADGRIDFRNLVKDLAKIFNCRIEMKQIGLRDEAKIKGGYG
                     ICGQPLCCYAFLKKFDTITMRFVKTQKLPLDMHKITGICGRLLCCLRYEDEFYKKEKV
                     GKK"
     gene            34562..36049
                     /gene="metG"
                     /locus_tag="PLW95_02195"
     CDS             34562..36049
                     /gene="metG"
                     /locus_tag="PLW95_02195"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOV21476.1"
                     /translation="MKFYITTPLYYVNAKPHIGHTYTTVAADCLARFYRMSGYDVFFL
                     TGTDEHGQKISIAAQNEGFLPEELADKISQIYKKTWEKLNISYTKFIRTTEKQHQEVV
                     KNVFNKLYEKGDIYKGQYQGYYCIHCESYVIPEEKENPVCPDCKRNVEKISETSYFFK
                     LSAYSEKLLRVIEESEFIKPDFRKKEITNFIKEGLYDLCLTRKNVKWGIPCPIDENYT
                     IYVWFDALLNYLSGVGYLYDNNTFEKYWPVDVQLIGKDIMRFHNIIWPAILMSLQLPL
                     PKTIFAHGWWTMSKEKISKSKGNVISPDDFINEYGVDPFRYFIMREIPFGLDGEYSAI
                     TFKKRYNSDLVNDFGNLLNRTLNLIEKNFNGIIPDSSSEEEITNLTDEVFENYSKKMK
                     EISFSEGIEEIWKLINYLNKYLDIKAPWRQTCEKPEKVIYSVVEGIKNVSLMLSPIIP
                     ETIKRVFQMIGIENKINKENFSSIKERIPCGIKTGKREVLYIRKK"
     gene            36046..36762
                     /gene="pyrH"
                     /locus_tag="PLW95_02200"
     CDS             36046..36762
                     /gene="pyrH"
                     /locus_tag="PLW95_02200"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOV21477.1"
                     /translation="MKPIYKRILVKLSGEALLGKKKSGISSDALVSISDEIKEVKNMG
                     CEIGVVVGGGNIYRGSQRDKEVIEPVTADYMGMLATVINGLALQNCLENKEGLETRVL
                     SAFEMREFCEPYIRRRAIRHLEKGRIVIFVGGTGNPYFTTDTSAALKAIEIKADVILK
                     ATKVDGVYTSDPLKEPSAKKFDKLTYMDILQNQLKIMDSTAISLCMENHIPIVVFNLF
                     KKGNLKRVVCGQGKRTIITD"
     gene            36988..37545
                     /gene="frr"
                     /locus_tag="PLW95_02205"
     CDS             36988..37545
                     /gene="frr"
                     /locus_tag="PLW95_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010093383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOV21478.1"
                     /translation="MKNQIVKEVDSKMKEVVNFFTKEIGLLRVGRASVSLLEGIIVEY
                     YGSKMPINQLSTITIPQPQLLIIQPWDKNIVGDMVKAIQSSNIGLNPISDGNVIRVPV
                     PPLSEESRKEIVKNLYKMAEEAKVEIREIRRQANIQLKEKQKNGEISEDDMYNGFDEI
                     QKMTDKYIEEIDKKTKDKEKEIMEL"
     gene            37542..38216
                     /gene="cmk"
                     /locus_tag="PLW95_02210"
     CDS             37542..38216
                     /gene="cmk"
                     /locus_tag="PLW95_02210"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOV21479.1"
                     /translation="MKNIVIAIDGPAGAGKSTVAKLVARKLGFLYIDTGAMYRCLTLK
                     AMENNINWNDKEKLIEMAKNTNIEFISNDNYKVIMDGRDVSEAIRDEEVSKNTKHIAS
                     ILEIREILWEKQRNYREKFNIVMEGRDIGSKVFPDAQIKIYLDASIDERAKRRYLQLK
                     QMGIEEEIEKIKKDVEQRDEVDRKREIAPLMKVPEAIYIDTTGLTIQQVVDTICDLVQ
                     PLYLGR"
     gene            complement(38199..39908)
                     /locus_tag="PLW95_02215"
     CDS             complement(38199..39908)
                     /locus_tag="PLW95_02215"
                     /inference="COORDINATES: protein motif:HMM:NF019496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21480.1"
                     /translation="MGKAIKVGEVKNSCQTPVIGVFAPSDPRIDEQSRQRVKNIIKMT
                     ADIIADRIKFFGEDVKVVYSDVLIDGEKQGDIVAKQFKEEKVDIFIGVPDTWAFPQLT
                     LISFLQQFPQDTPINLTCGNSGPKPGVVYVQACNGAVAQYGKLMHINIGNWSDTGLEP
                     IISDKTIQDLVDWCYGAITYKALKGRRVVIFGHDSMGMETALAHIIPTRKIFGIEITR
                     LDMKLLSDMLSKKVYDKNELTELRNWLEKYSGGIEIRNQEDSEKFNQSLAMYLIVRDI
                     MKDLNAVGGGFMSQLEWGSDLRGIPLPVADVMEALFNSTFDHTGKKRVIPYATEADVQ
                     GLLTMLFFTYLTGGEPPLFMDFRKVWEPWEIKQLAEKIGINIKTDEMWFKKGFVDGTN
                     SGSASFNWAGKQGEKMEEIMKRITFPIAEEFSFPGGGNSVKFVSPGGIKGIAGRLAYS
                     SINNIFSLIWDEAETGQLPEKLAEEVCKTSNYTWPHTFVVPKYASMLEYKQYAPANHF
                     HMIWDLKVSRLEYWMDFTNVLSVCPWQARPEFIEGVDRPLPLLYLINGGENNTKLKLS
                     SQV"
     gene            complement(40136..41455)
                     /locus_tag="PLW95_02220"
     CDS             complement(40136..41455)
                     /locus_tag="PLW95_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21481.1"
                     /translation="MSREKIKVGAAKYSIMPPLDQARRIGPKGLATDVIGELCARATV
                     FDDGENKVAIVLLDISEFFPNITRGIRELASKWTDIPQENIMVCATHTHSSAKVIDYD
                     DPYDTGGVLIRYDKLSNETQAYLDMLYRYAASSVFLADSRRRTAKGRIGEISVPGIGT
                     PRIRMKDGSVASLTHAYSIEHVKEEEIESISPYDDSLRLVLFEDTEGRPICGLGNFGC
                     HNALSMGSTTLNSDYFGWAAEKLEKEIGNKFVFSLMAGPEGNVHPAAFFEHDVPAAEA
                     EKFVPVAGQKLYEKAKEIWEKLKPFEPTPVISASKKVYFPLQKTLTLRHQAYLQGRRV
                     AGGKQDEKGVFSELQIICLGELVILGLCGEVFHEIAMNLRKASPFKYTWVNSLCNDEL
                     SYLMPSHEHKRDNESGIMNVQKEFALPDENAEEIIYKTYQELFNRVK"
     gene            complement(41526..42815)
                     /locus_tag="PLW95_02225"
     CDS             complement(41526..42815)
                     /locus_tag="PLW95_02225"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOV21482.1"
                     /translation="MKIAIFGGEKTVKEIKDWPPIDEIDRKYVLNSLEGTNHAYGPNC
                     VELEKEFAAWNGNKYAITTNSGTAALHMSIAGCGLGVGDEIIVPSYTWSSSATCILHH
                     NCIPVFVDIDYETMNMDVDKIEEKITEKTKGIIVVHLHGLSVEMDKVMEIAKKYNLKV
                     IEDACQSHGAKYKGKKVGTIGDCGAFSFNQNKCLCSGEGGMFVTDNEEIYEKAKMLWS
                     FGETRRPSESRDYHAYALGWMYRNNDLTAAFGRAQLTKLDGYLETQKKNASVLTDIIK
                     DIKGLKIPYTPEGCEHIWYNYTLRIEPEKLDFSGDEVILRDSIMEAIRQEGVPVGIWQ
                     SFILPEMTVFQAKNAYGKGCPWICPHSRNVSYNPEEYQEAQKHCRRHFGMTYPLRQPN
                     NEKTAELVGLAIKKVFDDIKEKGVEKWVSMVQEVKKS"
     gene            complement(42944..44350)
                     /locus_tag="PLW95_02230"
     CDS             complement(42944..44350)
                     /locus_tag="PLW95_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21483.1"
                     /translation="MEREKILIGWAKEDITPDRPVLLHGQHYARVSKYVNDPVTATSL
                     AIESEKGEQAILLSADLVYIYEEVCNEVREYVKGKMSEIDVQKISFGATHTHTGPTMK
                     EGLYETSEKGVMSPSEYTKFFVKKAGDCIIKSWQNRKEGMISYGLGHAVVGHNRRATY
                     FDGSSKMYGNTDDLNFSHIEGYEDHSVNLLFTWDKNKKLTGMIINIACPSQETENALF
                     LSADFWQDTREEIYKRYSKDIFILPQCSPAGDQSPHLLIGKKEEGRMLELRGFKEKEE
                     DIRMSARKEIGRRIANAVDDALPYTKEDIKDKVEFKHIVKTINLPMRMVTEEEVKQAK
                     QEIEKLQREKETPNTFRHLSRNKEVIERYNKQKENPYLPMELHVIRLGDIAFATNRFE
                     LFLDYGIRIKARSKSIQTFLIQLSNGGGTYLPTERAVKGRSYGAEIVDNLVGPEGGQV
                     IVEETLKEINNLFGSVKK"
     gene            complement(44360..45166)
                     /locus_tag="PLW95_02235"
     CDS             complement(44360..45166)
                     /locus_tag="PLW95_02235"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOV21484.1"
                     /translation="MKYSMMSYTMAGGKWGENPDIEYLCKLTRNLGLDGIEWVGLYGR
                     RADEVKEIMDDYGLKTVCYTFFVDINFKDENLRRHGIEQIKRGIEESKVLATDKIMLP
                     IGGKDEYSRDESRENVIKGLEEVIGLADNYGISITVEQFPDIRGPFLNSEDMKKIIKE
                     IPQVKVTFDSGNVILGGEEPSESFLKMKEYVIHTHFKDWEISNDGKEGLDGKKYIPAL
                     IGEGIIDYKKLVKTMEKENYLSYVSIEYEGYKYDREEAIIKALNFLKNIK"
     gene            complement(45241..45372)
                     /locus_tag="PLW95_02240"
     CDS             complement(45241..45372)
                     /locus_tag="PLW95_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21485.1"
                     /translation="MYERKILLVSYLLFPLWDILSNKDYTSPGEFYYTFYVEYVKII"
BASE COUNT        15648 a         6869 c         6846 g        16020 t
ORIGIN      
        1 aatagaaaaa gaagagaaaa ataaaagatg tgttggagta gatttttatt tgacataaca
       61 ggggttattt gttattgatt tgtaaaactt gttatttgtc ggtgtaaatg gaatggagat
      121 gagaaagaat tattgtaagg atatcttagg tagcccgatt catattgaga atcgggctac
      181 ctataaataa attatttagc aaaatcaatt tccctttttt ctcttattac aacaacttta
      241 acctgtccta taaactctaa attttcttct attgattttg ctatttctct tgctaaattt
      301 tttgcctctt catcagttat aatttctggc tttactatta cccttatttc tctccctgct
      361 gaaatagcat aagcagtatc aactcctttg aaagaagaag caattttttc aaggtcttca
      421 attcttctta aatatttttc aagtgtatct cttctggctc ctggtcttgt tgcagaaatt
      481 gcatctgcga cctgagtaat tactgtgtag ggttttgcta tatttatatc tttatgatga
      541 tataatgctg cctcaataac ttcttctttt tcaccatatt tttgtaaaat ttctgctcct
      601 atttcaggat gtgcaccttc aacttcttgg tcaactgcct ttcctatgtc gtggaaaagg
      661 gctgctcttt ttgcaatttt ggggtcaaat ttaagttctg atgcaatcat ccctgcaaga
      721 aaagcactct cttttgaatg aataagaaca ttttgcccaa aacttgttct atacttcaat
      781 ttaccaagta tcttaattaa ttctggatgc acattttcaa ttccaacttc aaatacagta
      841 ttattgccct cttcaaccat ttgttctgaa aggtcttgtt taactttttc aattacttct
      901 tcaatttttc caggatgaat tctaccatca gaaattaatc tttcaagagc aactcttgcc
      961 agttctctcc gatagagatt aaaagaagaa agagtaactg cttctggtgt atcatcaaca
     1021 ataaggtcta ctcctgttaa tgattcaaaa gcccttatgt ttcttccttc ccttcctata
     1081 attcttcctt taatttcatc gttaggcaaa ctaacaacag aaacaacact ttcacttgct
     1141 tcctcgggag caagtcgttg cattgcacca agaagtattt ctcttgcttt tttttctgaa
     1201 tttatttttg cttcttcttc taatttttgc tggagtttaa ttgcttcttt ctttgcttct
     1261 tcttccataa gtttcaaaag ttgatttttt gcctcttctc ttggaattcc tgctatagtt
     1321 tctaattttc ttttttcttc ttcaatcaaa gataacagag acaattcttt ttgttttatt
     1381 tcttcctgtt ttttatttat ctcttcctgt gttttttcta tttcctcact atgtttttca
     1441 attaactccg cttttttttc taaattcatt tcccgctgtt gtagacgctt ttcttgaaat
     1501 tgcagttctt tcttcttatt ccttgtttct ttttcaaact catttctcaa tctgtataat
     1561 gtttcttttg cctcaatatc aatttctttc ttcttactta atgcaaggtt ctctgcttca
     1621 ttaatgattt cttttgctct tttttctgct gtttttattg ttctttcagc aataaatttt
     1681 ctccacaaat aaccaattaa aactgcaatt gctccccata aaataaataa agggataact
     1741 aattttaaag tatttatagt ggacaattta aaccacctcc tttactttcg ctgttttaca
     1801 gcaaattaag taaaggaatc atccggcagg acaaaatgaa tgaggaatta gtgaactaat
     1861 ttttaaattt atctttatta atctatataa tttttcactt tttttctttt ttattttcat
     1921 tttcgtcata taattaccaa ttttactgaa tttattgccg gaaattcctt tatcttattt
     1981 aatacctttt tatctatact ctggtcaaga tttaaaactg ttattgctgg accacctttc
     2041 ttttttctac caagtgtcat tgatgcaata ttaactcctt ccttgccaag aactgttcca
     2101 atatgtccca ttattccagg agtatcttca ttataacaaa caagtaaata tccttttggt
     2161 gatgcatcta ctgaaaaacc atcaattttt actattcttt cttctttatt ttctaatatt
     2221 gtcccagaaa cagatatact tttcccattg cttacttcaa ctgttataag atttacaaat
     2281 tcgcctcctg tttcctgcct tgattctctt aaatttattt tcttctcctt taaaataagc
     2341 ggagcattaa caacattaac tccataattt ttgaaaagtc ccattataat gtaatatcta
     2401 acaagagtta ggtcataatg aacaatttct ccataataat ttattactat attttttatt
     2461 tctgactcta ccatttgaga gagaaaagag cccatttttt cccctaatga aagatacccc
     2521 cttaattttt tcatcgtttc ttcatcaaga taaggaacat ttgctgaatt tcttacaact
     2581 ttttttgtaa ttgcctcaac aatttgttct gctaattgga ctgcaacatt ttcctgtgct
     2641 tcttctgttg aagcgcctaa atgaggagta acaactacct gttctaattc aagtaaaggt
     2701 gaattttcag gtggctcttt ttcaaaaaca tctaaaccag cacctttcac tttccctttt
     2761 tttatatatt tataaagtgc atcttcatca attattcctc ctctggcaca atttataata
     2821 taaacacctt ctttcatcat ctcaaattct ttttctccaa tcatatgata tgtctggtct
     2881 gttaaaggta tatgaaaaga aataaaatct gcctgtttta ataaaccatc taaactaata
     2941 atagttaccc ccatttctct taaagctatt ccttctacaa aagggtcata gccaattatt
     3001 ttcattccaa atcccttccc aatagaagat actcttttcc caattcttcc aagcccgatt
     3061 attccaagtg ttttattatg aagttctgtt cccataaatt tttttctttc ccattttcca
     3121 ctttttacat gataattagc agcaggtata ttcctgacaa gtgatagcat aagagcaatt
     3181 gcatgttcag cagcagaaat ggtatttcct tcaggggcat tcataacaat aattccatga
     3241 gcagtggctg cttcaacatc aatattatcc aatccaactc ctgctcttcc aattatttta
     3301 aggtttttcc ctctttcaat tacatccttt gttatttttg tttcacttct tacaacgact
     3361 acatcatatt tttctataat atcaagaagt tcttctacct tttttttccc aacctgagta
     3421 acttcaaaac ccgctttttc aagaactttt attcctgcct ctttaagcgg gtcagttaca
     3481 agcactttaa tcttatccat ttttctctcc ttcattttat agaagcaaaa ttatcacttt
     3541 tgtcctagtt tgataaatta tactaccctc taaaaaaatg tcaaattagt gaatttagta
     3601 tatcaaattg gtatcattta ttttgttact cccacttctc aaattcttta caagatgttt
     3661 tttttattgt aaattatcac atcactacaa aaggttaaaa atgttttatt ttctaattta
     3721 tgattttgtt ttttttattg ttcttacctt atttttccca tttatctgga aaaaaataag
     3781 aacagaaaag gactaccctg gtacatggaa agaaaggttt gcaatataca ggaataactt
     3841 aaaaagggga aaaagaaaaa gggtctggct tcatactgtt tctgttggag aatttttatc
     3901 aattacaccc ctaatagaaa aattaaaaga cattgatatt gatataattg tttctttaac
     3961 aacgaagaca ggaagaaaag ttgctataag taaaacccct gaattaaaat atattttttt
     4021 tccttttgat ttttatccca ttatgtttaa agcaatatcc tttttgaaac cagatattat
     4081 cgtaattgtt gaaacagaaa tatgggtgaa tttaatcaga atcagtaaga gaaaaaacat
     4141 ccctgttgtt atgataaatg ggcgtctttc tccaaaatca tttagaagat ataaaaagtt
     4201 aaaatttctg acaaaaagaa tacttcctca ttttgagaaa attgcgatga gaagtgaatc
     4261 agaaaaaagg aaaataattt ccttaggtgc aaaaaaagaa aaaattagaa tatgtggaag
     4321 tatgaaattt gaccttgctt accagatggg acaaacaata aatagtgatg aatttaaaaa
     4381 atccctcaat cttggtatag ataaaaaaat tattgttttt ggttcaatac atccagaaga
     4441 agaggaagaa atatgtaaaa tatcaaaaga aataattgaa aattatcctg agtgggatgt
     4501 aataattgcc ccaagatttc ttgacagaag taaaattttt aatgttttaa cagagatgaa
     4561 attaccgtat gttaaaagaa cagaaataag agaaaatttt agaattcttg ttgttgatac
     4621 gtatggggaa cttaataatt tctattcaat ttgtgaattt gcttttgttg gaggaagttt
     4681 aaatagatgg ggaggacaga atccaattga gccaattgca tttaaaaaag tggttatata
     4741 tggaaaagat aattggcact tttttattga atgggaaaaa attaaaaaag gtggtggagg
     4801 aattgaagta aatgacttca agcagttaaa agaaaaggtg ttatttttaa taaagaacga
     4861 aaatataaga aaaaaaatgg gagaagttgc ttataaaatt ctacttcaaa atacaggagc
     4921 aactgatata aactttgata ttataaagga aacattaggg atatagatat aaaataataa
     4981 aaagttttta taaaacatta taatccctat tgtgaatttg acagtgggtg attgtcctga
     5041 taaaatcaaa aatatggaat ttcaaaaagt tcaaagaaaa attgaagatt tttggctcaa
     5101 aaagggatgt gtactatggc atccttataa tgttgaagtt ggagcaggga ctttaaaccc
     5161 tgcaactttt tttggtattc ttggaaataa aaaatggagt gttgtttatt tagaaccatc
     5221 tgtgagacct gctgatggaa gatatggaga aaatcctgtt agattatata tgcaccatca
     5281 gatacaggta ataataaaac cacctcctgc caatatccag gatatttata ttaaaagttt
     5341 agaatatata ggacttaaaa gaaatgaaca cgatataagg tttattgaag ataactggga
     5401 agcaccaaca cttggagcat ggggagttgg atgggaggtt tggattgatg ggcttgaaat
     5461 tacacaattt acctatatgc agcaagtagg aggaattgat ttatttcctc ctgctattga
     5521 gataacttat gggcttgata gaattgtaaa gtttattcaa aaaaaagaaa atatttatga
     5581 tattgtatgg gcaggtggca taaaatttgg cgatatacag aaagcaagag aaaaagatta
     5641 ttcaatatat agttttgaaa ttgcagatac tgaatttata tttaaattat ttgatatgat
     5701 tgaaaaagag attgaaaagg ttcttgaaaa aaatgtttta tatcccgctt atgattatgt
     5761 tttaaaatta tctcatattt tcaatatcct tgatgccaga aaagcaataa gtgttagtga
     5821 aagagttaga tatattcaga gagtgagaaa atatgcaagg aaatgtgctc aactttacat
     5881 agaaagaggg gaaggtataa tataaactga ataatcagaa ctttcttctt gtagagaagt
     5941 atggttaggt atagagccgg gatggtggaa ttggcagaca cgtacgtttg aggggcgtat
     6001 ggcttatgcc gtgggggttc aaatccccct cccggcacag agaaacaaga aagaagatat
     6061 ttgaaaacaa aagaaaagga attaaaacca cacggcataa aaagggtatg ggaaaaaact
     6121 ttttcccata tttatagtaa tacatttcgc attgggggta tcgttgggaa aggggaatca
     6181 cccctgtccc aggagcgaaa gcgacgccgt ggtgggttga ccccctcccg gcattgcaat
     6241 catctttgat aggggattta ataaaatccc gtaagcataa cttatcgctt cggcgtaggg
     6301 atggtctgta agcaaaactc taaacccttc tgtgtaagac aatccatccc ggccttcctt
     6361 tgataaagga aggagtagtt tccccctttg gaaaaggggg ttaaggggga ttttaattta
     6421 tatatccgcc aaaaaacgca ggatactata aaacgttttt tgtaccttgg gattaaaata
     6481 taaacttaat aaatatttac aatatttata aaaaaagtat aataaaaaac ttaggagaaa
     6541 aaagaaaatt ggcaaaaaca cctttatggg aagatatttt aataataatt tctatttttt
     6601 ctgtttggcc tgctatttta agaaaagaaa atatttttac aaaggttata atgttttttg
     6661 ctttcttttt acttatctgg gtttttataa ggcgtctcaa aagatttaat aatatgaata
     6721 atgaaaaata aattattttt tttattcttt ttgcttattt ttttagcagg atgttcttat
     6781 gaaaaagaaa catttagaaa attggaaaag cagggagata cacttttctc acagggtaga
     6841 aaaaaagaag caattgaaag atggcaggat gccttaaaat tcaaaaaaac tagttgtatt
     6901 tatgaaaaaa tggttgttac acttataatg gaaagagaaa atgagcaagc aacaatgtgg
     6961 gctgaacagg gtttgacata ttttcctgat tgttgtaatc ttatatataa tttatcttat
     7021 ttatgcctat tagaaaaaaa ttatgataaa tgtcttaaaa atatagagag gctactggca
     7081 ataaatgagt attatcccga agcacatttt atgaaaggca tgatttttga gcaaacaggc
     7141 aggataaaag aagcaaaaga agaatatgta aaagagatta atataaatcc tggctgtaaa
     7201 aaagcatgga aaaaaataaa ggagattgga gatgaaaagt aaaaggtgtt ttttgttttt
     7261 attgtttgct gtttttttat ttttatattc aatttcctat tcagaaacat tgactgatta
     7321 tttaaagttt ttctcttctt tcaattcaag aattcctggt tatgagggta ataaaaagtc
     7381 agcagaatat attgaggaaa aatttaaaga gattggattg gtaaatgtca aaaaggaaga
     7441 gttttttact tcaattccta ttgaaaaata ttcttatatt aaagttggaa atgaagaaat
     7501 aaaaatttat tccttatggc caaatcttgt aagaacgaca actcttccac cagaaggtct
     7561 tgaaggaaaa cttttttatg ctggtaaagg agaccttaaa aatttagatg ggaaagatat
     7621 tgaaggaggt attggtgttc ttgattttga ttctgatgat agttggacca cacttgcagg
     7681 atttggagta aaaggattta tatttttaag ttctccagat atagaaagga aagaagcaga
     7741 aaagaagttt ttaagaatac cagttaatgt tcctaggttt tatgcagggg aaaattcaga
     7801 taaaataaag gcacttgcaa taaaagaaga aaaagttaag ttattttcaa aagttgactg
     7861 ggaaaatgtt cctaactaca atatttatgg aacaattgaa ggtaatgacc caaaactcaa
     7921 agatgaaata attcttttag aaagttatta tgatagtata agtgttgtac cctctattgc
     7981 tcctggggct acttctgcat gtggtatctc aacactttta aaacttgcag agttttttgc
     8041 aaatagtcat cccgaaagaa ctcttgtttt tcttgcaact tcttctcatt ttgaatatct
     8101 taagggtata gataatttta ttcagaatca ttcaagaaaa gaagaaccat ttttttcaag
     8161 aattcccaaa aaagagaaaa tattaccagc cctttctatt tgtttagaac tttcagatgg
     8221 ttcagatgaa ctcgcaatat ggcataattc ctatgatttt tataaacaga gatattttgc
     8281 tccatttgct aaaaaatttc tttcctattc agaaaaagtt tgtacaaaac ttggggttga
     8341 cccacaaaca gcccttatga atggagtaag tcctgaaaaa ggaataatat ggcaaacatt
     8401 tcttccagaa gcaataagaa ccgatggcga gtacctgatt ttatcaggaa accctgcaat
     8461 ttcatttgtt actgcaaatg atggaagatg gagagttgat acacctgttg atacatttga
     8521 gaatcttaat ttagaaaata tcgaaaaaca atttatattt cttcaaaaca ttctggatat
     8581 tgcattaaat gacaaggact ttttccctga tacagaactt gaattaaaag atactatgtc
     8641 aagtttaatt ccaaaaattg taacatttaa tcctaaaaaa agttttgtac caaacgaccc
     8701 tgttaacgaa ggtcttgtag ttcctcgtat gatttattat aatcctggcc tttattcaat
     8761 gaaaacattt ataggggtaa gaggagacat tattgagatg accaataaag atggtgaagt
     8821 taatatttca agattctcaa aattctctcc accactagaa gtttatattt atgcgttttt
     8881 cattgataaa aatacaggag aaataacttt aacaactgac cttggtgtaa atggagacca
     8941 acagtatcct attaaaacct tgattgatta caaagagaaa aagaaaacaa ctgtgctttt
     9001 taaatgtaaa ccaatatcat tatttggatt aattgaccct cagtatcttg tttcattaga
     9061 taaattagat gtttttgacc tttcgaattc tactcctgat gcatacgggt actatcttga
     9121 atatccatta tttattcctt ttatctggtc atctgattct gaaccagccg gttgtatttt
     9181 tgcaaaattt aattcaaaaa taaaagtaat tggagaatca ggaccattag gcaagcgact
     9241 tctccttctc aattcaaaac aaacattaaa taacaaagaa gatgcagaag gacttggttt
     9301 tgatgtagat agtattgagg caatttacaa tattccttat cagggcgcaa aagatatgat
     9361 aatcctggat acttatagaa gagtaaactt tgaaaagtat gggattagaa atgaaagatt
     9421 aaaggactta caaattgcct caaatgaatt tttagaacaa gcaacgaaag caaaaaaaga
     9481 gaaaaaatgg tatgattttt taaaattttc aaggcaggcg caggcaattg aatcaagagc
     9541 atacccagat gtaaaaggaa ctgctaatga tgttgttaaa ggtattattt tctacttcat
     9601 acttttgctt ccctttgcat attttgcaga aagattgttt ttttcctttt caaaacttga
     9661 acagagaatt gccggtatat caggaatatt tcttgtggtg tactggataa tgcgacttgt
     9721 tcatcctgct ttcaaactca ccaacgcacc agaagtaata cttctttctt ttattgttct
     9781 tgctctttct gttgttgttc tttcagttat tacttctaaa tttgaagaac agatgcagag
     9841 aatgaaaaga gaaacatcca atgtttatga aacagatgtc ggaagaataa ctgctactac
     9901 tactgctttt tccttagggg tagcaaatat gaaaagaaga aaagttagaa caatgctcac
     9961 agcaattact ttaatacttc taacatttac agtactatct tttacttcaa taaaatcata
    10021 tcttaaattc aaccaattat taagaccaaa tactccacca tacacaggaa ttttattaag
    10081 agaccgctca tgggctcctt tacaaaaatt tgctcttcaa tatacggaag atgagttttc
    10141 ttcaaatagt attatttcac caagaggatg gtatgtcctt agccaattag gaaataaaac
    10201 tgctgtggaa gtaaaatgtg gtgatgaaat atttctttct tctggaattt tagggttatc
    10261 tcctcaagaa agtgaaattt ctcctattaa agacacaatt atttctggga aatggattga
    10321 ggggaacagt gaaaataatg ttttaatttc taaaaaaata gcaaatgcac ttaaattaac
    10381 aaacgaagat attggaaaaa cattgaatat atatgggaaa caatttactt taataggaat
    10441 atttgacggg aaaaaattag gacagataaa agatatggac gatgaaccaa ttactcctgt
    10501 tgatttttct tctttacctg agaaagaagt aaataaaatg aagatggaaa aatcagctca
    10561 ggtttatact tcacaggcaa aattacagac atttacccat gtagaaggtg aaaataccat
    10621 tatattgcct tataaaacac ttttaaaaat gggtggtact ctgcactcaa ttgctgttaa
    10681 attttctcca gaaattacct cacctgcaac tactgttgaa aacttcatta caaaacttgc
    10741 tattattctt tttgctggct atgagaataa aacatttgta tatagcagta tgggaatgac
    10801 atcctttgct ggtttaacta atttgataat tccaatttta attgctgctt taattgtact
    10861 taatacaatg ttaggttctg tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10981 ntaattccaa ttttaattgc tgctttaatt gtacttaata caatgttagg ttctgtttat
    11041 gaaagattaa gagaaattgg gacttatagt gcaattgggc ttgctcctgt gcatattgct
    11101 tctttatttc ttgctgaatc acttgtctat gctgttttag gtgcagttgc aggatattta
    11161 ttaggacaga ttctttcaaa aattttaatg gcaacagacc ttttaaaagg gcttgtttta
    11221 aattattctt caatgtctgc tgtattttca acaataataa tatttataac tgtaattctt
    11281 tcaacaattt atccagcaag aaaagcatct caaatggctg ttcctgatgt cacaagaaaa
    11341 tggattctgc ctgaaccaag gggagataag tgggaatttg aatttccatt tacagtaagt
    11401 caatatgaag ttttaggact tgttacattt cttacagaat attttaattc ttttcaggat
    11461 gtttcacttg gaaatttcta tacaaatgga gcaaaactta tatatgaaaa acatcaatca
    11521 ggaaagaaaa aatatatttt agaaacagaa atatatcttt caccttttga ccttggagta
    11581 ggtcaaaact tcaaaatggt tttagaacca ttaggacaat atgactttta tactattaat
    11641 ttagaaatgg aaagaaaaag tggcgagtca aatgattgga aaagacttaa cagacgattt
    11701 cttgataata taagaaaaca atttctaata tggagaactg ttagttcaga aataaaaaag
    11761 gaatatacaa aaaaagggaa agaactttta aatctataaa tgtaaaatgt aaagagtaaa
    11821 gtgcgttttc cagcatccgt cactttttgt ggaggatgta aagttaaaat gtaaaattga
    11881 aaacgaagaa gaaaaattta aaattttaag ttataatttt aaactttaca cttttagttt
    11941 tgcattatta agttgtaatt tgctacttaa aaaggaaggt ataaatggaa aataaaatta
    12001 aagatgactc ctatcaggta gaaattgaag aaccatttca agagggattt aataagaaaa
    12061 cagttctggc cgctcttttt attggatttt tgatgcttcc aggttcaata tatttaggac
    12121 ttattacagg agttggactt ggaggtgctg ctcaatgggt aacagttatt cttttagttg
    12181 aaattgcaaa aaggtcattt gttcaattaa aaaaacagga agttcttatt atttatttaa
    12241 tagcacattc tcttgttgct gctggaatta ctatcggaac agcaagttta gttttacagg
    12301 gaggtgcttt tagtgattta atatggaaac aatatcttgt tcagtcacct tatgcagaat
    12361 catttggttt aacaccacat attccttcct gggcagttcc accagctggt tcagaagtac
    12421 ttcttaaaag gacattttta agtagagttt ggcttattcc aattttactt atcataattc
    12481 acaatgtact ttttagaatt aactattttt cacttggcta tatacttttt agaattacaa
    12541 gtgatagaga aaaacttgca ttcccaatgg ctcctgtagc atcagaagga gcaatagcaa
    12601 tggctgaagt aagtgctaaa aaagatacat ggagatggaa agtttttagc atatcagcaa
    12661 tgataggggt aatatatggc gctttttata ctgtaattcc aactataaca ggacttttaa
    12721 tgacaaaacc atttcaaatt attccaattc catggcttga ttttactgaa aaaattgggt
    12781 taatttttcc tgctgctatt ttgggatttg taactgacct tggttctatt tttactgggt
    12841 ttgttctccc attttgggtt gtatctggga catttattgg ttctattgtt gccagtgtcc
    12901 ttttaaatcc tattctatat aaaagaggaa ttataaaaaa ctggcaacca ggtatgactg
    12961 ctgtgcctgc aaatgttgta tcacagatgg atttctgggt aaatattatg attgctggag
    13021 caatagttgt tggtattatt ggtgttgtta gaggattggg taattttata aaaacaaaaa
    13081 atacggtaac aacagaaaag aaacttcccg ctgggagagg tgattatcca atacctattg
    13141 cttttggtat atggtttatt gctactttaa tttacataat aatatgccat attcttgttc
    13201 ctaaatttcc tgtaattctt tttatatttt ttggttttat tttaactcca tttttaagtt
    13261 atacttctgc aaggatgttt ggaataacag gagtagcagg tggaagtgat ttccctatgg
    13321 tcagagaagc aacatttata ttaagtggtt ataaaggtgc agatatatgg tttgctccag
    13381 ttccatattt cgttattgga gggcaggttc aaacatttaa acaattagaa ttaacaagaa
    13441 caaaatttac ttcctggtat aaaggtgagt ttactgcact tggcataatg cttttttgta
    13501 gttttctttt ctggtcaatt atatggagaa tgaacccaat accatcttca acatatcctt
    13561 ttgttcaaaa aatgtggccc atgagtgcaa tattccagag tttatgggca acaagcactc
    13621 ttgaaacagg acagaattgg atgattaaag caataaaact aaaatatatt tctggtgggg
    13681 caggtattta ttttcttctt tacttcctat ttactatttt caaattcccc attttcatgt
    13741 tctatggaat tattggaggt gttgccatga tgccccatta tgttctacca atgtttttcg
    13801 gtgctctcct tggaaaattt tatttttcaa agaaaattgg taaagaaaca tggagaaagt
    13861 atacaccaat aattcttgct ggttatgcat gtggtatggg acttataggt atgatttcag
    13921 tcgcagttgc tcttattgca aaaacaatat ttcaaataat cttctaaata tttaattcct
    13981 ctaaataatc agggacatat attgaagagg acaatttttc ctttaaaaaa gaagaaaatt
    14041 cacttataac ttcttcttct ccatgaataa ggaatatatt tttcacattt ttaattttat
    14101 ctatccagaa aaatagttca tttctatcag catgggcaga aaatccattt atactttcaa
    14161 ctcttgcttt taatggataa aattgtccaa gtattctaat gttttctttt ttttcaagta
    14221 tttctcttcc aagcgttcct tttgcttgat aaccaacaaa aagtattgta cattcttctc
    14281 ttgttatatt tgttattaaa tgatgtttta ttcttccacc agtacacatt ccactacctg
    14341 ccattataat tgacgaactt tttatatgat ttattgcttt tgattcatta acacttcttg
    14401 taaattttag aagtggaaaa tcaaaaggcg aatttccctg tttgaacatt ttccttgttt
    14461 cttcgtcaaa atattcagtg tacttttcaa aaacctcagt aatactaatt gccataggac
    14521 tatcaacaaa aacaaaaata tgagggattt tatcttcaat taaaagtttt tttaaaaaat
    14581 aaagcaattc ctgcgctctt tcaatcgcaa aagtcggaat tacaacattt cctccttttt
    14641 gaactgtttc attaataata tctccaagtt tttctcctcc ctgattttca ctttcatgta
    14701 tccgattacc atatgtgctt tccataaaaa caatgtctgc ggaagaaaaa aaagtcgggt
    14761 cttctaaaat tactttattc catctaccaa tatctcctga gaatattatt tttttaattt
    14821 ctcttccttc tttaattacc agttctacca tagaggatcc taaaatatgt cctgcatcat
    14881 gaaatgtaaa acttatatta tctgaaacta taatttcttc tctatatttc tttggtctaa
    14941 ataaggaaaa aacattttct gcgtcttcaa ttgtataaag tgggatttca ggaaatggag
    15001 ttattcttct ttcttcctgg tgtctaattc ttttcttttc tgcatcttct tcctgtaatt
    15061 tcgcagaatc aagtaaaaca attcttgtaa tttcttctgt tggatatgta caataaattt
    15121 ctcctttaaa tccatcttta acaatttttg gtaaataccc acaatggtca agatgtgcgt
    15181 gagttaaaat tataaaatca attgtatcag gactaacagg taaatcctgc caatttctat
    15241 ttttaaactc tctctcttgg aaaagaccac aatcaataag aaaccttttt tcttttgttt
    15301 caataagaaa ttttgaccca gttacatttt ttcctgctcc aagtattttt aatttcatta
    15361 cattcccctt ttaattataa cttcattgtg cccctggccc gacttgaacg ggcgacacgc
    15421 ggtttaggaa accgctgctc tgtccatact gagctacagg ggcatatttt cgtaatataa
    15481 ttacaactga aagtttgtaa ataaaaaatc caattttgat tttaccttat ttttatttat
    15541 tttaaaaata ctgaattgag atatattaaa tttttatatg ttgaacactg gtatccttgc
    15601 gcaaaaaatt ttaggaaaga agaatgggta aaataaaata gacacgatgg tagagtagaa
    15661 agttcaaaca gaaatttttt ctttagtttc tatggggaga agtttatcta aatcaatatg
    15721 ttttgaagca gaaagaattt caataccagc aattttccct ctttcatcca tatcaattgt
    15781 tatatcatct gtaagatttt tacatactac ttcttttata tctttaaatt tcaaatacaa
    15841 aacatcatat ttactatcat aagttatttt cattttctat cctcccattt tccaaaatat
    15901 acataaactg taataactat caactggttc ttttcttctt tatatattac cctgattttc
    15961 ttttgagagt aaaatttatc ctgccatttt ttcccatagg gggaaaccat ctcttttgcc
    16021 tgtatggaat atctaacttc atatctttgc ccttttttaa ttgtttcttc aacttctgaa
    16081 atatttgctc ctctgttttt aattctatcc ctggcgtgtt ctataattat aattcttttc
    16141 cccattttta tattttaata catttgggga aaatttgaaa agaaaaattt cctctgtgcc
    16201 aaattggagt gtaaaagaat gaaaaattat gaaatatagt gaagaaaaaa aatattttaa
    16261 atgcttatcc ttctttgttt tccttaattt tctttagttt ttagattttg gttttttagg
    16321 tttaggaaac cgttgttctg tccatactga gctacagggg catattttcg taatataatt
    16381 acaactgaaa gtttgtaaat aaaaaatcca attttgattt taccttattt ttatttattt
    16441 taaaaatact gaattgagat atattaaatt tttatatgtt gaacgctggt atccttgcac
    16501 aaaaaatttt ggtagagtga aggggaaaaa ataaaataga cacgatggta gagtagaaag
    16561 ttcaaacaga aattttctct ttatttgcta tagaaatttg ttatataata catttctaga
    16621 acaaccaaat aatttttgat aaagagaaaa taataatatt catataaata ttaaaaagga
    16681 gaaaaaatgg gtattacaat aagaagagaa ttgtatgtaa aagcacagga taaaaatatg
    16741 tgtgaatggc gttctcaaaa atatgtcaat ggggaaggac taaaaagagt ggaaattaga
    16801 agtttagaag gagaatccga ctggagtagt ggcttatttg agagatattc tgatgataat
    16861 gggcgtacat ggagccaatg gaaagatata tattcacagg gatatgaaaa aaaaggagaa
    16921 gatgaaattt ccactaacta tggctgtgaa acatataatc cacaatataa acattttgta
    16981 tcacttatga tgaacaggat atttttaggt ggacatactc aggcatacaa aagattttgg
    17041 gaaaagggcg aagcaggatt tattgaccat tgttttctat cagttcgtaa cgataaaagc
    17101 caaaatagtt cagtgaaact aattaaatat gaagaagggg ctgattatga tcctgataac
    17161 tggcttaacc cggaatatat cactcgtaat cgtgcttatt ttggatgtaa tattgatatt
    17221 ttgaataatg gagaaataat tttccccatc ggtgctaatg ttaaggcatg ttgccgtatt
    17281 ctctccctaa acatcaatga tatattccct tcctgcccag atattatgtg tggtatgatt
    17341 gtagtgcgtg gtatatttaa ccaatcttct ggaaactatg attttatatt ttctaaacca
    17401 gttgttataa gtgatttgaa atcttccaga ggtattgatg aacctgcagc aatatccctg
    17461 ccatcaggta gaattattgc tgtattccgt ggaagtaatg ttgaatgcaa ggattggaat
    17521 actcgtatag aacctggcac tccttcacat aaatggtatt gttggtctga tgatggtgga
    17581 aaaacattca ctgaccctgt tccatggcac tttgataata gagaggtttt ttactctcct
    17641 gcctctattt ctaattttat caaaagtata agtaatggga agatatattg gattgggaac
    17701 attactggat ataatgctta tggaggacat cctcgttatc ctctggtaat agcagaagtt
    17761 aatgagagag ggctacttaa aaaagagaca ttgactataa ttgatactag gcaggaagga
    17821 gaaagtaaag aagtgcaact ttcaaatttc agtattttac aggatagaga aacaggactt
    17881 atagaactct acctaacaaa aataggacaa cacgaaggat atacatggtg ggcagattgt
    17941 tatagatatt ttattgatgt taataaataa gggatttcgt cacattttaa acatttaata
    18001 tatcttcttt aacaaaagta attttttctc tatacattac aagaaataat cagagagaat
    18061 ttttgttttt ctctcccttt ataaaatcct atcatgattt ctgaaggagc aggtcggaat
    18121 gaatttattt attgttttta ctctatgttt ttgtgtccaa tggctccttt ggacctaata
    18181 tatgggtttc actaaggtct tacaatacat ttggggaaaa tttgaaaaga aaaattttct
    18241 ctgtgctaaa ttaaaaaagg aaagttgacc agaaatacct tataaagtaa aatttaacta
    18301 ctatggacat gaataaaatt gaggaaaaat ttgaaattat taaaaaaaat actgttgaaa
    18361 taataaatga ggatgaatta aagaagaaaa taagaaagtc aatagaggaa aaaagaccat
    18421 tacgggtaaa atatggaatt gacccaactg ctcctgaaat acatttaggt catacagttc
    18481 caataaaaaa actaagggac tttcaaagat tgggggataa aattctattt cttattggtg
    18541 attttactgc aagaattgga gacccaactg gcagaaaaga aacaagacct atcctctctg
    18601 ttgaagaaat taaaaaaaat ttattaagat atagacaaca ggttgcaaaa ctccttgata
    18661 ttgagaaagt tgaatttgtg tataattcta catggcttgc taaacttaaa cttgaagaaa
    18721 taatagaact gacatctatt tttactgttg ctcaaattct tgaaagagat gattttacaa
    18781 aaagatataa agaaggatat ccaatatatc ttcatgaatt tttttatcca cttttacagg
    18841 gatatgattc ttatcattta aaagcagata tagaaattgg agcaacagaa caaaagttta
    18901 atttacttgc aggaaggaca attcaggaat tttttggaca gagtaaacag gttgttatta
    18961 caatgcctat tttagttgga atagatggaa aattaaaaat gagtaaatca tatggaaatc
    19021 atattccaat agaaacaaca cctgatgaaa tgtttgggaa aattatgtca attcctgatg
    19081 acattatgaa agattacttt ttacttctta caggtatccc ttctgaaatt tttagtaaaa
    19141 aaatagaaga aaacccaaga caggcaaagt gttttttagc aaaagaaatt gtcagagaat
    19201 tttttggaga agaacaagca agaaaagcag aagataaatt taataaaatt ttcagagata
    19261 aagaaactcc ggaagaaata cctgaattta aaatccctgc cgaccttctt gaagatagca
    19321 aagtaaatgt cattgattta atatatgcct ctggaattat tagttcaaaa tcagaaataa
    19381 aaagattaat ttcccaaggt ggaattaaaa taaatggaga aaaagtaaaa gatactgatt
    19441 ttgtttttga agtaaaggac ggacaaatta taaaaatagg aaaaagaaaa tttctgaaaa
    19501 taaagaggtg aaaatggaaa atataagaga aaaactaact gaatatggta aaaaaattgt
    19561 agagaaaaaa cttgttgctg gctcaggtgg aaatataagt gcaagagaag gaaattttgt
    19621 ttatctttct ccaagcggtt tttttcttga tgatataaaa aacaacgagt gggtaaaagt
    19681 tgatataaaa acaggtgaaa tttatggaga gttaaagcca acatgtgaaa tttcaacgca
    19741 tctgggtatt tataaagaaa gagaagatgt aaaagttgtt tttcacactc acccaccatt
    19801 aacagttggg ttgatatcct ctggaatgga ctttaaacca ttttttccgg attttgtcgc
    19861 tgttttaggg aaagaagttc ctgtaattga ttatgttcca ccaggtggag aagaaatgag
    19921 aaaaagagtt gttgaaaaaa taaaaaattc taatgtagtg ctgataagaa atcatggtgc
    19981 tatttgtata ggagaaacat ttaaagaggc atttgcaaga gcatggctgg ttgaagatac
    20041 agcaaaaagt ttaattgcct cacttatatc tggtaaatta aggtatttta caaaaaagga
    20101 aattaaagga atagaaaacc ttgaatcaga agattacaga aaagcaatcc ttaaaaaaat
    20161 ggaataatga aagaaaaatg gaaagttgat aaaaagaata ttttattaaa aaatgtcctg
    20221 atagcaggga tattaaatat aacctctgat tctttttatg atggtggaaa atttattgat
    20281 attgaaaaag caattgtaag aggaaaagaa attataaagg atggagcgga tataattgat
    20341 ataggaggag aatcatcaag acctggtagt attccaataa gtgaaaaaga agaaattaaa
    20401 agagttatac ctgtaataaa agaactttca agtgaaaata taattatctc agttgataca
    20461 tataaatcaa aagtggcaga agaagcaata aaaaatggtg caaaaattat aaatgatatt
    20521 tctgctttag agatggatag gaaattagta aatatattaa aaacaagtga gtgtgggtat
    20581 gttttaatgc atatgaaagg taccccaaaa attatgcaag aaaatccata ctataaagac
    20641 acaattggtg aaatcacaga attctttaaa gtaaaattag aatatattga aaaaaatgga
    20701 attgatattg aaagagttgt aatagaccct ggaattggat ttggaaaaag agttattgat
    20761 aatatcctta ttctaaaaca tcttgataaa ttcaaaaaat ttaagagacc tatttttatt
    20821 ggaacttcaa gaaaagcatt tatagggaaa attttgaatt tagaagttga acaacgactt
    20881 gaaggttctc ttgcaacagt tgtttatgca tacttaaaag gaggtagaat tttcagggtt
    20941 catgatgtaa aggaaacaaa aagagtactt gatattatac aggcaataga aaatgagaat
    21001 attgggactt gatataggga caaaaagaat aggagttgca ataagcgatg aaagttgtac
    21061 tattgcaaga ggacttggaa ttcttgaaag aaacgggaaa gagataaata aaattaaaga
    21121 aataattgaa aaatataata ttgaaaaaat tatttatgga cttcctttaa ggatggatgg
    21181 ttcaataagt ggacaaacag aattagttat ttcctttatt tcaaaactaa aaaacgagat
    21241 aaaaatccct ttaaaagaat gggatgaaag attgagcaca aaaaatgcag aaacaatttt
    21301 aattcaagca gacataagta ggaaaaaaag aaaaaaactt attgataaac tttctgctca
    21361 aataatttta cagaattacc ttgattcaaa aacccttgaa aataaaacca ttgaggatat
    21421 agaacatgaa tattaacttg cttgataaaa tttttcatgg agtcaaaata aaaatacaaa
    21481 aagtaagaat aaaaaagtgt tattactata ttcccctcta cacatttttg actttttatg
    21541 tatgactggt tataattcaa aaaattaata atttagccaa ttaaaaagga gatattatga
    21601 gaaaaattaa atgcgcagtt gttggaacgg gtatttttgg cgaattacat gttgaaacat
    21661 atgcctctta cgataaggca gaccttttaa ttatttgtga ttcaaatgaa gacagagtaa
    21721 aaaaagtaag taagaaatat aatgtaaaat atactctgga ctataaagaa gttgcagaaa
    21781 atgaggaaat agaggcagta agtgttgcaa ctcctgattt tgctcataga gatatagttg
    21841 ttgaaatgct ccaaaagggt aaacatgttc ttgttgaaaa accaatggca acaaatgtta
    21901 aagatgccga aataatgctt gaagccgcca aagaaagtgg aaaaatttta atgattgatt
    21961 tccataatag atttaatcct gcgtttattc agacaaaaga gagatttgat aatggggact
    22021 ttggagatat tggaatgatt tatacaagaa tgagtgatgc aatatctgtc cctctaaaat
    22081 ggttttcctg gtcagacaaa agtggtccac aatggttttt attccctcat attgttgacc
    22141 ttgtaacatg gataagtgga gaaaaggtaa aaaaggttta tggtataggt aaaaaagaag
    22201 ttttaaaatc acatggactt gatatttatg atacagtaac agggattctt gattttggaa
    22261 agttttatgc aactgttgag acatcatgga ttatacctga ggtatggaca tcaatatgtg
    22321 atttttattt taaattagaa ggaagtaaag gaagagttga agcggatttt ggagatatgg
    22381 gattgaaaat agcatctgat gtaaaaaaga caatggaaat tcctttatat actggatata
    22441 tcccaactta tggaaaatta cagggatttc aaccactacc aattatgtat tttattgatt
    22501 gtatactgga aaataagaaa ccatttatta ctcctgaaga gggtttagaa aatgtaaaaa
    22561 taatttcttc tattataaaa tcagttgaga caggaaaagt tgttgaaatt taaggaggat
    22621 aaaaatgaaa ttaggaatag taacttatca gattgcggct gattgggaca ttgatacaat
    22681 aattgaaaag tgtaagaaat taggttatga aggtgtggaa ttaagaaccg gccataaaca
    22741 cggtgttgaa acaactcttt caaaacaaga aagagaggat attaaaaaga aatttcaaga
    22801 gagcaatatt aaacttgttg ggctgggaag tgtttttgaa tatcatagtc ccgataaaga
    22861 agaattaaga aaaaatattg aaggaacaaa ggaatatata attttagcca aagatgtagg
    22921 ggcagaggga gtaaaagtaa gaccaaatgc ttttcctgaa aatgttccaa gagaaaaaac
    22981 aatagagcag attggtatat ctttgagaga agtatcttct tttgctgccg attacgggat
    23041 aaaaatacga cttgaagttc atgggaaaga aacatcctat ccttattata taaaacaaat
    23101 gataaatata gcaaaccatc aaaatttata tatttgttgg aattcaaaca tcacagatat
    23161 tgatgaaaca ggttcaattg aaaaaaattt taacctgctt aaagataaaa ttgatatatg
    23221 ccatataaat gagttatgta atgaataccc gtggatagaa ctttttaaac ttttaaatcg
    23281 tataaatttt gatggatttt gtcttgcaga aattcaggca agttcagaac cagatagatt
    23341 actttcttat tataaagttc tttttaacac ttataataaa attgttcaaa ctgaaaaata
    23401 aaaaattatg cgaattgaag ttgccataat gtatagtaca ccccttgctt atcaagtagt
    23461 tgctgatgtg ttccttcttc aacaattgtt ccatctttaa gaacaattat tttattaact
    23521 ttttttaatg ttgagagtcg gtgagcaatt ataaaagatg ttctattttc aagtgtctgg
    23581 tcaattgctt tttgtatcat tttttctgat tctgtgtcaa gagaagaagt tgcctcatca
    23641 aaaattaaaa tatcagggtc tttataaatt gctcttgcta ttgaaattaa ttgcttctgc
    23701 cctcctgata aagttattcc tctttcacca acaatcgtat tttctccttc tggcaaagaa
    23761 tttattaact gcgctattcc atccattttt attacttctt taaacctttc ttcattttca
    23821 aatccaagtg ttatattttc tttaactgtt gaatgaaata aaactggttg ctgtgaaaca
    23881 aaaccaatat gctgtcttaa agaatttatt ttatattctt taatattttt cccatcaatt
    23941 aaaatttcac ctttttcagg gttataaaat ctcaaaagaa gtcctactaa agttgttttt
    24001 ccaacgccag ttggcccaac tatccctatt ttttcacttt tttcaactat aaaacttatt
    24061 ccttttaaga cctgtttccc ttcaaaagaa aaaaacacat ctttaaactc aatatttttt
    24121 ttaagtccca taaagacatc tttcccttct tcaacaactg aactttctgt atcaagaacg
    24181 gaaaatactc ttggtaaagc agaacttgcc tgttttatca atccatatgt ctctccaatt
    24241 ccttttaatg gacttatcat tgaaagaaga gcaacaaaaa acaaaaataa aaagccagta
    24301 ctcattcccc catttattat tttaactgct ccgtaataaa taagtccact tgatgcaact
    24361 gctcctgcaa attcagtaaa tggagatatt ataagcaatc tttttaccat acttattaca
    24421 tttttaaata tattttcatt ttccttctta aatttctctt tcatatattc ttctctgttg
    24481 tacaccttta taatttgctg tccatataca ctttcattta taacattacc aatttctcca
    24541 tatgctatct gtattttttt gcctaatttt cttaatttat ttcccatttt aaaaacagga
    24601 taaagaataa cagggaacat aaatattgaa agtagactca ttttccaatc aattgtaaaa
    24661 attattacaa agtaggaaaa agtgagtaat attggaaata caaatttagg gaaaaatgat
    24721 tcaaaaacaa catttagaag gttaacatca taaattattc ttgttgttaa ttcacccgtt
    24781 tgtttttttg caaaaaaatc atctgataaa ctcataattt ttgtgtaaag taattccctt
    24841 acatcagtca ataaccttgt tgagaaacat ctataaaaat aattctgtaa ataaaaaaat
    24901 agccctttaa gaaaaagaat agttattaaa aaaataagaa ggtatttcaa aagtaataag
    24961 gatgggagtt ggtttatttt aaaaatcaat ggttctattt taatttttat aatttgaggt
    25021 aaattttctg gcagaataat tttttctttt ttaacaagga ctttatccat taaaggtact
    25081 attgctgaaa gagaaacacc attggcaaaa acagaaagaa ttgtaaaaaa gatacctgcc
    25141 acgagtaagt gccacctaac ttttacataa tttttaattt taaagaattc ttttatcatt
    25201 ttttcccttt tgtcagttct aaaataaatt ctgcaacttt tttgcttaca ccttcctctc
    25261 caattttttc ttttaaatat ttaaaacttt taatttcttt ttgatatatt tcttttttac
    25321 caattaaatc aataatatct ttcttcacct tttcttttgg tattttttgt atatactcag
    25381 gaacgacatt ttctcctata aaaagattaa ctggacttac catgtttaat tttaccatac
    25441 atctaccaat aaaataattt aaaaaagatg ttttataaaa aacgattagt ggtttcaata
    25501 aaagagatac ttctaaatta acagtgccac ttgtagataa aattaaatct gaattttcaa
    25561 ttaaagtgta cgggaaacct ttaaaaattt caaactcaat tctttttctt ttacatattt
    25621 tttttataac catttctatt tcctcatcaa aagatgaaat accaacatca aaatcaattt
    25681 cttttttcaa gtcataaaca atatctaaaa ttacaggtaa tgtgtaagaa acctcctgta
    25741 ttctacttcc aggtaatatt ccaatcatct tcttatctct ttttctatct tctccttttt
    25801 tgataaggtc tacaacagga tgcccaaaat atgtacattt tatcccttct tttttatata
    25861 tattctcttc aaaagggaat attggaataa caaaatttat atatttttta atttttttta
    25921 ttcttttgta attatgagcc caaatttttg gaggaatata atagacatta aaaaatttcc
    25981 ttttaagttt ctctgcaact ttaagattaa aagcagggtt atcaataaaa acaattatat
    26041 ctggaattct tttttcaatt tcataaataa tttttttaaa aaattttaaa tatgagggga
    26101 gttttaaaat aacagaagaa tatcccatat atccaactgg catgccttta ataatttcaa
    26161 caccaacttc ctcaagtatt tttccacctg tcccaaaaat ttcaatatca ggatttattt
    26221 ctttgatatt tttagcaaga attgctgcat aattgtcccc tgaaacttct cctgtaaaaa
    26281 tccctatttt cattttaaaa tggatgagaa ataccaaaaa gataaagatt tgagttaatt
    26341 cctgaatttg gatggtctat tcctgcattt gaaatatgtc tatatctata ttcaagaaaa
    26401 atgatatttt ttttaaaaga aaacttaata cctccgcata tctggtcaac aaaattaaaa
    26461 tttgttgatt gttcataagt atcctgtgaa atatatataa ttccacttcc accttttata
    26521 tacggagaaa tccttttatt tctaaaaaaa ttgtatttaa agaaaaaagc aagtccaact
    26581 tcaaaattgt tttctggctt gtaaacataa gaaacaaaag gttctaactg aaaatcccag
    26641 acatctgata aaggataaga aaaatcaact gcaaaaatgg gaaattgata atcgggttgt
    26701 ctttcaagtt ttccttctcc ataaccaaca aaataatcaa tagaagaagg tttttttata
    26761 tcaaaagcat aaacattaac aacaaaaaca aagaaaccaa atattaaaat tctttttttc
    26821 atagaaaaat tctaaaacaa tattttataa ttgtcaaact tctctttatt taacaattat
    26881 tttgttgaca taaaaaaaga aggttgttga tataaaattt aaaaaaattc tacttttaat
    26941 tactttatga ttctgctatt tcttattata ctgattataa gatatatacc aaaaattcca
    27001 cttacaataa aaccaactac tccaaatgca gaatgtcccc agagtttagg tccttttcct
    27061 gagaccataa taagagatga tgaaacaaga attgcagata ttattaaact aaaagcaact
    27121 ctactacttg atttatcaat tgttgaagtt aaattctgta atcccttatg ttcaaaagca
    27181 acatttatat atcccttttt caacattttt aaagatgcct caactgtttc cggtaattct
    27241 tttaatatgt cataagtgga tgaaatattt cttaatgtat cctttaatat ccctttcact
    27301 ttttcttttt tttcaataaa atcaagaaca tatgtctgta aatattcaga aatgttatat
    27361 tcaggatata taatagtagc aacgccttca agagttaaaa ttgcttttgc aagtaaagaa
    27421 aaatttgctg gaacagatat tttatacttt ctcataatag aaaatatttc atcaaacaag
    27481 acatcaatat taatttcccc taaagatatg tctctatatt tttcaatgta gtcatctatt
    27541 tctctttcta attttctttt atcaatctcc tctgcttgat aacttcccat tgcttcaagt
    27601 gttttaatta ttttttcgct atttgcttta atagcacctg ataataaact tataatttgt
    27661 agtttttctt cttctgtcaa tctaccaaca attccaaaat caagaagaca aaacttacct
    27721 tcttttgtaa ttaaaatgtt tcctggatga gggtctgcat gaaaaaaacc atcttgaaaa
    27781 atctgtttta atattatttc tgttccctgt tgtgcaagtt tttttctatc aatactatta
    27841 attttatcaa tttctgttat tttaactccc tcaatgaatt gtaaagttaa aacctttgtt
    27901 cctgtaagtt ctttataaac atctggaatt attatatttt cataagaaga aaagtttttt
    27961 ctgaatcttt gtatattcct tccttcatat gaaaaatcaa gttcctttct tacactcttt
    28021 tcaaactctt caacaatttt tacaggtcta taaatacttg cttctttaac aaatctttca
    28081 attaattgtg caatatcata gagaatctcc atatcaagaa atattttctc ttcaatttct
    28141 ggtttttgaa tttttactgc aactttttct tttttataag aagcaatatg aacctgtgaa
    28201 attgaggcag aagcaattga cttttcatca aaataatcaa atgtatcttc aattttacat
    28261 tttaaatttt tttcaagaac atcttttatt ttttcatatt caactggttc tgttgagtcc
    28321 tgtaattttt caagttcttt taaatactca attgggacaa ggtcgggtct tgtacttaaa
    28381 atttgcccca tttttataaa agtagggccc aattcttcaa gcatttttct taaccgaata
    28441 ggaggggata atatctgatt tttttttcta atgggtaata ttgataattt tatgtgggct
    28501 ttttcaagca aatatccaaa tccatatttt attacaatat ttgcaatttc tctgtatctt
    28561 ctaatttttc taccaatttt atatcttgcc atgtagatat catttaaatc cgttcatatt
    28621 tcaagaataa aggcacaaag ttcaaaaact tttcttctca tcttgaatcc ctcgggtata
    28681 gttcagagaa attctgtgaa ccatacggac aactctcgcc ccaaagggga gaggctcgcc
    28741 cgtagggcta cgcccgaggg gttagggtga gggggaattt ttacttttgc cttctcagtc
    28801 aaaacatttg actgggtgtc ttgccattac tcacttttcc caagatactc gtttttccag
    28861 agcggttcaa caagttctct gtattcttta ttatattggg gcattttccc ctgttctata
    28921 aaagataaat attcttttgc tccttcatca agaggaactg ctcctgtttt cttaacaatt
    28981 tcataaaaat cagttgaatt gtctcttata atacaaggag taagcaaatt tccacattga
    29041 caggcaggcc tttcaatcca gtaattattc tgccattttc ttatttcttc aaaaagtggt
    29101 gattttattg catcagttat tgttctgcct tcattataaa gttgataaag atttccataa
    29161 tttttatctt taaatggaat aaatacacaa ggagttatag tgccatccca cataatataa
    29221 aaatatcctc caccacgagc agcagccata catccatcag aagcagtccc tgaattccag
    29281 aagtcggcta taaaaatttt atcttcaatt acaagtttcc atattctttt caacatttta
    29341 tatctctgtt caggtgttac catcaactca acagatggtt ttcttccaat tggcatatat
    29401 tgaaaatacc atccataaaa agcacccttt ttattaaaat aatagtcaat aaattcatct
    29461 gataaaagaa gttctgcatt atatcttgtt ggagttactg atattccaaa aggtactcca
    29521 ttttctttca aattatccat tgcttccatt atttttttat aaatgccatt tcctcttctt
    29581 tcatctgttt ctttctcaaa tccctcaact gaaattgcag gggtgaaatt cccaagttca
    29641 gataactttt ttgcaacatc tttttttatc atagttccat ttgtatatgc cataaaataa
    29701 gcatcattat gtttttcaag tatatcaaat agtgttttcc cattttctct gtacataaat
    29761 ggctctccac cagaaataac aaaaaaatat gctccaaatt gttctttaat ttctgaaata
    29821 actttatcaa aaacatcaaa ttttaaagta tcttttgtgt aaatttcacc tgcataacat
    29881 cctttacatt tcagattaca tacatttgtt ggagaaatag ttacaaaaca tggtggtcta
    29941 aatccatgtt tttcttcaaa agttcttctg acataaatac cttcaatgaa tatcttgcca
    30001 agaaaaatat ttgaaagtgc ttttacacaa tttttagaaa tacttcctga atcaaatgcc
    30061 ttttgtaatg aacttaaaag tgcacttccc atataatatc tttcttccat tactttgcgt
    30121 ggtctatcag gacctttaaa tgccattttg taagtcgctt tttcaatagc atttattaat
    30181 gcccttcttg taacaggtgt tttaagtccc atatttataa ttcctgctac tgcttttctt
    30241 gaaccaccct tctccattat attttttact ctttcttcaa gtgttaacat actgcctcct
    30301 tattttctat tattatacca cttattataa ttttgtgaac tattttatta ttttgtgagg
    30361 ttgatttcaa gaaatgtttc ttctctctcg ctgattgtaa attttaagtg atttttgtaa
    30421 atgggttttt gaaataggta tgagtaaaca tttatgattt gtgttataat ttttaaaatg
    30481 gaaataaaaa attgtaaagt tattaaaaat caaaagatag cagaaaaaca ttttatttta
    30541 aaatttgaaa ataaattttc aaaagaattc cttccaggac agtttcttat ggttgctctc
    30601 cctcactttt atttgagaag acctttttct gtttttaaag tcaataaaaa tacaatttct
    30661 atattatata aagtagttgg aaaaggaaca gaaattttat caaaaatgaa aaaaggaaat
    30721 attttaaata ttttaggtcc ctgtggaaat gagtttaata ttgaacaaaa atacgaaaat
    30781 atctggatta taggaggtgg cactggaatt gctcctttaa tttttctctc agaaaaactt
    30841 aaaagaaaaa ataaaaatat aacctttttt tatggagcaa gaaacaaaaa cttgttattt
    30901 tttgatattt tacctgctgg aattgaatat gtattttcaa caaatgatgg gagttatgga
    30961 tataaaggaa cgattttttc aattgttgaa aaaatgataa aaaaagaaga aaaaccagat
    31021 gttatttatt gtggtggtcc tgaattactt ttgcaaaagg tatcttcatt atcagtgaga
    31081 cacaaaatcc catcatttat aacccttgaa aattttatgg gatgtggaat gggtgtttgt
    31141 tatggatgtg taattaaggt aaaagaacaa aatggctggg agtataaaag agtatgcaaa
    31201 gatggcgcta tttttagagg agaggaagta atatggcagt aaatttaaat acagaacttg
    31261 gaaaattaaa attaaattca ccaataatta tggcaagtgg aacatttgga tatgggactg
    31321 aaaaatttca atttattgat tataaaaaaa taggtgccat tacaacaaaa acaataactt
    31381 atgaaaaatg ggaagggaac ccacaaccaa gaatttctga aataccatct ggaatgatta
    31441 acaatatcgg acttcaaaat cctggtataa aatatttttg tgaaaaaata tatccttctc
    31501 ttgaaaaatt aaaaaaagag gaagtgaaaa tttttgtcag tataactgat agaactcttg
    31561 ataatattat aaaaatagtt gaaaaattaa attatttaaa aattgatgca atagagttga
    31621 atttttcctg cccaaatttt gaaaaaaata atttgatgat ttctcaaatt cctgataaaa
    31681 cattttcagt tgtgaaagaa acaaaaaaag tgagtaaatt tcctgttata acaaaacttt
    31741 ctccaaatgt aacagatatt gtagaaattg caattgctgc ttcagaaggt ggaagtgata
    31801 ttttatgcat ggtaaataca gttaagggaa ttttttttga ctggggaagg aaaaaaataa
    31861 ttgaaggggg aatttcagga caatgtataa aaggtattgg gttaagatgt gtttatgatg
    31921 tttataaaag agtaaaaatt cctataattg gacttggggg gattgtttgt gggaaagatg
    31981 ccttagaata tattttaata ggggctgatg catttggaat tggtagtggt tttttttcaa
    32041 atccaggaat tgttaatgat gtttttaatt atctttcttc ttatcttaca aaaaagggaa
    32101 ttaaaaatat aaagcaaata gtaggtattc tgaatgaaaa aggaaaataa aaaagaaaag
    32161 aattattttt taagtatttt aacaatttta ttatgtctat ttattttttt aagggtgtgg
    32221 tatgacggaa taagtgaacc atttataaat accctccttg ttttttatat tttcttttta
    32281 accctaataa gttatttata cagaaaagaa aaaacagaga atttaccttt gatgtacttg
    32341 ccttttttcc tttttgttct attttctttt atttcttcag gtatttctca aattaaaggt
    32401 agtggtatta cttataatac aaatatttta tctttttttt gtttgatgat tcttattagt
    32461 aaaattgaga agcaacaagt aaaatatcta atttatactt tattttttgc ctgttttgtt
    32521 ctcggtattt acgggattta tcaatattac tggggatttg aaataacaag acaatatatt
    32581 ctttctaaca aagaagtttt agccaattta cctccaacat ttcttgcccg acttgaaagc
    32641 aacagaattt tctcaaggtt tgtctacccc aatgtttatg cttctttttt attgacggtt
    32701 cttcctcttt cattttttac tacttttgaa aaaaattttt atataaaatt tttttcctgg
    32761 gccacttttt ttattgcttt ttataatctt ttactcactg gttcaatagg gggtattctt
    32821 atttttattt ttgtttttca ccttattttt ctgtttttaa tcctaccaga aagaaaattt
    32881 tttaaaatca cccccttttt aattcttttt gaaataatat ttgttatttt tatttacaaa
    32941 gcagggaaat tacctcatat tcattctttt gttgatagat taaattactg gaaaagtagt
    33001 ataaatatat ttaaagaaag tcctatttta ggagttggac ctgaaaattt tgcctttttc
    33061 tttacaaaat ataaattacc gcaaagtatg gaagcaaaac atgctcatag tttatttttt
    33121 gaaacacttg ttgaaaacgg tattattggg actatattcc ttttttcttt tgtattttcg
    33181 gtactttata atattttcat caaaaaagag aagcaaaagg atttttttct atatggaatt
    33241 ggattttctt ttctttcttt gttaattcac aacattattg attttgactt tgttgaccca
    33301 tcttcatctt ttcttttttt cattttagca gggatttcca tagtacttct gaacggaaat
    33361 tatagaaaag taaaattgac acttttccct ttttatttta ctattatttt ggtgtgttgt
    33421 tctattacat ttacaagata taacttatgt attggaaatt tagaagcaag taaaaacatt
    33481 gataattata atttaaaact ttatttttta gaaagagcag aaaaatttta tcctttttat
    33541 aatgtttatt tatataaagg gaaagtgttt tgccagatgg gaacagaaaa tagagatgaa
    33601 gaattattaa agaaaggtga aaatttactt aaatactcta tttatttaaa cccttattta
    33661 gttaatgctt atagagaact atctttttta tatcaacaag aaggtagaat aaaagaaagt
    33721 gaaaaaatgt atttgaaact tattgaactt tatcccaata aaaaacagtt taaccttgaa
    33781 gtgagtattt tttacaaaag tttaaaaaat gaagagaaat tcaaatattt ttttgaaaaa
    33841 agcaaatacc tttttcctgt ttcagtggag gaaggtatta tttgtaacaa ttacgaaaaa
    33901 tggatagaat tgcagaagta gaaataagaa aatgcgataa acttttatat tttaaagtaa
    33961 gtaaggatgt gcaagttgaa aagggggatt ttgttattgt tgagtttacc cagggaacta
    34021 ttgactgggg aagaatttat agctttgttg aaacaaaagg gtttttccca catacaatat
    34081 ctgggaaggt tttaagaaaa attacagata gtgaccgcat aattattgag gaaaataatg
    34141 caaagaaaaa agatgttttt gatatattca ataaaaaaat aaaacaatat catttgaata
    34201 tgaaattggt agatgttgaa tattcatttg ataggacaag aataactttt tattactggg
    34261 cagatggcag aattgatttt agaaatttag taaaagacct cgcaaaaatt tttaattgta
    34321 gaattgaaat gaaacagatt gggttaaggg atgaagcaaa aataaaaggt ggttatggaa
    34381 tatgtggaca accactttgc tgctatgctt tcttaaaaaa atttgatact attacaatga
    34441 gatttgtaaa aacacaaaaa ttacctcttg atatgcataa aataacggga atttgtggta
    34501 gattgctttg ttgtttaaga tatgaagatg aattttataa aaaagaaaaa gtgggaaaaa
    34561 aatgaaattt tatataacaa ctcctcttta ttatgtaaat gctaaaccac atataggaca
    34621 tacttataca actgttgccg ctgattgtct tgcaagattt tatagaatga gtggatatga
    34681 tgtttttttc ctcacaggga ctgatgaaca tgggcaaaaa atatcaatag cggcacaaaa
    34741 cgaaggattt ttaccagaag aacttgcaga taaaatctca caaatataca aaaaaacatg
    34801 ggaaaaatta aatatttcat atacaaagtt cataagaaca actgaaaaac aacatcagga
    34861 agttgtaaaa aatgtattta ataaacttta tgaaaaaggg gatatttata aaggacaata
    34921 tcagggatat tactgtattc attgtgaaag ttatgtaata cctgaagaaa aagaaaatcc
    34981 agtatgtcct gattgtaaaa gaaatgttga aaaaatttca gaaacatctt attttttcaa
    35041 attatctgcc tattctgaaa aacttttaag agttattgaa gaaagtgaat ttataaaacc
    35101 agatttcaga aaaaaagaaa ttaccaattt tataaaagag gggttatatg acctatgcct
    35161 tacaagaaaa aatgtgaaat ggggtattcc ctgcccaatt gatgaaaatt atacaattta
    35221 tgtttggttt gatgctcttt tgaattattt aagcggagtt ggttatttat atgataacaa
    35281 cacatttgaa aaatattggc cagttgatgt tcaacttata gggaaggata taatgagatt
    35341 tcataatatt atctggcctg caattttaat gtctttacaa cttcctttac caaaaacaat
    35401 ttttgctcat ggatggtgga ctatgtcaaa agaaaaaatt tcaaaatcaa aaggaaatgt
    35461 tatatctcct gacgatttta taaatgagta tggagtagac ccatttcgtt attttattat
    35521 gagagagata ccttttggac ttgatggaga atattcagca attactttca aaaaaagata
    35581 taatagtgac cttgtaaatg actttgggaa tttacttaac agaactctta accttattga
    35641 gaaaaacttt aatggaatta ttccagattc ctcatctgaa gaagaaatta caaatttaac
    35701 agatgaggtt tttgaaaatt atagtaaaaa gatgaaagaa atatcttttt cagaaggcat
    35761 agaagaaata tggaaactaa taaattacct taataaatat ttagatataa aagcaccatg
    35821 gagacaaaca tgtgaaaaac cagaaaaagt tatttactct gttgttgaag ggataaaaaa
    35881 tgtatcattg atgctttctc ccattatacc tgaaacaata aaaagggtat ttcaaatgat
    35941 tggaatagaa aataaaataa acaaagaaaa tttttcttca ataaaagaaa ggataccatg
    36001 tggaataaaa acagggaaaa gagaagtact ttatataagg aagaaatgaa accaatttat
    36061 aaaagaattt tagttaaact ttctggggaa gcacttttag gtaaaaagaa atctggaata
    36121 agttcagatg cactagtttc aatatcagat gaaattaaag aagttaaaaa tatgggatgt
    36181 gaaattggtg ttgtagttgg aggaggaaat atttatagag gcagtcaacg agataaagaa
    36241 gtaatagagc cagttactgc tgattatatg ggtatgcttg ccacagttat aaatggactt
    36301 gctcttcaaa attgtttaga aaataaagaa ggattggaga caagagtttt aagtgctttt
    36361 gaaatgaggg aattctgtga accatatata agaagaagag caataagaca tcttgaaaaa
    36421 ggaagaatag taatttttgt aggggggact ggaaatcctt attttacaac tgatacttct
    36481 gctgccctta aagcaattga aataaaagca gatgtcatat tgaaagcaac aaaagttgat
    36541 ggtgtttata cttctgaccc tcttaaagaa ccttctgcta aaaaatttga taaattaact
    36601 tatatggaca tattacaaaa tcaacttaaa attatggata gtactgctat ttctttatgc
    36661 atggaaaatc atatacccat tgttgtgttt aatcttttca aaaagggtaa tttaaaaagg
    36721 gttgtttgtg gacagggaaa aagaacaatt ataactgatt aagttaaaac ataaagtaaa
    36781 atcccaaata tttccccctt ttataaaggg gaaaggaata attagagaca caaaagccca
    36841 gaggaagaaa aaataaaatg aacaataccc ccctcacccc ttccctctcc cctgagggga
    36901 gaggctcgcc cgaggagcca ggagtgagga gtaataagta gataacaaaa attttggcag
    36961 taccccccat aaataaggag gtatttgatg aaaaatcaga ttgtaaaaga agttgatagt
    37021 aaaatgaaag aagttgtaaa tttctttaca aaagaaatag gacttttaag agttggaagg
    37081 gcttctgttt ctttattgga aggaattatt gtagagtatt atggttctaa aatgccaata
    37141 aatcagttat caacaataac aattcctcaa ccacaactcc ttataattca accatgggat
    37201 aaaaacatag ttggtgatat ggtaaaagca attcagtcaa gtaatattgg acttaatcca
    37261 atatctgatg gtaatgtcat aagggtccct gtacctcctt taagtgaaga aagcagaaaa
    37321 gaaattgtta aaaaccttta taaaatggct gaagaggcaa aagtagaaat aagagaaatt
    37381 agaagacagg caaacattca gttaaaagaa aaacaaaaaa acggtgaaat ttcagaagat
    37441 gatatgtata atggttttga tgaaattcaa aaaatgacag ataaatatat tgaagagatt
    37501 gataaaaaaa caaaagataa agaaaaagaa ataatggaat tatgaaaaat attgtaatag
    37561 caatagatgg tccagcagga gcaggaaaaa gtacagttgc aaaattagtt gcaaggaaac
    37621 ttggttttct ttatatagat acaggagcaa tgtatagatg cctgacatta aaagcaatgg
    37681 aaaacaatat aaactggaat gataaagaga aactcataga aatggcaaaa aatacaaata
    37741 ttgaatttat ttctaatgac aattacaaag ttataatgga tggtagagat gtaagtgaag
    37801 caataagaga tgaagaagta agtaaaaaca caaaacatat tgcatctatt ttagaaataa
    37861 gagaaatttt atgggaaaaa cagagaaatt atagagaaaa atttaacatt gtcatggaag
    37921 gaagagacat aggaagtaag gtttttccag atgcacagat aaaaatttat ttagacgcct
    37981 caattgatga aagagcaaaa agaaggtatt tacaacttaa acaaatggga attgaggaag
    38041 aaatagaaaa aataaaaaaa gatgttgaac aaagagatga agtagataga aaaagggaaa
    38101 tagcccctct tatgaaagtt cctgaggcaa tttatattga tacaacaggt cttacaatcc
    38161 agcaggtagt tgatacaata tgtgaccttg ttcagccgtt atacttggga agataatttt
    38221 aattttgtat tattttctcc accatttata agataaagca aaggcaaagg tctgtccact
    38281 ccttcaataa actctggtct tgcttgccat ggacacacag aaagaacatt tgtaaaatcc
    38341 atccaatatt ctaatcttga aacttttaaa tcccatatca tatgaaaatg atttgctggt
    38401 gcatattgtt tgtattcaag cattgaagca tactttggga caacaaatgt atgtggccaa
    38461 gtataattgg atgttttaca aacttcttct gcaagttttt caggtaattg tcctgtttct
    38521 gcctcatccc atataagaga aaatatattg ttaattgatg aataagcaag tcgcccggca
    38581 attcctttta taccaccagg ggaaacaaat tttactgaat ttccaccacc agggaaagaa
    38641 aattcctcag caataggaaa agtaattctt ttcataattt cttccatttt ctcaccttgt
    38701 tttcctgccc agttaaatga agcacttcct gaattagtcc catcaacaaa gccctttttg
    38761 aaccacattt catctgtctt tatatttata ccaatttttt ctgcaagttg ttttatttcc
    38821 catggttccc agacttttct gaaatccata aataaaggcg gctctcctcc tgttaaataa
    38881 gtaaaaaata acatcgtaag taatccctga acatctgctt ccgttgcata aggaattact
    38941 cttttcttcc ctgtatggtc aaatgttgaa ttaaaaagtg cctccataac atctgcaaca
    39001 ggaagaggaa tacctcttaa atcagaaccc cattcaagtt gactcataaa accgcctcca
    39061 acagcattta agtccttcat tatatctctt acaatcaaat acattgcaag tgattgattg
    39121 aacttttcgc tatcttcttg gtttcttatt tcaattcctc ctgaatattt ttctagccag
    39181 tttcttaatt cagttagttc atttttgtca tacacctttt ttgaaagcat atctgaaagt
    39241 aatttcatat caagacgggt aatttctatc ccaaatattt ttcttgtagg aataatatga
    39301 gcaagtgctg tctccattcc catagaatca tgcccaaata taacaactct tctgcctttt
    39361 agtgctttat atgtaattgc tccataacac caatcaacta aatcttgtat tgttttatca
    39421 cttattatcg gttctaaacc agtatctgac caatttccaa tatttatatg cataagttta
    39481 ccatattggg caactgctcc attacatgcc tgaacataaa caacacctgg ttttggtcca
    39541 ctatttccac atgtcaaatt tattggtgta tcctgaggaa attgctgtaa aaatgatata
    39601 agcgtaagtt gaggaaatgc ccatgtatcg ggaactccaa taaaaatatc tactttttct
    39661 tctttaaatt gttttgccac aatatcacct tgtttctctc catcaattaa aacatcacta
    39721 taaacaactt ttacatcttc tccaaaaaat tttattctat cagcaataat atcagcagtc
    39781 attttaatta tattttttac tctctgtctt gattgttcat caattcttgg gtcagatgga
    39841 gcaaaaacac ctatgacagg tgtttgacaa gaatttttta cttcccctac tttaattgcc
    39901 tttcccatat tttccctccc gattttttat atttttataa aactttactt atttcaaatt
    39961 tacaatattc ttttttccct gtcaattttt ttggcaggtt gtaaatcttt atttattagc
    40021 aatttagtaa actttaaagg aagagtaata gaagcagcat aaaaacagcc cctatatttt
    40081 cccaattcta aattctgcca ctcataaggt gctcctttat ttctcttttc cttcgttatt
    40141 taacacgatt aaaaagttct tgatatgttt tatatataat ttcttctgca ttttcatctg
    40201 gtaaagcaaa ttctttttgt acattcatta taccggattc attgtctctt ttatgctcat
    40261 gagatggcat taggtaagaa agttcatcat tacaaagaga attcacccat gtgtacttaa
    40321 aaggacttgc ttttcgcagg ttcatcgcaa tctcgtggaa tacttcacca catagtccta
    40381 atattacaag ttctcccaga catataattt gtagttccga aaacactcct ttttcatcct
    40441 gttttccacc agcaactctt cgtccttgaa gatatgcctg atgtcgtaaa gttaaagttt
    40501 tttgtaaggg gaaatacacc ttcttgcttg cagaaataac aggtgtaggt tcaaaaggtt
    40561 ttaatttttc ccatatctct tttgcttttt cataaagttt ctgaccagca actggaacaa
    40621 atttctccgc ttctgcggcc ggtacatcat gttcaaaaaa tgctgctgga tgaacattac
    40681 cttctggtcc tgccattaaa gaaaaaacaa atttattgcc tatttctttt tcaagttttt
    40741 ctgctgccca tccaaaataa tcactattga gagtagtgct tcccatagaa agtgcattat
    40801 gacatccaaa atttcccaac ccacaaatag gtcttccttc tgtatcttca aataatacaa
    40861 gtcgcaatga gtcatcataa ggtgatatag attcaatctc ttcttctttc acatgctcta
    40921 tggaatatgc atgagttaaa cttgcaacac ttccatcttt catcctgatt cttggagttc
    40981 caataccagg aacagagatt tcacctattc ttccttttgc agttcttcgc cgactatctg
    41041 caagaaatac agaactggca gcatatcggt aaagcatatc taaatatgcc tgtgtttcat
    41101 tacttagttt atcatatctt attaaaacac caccagtatc gtacgggtca tcataatcaa
    41161 tcacttttgc tgaggagtga gtatgagtgg cacataccat aatattttct tgtggtatat
    41221 ctgtccactt acttgcaagt tctctaatac ctcttgtaat attcggaaaa aattcactta
    41281 tatctaaaag cactattgct accttatttt ccccatcatc aaaaacagtt gctcttgcac
    41341 atagttctcc tattacatca gtagccagtc ctttgggccc aattcgtctt gcctggtcaa
    41401 gaggtggcat aatactatat tttgctgctc caactttaat tttctccctg ctcatatttt
    41461 ttcctattta taaggaaaac aatttttttc tttttcctat gtaaataccg aaaaacaaaa
    41521 aataattaac tttttttaac ttcttgaacc atagaaaccc atttttctac acctttttct
    41581 tttatatcat caaaaacctt tttaattgca agtccaacaa gttcagcagt tttttcatta
    41641 ttgggttgtc ttaaaggata tgtcatacca aaatgtcttc tacaatgctt ttgtgcttct
    41701 tgatattctt ctggattata agaaacattt ctgctgtggg gacaaatcca tgggcatcct
    41761 tttccatatg cattttttgc ttgaaataca gtcatttcag gaagtataaa actctgccat
    41821 attccgactg gaactccttc ctgtcttatt gcttccataa ttgaatccct taaaattact
    41881 tcatcacctg aaaaatcaag tttttctggt tcaattctta atgtatagtt ataccatata
    41941 tgctcgcatc cctctggagt ataaggtatt tttaatcctt taatatcttt tataatatct
    42001 gtcagaacag aagcattttt tttctgggtt tctaaatatc catcaagttt tgtaagttgc
    42061 gcccttccaa aagcagcagt caggtcatta tttctataca tccagccaag agcatatgca
    42121 tgatagtccc tactttctga tggtcttctt gtttctccaa atgaccataa catttttgct
    42181 ttttcatata tttcctcatt atcggtcaca aacataccac cttctcctga acataaacat
    42241 ttattttgat tgaaactgaa agcaccacaa tccccaattg tccccacttt tttgccctta
    42301 tattttgccc catgtgactg acaggcatct tcaataactt ttaaattata tttctttgct
    42361 atttccataa ccttatccat ctcaacagaa agtccatgaa gatgaactac aataatacct
    42421 tttgtttttt ctgttatctt ttcttcaatt ttatcaacat ccatattcat cgtttcatag
    42481 tcaatatcaa caaaaacagg aatacaatta tgatgaagaa tacaggtggc agaagaagac
    42541 catgtataag atggaacaat tatttcatct ccaactccta aaccacaacc tgctatggac
    42601 atatgtaatg ccgctgttcc actatttgtg gttattgcat atttgttccc attccaggca
    42661 gcaaattctt tttctaattc aacacaattt ggtccataag catgatttgt tccttctaat
    42721 gaatttaaaa catattttct atctatttca tctattggtg gccagtcctt tatttctttt
    42781 actgttttct ctcctccaaa aatagcaatt ttcatttttc ttcctccttt tgtaaatgga
    42841 agttttacca aaatttttgt tatctgttaa tttccccctc actctcgccc tctccccata
    42901 ggggagaggg tatgggtaag gggcgatttc atcttttttt catctatttt ttgacactac
    42961 caaaaaggtt atttatttct tttaatgtct cttcaacaat tacctgccct ccctctggtc
    43021 caactaaatt gtctacaatt tctgcaccat aacttctccc ttttactgct ctttctgttg
    43081 gtaagtaagt acctccacca tttgatagtt gaataagaaa cgtttgaatt gatttactcc
    43141 ttgcttttat ccttatacca taatcaagaa aaagttcaaa cctatttgtt gcaaaagcaa
    43201 tatctccaag acgaattaca tgtaattcca taggtaaata tgggttctcc ttttgcttat
    43261 tatatctctc aattacttct ttatttctac ttaaatgtct aaatgtattt ggcgtttctt
    43321 tttctctctg taatttctca atctcttgtt ttgcttgttt tacttcttct tctgtcacca
    43381 tcctcatagg taaatttatt gttttaacta tgtgtttaaa ttctacctta tcttttatat
    43441 cttcctttgt gtatggtaaa gcatcatcaa ctgcatttgc tattcgtctc cctatctctt
    43501 tccttgctga catccttata tcttcttctt tctccttaaa tccccttaat tccaacatcc
    43561 taccttcttc tttcttacca ataagtaagt gtggtgattg gtctcctgct ggtgaacatt
    43621 gtggaagtat aaatatatct tttgaatacc ttttgtaaat ctcttctctt gtatcctgcc
    43681 agaaatctgc tgataagaaa agagcatttt ctgtttcctg tgatggacag gcaatattta
    43741 ttatcatacc tgttaatttc ttgttcttat cccaggtaaa aagtaaattt acactatggt
    43801 cttcatatcc ttctatatga ctgaaattta ggtcatcagt attgccatac atctttgatg
    43861 aaccgtcaaa ataggtagct cttctattat gaccaactac tgcatgccca agtccataag
    43921 atatcatccc ttcttttctg ttctgccatg actttataat acaatcaccc gcttttttca
    43981 caaaaaactt tgtgtattca gaaggactca ttactccttt ttctgatgtc tcatatagcc
    44041 cttctttcat tgtaggtcct gtatgagtat gagttgctcc aaaagatatt ttctgaacat
    44101 caatctctga cattttaccc ttgacatatt ctcttacttc attacatacc tcttcgtaaa
    44161 tataaacaag gtctgctgat aaaagaattg cctgctcccc tttttctgat tcaatcgcta
    44221 aagatgttgc cgttacaggg tcatttacat attttgaaac ccttgcataa tgctgaccat
    44281 gtaaaagaac tggcctatct ggtgttatat cttcttttgc ccatccaatt aaaattttct
    44341 ctctttccat tttttctcct tattttatgt tttttagaaa gttcaatgct tttattattg
    44401 cttcttctct gtcatattta tacccttcat attcaatact tacataagac agataattct
    44461 ctttttccat tgtcttaaca agttttttat agtcaattat cccttctcct ataagtgctg
    44521 gtatgtactt cttcccatct aacccctctt ttccatcatt agaaatttcc cagtccttaa
    44581 aatgtgtatg aattacatat tccttcattt taagaaaact ttctgatggc tcttctccac
    44641 ccaatataac atttcctgaa tcaaatgtca cttttacttg tggtatttct tttattattt
    44701 ttttcatatc ctctgaattc aaaaaaggac ctcttatatc tggaaattgc tcaacagtta
    44761 ttgaaatacc ataattatct gcaagaccaa taacttcttc gagccctttt ataacatttt
    44821 ctctactttc atctcttgaa tattcatcct ttccaccaat tggaagcata attttatctg
    44881 ttgctaaaac cttactttcc tcaatccctc ttttaatttg ttcaatccca tgtcttctta
    44941 aattttcatc tttaaaattt atatctacaa aaaatgtata gcaaactgtt ttcaatccat
    45001 aatcatccat aatttctttt acttcgtctg cccgccttcc atatagtcca acccattcta
    45061 taccatcaag tcctaaattt cttgtcaatt tacaaagata ttcaatatcg ggattttctc
    45121 cccattttcc ccctgccatt gtataactca tcattgagta tttcatttct cctccattaa
    45181 agttattaac atcaaaaaac aataaccccc aattccctct atttttttgt tctatgatat
    45241 ttatattatt tttacatatt ctacataaaa agtataataa aattctcctg ggctggtgta
    45301 gtccttattt gaaagtatat cccacagagg aaaaagaaga tatgatacaa gaagtatttt
    45361 cctttcgtac attttttgac cacccgatgt tcatataaaa aatttataat tatcctattt
    45421 ccctgtcaat ttttcattat ttattttcac cttataaaga tcaaaaaata acatttaaga
    45481 aag
//
LOCUS       DAOOVW010000009        41062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_611_length_41062_cov_7.305728, whole genome shotgun sequence.
ACCESSION   DAOOVW010000009 DAOOVW010000000
VERSION     DAOOVW010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 41062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41062
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_611_length_41062_cov_7.305728"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            345..3278
                     /locus_tag="PLW95_02245"
     CDS             345..3278
                     /locus_tag="PLW95_02245"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOV21486.1"
                     /translation="MEKLQLWEDEVKKLQNNIEAQMSLTHWKFKQGIVKNGESLELDD
                     TDWEDKTTTTWSLKEGPAYFRTYLTVPKKIEGIDIENSDIDITFCFPSGVEMFIDGKK
                     VYTHKFWADKIGTPIPLIQKAKAGDKMLIVFKTPAGDGLGYFWAKISISKIETTLFEI
                     NSILYQLKFALALAGEKKSLKKYVEKAINIINLDDITQRHWENVLCDIKEAEKILEIF
                     RNDAKKFCIHLIGHAHIDINWLWTYEDTINTCLRDFETVTKLMEKYPDLTFSQSQAHI
                     YKIVEEHNNELFNKVKSRIKEGRWELTASSWVESDLNMIDGESIIRHILYSTKYSLEK
                     LNTKTKIFWSPDTFGHPITIPSILASAGMKYYYFMRCGKGFPLFRWKGIDGSEVISFN
                     SIYNNYILPEKIMPNFIEYLNKYKLHNFLFVYGIGDHGGGPTEKDIKRKNMMEEKPVI
                     PTLVFSTAEKYFNEIEKYRNKIPICKGELNTVYEGCYTTHSDIKKNNRECETLMHTIE
                     TLSAITGIDNEKEKKIEEMWQSILFNQFHDILDGSAIHSSYEYSSKLAEGVKKNGEIL
                     IKEMMDNLSYKKSKDKIIVFNPLGWKRRFIIPKEIIIDEIDGYGYKTILSKNKMQCQS
                     KVIKKINNDYETEFYRIKIDHTTGLIRTLYDKKNKCNVLVPLYPKSDDISCWLAEKAG
                     NLISVNWEQPHSMSAWNIGNIYRQDYLLDAESIEIEEYPLYTVIKIKRKYMESEILQK
                     IILYSQLPFIDFENEIEWKQEGNNKDGVPMLRVSFNVNMEHPEAYFEVPFGAIKRQIT
                     YQEYPALRWAALKDKNYWVTLFNKDKHGYYINGNNLSLTLLRNPYEPDTKPDSGFHHI
                     CYRLQFGKLNALEITKLAMEYNTPIITISGETKSEEFYPFLIRGNILPTSFKKAIGRE
                     SYILRMVEMFGKEQDIEINFTRPVRKIYISDAIENRIKQIKCSKYNIKITVHPFQILT
                     LDIEL"
     gene            complement(3421..4977)
                     /gene="yidC"
                     /locus_tag="PLW95_02250"
     CDS             complement(3421..4977)
                     /gene="yidC"
                     /locus_tag="PLW95_02250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOV21487.1"
                     /translation="MDKMKDELRLIIAFVLSILIIIFFGKFTSQKQPVKTQQQIIENT
                     TNEEKTSENEKITETKLPSEIGKIEKKLSAYENNNFIIKINENGGFIDLIGLKKYHRD
                     DEKYFPIIENNYLFLTFQQGKLEAIEKLWNIEEKENNITLWTNIDKLYIEKDITIPTN
                     SHTFEVEIKIKNNSNEKQEVDDLIINLLSLNKNEIKKSQNYNRYLRTELLIGSNNNII
                     KRNITKVKGKETFENCNFIALQSEYALLLYRTEKPMDAFISNEENIFTAGFIVNKLPI
                     EKNEEKILKFNFYAGPYDYFIASKEVKENIFGNGFFVLMGRFLFAALYNIHKIIPNWG
                     WAIILLTLIIKIVFFPLTRNSLRSMKQIQKLRPYLQDLQKKYKNDPQKMQKEMFNLYR
                     EYKINPFGGCIPMLIQFPIFIGFFIALRNSIFLRGAPFMLWIKDLSVPDTILRVGHFP
                     LNILPVLMTLTAFWQQKLTPSDPSQKALTIMMPLMFLFLFYNFSSGLLLYWVTMNIMG
                     IVEQYLIRTK"
     gene            complement(4965..5183)
                     /gene="yidD"
                     /locus_tag="PLW95_02255"
     CDS             complement(4965..5183)
                     /gene="yidD"
                     /locus_tag="PLW95_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006805375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOV21488.1"
                     /translation="MRKILIFFIRIYQTIISPFLGRNCRFYPNCSEFTIQAIEKCGWK
                     GILIGIKRILRCNAFNEGGYDPIERWIK"
     gene            complement(5168..5467)
                     /gene="rnpA"
                     /locus_tag="PLW95_02260"
     CDS             complement(5168..5467)
                     /gene="rnpA"
                     /locus_tag="PLW95_02260"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOV21489.1"
                     /translation="MRKRIYKIIKEGKKVQGVFFDLYFKGSDDYKIGFIFSGKIGKST
                     KRNYVKRVIREYWRKNLKGGDFLFVLRHEVVNNKSKEEIKKELQKMVEIAKCEKF"
     gene            complement(5467..5601)
                     /gene="rpmH"
                     /locus_tag="PLW95_02265"
     CDS             complement(5467..5601)
                     /gene="rpmH"
                     /locus_tag="PLW95_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011715297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOV21490.1"
                     /translation="MSITYKPHKLRKKRKMGFRAKMQTKNGRKVISRRRKKGRKILTP
                     "
     gene            complement(5668..5856)
                     /locus_tag="PLW95_02270"
     CDS             complement(5668..5856)
                     /locus_tag="PLW95_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21491.1"
                     /translation="MLLLKVSLFCSKIYNTQSTTMPESIKTRKAKFLHPLLKDRIVPS
                     FNKGRVGSPVGLRPRDGF"
     gene            complement(5864..6619)
                     /gene="surE"
                     /locus_tag="PLW95_02275"
     CDS             complement(5864..6619)
                     /gene="surE"
                     /locus_tag="PLW95_02275"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOV21492.1"
                     /translation="MKVLITNDDGIYSKGLKILVECLPSSIEKIIVVPEFETSAISHA
                     INLFNPIRIKKIDLFYSNCYIVNGTPVDCVKIGVKEILKNRPDVIISGVNLGPNLGMD
                     ILYSGTVAAAAEGAILGIPSIAISITDYKNFDFLSIKKVLKKIIKSLIKIKLPEDTIF
                     NINIPNLPLETIKGIKFTFQSKSRFIEEYEKRIDPKGVPYFWLKGNFKIKGEKGSDVD
                     AIKSGFISITPLNLILTDDKFLKELKKLYKNFT"
     gene            complement(6654..6725)
                     /locus_tag="PLW95_02280"
     tRNA            complement(6654..6725)
                     /locus_tag="PLW95_02280"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6691..6693),aa:Ala,seq:cgc)
     gene            6861..7973
                     /locus_tag="PLW95_02285"
     CDS             6861..7973
                     /locus_tag="PLW95_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOV21493.1"
                     /translation="MKKLIAGIIGAGGICDYHILGYLSTDVEVIAIADPDIKRAEERG
                     KKFGIKYVFSSYQEMIEKLPEISLISVCVPNKFHSEISIYCLEKGKNVFCEKPPALNS
                     KETEKMKIAAEKNMKILMFDFNNRARPESQALMKYIKNGDIGKINSAQALWIRRCGIP
                     GFGGWFTQKKLSGGGPVIDLLHMIDLALYFMEFPEPDWVLSQTFYDFSGNPDFKGPWG
                     IPDVEGGIMDVETSAHSFIKFKTGQVLFARNSWAEMNKREEVSVIFQGTKAGGMIRRL
                     FGRDGIDETAIDDCELYTMENGKPVNKKIIVQPDEKMGRERAVINFVNVVRGIEEPYS
                     TLEQAVILMKIIDAIYKSSETGQPVKIATTQSTSVD"
     gene            8285..9868
                     /locus_tag="PLW95_02290"
     CDS             8285..9868
                     /locus_tag="PLW95_02290"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOV21494.1"
                     /translation="MKKGYFFIIVGFLFILCGCSVKYYEKSAEKDIKEIWDKSYKKVE
                     EKKLPDSILQEDVFPETRVLDLSSAIKIGLENNRNYKSRKENVYIKILDYTYQKYLLK
                     ERYGIGGDISSEKSGDETIGADLNFNLIKLLSNGAKISFDITQDFLKYFTGNKEKDFQ
                     TFLSLNFFQPILRGAGKDIALEGLTQAERNVIYEIRDFLRYEKQLSTDIAKKYLSLVA
                     QKKNIETYRRNYEFLKKIRERNENLAQAGRLSPMQVDMANQDEYSASQRWITASNNYK
                     NAVDSFKIYLGIQPTEKIEIEDKQVEDVLSKGIQKFDIDLTSGTEVALKNRLDLITFY
                     DMVADAERQVKIAINNLKGTLNLNMSIESGTEKKSRPTFDLTKPEYSAGLEFELPIDK
                     LPERNAYKKSLISLDRAKRNFEEKKDNVKLEVIENYRNLEEAYQSHLIQRNSLNLAEK
                     RVDSTDLLFQAGRAETRDLLEAQNAYLSAETSLTSASINYISAYLDYLLSCEMIEVDI
                     EKVWKGDKYEEIFGKIDKK"
     gene            9834..11588
                     /locus_tag="PLW95_02295"
     CDS             9834..11588
                     /locus_tag="PLW95_02295"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOV21495.1"
                     /translation="MKKFLVKLIKNKFFILGILVIIIIFSVVRYLKIKQAKSQLLTYT
                     VKKQDLIISVIENGNVTALKSLKIINRVPGTRNILGVVDEGTQITEEDVKNKKILLKL
                     DSNDLEDRLDTLEIDVENSLAAYTQALQELEITKKQNESDITKAELNVKFAKMDLEKY
                     LGNKISEAVLKEKEVDYKKIINSPDLGGEAWNTKKDLENTIDIAKEELERAKNNLEWS
                     VKLAEKGYITKKDLEADQLSCKQRQANLEMAELKYALFINYDFPKKVEQLLSDYKENI
                     NELERVKDKSKAKLIQSESNMRSKKATYLLRKNTFEEVQEQINQCTIYATQPGFVVYA
                     TSSRPWRTEGPIQPGTSVRQFQELLNIPDFNYLGVDVQVHETNIKRIKEGQKAIITID
                     AFPDTKFEGTVKKISLMPDAVMKSLNPDINVYNTQVVFDKSASFLKPGMSAQVEIIVD
                     KKAKVIAIPVVSVLFEKNLPFCYVLKGNKIEERKLELGDSNDRMVEVKSGLKEGEVIV
                     MMPETLGPEVKKEEMYEKGKITTESKEKQVIENVQEIESKQSTEIEQGIQGGQNIEGK
                     QGVKRGQRRERGERKVNE"
     gene            11581..12267
                     /locus_tag="PLW95_02300"
     CDS             11581..12267
                     /locus_tag="PLW95_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV21496.1"
                     /translation="MSENNNVIELLNIKKIYKMGTIELEVLRGISLSVKKGEHISIMG
                     PSGSGKTTLLNILGCLDKPTYGQYFFQDKDISKLDDNSLSEIRSKYIGFIFQQFNLIP
                     QLNVIENISLPLFYQGIDENLMMEKAKDFADMVGLSDRLYHRPSELSGGQQQRVAIAR
                     ALANDPLIILADEPTGNLDTTTGKEIMKLLCRLNEEGKTLLVITHDINIANYSKRIIK
                     LLDGRIVNES"
     gene            12257..13543
                     /locus_tag="PLW95_02305"
     CDS             12257..13543
                     /locus_tag="PLW95_02305"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOV21497.1"
                     /translation="MKVKIYHILKILKLGLKNIFLHKLRSFLTILGIVFGVASVIAML
                     SIGEGASYEAREKIKQLGSNNIIVRSFNPPSSIKTSQDAAAIGLYGLKFDDLEEIKTI
                     PSIKNVVPTWECKEDISSENGQITGRIVATEPSFLKVMNLELIYGRFFTDIELKNSRN
                     FAIIGYSIKKNLFPLENPVGKTIKVRSYYFKVIGVVKEKAVNLTGTSFESEDINFDVY
                     IPFTTGRVYFGKYEIANKRGPVFNWEKNLVEYHRFIINLLDTSKILTVASYVEKILLS
                     NHKSSDYEILIPLQLLQQAEHTKKIFNIVLGSIAAISLIVGGIGIMNIMLATVTERTR
                     EIGIRRAIGAKKRDIINQFISESVILSGTGGIIGVLLGVIIPKLVTKFSGMTTIITFW
                     SIILSFTISVAVGITFGIYPARKAAELDPIQALRYE"
     gene            13594..14088
                     /gene="lepB"
                     /locus_tag="PLW95_02310"
     CDS             13594..14088
                     /gene="lepB"
                     /locus_tag="PLW95_02310"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOV21498.1"
                     /translation="MKIYIRILIDIILVVFLFLVGYFFIYKEMHFVKIISSSMEPTIC
                     VGDRVITVKKGVVQRGDIVVIEAPKGKKELLTKRVIGLPSEILEIKDGSVFINRKKLE
                     EPYIKEHPRYELKVKIPEKKYFLLGDNRNKSEDSSIWGPVDEENIKGKVICRYWPLKN
                     FSIF"
     gene            complement(14085..14951)
                     /locus_tag="PLW95_02315"
     CDS             complement(14085..14951)
                     /locus_tag="PLW95_02315"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOV21499.1"
                     /translation="MFTGIDFLTIILLLIGIFIGIIRGFVGNLIDIIALCIGISLSSI
                     IYRGPVTLFRNFGITGNAIELTCFLLSVLILTFAIIILFELLRKRIDIKHIVDRIFGV
                     LTGFIIGMVLTGIIYITMSSSYDSAKNLQESKFPKYIIKYIPKVYEKTDKIGIILPKM
                     FFISDSYEEEFNYESKKIKFEKLNFVKFENLTCIKCNSKVEFDGYIPYVGAALISKFT
                     CTSCGRISDGCQTFEIFHFLYHQCPIDKAITRMKFDCPRFPNHEWIAPRGPCPVDKKE
                     LPLWDWTPPEKY"
     gene            complement(15030..15431)
                     /locus_tag="PLW95_02320"
     CDS             complement(15030..15431)
                     /locus_tag="PLW95_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21500.1"
                     /translation="MKKLFIVLTGIFFLLSTPAYLKAEEKPKKVPREEIAGNPPRPMP
                     NENLRQAMGIRIKMRKIEEEAIKNDPELQQLQNQITELHKQLRTKLEQKLANDTEYKK
                     LQEKLNTIKEQQEEKRQKPEEIKKKRKKAEN"
     gene            15503..16159
                     /locus_tag="PLW95_02325"
     CDS             15503..16159
                     /locus_tag="PLW95_02325"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HOV21501.1"
                     /translation="MKQISIFDLDGTLIDAYSLIHISLNFTRRKLGYNELDYHLVKKS
                     VGGGDIGLIKKFFKEEDRDNALKTYRETQKQYINKENVKLQKGAKEILLLLKEKKIKI
                     TIATNRNKFAANLVLEKLNITNYFDLVFTADDVKEKKPSPEIIEKIYEKFNCSKEECF
                     YVGDMDIDLKTGLNAGVDTYIVLTGSSTLDEILKVEKEANVFSDLIELKNFLLNNHLI
                     "
     gene            16525..17790
                     /gene="eno"
                     /locus_tag="PLW95_02330"
     CDS             16525..17790
                     /gene="eno"
                     /locus_tag="PLW95_02330"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOV21502.1"
                     /translation="MEIVKIHGRQILDSRGNPTIEVEVVLEDGTVGRAAVPSGASTGE
                     REALELRDGGKEFLGKGVSKAVKNVNEVIAPKIEGMCVFHQREIDKTLIELDGTENKS
                     KLGANAILGVSLAVAKASAECLGVPLFFYLGGVVANTMPVPLCNVINGGMHADNNLDI
                     QEFMISPIGADSFSDGYRMVAETFQYLKKILKEKNYSTGVGDEGGFAPDLDKNEEGIE
                     LILKSIEKGGYKPGEDIYIALDVAASSLYENGKYNFEGKKVDSEYLISFYEKIISKYP
                     IYSIEDGLAENDWDGWAILTEKLGSKIQLVGDDIFVTNPKIFKKGIEKKIGNAILIKV
                     NQIGTLTETLQTIEMAKRNGYKTIISHRSGETEDSFIADLAVGTNAGQIKSGSLSRSE
                     RLSKYNQLLRIEEILGNGSEYYGKHLIKK"
     gene            17787..18050
                     /locus_tag="PLW95_02335"
     CDS             17787..18050
                     /locus_tag="PLW95_02335"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOV21503.1"
                     /translation="MKKRRKIYLILILITFVILSIPRITLLLSSIKKLHYYQDEIVKL
                     ETENKILKEKIEKIENDPYYIEKLLRENYGLIKEGEIVYRIKE"
     gene            18053..19315
                     /gene="serS"
                     /locus_tag="PLW95_02340"
     CDS             18053..19315
                     /gene="serS"
                     /locus_tag="PLW95_02340"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOV21504.1"
                     /translation="MYSLKFIRENTDILKKAAEAKREKVNWEKFEELDNERKRIISFV
                     EERKRWQNDITEKISTRKDDKNSIEPLINQARKYSDEIRESEKKLRDIEEALNKIVAT
                     IPNIPHVSVPEGKGEKDNVVIKQWGDIKEKEVLPHWEIGEKLNILDFQRGAKITGSFF
                     PVFKNEGALLVRGMINFMIDVHKKSGFKEVWVPVLVNRDSLFNTGQLPKLEEDMYYIK
                     SDDLFLIPTAEVPVTNLHKNETIPEDQLPKYYVSYTPCFRREAGAYGRETKGLIRVHQ
                     FDKVELVKFVKPENSYEELETLLAEAEKIVQLLGLPYRVIKLCTGELSFSASKCYDIE
                     IWAPGIKKYLEVSSCSNFEGFQARRGNIKYKRRDGKTDYVHTLNGSGIAFPRTIIGIL
                     ENYQTEKGEVIIPEVLRPYVGINKIPCG"
     gene            19339..20496
                     /locus_tag="PLW95_02345"
     CDS             19339..20496
                     /locus_tag="PLW95_02345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOV21505.1"
                     /translation="MEDLKNKVKEIEKDIISWYRYFHQYPEIGFQEFRTSEKIQSLLK
                     KFKIPYQVKAKTGVVGYLKGKGKKTIGIRSDIDALPVEEKTSLPYKSKNKGYMHACGH
                     DGHIAILLGIAKVLSEMEDDLGINFKFIFQPSEEAPPSGAKQMIEEGVIDDVNLIIGY
                     HLFSFLPFKKLWIGKGPVMANADSFKIKINGKGGHGSRPHLTNDPITCAGYLITSLQT
                     IVSRKIDPVVPCVVSFGKISGGFVFNVIPDEVEIIGTVRTLSEDIRDKIKDEIEKITE
                     KICSLFGCKYEVEYNKHSPVNVNDESLSEKIIKITQKYYPESLCEFHPIMGGEDFAFY
                     SQKIPSCYMFIGMGEKCGQNHNSSFKIDERILPFAVEYLTTILINLNNPCC"
     gene            complement(20476..22248)
                     /locus_tag="PLW95_02350"
     CDS             complement(20476..22248)
                     /locus_tag="PLW95_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV21506.1"
                     /translation="MPNGFHHHGYRKYEKVEMKDAKKIIKLFIRYIKPYKFLFLLVFF
                     LVLLTSSVEIISPYLIGKAIDSYIMPKNFNGFLNFIIICLTIYGLNSAISFFHGFFML
                     KTSQSIVYSIRKDLFSKIQTIPLEVIDTRPHGDIVSRITNDVDSISNILSNSIIHILM
                     SLFIFCGIFIVMLRINVILTLVILIFIPFTLFTTRIIGRKARNYFYQNQILLGKINSV
                     IEEDIPGLEVIKAFNREEKEIEKFSDFNNDFKKIGIKAQIYAGIVGPFMNFINNINLV
                     IIGGIGGYFAIKNLITIGSIAVFITYARQFSRPLNSLAEEFNQIQIALASGTRVKEIL
                     ETEEEIEDGEIELLNVKGEIEFKNVYFSYVKDILVLKNVNFHINPGEVAALVGPTGAG
                     KTTIVNLLARFYEVDKGQILLDGIDIRKIKKSCLRNMLAVVLQDAYIFSETIKENIRY
                     GKPTANDEEIENAAKISNAEKFILSLPSGYETIISEERSLLSQGQRQLLAIARTILSE
                     PKILILDEAMSNVDPTTEKYVQRAITNLIKGKTCIIIAHRLSTIKNADIIIVVENGQV
                     VEQGTHKELIEKKGYYYSLTAGVI"
     gene            complement(22241..23962)
                     /locus_tag="PLW95_02355"
     CDS             complement(22241..23962)
                     /locus_tag="PLW95_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV21507.1"
                     /translation="MKKLTKFLKPYKKFVILAPLFMILEVVCDLYQPTLLAKLVDEGV
                     IKGNVDLILKTSILMFFIALIGLVGGILCTIYASFASQNFGCDLREELFKKIAYSPIH
                     SLNKLPTSSLITRLTNDVSQLQQLIMMLLRIVIRAPLLFFGGLVMAIYLNPKLSLIFL
                     FLIPATMLIYFKVIRKSFPLFRKVQSKIDELNRVIIENLSGIRLVRIFMRKDYEEGRF
                     DRTNNELVETIRQSFFYSLAAGPLFMLILNYGIVGVLWFGGILVRSNAMQVGEIIAYI
                     NYLMRILRSLLMIANILTFISRASASAERVNEILEIKEKEENIKNGNDLKRGDIIFKN
                     VLFSYNGKEVLKDISFHIKNEETIGITGTTSSGKSTIINLLLKFYSPKKGEILIDGVN
                     IQEIPSNKIREKISVVFQNVSLFSGTIKENILWGNEKVDEKELIKVSEICRVDEFVRN
                     FPDGYETFIGARGVNLSGGQKQRITIARAVIKKPLILILDGATSAIDMINEQKILQGI
                     KEYLSKTTFIIISQRISSLIKSDRIFVIDEGKIINQGNHTQLLKMSQFYREMAEIQIG
                     REEIKNA"
     gene            24279..25208
                     /locus_tag="PLW95_02360"
     CDS             24279..25208
                     /locus_tag="PLW95_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOV21508.1"
                     /translation="MKVLVTGGAGFIGSHTVDKLIEEGNNVFIIDNLSTGNKQNINKK
                     AKFFNIDIRDKKISDIFKEERFDAIFHFAAQINVRKSVEEPFFDIDVNINGTLNIITN
                     FLKYGKGRKFIFSSTGGAIYGDTKTIPTSEKIIPFPLCPYGISKLGVEKFLHYYSYFN
                     NLEYVALRYGNVYGPRQNPYSEAGVIAIFIGKILKEEICTIYGDGNQTRDFVFIEDVV
                     KANLMCFKKNVKGVFNVGTNKETSVNKIFNCLKKISGKNIKAIYKDEKTGDIKRSCLK
                     IDKIIKKVGWEPDIMIEKGIEKTYKWFKGNFEQ"
     gene            25205..26191
                     /locus_tag="PLW95_02365"
     CDS             25205..26191
                     /locus_tag="PLW95_02365"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOV21509.1"
                     /translation="MKYLITGGAGFIGSHIVEELLKRNSEIFVLDDLSTGSLLNVKDF
                     LRNENFHIFIDTILNEHITEELVRRSDFVFHLASVVGVKRVMENPIDSLIINILGTHN
                     VLKSCVKYNKKVLITSTSEIYGKNKNVPFNEETDIVIGSTKKKRWSYACSKAIDEFLA
                     FAYNEEQNLSVIIVRLFNTVGSRQTDKYGMVIPKFVKQALKNEVITVFGDGNQTRCFI
                     SVKDVVETLLKLIEKKEAYGDVFNVGSDEEVKIKDLSFKIKEITGSKSEIKFISPEKI
                     YTSGFEDMERRVPDTSKIKKIIDFKIKYSLEDIIKDVVNYYKNIQATVLPEK"
     gene            26459..27982
                     /locus_tag="PLW95_02370"
     CDS             26459..27982
                     /locus_tag="PLW95_02370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOV21510.1"
                     /translation="MNRKKENTLLFLILFSGFIIRLVYLYFFKDTAFFNPYLMDKHDQ
                     KTFILWAETILKHPFYVDGKVFYMAPFYPYLLSLFYLLSSGNILIILIFQAVIDTGTC
                     YLLYLIGKKLFGEKVGLISSFLGCFYKTFIVYSISILSDGIILFLYTLFFFLLIWSLE
                     KPTFKRWIITGIILGFCALSKPTIGIYLPFLFFGLFYLKDKKLLPLNLSHQKQALITF
                     FLIIFVSGITILPVTIRNFIVEHKFVPICFNGPINWAIGNSSDSIGLFYYPKGEVLSV
                     FSLPFWKLFLTKLYLFFTSYEWPQNMNVYLVEKVIPFLKLAFIKFGFVVPVGVFAFFI
                     LFKNFKKYYFLITFTFFNVLWVVLFFITDRYRLPAVSCFIVLCSYFFIWVYEKIFREK
                     KLFLPFLTCFFILIFAYFFDITPGPLIPDESKKIFALLSVKNIQNDLKNGNIKKAKKE
                     SKKYNEILPDDPKSSYLLACVYYELKDIDKCFYYLEKTLEISPDYEPARKFLNDILK"
     gene            28010..28291
                     /locus_tag="PLW95_02375"
     CDS             28010..28291
                     /locus_tag="PLW95_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21511.1"
                     /translation="MENVICPVCKKNEVEMEEKYGEFTLYKCRICTLEFFYPLSYKSS
                     LYEEFYSFGYGNTVIKNKQKFPILFPYVLFNYIRFFNKFSDTSLLVIAR"
     gene            28288..29460
                     /locus_tag="PLW95_02380"
     CDS             28288..29460
                     /locus_tag="PLW95_02380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOV21512.1"
                     /translation="MKKEKICIICNDPFSDYLLNRGKECIEKYFNPLLKFETHIISFE
                     DCYKYQTRKIGTLIIHPFCEKQFKKTLFYPWRVIRTLSRHLLRGIKIIKIIKIEKIQL
                     VRGYGISNLAFIGLIASKITKKPYIISLHNDYDYMFKLKGKTKRIMWHFLEKLTIKFS
                     DFVIVVSPFLIEYAKKHGNKKVVYIPNPLDLEKFIEVGKKRTFNEKEEKTLIFAGRFF
                     DPQKNFYRLLVAYSKIKSELREKSKLFVLGDYGGKLGYYKELTKKLGIEKNVEFIGRV
                     PRTEMPLWYMKGDFLLLPSLYEALPNALIEGMATGLPVLTSKHPSCTYLVDESNGVIV
                     NPLNVDEIKEGIEKLLSMKQEEYKEKAENSVKKVKNFNDEEVFKKVAFLYEKLIKR"
     gene            29463..31340
                     /gene="asnB"
                     /locus_tag="PLW95_02385"
     CDS             29463..31340
                     /gene="asnB"
                     /locus_tag="PLW95_02385"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOV21513.1"
                     /translation="MGSICGIYNFDEKKIVDKKILKKMSDKLKHRGTDKEGYYLNRNI
                     GFGHKQFMIGYESREQPISNENETIWIVLDGEIYNYVELREFLIKKGHKFSTKSDEEV
                     IIHLYEDYEGECLQFLRGMFVIAIWDERKKRLFIATDKIGQKTLYYINKDGKFVFASE
                     IKALLEFPEYEKEINFEGINYLFAYEWWIPWPYTSFKNIYKMLPATYFMIEGNKLTEK
                     KYWTPDFINKTKKKEKEIIDELYNLIDESVKIRLRNNIPVGALLSGGVDSSGIVAFMS
                     NYLSHIKTFTIGFKNKEKLNQEFLRAREISEMFRTEHYEVLLTDEDLIENIPQLVINH
                     DEPYVCITGLYTYFLTKFIKEKGISLTLSGNGPDEIFWGYSSGVDLKIKSIVNGIAKY
                     VPDEILNIFPKFLLPKNIQIRIENLKKPFSIKLSEGINKKFEENTFGLFKKNFPKINK
                     NYGLLAKEYFSEAEGKTDLITAYSYTALMLTYAFGLTIQPNLDGMANTVEIKSPFLDQ
                     KIIEFAFSLPLEYKVKGFTPKGCKYIFKKTMEKKLPKNLLYAKKLAFGTDIKWEKLIV
                     EKFGEKIKAEVLNGWWVKNNFFSREYIEMLFSKMPETKTSLQILRLYTLELWYRIYFK
                     K"
     gene            31337..32293
                     /locus_tag="PLW95_02390"
     CDS             31337..32293
                     /locus_tag="PLW95_02390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOV21514.1"
                     /translation="MRKEYLLKFKDQIEIWDEKWNTAEIEKEIYEAKNSPIADIIKIF
                     LPSQGKILDVGCGLGGYLIWLKSEGFNIYGIDFSKEAIRRLKIYNPSLDVEISDCEAL
                     PFSDNYFDVYISLGVLEHIESGCLKALKEANRVLRDNGIVIISVPFLNLYRLIKDFFI
                     FKLMGKESYVLTNVEEKNIVFYRRKLYKEEKKYSFFQYYFSLSEIRKLLKKSQFNIIL
                     WTPYSVLPGILEIKLFQKLYKKFILSKSKENLYKIKRIENSKKINNKSIKKRKFYFIL
                     AKRFYKLLSWEKSKNLLEKNILNIFRHIFGHMILIIGVKNDN"
     gene            32283..32420
                     /locus_tag="PLW95_02395"
     CDS             32283..32420
                     /locus_tag="PLW95_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21515.1"
                     /translation="MITKRENKYIKSRLSLPWIEYIKIYTSLKGKKILDVGCWNKWLF
                     I"
     gene            32431..32583
                     /locus_tag="PLW95_02400"
     CDS             32431..32583
                     /locus_tag="PLW95_02400"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOV21516.1"
                     /translation="MEEFIEKVEFFIKNNNLREKIGKANKEKIIKNYNWIESAKKLKT
                     VYEKIL"
     gene            32580..34037
                     /locus_tag="PLW95_02405"
     CDS             32580..34037
                     /locus_tag="PLW95_02405"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOV21517.1"
                     /translation="MRESIKVAKSSAKLVSAEFINIIFGIFYFLIITRTFSKIEMATI
                     AIMGVITNISAVIIGFGMHATSVKLIPEHLSKEENENVSEILQIAYILVIFASIFFVF
                     FTFLFSSQISSIFFKSPSYSNLIKIISFSIFTNKLYETSFHNLTAFQKFTEISLLQIM
                     NNVFVRIYALFLYFSSGIEGYLIGLITGQFIISICMIFLTKQYLFKKIKEHNFKKILK
                     FSFPFYLNGYIGYGANHADSLIVSIFLSPSLIATYYVVRRFIDYLEIFITSILTPLSP
                     KMVEMKKNGKETLEKIFYKSTRYLSFILIPTCLFMISISYFLLDIYGKGKYLSGLPIL
                     IILSLGMIFNGFYLLLRGTIFVCSKPIETTKVELFKACINILLPLTLIRKFNILGISC
                     GKFISLFTSVFFAYYIGKKLLILKFDWENIKKSFLISFFSVILIVILQILFYTPFWMI
                     LYICVGITVFSLLFFKFTTQDEKLFFKEILLKWNN"
     gene            34025..34951
                     /locus_tag="PLW95_02410"
     CDS             34025..34951
                     /locus_tag="PLW95_02410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOV21518.1"
                     /translation="MEQLVSIILPVYNGERFIERAIRSVLAQTYKYWELIVIDDGSTD
                     KTSEIVKRYLNEKVKYLYQKNSGVSIARNKGIEISNGDYIAFLDCDDVWKPEKLQVQL
                     SKFMENYNVGLIYTDVFIVTERGKILGKFSQFNYPYSNKVTKKLFLQNFICTSSVIMN
                     KEILKKTGLFDPDIKIGEDYDLFFRLSLYTNFYYVDSPLLYYQISEKGLSRNKIKMWE
                     ENIKILDKWLKKYPELTKILGTKTIKEKKSNLFILLGKEYFENGKFNQAQKTFYKAIT
                     YKKNIKGISLFLLSFLGKFGKFLLSLKQRVLK"
     gene            34948..36084
                     /locus_tag="PLW95_02415"
     CDS             34948..36084
                     /locus_tag="PLW95_02415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOV21519.1"
                     /translation="MIYFIIRDFWGKGGTEKVAQTLAEKFYKKEKKIKIITSGKRIFR
                     KKIWNEIIVIELFSPNIKYIGTLFYYFFLTTYLFLTMRQYKVILVFFLKHSAFISIFI
                     SKILGKKVICRIAGTGKYGDIQTLKKIKFHQILLYILRKADKYITLTECMKKELLEEK
                     FDPKKIEIIPNGVDIKKFRPPDNKEILKEKIGIKKKKILVFIGRLSEEKGLKILLKAI
                     KNDIFKNTLLYIVGKGYLETELNEFVKTNCITDRVVFTGFKQDVVPFLQVADVFVLPS
                     ISEGFSNALLESMATGLPVVATKVSGNIDIIENGINGFLVEPENSEELAKALEKILKD
                     ENLARKMGEENRRKIVENYSIDKIVEKYIALYNNLISKSIIKQN"
     gene            36185..36772
                     /locus_tag="PLW95_02420"
     CDS             36185..36772
                     /locus_tag="PLW95_02420"
                     /inference="COORDINATES: protein motif:HMM:NF024734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HOV21520.1"
                     /translation="MKYIKIRELKEKIYFTTNEIAEYLGIKKESARVLCSRYSKKGLI
                     IRLKRDLYILREKWETLSIEEYFNIANIFQVPSYISLMTGLYYHQITTQVQRRFYESI
                     SLKRSICFSIEQTEFHYYKINRKFYFGFFKKDKIFIAEKEKAFVDILYLTSLGKYKFD
                     VSSVDLRKLKKNKIEKILKIYPEKTNKIFIKIWKS"
     gene            36760..37425
                     /locus_tag="PLW95_02425"
     CDS             36760..37425
                     /locus_tag="PLW95_02425"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOV21521.1"
                     /translation="MEELIQQEKFEIEVLEYLNSKKYLDKMIFVGETMLRLCYGLNRF
                     SVDLDFWIIKKIKFDKFFLDLKNFLEEKYIIKDCANKFSTILFEISSSEYHRNLKIEI
                     RKEIKKIKYEKAIAFSKFSNIQVLLNSIDLNDMMKFKIESFLSRKEIRDCFDIEFLFK
                     RGIPINLNVKQAKLLLKEIEKLKENDYKIKLGSIIEREVRNYYLKKNFYILKMELMKI
                     LIT"
     gene            37435..38541
                     /locus_tag="PLW95_02430"
     CDS             37435..38541
                     /locus_tag="PLW95_02430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOV21522.1"
                     /translation="MKKICYVIGTLKVGGAEKQLYYLVKNLDKTMFSPVVIALRGGIL
                     EYDFKEICSLKIVGKRWKIDPLFFLKLVPILKKEKVDILHTFMFTANTWGRLAGIICK
                     IPVKIVSERSIDLWKKSYHFLIDKILGKFTDKIICVSEEVRNHYLKKTKLPSDKFIVI
                     ENGVNLQEIEKVKEKKEIKEEFGIQDDDFVILTGGRLCKEKSIDFFLSIVPELKKQMT
                     KLKILIVGEGEEKENLFNITKYLKLEDNVIFTDYRRDILSIIKISDLVVLTSKWEGMP
                     NLILEGMALKKAVISTNVGGCKEIIQNNVNGFLIEYGDKKHLIEKILFLYKNSGEREK
                     MGESGYKIVKEKFDLIVKIKEYEKIYSSFKIGKN"
     gene            38544..40058
                     /locus_tag="PLW95_02435"
     CDS             38544..40058
                     /locus_tag="PLW95_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21523.1"
                     /translation="MKNKIIIFSAILLTFLIWIPYIFPYFSNVDEPAYFLNALSVFKG
                     NVLYRDVFENKGPIMTLICLFFIKISPENYTFLFHLTLFLLLFIESILIYKVLVKLKL
                     KNLAIYGFFYPVFLSLFYPGNMTIGGEPFISFLLLIIFYIFLLFPNKIIPIFVIGLLS
                     GIIVLFKPFGIFLILLLIFLIYYKSKSIKKISLFVFASIVPLLFFLLYIFKYHIFHDF
                     LLWAIKYPVFLSRTVPTLKECYFIFPMLGRIFLLIPFFTVFGIYEIIKKNKNREEIYI
                     ILFLIFTALWGALHGLPFPHHYRLLFPFLFIISLIGYFNIVFSQKFQLKNILNIFIIF
                     SILQGFFYWNGIDFYKKWIEFLSEKKWTKEYEIKSNSEILEFLNKNTSPEDKIVIWGH
                     NPKLYLLSKRDPGTKFKAWFDPVIGIVFYDIHKIQQFPNAEELFIFDIKRNNPKYFID
                     ATKCSSIGIPYYSVDKYYKIYEFLLNNYKRIKELNGYVIYQNKKNVCNMWNNKK"
     gene            40027..>41062
                     /gene="asnB"
                     /locus_tag="PLW95_02440"
     CDS             40027..>41062
                     /gene="asnB"
                     /locus_tag="PLW95_02440"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOV21524.1"
                     /translation="MCAICGIIKNEGKVEEKLLFEMTCLMNYRGPDEEGYFIEENVGL
                     GHKRLSIIDLKTGKQPLFNEDKKIVLICNGEIYNFKELRENLEKKGHKFRTNSDNEVI
                     VHLYEDRQEKCIDYLRGMFAFAIWDSNEKQLFLVRDRLGQKPLIYSLVGKDIVFSSEI
                     KSILLYPEIKKEINLEALSDYLTYQAIPSPNTIFKDIKKIPPACYLIWKNGNIKIEKY
                     WDIDFTKKNYFKNENDYMELLWENLKEATKLRLISDVPLGAFLSGGIDSSTIVGIMSE
                     ICEIPVKTFSVGFDVKEYSELEYAKIVAKKFGTQHHEFIVKPDIINILPKLVWHYNEP
                     FADSSMIPTYY"
BASE COUNT        15764 a         4880 c         6393 g        14025 t
ORIGIN      
        1 tatttttgag gaaactactc tctttcaaag agattatttt tgaggaatgc cgtgggaaat
       61 taaatgataa aacactaaat aattcaaaat ttatattaga aaaatctatt ccaccaaaat
      121 atataggaat ttacatcttt gaaggaatat aaacacaatt acttttaagg atattagaaa
      181 ttagaagtat cacttataat ttgctgtgtt ttgaatacca aagttaacta aatcaatcat
      241 ttaagtaata tattttcaat aaaacaattt ttaaagaaag cccaaaattt gatttttgta
      301 tgatgcgaac tataatacaa aaatatatta aggaggcaaa ttttatggaa aaattacaat
      361 tatgggaaga cgaagtaaaa aaattgcaga ataatattga agcacaaatg tctcttaccc
      421 attggaaatt taagcaggga atagtaaaaa atggagaatc tttagaatta gatgatactg
      481 attgggaaga taaaacaact actacatggt cattaaaaga aggtccggcg tattttagaa
      541 catacctgac agttcctaag aaaattgagg gaatagatat agaaaacagt gatattgata
      601 ttactttttg tttccctagt ggagtggaaa tgtttataga tgggaagaaa gtatatactc
      661 ataagttctg ggctgataaa ataggcactc ccatacctct tatacaaaag gcaaaagcag
      721 gggataagat gctaattgta tttaaaacac ctgcgggaga tggattagga tacttctggg
      781 caaaaattag tataagtaaa atagaaacta ctctttttga gattaactca attctttatc
      841 aacttaagtt tgctcttgca cttgctggtg aaaaaaaatc attgaagaag tatgtagaaa
      901 aagcaataaa tataatcaat cttgatgata ttacacaaag gcattgggag aatgttttat
      961 gtgatataaa agaagcagaa aaaatacttg agatattcag aaatgatgca aaaaaattct
     1021 gtatccacct tataggtcat gcacatattg atataaactg gctctggaca tatgaagata
     1081 caataaatac ctgtctgcga gattttgaaa cagtaaccaa acttatggag aaatatcctg
     1141 atttaacatt ttcacagagt caagcacata tatataaaat agtagaagaa cataacaatg
     1201 aactttttaa taaagtgaaa agtagaataa aagaaggtag gtgggaactt acagcatctt
     1261 catgggttga aagtgacctc aatatgattg atggagaaag tattattagg catattcttt
     1321 attcaactaa atattcattg gaaaagttga atacaaaaac aaaaattttc tggtctcctg
     1381 atacatttgg acatccgata acaattcctt caatactagc atcagcaggt atgaaatact
     1441 actattttat gagatgtggg aagggatttc cccttttcag atggaagggt atagatggaa
     1501 gtgaagtaat ttcgttcaat agcatttaca acaattacat cttaccagaa aagataatgc
     1561 caaattttat agaatatctg aataaatata aactccataa ttttcttttt gtttatggta
     1621 taggagacca tggtggaggt cccacagaaa aagatataaa aagaaaaaat atgatggaag
     1681 aaaaacctgt aattccaaca cttgtatttt ctacagcaga aaaatatttc aatgaaattg
     1741 aaaaatacag aaataaaata cctatttgta aaggtgaatt aaacaccgtc tatgaaggat
     1801 gctataccac acacagtgat ataaaaaaga acaacagaga atgtgaaaca cttatgcaca
     1861 ctattgaaac actttcagca ataacaggga tagataatga aaaagaaaag aaaatagagg
     1921 agatgtggca atcaattctt tttaatcaat tccacgatat ccttgatggt tcagccatac
     1981 actcttcata tgaatattca tcaaaattgg cagaaggagt aaaaaagaac ggagaaatac
     2041 ttattaaaga gatgatggat aatttatctt ataaaaaaag taaagataaa attattgtat
     2101 ttaatccatt aggatggaaa agacgattca ttattccaaa ggaaataata attgatgaaa
     2161 tagatggtta tggttataaa acaattttat ccaaaaacaa aatgcaatgt caatcaaaag
     2221 tgataaaaaa aataaataat gattatgaaa ctgaatttta tagaattaaa atagaccata
     2281 ccacaggtct tatcagaacc ctttatgata aaaagaataa atgtaatgtt cttgtacctt
     2341 tgtatcccaa gtcagatgat atttcatgct ggttggcaga aaaggcaggc aatcttataa
     2401 gtgtaaattg ggaacaaccg cactctatga gtgcttggaa tataggaaac atataccgcc
     2461 aggattattt acttgatgct gaaagcattg aaattgagga atatccgcta tatacagtta
     2521 taaaaataaa aagaaaatat atggaatcag aaatattaca aaaaattata ctttatagcc
     2581 aattaccatt tattgacttt gagaatgaaa tagaatggaa acaagaaggg aataataaag
     2641 atggtgtacc tatgcttaga gtaagtttta atgtaaatat ggaacatcca gaagcatatt
     2701 ttgaagtgcc ttttggagca ataaaaagac agataacata ccaggaatat cctgcattaa
     2761 gatgggcagc ccttaaagat aagaattact gggttacact ttttaataaa gataaacatg
     2821 gatattacat aaatggtaat aacttatctc ttacactttt gagaaatcca tatgaaccgg
     2881 atacaaaacc tgacagtgga tttcatcata tttgttatcg tctacaattt ggtaaattaa
     2941 atgcacttga aataacaaag ttagcaatgg aatacaatac tcctattata acaatatctg
     3001 gagaaacaaa gtcagaagaa ttctatccat tcttaataag agggaatatt cttcccactt
     3061 cctttaaaaa agcgattggt cgagagagtt atatattgag aatggtggaa atgtttggga
     3121 aagaacagga tattgaaatt aactttacaa gaccggtgcg aaagatatat atttccgatg
     3181 caattgaaaa tagaataaaa cagataaaat gtagtaaata taatataaaa ataacggtcc
     3241 acccttttca aattttgacg ctggatattg agttataaaa ataacttata ttataaatac
     3301 ttagttccac aggttccctg tcggcgtagc ccgtgagatt aaagcaagag ataatgttaa
     3361 tttttattat gctcttgata taaaaatgct atcaattggc tattttaaat tttaatgaat
     3421 ttatttggtg cggattagat actgttctac aatacccatt atattcattg ttacccagta
     3481 tagaaggaga cctgaagaaa aattataaaa gagaaaaaga aacataagag gcatcataat
     3541 agtcagtgct ttttgggatg ggtctgatgg agtaagtttt tgttgccaga aagcagttaa
     3601 agtcattaaa acaggtaaga tgtttaatgg gaaatgtcct accctcaaaa ttgtatcagg
     3661 aactgaaagg tcttttatcc ataacataaa tggtgcacct cttaaaaaaa tagaattcct
     3721 taaagcaata aaaaaaccaa taaaaatagg aaattgtata agcattggaa tacatccacc
     3781 aaacggattt attttgtatt ctctataaag attaaacatt tctttttgca ttttttgagg
     3841 gtcattttta tattttttct gtaaatcctg taaatatggt cttaactttt gtatttgttt
     3901 cattgaccgc aaactatttc ttgttaatgg gaaaaataca attttaatta ttaaggtaag
     3961 taaaataatt gcccagcccc aattggggat aattttgtga atattatata aagcggcaaa
     4021 taaaaacctg cccattagaa caaaaaaacc atttccaaaa atattttcct ttacctcttt
     4081 tgatgcaatg aaataatcat atggtcctgc ataaaaattg aatttaagta ttttttcttc
     4141 atttttttct atgggtaatt tatttacaat aaatccagca gtaaaaatat tttcttcatt
     4201 tgaaataaaa gcatccattg gtttttcagt tctataaagc agaagggcat attcactttg
     4261 aagagcaata aaattacaat tttcaaatgt ttctttgcct ttaacttttg ttatatttct
     4321 ttttattatg ttgttattac ttccaatgag taattcagtc cttaaatatc tattataatt
     4381 ctgggatttt tttatttcat ttttattaag agataaaaga tttattatta agtcatcaac
     4441 ttcctgtttt tcattactat tatttttgat ttttatttca acttcaaatg tatgtgaatt
     4501 tgttggtata gttatgtctt tctcaatata taatttatct atatttgtcc aaagggtaat
     4561 attattttct ttttcttcaa tattccataa tttttcaatt gcttctaatt taccttgctg
     4621 aaatgtgaga aataaataat tattttcaat tatagggaaa tatttttcat catctctatg
     4681 gtatttttta agtcctataa ggtcaataaa accaccattt tcatttattt ttattataaa
     4741 attattattc tcataggcac ttaatttctt ctcgattttc cctatttcac ttggcaactt
     4801 tgtttctgta attttctcat tttctgatgt tttttcttcg tttgtagtgt tttcaataat
     4861 ttgttgttgt gttttaacag gttgtttttg agaagtaaat ttaccaaaga aaattattat
     4921 taaaattgat aatacaaaag caataattag tcgtagttcg tctttcattt tatccatctc
     4981 tcaattgggt catatccacc ttcattaaat gcattacatc ttaaaattct ttttatacca
     5041 attaaaatac ctttccatcc acatttttca attgcctgta ttgtgaattc agaacaatta
     5101 gggtaaaatc tgcaatttct tcctaaaaat ggagatatta tagtttgata aatccttata
     5161 aaaaaaatta aaatttttcg cattttgcta tttctaccat tttttgaagt tcttttttta
     5221 tttcttcttt acttttatta tttacaactt catgtctcaa aacaaacaaa aaatctccac
     5281 ccttcaaatt ttttctccaa tattccctta taactcgttt tacataattt cttttagttg
     5341 atttccctat ctttcctgaa aaaataaatc caattttata gtcatcactc cctttaaaat
     5401 ataaatcaaa aaacacccct tgtactttct ttccttcttt aataattttg tatattcttt
     5461 ttcgcattaa ggagtgagta tttttcgccc tttttttctt cttctgctta ttacttttct
     5521 tccatttttt gtttgcattt ttgcgcgaaa tcccatcttt cttttttttc ttaatttatg
     5581 tggtttatat gttattgaca tttctcctcc tgaaaaatgt gtaatttacc actgtcattt
     5641 aatagtaagt agagagaatt ttcctattta aaatccatcc ctcgggcgta gccctacggg
     5701 cgagcctacc cttcctttat taaaggaagg aactattcta tctttcaata acggatgaag
     5761 aaattttgct tttcttgtct ttatactttc aggcattgta gttgactgag tattataaat
     5821 tttactacaa aataatgaaa ctttcaataa aagcataaat ttattatgta aagtttttat
     5881 ataacttttt aagttctttt aaaaatttgt catcagtgag aataagattt aaaggtgtaa
     5941 ttgaaataaa tccacttttt attgcatcaa catcacttcc tttttcccct tttattttga
     6001 aatttccttt tagccaaaaa tagggtacac ccttagggtc tattcttttt tcatattctt
     6061 caataaatct tgatttactc tgaaaagtaa attttattcc ctttattgtt tcaaggggaa
     6121 gatttggaat atttatatta aaaattgtat cttctggtaa tttaattttt attaaacttt
     6181 ttataatttt tttaagtacc ttttttattg aaagaaaatc aaaattttta taatctgtta
     6241 ttgaaattgc aatggacggt ataccaagaa ttgcgccttc tgctgctgcc gcaacagttc
     6301 ctgaatacaa aatatccatt cctaaatttg gtcctaaatt tactcctgaa ataataacat
     6361 caggtcggtt ttttaatatt tcttttactc ctattttcac acaatcaacc ggggtaccat
     6421 ttacaatata acaattagag taaaaaaggt ctattttttt aattctaatg ggattaaata
     6481 aatttatggc atgagaaata gcacttgttt caaattctgg aacaactata attttttcaa
     6541 ttgaagaagg taaacattcc acaagtattt ttaatccctt tgaatatatt ccatcgtcat
     6601 ttgttatcag tactttcatc ttttttcatt cccctatcag gttgcctttt taatggagca
     6661 gaccggactt gaaccggcaa cctccagagt gcgattctgg cgctctccct ttgagccact
     6721 gcccctattt ctcttattat tatattttta acttataata aattcaagta attatatgct
     6781 taatgattac tatttatagg cataacttct actgatattt ataaaaaata tgatataata
     6841 aagaacagga ggacagaaaa atgaaaaaac ttattgcagg gattattggg gcaggaggta
     6901 tatgtgatta ccatattttg ggttatttaa gtacagatgt tgaagttata gcaattgctg
     6961 accctgatat aaaaagagca gaagaaagag gtaaaaagtt tggaattaaa tatgtattct
     7021 cttcatatca ggaaatgatt gaaaaactgc ctgagatttc tcttataagt gtttgtgttc
     7081 caaataaatt tcattctgaa atttccatat attgtctgga aaaaggtaaa aatgtatttt
     7141 gtgaaaaacc accagcactt aatagcaaag agacagaaaa aatgaaaatt gcagcagaaa
     7201 aaaacatgaa aattttaatg tttgatttta acaatagagc aagaccagaa tcacaggcac
     7261 ttatgaaata cataaaaaat ggtgatatag ggaaaataaa ttcagcacag gcattatgga
     7321 taaggagatg tgggatacct ggttttggag gatggttcac tcaaaagaaa ttatcaggtg
     7381 gtggtccagt aattgaccta cttcatatga tagaccttgc attatatttc atggaatttc
     7441 cagagccgga ctgggtatta tctcaaacat tttatgattt ttcaggtaat cctgatttta
     7501 agggaccatg gggaattcca gatgttgaag gtggaataat ggatgttgaa acatctgctc
     7561 attcatttat aaaatttaag acaggacagg ttttatttgc aagaaatagt tgggctgaaa
     7621 tgaataaaag ggaagaagtt tctgtgatat ttcaaggaac aaaagcaggt gggatgatta
     7681 gacgactttt tggtagagat ggaattgatg aaacagcaat agatgattgc gaactttata
     7741 ctatggaaaa tggtaaacca gtgaataaaa aaattatagt tcaaccagat gaaaaaatgg
     7801 gaagagagag agcagttata aattttgtta atgtggtcag aggaatagaa gaaccatatt
     7861 caacactaga acaggcagta attttaatga aaataattga tgcaatttac aaatcaagtg
     7921 aaacaggaca accagtaaaa attgcaacta ctcagtcaac ttccgttgac tgaaaacagg
     7981 aaacataaat gtaaaaggaa aatctatcct tatattcttc aacttttgca aaggtgggta
     8041 taggtaggga agattttaaa aaatttatct ctaccttccc ctccacaaag atggatgggc
     8101 aggctttaca aagggaagag aaaaaattta ataaaattac tttttgaaga taatatatta
     8161 ttatgaataa tgaaaaaaaa gtaatttgtc catattgtgg aaagtaaaat aaagagggat
     8221 tgaattataa actctaaact ttttaaaata aaatatgtct aaatataaat tttaaaggca
     8281 aaaaatgaaa aaaggatatt tttttataat agtgggattt ttatttattt tatgtggttg
     8341 ttctgttaaa tattatgaga aaagtgctga aaaagatata aaagaaattt gggacaaaag
     8401 ttataaaaag gttgaagaaa aaaagttgcc tgattccata ttacaagaag atgtatttcc
     8461 tgaaacaaga gtgcttgacc tttcatctgc aataaaaatt ggtcttgaaa ataatagaaa
     8521 ttataaaagc aggaaagaaa atgtttatat aaaaattcta gactatacat accagaaata
     8581 tttattaaaa gaaaggtatg gaattggtgg agatataagt tcagaaaaaa gtggtgatga
     8641 aactatcggg gctgatttga attttaattt aataaaatta ctttcaaatg gtgccaaaat
     8701 tagttttgat ataacacagg actttttaaa atattttacg ggaaacaaag aaaaggattt
     8761 tcagacattt ttatccttaa attttttcca gccaatttta agaggagcag gaaaagatat
     8821 tgctcttgaa ggtctcacac aggcagaaag aaatgtaata tatgaaataa gggatttttt
     8881 aaggtatgaa aaacaattat ccacagatat tgcaaaaaaa tatctttctc ttgttgcaca
     8941 gaaaaaaaat atagaaacct atagaagaaa ttacgagttt ttaaaaaaga taagagaaag
     9001 aaatgaaaat ttagcgcaag caggacgcct ttctccaatg caggtagata tggcaaatca
     9061 ggatgaatat tctgcttctc aaagatggat aactgcttca aataattata aaaatgcagt
     9121 tgactcattt aaaatatatt taggtataca gccaacagaa aaaatagaaa tagaagataa
     9181 acaagtagaa gatgttttga gtaaaggcat acaaaaattt gatattgacc ttacaagtgg
     9241 aacagaagtt gctttaaaga accgacttga cctgattact ttctatgata tggtagcaga
     9301 tgctgaaaga caggttaaaa ttgcaattaa taatttaaag ggaactctta atttgaatat
     9361 gagtatagaa agtggaacag aaaaaaaatc aagaccaaca tttgatttaa caaaaccaga
     9421 atattctgct ggacttgaat ttgaattacc tattgataaa ttaccagaaa gaaatgccta
     9481 taaaaaatcc ctgatttcac ttgatagagc aaagagaaat tttgaagaaa aaaaagataa
     9541 tgtaaaactt gaagtaattg aaaattatag aaatttagaa gaagcatatc aatcacattt
     9601 aattcagaga aatagtttaa atttggcaga aaaaagggtt gatagcacag accttctttt
     9661 tcaggcagga agagcagaaa caagggactt acttgaagca caaaatgctt atttatctgc
     9721 agaaacatca ttaacaagtg cttcaataaa ttatataagt gcatatcttg attacctttt
     9781 aagttgtgaa atgatagaag tggatattga aaaagtctgg aaaggagata aatatgaaga
     9841 aatttttggt aaaattgata aaaaataaat ttttcattct tgggattttg gttattataa
     9901 ttattttttc agttgtaaga tatttaaaaa taaaacaggc aaaatcacaa cttcttacat
     9961 atacggttaa aaaacaggac ttaattattt cagttataga aaatggaaat gttactgcct
    10021 taaaatcact taaaattatc aatcgtgttc caggaaccag aaatattctt ggagttgttg
    10081 atgaaggaac ccagataaca gaagaagatg ttaaaaataa aaaaatttta ttgaaattag
    10141 attcaaacga ccttgaagat agattagaca cattagaaat tgatgtagaa aatagtttgg
    10201 ctgcatatac acaggcatta caggaacttg agattacaaa aaagcaaaat gaaagtgata
    10261 taacgaaagc agaattaaat gttaaattcg cgaaaatgga tttagaaaaa tatttaggaa
    10321 acaaaatttc agaggcagta ttaaaagaaa aagaagttga ttataaaaaa attataaata
    10381 gtcctgattt aggtggagag gcatggaata ctaaaaaaga ccttgaaaat acaatagata
    10441 ttgcaaaaga agaacttgaa agagcaaaaa ataaccttga atggagtgta aaattagcag
    10501 aaaaaggtta tataacaaaa aaagaccttg aagcagacca actttcctgt aaacaaagac
    10561 aggcaaattt agaaatggct gaattaaaat atgcattgtt tataaattat gactttccaa
    10621 aaaaagttga acagttatta tctgactaca aagaaaacat aaatgaatta gaaagagtga
    10681 aagataaaag taaagcaaaa ttaatacaat cagaatccaa tatgagaagt aaaaaagcaa
    10741 catatttgtt gagaaaaaat acttttgaag aagtccagga acaaattaac caatgtacaa
    10801 tttatgcaac tcaacctgga tttgttgtat atgcaacaag ttcaaggcca tggaggactg
    10861 aaggtcctat acaaccagga acgagtgtcc ggcagtttca agagctatta aatatacctg
    10921 attttaatta tttaggtgtt gatgttcaag ttcacgaaac aaacatcaaa agaataaagg
    10981 aaggtcaaaa ggcaattata actattgatg catttcctga tacaaaattt gaaggaactg
    11041 ttaaaaaaat ttccttaatg cctgatgcag tgatgaagtc attaaatcca gatatcaatg
    11101 tttataatac tcaggttgtt tttgataaat ctgcttcttt tttaaaacca ggtatgtctg
    11161 ctcaagtaga aataatagtt gataaaaagg caaaagttat tgctatacca gttgtttctg
    11221 ttttatttga gaaaaatttg ccattttgct atgttcttaa aggaaataaa attgaggaaa
    11281 gaaaacttga attaggggat agtaatgaca gaatggtaga agtaaaaagt gggctaaaag
    11341 aaggagaagt tattgttatg atgcctgaaa cattaggacc tgaggtaaaa aaagaagaaa
    11401 tgtatgaaaa agggaaaatt actactgaaa gtaaagaaaa acaggtaata gaaaatgtgc
    11461 aggaaataga aagcaaacaa agcacagaaa ttgaacaggg aatacagggt ggtcaaaata
    11521 tagaaggtaa acagggagtg aaaagagggc agaggagaga acgaggggaa agaaaagtca
    11581 atgagtgaaa ataataatgt tatagaactt ttgaatatta aaaaaattta taaaatggga
    11641 actattgaac ttgaggtatt aaggggtata tctttatctg ttaaaaaggg agaacatatt
    11701 tcaattatgg gtccatctgg aagtgggaaa acaacattat taaatatatt ggggtgttta
    11761 gataaaccta cttatggaca atattttttt caggacaaag atatttcaaa attagatgat
    11821 aactcgcttt cagaaataag gtcaaaatac attggtttta tttttcaaca atttaattta
    11881 attcctcaat taaatgttat tgaaaatata tctcttccac ttttttacca gggaattgat
    11941 gagaatttaa tgatggaaaa agcaaaagac tttgcagata tggttggact ttcagatagg
    12001 ttatatcaca ggccatctga actttctggt ggacaacagc aaagagttgc tattgccaga
    12061 gcgcttgcaa atgacccact aataattctt gcagatgagc caacaggtaa tcttgacaca
    12121 actacaggaa aagaaataat gaaacttctc tgtcggttga acgaagaggg gaagaccctt
    12181 ttagtaatta ctcatgatat aaacattgca aattatagta aaagaattat aaaactgctt
    12241 gatgggagaa ttgtaaatga aagttaaaat atatcatata ttaaaaattt taaaattagg
    12301 tctcaaaaat atttttcttc ataaattaag gtcatttctt acaattttag ggattgtttt
    12361 tggagttgca tctgttattg caatgctttc aattggagaa ggtgcaagtt atgaagcaag
    12421 agaaaaaata aaacaattag gaagcaataa tataatagtc agaagtttta atccaccttc
    12481 atctataaaa acatcccaag atgctgctgc tattggtctt tatggattaa aatttgatga
    12541 tttagaagaa ataaaaacaa ttccttctat aaaaaatgtt gtgccaacat gggaatgtaa
    12601 agaagatata tccagtgaaa atggacagat aactggtaga attgttgcca cagagccatc
    12661 ctttttaaaa gttatgaatc ttgaacttat ttatggcaga ttttttacag atattgaatt
    12721 gaaaaattca aggaattttg caattattgg atattcaatt aaaaagaatc ttttccctct
    12781 tgaaaatcca gttggaaaaa caataaaagt ccgaagttat tatttcaaag taattggtgt
    12841 tgttaaggaa aaagcagtta atttaacagg aacaagtttt gaaagtgaag atataaattt
    12901 tgatgtatat atcccattta caacaggtag agtttatttt ggaaaatatg aaattgcaaa
    12961 taaaagaggt cctgtattta actgggaaaa aaatttagtt gaatatcaca gatttataat
    13021 aaaccttctt gatacatcaa aaattttaac agttgcctct tatgttgaaa aaattttatt
    13081 aagtaatcac aaaagcagtg attacgaaat tttaatacca ttacaattac ttcaacaagc
    13141 agaacatact aaaaaaatat ttaacattgt tttaggttca attgctgcaa tttcccttat
    13201 tgttggtggt attggaataa tgaatattat gttagcaaca gttactgaaa gaacgagaga
    13261 aataggtata agaagagcaa ttggtgcaaa gaaaagggat attataaacc agtttatttc
    13321 agagtcagtt attctttcag gaactggtgg tattattggt gttttacttg gagttataat
    13381 tcccaaactt gtaacaaaat tttcaggaat gacaacaatt attactttct ggtcaataat
    13441 actttctttc acaatttctg ttgctgttgg aataactttt ggaatttatc cagcaagaaa
    13501 agcagcagaa cttgacccaa tccaagcatt aagatacgaa taataataag gtggaggacc
    13561 gtcctccaca ggaggacggt cctcagtgta gaaatgaaaa tatacataag aattttaata
    13621 gacattatcc tggtagtttt tttatttctt gtaggttatt tttttatata taaagaaatg
    13681 cattttgtga aaataatttc ttcttcaatg gaaccaacta tttgtgttgg tgatagagtt
    13741 ataactgtaa aaaagggtgt agtgcaaaga ggggatattg ttgtaataga agcacccaaa
    13801 ggtaaaaaag aattacttac aaaaagagta ataggacttc cttctgaaat actggaaata
    13861 aaagatggta gtgtatttat aaatagaaaa aaacttgaag aaccatatat aaaagaacat
    13921 ccgagatatg aattaaaagt gaaaatacct gaaaagaagt attttttact cggtgataat
    13981 agaaacaaaa gtgaagatag cagtatatgg gggccggttg acgaagaaaa tattaaaggt
    14041 aaagtaattt gtcggtactg gccactaaaa aatttttcaa tattttaata tttttctggt
    14101 ggtgtccaat cccagagagg aagttctttt ttatcaacag gacatggacc tcttggggct
    14161 atccactcgt gattggggaa tcttggacag tcaaatttca ttcttgttat tgccttatca
    14221 attggacatt gatgataaag gaaatgaaag atttcaaatg tttgacagcc atctgatatt
    14281 cttccacaac ttgtacaggt aaattttgaa attaatgctg ctccaacata aggtatatag
    14341 ccatcaaatt caactttact attacatttt atacatgtta aattttcaaa ttttacaaaa
    14401 ttaagttttt caaatttaat ttttttactc tcataattaa attcctcttc gtaactatct
    14461 gatataaaaa acatttttgg taaaattata cctattttat ctgttttctc atatactttt
    14521 ggaatatatt ttatgatata ttttggaaat ttactttctt gaagattttt tgctgaatca
    14581 taggaagatg acattgtaat ataaataatg ccagtaagaa ccattcctat tataaatcca
    14641 gtaaggaccc caaatattct atcaactata tgttttatat caattctctt tcttaaaagt
    14701 tcaaaaagga taattatagc aaaagtaaga ataagaactg ataataaaaa gcaggttaat
    14761 tcaatagcat ttcctgtaat tccaaaattt ctaaataaag ttacaggacc tctgtaaata
    14821 atagaagata gagagatacc aatacataaa gcaattatat caattaaatt tccgacaaat
    14881 cctcttataa tacctataaa aattccaatt aaaagaagaa taattgttaa aaaatcaatt
    14941 cctgtaaaca taaaaaaatt ataacatata atggtttatt aaagaagaaa ggggagagga
    15001 aagaaaaatt cctctcccca ttttagttat taattttctg cttttttcct tttctttttt
    15061 atttcttctg gtttttgtct tttttcttct tgttgttcct taattgtatt gagtttttct
    15121 tgaagttttt tgtactctgt atcatttgca agtttctgct caagttttgt tctcaattgc
    15181 ttgtgaagtt ccgttatctg attttgaagt tgttgtagtt cagggtcatt tttaattgct
    15241 tcttcttcaa tttttctcat tttaattcta attcccattg cctgccttaa attttcattt
    15301 ggcattggtc tcggtgggtt tcctgctatt tcttctcttg gaactttttt aggtttttct
    15361 tctgctttaa gatatgcagg agtacttaaa agaaaaaata ttccagtcag aactataaat
    15421 agttttttca tttttttcac ctcctttgtt ttttcatttt gacgattgac ccatataaaa
    15481 gtttagggta ttatcagaca ttatgaaaca aatatctatt tttgaccttg atggaacttt
    15541 aatagatgcc tattctttaa ttcatatatc tttgaatttt acaagaagaa aacttggata
    15601 taatgaactt gattatcatt tagtaaagaa aagtgttgga ggtggagata ttggattgat
    15661 aaagaaattt tttaaagaag aagatagaga taatgcttta aaaacatata gagaaacaca
    15721 aaaacaatat ataaataaag aaaatgttaa attgcaaaaa ggagcaaagg aaattctttt
    15781 attgttaaaa gaaaaaaaaa taaaaataac aatcgctact aatagaaata aatttgctgc
    15841 aaatttagtt ttagagaaac ttaacataac caattatttt gatttggttt ttactgctga
    15901 tgatgtaaaa gaaaagaaac cttctcctga aataattgaa aaaatttacg aaaaatttaa
    15961 ttgcagtaaa gaggaatgtt tttatgtcgg agatatggat attgatttga agacaggact
    16021 gaatgctgga gttgatacat atattgtttt aactggttct tctacacttg atgaaatttt
    16081 aaaagttgaa aaagaagcaa atgttttttc tgatttaatt gaattaaaaa attttctttt
    16141 aaataatcat ttgatttaac ttttaaatta ttggataata aatatggaga tagtgtcaaa
    16201 aatagataaa aaaaagatga aagagaaatc catccttacc tttctttttt aaaggaagga
    16261 ggttcttctc cctttattaa agcatctgtc cgtcgtgctt tgtggcggag gagatagaga
    16321 gggattttat aaagaggggt taggctcacc ctctggacta cgcccagagg gggagattta
    16381 aaaggggaga ggttgttaaa aaaggaagag aagcaaaaag aggggggata taaagacata
    16441 taaaaacctt aaaagacaaa gaaaggcatt ataaaagctc ttagctaatt ttggcagtac
    16501 caataagaaa aggaggagaa acaaatggaa atagtaaaaa ttcatggtag acagattctt
    16561 gattcaagag gaaatccaac aatagaggtt gaagtagttc ttgaagatgg aacagttggg
    16621 agagcagcag ttccttctgg tgcttcaact ggtgaaagag aagcacttga actaagagat
    16681 ggcggaaaag aatttcttgg aaaaggcgtt tcaaaggcag taaaaaatgt aaatgaagtt
    16741 attgcaccaa aaattgaagg tatgtgtgta tttcatcaga gagaaataga taaaacacta
    16801 attgaacttg atgggacaga aaataaatca aaattaggag caaatgcaat tttaggagta
    16861 agtttagcag ttgcaaaggc atcagcagaa tgtttaggag tccctttatt cttttattta
    16921 ggtggtgttg ttgcaaatac catgccagtt ccactttgta atgttataaa tggtggaatg
    16981 catgcagata ataatttaga tatccaggaa tttatgatat ctccaattgg tgcagatagt
    17041 tttagtgatg gttatagaat ggttgcagaa acatttcagt accttaaaaa gatattaaaa
    17101 gaaaaaaatt attcaaccgg tgttggtgat gaaggtggat ttgcacctga tttggataaa
    17161 aatgaagagg gaatagaatt aatacttaaa agtattgaaa aaggtggata taaaccagga
    17221 gaagatatat atatagcact tgatgttgct gcttcttcac tttatgaaaa cggtaaatat
    17281 aactttgaag ggaaaaaagt tgactctgaa tatcttattt cattttatga gaaaattatc
    17341 agtaaatacc caatttattc aatagaagac ggtcttgctg aaaacgactg ggatggttgg
    17401 gcaattttaa ccgaaaaatt aggaagtaaa atacaattag ttggagatga catttttgtt
    17461 acaaacccaa aaatatttaa aaagggaatt gaaaagaaaa ttgggaatgc tattttaata
    17521 aaagtaaatc aaatagggac gcttactgaa acattacaaa caattgagat ggcaaagaga
    17581 aatgggtata aaacaataat ttcgcataga agtggcgaaa cagaagattc atttattgcg
    17641 gaccttgctg ttggaacaaa tgctggacaa ataaaatcag ggtctttaag caggtcagaa
    17701 aggttatcca aatataatca acttttaaga attgaagaaa tacttgggaa tggtagtgaa
    17761 tattatggaa aacacctgat taaaaaatga aaaaaagaag aaaaatttat cttatattga
    17821 ttttaataac ttttgtaatt ttaagtattc caagaataac tcttcttctt tcttctataa
    17881 agaaattaca ttattatcag gatgaaattg taaaattgga aacggaaaat aagattctaa
    17941 aagaaaaaat tgaaaaaatt gaaaatgacc cttactatat tgaaaaactt ttaagagaaa
    18001 attatggttt aattaaagag ggagaaatag tatatagaat taaggagtga gaatgtatag
    18061 tttaaaattt ataagagaaa atactgatat tttgaaaaaa gcagcagagg caaaaagaga
    18121 aaaagttaat tgggaaaaat ttgaagaact tgacaatgaa agaaaaagaa taatatcttt
    18181 tgtagaggag agaaaaagat ggcaaaacga tataactgaa aaaatcagta caagaaaaga
    18241 tgataagaac agtattgagc cattgataaa tcaggcaaga aaatattcag atgaaataag
    18301 ggaaagtgaa aaaaaattga gagatattga agaagcacta aataaaattg ttgctacaat
    18361 accaaatatt ccgcatgttt ctgttccaga agggaaaggg gagaaagata atgttgtaat
    18421 taaacaatgg ggtgatataa aagagaaaga agttttacca cactgggaaa taggagaaaa
    18481 actaaacatt cttgattttc aacgaggagc aaaaattaca ggaagttttt tccctgtttt
    18541 taaaaatgaa ggggctcttc ttgttcgtgg aatgattaac tttatgattg atgtgcataa
    18601 aaaaagcggg tttaaagaag tgtgggttcc tgtacttgtc aatagagata gtttgttcaa
    18661 tacaggacaa cttccaaaat tagaagaaga tatgtattat ataaaaagtg atgacctatt
    18721 tttaattcct actgccgaag ttccagttac aaatctacat aaaaatgaga caatacccga
    18781 agaccaattg ccaaaatatt atgtatccta tactccatgt tttagaagag aagcaggggc
    18841 ttatggaaga gaaacaaagg gacttataag ggttcatcaa tttgataaag ttgaacttgt
    18901 aaaattcgta aaaccagaaa attcttatga agaattggaa acattacttg ctgaagcaga
    18961 aaaaatagtt caacttcttg gtttaccata tagagttata aaattatgta caggagaatt
    19021 aagtttttct gcaagtaaat gttatgatat agaaatatgg gcacctggaa taaaaaaata
    19081 tcttgaagtg tcttcatgtt caaattttga gggttttcag gcaagaagag gtaatataaa
    19141 atataaaaga agagatggaa aaaccgatta tgtccataca ttaaatgggt caggcattgc
    19201 atttcctaga acaataatag gtatacttga aaattatcag acagaaaaag gagaagttat
    19261 aattccagaa gtattaagac cttatgttgg aataaacaaa attccctgtg gataaaacag
    19321 aaaaggagaa atattaaaat ggaagattta aaaaacaaag ttaaagaaat tgaaaaagat
    19381 attattagtt ggtatagata ttttcaccag tatcctgaaa ttggatttca ggaattcaga
    19441 acatctgaaa aaatacaatc tcttttaaaa aaatttaaaa ttccttacca ggtaaaagcc
    19501 aaaacaggag ttgttggcta tttaaaagga aaaggtaaaa aaacaattgg aataagaagt
    19561 gatattgatg ctttacccgt agaagaaaaa acatcacttc cctataaatc aaaaaataaa
    19621 ggttatatgc atgcctgtgg tcatgatgga catatagcaa ttttacttgg tattgcaaaa
    19681 gttctatctg aaatggaaga tgatttagga ataaatttta aatttatttt ccagccaagt
    19741 gaagaagcac ctccaagtgg tgcaaaacaa atgatagaag aaggtgttat tgatgatgtg
    19801 aacttgataa ttggttatca ccttttttct tttctcccat ttaaaaaatt atggattgga
    19861 aaaggtcctg ttatggcaaa tgccgattct tttaaaataa aaataaatgg aaaaggggga
    19921 catggttctc gtccacattt aacaaatgac ccaattacct gtgctggtta tctcattact
    19981 tctttacaga caattgttag tagaaaaata gaccctgttg ttccctgtgt tgtttcgttt
    20041 ggaaaaattt caggtgggtt tgtttttaat gtaattccag atgaagttga aattatagga
    20101 actgtaagaa cattatcaga agatattagg gataaaataa aggacgaaat agaaaaaata
    20161 acagaaaaaa tttgttcttt atttggctgt aaatacgaag ttgaatataa caaacattcc
    20221 cctgttaatg taaatgatga atctctctct gagaaaataa taaaaattac acaaaaatat
    20281 tatccagaaa gtttatgtga atttcatcca ataatggggg gagaggactt tgcattttat
    20341 tcacaaaaaa taccttcttg ttatatgttt atagggatgg gagaaaaatg tggtcaaaat
    20401 cataactcat catttaaaat agatgagaga attttgcctt ttgcagttga atatttaacc
    20461 acaatattga taaacctaaa taacccctgc tgttaaggaa tagtaatatc cttttttttc
    20521 aatcaattcc ttatgtgttc cctgttcaac tacttgccca ttttcaacaa caataattat
    20581 atctgcattt ttaattgtgc ttaatctgtg agcaattatt atgcatgttt taccttttat
    20641 aagatttgtt attgctcttt gaacatattt ttcggttgtt gggtcaacat tactcattgc
    20701 ttcgtcaaga attaaaattt ttggctctga aagaatagtc cttgcaattg ctaacaattg
    20761 tctctgtcct tgacttaata aacttctttc ctctgatata attgtttcat atccagaagg
    20821 taaagaaaga ataaactttt ctgcattaga aattttagca gcattttcta tttcttcatc
    20881 atttgcagtt ggttttccat atcttatatt ttcctttatt gtttctgaaa atatatatgc
    20941 atcttgaaga acaacagcaa gcatatttct taaacaactt ttttttattt ttcttatatc
    21001 aattccatca agtaaaattt gtcctttatc aacttcatag aatcttgcta aaagatttac
    21061 tattgttgtt tttcctgctc ctgttggtcc aacaagagca gcaacttctc ccggatttat
    21121 atggaaattt acatttttaa gaactaaaat atctttaaca tatgaaaaat aaacattttt
    21181 aaattcaatt tctcctttta catttaaaag ttcaatttct ccatcttcaa tttcttcctc
    21241 tgtctcaaga atttctttta ctcttgtccc actggcaagt gctatttgaa tctggttaaa
    21301 ctcttctgca agtgaattta atggtctgga aaactgtctt gcatatgtta taaaaactgc
    21361 tattgaacca atagttatga gattttttat agcaaaatat ccacctattc ctccaattat
    21421 tacaaggttg atattattta taaaattcat aaaaggtcca actatcccag catatatttg
    21481 tgcttttatt cctatttttt taaaatcatt attaaaatca ctgaactttt caatttcttt
    21541 ttcttctctg ttaaatgctt ttataacttc caatccaggt atatcttctt ctattacaga
    21601 attaattttt ccaagtaata tctggttctg ataaaaataa tttctggctt ttcttcctat
    21661 aattcttgtt gtgaataaag taaatggaat aaaaattaaa attaccaatg ttaaaataac
    21721 atttattctc agcataacaa taaatatccc acaaaaaata aataagctca taagtatatg
    21781 gataatgcta ttactgagaa tattacttat agaatcaaca tcatttgtaa tcctactaac
    21841 tatatctccg tgtggtcttg tgtctataac ttctaatggt attgtctgaa ttttactgaa
    21901 taagtccttc cttatactat atacaatact ctgtgatgtt ttcagcataa aaaagccatg
    21961 aaaaaaactt atagcagagt ttaaaccata aatagtaaga cagattataa taaaatttaa
    22021 aaaaccatta aaattctttg gcataatata actatcaatt gcttttccaa taagataagg
    22081 gctaataatt tcaacagatg aagtcaacag aacaaggaaa aatacaagta aaaatagaaa
    22141 tttatatggt ttaatatatc taataaataa ttttattatt ttttttgcat ctttcatctc
    22201 tactttttca tactttctat atccatgatg atgaaaacca ttaggcattt ttaatttcct
    22261 ccctacctat ttgtatttca gccatctctc tataaaattg actcatttta agaagttgag
    22321 tatgatttcc ctgatttata attttccctt cgtctattac aaaaattcta tcacttttta
    22381 taagtgaact tattctttgg gatataatta taaaagtagt tttacttaaa tattctttta
    22441 taccctgtag tattttttgt tcatttatca tatcaattgc acttgtagca ccatcaagaa
    22501 taagaattaa tggttttttt ataactgctc ttgcaattgt aattctttgt ttctgtcctc
    22561 ctgaaagatt tactcctctt gcccctataa atgtttcata tccatcagga aaatttctca
    22621 caaattcatc tactctgcat atttccgata cctttataag ttctttttca tcaacttttt
    22681 catttcccca taaaatattt tcttttattg ttccagaaaa aagagataca ttttgaaaaa
    22741 caactgaaat tttttccctt atcttattgg atggaatttc ctgtatgtta actccatcaa
    22801 ttaaaatttc tcctttttta ggagaataaa acttcaaaag taaatttatt atggttgatt
    22861 ttccagaact tgttgttcct gtaattccta tggtctcttc attttttata tgaaaagaaa
    22921 tatcttttaa aacttctttc ccattataac taaataaaac atttttaaaa ataatatcac
    22981 ctctttttaa gtcattccca ttttttatat tttcttcttt ttctttaatt tctaatatct
    23041 catttactct ttctgcagaa gcagatgccc ttgatataaa agttaaaata tttgctatca
    23101 tcaataatga ccttaaaatt ctcattaaat agtttatata ggcaattatt tctcctacct
    23161 gcattgcatt acttcttaca agaatcccgc caaaccataa cacaccaaca ataccgtaat
    23221 taagaatcag cataaatagt ggtcctgctg ccaatgaata aaaaaatgat tgtctgatag
    23281 tttccactaa ctcattattt gttctgtcaa atcttccttc ttcatagtcc tttctcataa
    23341 aaattcttac caaccttata ccagaaagat tttcaattat tactctgttt aattcatcta
    23401 ttttactctg gacttttcta aaaagcggga aactttttct aattacctta aaatatatga
    23461 gcattgttgc tggtataaga aacaaaaata taagtgaaag tttaggattt agataaatag
    23521 ccataactaa tccaccaaag aaaaggagag gagccctgat aacaattctt aataacatca
    23581 taataagttg ttgtaattgc gaaacatcat ttgtaagacg cgtaattaat gatgaagttg
    23641 gtaatttatt taacgaatgt ataggagaat aagcaatttt tttgaaaagt tcttctctta
    23701 aatcacaccc aaaattttga ctggcaaaag atgcatatat tgtacataaa attccgccaa
    23761 ccaatccaat tagtgctata aaaaacatta aaatagatgt ttttaaaatc aaatcaacat
    23821 ttccctttat aactccctca tcaacaagtt tcgcaagaag agtaggttga taaaggtcac
    23881 agacaacttc taatatcata aaaagaggtg ctaaaataac gaatttttta tacggcttta
    23941 agaatttagt caattttttc attttaataa gaatttttaa aaagtggttt ttattttacc
    24001 attttactaa tttcttttaa aatataaaat agattaaaat tatttttaaa tattatttta
    24061 aataatcagt ttcaaatgat cctttggata acacagattg tctgaaaggt agaaaggagt
    24121 gtagcaagga cataaactaa aaaggtgaaa tcctcactac ctccttttat taagaggaaa
    24181 acttgctcct tcctttaaaa aaggaagggt gggatggatt ttagaaggag aaaaaacaaa
    24241 ggtaatttcg acagtaccaa tatttttaaa ggagaaagat gaaagtactt gtaacaggcg
    24301 gtgctggttt tattggtagt catactgttg ataaacttat tgaagagggc aataatgttt
    24361 ttataataga taatttatca actggaaata aacaaaacat aaataaaaag gcaaagtttt
    24421 ttaatattga cataagagat aaaaaaattt ctgatatttt caaagaagag agatttgatg
    24481 ctatttttca ttttgcagca cagattaatg tcaggaaatc agttgaggag ccattttttg
    24541 atattgatgt aaatataaat gggacattaa atattattac aaatttttta aaatatggaa
    24601 aaggaagaaa atttattttc tcctcaacag gaggggcaat ttacggagat acaaaaacta
    24661 ttcctacttc tgaaaaaata attccttttc ctctttgtcc atatggaatt tcaaaattag
    24721 gagttgaaaa atttctccat tattattcat attttaacaa ccttgaatat gttgctttaa
    24781 gatacggaaa tgtttatggt ccaagacaaa atccttattc agaagcaggt gttattgcaa
    24841 tttttattgg taaaatttta aaggaagaaa tttgtacaat ttatggagat ggaaaccaaa
    24901 caagagattt tgtttttatt gaagatgttg ttaaagcaaa tttgatgtgc tttaagaaaa
    24961 atgttaaagg tgtttttaat gttggaacta ataaagaaac aagtgttaat aaaattttta
    25021 attgtttaaa aaaaatcagt ggaaaaaata taaaagctat atataaagat gaaaaaacag
    25081 gggatataaa aagaagttgt ttgaaaatcg ataaaataat aaaaaaagtt gggtgggaac
    25141 cagatataat gattgaaaaa ggcattgaga aaacatataa atggtttaaa ggaaattttg
    25201 aacaatgaaa tatctaataa caggcggggc tggctttatt ggttcacaca tagttgaaga
    25261 attgcttaaa agaaatagtg aaatttttgt tttagatgac ctatcaactg gttcactttt
    25321 gaatgtaaaa gattttttaa gaaatgaaaa ttttcatatt tttattgata caatactaaa
    25381 cgagcatata acagaagaac ttgttagaag gagcgatttt gtttttcatc tcgcttctgt
    25441 tgttggggta aaaagggtaa tggaaaatcc cattgattct ttaattataa atattttggg
    25501 aacacataat gttttaaaaa gttgtgtaaa atataacaaa aaggttttga taacttcaac
    25561 atcagaaatt tatggaaaga ataaaaatgt tccttttaat gaagaaacag atattgtaat
    25621 tggttctaca aaaaagaaaa gatggagtta tgcctgttca aaagcaattg atgaattcct
    25681 tgcatttgct tacaacgagg aacaaaattt atcagttata attgtaaggt tatttaatac
    25741 agttggttca agacagacag ataaatatgg aatggtaatt cctaaatttg ttaagcaggc
    25801 gttgaaaaat gaagtaatta cagtttttgg agatggaaat caaacaagat gttttatttc
    25861 tgtaaaagat gtagttgaaa cacttctaaa actgatagaa aaaaaagaag catatggaga
    25921 tgtttttaat gttggaagtg atgaagaagt aaaaattaaa gacctatctt ttaaaataaa
    25981 agaaattaca ggtagtaaat ccgaaattaa atttataagt ccagaaaaaa tttatacatc
    26041 tggttttgaa gatatggaaa gaagagttcc tgatacatca aaaataaaaa aaataattga
    26101 tttcaaaatt aaatattcat tagaagatat tattaaagat gtagttaatt attataaaaa
    26161 tattcaggca actgtattgc ctgaaaaata gcaagtggca aaatgctata aaaggcacaa
    26221 aatccaaaaa tcttagagga tgatactaat tttaggtata aagacacaga ggaaaaataa
    26281 aaaaatattt taaaaaggca ctctttttaa accctctccc cttggggaga ggatagtggg
    26341 tgaggggaaa atatctattt aatccgccac aaagcgtagc ggacgctaaa atacgcgctt
    26401 tgtgcctatg tggttcactg aaatattaag tgaatattaa ctttgcagaa aaaaacaaat
    26461 gaatagaaaa aaagaaaata ctcttctttt tttaatactt ttttcaggat ttattataag
    26521 attagtttat ctttattttt tcaaagatac tgcattcttt aatccatact tgatggataa
    26581 gcatgaccag aagacattta tattatgggc tgaaacaatt ttaaaacatc cattttatgt
    26641 tgatggaaaa gtattttata tggcaccatt ttatccatat ttactttccc ttttttacct
    26701 tttaagcagt ggaaatatcc tgattatttt aatttttcaa gcagttattg atacaggaac
    26761 atgttatctt ttatatctaa taggtaaaaa actttttgga gaaaaagttg gtcttatttc
    26821 atcttttctt gggtgttttt acaaaacatt tattgtttat tctatctcaa ttttaagcga
    26881 tggaattata ctatttcttt acactctttt tttcttttta cttatatggt cccttgaaaa
    26941 accgacattt aaaagatgga taattacagg aattattttg ggcttttgtg ctttatctaa
    27001 accaacaata ggaatttatc tccctttttt attttttgga ctattctatt taaaggataa
    27061 aaaactatta ccactgaact tatctcatca aaagcaagca cttattactt tttttcttat
    27121 tatttttgtt tcaggtatta caattttacc agttactata agaaatttta ttgttgaaca
    27181 taagtttgtt ccaatatgtt ttaatggtcc tataaattgg gcaattggta atagttctga
    27241 ttcaatagga cttttttatt atccaaaggg agaggtttta agtgtgtttt cattgccttt
    27301 ctggaaatta ttcttaacaa aattatacct attttttaca agttatgaat ggccacaaaa
    27361 tatgaatgtg taccttgtag aaaaagttat tccattttta aagctagctt ttataaaatt
    27421 tggatttgtt gttcctgttg gtgtctttgc cttttttata ctttttaaga actttaagaa
    27481 atattacttt ttaattacat ttacattttt taatgttcta tgggtggttc tttttttcat
    27541 tacagataga tatagattgc ctgctgtaag ttgttttatt gttttatgtt cttatttttt
    27601 tatctgggtt tatgaaaaaa tctttagaga aaaaaaattg tttttaccat ttctaacctg
    27661 tttttttatt ttaatttttg cttatttttt tgatataaca ccaggaccac tcattccaga
    27721 tgaaagtaaa aaaatttttg cacttttaag tgtaaaaaat atacagaacg atttaaaaaa
    27781 tggaaatatt aaaaaagcaa aaaaagaaag taaaaaatat aatgaaattt tgcctgatga
    27841 ccctaaaagc agttatctac ttgcttgtgt ttattatgaa ctaaaagata ttgataaatg
    27901 tttttattat ttagaaaaaa cacttgaaat aagtcctgat tacgaaccag caaggaaatt
    27961 tttaaatgat atcctaaaat gataacatta tttaagacaa aataaacaaa tggaaaatgt
    28021 aatctgcccg gtttgtaaaa aaaatgaagt agaaatggaa gaaaaatatg gagaatttac
    28081 tttatataaa tgtagaattt gtactcttga atttttttat cctttatctt ataaaagttc
    28141 cttgtatgag gaattttatt cttttggata tggcaataca gtcataaaaa ataaacaaaa
    28201 atttccgata ttattccctt atgtgctctt taattatatt agatttttta ataagttttc
    28261 agatacaagt ttattagtta tagcgagatg aaaaaagaaa aaatctgtat tatttgtaat
    28321 gacccattct cagattatct tttgaataga ggaaaagagt gcatagaaaa atactttaat
    28381 cctttgctta aatttgaaac acatattatt tcatttgagg attgttataa atatcaaacc
    28441 agaaaaatcg gtactttaat tattcatcct ttttgtgaaa aacaattcaa aaaaacatta
    28501 ttttacccct ggagagttat tagaacatta tcaagacatc ttttgagagg tataaaaatt
    28561 ataaaaatta taaaaataga gaaaattcaa cttgtaaggg ggtatggtat ttctaattta
    28621 gcttttattg gtcttattgc atctaaaatt acaaaaaaac cttatattat ttctttgcat
    28681 aacgattatg attatatgtt taaactgaaa ggaaaaacaa aaagaataat gtggcatttt
    28741 cttgaaaaac tcacaataaa gttttctgat tttgtaattg tagtaagtcc ttttcttatt
    28801 gaatatgcta aaaaacatgg aaataaaaaa gttgtttata taccaaaccc tcttgatcta
    28861 gaaaaattta ttgaagttgg aaagaaaaga acttttaacg aaaaggaaga aaaaacattg
    28921 atttttgctg gaagattttt tgacccacaa aaaaattttt atcgtctttt agttgcatat
    28981 agtaaaataa agtcagaact aagggaaaaa agtaaattat ttgttcttgg agattatggg
    29041 ggaaaactcg gatattataa ggagttaaca aaaaaattgg gaatagaaaa aaatgttgag
    29101 tttattggaa gagttccaag aacagaaatg ccattatggt atatgaaagg tgattttttg
    29161 cttttaccct ctttatatga agcattacca aatgcactaa ttgaagggat ggcaacagga
    29221 cttcctgttt taacttcaaa gcatccttcc tgtacttatc ttgttgatga gagtaatggg
    29281 gttattgtta atccattgaa tgtagacgaa ataaaagaag ggattgaaaa attattaagt
    29341 atgaagcaag aagaatataa agaaaaagca gaaaattcag tcaaaaaggt taaaaatttt
    29401 aatgatgaag aagtttttaa aaaagttgcc tttttatacg aaaaattaat caagaggtaa
    29461 aaatgggtag catttgtgga atttataatt ttgatgaaaa aaaaatagta gataaaaaaa
    29521 tacttaaaaa aatgtctgat aaattaaaac atagagggac tgataaagaa ggatactatt
    29581 taaatagaaa cataggtttt gggcacaaac aatttatgat tggatacgaa agtagagaac
    29641 aaccaataag taatgaaaat gaaacaattt ggattgtttt agatggagaa atatataact
    29701 atgttgaact aagggaattt ttgattaaaa aagggcataa attttcaaca aaaagtgatg
    29761 aagaagtgat aattcatctt tatgaggatt atgaaggaga atgtctacaa tttttaagag
    29821 gtatgtttgt aattgctata tgggatgaaa gaaaaaaaag attatttatt gcaactgata
    29881 aaataggaca aaaaacatta tattatatca ataaggatgg aaaatttgtt tttgcttctg
    29941 aaataaaagc acttcttgaa tttcccgaat atgaaaaaga aataaatttt gaagggataa
    30001 attatttatt tgcctatgaa tggtggattc cttggcccta tacttctttt aaaaatattt
    30061 ataaaatgct ccctgcaact tactttatga ttgaagggaa caaattaaca gaaaagaaat
    30121 attggactcc tgattttata aataaaacaa aaaagaagga aaaagaaatt attgatgaac
    30181 tttataactt aattgatgaa tctgttaaaa taagattgag aaataatata ccagttggag
    30241 cattactttc tgggggagtt gattcaagtg gtattgttgc ttttatgagt aattaccttt
    30301 cacatattaa aacttttaca attggattta aaaataaaga aaaactaaat caggaatttt
    30361 taagagcaag agaaatttcc gaaatgttta ggacggaaca ttatgaagtt ttgttaacag
    30421 atgaagattt aattgaaaat attccacaac tggtaataaa ccatgatgaa ccttatgttt
    30481 gtataactgg gttatataca tattttctta caaaatttat aaaagagaag ggtatatcat
    30541 taactttaag tggaaacggg ccagatgaga ttttctgggg atattcaagc ggagttgatt
    30601 tgaaaataaa aagtattgtt aatgggattg ctaaatatgt tcctgatgaa atcttgaata
    30661 tatttcctaa atttcttctc cctaaaaata ttcaaataag aatagaaaac ttaaagaaac
    30721 cgttttccat taagttatcc gaaggaataa acaaaaagtt tgaagaaaat acttttggac
    30781 tattcaagaa aaattttcct aaaattaata aaaactatgg attgcttgct aaagaatatt
    30841 tttctgaagc agaaggaaaa actgatttaa taaccgctta tagttatact gctttaatgc
    30901 taacttatgc ctttgggtta acaattcagc ccaatcttga tggaatggca aatacagtag
    30961 aaatcaaatc tcctttttta gaccagaaaa taattgaatt tgccttttcc cttccattag
    31021 aatataaagt taaagggttt acgccaaaag gatgtaagta tattttcaaa aaaacaatgg
    31081 aaaaaaaatt gccaaagaat ttattatatg caaagaaact tgcttttggg acagacatta
    31141 aatgggaaaa attaattgtt gaaaaatttg gtgaaaaaat aaaagcagag gttctaaatg
    31201 gatggtgggt aaaaaacaat tttttttcaa gagaatacat tgaaatgcta ttttctaaaa
    31261 tgccagagac aaaaacatca ttacaaatct tgcgtcttta tacacttgaa ttatggtata
    31321 gaatctattt taaaaaatga gaaaagaata tttattaaaa tttaaagacc aaatagaaat
    31381 ctgggatgaa aaatggaata ctgctgaaat agaaaaagaa atttatgaag caaaaaattc
    31441 tcccattgct gatattataa aaattttctt accttctcaa ggaaaaattc ttgatgttgg
    31501 ttgtggatta ggtggatatt taatatggct aaaaagcgag ggatttaata tatatggaat
    31561 tgacttttca aaggaagcaa tcaggcggtt aaaaatttat aatccatctt tggatgttga
    31621 aatatctgat tgcgaagcat taccattctc agataattat tttgatgtat atatctctct
    31681 tggtgtatta gaacatatag aatcaggttg tttaaaagca ctgaaagaag caaatagagt
    31741 tttaagggat aatggaatag ttattataag tgttcccttt ttaaatttat ataggttgat
    31801 aaaagatttt tttattttta aattaatggg aaaagaaagt tatgttttaa ctaatgtaga
    31861 agaaaaaaat atagtgtttt acaggagaaa actttataaa gaggagaaaa aatatagttt
    31921 ttttcaatat tatttttctt tatcagaaat aagaaaactc ctcaaaaaat ctcaatttaa
    31981 tattattctc tggactcctt attcagtttt acccggtatt ttagaaataa aattatttca
    32041 aaaattatat aaaaagttta ttttaagtaa aagtaaagaa aacctgtata aaataaaaag
    32101 aatagaaaat tcaaaaaaaa ttaataataa atcaataaaa aaaagaaaat tttattttat
    32161 tcttgctaaa cgattttata aacttctctc atgggaaaag agcaaaaatt tattagaaaa
    32221 aaatatattg aatatcttta gacatatttt tggacacatg attctcatta ttggggtaaa
    32281 aaatgataac taaaagagaa aacaaatata ttaaatcgcg tttgtcacta ccatggatag
    32341 aatatataaa aatttataca tccctaaaag ggaaaaaaat tttagatgta ggatgctgga
    32401 ataaatggtt atttatataa ttcaggaaat atagaagaat ttatagaaaa agtggaattt
    32461 tttataaaaa ataataattt aagagaaaaa attggaaaag ccaataaaga aaaaattatt
    32521 aagaattata actggataga aagtgcaaaa aaattaaaaa ctgtttacga gaaaattcta
    32581 tgagagaatc aataaaagtt gcaaaatcaa gtgcaaaact cgtatctgct gaattcataa
    32641 atatcatatt tggtattttc tattttttaa ttattacaag aactttttca aaaattgaga
    32701 tggctactat tgcaattatg ggagttataa caaatatctc tgctgtcatt attggcttcg
    32761 ggatgcatgc aacaagtgta aaattgatac ctgaacattt atcaaaagaa gaaaatgaaa
    32821 atgtttctga aatcttacaa attgcttata tattggtaat ttttgcttct attttttttg
    32881 ttttttttac ttttcttttt tcatctcaaa tttcaagcat tttttttaaa agtccatcct
    32941 attctaattt aataaaaata atctcgtttt caatttttac caataaatta tatgaaacat
    33001 cttttcataa tttaactgca tttcaaaagt ttacagaaat aagtttactt caaataatga
    33061 acaatgtttt tgttagaata tatgctcttt ttctctattt ttcaagtgga attgaaggat
    33121 atttaatagg acttataaca ggacaattta taatttcaat ttgtatgata tttttaacaa
    33181 aacaatacct atttaaaaaa attaaagaac acaatttcaa aaaaattctt aaattttcat
    33241 ttccttttta tctaaatgga tatattggat atggagcaaa tcatgctgat tctcttatag
    33301 taagcatttt tctttcccct tccttaattg ctacttatta tgttgtaagg agatttattg
    33361 actatcttga aatttttatt acttctattc ttactccttt atctcctaaa atggttgaaa
    33421 tgaaaaaaaa tggaaaagaa acattagaaa aaattttcta taaatcaaca aggtatttat
    33481 cttttatttt aataccaact tgccttttta tgatttctat ttcctatttc ctattggata
    33541 tttatgggaa aggaaaatat ttaagtggat tacctatttt aataattctt agtttaggta
    33601 tgatttttaa tggtttttat ctacttttga gaggaactat ttttgtttgt agtaagccaa
    33661 ttgaaactac aaaagtagaa ttgtttaagg cgtgtataaa tattttactt ccgttaactt
    33721 taataagaaa atttaatatt cttggaatta gttgtggtaa atttatttct ttatttactt
    33781 ctgttttttt cgcatattat attggtaaaa aattattaat attaaaattc gattgggaaa
    33841 atataaaaaa atctttttta atttcttttt tctccgtcat tttaatcgta attttacaaa
    33901 ttttatttta cactccattt tggatgatat tatatatttg tgtaggaata acagttttct
    33961 cccttttatt ttttaaattc actacacaag atgagaaact tttttttaaa gagatacttt
    34021 tgaaatggaa caattagtaa gtattatact cccagtttac aatggagaga gatttataga
    34081 aagagcaatc agaagtgtat tagcacagac atataaatat tgggagttga tagttattga
    34141 tgacggttct actgataaaa caagtgaaat tgtcaaaaga tatttaaatg aaaaagtaaa
    34201 atatctttat cagaaaaatt caggtgtgag tatagcaaga aataaaggta ttgaaatatc
    34261 taatggcgat tatattgctt ttttagactg cgatgatgta tggaaacctg aaaaattaca
    34321 agttcaatta tcaaaattta tggaaaatta taatgttgga cttatttata cagatgtttt
    34381 tatagttact gaaaggggta aaattctcgg gaagttttct caatttaatt atccttactc
    34441 taataaagtt actaaaaaat tatttttgca aaattttata tgtacatcat cggtgataat
    34501 gaataaagaa atattaaaaa aaacaggact ttttgaccca gatattaaaa taggtgagga
    34561 ttatgatttg ttttttagat taagtttata tacaaatttt tattatgttg atagtccact
    34621 attatactat caaatatcgg aaaaaggtct atcaagaaac aaaataaaaa tgtgggaaga
    34681 aaatattaaa attttagata aatggttaaa gaaatatcca gaacttacca aaattcttgg
    34741 gactaaaaca ataaaagaga agaaatcaaa tctttttatt ttgttaggaa aagaatattt
    34801 tgaaaatggg aaatttaatc aggcacaaaa aacgttttat aaagcaatta catataagaa
    34861 aaatataaaa ggaatatctt tatttttact ttctttttta ggtaaatttg gtaagttttt
    34921 actttcttta aaacagaggg ttttaaaatg atttatttta ttattagaga tttttgggga
    34981 aaagggggaa ctgaaaaggt tgcacaaact cttgcagaaa aattttataa aaaagaaaag
    35041 aaaattaaaa ttattacttc tggcaagagg atatttagaa aaaaaatatg gaatgagatt
    35101 attgttattg aattattttc acctaacata aaatatatag gaacgctttt ttattatttc
    35161 ttccttacta cttatctatt tttgacaatg agacaatata aagtaatttt agtatttttc
    35221 cttaaacaca gcgcttttat ttcaattttt atttcaaaaa tattgggaaa aaaagtaata
    35281 tgtcggattg ctggtacagg aaaatatgga gatattcaaa cactcaaaaa aattaaattt
    35341 catcaaattc tcctatatat attgagaaaa gcagataaat acattacact aactgaatgt
    35401 atgaaaaaag aactattaga agaaaagttt gatcctaaaa aaatagaaat tatcccaaat
    35461 ggagtagata ttaaaaaatt tagacctcct gataataaag aaattcttaa agaaaaaatt
    35521 ggtataaaaa agaaaaaaat tctggtattt ataggacgac tttcagaaga aaaaggatta
    35581 aaaattttac ttaaagcaat taaaaatgat atttttaaaa atacacttct ttatattgtt
    35641 ggtaaaggat atttggaaac agaacttaat gaatttgtaa aaacgaattg tattactgac
    35701 agagtagttt ttactggttt taaacaagat gtagtccctt ttttacaagt tgcagatgtt
    35761 tttgttcttc cttctatttc agaagggttt tcaaatgcac tgcttgagag tatggcaaca
    35821 ggacttccag ttgttgcaac aaaggtaagt ggcaatattg atataattga aaatggtata
    35881 aatggatttt tagtagaacc agaaaattca gaagaattag caaaggcact tgaaaaaatt
    35941 ttaaaagatg aaaacttggc aagaaaaatg ggggaagaga acagaagaaa aatagttgaa
    36001 aattattcta ttgataaaat tgttgagaaa tacattgctc tttataataa cttaatatca
    36061 aaaagtataa ttaaacaaaa ttaaaagaga agggttttaa aaatcttttg ggagaaaatg
    36121 tttatttgaa tttttgtttc taaggatgta taattaacaa aaatgttaat tttatatacc
    36181 gaaaatgaaa tatataaaga ttagagaact taaagaaaaa atttatttta caacaaacga
    36241 aatcgctgaa tatcttggaa taaaaaaaga atcggcaaga gttctttgtt caagatattc
    36301 taaaaaggga ctaattataa gattgaaaag ggatttatac attttaagag aaaaatggga
    36361 aactctttct attgaagaat attttaatat tgcaaatata ttccaagttc catcttatat
    36421 ttctttaatg actggtttat attatcatca gataactaca caggtccaaa gaaggtttta
    36481 tgaaagtata tctttgaaga gaagtatatg ttttagtata gagcaaactg aatttcatta
    36541 ttacaagata aaccgaaaat tttattttgg attttttaaa aaagataaaa tttttattgc
    36601 agaaaaagaa aaggcatttg ttgatattct ttaccttact tctcttggaa aatataagtt
    36661 tgatgtttca tcagtcgatt taagaaaact taaaaaaaat aaaattgaaa aaattcttaa
    36721 aatatatcca gaaaaaacaa ataaaatatt tataaaaata tggaagagtt aatacagcaa
    36781 gaaaaatttg aaatagaagt acttgaatat ctgaatagta aaaagtattt agataaaatg
    36841 atatttgtag gtgaaacaat gttaagattg tgttatggat tgaatagatt ttcagttgat
    36901 cttgatttct ggataattaa aaaaataaaa tttgataaat tttttttgga cttgaaaaat
    36961 tttctagaag aaaaatatat tattaaagat tgtgccaata aattctccac aattcttttt
    37021 gaaattagtt cttccgaata tcatagaaat ttaaaaattg aaattagaaa agaaattaaa
    37081 aaaattaaat atgaaaaagc aattgcattt tcaaaatttt ctaatattca ggttcttcta
    37141 aattcaatag acctcaatga tatgatgaaa tttaaaatag aaagtttttt aagcaggaaa
    37201 gaaataagag attgttttga tatagaattt ctttttaaga gaggaattcc aattaactta
    37261 aatgtaaagc aagcaaaatt acttctaaaa gaaatagaaa aattgaaaga aaatgattat
    37321 aaaataaaac ttgggtcgat tattgaaaga gaggtaagaa attattattt gaaaaaaaat
    37381 ttttatattt taaagatgga attgatgaaa atccttatta cttaaattat aaaaatgaaa
    37441 aaaatatgtt atgttatagg aactctgaaa gttggaggag cagaaaaaca actttattac
    37501 cttgttaaga atcttgataa aactatgttt tctcctgttg ttattgcttt aagaggagga
    37561 attttagaat atgattttaa agaaatatgt tcattaaaaa ttgttggtaa aaggtggaaa
    37621 atagaccccc ttttcttttt aaaacttgtg cctattttga aaaaagaaaa agtagatatt
    37681 ttacacactt ttatgttcac tgcaaataca tgggggcgcc ttgctggaat tatttgtaaa
    37741 atccctgtta aaattgtttc tgaaagaagt attgacctat ggaaaaagag ttatcatttt
    37801 ttaattgata aaatacttgg aaaatttaca gataaaatta tatgcgtttc agaagaagtg
    37861 agaaatcatt atttaaagaa aacaaaatta ccttctgata agtttattgt aattgaaaat
    37921 ggagtgaact tacaggagat agaaaaagtt aaagagaaaa aagaaattaa agaagaattc
    37981 ggaatacaag atgatgattt tgttattctt acaggaggaa gattatgtaa ggaaaagtca
    38041 attgattttt ttctttctat tgttcctgaa ttaaaaaagc aaatgactaa attaaaaatt
    38101 ttaatagtag gggagggtga agaaaaagag aaccttttta atataactaa atatttgaaa
    38161 cttgaggata atgttatttt tacagattat agaagggata ttttaagtat tattaaaatt
    38221 tctgatttag tagttttaac ttcaaaatgg gaaggtatgc caaatttaat acttgaaggt
    38281 atggcattaa aaaaggcagt tatttcaaca aatgtaggtg gatgtaaaga aataatccaa
    38341 aataatgtaa atggttttct tattgaatat ggagataaaa aacacttgat tgaaaagata
    38401 ttatttcttt ataaaaattc aggtgaaaga gaaaaaatgg gagaatctgg ttataaaatc
    38461 gtaaaagaaa aatttgattt aattgtgaaa ataaaagagt atgagaaaat atattcaagt
    38521 tttaaaatag ggaaaaatta aaaatgaaaa ataaaataat aattttttct gctattcttt
    38581 tgactttttt aatctggata ccttatattt tcccctattt ttctaatgtt gacgaaccgg
    38641 catattttct gaatgctctt tcagttttta aaggtaatgt actatatagg gatgtttttg
    38701 aaaataaagg acccatcatg actttgattt gtcttttttt tataaaaatt tcacctgaaa
    38761 attatacttt cctttttcat ttaacactgt ttttactgct ttttatagag tcaattctaa
    38821 tttataaagt tttagttaaa ttaaaactta aaaatttggc aatttatgga tttttctatc
    38881 cagtatttct ttccttgttt tatccaggaa atatgacaat aggtggtgaa ccatttataa
    38941 gtttcctttt acttattatt ttttatattt ttttactctt tcccaataag ataattccaa
    39001 tttttgttat tggcttatta tcaggtataa ttgttttatt taaaccattt ggcatttttc
    39061 ttatattatt acttatattt ttaatctatt acaaatcaaa aagtattaaa aaaatatctc
    39121 tttttgtttt tgcttcaata gttcccttat tattttttct cctttatatt tttaagtatc
    39181 atatattcca tgatttttta ctatgggcta ttaaataccc tgtttttctt tccagaacag
    39241 tacctacttt aaaagagtgc tattttattt ttcctatgtt aggaagaatc tttttattaa
    39301 ttccattttt tacagttttt gggatatacg aaattattaa aaaaaacaaa aatagagagg
    39361 aaatttatat aattcttttt ttaatattta ctgctttatg gggtgcattg catggtctcc
    39421 cttttccaca ccattataga ttattatttc cttttttatt tattatttct ttaataggat
    39481 atttcaatat tgttttttct caaaaatttc aattgaaaaa cattctcaat attttcataa
    39541 ttttctctat tcttcaagga tttttttatt ggaatggaat tgatttttac aaaaaatgga
    39601 tagaattttt aagtgagaaa aaatggacaa aagaatatga aattaaatca aacagtgaaa
    39661 ttttagaatt cttaaataaa aatacttctc ctgaagataa aatagttatt tggggacata
    39721 atcctaaatt atatcttctg tctaaaagag accctggaac aaaatttaaa gcatggtttg
    39781 acccagttat tggaattgtt ttttacgata ttcacaaaat acagcaattt ccaaatgccg
    39841 aagaattatt tatttttgat ataaaaagaa ataatccaaa atattttata gatgctacaa
    39901 aatgtagttc tattggcata ccatattatt cagttgataa atattataaa atatatgaat
    39961 ttttattaaa taattacaaa aggattaaag aattaaatgg ctatgtaata taccaaaata
    40021 aaaaaaatgt gtgcaatatg tggaataata aaaaatgaag gaaaagtaga agaaaaatta
    40081 ttatttgaaa tgacctgcct tatgaattat agagggcctg acgaggaagg atattttata
    40141 gaagaaaatg taggacttgg tcataaaaga ttgagtataa ttgacctaaa aacaggcaaa
    40201 cagccacttt ttaatgaaga taaaaaaatt gttcttatct gcaatggtga gatatataat
    40261 tttaaagaac taagagaaaa tcttgaaaaa aaagggcata aattcagaac aaattcagat
    40321 aatgaggtaa ttgttcatct ttatgaagat aggcaagaaa aatgtattga ttatttaaga
    40381 ggaatgtttg cttttgcaat ttgggatagt aatgaaaaac aattgtttct tgtaagagat
    40441 aggttaggac aaaagccact aatttatagt ttagttggaa aggatattgt tttttcatcc
    40501 gaaattaaat caatcctgtt atatccagaa ataaaaaaag aaataaattt agaagcatta
    40561 agtgattatc ttacctatca ggcaatcccc tcaccaaata caattttcaa agatataaag
    40621 aaaattcccc ctgcctgtta tttaatatgg aaaaatggaa atataaaaat tgaaaaatat
    40681 tgggatattg atttcacaaa aaaaaattat tttaaaaatg aaaacgatta tatggaatta
    40741 ttatgggaaa atttaaaaga agcaacaaaa ttaagattaa tttcagatgt tccacttggt
    40801 gcatttcttt ctggtggaat tgattcttca acaattgttg gaattatgag tgaaatatgt
    40861 gagatacctg taaaaacatt ttcagttggt tttgatgtga aagaatatag tgaattagaa
    40921 tatgcaaaaa ttgttgctaa gaaatttgga acacaacacc atgaatttat tgtaaaacca
    40981 gatattatca atatcttacc aaaacttgtc tggcattata atgaaccctt tgccgattct
    41041 tctatgattc caacttatta tg
//
LOCUS       DAOOVW010000010        37843 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_699_length_37843_cov_8.171536, whole genome shotgun sequence.
ACCESSION   DAOOVW010000010 DAOOVW010000000
VERSION     DAOOVW010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 37843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37843
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_699_length_37843_cov_8.171536"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="PLW95_02445"
     CDS             complement(<1..711)
                     /locus_tag="PLW95_02445"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HOV21525.1"
                     /translation="MDKREIEETQKQVGNWASICFTDKRLFPAPVQLKFIKQDFPVIK
                     FGELPIKIGDKCFEHGLSTHANSEILVNIPKGAKVFKSFIGVGIDDTYDNNYNSQQFI
                     ASVVGSIEGKDEELVKTPVLKKGDKPFFIEIPLSSVQQIILKVNSVSGDLSHPRADWA
                     DAQIIMEDGEVIWLDECQNNFLLLESENPPFSFIYNNIPSSSFINKWEKHIEKEEKNS
                     FIFYKISWIDPETSLLVKV"
     gene            complement(1283..3961)
                     /locus_tag="PLW95_02450"
     CDS             complement(1283..3961)
                     /locus_tag="PLW95_02450"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOV21526.1"
                     /translation="MNKKILLVFLIFCLSHLIYKSLCCQVSFERNLVEPVIMENNFLK
                     LTIDKARGGVIRSIVFKPAQRELTEEFFTGKYWAGGIAEDRLAGQGYPEGEINQAKYE
                     GELKREGDKQIFTLSYLSKSDKNKDIEFIKKYILKDNETRVAIVWEIINHRKEKISLT
                     PWVHNIVDRKLSFTCLPQLDGIKYIKPSADKFVDVVRNWIGAYSDNFFLYFTPKYCDI
                     TKYYYCYWNSYHSIEWSYNTINLAPGEKWETLYYVGIIPQVSKVGWVSPEIVSGYQWQ
                     ENNLIVELASPINLGEVEIIFTLFNKDNLEKGEILKKTKEIILAGNNPLEISLQIPEN
                     FSEGKLEIVFNQNNKEIKLGETIESEENKIIFPLKKGISFEDVSLSEWKKTRKGFITR
                     EKGYQGGYLIKKTSGISIWEVDTQEKVFKDDILTPIGEKEQLEIYGAKRERVAFQIVI
                     RNNSSENKLVKIDINPFISGKDKIGLKEIEIYKVGYVRTYTPSGLSANIPIGLYPDPL
                     ISESSFSVEPKTNIPVWVIVNIPKNINSGIYKSSLSVKIGKNQVIKTPVKLKIFDFVL
                     PEKPYLKTDFGCWFLSENLLKEIGFQGSSKNFQQLCQENYLKHRITPREKNIPWDGDE
                     NEIINALNYYKEKEITSLYIPSFLVKNEIKFKEIVRILQKTGLTDIAFVYTYDEAPAN
                     LYPKIKEFCEYLHKIEPKLKFLGTIYGDNPEYLFGHVNIWCRGLQRSEWIRKRMQTGD
                     EFWSANIGSANIEASNFNLRKEFWRIKSYGYCGCLYWNCIGGYGNDNPWKDPLVAGSN
                     GNAHFLYPTKTKPINTIRWEMVAAGINDFDYISILEDKVKKIKLDEKNNNEYLDAANL
                     LLNELFQDNFVENNITSPEILKTYRLKIGYLIENLK"
     gene            complement(4074..4922)
                     /locus_tag="PLW95_02455"
     CDS             complement(4074..4922)
                     /locus_tag="PLW95_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOV21527.1"
                     /translation="MNKKILLVFLIFCLSHLIYSEEKMINFAKEEGVSYKVSVGHYFD
                     VPQYGKKRDNDLIRYRVGIVEGNIIKDKEGKVIGEIINNKDGVIEIDFKEKKGYIKFS
                     IEKSSDKISNLGKQELNILDEKNNVIGKASIKKDGEIDKIYYIVNEDGDFEERKGLLT
                     DGRDSYAPNTGLCYVFWRVPGDDSFITIDFDLKKPRKITNVVLKGQNLSPMFGVSKSV
                     LYSSKDGIVYEIKKVIEKVEPPKTVNWNIEFTDLNINERYIRITTWALENKWLNLTEV
                     EIFGKE"
     gene            complement(4922..7048)
                     /locus_tag="PLW95_02460"
     CDS             complement(4922..7048)
                     /locus_tag="PLW95_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21528.1"
                     /translation="MRQSFKFVIYLVLFTPILLFSEANISQENLIKKQKLILKGKEQK
                     SFNFNVSNPDGYTVLWIKGRFNIERGEGWGDYGLQVSINKKSISEKKLINKKLILINS
                     AYIGQRPTNLFSEDKDAWFLRFDSDYSPYNSEVNGDPYNRISSFSNANTSWNKYEFEN
                     AYYVYAFNISDYIIKGINEISFRNINEIYSIEMSFTLEKFSEPLLFFTHVSDRIIFPC
                     SFPREEECNVKIEKVCTPGEIIPITLSLRNESEENIIFDIITTPLESPDNKFIPKENI
                     EVFVLTFSPTKSTIYEFINPTGEILHPDILNPSNKWEVKAKSSSSLWLRIKIPQDISS
                     TDWTGKVILKSGDKEIYIPIKVKILPFELSQVDTIFGLWDNKLPGAFDNLTSKRIEDL
                     KNHGINSITTDPWTSPITIKKNQDEILVDTSRLKKTLQVYKTEGLNTTGPFPYGTLEP
                     VFNQIKTISGQEIETPEYQNVVDIVLKEIMSLADELGMKLYFHPTDEPDVHPDCIENY
                     EKLLKCIKKANGKIWSNNTVAGFRKWYQLIDINCVVGGIEDALKEENYTNFFFPQWGS
                     VEPEWVKANINKMHIQTQVRSTMPYEIRKKYGIYLYYTGCKGIWGFAYNWGTSNWYIA
                     WPFLNDEGKMYSTIGWEMLREGITDYKYLKTLEDILSKKIGEKKAREKVISLLPPFTS
                     ILTISNEYFSIIREKIINEILKEKEK"
     gene            complement(7094..7768)
                     /locus_tag="PLW95_02465"
     CDS             complement(7094..7768)
                     /locus_tag="PLW95_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOV21529.1"
                     /translation="MNRGRARISASTASCGGGFTLIELLVVIAIIAILAAMLLPALSQ
                     AREKARQSVCMSNLKQIGYAFLMYCDDYDGYTPPNRVGMTSNCWFSLISRITYKPGTP
                     TTWEPLKPAIPFTTFRCPSALQTGYYDWYPFYKVGYAYNSGAANKKVSRNPTPTKTPI
                     VFDGKSLYGDGYYGYTDLGKYAVYTRHSGGMNLLFLDGHVEWIPDSYWPRTGIGTTWA
                     GFRWRW"
     gene            complement(8504..9649)
                     /gene="proS"
                     /locus_tag="PLW95_02470"
     CDS             complement(8504..9649)
                     /gene="proS"
                     /locus_tag="PLW95_02470"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014041962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOV21530.1"
                     /translation="MKWSNYFIPTLKEVPKEAESISHKLMLRGGFIDKVSSGVYTYLP
                     LGYRVLRKVENIIRQEMNKSGGIEILMPSLQPSSLWKESGRYGKMGKDMIKFIDRSER
                     EMIFGPTHEEIITDLVRRHIKSWKQLPLLLYQIQTKFRDEIRPRFGIIRAREFLMKDA
                     YSFDIDEVGLDISYEKMKEAYIRIFSRCGLKTNIRTADSGVIGGKFSEEFVAEGDLNE
                     LEVGHIFKLGTDYSRSMKANFVDRDGKEKPIVMGCYGIGVSRIIAAAIEGSYDEKGII
                     WPISIAPFKVIIIPLIAQTDVKETSEKIYQDFPNKKNVLFDDRDETAGVKLTDAELIG
                     IPIMIVLGKKLKEGKVEVRERKEKTYQEVDVENVVEYISDLVKSNCK"
     gene            10010..10083
                     /locus_tag="PLW95_02475"
     tRNA            10010..10083
                     /locus_tag="PLW95_02475"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10044..10046,aa:Asp,seq:gtc)
     gene            10672..12393
                     /gene="thrS"
                     /locus_tag="PLW95_02480"
     CDS             10672..12393
                     /gene="thrS"
                     /locus_tag="PLW95_02480"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOV21531.1"
                     /translation="MEKEFYKKYWHTTSHIMAQAVKRLFPEVKLGIGPSIENGFYYDF
                     YREKPFTPKDIEEIEKEMKKIIKENYKIERIEMNKQQAKEFLKDEPFKSELLEEIQDN
                     IFSFYKQGEFIDLCRGPHLEYTGEVKFFKLLSIASSYWKGDENRESMQRIYGISFKTE
                     EELKNYLNFLKEAKERDHRVLGQKLSLFSIHPEYGSGLIFWHPKGAIIRKTIEDFWKE
                     IHLKNGYQLLYTPHIADSTLWEKSGHYSFYKDYMFPVMYMDENKKFQLKPMNCPFHII
                     IYQTKTRSYKELPLRWAELGTVYRYEKQGVLHGLLRVRGFTQDDAHIFCTDKQLENEI
                     KEILDLAFFFLKSFGFSDFSVFLSTRPEKFVGTIENWELATKTLEKSLKEKGINYEID
                     PGEGVFYGPKIDIKINDCLGRQWQCSTIQVDFNIPEKFEIKFIDEDGKEKTPILIHRA
                     ILGSLERFFGVLIEHYKGNFPLWLAPEQLRILPITEKHNQFSETIEEKLSENFRVSVD
                     LRNEKITKKIKDAEDEKIPYMLIVGDKEIQTGNFALRKHQKGMLGNFTLKEIEENFKN
                     EIEKKLF"
     gene            12613..13137
                     /gene="infC"
                     /locus_tag="PLW95_02485"
     CDS             12613..13137
                     /gene="infC"
                     /locus_tag="PLW95_02485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOV21532.1"
                     /translation="MRKDRFQDKRYRVNYKISAPKVNLIGSDGKQFGIVSRDEAIKIA
                     NEMNLDVVEIVPNANPPVCRIIDFKKFLYEQKKKEKEIKKKQKGIEVKEIRFTPGTSE
                     HDYKFKKQHIENFLKEGNRVRVIIFFKGRAILHKEKGYELLEKLTNDLRDIGKVEMSP
                     KMEGNRLSVTFIPL"
     gene            13149..13349
                     /gene="rpmI"
                     /locus_tag="PLW95_02490"
     CDS             13149..13349
                     /gene="rpmI"
                     /locus_tag="PLW95_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005364784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOV21533.1"
                     /translation="MPKLKTKKSVAKRFKITGTGKVMHYGAGGSHLLSNKSRKRKKRI
                     KKIQQLKNKGDVKTIKKLLPYK"
     gene            13359..13724
                     /gene="rplT"
                     /locus_tag="PLW95_02495"
     CDS             13359..13724
                     /gene="rplT"
                     /locus_tag="PLW95_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016340880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOV21534.1"
                     /translation="MPRTKNAPNSRKRRKKVLKRAKGYKQLRSKSYRRAKEFSEKGLT
                     YAFNDRKKKKGNFRRLWIKRISAACQMHNISYSAFINGIKKVGINLNRKSLSEIAFYQ
                     PDDFALIVEKVKKVLNEKI"
     gene            13711..14895
                     /locus_tag="PLW95_02500"
     CDS             13711..14895
                     /locus_tag="PLW95_02500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOV21535.1"
                     /translation="MKKFRNFIIFLLLVFLFYINITFTLGETFSSIFYLISFDISFSV
                     FIIIEVFYSFQLLDNIRNLQIEISKLNLSLGDYQLEGILLTTIMSLLETEELKVEEVI
                     EKIAESVKSIFKDEIVVIYLFGGRFLKKVIGEDIIELEELEEISIKNKPLLINNTSSF
                     IMYKNLSNKGITSFILTPFYTRKVVSGVLGVFSKNNRKFTLKELGLLRMVSAPTSLVI
                     ENAELFEKTKILSITDSLTSLYNRRFFETKLEEIFLKAKAEQKKFSLCICDIDYFKYY
                     NDTNGHPMGDIVLKNVAEIIKNGVKGGDIVARYGGEEFVIIFPDTEKENAIKVCEKLR
                     KKIEEYKFPYEKNQPNGDLTVSFGVATFPDDALIPDELLKKADDYLYKAKEMGRNKVI
                     SA"
     gene            complement(14885..15211)
                     /locus_tag="PLW95_02505"
     CDS             complement(14885..15211)
                     /locus_tag="PLW95_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21536.1"
                     /translation="MVMDKIEKIKEAEENAEDIIKRAEEEGIKIKENIQDMEEKFRQE
                     KEKLLKKEIENYKEAKEKEKEQELKKIEDMTNKMIEDIKKSTENKIDKVIEDALKTLK
                     DEVFMH"
     gene            complement(15371..17392)
                     /locus_tag="PLW95_02510"
     CDS             complement(15371..17392)
                     /locus_tag="PLW95_02510"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOV21537.1"
                     /translation="MVKLIYFISLISGILLGLNFNFSFFYLFLILFISIFTKRKTLIF
                     AILFFICGLLSTSRIKKDSFNDLNFDNKKLSHLKCCVVSLNKISPFSSSFIVENNGDK
                     FILELKVPANLEKGEIIEIKDVKIEKIAEKKNPWDFDSKKYYNRQGVFYTLKANYLRK
                     IGFKNIFYRSIAKIRKSGEEKIERYLPYKPEERELLKTIVIGKDKIPYFLRTLGVKSG
                     SYHLLVISGLHLSYIFLFLRIIFIPFAQLNNKHPKIFPSFALIFLWSYALITGLRIPI
                     IRATLMFTFFLLGEIFERDIEGPDSIFIAAFLMLILNPISLFNLSFLLSFITTFTIIY
                     TVRKFNFIKKPFLTFIVTTTFAQIASLPIILYNFGKFYFIGFLSNIFLIPLSVVLILI
                     SLFSFIFPFGFQISGFLANTFLNLMQTFALYTPELNCFFPIPLVITLYSFLIWALLKN
                     NKKIKDVLIFVFLLSLSFSFYQYNLPDRSKKIYFFSYKKPCFLIKEEEKGVLFSPDDF
                     KDRNFLNSLLDFIKKQKLKEITLIYTEENQLNHTGTLNQLKKYVSVKNVWESENNNSD
                     ERKEILYGYEIEKRGKNFTIKKEILDIFLALSLEEEIEGKQRIFYLNSYKKRKKIVDS
                     LNELDSVLTILPARRKKYDIIKRTFENYFLEDGCVIIELENGTLKISQL"
     gene            complement(17401..18222)
                     /gene="dapF"
                     /locus_tag="PLW95_02515"
     CDS             complement(17401..18222)
                     /gene="dapF"
                     /locus_tag="PLW95_02515"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOV21538.1"
                     /translation="MRKIKFSKIVGSGNDFVVIDNRKFLIKERNKFAISVCDRKFGVG
                     ADGLLLLENSTDADFKMRIFNPDGSEPEMCGNGLRCIIKFAYDEGIKRKKKLTIETKS
                     GILRGEIIGEEIKAELKIIGNYKLNIEVNLENKKIKGHFINTGVPHFVIITDNTDKID
                     IINDSRNIRYHKIFSPEGTNVDWITPENEKTIKIRTYERGVEDETLSCGTGSVASAII
                     SYLLGYTKMPTKIIARSGEILTISFDKNFSEIYLQGKVITTFKGHYFEKLIGEKH"
     gene            complement(18701..19675)
                     /locus_tag="PLW95_02520"
     CDS             complement(18701..19675)
                     /locus_tag="PLW95_02520"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21539.1"
                     /translation="MKKAIQDLLTPYKLGIPILEPTGKEGDFDKDMVDCPFIFWYEHK
                     FYMCYIGFDGQGYRSGLASSDELINWKRERMILDWGEKGRFDSYGAAALSILLENNQL
                     GELPIPMKWQGKYWATYTGYPEKGYEKGPGSVGIVYSDNLINWEKADFNPVMVPSDGM
                     KWEQGGIFRTFLMKEKGIFYLFYNAKPAFSGDWIEEIGFATSKDLKTWVRYSNNPVIN
                     RNNANNWDLVVGDPYLVKYKDKWVMFYCGVDKKNARDGIAFSVDLKQWEKVPEPILDI
                     GTDEDIDGKHAHKPCVILYKGILYHFYCAVDKNDYRTITVATSQHLGK"
     gene            19929..20366
                     /gene="dtd"
                     /locus_tag="PLW95_02525"
     CDS             19929..20366
                     /gene="dtd"
                     /locus_tag="PLW95_02525"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017637067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOV21540.1"
                     /translation="MRVVIQRVKKASLYIENSYKGSIDKGVVVLVGFKKGDSESEVKY
                     ISDKIFNLRIFEDENGKMNYPIGKINGDIMLVPQFTLYGDVKNSNRPDFSNAENFSKA
                     NTLFDIFKNEIRKTGLNLLCGEFGKEMLVEIHNDGPVTIIIDK"
     gene            20369..20632
                     /locus_tag="PLW95_02530"
     CDS             20369..20632
                     /locus_tag="PLW95_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21541.1"
                     /translation="MRNNYKNEKVVVDTSTNWVYIGILKDEDNTYYILDDADAFDISE
                     TSLSKNEYLMMVKKDGIVPNRKKVKILKLKVVAITLLSDILEK"
     gene            20632..20805
                     /locus_tag="PLW95_02535"
     CDS             20632..20805
                     /locus_tag="PLW95_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21542.1"
                     /translation="MEKLLAIFFGFISGMIVYSGVGFYFTRKIELAKKYKFRLIVFSI
                     ILVVSLILKAVIK"
     gene            20802..21161
                     /locus_tag="PLW95_02540"
     CDS             20802..21161
                     /locus_tag="PLW95_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21543.1"
                     /translation="MNRKERMIFRKNLYKKILKKKNDYYEVLVQLSNIYSILNFYKKA
                     LEIDKKIVELSPSDPIACYNLACDYSIIGDIKKSVKWLKNAINYGYDDFEYMENDPDL
                     ENLRKSGIFKCIFNKNN"
     gene            21163..21750
                     /locus_tag="PLW95_02545"
     CDS             21163..21750
                     /locus_tag="PLW95_02545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOV21544.1"
                     /translation="MRNFFYICAKLLGFSLFKILFDWEIKGKENIPRKGGFIIASNHL
                     SYLDPPIIGIASPRKVHYLAKSSLFQIPVLNFLIKIYGAIPIEREKEYSISIRKGIEI
                     LKKGEGLVIFPEGTRNPEGNIEMPKKGVSFLAYKTGVPVIPTKLKGTEKALPKGKKFL
                     KPAKVKVIFGSSIKVENNDYINQANRIMKIIKELN"
     gene            21747..22598
                     /gene="ispH"
                     /locus_tag="PLW95_02550"
     CDS             21747..22598
                     /gene="ispH"
                     /locus_tag="PLW95_02550"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOV21545.1"
                     /translation="MKKIIIAKDIGFCFGVKRAVKIAEEILEKKGKLASLGDIVHNPF
                     VMEQLIKKGLKVYREEKEIKNVPFIIRSHGLPVSKIKTLEKRQIEIYDATCPYVKKIH
                     YLIENLDKENFFIIIIGNKFHPEVISLKDYGRNIFVLDEEIKNCNKKFEKVAIIGQTT
                     LSFKKYLELGGKLIKKIESKKTIVYNTICKVTENRQKQATEISREVDAVFILGGKSSS
                     NTTKLYELCKLNNKRSFHIENLEEIDKIDLKKFNSIGIVSGTSTPEEFIDRVLNYLKK
                     NDFKEVI"
     gene            22599..23945
                     /locus_tag="PLW95_02555"
     CDS             22599..23945
                     /locus_tag="PLW95_02555"
                     /inference="COORDINATES: protein motif:HMM:NF004952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOV21546.1"
                     /translation="MKNEVSKLLEEKISSFKPGSLVKGTVVRISNDEVIIDIGYKSEG
                     AAPREEFKGMQDDVKEGMEVMIVVESLDPDSNGLIPLSKEKADITLNWENIEKHFNED
                     KPIEGVIFKRVKGGYRVDIGVTAFLPKSQTDIKPIADPNECLETKSYFKIIKYDPLRK
                     NIVVSKKKYLEEEREKEKFDFLKSLEKNQLVTGVIRNIVDYGAFIEIDNGVVGLLHIN
                     DMSWGRISHPSQLFGIGEKIEVVVTNVDLDKQIVSFGLKQKTPNPWENIEEKYPVGSV
                     VEGKVVNITEYGAFIKIDEGIEGLLHISELSWTGRIKHPSEIVAMGDTVKVKVVDIKK
                     DQQKISFSLKQLEPNPWPEIAKKYKVGTVVKGKVYNITDYGAFIEIEEGIDGLLHISN
                     ISDQPIKHPSEVLRKGQKIETMVLEINSDEKKINLGLKQLGEIVKKGEENESDSEE"
     gene            23923..24717
                     /gene="nagB"
                     /locus_tag="PLW95_02560"
     CDS             23923..24717
                     /gene="nagB"
                     /locus_tag="PLW95_02560"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011461409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOV21547.1"
                     /translation="MRVIVKSNYEEMSEEAAKIVAELVREKGDCVLGLATGSTPVGLY
                     HQLVRMHKEEGLDFSKVKTFNLDEYYGISPQHPQSYRYFMNENLFNGINIDKKNTFVP
                     DGTIPLEEIEKYCDEYEKMIKKAGGIDLQVLGIGSDGHIAFNEPGSPLSSRTRLVALD
                     KQTIEDNSRFFDNIEDVPKFALSMGVGTILEAKEILFLVNGEKKAEVVAKAIEGPITS
                     LITASSLQCHQKVTTILDEAAASKLKRIEYYKFKTESEKKINKKLF"
     gene            24720..25688
                     /locus_tag="PLW95_02565"
     CDS             24720..25688
                     /locus_tag="PLW95_02565"
                     /inference="COORDINATES: protein motif:HMM:NF007233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOV21548.1"
                     /translation="MGLIENFIEKAKRNKKRVVFPEGEEERLIESAIKLEEDGIARPI
                     LVGRRNIIKEKIEALKIPSDKIEIVDIENNPYIDEFANVYSEKRKIKLSIAQRLIKRE
                     LIFGGMMLLTDKVDLMIAGAANTTASVIQASSLTVGYKQDTSTPSSFFIMVLPDNKIY
                     FYADCAVNIDPTYEQLADIGISTGENFECLIGEKAKVAFLSFSTKGSASHPFVEKVQK
                     AVEIAKSKNRNFPVDGEFQADTAIVSVVAEKKLKEPSEVAGKANVLIFPDLNSGNISY
                     KLTQYLAKAQALGPILQGFAKPVSDLSRGAKVEDIIKTTAILSCMC"
     gene            25836..27086
                     /locus_tag="PLW95_02570"
     CDS             25836..27086
                     /locus_tag="PLW95_02570"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HOV21549.1"
                     /translation="MKILIFEDENWKNLLPLSYIKNTFELRCGYYTLFERIAQNFNGE
                     KGLWVRDYISSLTKIKFNLPTNDENFIKDDIFIINGTTIIDGKVNIEEEKIFVDEYNN
                     ILYGYIKKETVEKFWEGNFWKFLSNLKENIKKEKVELISIKYPWNLIHFNGQLLKTDF
                     ENIQNKKIEGEVSGKCEIINEKNVVIRNEAKIHPFVVLDASKGPIFIDREAEIFPFAR
                     IEGPSFIGEKTQIMPGANIREGNSFGPVCRIGGEVEESIFHSYSNKYHDGFIGHSYIG
                     EFVNLGALTTNSDLKNDYSDVVVYLCGSPTNTGDLKVGSFIGDHTKTSIGTLFNTGTI
                     IGIMCNITAGGILPKYFPSFTWYINNKFMKGTGIETGIETTKKAMARRGRDLPKEEEE
                     VIRYLYQETETERKEYVKRSRKKQ"
     gene            27099..27347
                     /gene="acpP"
                     /locus_tag="PLW95_02575"
     CDS             27099..27347
                     /gene="acpP"
                     /locus_tag="PLW95_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOV21550.1"
                     /translation="MEEKEIEERAKKVVSSILGVDEEKIKSESRFVEDLGAESVASLE
                     LVAGFEEEFDIEMDENAALEVKTFGDAVKFIKKYIEGK"
     gene            27344..28546
                     /locus_tag="PLW95_02580"
     CDS             27344..28546
                     /locus_tag="PLW95_02580"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOV21551.1"
                     /translation="MKVLVFNCGSSSVKFKLFEMPEEKQIVEGSVEKIGEEISFFTLK
                     KESEANKEELKIRNHLEALQLIAKSLVDKKNEIISNLDEIEGVGHRVVHGGEGFDKSV
                     VIDEAVIKKIEEYQGLAPLHNPHNLAGIKAGMKFFPKSVQVSSFDTAFHSTIPEVAYI
                     YGIPYKFYEKYQLRRYGFHGTSHRYVARRTSEIMGKGKYDINVITCHLGNGCSVTACK
                     NGKSVDTSMGLTPLEGLIMGTRSGDIDPGIIFYLGESNGLSFSEINKIFNKESGLLGI
                     SGVSNDFRNLMENIENPRVKLAIEIFCYRLKKYIGSYIAVLGKIDAISFTAGIGENVP
                     YVREKSLENLENLGIEIDKEKNKKITERKEGEISKESSKIKVFVIPTNEELRIAYDTY
                     QLTQKTKL"
     gene            28543..29535
                     /gene="rfaD"
                     /locus_tag="PLW95_02585"
     CDS             28543..29535
                     /gene="rfaD"
                     /locus_tag="PLW95_02585"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013910237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HOV21552.1"
                     /translation="MNKKYIVITGGAGFIGSNLVQYLNQKGIENIIIVDHLTNEKWKN
                     LIGKKFIDYIEKNKFLKLISEGPYGENNKLPFEIEKCVHLGACTSTIEKNVNYVIRNN
                     FLYTKKLAEYCFSKGIYFIYASSAATYGNGENGFSDDEENIFSLKPLNIYGFSKHLFD
                     LIMVKNDLWRRNFCGLKFFNVYGPNEYHKGEMKSVINKMYYQIIENGYVTLFKSYRDD
                     YKNGEQKRDFIYVKDVVDVIYFFLQNKKCGIYNVGTGKGESFNKVAEVLFTLLKKEKK
                     IKYIDMPVEIRKNYQYFTEGNISKLRSAGYSKEFTSIEEGISDYLKYLENGIYE"
     gene            29692..29988
                     /locus_tag="PLW95_02590"
     CDS             29692..29988
                     /locus_tag="PLW95_02590"
                     /inference="COORDINATES: protein motif:HMM:NF014092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HOV21553.1"
                     /translation="MGKIIFVGEATSIEFFKNFGFEIIPATSKEEAKEILDKKNYQDI
                     EVLFITEEIFDSVVFSRFVDEKKIVVIPSLKSNKGKGYKIVERLISKATGMKGE"
     gene            29991..31733
                     /locus_tag="PLW95_02595"
     CDS             29991..31733
                     /locus_tag="PLW95_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOV21554.1"
                     /translation="MVKGEIIKVSGPLVVAKGMEKSKMFDVVRVGDEKLVGEIIKIEN
                     GIASIQVYEETEGLKVGDPVISTELPLTVELAPGLLSTIFDGIQRPLSEIEKRGDFIR
                     RGIDVPSIDRNKKWDFKPVVKKGDIVEGGDVIGAVQETPLIVHKIMVPPDLKGEIVEI
                     KEGSFTVEEEIAKLKTENGYVSLKMLQKWTVRGKRPFKKRLPPDTPLVTGQRVIDTLF
                     PILKGGTACIPGPFGSGKTVVLHQIAKWADSQIVIYIGCGERGNEMADVLMEFPELKD
                     PASGRPLLERTVLIANTSNMPVAAREASIYTGVTIGEYFRDMGYSVALMADSTSRWAE
                     ALREISGRMEEMPGEEGYPAYLGTRVAEFYERGGKIICLGKEEIEGSLSIIGAVSPPG
                     GDLTDPVVQMTLRVVKVFWGLDDRLAYQRHFPAINWLTSYSLYNENVKEYLNREISED
                     FQKIRNEGVSILEREDELLEIVRLVGMETLSEEDRLILETARSIREDFLHQSAFDERD
                     AYTTLKKQYLMLKTIILFHRFAIEQIKKGKSLSDILKHPVRADITRMRYISENKLDDF
                     NNIINKISSIEEKK"
     gene            31774..32628
                     /locus_tag="PLW95_02600"
     CDS             31774..32628
                     /locus_tag="PLW95_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HOV21555.1"
                     /translation="MANRFKTYFLLILLTVLFVFIGSFFGKSGMIIAFLIALALNWFS
                     YFFSDKIVLAMYRARPVSYNEAPDLHGLVEQLCQSGNIPKPSIYIIPSQSPNAFATGR
                     DPNHAAVAVTEGILEILNEGELKGVLGHELTHVKNRDILIATIVATIAGAITMLARML
                     QWAAMFGGVDRDNRKGGVFSLIAMLLFAILAPIAAMIIQLAVSRSREYLADEGGAKLC
                     GNPLYLANALKKLSEGVRRNPMTTSNPSTSHLFIVNPFSAKGLVNLFSTHPPMEERIK
                     RLENMLVR"
     gene            32625..33365
                     /gene="larB"
                     /locus_tag="PLW95_02605"
     CDS             32625..33365
                     /gene="larB"
                     /locus_tag="PLW95_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HOV21556.1"
                     /translation="MNNEKLKKVLTDYKNGKISEDDVFQYFLYFPYKDLKFAKIDTYR
                     NLKFGFPEVVYGKEKKLSHLVKIVENMMKLHSNFIITKVDEKIAKKLRNKFPSLTYFP
                     DAKIMSVKEAKIDKKKGYICVVCAGTSDIPIAEEAAITAEILGNKVERIYDVGVAGIH
                     RLLDKIEILNKAKCIIVVAGMEGALPGVVGGLVGKAVIAVPTSVGYGTNFSGLTPLFT
                     MLNSCSPNVVVVNIDNGFGAGFFANLLS"
     gene            33373..34620
                     /locus_tag="PLW95_02610"
     CDS             33373..34620
                     /locus_tag="PLW95_02610"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOV21557.1"
                     /translation="MGISEKDKTILRDLAKKVKEISLNPIWQEKINLWKTHNKLGKTR
                     PMVMIYLGDGDVWKDIIPEGTLLCEDDYLRKIEFNLRERIYRWENLKDDTVIDGRIFT
                     PIFVENTGFGITEETLKPEEYGGAVHYHAVIKEEKDIEKIKKPKISVDYKRTEKTFEL
                     VSNILGDILKIEKRGPCGFWFAPIDMFAKWRSFDQLFIDMIERPQWLHKVLNFITECT
                     LEEIEFYEKENILGFNHDLNYEGSGGAGYTDELPQNDFDGKHVRTKDLWGHAATQIFS
                     LVSPSMHEEFAIMYEKRLLSKFGLSSYGCCEPLHKKVDILFKNIPNIRRISMSPWVDI
                     KEGAKNIEDKAIFSWKPSPSILAGEIFEPENVRRIIRDGLEKTNDCVVEIIMKDVRTC
                     REKPERLTEWVNITMEEAIKYLK"
     gene            complement(34633..35676)
                     /locus_tag="PLW95_02615"
     CDS             complement(34633..35676)
                     /locus_tag="PLW95_02615"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOV21558.1"
                     /translation="MSVEIARENINLKRKGKIAHTEYSLVYHKEFLKKITELSENDPD
                     FEKIAYNKLCIDFLFHTNDGLINWGKTGRITDMGHAEYSSDGSDKREKEKSPFRTEED
                     VWEFDAVEEYGLTDFDKQVKEYEKIIEDMKIKFPEQLIPGGYYKTIVSGAIQAFGWEM
                     FLLAASNIGKLERVLDSFFRRTMFFTKAWAETSIEVFIQHDDFVWTSGPFMKLEIYKK
                     IIIPRYVSLWKVLHEKGKKVLFCSDGNFMQLAEDIVEAGADGLIFEPCNDFGYMFENF
                     GKKCCLVGSYVDCRDLVFGRIEKVKKDIDRTIEKFFETKGGIFAVGNHLSPNIKPEIL
                     EQFFEYLIERLQR"
     gene            complement(35673..36995)
                     /locus_tag="PLW95_02620"
     CDS             complement(35673..36995)
                     /locus_tag="PLW95_02620"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV21559.1"
                     /translation="MEKKDRLKETVSTIVKPVTFFIDKFNAIIKRDDEEGIKEVDNFV
                     YSLLDQVKDMEEGMRAFFVEILFINQLTEKIAEVVNEITLSEILGERIRKFLKTDIIS
                     IYLLGEKELSPVYSYPYDKEKIDKLHQLASDSFIKGESLIFPKVNLNSKKYNILSVPL
                     RTTKEKFGQVLIGKRKSFTKEETLILISGCSIISFIMSNMRLTQEMIKNERLATIGKV
                     ISGLSHDIRNHLQKIENGLYLIETGLKGKHRKEVIIDGVNILKGSYEKMKNLVLSMVD
                     YTKERELQLQLTDINNLLDTIIKENKDLFKENKIKIKKEFGQNIPQIYIDPYKIERGI
                     TNLLLNAVDAVEGKQGIIKVGTRYNSEKEIIEIWVEDNGCGIPEQNLDKIFDIFFSTK
                     GSGGTGFGLAIVQKVVKEHNGIVEVKSKIGKGTKFTLKIPAKKNVEGK"
     gene            complement(36997..37725)
                     /locus_tag="PLW95_02625"
     CDS             complement(36997..37725)
                     /locus_tag="PLW95_02625"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HOV21560.1"
                     /translation="MERYQLAEKIIEKSGKFLEENFGKGKEKENFRFDVKIFQDVESE
                     KIILEEIEKNFPDDGWVSEEKGEKKNKTGYIWIIDPLDGTFNYLRKIPHFAISIACKG
                     EKEEFGIVYDVMKKEKFTGIKGKGAFLNGKSIKVSEIKTLKESVGIIGFMKGNDEIKI
                     GIEIIKKYAPKIKKIRMTGSAALDLCYVACGRVDFYIEFNLKIWDIAGGKIILNQAGG
                     NYIEKISDGKKISIATNNFLKIGI"
BASE COUNT        13532 a         5331 c         5723 g        13257 t
ORIGIN      
        1 aacttttact aaaagtgatg tttctgggtc aatccaggaa attttgtaaa atataaaact
       61 attcttctct tctttttcta tatgtttttc ccatttattt ataaatgaac ttgatggaat
      121 attattataa ataaatgaaa aaggaggatt ttcactttct aataataaaa agttgttttg
      181 acattcatct aaccatataa cttctccatc ttccatgatt atctgtgcat ctgcccaatc
      241 cgctcttgga tgggataaat caccagaaac agaattaacc tttaggatta tttgttgaac
      301 agaagaaagg ggaatttcta taaaaaatgg tttatctcct ttttttaaaa caggtgtttt
      361 taccaattct tcgtctttcc cttcaatact acctacaaca cttgctataa actgctgact
      421 gttataatta ttatcatagg tatcatcaat cccaactcca ataaatgatt tgaaaacttt
      481 tgcacctttt gggatattaa caaggatttc actattagca tgcgtactta aaccatgctc
      541 aaaacattta tcaccaattt ttataggtaa ttcaccaaat tttattacag gaaagtcctg
      601 cttgataaat tttaactgaa ctggtgcagg aaaaagtcgc ttatctgtaa aacaaataga
      661 tgcccaattc cccacttgct tttgtgtttc ttctatttct cttttatcca tttcctttcc
      721 tctcttaata aatgtgtcaa gattttttat atttatattt gcttttagtt tttcaatatc
      781 tttacactct ttaatttctt ctcttttgct cttagcctta ccctctcatt tcttcctgtc
      841 ttaccgattt atctgtgtcc tattcctaca ttacctcatt cagtgtctaa gagcatttta
      901 ctggcagagt ggtctattct taattcccag tgtctcagat atacgcagcc ttatggatgt
      961 tgaccgatct caccctgtca cttttatctt tatgaaaaaa cttcttaatt tcttctagat
     1021 tgtataaagt tttttcctta catacaataa acattatcct taacatcttt gctgctatcc
     1081 cagtaagtac tttcatcttc tcttttttat tctcctttac tttatatggt aaggtatagc
     1141 ccaaggatag ggggaaagaa tgagggaaga attaaagcaa gaatataaat ttgttttcaa
     1201 atatagagaa acacagttgt tcatatgaac aactgaggtt atggggcttt taaagaatcc
     1261 cagaccaaag ttcaggagtt ctttacttca aattctctat taaatatcct attttcaaac
     1321 gatatgtttt taaaatttct ggtgaagtaa tattattttc tacaaaatta tcttgaaata
     1381 attcatttag aagtaaattt gctgcatcta aatattcatt attatttttt tcatccaatt
     1441 ttattttttt aaccttatct tcaagaatag aaatgtaatc aaaatcgttt atcccagcag
     1501 ctaccatttc ccaccttatt gtattaattg gctttgtttt tgttggataa agaaaatgag
     1561 catttccatt tgagccagca acaagagggt ctttccatgg attatcattt ccataaccgc
     1621 caatacaatt ccagtataga caaccacaat aaccataact tttaatgcgc caaaattctt
     1681 ttcttaaatt aaaatttgat gcctctatat ttgcagaacc tatattagca gaccaaaact
     1741 catctcctgt ctgcatcctc tttcttatcc attcagacct ttgaagtcct ctacaccaga
     1801 tattaacatg tccaaaaaga tattcaggat tatctccata tattgttccc aaaaatttaa
     1861 gtttgggctc tattttatgg agatattcac aaaactcctt aatcttagga tataaattag
     1921 caggtgcctc atcataagta taaacaaaag caatatcagt tagtcctgtt ttttgtaaaa
     1981 ttctaacaat ttccttaaat tttatctcat tcttcactaa aaaagatggt atatacaaag
     2041 atgtaatttc tttttcttta taataattca aagcatttat tatttcattt tcatctccat
     2101 cccatggaat atttttttct cttggtgtaa ttctgtgttt aagataattt tcctgacata
     2161 attgctgaaa attttttgag ctcccttgaa atcctatttc tttcaataaa ttctctgata
     2221 aaaaccaaca accaaaatca gtctttaaat atggtttttc tggtaaaaca aaatcaaaaa
     2281 ttttaagttt tacaggtgtt tttattactt gattcttccc aatttttaca cttaaagagg
     2341 atttataaat tcctgaatta atattttttg gaatatttac aataacccaa actggtatat
     2401 ttgtttttgg ttcaactgaa aaagaagatt cagaaattaa tgggtctgga tacaaaccaa
     2461 taggaatatt tgcacttaat cccgatggag tatatgttct tacataccct actttatata
     2521 tttctatttc ttttaagcca attttgtctt ttcctgatat aaaaggattt atatcaattt
     2581 ttaccaattt attttctgaa gaattatttc taattactat ttgaaaagct actctttctc
     2641 ttttagcacc atatatctcc aattgttctt tttccccaat aggagttagt atatcatctt
     2701 tgaatacctt ttcctgagta tctacttccc atatagatat tccggatgtc tttttaatta
     2761 aataaccccc ttgataccct ttttctcttg taataaatcc tttccttgtt tttttccatt
     2821 cagataatga aacatcctca aatgaaatac cttttttcaa aggaaatata attttatttt
     2881 cttcactttc aatagtttca cctaacttta tctctttgtt attttgatta aacactattt
     2941 ccaatttacc ttctgaaaaa ttttctggaa tttggagaga aatttctaat ggattatttc
     3001 ctgccaaaat tatttctttt gtttttttaa gaatctctcc tttttccaaa ttatctttgt
     3061 taaataatgt gaaaatgatt tctacttctc ctaaatttat tggagatgct aattcaacta
     3121 ttaaattgtt ttcctgccat tgatacccag aaactatttc tggtgaaacc cacccaactt
     3181 ttgaaacttg aggaataatt ccaacataat acaatgtttc ccatttttca cctggtgcta
     3241 aatttatcgt attataagac cattctatac tatgataact attccaatag cagtaatagt
     3301 attttgtaat atcacaatat ttcggggtaa aataaagaaa aaagttatca ctataagcac
     3361 ctatccagtt tcttacaaca tcaacaaatt tatcagcaga tggttttata tacttaattc
     3421 catctaattg tggtaaacaa gtaaaagata attttctatc tacaatatta tgaacccaag
     3481 gtgttaatga aattttttcc ttcctatgat ttataatttc ccatactatt gcaactcttg
     3541 tctcattatc tttcaatata tactttttta taaattctat atctttattt ttatcacttt
     3601 ttgataaata agaaagagta aaaatttgtt tatcgccttc cctttttaac tccccttcat
     3661 attttgcttg attaatctca ccttctggat aaccctgccc agcaagtcgg tcttcagcaa
     3721 taccaccagc ccaatattta cctgtaaaaa attcctctgt taattctctt tgtgctggtt
     3781 taaaaacaat actacgaatc actccacctc ttgctttatc aatagtcaat ttcaaaaaat
     3841 tattttccat aataactggc tccaccaaat tcctttcaaa acttacctga caacataatg
     3901 acttataaat taaatgagac aaacaaaata tcaaaaatac taataaaatt tttttattca
     3961 ttatttctcc ttttctttta aaatttcatt tatgattttt tcccttatta taaaaaaatc
     4021 ctcatttgaa atagttaaaa tagaagtaaa tgaatgtaat tattaaaaaa agtttattct
     4081 ttcccaaaaa tttctacctc cgttaaattt aaccatttat tttctaatgc ccatgtcgtt
     4141 attctaatat atctttcatt tatattaagg tcagtaaatt caatattcca attaactgtt
     4201 tttggtggtt caactttttc tataactttt tttatctcat atacaatgcc atctttacta
     4261 gaatataaaa cagatttact tacaccaaac atgggagaaa gattttgacc tttaagtact
     4321 acatttgtta tttttcttgg cttttttaaa tcaaaatcta ttgttataaa tgaatcatca
     4381 ccaggaactc tccaaaacac ataacaaagc cctgtattag gggcataaga atccctccca
     4441 tcagttagta atcccttcct ttcttcaaaa tctccatctt cattgactat ataataaatt
     4501 ttatctattt ccccatcctt tttaatactt gcttttccaa taacattatt tttttcatct
     4561 aatatgttta attcctgctt tcctaaattt gaaattttat cagaactttt ttcaattgaa
     4621 aacttaatat atcctttttt ttctttaaaa tctatttcaa taactccatc tttattattt
     4681 ataatttctc ctataacctt cccttcttta tcttttataa tatttccttc aacaattccc
     4741 accctatatc ttattaaatc attatctctc tttttcccat attgcggaac atcaaaatag
     4801 tgtccaacag atactttata agaaacacct tcttctttcg caaaatttat cattttttct
     4861 tctgaataaa ttaaatgaga caaacaaaat atcaaaaata ctaataaaat ttttttattc
     4921 attacttctc cttttctttt aaaatttcat ttatgatttt ttcccttatt atagaaaaat
     4981 attcatttga aatagttaaa atagaagtaa atggaggtaa taaactaatt actttttctc
     5041 tcgccttttt ctccccaatt tttttactta aaatatcttc aagtgttttt aaatatttat
     5101 aatcagtaat tccttctctt aacatttccc atccaattgt agagtacatt ttgccttcat
     5161 catttaaaaa cggccaggca atataccagt tagaagtccc ccaattataa gcaaatcccc
     5221 aaattccttt acaacctgta taataaagat atattccata cttttttctt atttcataag
     5281 gcattgtgct tcttacttgt gtttgaatat gcattttatt tatattggct tttacccact
     5341 ctggttctac tgaaccccat tgaggaaaaa agaaatttgt atagttttct tcttttaatg
     5401 catcttctat accgcctact acacaattta tatctatcag ttgataccac ttacgaaatc
     5461 cagcaacagt attgtttgac caaattttgc cattggcttt ttttatacat ttaagtagtt
     5521 tttcataatt ttcaatacaa tcagggtgaa catctggttc atcagttgga tgaaaatata
     5581 atttcattcc taactcatct gctaaactca ttatttcttt taaaacaata tcaacaacat
     5641 tttgatattc aggagtttct atctcttgcc cagaaattgt ttttatctgg ttaaatactg
     5701 gttctaatgt tccataagga aatggtccag tagtattaag tccttctgtt ttatatactt
     5761 gtaatgtctt tttcaatctt gaagtatcta ctaaaatttc atcttgattt ttcttaattg
     5821 taattggaga tgtccatggg tctgttgtaa tactgttaat accatgattt tttaagtcct
     5881 ctattctttt tgatgttaaa ttatcaaatg cccctggtaa tttattatcc cagagcccaa
     5941 aaatagtatc tacttgagaa agttcaaatg gtaaaatttt aacttttatt ggaatatata
     6001 tttctttatc accggatttc aatattactt tccctgtcca atctgtagat gaaatatctt
     6061 gaggaatttt tatccttaac cataaggaag atgaactttt tgctttgacc tcccatttat
     6121 tagatggatt tagaatatct ggatgaagaa tttcaccagt tggatttata aattcatata
     6181 tagtactttt tgttggtgaa aaagtcagga caaaaacttc tatgttttct tttgggataa
     6241 atttattgtc agggctttct aatggtgttg taataatatc aaaaattata ttttcttcac
     6301 tttcatttct taaagaaagg gttataggaa taatttctcc aggagtgcat actttttcaa
     6361 tttttacatt acattcttct tctcttggaa aactacaagg aaaaataatt ctatctgaaa
     6421 catgtgtaaa aaataaaagc ggttcagaaa atttttccag tgtaaatgac atttcaatag
     6481 agtaaatttc attgatattt ctaaaactta tctcatttat acccttaata atataatctg
     6541 atatattgaa agcatagaca taatatgcat tttcaaattc atatttattc catgaagtat
     6601 ttgcattact aaaacttgaa atacgattat atgggtctcc atttacttct gaattataag
     6661 gggagtagtc actatcaaat cttaaaaacc atgcatcttt gtcttcagaa aataaatttg
     6721 ttggtctttg tccaatataa gcagaattta ttagaattaa ttttttgttt attaacttct
     6781 tttctgaaat tgatttttta tttattgaaa cttgaagtcc atagtccccc catccttctc
     6841 ccctttctat attaaatcta ccctttatcc ataaaacagt ataaccatca ggattagaaa
     6901 cattaaaatt aaaacttttc tgttctttcc cttttagaat taatttttgt ttttttatta
     6961 aattctcttg actaatattt gcctcagaaa aaagtaaaat aggtgtaaat aaaacaagat
     7021 aaatcacaaa tttaaaactt tgtcgcattt tttttctttc ctctacaaaa agtaaattgt
     7081 ttatttctgt aattcaccat ctccagcgaa aacctgccca tgttgtcccg attcctgttc
     7141 ttggccaata actatctgga atccattcaa catggccatc aagaaacagt aaattcattc
     7201 ccccagagtg tcttgtataa accgcatatt ttccaagatc agtgtatcca taatatccat
     7261 caccatataa actttttcca tcaaatacaa ttggagtttt agtaggagtt ggatttcgag
     7321 aaactttctt attagctgct ccactattat aagcatatcc aactttataa aatggatacc
     7381 agtcataata tcctgtttgt aaagcagatg ggcacctaaa tgttgtgaat ggaattgccg
     7441 gttttaaggg ttcccatgtt gtaggtgttc ccggtttata agtaattcta cttattaaac
     7501 tgaaccagca attagaagtc atcccaactc tgttaggagg ggtatatcca tcataatcat
     7561 cacaatacat taaaaaggca tatcctatct gttttaaatt actcatacat actgactgtc
     7621 ttgccttttc ccttgcctga ctgagtgccg gtagtaacat cgctgccaaa atcgctatta
     7681 tcgcaattac cacaagcaat tctattagag taaaccctcc gccacaagac gcggtggacg
     7741 ctgaaatacg cgctctccct ctattcattt ctatcacctc ctatcttaat aaatctgtca
     7801 agatttattt atatttatat tttcttttaa tttttcaaat atctttatac tctttaattt
     7861 cttctctttt gctcttagcc ttaccctctc atttcttcct gtcttaccga tttatctgtg
     7921 tcctattcct acattacctc attcagtgtc taagagcatt ttactggcag agttgtctat
     7981 tcttaattcc cagtgtcttt tcttctctcc aaactctttc cctttttaag tcctgcccgc
     8041 catctttgta gacggaaaga tggagaggaa tttcttcttc actttattta tataacaact
     8101 cttttatttt gtcaagtcaa ctttgccatt tcattttggg caattatgaa aacacttgta
     8161 aataaatggg ttaaagagat tttaatttgt tcactacatt tcactacata tttgcagaaa
     8221 cccgtatgaa ataaggtttt ttcatttttg agtagcgaac ttgacttgtc aataccccga
     8281 attgcctcat aaaaaaatcc atataaaaaa tgaggacttc taactcaaag aaacaaataa
     8341 gcaaaataat ttctttcttt gagaaaccaa ctgatgtagt aaaaaattag taaggaaaag
     8401 ttgtcctcgt agtgtatcca acagatacta caagaataca aaaacgcctt tctcttttat
     8461 ccatttctca tgcatttttt gacattattc agaattatta catttactta caattacttt
     8521 ttactaaatc acttatatat tcaacaacat tttcaacatc aacttcttga tatgtttttt
     8581 cttttctttc tcttacttca actttccctt cttttaattt ttttcctaaa acaatcatta
     8641 taggtatccc aataagttct gcatctgtca attttactcc tgctgtttca tctctatcat
     8701 caaataaaac attcttttta ttaggaaaat cctgataaat cttttctgat gtttctttga
     8761 catctgtttg ggcaataagt gggattataa ttactttaaa tggagcaatt gatataggcc
     8821 agataatacc tttttcatca taacttccct ctattgccgc agcaattatt ctacttactc
     8881 ctattccata acatcccatt acaattggtt tttcttttcc atctctatct acaaaatttg
     8941 ctttcattga cctgctataa tcagtcccta atttaaaaat atgtcctact tcaagttcat
     9001 ttaagtcccc ttctgccaca aattcttctg aaaattttcc tcctataact cctgaatcag
     9061 cagttcttat atttgtttta agaccacatc ttgaaaaaat ccttatatat gcttctttca
     9121 ttttttcata tgaaatgtcc aagccaacct catcaatatc aaaactataa gcatctttca
     9181 ttaaaaattc tcttgctctt ataattccaa atcttggtct tatttcatct ctgaattttg
     9241 tctgaatttg ataaagcaac aggggaagtt gtttccaact ttttatatgt cttctaacaa
     9301 ggtcagttat aatttcttca tgagttggac caaaaatcat ttctctttcg cttctgtcaa
     9361 taaatttaat catatctttt cccattttcc cgtatcttcc actttctttc cataaagaag
     9421 atggttgaag tgaaggcatt aaaatttcaa ttcctccact tttattcatt tcttgcctaa
     9481 tgatattttc aacttttctt aaaaccctgt acccaagtgg aagatatgta taaacgccag
     9541 aagaaacttt atcaataaat ccacctctca acataagttt atgacttata ctttctgctt
     9601 cttttgggac ttcttttaaa gttggtataa aataattact ccatttcatt ttttactcct
     9661 tttttattat ggtactgcca aaattaccta atatctttta taattctttt ctttatcttt
     9721 taaggtgttt ataagtattt atatccctcc ccctcatcta tcttctccct ccgccacaat
     9781 actcggcgga caggcacagg ggagaggaaa aaaggaagag agcatttccc ctctttttta
     9841 tctaattttt taacactacc tttatgtctt ttaattaaca aatcatgttg aatattatta
     9901 taacttcaaa acaggaattt aaaaaacttt tattattgct taattttttt aaataagatg
     9961 tgttattgac aaaagacatc tttttattgt attataattc ctgaacaaag gggccgtagt
    10021 tcagcggatt agaatgccag actgtcgatc tggaggtcgc gggttcaaac cccgtcggcc
    10081 ccgctaatta tgtaaaagca aggaaatttg gaaagtagag gagaggaaaa taactcccgc
    10141 gaccaaaagg gtatgggaaa agacattttc ccatatttat aataatctaa taaagcaatt
    10201 cgtagtgggg gtatcgttgg gaaaggggaa gcgcccctgt cccaggagcg aaagcgacgg
    10261 cgcgggttca aaccccgtcg gccccgctaa gtattctaac tttgtaaggg agttgaacct
    10321 tttgggttca aaccgactaa agttgcttac cttgttcctc agagttggtg tttgatgttt
    10381 tttccaagca caacgggagg ttgtggagat ttttaaattt ttcccctcac ccgaacctct
    10441 cccctcaagg gagaggagaa aaaagaggat gatatatttc cttctcccca tcggggagaa
    10501 gattaagatg aggggggatt ttaaatgtta agataagggc atttaattgg tttataaagg
    10561 aaggaaataa taaaaatttt catcttctgt aacaatagcg aaaaaaattt ttttaaggtt
    10621 taaatacttt aatgcaactt aaatatgaaa tgccctttaa aatttaaaca aatggaaaaa
    10681 gaattttata aaaaatactg gcatacaaca tctcatataa tggctcaagc agttaaaaga
    10741 ttattcccgg aagttaaatt agggatagga ccttcaattg aaaatggttt ttattatgat
    10801 ttttatagag aaaaaccatt tacaccaaag gatatagaag aaattgaaaa agagatgaag
    10861 aaaattataa aggaaaatta taaaattgaa agaattgaaa tgaataagca acaggcaaag
    10921 gaatttctaa aagatgagcc atttaaatct gaacttttag aagaaataca ggacaatatt
    10981 ttctcttttt ataagcaggg tgaatttatt gatttatgtc gtggacctca tcttgaatac
    11041 acaggagaag tgaaattttt taaacttctt tccattgctt cttcatattg gaaaggagat
    11101 gaaaataggg aaagtatgca aaggatttat ggtatttctt tcaaaacaga agaagaacta
    11161 aaaaattatc tcaatttttt aaaagaagca aaagaaagag accacagagt tttagggcag
    11221 aaactatccc ttttcagtat acatcctgaa tatgggtcag gtcttatttt ctggcatcca
    11281 aaaggggcaa ttattagaaa aacaattgaa gatttctgga aagaaattca tctgaaaaat
    11341 ggttatcaac ttctttatac accacatata gcagattcta ctttatggga aaagtcaggg
    11401 cattattctt tttataagga ctatatgttt cctgtaatgt atatggatga aaataaaaaa
    11461 tttcaattaa aaccaatgaa ttgtccattt catataatta tttatcagac aaaaacaaga
    11521 agttataaag aacttccttt aaggtgggct gaattgggaa ctgtttatag atatgaaaaa
    11581 cagggtgttt tacatggact tttgagagta agaggtttta ctcaggatga tgcccatatt
    11641 ttctgtacag ataaacaatt ggaaaatgaa ataaaggaaa ttttagacct tgcttttttc
    11701 tttcttaaaa gttttggatt ttctgatttt agtgtttttc tctcaacaag accagagaag
    11761 tttgtaggaa caatagagaa ttgggaatta gcaacgaaaa cacttgaaaa atcattaaaa
    11821 gagaaaggaa taaattatga aattgaccct ggtgaaggtg ttttttatgg tccaaaaata
    11881 gatataaaaa taaatgattg tttaggaaga caatggcaat gttcaacaat ccaggttgat
    11941 tttaatattc ctgaaaaatt tgaaataaaa tttattgatg aagatggaaa agaaaaaaca
    12001 cctattttaa tacatagagc aatacttggt tctcttgaaa gattttttgg agttcttatt
    12061 gaacattata aaggaaattt tccattatgg cttgcgcctg aacaattgag aatattgcca
    12121 attacagaaa aacataatca attttcagaa acaatagaag aaaaactatc tgaaaatttc
    12181 agggtatcag ttgatttaag aaacgagaaa attacaaaaa aaataaaaga tgctgaagat
    12241 gaaaaaattc catatatgct tatagttggg gataaagaaa tccaaacagg caattttgca
    12301 ttaagaaaac accaaaaagg tatgcttggg aatttcacac ttaaagaaat tgaagagaac
    12361 tttaaaaatg aaattgagaa aaaattgttt tgatatttac aatattgtaa aatttatata
    12421 taattaaata ggaaaattaa atataagtta gtaaagtttg ttagacaagg tacaaaaaga
    12481 cagaaagaat ttactctttc ataaagtgga gagtgtttct tgttttagtg gacatgaaag
    12541 agatgtaggg ttagagggta gtttatcttc taatgaattg taaattaaat aaatagtaaa
    12601 agggagtaag atatcagaaa ggatagattt caggataaaa ggtatagagt aaattataaa
    12661 ataagtgctc caaaggttaa tcttattgga agtgatggga agcaatttgg aattgttagt
    12721 agagatgaag caataaagat agcaaatgag atgaatttgg atgttgttga aattgttcca
    12781 aatgctaacc caccggtttg taggattata gattttaaga aattccttta tgaacagaaa
    12841 aagaaagaaa aagaaataaa gaaaaaacaa aaaggaattg aagtaaaaga aataagattt
    12901 acaccaggaa caagtgagca tgattataag tttaaaaaac aacatattga gaatttttta
    12961 aaagaaggaa atagggtaag ggttattata ttttttaaag gaagggcaat tttacataag
    13021 gaaaaaggat atgaattact tgaaaaatta acaaatgatt taagagatat tggaaaggta
    13081 gaaatgagtc ccaaaatgga aggaaatagg ttaagtgtga cttttattcc tctttaaaag
    13141 gagtgggtat gccaaaattg aaaacaaaaa aatcagttgc taaaaggttt aaaattacag
    13201 gaacaggtaa ggttatgcat tatggagcag gaggaagtca tcttctctca aataaaagca
    13261 gaaagaggaa aaaaagaatt aaaaaaattc aacaactaaa aaataaggga gatgtaaaaa
    13321 caataaaaaa gttattaccg tataaatagg agaattatat gccaagaaca aaaaatgcac
    13381 ctaattccag aaaaagaaga aaaaaagttc ttaaaagagc aaagggatat aaacaactca
    13441 ggtcaaaaag ttatagaaga gcaaaagaat tcagtgagaa gggattgaca tatgctttca
    13501 atgatagaaa aaagaaaaaa gggaatttca gaagattatg gataaaaaga atttctgctg
    13561 cctgtcagat gcataatatt tcttatagtg cattcattaa tggtataaaa aaagttggta
    13621 tcaatcttaa tagaaagtca ttatccgaaa ttgcttttta ccagccagat gattttgctt
    13681 taatagttga gaaggtaaaa aaagtgttaa atgaaaaaat ttagaaattt tataattttt
    13741 cttcttttag ttttcctttt ttacataaat attactttta ctcttggaga aacattttcc
    13801 tcaatttttt atctcatttc ttttgatatt tctttttctg tttttattat tattgaagta
    13861 ttttattcat tccaactttt agacaacata agaaatttac aaatagagat ctcaaaatta
    13921 aacctgtctt taggtgatta ccaattagaa ggaatacttc taacaacaat tatgtctcta
    13981 ttagaaacag aagaattaaa agttgaagaa gttattgaaa aaattgctga atcagtaaaa
    14041 agtatattta aggatgaaat tgtggtaatt tatttatttg gaggtagatt tttaaaaaaa
    14101 gtaatagggg aggatataat tgaattagaa gaattggaag aaataagtat aaaaaacaaa
    14161 ccacttctca taaacaatac atcttctttt attatgtata aaaatttaag taataaagga
    14221 attacatctt ttatactcac tccattttat acaagaaaag ttgtttcagg agttcttggt
    14281 gttttttcga aaaataatag gaaatttact cttaaagagt tgggactttt aagaatggta
    14341 tctgctccaa catctttggt aattgaaaat gctgaattat ttgagaaaac aaaaattctt
    14401 tcaattactg actcattaac atctctttac aatagaagat tttttgaaac aaaattagag
    14461 gaaatttttt taaaagcaaa ggcagaacaa aaaaaatttt ctttatgtat atgtgatatt
    14521 gattatttta aatattataa tgatacaaat ggccacccaa tgggtgatat tgttttaaaa
    14581 aatgttgcag agataattaa aaatggagta aaaggtggtg atattgttgc aagatatgga
    14641 ggagaagaat ttgtaattat tttccctgat acagaaaaag aaaatgcaat aaaggtttgt
    14701 gaaaaattaa gaaaaaaaat agaagaatat aagttccctt atgagaaaaa ccagccaaat
    14761 ggtgatttaa ctgtaagttt tggagttgct acttttcctg atgatgcttt aattcccgat
    14821 gaattattaa aaaaagcaga tgattattta tataaggcaa aagaaatggg gagaaacaaa
    14881 gttatcagtg catgaaaact tcatctttaa gtgttttcaa tgcatcttct attactttat
    14941 caattttatt ttctgttgac ttttttatgt cttctatcat tttattagtc atatcttcta
    15001 ttttttttaa ctcttgttct ttttctttct cttttgcttc tttatagttt tcaatttctt
    15061 tttttaataa tttctctttt tcctgtctga atttctcttc catatcctgt atgttctctt
    15121 ttatttttat tccttcttct tccgctcttt ttattatatc ttctgcattt tcttctgctt
    15181 cttttatttt ttcaatttta tccattacca tttttttctc cttactttgt tgaaggttct
    15241 gccaaaattt ttgttatctg ttaatttccc cctcactctc gccctctccc ctatggggag
    15301 agggtatagg tgaggggcga tttcatctct ttttcatcta ttttttgaca ctactttgtt
    15361 gaattatacc ttataattga gaaattttta aagttccatt ttccagttca attattacgc
    15421 atccgtcttc aagaaagtaa ttttcaaaag ttctttttat aatgtcatat ttttttcttc
    15481 ttgccgggag aattgtaaga acactatcca attcatttaa tgaatcaaca attttttttc
    15541 ttttcttata gctgttaaga tagaaaattc tctgttttcc ttcaatttct tcttcaagag
    15601 aaagagcaag aaaaatatct aaaatttctt tttttattgt aaaattcttt cctctttttt
    15661 ctatttcata accatataaa atttcctttc tttcatctga attattattc tctgattccc
    15721 agacattttt tacagataca tattttttaa gctgatttaa agttcctgtg tggtttaatt
    15781 gattttcttc tgtataaatt aaagtaattt cttttaattt ttgctttttt ataaaatcca
    15841 ataaagaatt taaaaaattt ctgtccttga aatcatcagg agaaaaaagg accccttttt
    15901 cttcttcttt tattaagaaa caaggttttt tatatgaaaa gaaatatatc tttttacttc
    15961 tatctggtag attatattga taaaatgaaa aagataaaga aagtaaaaaa acaaaaataa
    16021 ggacatcctt tatttttttg ttgtttttta aaagagccca tataagaaat gagtataatg
    16081 taattaccaa aggaattggg aaaaaacaat ttaattcagg ggtatataaa gcaaatgttt
    16141 gcattaaatt taagaaagta tttgctaaaa atccagaaat ttgaaaacca aaaggaaaaa
    16201 taaaagaaaa taaggaaatg agaataagaa ctactgaaag tggaattaaa aaaatgtttg
    16261 aaagaaaacc gataaaataa aatttaccaa aattgtaaag aattattggt agagaagcaa
    16321 tttgggcaaa agttgtagtt actatgaaag taagaaatgg tttttttata aaattaaatt
    16381 ttctcacagt gtaaattatt gtaaaagttg taataaaaga aagtaaaaag gaaagattaa
    16441 aaagagaaat tgggtttaaa attaacataa gaaaagcagc aataaaaatt gaatcaggtc
    16501 cctcaatatc tctttcaaat atctcaccca atagaaaaaa agtgaacatc aatgttgctc
    16561 taataatagg aattcttaat ccagtaataa gagcataact ccatagaaaa ataagggcaa
    16621 aggaagggaa aattttggga tgtttattat ttaattgagc aaatgggata aaaataattc
    16681 ttaaaaaaag aaaaatgtaa gaaagatgaa gtcctgaaat aacaagtaaa tgataagaac
    16741 cacttttaac acccagtgtt cttaaaaaat atggaatttt atcttttcca attactattg
    16801 ttttaagtaa ttctctttct tctggtttat atggtagata cctttctatt ttttcttctc
    16861 cactttttct aatttttgca atacttctgt aaaaaatatt tttaaatcca atttttctta
    16921 aatagtttgc tttcaatgtg taaaagacac cctgcctgtt ataatatttt ttgctgtcaa
    16981 aatcccatgg atttttcttc tctgctattt tctcaatttt tacatctttt atctcaataa
    17041 tttctccttt ttcaaggttt gccgggactt ttaattctaa aataaattta tctccattat
    17101 tttcaactat aaatgatgaa gaaaaaggag aaattttatt aagagagaca acacaacatt
    17161 taaggtgaga taatttttta ttatcaaaat tgaggtcatt aaaactatct tttttaattc
    17221 ttgaagtaga aagcagtccg catataaaaa ataaaatagc aaatattaaa gtttttcttt
    17281 ttgtaaaaat agaaatgaaa agaatcaaga ataaataaaa aaaactaaaa ttgaaattta
    17341 atccaagtaa aataccagaa attagagata taaaatatat taactttacc aattttcact
    17401 ctaatgtttt tcaccaatca atttttcaaa ataatgtcct ttaaaagttg ttataacttt
    17461 cccctgcagg tatatttcag aaaaattctt gtcaaaggaa atagttaaaa tttctccaga
    17521 ccttgctatt attttagtgg gcatttttgt atacccaaga agataactta taattgcaga
    17581 agcaacagaa ccagtcccac atgaaagtgt ttcatcttct acgcctcttt cataggtcct
    17641 tattttaatt gttttttcat tttcaggagt aatccaatca acatttgtcc cttcgggaga
    17701 aaaaattttg tggtatctta tatttcttga atcatttata atatcaatct tatcagtatt
    17761 atctgtaatt ataacaaaat gaggaactcc tgtatttata aaatgccctt ttattttttt
    17821 attttccagg ttcacttcaa tatttaattt ataattacca attattttta actctgcctt
    17881 tatttcttct cctattattt cgcctcttaa aataccactt tttgtttcaa tagttaattt
    17941 cttttttctt tttattcctt catcataagc aaattttatt atgcatctca acccatttcc
    18001 acacatttct ggttctgaac catcaggatt gaaaattctc atcttaaagt cagcgtctgt
    18061 tgaattttca agtaataaaa gtccgtctgc tccaacacca aacttcctat cacaaacact
    18121 tatagcgaat ttatttcttt ctttgattaa aaacttccta ttatctataa caacaaaatc
    18181 attaccagag ccaacaattt ttgaaaattt tatttttctc atattttcat agtttacaaa
    18241 aacatccttt ctctgtcaat ttaacgaatg tgtagaaaaa tacaaaaaaa attatctatc
    18301 acttgtaaag aggttagaag aaaataaaaa ggcattatta aatatattaa aattaccata
    18361 tagacataga ataagtgtaa ggagtacaaa tttaatagag aggttatttg tagtggaaag
    18421 gaggaggaca aaaataatac cgcaattttt gacagaacag agttgtttaa agttggtatt
    18481 cagtgtatta tggagagata gttataaatg gcaggaaata ccgatgtccg caggatcccc
    18541 tcagcccaga gaaattctgt ggaccctacg ggcgagcatg tggatatttt tataattttt
    18601 gtagttaaaa attttatgga tggttgttta ttgtttttac agggaaaaat agacattacc
    18661 cactaggtta tttattagaa attactggaa ttaaaggatt ttatttacct aagtgttgtg
    18721 aagttgcaac agtaattgtt ctataatcat ttttatcaac tgcacagtaa aaatggtaaa
    18781 gaattccttt atataaaatt acgcatggtt tatgagcatg tttaccatca atatcttcat
    18841 ccgttcctat atcaagaatt ggttcaggaa ctttttccca ttgtttcaaa tcaaccgaaa
    18901 aagcaattcc atctctagca ttttttttat ctactccaca ataaaacata acccatttat
    18961 ctttatactt taccagataa gggtctccga ctactaaatc ccaattattc gcattatttc
    19021 tgttaattac tggattatta gaatatctta cccatgtttt taaatcttta gaagtagcaa
    19081 aacctatttc ttctatccaa tcaccactaa aagcaggttt ggcattataa aaaagataaa
    19141 atatcccttt ttctttcata aggaatgttc taaaaattcc tccctgttcc catttcattc
    19201 catcagaagg aaccataaca ggattgaaat ctgctttttc ccagttaatt aaattatcag
    19261 agtatacaat tcctacagaa ccaggccctt tttcatatcc tttttcagga taacctgtat
    19321 aggttgccca atattttcct tgccatttca taggaattgg taattcacct aattgattat
    19381 tttcaagaag aattgataga gcagcagcac cataactatc aaatcttcct ttttcacccc
    19441 aatccaaaat cattctttct cttttccagt ttattaattc atcactagaa gcaagtcctg
    19501 aacgatatcc ttgaccatca aatcctatat aacacatata aaatttatgt tcataccaga
    19561 aaataaatgg acaatctacc atgtctttat caaaatcccc ttctttacca gttggctcaa
    19621 gaattggtat acctaattta tacggtgtta ataaatcttg aattgctttt ttcatattta
    19681 ttttttataa acttaaattg cgaaattcta ttttataatt tagcaagttt cctacttcaa
    19741 aactatttgt tatatgcata aaaggtattt ttaattgcac ttaaaaatct ttctttcaaa
    19801 ttatcttcca tatgtaaatt ataacatttt aaaaatttca catatagttc tttactaaat
    19861 gtgttgagaa ataaataaaa aattgaaatt taaggtgttt ttttataaaa ttattatagg
    19921 gaaataaaat gagagttgta atacaaagag tgaaaaaggc atctttatat attgaaaata
    19981 gttataaggg gtcaattgat aaaggggtgg ttgttcttgt tgggtttaaa aaaggcgata
    20041 gtgagagtga ggtaaaatat ataagcgata agatatttaa tttaagaatt tttgaagatg
    20101 aaaatggcaa aatgaattat ccaattggaa aaataaatgg agatataatg cttgttcctc
    20161 aattcacgct ttatggagat gtgaaaaatt caaatagacc tgatttcagt aatgcagaaa
    20221 atttttcaaa agcaaacaca ctctttgata tatttaaaaa tgaaattaga aaaacaggac
    20281 ttaatctttt atgtggtgaa tttggcaagg aaatgcttgt tgaaatacat aatgacgggc
    20341 cagttacaat aataatagat aaataaatat gagaaataat tataagaatg aaaaagttgt
    20401 tgttgatact tctactaatt gggtttatat tgggatattg aaagacgagg ataatactta
    20461 ttatattctt gatgatgcag atgcttttga cataagtgag acatctttaa gtaaaaatga
    20521 atatctaatg atggtaaaaa aagatgggat tgtaccaaat agaaaaaaag taaaaattct
    20581 aaaattaaaa gttgttgcaa ttactctttt gtctgatata cttgaaaaat aatggaaaaa
    20641 ttacttgcta ttttttttgg ttttatttct ggtatgattg tttattcagg tgttggattt
    20701 tactttacaa ggaaaattga attagccaaa aagtataaat ttaggttaat tgttttttct
    20761 attattttgg tggtctcgtt aattttaaaa gcagtaataa aatgaataga aaagaaagga
    20821 tgattttcag aaaaaattta tacaaaaaaa ttttaaaaaa gaaaaatgac tattatgaag
    20881 ttttagttca attatccaat atttattcta tcttaaattt ttataaaaaa gcacttgaaa
    20941 tagataaaaa aattgttgaa ctttccccat ctgacccaat tgcctgttat aaccttgctt
    21001 gtgattattc tataattggc gatattaaaa aaagtgtgaa atggttaaaa aatgctataa
    21061 attatgggta tgatgacttt gaatatatgg aaaacgaccc tgaccttgaa aatttaagaa
    21121 aatcaggaat tttcaaatgt atttttaata aaaataatta aaatgagaaa ttttttttac
    21181 atatgtgcaa aattacttgg attttctttg tttaaaattc tctttgattg ggaaattaaa
    21241 gggaaagaaa acataccaag aaaaggtggg tttataattg cttcaaatca tttaagttat
    21301 ttggacccac ctattattgg aattgcctct ccaaggaaag ttcattattt ggcaaaatcg
    21361 tctctttttc agattcctgt tttaaatttt cttattaaaa tttatggagc aatacctatt
    21421 gagagagaaa aagaatattc aatttccata agaaaaggta tagagatatt aaaaaaagga
    21481 gagggacttg ttattttccc agaaggaacg agaaatccag agggaaatat agaaatgccc
    21541 aaaaaggggg tatcttttct tgcatataag acaggggttc ctgtaattcc aacaaaatta
    21601 aaaggaacag aaaaagcact ccctaagggc aagaaatttc taaaaccagc aaaagtgaaa
    21661 gtaatttttg gctcatcaat aaaagttgaa aataatgatt atataaatca ggcaaataga
    21721 ataatgaaaa taataaagga actaaattga aaaaaataat tattgcaaaa gatattggtt
    21781 tttgttttgg agttaaaaga gcagttaaaa ttgcagagga aatacttgaa aaaaagggaa
    21841 aattggcaag tttgggagat attgttcata acccttttgt tatggagcaa ttgataaaaa
    21901 aggggttgaa agtttacagg gaagaaaaag aaattaaaaa tgttcctttc ataataaggt
    21961 ctcatggact gcctgtatca aaaataaaaa cgcttgaaaa aagacaaatt gaaatatatg
    22021 atgctacctg tccatatgtt aaaaaaattc attatttgat agaaaatttg gacaaagaaa
    22081 atttttttat tataataata ggaaataagt tccatccaga agttatatct ttaaaggact
    22141 atggtagaaa tatatttgtt cttgatgaag aaataaaaaa ttgtaataaa aaatttgaaa
    22201 aagttgctat aattgggcag acaactttat cttttaaaaa gtatcttgaa cttggtggga
    22261 agttaattaa aaaaattgaa agtaaaaaaa caatagttta taatactatt tgtaaagtca
    22321 cagaaaatag acagaaacaa gcaactgaaa taagtagaga agttgatgct gtttttattc
    22381 ttggtggtaa aagcagttca aatacaacaa aattatatga gttatgtaaa ttgaataata
    22441 aaagaagttt tcacattgaa aatttagaag aaattgataa aatagattta aaaaaattta
    22501 attcaatagg aattgtttcg ggaacatcaa ctccagagga atttatagat agagttttaa
    22561 attatctcaa aaaaaatgat tttaaggagg ttatttaaat gaagaatgaa gtgagtaaat
    22621 tacttgaaga aaaaatttca agtttcaaac caggttcttt agtgaaagga accgttgtaa
    22681 ggatttcaaa tgatgaggta ataattgata ttggatataa gtcagaaggt gctgctccaa
    22741 gagaagaatt taaaggcatg caagatgatg tcaaagaggg tatggaagtt atgatagttg
    22801 ttgagtcatt ggaccccgat tcaaatggac ttattcctct ttcaaaggaa aaggcagata
    22861 taacattgaa ttgggaaaat attgaaaaac attttaatga agataaacca attgaagggg
    22921 tgatttttaa aagagttaaa ggtggttata gagttgatat aggtgtgact gctttcttgc
    22981 caaaatcaca gacagatata aaaccaattg cagacccgaa tgaatgtctt gaaacaaaat
    23041 catattttaa aattattaaa tatgacccat taagaaaaaa tattgttgtt tcaaagaaaa
    23101 aatatttaga agaggagaga gaaaaagaga aatttgattt tctaaaatcg ctggagaaaa
    23161 atcaattagt tacaggagta attagaaata tagtggatta cggtgcattt atagaaattg
    23221 ataatggggt tgttggactc ctacatataa atgatatgag ttggggaaga ataagccatc
    23281 catcacaact ttttggaatt ggggaaaaaa ttgaagtagt tgttactaat gttgaccttg
    23341 ataaacagat tgtttctttt gggcttaaac aaaaaacacc aaatccatgg gaaaatattg
    23401 aggagaaata tcctgttggt tcagttgttg aaggaaaagt tgtaaatata actgagtatg
    23461 gtgcttttat aaaaattgac gaaggaattg aaggactttt acatatttct gaactttcat
    23521 ggactggtag aataaaacat ccttcagaaa ttgttgcaat gggagataca gttaaagtga
    23581 aagttgttga tataaaaaag gaccagcaga aaatttcttt cagtttgaaa caattagaac
    23641 caaatccctg gcctgaaata gcaaaaaaat acaaagttgg aactgttgtt aaggggaaag
    23701 tttataatat aactgattat ggtgctttta ttgaaataga agaagggata gacgggcttt
    23761 tgcatatttc aaatatttca gaccagccaa taaaacatcc atcagaggtt ttaagaaaag
    23821 ggcaaaaaat agaaacaatg gttcttgaaa ttaattctga tgagaaaaaa ataaatttag
    23881 ggttaaaaca attaggtgaa attgtaaaaa aaggagaaga aaatgagagt gatagtgaag
    23941 agtaattatg aagaaatgag tgaagaggca gcaaagatag ttgcagaact tgtaagggaa
    24001 aaaggtgatt gtgttttagg acttgcaaca ggtagtactc ctgttggcct ttatcaccaa
    24061 cttgttagaa tgcacaaaga agaaggactt gatttttcaa aagttaagac attcaattta
    24121 gatgaatatt atggaatttc tccccaacat ccccaaagtt atagatattt tatgaatgaa
    24181 aaccttttta atggaataaa tatagataaa aaaaatacat ttgtccctga tggaactatt
    24241 ccacttgaag aaattgagaa gtattgtgat gaatatgaaa aaatgataaa aaaagcaggg
    24301 ggaatagacc ttcaggttct tggtattggg agtgatggac atattgcttt taatgaacca
    24361 gggtctcctc tttcttcaag aacacgcctt gttgcactgg ataagcagac aatagaagat
    24421 aattcaagat tttttgataa tatagaagat gttcctaaat ttgcactctc tatgggtgtt
    24481 ggaacaattc ttgaagcaaa agagatattg tttcttgtaa atggagaaaa aaaagcggaa
    24541 gttgtggcaa aagcaattga aggaccaata acatctctta ttactgcttc atctttacaa
    24601 tgtcaccaaa aggtaaccac tatattggat gaagcagcag caagcaaatt aaaaagaatt
    24661 gaatattata aatttaaaac agaatcagag aaaaaaataa acaaaaaatt attttaaaaa
    24721 tgggacttat tgaaaatttt atagagaaag caaaaagaaa taaaaaaagg gttgtatttc
    24781 cagaaggaga agaagaaaga ttaattgaaa gtgctataaa attagaagaa gatggtatag
    24841 caaggccaat acttgtaggt agaagaaata tcataaaaga gaagattgag gcactaaaaa
    24901 ttccttctga taaaattgaa attgtagata ttgaaaataa tccttatatt gatgaatttg
    24961 caaatgttta ttctgaaaaa agaaaaataa aactatccat tgctcaacga cttattaaga
    25021 gggagttaat ttttggtggt atgatgcttt taacagataa agttgattta atgattgcag
    25081 gggcagcaaa tacaactgcc tctgttattc aagcatcttc tttaacagtt gggtataaac
    25141 aagatacatc aaccccatca agttttttta taatggtctt gcctgataat aagatttatt
    25201 tttatgctga ttgtgctgta aatattgacc caacttatga acaacttgca gatattggta
    25261 tatcaactgg tgaaaatttt gaatgtctga ttggagaaaa agcgaaagtt gcttttctct
    25321 cattttcaac aaaaggatct gcctcacatc cgtttgttga gaaggtccag aaggcagtag
    25381 aaatagcaaa aagtaaaaat agaaattttc ctgttgatgg agaatttcaa gcagatactg
    25441 ctattgtttc ggttgttgca gaaaaaaaat tgaaagaacc atcagaagtt gcaggaaaag
    25501 caaatgtttt aatttttcct gaccttaatt ctggaaatat atcttataaa ctcactcaat
    25561 atcttgcaaa agcacaggca ttgggcccaa ttttacaggg atttgctaag ccagtaagtg
    25621 acctgtcaag aggggcaaag gttgaagata ttataaaaac gaccgcaatt ttgtcctgta
    25681 tgtgttgaat aaaaaaagtg taaaatgtaa agaggcaaat gcaaatttaa agtgtaaagt
    25741 aaggaaagaa tagaaagtag gaaaagaaaa tttaatataa tccttcacct attttcttcc
    25801 caaatgaaaa gaaaaaggag agaaaggaga aaaaaatgaa aattttaata tttgaagatg
    25861 aaaattggaa gaatttatta cctttaagtt atataaaaaa tacatttgaa ttaagatgtg
    25921 gttattatac tttatttgag agaattgctc aaaattttaa tggtgaaaag ggactatggg
    25981 ttagagatta tatttcttct cttacaaaaa ttaaattcaa tttacccaca aatgatgaaa
    26041 attttataaa agatgatatt tttattataa atgggacaac aataattgat gggaaagtaa
    26101 atattgaaga agaaaaaatt tttgttgatg aatataataa tatcctttat ggatatataa
    26161 aaaaagaaac agttgaaaaa ttctgggaag gaaatttctg gaaatttctt tctaatttaa
    26221 aagaaaatat aaaaaaagaa aaagtagaac ttatttctat aaaatatccc tggaatttaa
    26281 tacattttaa tgggcaactt ttaaaaactg attttgaaaa tatacagaat aaaaaaattg
    26341 aaggtgaagt ttccggaaaa tgtgaaataa taaatgagaa aaatgttgtt ataagaaatg
    26401 aggcaaaaat acatccattt gttgttcttg atgcatcaaa aggaccaatt tttattgata
    26461 gagaagcaga aattttccca tttgcaagaa ttgaaggacc atcttttatt ggagaaaaaa
    26521 cacaaattat gccaggagca aatataagag agggaaatag ttttggtcct gtttgcagga
    26581 ttggtggtga agtagaagaa agtatttttc attcctattc aaataaatat catgatggat
    26641 ttattgggca tagttatatt ggggagtttg taaatttagg tgcattaact acaaatagtg
    26701 accttaaaaa tgattatagc gatgtagttg tttatctttg tggtagccca acaaatacag
    26761 gtgatttaaa agttggctct tttataggag accatacaaa aacaagtata gggacattat
    26821 ttaatacagg aacaattatt ggtattatgt gtaatattac tgcgggtgga atattgccaa
    26881 aatattttcc ttcttttacc tggtatataa acaataaatt tatgaaagga acaggtattg
    26941 aaacaggtat agaaacaaca aaaaaggcaa tggcaagaag aggaagggac ttaccaaaag
    27001 aggaagaaga agttataaga tatttatatc aagaaacaga gacagaaaga aaagaatatg
    27061 taaaaagaag cagaaagaaa caataacagg gagaaaaaat ggaagaaaaa gaaattgaag
    27121 aaagagcaaa aaaagttgta tcttctattc ttggtgtaga tgaggaaaaa ataaaaagcg
    27181 aaagcaggtt tgttgaagac cttggagcag aaagtgtggc aagtttggaa cttgttgctg
    27241 gttttgaaga agaatttgat atagaaatgg atgaaaatgc agcacttgaa gttaaaacat
    27301 ttggtgatgc agtgaagttc attaaaaaat atatagaggg aaaatgaaag ttcttgtatt
    27361 taattgtgga agttcctcag ttaaatttaa gttatttgaa atgcctgagg aaaaacaaat
    27421 tgttgaaggt tctgttgaaa aaattggtga agagatttca ttttttacat taaaaaaaga
    27481 aagtgaagca aataaagaag aacttaaaat aagaaatcat ttagaggcat tacaacttat
    27541 agcaaaatcc cttgtagata aaaaaaacga aattatttct aatttagacg aaatagaagg
    27601 agttggtcat agagttgttc atggtggaga aggttttgat aaaagtgttg taatagatga
    27661 ggcagttata aaaaaaattg aagagtatca gggacttgct cctttacaca atcctcataa
    27721 tcttgctgga ataaaagcag ggatgaaatt tttccccaaa tcagtacagg tatcttcttt
    27781 tgatactgct tttcattcta caattccaga agttgcttat atttatggga taccatataa
    27841 gttttatgaa aaatatcaac tgagaagata tggttttcat gggacttctc atagatatgt
    27901 agcaagaaga acaagtgaga taatgggaaa aggaaaatat gatataaatg ttataacatg
    27961 ccacctggga aatggttgca gtgtaactgc gtgtaaaaat gggaaaagcg ttgatacaag
    28021 tatgggatta acaccattag aaggacttat tatgggaacc aggtctgggg atattgaccc
    28081 tggaattata ttttatttag gggaaagtaa tggtctttcg ttttctgaaa taaacaaaat
    28141 ttttaataaa gaaagtggtc tacttggaat atctggtgtc tcaaatgatt ttagaaattt
    28201 aatggaaaat attgaaaatc caagagtaaa acttgcaatt gaaattttct gctaccgact
    28261 caaaaaatat attggttctt atattgctgt cttaggtaaa atagatgcta tttcttttac
    28321 tgctggaata ggtgaaaatg tcccttatgt aagagaaaaa tcacttgaaa atcttgagaa
    28381 tttaggaatt gaaatagata aagaaaaaaa caaaaaaatt actgaaagaa aagaaggaga
    28441 aataagtaaa gaaagttcaa aaataaaggt ttttgtaata cctacaaatg aagaattaag
    28501 aatcgcttat gatacatatc aacttacaca aaaaacaaaa ttatgaacaa aaaatatatt
    28561 gttattactg gaggagcagg ttttatagga agtaaccttg ttcaatatct aaatcaaaaa
    28621 ggaatagaaa atataataat tgttgaccat cttacaaatg aaaaatggaa aaatttaatt
    28681 ggtaagaaat ttattgatta cattgaaaaa aataaatttt tgaagttgat ttctgaggga
    28741 ccctatggag aaaataataa actaccattt gagattgaga aatgtgttca tttaggtgca
    28801 tgcacttcaa caattgagaa aaatgtgaat tatgtaataa gaaataattt cctttataca
    28861 aaaaaattgg ctgaatattg tttttcaaaa ggaatatatt ttatttatgc ttcatctgct
    28921 gctacctatg gaaatggaga aaatggtttt tctgatgatg aggagaatat tttttcttta
    28981 aaaccattaa atatatatgg attttcaaaa catttatttg atttaattat ggttaaaaat
    29041 gatttatgga gaagaaattt ttgtggtctt aaatttttta atgtttatgg accaaatgaa
    29101 taccataaag gagaaatgaa aagcgtaata aacaaaatgt actatcaaat aatagaaaat
    29161 ggatatgtta cactttttaa atcatataga gacgattaca aaaacggtga acaaaagagg
    29221 gactttattt atgtaaaaga tgtagtcgat gtaatatatt ttttcctcca gaataaaaag
    29281 tgtggaattt ataatgtcgg aactggcaaa ggtgaaagtt ttaataaagt ggcagaagta
    29341 ttatttactt tactgaaaaa agagaaaaaa ataaagtata tagatatgcc agttgaaata
    29401 aggaaaaatt atcaatattt taccgaaggc aatatctcaa aattaagaag tgctggatat
    29461 agtaaagaat ttacttctat tgaagaaggt atttctgatt atttgaaata tcttgaaaat
    29521 ggaatttatg aatgaaaaaa tagcaatagt tgtaaaattt tctctcatct ctcaaaaaat
    29581 ttcttccaat ctttttataa aagaggagca ttttatcttt tccaaaatta taaaaaggga
    29641 tgatttacta ataattttaa caaatttctt tcaaagggta taatttaact tatggggaaa
    29701 attatttttg ttggtgaagc aacatctatt gaatttttta agaattttgg atttgaaata
    29761 ataccagcga cttcaaaaga agaagcaaag gaaattcttg ataagaagaa ttatcaagat
    29821 atagaggtac tatttattac agaagaaatt tttgatagtg ttgttttttc aagatttgtt
    29881 gatgagaaaa aaattgtagt tataccaagt ttaaaatcaa ataagggaaa gggttataag
    29941 atagttgaaa gattgataag taaagcaaca gggatgaaag gagagtagaa atggtaaagg
    30001 gagaaataat aaaggtctca ggacctcttg tagttgcaaa aggaatggaa aaatcaaaaa
    30061 tgtttgatgt tgtgagggtt ggggatgaga aacttgttgg agaaataatt aaaatagaaa
    30121 atgggattgc ttctattcag gtttatgaag aaacagaagg gttaaaagtt ggagaccctg
    30181 ttataagtac tgaactacca ttaactgttg aacttgctcc tggacttctt tccacaattt
    30241 ttgatggaat tcaaaggcct ctttctgaaa ttgagaaaag gggtgatttt attagaagag
    30301 ggattgatgt tccttctatt gatagaaaca agaaatggga ttttaaacca gttgttaaaa
    30361 aaggagatat agttgaaggt ggggatgtaa taggtgctgt tcaggaaacg ccacttattg
    30421 ttcataaaat tatggtccca cctgacctaa aaggggaaat agttgaaata aaagaagggt
    30481 cttttactgt tgaagaagaa attgctaaat taaagacgga aaatggatat gtctctctta
    30541 aaatgcttca aaaatggact gtaagaggaa aaagaccatt taagaaaaga ttaccaccag
    30601 atacacctct tgtaactgga cagagagtta ttgatacatt atttccaata cttaaaggtg
    30661 gaactgcttg tattccagga ccgtttggct caggaaagac agtagttctt catcaaattg
    30721 caaagtgggc agatagtcaa attgttattt atattggttg tggagaaaga ggaaacgaaa
    30781 tggctgatgt tttaatggaa ttccctgaat taaaagaccc tgcaagtgga agaccattac
    30841 ttgaaaggac tgtgttgata gcaaatacat caaatatgcc tgtggcagca agagaagcaa
    30901 gtatttatac cggggttact attggtgaat atttcagaga tatgggatat tctgttgctt
    30961 taatggctga ctcaacaagc agatgggcag aagcattaag agaaatatca ggaagaatgg
    31021 aagaaatgcc aggagaagaa gggtatcctg catatttagg aacgagggtt gctgaatttt
    31081 atgaaagagg ggggaagatt atatgtttgg gaaaagaaga aatagaaggt tctttatcaa
    31141 ttattggagc agttagtcct cctggtggag accttacaga ccctgtggtt caaatgacat
    31201 taagagttgt taaagttttc tggggacttg atgaccgttt agcatatcag agacattttc
    31261 ctgctataaa ctggcttaca agttattctt tatataatga aaatgttaag gaatatctta
    31321 atagggaaat atccgaggac tttcagaaaa taagaaatga aggtgtctca atccttgaaa
    31381 gggaagacga attacttgaa atagtccgac ttgttggtat ggaaacactt tcagaagaag
    31441 atagattaat tcttgaaaca gcccgttcaa taagagagga ctttttacat cagagtgctt
    31501 ttgatgaaag agatgcttat actactttga aaaaacagta tctgatgctt aaaacaataa
    31561 ttttatttca tagatttgca attgaacaaa taaaaaaagg aaagtccctt tctgatattt
    31621 taaaacatcc agttagagca gatattacaa gaatgagata tataagtgaa aataagttgg
    31681 atgattttaa taatattata aataaaatct cttctataga agaaaagaaa taaaaagtac
    31741 tcttgaaaat aatataagaa aaggaggagg gaaatggcaa ataggtttaa aacatatttt
    31801 ttgcttatac ttttaacagt tctctttgtt tttattggca gttttttcgg taaaagcggg
    31861 atgattattg ctttccttat tgctcttgct cttaactggt tttcttattt tttctctgat
    31921 aaaattgttc ttgctatgta tagggcaaga cctgtttctt ataatgaagc acccgattta
    31981 catggacttg ttgaacaact ttgtcaaagt ggaaatatac caaaaccatc aatttatata
    32041 attccttcac aatccccaaa tgcttttgca actggtaggg acccaaatca tgcagcagtt
    32101 gctgtaacag aaggaattct tgaaattttg aatgaaggtg aacttaaagg tgttttgggg
    32161 catgaactta ctcatgtaaa aaacagggat attcttatcg caacaattgt tgcgactata
    32221 gcaggagcaa taacaatgtt agcaaggatg ttacaatggg cagcaatgtt cggtggagtt
    32281 gatagggata atagaaaagg tggagttttt tctcttatag cgatgcttct ttttgcaatt
    32341 cttgctccaa ttgctgcaat gataattcaa cttgctgttt cccgttcaag agaatattta
    32401 gcagatgagg ggggagcaaa actatgtggt aatccattat atttagcaaa tgctttaaaa
    32461 aaattatcag aaggtgttag aagaaatcca atgacaacat ctaatccctc tacttcacat
    32521 ctttttattg ttaatccatt ttctgctaaa ggtttagtaa atttattttc aactcatcca
    32581 ccaatggaag aaagaataaa acgacttgaa aatatgttag taagatgaat aatgaaaaat
    32641 taaaaaaggt tttgactgat tacaaaaatg gcaaaataag cgaagatgat gtttttcagt
    32701 actttctata tttcccttat aaggatttaa aatttgctaa aattgatact tacagaaatt
    32761 taaaatttgg ttttccagaa gttgtatatg gtaaagagaa aaaattaagt catcttgtaa
    32821 aaattgttga aaatatgatg aaactccatt caaatttcat tattacaaaa gttgatgaaa
    32881 aaattgctaa aaaattaaga aataaatttc cttctcttac ttacttcccg gatgcaaaaa
    32941 ttatgtctgt aaaagaagca aaaattgaca aaaaaaaggg atacatatgt gttgtttgtg
    33001 ctgggacttc tgatatacca atagcagaag aagcggcaat aacagcagaa atacttggaa
    33061 ataaagttga aagaatatat gatgttggtg ttgcaggaat tcatcgtcta ttagataaaa
    33121 ttgaaatttt aaataaagca aaatgtataa ttgtagttgc aggaatggaa ggggctttac
    33181 caggagttgt tgggggtctt gtaggaaaag cagttattgc agtcccgact tctgttggat
    33241 atggaacaaa tttttctgga cttacacccc tttttactat gcttaatagt tgtagtccaa
    33301 atgttgtggt tgtaaatatt gataatggct ttggtgctgg cttttttgct aatttactta
    33361 gttgaggagg aaatgggaat aagtgaaaaa gataaaacaa ttttaagaga tttagcgaag
    33421 aaagtaaaag aaatttctct taacccaatc tggcaagaga aaataaattt gtggaaaaca
    33481 cataataagt taggaaaaac aagaccgatg gttatgattt atcttggaga tggggatgta
    33541 tggaaggata taattccaga aggaactctt ttatgcgaag atgattattt acgaaaaatt
    33601 gagtttaatt taagagaaag aatttataga tgggagaatt taaaagatga tacagttata
    33661 gatgggagaa tttttactcc aatttttgtt gagaatactg gttttggaat tactgaagaa
    33721 acgttaaaac cagaagaata tggaggcgct gtccattacc atgcagttat aaaagaagaa
    33781 aaagatattg aaaaaattaa gaaaccaaaa atttcagttg attataaaag aacagaaaaa
    33841 acatttgaac ttgtttcaaa tatacttgga gatattctaa aaatagaaaa aagagggccg
    33901 tgtggtttct ggtttgcccc aattgatatg tttgcaaaat ggcgtagttt tgaccaatta
    33961 tttattgata tgatagaaag accacaatgg ctccataaag tgttaaattt tataacagag
    34021 tgtacattag aagaaattga gttttatgaa aaagaaaata ttcttggttt taatcatgat
    34081 ttaaattatg aaggttctgg tggtgctggt tatacagatg aattacctca aaatgatttt
    34141 gatgggaaac atgtcagaac aaaagatttg tggggacatg cagcaactca aattttttct
    34201 cttgtttctc cttcaatgca cgaagaattt gcaataatgt atgaaaaaag attactttca
    34261 aaatttggtc tttcaagtta tggttgttgt gaaccactcc ataagaaagt agatatactt
    34321 tttaagaaca ttcccaatat taggagaatt tcaatgagtc catgggtaga tataaaagaa
    34381 ggagcaaaaa acattgaaga taaagcgatt ttttcttgga agccaagtcc ttcaatactt
    34441 gcgggggaaa tatttgaacc agaaaatgta agaagaataa taagagatgg attagaaaaa
    34501 acaaatgact gtgttgttga aataattatg aaagatgtaa gaacctgtcg ggaaaaaccc
    34561 gaaaggttaa ctgaatgggt gaatataaca atggaagaag caataaaata tctaaaataa
    34621 aaattattca aattatcttt gtaatctttc tattaggtat tcaaaaaatt gctcaagaat
    34681 ttctggtttt atatttggtg aaagatgatt cccaactgca aaaattccac cttttgtttc
    34741 aaaaaatttt tcaattgttc tatcaatatc ttttttcacc ttttcaattc tcccaaaaac
    34801 taggtctctg caatcaacat aactaccgac aagacaacat tttttgccaa aattttcaaa
    34861 catatatccg aaatcattac atggttcaaa aatcaatcca tctgctcctg cttctacaat
    34921 atcttccgct aattgcataa aatttccatc tgaacagaaa agaacttttt tccctttctc
    34981 atgtagaact ttccacagag aaacatatct tggaattata atttttttat aaatttcaag
    35041 tttcataaaa gggccgcttg tccaaacaaa atcatcgtgt tgaataaata cctcaatact
    35101 tgtttctgcc catgcttttg taaaaaacat tgttcttcta aaaaaactat caagaactct
    35161 ttctaattta ccaatatttg atgcagcaag taaaaacatt tcccaaccaa atgcttgaat
    35221 tgctcctgaa acaatggtct tataatatcc acctggaatt aattgttcag ggaattttat
    35281 tttcatatct tcaataatct tttcatattc tttaacctgt ttatcaaaat cagtcagccc
    35341 atactcttca actgcatcaa attcccatac atcttcttct gttctaaaag gtgatttttc
    35401 tttttctctt ttatcacttc catcagaaga atattcagca tgtcccatat cagttatcct
    35461 tcctgttttt ccccagttaa ttaatccatc attagtatga aataaaaaat caatacagag
    35521 tttgttatat gcaatttttt cgaaatcggg gtcattttca gaaagctctg taattttttt
    35581 taaaaattct ttatgatata ctaaactata ttctgtatga gcaattttcc cttttctttt
    35641 aagatttata ttctcccttg ctatttctac actcattttc cctccacatt ttttttagca
    35701 ggaattttga gagtaaattt tgttcctttt ccaatttttg attttacttc aacaatccca
    35761 ttatgttctt taactacttt ttgaactatt gcaagtccaa aaccagttcc tcctgaacct
    35821 tttgttgaga aaaatatatc aaaaatttta tctaaattct gttctggtat tccacagcca
    35881 ttgtcttcaa cccatatttc aattatttct ttttcactgt tatatcttgt tccgactttt
    35941 ataattccct gttttccttc aacagcatca acagcattta gcagaagatt tgtaatacct
    36001 ctttctattt tataagggtc aatatatatt tgtggtatat tctgtccaaa ttcttttttt
    36061 atttttattt tgttttcctt aaagaggtcc ttattttctt ttataattgt atcaagaaga
    36121 ttatttatat ctgtcaattg taattgtaat tctctttcct ttgtataatc aaccattgaa
    36181 agaacaagat ttttcatttt ttcgtaacta ccttttagta tatttactcc atctattatt
    36241 acttctttcc tatgttttcc tttaagtcct gtttctataa gatataaacc gttttcaatt
    36301 ttttggagat ggtttcttat atcatgagaa agtccggata ttactttacc tattgttgca
    36361 agtcgttcat tttttatcat ttcctgagta aggcgcatat tactcattat gaaacttatt
    36421 attgagcagc cagatattaa aattagcgtt tcttcttttg taaaactttt tcttttacca
    36481 atcaatacct gaccaaattt ctcttttgtt gttcttaaag ggactgaaag aatgttatat
    36541 tttttactat ttaaatttac ttttgggaat attaaactct caccttttat aaaactatca
    36601 gatgctaatt gatgaagttt atcaattttt tctttgtcat aaggatatga ataaacagga
    36661 gaaagttctt tttctccaag aagataaata cttataatat cagtttttaa aaactttctt
    36721 attctttctc cgagtatttc tgatagagtt atttcattaa caacttctgc aattttttct
    36781 gttaactggt ttataaaaag aatttcaaca aaaaaagcac gcattccttc ttccatgtct
    36841 tttacctggt caagaagtga ataaacaaaa ttatcaactt ctttaatacc ttcttcatca
    36901 tctcttttta tgattgcatt gaatttatca ataaaaaatg taactggttt aacaatagtt
    36961 gaaactgttt cttttaatct gtctttcttt tccatttcaa atacctattt ttaaaaagtt
    37021 atttgttgca attgatattt ttttcccatc cgagattttt tcaatatagt ttccacctgc
    37081 ttgatttaaa attatttttc caccagcaat atcccatatt tttaggttaa attcaatata
    37141 aaaatcaact ctaccacatg caacatagca taggtcaagt gccgcagagc cagtcatcct
    37201 tatttttttt atttttggag catatttttt aattatttca attccaattt taatttcatc
    37261 atttcctttc ataaatccta ttataccaac tgattctttt aaagttttta tttcacttac
    37321 tttaattgac tttccattta aaaaagcacc tttccctttt attccagtaa atttttcttt
    37381 tttcataaca tcataaacaa taccaaattc ctctttttca cctttacagg caattgaaat
    37441 ggcaaaatga ggtatttttc ttagataatt gaaagttcca tctaatgggt caattatcca
    37501 tatatatcct gttttatttt ttttctctcc tttttcttca ctgacccatc catcatctgg
    37561 gaaatttttt tcaatttctt caagtattat tttttcgctt tcaacatcct gaaatatttt
    37621 tacatcaaac ctgaaatttt ctttttcttt accttttcca aagttttctt ctaaaaattt
    37681 tccacttttt tctattattt tttccgctaa ttgatacctt tccatatttt tctccggatt
    37741 taaaattata ccttattatc ctgtattata aaactgaagt tcataagaaa ctgttgtgtc
    37801 aaataaaaat ctactcgaaa tactaattcg ttgagacatt aaa
//
LOCUS       DAOOVW010000011        37636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_707_length_37636_cov_6.825736, whole genome shotgun sequence.
ACCESSION   DAOOVW010000011 DAOOVW010000000
VERSION     DAOOVW010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 37636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37636
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_707_length_37636_cov_6.825736"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..724)
                     /locus_tag="PLW95_02630"
     CDS             complement(56..724)
                     /locus_tag="PLW95_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOV21561.1"
                     /translation="MFKLFFHTIYCKTNNALCLFLNLFQDQKCYGCDKKEKLSKYGFC
                     QNCEKEIIFYQNSDTLFYHVAAYEGPIKNAIHKFKYGKKKFYGKKLALFVYDNLKEKI
                     PEFDLIIPVPLHWKKEFSRTFNQSAIISVYLGKLFGKDVLIDGVVKTKNTPSQVELSG
                     SEREKNVLNTFKVRKTSSIKDKKILLVDDVFTTGATTNELKKILKKAGAKNVIVITIA
                     KTIL"
     gene            704..1756
                     /locus_tag="PLW95_02635"
     CDS             704..1756
                     /locus_tag="PLW95_02635"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOV21562.1"
                     /translation="MKKQLKHIYISKEFFINFIISFLIFTFIFLINGIFKAIEIIIKG
                     ASFFSVVKVLIYFFLSSLPYIFPLTLLSSSTSVFSRLTHDREIQIFSFSGINPLILTK
                     NLLIFAVLCSILLIYYNLLIFPSIKYQSRKTIYNLTAKNPIAFFQPKTIINDFPDFSI
                     YIGDLSKDLNFKNLSIIQKSDKITLFIKAEEGKINYVPETNNIIFLMKNGFITSQASN
                     KISKLDFSEYTFHIELPESFSFKEPQKKLSELPLSQLKIKNNIDANIEINERISFGIT
                     PLIFVFLGIGIGMKLKNKSRVLCIGVAGATSLFFFLFLILGEIIAKKLNFSLFVYLPV
                     LIFTFITYYLNKFSVR"
     gene            1910..2926
                     /locus_tag="PLW95_02640"
     CDS             1910..2926
                     /locus_tag="PLW95_02640"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOV21563.1"
                     /translation="MVKIKYILFNFFKLFLISSLCLLSLFFIIQILEDLPDIISGSQK
                     FLLSNYILTLPSSFVLISPLITLLSGMFFVSEMVKANEIKIFEISGINSLKIFSMFLF
                     ASLIISSFTFYIKNYVVPKTIKGNEYQTSISFSSPSFLFKSDRYEKDGKFINIEISII
                     LEDGSITSIRAKEGIEKEKNLWTFKEGKFYLIDKNTNLKKEENFNSKTFELPITSEIL
                     LVAGKNPDSIFINQLKSTIKELKKMGFSPFLLETYFNEKIAYPLLNLFILFAIFPFFL
                     SKQKITRFLVLSLTIVIGFLSYGIYSFLIALSKSGKIPPFFGCWGLHLFVFISFLFTL
                     FKFR"
     gene            complement(3071..3493)
                     /locus_tag="PLW95_02645"
                     /pseudo
     CDS             complement(3071..3493)
                     /locus_tag="PLW95_02645"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
     gene            complement(3490..3894)
                     /locus_tag="PLW95_02650"
     CDS             complement(3490..3894)
                     /locus_tag="PLW95_02650"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOV21564.1"
                     /translation="MRKSVNEIKTILKEYFQKHPEIEVAYVFGSVAQEKDNVLSDIDI
                     GIIVDKGQINEQSYRYGYKAEILTDLIKLLKTNNIDLVILNDANTLLKHRVLYFGKVI
                     YSKNEKNRIQFQVNTINKYADFRQFIKQQENK"
     gene            complement(3934..4146)
                     /locus_tag="PLW95_02655"
     CDS             complement(3934..4146)
                     /locus_tag="PLW95_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HOV21565.1"
                     /translation="MENFMNRIEINPKILLGKPIIHMTRIPVELIVKLVAQNWKEKDI
                     LKEYPCLKKEDIRAAFLYAEKVIEEE"
     gene            complement(4658..4807)
                     /locus_tag="PLW95_02660"
     CDS             complement(4658..4807)
                     /locus_tag="PLW95_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21566.1"
                     /translation="MSIPEHLFYFSLKTLKQICEKSGFIYKGSFFRFPWREGIKAVFL
                     KETNL"
     gene            4894..5955
                     /locus_tag="PLW95_02665"
     CDS             4894..5955
                     /locus_tag="PLW95_02665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOV21567.1"
                     /translation="MIKINIILNIGKKPSGAGRPIIEFSNQLYKNNSVVIYKPFNPEY
                     KKGVEYKFREIIGYILKGREYKQKWIECKPKVFIIPKCKEKFIRDADILFFRSPKFLE
                     EIGHFSLSKGKKVMRVSDIFFALNPPKNIPSDIYIITSSTMVKSLLQQKFPDKRIFLV
                     VNGVNLNFFGFPERKPIFNGRIGMVYYSGKNPEHKGMEDGFWVMNMLKEKYPSLEFYL
                     LGWEKEKKIPPFVKFTEGLTSEKVREFYRQIDILIYPSKLDACPNPPMEGMASKCAVI
                     TTNVGGIADFVIPGVTALVNEPGEKAEMLKNAIALIENKKLWEKISNAGYEKIKEFSY
                     EKQGQKLENVFLQILNDKN"
     gene            5973..7157
                     /locus_tag="PLW95_02670"
     CDS             5973..7157
                     /locus_tag="PLW95_02670"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOV21568.1"
                     /translation="MNIGMILEDEFPPDIRVEKEAQVLLSQHFNLHLLSAVKKQEEKR
                     KEYKGILVEQKPIIYPNVYHHLLDKLNFLRGKETLFHKQIEQFVKRNKIEIIHAHDLP
                     VAFVSLKIAKKLGIPIIADLHENYPYYKLIKKRKNTKRFRYWLNIEKKVVKESNFIIT
                     TCEEMRERILSEHCIPSEKIYVVPNYYGGEFENIVVDKSILEKYSQKFMILYSGVVSL
                     HKGIETLIKSMEYLKNYPEIVLCIVGVKKNKEEFEKLAKTLHVEEKIEFIKWQPIEKT
                     YSFYLASSVCSVPFEKNVQTDCSSPHKLFQYMQCKKPVLVSNCNSLIRIVEKSNCGFV
                     FECGHPKDLADKILILYKNKKLRIEMGENSARSVKNNYNFEIAGSQLSLLYNNLTKWF
                     KK"
     gene            7142..8398
                     /locus_tag="PLW95_02675"
     CDS             7142..8398
                     /locus_tag="PLW95_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21569.1"
                     /translation="MVQKMKKVLIITYHFPPSKKVGVLRIKGLAKYLPEFGWEPVFVI
                     PLLQQEPEINSQIIETHPPYQKFFKYYFSKAKKRIKKISLPLPPFHKRVKSFFRGMID
                     IPDNRIGWLPFAYEAGKNLIEKEKINHMISILGPATCHLVGSNLKKRYKNIFWIADFR
                     DPWTLYKNNTLINKYKRNLEIETLKEADAITIVTRKLAYDMKKIHKNKDIYFIPNGFD
                     PEEIDLQPQKLTENFTITHTGSLFINKRSPELLFKALSELIKEQKIKANDISIRFFGK
                     NQNWVLPLAEKYGLKDVVKYYGERERKECLKKQRESQLLLIIQVEGKGEEGCLTGKIF
                     EYLTSFRPILSIGDKDNEVSNLLKETDSGIHCTTVCELKETVLKYYREWEEKGYIGYS
                     GIKEEIMKYSHREMAKKFADILNKNN"
     gene            8399..9106
                     /locus_tag="PLW95_02680"
     CDS             8399..9106
                     /locus_tag="PLW95_02680"
                     /inference="COORDINATES: protein motif:HMM:NF024775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOV21570.1"
                     /translation="MNNIKDIRHRIERFFKQLVGLEVWSKIEVFCPYEMIGNYCVSFL
                     DKINSDSIIYSLGVGRDISFDLDLINKKGVIVHAFDPTPIAKNWIQSKNLPKNFKFYP
                     YGIANFNGSAEFHIPSNPKSTSYSLLDSGKTLGTVKYPVKRLKEVMKELGHTKIDILK
                     IDIEGGEYMVIEDIINSEIKVIQLLVEFHHRYKKIGLYKTKQTIKQLRENGYKIFYIS
                     PDGEEYSFIKYNEIFNK"
     gene            9087..10052
                     /locus_tag="PLW95_02685"
     CDS             9087..10052
                     /locus_tag="PLW95_02685"
                     /inference="COORDINATES: protein motif:HMM:NF021601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HOV21571.1"
                     /translation="MKFSINKKATLCLTLNDFHPNQDDIDRFIKKNKKLSFEGLKFFP
                     ISKFYFQSHKRKSYQYYFIFKKINKIRMAYAQWKGTEIPKGSFKYLIRVDDFPHWEKN
                     YKEFLKFAELFSKYNIPFLLGVTPFLSLNPKSIYNTKFKFLDDNEVKILKEFSNIEIS
                     LHGLTHQSVNKKRTTEFIGLTDGEVEEKLTLGIKLLEEKQIFPIAFIPPFDKIDKNNF
                     EIIKKHFKIICGGFDTAKYVGYLITPCIVDNKTAFVPSYFPLSGNINEIHNFLLDIEE
                     ENIIVPITIHWTYEVNNLERVENFLKFIEKKVISWKDFLSTTENL"
     gene            complement(10060..11082)
                     /locus_tag="PLW95_02690"
     CDS             complement(10060..11082)
                     /locus_tag="PLW95_02690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOV21572.1"
                     /translation="MNILHLPSNISNQPYLFVKGLRQQRINADYLFFKKKKFYEQKGI
                     FDGKNRIPRYFILMKNFWHAIFNYDIFHFHFNTSFFKNNADLYILKKLNKKIIMQFWG
                     SDIRIYSIGMKINPFLSEIKSNPKKEKKRLEKIKKISKFVDAAIVSDGELFYYIKDYF
                     KKIFFLPQISDISIFRFPSLEKKEPVIIHAPSNREFKGTEKIIEVIKELEKKYKFTFK
                     ILENIPNEQILNEVLNADIVIDQILIGTYGIFSIEAMGCGKPVICYINENYRKFYPEE
                     LPIVSASIDNLYVQLEKLIKDGKLRHEIGKKSREYFEKYHAIDVVTKKLIEIYNEVIN
                     GDIRKK"
     gene            complement(11079..11609)
                     /locus_tag="PLW95_02695"
     CDS             complement(11079..11609)
                     /locus_tag="PLW95_02695"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016886263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOV21573.1"
                     /translation="MKKEQKLYFSHGDGKIKRDKIKKVGKNVIFEKGVLIFHPENIEI
                     GNNVYIGHYTILHSYYKSLMKIGNNVWIGPHSFFHSAGGLIIKDNVGIGPKVIIITSQ
                     HNLSFLDIPIIEAPVEFAPVEIEENSDIGAGSIILPGVKIGKGVVIGAGAVVTKNIPP
                     FEIWAGVPAKKIKERK"
     gene            complement(11844..12848)
                     /locus_tag="PLW95_02700"
     CDS             complement(11844..12848)
                     /locus_tag="PLW95_02700"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOV21574.1"
                     /translation="MRLTNKTSEHIKRKMGSAFIYKIYKRRQLLDKVPYAMNIEITTR
                     CNLKCWMCPRPKHKNVKSGDIDFYLFKKTVDEMTHLLTKKTTFTPVGLGEPLFYNKLL
                     EAIEYLKIKCPNVPIHIDTNATLLDEEKSEMLCRLLCKNDRILFSLNAGSRNTYEWMM
                     GVDKFDLVVSNIKNFLEIREKIGKGPRVIIQIMDTKKTASEIEGFKNFWKYFIGPSDS
                     IYVRPLLNWGGKIETKDICTQVKRKRYPCLSPWIAVVIDINGNVYPCCEALSTRENGN
                     LLLGNIQRNSLLEIYSQDKIRQIRENMLNNQNSISECSNCDFWSFYSNIWFNMKGRWR
                     "
     gene            complement(12845..>13456)
                     /locus_tag="PLW95_02705"
     CDS             complement(12845..>13456)
                     /locus_tag="PLW95_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008420664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOV21575.1"
                     /translation="GSFGDLGCFSFHPRKVITTGEGGIITTNNDELDDKLRKLRNHGM
                     EYYENKNDFVSVGFNSRMTEMQAAIGIVQMYKIDEIISKRQKLAQLYTELLSKVSWIK
                     TPQTINGANHIYQSYVILLKTGINRDRLIDKLRELGIETNIGTYAIHQTKYYRNKYGF
                     NANDFPNARIAFEQSMALPLYPKMTEDDLIHISQKLIEVGNRQ"
     assembly_gap    13457..13466
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13467..13976)
                     /locus_tag="PLW95_02710"
     CDS             complement(<13467..13976)
                     /locus_tag="PLW95_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008420664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HOV21576.1"
                     /translation="MIRLTIPEIDNEEIEAVRKVLNSGWLIQGEKVKQFEKLVADFVG
                     VQHAIAVNSGTSALHLSLLSLGLLEGDEIITSDFTFTATANVIEIVRAKPIFVDIDLN
                     TYNLDVSQIEKKITHHTKVIIPVHLYGQVADMKTILDLAHRYNLKIIEDAAPALGSTY
                     KINDEVRKAG"
     gene            complement(13973..14962)
                     /locus_tag="PLW95_02715"
     CDS             complement(13973..14962)
                     /locus_tag="PLW95_02715"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOV21577.1"
                     /translation="MDIQKEKIRNKIFFITGGAGFIGSSFIKEIIENNEVIVYDNLDR
                     DSLTKREFFHSKNLTLIKGDILNFSKLKKSIPKDINFVIHMAAIAGVDTVIKNPVKTM
                     EINIIGTYNLLKALYEKKLIKKIERFINFSTSEVFGVNAIRVEEKQPSNLQPVGEARW
                     NYAISKLAGEFLTFSYYDKFGLNCTILRPFNIYGPGQIGEGAIHHFVVRAIKNEPLLI
                     HGNGDQIRSWCYIDDMVEGIKLCIIKEKAIGEVFHIGNPRGTITVLSLAEKIVQLANS
                     KSKLIYIEKKYSDVEIRIPDIRKSMQLLHFKPKVDLNEGIKKTIEWYKKQEIL"
     gene            complement(14979..16076)
                     /gene="wecB"
                     /locus_tag="PLW95_02720"
     CDS             complement(14979..16076)
                     /gene="wecB"
                     /locus_tag="PLW95_02720"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOV21578.1"
                     /translation="MKLLSIVGARPQFIKLMPLVREFKKSSFKSSHVHKILHTGQHYD
                     YLMNKLFFDEFGLPQPDYNLEIGSGLHGYQTGEMLKKIESVLLNEKFDIVIVYGDTNS
                     TLAGALASVKLKIPVAHIESGLRSYNKHMPEEINRVLTDHISDFLFCPTSTAVENLRR
                     EGIKRNIFLVGDIMFDSLKISLMIAEKKKIEIIEKLNLKPKEYILATIHRAENTDNKE
                     KLCNIFSYFGEISNKIMPVIVPLHPRTKKMLGLYEKEKDFKKIKIIEPVSYFEMLMLE
                     KKAKIILTDSGGVQKEAFWLGIPCITLRDETEWVETVKTGKNILVGTDKKRILRAVKK
                     FYKLDNTSSSKINKKKNVSLKILKILFSKTK"
     gene            complement(16073..17242)
                     /locus_tag="PLW95_02725"
     CDS             complement(16073..17242)
                     /locus_tag="PLW95_02725"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017302733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOV21579.1"
                     /translation="MDNKKQIPVVDLKLEYDYMKDEINSAIQKCLEHQKWILGPEVND
                     LEEKISNYLSVKYCIGTSSGTEALVLSLRGLAIKLKGREFFNKEDLIITTPFTFTATG
                     DSILRSGATPLFVDVDLNTYNIDVSKIDDVLTKSPYKGKIIGILPVHLYGLASNMDKI
                     MEIAQKYNLFVVEDTAQAFGGSWKGKKLGSIGNCGCLSFFPTKNLGGFGDGGMISTND
                     EEINDIIRCLIKHGGKDKYNVEHIGYNARLDTIQAAILLAKLKYVEEFNKKRREIANI
                     YNENFKDIEEIETPADLEEGYNVYHQYTIKVRHCKRDEVKKYLEQQGISSMIYYPISL
                     HKMKVFEGRCEIYGSLENSEKLCKEVLSLPIEPLFGRSEIEYIVKKVKEFFKYEK"
     gene            complement(17776..18354)
                     /locus_tag="PLW95_02730"
     CDS             complement(17776..18354)
                     /locus_tag="PLW95_02730"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOV21580.1"
                     /translation="MSKVFIHPTSVIDENVEIGEGTKIWHFSHILKDTVIGENCVIGQ
                     NVMIGPDVKVGNGCKIQNNVSIYKGVVLEDYVFCGPSCVFTNVINPRAFIERKNEFMT
                     TIVKKGATIGANATIICGITIGKYALIGAGAVVSKDVSDYALVVGVPAKQIRWVCKCG
                     TTLKFDGKIATCNYCGNKYKLENNILLPVKEK"
     gene            complement(18990..19952)
                     /locus_tag="PLW95_02735"
     CDS             complement(18990..19952)
                     /locus_tag="PLW95_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOV21581.1"
                     /translation="MKIGIIGAGWWGKNLVRVSYELGVLKTVCDIDERTLNDIKVKYN
                     EVKTTKNFDEIFMDKEINGVIISSSAETHYSLVKKAILSGKNVFVEKPLSLKVSDGKE
                     IVELAEKNKIILMVGHILHYHPAVIRMKEMIKYGEIGQIQYIYSNRLNIGRIRNEENI
                     LWSFAPHDISLLSSITGQLPDKVFSFGGTYLPHNVEDVTLTFFSFPSGIKGHIFVSWL
                     NPFKEQKFVVVGNKKMIVFDDIEKERKLVVYSHKINWKENEIPTIEKCEGEVIEIEKK
                     EPLKEEIKHFLNCIEKGEKPLTDGREGLGVLAVLEMAEKSLRSN"
     gene            complement(20165..21175)
                     /locus_tag="PLW95_02740"
     CDS             complement(20165..21175)
                     /locus_tag="PLW95_02740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOV21582.1"
                     /translation="MPQKILIIKTGALGDIILSSVFFQTVKKNFPDADIYIITKKIYN
                     EMVENYPIFKKIICLSNKFILDLIKVYPLKRKKFDIIFDLNGNLRTNFLSFFIGGRKR
                     YGFYRTKLGKKLLTNGINKKDIIEKNPIDRQFLLLNYLNISSENYVKVPKIYLNETDK
                     KKFENFKREVGFNENYNWIAIHPLSSEKWKTKRWGIDRFAELSDILIKNGYKVVIIGD
                     KNGIEYVNQIIKKMKYKPLNLVGKTNFTNLCFLLSNVKILITGDSGPLHIGAALGIRT
                     LGLYGAFLPERHCPNLPNVSYIYKKVACSPCYKRKCDKMLCMKEIKVEDVLEYIFKEN
                     QK"
     gene            complement(21177..21401)
                     /locus_tag="PLW95_02745"
     CDS             complement(21177..21401)
                     /locus_tag="PLW95_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21583.1"
                     /translation="MRKYNFDKFKMENLKKAILLRAEIFGNNSKRRKRNVRDKEEWSV
                     FFILTEARKKKMEKKGELIEKNKRTYKLKI"
     gene            complement(21388..21942)
                     /locus_tag="PLW95_02750"
     CDS             complement(21388..21942)
                     /locus_tag="PLW95_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21584.1"
                     /translation="MRKLPDEFKKLKRIKEPFKRRLYFVAILTKYLKKEKIKPIIVGG
                     NAVEFYTLGSYATGDIDIVISVPEMVINLLLQWNFKKIGRVWVNENLDIELDIVSDIL
                     SGDISKVSEVIIDDLKVYIEGIEDIIIDRLNAALWWKSDEDKKWAIFLLKLYKEKLDK
                     KYLQEQAKKEGLIEFLKEIENEEI"
     gene            22224..22391
                     /locus_tag="PLW95_02755"
     CDS             22224..22391
                     /locus_tag="PLW95_02755"
                     /inference="COORDINATES: protein motif:HMM:NF021560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HOV21585.1"
                     /translation="MWIDYDEEADVLYINFNRPQKATDSEMTNDGILLRYKDEELVGI
                     TILDASKRQTE"
     gene            complement(22683..23735)
                     /locus_tag="PLW95_02760"
     CDS             complement(22683..23735)
                     /locus_tag="PLW95_02760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOV21586.1"
                     /translation="MRVLPSMDFGGIERGVYDFSIKASELGHEIIIVSGYGRFIPQLI
                     KNKNIRWYNLPLEKKNILNFFKGRKKLLKIIKKEIPDIIHVQSRFPSWIICSLKSKLE
                     NIPVVTSIHSIYPFYLYSQSTGKGDLVIVVSKFLEEYAIKKLKVNINKIRLVYNGISD
                     EFSRIEKTKKEKIVIGMIGRFTKSKGWFYFLEAVKDLYYENKYDFETILIGSGSKSYE
                     KKIKKWIEKNNLSKKIKILKCNSIDGLKLIDLLVIPSIKAEGFGRTVVEGQFAKVPVI
                     ASNIGAIPELIEDSKTGFLVSPKDSISIAEKIKYLMNNPEVIEKITDNAYKFVKENFS
                     VDKMVEKTLNVYKELL"
     gene            complement(23741..24760)
                     /locus_tag="PLW95_02765"
     CDS             complement(23741..24760)
                     /locus_tag="PLW95_02765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOV21587.1"
                     /translation="MCPKNIKEYKLRRILIIGLSCLGDNLLLTPSIKKIKDAFPDASF
                     DVIIGPRSVDFAIDNPWFSQYFVYNKKKGLLKLIMQVRKNRYDLIVDFRNSLIPYFLR
                     GKYKLTFFLKEFFSDKIFTHEAERNLSFIEPFFGREKNIQLYFPLNKVYKDKGEEILL
                     KYGIKRSDNIVVLCPGANFEGKRWDKNKFAQLGKELFRYYDIKIIAVGSKNEILLANE
                     VIQKIDNKNAFSLAGKTNIRELAAILEMSSLLITNDTGTMHLASAVGCPIVAIFGPGN
                     PYRYGPIGVKNQVVHSGMNCFPCKVESKCKIDFECLKRINVEHVLKAAMLILDEKQHR
                     FLFEI"
     gene            complement(24727..25806)
                     /locus_tag="PLW95_02770"
     CDS             complement(24727..25806)
                     /locus_tag="PLW95_02770"
                     /inference="COORDINATES: protein motif:HMM:NF018017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELM1/GtrOC1 family putative glycosyltransferase"
                     /protein_id="HOV21588.1"
                     /translation="MKSEFKILLLDDGNKGNFVQSYGIASVFPSTKIEILKIEFKGPR
                     YKLPGRKGNYPVFIKFFNLFCFFRMFKMGNKLLKLYSKNLKEFSRKKYDFIISSGSLL
                     APLNILLSKETSSYSIHLMVPSGMPLSLFDFLIIPYHDFLKFKIQRKNIIVTLGAPNL
                     INEEMKKNELDKIPVSLKEKIKKKLITIVIGGDDQNYRISTNWVKNIKEEIDKMKENF
                     QFFLTTSRRTNEKVIQYICENFMKDKNFLYIEIPTISKETLYPEILFISDIIMVTEDS
                     INMISEAASTGKKVIILGVERKKKKKLIFDLTIEKFVEDGYAEYIPFTEIDKLPDMVK
                     IVLNKKYKVLNEAKECVQKILKSIN"
     gene            complement(25851..26606)
                     /gene="hisF"
                     /locus_tag="PLW95_02775"
     CDS             complement(25851..26606)
                     /gene="hisF"
                     /locus_tag="PLW95_02775"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOV21589.1"
                     /translation="MSSVRIIPCLDIREGKVVKGVHFENIREAGDPVENAKFYCEENA
                     DEIVFLDINATIEGRKTTLDVVKKVAEVVFIPLTVGGGISCVKDIEELLKSGADKISI
                     NTAAVKNPSLVSEAVKKFGSQCIVVAIDAKKVTKDSWEVYIESGKTPTGIDVKEWAKK
                     VEELGAGEILLTSIDRDGTNRGYDIELTRKVSENVKIPVIASGGAGNLQHLVDVVKKG
                     KASAVLLASLLHFRKYSILEIKKYLAQNGIEVR"
     gene            complement(26625..27239)
                     /gene="hisH"
                     /locus_tag="PLW95_02780"
     CDS             complement(26625..27239)
                     /gene="hisH"
                     /locus_tag="PLW95_02780"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOV21590.1"
                     /translation="MVGIINYGAGNIFSIYSAIKICGCTPFLIENSKDIKKAGIIVIP
                     GVGAFDDGMNFLIKTGIGEVIKTEIKRGKFFLGICLGLQLLFQKSEEGNLNGLGIFDG
                     IVKKFSFSDISIKVPHMGWNKVKQEKKNILFSNIPDNSYFYFAHSYYADVNRKEIIYG
                     ITKHHIEFPSIVIENNIVGVQFHPEKSGKYGLQFLKNFFLTSLK"
     gene            complement(27951..29498)
                     /gene="carB"
                     /locus_tag="PLW95_02785"
                     /pseudo
     CDS             complement(27951..29498)
                     /gene="carB"
                     /locus_tag="PLW95_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012940061.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
     gene            complement(29513..30445)
                     /locus_tag="PLW95_02790"
     CDS             complement(29513..30445)
                     /locus_tag="PLW95_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV21591.1"
                     /translation="MNIDKFLKEKIAEIKKKVVKNEKALVATSGGVDSVVAAIIAKRA
                     LGDNLYVLLLDTGFMREGEIEETKNFFEKEGITLEIWDVKEKFFEALKGKIDPEEKRM
                     SFRECFYKTLGQAVKSYSVDFLIQGTIAADIVETQKGIKTQHNVLSQVGINPLSFGLK
                     IIEPLKELYKPDVRKLAKALKFPKKIYDRQPFPGPGLSVRVIGEVTPDRIRKVRIATS
                     IVEKTLTSSKIFQVFPVLLSDKATGIISGKRVYGDIIAIRAVESKDAITAKVPKIQLS
                     KLEKVMKEIVEKVPDVVKVLYDITPKPPSTIEYI"
     gene            complement(31033..31779)
                     /locus_tag="PLW95_02795"
     CDS             complement(31033..31779)
                     /locus_tag="PLW95_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOV21592.1"
                     /translation="MNRKRVRISASAVSCGGGFTLIELLVVIAIIAILAAMLLPALSK
                     AREKARQSVCMSNLKQIGTATMMYCQDYDDWLPCGYVAGGGISGQHDGYASADIPAWY
                     VLLAKYFNLPVYDYYNLGTTANGLYKPCVYTCPSQKFKYPNVRPVSYAFPLDTAFYGI
                     TIRNDPVTKMGKLNRVKDTSAKVWIADSYQANTFNPYLLLPEKTWESSGLRRHNNGGN
                     CLFFDGHVEWVSYERANSVGGVKPMFLPYN"
     gene            complement(32893..33330)
                     /locus_tag="PLW95_02800"
     CDS             complement(32893..33330)
                     /locus_tag="PLW95_02800"
                     /inference="COORDINATES: protein motif:HMM:NF015971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21593.1"
                     /translation="MFYASHNIEKAEFMCLATSKDLFNWEKHPFNPIFLPSKEWAFWD
                     IKKSCSCRDPHVIFEKEYGFMMYYVADIKKDITFSCIAVATSNDLIHWQDLGPVLIRK
                     KSFYEGTCCKTESPCVIKYSNRYYLFYRHGNGTKFSISDNPLD"
     gene            complement(33306..33644)
                     /locus_tag="PLW95_02805"
     CDS             complement(33306..33644)
                     /locus_tag="PLW95_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21594.1"
                     /translation="MEKKFIIPVKKWPKSFKYVYHNKGEYVNDHCIIREKEVFHLFYI
                     SGEVGKGCYDLGNEIIIGHAISKNLYKWQKVEDALVYNPQLPWENRGIYAPYVIKHKK
                     SFICFMQVII"
     gene            complement(33913..36192)
                     /locus_tag="PLW95_02810"
     CDS             complement(33913..36192)
                     /locus_tag="PLW95_02810"
                     /inference="COORDINATES: protein motif:HMM:NF039643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6067 family protein"
                     /protein_id="HOV21595.1"
                     /translation="MKGRFPVREKIILVQILFFLFCFMLFSNETFKEDIGKKLNFSFE
                     LVTENFIPENWTIWKVGKAQGIAEIDENTFYEGKKSIKISNAKGTFGWGYQSPFSIEE
                     IKRIVGPEMEIKISVYVKGENVSGLPYIGLDWWRESIAGWISKSEKHLPTGTYDWQKI
                     EFVVNQIPQNAKYFRIQLATYSCNGTIWFDKLELTVNNKKIEEILAQISEKEKAISSK
                     SALVSENSNVRIWYEIPTQKVFPESIPPINKSEEIIIKCAKGEYEPFQLVLTPYKNFE
                     KVNIDFTNLFYSQNSKVFINKNNFSCYEVGVITYSIKGKIPDPLIPIESFIELKEGES
                     KSIWINVYVPEGVNSGEYKGEIILNFENKLEYKVPLKLIVWDFSLPKKTNLEIVANLW
                     GSPILQVDKRPWDEILKDYIPDIVSHRIRGVSIGLPNAKFQNGNMYISKEDLEVLEKR
                     IKFLKDCGIDIFITSAGPWINTGSTYIGQLKWNSDAIWRPESLEIPIKIMSEDFKKYY
                     KQYHEILSDFFEKIKIRDNVLLYIYDEPSYEALKNLCEICKFLSDINPPLKIFMTSVP
                     NALLNPYISVWCPPTSSIDDNLKLMTMRQKKGEKIYLYHNYLYLINQPALNSRIMPWI
                     LYKYNFDGYLFWSINAWGQNPWEIIKSDGDGYLLYPNLKGEGKPCSTIRWELWREGIE
                     DYDYFVMLEEKINNLNQNKNLGVKERESVHKAEITLCKIYTLVKSWNNYSKDPVDYYN
                     LREEIANYIIELNKIEKLF"
     gene            complement(36266..36928)
                     /locus_tag="PLW95_02815"
     CDS             complement(36266..36928)
                     /locus_tag="PLW95_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOV21596.1"
                     /translation="MRRKYLEFPSLVKRGLGRFSKVSGFTLIELLVVIAIIAILAAML
                     LPALSKAREKARQSSCMNNLKQYGIAFYLYAQDFNGYLPPDTFPLSYGGDLTSWCRNS
                     FFLSYIYGTNRKKGADYPKPYIWICPSDRVRTNGYSYGMNQVARLKKLDRCRRPSSTF
                     LAMDFYSEFWNPWWNIGLENPVWRHNDGINVLFVDGHVGWEHKRTLGGRVLGSDPRFN
                     GW"
     gene            complement(37419..37559)
                     /locus_tag="PLW95_02820"
     CDS             complement(37419..37559)
                     /locus_tag="PLW95_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21597.1"
                     /translation="MRGKIIGLRDKVDSITNIEKVKERMERIESRDNIEEQFTEKKKG
                     KI"
BASE COUNT        12289 a         6565 c         4526 g        14246 t
ORIGIN      
        1 atttattttt atattttgtt tattttaaca ctaaattttt taattgtaaa tacaattaca
       61 aaattgtctt agcaattgta ataacaatta cattttttgc tcctgctttt tttaatatct
      121 ttttcaattc atttgtcgtt gctcctgttg taaaaacatc atcaacaagt aaaatttttt
      181 tatcctttat tgaagatgtt tttcttactt taaatgtatt taaaacattt ttttctcttt
      241 ctgaaccact tagttctacc tgtgatggag tattttttgt cttaacaacc ccatctatca
      301 atacatcttt gccaaataat tttcctaaat aaacagaaat aattgctgat tggttaaaag
      361 ttcttgaaaa ctcttttttc caatgaagag gaacaggaat aattaaatca aattcaggta
      421 ttttttcttt taaattatca tatacaaaaa gggcaagttt ctttccataa aatttctttt
      481 taccatactt aaatttatgt attgcatttt ttataggacc ttcataagca gcaacatgat
      541 aaaataaagt gtctgagttt tgataaaaaa taatttcttt ctcacaattt tggcaaaatc
      601 catacttact caacttttcc tttttgtcgc atccataaca tttttggtcc tgaaataaat
      661 ttaaaaatag acataaggca ttgtttgtct tacaataaat tgtatgaaaa aacaacttaa
      721 acatatttat atctccaaag agttttttat aaattttatc atatcatttt taattttcac
      781 ctttattttt ctcataaatg gtatttttaa agcaatagaa attattatta aaggtgcttc
      841 ctttttttca gtagttaaag ttcttattta ttttttcctt tcttcattac catatatttt
      901 tcctttaacc cttttaagtt cttcaacatc tgtcttttca agattaactc atgacagaga
      961 aatccagata ttttcatttt ctggaataaa tccccttatt cttacaaaaa atcttttaat
     1021 ttttgctgtt ttatgttcta ttttacttat ttattacaat ttacttatat ttccatctat
     1081 taaatatcaa agtagaaaaa caatttataa tttaactgca aaaaatccta ttgccttttt
     1141 tcaaccaaaa acaattataa atgattttcc tgatttttct atatatattg gtgatttatc
     1201 aaaggactta aactttaaaa acctttctat tatacagaaa agtgacaaaa taacactttt
     1261 tataaaagca gaagaaggta aaataaatta tgtaccagaa acaaataaca taattttttt
     1321 aatgaaaaat ggatttataa catctcaggc atcaaataaa atctcaaaat tggacttttc
     1381 cgaatatacc tttcatattg aacttccaga aagtttttca tttaaagaac cacaaaaaaa
     1441 attatcagaa ttgcctctat ctcaattaaa aattaaaaat aatattgatg caaatattga
     1501 aataaatgaa agaatctctt ttggaattac acctttaatt tttgtttttc ttggaattgg
     1561 tataggaatg aaattaaaaa ataaaagcag ggtcttatgt attggagtag caggagcaac
     1621 aagtttattt ttctttttat ttttgatttt aggagaaatt attgctaaaa aattaaattt
     1681 tagtttgttt gtctatttac ctgttcttat tttcaccttt ataacatatt atttaaataa
     1741 gttttcagtt agataatttg aacagtttgg cagaattggt acttcccact ttgaaaaagt
     1801 taagataaaa gaacctggtt ctcacagaga accgggttct tgaaaaaata agataacggg
     1861 gaattttaaa aattgtcgct tttgtgcttt aaaaatatta tttgataaaa tggtaaaaat
     1921 aaaatatatt ctttttaatt tttttaagtt atttttaatt tcttctttat gccttctttc
     1981 tttatttttt ataatccaga ttcttgagga cttaccagat ataatttcag gaagccaaaa
     2041 atttttatta tcaaattata ttcttacact tccttcttca tttgttctca tttctcctct
     2101 tataaccctt ctttctggga tgttttttgt ttccgagatg gtaaaggcaa atgaaataaa
     2161 aatatttgaa atatcaggga taaattcatt aaaaattttc tctatgtttc tttttgcctc
     2221 attaataata agttcattta cattttatat aaaaaattat gttgtcccaa aaaccataaa
     2281 aggaaatgaa tatcaaacat caatttcttt ttcatcccct tcatttcttt tcaaatcaga
     2341 caggtatgag aaagatggaa aatttataaa tatagagatt tcaataattt tagaagatgg
     2401 tagtataact tcaataagag caaaagaagg aattgagaag gagaaaaatc tctggacatt
     2461 taaagaagga aaattctact taattgataa aaatacaaat ttaaaaaaag aagaaaattt
     2521 taattcaaaa acatttgaat taccaataac atctgaaatt ttgttagttg ctggtaaaaa
     2581 cccggattca atttttataa atcaactaaa aagcacaata aaggaattga aaaaaatggg
     2641 attttcgcca tttttattag aaacatattt taatgagaaa attgcatatc ccttacttaa
     2701 tctttttatt ctttttgcta ttttcccatt ttttcttagt aaacagaaaa ttacgagatt
     2761 tcttgtttta agtttaacaa ttgttattgg atttttaagt tatgggattt attcatttct
     2821 tatagcacta tcaaaatcag gtaaaatccc accttttttt ggttgttggg gactacatct
     2881 ttttgttttt atatcttttc tctttaccct attcaaattc agatagggat taaaataatt
     2941 aactacttta cttatatttt ttaaaaacta cctttattcc ttctctccac ttttataaac
     3001 caactgaaat agcagatatt aacaaaactg atttgagagt tcttcaaaac catttgttgt
     3061 attgaggatt ctaattcttt tctataaatt gatttatgta atttatgaaa ttgtagaaat
     3121 catctaagtg cttatgcaaa atctcataaa ttttggtcag gtctatatca acatattcat
     3181 gaactaaaat atttcttaat ccagccattc cacgaatttt tttactaaat tctattggaa
     3241 ttatattctt ttctcctaat ttgtctataa tgtctacata gtcttaaatt tggttttcat
     3301 tcagtgtagc taaaatatga ttgcctacat caatgagcac ctgaatagaa agttgtaaac
     3361 catgacagat tatccattgt ttgcttaaag atgaagaaaa ctcttcaaaa gatatgcttt
     3421 tggatttttc taattctttt agataccgct ttaactcttt aaatttctct ttaattattt
     3481 ctttgtcaat cacttattct cctgttgttt tataaactgc ctaaaatcag catatttatt
     3541 gatagtatta acttgaaatt ggatacgatt cttttcgttt ttagagtaaa taaccttgcc
     3601 aaaatataaa actctatgtt taagtaaagt attcgcatca ttaagaataa ctaaatctat
     3661 attatttgtt ttcagtaatt taattaaatc tgttaatatc tctgctttat atccatatct
     3721 atatgattgc tcattaattt gtcctttgtc cactattata cctatatcaa tatcgctaag
     3781 aacattatct ttttcttgtg ctactgaacc aaaaacataa gcaacttcta tttccgggtg
     3841 tttttgaaaa tactctttta gaattgtctt aatttcattt acactttttc tcattttcta
     3901 atatcaactt gtcgttttat atacagaaat aacttattcc tcttcaataa ctttttctgc
     3961 ataaagaaat gctgccctta tatcttcttt ttttagacag gggtattctt taagaatatc
     4021 cttctctttc cagttttgag cgaccaattt aacaattaat tcaacaggaa tacgtgtcat
     4081 atggataatt ggtttgccta ataaaatttt tggatttatt tctattctat tcataaaatt
     4141 ttccattatt tccctctata ttaaaattag gttctgccaa atttggctaa gtattttaag
     4201 tggtttttcc ctggttttag agccgttttt acggtactct gggttttctt aaatccccct
     4261 ctccttaatc tcccctgccc ccacctacaa acaacggcgg gacttacagc ccgagaataa
     4321 gaaatggacc ccttcccttt ccttttgccc cttcctttga caaaggaagg tggggatgga
     4381 tttgtattaa atccccctca atcccccttt ttcaaagggg gatgtagtaa ttctcccctt
     4441 ggtaaagggg agttagtggg aatgtttatt ttctatctct ttcttgacac tacttaaaat
     4501 tatacagcaa aaataaaata ttatcaagga ttttgaacta tatcccggta tcttattgct
     4561 aattcccatt tatattttcg ccacagagac aataagcaca atttttcccc gttgtgctta
     4621 gtccaacaaa attacatcca tacctctgtg taatacccta taaattggtc tctttcaaaa
     4681 aaactgcctt tattccttct ctccatggga atctgaaaaa actcccttta taaataaatc
     4741 cacttttttc acatatctgt tttaatgttt ttagagaaaa ataaaaaaga tgttctggta
     4801 tgctcaatta attatttaga aatattttgg gattttttac aaaatataaa attttgatat
     4861 aatttaaaat tattataaat ttaaattatt cttatgataa aaataaatat tattctcaat
     4921 atagggaaaa aaccaagtgg agcaggaagg ccaataattg aattttctaa tcagttatat
     4981 aaaaacaact ctgttgtaat ttataaacca tttaacccag aatataaaaa aggggtagag
     5041 tataagttta gagaaataat tggatatatt ttaaaaggga gagagtataa acaaaaatgg
     5101 attgagtgca aaccaaaggt ttttattatt cccaagtgca aagaaaaatt tattagggat
     5161 gctgacattt tattttttcg ttcaccaaaa tttttagaag aaataggaca tttttcttta
     5221 tctaagggaa aaaaagtaat gagggtttct gacatatttt ttgctttaaa tccaccaaag
     5281 aatattcctt ccgatattta tataattaca tcttcaacaa tggttaaatc attactccag
     5341 cagaaatttc ctgataaaag aatttttctt gtagttaatg gggttaatct taattttttt
     5401 ggttttccag aaagaaaacc aatttttaat ggaagaatag ggatggttta ttattcagga
     5461 aaaaaccccg aacataaagg aatggaagat ggtttctggg ttatgaacat gttaaaagag
     5521 aaatatcctt cattagaatt ttatctattg ggctgggaaa aagagaaaaa aattccccca
     5581 tttgtaaaat ttacagaagg attaacttca gaaaaagtaa gagaatttta tagacaaatt
     5641 gatattctta tttacccaag taaattagat gcatgcccaa atccacctat ggaaggtatg
     5701 gcaagtaaat gtgcagtaat taccacaaat gtaggaggaa ttgcagattt tgttattcct
     5761 ggcgtaacag cccttgtgaa tgaaccaggg gaaaaagcag aaatgcttaa aaacgctatt
     5821 gccttaattg aaaataaaaa actctgggaa aaaatttcaa atgctggata tgaaaaaata
     5881 aaagaattta gttatgaaaa acaagggcag aaattggaaa atgtattttt acagatttta
     5941 aatgacaaaa attaacttta taaagagaaa aaatgaatat aggaatgatt ttagaagatg
     6001 agtttccacc tgatattaga gttgaaaaag aagcacaagt tttattatct caacatttta
     6061 atctccattt actttcagca gtaaaaaaac aggaagaaaa aagaaaagaa tataaaggaa
     6121 tattagttga acaaaagcct attatatatc ctaatgtata tcatcacctt ttggataaat
     6181 taaattttct tagagggaaa gagacgttat ttcataaaca aatagaacaa tttgtaaaaa
     6241 gaaacaaaat tgaaataata catgctcatg atttgccagt tgcttttgtt tcactaaaga
     6301 tagccaaaaa acttggtatt cctataatcg ctgatctaca tgaaaattat ccatattata
     6361 aattgataaa aaaaagaaaa aatacaaaaa gattcagata ttggttgaat atagaaaaaa
     6421 aagttgtaaa ggaatctaat tttattataa ccacctgtga agaaatgaga gaaagaattt
     6481 tatcagaaca ttgcatacca tcagaaaaaa tttatgttgt tcctaattat tatggaggag
     6541 aatttgaaaa tattgttgtt gataaaagta tattagaaaa atactcacaa aaatttatga
     6601 ttctatatag tggagttgta tctttacata aagggataga gacattgata aaatcaatgg
     6661 agtatcttaa aaattaccct gaaattgtat tatgcattgt tggagttaaa aagaacaaag
     6721 aggaattcga aaaattagca aaaacgttgc atgttgagga aaaaattgaa tttataaaat
     6781 ggcaaccaat agaaaaaacc tactcttttt atttagccag ttctgtttgt tctgtacctt
     6841 ttgaaaaaaa tgtacaaact gattgttcat ctcctcacaa actatttcag tatatgcaat
     6901 gtaaaaagcc tgttttagtt agtaattgta attctcttat aagaatagtt gaaaaatcca
     6961 attgtggctt tgtctttgaa tgcgggcatc caaaagattt agcagataaa attttaattt
     7021 tatataaaaa taaaaagttg agaattgaaa tgggagaaaa tagtgcgaga tctgttaaaa
     7081 ataactataa ttttgaaata gcaggtagcc aattatcact tctttataat aatttaacaa
     7141 aatggttcaa aaaatgaaaa aagtcctgat tattacatat cattttccac catcaaaaaa
     7201 agtaggtgta ttaagaataa agggattagc aaagtactta cctgaatttg gttgggaacc
     7261 agtttttgtt attcctcttt tacaacagga accagaaatt aattctcaaa ttatagaaac
     7321 tcatccgcca tatcaaaagt tttttaagta ttatttttca aaggcaaaaa aaagaataaa
     7381 aaaaatttct ttacctcttc ccccatttca taaaagagtg aaaagttttt tccgcgggat
     7441 gattgatatt cctgataata gaattggatg gttgccattt gcctatgagg caggaaaaaa
     7501 tttaatagaa aaagaaaaaa taaatcatat gataagtatt cttggacctg ctacctgtca
     7561 tttagtaggt agtaatttaa aaaagagata taaaaatatc ttctggatag cagatttcag
     7621 agacccctgg acactttaca aaaataatac cctgataaat aaatataaaa gaaatcttga
     7681 aatagaaact ttaaaggaag cagatgcaat tacaattgtt acaagaaaat tggcttatga
     7741 tatgaaaaaa attcacaaaa ataaagatat ctattttatc cctaatggct ttgaccctga
     7801 agaaattgat ttgcaaccac aaaaattaac tgaaaatttt accataacac atactggttc
     7861 cctttttata aataaaagga gcccagaact attatttaaa gcactttccg aactaataaa
     7921 agaacaaaaa ataaaggcaa atgatatttc tataagattt tttggtaaaa atcaaaattg
     7981 ggtattgcct ctggcagaaa aatatggatt aaaagatgtt gttaagtatt atggagaaag
     8041 agaaagaaaa gagtgtttaa aaaaacaaag agaaagtcaa cttttactta ttatacaggt
     8101 agaaggaaaa ggagaagaag gatgtttaac tggcaaaatt tttgagtatc ttacaagttt
     8161 tagaccaatt ctttcaattg gagacaaaga taatgaagta tctaatctcc ttaaagaaac
     8221 agattcaggt attcactgca caacagtttg tgaactgaaa gaaacagtac taaaatatta
     8281 tagggaatgg gaagaaaaag gatatattgg ttactcaggg ataaaagaag aaattatgaa
     8341 atatagtcat agagaaatgg ctaaaaaatt tgctgatatt ttaaataaaa ataattagat
     8401 gaataatata aaagatattc gccatagaat tgaaagattt tttaaacaat tagttgggct
     8461 ggaagtatgg agtaaaatag aagttttttg cccatatgaa atgataggta attattgtgt
     8521 atccttttta gataaaatta atagtgattc aataatttat tcacttggag ttggtagaga
     8581 tatatccttt gatttagatt taataaataa aaagggagtt attgttcatg cctttgaccc
     8641 aactcctata gcaaaaaatt ggatacaatc aaaaaatctt ccaaaaaatt tcaaatttta
     8701 tccttatgga atagctaatt ttaatggatc cgctgaattt catattccct ctaatcccaa
     8761 aagcacttca tactctcttt tagattctgg gaaaacattg ggtacagtaa aatatcctgt
     8821 gaaaagatta aaagaggtaa tgaaagaatt aggacatact aaaatagata ttttgaaaat
     8881 agatatagaa ggtggagaat atatggttat tgaagacata ataaattcag agattaaggt
     8941 tattcaatta cttgtagaat tccaccatag atacaaaaaa attggacttt ataaaactaa
     9001 acaaacaata aaacaattaa gagaaaacgg atacaaaatt ttttatattt ctcctgatgg
     9061 agaagaatat tcttttatta aatataatga aattttcaat aaataaaaaa gcaacactat
     9121 gtttaacatt aaacgatttt catcctaatc aggatgatat tgataggttt attaagaaaa
     9181 ataaaaaact ctcatttgag ggattaaaat tttttcctat aagtaaattt tattttcaat
     9241 cacataaaag aaaaagttat caatattatt ttatttttaa aaaaataaac aaaatacgaa
     9301 tggcatatgc acaatggaaa gggacagaaa ttccaaaagg aagttttaaa tatctaataa
     9361 gagttgatga ttttcctcac tgggaaaaaa attataagga atttttaaag tttgctgagt
     9421 tattcagtaa atataatata ccatttttat tgggagtaac tccttttctc tcattaaatc
     9481 caaagtcaat ttacaatact aaatttaagt ttcttgatga taatgaagta aaaattttaa
     9541 aagaattttc aaatatagaa atatctttgc atggattaac ccatcagtca gtcaataaaa
     9601 aaagaacaac tgaatttatt ggattaactg atggagaagt agaagaaaaa ttaacattag
     9661 gaataaaact attagaagaa aaacaaatat ttcctattgc ttttattcct ccttttgata
     9721 aaattgacaa aaataatttt gagataatta aaaaacactt caaaattatc tgtggtggat
     9781 ttgacactgc taaatatgtg ggatatttaa ttaccccatg tattgttgac aataaaactg
     9841 catttgttcc ctcttatttt ccattatcag gtaatataaa cgaaatacat aatttccttt
     9901 tagatataga agaagaaaat atcattgttc caataactat ccattggaca tatgaggtaa
     9961 ataatcttga aagagttgaa aatttcttaa aatttataga gaaaaaagtt atttcttgga
    10021 aggatttttt aagtactact gaaaacctgt aatttcttct tatttttttc ttatatcccc
    10081 attaataact tcattatata tttcaattag tttttttgta acaacatcta ttgcatgata
    10141 tttttcaaaa tactctctac tttttttacc aatttcatgt cttaacttac catcttttat
    10201 caatttctct aattggacat aaagattatc aatagaagca gaaactatag gcaattcctc
    10261 aggataaaac tttctgtaat tctcatttat ataacaaatt actggtttac cacatcccat
    10321 tgcctctata gaaaaaattc cataagtccc aattaaaatt tggtcaatta ctatatcagc
    10381 atttaaaacc tcatttaata tttgttcatt cggaatattt tcaagaattt tgaatgtaaa
    10441 tttatatttt ttttccagct ctttaattac ttcaataatt ttttctgttc ctttaaattc
    10501 cctatttgaa ggggcatgaa taataactgg ctcttttttt tctaaagaag gaaatctaaa
    10561 aatagaaata tcagaaattt gtggaagaaa aaagattttt ttaaaataat cttttatata
    10621 ataaaaaagt tctccatcac taactatagc agcatcaaca aatttactga ttttttttat
    10681 tttttccaat ctttttttct cttttttagg atttgactta atttctgaca aaaatggatt
    10741 tattttcatg ccaatagaat aaatcctaat atcacttccc caaaactgca taattatttt
    10801 tttatttaat tttttcaaaa tatataagtc agcgttgttt ttgaaaaatg atgtattaaa
    10861 atggaagtga aaaatgtcat aattaaaaat agcatgccaa aaattcttca ttaaaatgaa
    10921 atatctcggg attctatttt tgccatcaaa aattcccttc tgttcgtaaa atttcttctt
    10981 tttaaaaaat aaataatcag catttattct ttgttgcctc aaacctttta caaataagta
    11041 tggttgattt gaaatattag agggcagatg aagtatattc attttctttc ctttattttt
    11101 tttgccggaa ctcctgccca aatctcaaaa ggagggatat tttttgttac aactgcacct
    11161 gctcctataa ctactccttt tccaattttc acacctggta aaattataga gccagcgcca
    11221 atatcagaat tttcttctat ttccacagga gcaaattcaa caggtgcttc aattatagga
    11281 atatcaagaa aagatagatt atgctgggaa gttatgataa taacttttgg accaattcca
    11341 acattatctt taattatcaa accaccagca ctatgaaaaa aagagtgggg gccaatccat
    11401 acattatttc ctattttcat taaactttta taataactat gtaaaattgt ataatgtcct
    11461 atgtaaacat tatttccaat ttctatattc tcaggatgga aaattaatac tcctttttca
    11521 aaaattacat ttttcccaac tttttttatt ttatctcttt taattttacc gtcaccatgt
    11581 gagaaataaa gtttttgttc ctttttcatt gtaatatttc tttcaatttt tcagttattt
    11641 tttttacatc tctttcagtt atatctttat taaaaggtaa agcaactaat tttttagata
    11701 attcatagct tacagcaaaa tcataaggac taacactaca tttatcttta acttgttttg
    11761 tggaataatc tttaactatt tactttctaa attctgatat cgctatgatt tatacgaatt
    11821 ttgtaatcat taaagatgaa tagtcatctc caccttcctt tcatgttaaa ccaaatattt
    11881 gaataaaaac tccaaaaatc gcaatttgaa cattcagaaa tactattttg gttatttaac
    11941 atattctccc gaatttgcct tattttgtct tgtgaataaa tttccagcag agaatttctt
    12001 tggatatttc ctaataataa attaccattt tctcttgttg aaagagcctc acagcaggga
    12061 tagacatttc cattaatatc tatgacaaca gctatccatg ggctgaggca gggatatctt
    12121 ttccttttca cttgggtaca aatatctttt gtttctattt taccccccca gtttagaagc
    12181 ggtctaacat aaatactgtc acttggacca ataaaatatt tccagaaatt tttaaatcct
    12241 tcaatttcgg aagcagtctt ttttgtatcc attatttgga taatcaccct aggtcccttt
    12301 cctatttttt cccgaatttc caaaaagttt tttatgttac ttaccaccaa gtcaaactta
    12361 tctactccca tcatccactc ataagtgtta cgactacctg cgtttaggct aaatagaatc
    12421 ctatcatttt tacagagtaa tctgcaaagc atttcagatt tttcttcatc taaaagtgtt
    12481 gcatttgtat ctatatgaat tggaacattt ggacatttta tctttagata ttctattgcc
    12541 tcgagtaatt tattataaaa caatggctct ccaagaccta caggcgtgaa agtagttttt
    12601 ttcgttagca agtgtgtcat ttcatcaacc gttttcttaa aaagataaaa gtctatatct
    12661 ccagatttaa catttttgtg ttttggacga ggacacatcc aacatttcag attacacctt
    12721 gttgttattt caatattcat tgcgtatggg accttatcaa gtaactgtct ccttttgtat
    12781 atcttatata taaacgccga gcccattttt cgtttaatat gctctgatgt cttatttgtt
    12841 aatctcattg cctatttcct acctctataa gtttttgact tatatgtatc aagtcatctt
    12901 ctgtcatttt cggatacaaa ggtaatgcca tgctttgctc aaaagcaatt cgtgcattag
    12961 gaaaatcatt agcattaaaa ccatacttgt ttctatagta tttggtttga tgtatagcat
    13021 aagtaccaat attagtttct attcctaact ctcttaattt gtctattaac ctatctctat
    13081 taataccagt tttgagaagg attacataag actggtaaat atgatttgca ccattaattg
    13141 tctgtggagt tttaatccat gatacttttg ataacagttc ggtgtaaagt tgtgctaatt
    13201 tttgccgttt agatataatt tcatctatct tatacatctg aacaattcca atcgccgcct
    13261 gcatttcagt cattctgcta ttaaaaccga ctgaaacaaa atcgtttttg ttttcgtaat
    13321 attccattcc atgattcctt agttttcgta acttgtcatc taattcatca ttattggtag
    13381 taattatacc cccttcacct gtagtgatta ccttgcgagg atgaaaactg aagcacccaa
    13441 ggtctccaaa agacccnnnn nnnnnnccct gcttttctca cttcgtcatt tatcttatat
    13501 gtcgagccca aagcaggagc agcatcttct ataattttta aattatacct gtgagctaaa
    13561 tcaaggatcg ttttcatatc tgctacctgt ccataaaggt gtactggtat aataactttt
    13621 gtatgatgag taattttctt ttctatttga gatacatcaa ggttataagt attcaaatca
    13681 atatctacaa atattggttt tgctctaact atctctatca cattagccgt agccgtaaag
    13741 gtgaaatcag aggtaattat ctcatcaccc tccaacagac ctaatgataa gagtgacaaa
    13801 tgtagagcag atgttcctga attaactgct atagcatgct ggacgcccac aaaatctgct
    13861 actaattttt caaattgttt taccttttcg ccctgaatca accatcctga gtttaatacc
    13921 tttctaacag cttcaatttc ctcgttatca atttcaggta ttgtaagcct aatcataaaa
    13981 tctcctgttt tttataccat tcaatagttt ttttaattcc ttcatttaaa tcaacttttg
    14041 gtttaaagtg taaaagttgc attgattttc taatatctgg aatccttatt tcaacatctg
    14101 aatatttttt ttctatatat attaactttg attttgaatt ggctaattgc actatttttt
    14161 cagctaagga caaaactgta attgtacctc ttgggtttcc aatatggaaa acctccccta
    14221 tcgctttttc tttaattatg cataatttaa taccttcaac catatcatca atataacacc
    14281 atgaccttat ttgatctcca tttccatgaa ttaataatgg ctcatttttt attgccctta
    14341 caacaaaatg atgaattgct ccttccccaa tttgtcctgg accataaata ttaaaaggtc
    14401 ttaaaatagt acaatttaat ccaaatttat cataataact aaatgttaaa aattcacctg
    14461 ctaatttact tatagcataa ttccatcttg cttctccaac tggttgtaaa ttagaaggtt
    14521 gtttttcttc tactctaatt gcatttacac caaaaacttc acttgtagaa aagttaataa
    14581 atctttcaat tttttttata agtttttttt cataaagtgc ttttaacaaa ttataagtgc
    14641 ctattatatt tatttccatt gttttcactg ggttttttat tactgtatct acaccagcaa
    14701 ttgcagccat atgtataaca aaattaatgt ccttgggtat tgatttttta agtttagaaa
    14761 agttaagaat atcacctttt attaaagtaa gatttttaga atggaaaaac tctctttttg
    14821 ttaatgaatc cctatctaaa ttgtcataaa caataacttc attattttca ataatttctt
    14881 ttataaaact ggaaccaata aatcctgctc caccagttat aaaaaatatt ttatttctaa
    14941 ttttttcttt ttgtatgtcc atttggatgt attttaactt attttgtttt tgaaaacaaa
    15001 atttttagaa tttttaaaga gacatttttc tttttgttta ttttacttga agatgtatta
    15061 tccaatttat aaaacttctt tactgccctt aaaattctct ttttatctgt tccaacaagt
    15121 atatttttac ctgttttcac tgtttcaacc cattccgttt catctcttaa tgttatacag
    15181 ggtattccta accaaaatgc ttctttttgt actccgcctg aatctgtcag tataattttt
    15241 gctttttttt caagcattaa catttcaaag taagagacgg gctctattat ttttattttt
    15301 ttaaaatctt tttctttttc atataaccct aacatctttt ttgttcttgg atgaagtgga
    15361 actataactg gcattatttt atttgatatt tctccaaaat atgaaaagat attacataat
    15421 ttttctttat tatctgtatt ttctgctcta tgaatagtag caagtatata ttcttttggt
    15481 tttaaattta acttttctat tatttctatt tttttctttt ctgctatcat aagagatatt
    15541 tttaatgaat caaacataat atcgcctaca agaaatatat tcctcttaat tccttctctt
    15601 cttaaatttt ctactgcagt tgaagttggg caaaaaagaa aatcagatat atggtctgtt
    15661 aaaaccctgt ttatttcttc tggcatatgt ttgttataac ttcttaatcc agattcaata
    15721 tgtgccacag gaatttttaa ttttacagat gctaaagcac ctgccaatgt tgaatttgtg
    15781 tctccataaa caataacaat atcaaatttt tcatttaata gcacgctttc tattttcttt
    15841 aacatttctc ctgtttgata accatgtagc ccagaaccaa tttctaaatt ataatctggt
    15901 tgtggtagac caaattcatc aaaaaaaagt ttattcataa gatagtcata gtgttgtcct
    15961 gtatgaagaa ttttatgtac atgagaggac ttaaaagatg attttttaaa ttctctaact
    16021 aatggcataa gttttataaa ttgtggtctt gctcctacta tgcttaataa tttcattttt
    16081 cgtatttaaa aaattctttg acttttttta caatatattc aatttcgctt cttccaaata
    16141 atggttctat tggtaaactt aatacttctt tacataattt ttctgaattt tcaagagaac
    16201 cataaatttc gcatctcccc tcaaatactt tcattttatg caaagaaata ggataataaa
    16261 tcattgaact tattccctgt tgttctaaat atttttttac ttcatctctc ttgcaatgtc
    16321 ttacttttat tgtgtactga tgataaacat tatatccctc ttctaaatct gctggtgttt
    16381 ctatttcttc aatatcttta aaattctcat tatagatgtt tgcaatttct cttctttttt
    16441 tgttaaattc ttctacatac tttagttttg ccaataatat tgctgcctga attgtatcaa
    16501 gacgagcatt ataaccaata tgttcaacat tatacttgtc ttttcctcca tgttttataa
    16561 gacatcttat aatatcattt atttcttcat catttgtgct aatcattcca ccatctccaa
    16621 agccacctaa attcttcgtt gggaaaaaac ttaaacaacc acaatttcca atagaaccca
    16681 attttttgcc tttccaactc cccccaaatg cttgtgcagt atcttctact acaaaaagat
    16741 tatatttttg tgcaatttcc attatcttat ccatatttga tgccaatcca tataaatgga
    16801 caggcaaaat tcctattatt ttccctttat atggtgattt agttaagaca tcatcaattt
    16861 ttgagacatc aatattgtat gtatttaaat caacatcaac aaataaagga gttgctccac
    16921 ttcttaaaat tgaatcgcct gtggctgtaa atgtaaaagg ggttgtaatt attaagtcct
    16981 ctttgttaaa aaattcccta ccttttaatt ttattgcaag acctcttaat gaaagaacca
    17041 atgcttctgt cccagaagat gttcctatac aatatttgac tgaaagataa ttagaaattt
    17101 tctcttctaa gtcatttact tctggcccta aaatccattt ctggtgttct aaacattttt
    17161 gtatagctga atttatttca tccttcatat agtcatattc taattttaaa tcaacgactg
    17221 gtatttgctt tttgttatcc attatatttt ctccttataa gttgtgcata ttgaaatctt
    17281 gctctcatcc cttttaccta tcccttaccc acatcttcta aaatcctctt gggtaaagag
    17341 attcactttg ctctattttc ttttataaaa tagaaaaata ctcccttcct tattgtttca
    17401 agaaccagat tctcacatcc aaaggatccc tctgggcgta gcccaacggg cgagccttcg
    17461 gagagaacca tgttcttcta tcttagccgg aaaaaatccc ccttcaaatc ccctctaact
    17521 cccgttgtgc ttggaaaaga catcacgcac caactctgct ttgctattcc tcgctacctt
    17581 agtcggtttt acaaagggga aaatatcaaa cccccctcat ccgagccttc tcccctttgg
    17641 ggagaaggaa atatattccc ctctcttctg ccccctcatc tcaatcctct cccctgcggg
    17701 gagagggtta gggtgagggg gaatttttta ctctttcctt gtgcctttct gcttttcatt
    17761 tatacttact attttttact tctcctttac aggtaataat atattatttt ctaatttata
    17821 tttgtttcca caataattac aggtagcaat ttttccatca aattttaatg ttgttccaca
    17881 tttacaaacc catctaattt gttttgcagg gactccaaca actaatgcat agtcagaaac
    17941 atcttttgat acaactgccc ctgctccgat aagtgcatat ttacctatgg ttatcccaca
    18001 gattattgtt gcatttgctc caattgttgc tcctttcttt acaattgtag tcataaattc
    18061 atttttcctt tcaataaatg ctcttggatt tataacattt gtaaaaacac aggagggacc
    18121 acaaaaaaca tagtcctcca aaactacccc tttatagata gagacattat tttgaatttt
    18181 acatccattg cctactttga catcaggtcc tatcatcaca ttttgaccta ttacacaatt
    18241 ttcaccaatt actgtatctt ttaaaatatg agaaaaatgc catatttttg ttccttctcc
    18301 aatctcaaca ttttcatcaa taacagaagt cggatgaata aaaactttac tcatttctcc
    18361 tccaaattta aaaaattttt atatttattg ttatttatag taatttctat ttgttaatct
    18421 ccatttatac ttttaggtac taaattcaaa aatccctcaa aatcccccct tccccccctt
    18481 ttcaaaaggg ggaataacaa taaatccctc cttttataaa gagagaaatt aaatccccct
    18541 accccgttgt gctatcctgg aaatccaccc caaccctcct ttaagaaagg agggagttat
    18601 aaaaggtagg aaatatcaac cccccctcat ccccgccttc tcccctccgc cacaccgcac
    18661 ggcggacaga gactggggag aaggaataag aaaggaaata aaatccctgt tttcaaggac
    18721 ccggttctta cagagaacca ggtcctttta tcttattctt tcttaaaatc ccctctatcc
    18781 ccgttgtgct tgctataaat ctcccctcgc ccctgttgtg ctatctaaga aacgatgacg
    18841 caccaactct gaggcacaag gtaagcaact tcagtcggtt tataaaagag gggagtattt
    18901 ttggatgacg caccaactct gccctgccaa tcctcgctac tttagtcggt ttgttaaagg
    18961 gggaagtatt aaggagagaa attaaaatat caattactcc ttaatgattt ctccgccatt
    19021 tcaagaactg ccagaactcc taaaccctct ctcccatctg tcaatggttt ttcacctttt
    19081 tcaatacaat ttaaaaaatg ttttatttct tcttttaatg gctctttttt ttctatctct
    19141 ataacttcac cttcacattt ttctattgtt ggaatttcgt tttccttcca gttaatttta
    19201 tgagaataaa caacaagttt tctttctttc tcaatatcgt caaaaacaat catttttttg
    19261 tttccgacaa caacaaattt ttgttcttta aaaggattaa gccatgaaac aaaaatatga
    19321 ccttttattc cagaaggaaa agaaaaaaaa gttaaagtaa catcttcaac attatgaggt
    19381 aagtaagtcc ctccaaaaga aaaaacctta tcgggcagtt gtcctgttat tgatgaaaga
    19441 agagaaatat cgtgaggagc aaaactccat aaaatatttt cttcatttct tattcttcct
    19501 atattcagtc ggtttgaata aatatattgg atttgcccaa tttctccata ttttatcatt
    19561 tctttcatcc ttattactgc tggatgataa tgtaaaatat gtcctaccat caaaataatt
    19621 ttattttttt ctgctaactc aacaatttct ttgccatcac ttactttcaa agatagtggc
    19681 ttttccacaa aaacattttt accagaaaga attgctttct ttacaaggga ataatgggtc
    19741 tctgctgaac ttgaaataat aactccattt atttctttat ccataaagat ttcatcaaaa
    19801 ttttttgttg tttttacttc attatatttc acctttatat cattaagtgt tctttcatca
    19861 atatcacaaa cagttttgag aactcctaat tcataagaaa ctcttactaa attttttccc
    19921 caccatcctg ctccaattat tcctattttc attttccagt cctttaattt ataaacttcc
    19981 aaattttcct attgttttta tatattgttt ctctgtcttt tattttcgcc ttcttaatcc
    20041 atccctcggc cgtagtccag agggacccgt agccaaaagc aaggaccata cgggcgaggc
    20101 ctttacaaaa ggaaagaata acacttaccc ttaataaaag aaaggggatt tatttcttat
    20161 tcatctattt ttgattttcc ttgaagatat attctaaaac atcttcaact tttatttctt
    20221 tcatacataa cattttatca cattttcttt tataacaggg actacaagca acttttttat
    20281 atatgtaaga gacattaggt aaatttgggc aatgtctctc tggcaaaaat gctccatata
    20341 aacccaatgt tcttattcca agtgctgctc ctatatgaag aggtccacta tcacctgtaa
    20401 ttaaaatttt aacatttgac aatagaaaac aaagattggt aaaatttgtt tttccaacta
    20461 aatttaaggg tttgtatttc atttttttga taatttgatt tacatattca ataccatttt
    20521 tatcacctat tatcacaact ttatacccat ttttaattag aatatctgat agttctgcaa
    20581 atctgtctat accccatctc tttgttttcc atttttcact tgaaagagga tgaatagcaa
    20641 tccagttata attttcatta aaaccaactt ctcttttgaa attttcaaat ttttttttat
    20701 ctgtttcatt aaggtatatt tttggtacct taacataatt ttcagaacta atgtttaagt
    20761 agtttaaaag tagaaattgt ctatcaattg gatttttttc aattatatct tttttattta
    20821 ttccatttgt caaaagtttt ttccctaatt ttgtcctata aaatccatat ctttttcttc
    20881 ccccaataaa gaatgataaa aaatttgttc ttaaattacc attaaggtca aaaataatat
    20941 caaatttttt tctttttaaa ggatatactt taataaggtc taaaataaat ttattggaaa
    21001 ggcatataat ttttttgaaa attgggtagt tttcaaccat ttcattataa attttttttg
    21061 ttataatata tatatctgca tcaggaaaat tttttttcac tgtctgaaaa aaaacagaag
    21121 ataaaattat atcgcctaat gcacctgttt ttattattag aattttttgt ggcatgttag
    21181 atttttaact tgtatgttct tttgtttttt tctattaatt ctcccttttt ttccattttt
    21241 ttcttccttg cctcagttaa aataaaaaaa acactccact cttctttatc tcttacattt
    21301 ctttttcttc ttttactatt attaccaaaa atttctgctc tcaataaaat tgcctttttc
    21361 aaattttcca ttttgaattt atcgaaatta tatttcctca ttttcaattt cctttaagaa
    21421 ctcaattaat ccttcctttt ttgcttgttc ttgtagatat tttttatcta atttttcttt
    21481 atatagtttt aataaaaata tagcccattt tttatcttcg tccgacttcc accatagagc
    21541 agcatttaac ctatcaataa tgatatcttc tatgccttca atatatacct ttaaatcatc
    21601 aataattact tctgaaactt tactgatatc tccactaaga atatcactaa caatatccaa
    21661 ctcaatatct aaattttcat taacccatac tctccctatt tttttaaaat tccattgtaa
    21721 taacaaattt attaccattt ccggaactga tataacaata tcaatatctc cagtggcata
    21781 agaaccaagt gtataaaact caacagcatt accgcctact ataattggtt ttattttttc
    21841 ttttttaagg tattttgtta gtatcgctac aaaatataat cttcttttaa atggttcttt
    21901 tatcctttta agttttttaa attcatctgg aagtttcctc atatggaaat tataaccaaa
    21961 atgataactt atatcaaatt aaatgcctgc cagtcaatac agttgactga caggcacaaa
    22021 agtacaaaga aaaataatta gttattttaa aacaatatcg ttgataaaat aaggatagca
    22081 gagagatata aatggatatt gtaaaatcta aaaataatgt ttctatacgg tctactgaaa
    22141 atggaaaaag aacttttaaa tcaagagata aaaaatgaga ttcttgaaat agcacactat
    22201 cttttaaaat ttccaaagaa aaaatgtgga ttgattatga tgaagaagcg gatgtgttat
    22261 atataaattt caacagacct caaaaagcaa ctgatagcga aatgacaaat gatggtatac
    22321 tgttaagata taaagatgaa gaacttgtag gcataaccat ccttgatgct tctaaaagac
    22381 aaacagagta aatattcgtt taatctcatt catatttaag tagaaaatag ggagaaaaat
    22441 ttctcctgta tccctgtttt aaaaaacaat aatcacctat ctctgcattg ctatcctctg
    22501 ctatcttcac ctgcttttca aagagggaac aattctattc cttccctcgg ccgtagcctg
    22561 taggctacaa cctacgggcg aggccttttg taaaggaagg ttaggatgga tttctttttt
    22621 tgtctttcat ctgtgtcttt gtactttgtc cttgctactc tcaatcaact gttttaacta
    22681 acttataaca actccttgta gacatttaat gtcttttcta ccattttatc aacagaaaaa
    22741 ttttctttta caaatttata tgcattatca gtaatttttt ctattacttc tggattattc
    22801 attaaatatt ttattttttc tgcaattgaa atagaatctt ttggagagac aagaaaaccc
    22861 gttttactat cttcaatcag ttctggtata gcaccaatat tacttgcaat aacaggaact
    22921 ttcgcaaatt gtccctctac aacagttctc ccaaatcctt ctgcctttat tgaaggaata
    22981 acaagtaggt ctatcaattt taagccatct atactattac actttaaaat ttttattttt
    23041 ttacttaaat tatttttttc tatccatttt ttaatttttt tttcataact tttacttcca
    23101 gaaccaataa gaattgtttc aaaatcatat ttattttcat aatataagtc cttaactgct
    23161 tctaaaaagt aaaaccaccc ctttgatttt gtaaatctcc ctatcatccc aataactatt
    23221 ttttctttct tcgttttttc tattctacta aattcatcag atattccatt gtaaaccaac
    23281 ctgattttat ttatatttac tttaagtttt tttattgcgt attcttcaag aaatttactc
    23341 acgactatta ctaaatcacc ttttcctgtt gattggctat atagataaaa tggataaata
    23401 ctatgaatac ttgttacaac aggaatattt tctaacttgc tttttaaact acaaattatc
    23461 caggatggaa acctgctctg aacatgaatt atatctggta tttctttttt aattatttta
    23521 agtagttttt ttcttccttt aaaaaaattt aaaatatttt ttttctccag aggcagatta
    23581 taccacctta tatttttatt ttttataagt tgaggaataa atctaccata accagaaaca
    23641 attataattt catgccctaa ttcactcgcc tttattgaga aatcataaac acctctttca
    23701 attcccccaa aatccattga aggtaaaacc ctcattattt tcatatttca aataaaaatc
    23761 tatgctgttt ttcgtcaaga attaacattg ctgctttaag aacatgttct acatttatcc
    23821 tttttaaaca ttcaaaatca attttacatt tactttcaac tttgcatgga aaacaattca
    23881 ttcctgaatg aaccacctga tttttaactc caattggacc atatctataa ggattacctg
    23941 ggccaaaaat ggcaacaata ggacatccaa ctgctgatgc taaatgcatg gttcctgtat
    24001 catttgtaat aagtaaagaa ctcatttcca atattgctgc aagttctctt atatttgttt
    24061 ttcctgccaa actaaaagca tttttattat caattttttg aataacttca tttgcaagta
    24121 aaatttcatt ttttgagcca actgcaatta tttttatgtc ataatatctg aataactctt
    24181 tccctaattg ggcaaatttg tttttatccc acctttttcc ttcaaaattt gctcccgggc
    24241 ataaaacaac aatattatct gaccttttta tcccatattt taaaagtatt tcttctccct
    24301 tatctttata aactttattt agtgggaaat aaagttgtat atttttttct ctgccaaaaa
    24361 atggttcaat aaaagataaa tttctttctg cctcatgtgt aaatatttta tctgaaaaaa
    24421 actcttttag aaaaaaagtt aatttatatt ttcctcttaa aaaatatggg ataagagaat
    24481 ttctaaaatc aactataagg tcatatctat tttttcttac ttgcattata agttttaata
    24541 accccttctt cttattatac acaaaatatt gtgagaacca tgggttatca atagcaaaat
    24601 caacacttct tggacctatt attacatcaa aagatgcatc aggaaatgca tcttttattt
    24661 tttttattga aggagttaat aataaattat ctcctaaaca acttaaccca attatgagaa
    24721 ttcttcttaa tttatactct ttaatatttt ttggacacat tcttttgcct catttaacac
    24781 tttatacttt ttatttaaaa ctattttaac catatcaggt aatttatcaa tttctgtaaa
    24841 aggaatatat tcagcataac catcttctac aaatttttca atagtcaaat caaaaattag
    24901 tttctttttc ttttttcttt ctactcctaa aattataacc tttttcccag tacttgctgc
    24961 ttctgaaatc atgttaatac tatcttctgt taccataata atatcactta taaaaagtat
    25021 ttcaggatat aatgtctctt ttgaaatggt cggaatttca atataaagaa aatttttatc
    25081 tttcataaaa ttttcacaaa tatattgtat tactttctca ttggttctgc gagaagttgt
    25141 tagaaaaaat tgaaaatttt ctttcatctt atcaatttct tcttttatgt tttttaccca
    25201 gtttgttgaa attctataat tctggtcatc tcctccaatt actattgtta taagtttttt
    25261 tttaattttt tcttttaaag atacaggaat tttatctaat tcatttttct tcatttcctc
    25321 attaattaaa tttggtgctc caagagtaac aattatattc tttctttgta ttttaaattt
    25381 taagaagtca tggtatggaa ttataagaaa gtcaaaaaga gaaaggggca ttccagatgg
    25441 taccattaaa tgaattgaat atgaagatgt ttcttttgat aacagaatat ttaaaggtgc
    25501 aagtaaagaa ccagaagaaa ttataaaatc atattttttt ctactaaatt cttttagatt
    25561 tttagaatat aatttcaata atttattgcc cattttaaac attctaaaaa aacaaaaaag
    25621 gttaaaaaat ttaataaaaa cagggtaatt cccttttctt cctggtaatt tatatcttgg
    25681 tcccttaaac tcaattttta aaatttcaat tttagttgaa ggaaaaacac ttgcaattcc
    25741 atatgactgg acaaaattcc ctttattacc atcatcaagc aaaagtattt taaattcact
    25801 tttcatggtt atgaggtccg tcctcctggg gaggtcggac ctccttattt ttaacgaact
    25861 tcaataccat tttgggctaa atattttttt atttccaata ttgaatattt cctgaaatga
    25921 agaagagatg caagtaaaac agcactcgct ttcccttttt ttacaacatc aactaaatgt
    25981 tgtaaatttc ctgccccacc agaagcaatt acaggtattt taacattttc tgatactttt
    26041 ctcgtaagtt caatatcata tcccctattg gtaccatccc tatctatact tgtaaggaga
    26101 atttctcctg ctcctaattc ctctactttt tttgcccatt cctttacatc aattccagtg
    26161 ggtgttttac cgctttctat atagacctcc caactatctt ttgtgacctt ctttgcatca
    26221 atagcaacaa ctatgcattg acttccaaat tttttaactg cttcacttac taaagatggg
    26281 ttttttactg cggctgtgtt tatagaaatt ttatctgctc ctgatttcaa taattcttca
    26341 atgtctttaa cacaactaat tcctccacca acagttaatg gtataaaaac aacttcagcc
    26401 acctttttaa caacatcaag agttgttttc cttccttcaa ttgttgcgtt tatatcaaga
    26461 aaaacaattt catctgcatt ttcctcacaa taaaattttg cattttcaac agggtcacct
    26521 gcttctctta tattctcaaa atgtactcct tttacaacct tgccctctct tatatcaaga
    26581 cacggtatta tccttactga actcattttg tctcctttgc taatttattt taaggaagtt
    26641 aaaaagaaat tttttagaaa ctgtaatcca tattttccac ttttctccgg atggaattgg
    26701 actccaacta tattattttc aatcactatt gaaggaaatt ctatatgatg ttttgtaatt
    26761 ccataaatta tttctttcct atttacatca gcataataag agtgagcaaa ataaaaatag
    26821 gagttatctg gaatatttga aaataatata ttttttttct cctgttttac tttattccac
    26881 cccatatgag gaactttaat tgatatatca gaaaaagaga attttttaac aataccatca
    26941 aaaattccaa gtccatttaa attaccttct tcactctttt gaaataaaag ttgtagtccc
    27001 aaacatatac ctaaaaaaaa tttacctctt tttatttctg ttttaattac ttctccaatg
    27061 cctgttttta taagaaaatt catcccatcg tcaaaagcac caacaccagg aattacaata
    27121 atccctgctt tcttaatatc cttagaattt tcaattaaaa agggtgtaca accacaaatt
    27181 ttaattgcag aatatatact gaatatattt ccagcaccat aatttattat tccaaccatt
    27241 ttttattgga tgttctcctt cgcatttacc gcttacccac attttctaaa atcctcttgt
    27301 ataaaggaac ttccattagg tcaattttct tttataaaat agaaaaatac cccttcctta
    27361 ttgtttcaag aacccggttc tcacatccaa aggatccctc tgggcgtagc ccaacgggcg
    27421 agccttcgga gagaatcatg ttcttttatc ttaaccggaa aaaatccccc ttcatccccg
    27481 ttgtgctatc tggaaaaatt cccctttata aaatctcccc cacccctctt ttttaaagag
    27541 gggagtaaca ccaactctgc tttgctaatc tccgctactt tagtcggttt accaatgggg
    27601 gaatatcaaa aaccccctca tccctacatt ctccccgcag gggagaagga tgaaatccct
    27661 ctctatctcc ctttattaaa gggagaagaa actccttcct ttgaaaaagg aaggtgggga
    27721 tggattgtct tacacagaag ggtttagagt tttgcttaca gaccatccct acgcagaagc
    27781 gataagttgc gcttacggga ttttatcaaa ttccccaaat ccccctctct ccccctttgc
    27841 taaaggggga acttgtgggt aagggatagt tcctttatta aaggaagaaa caatctactc
    27901 tcattttttc gaagaggata agttattttt tgcctcttac tttgtattta ctatatttct
    27961 ttatgatatt cttgaattgt ttttatattt atttgacttt tctttatctt ttttatagcc
    28021 agaaaagttg cttttgcccc tgggattgtt gtaacaagtg gaatatttct tctaactgca
    28081 attgctctta ttgaaagtat atcttttctt gtttttttcc cagaaggagt attgataatt
    28141 aaatcaatct cattattttt tatataatca agtatatttg gtctttctaa ttcatatatt
    28201 ttaggaatta ttttgacatc tattcctctt tccttcaaat aatttcctgt tcccttactt
    28261 gcaaataatg taaaaccaat ttgagaaaaa ccctttgcaa ttgataaaat agccggttta
    28321 tctttatctt tcacagaaat aaatatattc ccacccgtag gtaatttttg tccagcagca
    28381 agttgacttt tcgcatatgc catctcaaaa ctttcatcta taccaataac ttctcctgtt
    28441 gacttcattt caggacctaa tgtagcatct acattcccaa gccgattaaa tggaaacacg
    28501 gactctttaa cagcaaaatg tttaattttt acctcttttg taaaaccaag ttcttctaat
    28561 gttttgccaa tcataacttt tgttgataat tttgctaatg gaactcctat tgctttactt
    28621 aaaaatggaa ttgttcttga cgctcttgga ttaacttcta aaacatacaa actattatct
    28681 ttaactgcaa attgtatatt tataagtcct ataattttca aataaagcgc aagttttttt
    28741 gttccttcag taatttcctc aattatttct ttttttaaac tgtaaggggg ggttaccatt
    28801 gcactatccc ctgaatgaac tcctgcttcc tctatatgtt ccataattcc accaagaaca
    28861 caaattttac catcggaaat tgcatcaaca tctatctcaa ttgcatcttc aagaaattta
    28921 tcaattaaaa ttggtctatc aggagaaaca tcaactgctt tctccataaa tttctgcaat
    28981 gatacatcat cataaactat ttccattgct cttccaccaa gaacataaga aggtctgaca
    29041 agaacaggat aaccaatttt gttcgcaatt tcttctgctt ctttgaaaca ggttgccgtt
    29101 ccattagagg gctgatttaa tccaacttct ctaagcactg cactaaattt tcttctgtct
    29161 tctgccatat caattgtttc aggacttgtt ccaagaatat ttactccttc atttttaagg
    29221 ggaattgcaa tatttaaagg tgtttgtcct ccaaattgta agataactcc aattggattt
    29281 tccttttgaa caatatttaa aacatcttca acagtcactg gctcaaaata aagtttatca
    29341 gaagtatcat aatcagtact gactgtttct ggattgcagt ttatcataat actctcatat
    29401 ccctcttctc ttaaagcaaa tgaggcatga acacagcaat aatcaaactc aatcccctgc
    29461 cctattctat ttgggccacc gcctaaaata acaactttac ctttgttcat aattaaatat
    29521 attcaattgt acttgggggt tttggagtaa tatcatataa aacttttaca acatctggaa
    29581 ctttttcaac tatttctttc ataacttttt ctaattttga caattggatt ttgggaactt
    29641 ttgcagtaat tgcatccttt gattcaactg ctcttattgc aataatatcc ccatagaccc
    29701 ttttcccact tataattcct gttgctttat cagataacaa aacaggaaaa acctgaaata
    29761 tttttgaact tgtaagtgtt ttttcaacta ttgaagtcgc aattcttact tttctaattc
    29821 tatctggtgt tacctctccg attaccctta ctgaaagacc aggtcctgga aatggttgcc
    29881 tatcataaat ttttttaggg aactttaatg cttttgctaa ttttcttaca tcgggcttgt
    29941 ataattcttt taatggctct ataattttca gtccaaaaga taaaggattt atacctactt
    30001 gcgaaagaac attatgttgt gtcttaattc ccttttgtgt ttcaacaata tctgctgcaa
    30061 tagtcccctg aattaaaaaa tcaacagaat aactcttaac tgcctgtcca agtgttttgt
    30121 aaaaacactc tctaaaagac attcttttct cttccgggtc aattttcccc ttcaatgctt
    30181 caaaaaattt ctcttttaca tcccaaattt caagtgttat accttccttt tcaaaaaaat
    30241 tttttgtttc ttcaatttct ccttctctca taaaaccagt atctaaaagt aatacataaa
    30301 gattatcacc aagtgctctt tttgctataa ttgctgcaac aacactatca actcccccag
    30361 aagtggcaac aagtgctttc tcatttttaa ccactttttt ctttatttca gcaatcttct
    30421 cttttaaaaa tttgtctata ttcatttgcc ctttacctcc ttttatattt tattatattc
    30481 atctcatttt ttaattgaat ttgtttgaaa acacttttct tataagggct taaaactatt
    30541 agaaacagtt gtaattaaac ttatatatat ctcctcttta tcaactagga aatatttact
    30601 caacccggtc tttctgataa aaatatttat acacttttat actatattaa aagcagagtt
    30661 aaagttcaaa tttttttatc aattaaacta taacatatct actatgaatt gacaaatttt
    30721 aattctgttt tcattatact ccccccaagt ttttttcaat tgttctgcag gtccttcata
    30781 tatataggtt ttgatttatt ttcaaaaaaa caaaaagaga cattgatagg tatcagtgta
    30841 ttgacaaaca gaaagaagaa aaacttaaac tctaagaatt tttaccaaat aaacttgatc
    30901 cctaaaatcc cgcaaataac tatgtttttc ttatctgata atatctattg agtaatatga
    30961 tatcttagct tctaaaaact agggctactc taatgaggtt cagttaggat ttacctcatt
    31021 ttttactccc tgttaattgt atgggagaaa cattggtttt acccctccta cagaatttgc
    31081 cctttcataa cttacccatt caacatgccc atcaaaaaaa agacaattcc ctccattatt
    31141 atgtcttcta agacctgacg attcccaagt cttttctggc aatagtagat agggattaaa
    31201 ggtatttgct tgataactat ctgctatcca tacttttgca gatgtgtctt ttactctgtt
    31261 aagttttccc atttttgtta ctggatcgtt tctgattgtt attccataaa atgctgtatc
    31321 taaaggaaaa gcataagaaa ctggacgaac attgggatat ttaaatttct ggcttggaca
    31381 tgtatacaca cagggcttat atagtccatt ggctgttgtt cctaagttat aataatcata
    31441 taccggaaga ttaaaatatt ttgcaagtag aacataccaa gcaggtatat cagcacttgc
    31501 ataaccatca tgttgaccag aaatacctcc accagcaaca taaccacaag gaagccagtc
    31561 atcatagtcc tgacaataca tcattgtcgc agtccctatt tgttttaaat tactcataca
    31621 tactgattgt cttgcttttt ctcttgcctt acttaatgca ggtagtaaca tagcggcaag
    31681 aatcgctatt atcgctatta ccaccagcaa ctcaatcaat gtaaaccctc cgccacaaga
    31741 cacggcggac gctgaaatac gcactctctt tctattcatt tctatcacct cctgttcttc
    31801 tctccaaact cctttccttt ttaatgcctg cccgtctatc tttgtagacg gaaagatgga
    31861 gatggatttc ttcttcactt tatttatata ataactcttt tattttgtca agtccattta
    31921 aaaatcccct ctctatcttt caagaactca gttcttatag agaaccaaag ttcttccatc
    31981 tttacctttt tattttttgt ttcttatttt taattttcac ttggacattt tgctttccaa
    32041 catcccccct catccctcca ccctcgggcg tagtccagag gacgagcctt ctcccttaga
    32101 ggagaaggat gaaatccccc tcttttttcc ccaatgtctg tccgcaatct ttctgtcgga
    32161 ccgctgttca tcggacttaa tatctttaaa accatgccct gattgtattg tatcaacaac
    32221 cgggacttga ttattttcaa ggacccggtt ctcatagaaa accagtccct tctatcttag
    32281 ccgggaaaaa tctcccatcc aaatccccct ccatccccgt tgggtactgc caaaattggc
    32341 tatatctttt ataatgcctt tctttctctt ttaatgcttt tatatgtctt tatatccctc
    32401 accctttttt tatctatttt tttgacacta tctccattcc gtcactgcca gaattttgtt
    32461 acctgttcat ttccccctca cccatacccc tcggccgtag tttagaatcc gtagccaaaa
    32521 gcgaggaccc tacgggcgag ccttctccct ccgccacaaa gcacaacgga cagatgcttt
    32581 aataaaggga gaagaaactc cttcctttaa aaaaggaagg taaggatgga tttctctttc
    32641 atcttttttt tatctatttt ttgacactac ctattaattc actgctttca gtcaaggcag
    32701 ttaactgatt taaattactt ctggccatcc ttttttccat ttcatttcac ctatatatag
    32761 tccttttgtt ctatgttttt tgaggtaaat atcttttata gaacgagaac aactggtaat
    32821 ataccatttc ttctttaaaa aaaatttctc acttgcatga gaaggacaaa gaaaataact
    32881 atctcttcct tcctaatcta atggattatc agaaatagaa aattttgtcc catttccatg
    32941 ccgataaaag aggtaatatc tattagaata cttaattaca caaggagact cagttttaca
    33001 acaagttcct tcataaaatg acttttttct tattaatacc ggacctaaat cttgccaatg
    33061 aataaggtca tttgaagtag caacagcaat gcaggaaaat gttatatctt tttttatatc
    33121 agccacataa tacatcataa aaccatattc tttttcaaaa ataacatgag ggtctctaca
    33181 agaacaagat ttttttatat cccagaaagc ccattctttt gaaggaagaa aaataggatt
    33241 gaaaggatgc ttttcccagt taaacaaatc ttttgaagta gcaaggcaca taaactctgc
    33301 tttttctata ttatgacttg cataaaacat ataaaacttt ttttatgttt aataacataa
    33361 ggagcataaa ttcctctatt ttcccatggt aattgaggat tataaactaa agcatcttct
    33421 actttttgcc acttataaag gtttttgctt atagcatgtc caataattat ttcatttccc
    33481 agatcatagc atcctttacc tacttctcct gaaatataga ataaatgaaa tacttctttc
    33541 tctctaatta tacaatggtc attaacatat tctcctttat tatgatacac atatttaaaa
    33601 gattttggcc atttttttac tggaattatg aatttctttt ccattttatt ttcctccttt
    33661 tcttccccta attataccta attttcactc ctaactttaa ttccttccta tctctttgaa
    33721 tatcattatc ttatccacat gctcatttgg acaaatatta ttgcgacttt tttaagaagg
    33781 aacctaaaat ttcaaacaga ttttattttg agaaaatacg gcatttgtta ttaaattttg
    33841 ttttaaaaat aaagaaattt aatacaaaat cttttggcac ataatatgca gcactaaaaa
    33901 caaatttgtt tcctagaata atttttctat tttgtttaat tcaattatgt aatttgcaat
    33961 ttcttctctt aaattatagt aatcaacagg atctttagaa taattattcc aactttttac
    34021 taatgtatat attttacata atgttatttc tgctttatga acactctctc tttcctttac
    34081 acccaaattt ttattttgat ttaaattatt tatcttttct tctaacataa caaaataatc
    34141 ataatcttct ataccttctc tccataattc ccatcttatt gtggaacacg gtttcccttc
    34201 cccttttaag tttggatata aaagatatcc atctccatct gattttatta tttcccatgg
    34261 attttgtccc catgcattga tagaccagaa taaatatcca tcaaaattat atttatataa
    34321 aatccaaggc ataattcttg aatttaaagc cggctggttt ataagatata aataattatg
    34381 ataaagatat attttttctc ctttcttttg tctcattgtc attaatttta aattatcatc
    34441 aatagaacta gtaggaggac accagacact tatgtaagga ttaagtaaag cattgggaac
    34501 tgaagtcata aaaatcttta aaggagggtt tatatcagat aaaaacttgc agatttcaca
    34561 taaatttttt agtgcttcat aactaggttc atcataaata tatagtaaaa cattatctct
    34621 tattttaatt ttttcaaaaa aatcagataa aatttcatga tactgtttat aatatttttt
    34681 gaaatcttca ctcattattt taattggtat ttctaatgat tctggtctcc atattgcatc
    34741 ggaattccac tttaattgtc ctatatatgt tgaacctgta tttatccatg gacctgcact
    34801 ggtaatgaaa atatctattc cacaatcttt caaaaatttt attctttttt ctaaaacttc
    34861 taaatcttct ttcgaaatat acatattccc attttgaaat tttgcattgg gtaatcctat
    34921 agaaactcct cttatccggt gtgaaacaat atcaggtata taatccttta gaatttcatc
    34981 ccagggtctt ttatcaactt gtaatatggg agaaccccat aaatttgcaa caatttcaag
    35041 gttggttttc tttggtaaag aaaaatccca tactattaat ttaagaggaa ctttatactc
    35101 taacttattt tcaaaattca aaataatctc ccctttatat tccccagaat ttactccttc
    35161 aggaacatat acatttatcc agatagattt actctctcct tcttttagtt ctataaaact
    35221 ttctattggt attaatgggt ctggaatttt cccttttata gaatatgtta taactcccac
    35281 ttcataacaa gaaaaattgt ttttattaat aaaaaccttt gagttctgag aataaaataa
    35341 attcgtgaag tcaatattaa ctttttcaaa atttttataa ggtgttaaaa ctaattgaaa
    35401 aggttcatat tcacctttag cacatttaat aattatttct tcgctcttat ttataggtgg
    35461 tatactttcg ggaaaaactt tttgggtagg tatttcatac catattcgaa cattactatt
    35521 ctcactaaca agggcagatt tagaagatat tgctttttct ttttctgaaa tttgagccaa
    35581 aatttcttca atctttttat tattaactgt taactctaat ttatcaaacc atattgttcc
    35641 attacaacta tatgttgcta attgaattct aaaatattta gcattctgag gaatttgatt
    35701 tacaacaaat tctatttttt gccaatcata agtccctgtt ggtaaatgct tttctgattt
    35761 agaaatccaa cctgctatag attctctcca ccagtccaat cctatataag gtagtcctga
    35821 cacattctct ccttttacat atactgaaat ttttatctcc atctcaggac ctactatcct
    35881 ttttatttct tctatggaaa atggagattg atatccccat ccaaatgttc cctttgcatt
    35941 tgaaatcttt attgactttt tcccttcata aaaagtgttt tcatctattt ctgcaattcc
    36001 ttgtgctttt ccaactttcc agatagtcca attttcggga atgaagtttt cagtaactaa
    36061 ttcaaatgaa aaatttaatt ttttccctat atcttcttta aaggtttcat tgctaaaaag
    36121 cataaaacaa aacaaaaaaa ataaaatttg caccaaaatt attttttctc tcaccggaaa
    36181 cctccctttc attttttcct ctccctattt tttttaacac tacccactgt cattttttaa
    36241 tatcaaatgt gttataatgt actctttacc atccattaaa tcttggatca gaacctaata
    36301 ctcttcctcc taaagtcctt ttatgttccc atccaacatg tccatctaca aaaagaacat
    36361 ttattccatc attatgtctc caaactggat tttctaaccc aatattccac catgggttcc
    36421 aaaattcact ataaaaatcc atagcaagaa atgtactgct tggtcttcta catctatcta
    36481 atttttttaa acgtgcaact tgattcattc cataactata tccattagtc ctcactctat
    36541 cagaaggaca tatccatata taaggttttg gataatccgc tcctttcttt ctatttgttc
    36601 cataaatata agataaaaaa aatgaatttc tacaccaact tgtaaggtct ccaccataag
    36661 aaagaggaaa agtatctggg ggtaaatatc cattaaaatc ctgcgcatat aaataaaacg
    36721 ctattccata ttgttttaag ttattcatac agcttgattg tcttgctttt tctcttgcct
    36781 tacttaatgc aggtagtaac atagcggcaa gaatcgctat tatcgctatt accaccagca
    36841 actctattaa ggtaaaacca gacaccttag aaaatctccc cagacccctc tttactaaag
    36901 aggggaattc caaatatttt cttctcattt tattcacctc tctgtaattt ttaattttac
    36961 tttttttttc atttctatca catccttttt gtttttggtc tcccatcttt cattttacat
    37021 ttcaagaaat ctcccctgac ccccgctgtg cttggaaatt cctcttaaaa tcccccccat
    37081 ccccctttgc taaaggggga aatgacgcac caactctgag gtacaaggta agcaacctta
    37141 gtcggtttgt taaaggggag aactaaaagg gaaaattgaa atataataaa aaaaactcct
    37201 ggtaaaataa atgaatttat aaaagaaaat tttgtaatga ttataaaagg aattgtgatt
    37261 ataaggaaaa aacaaaaaga taccacgaac acgagagagt cctttctctt ggctattact
    37321 tcttctcttt tattacttcc tactattctt ttcatcattt ttcttttttt attatactac
    37381 ccttttttcc tctatcaacc catctttttt tctttttatt atattttacc cttctttttt
    37441 tctgtaaatt gttcttctat attatcccta ctttctatcc tctccattct ttcttttact
    37501 ttttctatat ttgttattga atccacttta tctctcaatc caattatttt tcctcgcaca
    37561 ctatctatct ctttatttca cttcttaatc cacttacatc ttcttccaat ttttcaagtt
    37621 taggagcaat tacttt
//
LOCUS       DAOOVW010000012        37541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_712_length_37541_cov_8.563810, whole genome shotgun sequence.
ACCESSION   DAOOVW010000012 DAOOVW010000000
VERSION     DAOOVW010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 37541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37541
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_712_length_37541_cov_8.563810"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(205..1842)
                     /locus_tag="PLW95_02825"
     CDS             complement(205..1842)
                     /locus_tag="PLW95_02825"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOV21598.1"
                     /translation="MRKINGVVFDFDGTIIHSFIDFGNMKRRIIELCIKYNLKPPSKN
                     LPALELIKEVQKNNKENKNITEFKRESEKIIIEEEIKGMKNAVPYKDILTLIKKLKTK
                     GIKIGIITRNCRKATEIVVKKFSIPYDVFLTRDDVEKVKPSPYHLEKAIEKLGVKKEN
                     TIIVGDHYFDILCGRKAGILTCGIVRDGKFEYFGKEMPDITFESGKEIEYLCGIRKFQ
                     KGKLPLSLLEYLLTRYTYKGKDIIKSSGIGVDASIFKIKNNTIYAKTDPIILVGKDIG
                     TYLININSNDISVAGGNPDYFLSTIIFPQNISFLEIEDIFSQISNECKKHKIKWIGGH
                     TEITPNVKSIISIGFMLGTPIKKQKNEKIKKGDIVFILKEVGIEGASIIVREKYDYLK
                     KIFSEKYLEKVKNSIKNPGIGISEECKFLWENFKIKLMHDPTEGGISTALYELSSVSG
                     HKIIVLFEKIPFYPPACKLCKIFNLNVTGIISSGCIIGITERKEVEKMKKVFKKRKIK
                     FEIIGEIGEKGSGVFIKKKERFIPFPKFEKDEITLLS"
     gene            complement(1851..2216)
                     /locus_tag="PLW95_02830"
     CDS             complement(1851..2216)
                     /locus_tag="PLW95_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21599.1"
                     /translation="MKNFIWIFLMFIFLSGCSIIKENCPTKKKEKKLFNPEGKAEIIK
                     NTSDLTEEGKSAELEKKITYGMDDKQVEKILGNPYGKYISESEMMEVWFYNDFYVGFD
                     KNRKVIIFEIFNPDNSAKY"
     gene            2269..2895
                     /locus_tag="PLW95_02835"
     CDS             2269..2895
                     /locus_tag="PLW95_02835"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HOV21600.1"
                     /translation="MEKEYISPTKVCDIENVDLKMRSEILTKNINTLEEKAINIFNFV
                     REQVKYGFDYPYTKASETLKKGYGNCFNKSSLQISLLRCAEIYSGYIVYLIKKDIFKP
                     IVPPDIYQLISESTIHVSTVVYLNNTWVECDATIDYQLYNAVYKNSGLEYEQWDGKKN
                     LTISHDFIIEQQGIYSNIDLFLLNPPKFWTDELFQKANEYLNKIRGLL"
     assembly_gap    3043..3142
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3143..3590
                     /locus_tag="PLW95_02840"
     CDS             <3143..3590
                     /locus_tag="PLW95_02840"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOV21601.1"
                     /translation="NVLGAIQTKGKGVILTPIYYAFYLYSNNTGDFLLDSEVKVEKYN
                     FQYRNEKIENVPFIDVSATEDEKNIYVAVINRNTEKVDGKIEIENNFKKEGKILTMTG
                     NSPEAINTTEKQEVEIMEENFKKFSDKLNYSFLPYSTTILKIEKNG"
     gene            3583..4731
                     /locus_tag="PLW95_02845"
     CDS             3583..4731
                     /locus_tag="PLW95_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21602.1"
                     /translation="MDKEIEKKLLSFDDKERKAGLYLLKKKIEEGDIKKINIKPYLNL
                     HIHSFHSFNYKNWSPSRIVFEAWINNLEYVGVVDFDTIIAIEETLLSGEILNTKVVSG
                     FESRVFLPELKDKVINSPEEPGIYYLCGKGFKKAIDDNSEEGKFFNKLKEIAQKRNKK
                     VIEKLNNFLVDIKIDYEKNLLPLTPSNNPTERHIIFAYIRKSEEIFGEKVDDFWAGIL
                     KVDKKNIGILRKEKQHDFQEILRKKLIKFGGPGYIKPEVENFPLFDEVVKMTEKVGGI
                     PIGTWLDGTNQGEENPENLIELMKNKGIKGITIIPERNWNIKDPDERKIKIEKLIDFL
                     EVCKKMNMPVICGTEMNKFGQPFIDDFSQPVLSKYLPYFMESASSLFS"
     gene            complement(4728..6113)
                     /locus_tag="PLW95_02850"
     CDS             complement(4728..6113)
                     /locus_tag="PLW95_02850"
                     /inference="COORDINATES: protein motif:HMM:NF014948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HOV21603.1"
                     /translation="MENLKLGFVPIGKFVFSHIDAMKYKKQIEEKLKRWNINYVSMDK
                     ILPDGMVRDQKHVEVVIEFLNKEKVDGVFIPHCNFGTEGAAGMIAKKSGVPVLLWGPR
                     DEEPLEDGTRLRDTLCGLFATSKVLHKLNVPFTYIENCRIEDIAFKEGVEKFMSVISV
                     VKEFKNMRLGQIGSRIDFFWTTIINESELLEKFGIEILPIDIVDFIKQVKENSEKNKR
                     KYIEEFKKTKEKINFSGFENENSILNLFSMRDVMFEIANKEKINAYAIQSFMSICKEL
                     GVMVEFAAAMVSDEGIPVAAETDIHGAISSIILQASSFNKEKVFLADLTIRHPKNDNA
                     VLLWHCSSPISMIDKDCNPEVSNHWVLPGIPSGSCHWKLKDGEMTILRFDGDKGEYKI
                     ICGEGKTVSGPYTKNVYAWLEVKNWKQWEKKFIESPYIHHIATNYGKYTSIIEESVKY
                     LPGNIKFEKLD"
     gene            complement(6101..6883)
                     /locus_tag="PLW95_02855"
     CDS             complement(6101..6883)
                     /locus_tag="PLW95_02855"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOV21604.1"
                     /translation="MKQKKNLATYNKKFEENSYEGIKEAILQKAIVLLPIGQTEEHGR
                     HLPVKTDAFIAEKICEKISEISPVPTIVMPTIYYGYSTKEVTKWPGCPGIKIETFISC
                     IYDICFSLVGMGFKKIVIVSCHGNHTGALRVVVRKIADDCGIYMTLTIPNTIAKEKID
                     KILEKGWRGSCHGCEYETSLMLYLDEKSVDMKKATDIDILKINSEFYPGKVFISTWGL
                     QKSKTGIFGAPVVASKEKGEKIFNSIIEEYIKFLKELSKWKI"
     gene            complement(6852..8201)
                     /locus_tag="PLW95_02860"
     CDS             complement(6852..8201)
                     /locus_tag="PLW95_02860"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOV21605.1"
                     /translation="MKYWQKIDIKEVPDIKVVPPGPKSNEYHQLAGKYMKGYSGQVKL
                     FPVVFEEGNGPFLRDVDGNTYIDFSSGIYVTNCGHCHPKIVEYVKKYLDKLWNCHDFT
                     TPVKTEALQLLSEILPGDINGIQFYSDGTTAIEAGLRAARAITEKFEFISFWGDFHGK
                     TLGSVSLSIMSPDKGIRSPGFFLTPRPYCYRCPFKLHKDSCNLYCVDFVERIIDEETT
                     NRVAAVVMEPIQGWAGSIFPPDEFIPELKKRLEKRGILLFADEILTGMGRTGKWFACQ
                     HYNIVPDIITVGKGLGNGFPVTIMAVREKYKDVLEKISSSSSFGGNPLACASIVATIK
                     VIKEENLIENARKIENVIMEKLYKMKEKHPIIGDVRGKGCIFGIELVKDQHTKEPFEQ
                     AGKLVYQKAFRKGLAWVPAGHILRLSPPLIITEEIAIKGTNIIEEAIYETEKELGYL"
     gene            complement(8230..9483)
                     /locus_tag="PLW95_02865"
     CDS             complement(8230..9483)
                     /locus_tag="PLW95_02865"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOV21606.1"
                     /translation="MSVLVIDEGTTNIKTIIFERDGNIVKQNSIKIKTYYPERECVEQ
                     KAGEWWTCFLKGLKNIDYNKEKIECISSSTQGGTFVLLDKNSKVLTNAFTWLDNRAEK
                     TSKKLKKKFGDDFFYKKTGHPIAAWMPISIISYIREKNPEIYEKVRRISFVADYLNFK
                     LTGRFFLDRTNAQMSSFYNIVEDKWDDDLCEIAGIDKNMLPEIVPSGEIGGKLKKEVA
                     NSLGLKENIPVISGGHDQYCASLGAGVKEKGDCLLSTGTAFALLVLTDKLIFLPKNKK
                     HWKPGRYLFEDKYGVMGPISNGCCILDWIILNFKKFEIKKLNNTKIKFIPYFSEGKGE
                     IKNISLSTKREEIYYSAVRAIVYEIKKYIDEIENKIKVKKFFLVGGGTNISFLPELIK
                     EISKKDVIIPEIKECAARGAFLLTI"
     gene            complement(9480..10496)
                     /locus_tag="PLW95_02870"
     CDS             complement(9480..10496)
                     /locus_tag="PLW95_02870"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOV21607.1"
                     /translation="MLKKTIKLEKNLKIFKEEFEKFLPKKILDFHIHICPKQAVPTGS
                     FAINAGGNKLKKYTFDELKEDMCALYPNREFYGVCFGVPSVNLKTDIMNNYVASVCDK
                     KNFFPLRIVRPEENEKEVEKDIIKKEFYGFKPYRDYAEKFKKKEEVEILDFLPEKILK
                     IADKYGLVIMLHIPKENRLADKSNQKQIMYICNKFPDAKIVLAHIGRAYYFKNIYGNL
                     EKIKKFPNLYFDLAMVNNFEVIEYLFENVAQDKILYGTDIPIALAEGKSVEINDQYTY
                     ITPIPWELSISDEKKKIVFTSFVYEELRAIKKAVERTKKDINFIKNLFFYNGYKLLKE
                     VKIK"
     gene            complement(10498..11883)
                     /locus_tag="PLW95_02875"
     CDS             complement(10498..11883)
                     /locus_tag="PLW95_02875"
                     /inference="COORDINATES: protein motif:HMM:NF014649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21608.1"
                     /translation="MKPKIGLIGLTAQLYKERIPDFIDNLEEFSFELKKVCEEFSSVI
                     HFPVIYKKEQVEEAFKKIRNSDCDGVILVFLSYSPSLIISPVLKKYKDIPVLIWNTQK
                     LFEINENFTDADMLNNHGMHGVQDLASVLLREKTNFSLITGHYKEKQVIEKIKKWCQA
                     TSISKFLKKSKIGRIGGKFKDMGDFSIPDEKINKIIGPEIVDIPLKKLSEISLKIDKS
                     KIEKLMKEDRKNFKIKADEKIHYVVSRIEISLRKIIEEKKLDGVAINFNSFKGQKGCE
                     VIPFSAISKFLSEGIGYGGEGDVICAISVLIFQKLFGEGNFVEMFTTDYKNSRICSSH
                     MGESNINIAKDKGEIEIVEKDMTIVKKGLKTLFFKFQIKPGKITLFNISPTENSFKFI
                     VSKGEIIDIPIFEDITSPHFIFKVGRKVEDFLTEYSKSGGTHHLAMTYGDRSEEIKYL
                     SEIMGIEYFEV"
     gene            complement(11892..13163)
                     /locus_tag="PLW95_02880"
     CDS             complement(11892..13163)
                     /locus_tag="PLW95_02880"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOV21609.1"
                     /translation="MNKKLKVGIFGIRRGDSFANIFNSIPNTEVVAVCDNSEIRVKNF
                     LISGKKNIPTYNDYEKFLQHDMDIVVLCNYCTEHAPAAIKALRSGKHVLSEVIACKTL
                     SEGVSLCREVEKAEKKGIFYMFAENYCYFSYIQEMERIYKAGKIGQLRYGEGEYIHDC
                     SSSWHVLTTSPSHWRNWLPSTYYCTHSLGPIIKITDLRPVQVSGFVVPNLISREYGRE
                     GDDWGILIIKMENDAILRIIPWSIGPHDSIWYRIYGTDGMMENNRWKDTNVLNVHFQK
                     LWEKPKIKSYLPEFKKYQKQAKRSGHGGGDFFLLFDFVNSIRKKERPPIDIYKAMDMT
                     LPGILGYRSALSGNIPIEIPDFRKEKERKKYENDNWSPDPNDKKLMKNQPAPSILGEI
                     EIKKEIYEMLEKKREKAMSEIKKQLEKAYKI"
     gene            complement(13160..14449)
                     /locus_tag="PLW95_02885"
     CDS             complement(13160..14449)
                     /locus_tag="PLW95_02885"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOV21610.1"
                     /translation="MTEKERKIEKFFSYGSPVIVAIDHGQYFGPIEGIESLRKTILKI
                     YNADGILMTPFVFSYIKDFFNSLSSPLVILRVNWTTALCMPWNYKQAHTKLVIEPEEA
                     SFSGADAIIANLTLQSGSEKVDSKNVKVFTEIAKKKEKVGMPLIGEIIPLIPSNEKEK
                     LHTHIKQAVRIAWELGADMIKTIYTGENFNEIIEGVPIPVFILGGDKMKYEEDALDIA
                     KEGAEKGAKGIVFGRNVFQSENPTKFIEQLKATINGNKYSQLKMHLYDFNSLSDTMLP
                     DGYKIKTFKEGNEKIWCEIINKTIGGNLNKEYFEEKFKSKKQFDPDGVFFLYYGDKPC
                     GTVIAWKEDRNLGQLHYLGVLPEHSGKNLGYILCSEVLKYFQKRRINQVFLTTDDFRL
                     SAIKIYLKLGFKPVICEKDNIRRWEKILNYLKLQETK"
     gene            complement(14442..15236)
                     /locus_tag="PLW95_02890"
     CDS             complement(14442..15236)
                     /locus_tag="PLW95_02890"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOV21611.1"
                     /translation="MSLDSEKRKEKIKELIEKRGSIAVSELVRIFNVSEVTIRKELEQ
                     LKKEGILVRTYGGALKSDIIFPENLQFKAKLKKNIREKRIVAEMAIKYIKEGETIFLD
                     SGTTTLEIAKLICEKNLPVTVITNSLPAINYLVNSSIAQLIALSGFFRKESFDFYGPF
                     TIEEIKKLSFTGAFIGVDGISGETGLTTTDVDTARIEEVVIEKAREINIVVDYSKIGK
                     ISPVPYGEMLKKKKDKRIITDYKASKKEIEKLKKYGFEIIIGEKND"
     gene            15591..17075
                     /locus_tag="PLW95_02895"
     CDS             15591..17075
                     /locus_tag="PLW95_02895"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HOV21612.1"
                     /translation="MNIGKLLDEIVVKKGNEECLIFEDKIFKYKDVLEKTNKYANFFK
                     SIGIEKGKKVGIFLQNSPEYIFSYFAIFKMGATGVPIDHRLKVNELCTLLNHSQSEYL
                     ITYNFKDFNADEIKKKVPSIKKIILIDEKVQNTLFIGDSENFPSSFPTRDIDENSIAL
                     ILYTSGTTGTPKGVIWTYKNLESPIESFRYFNLFKDGETTLCCIPFSHNGGIIYPLLI
                     ILGVKLVLMRMYQPLLLLKNLLNWKVDFLFIVPTMLIGILNLKEIDEFLLPDLKWAAV
                     FGAPSSPDILEKFSKVAPNAKLFSGYGLTESSAPNFLHPLDKIKLDSVGMPVPWIEVK
                     IIDKEGKEVKKGEVGELIMKGWPITPGYYNQPDITKEVIKDGWLYTGDIGRFDEDGYL
                     YIVGRKKEMIKVGGLYVFAPEIEGVIYKHPKVKEVAVVGVPDELRGESVKAIIVPKED
                     GITENEIKKFCRQYLASYKIPTIVEFRKELPKTGIGKIKKESLK"
     gene            complement(17889..19067)
                     /locus_tag="PLW95_02900"
     CDS             complement(17889..19067)
                     /locus_tag="PLW95_02900"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOV21613.1"
                     /translation="MEKNVKSSYKMILFFGIVSLCGDIVYEGARSIAGPFLFSLGATA
                     VVVGFVSGIGEILSYGLRFFSGYIADRKKAYWFLTILGYGMIFFIPLLSFAHKLEIAI
                     IFFILERIGKGIRTPARDTILSYASKNVGTGFGFGLHEFLDQIGAVLGPMIFTILLFS
                     NKKYSAAFNILWIPYLILMVILFITKMKYPVPQELEKRTEEKDTNQVKKLPPIFYKYT
                     IFTFFATSGFVNFQLISYHLKFKNVFSLAQIPLLYLIAMGIDGLTAIGIGKIYDKIKL
                     KCLILIPLFSFLIPVFSFTESKLLVYFGIFIWAIVMGIHETIMRAAIADMIPVEKRGL
                     SYGIFNIIYGSGIFIGGVVIGYLYQYVQKLIVPFVLVIEVIAFILFLNLIKKENQIVQ
                     "
     gene            complement(19095..19409)
                     /locus_tag="PLW95_02905"
     CDS             complement(19095..19409)
                     /locus_tag="PLW95_02905"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOV21614.1"
                     /translation="MSIYEKIDNARKLLRLGENATIREIKEAYKNLCLKYHPDRCKER
                     DKKKYEEMIRKLNESMEILLNYCVNYNISFKKEDIKKNTIDYQEYYFETFFDDLWRKN
                     KK"
     gene            19553..21469
                     /locus_tag="PLW95_02910"
     CDS             19553..21469
                     /locus_tag="PLW95_02910"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOV21615.1"
                     /translation="MGRFEKEKLEKDELEKLENLSKICKGDILTMTTLANSGHPGGAI
                     SSLDIFLTTWYFANISPESIDLQERDKIIVSHGHTSAAVYSVLGRLGFFDIDIALSTF
                     RLGGSIFEGHVVRKVPGIEWGTGNLGQGLSAGCGFALSDKIHNRDSYTFVLMSDGEQT
                     KGQVGEARRFAVKYGLNNITVIIDYNKLQISGNIEKIMPCRIYENYKADGWEVIDGVD
                     GHDFNSLYDAIKNSIEIKRPVCIIAQTVMGKGISFMENQHKYHGKPLNEEEYKKAIDE
                     LGISVDLDKYKQLRKKKWEYPERKFYFSPKIEKGNPIIYKEKTDNRTAYGKALDDIAK
                     LNVGNEYPIVVFDCDLSSSVKTDYFEKDFPERFFQVGVQEHNAATIAGAISTDKVISF
                     FSDFGVFGVDETYNQQRLNDQNFTNLKLVCTHLGLDVGEDGKTHQCIDYIGVLRNIFG
                     FKIVIPADPNQTDKVIRYISDKEGNWFVGMGRSKLPIITKENGEIYFGDNYNIQYGKC
                     DLIREGKDGYILTYGCMVYRAIEIVEELKKDGISIGVINFPFPAEIDKEIMEKALKTS
                     FLITYEDHHIDTGLSLTVGIYLSENGYNGKFYRKGVTNYGASGQPDELLKRFGMDIES
                     MVSFIKDKLENKNG"
     gene            21462..21668
                     /locus_tag="PLW95_02915"
     CDS             21462..21668
                     /locus_tag="PLW95_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007043766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21616.1"
                     /translation="MDNCKNKEKNIKSCTCSYPCEKKGVCCECLRSHWINNELPGCLF
                     PPDAEKTYDRSLKYFIKVWSEKLK"
     gene            21671..22798
                     /locus_tag="PLW95_02920"
     CDS             21671..22798
                     /locus_tag="PLW95_02920"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOV21617.1"
                     /translation="MKNRYLLWCIFFFIFLGTSSSQPYINVYLKELGYSPIPLSFVLA
                     SVYFTMAFTEIFAPSILKKLGLKKGIMIGAPASCLFAFFLFAFKNLGIFILGAIIWGF
                     GACILWASVHTMLLKISNENRYGIEIGYLRFFVQVGLLIGSFVLGQIAIISNFRNLFL
                     VAFFFTFSGFLFTFFLEDVKTSFSKFNFNTAFSKKLTIFTIAMFISVFGYGLVVNQLN
                     FFIAEKFSISFLSKIIVLFYLSAGILSFFGGKLIDKTGEIIIPFLFFLFSGLFLIIFS
                     FYQSIPLSIFSIIFIGGLYEIVPIAITVRIGKIIPENKRANTISSTFLWRDFGIGASI
                     FLLGLMRSKFTYQKNFIIMGIIFVLMGIVFIFSFKNEKKFN"
     gene            22779..23489
                     /gene="radC"
                     /locus_tag="PLW95_02925"
     CDS             22779..23489
                     /gene="radC"
                     /locus_tag="PLW95_02925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOV21618.1"
                     /translation="MKKNSIKYWPKDEKPREKLLRDGEHTLSDAQLLAILLRTGTKGE
                     SAVDLAMEIMNKFKSFRNMSHINLKKWEEIKGLGEAKICQIKAAIEIGRRMQEQVLKE
                     KRIKIKNPEDAVKFILPRMRDLKFEIFKVLHLDAKNRIFEMSEIEQGTINQANPIIRN
                     IFQKAIENFSCCLICVHNHPSGDPQPSQEDIEFTNQLKKSGEILGIKVLDHIIIGDNS
                     YYSFASNRQSSFKVSSNE"
     gene            23482..24363
                     /gene="larE"
                     /locus_tag="PLW95_02930"
     CDS             23482..24363
                     /gene="larE"
                     /locus_tag="PLW95_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HOV21619.1"
                     /translation="MNKLYIIPHLKGKIKVEEENRTMEEKIKKLKGILKNLQNVIVSY
                     SGGVDSSFLLEVAVETIGKENVIAVISISDIFTKEEKDFAEKFCASCGVRYIKIKTNQ
                     LQNKNFIKNLPDRCFWCKKELFEKMEKIRRKNKFNYIIDGTNKNDENDYRPGEKAKKI
                     FNVLSPLKEVDITKEEIRKYSKKIGLPTWNKPQMSCLATRIPYGEKITREKLKRIEEG
                     EKFLISLGFKIVRIREHEEIARIEVDKQKIEKLVKMKEKVVKKLKTLGYKYITVDLDG
                     YRSGSMNEVIKISNRAG"
     gene            24742..25008
                     /locus_tag="PLW95_02935"
     CDS             24742..25008
                     /locus_tag="PLW95_02935"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HOV21620.1"
                     /translation="MIKEEKEEMVKTQIILTSEQYKVLRNLAKERDVSMAALIREAVE
                     NAYIKKVDYQEQLEALKRISSRKGDVSDWETMKKEIREGFIGKE"
     gene            25009..25443
                     /locus_tag="PLW95_02940"
     CDS             25009..25443
                     /locus_tag="PLW95_02940"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOV21621.1"
                     /translation="MNIFLDTCIFEYAQGKASIYKNPCLDILQAILDNKITPYTDTEV
                     FQEIWYRHYRRDEDRGIGLLKDTLKIIKQNNILPITYRDITLAIELGKEYGNIIEPRD
                     ALHLAVMLKNHIDIICTVDKGIKAVKEITAIDPIDLEEQFKK"
     gene            25629..25841
                     /locus_tag="PLW95_02945"
     CDS             25629..25841
                     /locus_tag="PLW95_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21622.1"
                     /translation="MEEIKLKVDGYTKFILTVIAVALLGLLFKPLLFSKNLTASQQEI
                     DVNITKIDGYRVSSSFGTPIKVKVVK"
     gene            complement(25971..26372)
                     /locus_tag="PLW95_02950"
     CDS             complement(25971..26372)
                     /locus_tag="PLW95_02950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HOV21623.1"
                     /translation="MKIVFDTNILIAAFISRGVCNELFEHCVCFHEIFISKFILTEFS
                     ENLINKFNFSKYEVNKAVQLLQSRLIIVTPKKLAENISRDPKDDFILATAIQGKCQCI
                     ISGDEDLLILKQYKGIDIVSPQDFWRYEKNK"
     gene            complement(26369..26581)
                     /locus_tag="PLW95_02955"
     CDS             complement(26369..26581)
                     /locus_tag="PLW95_02955"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HOV21624.1"
                     /translation="MRKIITVSIPEDMKKQIDKIAEEEGTTRSNIIKESLQNYLFIRE
                     FRTLRARMMKKSSRVYTDKDIFEQVS"
     gene            27148..27552
                     /locus_tag="PLW95_02960"
     CDS             27148..27552
                     /locus_tag="PLW95_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21625.1"
                     /translation="MVRKRLFMFSLVALFLIVSKSYAGSGWETTGKVLTGVITGDIIK
                     DIPHCHHKCCCKEEYKETYQSYLNTYRVWIPAHWEKKTVHDIIPEGYQTIKVYKEDGS
                     YYYVEKYIPPTIIERTIKIWVEGHWEYKTNRY"
     gene            27574..27717
                     /locus_tag="PLW95_02965"
     CDS             27574..27717
                     /locus_tag="PLW95_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21626.1"
                     /translation="MARKIRKDASLAGLLKRAAIDPKVLKTSSGRKVRKDAKVNTLRK
                     RSK"
     gene            27768..29051
                     /locus_tag="PLW95_02970"
     CDS             27768..29051
                     /locus_tag="PLW95_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21627.1"
                     /translation="MGNFILIVGTSLIIGILLGVILKTKLYRQVQEKNSEEKTLSSQV
                     KERPIKEIPMEIVIKSGNYPDLPPLVFKRTEFNKNIEKQLTDMVNSAKQIIPNAAVSS
                     YAMAQTGHLYKATATNLMKYTNGTYSSILKDLKGQKIAGHAGFLKISPVEVFAPIIVF
                     QAMSIVTGQHYLHEISKQLETIHELLDKLLQLNLTEKVGILLNGYDILNELSSRKTYI
                     REDLIMIHRIRVQAGGIKRQYQTMIKSLDTNNLRKTTSQLSSSKIDDLNNAFKNINQD
                     ITQYSQILMMAERIEVTSRLLEFKIGMANALIKKDDMLLSRTKEILKDIEDSLKYKKF
                     QEIRDKLQQMKNVIAEISEEIKKNFWAVIPSEIEENKRCLENTIQEFTEQLTEHEDAI
                     KKFLQEFLTQINKERELYIVMDEQGNIISPVQIEN"
     gene            29330..30991
                     /locus_tag="PLW95_02975"
     CDS             29330..30991
                     /locus_tag="PLW95_02975"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOV21628.1"
                     /translation="MLDKRAADAFKFLEQIAKFFDSSQTKEVNDRILNQLYSEKGYIP
                     NSKIQITPKMVVDIANILEREVVEPDYFDLSDKKIIEIVEQLQKFYKSDRVILNGKFF
                     DNFVEIVDGITYKEYEKDIENQTCDITPLDYYLKGISYAEKGDYEQALQYYLQGYELI
                     KDEKAKEKLLFCQNILILLICLGKASEIYKWEEEFLGLIEYEKSAHEKVKLHIEIGLS
                     GKIQKNYVIAINHFFLAIDTIKKVFLHGLSGINISGMPCIYESDNKYPIDVLETVLYI
                     KCILEILKDIPDIPEIQEKKQKLLSDMSFIILRMEQIVEKQIFEGSDTSKMKNAHIPL
                     NEKEVKEIQNQIQLYEKDIEKNPDDVFAYFQMGLLYNEREMYEEAYNCFEKALEINPE
                     NIELLFQKGLALASFKKYQEGLVYINKALEIDPKYILALWGKGATLEEMNKGQEADYY
                     YNMALEIEPENIFILTDKGSNLRQLGRYQEAIDTFNKAIAIDPEFAVCYYEKACAESL
                     DNRKDEALKSLKKAIKLDPSLKENAKNDADFKNICEDRTFKRIVR"
     gene            complement(31437..31910)
                     /locus_tag="PLW95_02980"
     CDS             complement(31437..31910)
                     /locus_tag="PLW95_02980"
                     /inference="COORDINATES: protein motif:HMM:NF016834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lecithin retinol acyltransferase family protein"
                     /protein_id="HOV21629.1"
                     /translation="MQIRPNEIKPGSIIKRRISGIFWHMGIYIGDNQVIHFHNPELGH
                     GVDKGIFGKNGATIRMESLKKFAEKEHVVIHTEPKNKKHAQKVIERAKNFYKNPDEYN
                     GKYNLLTKNCEHFATTCFGRDLSPMRQTTKVIISFAVLIITGTVLKSRIGRRNHT"
     gene            complement(32585..36595)
                     /locus_tag="PLW95_02985"
     CDS             complement(32585..36595)
                     /locus_tag="PLW95_02985"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HOV21630.1"
                     /translation="MKRSIWLIFLCCFLVSSSYSAELVGYWKFDEGSGETAWDSADGH
                     PGEIHNAVWTEGKIKGALEFDGKGSYVEVKDSANSCFDLTSMTLSAWIYPKAYPESTP
                     VYWAGIVGKGYYTCSVYGLYLGAHKKLVFSVYGPFGEGGKVGKFTYGENNNSVIKLNQ
                     WSHVVVTYDNYKVKFYINGRLDSVYEETRLPYTNDQSVFIGSVYKDGRYFKGIIDEVK
                     IYRGALTDQEVEEEYQKSGGENTPQSVSIPKIDVPPQIDGNLNDLCWQQARKVDLTEN
                     MTGGPVRCPTTCYICYDDNNIYFGFLCKEPYIDKIKADEKYRDGKVWEDDCVEVFIDT
                     NLDRKSYYHFSLNTLNTQADEKWIDPLCEETGWDGFWDGKTVKGQDFWSAEIAIPFYN
                     FSFSQEASSTWGINLCRERKVEPEYSAWAPTFGGFHKPEMFATVKNINIDFPLYQINP
                     SSSSLTYKLGQTGPGLVFSQKLKNCSHESRKIDVRMKCLTTKQEFKETVSLLANEDKE
                     LNFPISLNEKDTKYKFQLDIFDIISNKRCHLSAKEVKMPSLVSSYFTRDYYTDEKNAK
                     LKNKINIEGILPFGLTASLKVVKGDKTITETTNIPISEADFSVEINIDNLPPGEYMAL
                     LSVYQKESVISLSAIPLIKYTPSIYEIKSDRENLWTLVNNTPFFAIGIMDIPQDKISE
                     YARAGFNITVGPESPEFLDTAYYNGMMVIVSTYQHSAPRDFFSTKPLDELERIIREGS
                     LIPKIKMCKNHPAFFGYFWDEPGEKEAAGVSVEYRITKEYDPYHLVFPCFWEPSGPPI
                     PSDRYDILDTDPYWQPRLGEKITRHVERVDTYWKVANTIRKPFWVTPQADGWSQESKI
                     TSSQQRCQTYLDLIHEARGIVYWEYNIASQDMKETLKKLAGEIKELTPILLTSSPSQA
                     VSGKEKTGIDLLLKLHNEKAYIITANISEDTKKNVQIKLSNIDTNTQGKVLFEKRTVS
                     IVNGAFKDNFEPYATHVYEIGVLSELPDKPIEVNIELLSLEKVPTKEEKTWTKNLDRL
                     PGIKNPGFEEEDSGQPVYWASNLCWEYPEYCSLDSNEHLEGKKSLRMFVSKTTIFSPD
                     TGYGWTKEASSFDLGSKNELLRYGHRFSGIFKVSLDNGEYTVIATMRSPNDISELENG
                     AKHPLKVEEKDIPEIKELFPEYENRIKEYTFGVKIEDGSLDLQTISDGIFGLRIIPKD
                     EEKEEIKFIFTPETAQTKKGYKKVIAPKFPNISVWTEEGAPPVNGGRDYVLSVHLKSD
                     ISDLPVRLKVLDYNWRKHPNPTNKIVNVGPEWKEYTFQTRMHPEQDRARVYIITEGKA
                     GTLWIDKVEVKELDQP"
     gene            complement(36802..37515)
                     /locus_tag="PLW95_02990"
     CDS             complement(36802..37515)
                     /locus_tag="PLW95_02990"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOV21631.1"
                     /translation="MIELLVVIAIIAILAAMLLPALSQAREKARQTSCMNNLKQLGLA
                     VQMYANDYDDWLPLAVNSGGYFVWSCTWVGELMPYLGAPAGQGYEVWSGWTAKVPRNI
                     KKLFICPSGEKERVISVGGVSGGPNYGYNSWVGRYGDVYQYPSNSRYCPKKLNRVRKP
                     ERAFLIADANSTYNKSNNYQFLFISYVMFDAETPYFHYRHNNGANILYVDGHVEWNSK
                     AKIESKRDDHDFWLDISTP"
BASE COUNT        12717 a         6022 c         5487 g        13215 t
ORIGIN      
        1 gaaaatactc cttcccttga caagggaagg aagggatgga ttttcctttt catctttttt
       61 catctaagtt tttgacacta cttcttttta ctatcaaaat tacatttact ttttgtttat
      121 tataatactg gatttttcaa aaaatggcaa gttaattgag taagttttta aaaactttac
      181 taaattttta taccattgtt aatatcagga aagaagggtt atttcatctt tttcaaattt
      241 tgggaaggga ataaaccttt ctttcttttt gataaaaacg ccagaacctt tttcgcctat
      301 ttctccaatt atttcaaatt tgatttttct ttttttaaac acttttttca ttttttcaac
      361 ttctttcctt tcagttattc caataataca accagaagat attattcctg taacatttag
      421 attaaaaatt ttacataatt tacatgcagg tggataaaag gggatttttt caaataaaac
      481 aataatttta tgtccagaaa cacttgaaag ttcataaaga gcggttgaaa ttccaccttc
      541 tgttgggtca tgcatcaatt taattttgaa attttcccat agaaatttac attcttcact
      601 tattcctatt ccagggtttt taattgaatt tttgactttt tcaagatatt tttcactgaa
      661 aatttttttt aaatagtcat atttttctct tacaattatt gatgcacctt caatcccaac
      721 ttcttttaaa ataaatacaa tatctccttt ttttattttt tcattttttt gtttttttat
      781 tggtgtgcca agcataaaac caatagaaat tattgattta acatttggag taatttctgt
      841 atgtccgcct atccatttta ttttatgttt tttacattca ttagaaattt gagaaaaaat
      901 atcttcaatt tcaagaaaac ttatattttg tggaaaaata attgttgaaa gaaagtaatc
      961 tgggtttcca ccagcaacag aaatatcatt actatttata tttataaggt aagtgccaat
     1021 atctttccca acaagaataa ttgggtctgt tttagcataa attgtattgt tttttatttt
     1081 gaaaattgaa gcatcaactc ctattccaga acttttaata atatctttcc ctttatatgt
     1141 atatctggta agaaggtatt caagaagaga aagaggcaat ttcccttttt gaaattttct
     1201 tatgccacaa agatattcaa tttcttttcc actttcaaat gtaatatcag gcatttcttt
     1261 tccaaaatat tcaaatttcc catctctaac aataccacat gtcaaaatac ctgctttcct
     1321 accacataaa atgtcaaaat aatggtctcc aacaattatt gtgttttctt tttttactcc
     1381 taatttttca attgcttttt ctaaatgata tggagatggc tttacttttt ctacatcatc
     1441 tcttgttaaa aaaacatcat aaggaattga gaattttttt acaactattt cagttgcttt
     1501 cctgcaattc ctggtgatta tcccaatttt tattcctttt gtttttaatt ttttaataag
     1561 agtcagtatg tctttataag gaactgcatt tttcattcct tttatttctt cttcaattat
     1621 tattttttca ctttctcgtt tgaattcagt tatatttttg ttttctttat tatttttttg
     1681 tacttctttg attagttcaa gtgcaggaag atttttagac ggaggtttta agttatattt
     1741 tatacataat tcaattattc ttcttttcat atttccaaaa tcaataaaac tatgaataat
     1801 agttccatca aagtcaaaaa ctactccatt aatttttctc atcctttatt tcaatatttt
     1861 gcggaattat caggattaaa aatttcaaat attattactt tcctgttttt atcaaaacca
     1921 acataaaaat cattgtaaaa ccaaacctcc atcatttcac tttcagagat gtattttcca
     1981 taaggattgc ccaatatttt ttcaacctgt ttatcatcca ttccataagt tatttttttc
     2041 tctaattctg cactttttcc ttcttcggtt aaatcagatg tatttttgat aatttctgct
     2101 ttaccttctg ggttaaataa ttttttttct ttctttttgg ttggacagtt ttctttaatt
     2161 attgaacatc ccgaaaggaa aataaacatt aaaaaaatcc aaataaaatt tttcatcttt
     2221 ccctccattt tttgtatttt cttgatttgt tttataatat cataaaaaat ggaaaaagaa
     2281 tatatttccc cgacaaaagt ttgtgatatt gagaatgttg atttaaaaat gagaagtgag
     2341 attcttacaa aaaatatcaa taccttagaa gagaaagcaa taaatatatt caattttgta
     2401 agagaacagg ttaaatacgg atttgattat ccttatacaa aagcatctga aacattgaaa
     2461 aaaggatatg gaaattgttt caataaatca agtttacaga tttctctttt aagatgtgca
     2521 gaaatttact ccggatatat tgtctatctt attaaaaaag atatctttaa accaattgtt
     2581 ccacctgata tttaccagtt gatttctgaa tcaacaattc atgtctcaac tgttgtatat
     2641 cttaataata catgggttga atgtgatgca actattgatt atcaacttta taatgctgta
     2701 tataaaaatt ctggtctgga atatgaacaa tgggatggca aaaaaaattt aacaatcagt
     2761 catgacttta ttattgaaca acaaggaata tattcaaata ttgacctttt tctacttaat
     2821 ccaccaaaat tctggacaga tgaacttttt caaaaagcaa acgaatatct aaataaaatt
     2881 agagggttgc tttaaaataa ctttaatggg ggaaaaaatg aaatgcgaaa ttaaaatcgg
     2941 gaaaaaaagg aaaggaatag taaataaaaa tatatatgga cactttattg aacatcttaa
     3001 aagatgtata tatggcggaa tttatgaaga aaatagttct ttnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnaaatgttc ttggagcaat acagacaaaa gggaaagggg
     3181 ttattttaac accaatttat tatgcatttt acctttattc taacaataca ggtgattttc
     3241 ttcttgatag tgaagttaaa gttgaaaaat ataatttcca gtatagaaat gaaaaaattg
     3301 aaaatgtccc atttattgat gtttctgcaa ctgaagacga aaaaaacatt tatgttgcag
     3361 ttataaatag gaatacagaa aaagttgatg ggaaaataga aattgaaaat aattttaaga
     3421 aagaagggaa aattttaact atgacaggaa attcaccaga agcaataaac acaacagaga
     3481 aacaagaagt agaaattatg gaagaaaatt ttaaaaaatt ttcagataaa ttaaattatt
     3541 ctttcttacc ttactctact acaattttaa aaatagaaaa aaatggataa agaaattgaa
     3601 aagaaacttt tatcatttga cgataaagaa agaaaagcag gtctttatct tttgaaaaag
     3661 aaaattgaag aaggagatat aaaaaaaata aatataaaac catatttaaa tcttcatata
     3721 cactctttcc actcatttaa ctataagaac tggtctccat caagaattgt ctttgaggca
     3781 tggataaata atttagaata tgtcggagtt gttgattttg atacaattat tgcaattgaa
     3841 gagacacttt tatcaggaga aattttaaat acaaaggttg taagtggttt tgaatcaagg
     3901 gtatttttac ctgaacttaa agacaaagtt ataaattctc ctgaagaacc tggtatttat
     3961 tatttgtgtg ggaaaggatt taaaaaggca attgatgata attcagaaga aggaaagttt
     4021 tttaataagt taaaagaaat tgcccaaaag agaaataaaa aagttattga aaaacttaat
     4081 aactttcttg tagatattaa aattgattat gaaaaaaacc ttcttccatt aaccccatct
     4141 aacaatccaa cagaaagaca tataattttt gcttacataa gaaaatcaga agaaattttt
     4201 ggagaaaaag ttgatgattt ttgggcagga attcttaaag ttgataaaaa aaatattgga
     4261 attttgagaa aagaaaaaca acatgacttt caagaaattt tgagaaaaaa acttataaaa
     4321 tttggtggac ccggttatat aaaaccagaa gtagaaaatt ttcctctgtt tgatgaagtt
     4381 gttaaaatga cagaaaaagt tggtggtatt ccaataggaa catggctgga tggaacaaac
     4441 caaggagaag aaaacccaga aaatttaatt gaacttatga aaaacaaagg aataaaagga
     4501 ataacaataa tacctgaaag aaattggaat ataaaggacc ctgatgaaag aaaaattaaa
     4561 atagaaaaac taattgactt tttagaagta tgcaaaaaaa tgaatatgcc tgttatatgt
     4621 ggaactgaaa tgaacaaatt tgggcagcca tttattgacg atttttccca accagttctt
     4681 tcaaaatatc taccatattt tatggaaagt gcttcttctc ttttctctta atcaagtttt
     4741 tcaaatttta tattccctgg caaatacttt acactttctt caataataga tgtatatttc
     4801 ccataatttg ttgctatatg atgaatatat gggctttcaa taaatttttt ttcccattgt
     4861 ttccagtttt ttacttctaa ccatgcatat acattttttg tataaggtcc agatacagtt
     4921 ttcccttcac cacaaattat tttatattct cctttgtccc catcaaacct taatatagtc
     4981 atttctccat ctttaagttt ccagtgacat gaaccagaag gaattcctgg taatacccag
     5041 tgattagaaa cttctggatt gcagtcttta tcaatcatag aaattggaga agaacagtgc
     5101 cagagaagga ctgcattatc attttttgga tgtcttatag taaggtcagc aagaaaaacc
     5161 ttttctttat taaatgaaga tgcctgcaaa ataatagaac ttatggcacc gtgtatatct
     5221 gtttcagcag caacagggat tccttcatcc gataccattg ccgcagcaaa ctcaaccatt
     5281 actcctaatt ctttgcatat actcatgaat gattgaattg cataagcatt tattttttct
     5341 ttattggcta tttcaaacat tacatctctc attgaaaaaa ggtttaaaat agagttctcg
     5401 ttttcaaaac cagaaaaatt tattttttct tttgtttttt taaattcttc aatatacttt
     5461 cttttatttt tttcagaatt ttctttaacc tgttttataa aatctacaat atcaattggt
     5521 aaaatttcaa taccaaactt ctctaaaagt tcgctttcat taataattgt tgtccagaaa
     5581 aaatcaattc tactcccaat ttgtccaagt cgcatatttt taaactcttt cacaacagaa
     5641 ataacactca taaatttttc aactccctct ttaaaagcaa tatcctcaat tctacaattt
     5701 tcaatataag taaatgggac atttagttta tgtaaaacct tgcttgtcgc aaaaagtcca
     5761 caaagagtat cccttaatct tgtcccatct tctaacggtt cctcatctct tggcccccat
     5821 aataaaacag gaacaccact tttctttgct atcatccctg ctgctccttc tgttccaaaa
     5881 ttacaatgag gaataaaaac tccatcaact ttttctttat taaggaattc aataactacc
     5941 tcaacatgtt tttggtctct taccatccca tcaggtaaaa ttttgtccat tgaaacataa
     6001 tttatattcc acctttttaa tttttcttca atttgctttt tgtatttcat tgcatctata
     6061 tgactaaaaa caaatttccc tattggaaca aaaccaagtt ttaaattttc cattttgata
     6121 actcctttaa aaattttata tattcttcaa ttatagagtt aaatatcttt tccccttttt
     6181 ctttacttgc aactaccggt gcaccaaaaa ttcctgtttt gcttttctgt aatccccatg
     6241 ttgaaatgaa aactttccct ggataaaatt ctgaatttat ttttaagata tctatatcag
     6301 ttgctttttt catatcaaca cttttttcat caagatataa cattaaagat gtttcatatt
     6361 cacagccatg acaacttcct cgccatccct tttctaaaat tttatctatt ttttcttttg
     6421 caatggtatt tgggatagtt aaggtcatat aaataccaca atcatcggcg attttcctca
     6481 caactactct taatgctcct gtatggtttc catgacaact aacaataaca attttcttaa
     6541 atcccatccc aactaaagaa aaacatatat cataaataca ggaaataaat gtttcaattt
     6601 taattccagg acaaccaggc cattttgtta cttcttttgt tgaatatcca taataaatag
     6661 ttggcataac aatagttgga actggtgata tttcagatat tttttcacat attttctcag
     6721 caataaatgc atctgtttta accggtaaat gtctaccgtg ttcttctgtt tgaccaatag
     6781 gtaataaaac aattgccttt tgtaatattg cttcttttat tccttcgtaa ctattttctt
     6841 caaatttttt gttataagta gccaagttct ttttctgttt catatattgc ctcctctata
     6901 atatttgttc ctttaattgc aatctcttca gtaattatta aaggtggaga taaccttaaa
     6961 atatgccctg ctggaaccca tgcaagtcct tttctaaatg ctttttgata aacaagtttt
     7021 cctgcctgtt caaatggttc ttttgtatgt tggtctttca ccagttctat accaaaaata
     7081 catccttttc ctctgacatc tccaattata ggatgttttt ctttcatttt gtaaagtttt
     7141 tccattataa cattttctat ttttcttgcg ttttcaatta aattctcctc ttttattact
     7201 tttattgtcg caacaattga agcacaggca agaggattac caccaaaaga agaagaagaa
     7261 cttatctttt ctaacacatc tttgtatttt tctctaactg ccataattgt gactggaaaa
     7321 ccattaccca gtcctttccc tactgtaatt atgtcaggca caatattata gtgttgacag
     7381 gcaaaccatt ttccagttct tcccattcct gttaaaattt catcagcaaa aagtaaaatt
     7441 cccctttttt caagtcgttt ttttaattca ggaataaact catctggtgg gaaaatactt
     7501 cctgcccatc cttgaattgg ttccataaca actgctgcca ccctattagt tgtctcttca
     7561 tctataattc tttcaacaaa atcaacacaa tacaaattac aggaatcttt gtgaagttta
     7621 aatggacatc tataacaata aggtcttgga gttagaaaaa atccaggact tcttattcct
     7681 ttgtcaggac tcatgataga aagagaaaca gaacccaatg ttttcccatg aaaatcaccc
     7741 cagaaagata taaattcaaa cttttcagta attgctcttg ctgctcttaa acctgcttca
     7801 atagcagtag ttccatctga ataaaattgt attccattaa tatcacctgg aagtatttca
     7861 gaaagtagtt gaagtgcttc tgtcttgaca ggagttgtaa aatcatggca attccatagt
     7921 ttgtcaaggt atttttttac atattcaaca atttttggat gacagtgtcc acaattagta
     7981 acatatattc cagaagaaaa atcaatataa gtatttccat caacatctct taaaaacgga
     8041 ccattacctt cttcaaatac aactggaaat aatttaacct gtcctgaata ccctttcata
     8101 tatttacccg caagttggtg atattcattt gattttggac caggtggaac aacttttata
     8161 tccggtactt cttttatatc aatcttttgc cagtacttca ttttccgcct cctatttttt
     8221 gatattatgt taaattgtta ataaaaaagc acctctggca gcacattctt ttatttcagg
     8281 aattattaca tctttttttg aaatttcttt tatgagttca ggtaaaaaac taatatttgt
     8341 tcctcctcca acaagaaaaa atttctttac ttttatttta ttctctattt catctatata
     8401 cttctttatt tcataaacaa ttgcccttac tgctgaataa tatatttctt ctctttttgt
     8461 tgataatgaa atgtttttaa tttctccttt cccttctgaa aaatatggaa taaattttat
     8521 ctttgtatta tttaattttt taatttcaaa ttttttgaaa ttcagaataa tccagtcaag
     8581 aatacaacaa ccatttgaaa taggtcccat tactccatat ttatcttcaa ataaatatct
     8641 ccctggtttc cagtgttttt tatttttagg caaaaaaatt aatttatcag ttaaaacaag
     8701 taaagcaaaa gcagtcccag ttgaaagtaa gcaatcccct ttttctttca caccagcacc
     8761 aagtgaagca caatactggt catgtccccc tgaaataaca ggtatatttt cttttaaacc
     8821 aagagaattc gcaacttcct tttttagttt gccaccaatt tctccagaag ggacaatttc
     8881 aggtagcata tttttatcta tccctgcaat ttcacataaa tcatcatccc atttatcttc
     8941 aacaatatta taaaaagaac tcatctgtgc atttgttcta tctaaaaaaa atcttcctgt
     9001 taatttgaaa tttaaataat cagcaacaaa agaaattctt cttacttttt cataaatttc
     9061 tggatttttt tctcttatat aagaaattat agaaattggc atccatgctg caataggatg
     9121 acctgttttt ttataaaaga aatcgtcacc aaactttttc tttaattttt tagatgtttt
     9181 ctctgctcta ttatcaagcc atgtaaaagc atttgttaat acctttgagt ttttatcaag
     9241 aagaacaaat gttcctcctt gtgttgaact tgatatacac tcaatttttt ctttattata
     9301 gtcaatattc tttagccctt ttaaaaaaca agtccaccat tcccctgctt tttgttcaac
     9361 acattctctt tcgggataat atgtttttat ttttatagaa ttttgtttta ctatattacc
     9421 gtctctctca aaaataattg tttttatatt tgtagttccc tcatcaatta caagaacact
     9481 cattttattt tcacctcttt aagaagtttg tatccattat aaaaaaacaa attttttata
     9541 aaatttatat ccttttttgt tctttcaact gctttcttta ttgctcttaa ttcctcataa
     9601 acaaaagaag taaatacaat ttttttcttc tcatcagaaa ttgataattc ccatggtatg
     9661 ggagttatat atgtgtactg gtcatttatt tcaactgatt ttccttctgc aagagcaatt
     9721 ggtatatctg tcccataaag aattttatcc tgtgcaacat tttcaaaaag atattctata
     9781 acttcaaaat tatttaccat tgcaaggtca aaatataaat tggggaattt ttttattttc
     9841 tcaagattcc cataaatatt tttgaaataa tatgcccttc caatatgagc aagaactatt
     9901 tttgcatctg gaaatttatt acatatatac ataatttgtt tttgattgga tttgtctgct
     9961 aacctgtttt cctttggaat atggagcata ataaccaacc cgtatttatc tgcaattttt
    10021 aaaatttttt caggtaaaaa gtcaagaatt tcaacttctt cttttttctt aaatttttca
    10081 gcatagtccc tatatggctt gaaaccataa aactcctttt ttataatatc tttttcaact
    10141 tctttttcat tttcctctgg tcttacaatt cttaatggga aaaagttttt tttatcacaa
    10201 acagaggcaa cataattatt cataatatct gtttttaaat taacacttgg gactccaaaa
    10261 caaacaccat aaaattccct atttgggtaa agagcacaca tatcttcttt caattcatca
    10321 aaggtatatt ttttgagttt atttcctcct gcatttattg caaaactacc agtgggaact
    10381 gcctgttttg gacatatatg aatatggaaa tcaagaattt ttttaggcaa aaatttctca
    10441 aattcttcct taaatatctt caaatttttc tctaatttta ttgttttttt caacatttca
    10501 aacctcaaaa tattcaattc ccataatttc tgaaagatac tttatttctt ctgacctatc
    10561 accataagtc atggcaagat gatgtgttcc tcctgattta ctatattcag ttaaaaaatc
    10621 ttcaactttc ctgcctacct taaatataaa gtgaggagaa gtaatatctt caaaaattgg
    10681 tatgtcaata atttctcctt ttgaaacaat aaatttaaaa gaattttctg tcggagatat
    10741 attgaaaaga gttattttgc ctggttttat ctggaacttg aaaaaaagag ttttaagtcc
    10801 ttttttcaca attgtcatat ctttttcaac aatttctatt tctcctttat cttttgcaat
    10861 gtttatattt gactctccca tatgagaaga acatattctg ctgtttttgt agtcagttgt
    10921 gaacatttca acaaaatttc cttctccaaa gagtttttga aaaattaaaa cagaaatagc
    10981 acatataaca tctccttctc ctccataacc aattccttct gataaaaatt ttgaaattgc
    11041 agaaaaagga attacttcac agcccttttg tcctttaaaa gaattaaaat ttattgcaac
    11101 accatctaat tttttttctt ctattatttt tcttaaagaa atttcaattc ttgatacaac
    11161 ataatgaatt ttctcatctg cctttatttt gaaatttttt ctatcttcct tcattaactt
    11221 ttctatttta gatttgtcta tttttaaaga aatttctgat agttttttaa gtggaatatc
    11281 aactatttct ggaccaatta ttttatttat tttctcatca ggaatagaaa aatctcccat
    11341 atctttaaat tttccaccaa ttcttcctat ttttgatttt tttaaaaatt ttgatattga
    11401 agttgcctga caccattttt taattttttc tataacttgt ttctctttgt aatgtcctgt
    11461 aattagagaa aaatttgttt tttctcttaa taaaacactt gctaaatcct gaaccccatg
    11521 cattccatgg ttgttaagca tatctgcatc agtaaaattt tcatttattt caaaaagttt
    11581 ttgtgtattc catattaaga caggtatgtc tttatatttt tttagaacag gagaaataat
    11641 taaacttggg ctatatgaaa gaaaaacaag aattactcca tcgcaatcag agtttctgat
    11701 tttcttaaat gcctcttcca cttgttcttt tttataaata acagggaaat ggataactga
    11761 agaaaattcc tcacatactt ttttcaactc aaaagaaaat tcttctaaat tatctataaa
    11821 gtcaggtatc ctttccttat agagttgtgc cgttaatcca attagaccta ttttgggttt
    11881 cataagttat cctaaatttt atatgctttt tctaattgtt ttttaatttc cgacattgct
    11941 ttctctctct ttttttcaag catttcataa atttcttttt taatttcaat ttctccaagg
    12001 atagaaggtg ctggttggtt tttcattagt tttttatcat ttgggtcagg tgaccaatta
    12061 tcattttcat atttttttct ttctttttct tttctaaaat ctggtatctc aataggaata
    12121 ttaccagaaa gagcagaacg ataccccaaa attccaggca atgtcatatc cattgcttta
    12181 taaatatcta ttggtggtct ttcttttttc cttattgaat tgacaaagtc aaataaaagg
    12241 aaaaaatctc ctcctccatg tcctgacctt tttgcctgtt tttggtactt tttgaattca
    12301 ggaagataac tttttatttt tggtttttcc cataatttct gaaaatgaac atttagaaca
    12361 tttgtatctt tccacctatt attttccatc atcccatcag ttccataaat tctgtaccag
    12421 attgaatcat ggggaccaat actccaggga ataattctta aaattgcatc gttttccatt
    12481 tttataatga gtattcccca atcatcacct tctcttccat attctctgga tatcaaatta
    12541 gggacaacaa atccagatac ctgaacaggc cttaaatcag ttatttttat tattggacca
    12601 agagaatgag tacaataata agttgaagga agccagtttc tccaatgaga aggtgaagta
    12661 gttaaaacat gccaggaaga agagcaatcg tgaatatatt caccttctcc atatcttaat
    12721 tgcccaattt ttcctgcttt gtaaatcctc tccatttctt ggatatagga gaaataacaa
    12781 taattttcag caaacatata aaatatcccc tttttttctg ctttctctac ttctctacaa
    12841 agagagaccc cttctgagag cgttttacag gcaattactt ctgataaaac atgctttcca
    12901 cttcttaatg cttttattgc tgcgggagcg tgttctgtgc aataattaca aaggacaaca
    12961 atatccatat catgttgcaa aaacttttca taatcattat aagtaggaat gttttttttc
    13021 ccacttatta aaaaattttt aactctgatt tcgctattat cacagacagc aacaacttct
    13081 gtatttggaa tagagttaaa tatgtttgca aaactatcac ctcttctaat accaaaaatc
    13141 ccaactttta attttttatt cattttgtct cctgtaactt taaataattt aatatttttt
    13201 cccatcttct tatattatct ttttcacaaa ttactggttt aaaaccaagt tttaaataaa
    13261 ttttaatagc agacaatcga aaatcatctg ttgtaagaaa tacttgattt attcttcttt
    13321 tttggaagta ttttagaact tctgaacata aaatatatcc aaggttttta ccagaatgtt
    13381 ctggaagaac acctaaatag tgaagttgtc ctaaatttct atcttctttc catgctatta
    13441 ctgtcccaca cggtttatct ccataataaa gaaaaaatac cccatcaggg tcaaattgtt
    13501 tttttgattt gaatttttct tcaaaatatt ctttattcaa attcccacca attgttttat
    13561 ttattatttc acaccatatt ttttcatttc cttctttaaa tgtttttatt ttatatccat
    13621 ctggaagcat agtatcagaa agtgagttaa aatcataaag atgcattttt aattgactat
    13681 atttattccc atttatagtt gcctttaatt gttcaataaa tttagttggg ttttccgatt
    13741 ggaagacatt tctaccaaaa acaatacctt ttgcaccttt ttctgcccct tctttagcaa
    13801 tatctaaagc atcttcttca tatttcattt tgtctccacc aagtataaaa acaggaatag
    13861 gaactccttc aataatttca ttaaaatttt ctcctgtata aattgttttt atcatatctg
    13921 ccccaagttc ccatgcaatt ctaactgcct gttttatatg agtatgtagt ttttcttttt
    13981 catttgaagg aataagaggt attatttctc caattagtgg cattccaact ttttctttct
    14041 ttttggctat ttcagtaaaa actttaacat ttttagagtc aactttttca cttccactct
    14101 gtaatgtcaa atttgctata atagcatctg caccagaaaa agatgcttct tctggttcaa
    14161 tgactaattt tgtatgtgct tgtttataat tccatggcat acataaagca gttgtccaat
    14221 ttactcttaa aataactaat ggagatgata atgagttaaa aaaatctttt atataagaaa
    14281 aaacaaaagg agtcataaga attccatccg cattataaat tttaagaatt gtctttctta
    14341 aagattcaat tccttcaata ggtccaaaat actgcccatg gtcaattgct actattacag
    14401 gactcccata tgaaaaaaat ttctctattt tcctttcttt ctcagtcatt tttttctcct
    14461 attatgattt caaaaccata tttttttaat ttttcaattt ctttttttga tgctttataa
    14521 tcagtaataa ttcttttgtc tttctttttt ttcaacattt ctccataagg aactggtgaa
    14581 atttttccaa tttttgaata gtcaacgaca atatttattt ctctcgcttt ttcaattact
    14641 acttcttcaa ttctcgctgt atcaacatca gttgttgtca gcccagtttc acctgatatt
    14701 ccatccactc caataaaagc acctgtaaat gaaagttttt ttatttcttc tattgtaaaa
    14761 ggtccataaa aatcaaaact ttcttttcta aaaaaaccac ttaaagcaat aagttgggct
    14821 atagatgaat taacaaggta atttatcgct ggtagagaat tcgtaataac tgtaacaggt
    14881 aaattttttt cacaaattaa ttttgcaatt tctaatgttg ttgttcctga atcaagaaat
    14941 attgtttctc cttcttttat atactttata gccatttccg caactattct tttttctctt
    15001 atattttttt tcaattttgc cttgaattgg agattttcgg gaaatataat atctgatttt
    15061 aatgcccctc catatgttct tactaaaatt ccttcttttt tcaattgctc aagttctttt
    15121 ctgattgtaa cctcagatac attaaaaatt cttacaagtt ctgaaacagc aatactacct
    15181 cttttttcaa ttaattcctt tattttctct ttgcgttttt ctgaatctaa actcattttg
    15241 ttttctttct tttttttatg ttttatttcg ttttattaca tttaatttac actttaaaaa
    15301 tttttttgtc aaatttattt ttacttacaa gaaatttagc actctttctc agactttttt
    15361 aagataaaaa atgttgtatt tgcaactcta tcattatttt ttattcccag ttttatattt
    15421 tctccagttt ttactggcaa ttttttgaaa atattttcta cctaatacaa ttctacttta
    15481 tatattatcc tctatttctg taaaacattt ccaataatat gaattaaaaa aggaaatttg
    15541 atgttcttat tttttttgtt aaaataataa aaatcacata ggagaagaaa atgaatatcg
    15601 gtaaattgtt agatgaaatt gttgtaaaaa aagggaatga agaatgtctt atatttgagg
    15661 ataaaatttt caaatacaaa gatgttttag aaaaaacaaa taaatatgca aattttttca
    15721 aaagtatagg gattgaaaaa ggaaaaaaag ttggaatttt ccttcaaaac tcccctgaat
    15781 atattttttc ttatttcgca atttttaaaa tgggtgcaac aggagttcct attgaccacc
    15841 gacttaaagt taatgaacta tgcacacttc taaatcatag ccagtcagag tatcttataa
    15901 cttataattt taaggacttc aatgcagatg agataaagaa aaaggtacct tcaattaaaa
    15961 aaataatcct tattgatgaa aaggttcaaa atacactttt tataggagat agtgagaatt
    16021 ttccttcttc ttttcctaca agagatattg atgaaaattc aattgcttta attctttata
    16081 cttctggaac aacaggaact ccaaaagggg ttatatggac atataaaaat cttgaaagtc
    16141 caatagaatc atttagatat tttaatcttt tcaaagacgg agagacaact ttatgctgta
    16201 tccctttttc tcataatgga ggaattattt atcctctttt gataattctt ggagttaaac
    16261 ttgtccttat gagaatgtac cagccacttt tactgctaaa aaatctttta aattggaaag
    16321 tggactttct ttttattgtt ccaacaatgc ttatcgggat attaaaccta aaagaaattg
    16381 atgagttttt attacctgac cttaaatggg ctgctgtttt tggtgctcca agttcccctg
    16441 atatacttga gaaattcagc aaagttgctc caaatgcaaa acttttttct ggatatggtc
    16501 ttacagaaag ttctgcacca aatttcttac atcctcttga taaaataaaa ttggattcag
    16561 tcggcatgcc tgttccgtgg attgaagtaa aaataattga taaagaaggg aaagaagtaa
    16621 aaaaaggaga agttggagaa ctaataatga agggctggcc aataactcct ggttattaca
    16681 atcagccaga tattacaaag gaagtaatta aagatgggtg gttatacaca ggagatattg
    16741 gaagatttga tgaagatggg tatctttaca tagtaggcag gaaaaaagaa atgataaaag
    16801 ttggtggact ttatgtattt gcacctgaaa ttgaaggagt tatatataaa catccaaaag
    16861 ttaaagaagt agcagttgtt ggtgttccag atgaattaag aggagaaagc gtgaaagcaa
    16921 ttatagtccc aaaagaagat gggataacag aaaatgaaat aaaaaaattc tgtagacaat
    16981 accttgcttc ttataaaatt ccgacaattg ttgaatttag aaaagaacta ccaaaaacag
    17041 gaatagggaa aataaaaaaa gaatctctca aataacctaa gttatcagta aaaatttggg
    17101 cagtttgtgt cagcaagtat aaatgaaagt catataggga aatcccgtaa gcataactta
    17161 tcgcttcggc acctgcccgt ctgagcatta gcaaaggagg gtagggatgg tctgtaagca
    17221 aaactctaaa cccttctgtg taagacaatc cattccatcc tcccccttct ctcggccgta
    17281 gctagtaggc tacagcctat gggcggggtc tttttcaaag aagggagcca aacgtttccc
    17341 cttttgtaaa ggggatagag ggggatttga agggaaaatt taatataaat ccatcccggc
    17401 cttcctttga taaaggaagg agagatactc ccctctttga aaaacgacta aagttgctta
    17461 ccttgtgcct cagagttggt gcttcatcgt ctcccacttt ataaaaggtt aagatagaag
    17521 aacatggttc tctgtgagaa ccgggttctt gaaacaataa ggtaagtaag tatattcttc
    17581 cctttgacaa agggaagtta gaaaggattt ttagcggggg gatttaataa aagccagccc
    17641 taccctcctt tgctaatgct cagacgggca ggcgcagaag caataagttg agcagacggg
    17701 gtttccagat agcacaacgg gagtttaaga gagatttata ataaaaatcc acccccaccc
    17761 tcctttttta aaggagggag ataaaggaga agaagtcccc gcttcgccat ttcccccttt
    17821 ctaaaagggg gagacagggg gatttgaaag ggagatttca gcaagcacaa cgagagatta
    17881 agagggattt attgaacaat ttgattttct ttcttgatta aattgagaaa aagaataaaa
    17941 gcaataactt caattacaag aacaaaagga acaataagtt tttgcacata ttgatataaa
    18001 tagccaatta caactcctcc aataaaaatg cccgaaccat aaattatatt aaatatccca
    18061 taagacaatc cccttttttc aacaggtatc atatctgcaa ttgctgccct cataattgtt
    18121 tcatgtattc ccattacaat tgcccagata aaaattccaa aatatacaag taatttgctt
    18181 tctgtgaaag agaaaacagg aataaggaaa ctaaataaag gaattaaaat cagacatttc
    18241 aattttatct tatcataaat tttcccaatt cctattgctg taagaccatc aattcccata
    18301 gcaataagat aaagcaaagg aatttgagca agagaaaaaa catttttgaa ttttaaatga
    18361 taggaaataa gttgaaaatt aacaaaacca gatgtagcaa aaaatgtaaa aatagtatat
    18421 ttataaaaaa ttggaggtaa tttttttact tgatttgtat ccttttcttc tgttcttttt
    18481 tcaagttcct gtgggactgg atatttcatt tttgttataa aaagaataac cataaggata
    18541 agatatggaa tccacaaaat attaaaagca gcagaatatt ttttattact gaaaagaagt
    18601 atagtaaaaa tcattggtcc taaaacagca ccaatctggt ctaaaaattc atgaagtcca
    18661 aatccaaaac cagttcctac attttttgaa gcataagaaa gaattgtatc tcttgcaggt
    18721 gttctaattc ctttgccaat tctttcaagg ataaagaaaa ttattgctat ttctaattta
    18781 tgagcaaagg aaagaagagg aataaaaaaa atcattccat atcccaaaat tgtaagaaac
    18841 cagtatgctt tttttctatc tgctatgtat ccagaaaaaa atctcaatcc ataacttaaa
    18901 atctctccaa ttcctgaaac aaaacccact acaactgctg ttgcaccgag agaaaataga
    18961 aacggtcctg ctatacttct ggcaccttca taaacaatat ctccacataa actaacaatc
    19021 ccaaaaaaca aaatcatttt atacgaactc tttacatttt tttccattta ttcattataa
    19081 atttattttt gttttcactt tttatttttt ctccataagt catcaaaaaa tgtctcaaaa
    19141 taatattcct gataatctat tgtatttttc tttatatctt cttttttaaa agaaatatta
    19201 taattaacac aataattcaa tagtatttcc attgattcat ttaatttcct tatcatttct
    19261 tcatatttct ttttgtctct ctctttacac ctatctggat ggtattttaa gcataaattt
    19321 ttatatgctt cttttatttc tctaattgtt gcattttccc caagtcttaa caatttcctg
    19381 gcattatcaa tcttttcgta tattgacatt tatctcctcg ttttaattta ttatatgtgt
    19441 tctacacaat tatataaatt ttctcatcaa aatatctttt tacaaaattt atctcaaaaa
    19501 tttggaataa aatttctggt atgttaaaat tatttttcaa aaaggagaag aaatgggaag
    19561 atttgaaaaa gaaaaattgg aaaaggatga attagaaaaa ttagaaaatt taagtaagat
    19621 atgtaaaggg gatattttaa caatgacaac tcttgcgaat tcaggacatc ccggaggagc
    19681 aatttcatct cttgatatat ttttaactac atggtacttc gcaaatatct ccccagaaag
    19741 tattgattta caagaaagag ataaaattat tgtaagtcat gggcatacat ctgctgctgt
    19801 ttattctgtt ttagggcgat taggattttt tgatatagac atcgcacttt caactttccg
    19861 acttggtgga agtatttttg aaggtcatgt tgtccgaaaa gttccaggta ttgagtgggg
    19921 aacaggaaat ttaggacagg gactttctgc tggttgtggt tttgctttat ctgataaaat
    19981 tcataataga gatagttata catttgtttt aatgagcgat ggagaacaga caaaaggaca
    20041 agtaggagag gcaaggaggt ttgcagtaaa atatggtctg aataacatta cagttatcat
    20101 tgattataat aaattacaaa taagtggaaa tattgaaaaa attatgccat gtagaatata
    20161 tgaaaattat aaagcagacg gatgggaagt tattgatggt gttgatgggc atgattttaa
    20221 ctctctttat gatgcaatta aaaattcaat tgaaattaaa agacctgttt gtataattgc
    20281 tcaaacagtt atgggaaaag gtatttcttt tatggaaaat cagcataaat atcatggaaa
    20341 accattaaat gaagaagaat ataaaaaagc aattgatgaa ttgggaattt cagttgattt
    20401 agataaatat aaacaattaa gaaagaaaaa atgggaatat ccagaaagaa agttttattt
    20461 ctctccaaaa attgaaaaag gtaatcccat tatatataaa gaaaaaacag ataataggac
    20521 tgcctatgga aaagcgcttg acgatatagc aaaattaaat gttggaaatg agtatccaat
    20581 tgttgttttt gattgtgact tatcttcaag tgtaaaaaca gattattttg aaaaggactt
    20641 tcctgaaaga ttctttcagg ttggagttca ggaacataat gctgctacca ttgcaggagc
    20701 aatttcaact gataaggtca taagtttctt ttctgatttt ggtgtttttg gagttgatga
    20761 gacatataat caacaacggc ttaatgacca gaattttaca aacttaaaac ttgtctgcac
    20821 tcatttaggc cttgatgtag gagaagatgg gaaaacgcat caatgtattg attatattgg
    20881 agttttaagg aatatttttg gatttaaaat agtaatacca gcagacccaa atcaaacaga
    20941 taaagttata agatatattt cagataaaga gggaaattgg tttgttggaa tgggaaggtc
    21001 aaaattacca attattacaa aagaaaatgg ggaaatttat tttggagata actacaatat
    21061 tcaatatgga aaatgtgatt taataagaga aggtaaagat ggttatattt tgacttatgg
    21121 atgtatggtt tatagagcaa tagaaatagt tgaagaatta aaaaaagatg gaatttctat
    21181 tggagttata aatttcccat ttccagcaga aattgataaa gaaattatgg aaaaagcatt
    21241 aaaaacatct tttttaatta cttatgaaga ccaccatata gatacaggac tttctttgac
    21301 agttggaatt tacctttctg aaaatggata taatggtaaa ttttacagaa aaggagttac
    21361 aaattatggt gcttcaggac aaccagatga attgttaaaa agatttggaa tggatattga
    21421 aagtatggtt tcttttataa aagacaaact tgaaaataaa aatggataat tgtaaaaaca
    21481 aagaaaaaaa tatcaaaagt tgtacctgtt cttatccatg tgagaaaaaa ggtgtttgtt
    21541 gtgaatgttt aaggtcccac tggataaata acgaacttcc tggatgtctt ttcccaccag
    21601 atgcagaaaa aacttatgat aggtctttaa aatattttat aaaggtctgg agtgaaaaat
    21661 taaaataaat ttgaaaaaca gatatttatt atggtgtatt ttctttttta tatttttagg
    21721 gacgagttct tctcaaccat atatcaatgt atatcttaag gaattaggtt attctccaat
    21781 tcctctttca tttgttcttg caagtgtcta ttttacaatg gcttttactg aaattttcgc
    21841 tccatctatt cttaaaaaat taggtctgaa aaaaggaata atgattggcg ctcctgcctc
    21901 ttgtcttttt gctttttttc tttttgcctt taaaaattta ggtatattta ttttaggtgc
    21961 tattatttgg ggatttggag catgcattct atgggcatct gttcacacaa tgcttcttaa
    22021 aatttcaaat gaaaatagat atggaattga aattggatat cttcgttttt ttgttcaagt
    22081 tgggctttta attggttctt ttgttttagg acagattgcc ataataagta attttagaaa
    22141 tttattttta gtcgctttct tttttacttt ttctggtttt ttatttacct tctttttaga
    22201 agatgtgaaa acatcttttt caaaatttaa ttttaatact gcattttcaa aaaaattaac
    22261 aatttttacc attgctatgt ttataagtgt ttttggatat ggtttagttg tcaaccaact
    22321 taattttttt attgcagaaa aattctcaat ttcttttctc agtaaaataa tcgtcctttt
    22381 ctatctttca gcaggaattt taagtttttt tggtggtaaa ttaattgaca aaactggtga
    22441 aattattata ccttttcttt tcttcttatt ttcaggactc tttttaataa ttttttcttt
    22501 ttatcaatca attcctttat caattttttc aataattttt ataggtgggc tatatgaaat
    22561 tgttcctatt gcaataacgg taagaattgg taaaattatc ccagaaaata aaagagcaaa
    22621 tacaatctct tccacttttt tatggagaga ctttggaatt ggggcaagca ttttcctttt
    22681 aggtcttatg agaagtaaat ttacttatca gaaaaatttt attataatgg gaattatatt
    22741 tgttctaatg ggtatcgttt ttatcttttc ttttaaaaat gaaaaaaaat tcaattaaat
    22801 attggccaaa agatgaaaaa ccgagagaaa aattattaag agatggtgaa catactcttt
    22861 ctgatgctca acttcttgca attcttttaa gaacaggtac aaaaggagaa agtgcagttg
    22921 accttgcaat ggaaataatg aataaattta agtcattcag aaatatgagc catataaatt
    22981 taaaaaaatg ggaagaaata aaaggactgg gagaagcaaa aatctgtcag ataaaagcag
    23041 caattgaaat tggaagaaga atgcaagaac aggttctaaa agaaaaaaga ataaaaataa
    23101 aaaatccaga agacgctgta aaatttattt taccaaggat gagagacctt aaatttgaga
    23161 tttttaaggt cttacattta gatgcaaaaa atagaatttt tgaaatgtcc gaaattgaac
    23221 aagggactat aaatcaggca aatccaatca taagaaacat ttttcagaaa gcaattgaaa
    23281 atttttcctg ctgtcttatt tgcgttcata accatccatc gggtgaccca cagccaagtc
    23341 aggaagatat tgaattcacc aaccaactta aaaaaagtgg ggaaatactt ggaataaaag
    23401 ttttagacca tataattatt ggtgataact catattatag ttttgcaagt aatagacaat
    23461 caagttttaa agtcagtagt aatgaataaa ctctatataa tcccccattt gaagggaaaa
    23521 ataaaagttg aagaagaaaa caggacaatg gaagagaaaa taaaaaaatt aaaagggatt
    23581 cttaaaaatt tacaaaatgt tattgtatct tattcaggtg gggttgatag ttctttcctt
    23641 ttggaagtag cagttgaaac aattggaaaa gaaaatgtta ttgcagtaat tagcatatct
    23701 gatattttta ccaaagaaga aaaggacttt gcagaaaaat tttgtgccag ttgtggagta
    23761 agatacatta aaataaagac aaaccaactt caaaataaaa attttataaa aaatcttcct
    23821 gatagatgtt tctggtgtaa aaaagagtta tttgagaaaa tggaaaaaat aagaagaaag
    23881 aataaattta attatataat tgatggaaca aacaaaaatg acgaaaatga ttatagaccc
    23941 ggggagaaag caaaaaaaat ttttaatgtc ctttctccat taaaagaggt tgacataaca
    24001 aaagaagaaa taagaaaata ttcaaaaaaa attggattac ctacctggaa taaaccacaa
    24061 atgtcctgtc ttgcaacaag aattccttat ggagaaaaaa taacaagaga aaaattaaaa
    24121 agaattgaag aaggagaaaa atttttaatt tctcttggtt ttaaaattgt tagaattcgt
    24181 gagcatgaag aaattgcgag aatagaagta gacaaacaga aaattgaaaa actggtaaaa
    24241 atgaaagaaa aagttgtaaa aaaactaaaa acactcggtt ataaatatat aacagttgac
    24301 cttgatggat atagaagtgg aagtatgaat gaagttataa aaatatctaa ccgcgcgggt
    24361 tgaacaaaaa aaatttaaca tttttaaaag tccgcaggat cctgtagaga ttttttataa
    24421 attttatagt tgaaaatttt atggatggtt gcttcttgtt tttacaggaa aaaatagaca
    24481 ttaccaatag gcacaacgag agatagaggg gatttgataa aatcccataa gcataactta
    24541 tcgcttcggc gcttgcccgt ctgagtatta acaaaggagg gaggcaattt tcccctttaa
    24601 aaatccttaa aaatttgttt ctgagggaat aacactcttt cttggagatt atttttgtgg
    24661 aacgacggca tgactgttga caaattattt cttaaataat ataataatgt aagataatga
    24721 tataacataa ggggaaccaa aatgattaaa gaagaaaaag aagaaatggt aaaaactcaa
    24781 attattttaa catcagaaca atataaggta ttgcgaaatc ttgccaaaga aagagatgtt
    24841 tctatggctg ctcttatcag ggaagcagtg gaaaatgcct atattaaaaa agttgattat
    24901 caggaacaat tagaagcatt gaaacgcata tcttctcgaa aaggagatgt ttctgactgg
    24961 gaaacaatga aaaaagaaat tagagaagga tttataggaa aggagtaaat gaatatattt
    25021 ttagacactt gtatttttga gtatgcacaa ggcaaagcaa gtatttataa aaatccctgt
    25081 cttgatattc ttcaagcaat tttagataac aaaattactc cttatacaga cacagaagtt
    25141 tttcaagaaa tttggtatag gcattatagg agagatgaag acagaggaat tgggttatta
    25201 aaagatacct taaaaataat taaacaaaac aatatcttac caataactta tcgtgatata
    25261 actttagcaa ttgaacttgg aaaagagtat ggaaatataa ttgaacccag ggatgctttg
    25321 catttagcag tgatgcttaa aaatcatatc gacattattt gtacggtaga caaaggaata
    25381 aaagcagtca aagaaatcac cgcaatagac cctattgatt tagaagaaca atttaaaaaa
    25441 tagtcccttc ctgttgtgtc aaataaaaat ctacttaaaa taataattcg ttgagatatt
    25501 aaaaatctgt tccaacacat tgtttctttt tctatttgga taatttaagg gagtagttta
    25561 caattttaaa aagatagttg tttctgtttt tacaaataga aaaagggata ttaaaaaaag
    25621 gagggacaat ggaagagata aaattgaaag tggatgggta tacaaagttt attttgacag
    25681 taattgcagt tgctcttctt ggtctattat ttaaaccatt gttgttttct aaaaatttaa
    25741 cagcaagtca gcaagaaata gatgttaata tcactaaaat tgatggttat cgtgttagtt
    25801 cctcttttgg cactcccatt aaagtaaaag ttgttaagta ataaaaaaga aataatactt
    25861 gttttgttat aaattttata aattagaaaa aaaaatatct tgaagtattt tcatcttaaa
    25921 ttttgttttg ttataccttt ggtcctattc taaagtagtt gctctattta ttatttgttt
    25981 ttctcatatc tccaaaaatc ctgcggagaa acaatatcaa ttcctttata ttgttttaag
    26041 ataagtaaat cttcatctcc acttataata cattggcatt taccctgaat agcagtagca
    26101 aggataaaat catcttttgg atcacgagaa atattttctg ccagtttttt aggagttaca
    26161 attattaaac gactttggag taattgaact gccttattta cttcatactt agaaaaatta
    26221 aatttattaa tcaagttttc agaaaattcg gtaaggataa attttgaaat aaaaatttca
    26281 tgaaaacaaa cacaatgttc aaacaattcg ttacatactc cacgcgaaat aaaagcagcg
    26341 attaaaatat tggtatcaaa tactattttc atgagacctg ttcaaaaata tccttatcag
    26401 tatacacacg ggaacttttc ttcatcatcc ttgcacgcag agttctaaat tctcggataa
    26461 acaaatagtt ttgtaatgat tcttttatga tattactacg agttgttcct tcctcctcgg
    26521 ctattttatc aatttgtttt ttcatatctt ctggtatgct tactgttata atttttctca
    26581 tattttacct cctgtaataa aatttaatac aaaattttat ttttgtcaac aagattttgc
    26641 aggaaatgat tttaatatca ctttattaaa ctttaaaact tcaactttga aataatcttt
    26701 accaataaaa taatattcct tctttttcct tcctttgtaa aaccgactaa agttgcttac
    26761 cttgtttctc agagttggtg catcatcgtc ttccagatag cacaacgaag gttaggatgg
    26821 atttcttata aatttccttc ttaccccttt gcaaaagtag gaaagtctcc cttatgagca
    26881 ataatatctt ccttcttttt aatagattat tttgataaac aatatacatt tgtagcaata
    26941 atattcgtaa tgaaatctca taatatataa tggaattcag ttcttaaact cgctactaca
    27001 ttttgtaaat tgtttattct taacttatgt aatttttctt cctgcaaaat cttattgtca
    27061 atcaaacagc atccctcaaa aaattttgac aaagttagat aatagtatta caatgaagga
    27121 caagcaggga cataaacagg gagataaatg gtaagaaaga gattgtttat gttttctctt
    27181 gtggcacttt ttttaatagt atctaaaagt tatgctggta gtggatggga aacaacagga
    27241 aaagttttaa caggagttat aacaggagat attattaaag atattcccca ctgccatcac
    27301 aaatgttgtt gtaaagaaga atacaaagag acctatcagt catatcttaa tacataccga
    27361 gtttggattc cagcccattg ggaaaagaaa actgtccatg atattattcc tgagggatat
    27421 caaacaatta aagtatataa agaagatggt tcatattatt atgttgaaaa gtatattcca
    27481 ccaacaataa tagaaagaac aataaaaatc tgggttgaag gacactggga atataaaaca
    27541 aataggtatt gataagggaa aggggaaaaa taaatggcaa gaaaaataag aaaagacgct
    27601 tctttagctg gcttgcttaa acgtgctgca atagatccga aagttctgaa gacatcttct
    27661 ggaagaaaag taaggaaaga tgcgaaagtg aatacactca gaaagaggtc aaaataacaa
    27721 agatacaata aaaagataat aataacttct ggttgaatgg aggaaatatg ggaaatttta
    27781 tattaattgt aggaacttca ttaatcattg gaatattgtt gggagtcatc ctcaaaacaa
    27841 aactatatag acaagttcag gaaaaaaata gtgaagaaaa aacattatct tcacaagtta
    27901 aagaaagacc tattaaagaa atacctatgg aaattgtaat aaaatctggt aactatcctg
    27961 atttaccacc attggttttc aaaagaactg aatttaacaa aaatattgaa aagcaactta
    28021 ccgatatggt aaattctgca aaacaaatta tcccaaatgc agctgtttct tcctatgcta
    28081 tggcacagac agggcatttg tataaagcca ctgctaccaa cctgatgaaa tatactaatg
    28141 gcacatattc ttcaatactt aaagatttaa aagggcaaaa gattgcagga cacgcaggat
    28201 tcctaaaaat aagtcctgta gaggttttcg ctccgattat agtttttcaa gcgatgagta
    28261 tagttacagg acaacattat ctgcatgaaa tttctaagca attggaaaca attcatgaac
    28321 ttttagataa actgctacaa ttaaatctta cggaaaaagt aggcatttta ctaaatggat
    28381 atgacatact aaacgagcta tcaagtagaa aaacatatat aagagaagac cttattatga
    28441 ttcatcgtat tcgagtccaa gcgggtggaa ttaagcgtca atatcaaaca atgataaaat
    28501 cacttgatac taataatcta agaaaaacaa caagtcagct ttcaagtagt aaaattgatg
    28561 atttaaacaa tgcttttaaa aatataaatc aggacataac tcaatattcc caaatattaa
    28621 tgatggcaga gcgcatagag gttacatctc ggctgttaga atttaagata gggatggcaa
    28681 atgcacttat taaaaaagat gatatgcttc tttcaagaac gaaagaaatt ttaaaagata
    28741 tcgaagattc cctgaaatat aaaaagttcc aggaaattag agataaatta caacaaatga
    28801 agaatgtaat tgcagaaatt tcggaagaga taaaaaagaa tttttgggct gtaatacctt
    28861 cagagataga agaaaataaa agatgtttag aaaacactat acaagaattt acagagcaat
    28921 tgacagaaca tgaagatgcc ataaaaaaat ttttacagga atttcttact caaattaaca
    28981 aagaaagaga actttatatt gtcatggatg aacaaggtaa tattatttca cctgtgcaaa
    29041 tagaaaattg aaataatttc taaattttta tatcatatag gactctacga cgaaatcttt
    29101 tagttgaatt agataatttt tttaaccaaa taaaaattta gtatttctga tgaattaaaa
    29161 acaaatacta ctatatgaat tcaaaaagag aaattatacc aatttgacag catatataga
    29221 gtaaatattt tcccctcacc gagcctctcc cctcggtgga gagggaaagg gtgaggggga
    29281 atgttacttt ttattatgtt tttgatgtaa aaatagtatt aatactgata tgttagataa
    29341 aagagcagca gatgctttta agtttttaga acaaatagcg aagttttttg atagtagtca
    29401 aacgaaagaa gtcaatgata ggatattaaa tcaattatat tcagaaaaag ggtatatccc
    29461 caattccaaa atacaaataa cacctaaaat ggttgttgat attgctaaca ttttggagag
    29521 agaagtagtg gagccagatt attttgacct ttcagataaa aaaataatag aaatagtaga
    29581 acaattacaa aagttttaca aatccgatag ggttattcta aatgggaaat tttttgataa
    29641 ttttgttgaa attgttgatg ggataactta caaagaatat gaaaaagata tagaaaatca
    29701 aacttgtgat ataacacctc ttgattatta tcttaaaggc atctcatatg cagaaaaagg
    29761 ggactatgaa caagcattac aatattatct acaaggatat gaattgataa aagatgaaaa
    29821 agcaaaagaa aaattactct tttgtcagaa tattttaatt ttattgatat gtcttggaaa
    29881 ggcatcagaa atatataagt gggaagaaga atttttagga cttatagaat atgaaaaatc
    29941 agcccatgag aaggttaaat tgcatataga gataggacta tcagggaaaa ttcaaaaaaa
    30001 ctatgtaata gcaataaatc attttttcct tgcaatagat acaataaaaa aggtctttct
    30061 acacggatta tcaggtataa atatttcagg aatgccttgt atatatgaat ctgataataa
    30121 atacccaata gatgtattgg agacagtact ttacataaaa tgtatattgg agatattaaa
    30181 agatattcct gatattcctg aaatacaaga gaaaaaacaa aaattgttat cagatatgag
    30241 ttttataata ctgcgaatgg aacaaatagt tgaaaaacaa atatttgaag gttctgatac
    30301 atcaaaaatg aagaatgcac atattccttt aaatgaaaaa gaagtaaaag aaatccagaa
    30361 ccaaattcag ttatatgaaa aagatataga aaaaaatcct gatgatgttt ttgcttattt
    30421 tcaaatgggg ttgctttata atgagagaga aatgtatgaa gaggcatata attgttttga
    30481 aaaagcatta gaaataaacc cagaaaatat agagttgctg tttcagaaag gactggcgct
    30541 ggcatctttt aaaaaatatc aagaaggtct ggtctatatt aataaagcat tagaaataga
    30601 ccctaaatat atactggcat tatggggtaa aggggctacc ttagaagaaa tgaataaagg
    30661 gcaggaagca gattattatt ataatatggc tcttgaaata gagcctgaga atatttttat
    30721 tctaacagat aaaggttcaa atctgagaca attaggcagg tatcaggaag caatagacac
    30781 ttttaataag gcaattgcca tagaccctga atttgcagta tgttattatg aaaaagcatg
    30841 tgcagaaagt ttagataacc gaaaagatga agcgctgaaa agtttaaaaa aagcaataaa
    30901 attagaccct tcattaaaag aaaacgcaaa aaatgatgca gattttaaaa atatatgtga
    30961 agataggact tttaaaagaa ttgtgagata gggagtcctc atgctaattc tctggttccc
    31021 gttcatatcc gcaggatcct gtgaatttaa gtaagagagt agaaagtaat cctgtccaat
    31081 caacttatat cttctgcgtt tgtctgtcta agcatatagc gaaggaggat aaggatggta
    31141 cgtaagtcca ctctaaacat ctccacagga ttccgtcatt cataaggcga gcctctgggt
    31201 agtttatagg acgaactttt ggactgtata gaacaattcc ctgtcttcta aatccccctc
    31261 tttatccccc tttacaaaag ggggaaaata ttctcccttc tttgacaaag gagggagggt
    31321 aagggtggat ttttattctt ctgctaccct gcaatcctca aagttctgca taatgatttt
    31381 cctcgagatc ctttgtaaaa gaccatgttc tgatattatc tgagtgttta taaaaactaa
    31441 gtatgatttc tccttcctat tctacttttt aatactgttc cagtaataat taaaactgcg
    31501 aaagagataa taacttttgt agtttgtctc ataggtgata aatctctacc aaagcaggtt
    31561 gtagcaaaat gttcgcaatt tttcgttaat aagttgtatt taccattata ttcgtctgga
    31621 tttttgtaaa aattcttggc tcgttctatt actttctggg cgtgtttttt attttttggt
    31681 tcagtatgta taacaacatg ctctttttct gcaaatttct tcaacgattc catacgaatt
    31741 gtagcaccat ttttcccaaa aataccttta tctacaccat gtcctaattc tggattatgg
    31801 aaatggatta cttgattatc tcctatgtat attcccatat gccaaaaaat acctgatatt
    31861 cgtcttttta taatactgcc tggttttatt tcgtttggtc ttatctgcat ttttccccac
    31921 ttatataaaa tacttttgaa attttgtaaa tatttactca accctgcctt tctgataaga
    31981 atatttattc agttttatac tatattaaaa gcaaagttaa agttcaaatt tttttatcaa
    32041 ataaactcgc tcagaaaata aaaactctct taattctgaa aatacattaa aataacttct
    32101 tcaagtttag ttagatatat tattgagcaa taaaatatct tagatgctaa aaattaggtc
    32161 tactctaatg gggttcaatt aggatttgca tttctgtaaa agatttttct atctcaccag
    32221 gtaaaaaata tcctttctta ctatattttt cttctaattg tttttttatt tcttctgctt
    32281 tctctactcc aagaattgag atttttttag ggtagcccag tttccacctt caattgccca
    32341 atcccctagt ataactccac ctgaatttct tctgcagtcc gatccgtttt cttcaattca
    32401 ccattgaaaa cattaggtag ttgctccaga aactgcttct tccatttcgc tactcggctc
    32461 ggatgcacct gataatggct gactatttct gccagggcct tctccccctt gattgactct
    32521 gtcgcaacgc gctgccttca gcacccctgg gactacaaat attttaccta aaactcgctg
    32581 ttttctatgg ctgatcaagt tcctttactt ccaccttatc tatccagaga gttcctgctt
    32641 taccctccgt aataatgtat acccttgctc tatcctgttc agggtgcatt cgagtttgaa
    32701 aagtatattc tttccactcc gggccaacat taacaatttt attcgtagga ttggggtgtt
    32761 ttctccaatt gtagtcaaga acttttaatc tcacaggtaa atcagagatg tcgcttttta
    32821 agtgcactga aagaacataa tctctcccgc cgtttacagg tggcgctcct tcttcggtcc
    32881 aaacagaaat attaggaaat ttaggagcta ttaccttttt atatcctttt tttgtctggg
    32941 ctgtttcggg agtaaaaata aactttattt cttctttttc ttcatctttc ggtattattc
    33001 tcaatccaaa aataccgtct gaaatggttt gtaaatctaa acttccatct tcaattttta
    33061 ctccaaaagt atactcttta attctgtttt cgtactcagg gaaaagctct ttgatctccg
    33121 gaatatcttt ttcttcaacc tttaagggat gttttgctcc gttctccagt tcagaaatgt
    33181 cattaggact tctcatggtt gcaatcacag tatattctcc attgtccaga gaaaccttaa
    33241 aaatgccaga gaacctgtgt ccatatctta ataattcgtt tttactaccc agatcaaagg
    33301 aactcgcctc ttttgtccat ccataaccgg tatcaggaga aaaaatagtt gtttttgaaa
    33361 caaacatcct taaacttttt ttaccttcca agtgttcgtt ggagtccaaa gaacaatatt
    33421 ctggatactc ccaacaaagg ttgcttgccc aataaaccgg ttgaccagag tcctcttctt
    33481 caaaacccgg gttttttatt ccaggtaatc ggtcaagatt ttttgtccat gttttttctt
    33541 cctttgttgg tactttctct aaagagagca gctcaatatt tacctcaatt ggcttatcag
    33601 gtaattcgga caatacacct atttcgtaaa cgtgagtggc atagggttca aaattatctt
    33661 tgaaggcgcc gtttactata gaaactgtcc ttttttcaaa aagaactttc ccctgcgtat
    33721 ttgtatcaat attagataat tttatttgta catttttttt ggtatcttca cttatgttgg
    33781 cagtaataat atacgctttt tcattgtgta actttaagag taaatcaatg cctgtttttt
    33841 cttttccgga tacggcttga ctagggctac tggtgagtaa aatcggcgtt aattctttta
    33901 tttctccggc caatttcttc aaggtctcct tcatatcctg cgaggcgata ttatactccc
    33961 agtaaacaat ccctcttgct tcgtgaataa gatccagata tgtctgacat ctttgttgag
    34021 aggatgttat tttactttct tgactccacc catctgcctg cggtgttacc caaaatggtt
    34081 ttctgatggt attcgctact ttccaataag tatctactct ttccacgtgg cgggttattt
    34141 tttctccaag tcgcggttgc caatagggat cagtatccaa tatgtcatag cgatcggatg
    34201 gtatcggtgg accagaaggt tcccagaagc atgggaaaac gagatgatac ggatcatatt
    34261 ctttagttat cctgtactcc acggaaacac cggcggcttc tttttctccg ggttcgtccc
    34321 agaaatatcc aaaaaaggca ggatggtttt tacacatttt tattttagga attaaagaac
    34381 cttctctgat aattctctct aattcgtcaa gaggttttgt gctgaaaaaa tcacggggag
    34441 cactgtgctg gtaggtagaa acaattacca tcattccatt atagtaggcg gtatccagaa
    34501 attctgggga ttcaggccca acagtaatat taaatccggc ccgggcatat tctgaaattt
    34561 tatcctgagg aatatccata attcctattg caaaaaaagg ggtattgttc actaatgtcc
    34621 agagattttc tcgatctgac tttatttcat aaatactcgg agtatattta attaatggaa
    34681 tggcactcaa tgagataaca gattctttct ggtatacaga gagaagagcc atgtattccc
    34741 caggtggtaa attgtcaatg tttatctcca cagaaaaatc tgcctctgaa atcgggatgt
    34801 tcgtagtctc ggtaatcgtt ttgtctcctt ttaccacttt cagagatgcg gtaaggccaa
    34861 aggggagtat tccttctatg ttaattttgt ttttaagttt ggcatttttc tcatcagtat
    34921 aatagtccct ggtaaaatag gaactaacga gggaaggcat ttttacctcc tttgctgaga
    34981 gatgacaacg cttgttagat ataatatcaa aaatatctaa ttggaattta tactttgtat
    35041 ctttctcatt aagggaaatt gggaaattta attctttatc ttcattggcc aatagagata
    35101 ctgtttcttt aaattcctgt tttgtcgtaa gacatttcat tctgacatca atctttcttg
    35161 attcatggga acaatttttt aatttctggg agaaaactaa acctggccct gtctgtccca
    35221 atttgtaggt caaacttgaa gatgatgggt taatttggta cagcggaaaa tcaatgttta
    35281 tatttttaac agtagcgaac atctctggct tgtggaatcc cccgaacgta ggtgcccatg
    35341 cactatactc tggttctacc ttacgctccc gacaaagatt tatcccccag gtgctggatg
    35401 cttcttggct gaaagaaaaa ttgtaaaagg gaatggcaat ttccgccgac cagaaatcct
    35461 ggccttttac tgttttcccg tcccagaaac catcccaacc agtttcctca caaaggggat
    35521 ctatccactt ctcatctgcc tgagtattta gggtatttaa actaaaatga tagtaacttt
    35581 ttctatctaa attcgtatca atgaatacct ctacgcaatc atcttcccat acctttccgt
    35641 cccggtactt ttcatctgct ttaatcttgt ctatataggg ttccttacat aggaatccaa
    35701 aatagatgtt attgtcgtcg taacagatgt aacaagtagt cggacaccgc acaggtccac
    35761 cagtcatatt ttctgttagg tcaacttttc gggcttgttg ccaacaaaga tcattaagat
    35821 taccatctat ttgggggggg acgtctattt tgggtataga aaccgactga ggagtatttt
    35881 cccctccgct tttttgatac tcttcttcta cttcctggtc agttagtgca cctctatata
    35941 tctttacctc atcaataatt cccttaaaat acctcccatc tttataaact gatccgataa
    36001 atacactttg gtcattagta tagggtagcc tggtttcctc atagacggaa tccagtcttc
    36061 cattgatgta aaacttaact ttataattat catatgttac taccacgtga gaccactggt
    36121 tgagttttat gaccgagtta ttattctctc cgtaagtgaa ttttcctacc ttaccacctt
    36181 cgccaaaagg gccataaaca gagaaaacga gttttttgtg agcgcccaaa tatagcccgt
    36241 acacggaaca ggtataataa cctttaccaa caattcccgc ccaatagacc ggagtacttt
    36301 caggatatgc cttagggtat atccacgcag aaagagtcat actggtaaga tcaaaacaac
    36361 tgttagcaga gtcctttacc tccacataac taccttttcc gtcaaattct aatgcccctt
    36421 taattttacc ctcagtccat accgcgttat gaatctcacc aggatgtcca tcagcagaat
    36481 cccaggctgt ttctccactt ccttcatcaa atttccagta tccgactaac tccgctgaat
    36541 aactgctgct aactaaaaaa caacacaaaa aaatcaacca tatacttctc ttcattttcc
    36601 ctccttgggg agtcgcttgg ttgttttgta accttatcac ccggctgtcg cccatcttcc
    36661 ctattattcg ctggtaaccc gaacataaaa acatcctctg ctatttaaaa tcagcagaaa
    36721 aataaattat ctctaactct agtccggaag attctttatc ttcgtcagag acaatttttg
    36781 ttccaggtga ccggtaatat ctcacggagt agatatatca agccaaaagt cgtggtcgtc
    36841 cctcttactt tcaattttcg cttttgagtt ccactccaca tgtccgtcta cataaaggat
    36901 atttgctccg ttgttgtgcc tgtagtgaaa ataaggtgtt tctgcatcga acattacgta
    36961 tgagataaag aggaactggt aattgttact cttattgtaa gtactattag catctgcaat
    37021 aaggaatgct ctttctggtt ttctcacccg gtttaatttt ttgggacaat accgagaatt
    37081 ggaaggatac tggtaaacat caccataccg tcctacccag ctattgtatc catagttagg
    37141 accccctgag acgcctccca cagaaatcac ccgttctttt tctcccgaag gacagatgaa
    37201 caactttttt atgttacgag gaaccttagc agtccacccg ctccatactt cataaccttg
    37261 tccggctggc gctcccaaat aaggcatcaa ttcaccaacc catgtgcagc tccatacaaa
    37321 atagccaccc gagttcactg ccagcggtaa ccaatcatca tagtcatttg catacatttg
    37381 aacagccagt cctaattgtt ttagattatt catgcaactt gtctgtcttg ctttttccct
    37441 tgcctgtgat aatgcaggta ataacattgc ggcaagaatc gctataatcg ctattaccac
    37501 aagcaactct atcaatgtaa accccgtatg ctctaactta g
//
LOCUS       DAOOVW010000013        37435 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_714_length_37435_cov_8.520760, whole genome shotgun sequence.
ACCESSION   DAOOVW010000013 DAOOVW010000000
VERSION     DAOOVW010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 37435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37435
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_714_length_37435_cov_8.520760"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..1292)
                     /locus_tag="PLW95_02995"
     CDS             complement(39..1292)
                     /locus_tag="PLW95_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOV21632.1"
                     /translation="MGKTIIEKIISNHSGKNVKSGDISIVEYDLLMSQDGTTPLVINS
                     FEQFSEKKIPFPDKCVFVLDHNSPSPSQGVSALHKLMRDFAKEYNIRIFEVGEGVCHV
                     LLPETGLVKCGSLVFGADSHTPTYGALNSAGIGVGSTDLAIALYTGYLWLRVPETIKI
                     VFNGNLQDGVFAKDIALFMVRQLTARGAIYKSVEIKGETIENLSMDGRFTICNLTTEI
                     GAKCGIMEFDDKTKEFLKERKIENYSPVFPDNDANYEKIYNFDVSELPPQVAKPHTVD
                     NVSDINEVEGIEIHQGFIGTCTNGRTSDFEIASKILKGKKVKKGVRLICTPGSRKIYL
                     ECLKNGYIDALLEAGAIITNPGCGPCVGTHQGVPSDNENVISTANRNFKGRMGNQNAF
                     IYLSSPATVAASVIEGKITDPRKYL"
     gene            complement(1296..2306)
                     /locus_tag="PLW95_03000"
     CDS             complement(1296..2306)
                     /locus_tag="PLW95_03000"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HOV21633.1"
                     /translation="MQRNSNTFNYFMKVVDNISPKLSWNAKTISTHYIWRKKLESKLQ
                     ELLGKMPKKVPLEVDWDEKIETKSFIRNKIYIRSEKDYWIPSYYFLPKKLKAKTPAII
                     CLHGHRGILPYIREGTKEQKALGKRHSLDYAPFFAENGYITIAPIQRGWNETRYAEPS
                     QYGDCYRVTMDAFLIGMTPIGVRVWDCMRIVDFLKTQDEVDKLKIGVAGLSGGGTTSL
                     FLSAMDERIKLAMIAGYFCTFRDSIYQIFHCICNCVPHIMEWAEMSDIAALIAPRPLL
                     IISGEKDPIFPIEGTKKAYKMLEKVYKMFGEEENLENDFFEGEHQWSNRKTIQFLKKH
                     FI"
     gene            complement(2316..3548)
                     /locus_tag="PLW95_03005"
     CDS             complement(2316..3548)
                     /locus_tag="PLW95_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HOV21634.1"
                     /translation="MNKIYIIDVTNRDGVQTARLGLAKLEKTMINYYLNQMGIFQSEF
                     GFPLTSHEINYLNANLKLVKKKCLYPIRLSGWLRAIPSDVENAFKNVPDLQHLNISIS
                     TSDQMIVGKFQGKMKFKDVIKNMGDAVKSAKEKGAKTIGVNAEDASRTDLKNLIEFAS
                     AVKEVGANRIRYCDTLGYDSPFSIYERIYKIAKEVKIPIELHCHNDIGMAVACSVAGA
                     KGAIDGGVDAYINTTVNGLGERTGNADLVSVILAIKYASGIEGKYKLDEKVDLKMAWK
                     ISHYASYAFRVPIPINQVGVGENAFAHSSGIHVDGALKDRRNYELYNFEELGRGEPEI
                     IETGRMIILGEYSGIKGLRNVYGKLEIKFKDEEEAREILELARYANVHKQKPLVSDEL
                     IFIAKYPEEVKQILTMTP"
     gene            complement(3561..3992)
                     /gene="rplM"
                     /locus_tag="PLW95_03010"
     CDS             complement(3561..3992)
                     /gene="rplM"
                     /locus_tag="PLW95_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOV21635.1"
                     /translation="MEKTSVAKKEKIKRNWYVVDAEGKILGRLASKVAGILMGKNKPI
                     YTPNVDTGDFVIVINADKIKVTGKKLVDKIYYKHSGYLGGLKKENLFSLLNRKPEFVI
                     KHAVWGMLPKNKLGRQMIKKLKVYRGTNHHHEAQKPEVIEF"
     gene            complement(4078..4311)
                     /locus_tag="PLW95_03015"
     CDS             complement(4078..4311)
                     /locus_tag="PLW95_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21636.1"
                     /translation="MKENNNIDFNSNEWKEKLKGKSPEEIAKIVGDYYEDKYKDIPEF
                     DKKKIIIWIVFLIILSVIIIFIFSYLKLLLNNI"
     gene            4381..5526
                     /locus_tag="PLW95_03020"
     CDS             4381..5526
                     /locus_tag="PLW95_03020"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOV21637.1"
                     /translation="MNKVRIGVIGVGGMGSAHCLIIKEIEEGELTCVSDVEEEVAKKK
                     GEEFGVKSFTDYRELIKSGLCDAVIVATPHWFHPEISVFAFENGLHVLSEKPIAVTVS
                     AADRMIESAEKNNKIFAVMYQRRAEWLIRKAKEIVTNGKIGEIIKAVCIDPWYRSQAY
                     YNSATWRATWIGEGGGVLINQAPHTIDIFTYLAGLPSKVEAKTRTKLHDIEVEDEVSA
                     FLEYRNGAWGYYYTSTCEPVGPFYLEVSGDKGKIVIRGDQITFYNYETPIKEFTEKAP
                     DMWASIKTEEEKIIASSNFETGHKVIIKNFVRNILYNEKLIAPGKEGLNTIEFMNACI
                     LSGKKNKVVNIPVDRKEYDDLIETLKKTSRGKKVVKIQRVTDPNIKK"
     gene            5625..6821
                     /locus_tag="PLW95_03025"
     CDS             5625..6821
                     /locus_tag="PLW95_03025"
                     /inference="COORDINATES: protein motif:HMM:NF039309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOV21638.1"
                     /translation="MRGKILSAIFLFFLVLSPFFLIKYRNSTKKEKEEKYRIIDGVIF
                     TGDCIINILQQSGISSNVALSIANKLSTVFNTRSSNIGDRYRIYFDNENGNFIKFVYY
                     SVPLDFYTVQYDEKNKDFFVSSGKVKAEKEILGVKGEINSSLYDSMIKQNVNSEIIIQ
                     FAEIFASKIDFFTDCKKGDKFVILWESYKDKKGNILKDIKVVAGEYINYNNTYYAFYF
                     EPQDNKKGGYYDENGQSVETAFLKSPLNYRRISSYFSYRRFHPIHKIYQPHLGIDYVA
                     PAGTPVSSIGEGVIIFAGFSNNGYGNNVKIKHPNGYISYYGHLSKIKKGIKKGISVKK
                     GEVIGFVGMTGLATGHHLDYRIKKNDKFINFLKLKLPPSYPIDKKYIPEFEKIKEQYI
                     EKMSTF"
     gene            6853..7278
                     /locus_tag="PLW95_03030"
     CDS             6853..7278
                     /locus_tag="PLW95_03030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOV21639.1"
                     /translation="MKKGFSFLEIVIVIFIFSILLIPTTFVILNYQKNLSLESAVSEI
                     VEVLDYARNCAKSEREKFLVKFEDKNFYIIKNGKVVNKIQHLPQHIYFKEITDGLNPV
                     VFLPDGTTEMAGHIILGIENKNDEKKIVVNNITGKCIIK"
     gene            7275..7637
                     /locus_tag="PLW95_03035"
     CDS             7275..7637
                     /locus_tag="PLW95_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21640.1"
                     /translation="MKKGITLINILVSLIILSIGIITLLKVYPVISSLSERGKDNATI
                     SMISTQIFTLIDKVYSSSNNDLPSSVDGCFLEFPQYKYKIDFSEERKNLYNADIEISW
                     KRKGKIEKRYFTYSFRRE"
     gene            7597..8226
                     /locus_tag="PLW95_03040"
     CDS             7597..8226
                     /locus_tag="PLW95_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016508513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOV21641.1"
                     /translation="MKKDILHIHSEENKGFTLIEVLVSIVILTIIIFSSMFIFKESVR
                     KFRKNTYEKEIYKTATETFQTIEKYLKNAKINNLNGKLRMNFKGQKQWVKFISTTEES
                     KESDFLKVGIYFQDNNLKMSIEKIDEESDFSFKKGFPGAQILAENIRNFEILYFDGTN
                     YVSNWDTENTEEPQLPPFVKIIMEIESGMKVEGKKLNKNFERIIKVGYE"
     gene            8219..10492
                     /locus_tag="PLW95_03045"
     CDS             8219..10492
                     /locus_tag="PLW95_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21642.1"
                     /translation="MNRGVSIIFTIFILFLLFIFTATMLFIFQFWEKTSFKFFSSRQA
                     YTFAKNGIEEGIWEINNDDKKYDCLNDKWRVNFQGKEVDLNEDGKFDSKWFYIKNKKG
                     EIIGRYAVLVEDESGKVNINSSGNLSLNYNEGYGCHEISLFREIIGEKNMINILHFKY
                     GNDFFPGTRNFDDNKNQNSYETDGIDNDADGIIDEYGEGIDEPEEFNFEKPYGDDRPF
                     FDISDIKLVDGIGQDVFNRFKNYITVFSYDLERNKNDEERININTADFNTLFSFFQSL
                     GYSEKEACQISLNIIDYRDNDSIPTIKKLGNSLFIGIEDTPYLNEIDAVKEWENKTLE
                     SGAIILTEKGGQFVEIFNPYDREIDIGGWEVKGVLTLFSNLWNGIFSTSEQVLDEVEN
                     GETEIVQPDFLGNLVPTTIKIREGVKIEPHSFYTIGDLITIVIIMVPPDFVPIILFLP
                     THDPSDCSQYEPIIAINPGSFGAISKILSSIPFLSKLGLDFTVSLYDKGGNLIEKTDY
                     PLDTPWTTVQKNDPRMSGSGNWFPSISTPNTFNSVFMPFVGEEFNSVNWILNWGSSFA
                     IKNNKFSSVGEISLIHKNAQWKTINLWKNISDRKIIDNIKVRKEEKVYGKININTSSE
                     DTLKCLPLVSKITAKIICAGRPYTDITEILGSPSGMNFEKVLLSRDITKYGFDGIDND
                     RDIYIDTEKEKEMVFSKIVGLITVRSNIFKITSIGEKVQDKNNNGKIEDEEIIATKKI
                     NAWYDRRKKKIIHRKEI"
     gene            10492..11415
                     /locus_tag="PLW95_03050"
     CDS             10492..11415
                     /locus_tag="PLW95_03050"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOV21643.1"
                     /translation="MGKLKIESGKFTIVKLKNFSKKHEKKIILFFLCILLVLFALLFL
                     HFKKIKDETVAVVNGYRITFDDILTKAETSPVFYQEYLKIEPKFFVEEYINQILLFQK
                     AKQNERKYKKEIEEKMKNYYIDLLTNIYVEKELTEKIKVSEEEIQDYYNKNISDFVIP
                     EKVNLYEIVTDSKDKAVEILSRLSIGESFEDIAERESISNSAKNGGNLGWIDVEKLDH
                     EIASLVTKISPGQILANIFKTDMGYHIIKLAGRTERRVLTLEEARQSIINMIKTQRKK
                     KEVEKFVKNLRENSKIQIFPGKIEEIKTRIK"
     gene            11412..12203
                     /locus_tag="PLW95_03055"
     CDS             11412..12203
                     /locus_tag="PLW95_03055"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOV21644.1"
                     /translation="MKKIIFIFAFFVPFLFSADKIVAFVENSPVFESEVYQIISYDKL
                     SYDEALEKVIQRKLIVEKSKKEDIKVSDEEIGKEFQKIRQSFPDEKSFMENLKKNGLT
                     ISQLKNIIKEQIISRKYIDKEIISKIEIPPSEIANFINNHLEITKYNFKFKWFETKKA
                     AEEFCLSPDITKMEESGYLKEDEILSEILTNLKKIGKGEFSSPFEVGNKFIVVYLSDV
                     KKEKVENLKDLYITARRKIFNEKFNTMYKQLVSKLKNEMLVRIIE"
     gene            12200..13174
                     /gene="pdxA"
                     /locus_tag="PLW95_03060"
     CDS             12200..13174
                     /gene="pdxA"
                     /locus_tag="PLW95_03060"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOV21645.1"
                     /translation="MKITGITLGDPAGIGGEIFLKGYNEIKKIKNSFPVLIGDRIVLE
                     RNATSLSQKISIKEIKNKNQIDKNCINIFPVNYIKEKNYPIGKNSKICGSASFKYIEF
                     AIDLWKKNQIDALVTLPISKKAWEKAGIKYPGHTELLSEKFNARKTAMIMVADKIMTV
                     LATTHISLKEAIGKLSVQLIVEKVKNGIEFLEKIKVENPVIGVSGLNPHAGEEGLMGD
                     EEIRIIKPAIKKLRKQGINCIGPIVPDAIFRKNIEGEISLIISMYHDQALIPLKTFYF
                     EKLVNFTSGIPMIRTSPGHGTAFDIAYKNKGNPSSFIESYKLAVELLK"
     gene            13175..14035
                     /gene="rsmA"
                     /locus_tag="PLW95_03065"
     CDS             13175..14035
                     /gene="rsmA"
                     /locus_tag="PLW95_03065"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOV21646.1"
                     /translation="MDKFLTKKKLRELLVSENLDIKKYLGQHFLVDRNARDKLLSFAE
                     ISKEDVIFEIGPGLGALTEYLIEKSKCVYGFEIDNHLCKILNKRFGHYKNFNLIETDF
                     LQTDENFWRNFERVKVIGNTPYYLSSPIVFHILKFLKKISLSLLTVQKEVGEKFVAHP
                     GNKNYGPISILLSLYTDSKICYSLKKDVFFPKPEVQSVAVKIIPLEQPKIEVEKKFWE
                     FLPLIFNNRRKKLVNVINKIFKLSKEDIIKIINKIGISENSRVEELEPEKIYQLYQMV
                     NIQATVFPEK"
     gene            14127..14306
                     /locus_tag="PLW95_03070"
     CDS             14127..14306
                     /locus_tag="PLW95_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21647.1"
                     /translation="MGDKTYLNRIICGDTLSILKNMPDEIVDVGVTSPPYNKGENKKG
                     WLVSDLFYYMKNEKI"
     gene            14364..15521
                     /locus_tag="PLW95_03075"
     CDS             14364..15521
                     /locus_tag="PLW95_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HOV21648.1"
                     /translation="MAMWIGINRKARVCYGFDDVSLVPGDVTLNPEEVDISTKIGNVE
                     LKIPVLAAAMDGVVDPKFAVEFGKLGGIAVLNLVGIYTRYEDPYEIIEKIISSPYEES
                     TKIIQNIYKEPIKENLIGERIEQIKKGNVVCAVSSIPQLAEKYIGIAIESGVDIFVVQ
                     STVTTAKHISSSYKPLDLYKFCKGAKVPIIIGNCVTFDVAYQLMETGADGLLVGIGPG
                     AACTTREVLGIGVPQVTATSDCAAARDKYFKKTGKYVSVITDGGMVTSGDICKAFGVG
                     ADAVMIGSAFVKSVESPGKGYHWGMATSDGNLPRGTRVKIGTSGTLKEILFGPAKVDD
                     GSQNLMGALKLSMASVGAKNINEMQMTDIAIAPSMKTEGKIYQAARKEARK"
     gene            16085..19507
                     /locus_tag="PLW95_03080"
     CDS             16085..19507
                     /locus_tag="PLW95_03080"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOV21649.1"
                     /translation="MSKFVHLHLHSEYSLLDGMCKIEEVIPLAKKNKMTSLAITDHGS
                     MAGVVKFYKVALDNGIKPIIGCEMYIAPESMTDKTKRERNNYHLTLLASDNNGYKNLM
                     ALSTFSYLKGFYYKPRIDKEILSKYSKGIIAMSGCLQGEIPYFLLKDEIEKAEEVVNE
                     YIEIFGKENFYLEIMDNNLPEQKKVNSDILQLAKKSGIKVVATNDCHYLKREDSFSHD
                     VLLCIQTKTHIEDTKRLKFKTDEFYFKTSEEMERSFKDIPEVLENTLEISEKCNVEID
                     FNKYNLPRFYPPENKTPDIYIKEMVKNGLEEKFNLKNVNFSEKNEIVERAKYELDVIK
                     KMGFSSYFLIISDIVNYAKKNGISVGPGRGSAAGSLISYLLGITDINPLEYNLIFERF
                     LNPERISMPDIDIDFCDRRREEIIKYIREKYGENNVSQIGTYGTMAARAVVRDVGRAL
                     GMPYNEVDRIAKLINSSPGTLLKEEVVLNPEVKRAIESDENVKNLFEICLKLEGLARH
                     SSIHAAGLVITENEVYNYSPLFIGPKGEVATQYEMDWIEKIGLLKVDILGLKTLSVIE
                     DTIELVKKRKGIEITSFPLDDKKTYKLLSNGETAGVFQLESKGMQSLLRDMNVENFED
                     IIAVLALYRPGPMKSGMVKEYIERKKDSSKIKYDHPLLETILKPTYGVILYQEQVMQI
                     ANKLANFTMGEADHLRKAMGKKISSEMEKFREKFILGAKKNGVKEEIAEKIFAQISKF
                     AGYGFNRSHSACYSFIAYQTAYLKSNFPLEFITSLLNSEIGNSDKIEEYIKECERYDI
                     WVLPPDICESDGEFKIFGNDIIFGLSAVKNVGSGAIKSIIESRKEGDFKSLFDFCERV
                     NLRVVNRKVIESLIKGGAFDYLEIPRSHLFAMIDDAIEYGTRVQKSQEKGEIEIFSTP
                     VTINYTNKKLISSLPEWSELKRLSYEKDMLGVYLTGHPVEKYLPLLKVYSSIPISDLP
                     KVNNGEVVIAGILQELKRNITKKGERMASGELEGIDGKIEVIFYPETFKKYSSVIRTN
                     NIVFIKGKVQKREDDIKFIANEVIDLAEAKEKFTGTIEIHICLPVEDKKIKELKEFIQ
                     NNKGSCSVILTLYKEDNRKVKIKANGYGINPEPDILLNLKNSFPDFKINFGR"
     gene            19693..20697
                     /gene="mtnA"
                     /locus_tag="PLW95_03085"
     CDS             19693..20697
                     /gene="mtnA"
                     /locus_tag="PLW95_03085"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOV21650.1"
                     /translation="MEPIIWKEEHLYIIDQRKLPLKEEIIEIKNIKDVWESINKLKIR
                     GAPAIGCFAGFGIALSSLKCKEKTKDKFIKKIKKDIEYLKTSRPTAYNLFYALERIEK
                     VLDENKTIDEIKKDIVDEAKQIYEEDIECCYKIGNYGSSLIKNGMNILTHCNAGGLAT
                     SGYGTALSPLFVSKERGKKFHVFVDETRPVFQGARLTTWELEKSKIPYTLICDNMAAF
                     LMKQGKIDIVIVGADRITLNGDTANKIGTYNLSVLAKHHKVKFYIAAPFSTFDFSLKT
                     GKDIPIEERDPDEIRKIKNIYIAPKNCPVYNPSFDITPGEFVDGFITEKGIIHPPFNK
                     "
     gene            21063..21446
                     /locus_tag="PLW95_03090"
     CDS             21063..21446
                     /locus_tag="PLW95_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21651.1"
                     /translation="MCNIIQNNVEISERIRNVIDGIITKDIITLKKFKSETISKINNL
                     LYLLANSDVISYEKLLHTLRIDNIRTLSSLLDVLVMAGVLIRVKCYGQTYGTTRKTPK
                     YLFITPSLRNAILNGSFTSGLEGKK"
     gene            21548..21727
                     /locus_tag="PLW95_03095"
     CDS             21548..21727
                     /locus_tag="PLW95_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21652.1"
                     /translation="MKNRSNIVIEVGFNKEETRQVEKTMKKVKSSYGLVFGSDKLELV
                     NKSIVKIPLKFLMLI"
     gene            21757..21829
                     /locus_tag="PLW95_03100"
     tRNA            21757..21829
                     /locus_tag="PLW95_03100"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21790..21792,aa:Val,seq:cac)
     gene            22203..23174
                     /locus_tag="PLW95_03105"
     CDS             22203..23174
                     /locus_tag="PLW95_03105"
                     /inference="COORDINATES: protein motif:HMM:NF025022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOV21653.1"
                     /translation="MGANIIRVIFLILSILLSYCFFPSRKVIGIIIGLLGGGLIIFIE
                     ILLERISFKKLLLAVGGLIVGLITAILLTRFFLLIPIENPQTENYLRFILYFIFSYLG
                     IMLGIKGVEELGFVFPFLHSVKGDEKVAVVDTSILIDGRVYELAKSGFIDYTLVIPQF
                     VLKELHELSDCSSDMKRQRGRRGLETINKMRKDASLDVKIYDTDYPDIPSVDAKLVKL
                     AADLRAAVLTNDFNLSKLASVQNIKILNFNTLADILKPRLASGERISLKIIKEGKEAD
                     QGIGYLEDGTMIVVEKASKYVGKVADIIIDSAIQTSSGRIIFAKLKT"
     gene            23174..23848
                     /gene="ispD"
                     /locus_tag="PLW95_03110"
     CDS             23174..23848
                     /gene="ispD"
                     /locus_tag="PLW95_03110"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOV21654.1"
                     /translation="MSIAIIVAGGKGERTGKNIPKAFLEISGKPLLFYSIEKFQSFSS
                     LIIVLPKSHLEMWKDKLKLKYPDINMEIVPGGENRQASVWNGIERIKEDEEIVLIHDV
                     ARPLLSKNLIKRVLTGTEKYGACVPVIKCVDTLKKIKNKKIEKTLDRNEVFLVQTPQG
                     FKAGIIKQAYEKAKKENFIGTDDSMLVERIGLPVYCIEGERTNLKITYEEDIGIVESI
                     LNGVLK"
     gene            complement(23885..24313)
                     /locus_tag="PLW95_03115"
     CDS             complement(23885..24313)
                     /locus_tag="PLW95_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOV21655.1"
                     /translation="MITQLSIFLENRLGRLFEVVSLLGENNINIRALSLADKANYGIL
                     RIIVNKRQQANIILKGNNISVKETKVIAAKVKDEPGGLSHILKIFVDNGINVEYMYAF
                     VEKSEERAIVVFRVENPENGEKILEKNNIKVLNEDEIEKF"
     gene            complement(24323..25627)
                     /locus_tag="PLW95_03120"
     CDS             complement(24323..25627)
                     /locus_tag="PLW95_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOV21656.1"
                     /translation="MAYFNKGIETISESKLKQIQLDRLKKILDYVNKKVPFYNNLFEK
                     NKINIHKLSSLDDLKNIPFTVKKDLRENYPFGLFAVDLSEVYEIHASSGTTGKLTVVG
                     YTKNDIKLWSEVMARSLVCAGVGKEDIIQNAFGYGLFTGGLGIHYGALKIGASVIPTS
                     AGGTKRQLMIMQDFKSTVLTGTPSYALYMAEEAKSIGIETSKIPIRIGVFGAEPWSER
                     MRKEIEKIWGITALDIYGLSEIIGPGIAQECEGKCGLHIWSDYFLPEIIDINTENTLE
                     EGKEGELVITTLTKEALPLIRYRTGDIVSINYQKCSACGRTFPRISKIKGRIDDMLII
                     KGVNVFPSQVETVLLNIPEVAPHYQLVVTREKYLDKLQINVEITEETFSDEVKKLEEL
                     EKKVKKEIESVIGIDVEVNLVEPKSIERSTGKASRIIDLRKI"
     gene            25755..27530
                     /gene="iorA"
                     /locus_tag="PLW95_03125"
     CDS             25755..27530
                     /gene="iorA"
                     /locus_tag="PLW95_03125"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HOV21657.1"
                     /translation="MEKKYLLGNEALAYGLFEGGLEASFAYPGTPSSEITEKLVEISK
                     EENIYIEWSINEKVSYENAYGVSLTGRRSCVIMKHVGLNVASDPFITSVYTGIIGGLV
                     IIVADDPFAYSSQNEQDSRRYWQLAKTVCFEPSSIQESKNIAKFSFEFSEKIGLPVMI
                     RSVTKLSHGKSDVLIGKRQKKISDYGFKKNPSKFVMVPSNARVALKNLNEKQEIIKKE
                     VEKLPFSKFEKGESNIGIVACGLTYSYVKEFVETEKKNSPIFKVSTYPLPEKKLKNFL
                     ESVNEIVVFEEGDPVAEEQIYIYSKIYNLKNKIYGKINNFTDKEGELSVDKVEETFYK
                     IRGKKKKYYSFSIPERPPVLCAGCPHLGSFYILKKVFGKDALFPGDIGCYTLGVKLET
                     IDTCLCMGAGISMGSGMSRFEKNNVVSIIGDSTFFHTGIPALINAVYNQANQVICILD
                     NRTTAMTGHQPHPGTGINAKKEKTVEIELKKLISNCGVEDVIEVDPYKIKEAIEKIKG
                     IKDKKGVKVIIFKRECIITSKGKKIQYYIDSKRCKGCKLCLTINCLAMSFKNGKAEIG
                     ILCNGCGMCENICPYKAIVKDEKKG"
     gene            27514..28083
                     /locus_tag="PLW95_03130"
     CDS             27514..28083
                     /locus_tag="PLW95_03130"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOV21658.1"
                     /translation="MKKRVDILICGVGGQGILLTSNIIGIAGVEEGFSVRGTEIHGMS
                     QRGGSVEAHIRLNCNYGPQIPYGKGDILIGFEPLEVARYSNYLKENGICVVNSYKIPI
                     IGYEYDEKEIIKIIKRKTDKIYIENFTEKSKELGYVRCLNILMLGFASNFIPIKEESF
                     INAINMYVKKEFVKINYLFFKSGRELYHL"
     gene            complement(28090..28959)
                     /locus_tag="PLW95_03135"
     CDS             complement(28090..28959)
                     /locus_tag="PLW95_03135"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOV21659.1"
                     /translation="MRKYEITYENLNSESYLKPITLLVIEPDKINSETGIMFFTHGWG
                     GNRFQPVDRMEYATTNFNVISISTEYRQSGFDFNSITGLGSYVPYDASFFQVFDVLNG
                     LRFFLSIRDINRKRIYHYGGSQGGHICLLSSIFAPDTFTFVYASSPIVKLTNTYIDWA
                     GRYFGDYEISIRDVMEHANLIKCPVFLEHGTNDHVVPHNEHTEELVKELKKLGKNVFV
                     KYYENGGHDLQPTSTRLEAFMCMCKKNIFDFKNENIDDFQKGSIVRIPAGSKTLVIDW
                     SKKISDNNLFFWE"
     gene            complement(28956..29990)
                     /locus_tag="PLW95_03140"
     CDS             complement(28956..29990)
                     /locus_tag="PLW95_03140"
                     /inference="COORDINATES: protein motif:HMM:NF025957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOV21660.1"
                     /translation="MIYKNVELYNVYELVEIGKEKNIISRIPDVLRKHLKQNEYLQTR
                     ALMPDGCEIRFNLKSDIGKIMLQAKDKGAGGIYEIYQGNFFHSFQWISDEITTITLKR
                     EDLLSNSKKLKILTEKNGLPFDPILTRIILPRSIILKIVDIEGNFDIPEKEQVPSIKY
                     LSYGSSITQGQSAIRPTGSYVMRTAQLLGVDLINLGFGGGAHLEKEMADYIAEKVKWN
                     FATLEMGINMLYNFEVEEFKKRVEYFIPTIKEKNPDKYIFCIDIFTFGSDFFDDCNGK
                     EKKFREIVKKTVESLNNEKVIYIPGTEILKNVDGLGVDLVHPSPSGMEEIAWNLSSII
                     KPKIKQENNI"
     gene            complement(30038..31246)
                     /locus_tag="PLW95_03145"
     CDS             complement(30038..31246)
                     /locus_tag="PLW95_03145"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOV21661.1"
                     /translation="MDIIVQKYGGSSLIDIEKIKFVAERIVETKKNGYGVVVVVSAMG
                     KTTDNLISLAKQITTFPPARELDLLLSTGEMVSVSLVCMAIENLGYEAEGFPGYFAGI
                     KTDNVHTKARIQKIDPKNIIRELNKNKIVVVAGFQGISFENTITTLGRGGSDLTAIAI
                     AAAIKAKLCEIYTDVEGVFTADPNIISEAKLIKEISYDEMLEMAWSGAKVMQARAVEF
                     AKKYKVPFVVKPTFNKKGEGTMIKEMEVKEGASVSAVSIDKNQAKITIFRIPDRPGIA
                     AEIFTSIGNENINVDMIVQNVSKQNYADISFTVDINDLEKAFEISQKLGEKLGAEKVE
                     KDTDICKISIIGIGMMNNPGVAGRMFSAIAKVGVNIEMISTSEIKISSIVRKKDGEKA
                     LKQVHKEFIQ"
     gene            complement(31298..33520)
                     /locus_tag="PLW95_03150"
     CDS             complement(31298..33520)
                     /locus_tag="PLW95_03150"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOV21662.1"
                     /translation="MNNVLKFLNYLKNSDLKKHIVYLKTIKEKKGEKYDIPDFISPEI
                     KNYLKENGIEKIYIHQKEGLEKLNDKKNIIITTPTGSGKTLIYNTFVINHILKTPDSK
                     ALYIFPTKALTQDQLKTLKNFRKKFPSITAEIYDGDTPENIRKKIKRKFPSIVLTNPD
                     MLHIGILPFYSLWREFFSELSFVVIDEVHTYKGIFGSNVSHVIRRLRRICNFYGSNPQ
                     FILSSATIKNSESFAGQLVGLDFESIKENSAPSGKKHFVFWDTLEDSPYTQTIELMKK
                     SVENGLSTIVFTNSRRATELLQIWGIKNPSLKNVISSYRAGYLPEERREIEKNLFEGN
                     LKGVISTSALELGIDIGWLDTCILFGYPGSIISTWQRAGRVGRKNNESIVIFVALSDA
                     LDKYFLKNPDELLEREFEDVIINFENEIITENHLKCACFEMPFSENEINLYGNFIKEI
                     LETKFKKTFDGRYFYSGKYPHTQVNLRTVGDIFSIVEVDTGKIIGEIEENKVYYDCHP
                     GAIYLHLGNKYYVSFIKPQTKQVIVQKAKVDYYTQVNWWEKINIMDMIKEKGNEFKIK
                     FGKIEVTTNFVSYEKRKEKDKTLIGLYQLNLPDIKFQTQSLWIEIHEKQIEEFKKRKI
                     DFHGSIHAVEHSIIGIFPVEVPSDRGDIGGYSFPFHQQTEKATIFIYDGYPGGIGITK
                     TGYERIEKILNLSYESVKGCKCESGCPSCIQSPKCGNKNNPLDKRGCLFLLETMLANI
                     "
     gene            complement(33567..34499)
                     /gene="fmt"
                     /locus_tag="PLW95_03155"
     CDS             complement(33567..34499)
                     /gene="fmt"
                     /locus_tag="PLW95_03155"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOV21663.1"
                     /translation="MKIIYFGSDEFGIPSLYELKKNFNLLTIITTPDKPKGRGQKVLP
                     TPTKIWAIENKIEVLTPAEFDENFISKLKSLNPDLIVLISYGKKLPVEILKIPKLCSI
                     NLHPSLLPKYRGPAPIEWCLINGEEETGITVIKMEENIDAGEIIEQRKFPILPDDDAI
                     TLKNRLSNEGSKILISAIEKIKKGERGEKQKGIPSYARKLKKEDGKINWEKSAKDIHN
                     LVRGVIEWPTAYTYIETKNGKKLIKIFKTEIEKESGKFGMIGEIIKIGNDFIEVACGN
                     GTIKIKQLQMEGKKRIETSEFLKGFHYPLTKFTN"
     gene            complement(34496..35443)
                     /locus_tag="PLW95_03160"
     CDS             complement(34496..35443)
                     /locus_tag="PLW95_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOV21664.1"
                     /translation="MKCVGIDLGGTFIKAGLVDDKGNIIKKNQFPTRGEEGKREIVLS
                     QIENAINFCLDGENKNEIIGIGIGTPGLVDDEGIVYGAPNLPGWDNLPLKDIFEKKYD
                     IKVVIENDVNTIAWGEYLFGAGKGYKTMICITLGTGVGGGIVKDGKLLRGEKYSAVEI
                     GHITIDYKGQKCKCGNYGCIERFVGRDYIVERAVNAIKEGKETKIYELAGGNLNKITP
                     KIISDAYNLGDEVAKDIWTDVGICLGALFSSLINLLNPDIIVIGGGISQAGDILFETI
                     RKTIKERAMKKLSENVKVVPAGLGIDSGIISSASLLFTK"
     gene            complement(35440..36693)
                     /locus_tag="PLW95_03165"
     CDS             complement(35440..36693)
                     /locus_tag="PLW95_03165"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOV21665.1"
                     /translation="MISRERILRTLNHKIPDRVPIHDSIWDATIRRWRKEGLPEDMTP
                     SEYFGYELEFFGADTTPKFPVKVIERSEEYIISTTPYGGKRKNHRDYSTTPEVIDWPI
                     TKKEDWKEIKKRLTPDFTRVDWASGLSRNVSAYEEGKFCCFSGGYGYDILQNYIKSEQ
                     LLISMAEGPDWVKEMIMTLAELTIIMAELMMKNGFKFDGAFLYNDMGYKNGPLFSPET
                     YMKTHYQADKLVYEYFHSKGMKVFLHSCGNVKELIPILIDVGLDCLQPLEVKAGMDLI
                     ELKEKFGAQLSFMGGIDVRLMEDPDLTKIEEEIKKKFEVAKKGGGYIYHSDHSVPKTV
                     SFQQYSNIIELVKKYGVYSEYEEISEKEEKIPIKEPMEKSQPLKKEKRGLFRKKEEKK
                     KEVQSPEEKKEKKGFSLFKKKEEKK"
     gene            complement(36683..37108)
                     /gene="tsaE"
                     /locus_tag="PLW95_03170"
     CDS             complement(36683..37108)
                     /gene="tsaE"
                     /locus_tag="PLW95_03170"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOV21666.1"
                     /translation="MGIFYTNSGNETEKLGEKFAKSLKKGDIVGFSGELGSGKTTFIK
                     GIVKKLCNLQAISPSFVLINEYYGKIPVFHIDLYRLENEREIEEINWEEYIGKGIILI
                     EWVEKIKKNLPEKLILVKIEILDDNNKRKIKIKRRKNDK"
     gene            complement(37126..>37435)
                     /locus_tag="PLW95_03175"
     CDS             complement(37126..>37435)
                     /locus_tag="PLW95_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21667.1"
                     /translation="FIKNYEKLFFNRDVKSFFELKTEPQVLNIGYKTNNTFIIHLINY
                     KKEEIKNLKIFINEKNIKNIKLSSPENSNGTILPISEEKMGKFVQISDLKKWAILIGH
                     "
BASE COUNT        13375 a         5240 c         5709 g        13111 t
ORIGIN      
        1 ggtatttaat tcttcatcat cttttttgta ctttatcttc atagatattt tcttgggtct
       61 gttatttttc cttcaattac acttgcagca actgttgcag gtgaagatag gtaaataaat
      121 gcattttggt tgcccattct tcctttaaaa tttctattag cagttgaaat aacattttca
      181 ttatcagaag ggacaccttg atgagttccc acacatgggc cacaaccagg attggtaata
      241 attgcacctg cttcaagtaa tgcatctata tacccatttt tcaaacactc taaataaatt
      301 tttcttgaac caggagtaca tattaatcta acaccttttt tgactttctt ccctttaaga
      361 atttttgaag caatttcaaa atcactggtt cttccatttg tacatgttcc tataaaaccc
      421 tgatgtattt ctattccttc aacctcattt atatctgaaa cattatcaac agtatgtggt
      481 tttgcaactt gagggggaag ttctgaaaca tcaaaattat atattttttc ataatttgca
      541 tcattatctg gaaaaacagg actgtaattt tcaatttttc tttcttttaa aaattccttt
      601 gttttgtcat caaattccat aattccacat tttgctccaa tttcagttgt caaattacat
      661 attgtaaatc tcccatccat agataaattt tcaattgtct ctcctttaat ttcaactgat
      721 ttataaattg ctcctcttgc tgttaattgt cttaccataa aaagggcaat atcttttgca
      781 aaaactccat cctgtaaatt tccattaaac acaattttta ttgtttcagg aactcttaac
      841 cataaatatc ctgtataaag tgcaattgca aggtctgttg aaccaactcc aattccagca
      901 gaatttaatg ccccatatgt aggagtatga ctatcagcac caaaaacaag agagccacac
      961 ttaacaagtc cggtttcagg aagtaataca tgacagacac cttcaccaac ctcaaaaatc
     1021 cttatattat actcctttgc aaagtccctc attagtttat gtaaagcaga aactccctga
     1081 cttggacttg gactattatg gtcaagaaca aaaacacatt tatcagggaa agggattttt
     1141 ttttcagaaa attgttcaaa agaatttata acgaggggag ttgttccatc ctgggacatt
     1201 aagaggtcat attctacaat agaaatatct ccgcttttaa catttttacc tgaatgattt
     1261 gatatgattt tttcaattat tgtttttccc attttttaaa taaaatgttt ttttaaaaat
     1321 tgtattgttt ttctattact ccattgatgt tcaccttcaa aaaaatcatt ttctaaattt
     1381 tcttcttctc caaacatttt ataaaccttt tctaacattt tatatgcttt ttttgtccct
     1441 tcaataggaa aaattgggtc tttttcgcca cttattatta acaaaggtct tggagcaatg
     1501 agagcagcaa tatcgctcat ttctgcccat tccataatat gagggacaca attacatata
     1561 caatgaaata tctggtaaat agaatctcta aaagtacaaa aatacccggc aatcattgca
     1621 agttttattc tctcatccat tgctgataag aataaagatg tagttcctcc tcctgagagg
     1681 cctgcaactc caatttttaa tttatcaacc tcatcttgag tttttaaaaa gtcaacaata
     1741 cgcatacaat cccatactct aactccaata ggtgtcatac ctattaaaaa ggcatccata
     1801 gttactctat aacaatctcc atattgagaa ggttcagcat accttgtttc attccatcct
     1861 ctttgaatag gtgctattgt tatataccca ttttctgcaa aaaaaggagc ataatctaaa
     1921 gaatgccttt tccctaatgc tttttgttct ttagtacctt cacgaatata aggtaatatt
     1981 cccctatgtc catgaagaca aattattgct ggggtttttg cttttaattt ttttggtaaa
     2041 aagtaatagg aaggaatcca ataatctttt tcagagcgga tataaatttt gtttcttata
     2101 aatgattttg tttcaatttt ttcatcccaa tcaacttcta aaggaacttt ttttggcatt
     2161 ttccccaata attcttgaag ttttgactca agtttttttc tccagatata atgggttgaa
     2221 attgtttttg cattccagga aagttttgga gaaatattat caactacttt catgaaataa
     2281 ttaaatgtat tactatttct ttgcatatat ttctcctatg gagtcattgt caaaatttgt
     2341 tttacttctt caggatattt tgctataaat attaattcgt cactaactaa tggtttttgt
     2401 ttatgaacat ttgcatatct ggctaattct aatatctctc ttgcttcttc ttcatcttta
     2461 aattttattt ctaattttcc ataaacattt cttaaaccct tgataccact atattctcct
     2521 aatattatca ttcttcctgt ttcaatgatt tctggttctc ctcttcctaa ttcttcaaaa
     2581 ttgtataatt cataatttct tctatcttta agtgcaccat ctacatggat accagaagaa
     2641 tgagcaaaag cgttttctcc aactcctacc tgatttattg gaattggaac tctaaaagca
     2701 taagaagcat aatgggatat tttccatgcc atctttaaat ctactttttc atctaattta
     2761 tattttcctt caatccctga ggcatatttt atagcaagaa taacagaaac aaggtcagca
     2821 tttccagttc tttcacccaa tccatttact gttgtgttta tataagcatc aactccacca
     2881 tcaatggctc cttttgctcc cgccacagaa caggcaacag ccataccaat atcattatgg
     2941 cagtgtagtt caattgggat tttcacttct ttagcaattt tataaattct ttcataaatt
     3001 gaaaatggag aatcataacc aagtgtatca caatatctaa ttctgtttgc cccaacttct
     3061 tttacagcag aagcaaattc aattaaattt ttaaggtcag ttcttgaagc atcttctgca
     3121 tttactccta ttgtttttgc tcctttttct tttgcggatt ttactgcatc acccatattt
     3181 ttaattacat ctttaaactt cattttccct tgaaacttgc ccactatcat ttgatccgaa
     3241 gttgatatag aaatatttaa gtgttgtaaa tcagggacat ttttaaaagc attttcaaca
     3301 tcagaaggta ttgctctgag ccatccagaa aggcgtatag gatatagaca tttctttttt
     3361 acaagtttta agttagcatt taaataattt atttcatgag atgtaagtgg aaaaccaaat
     3421 tcactctgaa aaattcccat ttgatttaga taataattta tcatcgtttt ttcaagtttt
     3481 gcaagaccaa gtcgggccgt ttgaactcca tcacgatttg taacatcaat tatgtaaatt
     3541 ttattcataa ttttcccccc ttaaaattca ataacttctg gcttttgtgc ctcgtgatga
     3601 tgattggttc cacgataaac ttttaatttc ttaatcatct gtctaccaag tttattttta
     3661 ggcaacatcc cccaaacagc atgttttata acaaattctg gctttctatt aagaagagaa
     3721 aataaatttt ctttttttaa tccacctaaa tatccggaat gtttataata aattttatca
     3781 accaattttt tgccagtaac tttaattttg tctgcattaa tcacaataac aaaatcacct
     3841 gtatcaacat ttggcgtata aattggttta ttttttccca taagaattcc ggcgacttta
     3901 cttgcaagtc gtccaagtat ttttccttct gcgtcaacaa cataccaatt tctttttatt
     3961 ttttcttttt tagcaacgga tgttttttcc attttttccc tccattaagt cgtttaatta
     4021 tacttaaaaa aagttcttcc gtcaattttt atataggatg ttttagaaat atttaattta
     4081 aatattatta agtaataatt taagatatga aaaaataaag ataataatta cacttaaaat
     4141 aataagaaaa acaatccata taattatctt ttttttatca aattcgggaa tatctttgta
     4201 tttatcttcg taataatcac caacaatttt tgctatttct tcaggacttt ttcctttaag
     4261 tttctccttc cactcatttg aattaaaatc aatattgtta ttttctttca ttttgacata
     4321 tattatttta tatgagaaaa taaaaaaatc aaattttaga gttgaaagga ggaaggaaaa
     4381 atgaataaag taagaattgg cgtaatagga gttggaggga tgggctctgc tcactgttta
     4441 attataaaag aaattgaaga gggtgaatta acttgtgtat cagatgttga agaagaagtt
     4501 gcaaagaaaa aaggagaaga atttggagta aagagtttta ctgattatag ggaattgata
     4561 aaaagtggac tttgtgatgc agttattgtt gcaacacccc attggtttca tccagaaata
     4621 tctgtttttg cttttgagaa tgggctacat gttttaagtg aaaaaccaat tgcagtcact
     4681 gtttctgctg ctgatagaat gattgaatca gcggagaaga ataataaaat ttttgctgtt
     4741 atgtatcaga gaagagcaga atggttgata agaaaagcaa aagaaatagt tactaatggg
     4801 aaaattggag aaattataaa agcagtttgt attgacccat ggtataggtc tcaagcatat
     4861 tataatagtg caacatggcg cgctacatgg attggtgaag gtggaggggt tttaataaat
     4921 caagcacctc atacaattga tatttttacc tatttagcag gattgccatc taaagttgaa
     4981 gcaaaaacga ggacaaaatt acatgatatt gaagtagagg atgaagtatc tgcattttta
     5041 gaatatagaa atggtgcatg gggttattat tacacttcaa catgtgaacc agttggtcct
     5101 ttttatttag aggtctctgg agataaaggg aaaattgtaa taagagggga ccagattact
     5161 ttttacaatt atgagacacc aataaaggaa tttacggaaa aagcacctga tatgtgggca
     5221 tcaataaaaa cagaagagga aaaaattatt gcctcttcaa attttgaaac aggacataag
     5281 gtaataataa aaaattttgt tagaaatata ctctataatg aaaaacttat tgcccctgga
     5341 aaagaaggac ttaatacaat tgaatttatg aatgcatgta ttttatcagg gaagaaaaat
     5401 aaagttgtta atatacctgt tgatagaaaa gaatatgatg atttaattga aacattgaaa
     5461 aaaacttcaa ggggaaaaaa agtagtaaaa atacagagag ttactgaccc caatattaaa
     5521 aagtagaagg gacttggagt agataataaa gcacactttc tacaatatat gaatgagtta
     5581 ggtaatattt ctctgggcag atgtatgctt tataaggaga atgaatgaga ggtaagattt
     5641 tatctgctat ttttttattt tttcttgttc tttcaccatt ttttctaata aaatatagaa
     5701 attcaacaaa aaaggaaaag gaagaaaaat atagaattat tgatggagta attttcacag
     5761 gagattgtat aattaacatt ttacaacaaa gtggtatttc ttctaatgtt gccctatcta
     5821 ttgcaaataa attaagtact gttttcaaca caagaagttc aaatattggt gatagatata
     5881 gaatttattt tgataatgaa aatgggaatt ttataaaatt tgtttattat agcgtaccat
     5941 tagattttta tactgttcaa tatgatgaaa aaaataagga tttttttgtt tcatcaggga
     6001 aggtaaaagc agaaaaagag atattgggag taaaaggaga aataaattct tccctttatg
     6061 atagtatgat aaaacaaaat gtgaattcgg aaataataat acaatttgca gaaatttttg
     6121 cctcaaaaat tgactttttt actgattgca agaaaggaga taagttcgtt attctctggg
     6181 aatcatataa agataaaaaa gggaatattt taaaagatat taaggttgtt gcaggggaat
     6241 atataaatta taataatact tactatgctt tttattttga accacaagat aataaaaaag
     6301 gtggatatta cgatgaaaat ggacaaagtg ttgaaacagc atttttaaaa tcacctttaa
     6361 attatagaag gatttcatca tatttttctt atagaagatt tcatccaata cataaaattt
     6421 atcagccaca tttaggaatt gattatgttg ctcctgctgg gacgcctgtt tcctcaatag
     6481 gtgagggagt tattattttt gctggattta gtaataatgg atatggaaat aatgtcaaaa
     6541 taaaacatcc aaatggatat atttcctact atgggcatct atcaaaaata aaaaagggaa
     6601 taaaaaaagg tattagtgta aaaaagggag aagtaattgg ttttgttggg atgacagggc
     6661 ttgcaactgg acatcattta gattatagaa ttaagaaaaa tgataaattt ataaacttct
     6721 taaaattaaa attgccacct tcttacccaa ttgataaaaa atacattccc gaatttgaaa
     6781 aaattaaaga gcaatatatt gagaaaatgt ctacttttta aaatttccct tatacaatgt
     6841 tataattttt ctatgaaaaa aggtttttct tttttagaaa ttgttattgt aattttcatt
     6901 ttttcaattt tacttattcc aacaacattt gtaattctca attatcaaaa aaatttatct
     6961 cttgaaagtg ctgtaagtga aatagttgaa gtgcttgatt atgcaagaaa ttgtgctaag
     7021 agtgaaagag aaaaattttt agtaaaattt gaagataaaa atttttatat tataaaaaat
     7081 ggaaaagttg ttaataaaat tcaacatctt ccacaacata tatatttcaa agaaattaca
     7141 gatggattaa atcctgtcgt ttttttacct gatgggacaa cagaaatggc agggcatata
     7201 attttaggaa ttgaaaataa aaatgatgaa aaaaagatag ttgttaataa tattacaggg
     7261 aaatgtataa taaaatgaaa aaaggaataa ctttaataaa tatccttgtt tctcttatca
     7321 tcctttcaat agggataatt actctattaa aggtctatcc agttattagt tcactttctg
     7381 aaaggggaaa agataatgca actatttcaa tgatttctac tcagattttc actctcattg
     7441 ataaagttta ttcttcttcc aataatgatt tgccttcttc tgttgatggg tgttttcttg
     7501 aatttccaca atacaaatat aaaattgatt tttcagaaga aagaaaaaat ttatataatg
     7561 cggatattga aatttcatgg aaaagaaagg gaaaaattga aaaaagatat tttacatatt
     7621 cattcagaag agaataaagg gtttacctta attgaggttt tagtttcaat tgtaattctt
     7681 acaatcatta ttttttcttc tatgtttatt tttaaagaaa gtgtaagaaa atttagaaaa
     7741 aatacatatg aaaaagaaat ttataaaaca gcaacagaaa catttcagac aattgaaaaa
     7801 tatcttaaaa atgccaaaat aaacaattta aatggaaaat taagaatgaa ctttaaaggt
     7861 cagaaacaat gggtcaaatt tatatctacc acagaagaaa gtaaagagag tgattttttg
     7921 aaagtcggga tatatttcca ggataataac ttaaaaatgt ctattgaaaa aattgatgaa
     7981 gaaagtgatt ttagttttaa gaaaggtttt ccgggggcac aaattcttgc agaaaatatt
     8041 agaaatttcg aaattttata ttttgatgga acaaattatg tttcaaactg ggatacagaa
     8101 aacacagaag agccccaatt acctccattt gttaagatta ttatggaaat tgaatcagga
     8161 atgaaagttg aaggaaaaaa attaaataaa aattttgaaa gaataataaa ggtgggttat
     8221 gaatagagga gtaagtataa tttttacgat ttttatttta tttcttcttt ttatttttac
     8281 tgcaacaatg ctttttattt ttcagttctg ggaaaaaaca agttttaaat ttttttcatc
     8341 aagacaggca tatacttttg caaaaaatgg catagaagaa ggtatatggg aaattaataa
     8401 tgatgataaa aaatatgatt gtttaaatga taaatggaga gtaaattttc agggaaaaga
     8461 agttgacctg aatgaagatg ggaaatttga ttcaaaatgg ttctatataa aaaataaaaa
     8521 aggagaaatt attggtagat atgctgtttt agttgaagat gaaagtggaa aggtaaatat
     8581 aaattcttct ggaaatttat ctttaaatta taacgaaggt tatggttgtc atgaaatatc
     8641 tctttttcgg gaaataatag gggaaaagaa tatgataaat atattgcatt ttaaatatgg
     8701 gaatgacttt ttccctggaa caagaaattt tgatgataat aaaaaccaga atagttatga
     8761 aacagatgga attgataatg atgctgatgg aattattgat gagtatggag aaggaattga
     8821 tgaaccagaa gaatttaatt ttgaaaaacc ttatggggat gatagaccat tttttgatat
     8881 ttcagatatc aaattagttg atggaattgg acaggatgta ttcaatagat ttaaaaatta
     8941 tattacagtg ttttcttatg accttgaaag aaacaaaaat gatgaagaaa ggataaatat
     9001 aaatactgct gatttcaata cacttttttc tttttttcaa tcacttggat attctgaaaa
     9061 agaggcctgc caaatttcct taaatattat tgattacaga gataatgatt ctatacctac
     9121 aataaaaaaa ttaggcaatt cactatttat aggaattgaa gatactcctt acttgaatga
     9181 aatagatgca gtgaaagaat gggaaaataa aacacttgaa tcaggggcaa ttattcttac
     9241 tgaaaaaggt ggacaatttg ttgaaatttt taatccttat gacagagaaa ttgatattgg
     9301 cggttgggaa gtaaaaggtg ttttaaccct tttttcaaat ttatggaatg gtattttttc
     9361 tacttccgag caagttttgg atgaagttga aaatggggaa acagaaatag tacaaccaga
     9421 ttttcttggg aatcttgttc caactacaat aaaaataaga gaaggagtaa aaattgagcc
     9481 acactctttt tatacaatag gagaccttat aacaattgta ataataatgg tccctcctga
     9541 ctttgttcca attattttat ttttacctac acatgaccca tctgattgtt cacaatatga
     9601 accaattatt gctataaatc ctggttcttt tggtgctatt tcaaaaattt tatcttctat
     9661 tccctttctt tcaaaattag gacttgattt cacagtaagt ttatatgaca aaggtggaaa
     9721 tttaattgaa aaaacggact atccgcttga tacaccatgg acaactgttc agaaaaatga
     9781 cccaagaatg agcggaagtg gtaattggtt tccttcaatt tctactccaa atacctttaa
     9841 ttctgttttt atgccatttg taggtgaaga atttaatagt gttaattgga ttttaaactg
     9901 gggttcttct tttgcaataa aaaataataa attttcttca gttggagaaa tttcacttat
     9961 acacaaaaac gcacaatgga aaacaataaa tttatggaaa aatatatcgg acagaaaaat
    10021 tattgataat ataaaagtta gaaaagaaga aaaggtctac gggaaaataa atataaatac
    10081 aagtagtgaa gatactttaa aatgtctgcc attggttagt aagataactg caaaaattat
    10141 ttgtgcgggt agaccctata cagatataac agaaatactt ggttctcctt ccggaatgaa
    10201 ttttgaaaaa gttcttttat ccagggacat tacaaaatat ggatttgatg ggattgataa
    10261 tgatagagat atttatattg atactgaaaa ggaaaaagaa atggtgtttt caaaaattgt
    10321 aggtctcata acagtaagaa gcaacatatt taaaattact tctattggag aaaaagtgca
    10381 agataaaaat aataatggta aaatagaaga tgaagaaata attgcaacaa aaaaaataaa
    10441 tgcgtggtat gataggagaa aaaagaaaat aatccataga aaggaaattt aatgggtaaa
    10501 ttgaaaattg aaagcggaaa atttacaatt gtaaaattaa aaaatttttc taagaaacat
    10561 gagaaaaaaa ttattttatt ctttttatgt attcttcttg ttttatttgc acttcttttt
    10621 ctccacttta aaaaaataaa agatgaaaca gtggcagttg taaatggata tagaattact
    10681 tttgatgata tactgacaaa agcagaaaca tctccagtat tttatcagga atatttaaaa
    10741 attgaaccaa aattttttgt tgaggaatac ataaatcaga ttctcctctt tcaaaaagca
    10801 aaacaaaatg agagaaaata taaaaaagaa attgaagaga aaatgaaaaa ttactatatt
    10861 gacttactca caaacattta tgtagaaaaa gagttaacag aaaaaataaa agtaagtgaa
    10921 gaagaaatac aggactatta caacaaaaat ataagtgatt ttgttattcc tgaaaaagtt
    10981 aatctatatg aaattgttac agatagtaaa gataaagcag ttgaaatttt atcaagatta
    11041 tccattggag agtcatttga agatatagca gaaagagagt caataagtaa ttcagcaaaa
    11101 aatggaggta atttaggttg gattgatgta gaaaaattag accatgaaat tgcatctctt
    11161 gtaacaaaga taagtccagg gcaaattcta gcaaatatct ttaaaacaga catgggatat
    11221 catattataa aactcgctgg caggactgag aggagagttt taacattgga agaggcaagg
    11281 caatcaatta taaatatgat aaagacccag agaaagaaaa aagaagtaga gaaatttgtt
    11341 aaaaatttaa gagagaatag caaaatacaa atttttcctg gtaagataga ggaaataaag
    11401 acaagaataa aatgaaaaag attatattta tttttgcttt ttttgtacca tttttgtttt
    11461 ctgctgataa aatagttgca tttgttgaaa attctcctgt ttttgaaagt gaggtctacc
    11521 aaattatttc ttatgataaa ctttcttatg atgaagcact tgaaaaagta attcagagaa
    11581 aacttattgt tgaaaaaagc aaaaaagaag atatcaaggt aagtgatgaa gaaattggaa
    11641 aagaatttca aaaaataaga caatcttttc cagatgagaa aagttttatg gaaaatctta
    11701 aaaaaaacgg attaacaatc tcacaactga aaaatataat aaaagaacaa ataatatcaa
    11761 gaaaatatat tgataaagaa ataataagca aaatagaaat tccaccgtca gaaatagcaa
    11821 attttataaa caatcattta gagataacaa aatacaattt taaatttaag tggtttgaga
    11881 caaaaaaagc agcggaagaa ttttgtcttt cacccgatat tacaaaaatg gaagaaagtg
    11941 gatatttaaa agaggatgaa attttatctg aaatacttac aaatttaaaa aaaattggta
    12001 aaggtgaatt ttcttctcca tttgaggttg gtaataaatt tattgttgta tatctttcag
    12061 atgtaaaaaa agaaaaagta gaaaatttaa aggacttata tattacagca agaagaaaaa
    12121 tttttaatga aaaatttaat acaatgtata agcaacttgt ttctaaatta aaaaatgaaa
    12181 tgcttgtccg aataatagaa tgaaaataac aggaataaca cttggagacc cagcaggaat
    12241 tggaggagag atttttctta aaggttataa tgaaataaaa aaaataaaaa attcatttcc
    12301 tgttttaatt ggtgatagaa ttgttcttga aagaaatgcc acttctttaa gtcaaaaaat
    12361 atctataaaa gaaataaaaa acaaaaacca gatagataaa aattgtataa atatatttcc
    12421 tgttaattat ataaaagaaa aaaattatcc gattggaaaa aactcaaaaa tatgtggaag
    12481 tgcctctttt aaatatattg aatttgcaat tgacctatgg aagaaaaacc aaattgatgc
    12541 acttgtaact ttgccaattt caaaaaaagc atgggaaaaa gcaggaatta aatatccagg
    12601 gcatactgaa ttattatctg aaaaatttaa tgccagaaaa acagcaatga ttatggttgc
    12661 tgataaaata atgacagttc ttgccacaac tcatatttct cttaaagagg caattgggaa
    12721 attaagtgtt caacttatag ttgaaaaagt aaaaaatggg attgaatttc ttgaaaaaat
    12781 aaaagttgaa aatcctgtta ttggtgtttc tgggttaaac cctcatgcag gagaagaagg
    12841 acttatggga gatgaggaaa taagaattat aaaaccagca attaaaaaat taagaaaaca
    12901 aggaataaat tgtattggtc ctatagttcc agatgcaata tttagaaaaa atatagaagg
    12961 agaaatttct cttattattt caatgtatca tgaccaggca ttaattccac ttaaaacatt
    13021 ttattttgaa aaattggtta actttacatc tggaatacca atgataagga ccagccctgg
    13081 acatggaact gcttttgata ttgcatataa aaataaggga aatccatcaa gttttattga
    13141 aagttataaa ctcgcagttg aactattgaa gtaaatggat aaatttctta cgaagaaaaa
    13201 attaagagaa cttttagtta gtgagaattt agatataaaa aaatatttag gacaacattt
    13261 tttagttgat agaaatgcaa gagataaact tctttcattt gcagaaattt caaaagaaga
    13321 tgttatattt gaaattggtc ctggacttgg ggcacttaca gaatatctaa ttgaaaagtc
    13381 aaaatgtgta tatggttttg aaatagataa ccacctttgt aaaattttaa ataagagatt
    13441 tggtcattat aaaaacttca atcttataga gacagatttt ttacagacag atgaaaattt
    13501 ctggaggaat tttgaaagag taaaagtaat tggaaatact ccttactatt taagttctcc
    13561 tattgttttt catattctca aattcttaaa aaaaatatct ctatctcttc ttacagtcca
    13621 aaaagaagtt ggagaaaagt ttgttgctca tccaggaaat aaaaattatg gtcctatatc
    13681 aattcttctt tccctttaca cagatagcaa aatatgttat tctttaaaaa aagatgtttt
    13741 cttcccaaaa ccagaagtac agtcagttgc agtaaaaatt attcctttgg aacagccaaa
    13801 aattgaagtt gaaaaaaaat tctgggaatt tttaccatta atttttaata atagaaggaa
    13861 aaaattagtc aatgtaataa ataaaatttt taaattatca aaagaagata ttataaaaat
    13921 aataaataaa attggtattt cagaaaatag cagagtagag gagttagaac cagaaaagat
    13981 atatcaactc tatcaaatgg taaatataca agcaactgtg tttcctgaga agtagaaagc
    14041 agtaagtaga aagagacaaa aggcaaaatt ctcttcatct ccatttgtaa agggaggatg
    14101 aagtgaattt ttagagagta gaacaaatgg gtgataaaac atatttaaat agaataattt
    14161 gtggtgatac tttaagtatt ttaaaaaata tgccagatga aattgtagat gtaggtgtga
    14221 catcacctcc ttataataag ggagaaaata aaaaggggtg gttagtatca gacttgtttt
    14281 actatatgaa gaatgaaaaa atttgatatt tttctggcag acctggtgtg gagaaaacaa
    14341 ttaattaaaa aaggagagga aaaatggcaa tgtggatagg aataaatagg aaagcacgtg
    14401 tttgttatgg atttgatgat gtttctcttg ttccaggaga tgttacttta aatccagaag
    14461 aagttgatat ttcaacaaaa attggaaatg ttgaattaaa aatacctgtg cttgcagcag
    14521 caatggatgg agtggttgac cctaaattcg cagttgaatt tgggaaactt ggtggaattg
    14581 ctgttttaaa tcttgttggt atttatacaa gatatgaaga cccttatgaa ataattgaga
    14641 aaataatttc aagtccttat gaagaatcaa caaaaattat ccaaaatatt tataaagaac
    14701 caataaaaga aaatcttata ggagaaagaa tagagcaaat aaaaaaggga aatgttgtct
    14761 gtgcagttag ttcaattcct caacttgcag aaaaatatat tggtattgca attgagagtg
    14821 gagtggatat ttttgttgtg caatcaactg ttacaactgc aaaacatatt tcttcttcat
    14881 ataaaccact tgacctttat aaattctgta aaggagcaaa agttccaatt attataggta
    14941 attgtgtaac ttttgatgtt gcttatcaac ttatggaaac aggagcagat ggacttcttg
    15001 tagggattgg gcctggggct gcctgtacta caagagaggt tttaggtata ggcgttccac
    15061 aggtaaccgc aacttctgat tgtgctgctg caagagataa atactttaaa aagacaggaa
    15121 aatatgtctc tgttataact gatggaggaa tggttacaag tggtgatatc tgtaaagcat
    15181 ttggagttgg ggcagatgca gttatgattg ggtctgcttt tgtaaaatcg gtcgaatcac
    15241 ctgggaaagg gtatcactgg ggaatggcaa catctgatgg aaatttacca agaggcacaa
    15301 gagtaaaaat tgggacatcc gggactttaa aagaaattct ttttggacct gcaaaagttg
    15361 atgacggttc tcaaaattta atgggtgcat taaaactttc aatggcatca gtaggagcaa
    15421 aaaatatcaa tgaaatgcaa atgacagata tagcaattgc tccatctatg aaaacagaag
    15481 gtaaaattta tcaggcagca agaaaagaag caagaaaata atcacaaata ttaaataaat
    15541 aaattggtta agagacacaa aggtacagaa gggaaaaaca aaaagcaaaa tccatcccct
    15601 ttacaaagat aaatgggcaa gcattaaaaa gagtaggaga gatacttccc tttttggtaa
    15661 accgactaag gaagcagggg ttagcaaggc agagttggtg cgttatccca aatactttcc
    15721 ctaaaaatgc ttcccccttt aaccaaaggg ggatagaggg ggattttaga aaattacagg
    15781 aaatccatcc cttccttcct ttgaaaaagg aaggagagat acttccccct tttattaaag
    15841 cctgtccgac gcgtccgcca aaaagcgcgt cggattcgta atgttttgtg gcgaacattt
    15901 tggcggaggg atgaaggggg atttcatcct tctccccagt ctctgtccgc cgtgcggtgt
    15961 ggcggagggg agaaggtagg gatgaggggg attaatgttt ctcacttttg taaaggagga
    16021 ttttaaagta aatttttctg aactttttac ctaaaataaa acggaggtta gtaaatattt
    16081 taaaatgagt aaatttgttc atcttcatct tcattcagaa tatagtttat tagatggtat
    16141 gtgcaaaata gaagaagtta ttccattagc aaaaaagaat aaaatgactt ctcttgcaat
    16201 aacagaccat ggaagtatgg caggagttgt taaattttat aaagttgctc tggataatgg
    16261 gataaaacca attattggtt gtgagatgta tattgcccct gaaagtatga ctgataaaac
    16321 caaaagagaa aggaataact atcatttaac acttcttgct tctgataata atggctataa
    16381 aaatttaatg gctctttcta ctttttctta tctcaaagga ttttattata aaccaagaat
    16441 agataaagaa attttatcaa aatactcaaa agggattatt gcaatgagtg gttgtcttca
    16501 aggggaaata ccatattttc ttttgaaaga tgaaattgaa aaagcagaag aagttgttaa
    16561 tgagtatatt gaaatattcg ggaaagagaa tttttattta gaaataatgg ataataatct
    16621 acctgaacaa aaaaaagtaa attctgatat tttacaactt gcaaaaaaat cagggataaa
    16681 ggtagttgca acaaatgatt gtcattattt aaaaagagag gactcttttt cacatgatgt
    16741 tttattatgt attcagacaa aaacacatat tgaagataca aaaagattaa aattcaagac
    16801 agatgaattt tatttcaaga catcagaaga aatggagagg tcatttaaag atataccaga
    16861 ggtacttgaa aatacattag aaataagtga aaaatgtaat gttgaaattg attttaataa
    16921 gtataactta ccaagatttt atccacctga aaacaaaaca cccgatatat atattaaaga
    16981 aatggtaaaa aatggacttg aagaaaaatt taatctcaaa aatgttaatt tttcagaaaa
    17041 aaatgagata gtggaaaggg caaaatatga attagatgta ataaaaaaaa tgggattttc
    17101 aagttatttt cttataataa gtgatattgt taattacgcc aaaaaaaatg gaataagtgt
    17161 tggtccaggt agagggtctg ctgctggaag tttaatttca tatcttttag gtattacaga
    17221 tataaatcct ttagaatata accttatatt tgaaagattc ctgaaccctg aaagaataag
    17281 tatgccagat attgatattg atttttgtga tagaagaagg gaagaaataa ttaagtatat
    17341 aagagaaaag tatggagaaa acaatgtttc tcaaatagga acttatggga caatggctgc
    17401 aagagcagtc gtcagagatg ttggaagagc attaggcatg ccttataatg aagttgaccg
    17461 aattgcaaaa cttataaatt cttctcctgg tactcttctt aaagaagagg ttgttttaaa
    17521 tccagaagta aaaagagcga tagaatcaga tgaaaatgta aaaaatcttt ttgaaatatg
    17581 tcttaaatta gaaggattag caagacattc ctcaattcat gcagcggggc ttgtaataac
    17641 tgaaaatgag gtatataact attctcctct ttttattggg ccaaaaggtg aagtagcaac
    17701 acagtatgaa atggattgga ttgagaaaat aggacttttg aaggttgata tacttggact
    17761 taaaacatta tcagtaattg aggatacaat agaacttgtc aaaaaaagaa aaggtattga
    17821 gataacttcc tttcctcttg atgataaaaa aacatataaa ttactatcca atggtgaaac
    17881 agcaggagta tttcaacttg aaagtaaagg tatgcaaagt ttattaaggg acatgaatgt
    17941 agaaaatttt gaggatatta tcgcagtgct tgctctgtat agacccggtc cgatgaaaag
    18001 tggaatggta aaggaatata ttgaaaggaa aaaggactcg tcaaaaataa aatatgacca
    18061 tccacttctt gagacaattt tgaaaccaac ttatggagtt attctttatc aagaacaggt
    18121 tatgcagata gcaaataaat tagcaaattt tacaatggga gaagcagacc atttaagaaa
    18181 agcaatgggc aaaaaaatat cttctgaaat ggagaaattt agagaaaaat tcattttagg
    18241 agcaaaaaaa aatggagtaa aagaagaaat agcggagaag atatttgctc aaatatcaaa
    18301 atttgctgga tatggtttta acaggtctca tagtgcctgc tattctttta ttgcatatca
    18361 aacagcgtat ttgaaaagta attttccttt ggaattcatt acatctttac ttaatagtga
    18421 aattggcaat agtgataaaa tagaggaata tataaaagaa tgtgagagat atgatatatg
    18481 ggttttacca cctgatatat gtgaaagcga tggtgagttt aaaatttttg gtaatgatat
    18541 aatttttgga ctttctgctg taaaaaatgt tggttctggt gctattaaat caataattga
    18601 aagcagaaaa gaaggtgatt ttaaatcatt atttgatttc tgtgaaagag taaatttaag
    18661 ggtagtcaat agaaaagtga tagaaagttt aataaaaggt ggagcatttg attatttaga
    18721 aataccaagg tcccatcttt ttgcaatgat agatgatgca attgagtatg gaacaagagt
    18781 gcaaaagtca caggagaaag gagaaattga aatattttct actcctgtga caataaatta
    18841 tacgaataaa aaacttattt cttctttgcc tgaatggtct gaattaaagc gattgtctta
    18901 tgaaaaagat atgttaggtg tttatctaac aggccatcca gttgaaaaat atctgccttt
    18961 attaaaagta tattcatcta ttcctatttc tgaccttcca aaagttaata atggggaggt
    19021 tgtcattgca ggaattttgc aagaattaaa gagaaatata acaaaaaaag gggagaggat
    19081 ggcatctggt gaacttgaag gaattgatgg gaaaatagaa gttatttttt atcctgaaac
    19141 atttaaaaaa tactcatcag ttataagaac taataatatt gtatttataa aagggaaggt
    19201 tcaaaaaaga gaagatgata tcaaatttat tgcaaacgaa gttattgatt tagcagaagc
    19261 aaaagaaaag ttcacaggca caattgaaat tcatatatgt ctgccagttg aagataagaa
    19321 aattaaagaa ttaaaggaat ttatacaaaa caataaagga agttgttctg ttattctgac
    19381 attatataaa gaagataata gaaaagttaa aataaaagca aatggatatg gaataaatcc
    19441 agaaccagat attttactta atttaaaaaa ttcatttcct gatttcaaaa ttaactttgg
    19501 tagataaaag ggaaacccat ccccctcttc ctttacaaaa gggaggagta tatttttctc
    19561 cctttataaa agggagatta agagggattt taagggggat ttgaaggggg gatttttttc
    19621 aatatcagac ctttgtgcct aaaacaaaaa tgggacttat taaatgttta ggggacttaa
    19681 atgaaaccac ctattgaacc aattatctgg aaagaagagc atctttatat aattgaccag
    19741 agaaaactac ctttaaaaga agaaataatt gagataaaaa atataaaaga tgtctgggaa
    19801 agcataaaca aactgaaaat aagaggcgct cctgcaatag gatgttttgc tggctttgga
    19861 attgccctgt cttctttaaa atgtaaagaa aaaacaaaag acaaattcat taaaaaaata
    19921 aaaaaagata ttgaatatct aaaaacatca agaccaactg cttataactt attttatgct
    19981 cttgaaagaa tagaaaaagt tttagatgaa aacaaaacaa ttgatgagat aaaaaaggat
    20041 attgttgacg aagcaaaaca aatatacgag gaagatattg agtgttgcta taaaatagga
    20101 aattatggtt cttccttaat aaaaaatggt atgaatatcc ttacacattg taatgcaggg
    20161 ggacttgcaa caagtggata tggaactgct ctttcgcctc tttttgtatc aaaagaaaga
    20221 ggcaaaaaat tccatgtatt tgttgatgaa acaagacctg tgtttcaagg agcaagatta
    20281 actacatggg aactggaaaa gtcaaaaatc ccatacacct taatttgtga taatatggct
    20341 gcttttttaa tgaaacaggg aaaaattgac atagtaattg ttggtgcaga tagaataacc
    20401 ttaaatggag atactgcaaa caagattggc acatataatc tttcagttct ggcaaaacat
    20461 cataaagtga aattttatat tgctgctccc ttttctacat ttgatttttc cttaaaaaca
    20521 ggcaaagaca taccaattga agaaagagac cctgatgaaa taagaaaaat aaaaaatatt
    20581 tatattgctc caaaaaattg tcctgtttat aatccctctt ttgatataac tccgggtgaa
    20641 tttgttgatg gatttataac cgaaaaaggt ataatacacc ccccttttaa caaataatac
    20701 tcaatagttc cagaaagcat tctgaaagat ataaaaagac atcgcccctt ttaaagaaat
    20761 ctttaaaaag tgataaaata acaaataaag gtttaataag aatttctcta cattaactat
    20821 agtgaaattg atgatgaaga gtatggagtg agtgaaaacg acaaaaaatt aaataaggag
    20881 ttttataaga ggtaactatg ctctatgaaa cttttaattc aataaaagaa atcgttatcg
    20941 caggattgaa ttgttaaata ctatttttaa tagatgttaa atgttgagca caatgagtgt
    21001 aaatcttaaa cattttaatt ttcatttagt ttgtgttata atacaatttg aatatctgtt
    21061 taatgtgtaa tataatacaa aataatgttg aaatatcgga aagaataaga aatgtaattg
    21121 atggaataat taccaaggat ataataactc tcaaaaagtt taagtcagaa acaatcagta
    21181 aaataaataa cttgctttat ttacttgcga attccgatgt aattagttat gaaaaattac
    21241 ttcatacctt acgaattgat aatataagaa cattatcttc tttattggat gttttagtaa
    21301 tggcaggagt tcttataaga gttaaatgtt atggacaaac ttatggaacc accagaaaaa
    21361 caccaaaata tctttttatt acaccctctc ttaggaatgc tattctaaat ggcagtttta
    21421 cttctggact ggaaggaaaa aaatagaaga ttattttgca ttaatttttg aaaatgattt
    21481 aaagaaaacg gttgctcaaa atttatatta tgatatagca aaaagtggtg cagattttgt
    21541 tttaaatttg aagaatagaa gtaatattgt aattgaagtt ggttttaata aagaggaaac
    21601 cagacaggtt gaaaaaacaa tgaaaaaggt gaaaagttct tatggattag tctttgggag
    21661 cgataaatta gaattagtta acaaatcaat tgtaaaaatt cctttaaaat ttttaatgtt
    21721 aatttgataa aaaaaatatt ttaggagaaa aggaaagggc ggttagctca gtggttagag
    21781 cgtccggttc acatccggaa ggtcacaggt tcaaatcctg taccgcccat tggaggaccg
    21841 acttcctatg gaggtctgtt ctccttctgt ataattaaaa attttttatg gactttccta
    21901 cctatttaat tgcttctcaa gggtggttat acgaacttat aactgttggt ctccatgctg
    21961 tttgaagaaa actatcctaa atgaattaaa taacaccaga ttaattcttt ttccattttt
    22021 atgtactttc tgctatcatt cttgaaattc ttttttaatt ttactttacc atttttataa
    22081 aaaaagtgaa attgataatt atttaacaat caatccatat gtctttacaa aataaaaaaa
    22141 atgtgtgata atacaataaa gataattttt aacaacatct tgagaaatgg aggaaataaa
    22201 aaatgggagc aaatataata agagttattt ttttgatttt aagtatttta ttgtcttatt
    22261 gctttttccc ttcaaggaag gttattggaa ttattattgg tctacttgga ggaggtctta
    22321 ttatttttat tgaaatactt cttgaaagga tttcatttaa aaagttatta cttgcagtag
    22381 gtggtttaat tgtaggactt ataactgcaa ttcttctcac aagatttttt ctacttattc
    22441 caatagagaa tccgcaaaca gagaattatc tgagatttat tctttatttt atattttcat
    22501 atttaggaat tatgttagga ataaaaggag ttgaagaatt agggtttgtt tttccctttt
    22561 tgcactctgt taagggagat gagaaagttg ctgttgttga tacaagtata ttaatagatg
    22621 gaagagtata tgaactggca aaaagtggtt ttatagatta tactctcgtt atcccgcaat
    22681 ttgttttaaa agaattacat gaactatcag attgttcaag tgatatgaaa aggcaaagag
    22741 gaagaagggg acttgaaaca attaataaaa tgagaaaaga tgcgtccttg gatgtaaaaa
    22801 tttatgatac tgattatcct gatatacctt ctgtggatgc caaacttgtt aaacttgccg
    22861 ctgatttaag ggctgctgtt ttaacaaatg attttaatct atcaaaactt gcctctgtcc
    22921 agaatataaa aattctgaat tttaatactc ttgctgatat tcttaaacca cgacttgcaa
    22981 gtggtgaaag aatttcatta aagattataa aagaagggaa agaagcagac cagggaattg
    23041 ggtatcttga agatggaaca atgatagtag ttgaaaaagc atctaaatat gttggaaaag
    23101 tggcagatat tattatagat agtgcaatcc agacatcttc tggaagaata atttttgcaa
    23161 aattaaaaac ataatgtcaa ttgctataat agttgcaggg ggaaaagggg aaagaacagg
    23221 aaagaatatc ccaaaggcat ttttggaaat ttcaggtaaa ccacttcttt tttattcaat
    23281 agaaaaattt cagtcctttt cttctttaat aattgttctt ccaaaaagcc atctggaaat
    23341 gtggaaagat aagttaaaat taaaatatcc tgatataaat atggaaatag tccctggtgg
    23401 agaaaatagg caggcatctg tttggaatgg gattgaaaga ataaaagagg atgaagaaat
    23461 tgttttaata catgatgttg ccagaccttt attatcaaaa aatttaataa aaagagtttt
    23521 aactggcact gaaaaatatg gggcatgtgt tcctgttatt aaatgtgtag atacattaaa
    23581 aaaaataaaa aataaaaaaa tagaaaaaac acttgataga aatgaggttt ttcttgttca
    23641 gaccccacag ggatttaaag caggcataat aaaacaggca tatgaaaaag caaagaagga
    23701 aaattttatt gggactgatg attcaatgtt ggtagaaaga ataggattac ctgtatattg
    23761 tattgaggga gaaaggacaa atttaaaaat tacctatgaa gaagatatag gtatagtaga
    23821 atcaatttta aatggagttt taaaataaat ttagagatag taattttgat ttgaatttcc
    23881 ctttttaaaa tttttcaatt tcatcttcat ttaatacttt gatgttgttt ttttccaata
    23941 ttttttctcc attttcagga ttttcaactc taaaaactac aattgctctt tcttctgatt
    24001 tttcaacaaa agcatacata tattctacat ttattccatt atcaacaaaa attttcagaa
    24061 tatgagatag tccacctggt tcgtccttaa cttttgcagc aataactttt gtctctttta
    24121 ctgagatatt atttcctttc aatattatgt ttgcctgttg cctcttatta acaataattc
    24181 ttaaaatccc ataatttgct ttatcagcca gagaaagagc tctaatatta atattattct
    24241 ctccaagtaa agagacaact tcaaacaacc ttcccaatct attttcaaga aatattgata
    24301 attgagttat catttttccc ccctatattt ttctcaggtc aataattctg cttgctttgc
    24361 ctgtacttct ctcaattgat tttggttcaa caagatttac ttcaacatca atgccaatta
    24421 cactttcaat ttcttttttt actttttttt caagttcttc aagttttttt acttcatcag
    24481 aaaacgtctc ttctgtaatt tcaacattta tttgtaattt atctaaatat ttttctcttg
    24541 taacaactaa ctgataatgt ggagcaactt ctggaatatt taaaaggact gtttctacct
    24601 gtgatgggaa aacatttact ccttttatta ttaacatatc gtctattctt cctttaatct
    24661 tacttattct tgggaaagtt cttccacagg cactacattt ctgataattt atggaaacaa
    24721 tatcaccagt tctatacctt attaaaggta atgcttcttt tgtcagagtt gttattacaa
    24781 gttctccttc ttttccctct tcaagggtat tttctgtgtt aatatctatt atttctggaa
    24841 gaaaataatc agaccagata tgaagtccac attttccctc acattcctgt gcaataccag
    24901 gtccaattat ttccgataat ccataaatgt ccagtgcagt tataccccat attttttcaa
    24961 tctcttttct cattctttca gaccacggtt cagcaccgaa aacaccaatt ctaattggta
    25021 tttttgatgt ctcaattcca attgattttg cttcttctgc catatataaa gcatatgaag
    25081 gagttcctgt taaaacagta cttttaaaat cttgcataat catcaattgt cttttagtcc
    25141 ctcctgcaga tgttggaatt acactggctc caatttttaa tgctccataa tgaatgccaa
    25201 gccctcctgt aaaaagtcca taaccaaaag cattctgaat aatatcttcc ttcccaactc
    25261 ctgcacatac taaacttctt gccataactt cactccataa ttttatatca ttttttgtat
    25321 aaccaacaac tgtcaatttt cctgttgttc cagaagaagc atgaatttca taaacctctg
    25381 aaaggtcaac agcaaaaagt ccaaaaggat aattttctct caggtctttt tttactgtaa
    25441 atggtatatt ttttagatca tcaagagaag ataatttatg aatattaatt ttgttttttt
    25501 caaaaaggtt attataaaaa ggtacttttt tattgacata atctaaaatt tttttaagac
    25561 gatctaactg aatttgtttt aatttacttt cagaaatagt ttctattccc ttattaaaat
    25621 aagccatatg ccctccattg gattaaatta tttgtaatat atttttaaag ttttcaagta
    25681 ttaactattt taaaattgta aaaaaatttg tcaacgatat ttttatgtta aaatattttc
    25741 ttaatggtaa tacaatggaa aaaaaatatt tacttggaaa tgaagcactt gcttatggtc
    25801 tttttgaagg aggacttgag gcctcttttg cttatcctgg gaccccatct tctgaaataa
    25861 cagaaaaatt agttgaaata agcaaagagg aaaatattta tattgaatgg agtataaatg
    25921 aaaaagtatc ttatgaaaat gcttatggtg taagtttaac agggcgaaga agttgtgtaa
    25981 ttatgaaaca tgttggttta aatgttgctt cagacccttt tataacatct gtttataccg
    26041 gtataattgg ggggctggta ataattgttg cagatgaccc ctttgcttat tcttctcaaa
    26101 atgagcaaga tagtagaaga tactggcaat tggcaaaaac agtttgtttt gagccatcat
    26161 caattcaaga gagtaaaaat atagcaaaat tttcttttga attttctgaa aaaattggac
    26221 ttccagttat gattagaagt gttacaaagt tatcccatgg taaatcagat gttttgattg
    26281 ggaaaagaca aaaaaaaatt agtgattatg gatttaaaaa aaatccttca aaatttgtaa
    26341 tggttccttc aaatgcaaga gtagcactaa aaaatttgaa tgagaaacag gaaataataa
    26401 aaaaagaagt tgagaaattg cctttcagta aatttgagaa aggagaaagt aatataggta
    26461 ttgttgcctg tggacttact tatagttatg taaaagaatt tgtagaaaca gagaaaaaaa
    26521 attccccaat ttttaaggta tcaacatatc ctttacctga aaaaaaatta aaaaatttct
    26581 tagaaagtgt taatgaaatt gtcgtttttg aagaaggaga cccggttgct gaagaacaga
    26641 tttatattta ttctaaaatt tataatttaa aaaataaaat ttatgggaaa ataaataatt
    26701 ttactgataa agaaggtgaa ctatcagttg ataaagttga agagacattt tataaaataa
    26761 gagggaaaaa gaaaaaatat tattcttttt ctattccaga aagaccgcca gttttatgtg
    26821 cagggtgccc tcatttaggt tctttttata ttctaaaaaa ggtttttggg aaagatgcct
    26881 tgtttccagg agatattggg tgttatacac ttggtgttaa attagaaaca attgatacat
    26941 gtctttgtat gggagcaggt atttctatgg gcagtggtat gtcaagattt gaaaaaaata
    27001 atgtagtatc aattattggg gattcaacat tttttcatac aggaattccg gcccttataa
    27061 atgcagttta taatcaagca aatcaagtta tatgtattct tgataatagg acaactgcaa
    27121 tgacaggtca tcaaccacat ccaggaacag gaataaatgc taagaaagaa aaaacagttg
    27181 aaattgaact taaaaaatta atatctaatt gtggagttga agatgttatt gaagttgacc
    27241 catataaaat aaaagaagca atagaaaaaa taaagggaat aaaagataaa aagggagtaa
    27301 aagtaataat ttttaagaga gaatgtataa ttacatcaaa aggaaagaaa atccaatatt
    27361 atattgactc aaaaagatgt aagggatgca aattgtgttt aactattaac tgtcttgcca
    27421 tgagttttaa aaatggcaaa gcagaaattg gaattttatg taatggatgt ggaatgtgtg
    27481 aaaatatttg tccttataag gcaatagtca aagatgaaaa aaagggttga tattttgatt
    27541 tgtggagttg gaggacaagg aatacttctt acctcaaata taattggtat tgcaggtgta
    27601 gaagaaggat tttctgtcag aggtactgaa attcatggaa tgtcacaaag aggaggttca
    27661 gtagaggcac atatacgatt gaattgtaat tatggtcctc aaatacctta tgggaaggga
    27721 gatattttaa ttggatttga accacttgaa gtagcaagat attctaatta tcttaaagaa
    27781 aatggaattt gtgtagttaa tagttataaa attcctataa ttgggtatga atatgatgaa
    27841 aaagaaataa ttaaaattat aaaaagaaaa acagataaaa tttatattga gaattttact
    27901 gaaaagtcaa aagaattagg ttatgttaga tgtttgaata tattaatgct tggttttgct
    27961 tcaaatttta tacctataaa agaggaatct tttataaatg caataaatat gtatgtaaaa
    28021 aaagaatttg tcaaaattaa ttatcttttt tttaaatctg gaagggaatt atatcattta
    28081 taaagtgaat tattcccaga aaaaaaggtt attatcagaa atttttttag accaatcaat
    28141 aacaagtgtt ttactacctg ctggaattct cacaatactg cctttttgga aatcatctat
    28201 attttcattt ttgaagtcaa aaatattttt tttgcacata cacataaatg cttcaagtcg
    28261 tgttgaagtt ggctgtaagt catgcccacc attttcataa tattttacaa agacattttt
    28321 ccctaatttt tttaattctt ttacgagttc ttcagtatgt tcattatgtg gaactacatg
    28381 gtcgtttgta ccatgttcta aaaaaacagg gcattttatt aaattagcat gctccattac
    28441 atctcttata gaaatttcat aatcaccaaa atatcttcct gcccaatcaa tatatgtatt
    28501 tgttaacttt actattggag aagaagcata tacaaatgta aatgtatcag gagcaaaaat
    28561 tgaacttaaa aggcatatat gtccaccctg actacctcca taatgataaa ttctttttct
    28621 atttatatcc cttatgctta aaaaaaacct taaaccattt aaaacatcaa atacctgaaa
    28681 aaaacttgca tcataaggaa catatgaacc aagacctgtt atagaattaa aatcaaatcc
    28741 actctgtcta tattctgttg aaatagaaat tacattgaaa tttgttgtag catattccat
    28801 tctatcaacc ggctgaaatc tatttccacc ccagccgtga gtaaaaaaca taattcctgt
    28861 ttcagaattt attttatctg gctcaattac taaaagtgtt attggtttta gatatgattc
    28921 ggaatttaag ttttcataag ttatctcgta ctttctcata tattattctc ctgtttaatt
    28981 tttggtttta ttatacttga taaattccat gctatttctt ccataccaga aggtgatgga
    29041 tgaacaaggt caactccaag tccatcaaca tttttcagaa tttcagttcc aggtatataa
    29101 ataacttttt cattatttaa actttcaact gtttttttaa caatttctct aaattttttt
    29161 tcttttccat tgcaatcatc aaaaaaatca ctaccgaatg taaaaatatc aatacaaaaa
    29221 atatatttat caggattttt ttcctttatt gtaggtatga aatattcaac tcttttttta
    29281 aattcttcaa cttcaaaatt atacaacata tttattccca tttcaagagt agcaaagttc
    29341 cacttaactt tttctgctat gtaatcagcc atttcttttt ctaaatgtgc tccaccacca
    29401 aaacccaaat ttataaggtc aacaccaaga agttgtgcag ttctcattac ataacttcct
    29461 gtaggtctta ttgcagattg tccttgcgta attgaagaac cataagataa gtatttaatg
    29521 gaaggaacct gttctttttc tggtatgtca aaattacctt caatatcaac tatttttaaa
    29581 attatagaac gtggcagaat tattcttgtc aaaattgggt caaaaggtag cccatttttt
    29641 tcagtcaaaa tttttaattt tttactatta gataacaggt cttctctttt taaggtaatt
    29701 gttgtaattt catcagaaat ccattgaaac gaatgaaaaa aatttccttg ataaatttca
    29761 taaataccac ctgccccttt atcttttgcc tgaagcataa ttttccctat atcacttttt
    29821 aaattaaacc ttatttcaca tccatcaggc atcaatgccc ttgtttgtaa atattcgttc
    29881 tgttttagat gttttcttaa cacatcggga atccttgaaa ttatgttttt ttctttgcca
    29941 atttcaacta attcataaac attatataac tcaacatttt tgtaaatcat aattttctcc
    30001 tttttaaagc aaatttaaaa atgaaattgt tatattttta ttgaataaac tctttatgta
    30061 cctgttttaa tgctttttct ccgtcttttt ttcttacaat agaagagatt ttaatttcag
    30121 aagtacttat catttctata tttacaccaa cttttgcaat agcagaaaac atccttcctg
    30181 caactcctgg attattcatc attccaattc caattattga tattttacaa atatcagtgt
    30241 ctttttcaac tttttctgca ccaagttttt ctcctaattt ctgggatatt tcaaatgctt
    30301 tttcaaggtc atttatatca actgtaaatg aaatgtcagc atagttttgc ttacttacat
    30361 tttgaacaat catatcaaca tttatatttt catttcctat tgaggtaaaa atttcagcag
    30421 caattccagg tctatcagga attctaaaaa ttgttatttt tgcctggttt ttgtcaattg
    30481 aaacagcaga aacacttgct ccttctttta cttccatttc tttaatcatt gttccttccc
    30541 cctttttgtt aaaagttggt ttcacaacaa atggcacttt atattttttt gcaaattcaa
    30601 ctgctcttgc ctgcattact tttgctcctg accatgccat ttcaagcatt tcatcataag
    30661 atatttcttt tataagtttt gcttcactga ttatatttgg gtctgctgta aaaacacctt
    30721 caacatctgt atatatttca cataattttg cttttattgc cgcagcaatt gcaattgcag
    30781 taaggtcaga cccacctctc ccaagtgttg ttattgtatt ttcaaaactt attccctgaa
    30841 atccagcaac tacaactatt ttatttttat ttaattccct tattatgttt ttggggtcta
    30901 ttttctggat tcttgctttt gtatgaacat tgtctgtttt tatacctgca aaatatcctg
    30961 gaaacccttc tgcttcatac cctaaatttt caatagccat acaaacaaga gatacggata
    31021 ccatttctcc agttgacagc agaaggtcaa gttctcttgc aggagggaaa gtagttatct
    31081 gttttgctaa ggaaattaag ttatctgttg tttttcccat agcagaaaca acaacaacaa
    31141 caccatatcc attttttttt gtttcaacaa ttctttctgc aacaaatttt attttttcaa
    31201 tatctattaa agaactacca ccatatttct gaactattat atccattttt tacctttttt
    31261 aagtaataat tttacactat tttttccttt tacaaaatta tatattggca agcattgttt
    31321 caagaagaaa aaggcaccct cttttatcaa gtggattgtt tttatttcca cattttggtg
    31381 actgaataca ggatggacag ccagattcac atttacatcc ttttacactt tcataagata
    31441 aatttagaat tttttctatt ctttcatatc cagtttttgt aattccaatt cccccaggat
    31501 acccatcata tataaatatt gttgcttttt ctgtttgttg atgaaaaggg aaagaataac
    31561 caccaatatc acctctatca gaagggactt caacaggaaa aattcctata attgaatgtt
    31621 caactgcgtg gatactccca tgaaaatcaa tctttctctt tttaaattct tcaatttgtt
    31681 tttcgtgtat ttcaatccac agagattgtg tttgaaattt tatatctggt aaatttaatt
    31741 gataaaggcc aataagcgtt ttgtcttttt ctttcctttt ttcataactg acaaaattag
    31801 ttgtgacttc aattttacca aatttaattt tgaattcatt tcctttttct tttatcatat
    31861 ccattatatt aattttttcc caccaattaa cctgggtata ataatcaact ttcgctttct
    31921 gaacaattac ctgctttgtt tgtggtttta taaatgaaac ataatattta tttcctaaat
    31981 gtaaataaat tgctcctgga tgacagtcat aataaacttt attttcttca atttctccta
    32041 tgatttttcc tgtatctact tcaactattg aaaaaatatc cccaaccgtt cttaaattaa
    32101 cctgtgtatg tggatatttt ccggagtaaa agtatcttcc atcaaatgtt tttttgaatt
    32161 ttgtttcaag tatttctttt ataaaattac catacaaatt tatttcattt tcactgaaag
    32221 gcatttcaaa acaagcacat ttaagatgat tttcagtaat tatttcattt tcaaaattta
    32281 taattacatc ttcaaattct ctttcaagaa gttcatctgg attttttaaa aaatacttat
    32341 caagagcatc agaaagtgcc acaaaaataa caatggactc attatttttt cttccaactc
    32401 ttcctgctct ctgccaggta cttattatac ttcctggata accaaaaaga atgcaggtat
    32461 ctaaccagcc aatatcaatt cctaattcta aagcacttgt tgatattact ccttttaaat
    32521 tcccttcaaa caaatttttt tctatttctc ttctttcttc tggaagataa ccagccctat
    32581 atgaagaaat tacatttttt aaggatggat ttttaattcc ccatatctgt aaaagttcag
    32641 ttgcccttct gctatttgta aaaacaattg ttgaaagtcc attttcaaca ctttttttca
    32701 tcaattctat tgtttgagta taaggagagt cctcaagtgt atcccagaaa acaaaatgtt
    32761 ttttcccgga aggagcagaa ttctctttta tagattcaaa atcaagtcca acaagttgac
    32821 ctgcaaaact ttctgaattt ttaattgtag cagaagaaag aataaattgt gggttgctcc
    32881 cataaaaatt acaaattctt cttagccgtc ttataacatg agaaacatta gagccaaaaa
    32941 ttcctttgta agtatgaact tcgtcaataa ctacaaaaga aagttcagaa aaaaattccc
    33001 tccataatga ataaaaaggt aaaattccaa tatggagcat atcaggattt gtaagaacaa
    33061 ttgaagggaa ttttcttttg attttttttc ttatattttc aggtgtatca ccatcatata
    33121 tttctgctgt tattgaaggg aatttttttc taaaattttt taatgttttt aattggtctt
    33181 gagttaatgc ttttgttgga aatatataaa gtgcttttga atcaggtgtt ttaagtatat
    33241 ggttaattac aaatgtatta taaataagtg ttttgccact acctgttggt gttgtaatta
    33301 ttatgttttt cttatcattt agtttttcaa gtccttcttt ctggtgaata taaatttttt
    33361 caattccatt ttctttaaga taatttttta tttcaggact tataaaatca ggtatatcat
    33421 atttttcacc ttttttctct ttaatagttt taagataaac aatatgtttc tttaagtcag
    33481 aatttttaag ataatttaga aattttaaga cattattcat ttatttccct ccagaccagg
    33541 tcaggcagac caggcccagt ggaattttaa tttgtgaatt ttgtaagagg ataatgaaat
    33601 ccctttaaaa attcagatgt ttcaattctt tttttacctt ccatctgcaa ttgctttatt
    33661 ttaatagttc catttccaca agcaacttca ataaagtcat ttccaatttt aattatttct
    33721 cctatcatac caaatttacc actttctttt tctatttctg ttttaaaaat tttaattaac
    33781 tttttcccat tttttgtttc aatatatgtg taagcggttg gccattcaat aactcctcta
    33841 acaaggttgt gtatatcttt tgcacttttt tcccagttaa ttttaccatc ttcttttttt
    33901 aatttccttg cataagaagg aattcctttt tgtttttcac ctctctctcc cttttttatt
    33961 ttttctatag cagaaattaa aattttgcta ccttcatttg aaagacgatt ttttaaagta
    34021 attgcatcat catcaggaag aattgggaat tttctctgtt ctattatttc ccctgcatct
    34081 atgttttctt ccattttaat aacagttatt cctgtttctt cctccccatt tattaaacac
    34141 cattcaatag gagcaggtcc tctatatttt ggaagaagtg atggatgtaa atttatagaa
    34201 cacaattttg gtatttttaa gatttctact ggtaattttt tcccataaga aataagaaca
    34261 attaggtctg gatttaatga ttttaattta gaaataaaat tttcatcaaa ttctgctggt
    34321 gttaaaactt caattttatt ttctatcgcc catatttttg taggagttgg tagaactttt
    34381 tgtcctcttc cttttggttt atcaggagtt gttataatag tcaataaatt aaaatttttt
    34441 ttcaattcat aaagagatgg tattccaaat tcgtcacttc caaaataaat tattttcatt
    34501 ttgtaaaaag taaagaggca gaagaaataa taccagagtc aattccaaga cctgctggaa
    34561 caacttttac attttcactg agttttttca ttgctctttc ttttattgtt tttcttattg
    34621 tttcaaaaag aatatcccct gcctgtgaaa ttcctcctcc tatgactata atatctggat
    34681 taagaagatt aataagagaa gagaataatg ctcctaaaca aattccaaca tcagtccata
    34741 tatcctttgc aacttcatca cctaaattat atgcatcaga aattattttt ggcgttattt
    34801 tatttaagtt tccaccagca agttcatata tttttgtttc tttcccttct tttattgcat
    34861 ttactgctct ttcaacaata taatctctac caacaaatct ttctatacat ccataatttc
    34921 cacatttaca cttttgtcct ttgtaatcaa ttgtgatatg tcctatctca actgctgaat
    34981 atttttctcc tcttaaaagt tttccatctt taacaattcc tcctccaacc cctgttccaa
    35041 gtgtaatgca tatcatggtt ttataacctt taccagcacc aaaaagatat tctccccatg
    35101 caatagtatt aacatcattt tcaatgacta cctttatatc atattttttt tcgaaaatat
    35161 cttttaaagg taaattatcc cagccaggta aatttggtgc tccataaaca atcccttcat
    35221 catcaacaag cccaggtgtt cctatgccaa ttcctattat ttcattttta ttttctccat
    35281 caaggcaaaa gttgattgca ttttcaatct gtgaaaggac aatttctctt ttaccttcct
    35341 cacctcttgt tgggaattga tttttcttta taatatttcc cttatcatca acaagccctg
    35401 cctttataaa agttcctcct aaatcaattc caacacattt cattttttct cctctttctt
    35461 tttaaatagt gaaaagccct ttttttcttt tttttcttct ggtgattgaa cttctttttt
    35521 cttttcttcc tttttccgga aaagtcctct tttttctttt tttaagggct gacttttctc
    35581 cataggttct tttataggta ttttttcttc tttttctgaa atttcctcat attcagaata
    35641 tacaccatat tttttaacaa gttcaattat atttgagtat tgctggaaac ttacagtttt
    35701 tgggactgaa tggtctgaat gataaatata tccaccccct tttttagcaa cttcaaattt
    35761 tttctttatt tcctcttcaa tttttgttag gtcagggtct tccatcaatc ttacatcaat
    35821 tccacccata aaagaaagtt gagccccaaa tttttctttt agttcaatta ggtccattcc
    35881 tgcttttact tccaacggtt gtagacaatc aagtccaaca tctataagaa ttggaattaa
    35941 ttcttttaca tttccgcaac tatgaagaaa aactttcata ccttttgaat gaaaatattc
    36001 atatacaagt ttatctgcct ggtaatgtgt tttcatatat gtttcaggag aaaataaagg
    36061 tccattttta tatcccatat cattataaag aaatgctcca tcaaacttaa atccattttt
    36121 catcataagt tcagccataa tgattgtaag ttctgctaat gtcattatca tctcttttac
    36181 ccaatcaggg ccttctgcca ttgaaattaa aagttgttca ctttttatgt aattttgtaa
    36241 aatatcatat ccatatccac cagaaaaaca gcaaaatttc ccttcttcgt atgctgacac
    36301 attacgagat aaaccacttg cccagtcaac tcttgtaaaa tcaggagtaa gccgtttttt
    36361 tatttctttc cagtcctcct tttttgttat gggccaatca ataacttcag gtgttgttga
    36421 ataatccctg tgattttttc ttttacctcc ataaggagta gttgaaatta tgtattcttc
    36481 acttctttca ataactttta ccggaaattt aggagttgtg tctgcaccaa aaaattctaa
    36541 ttcatatcca aaatattcag atggggtcat atcttctggc aaaccttctt tacgccatct
    36601 tcttatagtt gcatcccaga tactgtcatg aataggaact ctatcaggta ttttatgatt
    36661 taatgtcctt aaaatccttt ctctacttat catttttcct cctttttatt ttaatttttc
    36721 ttttattatt atcatctaaa atttcaattt ttacaagtat taacttttca ggtaaatttt
    36781 ttttaatttt ttcaacccat tcaattaaaa taattccttt tccaatatat tcttcccaat
    36841 ttatttcttc aatttctctt tcgttttcaa ggcgataaag gtcaatatga aacacaggta
    36901 ttttcccata atattcgttt attaaaacaa aagaaggact tattgcctgt aagttacata
    36961 attttttaac aatccctttt ataaaagttg tctttccact tcccaattca cctgaaaaac
    37021 caacaatatc tccttttttc aaacttttag caaatttttc tcctaatttt tctgtttcgt
    37081 ttccagaatt tgtataaaaa attcccattt gttacctttt tgtatctaat gtcctattaa
    37141 tattgcccat ttttttaaat ctgatatctg aacaaatttt cccatttttt cttctgaaat
    37201 aggtagtatt gtgccatttg aattttctgg agaagacaat ttgatatttt taatattttt
    37261 ctcgtttata aaaattttta aattttttat ttcctctttt ttataattta ttaagtgtat
    37321 aataaaagta ttatttgttt tataaccaat atttaaaacc tgtggttctg tctttagctc
    37381 aaagaacgat ttaacatctc tgttaaaaaa taatttttca taatttttaa taaaa
//
LOCUS       DAOOVW010000014        33991 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_852_length_33991_cov_7.711015, whole genome shotgun sequence.
ACCESSION   DAOOVW010000014 DAOOVW010000000
VERSION     DAOOVW010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 33991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33991
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_852_length_33991_cov_7.711015"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(332..1642)
                     /locus_tag="PLW95_03180"
     CDS             complement(332..1642)
                     /locus_tag="PLW95_03180"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HOV21668.1"
                     /translation="MKVLMINPPIYDFSAYDLWMKPIGFLKIADLLVKNGIDVFYFDF
                     LDRNNNFYKEKGIADRKYGTGKFYSIKIEKPEIYKHIPREYKRFGLPDYLFFDFIKTI
                     KKIDFIFITTGMTYWYLGVKEIVEFCKNYFPSVPIIIGGIYATFCFNHAKEKLNVDMV
                     FKGKSIEDFIDQVNNKFSKKIKLFDNIYPFWDFYEKLKYVCIKTSYGCPFSCWYCGIK
                     QLEPSFYQRDHFDVFFELKKNYEKFHFKDVAFYDDALFFNFETHLLKFLELVKKEKIN
                     VSFHTPNGIHPKFITKDVAVILKESNFKTIRLSLETISVEREKESSFKVSFSDFEKAI
                     ENLVIGGYSKEEIGVYILAGLPFQKYEEVIKTIKLLKKYPCKINIAEYSPIPGTIDFE
                     ISKNLYPCLPIDEPLFQNNSIFPLWLSENKWEKIEKLKEFAHSE"
     gene            complement(1674..2492)
                     /locus_tag="PLW95_03185"
     CDS             complement(1674..2492)
                     /locus_tag="PLW95_03185"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOV21669.1"
                     /translation="MEKKLRILVIMAHPDDSDINCGGLALKYTQKGHIVKFVSTTNGG
                     TGHYKIGGIELIRRRYEEAQNSAKVAKLYEYQILDWHTGELEPIVEKRKVIIKIIRDF
                     IPDLIITHRPYDYHPDHRYTSQLVQDASYILTVPNMLPLTEYLKEQPVICYSYDGFKK
                     PLLFQADIVIGIDDVIDKKLEMLHCHTSQMYEWLPFNEGKLDEIPKNEKDRKKYLFNT
                     RFSLYKEIANKYREKLVELYGEKGKVIKYAEAFEISEYGKQIEKEEIFKLFPFF"
     gene            complement(2552..3571)
                     /gene="purM"
                     /locus_tag="PLW95_03190"
     CDS             complement(2552..3571)
                     /gene="purM"
                     /locus_tag="PLW95_03190"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOV21670.1"
                     /translation="MSEKYKKAGVDEVKAEELISYIKKKTEKLHKKKYMIGDIGFFSG
                     FFKFPENFNQPVIVSSTDGVGTKILVSQIMDNYSTIGIDLVGMNVNDVSVSGAKVLFL
                     LDYIAVGELKGKREKQIIDGIVDGCKMADCVLVGGEIAQMKDVYGKNGFDLAAFCVGV
                     VEKNKIVDGSKIKKGDVIIGISSNGVHSNGFSLLRKVFKNSDYKKHYKEIGKKLGDEL
                     LKPTYIYSPLLTKLADNKMIKGVAHITGGGIKGNLVRLIRDNLKCVINKNKWEVPPIF
                     DIIKKEGKISESEMFDVFNMGIGLILVVSPDNVDNALNIIKKYKFSSFVIGEVKEGER
                     EVVLL"
     gene            complement(3568..4629)
                     /locus_tag="PLW95_03195"
     CDS             complement(3568..4629)
                     /locus_tag="PLW95_03195"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOV21671.1"
                     /translation="MNQLEIFQKTINHERHDEFLFYAAFTPYLLEKLCKKYKIEQEEI
                     IDFFGMFNPVFVQPEEGRKEIDFSEYYRDVEIPEGSFINSLGVLEVPAGYYHFTGYIS
                     PLRNVKSISDIENFPFPQAEKFSPANMKEDVEKAHKEGKVSVCWVGHMYETSWQIRGY
                     QEFLMDMIENPEWCEYILDRITERNIKIAELGARAGVDYIKTGDDVANQKSLMFSKEM
                     WRKFIKSRWKKVYEKARSIKPDIQIWYHSDGNIEEIIPELIDIGVTILNPVQPECLDI
                     YKIKREYGKYIVFDGTIGTQSTMPFGKDEDVKSVVRERKEKLGYDGALIISPTHILEP
                     EVPIENIEAFIEECKRTEK"
     gene            4781..5854
                     /locus_tag="PLW95_03200"
     CDS             4781..5854
                     /locus_tag="PLW95_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOV21672.1"
                     /translation="MKLKENCKYGLAVPTSIGVRITPLNNQPVHTSSLYIMQATSAET
                     NVASISSYLGIPVKVLTAFVKGSPIARFIKENLRSRGMDFEGNEFEQAGPWGYRHQFN
                     IGDTGYGARGPKVWNDRSGEVGRILRPEDFDLEGLFGQEGVKIVHMSGLIAALSPDTK
                     KFCLEIANIAKKHNTRISFDLNYRASFWKNREQELRSAFTEIASISDILVGNEEDFQL
                     CLGIKGPEVGGKNIKDQIDSFKEMIERTKKLFPKVEVFATTLREVISANQHLWGAIMS
                     VGKNWYVVEPREIFVLDRIGGGDAFVGGLLYGILKNWTEEKWIQFGWASGALVVTLLT
                     DYAQPLNEEEIWDIWAGKARVKR"
     gene            6059..6817
                     /locus_tag="PLW95_03205"
     CDS             6059..6817
                     /locus_tag="PLW95_03205"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOV21673.1"
                     /translation="MKRNILVIGAHIDDCEGGSCAGISLKFIKLGYKVYFLNTIGNLK
                     SWSIVKDKKTEEALIQEAYKAAEILGTHKILLKFQHNHLFDMDFNVIKEIAKVIKKIN
                     PEIILIPWPKDNHPDHVRTAKASLEAISYFNINSSLGGKTSIKVNLKEILAYEISSWQ
                     TENFKPDFFINITNEMEIVCQSMKAFKHIGTKLYIKDKKAQCSLWGIQAEFKYAEGFK
                     HLGPYFPINSILLALFGKDLRPAGFLQYPWGIRL"
     gene            7566..7868
                     /gene="groES"
                     /locus_tag="PLW95_03210"
     CDS             7566..7868
                     /gene="groES"
                     /locus_tag="PLW95_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOV21674.1"
                     /translation="MAKLRIRPLGDRVVIEPSEAEEKTKSGIILPDTAKEKPQEGKVV
                     AAGKGKTDESGKIIPMEVKVGDKVLYGKYAGTEITIDEKEYIILREEDILAVVEDK"
     gene            7882..9507
                     /gene="groL"
                     /locus_tag="PLW95_03215"
     CDS             7882..9507
                     /gene="groL"
                     /locus_tag="PLW95_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888904.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOV21675.1"
                     /translation="MAKQILLGEEARRVILEGMEIMVKALKPTLGPKGRCVVLEKKFG
                     SPTVINDGVTIAKEIELEDPYKNLGAQLLKEVASKTNDDTGDGTTSASVLGYAIAKEG
                     FKNVAAGANPVHLRRGIEKAVKAIVDKLKLMSKPVKDKHEIQQVATIAAANDIEIGQI
                     IAEAMDKVGKEGVITVEEAKGTETELNVVEGMQFDRGYLSPYFITDPERMECILEDVY
                     ILIYDKKISAVKDILPLLEKVAQAGKPLLIIAEEVEGEALATLVVNKLRGTFQCCAVK
                     APGFGDRRKAMLEDIAILTGGQPILEELGIKLESVDLNMLGRAKRIIVDKENTTIVEG
                     AGSSSKIQGRINQIKSEIEKTTSDYDKEKLQERLAKLAGGVAVINVGAPTETDMKERK
                     ARVEDGLSATRAAVEEGVVPGGGVALLRCQAEIDKLKFDDPDEKTGAKIVQKALESPL
                     RQIAENSGLDGAVVVQKVRESDDVNFGFNAEKDKFEDIVKAGIIDPTKVVRSALENAA
                     SMAALLLTSEALITEKPEKKEEKVPTPPPYPEY"
     gene            9556..9828
                     /locus_tag="PLW95_03220"
     CDS             9556..9828
                     /locus_tag="PLW95_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOV21676.1"
                     /translation="MKRYHLLIGGFVQGVGFRWYCQKIAKKLGLVGWVKNLSDGNVEI
                     LVEGEDSTIKDFLSQLKSGYLGRNISNIEIFEEKYSGEFNDFDIKF"
     gene            9830..10225
                     /gene="queF"
                     /locus_tag="PLW95_03225"
     CDS             9830..10225
                     /gene="queF"
                     /locus_tag="PLW95_03225"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HOV21677.1"
                     /translation="MDEYKDYAKKGLHQKLPDIQYFENQFRKYKIKIIISEFTSICPK
                     TSQPDFGTIIIEYEPDKYVIELKSLKLYIQAYRNLGIFYENAVNRILKDIVKTINPIW
                     IKVTGDFNPRGGIKSVVEVSYSSENNLIK"
     gene            10279..10980
                     /locus_tag="PLW95_03230"
     CDS             10279..10980
                     /locus_tag="PLW95_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21678.1"
                     /translation="MLIDEIEKLVELQKIDSEIKLLENEISLFPEKKKNFQKQLDDKV
                     SALSSIEKESKRVAIERKEKELELKAIEDEIKKLKDRLSEVRTNKEYLAVQKEINEFE
                     KKKENIEEDILVLFEKDDEIKEKEKEFKKQIEELKKEIEIELKEEERRVDDLKKEIEM
                     KKEEREKKINNIEKGLFDLYEKIRKSKKDGVAICRLEDERCAGCSIYVPTYISEKVKR
                     KKEIVQCENCSRILY"
     gene            10980..11396
                     /locus_tag="PLW95_03235"
     CDS             10980..11396
                     /locus_tag="PLW95_03235"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HOV21679.1"
                     /translation="MKKLTVFIDGASRGNPGNASIGIVIYENGNLLMEKGEYIGVGTN
                     NIAEYMALIYSLIECIKLKAKNIEIKSDSLLLVKQIKGEYKVKNEFIKKLNIIANKLI
                     ENFNTFEIKYIPREENKKADKIASEILSYNSLFPSS"
     gene            11418..11761
                     /gene="rnpB"
                     /locus_tag="PLW95_03240"
     ncRNA           11418..11761
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLW95_03240"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            11790..13013
                     /locus_tag="PLW95_03245"
     CDS             11790..13013
                     /locus_tag="PLW95_03245"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF017903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HOV21680.1"
                     /translation="MNEKWIENGIEHLINVFGKEKVERAIEYVKNFDVEIPSWIFGPF
                     GGGRFGEYTPPAAARNIYEKIDDACFVYKITGCGKKVMTHVLWDFSDDGIEPDINIVE
                     KVTNYLKDKEMKLGIVNPTYFLSGSHKGTFTNPEKKVRERYIKQTIFASDIAKKFGNS
                     IISLWFPDGSLYPGQIELDENYKLMKKSFEEIYNKISEDVFILIEYKLFEPGAYSTTI
                     PDWGTSYILAKLRKKHSGVLIDMGHHPYGVNIEQIVATLISDGMRCGFHFNTRYAADD
                     DLAVEPNPQMARIFYYLVKGKVVSNKEENKNWFYGIDQASGIENRIEAVIHSIDSLQI
                     SLAKACLVDINKLNTLQLEDDIIGANRYFNDILINTDVRPIIYKARIEKGLAFDPILE
                     YKKSGYQEKIEKERE"
     gene            13149..13583
                     /gene="mraZ"
                     /locus_tag="PLW95_03250"
     CDS             13149..13583
                     /gene="mraZ"
                     /locus_tag="PLW95_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018664086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOV21681.1"
                     /translation="MVYFGEFRSKLDKEGRIVIPSKMREQLKDRNVYITRGFEECLFL
                     FSETIWNSQTEKLKTLPFTKGDPRAFTRLFFSGAYRTKIDKQGRLNIPLTLTNYAKIN
                     NGVVIIGVGFRIEIWDEKKWDEYYKKFLPSYAEISEKLIGSE"
     gene            13585..14451
                     /gene="rsmH"
                     /locus_tag="PLW95_03255"
     CDS             13585..14451
                     /gene="rsmH"
                     /locus_tag="PLW95_03255"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOV21682.1"
                     /translation="MEHYPVMKEKVLELLNPKEGGIYVDATSGYGGHSKYILKNSAKN
                     IFLYCIDKDDDAIENLKRNLIEFKNFKAIKGGFENLIEILKNENVKKIDGIIFDLGFS
                     IHQIKNPERGFSFSVDGPIDMRYDIEQNLNAKEILNKWAAKDLIYIFKNYGEIKNAER
                     IVSKIIERRKKIEFETTREFADFISSITHRERKKIHPATKFFMALRIATNNEFPNLKD
                     GLNQAVNLLKENGRIIVITFHSLEDRIVKRFLKNRDDIKIITKKVIIPDEEEIRKNPE
                     SRSAKLRAGEKK"
     gene            14448..14783
                     /locus_tag="PLW95_03260"
     CDS             14448..14783
                     /locus_tag="PLW95_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21683.1"
                     /translation="MRKKKFFIPDYFFLIICITPLLIFYVSIHLKVIQAGYQLEKLEK
                     EYDELEFINKTYKAKILELTNPDNLKQLSNNLGLNFISPEKWCYVDIKKKNEENEGVI
                     DNAYAETRQ"
     gene            14758..16524
                     /locus_tag="PLW95_03265"
     CDS             14758..16524
                     /locus_tag="PLW95_03265"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOV21684.1"
                     /translation="MHMQKQDNKNNFNLRMKYGKYFFLFLFSVVLFGLFKIQVIDYSI
                     YSLKDKKTRYEKVKIPVCRGTIYDRNGKILAMSVPVYSVGIDTWVINNLKEKNLDDIE
                     EKKICDCLGIEKQILKEKTKKPYAILKDNLTVEEYQKIKDEYEKGEIKGIYFVRNYKR
                     VYPNGSHCCHILGFIGKDGNGLEGIELFYDNLLRGREGVVLYLKDGKGNLIPTINKVL
                     IPPEEGKDIYLTVDSNIQFMVEEEIKDGLKNFNSNSISEIVMDPENGEILAMANVPDY
                     DLNFPSKFPESYRRNRCITDFLEPGSVFKIVTGTAAIEEGIFSPYNIIFCENGKYFVR
                     GHYVHDTHKYGDLSFIDVIVKSSNIGTIKIALSLGEEKLYEYCKKFGFGQLTEIDLPG
                     EIKGVLRPLEKWSNYSITAVPIGQEVGITSIQGIRAMAVLSNGGYLVWPHLVKKVTLP
                     DGKDFFKVPIKKRKVISERSCEIMKGALLKVVSEQGTAPLASIEGYKIYGKTGTAQKA
                     ENGRYVHGKYCASFIGFLTQNNSNIIISVNVDEPKPLYYGGVVAAPIFKNTMWRILQY
                     CDVPPDNNHEEIKIVLEKKGKK"
     gene            16521..18020
                     /locus_tag="PLW95_03270"
     CDS             16521..18020
                     /locus_tag="PLW95_03270"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOV21685.1"
                     /translation="MKLKDLIKDIDKKVYNFKNIDIKGIAFDSRKVRDGYIFVALKGE
                     KEDGHKFIPEAIERGAKCIVVEKKTSFLISDVVEIVVDDTKKVLAIISSRFYNDPSSK
                     LKIVGITGTNGKTTTSFLIKNILEKAGEKAGLIGTISYQIGQRQIISFNTTPESADLQ
                     AFFSEMLKEECKWAVLEISSHGIEQRRILGINFDCGIFTNITPFEHLDYHKTFKNYLQ
                     TKLKFFSIYLKESKKENKKAIINLDDKFSSYFLREAKKAKIESIITYGKHRKSDIKLI
                     DYSLTRNGNKIIFQRYGEKIEVFTHLRGIGNVYNCLAAISFATGYNIDKEIIIKGIEE
                     TLSVPGRFEFIDEGQPFDIIVDYAHTHNALLTLLQSVRQMKPKRIILVFGCGGNRDKS
                     KRPLMGKIGVKLSDIVILTSDNPRSEEPREIIKDIEKGIPFYLKKKYAVIIDRKQAIK
                     EAISLAGENDCVVIAGKGHETFQILKDVTVPFDDREEAKKTVKELYGKN"
     gene            18007..19362
                     /gene="murF"
                     /locus_tag="PLW95_03275"
     CDS             18007..19362
                     /gene="murF"
                     /locus_tag="PLW95_03275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOV21686.1"
                     /translation="MEKIEIEKIVKWCKGEINNKNFLPLYINGISTDTRKIKKGEIFI
                     ALKGEKFDGHQFVQEAYKNGAIGAIVDKNISFSSEKKFFIIRVKDTLKSLGDIAKEYR
                     NYINPYIIGITGSDGKTTTKELIAKCLAEKFNVRANQGNYNNQIGLPLSLLSFDKKTE
                     IGVLEMGMSKKGEIDYLSKIAKPDSCAITNIGRAHIGFFKNIKEIAESKSEILNNLKG
                     EKFCLFNRDDNFFNFLKSRSSGNVKSFGKSIKADVRGIIIDEGNDFFSFKVNNFNEKF
                     IVNFWNTSMIYPSLISIAFSIKFGIDFDKIKGVIEKFNSIEGRGKVYKIKGVCLIDES
                     YNSNPNSLKNSLICFSRKNFKRKIAIIGDMAELGKFSSFYHKYIGQLIRKLDIDMVIS
                     LGEKSKIISDLNLGKHFSDIEKLNLFIKNKIKTGDGILIKGSNFLNMGEITKFLIKEI
                     G"
     gene            19366..20442
                     /gene="mraY"
                     /locus_tag="PLW95_03280"
     CDS             19366..20442
                     /gene="mraY"
                     /locus_tag="PLW95_03280"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOV21687.1"
                     /translation="MLYKLLYECVKYWFGFNVFKYITVRATLATFTSLIISIIFGKKF
                     INSMKRFCHPNDFLNFRENNKKDIPTMGGIFILSTLLFSTILWADISNIYILLLIFLT
                     IYLALFGYWDDMKKLVNQSSRKGLRPIIKIVAQVGAGLVIGIVLVYGQGINFDTSVYL
                     PFFKKVVIPLGEYYILFAIFLIVATSNAVNLTDGMDGLAIGSVMIVTLAYAAISYLAG
                     NIKFAEYLSIPYVKGAEEVTIFTGALVGSCLGFLWYNCYPAEIFMGDTGSLPLGGIIG
                     FLAIIVKQELSLIFVGGIFLIEGLSVVIQIISVKTRGKKVFLMTPLHHHFEKRGIPES
                     KVVIRFLILTLILSLFTVITLKIR"
     gene            20439..21773
                     /gene="murD"
                     /locus_tag="PLW95_03285"
     CDS             20439..21773
                     /gene="murD"
                     /locus_tag="PLW95_03285"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOV21688.1"
                     /translation="MNLENKKVVIVGFGKTGLAVANFLLNKKVNVFITDKEATKEKIE
                     NLKKLNGKIEVEFGKHSSSFLKGTDLIVISPGVDERVLPEIVLEEKIPVVSEIELSYS
                     FSPSKKIIAITGTNGKTTTTLMAGNLLKFAYQPYVVCGNIGNPFISEIEKITKDTYII
                     IEVSSFQLEKIRNFKPYIGVILNIADDHFDRYANFNEYIRSKEKIFINKEENDWAVLN
                     GDDKNCINIGKKIKGNKIFFGFSKNFDVYFKNNSIYYNNEKIVNFDKTNLIGKGNIYN
                     TMAIVSIGKICNIKNEIIEESLVNFTPPSHRMEKVRQINGITFINDSKATNPHAVENA
                     LECLPDGKVILLMGGLDKELPFKQLRNIIEKKVKLLVLFGKAKDKIENELKIKKIPVR
                     KFDNLEDAVDFSYKNSEKGDIVLLSPGCASFDQFKNYEERGNVYKRKVSNLL"
     gene            21739..22869
                     /locus_tag="PLW95_03290"
     CDS             21739..22869
                     /locus_tag="PLW95_03290"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HOV21689.1"
                     /translation="MFIRGKSAIYYRICISSAFLIVCGTVFLLSSSVSYGISEGGNPL
                     LYLYERIIWIILSVFLLYVLKKIGIEKIEKISFLIFIFSLALLTIPVMLNLIYHKPEY
                     LRWIKIGPLSFQPSELVKLTFIIFLANYLNMRDKMINNWKVLLFPVLFFLFIIIILQF
                     QKDMGTVVIMGATFFFLLFLAGLKMKYITIFSILGVILIAGLILLFPYRIERIKNFLN
                     PTSDPKGGGYQIIQSRITLGSGGVFGKGLGRGEGKLSFLPVVNKDYIYAVVGEEKGFI
                     GTSFILILFAILVFSSFEVSTLAKNNFDKYLSAGIGTLFGFQFLLHALVVISIIPSKG
                     TTLPFFSKGGSSFVINFLTLGILIIIAKRVCIKGDLDDFLDF"
     gene            complement(22895..23713)
                     /locus_tag="PLW95_03295"
     CDS             complement(22895..23713)
                     /locus_tag="PLW95_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21690.1"
                     /translation="MNKIKIIFFIIFLSFPYFLLSETLNYVFKEDVPVSYKFKIAGDI
                     SFGYNQTEKNNFKVLATGNIDIKTIKTNNSYTLEIIPRKTLIKVNDMVIEDITKNETE
                     ISNVVSTSRVEMQKNGKIIKTEEIEPNIINIGNFLSLLPVFPEGKMYKGRTWQQKISS
                     FEIPGFLKMCNLDFKYFISDIKEERYTIKLISNNSIIDEKEVDKDITIEFKGTNNSNG
                     NFVFNGNKGKLEKFNGNFKLNLKLFFLLPGTPGENTTNQSLPMDLKINLSISLE"
     gene            complement(23706..24848)
                     /locus_tag="PLW95_03300"
     CDS             complement(23706..24848)
                     /locus_tag="PLW95_03300"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOV21691.1"
                     /translation="MISKRAEEIKPSVTLSINQMAKMLISEGKDVVNLSVGEPDFDTP
                     QPIKEFAKKVIDEGFTKYTEDKGIKELREVIAEKLLKENNVKYMPDQIVVSNGAKHSL
                     FNIFLAILNPGDEVIIPSPYWVSYPAMVKIAGGVPVFCKWNEKFKVDIDELKQKITPR
                     TKGIILNTPSNPTGIIYTKEELEEISEILVRNNIMCISDEVYEKIIYDKIHISIASIS
                     DEMKKLTIVVNGVSKTFAMTGWRIGYIAGEKEIIDAVSKIQSQTTSAPSSISQKAAYF
                     AIKEGEYLYKNMVEEFKKRRNFLVENLSKKIIFPYPDGAFYLFCSFNNMDSKIIAQKL
                     LEEKFVSVVPGDDFGAEKFFRISYATSIKNLEKAVERINEFVNENE"
     gene            complement(24845..26716)
                     /locus_tag="PLW95_03305"
     CDS             complement(24845..26716)
                     /locus_tag="PLW95_03305"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004068215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HOV21692.1"
                     /translation="MDNNLKAKLNEKNYEKLSNVKNKKVLDFISYFVSLCNPDSVFVR
                     SDSEEDIEYIRNKAIEYGEEIKLKKDGHTVHFDGYYDQGRDKGNTKLLVTKGMELGSL
                     LNYTDREKGYEEIMQLLKNIMVGKEMYVLFLCLCPVNSPFSIYSIQLTDSAYVAHSED
                     ILYRPAYSVFTEKEDIDFFKYVHSAGELTEDKTSKNFDKKRIYVDFLEDTVYSVNTQY
                     AGNTVGLKKLALRLAIRKAYKEKWLAEHMFIMGVKGKGRKSYFLGAFPSMCGKTSTCM
                     VKGETIIGDDIAYLKKRNGNVYAANVERGIFGIIQDVNEKDDPLIWKSLMTPGEVIFS
                     NVLLSNGNVFWNGCGLEIPDEGINFSGKWWKGKKDEEGEEIPPSHPNARYTIRLNSLE
                     NFDPEGENKEGVEVSGIIFGGRDSDTWPPVFESFDWQHGVITIGGTLESETTAATLGK
                     SGVRVFNIMANMDFISIPIGKYLENYLEFGKKDIVKIPKIFGVNYFLKDENGNFLTDK
                     QAKRVWLKWMELRVHNEGKIIKTPIGYIPEYNDLKFLFKDVLSQEYTENDYLAQFSLR
                     VSNNISKIDRVIEIYKGLKDIPEIVFDVLENQKEQLIDLKNKYGDVVSPYHIREENI"
     gene            27349..27846
                     /locus_tag="PLW95_03310"
     CDS             27349..27846
                     /locus_tag="PLW95_03310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HOV21693.1"
                     /translation="MKKIIAVCGSNEGDENLNSDVLEIAEEVGFLIAKNGGILICGGL
                     GGVMEASAKGAKKGGGITVGIMPWKKDKANKYIDIPIETNLGFYRNSIIVNSADSVIG
                     ICGRWGTLNEISMAIALGKPTILISTSGGICELFSDKEVLKNFKTKPKIAKSPQEAVN
                     LAFSL"
     gene            complement(27848..29503)
                     /locus_tag="PLW95_03315"
     CDS             complement(27848..29503)
                     /locus_tag="PLW95_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOV21694.1"
                     /translation="MKKTIYIFNFIFLILFLIYPLSYIISQSFIVDNKFTLEILKASI
                     GNYILRTSLIKSFSLGIIVVFLTSIIGIPLAFFFYKYNFKAKNFLRIAFFLPLIATPF
                     VGALGIRQILSRFGSLNLILIKYGILKNPISWIGTGFAGIVILQTLHLYPIMFLNIST
                     TLSNFDIECEEASYNLGANFLQTFRKITFPLFLPGYFAASSIIFIWSLTDLGTPLVFD
                     YTNLISIQVFNHINDINTNPVGYALVLIITFITLLLFVLTKKYIEKTPYVSGRIKRLE
                     SGKNLDRKGKVFYFLTILLLLFSLTPHVGIILNSFAKKWFFTIFPSEYTGEFYKTVFT
                     HHLTTTGIFNSLFLSSSAALIDAVLGFTIGYFLARENFKGKQLLDLLVMLPIVIPGII
                     IAFGYFACFSNTIIDPRHNPYFLLIISYSIRRLPFIVRSTFSSFQLIGEEMEQASYSL
                     GATNNTTFKKITLPLVSPGLFAGCIFVFAFSFMEVSSSLILAIREKFYPVAKVIYTLA
                     GRVTDGPYVACALGVLGMIIISVCIVISSKITKVRFGEFFKFG"
     gene            complement(29500..30552)
                     /locus_tag="PLW95_03320"
     CDS             complement(29500..30552)
                     /locus_tag="PLW95_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV21695.1"
                     /translation="MNEILVVKNIEKSFGGNKVLKDINFSVEKGELFFILGSSGCGKT
                     TLLRIITGFEVADKGKILLENTDITNLHPSKRKIGMVFQNYALWPHMTVYHNIAYGLE
                     IRNRGKEFIDKKVDEIIKITKLENVKDLYPYQISGGQQQRVALARALVIEPKVLLLDE
                     PLSNLDTKLRESLRREIKRIQKETSTTMIYVTHDQKEAMSLATRIAIMDEGKIVQIDT
                     PLNIYLNPINRSVASFIGEVNFIDGKVKEEKAKEIFVETEEGDFVVSNFSNFKRGDNI
                     TLGFRPEFISFSGKENLIEGKITDFEYLGEITRVSLKTKKGNNFIICVLVENFTDLQI
                     GEKISFSVKKFMVFEK"
     gene            complement(30558..31880)
                     /locus_tag="PLW95_03325"
     CDS             complement(30558..31880)
                     /locus_tag="PLW95_03325"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOV21696.1"
                     /translation="MKSKYKKYIRIFLLFFMIISFDLIGDEKLVIISPHWEGVQIEVS
                     DAFEKWYLKNYGEKVKIEWIDQGGTSDDLKFVESLFKKNPTGIEIDIFFGGGMDPYLR
                     LKELGYLEKYKVPSQILKKIPQMCAGVPNYDKDFYWYGVVLSGFGILYNKKVLEYFSL
                     PIPKTWKDLGRKEYFSLVGAADPRHSGSMHMMYEIILQAYGWKEGWETILAIAGNTKT
                     FSSSAAQIAKDTSIGEVAVSLCIDSYALSQIEINGEENLGFFFPENLTVINPDAIGIL
                     KGAPNMEVAKKFINFLLSYEGQLLWMLEKGKEFGPKKYSLNRFSILPDVYNHPDLKNK
                     YINPYKSGQTIKYNFILATKRWDVLNDMIGCFAIDCHPYLKKAWKIVKDSENNSLKRI
                     FFQIPFEEEEQKLLWFNWNNSIFRNQYINEWLNFSIKKYKGIIKEKRI"
     gene            complement(32174..32980)
                     /locus_tag="PLW95_03330"
     CDS             complement(32174..32980)
                     /locus_tag="PLW95_03330"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOV21697.1"
                     /translation="MKIVKFLKNNKENYGIYKGDFIEVIKWQPFIDDFEETGERVNIE
                     EIKFLPPVFPPNIIALGLNYKEHAQESKMDLPEEPVIFLKATTSITGHLSPIFLPSVA
                     PDYVDYEAELCIIIGKRAKNVSEKEVRNYIFGYTCGNDVSARDCQFKRDKQWARGKSF
                     DTFCPIGPWIETEVNPDNLRIQSRVNGKVMQDSNSSYMIFSPFEVVSYLSKQFTLLPG
                     TVIMTGTPSGVGYAREQPVFLRDGDIVEVEIEGIGILKNYVKSEIGNYVE"
     gene            33281..33354
                     /locus_tag="PLW95_03335"
     tRNA            33281..33354
                     /locus_tag="PLW95_03335"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33315..33317,aa:Arg,seq:tct)
     gene            complement(33468..33890)
                     /locus_tag="PLW95_03340"
     CDS             complement(33468..33890)
                     /locus_tag="PLW95_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21698.1"
                     /translation="MKGEKSFSGFIVGFIGVSFIISSLVFASPQKTTIKEILDNPDKY
                     DKQEVIVEGKVEKIKLTVSKKDNPYTTFNLIDDDYNGIKVFIWEHQEIKNKDIKNNDK
                     VEVIGTFQKVKYVGKYRFYNEIEAESIKKLKAEESEKE"
BASE COUNT        12465 a         4936 c         5037 g        11553 t
ORIGIN      
        1 aataatgtaa tcagtattta tatcatcacc aaatttatga acatatcctt ttagtatcat
       61 atcatctcct ttctttttta gaaaattaaa ttccccttgc cccagttgtg ctatctggga
      121 gacgataacg caccaactct gaggcacaag gtaagcaact tcagtcggtt ttacaaaggg
      181 gaaacgtttg tctccttcct ttgaaaaagg aaggttagga tggattttct tttgtgtcct
      241 tgtcctaact taacataacg ggcttcactg aagtatctat ttaaaaatta ccatattttt
      301 atatatttga caatctttat gaaaattatc tttattccga atgggcaaat tctttaagtt
      361 tttctatttt ttcccattta ttctcagaca gccataaagg aaaaatagaa ttattttgaa
      421 ataatggctc atcaataggt aaacagggat ataaattttt acttatctca aaatcaattg
      481 ttcctggtat tggtgagtac tctgctatat ttattttaca gggatatttt tttaataatt
      541 ttattgtctt aataacctct tcatatttct gaaagggcag acctgcaaga atataaacac
      601 caatttcttc ttttgaatat ccccctatta ccagattttc aattgctttc tcaaaatcag
      661 aaaaagatac tttaaagctt gactcttttt ccctttcaac acttattgtt tcaagagaaa
      721 ggcgaattgt tttaaaattt gattctttta aaatcactgc cacatctttt gttatgaatt
      781 ttggatggat accatttgga gtatgaaaac ttacatttat tttttccttt tttaccagtt
      841 ccagaaactt caaaagatgg gtttcaaaat taaaaaaaag tgcatcatca taaaaagcaa
      901 catctttaaa atgaaatttc tcataatttt ttttcaactc aaaaaaaaca tcaaaatggt
      961 ctctctgata aaaagaaggt tcaagttgct taataccaca ataccagcaa gaaaaaggac
     1021 aaccataaga tgttttgata cagacatatt tcaacttctc ataaaaatcc cagaaaggat
     1081 aaatattgtc aaaaagttta attttttttg aaaatttatt gttgacctgg tctataaaat
     1141 cctcaatact tttcccttta aaaaccatat caacatttaa tttttccttt gcatgattga
     1201 aacaaaaagt tgcatatatc ccaccaataa taataggaac agatggaaaa taatttttac
     1261 aaaactctac aatctctttt acccctaaat accaatatgt catacctgtt gttataaaaa
     1321 taaagtcaat tttttttatt gtttttataa aatcaaaaaa taagtaatcc ggtagaccaa
     1381 atcttttata ttctcttggg atgtgtttat aaatttctgg cttttcaatt ttaattgaat
     1441 aaaattttcc agtcccatat tttctatccg caattccttt ctctttataa aaattgttat
     1501 ttctatcaag aaaatcaaaa taaaaaacat caataccatt tttaacaagc aaatcagcaa
     1561 tttttaaaaa gccaatgggt ttcatccaca aatcataagc agaaaaatca taaattggtg
     1621 ggtttatcat aagaactttc attttatctc gtaccaatta gcacattgat aggttaaaaa
     1681 aatggaaata acttaaaaat ttcttctttt tctatttgtt ttccatattc agatatttca
     1741 aatgcctcag catattttat tactttccct ttttctccat ataactcaac aagtttctct
     1801 ctatatttat ttgcaatttc tttgtaaaga gaaaatcttg tattaaaaag atattttttt
     1861 ctatcttttt catttttggg aatttcatct aatttccctt cattaaatgg aagccactca
     1921 tacatttgag aagtatgaca atggagcatt tccaattttt tatcaataac atcatctatt
     1981 ccaataacaa tatctgcctg aaataaaagg ggttttttaa aaccatcata tgaataacaa
     2041 attactggct gttcctttaa atattcagtc agtggcaaca tatttggaac tgttaatata
     2101 taagaagcat cctgaactaa ttgtgaagta tatctatggt ctggatgata atcataaggt
     2161 ctatgagtaa ttattaaatc aggaataaaa tctctgataa ttttaattat tacctttctt
     2221 ttttcaacta ttggttcaag ttcaccagtg tgccagtcaa gaatttgata ttcatataat
     2281 tttgcaactt ttgcagaatt ctgtgcttct tcatatcttc ttcttattag ttctattcca
     2341 ccaattttat aatgtcctgt tccaccattt gttgttgaca caaattttac aatatgacct
     2401 ttttgtgtat atttcaaagc cagtccacca caattaatgt cagaatcatc tggatgtgcc
     2461 attattacca aaattcttaa ttttttttcc atcctctttt cttctccttt tttcaatctg
     2521 gaaaaaataa tttatttttt tagaaattaa atcaaagtaa aactacttct ctttcacctt
     2581 ccttaacttc tccaataaca aaagaggaaa atttatattt tttaattata tttaaagcat
     2641 tatcaacatt gtcaggtgaa acaactaata taagtccaat tcccatattg aaaacatcaa
     2701 acatctcact ttcagaaatt tttccttctt tttttataat atcaaaaata ggaggaactt
     2761 cccatttgtt tttatttata acacatttta aattatctct tatcaatctt accaaatttc
     2821 cctttatccc tcctcctgta atatgagcaa caccttttat catcttgtta tctgcaagtt
     2881 ttgtcaaaag tggtgaataa atatatgttg gctttaaaag ttcgtctcct aatttcttcc
     2941 caatttcttt gtaatgcttt ttgtagtcag aatttttaaa aacttttctc aaaagagaaa
     3001 aaccattgct atgaactccg tttgaagaaa taccgataat tacatctcct ttttttattt
     3061 tacttccatc aacaatttta tttttctcaa caacaccaac acaaaaagca gcaaggtcaa
     3121 acccattttt cccataaaca tctttcattt gtgcaatttc ccccccaact aatacacaat
     3181 ctgccatttt acaaccatca actatcccat caataatttg tttttctctt tttcctttta
     3241 actcaccaac tgcaatgtaa tcaagaagaa agagaacttt tgcacctgaa acactaacat
     3301 catttacatt cattccaaca aggtcaattc caatagttga ataattatcc ataatttgag
     3361 aaactaaaat ttttgttcca actccatctg ttgaagaaac aataactggc tgattaaaat
     3421 tttcaggaaa tttgaaaaaa ccggaaaaaa aaccaatatc accaatcatg tatttttttt
     3481 tatgtaactt ttccgttttc ttttttatat aagaaattaa ttcttctgct tttacctcat
     3541 ccacccctgc ttttttatat ttttcgctca tttttcagtt cttttacatt cctcaataaa
     3601 tgcctcaata ttttctattg ggacttctgg ttcaagaata tgcgttggag aaatgattaa
     3661 agcaccatca tatcccaatt tttcttttct ttctcttaca acacttttaa catcttcatc
     3721 ttttccaaat ggcatagttg attgagtccc tatggttcca tcaaaaacaa tatatttacc
     3781 atactctctt ttaattttat aaatatctaa acattctggc tgaacaggat ttagaattgt
     3841 aactccaata tcaattagtt caggaataat ttcttcaata tttccatcac tgtgatacca
     3901 tatttgaata tctggtttta tactccttgc tttttcatat acctttttcc atcttgattt
     3961 tataaatttc ctccacatct ctttagaaaa cataagcgat ttttgatttg caacatcatc
     4021 accagttttt atataatcca cccctgctct tgctcctaat tctgcaattt taatattcct
     4081 ttctgttatt ctatcaagta tatattcaca ccattctggg ttttctatca tatccatcaa
     4141 aaattcctga tagcccctta tctgccacga tgtctcatac atatgcccaa cccagcaaac
     4201 agaaactttt ccttctttat gtgctttttc tacatcttct ttcatatttg ctggactaaa
     4261 cttttctgcc tgcgggaatg gaaaattttc aatatcacta attgatttga catttcttag
     4321 tgggcttata tatccagtaa aatggtaata tcctgctgga acttcaagaa cacctaacga
     4381 atttataaaa cttccctcag gtatttcaac atccctgtaa tattcagaaa aatcaatttc
     4441 ttttcttccc tcttctggtt gaacaaaaac aggattgaac atcccaaaaa aatctattat
     4501 ttcttcctgt tctattttat attttttaca taatttctcc aaaagataag gagtaaaagc
     4561 agcataaaaa agaaattcat cgtgtctttc gtggtttatt gtcttttgaa atatctctaa
     4621 ttgattcatt ttttattttt accaataatt taatttttac atttttttat tttcttataa
     4681 tttaatccta tttctttcta ttgtcaaact atttaatttg ccatttatgt taaaataaat
     4741 tataatttca aaattaaaaa ggattaatat ggaggagaaa atgaaattaa aagaaaattg
     4801 taaatatggt cttgcagttc caacaagcat tggagttaga ataacccctt taaataatca
     4861 accagttcat acaagttctt tatatataat gcaggcaacg agtgctgaaa caaatgttgc
     4921 aagtatttct tcttatttag gaatacctgt aaaagttctt actgcttttg ttaaaggaag
     4981 cccaattgca agatttataa aagaaaattt gagaagtaga gggatggatt ttgaaggtaa
     5041 tgaatttgag caggcaggac catggggata tagacatcag tttaatattg gagatactgg
     5101 atatggagca agagggccaa aagtatggaa tgatagaagt ggtgaagtag ggagaatttt
     5161 aagacctgag gactttgact tagaaggact ttttgggcaa gaaggagtta aaattgttca
     5221 tatgtcaggg ttaattgcag cactttctcc tgatacaaaa aaattttgtt tagaaattgc
     5281 caatatcgca aaaaaacata acacaagaat ttcgtttgac ctaaattaca gagcatcttt
     5341 ctggaaaaat agagaacaag agttaagaag tgcctttact gaaattgcaa gtatttcaga
     5401 tatacttgta ggaaatgaag aggactttca actttgttta ggaattaaag gaccagaagt
     5461 tggagggaaa aatataaaag accagataga tagttttaaa gaaatgattg aaagaacaaa
     5521 aaagttattt cctaaggtag aagtctttgc tacaacttta agagaagtaa taagtgcaaa
     5581 tcaacattta tggggtgcaa taatgtcagt tgggaaaaac tggtatgtag tagaaccaag
     5641 agagattttt gttcttgata gaattggtgg tggtgatgct tttgttggtg gacttttata
     5701 tggaattctc aagaactgga cagaagaaaa atggatacag tttggatggg catctggagc
     5761 acttgttgta acacttttaa ctgactatgc tcaaccacta aatgaagaag aaatatggga
     5821 tatatgggca ggtaaagcac gagtaaaaag gtaaaaaatc taatcacaat ttgcaggaat
     5881 atttttctct tgaatttggg ggacagaaat actatgatga aaaagaagga attataaaaa
     5941 gtatagagaa tcaaaaaatc tcccaaaata gcaggaaatg atattatttt tctccttatt
     6001 ttgaaatact ataaataaca taagaaaaat tattaaaaat ttttatggag gaaaaacaat
     6061 gaaaagaaat atattagtta ttggagcgca tatagatgac tgtgaaggtg gaagttgtgc
     6121 aggaataagt ttgaagttca taaaattagg gtacaaggtt tattttctta atacaatagg
     6181 caatcttaaa agttggtcaa ttgttaaaga taaaaaaact gaagaagctt taattcaaga
     6241 agcgtataag gcagcagaaa ttttgggtac tcataagatt ttgttaaaat ttcaacataa
     6301 tcaccttttt gatatggatt tcaatgtaat aaaagaaatt gcaaaagtaa ttaagaaaat
     6361 aaatcctgaa attattttaa ttccatggcc taaagacaat catcctgacc atgttagaac
     6421 agcaaaagca tcattagaag caatttctta ttttaatatc aattcttcac ttggagggaa
     6481 aacgtctatt aaagtaaatt taaaagaaat attggcatat gaaataagta gttggcaaac
     6541 agagaatttt aaacctgatt tttttataaa tattactaat gaaatggaaa tagtatgtca
     6601 atctatgaaa gcatttaaac atattggaac taaactttat ataaaggata aaaaagcaca
     6661 gtgtagtcta tggggaattc aagcagaatt caaatatgct gaaggattta aacatcttgg
     6721 tccatatttt ccgataaatt caatattact cgccttattt ggtaaagatt taagaccagc
     6781 aggttttctt caatatccat ggggaattcg actctaattt gcatattttt aacctaaaat
     6841 ctcgtttttt tatcactatg tattatattc aggtataggt aaatcaaatt ttttcaaaat
     6901 atctttttgt tttttagaaa tttcagtcaa aaatatttta ctattttcca tctctactat
     6961 tttgattttt ttaattcata aagaagttct ataagagtat aatctttaaa aatacctttt
     7021 tctctcataa ttttacaaac acctgaatat agaataagag aaaggaaact caaagattgt
     7081 ctcgcttgtt tcttctcttt atcccaataa ctttctcctt catatacata aacattcttt
     7141 cctatttttt gttctattct atataactat ttaaaattta aatggcaact gctttaattc
     7201 cttatttttc catagatttt atcagataag aaaaacatag ttattaatat cgctggattt
     7261 taggtttaga gaaaaagttt tagtaagtag tggtcttgaa cacttagggc tgacagaaat
     7321 tactgataaa cttatggata ttccagctaa aatccaacac aaaaatattt gtacaataat
     7381 acttcttcct ttatatcata atttcagcag aatatttgtt gaaaataaaa ttcccaacaa
     7441 taaaatactt tatccctttg atacttctta aaatcatttc ctgcaaaatg ttgacaaata
     7501 tttacactta ggtataatta ttatctaaaa aaataactct atacttttaa aaaaggaggt
     7561 gaaatatggc taaattaaga attagaccac ttggagatag ggttgtaatt gaaccatcag
     7621 aagcagaaga aaaaacaaaa agcggaataa ttttacctga tactgcaaag gaaaaaccac
     7681 aggaaggaaa agttgttgct gctggaaaag ggaaaactga tgagagtggt aaaataattc
     7741 caatggaagt taaggttgga gataaagttt tatatggaaa atatgcagga actgaaataa
     7801 caatagacga aaaagagtat atcattctta gagaagaaga tatacttgca gtcgttgaag
     7861 acaaataaaa ggaggtgaag tatggcaaaa caaatattac ttggtgaaga agcaagaagg
     7921 gttatacttg aaggaatgga gataatggta aaggcactaa aaccaactct tggaccaaaa
     7981 ggaagatgcg ttgtattaga aaagaagttt ggttctccaa cagtaattaa tgatggagtt
     8041 accattgcaa aagaaattga acttgaggac ccatataaaa atttgggagc ccaactttta
     8101 aaagaagttg cttctaaaac aaacgatgat actggtgatg gaacaacttc tgcatcagtt
     8161 ttaggatatg ctatagcaaa agagggtttt aaaaatgttg ctgctggtgc aaatcctgtc
     8221 catttaagaa gaggaataga aaaagcagta aaagcaattg ttgacaaatt gaaattaatg
     8281 agtaagccag tgaaagataa gcatgaaatt caacaggttg caacaattgc tgctgcaaac
     8341 gatattgaaa tagggcagat tattgcagaa gcaatggata aagttggtaa agaaggagtt
     8401 ataacagttg aagaagcaaa agggacagaa acagaattaa atgtggttga aggaatgcag
     8461 tttgatagag gatatctttc tccatatttc attaccgacc ctgaaagaat ggaatgtatt
     8521 cttgaagatg tttacatttt aatatatgat aaaaaaatat cagcagtaaa agatattctt
     8581 ccacttcttg aaaaagttgc acaggcagga aaaccacttt taataattgc agaagaagtt
     8641 gagggagaag cacttgcaac tcttgttgtt aacaaattaa gaggaacatt ccagtgttgt
     8701 gctgtaaaag caccaggttt tggtgataga agaaaagcaa tgcttgaaga cattgctatt
     8761 cttacaggcg gtcaaccaat attagaagaa ttgggaatta aattagaaag tgttgactta
     8821 aacatgcttg gaagagcaaa aagaataata gtagataaag aaaatacaac aattgttgaa
     8881 ggagctggct cttcatcaaa aatacaagga agaataaatc agattaaaag tgagattgaa
     8941 aaaacaacat ctgattatga caaagaaaaa ttacaggagc gacttgcgaa attagcaggt
     9001 ggtgttgctg taataaatgt cggagcacca actgaaacag atatgaaaga aagaaaagca
     9061 agagttgaag atggattaag tgcaacaaga gcagcagttg aagaaggcgt tgttcctggt
     9121 ggtggtgttg ctttattaag atgccaggca gagatagata aactaaaatt tgatgaccct
     9181 gatgagaaaa caggagcaaa aattgttcaa aaagcacttg aatcaccatt gagacaaatt
     9241 gctgaaaatt caggacttga tggggcagta gttgttcaaa aagtcagaga atcagatgat
     9301 gtgaattttg gattcaatgc cgaaaaagat aaatttgaag atatagtgaa agcaggaatt
     9361 attgacccaa caaaggttgt caggagcgct ttggaaaacg cagcaagtat ggctgcatta
     9421 cttcttacat cagaagcact tataacagaa aaaccagaga agaaagaaga aaaagttcca
     9481 acaccgcccc cttatcctga atactaataa gtaaatagtg aaggaggtcc cttttgggga
     9541 cctccttcta taaaaatgaa gaggtatcac cttttaattg gcggttttgt tcaaggtgta
     9601 ggttttagat ggtattgtca gaaaatagca aaaaagttag gacttgtcgg ctgggtaaaa
     9661 aatttaagtg atgggaatgt tgaaatatta gttgaaggag aagatagcac aataaaagat
     9721 ttcttatctc aattaaaaag cggttattta ggtagaaata tatctaatat tgaaatattt
     9781 gaagaaaaat atagcggtga atttaatgat tttgatataa aattttaaaa tggatgaata
     9841 taaagattat gctaaaaaag gacttcacca gaaattacct gatattcagt attttgaaaa
     9901 ccaatttaga aaatacaaga taaaaataat aatttcagaa ttcacttcta tatgtccaaa
     9961 aaccagccaa ccagattttg ggactataat aattgaatat gagccagata agtatgttat
    10021 tgaattaaaa tcattaaaat tatatattca agcatataga aatttaggta ttttttatga
    10081 aaacgctgtg aatagaattc tgaaagatat tgttaaaaca attaatccca tatggattaa
    10141 ggtaactggt gatttcaacc caagaggtgg aataaaatct gtcgttgagg ttagttattc
    10201 ttctgaaaat aatttaataa aataattttt ttcataatta ttttgatttt ttaaaaatct
    10261 attttataat tataaattat gttaatagat gaaattgaaa aattagttga actacagaaa
    10321 attgatagtg aaataaagtt gttagaaaat gaaatatctc ttttccctga aaagaagaaa
    10381 aattttcaga aacagttaga tgataaagta tctgctcttt catcaattga gaaagaatct
    10441 aaaagagtgg caattgagcg aaaagagaaa gaacttgaat taaaggcaat agaagacgag
    10501 ataaaaaaat taaaagatag attgagtgaa gtaagaacaa ataaagaata tttagcagtt
    10561 caaaaagaaa ttaatgagtt tgagaaaaaa aaggagaata tagaagaaga tatactggtt
    10621 ctttttgaga aagatgacga gataaaagag aaagaaaaag aatttaaaaa acagatagaa
    10681 gaacttaaaa aagaaattga aatagagcta aaagaggaag aaagaagagt cgatgactta
    10741 aaaaaagaaa ttgaaatgaa aaaagaagaa agagagaaaa aaataaataa tattgaaaaa
    10801 gggttatttg acctttatga aaaaatacga aaaagcaaaa aagatggtgt agcaatatgt
    10861 agacttgaag atgaaagatg cgctggttgt tcaatttatg ttcctaccta tatcagtgaa
    10921 aaagtaaaaa gaaaaaaaga aattgttcag tgtgaaaact gtagtagaat tctttactaa
    10981 tgaagaaact tactgttttt attgatggtg caagtcgtgg aaatccagga aatgcttcta
    11041 ttggaattgt aatatatgaa aatggaaact tacttatgga gaaaggtgaa tatataggag
    11101 taggaaccaa caatattgct gaatatatgg ctttaatata tagtttaatt gaatgtataa
    11161 aattaaaagc aaaaaatatt gaaattaaaa gtgatagttt attacttgtt aaacagatta
    11221 aaggagagta taaagtaaaa aatgaattta ttaaaaaact aaatataatt gcaaataaat
    11281 taatagaaaa ttttaataca tttgagatta aatacatacc aagagaagaa aataaaaaag
    11341 cagacaaaat cgcttctgaa attttatctt acaattcact tttcccatcc agttaaaaaa
    11401 ggtataattt taaaaaatag agtaacgggg cagtcgccta tcaattttga taggaggaaa
    11461 gtccggactc cagcaaggaa gatggctcct aacagaagca gttcacaagg actggaaagt
    11521 gtcacagaaa ctataccgtc cgcaatatta taagcggata agggtgaaat ggcgaggtaa
    11581 gagctcaccg tctatctggt aacagataga gcaggacaaa ccccatctgg agtaaggtca
    11641 agcagggctt cggttccttg ccctaaaagg cccgggtaga ctgcttgagt caacaagtaa
    11701 ttgttgacca agatgaatga ctgccttaaa acagaatccg gcttaccagt tactctattt
    11761 ttttcttata agttaaagtg aggaaaatta tgaatgaaaa atggattgaa aatggtattg
    11821 agcatttgat aaatgtattt ggaaaagaaa aagttgaaag agcaattgaa tatgttaaaa
    11881 attttgatgt tgaaattccg tcatggatat ttggaccttt tggaggaggg agatttggag
    11941 aatatactcc accggcagca gcaagaaata tttatgagaa aatagatgat gcctgctttg
    12001 tatataaaat tactggatgt ggaaaaaaag ttatgacaca tgttctatgg gacttttctg
    12061 atgatggaat agaacctgat ataaatattg ttgaaaaagt tacaaattac ttaaaagata
    12121 aagaaatgaa attaggaatt gtaaatccaa catattttct ttctggttct cataagggaa
    12181 ctttcacaaa tccagaaaaa aaagttagag aaagatatat taaacagaca atttttgctt
    12241 ctgatatagc aaaaaaattt ggaaatagta ttatttcttt atggtttcca gatggctcat
    12301 tatatcctgg ccaaatagaa cttgatgaga attataaatt gatgaaaaaa tcttttgaag
    12361 aaatttacaa taaaatttca gaagatgtat ttattttaat agagtacaaa ctttttgaac
    12421 caggagcata tagtacaaca attcctgatt ggggaacttc gtatatcctt gcaaaattaa
    12481 ggaaaaaaca ctctggtgtt ttaattgata tgggacatca tccttatgga gtaaatattg
    12541 aacagattgt tgctacctta atttcggatg gcatgagatg cggtttccat ttcaatacaa
    12601 gatatgcagc ggatgatgac ctggcagttg aacctaaccc acaaatggca agaatttttt
    12661 actatttagt taaaggaaaa gtagtttcaa acaaagaaga aaataaaaat tggttctatg
    12721 gaattgacca agcatcagga atagaaaata gaattgaggc agtgattcat tcaattgact
    12781 cattacaaat ttctcttgca aaagcatgcc tggtagatat aaataaatta aataccttac
    12841 aattagaaga tgatattatt ggagcaaata gatattttaa cgatatatta ataaacacag
    12901 atgtaagacc aatcatttat aaagcaagaa ttgaaaaagg acttgcattt gacccaattt
    12961 tagaatataa aaaatctggt tatcaggaaa aaatagaaaa agaaagagaa taatttttta
    13021 aaacctacct acccttttta cctttcaatt tttctgctaa aaagcacatc tttcctataa
    13081 tttttaattg atgaaaaaaa atttgacaaa ataaaaaata gggtgttaaa tatataaaaa
    13141 ggtaaaaaat ggtatacttt ggtgaattta ggagtaaatt agataaagaa ggaaggatag
    13201 taattccttc aaaaatgaga gaacagttaa aagatagaaa tgtttatatt acaaggggat
    13261 ttgaagaatg cctcttttta ttttctgaaa caatatggaa ttctcaaaca gaaaaattga
    13321 aaacattacc atttacaaaa ggagacccaa gagcatttac aagattgttt ttttcaggtg
    13381 cttacagaac aaaaattgat aaacaaggac ggctgaatat ccctttaacc cttacaaatt
    13441 atgcaaaaat aaataatgga gttgttatta ttggagttgg tttcagaata gaaatatggg
    13501 acgagaaaaa gtgggatgag tattataaga aatttttacc aagttatgca gaaatttcag
    13561 aaaagttgat tggttcagaa taaaatggaa cattatcctg taatgaaaga aaaagtttta
    13621 gaactattaa acccgaaaga gggtggaatt tatgttgatg caacttcagg gtacggggga
    13681 cacagcaaat atattcttaa aaattcagca aaaaatattt ttctttattg tattgataaa
    13741 gatgatgacg caattgaaaa tttaaaaaga aatttaatag aatttaaaaa ttttaaagca
    13801 ataaaaggtg gttttgaaaa tttaatagaa attttgaaaa atgaaaatgt aaaaaaaatt
    13861 gacgggataa tatttgacct tggtttttca attcatcaga ttaaaaatcc tgaaagagga
    13921 ttttcttttt ctgttgatgg cccaattgat atgagatatg acatagaaca aaatcttaat
    13981 gcaaaagaaa ttttaaacaa atgggctgct aaagacctta tttatatttt taaaaattat
    14041 ggggaaataa aaaacgcaga aagaattgta agtaaaatta tagaaagaag aaaaaaaata
    14101 gaatttgaaa caacgaggga atttgctgat ttcatttcat ctattactca cagagaaaga
    14161 aaaaaaatcc accctgcaac aaaatttttt atggctttga gaattgcaac aaacaatgaa
    14221 ttccccaatt taaaagatgg cttaaatcag gcagtaaatt tactaaaaga aaatggaaga
    14281 attattgtca tcacctttca ttctcttgag gatagaattg taaaaaggtt cttaaaaaat
    14341 agagatgata ttaaaattat aactaaaaaa gtaattattc ctgatgaaga agaaataagg
    14401 aaaaatcctg aaagtagaag tgcaaaatta agagcaggtg agaaaaaatg agaaaaaaga
    14461 aattctttat ccctgactat ttttttctta taatttgtat tactccatta cttatttttt
    14521 atgtatcaat tcaccttaaa gtaattcaag caggttatca acttgaaaaa ttagaaaaag
    14581 aatacgatga acttgaattt attaataaaa catataaggc aaaaatttta gagttaacca
    14641 atcctgataa tttaaaacaa ctttccaata acttaggact taattttatt tcaccagaaa
    14701 aatggtgtta tgttgacatt aagaaaaaaa atgaagaaaa tgagggggta atagataatg
    14761 catatgcaga aacaagacaa taaaaataat tttaatctta gaatgaaata tggaaaatat
    14821 ttttttcttt ttcttttttc tgttgtattg tttggccttt tcaaaattca ggtaattgat
    14881 tattcaattt attcattaaa agacaaaaag acaaggtacg aaaaagtaaa aatccctgtt
    14941 tgtagaggaa caatttatga tagaaatggc aaaattcttg ctatgagtgt acctgtctat
    15001 tctgttggaa ttgatacatg ggttatcaat aatttaaagg aaaaaaattt agatgatatt
    15061 gaagagaaaa aaatatgtga ttgtcttggt atagaaaaac aaattttaaa agagaaaacg
    15121 aaaaagccat atgcaattct aaaagataat ttaacagttg aagaatacca aaaaataaaa
    15181 gatgaatatg aaaaaggaga aataaaggga atttattttg taaggaatta taagagagtt
    15241 tatcctaatg gtagtcattg ttgtcatatc cttggtttca taggaaaaga tggaaatgga
    15301 cttgaaggaa tagaattgtt ttatgataac cttttaagag gaagagaagg agttgtttta
    15361 tatctgaaag atggtaaagg taatttaatt ccaacaataa ataaagttct tattccccca
    15421 gaagaaggaa aagatatata tcttacagta gattcaaata tacagtttat ggtagaagaa
    15481 gaaataaagg atggattaaa gaattttaat tccaacagta tttctgaaat tgttatggac
    15541 cctgaaaatg gagaaatact cgcaatggca aatgttcctg attatgacct gaattttcct
    15601 tcaaagtttc ctgaatcata tagaagaaat agatgtataa ctgatttttt agagcctggt
    15661 tcagttttta aaattgttac aggtaccgct gctattgaag aaggaatttt ttctccctat
    15721 aatataattt tttgtgaaaa tggcaaatat tttgtcagag gacattatgt acacgatact
    15781 cataaatatg gggacctttc attcatagat gtaatagtaa aatcaagtaa tattggcaca
    15841 ataaaaattg ctctttcact cggtgaggaa aaattatatg agtattgtaa aaaattcggt
    15901 tttgggcaac tgactgaaat tgatttacct ggtgaaataa aaggggtctt gcgaccatta
    15961 gaaaaatgga gtaattactc catcactgct gttccaatag gccaggaagt aggaataact
    16021 tcaatccagg gtattagagc aatggctgtt ttaagtaatg gaggatatct tgtctggcca
    16081 catttagtca aaaaagtcac tttacctgat ggaaaagatt tcttcaaagt cccaataaaa
    16141 aaaaggaaag ttatttcaga aagaagttgt gaaataatga aaggagcact actaaaagtt
    16201 gtaagtgaac agggaacagc acctctcgcc tctattgaag ggtataaaat ttatggtaaa
    16261 acaggaacag cacaaaaagc agaaaatggg agatatgttc atggtaaata ctgtgcatct
    16321 tttattggat ttctcactca aaataatagt aatataataa tttcagtaaa tgttgatgag
    16381 ccaaaaccac tttattatgg aggagttgta gcagcgccta ttttcaaaaa tacaatgtgg
    16441 agaattttgc agtactgtga tgttccacct gacaataacc atgaggaaat aaaaatagtg
    16501 ttagaaaaga aaggaaaaaa atgaaactaa aggacttaat taaagatatt gacaaaaaag
    16561 tgtacaattt taaaaacatt gacatcaaag gaattgcttt tgattcaaga aaagtaagag
    16621 atggttatat atttgttgct ttaaaaggag agaaagaaga tggacataaa ttcattcctg
    16681 aggcaataga aagaggagca aaatgtattg ttgttgaaaa aaaaacatct tttcttattt
    16741 cagatgtcgt tgaaatcgta gttgatgata caaaaaaagt tcttgcaatt atatcttcaa
    16801 gattttataa tgacccctcc tctaaattaa aaattgttgg aattacagga acaaatggaa
    16861 aaacaacaac aagttttctt attaaaaata tattagaaaa ggcaggagaa aaagcaggat
    16921 taattggtac aatttcctat caaatagggc aaagacaaat tatctctttt aatacaactc
    16981 cagaatcagc cgacttacaa gcattttttt ctgaaatgtt aaaagaagaa tgtaaatggg
    17041 ctgttttaga aatttcttct catggtatag aacaaagaag aattttagga ataaattttg
    17101 attgtggaat atttacaaat attacacctt ttgaacatct tgattaccat aaaacattta
    17161 aaaattacct tcaaacaaag ttaaagtttt tttctatata tttaaaagaa agtaaaaaag
    17221 aaaataaaaa agcaataata aatttagatg acaaattctc ctcatatttt ttaagagaag
    17281 caaaaaaagc aaaaattgag tcaataataa cctatggaaa acatagaaaa agtgatataa
    17341 aattgattga ttattcttta acaagaaatg ggaataaaat tattttccag cgatatggtg
    17401 aaaaaataga agtttttact catttaagag gaattgggaa tgtttataat tgtttagccg
    17461 caatatcttt tgctacaggt tataatattg ataaagaaat cataataaaa ggaattgaag
    17521 aaactttatc tgttcctgga agatttgaat ttatagatga aggacaacca tttgatatta
    17581 ttgtagatta tgctcatact cataatgctc ttttaacatt acttcaatca gttagacaaa
    17641 tgaaaccaaa aagaattatt cttgtttttg gatgtggagg aaacagggat aaaagcaaaa
    17701 gacctttaat gggaaaaatt ggagtaaaat tatctgatat tgttatttta acttctgata
    17761 atccacgaag tgaagaacca agagaaataa taaaagatat tgaaaaaggt atcccctttt
    17821 atcttaaaaa gaaatatgca gtaattattg atagaaaaca ggcaataaaa gaagcaatat
    17881 ctcttgcggg ggaaaatgat tgtgtcgtta tagcaggaaa aggacatgaa acatttcaaa
    17941 tattaaaaga tgtaacagtt ccatttgacg atagagaaga agcaaaaaaa acagtgaaag
    18001 aactatatgg aaaaaattga aatagaaaaa attgttaaat ggtgtaaagg tgaaataaat
    18061 aataaaaatt ttctccctct ttatataaat ggtatttcca ctgatacaag aaaaataaaa
    18121 aagggtgaaa tttttattgc attaaaagga gaaaaatttg acgggcatca atttgttcaa
    18181 gaagcatata aaaatggagc aataggggca attgttgata aaaatatatc tttttcatca
    18241 gaaaaaaaat tttttattat ccgcgtcaaa gatactttaa agtcattagg agatattgca
    18301 aaggaatata gaaattacat aaacccatat ataattggta taacgggtag tgatgggaaa
    18361 acaacaacaa aagaacttat tgcaaaatgt ttggcagaga aatttaatgt aagggcaaac
    18421 cagggaaatt ataataatca aataggtctt cctctttctt tactttcttt tgataaaaaa
    18481 actgaaattg gtgttcttga aatgggaatg agtaagaaag gagaaataga ttatttaagt
    18541 aaaatagcaa aacctgatag ttgtgccatt actaatattg gacgggcaca tattggattt
    18601 tttaaaaata taaaagaaat tgcagaaagt aaaagcgaga ttttaaataa cttaaaagga
    18661 gaaaaatttt gtctttttaa tagagacgat aattttttta attttttaaa aagcaggtct
    18721 tccggaaatg taaaaagttt tggtaaaagt ataaaagcag atgtaagagg aataattata
    18781 gacgaaggta atgatttttt ttcttttaaa gtaaataact ttaatgaaaa atttatagta
    18841 aatttctgga atacttcaat gatttatcca tctcttatat ctattgcttt ttctattaaa
    18901 tttggtattg attttgataa aataaaaggt gtaattgaaa agtttaattc aatagaaggc
    18961 agaggaaaag tatataaaat aaaaggagtt tgtcttattg acgaatcata taattcaaac
    19021 ccgaactcat taaaaaattc gctcatatgt ttttcaagaa aaaattttaa aagaaagatt
    19081 gctataattg gagatatggc tgaacttgga aaattttctt cattttacca taaatatatc
    19141 gggcaactta ttagaaaact tgatattgac atggtaatat cattagggga gaaaagtaaa
    19201 ataatatctg acctaaattt gggaaaacat ttttcagata ttgaaaaatt aaatttattt
    19261 attaaaaata aaattaaaac aggagatgga attttaataa agggttcaaa cttcttgaat
    19321 atgggagaaa taacaaaatt tttaataaag gaaataggat aaaaaatgct ttataaatta
    19381 ctgtatgaat gcgtaaagta ctggtttgga tttaatgtgt tcaaatatat cacagttaga
    19441 gcaactcttg caacatttac atctcttata atttcaatca tttttggtaa gaaatttata
    19501 aattcaatga aaaggttttg ccatccaaat gattttttaa attttagaga aaataataaa
    19561 aaagacatcc caacaatggg tggaattttt atactttcca ctcttctatt ttcaacaatt
    19621 ttatgggctg atatatcaaa tatctatatt ctccttctga tttttcttac catttatctt
    19681 gctctttttg gatattggga tgacatgaaa aaacttgtaa atcaaagttc aagaaaggga
    19741 ttaagaccaa ttataaaaat agttgcccag gtcggtgcag gattggttat aggtattgtt
    19801 cttgtttatg gtcagggaat aaactttgat acttctgttt atcttccttt ttttaaaaaa
    19861 gtagtaatac ctcttggaga atattatatt ctttttgcaa tattcctcat tgttgcaact
    19921 tcaaatgcag taaatctcac agatggaatg gatggacttg caattggttc tgtaatgatt
    19981 gttacccttg cttatgctgc aatatcctat ttagcaggta atattaaatt cgcagagtat
    20041 ttaagtatac catatgttaa aggtgcggaa gaagttacca tttttactgg tgctcttgtt
    20101 ggctcctgtt taggattttt atggtacaat tgttatccag cagaaatttt tatgggtgat
    20161 actggttctt taccacttgg aggaattatt ggatttttag caataattgt aaaacaggaa
    20221 ctctccttaa tttttgtagg aggtatattt cttattgaag gactttcagt tgttattcaa
    20281 atcatatcag taaaaacaag aggcaaaaag gtttttctta tgacaccatt acaccatcat
    20341 tttgaaaaaa gaggaatacc tgaatcaaaa gttgttataa gattcttgat acttacactc
    20401 attttatcac tttttacagt aataactctc aaaataagat gaatttagaa aataaaaaag
    20461 ttgttattgt tggatttgga aaaacaggac ttgcagttgc gaattttctt ttaaataaaa
    20521 aagtaaatgt atttataaca gataaggaag caacaaaaga aaaaatagag aatttaaaaa
    20581 agttaaatgg gaaaattgaa gttgaatttg gaaaacattc ttcttctttt ttgaaaggaa
    20641 ctgacttaat tgtaataagt cctggtgttg atgaaagggt tttacctgaa attgttttag
    20701 aagaaaaaat ccctgtggtc agtgaaattg aattatctta tagtttttca ccttcaaaaa
    20761 aaataattgc tattacagga acaaatggaa aaacaacaac tactttaatg gctggtaatc
    20821 ttttaaaatt tgcctatcaa ccttatgttg tttgtggaaa tataggaaac ccatttatat
    20881 ctgaaattga aaaaattaca aaagatacat atattattat tgaagttagt agttttcaat
    20941 tagaaaaaat cagaaatttc aaaccatata ttggagtaat tttaaatatt gcagatgacc
    21001 attttgatag atatgcaaat tttaatgaat atattagaag taaagaaaaa atttttataa
    21061 ataaagagga aaacgattgg gctgtgttaa atggagatga taaaaactgt ataaatatcg
    21121 gcaagaaaat taaagggaat aaaatctttt ttggtttttc taaaaatttt gatgtttatt
    21181 ttaaaaataa ctctatatat tacaataatg aaaaaatagt taattttgat aaaacaaacc
    21241 ttataggtaa aggaaacatt tataatacaa tggcaatagt atcaattggt aaaatatgta
    21301 atattaaaaa tgaaattata gaagaaagtt tagttaactt tactcctcca tctcacagaa
    21361 tggaaaaagt tcgtcaaata aatggaataa catttattaa tgattcaaaa gcaacaaacc
    21421 cacatgcagt tgaaaatgcc cttgaatgtc tacctgatgg aaaggtaatt ttgcttatgg
    21481 gcggtcttga taaagaacta ccttttaagc agttgagaaa tataattgaa aaaaaagtca
    21541 aattattagt tctgtttggc aaggcaaaag ataaaataga aaatgaatta aaaattaaga
    21601 aaatcccggt tagaaaattt gataacttag aagatgcagt tgatttttct tataaaaatt
    21661 ctgaaaaagg agatatcgtt ttactttcac ctggttgtgc atcatttgac caatttaaaa
    21721 attacgagga gagaggaaat gtttataaga ggaaagtcag caatttatta tagaatttgc
    21781 atttcatctg cctttttaat tgtttgtggc actgtttttc tattaagttc ctctgtttca
    21841 tatggaattt cagaaggcgg aaatccatta ctgtatttat atgagagaat tatatggata
    21901 attttgagcg tttttcttct atatgtttta aaaaaaattg gtattgaaaa aattgaaaaa
    21961 atttcttttt taatttttat tttttcctta gcactactaa ctataccggt gatgcttaac
    22021 cttatttatc acaaaccaga atatttaaga tggataaaaa taggacccct ttcttttcaa
    22081 ccatctgaac ttgtcaaatt aacatttatt atatttcttg caaattacct caatatgaga
    22141 gataaaatga ttaacaattg gaaggtctta ttatttcctg tcctattttt ccttttcata
    22201 attataattt tacaattcca gaaagatatg ggaacagttg taataatggg agcaactttt
    22261 ttcttccttt tatttctggc agggttaaaa atgaaatata ttacaatttt cagtatttta
    22321 ggagtaattt taatagcagg tcttattttg cttttcccat acagaattga gagaattaaa
    22381 aattttctca atccgacttc tgaccctaag ggtggtgggt accagattat tcaatcaaga
    22441 attactttag gttctggtgg ggtattcggg aaaggtttgg gcaggggaga agggaaactc
    22501 agttttctcc cagttgttaa taaagattac atttatgcag tagttggaga agaaaaagga
    22561 tttattggaa ccagttttat tttaatttta tttgcaattc ttgttttttc ttcatttgaa
    22621 gtttctacat tagcaaaaaa taattttgat aaatatcttt cagcaggaat tgggacactt
    22681 tttggttttc agtttttact gcatgctctt gttgttattt caataatccc atcaaaagga
    22741 acaacattac catttttcag taaaggtggg tcatcttttg ttatcaattt tcttacatta
    22801 gggatattaa taattattgc gaagagagta tgtattaaag gggacttaga tgatttcctt
    22861 gatttttaga aattcaataa tcaagtcctg aaacttactc taaggatata gaaaggttta
    22921 ttttcagatc catcggaagt gattggtttg tagtattttc tccaggtgta cctggcagaa
    22981 gaaaaaataa ttttaaattt aatttaaaat ttccattaaa tttttcaagt ttgcctttat
    23041 taccattaaa aacaaaattt ccatttgaat tgtttgttcc tttaaattca attgttatgt
    23101 ccttatccac ttctttttca tcaataatac tattatttga aattaacttt atcgtatatc
    23161 tttcttcttt tatatcactt ataaagtatt taaaatctaa attacacatt ttcaaaaatc
    23221 ctggtatttc aaaagaagat attttttgtt gccatgttct ccctttatac atcttcccct
    23281 caggaaatac aggaagtaga gataaaaaat ttcctatatt tattatattt ggctcaattt
    23341 cttctgtttt tattattttc ccatttttct gcatttctac cctgcttgtt gaaacgacat
    23401 ttgatatttc tgtttcgttt tttgttatat cttcaattac catatcattt actttaataa
    23461 gtgttttccg cgggataatt tcaagagtat atgagttatt tgtcttaatt gtttttatat
    23521 caatattccc agttgctaaa actttaaaat tattcttttc tgtttgatta taaccaaatg
    23581 aaatatctcc tgcaatttta aatttataac tgacaggtac atcttctttg aagacataat
    23641 ttaatgtttc agataacaaa aaataaggaa aggacaaaaa aataataaaa aatataattt
    23701 taattttatt cattttcatt tacgaactcg ttaattcttt caactgcttt ttctaaattt
    23761 ttaatagaag ttgcataaga aatacggaaa aatttttctg caccaaaatc atctccagga
    23821 acaacagaaa caaatttttc ttcaagtaat ttctgagcaa taatttttga atccatatta
    23881 ttaaaagaac aaaagagata aaaagcacca tcaggatatg gaaaaataat tttctttgat
    23941 aaattttcaa caagaaaatt ccttcttttt ttaaactcct caaccatatt cttataaagg
    24001 tactctcctt ctttaattgc aaaatatgct gctttttgtg aaattgaaga tggcgcagaa
    24061 gttgtttgac tctgtatttt acttactgca tctattattt ctttttcacc tgcaatatat
    24121 ccaattctcc atccagtcat tgcaaatgtt tttgatacac catttacaac aattgtcaat
    24181 tttttcattt catctgatat tgatgcaata cttatatgta ttttatcata aattattttt
    24241 tcgtaaactt catctgaaat acacataata ttatttctaa caaggatttc tgaaatttct
    24301 tccaactctt cttttgtata aataatacca gtaggatttg aaggagtgtt taatattata
    24361 ccttttgttc ttggggtaat cttttgcttc aattcatcta tatctacttt aaatttctca
    24421 ttccatttac aaaaaacagg tactccacct gcaattttta ccatagcagg ataactcacc
    24481 caataaggag atggaattat aacttcatca cctggattta aaattgccag aaatatattg
    24541 aaaagtgaat gttttgctcc atttgaaaca acaatctggt caggcatata ttttacatta
    24601 ttctctttta aaagtttttc agcaataact tctcttaatt ccttaatccc tttatcttct
    24661 gtatattttg taaacccttc atctattact ttttttgcaa attcctttat tggttgtgga
    24721 gtatcaaagt ccggctctcc aactgaaaga ttaacaacat ccttcccttc tgaaattaac
    24781 atttttgcca tttgatttat tgaaagagtt accgatggtt ttatttcttc tgccctttta
    24841 gatatcatat attttcctct ctgatatgat atggagagac aacatcccca tatttatttt
    24901 ttaaatcaat tagttgttct ttttgatttt ccagaacatc aaatacaatt tctggaatat
    24961 cttttaaccc tttataaatc tcaattaccc tatcaatttt tgatatatta tttgaaaccc
    25021 ttaaagaaaa ttgtgctaag taatcatttt cagtatattc ctgtgataag acatctttga
    25081 aaagaaattt aaggtcatta tattcaggaa tatatccaat aggggttttt attatttttc
    25141 cttcattatg cacccttaat tccatccatt ttagccagac cctttttgcc tgtttatcag
    25201 ttaaaaaatt tccattttca tctttaagaa aatagttcac tccaaaaatt tttggtattt
    25261 taacaatgtc ttttttacca aattcaaggt aattttcaag atatttacca attggaattg
    25321 aaataaaatc catatttgcc ataatattaa atacccttac acctgatttt ccaagagttg
    25381 ctgctgttgt ctctgattct aaggttcccc caattgttat tacaccatgt tgccagtcaa
    25441 aactttcaaa aacaggtggc catgtatcag agtccctccc accaaaaata attccactga
    25501 cctcaacacc ttctttattt tcaccttcag ggtcaaaatt ttcaagagaa tttaacctta
    25561 tagtgtatct tgcatttgga tgtgaaggag gtatttcttc tccttcttca tcttttttcc
    25621 ctttccacca tttacctgaa aaatttattc cttcatcagg tatttctaat ccacatccat
    25681 tccagaaaac attcccattt gaaagtaaaa cattagaaaa aattacttct cctggtgtca
    25741 taagggattt ccatataaga gggtcatctt tttcattgac atcctgtata atcccaaaaa
    25801 ttcctctttc aacattagca gcataaacat taccatttct ttttttcagg taggcaatat
    25861 catctcctat aattgtttct ccttttacca tacaagtaga tgttttgcca cacatactcg
    25921 gaaaagcacc taaaaaatat gacttcctcc ccttcccttt tacacccatt ataaacatat
    25981 gttcagccag ccatttttct ttatatgcct ttcttattgc caaacgaaga gcaagttttt
    26041 tcaaaccaac tgtatttcct gcatattgag tattaacact ataaacagta tcttcaagaa
    26101 aatcaacata aattcttttt ttatcaaaat ttttacttgt tttatcttca gttaactctc
    26161 cagcagaatg aacatactta aaaaaatcaa tatcttcctt ttcggtaaaa acagaataag
    26221 caggtctata taaaatatct tcagaatgag caacataagc agagtcggtt aattgtattg
    26281 aatagataga aaatggggaa ttaacaggac aaagacagag aaaaagaaca tacatttctt
    26341 tccctaccat tatattttta agaagttgca taatttcttc atatcccttt tctctatcag
    26401 tataattaag taaagaacct aattccatcc cttttgttac cagcaacttt gtattacctt
    26461 tatctcttcc ctggtcataa tatccatcaa aatgaacagt atgcccatct ttttttaatt
    26521 ttatttcctc tccatactca attgctttat tccttatgta ttctatatcc tcttctgaat
    26581 cacttcttac aaaaacactg tccggattac atagagaaac aaaataagaa ataaagtcca
    26641 gtactttttt attttttaca ttacttaact tttcataatt tttttcattt agttttgctt
    26701 tcaagttgtt atccatagtc tctcctttaa ccaatcatta taccaaataa aaaaaacacc
    26761 cgtcaacttt tttatgccat tcttacatta gaagtataat aaaaattaag ccccctcacc
    26821 ttaatccctc gggcgtagtc caacgggcga gcctcgcccc acctatccct tacccacatc
    26881 ttctaaaatc ctcctgggta aaaggattcc ctttgctcct attttctttt ataaaattga
    26941 aaaatactca cctttttctt actttatcac ttttcaaata atacaacaaa gtttgtggta
    27001 tatttttcaa aatccactct gttccttcat cttccttcct ttgtaaagga aggttaggat
    27061 ggattttcct gtatttcctc ttataaaatc cccctaaccc cctttgaaaa gggggaactt
    27121 atgggtaagg gatagcccct ttttcaaagg gggaaatgac gcaccaactc tgtcttgcta
    27181 atctttgcta cctcagtcgg tttttcaaag tgggaacttg tgggtaaggg atagccacag
    27241 gagataggaa agagaatgaa gttatactta catgtatgat ttaaaattga tatttcatat
    27301 aatttttttc ctttacccag tttttaaaat atgatataaa attatagtat gaaaaagata
    27361 attgctgttt gtggaagtaa tgaaggtgat gaaaacttaa attcagatgt tcttgaaata
    27421 gcagaagaag ttggtttttt aattgcaaaa aatggtggta ttcttatttg tggtgggtta
    27481 ggtggtgtta tggaagcatc tgcaaaagga gcaaaaaaag gtggtggaat aactgttggt
    27541 atcatgccat ggaaaaaaga caaagcaaat aaatatattg atattccaat tgaaacaaat
    27601 cttggctttt ataggaactc tataattgtc aattcagcag atagtgtaat aggaatttgt
    27661 ggtagatggg ggactttaaa tgaaatttca atggcaattg ctcttggaaa accaacaatt
    27721 ttaatttcca caagtggtgg aatatgtgaa ttattttcag ataaagaagt tctcaaaaac
    27781 tttaaaacca aacccaaaat agcaaaatcc ccacaagagg cagttaacct tgctttctct
    27841 ctataactta accgaattta aaaaattcac caaaccttac ttttgttatt tttgaagata
    27901 tgacaatgca gacagaaatt attatcatcc ctaaaacacc aagcgcacat gcaacataag
    27961 gaccatctgt tacccttcct gcaagtgtat atataacttt tgcaacagga taaaattttt
    28021 ctcttattgc caaaattaaa ctactgctca cttccataaa tgaaaaagca aaaacaaaaa
    28081 tacaaccagc aaataatccc ggacttacaa gaggtaaagt tattttttta aatgtggtat
    28141 tatttgttgc tcccaatgaa tatgatgcct gttccatttc ttcccctata agttgaaatg
    28201 atgaaaatgt tgacctgaca ataaaaggaa gtcgtcttat actataactt attatgagta
    28261 aaaaataagg attatgtcgt gggtcaatta ttgtatttga aaaacaggca aaatatccaa
    28321 aagcaattat aataccagga ataactattg gaagcataac aagtaaatca agtaattgct
    28381 ttcctttaaa attttctctt gccaaaaaat atccaatggt aaatccaaga actgcgtcaa
    28441 ttaaagcagc ggaagaactc aaaaataaac tattaaaaat tcctgttgtt gttaaatggt
    28501 gtgtaaaaac agttttataa aattcaccag tatattctga tggaaaaatt gtaaaaaacc
    28561 attttttagc aaatgagttc aaaattattc ctacatgtgg agttaaacta aaaagaagaa
    28621 gtaatatagt caggaaataa aatactttcc cttttctatc taaatttttt ccactttcta
    28681 atcttttaat ccttccagat acataaggtg ttttttctat atattttttt gttaaaacaa
    28741 ataataatag agttataaat gtaattataa gaacaagagc atatccaaca ggatttgtat
    28801 ttatatcatt tatatgattg aaaacctgaa tagatataag atttgtataa tcaaacacaa
    28861 gaggagtccc gaggtctgtt aatgaccata taaatataat tgaagaagca gcaaaataac
    28921 cgggaagaaa taaaggaaat gtaatttttc taaatgtttg caagaaattc gctcccaaat
    28981 tatatgatgc ttcttcacat tcaatatcaa aattacttaa tgtagtggaa atattcagaa
    29041 acattattgg ataaagatga agtgtttgaa gaattacaat tcctgcaaaa cccgttccaa
    29101 tccatgagat tggatttttc aaaataccat acttaattaa aattaaattc aaagaaccaa
    29161 accttgaaag aatttgtctt atcccaagcg ctcctacaaa aggagttgca ataagtggaa
    29221 ggaaaaaggc aattcttaaa aaattctttg ctttaaaatt atatttataa aagaaaaacg
    29281 ctaaaggaat accaattata ctcgtcagaa aaacaactat tatacccaaa gaaaaacttt
    29341 ttataagtga agttcttaaa atataatttc caattgatgc cttcaaaatt tcaagagtaa
    29401 atttattatc cacaataaaa ctttgagaga taatataaga aagaggataa attaaaaaaa
    29461 gaattaaaaa tataaaatta aaaatataaa tagttttttt catttttcaa aaaccataaa
    29521 tttttttact gagaaggaaa ttttttctcc aatttgtaaa tcagtgaaat tttcaactaa
    29581 aacacatata ataaaattat ttcctttttt tgttttaaga gagacccgcg ttatttctcc
    29641 taaatactca aaatcagtta ttttaccttc aattagattt tcttttcctg aaaaagatat
    29701 aaattctggt ctaaaaccca aagtaatatt gtctcccctc ttgaaattag aaaaattaga
    29761 aacaacaaaa tccccttctt ctgtctcaac aaaaatttcc ttcgcttttt cttctttaac
    29821 tttgccatca ataaaattca cctctcctat aaaagaagca actgacctat ttatcggatt
    29881 taagtaaata tttagcggtg tatctatctg aacaattttc ccttcatcca taatagcaat
    29941 tcttgttgca agagacattg cttctttctg gtcatgtgta acataaatca ttgttgtaga
    30001 tgtttccttt tgtattcttt ttatttctct tcttaaactt tctcttaatt ttgtatcaag
    30061 attacttaat ggttcatcta aaagtaaaac ttttggttca attacaagcg ctcttgcaag
    30121 tgctactctt tgttgttgac cacctgatat ttgatatggg taaaggtctt ttacattttc
    30181 taatttagtt atttttatta tttcatccac tttcttatca ataaattctt tcccacgatt
    30241 tcttatttcc agtccatatg caatattatg ataaacagtc atatgtggcc atagagcata
    30301 attttggaaa accataccta ttttcctttt gcttggatgt aaattagtta tatcagtgtt
    30361 ttcaagtaaa attttccctt tatcagccac ttcaaatcca gtaattatcc tcagaagtgt
    30421 tgtcttacca caaccagatg aaccaagaat aaaaaaaagt tctccttttt caacagaaaa
    30481 atttatatct tttaagacct tattccctcc aaaacttttt tcaatatttt taacaactaa
    30541 aatttcattc ataattatta tatcctcttt tcttttataa tacccttata tttttttatt
    30601 gaaaaattca accactcatt tatatattga tttctaaata tagaattatt ccagttgaac
    30661 cataacaatt tttgttcctc ttcctcaaaa ggtatctgaa aaaaaattct tttaagagaa
    30721 ttattttctg aatcctttac aattttccat gcttttttaa ggtaaggatg acaatcaata
    30781 gcaaaacatc ctatcatatc atttaaaaca tcccatcttt ttgttgcaag aataaaatta
    30841 tattttattg tttgtcctga tttataagga tttatatatt tatttttcaa atcaggatga
    30901 ttataaacat ccgggagaat actaaatcta ttcaaagaat attttttagg accaaattct
    30961 ttaccttttt caagcatcca gagaagttgc ccttcataag aaagaagaaa atttataaac
    31021 ttttttgcta cttccatatt tggggctcct tttaaaatac caatagcatc aggatttata
    31081 actgtaagat tttctggaaa gaaaaaaccc aaattttctt ccccatttat ttcaatttga
    31141 gaaagagcat aagaatcaat acataaagaa acagcaactt ctccaattga agtatccttg
    31201 gcaatctggg cagcagaaga agaaaatgtt tttgtatttc cagcaatagc aagtattgtc
    31261 tcccatccct ctttccaacc atatgcctgt aaaattatct cgtacatcat atgcatactt
    31321 ccactatgtc ttgggtcagc ggcaccaaca agagaaaaat attctttcct gccaaggtct
    31381 ttccatgtct ttggaatagg taatgagaaa tactcaagaa cctttttatt atataaaatt
    31441 ccaaatccac ttaaaacaac cccataccag taaaaatcct tatcataatt tggaactcct
    31501 gcacacattt ggggtatttt tttaagtatt tgagaaggaa ctttatattt ttcaagatac
    31561 cctaattcct ttaaccttaa atatgggtcc attcctccac caaaaaatat gtcaatttca
    31621 ataccagtag ggtttttttt aaaaagtgat tcaacaaatt tcaggtcatc agaagttcca
    31681 ccctggtcaa tccattcaat ttttactttt tcaccataat tttttaaata ccatttttca
    31741 aatgcatctg aaacttctat ttgaactccc tcccaatgtg gagaaataat aacaagtttt
    31801 tcatctccaa tcaggtcaaa ggaaataatc ataaaaaata aaagaaaaat tctaatgtat
    31861 tttttgtatt ttgatttcat ttttaagccc taacacataa cttaagtcct tttatatttt
    31921 agtgacaaaa gcctgtctgc gtacggatac gcacaggcag gtactaaggc acaaaggtac
    31981 aaatcgcgta ttttagcgtc cgcttcgctt tgtggcggat ttaagtcaac atttccccct
    32041 caccttaatc ctctccccac aggggagagg gcggggtgag ggggcccttt tgtgctttta
    32101 aattaatgtg gttgaataag tatttaaatc ccaacattat aactgggttc ttatttacaa
    32161 agaggagaaa atactattca acataattac caatctcact tttaacataa tttttcagta
    32221 tcccaattcc ctcaatctca acctcaacaa tgtctccatc cctcaaaaat acaggttgtt
    32281 ctcttgcata tccaactcct gatggagtac ctgtcattat aactgttcct ggcagaagtg
    32341 taaattgctt tgataaataa ctgacaactt caaaaggtga aaatatcata tatgaactat
    32401 ttgagtcctg cataactttt ccattaactc ttgactgtat tcttaaatta tctggattga
    32461 cttctgtttc tatccatgga ccaatagggc aaaatgtatc aaatgattta cctcttgccc
    32521 attgtttatc cctcttaaat tgacagtccc tcgcacttac atcatttcca catgtatatc
    32581 caaaaatgta atttctaact tccttttcag atacattttt tgctcttttg ccaataatta
    32641 tgcataactc agcttcataa tcaacataat caggagcaac agaaggtaaa aatattgggg
    32701 atagatgacc tgttattgaa gttgttgctt ttaaaaatat gactggttct tctggtaaat
    32761 ccattttgct ttcttgggca tgttccttat aattaagtcc aagtgcaata atatttggcg
    32821 gaaaaacagg gggcaggaat ttaatttctt caatatttac tctttcacct gtttcctcaa
    32881 aatcatcaat aaatggttgc catttaataa cttcaataaa atcacctttg taaattccat
    32941 aattttcttt attatttttc aagaatttta caattttcat tttccccttc ttttccaaac
    33001 aattatcaaa attatctgag aatttatatg tttttaccaa ctttcttttt tctttctatc
    33061 ccttcctttg gtaaaggaag gttgagatgg atttctctat ataaactcac actttctctc
    33121 aaaaaaatcc ccctattcct cccacctacc accttgtaaa atcttatcac ttttttgtat
    33181 aaattcaaat ttttatatta ttttttatta tttcatcagg tatataatat cagaaaactt
    33241 gactataaga ggataaaaaa ataaaatata taaggaaaaa gcgcctgtag ctcaacagga
    33301 tagagcatct gccttctaag caggtggttg cgcgttcgag tcgcgccagg cgcaattttt
    33361 agaccatccc gcctactggt aaaaagattg ataaatactc aattgtctat attaacttac
    33421 aatatattaa aatatatcaa ttaaaaaact attttatcca ttacacctta ttctttctca
    33481 ctttcctctg cctttaattt ctttatactt tctgcttcaa tctcattata aaacctatat
    33541 tttcccacat acttaacttt ctgaaatgtt ccaataactt ccaccttatc attattctta
    33601 atatctttgt ttttaatttc ctgatgttcc cagataaaaa cctttattcc attatagtca
    33661 tcatctatca aattaaaagt agtataagga ttatcttttt tagataccgt taacttaatt
    33721 ttctccactt tcccttcaac aataacttcc tgtttatcat acttatcagg attgtctaaa
    33781 atctctttta ttgtcgtctt ttgtggagaa gcaaaaacaa gagaagaaat aatgaacgaa
    33841 actccaataa atccaacaat gaaaccagaa aaactctttt cccctttcat tttaccacct
    33901 ccatacaatt cctacagacc tggtctgtag gaaaatccct tcccaatttc ttttagacat
    33961 ttataatatc tgattttata ctatcccata a
//
LOCUS       DAOOVW010000015        33288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_879_length_33288_cov_7.302079, whole genome shotgun sequence.
ACCESSION   DAOOVW010000015 DAOOVW010000000
VERSION     DAOOVW010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 33288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33288
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_879_length_33288_cov_7.302079"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..1304
                     /locus_tag="PLW95_03345"
     CDS             129..1304
                     /locus_tag="PLW95_03345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOV21699.1"
                     /translation="MERIYKRKILILFFVAFLLRFAFILTLKNHFYFDDEYEYYKMVK
                     NFLSGNGLIVGENLKSFRPPLYPLLLSLLYSLNFNLFFIRVIQCLISAATVIFIYFLG
                     KKVFNEKTGLISGIISAFYPFFIFYNGFLLTETLCVFLVVISIYYYSLCLISKEEKLN
                     EIKAGIFFGLSGLCRPTLESFFPFAMVYLLFLKEIPIKKRLQKIILISLFFVFTISPW
                     IIRNYVIFKKFIPGTTMGGWVFWEGNNPKSDGGPCSYFPENILQMEELARDKFLYRKT
                     IEVIKQNPSRFVYLLKNKFVRFWNITLNASDFQKPLYKILSIISFGFLLPFFLIGFFI
                     TFKNKNALIMHLLIIFFTIFHMVFLASIRYRVAIEPFYIIFASYQLTKLTSIWKSRS"
     gene            1307..1951
                     /locus_tag="PLW95_03350"
     CDS             1307..1951
                     /locus_tag="PLW95_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013887015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOV21700.1"
                     /translation="MDKRIFYKDGVFHIFTRSIAEYKIFKDNNDYLRFIEMLKFYQYK
                     RNCSFSYYLKTKEIPQKNLDKIGEKIIRIISYCLMPTHIHLILSSEVEDGITKFMKNL
                     LDSYTRYFNTKTKRKGPLWESRFKSVLLETDEQLFHLTRYIHLNPVTAYLINKPEEWQ
                     YSAYSEYLDSIKQEKKICEYDNLLEINPEKYREFVENRISYKRELAKIKKIVFD"
     gene            2008..2685
                     /locus_tag="PLW95_03355"
     CDS             2008..2685
                     /locus_tag="PLW95_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOV21701.1"
                     /translation="MKLSVIIPVYNERDTINKIIEKVRKVPIEKEIIIVDDGSTDGTQ
                     NILKNFNNKDIKVILKEKNEGKGMSIREGLKYVSGDIVVIQDADLEYEPMDFLKMIEV
                     MKRNNAEVVYGSRILGKGKKSSFFFYLGGRFLSCIVNILYNAKITDEPTCYKMFRTDV
                     IKKINLKCTGFEFCPEVTAKVRKMGYKIFEVPIHYIPRSIKEGKKIRLKDGIIATWTL
                     IKYKFID"
     gene            2728..3504
                     /locus_tag="PLW95_03360"
     CDS             2728..3504
                     /locus_tag="PLW95_03360"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOV21702.1"
                     /translation="MVKCPICKSRSRLIWKEKKHKVFRCKNCKIAFLYPIPESSEKIY
                     NRKYFENWYLRYYDERKRYFEKLFNNKLKNYLPEQGKILDVGCGVGIFLDIVKEKNYD
                     VYGQDISPFAVEYCRKKGFNIFDKPLPEISLPENFFDIITIFDVIAHLGNPVEYIKIC
                     KRILKPGGILIIKTPYQPNYLFFLANILSFTGKSKGLLHIPAQIFHFSYKTFEYMSKN
                     FNFSKIFMFNIREFFPDIISISLLKKFVLNEKSIIFIAKK"
     gene            3501..4970
                     /locus_tag="PLW95_03365"
     CDS             3501..4970
                     /locus_tag="PLW95_03365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOV21703.1"
                     /translation="MNNNRIRLFFLIIIICIGIFLRIFQLGKLCFWCDEFLAIFCGWQ
                     PLKDMINYITYKDAHPPLFYALVHFLLKYGNSEFYLRLLPSFFGIITIPLAYFVGKEF
                     KDEKTGIIFSIFISLNPALILWSRILKSYSLFTFFLLLSLFFFLKILKTDKKQYYIFL
                     FFSDIILLYLHNFAFIWILIEVFTLLFNKIFNKKFLFYYIFIFIFYLPWFLKIPYQLK
                     FTLGVIRPIPFVLRYPYLFFYFFFGETLHPFNLKIVIPFLIVIIFIVVNSFIDLKKLE
                     KVKKYLLFLGILIPLFLVPFPSTVPQNLLPFSIFVFLLLSIGYKNTNFTKLLFFCFFI
                     LSSFSTYFYFTGNTKNFHDVSKLIPYREINEIFSEKLDENSIIFLNGTDQDSMYKKFS
                     PFHWYYKGNTKVIEVAKRPGSIAEIKKICKSYNKIGLFLNYNEDQEWNNELKNFFFEN
                     FFPVYQAKFLHNEKLLSRLKGKKEYYWFVEIYIFEKHKK"
     gene            4986..6026
                     /locus_tag="PLW95_03370"
     CDS             4986..6026
                     /locus_tag="PLW95_03370"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015909115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOV21704.1"
                     /translation="MKRILITGGVGFIGINLAKNLLCEKDNFVVLYDNLSRKGVENNL
                     EWILSFNFPNLKFIKGDIRDYKKLKEIVKNFDEVYHLAAQVAVTSSVENPIDDFEINA
                     LGTLYLLEVIRKNSPDAAVIFSSTNKVYGELLNLKLKEGEKRYYLIKGKEGIDENQNL
                     DFHSPYGCSKGTADQYIRDYYRIYGLKTVVFRQSCICGQQQYGNEDQGWVAHFIIKAI
                     EGEKISIYGDGKQVRDILEVDDLISAYKIAVKNIKKTKGEIYNIGGGKENTFSLIELI
                     EYLEKVLRRKITYDFYDWRPGDQKIFISNNKKFMEETGWRIKINKFEGVNRLIKWVEK
                     NHELIKKVREKK"
     gene            6023..7360
                     /locus_tag="PLW95_03375"
     CDS             6023..7360
                     /locus_tag="PLW95_03375"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012963015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOV21705.1"
                     /translation="MRNIGVIGAGHVGLVTGACFAKMGNIVICCDNDIEKIKKLKNFK
                     IPFYEPHLEEIVKETFKNKKLTFTTSIKSLVEKSEIIFIAVGTPSIESGRADLSSVEN
                     VTIEIANALSSIKKQKGQNYKIIVEKSTVPVLTGEWVKKTLNLLSPTGIEFDIAANPE
                     FLREGSAVYDFFHPDRIVIGIENERPKKIFEEIYKQIKCLKLFTDIKSAELIKHASNS
                     FLALKISYINAISQICERVGADVEMVAEGMGLDKRIGKQFLKAGVGYGGSCFPKDVSA
                     FISLSEEIGYSFTLLKEVQKINQQQRQLVVKKARNLLWNLKGKNIGILGLAFKPNTDD
                     VRESSAIEIIQLLKKEGANIKCYDPQASDNMKKIIPDIIYCKNPYDVAKESNLLIFLT
                     EWDEFKKLNFKKFKEIMKTPYIIDGRNFLDYKNILKCGFIYTGIGRKINENSY"
     gene            7344..8285
                     /locus_tag="PLW95_03380"
     CDS             7344..8285
                     /locus_tag="PLW95_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013433594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOV21706.1"
                     /translation="MKILISGGSGFIGSHVCDYFLKEGGNVWCIDNFLTSNIENIENC
                     LNNKNFVFIEDDICKINVDKLKNNFDYVLHFASPASPVDYNKYPVETLRVNSIGTENM
                     IKISLANNSIFLYSSTSEVYGDPEIPVQNEEYWGKVNPIGERSMYDEGKRYGEALIMA
                     YHRKYKLNSRIVRIFNTYGPRMRLNDGRVIPNFIVSALTGKPITIYGDGNQTRSFCYI
                     SDLIKGIIRILKCNYNLPINIGSCEEFTIKQLAKKVKEITGSKVEIIYKPPLPDDPKR
                     RKPDITKAKKILKWEPVVTLENGLKETIPYFEKKLKK"
     gene            8282..9400
                     /locus_tag="PLW95_03385"
     CDS             8282..9400
                     /locus_tag="PLW95_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009761141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOV21707.1"
                     /translation="MKKILVTGGCGFIGSHLVDKLIELGYEVIIFDNLTKQVHPSGIP
                     EYLNLKSTFIRGDVRNRNKLKDVVKKIDIIYHFASAVGVGQSQYEISKYVDVNIRGTA
                     NLLDILVNEKHNVRKLIIASSMSIYGEGMYKCKKCGKVKPEIRNFDNKKIDDWEPKCP
                     NCKGDITAIPTDEETPTKSNSIYAITKKEQEEMSLLIGKIYGIPVVSLRFFNVYGPRQ
                     TLSNPYTGVCAIFLSRIKNNKPPIIFEDGMQTRDFIWVDDIINACILSIQKDQANYEI
                     FNVGSGKPTYLKEIAEILIKLLNKNLKPEITYKFRKGDVRHCYADISKIKEKLGFKIE
                     TELEEGMKKLIDWSKKEKKVKDKFDYAEKQLKNRHLIE"
     gene            9393..10103
                     /locus_tag="PLW95_03390"
     CDS             9393..10103
                     /locus_tag="PLW95_03390"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOV21708.1"
                     /translation="MNRIELTIVIPARNEESNLERTVEDIVQYIDTNTTEIIIVNDHS
                     TDKTEQIGQQLSLKYSFIKVINNKNEPGFSTTLLTGFKQAKGEYVLPVMADMCDDPGS
                     IQKMLEKAKDSYDIVCGSRYTKGGKKIGGPKLQNFFSFFVCKSLRYLINLPTNDVSNA
                     FKLYKKNIFNIIKPKEKGFAISMEITLKSYFYGLKITEIPTTWYGRKKGKSKFKLIKT
                     FPYVKLYFWAILKKWKFL"
     gene            10088..10789
                     /locus_tag="PLW95_03395"
     CDS             10088..10789
                     /locus_tag="PLW95_03395"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOV21709.1"
                     /translation="MEISIIIPSYNEEKRIGKSLEKIWKYFKNKGFPFEIIVVDDGST
                     DKTVEIVEKFKEGKKEIRILKHDKNKGKGAAVRTGVINSKGNLILFTDADLSTPIEEF
                     EKLKKAIDDGYDIAIGSRGLPESKIIIPQPWYRRYLGKIFPLLVRILVTNKFKDTQCG
                     FKLFKRAVAKKLFDELKTNGFAFDVEILYNSIKMGYKIKEVGVIWSNSFFSSVAIFKD
                     PLKMFISLLKIRSFK"
     gene            10789..12234
                     /locus_tag="PLW95_03400"
     CDS             10789..12234
                     /locus_tag="PLW95_03400"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOV21710.1"
                     /translation="MKVLFTYIPQPFSKKGYLLVSQNRFTKWRGTKELIYPLIPASGL
                     TLLDLKGYEVYYLDCIFEEIDEKNFFEYIENLKPDIIFTETKTPIIKYHWIISEKIKR
                     KFPQIITCIIGDHITVLPEETMKNSKFDFVLTGGDFDFYMLQLVQFLSGKEKIPSGLW
                     YREKNGKIKNTGKFILIDNLDSLPFINREIVPWKNYHEAWRISENFMYLSGSRGCPYR
                     CTFCSWPQMLFDNKIRYRSPENIVNEIEFLVKKYKTEEFFFDDDTFTFNKKWCIEICE
                     EIIKRNLKIMWSCNGRVDNVDEFLLKKMKDAGCRLIKYGVESYSQYTLNKIKKGYTIQ
                     QVKKAFSLTKKRKILIHATAMIGFPWETRKDILNTIGFIKSLKPDTCQFSIPITYPGT
                     ELFKEAEEKNWLKFNYNWEKYDMSLPTLKNLYLSDDKLVKLCKLAWGKIYLDPLFILR
                     KVLRIRTISEVKWLIRGFISFLFGHVKPIQK"
     gene            12231..13190
                     /locus_tag="PLW95_03405"
     CDS             12231..13190
                     /locus_tag="PLW95_03405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOV21711.1"
                     /translation="MKTYFVIIIPCKGYNEYLNKTIKGCLNQTFKDFKIYLLPDNNFE
                     NNIFKNDKIVIKSTGNYFPGKKRNIGIEISEAEVYAFIDSDAIPERVWLEKAYNYLKD
                     EKIKIIGGPNLTPAESNLREKISGILLSNFLVTGKFSIRYKKNLKLETVLELPLCNLF
                     IKNEVFKSVGKFKENTLTAEDADFCFRAKKLGIDTKYSSEIVVYHKRRPIFLPYLNQI
                     FQYAFDKGYRIKKNFVPKYLYYFLPSFFLIFLLIGLYSLIFFKNFLFFYCFFISLYFF
                     LVFISCLINWKYLFYIFSGIILTHLVYGSGFIIGLFSKRENEN"
     gene            13180..14901
                     /locus_tag="PLW95_03410"
     CDS             13180..14901
                     /locus_tag="PLW95_03410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOV21712.1"
                     /translation="MKIKEFVVLFIISSISYFCGLNWGLPSKSLNKLYFNDIETIEKH
                     IEQIKKYPIEKIYKGMGPYLAKYPEVEKEKLPRFYYNPIRSYHPDEYYVIKSFSSMKP
                     EKFDFNPHAYSIGGAYLYLVGLFLFLFSKLNFVVLSKDICFYFLHPEEIGKFYIIGRA
                     ITAIYGIGIILLSYLLCKKFFKDGLKSFLICLLILFSPLIILNSHYMYVDIPGLFWIT
                     ACLFITSNSIEKFSFKNVFLAGIFAGLACGTKITFVVSIVIPLLGIFIIFKNPKDIVK
                     NFLLVVICFIFSFFITNPYFFLTFPEPLIELEQHTGLSFNGKFYFLSLKYGLGFPLLI
                     FALTGTLLSIFFIKERSDLEKKLLFLLFSWTVFFFIFISIFSKNFARYILPLVPSFII
                     LGGYGWFLTLTKTKKILKFLISLWIIFVILFTFFYGMSYEMLFIQKNIRTEAGEWIKE
                     NIPAGSSIGVTEVPWQFQMPPFDYFKYNLIVTGYDFNKLKKEKPEYFIISSFQAPIPP
                     FPLKLQIEKNSFWNEFINSSIYKEEKKFQKFPSFLSFKFQFNELPEDLIYLNPTIVIF
                     KYKNETF"
     gene            14882..15886
                     /gene="queA"
                     /locus_tag="PLW95_03415"
     CDS             14882..15886
                     /gene="queA"
                     /locus_tag="PLW95_03415"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOV21713.1"
                     /translation="MKMRPFKFNLPKELISQYPLKEREKAKLMVVNRKNGKIIHDIFE
                     NIDKYMSEEDVLVLNNTKVIPARLLCRKETGGKIEIFLLKKIEKNKWNVLVGGKGKIG
                     SKFYKEDIAGKIIEKKADGSFIVEFNIEDENEIKKYGEVPLPPYIKRKAEEIDKIYYQ
                     TVYAEKDGSIAAPTAGLHFTEQLLEKIQKKGIKVVYITLHMGWHSIKVFKEDNTEKNV
                     STEFFEISQQTAEILNKSKKNKKNIIAIGTGTVRCLESSVENGIIIRKKGFTELFIKP
                     GYKFKVVDKLITNFHLPDSTHLYLVSAFTGLDLIEKAYNIAVEKKYRFYSYGDSMFII
                     "
     gene            16282..17391
                     /gene="tgt"
                     /locus_tag="PLW95_03420"
     CDS             16282..17391
                     /gene="tgt"
                     /locus_tag="PLW95_03420"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOV21714.1"
                     /translation="MFEFKIINKDKKTRARVGEFLTEYGKVETPNFMPVATLGSVKTL
                     TSEDLINMGVDILISNAYHLYLKPGIDIIKKSGCIHKFINWNKSIITDSGGFQIFSLE
                     NVKITEDGVIFKSKLDGSTHFITPEKSIEIQNKIGADIIMSFDYCPKNWNDYGEVEKS
                     VNYTIKWAERCKKYHKNKEQTLFGIIQGGVHNDLREKCINKLEILNFDGYGIGGLCLG
                     EPEEKTFEIADFISKKVNDNKLKYFMGIGMPLQILNLVEMGYDLFDCGMITHIARTGS
                     TITSRGKINIKGGKYKDDFIPLDPECDCFVCKNYTRAYIRHLINSKEITGLRLNSYHN
                     VYFIINLLKNIRKSIKEGKFSDFKEKFEKKYKEKN"
     gene            17471..17788
                     /gene="yajC"
                     /locus_tag="PLW95_03425"
     CDS             17471..17788
                     /gene="yajC"
                     /locus_tag="PLW95_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007799984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOV21715.1"
                     /translation="MKFLMLAQAQQQSTPSLLGALLPLILIFFVFYFLLILPQQKKQK
                     LHKKMLDDLKEGDKIVTVGGLIGVVSKIKDNIVTIDCGNGVKVDFLRNAISQVIKPQN
                     DNK"
     gene            17775..18929
                     /gene="larC"
                     /locus_tag="PLW95_03430"
     CDS             17775..18929
                     /gene="larC"
                     /locus_tag="PLW95_03430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HOV21716.1"
                     /translation="MIISKTGYFEVFNGCAGDMIVGCLIDAGVNPSILKNELQKIPIS
                     NYTIKIKKVKRKTNSSHFISATQFIVILKEKENQRKYTEIVNLIEKSSLSDDIKRKTL
                     KIFNILAISESKVHKEKIDNVHFHQIGQTDAIIEIASAVIGLKLLNIESVYSSSIGIS
                     MPSPATLEILKGLPVLIKHIPYEISTPTGVSILKGLCKFHEIDEKISIEGYGYGTGTS
                     ETLPSDIMKFIYGKEIDKKKEEIYIIETNIDDMNPVIFEYLYEKLFQAGATDVSVFTG
                     IGKKNRPVFNLQIMVNEENFEKVKEIIFSETTTIGFRYQKEDRIILQRKIKEIKTKWG
                     SVKIKISFYGGKIYNISPEYDDCKKISKKFKIPLKNVITEVNNLSKNISM"
     gene            19115..19441
                     /locus_tag="PLW95_03435"
     CDS             19115..19441
                     /locus_tag="PLW95_03435"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HOV21717.1"
                     /translation="MKYKKDFTIRKMFVGTIKIELLIPGCKSLKDKRNIINSIKNRIK
                     TKYNVSIVEVDFYEKWQRAAIGVSFVNGEAKSTEILCQKILDLFFDNNDFIVLNNKNS
                     IISIED"
     gene            19448..22093
                     /locus_tag="PLW95_03440"
     CDS             19448..22093
                     /locus_tag="PLW95_03440"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOV21718.1"
                     /translation="MEKQYNPKAIEEKIYNIWEYNNFFHCEADKKKKKYVIVIPPPNI
                     TGFLHMGHALNNTIQDILIRWKRMEGYNALWVPGTDHAGIATQNVVERKLLKEGKKRQ
                     DIGREKFIEEVWKWKEEYGLRIIDQLKKLGASCDWNRLRFTMDENLSKAVKYAFYQLY
                     KKELIYRGRKIINWCPRCTTALSDEEVEYKEIPSFLYYIKYPLIEGDFIEVATTRPET
                     MLGDTAVAVNPLDKRYKNLIGKKVKLPFVDRVIPIIADEKVEVKFGTGAVKVTPAHDP
                     TDFEIGQRNKLPFVIVIDEEGKMNEEAKQFNGIDRFLAREKIIEQLKNNGLVSKIEPY
                     IHRVGHCYRCLTPIEPYISTQWFVNMKPLSKPAIEVALEKKLKFYPEKWEKIYLNWLY
                     NIKDWCISRQIWWGHRIPIWYCENKDCPPIVSIEDNVKKCPNCGSENIKQDEDVLDTW
                     FSSWLWPFSVFNWPEKTDDIDVFYPTDTLVTAQEILFFWVARMVMAGMEFMKEIPFEN
                     VFIHGTVRDETGKKMSKSLGNAIDPVDIIENYGADSLRFSLISMTSFGQDVYLSPTFY
                     LKGRNFMNKIWNAGRYIITINEEMGTNDIKIYENIDFNNLKFPEKWLILNLNETVKNV
                     TSLLDKFHFNESINYLYDFFWHTFCDWYIEISKIYKKENDYFKNVVIPILNKSLIISL
                     KLLHPYIPFITEQIWLSLKKYISDLESDYIIISKWPVVENYRDDDTIEKMNRIIEIIT
                     NIRTIKTKIKVPVTKIIDCYIEGEIDDIEKEIVESLGKVKIKEKQDIMKNKNYLLKNL
                     SSGGLFISIEGIIDIPEEIKKLEGKKKEFEILLEKTEKTLSNKQFLEKAKKEVIEKTI
                     NNKKDLEEKIENIKKDIEFFKGQIE"
     gene            22123..22434
                     /gene="rplU"
                     /locus_tag="PLW95_03445"
     CDS             22123..22434
                     /gene="rplU"
                     /locus_tag="PLW95_03445"
                     /inference="COORDINATES: protein motif:HMM:NF013025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOV21719.1"
                     /translation="MYGFIDIGGKQYKVEKGKKFDVFNLNKKVEDIVEFNPVCFFDDK
                     DLITEEDKLKNISIICKVLEEKKGEKIYVFKKKAKTGYKKGYGHRDHITVLKVEDIKI
                     K"
     gene            22431..23549
                     /gene="hflX"
                     /locus_tag="PLW95_03450"
     CDS             22431..23549
                     /gene="hflX"
                     /locus_tag="PLW95_03450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOV21720.1"
                     /translation="MKEHIKVIRDDNLSGERVLIIAFKKKDPIETDTSVRELEFLSKT
                     LRLNIVKTLLLKENLIHPSTYIGKGKLFEIKKIVDEYKITLVVFEKDLSPVQQRNIEE
                     TLNINVIDRTLLILYIFAEHAKSKEGKIQVELAQLTYLLPRLTGHGIELSRIRGGLGI
                     KGPGEMKIEVYRRRIKDRIAKLKQEIKEIEKHRNVLRSSKKREKFHAVSLIGYTNVGK
                     STLLNKLSSSNLYVANKLFSTLDPATRLVYIGDDKYVLMSDTVGLISNIPHHLIEAFK
                     STLEEIKYADILLCIYDVSTNIEKQKNTIYDVLKILNVEEKLRIDVFNKIDLLEKEEL
                     NIMKIQNPEKIFISSLTKEGFDQLREKIMEVFYGIGVK"
     gene            23530..24531
                     /locus_tag="PLW95_03455"
     CDS             23530..24531
                     /locus_tag="PLW95_03455"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOV21721.1"
                     /translation="MVLGLNKDDILKIKKISNIIEKNKNFLITTHQNPDGDAIGSELA
                     MFLFLKKQNKNVKIINCDKVPSIYKFLPSVKEINIQNKKNLKNNFDVIIIIDCSSPER
                     TIDISDYKKNSLIVNIDHHITNFQFGNLNWINPEFSATGEMIYFIIEKLKKLDRSIAA
                     CLYTAIITDTGRFTYRMSKFTMDIVKNLIEYKISPVEIATKIYLEKPYKSIKLLSLAL
                     NNLKFDFKNKVCWMKITRQLYKKTNTDEEFTEGFIDFLSEIKESKIVFLMKEKEDGIK
                     VSLRSKEKFDVEKLARKFGGGGHKKASGCFFKNKKIEEVEKTILNEIRKNGRNYKYK"
     gene            24506..25216
                     /gene="truB"
                     /locus_tag="PLW95_03460"
     CDS             24506..25216
                     /gene="truB"
                     /locus_tag="PLW95_03460"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOV21722.1"
                     /translation="MDGIININKQKGMTSYDVIRFIKRQFNFKEKIGHAGTLDPIAEG
                     VLIILIGNATKLSCKFMKFEKEYICTMELGKKTDTDDIQGKIIEEKEVKVTTKEIIET
                     IKNFEGEYIQTPPVVSAIKHQGKPLYKFYREGIIIKPIPKPVIIRDIDIEEIKLPFIK
                     IKVICTKGTYVRSLCRDIGDKLGCGATQVELKRIRIGPFSIEKTFTLQQLKKIGIENA
                     IITIQKLQQILKNEKEKF"
     gene            25197..26573
                     /gene="glmM"
                     /locus_tag="PLW95_03465"
     CDS             25197..26573
                     /gene="glmM"
                     /locus_tag="PLW95_03465"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR03990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOV21723.1"
                     /translation="MKKKNFKISVAGIRGIYPDEITPEIAFKFAFAFGFFGKGKTFLV
                     GKDTRISGDVLISSVISGLLSSGKNVLNIEITPTPMIGFIIEKIENKYGIMVTASHNP
                     EEYNGLKFFNKKGNFINNDEWKKFLEIIKSVKKLKLSSIPGEIKKIEKNYIKIYFDNI
                     YKLVDVKKIREKKFKVIVDACQGVSSIYTEEFLKNLGCDVLVFNKFPYGKFSHNPEPL
                     IENLQQLKEKVIKEKGDIGFIQDPDCDRFSFVCENADIPGEETTLAICLDSVLEKEKT
                     PVVVNLSTTSLIDDICKKHKVKIYRTKIGEINVVEKMRKIKSRAGGEGNGGVIWGKFH
                     YGRDSYIGISLLLEKMAEENKKISEIVNNYPKYYMIKEKYKIKNIKFILKKIKKIYKN
                     EKLDLTDGIKIIRENGWIHIRQSGTEPVVRVIVEATTKITADEYLKEIYSILSARPGM
                     PDQVWRST"
     gene            26600..27199
                     /locus_tag="PLW95_03470"
     CDS             26600..27199
                     /locus_tag="PLW95_03470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOV21724.1"
                     /translation="MEILNEIIKTSIMLFIVIDAIGGIPIFISLVPDISEDEVKKVVN
                     LAIIVSFFILLFFGVIGRWILYLFNINLEEFKIAGGFLLLIIALDEIFNILPERKYPK
                     EELGIVPIGCPLLAGPGAITVILVILYRFNFPQNYIIMSASVVIVTLVNWIILSRLIN
                     IKRIFGRKGILLLTKLMGIILAGISINFLVSGIKNLFTG"
     gene            complement(27196..27843)
                     /locus_tag="PLW95_03475"
     CDS             complement(27196..27843)
                     /locus_tag="PLW95_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOV21725.1"
                     /translation="MNKEEKIYAEESWRIFRIMAEFIEGFEELGNVKDAVTVWGAARV
                     KEGDIWYKKAVELGKLLSKNGYTVITGGGPGIMEAANKGATLAGGNSIGLNIELPHEQ
                     KPNPYIKTLLSFKYFFTRKVMFVKYTKGFVIFPGGFGTLDEFTEAITLIQTGRIHKFP
                     IILFDSSYWKGLIDWMKNTQLKRGYISPEDLLIFSIVNEPEEAIKKIKDFYKKID"
     gene            complement(27843..29141)
                     /locus_tag="PLW95_03480"
     CDS             complement(27843..29141)
                     /locus_tag="PLW95_03480"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HOV21726.1"
                     /translation="MRKIILFLPCFFLISSFCFSENKAYTLNINDAIGPITYYQITRV
                     INSAEKNNAEFVLLIIDTPGGLLSSTRKIVQEILKSKVPIIGFVYPSGAQCASAGTFI
                     ALSCDILVMAPATNIGAAHPVTLTGGDEQNKTMEEKVVNDTVSFIKSIAKYKGRNEKW
                     AEKAVRESISSTETEALKNNVIDFIAEDIDQLLKKLDGKKIKKNEKEIILHTKDIVFE
                     SLKQSFKDDFLKTISDPNIAYLLLIIGMWGIILEFSHPGFGLPGIAGTICLILGFFAL
                     HTMPINIAGLLLLILGFILFGIEAITPTFGLFFISGIISFLIGSFMLIRPGSEIKIAT
                     SLIFTVAFASGFFIWAILLFALKTRRRKVTTGTEGLIGEKGRVKTDLNPEGLIFVHGE
                     YWKGIPKDKNEIIKKNEEIKVIGKDGPVLIVEKINKEGDR"
     gene            complement(29138..30214)
                     /locus_tag="PLW95_03485"
     CDS             complement(29138..30214)
                     /locus_tag="PLW95_03485"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HOV21727.1"
                     /translation="MEKETKIERVRRYIKENGLSGILVSGVSNFSWLTGGKKNFVGLN
                     TEKGVCSILITPDKSFLLTTNIEYPRIFEEEISDWEFIVKNWYKENELFEEVKKICKG
                     KIGSDIPTNFTVLVKLEKLHFPLVDEEIDRYKEVGKLASESITEISKVIKPGMKETEI
                     AGIVSQKLWEKDIIPVVILVGADERIEKYRHPIPTEKKLDKRVLIAICGKRYGLIVSL
                     TRIVNFGKLEESILRKHKSVCFVDTVFIDETRPDVKMSDIFKKAKSSYKETGFENEWQ
                     LHHQGGPTGYSTRYFRVTEETDEKVIEKSSFAWNPSITGTKSEDTIIIEKDKNTIITE
                     DKNWPMIEVEYKGKIYKRPAILEK"
     gene            30692..31642
                     /locus_tag="PLW95_03490"
     CDS             30692..31642
                     /locus_tag="PLW95_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21728.1"
                     /translation="MSKTEKVKEYAKKCGADLVGISSPERFEGAPLQMDPRQIMPGVK
                     SIIVLGFRVFRGTFRGIEEGTFFSNYSSMGYGGISNIQMPMVLWNFCKIIEDKGYEAI
                     PLGHLSPWRAIDENGEIKKGYSRPVAPGKPTPDVMIHHRIAAFCAGLGEIGYSKVFLT
                     PEFGPRQRLGEVLTDMPLDPDPLYEGPAICDRCMECVKGCPAGAISRIKTVKVEVAGK
                     MLEWGELDVEKCSIGLRGGRKVKEGEKGDYLQGRSDVKPGMHSPFYHKPPNVFIHGEA
                     ICGGRGCIRACMIHLEKQGKLKNKFKDPFRKETPWRVNWD"
     gene            31697..32473
                     /locus_tag="PLW95_03495"
     CDS             31697..32473
                     /locus_tag="PLW95_03495"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOV21729.1"
                     /translation="MANVQEIKIKSSVDGTTQPNIIITSSSYNIENFVPLLVALHTWQ
                     GDYKQLVKPIEKQIEKRNWVALLPNFRGPNCLENPDCKKACGSEYAQRDIVDAINYVF
                     SCYKIDKQRVYLIGGSGGGYMSLMMVAKYPNMWSAVSTWASISDLKKWWEENKNYSIH
                     IEACLGGPPGKSSEIDKEYYERSPINFITSAKNVWLDIHHGINDMVVPYHHSEDVYKK
                     LIDAGSKKARFTLFKGGHTINYFQACQWVSKKSNEIVVTT"
     gene            complement(32783..33031)
                     /locus_tag="PLW95_03500"
     CDS             complement(32783..33031)
                     /locus_tag="PLW95_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21730.1"
                     /translation="MWICFTILVVENFIDGCFLFLQEKIDITKEGIIIDTTGLPNQIN
                     MDINEWGYIDKIEEEIKFLFVVAKGKGLPLFFSIYPGI"
BASE COUNT        12964 a         3894 c         5153 g        11277 t
ORIGIN      
        1 attgtacaga aagcagggta taaaggggct gttgttaata gaaaaaaaaa tatgaaaata
       61 acaaaattta cattaccaag aattggtatt tattctcata ataatgtttt tatttacaag
      121 gtcaaaatat ggagagaata tataaaagaa aaattttaat tttatttttt gttgcttttc
      181 ttttaaggtt tgcctttata cttactttaa aaaatcattt ttattttgat gatgagtatg
      241 aatattataa aatggtcaaa aatttccttt ctggaaatgg tcttattgtt ggagaaaact
      301 taaaaagttt tagacctcct ctttatcctc tattgttatc attattatat tccctgaact
      361 tcaatctttt ttttataaga gttattcaat gtctaatatc tgctgctact gttattttta
      421 tttatttttt aggaaaaaaa gtatttaatg aaaaaactgg acttatttca ggtattattt
      481 ctgcttttta tccatttttt attttttaca atggtttttt attaacagag accctttgtg
      541 tttttcttgt agttatttca atatactatt attcactttg cttaatttca aaagaagaaa
      601 aattaaatga aataaaagca ggtatttttt tcggtctttc aggactttgt agaccaacat
      661 tagaatcttt tttccctttt gcaatggtat atttactttt tttaaaggaa attcctatta
      721 aaaagagatt gcagaaaatt attttaattt ccctattttt tgtttttact atttctccat
      781 ggataataag aaactatgtt atttttaaaa aatttatacc aggaacaaca atgggtggat
      841 gggttttctg ggaaggcaat aatccaaaaa gtgatggtgg tccatgtagt tattttccgg
      901 aaaatatttt acaaatggaa gaattagcaa gagataaatt tctttataga aaaacaattg
      961 aagttataaa acaaaatcca tcaagatttg tatacctgtt aaaaaataaa tttgttagat
     1021 tctggaatat tacgctaaat gcttctgatt ttcaaaaacc gctttacaaa attttatcta
     1081 ttattagttt tggattttta cttccatttt ttcttattgg gttttttatt acatttaaaa
     1141 ataaaaatgc tcttattatg caccttttaa ttatattttt tactatattt catatggttt
     1201 ttcttgcctc aataagatat agagttgcaa tagaaccatt ttatataatt tttgcttcct
     1261 atcaacttac gaagttaaca agtatatgga aatcaaggag ttagaaatgg ataaaagaat
     1321 attttataaa gatggagtat ttcatatttt tacaagaagc atagcggaat ataaaatttt
     1381 taaagataat aacgattatt taagatttat tgaaatgctt aaattttatc aatacaaaag
     1441 aaattgtagt ttttcctatt atctaaaaac aaaggaaata cctcagaaaa atttggataa
     1501 aataggagaa aaaataataa gaattatttc ttattgttta atgcccacac atatccacct
     1561 tattctttct tctgaagtag aagatggtat aacaaaattt atgaaaaatt tacttgatag
     1621 ttatacaaga tatttcaata caaaaacaaa aagaaaaggg cctttgtggg aaagcagatt
     1681 taaaagtgtc ttacttgaaa cagatgaaca attgtttcat ttaacaagat atatacatct
     1741 aaacccggta acagcatatt tgataaataa accagaagaa tggcagtact cagcttattc
     1801 agaatattta gatagtataa aacaggagaa aaaaatttgt gaatatgata atttattaga
     1861 aataaaccca gaaaaataca gggaatttgt agagaataga atttcatata aaagagaact
     1921 tgcaaaaatt aaaaagatag tttttgattg atgtaagtta ctgaaattca ataacttgtt
     1981 aacttcgtaa gttaacaaaa ggggaaaatg aaattatcag tgattattcc tgtttataat
     2041 gaaagagaca caataaacaa aataattgaa aaagttagaa aagtcccaat tgaaaaagaa
     2101 ataattattg ttgatgatgg aagtacagat ggaacgcaaa atatattaaa aaattttaat
     2161 aataaagata taaaagtcat attaaaagaa aaaaatgaag gcaagggaat gtctataaga
     2221 gaaggactta aatatgttag tggagatatt gtggtaattc aagatgctga tttagagtat
     2281 gaaccgatgg attttttaaa aatgattgaa gtaatgaaaa gaaataatgc tgaagttgtg
     2341 tatggttcaa gaattcttgg aaaaggtaaa aaatcatctt tcttctttta tttaggaggc
     2401 aggttccttt cttgtattgt aaatatactt tataatgcaa aaataactga tgagccaact
     2461 tgttataaaa tgttcagaac agatgttatt aagaagataa atttgaaatg cactggtttt
     2521 gaattttgtc cagaagtaac cgcaaaagtt agaaaaatgg gctataaaat ttttgaggtt
     2581 cctattcact atataccaag aagtataaag gaagggaaaa aaataaggtt gaaagatgga
     2641 attattgcta catggacact tataaaatac aaatttattg attaaaatta gtttaaccgt
     2701 ataaattcaa cccccgcggt tataaaaatg gtaaaatgtc caatttgtaa aagtagaagt
     2761 cggttaatat ggaaagaaaa aaaacataag gtttttagat gtaaaaattg taaaattgct
     2821 tttttatatc caataccaga aagtagtgaa aaaatatata acagaaaata ctttgaaaat
     2881 tggtatttga gatattatga tgaaaggaaa agatattttg aaaaattatt taataataaa
     2941 ttaaaaaatt acttgcctga gcaaggaaaa atacttgatg ttggctgtgg agttgggatt
     3001 tttttagata tagtgaaaga aaaaaattat gatgtttatg ggcaggatat ttctccattt
     3061 gctgttgaat attgtagaaa aaaaggtttt aatatttttg acaaaccatt gccagagata
     3121 agtttacctg aaaacttttt tgatattatt acaatatttg atgtaattgc tcatttagga
     3181 aatcctgttg aatatattaa aatatgtaaa agaattttaa aaccaggggg tattttaata
     3241 atcaaaactc cttaccaacc aaattatctt ttctttcttg ctaatatttt atcgtttaca
     3301 ggaaaaagta aagggctatt acatattccc gctcagattt tccattttag ttataaaaca
     3361 tttgaatata tgagcaaaaa ctttaacttt tcaaaaattt ttatgtttaa tattagagaa
     3421 tttttcccag atattatttc tatttctctt ttgaaaaaat ttgttttaaa tgaaaagtca
     3481 ataattttta ttgctaaaaa atgaataata atagaattag attgtttttt ttgataatta
     3541 taatatgtat tggaattttt ttaagaattt ttcaattggg gaaattgtgt ttctggtgtg
     3601 atgagtttct tgcaattttc tgtggatggc aaccattaaa ggatatgata aattatatta
     3661 cctataagga tgctcatcca cctttatttt atgctcttgt tcattttctt ttaaaatatg
     3721 gaaattcaga attttattta agattattac catctttctt tggaattata acaattcctc
     3781 ttgcatattt tgttgggaaa gaatttaagg acgaaaagac aggaattatt ttttcaatat
     3841 ttatttcatt aaatcctgca ttgattttat ggtcaagaat tttaaaatca tactcgcttt
     3901 ttactttttt ccttttactt tctttatttt tctttcttaa aattttgaaa acggataaaa
     3961 aacaatatta tatttttctt ttcttttcag atattattct tctttattta cataattttg
     4021 cgtttatctg gattttaatt gaagttttta ctttattatt caataaaata tttaataaaa
     4081 aatttctttt ttattatatt tttattttta ttttttatct accatggttt ttaaaaattc
     4141 cttatcaatt aaaatttact cttggtgtta taaggcctat cccatttgtt ttaagatacc
     4201 cttatttatt tttttatttc ttttttggtg aaacacttca cccttttaat ttgaaaattg
     4261 tcataccttt tttgatagtt attattttta ttgttgtaaa ttcatttatt gatttaaaga
     4321 aattagaaaa ggttaaaaaa tatcttcttt ttttaggtat tttaatacca ctttttcttg
     4381 ttcctttccc ttcaactgtt cctcaaaatc ttcttccttt ttcaatattt gtttttttat
     4441 tattatcaat tggatataaa aatacaaatt ttacaaaact cctttttttc tgttttttta
     4501 ttttatcttc tttctcaaca tatttttact ttaccggtaa tacaaaaaat tttcatgatg
     4561 taagcaaact tattccatat agagaaataa atgaaatatt ttcagaaaaa ttagatgaaa
     4621 atagtattat ttttttaaat gggacagacc aagattccat gtataaaaaa ttttcacctt
     4681 ttcattggta ttataaagga aatacaaaag ttattgaagt tgcaaaaagg ccaggttcaa
     4741 tagctgaaat aaaaaaaata tgtaaaagtt ataataaaat tggacttttt cttaattata
     4801 atgaagacca ggagtggaat aatgaactga aaaatttctt ttttgaaaat ttctttccag
     4861 tatatcaggc aaaatttctt cataatgaga aacttttaag tcgcctaaaa ggaaagaaag
     4921 aatattattg gtttgttgaa atttatattt ttgagaaaca taagaaatga aataaaatat
     4981 atacaatgaa aaggatactt ataacaggtg gagttggttt tattggtata aatttggcga
     5041 aaaatctttt atgtgaaaaa gataattttg ttgttctcta tgataatctt tcaagaaaag
     5101 gagttgagaa taatcttgaa tggattttat cttttaattt tccaaattta aaattcataa
     5161 aaggagatat tagggattat aaaaagttaa aagaaattgt aaaaaatttt gatgaggttt
     5221 atcatcttgc agcacaggtt gcagttacaa gttctgttga aaatccaatt gatgactttg
     5281 aaataaatgc acttgggact ttatatcttc ttgaagtaat aagaaaaaat tcaccagatg
     5341 cagctgttat ttttagttcc acaaataaag tttatggaga acttctaaat ttaaaattaa
     5401 aagaagggga aaaaagatat tatttaatta agggcaaaga aggaattgat gaaaaccaaa
     5461 atcttgattt tcactctcct tatggatgta gtaaaggaac agcagaccag tatataagag
     5521 attattacag aatttatgga ttaaaaactg ttgtgtttcg tcaatcatgt atctgtggtc
     5581 aacagcagta tggaaatgaa gaccagggat gggttgctca ttttataata aaagcaatag
     5641 aaggagaaaa gataagtatt tatggtgatg gaaaacaggt aagggatatt cttgaagttg
     5701 atgatttaat ttctgcctat aaaattgctg taaaaaatat aaaaaagaca aaaggggaaa
     5761 tttataatat tggtggtggg aaggaaaata ctttttcgct aattgaatta attgaatatc
     5821 ttgaaaaagt tttgagaagg aaaataactt atgatttcta tgattggaga ccgggagacc
     5881 agaaaatttt tataagtaat aataaaaaat ttatggaaga aacaggatgg agaataaaaa
     5941 taaataaatt tgaaggtgta aatagattga taaaatgggt tgaaaaaaat catgagttaa
     6001 ttaaaaaagt aagagaaaaa aaatgagaaa tattggagtt ataggtgcag gacatgttgg
     6061 acttgtaaca ggtgcttgtt ttgcaaaaat gggaaatatt gttatttgtt gtgataatga
     6121 cattgaaaaa attaaaaaac tcaaaaattt taaaatacca ttttatgaac cacatcttga
     6181 agaaattgtg aaagaaactt ttaaaaataa aaagttgact tttacaactt caattaagtc
     6241 cttagtagaa aaatcagaga taatttttat agcagtagga acaccttcta ttgaaagtgg
     6301 aagagcagac ctatcttctg ttgaaaatgt cacaattgaa atagcaaatg ccttatcatc
     6361 tattaaaaag caaaaaggac aaaattataa aattatagtg gaaaagagta ctgttcctgt
     6421 tttgacaggt gaatgggtaa aaaaaacact aaatctcctt tctccaacag gaattgaatt
     6481 tgatattgct gcaaatccag aatttttaag agagggaagt gctgtatatg atttttttca
     6541 tcctgataga attgtgattg gaattgaaaa tgaaagaccc aaaaaaattt ttgaagaaat
     6601 atataaacaa ataaaatgtc taaaattatt tacagatata aaaagtgctg aactaattaa
     6661 acacgcttct aattcttttc tggctttaaa aatttcttat ataaatgcaa tttctcaaat
     6721 atgtgaaagg gttggagcag atgttgaaat ggttgcagaa ggtatgggac ttgataaaag
     6781 aataggaaaa caatttctca aagcaggcgt tggttatggg ggtagttgtt ttccaaagga
     6841 tgtttctgct tttatttcac tttcagaaga aattggatat tcttttaccc tattaaaaga
     6901 agttcaaaaa attaatcagc aacaaagaca attggtagtt aaaaaagcaa gaaatttatt
     6961 atggaatcta aaagggaaaa acattggaat tttaggactt gcctttaaac caaatacaga
     7021 tgatgttaga gaaagttcag caattgaaat aattcaactt ttaaaaaaag aaggagcaaa
     7081 tataaagtgt tatgaccctc aggcatctga caatatgaaa aagataatcc ctgatataat
     7141 atattgtaaa aatccttatg atgttgcaaa agaaagtaat cttcttatat ttttgactga
     7201 atgggatgag tttaaaaaac ttaatttcaa gaaattcaaa gaaataatga aaactcctta
     7261 cataattgat ggcagaaatt ttcttgatta taagaatatc ttaaagtgtg gttttatcta
     7321 tacaggaata ggaagaaaga taaatgaaaa ttcttattag cggaggtagt gggtttattg
     7381 gttcccatgt ttgtgattat tttttaaaag aagggggaaa tgtctggtgt attgataatt
     7441 ttttaacatc aaatatagaa aatattgaga attgtttaaa taacaaaaat tttgttttta
     7501 tagaggatga tatttgtaaa ataaatgttg ataaattaaa aaacaatttt gattatgttt
     7561 tacattttgc gagccctgct tcccctgttg attataataa atatcctgtt gaaacattaa
     7621 gagttaattc aattggaact gaaaatatga taaaaatctc tcttgccaat aattcaattt
     7681 ttctttattc ttcaacatca gaggtctatg gagaccctga aatacctgtt caaaatgaag
     7741 aatactgggg aaaagtaaat ccaataggag aaagaagtat gtatgacgaa ggaaaaagat
     7801 atggagaagc acttattatg gcttaccata gaaaatataa gttaaatagt agaattgtaa
     7861 ggattttcaa tacatatggt ccacgaatgc gactaaacga tggtagagta attcccaatt
     7921 ttattgtaag cgcattaact ggaaaaccaa ttacaattta tggggatggg aatcaaacaa
     7981 gaagtttctg ttatatatca gacctcatca aaggaattat tagaattttg aaatgtaatt
     8041 ataatttacc aataaatatt ggtagttgtg aagaatttac aataaaacaa ctggcaaaaa
     8101 aagtaaaaga aataacaggt agtaaagtag aaattatata taaaccccca ttgcctgatg
     8161 acccaaaaag gagaaaacct gatataacaa aagcaaaaaa aatattgaaa tgggaacctg
     8221 ttgtaacact tgaaaatgga ttaaaagaaa caattccata ttttgaaaag aaactaaaga
     8281 aatgaaaaaa atacttgtaa caggtgggtg tggttttatt ggaagtcatt tagttgataa
     8341 acttattgaa cttggatatg aagttattat ttttgataat ttaacaaaac aggttcatcc
     8401 ctctggtatt cctgaatatt taaatttaaa aagtacattt attagaggtg atgtaagaaa
     8461 cagaaataaa ttgaaagatg ttgttaaaaa aattgatata atttatcatt ttgcttccgc
     8521 cgttggagtt gggcagagtc aatatgaaat ttcaaaatat gtagatgtta atataagagg
     8581 aactgctaat ttactggata ttcttgtaaa tgaaaaacat aatgtaagaa aactcataat
     8641 tgcctcttca atgagtattt atggagaggg aatgtataaa tgtaaaaaat gtggaaaagt
     8701 aaaaccagaa ataagaaatt ttgataataa aaaaattgat gattgggaac caaaatgccc
     8761 aaattgtaaa ggagatatta cagcaattcc aactgacgaa gaaacaccaa caaaaagcaa
     8821 ctcaatttat gctataacaa aaaaagaaca ggaggaaatg tctcttttaa ttggaaaaat
     8881 atatggaatt cctgtggttt ctttaaggtt ttttaatgtc tatggtccaa ggcaaacact
     8941 gtcaaatcct tatactggtg tttgtgcaat ttttttgagt agaattaaaa acaataaacc
     9001 tcccataata tttgaagatg gaatgcaaac aagggatttt atctgggtgg atgatattat
     9061 aaatgcatgt attctttcta ttcaaaaaga ccaggcaaat tacgaaattt ttaatgtcgg
     9121 ttctggaaag ccaacatatt taaaagaaat tgcagaaatt ttaatcaaat tgcttaataa
     9181 aaatttaaag ccagaaataa catataaatt tagaaaaggt gatgtgagac attgttatgc
     9241 cgacataagt aaaataaaag aaaagttggg atttaaaata gaaacagaac ttgaagaagg
     9301 aatgaaaaaa ttaattgact ggtcaaaaaa agaaaagaaa gtaaaagata aatttgatta
     9361 tgcagaaaaa caacttaaaa acaggcacct tattgaatag gattgaatta acaatagtaa
     9421 tcccggcaag aaatgaagaa agtaatttag aaagaacggt tgaagacata gtgcaatata
     9481 tagatacaaa tacaacagaa attattattg ttaatgacca ttcaaccgac aaaacagaac
     9541 aaataggtca acaactatct ttgaaatact cttttatcaa agttataaat aataaaaacg
     9601 aacctggatt ctctacaact ttactaactg ggttcaagca ggcaaaaggt gaatatgttc
     9661 ttcctgtaat ggcagatatg tgcgatgacc caggcagtat tcaaaaaatg ttagaaaaag
     9721 caaaagatag ttatgatatt gtttgtggct caagatatac aaaaggtggt aaaaaaattg
     9781 gtggaccaaa actacagaat tttttttcat tttttgtttg taaatcattg cgttatttaa
     9841 tcaatttacc cacaaatgat gtttctaatg cttttaaatt atataaaaaa aatattttca
     9901 atattataaa accaaaagaa aagggatttg ctatttcaat ggaaattact ctaaaaagtt
     9961 atttttatgg attgaaaatt actgaaattc ctacaacctg gtatggaaga aaaaaaggaa
    10021 aatctaaatt taaacttata aaaacatttc cttatgttaa actttatttc tgggcaattc
    10081 taaaaaaatg gaaatttcta taataattcc ttcttataat gaagaaaaac gtattggaaa
    10141 atcattagaa aaaatatgga agtactttaa aaataaagga tttccatttg aaataattgt
    10201 tgttgatgat ggaagtactg ataaaacagt tgaaattgtt gaaaagttta aagaagggaa
    10261 aaaagaaata aggatattga agcatgataa aaataaagga aaaggggcag cagtaagaac
    10321 gggagtgata aattcaaaag gtaatttaat tttatttact gatgcagacc tgtcaacacc
    10381 cattgaagaa tttgagaaac ttaaaaaagc aattgacgat ggttatgata ttgcaattgg
    10441 ctctcgagga cttcctgaat caaaaataat tattcctcaa ccatggtata gaagatattt
    10501 agggaaaatt tttccattat tggtaagaat tttagtaaca aataaattca aggatactca
    10561 atgtggattt aaattattta aaagagcggt agcaaagaaa ttgtttgatg aattaaaaac
    10621 aaatggtttt gcctttgacg tagaaattct gtataatagc ataaaaatgg gatataaaat
    10681 aaaagaagtt ggtgtaattt ggagcaattc ttttttttca agtgttgcaa tatttaaaga
    10741 cccattgaaa atgttcattt ctcttttaaa aataaggagt ttcaaataat gaaagtactt
    10801 tttacctaca taccacaacc gttttctaaa aagggttatt tattagtaag tcagaataga
    10861 ttcactaaat ggcgaggaac aaaagaactt atatatcctt taattcctgc ttctggttta
    10921 acacttttag atttaaaagg atatgaagtt tattatttag attgtatctt tgaagaaatt
    10981 gatgaaaaaa atttttttga atatattgaa aatcttaaac cagatattat ttttactgag
    11041 acaaaaactc ctataataaa atatcattgg attatttctg aaaaaataaa aaggaaattt
    11101 ccccagatta ttacttgtat tataggagac catatcactg ttttaccaga agaaacaatg
    11161 aaaaatagta aatttgattt tgttttaaca ggaggagact ttgattttta tatgttacaa
    11221 cttgtccaat ttttatctgg taaagaaaag attccatcag gtttgtggta tagagaaaaa
    11281 aatgggaaaa ttaaaaatac tggaaaattt attttgattg ataatcttga ttctttacca
    11341 tttattaata gggaaattgt cccatggaaa aattatcatg aagcatggag aatttctgaa
    11401 aattttatgt atctttcagg tagtagagga tgtccttata gatgtacttt ttgctcctgg
    11461 ccccaaatgt tatttgataa taaaattaga tatagaagtc ctgaaaacat agttaatgag
    11521 atagaatttt tggtaaagaa atataaaaca gaagaatttt tctttgatga tgatacattt
    11581 acatttaata aaaaatggtg tattgaaata tgtgaagaaa taattaaaag aaacttaaaa
    11641 ataatgtgga gttgtaatgg aagagttgat aatgttgatg aatttttatt aaaaaagatg
    11701 aaagatgctg gatgccgtct tataaaatat ggtgttgaaa gttattctca atatactctt
    11761 aataaaataa agaaaggtta tacaatccaa caggttaaaa aagcatttag tttaactaaa
    11821 aagagaaaaa ttcttattca tgccacagca atgattggtt tcccatggga aacaagaaaa
    11881 gatattttaa atactatcgg atttataaaa agtttaaaac cagatacctg ccaattttca
    11941 attccaataa catatccagg gacggaatta ttcaaagagg cagaagaaaa aaactggctg
    12001 aaatttaatt ataattggga gaaatatgat atgagtttac caactttaaa aaatctttat
    12061 ctatctgatg ataaattagt aaaattatgc aaattagcat gggggaaaat ttatttggat
    12121 ccattattta ttttaagaaa agttttacgg ataagaacaa ttagcgaagt aaaatggtta
    12181 atcagaggct ttatttcatt tctttttggc catgtcaaac caattcaaaa atgaaaactt
    12241 attttgtaat aataattcct tgtaaaggtt ataatgaata tcttaataaa acgattaaag
    12301 gatgccttaa tcaaactttt aaagatttta aaatttattt gttgccagat aataattttg
    12361 aaaataatat tttcaaaaat gataaaattg taattaaatc aacaggtaat tattttcccg
    12421 ggaaaaaaag aaatatagga attgaaattt ctgaagcaga agtatatgct tttatagatt
    12481 cagatgcaat tcctgaaaga gtatggttag aaaaagctta taattattta aaagacgaaa
    12541 aaataaaaat tataggtggg ccgaacttga ctcctgcgga atctaattta agagaaaaaa
    12601 tatctggaat tttactttca aattttttag ttactggaaa gttttctatt agatataaaa
    12661 aaaatctaaa attagagacc gttttggaat tacctctatg taatttattt ataaaaaatg
    12721 aggtttttaa atccgttggt aaatttaaag aaaatacatt aacagcagaa gatgctgatt
    12781 tttgttttag ggcaaaaaaa cttggaattg atacaaaata ttcttcagaa atagttgttt
    12841 atcacaaaag aaggcctata tttctgcctt atttaaatca aatttttcag tatgcatttg
    12901 ataaaggata tagaattaaa aaaaattttg ttccaaaata tctatattat tttttaccat
    12961 catttttttt aatttttttg ttaattggct tgtattcttt aatttttttt aaaaattttt
    13021 tattttttta ttgcttcttt atatctttat atttttttct cgtttttatt tcgtgtttaa
    13081 taaattggaa atatcttttt tatatattta gtgggattat tttaacccat ttagtttacg
    13141 gaagtggttt tataattgga ttattttcaa aaagagaaaa tgaaaattaa agaattcgta
    13201 gttttattta taatttcatc tatttcctat ttttgtggat tgaattgggg gttaccatct
    13261 aaaagtttaa ataaactata ttttaatgat atagaaacaa tagaaaaaca tatagaacaa
    13321 ataaaaaaat atcctattga aaaaatttat aaaggaatgg ggccatatct tgcaaaatat
    13381 ccagaagtag aaaaagaaaa attacctcgt ttttattaca atcctattag aagttatcat
    13441 ccagatgagt attatgtaat aaaaagtttt tcttctatga aaccagaaaa atttgatttt
    13501 aatcctcatg cttattcaat cggtggtgct tatttatatc ttgtaggcct attccttttt
    13561 ttattttcca aattaaattt tgttgtgctt tcaaaagata tatgttttta tttcttacat
    13621 ccagaagaaa ttggtaaatt ttatattatt ggaagagcaa tcactgctat ttatggaatt
    13681 ggcattatac ttttaagtta tttattatgt aaaaaatttt ttaaggatgg attgaaaagt
    13741 tttttaattt gtcttttaat tttattttct cccttaataa ttttaaattc tcactatatg
    13801 tatgttgata ttccaggttt attctggata actgcctgtt tattcataac aagcaattcc
    13861 atagaaaaat tctcatttaa aaatgtattc ttagcaggta tttttgcagg acttgcatgt
    13921 ggtacaaaaa ttacttttgt tgtatcaata gtaattccat tacttggaat ttttattatt
    13981 tttaaaaatc ctaaggatat tgtaaaaaat ttcttattag ttgttatatg ctttattttt
    14041 tcttttttta taaccaatcc atattttttc ttaacttttc ctgaaccctt aattgaatta
    14101 gaacagcaca ctggactttc ttttaatggg aaattttact ttttaagttt aaaatatggt
    14161 ttagggtttc cacttcttat ttttgcttta actggaactt tgttgagtat tttttttatt
    14221 aaagaaagaa gtgatcttga gaaaaaatta ttatttttac tttttagttg gacagttttc
    14281 ttctttattt ttatttctat tttctcaaag aattttgcaa gatacatttt acctttagtc
    14341 ccttctttta ttattcttgg aggatatgga tggtttttga cattaactaa aacaaaaaaa
    14401 attttaaaat ttctgatttc tttatggatt atttttgtta tattatttac ttttttttat
    14461 gggatgagtt atgagatgtt atttatacaa aaaaatataa gaacagaagc aggtgaatgg
    14521 ataaaggaaa atattcctgc tggaagtagt attggagtta cagaagttcc atggcaattt
    14581 cagatgccac cttttgatta tttcaaatac aatcttattg ttacaggata tgatttcaac
    14641 aaattaaaaa aagaaaagcc agaatatttt ataatctctt catttcaagc acctattcca
    14701 ccatttccat taaaattaca gatagaaaaa aatagttttt ggaatgaatt tataaattca
    14761 tctatctata aagaagagaa aaaatttcaa aaatttcctt cgtttctatc ttttaaattc
    14821 caatttaatg aattacctga agatttaatt tatttaaacc caacaattgt aatttttaaa
    14881 tataaaaatg agacctttta aattcaattt accaaaagaa ttgatttctc aatatccatt
    14941 aaaagagaga gagaaagcaa agttaatggt agtaaataga aaaaatggaa aaataattca
    15001 tgacatattt gaaaatattg ataaatacat gagtgaagaa gatgttcttg ttttaaataa
    15061 cacaaaagtt attccagcaa gattattatg tagaaaagaa acgggtggga aaattgaaat
    15121 ttttcttctt aaaaaaatag aaaaaaataa gtggaatgtt ctggtaggtg gaaaaggaaa
    15181 aataggtagt aaattttata aagaagacat tgcagggaaa attattgaaa aaaaagcaga
    15241 tggcagtttt attgttgaat ttaatattga agatgaaaat gaaattaaaa aatatgggga
    15301 agttccactt cctccatata ttaaaagaaa agcagaagaa attgacaaaa tttattatca
    15361 gacagtttat gctgaaaaag atggttcaat tgctgcccct actgctggat tacatttcac
    15421 agaacaactc cttgaaaaaa ttcaaaaaaa aggtataaag gttgtttata ttacacttca
    15481 tatgggatgg cattcaataa aagtttttaa ggaagataat acagaaaaaa atgtttcaac
    15541 agaatttttt gaaatttctc aacaaacagc agagatacta aataaatcaa aaaaaaacaa
    15601 aaaaaatata attgccattg ggacagggac tgtgagatgt ttagaaagtt cagtagaaaa
    15661 tggaattata ataaggaaaa agggctttac tgaactattt attaaacctg gttataaatt
    15721 taaagttgta gataaactca ttacaaattt tcatcttcct gattcaacgc atctttattt
    15781 agtaagtgct tttacaggtc ttgatttaat agaaaaagca tataatattg cagtagaaaa
    15841 aaaatataga ttttattctt atggtgatag tatgttcatc atctaaaagt agagagtaac
    15901 aagattgaga aagataaatt cacggtatct tgcggataga aagcaaggaa aatagatttt
    15961 ctctttgtaa aagaggtttt cccctttaac aaaccgacta gggaagcaag tggtagcaaa
    16021 gcagagttgg tgcttgtttc ccctctttat aaaagagggg caaggggaga ttttcaacaa
    16081 gcacaacgga agtcagaaag gatttcgggg agatttttaa atccatccca tccttccctt
    16141 attaatgtaa ggagtaagtt ctccctttat aaaagggaga ttaagaggga tttaataggg
    16201 ggttccaagt ccaaaggatc cttcggacac aatgggagtt agagggaatc tcttacttaa
    16261 attaaaaaga agaggagaaa tatgtttgag tttaaaatta taaacaaaga taaaaaaaca
    16321 agagcaagag ttggagaatt tttaactgag tatgggaaag ttgaaacacc aaattttatg
    16381 ccagtcgcta cattagggag cgttaaaaca cttacttctg aagatttaat taatatggga
    16441 gtagatattt taatttcaaa tgcctatcat ctttatttaa aaccgggtat agatattatt
    16501 aaaaaaagtg gatgtataca taaatttata aattggaata aatcaattat aactgatagt
    16561 ggtggatttc aaatattcag tttagaaaat gtaaaaatta cagaagatgg tgttattttt
    16621 aaatcaaaat tagatggttc aactcatttt ataactcctg aaaaatcaat tgaaattcaa
    16681 aataaaatcg gtgcagatat tataatgagt tttgattatt gccctaaaaa ttggaatgat
    16741 tatggagaag ttgaaaaaag tgttaattat acaattaaat gggcagaaag atgtaaaaaa
    16801 tatcacaaaa acaaagaaca aacattattt ggaattattc agggaggagt tcataatgac
    16861 ctgagggaaa aatgcataaa taaacttgaa atcttaaatt ttgatggata tggaattgga
    16921 ggattatgcc ttggagaacc agaggaaaag acatttgaaa ttgcagattt tatttcaaaa
    16981 aaagtaaatg ataataaact aaaatatttt atgggtatag gaatgccatt acaaatcctt
    17041 aatttagttg aaatgggata tgaccttttt gattgtggaa tgataactca tattgcaaga
    17101 accggctcaa caattacttc aagagggaaa ataaatataa aggggggaaa gtacaaagat
    17161 gattttatac ctcttgaccc tgaatgcgat tgttttgttt gcaaaaatta tacaagagca
    17221 tacataagac atcttataaa ttctaaagaa attactggtt taagattaaa ctcatatcat
    17281 aatgtttatt ttataataaa tctcttaaaa aatattagaa agagtataaa agaaggtaaa
    17341 tttagtgatt ttaaagaaaa atttgaaaaa aaatataaag aaaaaaattg acatttcttt
    17401 tataatataa tacattatta ctatattgat aaatagaatt aacttttagt actaaaaata
    17461 aggaggaatt atgaaatttt taatgttagc acaggcacag caacaatcta caccatcttt
    17521 attaggtgct ttattaccac taattttaat attctttgtt ttttattttc tgcttatatt
    17581 accgcaacaa aaaaaacaaa aactacataa aaaaatgtta gatgatttaa aagaaggaga
    17641 taaaattgtt actgttggag gattaattgg agttgtttca aaaataaaag ataatattgt
    17701 aacaattgat tgtggtaatg gggtaaaagt tgatttttta agaaacgcta tttcacaggt
    17761 aattaaacca cagaatgata ataagtaaga caggatattt tgaagttttt aatggatgtg
    17821 caggagatat gattgtcgga tgtctaattg atgcaggagt taatccatca attctaaaaa
    17881 atgaactgca aaaaatccct atttctaact acacaataaa aataaaaaaa gtaaaaagaa
    17941 agacaaattc ttcccatttt atatctgcaa cacaatttat tgttatttta aaagaaaaag
    18001 agaatcaaag aaaatatact gaaattgtca atttaataga gaaaagttca ctttcagatg
    18061 atataaaaag gaaaacctta aaaattttta atattcttgc aataagtgaa agtaaagttc
    18121 ataaagaaaa aattgataat gtacattttc atcaaatagg acaaacagat gctattattg
    18181 aaattgcaag tgcagtaata ggactgaaat tactgaatat agaaagtgtt tattcaagtt
    18241 caataggaat ttcaatgcca tctcctgcta cacttgaaat tctaaaagga cttcccgttt
    18301 taataaaaca tattccttat gaaatttcta ctccaactgg tgtttcaatt ttaaaaggat
    18361 tatgtaaatt ccatgaaata gatgaaaaaa tttcaataga aggatatgga tatggcacag
    18421 gaacaagtga gacactgcct tccgatataa tgaagttcat atatggaaaa gaaattgaca
    18481 aaaaaaaaga agaaatttac atcattgaaa caaatattga tgatatgaac cctgtaatat
    18541 ttgagtattt atacgaaaaa ctttttcagg caggggcaac cgatgtttct gtttttacag
    18601 gtattgggaa aaaaaatagg cctgttttta accttcaaat tatggtaaat gaagaaaatt
    18661 ttgaaaaagt gaaagaaatt atattttctg aaacaacaac aattggattt agataccaaa
    18721 aagaagatag aataattctt caaagaaaaa ttaaagaaat aaaaacaaaa tggggttctg
    18781 taaagattaa gataagtttt tatggtggta aaatttataa tatttctcct gaatatgatg
    18841 attgtaaaaa aatatccaaa aaattcaaaa tacctttaaa aaatgttata acagaagtga
    18901 ataatttatc aaaaaatatc tctatgtgat ataatgtaaa acctcaacaa aaggcacaaa
    18961 gaaaaatata taaatttaaa attaaaagaa gaaagtgtaa aattataact taaaatttaa
    19021 aaagtttttg gttttttgta tttcattcgc caaaaaacga ggcggatgct aaaatacgcg
    19081 cttttcgctt tacattttgc gttcttaata actattgaaa tacaaaaaag attttaccat
    19141 aagaaaaatg tttgtcggaa caataaaaat tgaactttta attccgggat gcaaaagttt
    19201 gaaagataag agaaacatta taaattcaat taaaaacagg ataaaaacaa aatataatgt
    19261 ttcaattgtt gaagttgact tctatgaaaa atggcaaaga gcagcaattg gtgtttcatt
    19321 tgtaaatggt gaagcaaaat caacagaaat tctatgtcag aaaatacttg atttattttt
    19381 tgataataat gattttattg tattaaataa caaaaattct attatttcta tagaagactg
    19441 agaaagaatg gaaaaacaat acaacccaaa agcaatagaa gaaaaaattt ataatatatg
    19501 ggaatataat aattttttcc attgcgaagc agataagaaa aagaaaaaat atgttatagt
    19561 tattcctcca ccaaatataa ctggattttt acatatggga catgctttaa ataatacaat
    19621 tcaggatata ttgataagat ggaaaagaat ggagggctat aatgccttgt gggttcctgg
    19681 aactgaccat gcaggaatag caacacaaaa tgtagttgaa agaaaacttt taaaggaagg
    19741 aaaaaaaagg caggatattg gaagagaaaa atttattgaa gaagtatgga aatggaaaga
    19801 agaatatggt ttacgaatta ttgaccagtt gaaaaaatta ggtgcatctt gtgattggaa
    19861 caggttaaga tttacaatgg atgaaaattt atcaaaggca gtaaaatatg ctttttacca
    19921 actttataaa aaggaactta tttatagagg aagaaaaatt ataaactggt gtccaagatg
    19981 cacaactgcc ctttcagacg aagaagttga atataaagaa attccttctt ttctctatta
    20041 tataaaatat cctctaattg aaggcgattt tattgaagtt gcaactacaa ggccagaaac
    20101 aatgcttgga gatacagcag ttgcagttaa tcctcttgat aaaaggtata aaaaccttat
    20161 agggaaaaaa gtaaaacttc catttgttga tagagtaata ccaattattg ctgatgaaaa
    20221 agttgaagta aaatttggaa caggggcagt gaaagttaca cctgcccatg acccgactga
    20281 ttttgaaata gggcaaagaa ataaattacc ctttgtaatt gttatagatg aagaaggaaa
    20341 aatgaacgaa gaagcaaaac aatttaatgg tatagataga tttcttgcaa gagaaaaaat
    20401 tattgagcaa cttaaaaata atggtttggt aagtaaaata gaaccatata tccatagagt
    20461 tggacattgt tatagatgtt taaccccgat agaaccatat atttcaactc agtggtttgt
    20521 aaatatgaaa ccactttcaa aacccgcaat tgaggttgca ttggaaaaaa aactaaaatt
    20581 ttatccagaa aaatgggaaa aaatttatct taactggctt tataatatta aagactggtg
    20641 cataagcaga caaatatggt ggggacatag aatacccatc tggtattgtg aaaataaaga
    20701 ttgtccacct attgtctcta ttgaagataa tgttaaaaaa tgccctaatt gtggaagtga
    20761 aaatataaag caagatgaag atgttttaga tacatggttt tcatcatggt tatggccatt
    20821 ttctgttttt aattggcctg aaaagacaga cgatattgat gttttttatc caactgatac
    20881 tcttgtaact gcacaggaaa tacttttctt ctgggttgca aggatggtaa tggctggaat
    20941 ggaatttatg aaagaaatac cttttgaaaa tgtttttata catggaacag taagagatga
    21001 aacagggaaa aaaatgagca aatcattggg aaatgccata gaccctgttg atataattga
    21061 aaattatggt gcagatagtt taagatttag tttaatttca atgacatctt ttggacagga
    21121 tgtatatctt tctccaactt tttatttaaa aggaagaaat tttatgaata aaatctggaa
    21181 tgcaggaaga tatatcataa caataaatga agaaatggga acaaatgata taaaaattta
    21241 tgaaaatatt gattttaata acttgaaatt tccagaaaaa tggctcattt taaatcttaa
    21301 tgaaacagta aaaaatgtta cttccctatt ggataaattt cattttaacg aaagtataaa
    21361 ttatttatat gattttttct ggcatacatt ctgtgattgg tatattgaaa taagtaaaat
    21421 atataaaaaa gaaaatgatt attttaaaaa tgttgttatt cctatcttga ataaatccct
    21481 tattatttca ttgaaacttt tacatccata tataccattt ataaccgaac agatatggtt
    21541 atctcttaaa aaatacattt ctgaccttga atctgactat ataattatta gtaaatggcc
    21601 tgttgttgaa aattatagag acgatgacac aattgaaaaa atgaacagga taattgaaat
    21661 tattacaaat ataaggacta taaaaacaaa aataaaagtt cctgtcacaa aaattattga
    21721 ttgttatata gaaggagaaa tagatgatat tgaaaaagaa atagttgaat cactcggaaa
    21781 ggtaaaaatt aaagaaaaac aggacattat gaaaaataaa aattatcttc taaaaaatct
    21841 ttcatcaggt ggacttttta tatcaattga aggtataata gatatccctg aagaaattaa
    21901 aaagttagag ggaaaaaaga aagaatttga gatattactt gaaaaaacag agaaaacatt
    21961 atcaaataaa caattccttg aaaaagcaaa aaaggaagta attgaaaaaa caataaataa
    22021 taaaaaagac cttgaagaaa aaattgaaaa tataaaaaaa gatattgaat ttttcaaggg
    22081 acaaatagaa taacataaat ctttataaaa aggagagaga aaatgtatgg atttatagat
    22141 attggtggga aacaatataa agtagaaaaa gggaaaaaat ttgatgtttt taatttaaat
    22201 aaaaaagtag aagatatagt agaatttaat ccagtttgtt tttttgatga taaagattta
    22261 attacagaag aagataaact aaaaaatatt agtattatct gtaaagttct tgaagaaaaa
    22321 aaaggggaaa aaatttatgt cttcaagaaa aaagcaaaga caggttataa aaagggatat
    22381 ggacatagag accatattac tgtattaaaa gttgaagata taaaaataaa gtgaaagaac
    22441 atattaaggt aataagagat gacaatttat caggtgaaag agtcctaata attgctttta
    22501 aaaagaaaga cccaatagaa acagatacct ctgttagaga attggaattc ctctcaaaaa
    22561 cgctcagatt aaatatcgta aaaacactcc tcttaaaaga aaatttaata catccatcta
    22621 cttatattgg gaaagggaaa ttatttgaaa taaagaaaat tgtagatgaa tacaaaatca
    22681 cattggttgt ttttgaaaaa gacctttctc ctgtccaaca aagaaatatt gaggaaactt
    22741 taaatataaa tgtaatagat agaactctac tgatacttta tatatttgct gaacatgcta
    22801 aatcaaagga agggaaaatt caagttgaac tcgctcaatt aacttatctt ttaccaagat
    22861 taacagggca tggaattgag ctttcaagaa ttagaggtgg gcttggaata aaaggtcctg
    22921 gagaaatgaa aattgaggtc tacagaagaa gaataaaaga tagaattgct aaattaaaac
    22981 aggaaataaa agaaatagaa aaacatagaa atgttttaag aagttcaaaa aaaagagaaa
    23041 aatttcatgc tgtttcactt attggatata caaatgttgg gaaaagcact cttcttaata
    23101 aattaagttc ttcaaatctt tatgttgcta ataaattatt ttctactctt gaccctgcta
    23161 ctcgtcttgt atatatagga gatgataaat atgttttgat gagtgatact gtcggactta
    23221 tttctaatat tccacaccat ttaattgagg cattcaaatc aactcttgaa gaaataaaat
    23281 atgctgacat tcttttatgt atttatgatg tttcaacaaa catagaaaaa caaaaaaata
    23341 ctatctatga tgttttaaaa atacttaatg ttgaggaaaa gttaagaata gatgttttca
    23401 ataaaattga tttacttgaa aaagaagaac ttaatataat gaaaatacaa aaccctgaaa
    23461 aaatttttat ttcttcatta acaaaagaag gttttgacca attaagagaa aaaataatgg
    23521 aggtttttta tggtattggg gttaaataag gatgacattt taaaaatcaa aaaaatatcc
    23581 aatattattg aaaaaaataa aaatttcctt attacaacac atcaaaatcc tgatggagat
    23641 gcaattggtt ctgaacttgc tatgttttta tttcttaaaa agcaaaataa aaatgtaaaa
    23701 ataataaatt gtgataaagt tccttcaatt tataaatttt taccatcagt taaagaaata
    23761 aatattcaaa ataaaaaaaa tttaaaaaac aattttgatg taattattat tattgattgt
    23821 agttcacctg aaagaactat tgatatttca gattataaaa aaaattcact gatagtaaat
    23881 attgaccatc atattacaaa ttttcaattt ggaaatttaa actggattaa ccctgaattt
    23941 tcagcaacag gcgagatgat atattttata atagaaaaac ttaaaaaatt agacaggtca
    24001 attgctgcct gtctttatac tgcaattatt actgatacag gaagatttac ttaccggatg
    24061 agtaaattta caatggatat agtaaaaaat ttaattgaat ataaaatcag tccagttgaa
    24121 attgcaacaa aaatttattt agaaaaacca tataaatcaa ttaaactttt atcattggca
    24181 ttaaacaatc taaaattcga tttcaaaaat aaagtttgct ggatgaaaat tacaagacaa
    24241 ctttataaaa aaacaaatac agatgaagaa ttcacagagg gatttattga ttttctttca
    24301 gaaataaaag aaagtaaaat tgtttttcta atgaaagaaa aagaagatgg cataaaagta
    24361 agtttaagaa gtaaagaaaa gtttgatgtt gaaaaacttg caagaaagtt tggaggaggc
    24421 ggacataaaa aagcatctgg ctgtttcttt aaaaataaga aaatagaaga agtagaaaaa
    24481 acaattttaa atgagataag aaaaaatgga cggaattata aatataaata aacaaaaagg
    24541 aatgacctca tatgatgtta taagatttat aaaaagacag ttcaatttca aagagaaaat
    24601 tggacatgca gggacattag acccaattgc agagggtgtt cttattattc ttattggaaa
    24661 tgcaacaaaa ttaagttgta aatttatgaa atttgaaaaa gaatatattt gtacgatgga
    24721 attgggtaaa aaaacagata cagatgatat tcagggcaaa atcatagaag aaaaagaagt
    24781 aaaagttaca acaaaagaaa ttattgagac aataaaaaat tttgaaggag aatatattca
    24841 aacaccacct gttgtttcag caattaaaca tcagggaaaa ccactctata aattttacag
    24901 agaaggaatt attataaagc caattccaaa accagttata attagagata ttgatattga
    24961 agaaataaaa cttccattta taaaaataaa agttatatgc acaaaaggaa catatgtgag
    25021 gtcgctttgc agggacattg gtgataaatt aggatgtgga gcaactcagg tagaattaaa
    25081 aagaataaga attggaccat tttcaattga aaagacattt actttacaac aattaaaaaa
    25141 aataggtatt gaaaatgcaa ttattacaat tcaaaaactt cagcaaatac ttaaaaatga
    25201 aaaagaaaaa ttttaaaatt agcgttgctg gtattagagg aatataccca gatgaaataa
    25261 caccagaaat tgcttttaaa tttgcatttg cctttggatt ttttggtaaa gggaaaacat
    25321 ttctggttgg aaaagataca agaataagtg gagatgtttt aatttcttct gtaatatcag
    25381 gactgctttc ttctggaaaa aatgtcctaa atattgaaat aactccaact cctatgattg
    25441 gatttataat agagaaaatt gaaaacaaat atggaattat ggtcactgca agtcataatc
    25501 cagaagaata taatggatta aaatttttca ataaaaaggg aaattttata aataatgatg
    25561 aatggaaaaa atttttagaa attataaaaa gtgttaaaaa attaaaatta tcttcaattc
    25621 caggtgaaat taaaaagatt gaaaaaaatt atataaaaat ttattttgat aatatttata
    25681 aattagtaga tgttaagaaa ataagagaaa aaaagtttaa ggttattgtt gatgcctgtc
    25741 agggtgtttc ttctatctat actgaagaat tcttaaaaaa tttgggctgt gatgtattgg
    25801 tcttcaataa atttccatat ggtaaatttt ctcataatcc agaaccactt atagaaaatc
    25861 ttcagcaatt aaaagaaaaa gttattaaag aaaaaggaga tattggtttt attcaagacc
    25921 ctgattgcga ccgtttttca tttgtctgtg aaaatgctga tattccagga gaagaaacaa
    25981 ctttagcaat ctgccttgat tcagtccttg aaaaagaaaa aacacctgtt gttgttaact
    26041 tatcaactac ttctttaatt gatgatatat gtaaaaaaca taaagtcaaa atttatagaa
    26101 caaaaattgg agaaataaat gttgttgaaa aaatgagaaa aataaagagt agagcagggg
    26161 gagaaggcaa cggcggggtt atatggggaa aatttcatta tggaagagat agttacattg
    26221 gaatatccct tttgcttgaa aaaatggcag aagaaaacaa aaaaatatct gaaattgtaa
    26281 ataattatcc taaatattat atgataaaag agaaatataa aattaaaaac ataaaattta
    26341 ttcttaaaaa aataaaaaaa atttataaaa acgagaaatt ggatttaact gatggaatta
    26401 aaattataag agaaaatgga tggattcata taaggcaatc aggaactgaa ccagttgtaa
    26461 gagttatagt agaagcaaca acaaaaataa ctgctgatga atatctaaaa gaaatttata
    26521 gtatactttc cgccagacca ggtatgccag accaggtatg gagaagtact taatggagag
    26581 gacagtcctc ctcaaaaaaa tggaaatttt aaatgaaata ataaaaactt caataatgct
    26641 ttttattgta atagatgcaa ttggtggtat tcctattttt atttctcttg ttccagacat
    26701 ctcagaagat gaagttaaaa aagttgtgaa tcttgctata attgtaagtt tttttattct
    26761 tttatttttt ggagttattg gtagatggat actttactta tttaatataa atttagaaga
    26821 atttaaaatt gcaggtggat ttctccttct tattattgcc cttgacgaaa tttttaatat
    26881 attacctgaa agaaaatatc caaaagaaga acttggaatt gttccaatag gttgtccact
    26941 acttgcaggt ccaggagcaa ttacagtaat tcttgtaatt ctttatagat ttaattttcc
    27001 ccaaaattat attataatga gtgcaagtgt agttattgtc acattggtaa actggattat
    27061 tctttcaagg ttaattaata tcaaaagaat ttttggtaga aaaggtatat tacttcttac
    27121 aaaattgatg ggaataattc ttgctggaat ttcaataaat tttttagtta gtggaataaa
    27181 aaacttattt accggctaat caattttttt gtaaaaatct ttgatttttt ttattgcctc
    27241 ttctggttca ttaacaatag aaaaaattaa aaggtcttca ggagaaatat atcctctttt
    27301 aagttgggta tttttcatcc aatcaattag tcctttccaa tatgaactat caaaaagaat
    27361 aataggaaat ttatgaattc ttcctgtctg aattaaagtt attgcttctg taaattcatc
    27421 aagagtccca aatccacctg gaaaaattac aaaacccttt gtatatttaa caaacataac
    27481 ttttcttgta aaaaaatatt taaatgaaag aagtgttttg atatatgggt ttggcttttg
    27541 ttcatgaggt agttctatat taagccctat tgaatttcct ccagcaagtg ttgctccttt
    27601 atttgctgct tccattattc caggaccccc accagttatt actgtataac cattttttga
    27661 aagtaatttc cccaattcaa ctgccttttt ataccagata tctccttctt taactcttgc
    27721 agcaccccaa acagttactg catctttcac attacctaat tcttcaaatc cctcaataaa
    27781 ttcagccatt attctgaaaa ttctccatga ctcttctgca taaatttttt cttccttatt
    27841 cattatctat ccccctcttt atttattttt tcaactatta gaactggacc atcttttcca
    27901 ataactttta tctcttcatt ttttttaata atttcgtttt tatcttttgg aatacctttc
    27961 caatactcac catgaacaaa aattaatcct tcaggattta aatccgtttt aacccttcct
    28021 ttttctccaa taagtccctc agttccagtt gtaacttttc tccttctcgt tttaagagca
    28081 aataacaata tagcccatat aaaaaaacca gatgcaaaag caacagtgaa aattagtgaa
    28141 gtagcaattt ttatttcaga accaggtctg attagcataa aagaaccaat aagaaaagaa
    28201 ataatacctg aaataaaaaa caatccaaat gttggagtta ttgcctcaat tccaaaaagt
    28261 ataaaaccta aaataagtaa aagaagtcct gcaatgttta taggcattgt atgtaaagca
    28321 aaaaatccaa gtatcaaaca aattgtgcct gcaatacctg gtagtccaaa accaggatgt
    28381 gaaaattcta atattattcc ccacatacca attattaaaa gtaaatacgc aatatttggg
    28441 tctgaaattg tttttaaaaa atcatcttta aatgattgtt ttagtgattc aaaaacaata
    28501 tcttttgtat gaagaataat ttctttttca tttttcttta tttttttccc atcaagtttt
    28561 ttaagaagtt ggtcaatatc ttctgctata aaatcaataa cattattttt taatgcttct
    28621 gtttcggttg aggaaatact ctctctcact gctttttccg cccatttttc atttcttcct
    28681 ttatatttag caatactttt tataaaactc accgtatcat taactacttt ttcctccatt
    28741 gttttgttct gctcatctcc accagttaaa gtaacaggat gagcagcacc tatatttgtt
    28801 gcaggtgcca tcaccaaaat atcacacgaa agagcaataa aagttccagc agaagcacat
    28861 tgtgctcctg atggataaac aaaaccaata attggaactt ttgattttaa tatctcctga
    28921 acaatttttc ttgtagagga tagaagacca ccaggagtat caattattag taaaacaaac
    28981 tctgcattat tcttttctgc tgaatttata actcttgtaa tttgataata tgtaatagga
    29041 ccaatagcat catttatatt taaagtgtat gctttatttt cagaaaaaca aaaggatgat
    29101 attaaaaaaa agcaaggaag aaataaaatt atttttctca tttttctaaa atagcaggtc
    29161 ttttataaat tttcccttta tattcaactt caatcattgg ccagttttta tcttctgtta
    29221 ttattgtatt tttatctttt tcaataataa ttgtatcttc tgattttgtc cctgttattg
    29281 aaggattcca agcaaatgaa cttttttcaa taactttttc atctgtctct tctgtaaccc
    29341 taaaatatct tgttgaataa ccagttggtc ctccttgatg atgtaattgc cactcatttt
    29401 caaaacctgt ttctttatat gatgattttg cttttttaaa aatatctgac atcttcacat
    29461 ctggtcttgt ctcatcaata aaaacagtat caacaaaaca aacactttta tgttttctca
    29521 aaatagattc ctccaacttt ccaaaattaa ctattcttgt aagagataca ataagaccat
    29581 atcttttccc acaaatagca attaaaactc tcttatctaa ttttttttct gttggtattg
    29641 gatgtctgta tttctcaatt cgttcatctg caccaacaag tataacaact ggaattatat
    29701 ctttttccca taacttttga cttactatac ctgcaatttc agtttctttc attcccggtt
    29761 ttataacttt tgaaatttct gtaatacttt cagatgccaa ttttccaact tccttatatc
    29821 tatcaatttc ctcatcaact aatggaaaat gtaatttttc taatttcaca aggacagtaa
    29881 aattagttgg aatatccgaa ccaattttgc ctttacatat ttttttaact tcctcaaaca
    29941 actcattttc tttataccaa ttctttacga taaattccca atcactaatt tcttcttcaa
    30001 aaattcttgg atattcaata tttgttgtta aaagaaaaga tttatcaggt gttattaaaa
    30061 tactacaaac acctttttct gtattaagtc caacaaaatt tttcttccca ccggttaacc
    30121 atgaaaaatt tgatacacct gatactaaaa ttccactcaa tccattttct tttatatatc
    30181 ttcttactct ctcaatttta gtttcttttt ccatttcctt aatttacctt tttttctttt
    30241 ctgttgtcaa atttttagta tgtcagaaat acaaaaagtg ttagtaaatc ctaactgaac
    30301 cccatactaa ttctcagtat ctaaatatct tattgctcaa tagatatacc tacctgaact
    30361 tgaagaggtc attttaaggc ggtttcagaa ttaagagagt ttttattttc tgagcaagtt
    30421 tatttggtaa aagtttttct attttaaccc ctaacttccc acaaatcaag taatcggtat
    30481 tctgaaatgt tatttttaag aggtaaaaaa tacaaatttt taaaaaaaaa taaacatata
    30541 aattttattt aaattgcttg atagtggtat attaacatat aaattttcaa aggtattctg
    30601 caggatttca ataaaaaaga ttttaaatat tgacagggag ataggattaa attataaaat
    30661 tacttagtaa gtaatataaa aggaggcggg tatgagtaaa actgaaaaag ttaaagaata
    30721 cgcaaaaaaa tgtggagcag accttgtggg gatttcctca ccggagagat ttgaaggtgc
    30781 acctctccag atggaccctc gtcaaataat gcccggagta aaatcaataa ttgttttagg
    30841 attcagagta ttcagaggga catttagagg gattgaagaa gggacatttt tcagtaatta
    30901 ttcatctatg ggatatggag gaataagtaa tattcaaatg ccaatggtgc tctggaattt
    30961 ttgtaaaata atagaagata agggatatga ggcaattcca ttaggacatt tgagtccatg
    31021 gagagcaatt gatgaaaatg gtgaaattaa aaaaggatat agtcgaccag ttgctccagg
    31081 taaacctacg ccggatgtaa tgattcatca taggatagcg gctttttgtg ctggattggg
    31141 agagattgga tatagcaagg tttttctaac acctgaattt ggaccaagac agagattagg
    31201 ggaagttctg actgatatgc ctttagaccc tgacccacta tatgaaggac ctgcaatttg
    31261 tgatagatgt atggaatgtg taaaaggatg tccagcaggt gcaataagta ggataaaaac
    31321 tgtaaaagta gaagttgcag gtaagatgtt agaatgggga gaattagatg tagaaaaatg
    31381 ttcaataggg ttacgaggag gaagaaaagt aaaagaggga gagaaaggag attatctaca
    31441 gggtcgttct gatgtaaaac cggggatgca ttcgccattt tatcataaac cccccaatgt
    31501 ttttattcat ggagaagcga tatgtggtgg tagagggtgt attagagcat gtatgattca
    31561 tttagaaaaa cagggaaagt taaaaaataa atttaaagac ccatttagaa aagaaacacc
    31621 atggagggta aactgggatt aatacagata aattataaag tttctattct aatccgtaaa
    31681 gtaaaaggag tgttttatgg caaatgtaca ggagataaaa attaaatcaa gtgtagatgg
    31741 tactacccaa cctaatataa ttattacttc atcctcatat aatattgaga attttgttcc
    31801 tttacttgtt gctttacata cctggcaggg tgattataag caattagtga aacccataga
    31861 aaaacagata gaaaaaagga actgggttgc acttcttcct aattttcgtg gtcctaattg
    31921 tttagaaaat cctgattgta agaaggcatg tggttctgaa tatgcacaaa gggatattgt
    31981 agatgcgatt aattatgttt tttcctgtta taaaatagat aaacagagag tttatttaat
    32041 tggtgggtct ggtggcggat atatgagttt aatgatggtt gctaaatatc caaatatgtg
    32101 gtcagcagtt agtacatggg catccattag tgatttaaaa aaatggtggg aagaaaataa
    32161 aaattatagt atacatattg aagcatgctt aggtggtcca ccgggtaaaa gttccgaaat
    32221 tgataaagaa tattatgaaa ggtctccaat taattttatt acatctgcaa agaatgtttg
    32281 gcttgatatt caccatggga taaatgatat ggtagttcca tatcatcaca gtgaagatgt
    32341 ttataaaaaa cttatagatg caggcagtaa aaaggcaagg tttacattat ttaaaggagg
    32401 acatactatt aattattttc aggcatgtca gtgggttagt aaaaaaagca atgaaattgt
    32461 tgttacaact tgaaacataa ataattttta aaaattccat gaagattaga attgttaata
    32521 aaaataagca gtaaaatatg agagaatgta tgatgagttt aagtcctaaa ttcccatata
    32581 tacttgttca attgagtatg ttgaaaagaa gcaagtatta atgacataaa gcaggttttc
    32641 aaacgtttgt tttgtagttt gccacaaaac acatcagatt atttatgtta tagacaatta
    32701 cctttttact cgtgatttgc ctgcccatat atcccatatt tcttcatctc ctctattgtc
    32761 ctatctaaac tgcttacatc tactatattc cagggtaaat actgaaaaat aatggcaacc
    32821 ctttcccttt ggccactaca aataaaaatt ttatctcttc ctctatctta tctatatatc
    32881 cccactcgtt tatatccata tttatctgat taggtagtcc tgttgtatct attattatcc
    32941 cttctttggt aatgtctatt ttttcctgta aaaacaagaa gcaaccatct ataaaatttt
    33001 caactactaa aattgtaaaa catatccaca atctcgcccg ttgggtcttt acgcccgagg
    33061 gtatcctgcg gctttttaaa aatataaaat ttttctcttt ttcttcctaa attatattaa
    33121 aacctgttat atccttcccc ccttctcctg cttccttacc tgctactatt tcttctatct
    33181 cttgacgaac tctcttactt ggtgggagac ttttattact attcattgca acccctttct
    33241 tttttattca tatatacagg atggccttta tctttgtcaa atttattt
//
LOCUS       DAOOVW010000016        33004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_891_length_33004_cov_9.931682, whole genome shotgun sequence.
ACCESSION   DAOOVW010000016 DAOOVW010000000
VERSION     DAOOVW010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 33004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33004
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_891_length_33004_cov_9.931682"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..259)
                     /locus_tag="PLW95_03505"
     CDS             complement(125..259)
                     /locus_tag="PLW95_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21731.1"
                     /translation="MFFSPVGYQSSAIKICPPLIIEEAIKEGCSVIEEAIEEIEKENH
                     "
     gene            1239..2483
                     /locus_tag="PLW95_03510"
     CDS             1239..2483
                     /locus_tag="PLW95_03510"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOV21732.1"
                     /translation="MKKMSSVGLFLLLNSVYFFSYFQRVGIPGTIFNELQSTFSISAT
                     TIVGLGSLTFFVYGLMQIFSGVLSDRFGGFKTFLIGAFLLSVSSILFSFSHSVSMLFT
                     TRAIVGFSAGFIYISLIKILTTIYPEKKFPFYLGITLVLGYSGGIFATYPLERLVSYL
                     GWRNSLFLIGIICSICTIICFSVFRISGTPSTKKQTFSLPLLMGVLKNTRSLPLFISS
                     PINFGIYFLFQSTIGKKFLQDFCAFSSSDASLFTFIMMITNTVFTFVSSYTSGLTGKR
                     RSIITSATFITLTALLILFLNLSFLNKKGLFLVSYILLAIGAAAGPVYTTTMKESNAI
                     SIAATSVGLLNTISYLFIGFLGWCTGFILDHFRNASVEKEGIIIYPPSAYRAIFLFCI
                     ILAVCSFLVSLLIKDKHTKIMQ"
     gene            2640..3824
                     /locus_tag="PLW95_03515"
     CDS             2640..3824
                     /locus_tag="PLW95_03515"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOV21733.1"
                     /translation="MGSYNFFLSKKFLSRKKDDHFLWFINFFSIAGIAIGVIALILVI
                     GVMNGFSNDLKKKIIGANPVITIEGRPSIYDYQNIIKKIKENVEEVEGISPFISTQVI
                     YKSDNYMLGGILKGIESDTEKDVTNLEKFIVKGKYSDIENGIILGKELAKELEVVPGD
                     NVWIIVGLIPKQMCFKVVGIFDYGIYSFDVSTGFVSLKNLMDFLNINSVHGIGIKIDN
                     IYNSKETAEKIKKVIAEEYSVSTWIEKNKILFSALALEKKAMAIILSLIIIVASFNIA
                     STLMISVYKKVKDIGILKAIGLTRWEIKKIFFYQGLILGVKGLFSGLVIGLSLSFIVK
                     KYQFIKIPEFVYDLSHLPIEISVMDILWICILVLVIVSFASIYPSTRAAKLNPAEAIR
                     NE"
     gene            3817..4503
                     /locus_tag="PLW95_03520"
     CDS             3817..4503
                     /locus_tag="PLW95_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015790802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV21734.1"
                     /translation="MNKIKSENITKVYSENVVAIENVNIEIEEKEIVVIFGPSGSGKT
                     TLLNIFGLLDKPTKGNLFFNGELLNNIGERQAAEIRNKKFGFIFQFFYLLPELTVVEN
                     VMLPLWIKEKNKGVKKYYKKAEELLEKFGLLKRRDFYPTQLSGGENQRVAICRSLICQ
                     PEVIFADEPTGSIDKFSAEIFFNFIINLNKEKYTTFVIATHNENFLQIASKVVYLKEG
                     KIEKIEKRSR"
     gene            4505..5383
                     /gene="ilvE"
                     /locus_tag="PLW95_03525"
     CDS             4505..5383
                     /gene="ilvE"
                     /locus_tag="PLW95_03525"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HOV21735.1"
                     /translation="MGLTIYLNGKFVSEEEAKVSVFDHGLLYGDGVFEGIRSYNGKIF
                     KLDEHLKRLYNSAKAILLEIPLEFSELKEATIQTVRTNKLKDSYIRIVVTRGVGDLGL
                     DPNKCKIPTLFIIASKIQLYPEVVYEKGIDVVAVATRRNANESINPAIKSLNYLNNIL
                     AKIEANNAGATEGIILNQDGFVSEGTGENIFIVKGDTLSTPPVSVGTLEGITRDIVME
                     LGKNIGLKVKEDNITRYDIYTCEEMFLTGTAAEIVPVVKVDGRIIGSAKPGKITKKIR
                     DEFINLTQVEGVPVYE"
     gene            5376..5627
                     /locus_tag="PLW95_03530"
     CDS             5376..5627
                     /locus_tag="PLW95_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21736.1"
                     /translation="MNSKKDVFVVYLNNKRITKKKYNKDKICPFCGYHYSDFKKIGFL
                     GCPECYNAFYPEIIKFLKKIHRSIEYKGKIPEKYEDRKC"
     gene            5608..6639
                     /locus_tag="PLW95_03535"
     CDS             5608..6639
                     /locus_tag="PLW95_03535"
                     /EC_number="2.7.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP--guanido phosphotransferase"
                     /protein_id="HOV21737.1"
                     /translation="MKIENVNLLSSNIIEEKTRWVFEKISFNNVITSRVRMARNLNNY
                     PFPHNMTFSEAKEVENVVLKKILSYPYDEIFLIRTDQIDKKEKQILIERRIISNDLIE
                     NSVPSSVAILPNERVSIMVNEEDHLRIQSIQPGLNLKEGLKKVNKIDNFLQQEIEYAF
                     SQKIGYLTACPTNIGTGIRGSVLIHLPALSILKITSEIFSAIENIGIMVRGFYGEGSK
                     PLGNIFQITTKETSGKRGKQIIEELEVIVNIILQEEEKAVERLRKMKRIREKINKKIR
                     EIDKYKSINKEKFLYFFSLMYLGKKLDILNIDDEIFHNLFIWILNSSLKFTEKKISIS
                     KNVLKEVKL"
     gene            6665..9172
                     /locus_tag="PLW95_03540"
     CDS             6665..9172
                     /locus_tag="PLW95_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOV21738.1"
                     /translation="MFERFTRRVRTIIDLARKKAVNYGNNEIEPEHIFLAMIEEGQGV
                     GISILHHFGVDLNKVKNEIERYMKIGIVSYVRELPLSQQSKKVLELSFREAQFLGHNY
                     IGTEHLLLGLVADTETIPSKILRRDGISLDRLRRTFEQMLTDGGQLAAENSFGENRYR
                     QQYSTGRKSKTPALDTFGKDLTKEVLEGKLDPVIGREKEILRVIQILCRRKKNNPVLL
                     GDPGVGKTAIVEGLSHMIASGKAPDILKDKRIIMLDLPSMVAGTKYRGEFEQRVKTVL
                     DEIKNSNNIIIFIDELHTLVGAGGAEGAIDASNILKPPLSRGEIQCIGATTLDEYRKY
                     IEKDGALERRFQPVFVSPPTVSETIDILNGLKGKYEAHHNLKITDETIETAVKLSEKY
                     INGRNLPDKAIDLLDEAMSQLRVKLFNPPEEIEKLEKEITEVTKKKEEAINKQNYEKA
                     AIIRDNERELKNKLDYLKENWQKLIPEEKRIVTPESIADVVSQWTGIPVYKLRKEEKD
                     KLLEIESAIHKKVIGQQEAISAISRAIRRSRAGLKDSRKPIGSFLFLGPTGVGKTLLA
                     RVLAEFLFGDEQALIQIDMSEYMEKFSVSRLIGAPPGYVGYEEGGQLTEKVRRRPYSV
                     ILLDEIEKAHPDVFNLFLQVLEDGRLTDSFGRSVDFKNTILIMTSNIGTNVFKNKAII
                     GFGEQEDEISFSSLKEQIMERVKKTFRPEFLNRLDEIVIFHPLEKEHLYSILEIELKE
                     VEERLKENNISIVLTDEAKNFLIEKGTNPEFGARPLRRTISRYLEDPLSEEILKDKFP
                     PDLVVIVYRKGEQLSFRLQKKGEKFEENHNSASVIPT"
     gene            9132..11360
                     /gene="bamA"
                     /locus_tag="PLW95_03545"
     CDS             9132..11360
                     /gene="bamA"
                     /locus_tag="PLW95_03545"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOV21739.1"
                     /translation="MRKIIIPLLLFLLSFSVYSFSQSYIREIKLSGNKNVSEEKIFSL
                     IKTKIGSEFSEEGIRNDIKVISQTGYFKNVGYEYEQKEDGVYLKILLEENPSIREIYF
                     SGNRVFKKKKLLEFLGINKGDVFNEVKLKEGIEQIRQNYIKKGVNLAEISYEIKQVDE
                     KVDIHIKIDEKGGRNCIGEIVFKGNKSFSNKELSKCIKHKRRKMPIFRGTFKQDVLEK
                     DIESIKKFYKKNGFLDVQIEGKIIPVRHSLIKIEFDIKEGEKYFLGDIDFQGNLIFDK
                     NKLFSALTLKNKGEVFNEEKAQENIRNILDIYYNKGYIKASVTPIPTIQDKYINITYY
                     IEPENVYHAEEIKVKGNTKTKDKVIRREVEIYPGDIITAKKIKESWENLRALNYFDEI
                     NIFPEFTEKESANVVVDVKERERTGIFMLGGGYSSVDNVVGVISIQQNNFDITNPPHF
                     IGGGQRISLTTEIGTEAENFTLSFTEPYFLDKPIWFGPDIYRLTHEWDDYTIERTGGD
                     LRIGRRWEKFSFGAKFLSEKNDLSDVDIPSIRYQEGDLTKNSINFSFVYNNFDSKSSP
                     TIGDKLDLEVEYAGGILNGDIDFIKPTIENNYYHPLGRWTFHSRTYAGWLSEDIEDIP
                     IYERFFGGGIGTVRGYDERTLSPKDAQGYYVGGKTLFAQNLEMIYPLYKDMLKGVLFF
                     DIGNVSNKWDFSELKKGVGAGIKVIIPFFNAPIEVYYGYALDEEPGEDKGQLHIGMSF
                     GF"
     gene            11560..12075
                     /locus_tag="PLW95_03550"
     CDS             11560..12075
                     /locus_tag="PLW95_03550"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOV21740.1"
                     /translation="MKKKILFGVVCSIFLFSIVSFADIKIGYVDMQKIFTNYKKAIES
                     EETFNKEVETEQKKINDLQEEIKKMQAEYDAKKDILKPDEKTKKEEELKTKMEEFTKQ
                     WSDVNKKLTDRNNELINQRLEEIKKVISDYGKKNGYSIIMNNINNQIILYGDEKGDLT
                     DKIIEVLNSQK"
     gene            12082..13149
                     /gene="lpxD"
                     /locus_tag="PLW95_03555"
     CDS             12082..13149
                     /gene="lpxD"
                     /locus_tag="PLW95_03555"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOV21741.1"
                     /translation="MNMRVKEIAEIVGGKVIGDENIVITGISGIKEAEPGDITFVATN
                     KYKNLLKTTKASAVLVSPEINNTVSTTLIQVENPSLSFAKIMELVGDEPVKFTAGIHP
                     TAIIGENVKLGRNVSIQPFAVIEDNAEIGDNTVIGAAVYIGHYTKIGKDGLIYPHVII
                     RERVKIGDRVILHPGTVIGGDGFGFATVRGIHHKIPQIGTVEIGDDVEIGSNVTIDRA
                     RFDKTYIGNGVKIDNLVQIAHNVKIGDNTIVVAQVGISGSTEIGKNVIIAGQAGIIGH
                     ITVGDNAIIGGKAGVTKDVPPNTHVTGFPARDKWEDMRFQVFVRKQPELNERIKKIEE
                     KIENLKKILEHLYGEEISKND"
     gene            13121..14446
                     /locus_tag="PLW95_03560"
     CDS             13121..14446
                     /locus_tag="PLW95_03560"
                     /inference="COORDINATES: protein motif:HMM:NF009667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HOV21742.1"
                     /translation="MEKKYQKTIEKDTFLEGIGLHTGEKARVIFKPASVSSGIFFLRK
                     DIENTEPIRADITNLLDTLKYPRRTSIGNEEIQIHTIEHLLSALYALEIDNVFIEIYG
                     PECPGLDGSSKPFVDTIKKAGVKTQDELKDIYKIKDPIYISNNGSHIIALPSEKLKIS
                     YTLSYPSTCLKSQFASYEINPEIYEKEISPARTFCLEEEVENLREIGLGKGSNYENTL
                     VISETGPINNKFRFEDEPVRHKIDDLIGDIALLGYPIQAHIIGIRSGHLLNTKLVRKI
                     KEVVKKEKASAVVSPSISGSVTGEELNIEDIEKIIPHRFPFLLIDKILKIEENKAIGI
                     KNVSINEWFFQGHFPGKPVMPGVLIVEAMAQVGGVLMLNKEENRGKLAYFMSIDKVKF
                     RRAIKPGDQLLIEVEIAKLRSKVGQIQGRAYINGKIAAEALLMFTVVDA"
     gene            14726..15496
                     /gene="lpxA"
                     /locus_tag="PLW95_03565"
     CDS             14726..15496
                     /gene="lpxA"
                     /locus_tag="PLW95_03565"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOV21743.1"
                     /translation="MKKIHPTAIISTEAIIEDGVEIGPYSVIEGNVKIGERCIIGPHV
                     QISGWTEIGKNCKIFKGAVIGSIPQDLKYKGEKTFLKIGDNNIIREFVTINPGTSKGE
                     WTIIGNNNLLMAYSHIAHNCVLGNNIIISNVGTLAGHVKVDDYAIIGGMVGVHQFCKI
                     GKHSIIGGCSKVTKDILPFIMADGHPAIPCGINTVGLKRRNFDKEKIELIESAYKIIF
                     RSGFNIGEAVLKLEGINKCEEIKEIIEFIKASERGIAK"
     gene            15493..16263
                     /locus_tag="PLW95_03570"
     CDS             15493..16263
                     /locus_tag="PLW95_03570"
                     /inference="COORDINATES: protein motif:HMM:NF017989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxI family protein"
                     /protein_id="HOV21744.1"
                     /translation="MKEKIAIFLSDDEFSKYSFKKLKKNFIPIPFSFKKLKFTKTNIL
                     SPYNLSEIFDFLKKAKIKKIILVGKIPATILFDKKFDFLGRQLLTSTSNWTGEEVLKK
                     GISLLKNYGIETISLKEFFKDEIAERKIYCGNLEENEKKDIKTGISFLLDIEKYRCGQ
                     SIVIKNGMLICLEGIEGTDEMIKRAGRYCSDFVFVKIAGKQKDERFDLPVIGPDTVKL
                     IAKTKGKVIAVESGKTIIFDKEKTINYCKKFNITLVGI"
     gene            complement(16304..17554)
                     /locus_tag="PLW95_03575"
     CDS             complement(16304..17554)
                     /locus_tag="PLW95_03575"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOV21745.1"
                     /translation="MEGKINLFQMAQKQLDKSFEKLNLDTNIQSILRVPMKEIHISIP
                     VKMDNGNINIFKGFRVQYNNALGPFKGGIRFHPNETIDTIKALSCWMTWKCAVANLPF
                     GGGKGGVICEPRNMSDRELEKLSRGYIDLLSDEIGPEKDIPAPDVYTNSKIMGWMMDE
                     FSKIKGVNSPGVITGKPICIGGSQGREDATARGAIYCVREASKYLNMDLSNTTVAISG
                     YGNAGSHMAILMKEIFGSKIVAVSDSRGGIYNKDGLDPKGVLEYKYKNNSLMDFPDAE
                     NITNEELLELKVDILCPSSLEGIITEKNANNIKCKVIAELANGPTNPSADEILFERGV
                     TIIPDILCNSGGVIVSYFEWVQNICGYYWPLKKVYQNLDRQITTAFKNVMSMAIKNNI
                     CLRTSAYMVAVKKVVEAMKSRGWV"
     gene            complement(17738..18751)
                     /gene="ruvB"
                     /locus_tag="PLW95_03580"
     CDS             complement(17738..18751)
                     /gene="ruvB"
                     /locus_tag="PLW95_03580"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOV21746.1"
                     /translation="MEKEITSPMAGNEDKQFEQKLRPLTLDEFIGQDKIKENLKVFIS
                     AAKERKEVLDHVLFYGPPGIGKTTLAYIIANEMGVNIKSTSGPVLEKAGDLAGILTNL
                     EFSDVFFVDEIHRLSRNVEEYLYSAMEDFVIDIMIGEGPKAKSVKIPIKPFTFIGATT
                     RMGLITSPLRNRFGILHRLDYYANSEIEKIIKRSAKILNIEITDEGTKEIAKRSRGTP
                     RVANRLLRRVRDFAQVKGKGIIDKEIAVYSLNQMDVDEEGLDEMDKKILEVIITKFNG
                     GPVGIKSISIAVGEEPSTIEEVYESFLVRKGFIKKTQQGRVATYQAYKHLGVDKKTPD
                     DLF"
     gene            complement(18753..19346)
                     /gene="ruvA"
                     /locus_tag="PLW95_03585"
     CDS             complement(18753..19346)
                     /gene="ruvA"
                     /locus_tag="PLW95_03585"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOV21747.1"
                     /translation="MIYYINGKIVEKNPIKVVVEKDGIGYVVNVSLQTSENIGNPGDD
                     VKLYTVLKIADEDINLYGFSTDHEREFFLLLIGVPNIGPKVALRILSGSSVEEIYNAI
                     SNEDPSFFTKIPGVGKKTGERLIVELKQKIEKIELVPSVESIKTDRELFSNAVDALTV
                     LGYKRAEAISSVKKILSQFNNKSLTLEEVIRETLKKL"
     gene            complement(19315..19815)
                     /gene="ruvC"
                     /locus_tag="PLW95_03590"
     CDS             complement(19315..19815)
                     /gene="ruvC"
                     /locus_tag="PLW95_03590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOV21748.1"
                     /translation="MRTLGIDPGIWKVGYGIVEKKGNCFSVISTGFIVPPKNLKYKDK
                     FSHIAKEVEKILENANMDFVAIEEIYIAKNVQIALKIGQVIGIIEAIAMRHNIDFETI
                     PPREVKKCLTGIGSADKGQVKFMVETITGYKNFKNVDESDAVAVAISSFYLKEENDLL
                     YKWKDS"
     gene            complement(19818..20567)
                     /locus_tag="PLW95_03595"
     CDS             complement(19818..20567)
                     /locus_tag="PLW95_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOV21749.1"
                     /translation="MSGHSKWHSIKHKKMATDQKRGKIFTKLIKEIMVAAKAGGPNPE
                     SNARLRLAIEKAKEYNMPNDNIQRAIKKGTGEEGGMNYETVYYEGYGPGGVAIYMEAV
                     TDNKNRTASEIRSLFTRHNGNLGGQGSVAWIFERKGFITVKKDKCSEDKLLEIVLESG
                     AEDMSAEKDVYEITTSPENFEKVKKALEENNIEIESSSFTLIPKNTVKVEGKTAEQLL
                     RLLDELEDNEDVQNVYANFDMPDEILEGISK"
     gene            complement(20564..21100)
                     /locus_tag="PLW95_03600"
     CDS             complement(20564..21100)
                     /locus_tag="PLW95_03600"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOV21750.1"
                     /translation="MMVKVEVEGVVLNLVTHSPIVILKSKKDEILPIVIGIFEAQAIL
                     MVLEKSSFPRPLTHDLIKNIIEILGGKITKVEIYSIKENIYFANLIIEYNGKTETIDC
                     RPSDGIALALRFGADIYADENLLEKAEIIKYYEGQDFVSSNKFNKPIDKKEAEEFRKL
                     IENISAKDFWEKLKGEGK"
     gene            complement(21102..23111)
                     /gene="fusA"
                     /locus_tag="PLW95_03605"
     CDS             complement(21102..23111)
                     /gene="fusA"
                     /locus_tag="PLW95_03605"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOV21751.1"
                     /translation="MGKIYNIGLFGESKSGKTTLGESLLLQAGAIDRMGTTNQGNTVL
                     DFEAEEKERQISISLAVAYFKKNDNIFYIVDTPGYMDFIGEQISGIEGVDIAVINIGS
                     DIGIDVGTEKLWEIIKGKNIPSFIFVNKIDVSDINLNSLWSEIESFFGKKLALITYPI
                     KKGDNFEIVNILQNQVEEDIKGLFQKTMDAIAELDDNVMEKYLEEGKLEKEEISKCLK
                     QGILKAEIMPVLFGSSLKLIGIKELFDFIQNYLPSTDELPPVKGIGPDEKEIERERKA
                     EVPLSGKVIKTNFDPLAGRLSYLRVFSGVLQSNSQIFNSSKNIKERIGQLYRMMGKKQ
                     EPVESVLPGEIVTVTKLQETETFDSFSDPSSPIVFKKPNIPTGAVSYSITPKIKGTED
                     KLGNALNKIVEEDLTINVFRDEETGETLLTGIGDLHIEITTKKFKNKFGVEVEKGIPK
                     IAYKETITKEVNAEGKFKKQTGGRGQYGHCFIKIEPLPRGSGFEFVDKIFGGAIPRQY
                     IPSVEKGIRQAMKKGVLAGYPLVDFRVTLYDGTYHPVDSSDIAFQVAGSMALQKGVQD
                     AGPILLEPIVNVEIRVPQEFVGDVIGTINAKRGKVLDMTIIGKNQVIKGQVPLSEMAN
                     YTNELRSITSGKGSYSISFSHYEPVPSYIAEKIIAERKKEKEKVQ"
     gene            23428..25632
                     /locus_tag="PLW95_03610"
     CDS             23428..25632
                     /locus_tag="PLW95_03610"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOV21752.1"
                     /translation="MNNKKYALVVCGGGLAGVCAAVSSARYGVKTAIIQDRPVFGGNA
                     SSEIRVNIGGAASFNPWARETGIMSEICLKERRINFNYHGSSKTNSNLDLVLYDLLKK
                     ENVDMYLNTSVRKAIMKRSDLIEGVLCIELGSERDFVVYGDYFIDATGDGVVAFSSGA
                     EYRIGREGRDEFRESLAPEESDMGIMGSSLMFLVRDVGKKVKFDPPSWIVKYKKDDIT
                     LEKRYHLSQPGYWWIEIGEPFDIIHDNEEIKHQLLAHLLGIWDHLKNEGEHGFDNFVL
                     EWVGMVPGKRESRRIIGDYILTENDLKERKKFPDAIAYGGWGIDLHTPGGILAKEEPP
                     EATHTEDPEEYWKQVDKTHVYIYQIPYRCLYSKNIKNLLMAGRNISVSHVALGSTRLM
                     ETNALLGQATGIAVYLCKKYNCFPRDITKNYIKQLQQFLLRDGVFIPDVQNEDKNDVF
                     LSSQVKGSSEIPLKIPDENLQLIKLEFPIGQKLPLCGNIKKIIFKIKVLKDTTLNFHL
                     FSSFDLWDFEKGKEIKSVKINVKKNQEIIEIPVNRKLEKGIYWFYFEKNENIYLNEVE
                     DGIPGCCKIIKPGKKWVSKAGINSKNIYFKTIPEIYCFSPENVMSGVSRPEKWTNVWI
                     SENKYPQFIEFEFKNEERLNYIQFIFDAVIEKEYHQIPPFYIPESIPAQYKIYSFKNS
                     KWNLLLEEKNNTCYFKRHFFKEIKTKKIKVEFLKTHNKNLKLWEVRGGMWET"
     gene            25620..26078
                     /gene="smpB"
                     /locus_tag="PLW95_03615"
     CDS             25620..26078
                     /gene="smpB"
                     /locus_tag="PLW95_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOV21753.1"
                     /translation="MGNIIRKIENRKAYHDYEIIEKVESGIVLKGSEVKALREGRGNL
                     KDSYAKVINGEMYLFNFYISPYPNSFEKINPLRKKKLLLHKKQIIKWKQKMEEKGLTI
                     VPLSVYFNTEGFAKVELALAKGKKLYDKRKAIKERYIKREIERKIKEKGI"
     gene            26094..26444
                     /gene="ssrA"
                     /locus_tag="PLW95_03620"
     tmRNA           26094..26444
                     /gene="ssrA"
                     /locus_tag="PLW95_03620"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            27088..27411
                     /locus_tag="PLW95_03625"
     CDS             27088..27411
                     /locus_tag="PLW95_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21754.1"
                     /translation="MIEGKIVKIRFKKYFEEQKLWVFVGKVLKFTENWIMLEGKGIIY
                     LKGQINPVDIDKNKRIILVPRENIFHIRILPDDFDIENIEVITKGVRIFLKVKSGPDT
                     SISEI"
     gene            27500..27571
                     /locus_tag="PLW95_03630"
     tRNA            27500..27571
                     /locus_tag="PLW95_03630"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27532..27534,aa:Ile,seq:cat)
     gene            28257..28332
                     /locus_tag="PLW95_03635"
     tRNA            28257..28332
                     /locus_tag="PLW95_03635"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28293..28295,aa:Pro,seq:tgg)
     gene            complement(28911..29165)
                     /locus_tag="PLW95_03640"
     CDS             complement(28911..29165)
                     /locus_tag="PLW95_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21755.1"
                     /translation="MKKKNNKKYISGVLHCNIIKINNFNVEDRPPQNFSKGETTINPF
                     LCKRKGGDGFIKISPCPSFVKRGKFLLLPLIREGRDGFLL"
     gene            29195..30934
                     /locus_tag="PLW95_03645"
     CDS             29195..30934
                     /locus_tag="PLW95_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21756.1"
                     /translation="MRKKGIALIVVLVIVVLFSAFILIVVLSATSAIRRAHYFDDKTT
                     ALTIAEAGLQQVIYNMNYTNYDSGYYPFGVNSDNPLTPSWDETSETHIDTSNFGPQSS
                     CSVRICDGTGEDTRIVSTGKYRGKQARISVEIRSANPIIRGDKLNDGVGTETQGIPEA
                     FNKHTIYANTIGPLSVPNSVTIRGNICAAFWPSSCDAIGEYTLTDTNVSIDTIPRLAS
                     DPSVTFPPSPADDNYADKYDASGNRVEGVPTGDVSTIINDGVWWDGVDTYYFGTDDGT
                     GTDTNHYVFTPHSVKIEGANAVLTNGVKIGNYFKCDGEVRVETAVFSGDNAVVDAETI
                     TIPTGAEIGEIGPTYCLIVKPSTSSSLSIGNNVTINGDFGISPITTPINLSAGSNFRI
                     NGCLFTNESLNINSPITINASPSTKKAGILLYSNVDKTFDLTIANSIGITTGENQVAG
                     IMVYFEKPLGDDYSGKISNITISGNITLNDTENDKFLLINQSDSGYINISSDINGSIY
                     SAYYGGSTSSITLTNGKIYGSIITNGTVNLNGGSIIYNSEAYKKGKGDVYKGFVGGRR
                     VYVPVPGSWRVEW"
     gene            30952..31158
                     /locus_tag="PLW95_03650"
     CDS             30952..31158
                     /locus_tag="PLW95_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21757.1"
                     /translation="METQKNIVNDVENLKEVIRKTIKETIKEEFFKIRLDLTPEVSDK
                     EMAEIEKKYKEPDKKMVYSEWLDI"
     gene            31160..31417
                     /locus_tag="PLW95_03655"
     CDS             31160..31417
                     /locus_tag="PLW95_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21758.1"
                     /translation="MKVFYSNRAYKFIKEQKIEEKVRQLIRKFILKYTGEDININVKK
                     LKGKWINYHRIKSGTIRIILRVDKDNNCIFVDTVDFRGNIY"
     gene            31500..31982
                     /locus_tag="PLW95_03660"
     CDS             31500..31982
                     /locus_tag="PLW95_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21759.1"
                     /translation="MKKLELYLETSIFGFYTDLKRENITKRESIRGLFEQIKKDYFIA
                     YTSPLTIAELTKIPDKEKNILLNLVKQCKIEVVDTEYEELVNLVNKYKNERIIPEKFE
                     ADLYHVGYATILKVDILVSFNIKHIVKEINARKFNGVNLREGYPIIQFRTPEEVLYEE
                     "
     gene            31972..32148
                     /locus_tag="PLW95_03665"
     CDS             31972..32148
                     /locus_tag="PLW95_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21760.1"
                     /translation="MKSRIEKDFLFILHDIRERENLMRKKKKISSVEWLRENKKLAEE
                     TLGYPIKMLVKSEK"
     gene            32220..32423
                     /locus_tag="PLW95_03670"
     CDS             32220..32423
                     /locus_tag="PLW95_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015224895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOV21761.1"
                     /translation="MRKEFTLEFWIDDGWYVGKLKEVPGIFSQGESLKELKENIKEVY
                     SLMKEEWMPLPSGVQTKETKVEV"
     gene            32438..32602
                     /locus_tag="PLW95_03675"
     CDS             32438..32602
                     /locus_tag="PLW95_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HOV21762.1"
                     /translation="MKELVEDGCYLKRHGKKHDIYENIKNGKKAPVPRHSEIKNTLCE
                     LIRKQLGVEK"
BASE COUNT        11833 a         4637 c         5885 g        10649 t
ORIGIN      
        1 ctgttcttac aaggcgtatt cttatagcca taattaactc cttttatatt tttctgcttt
       61 tttttacaag gtaaattata cacaaaaatt aaaatacttt caataccata atttcctgat
      121 atttctaatg attttccttt tcaatttctt caattgcttc ttctattaca ctacatcctt
      181 cttttattgc ttcttctatt ataagtggag ggcaaatttt tattgctgat gattgataac
      241 caacaggaga aaaaaacaaa agcccttttt catagcatct tttaacaatt tcaaatgctt
      301 tatcagtgtc tggttcttct gtcccatttt tttaatttta ccactactca aaaacaatat
      361 tagaatataa ggacttatcc gaatttattt aagggtttaa attaaaatat tatgagttta
      421 aagtataaca tttttaaaag tttgtcaata attaaatttc tttatataat aacatttttt
      481 aggggaaaag aggtagtgtc aaaaaataga tgaaaaaaaa agatgaaaag gaaaatccat
      541 cccttccttc ccttgtcaag ggaaggagta ttttccccct ttgacaaacc gactaaagta
      601 gcagaggtta gcagggcaga gttggtgtgt catcccccac tttgaaaaag gggggttagg
      661 ggggattttc agatagcaca acgtggaaag agggggattt gaagggggat tttcgtcctt
      721 ctccctgttg gggagaaggc tcgcccgtag ggtcctcgct tttggctacg gattctaaac
      781 tacgcccgag ggtggggatg aggggggatt tgatttctcc ttttataaaa ccgactaaag
      841 ttgcttacct tgtttctcag agttggtgct tagcgttttt cagcaagcac aactgctacc
      901 gactaaggaa gcggggagta gcaaagcaga gttggtgttt catcccccac tttgaaaaag
      961 gggggttagg ggggattttc agatagcaca acggagattg agagggattt tataaagagg
     1021 ggtaaggctc gcccgttggg ttctcacttt tggctacgga tccttcggac tgcgcccaga
     1081 gggggagatt tcaaaaggag aggggttaaa gaaggaagag aagcagaaaa aaaggggaag
     1141 ggatataaag acatataaaa accttaaaag acaaagaaag gcattataaa agctcgtagc
     1201 cattttggca gtaccgaaaa gaataaaaag ggttaaaaat gaaaaaaatg agttctgttg
     1261 gattgtttct tcttcttaat agcgtatatt ttttttcata tttccagaga gtagggatac
     1321 caggtactat attcaatgaa ttacaatcca ctttctctat ttctgctact accattgtgg
     1381 gtttaggttc tcttacattt tttgtttatg ggttaatgca gatttttagt ggtgtgttat
     1441 ctgacagatt tggtggtttt aagacatttc ttataggtgc ttttctttta agtgtcagtt
     1501 ctattttatt ttccttttct cattctgttt ctatgttatt tacaacaaga gcaatagtag
     1561 gtttcagtgc tggttttata tacattagtt tgataaaaat cctaacgaca atttatccag
     1621 aaaagaagtt ccctttttat cttggtataa cattagtact tggttattca ggaggtatct
     1681 ttgccacata tcctcttgaa cgacttgtta gttatttagg atggagaaat tctttattcc
     1741 taattgggat tatctgttca atttgtacta ttatctgttt ttctgtattt agaatatcag
     1801 gtacaccatc tacaaaaaag cagacatttt cattaccact tcttatgggt gttttaaaaa
     1861 atacccgttc tcttccttta tttatttcaa gccctataaa ttttggaatt tattttcttt
     1921 ttcaatccac aattggtaaa aaatttcttc aggatttctg tgctttttct tcatctgatg
     1981 cttcgttatt tacctttatt atgatgataa caaatactgt tttcactttt gtttcaagtt
     2041 atacatccgg acttactgga aaacgtcgct ctattataac atctgccact tttattacat
     2101 taacagccct tttaattctt tttttaaacc tttcttttct gaataaaaaa gggttgtttt
     2161 tagtatcata tattttactt gcaattggag cagcagcagg accagtttat acaacaacaa
     2221 tgaaggagag taatgctata agtattgctg cgacctctgt ggggttactc aataccattt
     2281 cctatctttt tattggtttt cttggctggt gtacaggatt tattcttgac cattttagaa
     2341 atgcttctgt ggaaaaagag gggataatta tatatccacc ttctgcttat agggcaattt
     2401 ttcttttctg tattatactt gcagtttgtt cttttcttgt ctctttattg ataaaagata
     2461 aacatacaaa aattatgcaa tagttcttaa tcacaataaa attatttatt agcatattct
     2521 tatctgtttt ttcacagtga aaaatttgaa aacataaata atgtattttc atatactttt
     2581 ttttacacaa ttgatgaatt tttgtaatgt ggtaagtttt caggtataat tgttgcatta
     2641 tggggtctta taattttttt ctatctaaaa aatttttaag caggaaaaaa gatgaccatt
     2701 ttttatggtt tataaacttt ttttcaattg ctggaattgc aattggtgtt attgctctta
     2761 ttcttgttat tggagtaatg aacggttttt caaatgacct taaaaaaaag ataataggtg
     2821 caaatcctgt aattacaata gaaggtagac catctattta tgattaccaa aatataatta
     2881 aaaaaataaa agaaaatgtt gaagaagttg aaggaatttc cccttttatt tcaactcagg
     2941 taatttataa gtcagacaat tatatgcttg gaggtatttt aaaagggatt gaaagtgata
     3001 cagagaaaga tgttacaaat ttagaaaagt ttattgttaa agggaaatat agtgatattg
     3061 aaaatggaat aattttaggg aaagaattag caaaggaatt agaagttgtg cctggtgata
     3121 atgtctggat tattgtagga cttattccta aacaaatgtg ttttaaggta gtcgggattt
     3181 ttgactatgg gatttatagt tttgatgttt caactggctt tgtctcatta aaaaatttaa
     3241 tggatttttt aaacataaat tcagttcatg gaattggtat aaaaatagat aatatttaca
     3301 attcaaaaga aaccgctgaa aaaataaaaa aagtcatagc agaagaatat tctgtttcta
     3361 catggattga aaaaaataaa attttatttt ctgctcttgc ccttgaaaaa aaagcgatgg
     3421 caataatttt aagtttaatt ataattgttg cttcttttaa tattgcttca acattgatga
     3481 tttctgtgta taaaaaggtt aaagatattg gaatactaaa agcaattggg ctaactcgtt
     3541 gggaaataaa gaaaatattt ttttatcagg gattgatatt gggagttaaa gggctttttt
     3601 cagggcttgt tattggtctt tccctgtctt ttattgttaa aaaatatcaa tttataaaaa
     3661 ttcctgaatt tgtatatgac ctttctcatc ttccaattga gatttcagtt atggatattt
     3721 tatggatatg tattttggtt ctagtaattg tgagttttgc aagtatttat ccttcaacaa
     3781 gagcagcaaa gttaaatcct gctgaggcaa taagaaatga ataaaataaa aagtgaaaat
     3841 ataacaaaag tttatagtga aaatgtagtt gcaattgaaa atgtgaatat agaaatagaa
     3901 gaaaaggaaa tagttgtaat ttttggtcca tctggttctg gtaaaacaac acttttaaat
     3961 atttttggtc ttcttgataa acctacaaaa gggaatttat tttttaatgg agaattatta
     4021 aacaatattg gggaaagaca agcagcagaa ataagaaata aaaaatttgg atttattttt
     4081 cagttttttt atctattacc agaacttaca gttgttgaaa atgtaatgtt gcctttatgg
     4141 ataaaagaaa aaaacaaagg ggtaaaaaaa tattataaaa aggcagagga attacttgaa
     4201 aaatttggtt tattgaaaag gagggatttt tatccaacac aactttcagg aggagaaaat
     4261 caaagagttg caatatgcag aagtttaata tgccagccag aagttatatt tgcagatgaa
     4321 ccaactggaa gtattgataa gttttcagca gaaatttttt tcaatttcat tataaattta
     4381 aataaagaaa aatatacaac ttttgttatt gcaacacata atgagaactt tttacaaatt
     4441 gcttcaaaag ttgtatattt aaaagaaggt aaaatagaaa aaatcgaaaa aaggagtaga
     4501 taaaatggga cttacgatat atttaaatgg gaaatttgtc tcagaagaag aagcaaaagt
     4561 ttctgttttt gaccatggac ttctttatgg agatggtgtt tttgaaggta taagaagtta
     4621 taatggtaaa atttttaagc ttgatgagca tcttaaacga ctttataatt cagcaaaagc
     4681 aatattactg gaaattcctc ttgaattttc agaacttaaa gaagcaacaa tccaaactgt
     4741 gagaacaaat aaattaaaag atagttatat aagaatagtt gttacaagag gagttgggga
     4801 ccttggactt gacccgaata aatgtaaaat ccctactctt tttataattg caagcaaaat
     4861 tcagttatat ccagaagttg tttatgaaaa aggaattgat gtagttgcag ttgcaacaag
     4921 aagaaatgcc aatgaatcaa taaatccagc aataaagtcg ctgaattact taaataatat
     4981 attggcaaaa attgaagcaa ataatgcagg agcaacagaa ggaattattt taaatcagga
     5041 tggatttgtt tcagaaggca ctggtgaaaa catatttatt gttaaaggag atactctttc
     5101 tacgccaccc gtatcagttg gaacattaga aggtataaca agagatattg ttatggaatt
     5161 gggtaaaaat attggtttaa aagttaaaga agataacatt acaagatatg atatttatac
     5221 atgtgaagaa atgtttttaa ctggaactgc tgctgagatt gttcctgttg ttaaagttga
     5281 tggcagaatt attggctctg ctaaaccagg aaaaataaca aaaaaaataa gagatgaatt
     5341 tataaattta actcaagttg aaggtgtgcc tgtttatgaa tagtaaaaaa gatgtctttg
     5401 tagtttattt aaataataaa agaataacaa aaaagaaata taataaggat aaaatttgtc
     5461 ctttttgtgg ttatcattat tctgatttta aaaaaattgg gtttttagga tgtccagaat
     5521 gctataatgc cttttatcct gaaataatta aatttcttaa aaaaattcat agaagtattg
     5581 agtataaagg taaaattcct gaaaagtatg aagatagaaa atgttaattt gctttcatca
     5641 aatattattg aagaaaaaac aagatgggtt tttgaaaaaa tttcttttaa taatgttatt
     5701 acttcacgag taagaatggc aagaaattta aataattatc catttcctca taatatgact
     5761 ttttcagaag caaaagaagt agaaaatgtt gttttgaaga aaattctcag ttatccttat
     5821 gatgaaattt ttttaataag gactgaccag atagataaaa aagaaaaaca aatacttatt
     5881 gaaagaagaa taataagtaa tgatttgata gaaaattctg tcccttcatc tgttgcgatt
     5941 ttaccgaacg aaagagtttc tattatggtt aatgaagaag accatttaag aattcagagt
     6001 attcaaccgg gtcttaattt gaaagaagga cttaaaaaag ttaataaaat agataatttc
     6061 cttcaacagg aaattgaata tgcattttct caaaaaattg gttatctcac tgcctgtcct
     6121 acaaatattg gtacaggaat aagaggttca gttcttatac atttacctgc attatcaatt
     6181 ttaaaaatta cttccgagat tttttcagca atagaaaata taggtataat ggtaaggggt
     6241 ttttatggag aaggttcaaa gccattgggt aatattttcc aaattacgac aaaagaaaca
     6301 tcaggaaaaa gaggaaaaca aattatagaa gaattagaag ttatcgttaa tataatttta
     6361 caggaagaag aaaaagcagt tgaaagattg agaaaaatga aaagaataag agaaaaaata
     6421 aataaaaaaa ttagagaaat tgataaatat aaaagtataa ataaagaaaa attcctttat
     6481 tttttttctc ttatgtattt agggaaaaaa cttgatattc ttaatataga cgatgaaatt
     6541 tttcataact tatttatatg gattttaaat tcttctttaa aatttactga gaaaaaaatt
     6601 tccatttcaa aaaatgtgtt aaaagaggta aaattataaa aagaaaagaa ggaggattta
     6661 aaaaatgttt gaaagattta caagaagggt aagaacaata attgacttag caaggaaaaa
     6721 agcagtaaat tatggaaata acgaaataga accagagcat atttttttag caatgattga
     6781 agaagggcag ggagttggaa tttcaatact tcaccatttt ggagtagacc ttaataaagt
     6841 aaaaaatgaa atagaaagat atatgaaaat cgggattgtc agttatgtta gagaactccc
     6901 tttaagtcaa cagtcaaaaa aagttttaga actatctttt agagaagcac aatttttagg
     6961 acataattat atagggactg aacatctgct tttgggatta gtggcagaca cagaaacaat
     7021 tccctcaaaa attttaagaa gagatggtat aagtttagac cgcctcagac gaacatttga
     7081 acaaatgctt actgatggtg gacaacttgc agcagagaat agttttggag aaaacagata
     7141 tagacaacaa tattcaacag gtagaaaaag taaaacacct gcattggata catttgggaa
     7201 ggatttaaca aaggaggtat tggaagggaa attagacccg gttataggaa gagaaaaaga
     7261 aattttaaga gtaattcaga ttctttgtag aaggaagaaa aataatccag tattacttgg
     7321 agaccctgga gttggtaaga ccgctattgt tgaaggactg tctcatatga ttgcctcagg
     7381 gaaagcccct gacattttaa aagataaaag aataattatg ctggacttac cctcaatggt
     7441 tgcagggaca aaatatagag gagaatttga acaaagagta aaaacagttc ttgatgaaat
     7501 taagaattca aataatataa taatttttat agatgaactt catacacttg ttggagcagg
     7561 tggtgctgaa ggggcaattg acgcatcaaa tattttaaaa ccccctcttt caaggggtga
     7621 aatacaatgt attggagcaa caactcttga tgaatacagg aaatatattg aaaaagatgg
     7681 agcgcttgaa aggagatttc aaccagtttt tgtaagcccc ccaacagtga gtgaaacaat
     7741 agatatactt aatgggttaa aaggaaaata tgaagcacat cataatctta aaattactga
     7801 tgaaacaatt gaaacagcag ttaaattaag tgagaaatat ataaatggaa gaaatttacc
     7861 tgataaagca atagaccttc ttgatgaagc aatgagtcag ttaagagtta aactttttaa
     7921 tccaccagaa gaaatagaaa aattagaaaa agaaataacc gaggtaacaa aaaagaaaga
     7981 agaggcaata aacaaacaga attatgaaaa agcagcaatc ataagagata atgaaagaga
     8041 attaaaaaat aaacttgact atttaaaaga aaactggcaa aaattaattc ctgaagaaaa
     8101 gagaatagtt actccagagt ccatagcaga tgttgtttct caatggacag gaatacctgt
     8161 ttataaatta cgcaaagaag agaaagataa acttcttgaa atagaaagtg ctatccataa
     8221 aaaagttata gggcagcaag aagcaatatc tgctataagc agggcgataa gaaggtcaag
     8281 agcagggctc aaggattcaa gaaaaccaat aggcagtttc ctctttttag gaccaacagg
     8341 tgttggtaaa acacttcttg caagagtact tgcagaattt ctttttgggg atgaacaggc
     8401 acttattcaa attgatatga gcgaatatat ggaaaaattt tcagtaagcc gacttattgg
     8461 tgctcctcct ggatatgttg gatatgagga aggaggacaa ttaacagaaa aagtaagaag
     8521 aagaccttat agtgtaattc tgttggatga aattgaaaaa gcacatcctg atgtttttaa
     8581 tctgttttta caggttttag aagatggtcg tcttactgat agttttggca gaagcgttga
     8641 ttttaaaaat actattttaa ttatgacatc aaatataggg acaaatgttt ttaaaaacaa
     8701 agcaattatt ggttttggtg aacaggaaga tgaaatttca ttttcatctc ttaaagaaca
     8761 gattatggag agagttaaaa agacatttag acctgaattt ttaaatcggc ttgatgaaat
     8821 agtaattttc catccattag aaaaagagca tctttactca attcttgaaa ttgaactaaa
     8881 agaagttgaa gaaagattaa aagaaaataa tatttcaatt gtgttaacag atgaggcaaa
     8941 aaattttctt attgagaaag gaacaaatcc tgaatttgga gcaagacccc taagaagaac
     9001 aatatcaaga tatttagagg acccattatc tgaagaaatt ctaaaagata aatttccacc
     9061 agatttagtt gtaatagtgt acagaaaagg ggaacaactt tctttccgct tacaaaaaaa
     9121 aggagaaaaa tttgaggaaa atcataattc cgcttctgtt attcctactt agtttttctg
     9181 tttattcttt ttctcaatca tatattagag aaataaagtt atctggaaat aaaaatgtca
     9241 gtgaggaaaa gatattttct ttaataaaaa caaaaatagg ttctgaattt agtgaagagg
     9301 gtataagaaa cgatataaaa gtaatatctc aaacaggata ttttaaaaat gtaggttatg
     9361 aatatgagca aaaagaagat ggtgtatatc taaaaatttt attagaagaa aatccttcta
     9421 ttagagaaat atatttttca gggaataggg tttttaagaa gaaaaaactt cttgaatttt
     9481 taggaataaa caaaggagat gtttttaatg aagttaaatt aaaagaagga attgagcaga
     9541 taagacaaaa ttatataaaa aagggtgtta atttagctga aataagttat gaaataaaac
     9601 aagtagatga aaaagttgat atacatataa aaattgatga aaaaggtgga aggaactgta
     9661 ttggtgaaat tgtttttaaa gggaataaaa gtttctcaaa taaagaactc tcaaaatgca
     9721 taaaacataa aagaagaaag atgccaattt tcaggggaac atttaaacaa gatgttcttg
     9781 agaaagatat tgaaagtata aaaaagtttt ataagaagaa tggattttta gatgtccaaa
     9841 ttgaagggaa aattattcca gtcagacact ctcttattaa aattgaattt gatattaaag
     9901 agggagaaaa atatttttta ggagatattg attttcaggg caatttgatt tttgataaaa
     9961 acaaattatt ttctgcccta actttaaaaa ataaaggaga ggtatttaat gaagaaaagg
    10021 cacaggaaaa tataagaaat atcttagata tttattataa taagggatat ataaaagcaa
    10081 gtgttacacc aatacctaca atacaggata aatatataaa tattacttat tatatagaac
    10141 cagaaaatgt ttatcacgca gaagaaataa aagtaaaagg gaatacaaaa acaaaagata
    10201 aagttataag aagagaagta gaaatttacc cgggagatat tataactgct aaaaaaataa
    10261 aggaaagttg ggaaaacctt agagccctca attattttga tgagataaac atatttcctg
    10321 agtttactga aaaagaaagt gcaaatgttg ttgttgatgt aaaagaaaga gaaagaacag
    10381 ggatatttat gcttggtggt ggatatagta gtgtagataa tgttgttggt gtaataagta
    10441 tccagcagaa taattttgat attacaaatc cacctcattt tattggtgga ggtcaaagga
    10501 tttctttaac aacagaaata ggaacagaag cagagaattt tacattaagt tttacagaac
    10561 catattttct tgacaaacca atatggttcg gacctgatat ttatagatta actcatgaat
    10621 gggatgatta tacaattgaa agaacaggag gagatttaag aattggaaga agatgggaaa
    10681 aattttcttt tggagctaaa tttttatcag aaaaaaacga tttatcagat gtagatatcc
    10741 catcaattag atatcaagag ggtgatttaa ctaaaaatag tattaatttt tcttttgttt
    10801 ataataattt tgatagcaag tcatccccaa caattggaga taaacttgat ttagaagttg
    10861 aatatgcagg tggaatttta aacggggata ttgatttcat aaaaccaaca atagaaaata
    10921 attattatca tccattaggg agatggactt ttcatagtag aacatatgct ggatggctca
    10981 gtgaagatat agaggatatt ccaatttatg aaaggttttt tggtggtggt ataggaacag
    11041 ttagaggata tgatgaaagg actttatcac caaaagatgc acagggatat tatgtgggtg
    11101 gaaaaacatt atttgcacaa aatttagaaa tgatatatcc tttatacaaa gatatgttga
    11161 aaggcgtatt gttttttgat ataggtaatg tttcaaataa atgggatttt tctgaattga
    11221 aaaaaggagt tggtgctggt ataaaagtaa ttatcccatt ttttaatgct ccaatagaag
    11281 tttattatgg atatgcgtta gatgaagaac ctggagagga taaagggcaa cttcatattg
    11341 gaatgagttt tggtttttag gtagtgtcaa aaacttagat gaaaaataga tgaaatcgcc
    11401 cctcacccct tcccctcggg cgtagtttag aatccgtagc caaaagcgag gaccctacgg
    11461 gcgagcctct ccccataggg gagagggcga gagtgagggg gaaaatgaac agataacaaa
    11521 aattttggta gaaccgtaaa acaagaaaag gagaaaagaa tgaaaaagaa aattttgttt
    11581 ggtgtagttt gtagtatttt tttgttttct attgtttcat ttgcagatat taaaattgga
    11641 tatgttgata tgcagaaaat atttaccaat tataaaaaag caattgagag tgaggagaca
    11701 tttaataagg aagtagagac agagcagaag aaaataaatg acctacagga agaaattaag
    11761 aaaatgcagg cggaatatga tgcaaagaaa gatattttaa aaccagatga aaaaacaaaa
    11821 aaagaagaag aattaaaaac aaaaatggag gaattcacaa agcagtggag tgatgttaac
    11881 aaaaaactta ctgacaggaa caatgaactt attaatcaga ggttagagga aataaaaaag
    11941 gtaataagtg attatggaaa gaaaaatgga tacagtataa ttatgaataa cataaacaat
    12001 cagataattc tttatggaga tgaaaaaggt gatttaactg ataagataat tgaagtttta
    12061 aattctcaaa aatgaggaaa aatgaatatg cgagtaaaag aaatagcaga aattgtagga
    12121 ggaaaagtta taggtgatga aaatattgtt attacaggta tttcaggcat aaaagaagca
    12181 gaaccaggtg atattacttt tgttgcaact aataaatata aaaacttgct taaaacaaca
    12241 aaggcatctg ctgttctggt ttctcctgaa attaacaata ctgtttcaac aactttaata
    12301 caggtagaaa atccatcttt atcttttgca aaaattatgg aacttgttgg agatgaacca
    12361 gtgaaattta cagcaggtat tcatcccact gcaataattg gagaaaatgt taaattaggt
    12421 agaaatgttt ctattcaacc atttgctgtg attgaagata atgcagaaat tggagataat
    12481 acagttattg gtgctgctgt ttatattgga cattacacaa aaatagggaa ggatggctta
    12541 atttatcccc atgttattat aagagaaagg gtaaaaattg gtgatagagt aattcttcat
    12601 cctgggacag ttattggtgg agacggattt ggttttgcaa ctgttagagg aattcaccat
    12661 aaaataccac agattggtac tgtagaaatt ggggatgatg tagaaattgg ttcaaatgtt
    12721 accatagata gggcaagatt tgataaaaca tacattggta atggtgtaaa aattgataat
    12781 ttagttcaaa ttgctcacaa tgttaagata ggagataata caattgttgt tgctcaggta
    12841 ggaatatcag gaagcacaga aattgggaaa aatgtcataa tagcaggaca ggcaggtatt
    12901 ataggccaca ttacagttgg agataatgcc attattggtg gaaaagcagg agtgacaaaa
    12961 gatgtaccgc ctaataccca tgttactggt tttccagcaa gagacaaatg ggaagatatg
    13021 agattccagg tttttgtaag aaagcaacca gaattaaacg aaagaataaa aaaaattgaa
    13081 gaaaaaatag aaaatttaaa gaaaatttta gagcatcttt atggagaaga aatatcaaaa
    13141 aacgattgaa aaagatacct ttttagaagg aataggactt catacaggag aaaaagcaag
    13201 agttattttt aagcccgctt ctgtatcatc tggtatattt tttttaagaa aagatattga
    13261 aaatacagaa ccaatcagag cagatataac aaacttactt gatactttaa aatatccaag
    13321 aagaacatct ataggaaatg aggaaataca gattcataca attgaacatc ttctttctgc
    13381 tctttatgct cttgaaattg acaatgtttt cattgaaatt tatggtcctg aatgtccagg
    13441 tcttgatggt agttcaaaac catttgttga tacaataaaa aaagcaggtg taaaaacaca
    13501 ggatgaactg aaagatatct ataaaattaa agacccaatt tatatttcaa ataatggttc
    13561 tcatataatt gctcttcctt cggaaaaact taaaatttca tatactctca gttatccatc
    13621 tacttgttta aaatcacaat ttgcttctta tgaaataaat cctgaaattt atgaaaaaga
    13681 gatttctcct gcaagaactt tttgtcttga agaagaagta gaaaatttaa gagaaatagg
    13741 acttggaaaa gggtctaact atgaaaatac tcttgttatt tcagaaacag gaccaataaa
    13801 taataaattt agatttgaag atgaaccagt tagacacaaa atagatgacc ttataggcga
    13861 tattgcttta ttgggttatc caatacaggc acatattata ggaataagaa gtggacattt
    13921 acttaataca aaacttgtcc gaaaaattaa agaagttgtt aaaaaagaaa aagcaagtgc
    13981 tgtggtttct ccctcaattt ctggttctgt tacaggagag gaattaaaca tagaagatat
    14041 agaaaaaatt attccacatc gttttccatt tcttcttatt gataaaatat taaaaattga
    14101 agaaaataaa gcaataggaa taaaaaatgt atctataaat gaatggtttt ttcaaggaca
    14161 tttcccagga aagccagtaa tgccaggtgt tttaattgta gaagcaatgg cacaggtagg
    14221 cggagtttta atgttgaata aggaagaaaa tagaggtaaa ttagcatatt ttatgagtat
    14281 agataaagta aaatttagaa gagcaataaa accaggagac caactactta tagaagttga
    14341 aattgcaaaa ttaagaagta aagtagggca aatacaggga agagcatata ttaatgggaa
    14401 aatagcagca gaagcacttc taatgtttac tgttgtagat gcctgatata agatgaaaaa
    14461 gatacagttg actgcattga ctaagaagta gcaagtagaa agtagtaaga taaaaaaaga
    14521 tacaaataaa aagcgaaatc cccttttata tcccccttta ttaaaggggg aattactcct
    14581 tccttttata aaggaaggat gggatggatt ttacttaaca ctttctgcca tagtatatca
    14641 aaactttcac tggggattaa aatttttctt cttactctct gcttactact cactactgtg
    14701 aataagaatg aggttcaata ataaaatgaa aaagatacat ccaactgcaa taatttctac
    14761 agaggcaata attgaagatg gggttgaaat aggtccctat tcagttatag aagggaatgt
    14821 aaaaataggg gaaagatgta taataggacc acatgttcaa atatctggtt ggacagaaat
    14881 tgggaagaac tgtaaaattt ttaaaggagc agttattgga agcattcccc aggacttaaa
    14941 atataaagga gaaaagacat ttcttaaaat cggtgataat aatataataa gagaatttgt
    15001 gacaataaat ccaggcactt caaaaggtga gtggacaatt attggaaata ataatctcct
    15061 gatggcgtat tctcatatag cacataattg tgttcttggt aataacatta taatatccaa
    15121 tgtaggaaca ttagcaggac atgtaaaagt tgatgattat gcaattatcg gaggaatggt
    15181 tggagttcac cagttttgta aaataggcaa gcactcaata attggtggtt gttccaaagt
    15241 tacaaaggat atattacctt ttataatggc tgatgggcat cctgcaattc cttgtggtat
    15301 aaacacagtt ggattaaaaa gaagaaattt tgataaagaa aaaattgaat tgattgaaag
    15361 tgcctataaa ataattttcc gttctggatt taatattgga gaggcagttt taaaacttga
    15421 aggaataaat aaatgtgaag aaataaaaga aattattgaa ttcataaaag catcagaaag
    15481 aggcatagca aagtgaaaga gaaaattgct atatttttga gtgatgatga gttttctaaa
    15541 tattctttca aaaaattaaa aaaaaatttt attcctatcc cattttcatt taaaaaatta
    15601 aaattcacaa aaacaaatat cctatctcct tataaccttt ctgaaatatt tgacttttta
    15661 aaaaaagcga aaattaaaaa aataatttta gttggtaaaa ttcctgcaac tattctattt
    15721 gataaaaaat ttgattttct tggcagacaa ttattaactt caacatctaa ctggacagga
    15781 gaagaagtat taaaaaaggg gatttccctt ttaaaaaatt atggaattga aacaatatca
    15841 ttaaaagaat tttttaaaga tgaaattgca gaaagaaaaa tttattgtgg aaatttagaa
    15901 gagaatgaga aaaaggacat aaaaacaggt atttcttttc ttttagatat agaaaaatat
    15961 agatgtgggc aaagtattgt tataaaaaat ggtatgctca tatgtcttga aggaattgaa
    16021 ggaactgatg aaatgataaa aagagcagga aggtattgtt ctgattttgt ttttgttaaa
    16081 attgccggga agcaaaaaga tgaaagattt gacctaccag taattggtcc agatacagtg
    16141 aaattaattg caaaaacaaa aggaaaagtt attgcagttg aaagtggcaa aacaataatt
    16201 tttgacaaag aaaaaacaat aaattattgt aagaaattca atataactct ggttggtata
    16261 taatatgtcc gacctccttc tggaggtcgg acattctttg cctttatacc caaccccttg
    16321 atttcattgc ttctaccact ttttttacag caaccatata agcagaagtt cttaaacaga
    16381 tattattttt tattgccata ctcataacat ttttaaaagc agttgtaatc tgtctatcta
    16441 aattctgata aacttttttt agtggccaat aatatccaca tatgttttga acccattcaa
    16501 aataggaaac aataactcct ccgctattac acaaaatatc tggaattata gtaacccctc
    16561 tttcaaaaag aatttcatcc gcagaaggat ttgtaggacc atttgcaagt tctgctatta
    16621 ctttacattt gatattatta gcgttttttt ctgttattat tccttctaat gaagacgggc
    16681 ataaaatatc aactttcaat tccaatagct cttcatttgt aatattttct gcatcaggaa
    16741 aatccattaa agaattgttc ttatatttat attctaatac accttttggg tccaaaccat
    16801 ctttgttata aattcctcct cttgaatcgc tcacagcaac aattttagaa ccaaaaattt
    16861 ctttcattag aatagccatg tgagaaccag catttccata tccactgata gcaacagttg
    16921 tattggataa gtccatattt aaatactttg atgcttctct tacacaataa attgctcctc
    16981 ttgcagttgc atcttctcta ccttgtgaac caccaataca tattggtttc ccggtaatga
    17041 caccaggtga attaacccct ttaatttttg agaattcatc catcatccat cccataattt
    17101 tggaatttgt gtagacatca ggagcaggaa tatctttttc aggtcctatc tcatcagata
    17161 ataagtcaat atatcctcta cttaattttt ctaattccct atctgacata tttcttggtt
    17221 cacatataac tccaccttta cctcccccaa atggaagatt ggcaactgca catttccatg
    17281 tcatccagca agacagtgct tttatagtat caattgtctc atttggatga aaccttattc
    17341 ctcctttaaa tggacctaat gcattattgt attggactct aaaaccttta aagatattta
    17401 tatttccatt atccattttt actggaattg aaatatgaat ttctttcatc ggcactctca
    17461 aaatactttg tatattggta tctaaattta atttttcaaa tgacttatct aattgttttt
    17521 gagccatttg aaaaaggttt attttccctt ccataatatc ctccttattt taaaaataaa
    17581 aataattttt tgtccttcat cattgaaagg aaaataaaaa gaacttcttt gttaggtagg
    17641 cacatccttg tgcttttaaa attatacttt aaatatatat ttctgtcaac cactttaccg
    17701 gaaccaggtt cccggaacca ggttcctgga agagggatta aaagagatca tcaggtgttt
    17761 ttttatcaac tcctaaatgt ttatatgctt ggtatgttgc aactcgtccc tgttgtgttt
    17821 ttttaataaa tccctttcgg acaagaaaac tttcatagac ctcttcaatt gttgatggct
    17881 cttctccaac agcaattgat atacttttta ttccaacagg accaccatta aattttgtta
    17941 ttataacttc aagaattttt ttatccattt catcaagtcc ttcttcatca acatccatct
    18001 ggtttaatga ataaactgca atttctttat caattattcc ttttccttta acctgtgcaa
    18061 aatcccttac tcttcttaaa agacgatttg caactcttgg tgttcctctt gacctttttg
    18121 ctatttcttt agttccttca tctgttattt caatatttaa aatttttgct gaccttttta
    18181 taattttttc tatttctgaa tttgcataat aatcaagacg atgcaatatc ccaaatctgt
    18241 ttcttaaagg agaagttata agtcccattc ttgttgttgc tccaataaat gtaaatggtt
    18301 ttatagggat ttttactgat tttgcttttg gaccttcacc aatcattata tctataacaa
    18361 agtcctccat tgcactataa agatattctt caacatttct tgaaaggcga tgtatttcat
    18421 caacaaaaaa aacatctgaa aattctaaat ttgttagaat tcctgcaagg tctcctgctt
    18481 tttcaagtac aggtcctgat gtacttttta tattaacacc catttcattt gctattatat
    18541 aagcaagtgt tgttttccca attccaggag gcccataaaa aagaacatgg tctaaaacct
    18601 cttttctttc ttttgcagca gaaataaaga cttttaaatt ttcttttatc ttatcctgtc
    18661 ctataaattc atcaagagtt aatggtctta acttctgctc aaattgttta tcttcatttc
    18721 ctgccattgg agatgtaatt tctttttcca tcttatagtt tttttaatgt ctctcttata
    18781 acttcctcta atgttaaact cttgttatta aattgagata atattttttt aactgatgaa
    18841 attgcttctg ctcttttata tcctaaaaca gttaaagcat caactgcatt tgagaaaagt
    18901 tctctatcgg tttttatact ttctactgat ggaacaagtt caattttctc aattttttgt
    18961 ttcaattcaa ctatcaatct ttctcctgtt tttttaccaa ctcctgggat ttttgtaaaa
    19021 aaagaggggt cctcatttga tattgcattg tatatttctt caactgatga gccagataaa
    19081 attcttaaag caacttttgg tcctatattt ggcactccaa ttaaaagaag aaaaaattcc
    19141 ctttcatgat ctgtagaaaa tccatataaa tttatatctt catcagcaat tttcaaaaca
    19201 gtataaagtt taacatcgtc tcctggattt cctatatttt cagatgtttg aagagataca
    19261 tttacaacat atcctattcc atctttttca acaacaactt taattggatt tttttcaact
    19321 atctttccat ttatataata aatcattttc ttcttttaaa taaaaggatg aaattgcaac
    19381 tgcaactgca tcactctcat caacattttt aaaattttta tatccagtta ttgtttcaac
    19441 cataaattta acctgccctt tatcagcgga accaattcct gttaaacatt ttttcacttc
    19501 tcttggaggt attgtctcaa aatcaatatt atgtctcatt gcaattgcct caattattcc
    19561 aattacctgt ccaattttta aagcaatttg aacatttttt gctatgtata tttcctcaat
    19621 tgcaacaaaa tccatatttg cattttctaa aattttttca acttcctttg ctatatgtga
    19681 aaatttatct ttatatttca aattttttgg tgggactata aagccagttg aaataacaga
    19741 aaaacaattc cctttttttt ctacaattcc atagccaact ttccatatac ccgggtcaat
    19801 tcctaatgtt ctcaatttta ttttgaaatt ccttcaagga tttcatctgg catatcaaaa
    19861 ttcgcataaa cattctggac atcttcatta tcttcaagtt catctaatag tcgcaataat
    19921 tgttctgcag tttttccttc aactttaaca gtattttttg gaattagtgt aaaacttgaa
    19981 ctctcaattt caatattatt ttcttcaagt gcctttttta ccttttcaaa attttcagga
    20041 cttgttgtta tttcgtatac atctttttct gccgacatat cctcagcgcc actttccaac
    20101 acaatctcaa gtaatttatc ttctgaacat ttgtcctttt tcacagtaat aaatcccttt
    20161 ctctcaaata tccatgcaac acttccctgt cctcctaaat tcccattatg tcttgtgaat
    20221 aaagacctta tttcagaagc agtcctattt ttgttatcag taactgcttc catataaatt
    20281 gccactcctc ctggaccata accctcataa taaactgtct cataattcat ccctccttct
    20341 tcaccagtcc ctttttttat tgccctttga atattatcat ttggcatgtt atattctttt
    20401 gctttttcaa ttgcaagccg caatcgggca tttgattcag gatttggccc tcctgctttt
    20461 gctgctacca ttatttcttt tatcaattta gtaaaaattt tccctctctt ctggtcagtt
    20521 gccattttct tatgctttat actatgccat ttagaatgtc cactcatttg ccctcccctt
    20581 ttagtttttc ccagaaatct tttgcagata tattttcaat taattttcta aattcttctg
    20641 cttctttttt atcaattggt ttattaaatt tatttgaact aacaaaatcc tgtccttcat
    20701 aatattttat aatttctgcc ttttcaagta aattctcatc agcataaata tcagcaccaa
    20761 atctcaaagc aagtgctatc ccatcacttg gtctgcaatc aattgtttct gtttttccat
    20821 tatactcaat tattaaattg gcaaaataaa tattttcttt tattgaatat atttctactt
    20881 ttgtaatttt ccctccaagt atttcaatga tattttttat aaggtcgtgt gttaagggtc
    20941 tcggaaaaga acttttttca aggaccataa gaattgcctg tgcttcaaaa attcctatta
    21001 caattggcaa aatttcatct ttcttacttt ttaaaatcac aataggtgaa tgtgtaacca
    21061 aattcaaaac aactccctct acttcaactt ttaccatcat attattgaac tttctccttt
    21121 tctttttttc tttctgctat aattttttct gcaatgtaag aaggtactgg ttcataatga
    21181 gaaaaagata ttgaataact ccctttcccg cttgtgatag accttaattc atttgtataa
    21241 tttgccattt cagaaagtgg aacctgtccc tttattacct gatttttccc gatgattgtc
    21301 atatctaata ccttgcctct ttttgcattt attgttccta taacatcgcc aacaaattcc
    21361 tgtgggactc ttatttcaac atttacaatt ggttcaagta aaatagggcc agcgtcctgt
    21421 acaccttttt gtagagccat agaaccagca acctgaaaag caatatccga tgagtcaact
    21481 ggatgatatg ttccatcata aagagtaact ctaaaatcaa ccaatggata accagccaga
    21541 accccttttt tcattgcctg tctgatacct ttttcaacag aaggaatata ttgtcttgga
    21601 attgcacctc caaaaatttt atcaacaaac tcaaaaccac tgcctcttgg gagtggttca
    21661 atttttataa aacaatgtcc atattgtcct cttccacctg tttgcttctt aaattttcct
    21721 tctgcattaa cttcctttgt aatggtctct ttataggcaa ttttgggaat tcctttttca
    21781 acttccactc caaacttatt tttaaatttt tttgttgtaa tttcaatgtg taaatctcca
    21841 ataccggtga gtaatgtttc tcctgtttct tcatctctaa aaacatttat tgttaaatcc
    21901 tcttcaacta ttttattaag agcatttccc aatttatctt ctgtcccttt aatttttggt
    21961 gtaattgagt aagaaacagc cccagttggt atattgggct ttttaaaaac aattggggat
    22021 gaagggtcag aaaaggagtc aaaagtttct gtctcctgta atttggtaac agtaacaatt
    22081 tctcctggta aaacactttc tactggttcc tgcttttttc ccatcattct atataactgc
    22141 ccaattcgtt cttttatatt ttttgaagaa ttaaaaattt gtgaatttga ttgcaataca
    22201 ccagaaaaaa cccttaaata agaaagacga cctgccaatg ggtcaaagtt tgtttttata
    22261 acttttccgc ttaatggaac ttcagctttt ctttctcttt caatttcttt ttcatcagga
    22321 cctatccctt ttacaggggg cagttcatct gttgaaggta agtaattctg tataaaatca
    22381 aataattctt ttataccaat taacttcaaa gaagaaccaa aaaggacagg cataatttct
    22441 gcttttaaaa ttccctgttt taaacatttt gaaatttctt ccttttctaa tttcccttct
    22501 tctaaatatt tttccattac attatcatca agttcagcaa tagcatccat tgttttctga
    22561 aaaagtcctt ttatatcttc ttctacttga ttttgtaaaa tattcactat ctcaaaatta
    22621 tctccctttt ttataggata tgtaattagg gctaactttt ttccaaaaaa tgactctatt
    22681 tctgaccaga gagaatttaa gttaatatca gaaacatcaa ttttatttac aaatataaat
    22741 gaagggatat ttttaccttt tattatttcc cataattttt cagttccaac atcaataccg
    22801 atatcacttc ctatatttat aacagcaata tctacccctt ctatacctga aatttgttct
    22861 cctataaaat ccatataacc aggagtatca acaatataaa aaatattgtc gttttttttg
    22921 aaatatgcca ctgccaaact aatacttatc tgccgctctt tctcttctgc ttcaaaatca
    22981 aggactgtat ttccttgatt ggtagttccc attctatcaa tagcacccgc ttgtaaaagc
    23041 aaactttctc ctaatgttgt ttttcctgat ttgctttctc caaaaagtcc tatgttgtaa
    23101 atttttccca tttgttctcc tctttataaa aggtcatata ttttatatag gcaacaagta
    23161 gaaagaaaat tccttattct ctttaatagc aggtcctgat tatattttag gaacgagtag
    23221 ttcacaggct cccgccggcg taaaattcat aaagcgaccc ttagacaaca ataaaagcaa
    23281 agaaaagaca aaaaaattta aaattttttc tctctactca tctattttac tttttaatta
    23341 aatatattat atcagttttc ataaataaaa gaaagatatt tttatttttt cttctcttgt
    23401 cttataatat tgaaaagggg ataaaaaatg aataataaaa aatatgcact tgttgtatgt
    23461 ggtggaggac ttgcaggtgt ttgtgcagca gtttcaagtg cgagatatgg agtaaaaaca
    23521 gcaataattc aagatagacc tgtctttgga ggaaatgcat cttctgaaat aagggtaaat
    23581 attggaggcg ctgcttcatt taatccatgg gcaagagaaa caggaataat gagtgaaata
    23641 tgtcttaaag aaagaagaat taactttaat tatcacggtt caagtaagac aaattcaaat
    23701 ttagacctcg ttctttatga cctattaaaa aaagaaaatg ttgatatgta tttaaatact
    23761 tctgtaagaa aagcaataat gaaaagaagc gacttaatag aaggtgtttt atgtattgaa
    23821 ttaggcagtg aacgggattt tgttgtttat ggtgattatt ttattgatgc gacaggagat
    23881 ggagttgttg ctttttcatc tggtgctgaa tatcgtattg ggagagaagg aagagatgaa
    23941 tttagggagt cccttgcgcc tgaagaaagt gatatgggaa ttatgggaag ttctttaatg
    24001 tttttagtaa gagatgtggg gaagaaagta aaatttgacc caccttcctg gatagtaaaa
    24061 tacaaaaaag atgatattac attagagaaa agataccatt tatcacagcc aggatattgg
    24121 tggatagaga ttggagagcc gtttgatata atacatgata atgaagagat aaagcatcaa
    24181 ttacttgctc atcttcttgg tatctgggac caccttaaaa atgaaggaga acatggtttt
    24241 gataattttg ttcttgaatg ggttgggatg gtacctggaa aaagagaaag tagaagaatt
    24301 attggtgatt acattttaac tgaaaatgat ttaaaggaaa ggaaaaaatt cccagatgca
    24361 attgcttatg gtggatgggg tatagacctt catactcctg ggggtatttt agcaaaagaa
    24421 gaaccgccag aagcaactca tacagaagac ccggaagaat actggaaaca ggttgataaa
    24481 acacatgttt atatttacca aattccttat aggtgcttat attcaaaaaa cattaaaaat
    24541 ttattgatgg caggaagaaa tataagtgtt tctcatgttg cattagggtc aacacggctt
    24601 atggagacaa atgctttact tggacaagca acaggaattg ctgtatatct atgtaaaaaa
    24661 tataattgtt tcccgagaga tattacaaaa aattacataa aacaacttca acaatttctt
    24721 ttaagagatg gtgttttcat tccagatgtc caaaatgaag ataaaaatga tgtatttctt
    24781 tcttcacaag ttaaaggcag ttcagaaatt ccgcttaaaa ttcctgatga aaatttacaa
    24841 ctaataaaat tggaatttcc aattggacaa aaacttcctt tatgtggaaa tattaaaaaa
    24901 attattttca aaataaaggt attgaaagat acaacattaa actttcatct tttttcatct
    24961 tttgacttat gggattttga aaaaggaaaa gagataaagt cagtaaaaat taatgttaaa
    25021 aaaaatcaag aaataattga aattcctgta aatagaaaac ttgaaaaagg aatatactgg
    25081 ttttattttg aaaaaaatga aaacatatat ctaaatgaag tagaagatgg gattcctggc
    25141 tgctgtaaaa taataaagcc aggtaaaaaa tgggtctcaa aagcaggaat aaattctaaa
    25201 aatatctatt ttaaaactat tcctgaaatt tattgttttt caccagaaaa tgtgatgagt
    25261 ggcgtttcaa gacctgaaaa atggacaaat gtctggattt ctgaaaataa atatccacaa
    25321 tttattgaat ttgaatttaa aaatgaagaa aggttaaatt atattcagtt tatttttgat
    25381 gcagttattg aaaaagaata tcaccagatt ccaccttttt atattcctga gtcaattcct
    25441 gcacaatata aaatttactc ttttaaaaat tcaaaatgga acttattatt agaggaaaaa
    25501 aataatacct gttattttaa gagacatttt ttcaaagaaa taaaaacaaa gaaaataaaa
    25561 gttgaatttt taaagacgca taataagaac ttgaaattgt gggaagtaag aggcgggatg
    25621 tgggaaacat aataagaaag atagaaaaca gaaaagcata tcatgactat gaaataattg
    25681 aaaaagtaga aagtggtatt gttttaaaag ggagcgaagt aaaagcattg agagaaggaa
    25741 gaggtaatct taaagatagt tatgcaaaag taataaatgg agagatgtac ttatttaatt
    25801 tttatatttc tccatatcca aactcatttg aaaaaatcaa tccactaaga aagaaaaaat
    25861 tacttttaca caaaaaacaa ataattaaat ggaaacaaaa aatggaagag aagggcttga
    25921 caatagtccc tctttctgtc tattttaata ctgagggatt tgcgaaagtt gaacttgcat
    25981 tagcaaaagg caaaaaatta tatgataaaa gaaaagcgat aaaagaaaga tacataaaaa
    26041 gagaaataga aagaaaaata aaagagaaag ggatataagt taattgaaaa atggggggcg
    26101 aaatagggtt cgacaggaat aagaggagat agagttgcct gtcgggtttg tcaggaacct
    26161 gtaaaaacct gacaaaaaaa taactgctac agcaaataca cttgctgcgt aattaacgtg
    26221 gcactcactc agaaggggtt gtcctcgcct ctctgaagtg agacggaatg aggacctttc
    26281 aaaaaaggtc tgcccttacc ttttttggga gtattaaaag ggatgcgggt gttgttctct
    26341 gtccgatgga gaacaatact caaagaacaa acatcggaat atgcaggtag atactctatc
    26401 ttttctattt ctggacgcgg gggcagtacc cgccgcctcc accaatttcc ctcaattaat
    26461 ttctcccttt ataaaaccga ctaaggaagc aaagggtagc aaagcagagt tggtgcgtcc
    26521 ttttccctcc ttttgtaaag gagggttagg gtggatttgt atagatagca caacggagat
    26581 taagagggat ttataagagg aaatccagga aaatccatca catccttccc tttataaggg
    26641 aaggagagat gcttccccct ttgaaaaagg gggattgagg gggatttatt tccttctccc
    26701 cgttggggag aaggcgggga tgagggggga ttttaagaaa gaataagata aaaggacctg
    26761 gttgtctccg aaggctcgcc cgttgggcta cgcccagagg gatcctttgg atgtgagaac
    26821 cgggtccttg aaaaaataag gtaagggcat ttttataaat ccatcccgac cttcccttta
    26881 taagggaagg agagatgctt ccccctttga aaaaccgact aaggaagcaa agggtagcaa
    26941 agcagagttg gtgcgtcatc caaaaatact cccctctttt ataaagaggg gcagagggag
    27001 attttaagaa gtggatttgt atagatagca caacggagat taagagggat ttaaaggaga
    27061 gggatttgat agggataaga ggaaaatatg atagagggaa aaattgtaaa aataagattt
    27121 aaaaaatact ttgaagaaca aaaattatgg gtttttgttg gaaaagtttt aaaatttaca
    27181 gaaaattgga ttatgcttga aggaaaaggg ataatatatt taaaaggaca aataaatcca
    27241 gttgatattg ataaaaacaa aagaattatt ttagtcccac gagaaaatat ctttcatata
    27301 agaattttac cagatgattt tgatattgag aatatagaag taataacaaa aggagtgagg
    27361 atttttttaa aggtaaaaag tggtcctgac acaagtataa gcgaaattta atatctgaaa
    27421 tttcaaataa aaaattaaaa aggtataata ctttaaagta tctttttatt tccggaacca
    27481 ggttccagaa taattataag gacccgtagc tcaacggcag agcagcggac tcataatccg
    27541 ttggttgctg gttcgaatcc ggccgggtcc acttccttga gagaaacagc aaccaacaaa
    27601 gggtgcaggg aaaagatttt ccctgcgtat tttgtattgg ggggagaaaa ttaggaaggg
    27661 ggaaacaccc cctcctaaaa agcgaagcgc cggttgcggt tcgaatccgg ccgggtccac
    27721 ttccttgaga gaaacagcaa ccaacaaagg ggttctcccc tttaaaaaag gggagttaga
    27781 ggggatttta agggggaaac accccctcct aaaaagcgaa gcgccggttg cggttcgaat
    27841 ccggccgggt ccacttcctt gagagaaaca gcaaccaaca aaggggttct cccctttata
    27901 cttccccctt tgacaaactg attaaagtag cgggggttag caaagtagag ttggtgcgtc
    27961 atcgtctccc agatagcaca acggggatta tgggggattt agagcagtta ccataaatcc
    28021 atccctacct tcccttagaa agggaaggag taacttctcc ctttataaaa gggagattaa
    28081 gagggatttt catccttctc ccctttgcct atccgccgtg tattgtggcg gacctgtccg
    28141 ccgcgcattt tggcggaagg tcctcggaaa tatgtaggaa ttttaatttc atcaggaaat
    28201 ttttcaaata ttttgtaatt tgcaaagagt aagtataaga taaaagaagt aataaacggg
    28261 gcgtagcgta gtctggctta gcgcgcatgg tttgggacca tgaggtcctc ggttcaaatc
    28321 cgagcgcccc gattagcata cttctttgag aggatttgaa cataggttca aaccgactaa
    28381 agttgcttac cttgtttctt agagttggtg cgtcatcgtc tcccagatag cacaatccga
    28441 gcgccccgac tctttttatt gggacgtgcg ctacgttttt tagaggacgc cccgattttt
    28501 tttcatgaac tccttaccca taagttctcc cttttttaaa atgttaagat agaagaacct
    28561 ggttctctgc gagaaccggg ttcttgaaac aataagataa gggatattac tcccctcttt
    28621 agtaaaccga ctaaagtagc gggggttagc aaagcagagt tggtgcgtca tttcccctct
    28681 tggggttctc ccctttaagt aaaggggagt tagaggggat tttgaggggg atttaataaa
    28741 atccaccccc accctccttt tttaaaggag ggagagtatt ttcccccttt aacaaaggca
    28801 tgtccgacgc gctttttgga ggagggataa agggggattt catccttctc cccgctgggg
    28861 agaaggctcg cccgtagggc tacgcccgag ggtgcaggat gaggagggat ttataataaa
    28921 aatccatccc ggccttccct tattaaggga aggagcaaaa actttcccct ctttacaaaa
    28981 gaggggcaag gggagatttt aataaatcca tccccaccct tccttttaca aaggaaggga
    29041 tttatcgtag tttccccctt tgaaaaattc tgtggaggac ggtcctccac attaaagttg
    29101 ttgattttta ttatattaca atgtaaaacc ccgctaatat atttcttgtt gtttttcttt
    29161 ttcaaatgta gtataataaa agcgggaaat agatatgaga aaaaaaggaa ttgctttaat
    29221 agtagtgtta gtgattgttg ttctcttttc tgctttcata ttgatagttg tcctttctgc
    29281 cacaagtgct ataagaagag cacattattt tgatgataaa acaaccgctc tcacaattgc
    29341 tgaggcaggg ctccaacagg ttatttacaa catgaattac acaaattatg atagcggtta
    29401 ttatcctttc ggagtaaatt ctgataaccc tttaacacct tcttgggatg aaacatctga
    29461 aacacatata gatacttcta attttggtcc tcagtcatcc tgttctgtaa gaatttgtga
    29521 tggaactggt gaagatactc gtattgtttc aacaggtaaa tatagaggaa aacaggcaag
    29581 aatatctgtt gaaataagaa gtgccaatcc aataataaga ggagataaat taaatgatgg
    29641 agttggaact gaaacacagg gaatccctga ggcatttaac aaacacacta tttatgcaaa
    29701 tactataggc ccattatcag ttccaaatag tgttaccata agaggaaata tttgtgcagc
    29761 tttttggccc tcttcctgcg atgctatcgg agaatataca cttactgata caaatgtttc
    29821 tattgataca atacctcgcc ttgcttctga cccctcagta acttttcccc ctagccctgc
    29881 tgatgataat tatgcagata agtatgatgc ttctggaaat agagttgaag gtgtgccaac
    29941 aggggatgtt agtactataa taaatgatgg tgtttggtgg gatggtgtag atacatatta
    30001 ttttgggacg gatgatggta ctgggactga tactaatcac tatgtcttta ctcctcattc
    30061 tgttaaaatt gagggagcaa atgcagtatt aacaaatgga gttaaaatag gtaattattt
    30121 taagtgcgat ggagaagtaa gagttgaaac tgctgtattt tcgggagata atgcagtagt
    30181 tgatgcagaa acaattacta tacctactgg tgcagaaatt ggtgaaatag ggccaactta
    30241 ttgtttaata gttaaaccat ctacttcatc ttctttaagt ataggtaata atgttacaat
    30301 aaatggggat tttggaattt cacctattac tacaccaata aatttatcag caggttcaaa
    30361 ttttagaata aatgggtgtc tatttacaaa tgaaagttta aatataaata gtcctattac
    30421 aatcaatgca agtccatcta ctaaaaaagc aggaattttg ttatatagta atgttgataa
    30481 aacatttgac ttgacaattg ccaattcaat aggtattaca acaggagaaa atcaggttgc
    30541 aggaattatg gtttattttg agaaaccgtt gggagatgat tatagtggga aaatttcaaa
    30601 tatcacaatt tcaggtaata taactttaaa tgatactgaa aatgataagt ttttgcttat
    30661 aaatcaatca gacagtggat atattaatat ttcttcagat ataaatggga gtatttattc
    30721 tgcatattat ggaggttcca cttcatcaat tactctaacg aatggaaaaa tatatggttc
    30781 aataataaca aatgggactg tgaacttaaa tgggggaagt ataatttata attcagaagc
    30841 atataaaaag ggcaaaggag atgtttataa agggtttgtt gggggtaggc gtgtttatgt
    30901 tccagttcca ggtagttgga gagtagagtg gtgattaaat agaggtgaga tatggaaaca
    30961 caaaagaata ttgtaaatga tgtagaaaat ttgaaagaag ttataagaaa aacgattaaa
    31021 gaaacaataa aagaagaatt tttcaaaata aggttggatt taactccgga agtatcagat
    31081 aaggaaatgg cagaaattga gaagaaatac aaagagcccg ataaaaaaat ggtttattct
    31141 gaatggttag atatttgaaa tgaaggtatt ttattctaat agagcatata aatttataaa
    31201 agaacaaaaa atagaggaga aagttaggca actaataaga aaatttattc ttaaatatac
    31261 tggtgaagac ataaatataa atgtcaagaa actgaaaggt aaatggataa attatcatcg
    31321 gataaaaagt gggacgataa gaattatatt aagagttgat aaagataata attgtatttt
    31381 tgttgatact gttgatttta ggggaaatat atattaaatg ggaaaggaga tgtttataaa
    31441 gggtttgttg gaggtaggcg tgtttatgtt ccagttccag gtagttggaa agtagagtgg
    31501 tgaaaaaatt agaattatac ttggaaacat ctatttttgg attttacact gatttaaaaa
    31561 gagaaaatat tactaaaaga gaaagtataa gaggactttt tgaacagata aaaaaggatt
    31621 attttattgc atatacttct cctttgacaa ttgcagaact tacaaaaatt ccagataaag
    31681 agaaaaatat acttttaaat ttggtaaaac aatgtaaaat tgaagttgtg gatactgaat
    31741 atgaagaact tgttaattta gtaaataaat ataaaaatga acgaataata cctgaaaagt
    31801 ttgaagcgga cctttaccat gttgggtatg ctacaatttt aaaagttgat attctggtaa
    31861 gttttaatat aaaacatatt gtgaaagaaa taaatgcaag aaaatttaat ggagtgaatt
    31921 taagggaagg atatccaatt atacaattta ggacaccaga ggaggtgctt tatgaagagt
    31981 agaattgaaa aagatttttt gtttattctc catgacataa gggaaagaga gaatttaatg
    32041 aggaagaaga agaaaatatc ctctgttgaa tggttgagag aaaataaaaa attggcagaa
    32101 gaaaccttag gatatccaat aaaaatgctg gtaaaaagtg aaaaataaaa ttatgagggt
    32161 tcactttcca caagttaaca gttggagagt agagtggtga ttaaagaagg aagaataata
    32221 tgagaaaaga atttacactt gaattctgga ttgatgatgg atggtatgtt ggaaagttaa
    32281 aagaagttcc aggaatattc agtcagggag aatcactaaa agaattgaaa gagaatataa
    32341 aagaggtata ttctttaatg aaagaggaat ggatgccatt accttccggt gtacagacaa
    32401 aagaaacaaa ggttgaagtg tgaaatgaca ggattttatc aaagagttag tggaagatgg
    32461 gtgttatttg aaaaggcatg gaaaaaaaca tgatatatat gaaaacatta aaaatggcaa
    32521 aaaggcacct gttccacgcc attcagaaat taaaaatact ttatgtgaat tgataagaaa
    32581 gcaattagga gttgaaaaat aatattttat aggggggcag ggaaaggaat ctcctctctt
    32641 tgacaaagga taagataaaa catggttgtc tccgaaggct cgcccgttgg gctacgccca
    32701 gaggggtcct ttggatgtga gaaccgggtc cttgaaaaaa taaggtaagg gcatttttat
    32761 aaatccatcc cgaccttccc tttataaggg aaggagagat gcttccccct ttgaaaaacc
    32821 gactaaggaa gcaaagggta gcaaagcaga gttggtgcgt catccaaaaa tactcccctc
    32881 ttttataaag aggggcagag ggagatttta agaagtggat ttgtatagat agcacaacgg
    32941 gacaaggggg attgttctac acaggcagtt taaaatatag catacgaacc agccctacgc
    33001 agaa
//
LOCUS       DAOOVW010000017        32991 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_893_length_32991_cov_7.077787, whole genome shotgun sequence.
ACCESSION   DAOOVW010000017 DAOOVW010000000
VERSION     DAOOVW010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 32991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32991
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_893_length_32991_cov_7.077787"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..1229
                     /gene="trpD"
                     /locus_tag="PLW95_03680"
     CDS             219..1229
                     /gene="trpD"
                     /locus_tag="PLW95_03680"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOV21763.1"
                     /translation="MKQLFEKLLLQKTLTIEESYSVFKKIMENEISPIQASAFLSLLK
                     MKGEQASEIIGAVKLLREKSEKINIRRKKVGDTCGTGGDRSETFNISTAAGIVGFGAG
                     LTIAKHGNRAITSKCGSADVMEKLGFNINIPIEKNKEVLEKFGFSFFFAPFYHPAMKN
                     IAPVRKDLPFRTIFNIIGPLTNPVKTSFQIMGVSEYKLLSLIPPVFKSLNIDGFIFFS
                     EDGIDEISLTGKTYIVEVNKYGIREYEIYPQNFGMKKCSINDLKGGTPDENKEIIIRI
                     LQNKEKGAKKDIVILNSAFLLKASGKVDNIKEGIELSKKIIEEGVAYKKFMELVENLQ
                     KI"
     gene            1226..1849
                     /locus_tag="PLW95_03685"
     CDS             1226..1849
                     /locus_tag="PLW95_03685"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDC27 family protein"
                     /protein_id="HOV21764.1"
                     /translation="MKKIFFLFFVFFLLSSSLVAEVDLYFQAPLDFCSYPDVKEGVID
                     IIYLKIDDLWHKGEYNKIFPLLNLITFISPKEETAWELGGWFLINAIAPSYKGEKREE
                     IEKYGIEFFKKGIKENPESYNLYWEIAWYYYNKGEYEKTLEYLNEAEKFSSDYKIKHL
                     KGHTLIKVGNKDEAIKVWEEIKEKFPEYRNVAERIINELKEDIKNDY"
     gene            1839..2624
                     /gene="trpC"
                     /locus_tag="PLW95_03690"
     CDS             1839..2624
                     /gene="trpC"
                     /locus_tag="PLW95_03690"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOV21765.1"
                     /translation="MIIEKIIEEQIKILDIKKREKPIEELKYFKREKIGLVKNLKNRK
                     IGIIGEIKRASPSAGIINNNIDVCEIAKEYEKLGVAGISVLTCSPYFLGSIDDLKKAR
                     EIVSLPILMKDFVFDEYQIYEGNFYGADVVLLILRVIDDKNFKNLLKISEDLDFEIII
                     EVHNENELKRAFSLVDNWKNKIIGINNRDLDTLEVDLNTTLNLIKFIDKDKIPVISES
                     GIKEKKDILKLKKIGVNNFLIGESILKSDKIEKKLKELMEGGD"
     gene            2627..3970
                     /locus_tag="PLW95_03695"
     CDS             2627..3970
                     /locus_tag="PLW95_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOV21766.1"
                     /translation="MENKVILSEKEMPQAWYNILPDLPKPLPPVLNPATQKPVTPEDL
                     LPIFPMSLIQQEFSPERWIEIPDDIKRGYALYRPTPLRRARNLEKAIGTKARIYYKDE
                     SVSPSGSHKPNTAIAQAYYNKMDGIKRIATETGAGQWGSALALGCKMFGIECIIFMVK
                     ISYQQKPYRRLLSQTWGATMYSSPSTQTEFGRKVLEQQPDCLGSLGIAISEAIEVAVK
                     DEKTKYALGSVLNHVLLHQTIVGLETKKQLEMIGEEPDVLIGCVGGGSNFGGFIFPFV
                     QDKLKGKEIKFIGVEPTACPTLTKGPYRYDFGDTAGMTPLLKMFTLGHDFIPAGIHAG
                     GLRYHGAAPLLSHLVNLGIVEAKALPQTPCFEAGVLFAQTEGFLPAPETNHAIRAAIE
                     EAKNAPEGKVIVYNHSGHGHFDLSAYESYFKGELIDYEYPEEKVKESLKKLPNIE"
     gene            4230..4865
                     /locus_tag="PLW95_03700"
     CDS             4230..4865
                     /locus_tag="PLW95_03700"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOV21767.1"
                     /translation="MTKIKICGFTRRKDIEDAIDLGIKWIGLNFYEKSPRYISEKEAK
                     VLIENLPSEVKKIGVFVNPDEKLLFEYVRKLNLDGAQLHGNEPPSLVKRFKKIFPDKI
                     LIKGIRVKNEGSLNKIKNYKFDYLLIDAFDEKLSGGTGKLINYKYLDRKDIPFARIFL
                     AGGITPENVKDIIKRFSPYGIDVASGVEIAPGIKIKEKMKKLVEEVRNAFA"
     gene            4852..6036
                     /gene="trpB"
                     /locus_tag="PLW95_03705"
     CDS             4852..6036
                     /gene="trpB"
                     /locus_tag="PLW95_03705"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOV21768.1"
                     /translation="MLLPDKNGKYGEFGGKFAPETLMPALELLENDYKKFSNDTAFKK
                     ELSQLLSDYTGRPTPLYFAKNLSKFLGFTVYLKREDLAHTGAHKINNTLGQVLLAKKT
                     GKTRVIAETGAGQHGVATATAASLLNMKCDVYMGKIDIERQKLNVYRMKLLGAEVISV
                     SSGSQTLKDAINEAMRDWISNLQTTHYVIGSIVGPHPYPLIVRDFQSVIGKEAKKQIL
                     QKEGKLPDYLVACVGGGSNSIGLFYPFYNDMDVKFIGVEAGGKGLNTDKHAATLCKGD
                     KGILHGSFTYILQDKNGQIHPTHSVSAGLDYPGVGPEHSFYKTTKRAKYVSVTDEYAL
                     LGFHKLSELEGIIPALEPSHAIGYLIKNKKEFKSSDVIVLCLSGRGDKDVEEVAKIEG
                     IK"
     gene            6033..6812
                     /gene="trpA"
                     /locus_tag="PLW95_03710"
     CDS             6033..6812
                     /gene="trpA"
                     /locus_tag="PLW95_03710"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOV21769.1"
                     /translation="MNKIDEKFAELKIKEQKALIGYFTAGYPDFETSKQVILRSVRAG
                     IDILEIGVPFSDPLADGPIIQYTSNIALQNGINMDKCFSLCEEIKDCVSIPYLLMTYY
                     NPVYKYGIEKFAVKAKKCGVSGVIVPDLPFEESELLKKQLKKNNIFLINFLTPFTQFE
                     RGKKIIEQAEGFIYFITIAGITGEKKEFDYNAFKVIEKYKKIKDIPFCAGFGISTVSQ
                     IKKIKKYVDGIIVGSHFLKQLIYGKIENVEKSVSKFKEVLL"
     gene            6809..7534
                     /locus_tag="PLW95_03715"
     CDS             6809..7534
                     /locus_tag="PLW95_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21770.1"
                     /translation="MKILKKIIALVIVVIILGAGFIYWQKYTEKQRIKLAEEEKRKQE
                     EIEREKQRQFLEQKKQEYYSLIDEMRKYFRQGDYKKVKEIAENSFALAKDFNFDTTEI
                     NKILHQIEVINYQRKLEQLDKDNKDIFNYSYVRNELLKIPSLEELTSLKNKIYQKTFQ
                     NEYLVLLELAEKHAIDGKQGKNSKINYFLSKDYLYKAQNLRKTKNLKQDIKREEKIAE
                     IQKDVFFAFNQFKENTIPSSLYQ"
     gene            7581..9197
                     /locus_tag="PLW95_03720"
     CDS             7581..9197
                     /locus_tag="PLW95_03720"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOV21771.1"
                     /translation="MKKRYRKSKEIIAKNMGTSVIKEQLLSSDIYKSRKFSNIGNVIV
                     YIGDVIKCLRLLPDGIVDCVITSPPYWKQRDYKDPHQIGQESTYQEYVKNLVGVFTEM
                     KRVLKPTGTFFLNVGYKYQNKELLLIPELLAIELQKNGWTLLNKIIWNKPNAMPSSFD
                     SRFSNVYEPVFLFVKNESKYKYYLSIDTLRLPVNNFVNNKNPEDILGFEVENSLLKDK
                     KIKGYISKVFKNSRGDILAQVNWDDRKNTIEIVNDFNKESQISIGLICEDCGRTMKHE
                     IDINSHLNCKGFPKPILPPVANFNNLIELSDSSLFSSVSPQFFQNSRRVYNGKFKLSP
                     DNRGASPGARKSLFGEYFVLQRRYEIFQPIIADYLAFWKRKKNITTKEIDKLLGYKDT
                     AGHWFRKDTGSWGRGGSIPLPDDWFKLKEILEFDNIYDRWITETHLVLQTVRAHPKGK
                     NPGDVWNIKLQPFSGNHFAIFPEELVKRCILSGCPEQGVVLDPFAGSGTTGKVAQELG
                     RDGILIELVPKYLDIIKKRCKNIKEVIYVK"
     gene            9187..9942
                     /locus_tag="PLW95_03725"
     CDS             9187..9942
                     /locus_tag="PLW95_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21772.1"
                     /translation="MLNDIELEKKEKQYFDLILLHLKQDLNSLMDGLNSRIKILNNWY
                     DNFIKTARSGYKSSDLDTGAERIFHHFFASILKFPNSSPVGSDLMFELPDSFMHIEIK
                     TALIDNPSDYKGKINIAINQTSYRVNNVFLPNLPQYYKSSSFNEEKPCLTYIIQIVHK
                     HAKPDIKAIKLVCIPNGQLYTHYGKSIFKSGKGGYTKAKDFRYNYSAEPYFKLLSKKY
                     REKIFRIELVYLAKDIKRKDITNLSDVPVHFQF"
     gene            10113..10307
                     /locus_tag="PLW95_03730"
     CDS             10113..10307
                     /locus_tag="PLW95_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21773.1"
                     /translation="MENKELENIIKNRKQWVNSSIENNFDFDSILSGLYSDPSNFIYE
                     LLQNAEDEGATEIRFELYED"
     gene            10481..12172
                     /locus_tag="PLW95_03735"
     CDS             10481..12172
                     /locus_tag="PLW95_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015736857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOV21774.1"
                     /translation="MERTEFPRPDFKRDEYKILNGIWDFEIDKSNTGIERELFLKDKK
                     FSEKIVIPFPPESKLSKINEKDFMVSVWYKKEIEIPENWKNKRIFLNFGAVDFKASVW
                     INEHFLGTHIGGYTPFSFEITNYLKRGKNYIVLNAYDDNRLNLQPAGKQSLKYNSYGC
                     SYTRTTGIWQTVYLVATGKNYIEKIKISPDIENEKVNFFIDTTGGKFLEFEIYFSNEK
                     IGFGKFKVDKNGIYSVPINKIIFWDYKNPALYDVKIKLFEDNKTLVDEIYTYFGIRTI
                     KIAGNRIYLNGEKLFMKMVLDQGYYPDGIYTAKNQEEFKKDILISKNLGFNGARLHQK
                     IFDPIYLYWADKLGYLVWEEYPNWGIKNRISEETFHILLYEWISSIERDINHPSIIGW
                     CPLNETTIFQNSDLVKTLYRITKKFDITRPVIDTSGYIHVETDIYDCHNYKQNPEEFK
                     NDFEKFKTQDTIWKNFPEYDAEYKGQPYWISEYGGIWWSEEESGWGYGDRPKNKKEFL
                     ERYNGLTKVLLNHPKISGFCYTQLYDVEQEKNGLYTYEREVKFNPEIIRRINSGL"
     gene            12339..13625
                     /locus_tag="PLW95_03740"
     CDS             12339..13625
                     /locus_tag="PLW95_03740"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOV21775.1"
                     /translation="MNKDEIKIGLVGCGTVGSAFLKEIIKKKGEIKKKTGLNITLSKI
                     YDKNKKKKSLLPEKFVSSPEDIINDPEIDIFVELIGGISPSYEYVVKAIENKKSVVTA
                     NKALISEKGKELFEKSKQANVYIGFEGSVGGAIPVINILKESFIGNKIMTVMGILNGT
                     TNYILTKMYHEGIDYEQALKDAQKNGYAEVNPSLDIKGIDTAHKLAILTTLSFNKWID
                     WKKIKIEGIDKIEIEDIKFADEFGYRIKLLGISKINNSKKLELRVHPVLISKGHLLSL
                     VDGVYNAVYFEGDMIGKSLLYGEGAGSYAAASSVISDVIGISKKIVNNNKNADILWTN
                     REIEIQPFQELQSRYYFRFTAIDKPGVLSKISKVLGENNISISSVIQKEENPQKAVPI
                     VMLTHKAIENDVEKAINGINKLTIIKKPTVVIRVEE"
     gene            13701..15068
                     /gene="thrC"
                     /locus_tag="PLW95_03745"
     CDS             13701..15068
                     /gene="thrC"
                     /locus_tag="PLW95_03745"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOV21776.1"
                     /translation="MEKILYRSTNGKAPIVSFKEALLNGQAPDYGLYVPTFIPKIRID
                     EIKCLKQKPYFEVAFHILYKFLKGEIGEKELFEMCKNVYDFDVPIIEIEKGKLYILRL
                     DKGPTCSFKDFAAKMMASLMEYFAKEENKKLTVLVATSGDTGGAVANSFYKKENIKVV
                     VLFPVEEVSERQRKQMTTLGENILPIGIKGKFDDCQNFVKRAFNDISLSSLNLTSANS
                     INIGRLLPQIVYYFYAYLKFEKEIVFSVPSGNFGNLMGGVIGKKMGLPVKKFLVAVNE
                     NNEFPEFLRTGEYMPVIPSKKCSSNAMNVGHPSNFARLIDIYGGWIMDERDKDGKVIK
                     YGVMKRKPDMEKMRNDFISFSISNEEVDETIKNYYQKYKIVIEPHTAVGIKSYEKSQI
                     SDLVIVLQTADPAKFPEKIKQLLNIEPKIPEALAKILTKKEDSFDVLENNYDVFKKYL
                     LDKIK"
     gene            15065..16264
                     /locus_tag="PLW95_03750"
     CDS             15065..16264
                     /locus_tag="PLW95_03750"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HOV21777.1"
                     /translation="MKYLILIMDGAADYPIEKLNGKTPLQVAKKINIDKLAEKSRCGL
                     LKTIPENFSSGSVVANLSILGYDPEKYFKGRGVLEAAAMGIKLNKNDIAFRCNIINVS
                     ENKIVSHSAGHFDADEGKQLIDSLNEKLGRENIEFYSGVDYRHVLVMREKYSEKVRCY
                     PPHDFLGENFYSLLPTETEKEGEETSEILREMIEKSKEILEDHPVNLKREKAGKMKGN
                     LMWPWSPGKKPDMETFQERFNIKGAVISGVDLIKGIGIYCGFEVINVEGATGDYRTNY
                     EGKAQKSIESLKEYDFVYVHVEAPDEASHEGNIELKIKCIEDFDKRLVGNILRNIDIS
                     DISIAILPDHYTSVITGKHTGEPVPFLIYKPDQLPDEVKKFDEISCKNGFYKILEGTS
                     FIYKLIS"
     gene            16654..17295
                     /locus_tag="PLW95_03755"
     CDS             16654..17295
                     /locus_tag="PLW95_03755"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOV21778.1"
                     /translation="MYIIIVGCGKTGKYLAEILQADHNVVVIDKEKEAIDNLGEYFNG
                     ITILGDGMDIDVLKDAGIEKADALAVTTSNDNTNVLIAQIGKKIFNLTKVVARVSAPE
                     KAELYRTLGVETVNSTAIFASLIRDKIIEEKFSAYILESSKLTTIEILTNSKLAGKKV
                     NEINIPGEFRIITIVREQEPIIPDDDTEIQKNDTIVGLIKISSLKKLKRELDL"
     gene            17297..17962
                     /locus_tag="PLW95_03760"
     CDS             17297..17962
                     /locus_tag="PLW95_03760"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOV21779.1"
                     /translation="MNILIVGGGNIGYYLAERLSGSHYVVLIEKKLTVGEEIAGKTNV
                     MVILGDGCDPQVLKKAGIKKADVVAAVTGNDIDNIIICQLAKDIYNVERTVAIVNNPK
                     NQIVFTKLGIDVAIDSTAIIAKIIEDEIGWEDFVNLFAFKKGNLSLLRIDLPETSPII
                     NMPIKELNLPEDSVIVAVIRNNKILVPTQDFILREKDEIITITKAGNESDLFKNLIGE
                     IEE"
     gene            18140..19639
                     /locus_tag="PLW95_03765"
     CDS             18140..19639
                     /locus_tag="PLW95_03765"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOV21780.1"
                     /translation="MIPQFSRDDFKVVIYYIGNLIVYLGIMMLLPLFVGIINNEINPS
                     LDFIISSLLCLSIGNFIMYKFPYNPDINWRQGMSIVALSWIVCMFLGAIPLFLSGHYI
                     SYLDACFESMSAFATTGLVLVNDLDHMAYAYNFWRHFMCFIGGQGIILVGLTFSVRGG
                     AGMKVYVGEGREEKIFPNIVQTARFIWAVSIIYLFLGTLVLFFICLFHNITPGRALFH
                     SICIFMAGWDTSGFAVQSQNILYYHSAAINLVTTLICVLGAFNFAVHYTVWTGKIKEL
                     VKNFELKLFFGSIIVLSSIVCLGFLGHFSFPNFFSFFRLTFYHLVSAHTGVGYTNSTI
                     YHFLNYWPSFSLIGLIIAMGLGGCSCSTAGGIKIIRIGIFFKGIKKEIKRLLFPDSAI
                     LVEKFHNIRDVFIQDNQVKYATNIIILYILFYFIGAMVGTFYDYPFLYSLFESVSACA
                     NVGLSVGITTPSMPTLLKITYILEMWFGRLEFISIFVVIGFIFSLRSPE"
     gene            19636..20118
                     /locus_tag="PLW95_03770"
     CDS             19636..20118
                     /locus_tag="PLW95_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21781.1"
                     /translation="MKKIFNFVIFLLIFCFSAFAQEEITGKDLFEKMKQLDNKEVVIV
                     GECIGDIIKGDKNTFIVNIRTDDNYFIGTVLSKEDAEKINHLGKYEEKGDIIKIIGIF
                     HSNCPVHSGEVDIHCANMEILKEGEVYAEKIDMKRFIVGLVLLFIMLFFTKLSFTKSV
                     "
     gene            complement(20142..22892)
                     /locus_tag="PLW95_03775"
     CDS             complement(20142..22892)
                     /locus_tag="PLW95_03775"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046353 - aminoglycoside
                     3-N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HOV21782.1"
                     /translation="MENLNKRIKKEFDLSRWQNLALKIFNIESRFCYSDFEKSAKLCW
                     QELKDAGAKDVELIELKADGGTTYGDFIMPQAWDYEYGFLYIKEPDKFNGKILADTKH
                     HPFHIANRCGDIEETILDVVDIRNIKKYKDLSKNFVFCGNIPPRECRNEIEKTGALGI
                     ISSYSGAPEKRDGVYWINGWAKNSGWYQTKEDRKMVCFSIKPEDGEFLQKLLDKENVK
                     VCASVKSKRYNGKIYSITGLIPGKTKKEIAFLAHLYEPMITDNATGVAGLIEICRVIN
                     ELIKSKKFIPDVGIRFLFSMERYGMAQFFEKKHNVIYAFNVDGITPDIVKSGRMRITL
                     YGSHFTNPFFGDYLFEKLLEKTFPSNLPWKKERAMFEDDTFISDKSTNIPTMYFISHP
                     GKFHHNSADPEIVNWNLGKEILCSLATYVCTLFSNQKRKKYLNVVNTSVKEEFYKYIS
                     YLNLMIAENPEKFTQQGIKERINYISRYLKDKYLSIKIFGDKPEEKILKEIDKIVRKT
                     ITDITKKFDKEISKKEVLLSREDKKSENIVITWRKPVFIFSLAEIPHKERISPPEEFY
                     SVINRVDGKKDMYQIFKEISSEREFYGMNEISEAEKKSLIKYIQYLSNYNYLKFYYKT
                     IVTKQDIKSGLEKLGIKKGDKVIVHSSLSSLGYVEGGAKAVCEALMELIGKEGVLMMP
                     SFNHGEIFRNDPDAYFSPLETPTTNGAVPEAFWKIKDVYRSLNPTHSFAVWGKNAKDF
                     VKNHHKYLTMGKGSPLYLLEKEGGKIILIDALSANTFHHVVEQTNNVPCLGKRTEQYP
                     VKLPSGEIVHIRTWGWRGRACEITDKGAYWDYIRKNKLLKEGKIGGAQVFVLNMKVCR
                     QVIEKFLKGEIKGFSGCRKCKIKPRIVSQTVESDWDNEKETVKSDTTAFVGDYEPVSY
                     CK"
     gene            complement(23367..25769)
                     /locus_tag="PLW95_03780"
     CDS             complement(23367..25769)
                     /locus_tag="PLW95_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21783.1"
                     /translation="MKQKSFFLFLFLFLFSTFASSATEVKSIPSLEEELQKIKEAGIP
                     TTIEELNLPKIPDEENGAVVYNEVFVLMNSLKEKYKEEWKYFPYAGIVDWEDVPEEEK
                     KKVVDLILHNPEFGKMYQLLEKASQMKCQFLMPDKLTDEPSLLFLNILANLRGCTRML
                     CAKAKLEAENKDINKSLNTILVALKLGKSLSNEPLLITQLVRLAIDTFVLSQLEEIPD
                     SKEVNISLYQTLIQNMEEERKCKIISESLKREMILFGLPKMRQYQQSVKEKLPDLEQK
                     SKEMSEEEKEYAKNLFGTDDPEKIQKFAEKYWDEEISVYIENMIKIISFAEKPYWEVK
                     KKIEDLNTEIQKIPEKRAVLAKQTCLAVSRAYLQEANLDAYLGTAEIGLANRIYRQQH
                     GKFADSLNQLTPEILSSLPLDPFSGKDYIYKKKDKGFIVYSVGENEKDDGGVPAKKMN
                     WKGDYDIVWEDKGAEIPISAYAPLFSEIGENKISRGDISRKSDNASPEIIKFEQIEIK
                     VITELPEIFLIKTPGTPETFPQVDVEKLYANLYSLKQQGILDIIQVEVFADEGKKCVL
                     DMRKDIPGTTEKEGMLFEIIPEIIKDNKIRCQIDISTTKLVELKKIKNEGKGEEIYPI
                     MSNREQSTCVDITNEKPAIIGGLIDSPEMISPKNTLKTLTMIKATRYVKPKRSIEFRL
                     VKNTPETGFTQTNIPYSDKKIYVADTSEIDKSDIRGVRACLTNQELEQIKIVFTDEGR
                     KKFANLTESLIGKKLAILVDGQLVSVPVIREKITGGIGTIPMEKEIIEKILDISTEPD
                     EN"
     gene            complement(25796..26659)
                     /locus_tag="PLW95_03785"
     CDS             complement(25796..26659)
                     /locus_tag="PLW95_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21784.1"
                     /translation="MDIITIFCIFIPLILLTLEGWWFWYWFSFGDITHGQLYIIEIIV
                     GTLIFLTIFEVVKGLYFVGFIAIPIPLIIWFLMKQYEDKTNKLNALLQEKEEINNLLN
                     IIENAKEPALLYKALVELGDLYIKKEKFEKGIDCYKRANEIAEKSHIPGLLGLSAKIK
                     LAEKEIKIKKGEIWVCNECSYDNPGNTDTCKICGNKKDVGKSIKSDIVLHKQEIKKEA
                     IPIIFVGITIIIGLYLLFFLVALFGFLYSKHFPLWIYISLIIVISTLVIFLFFKFIVA
                     IRNKIISKFLK"
     gene            complement(26684..27592)
                     /locus_tag="PLW95_03790"
     CDS             complement(26684..27592)
                     /locus_tag="PLW95_03790"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV21785.1"
                     /translation="MEEYVIETQKLTKRYIKHKEAVKSLDLLVPKGSVYVFLGRNGAG
                     KTTTIRMLMGLLQKTSGKSKVLRMDPEKEPVQIKKRVGYVADNQKMYDWMTIEETINF
                     CRPFYSTWNNNLTEDLLNRFELPKKIKVKELSRGMNGKLALLLALSHQPELLILDDPT
                     SGLDPVVRREFLQGIIETIHLETRTVFFSTHIITEAEQIADYVGIIEEGKLLLSCPLE
                     TLKNSVKEIHLIFEDKIPEEIFFNDKNDKLLKKKVIGHELVMIIKNFTPEILKKLEKF
                     GPKSMEVIDLTLEDIFVSLVGKEKSV"
     gene            complement(27859..29880)
                     /locus_tag="PLW95_03795"
     CDS             complement(27859..29880)
                     /locus_tag="PLW95_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21786.1"
                     /translation="MKTLIWKEWREQSPFFFLSIGIIILCRIIPVIIPKFFEYRIDYE
                     FYILSITTILPILFSLFLGSISFTNEFYRNTKEFLLSQPVSTVRIFWTKYFFGLFLLI
                     ILTLLTNIILFSPFPNFIKAGTILTAPQQQFLIKIYLLLCFIIIYSSSCFSSLLLKNT
                     LPAIICTPFLLLFGLLLIIPLVVFLFLISPTKFIFIFFIFSVSIAVFLIFSLVIWQKC
                     LVKEVGCGKKIFITAIIILIVSFGCHSIVNITISRDFNRTIQQAKAEGIKLTPEEVIP
                     PPVPDEKNAAIVYQETFTLVDKLKEKYKTDWYYMPYEGMKKEEELTTQEKNNIKKIMK
                     DPDFIKFYTLIDKAVNMPSCRFDIKYREGPAMLLPHLSKMRQCSRLVAARTYILAENK
                     KYNDALNSVKTGLKIGESLTDEPIIISQLVRIAIDVIAKNSLNYLLNKEDISFSKDNY
                     YDLISIIDKKNRKITRSLNGELVLFGRYVFEKDIPFGYSLRNILNRQNTYLPGIYGSL
                     VGSYLTQPILKNDYAFYIQYFLELIDYSQKPYYLVKNKLDELDRKFFTYNTFSMSKHI
                     LSLIFLPSITRSIEQQSSYNACLDTLKLSLALKIYKEKYGYYPETLSSLVPDIIPEIP
                     IDPFTGNDYIYRKEGRGFIVYSVGSNEKDDGGISDRKQNSDDISFKVLR"
     gene            complement(29877..30263)
                     /locus_tag="PLW95_03800"
     CDS             complement(29877..30263)
                     /locus_tag="PLW95_03800"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOV21787.1"
                     /translation="MYIKIDSNSGVAPYMQIIKKVKYLVACGALKAGDQLPPIRDLAM
                     QLRINPNTVARAYRELRYEKIITSKWGGGTFISDDVKQIAESEKKKIVDEIFDQAIHQ
                     ATILGFTEEEIIQLFTNQLKTRRNKK"
     gene            complement(30356..31417)
                     /locus_tag="PLW95_03805"
     CDS             complement(30356..31417)
                     /locus_tag="PLW95_03805"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOV21788.1"
                     /translation="MRKKIYFVDKIIKEGIDKKIIPGAVLLVGNKNDIIYWKAYGNRE
                     ITPEKIKMGKNTIFDLASITKPVATATSIMILVEESKISVNDKVIKYIPEFGKNGKKE
                     ITIKMLLTHYSGLSNQKFHNTPTKILERICEETPIYKPDSEFNYSCLGYIILGKIVEI
                     VSGKSLDVFTKEKIFEPLGMKDTMFNPPEKLWVRCAPTIYRDGKLLKGKVNDMQAYVM
                     GGVAGNAGLFSTACDLSIFAQFILNEGIYNEVKVLNPEIVKTMISKASPLNKNEYGLG
                     WNISAPNSFVKGSIPSGYSFGHTGWTGCSLWFYPEIDLFIIFLTNQNHPEEKPNGSFI
                     AKQLNALVNDAILLNFVNC"
     gene            complement(31465..32748)
                     /gene="miaB"
                     /locus_tag="PLW95_03810"
     CDS             complement(31465..32748)
                     /gene="miaB"
                     /locus_tag="PLW95_03810"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOV21789.1"
                     /translation="MEKKYYIKTYGCQMNFYDSEKLSSLLDKNGFEKVNKMEEASFVF
                     VNTCSVRKHAEDRAFGFLNSIKSKNKIFCLIGCTPNLYNEEIFKKIPFVDVISGPNTY
                     NQLVEILPEIEKGEKVILTGESEKPFIYEKSKQKNKISSFITISKGCENFCSYCVVPF
                     TRGKLVHKPFEKIIEEIKNNVENGSKEIILIGQNVNEYKYNGHSFVDLLYNVSEITGI
                     ERIGFLTSHPKDIPEELLFSFVKIPNLYKHLHFPLQSGSNKILEKMNRKYTIEDYLKI
                     VEKSRQICPEISLTSDIIVGFPGETEDDFNQTHNIIKEIQFDDLFVFKYSPRPKTLAS
                     KWDDSVSQSEKENRHKIILDLQDEISLMKNKDLVGKILDVLCLKESQKKQGFLIGRDI
                     KRKVVMFKGEKNLIGKVVKVKITIGTRNYLLGELL"
     gene            complement(32758..>32991)
                     /locus_tag="PLW95_03815"
     CDS             complement(32758..>32991)
                     /locus_tag="PLW95_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21790.1"
                     /translation="IFLCGLIGTAIGEITLFFVPISSNFYNFLSSSFSPLSFSINNFN
                     LVVCSISLSIITKITPFTLVGIIAGCIYSLTKI"
BASE COUNT        11787 a         4470 c         5223 g        11511 t
ORIGIN      
        1 ttaatgtctc aacgaattag tatttcgagt agatttttat ttgacacaac agttaatatt
       61 tacctgaata tattgatatg gtataataaa ataaaaatgg aaatgaatat attaaaagtt
      121 aaaaaaaatt atgtaagaaa ttttgttagt tggcgtcctt catactaatc ctatccatat
      181 ctttcaattc tttatatcca taaaaaagga gatacaaaat gaaacaatta tttgaaaaat
      241 tattactaca aaaaacatta actatagaag aaagttatag tgtttttaaa aaaattatgg
      301 aaaatgaaat ttctccaatt caagcatctg catttctgtc tcttttgaag atgaaaggtg
      361 aacaggcatc agaaattata ggggcagtaa aattattaag agaaaaatca gaaaaaataa
      421 atataagaag aaagaaggtt ggagatacct gtgggacggg tggagaccgt tcagaaacat
      481 tcaatatctc aacagcagca ggtatagttg gttttggagc aggacttaca attgctaaac
      541 atggaaatag agcaattaca agtaaatgtg gaagtgctga tgttatggaa aaattaggtt
      601 ttaatatcaa tattcccatt gaaaaaaata aagaagttct tgagaaattc gggttttctt
      661 tcttttttgc acctttttac catcctgcaa tgaaaaacat tgctcctgtt agaaaggact
      721 taccttttag aacaattttc aatattattg gtcctcttac caatcctgtt aaaacaagtt
      781 ttcaaataat gggtgtttct gaatataaac ttttaagttt aattccgcct gttttcaaaa
      841 gtttgaatat tgatggtttt atattttttt ccgaagatgg aatagatgaa atttctttaa
      901 cagggaaaac atatattgtt gaagtgaata aatatggaat aagggaatat gaaatttacc
      961 ctcaaaattt tggtatgaaa aaatgttcta tcaatgattt aaagggaggt acacctgatg
     1021 aaaacaaaga aattattatt agaatattac aaaataaaga aaagggtgca aaaaaagata
     1081 tagttatttt aaattctgca tttcttttga aagcatcggg aaaagttgat aatataaaag
     1141 aggggattga attaagtaaa aagataattg aagaaggtgt tgcttacaaa aaatttatgg
     1201 aattagttga aaacttacaa aaaatatgaa aaaaatattt tttctgtttt ttgttttttt
     1261 tcttttaagt tctagtttag tggctgaagt agatttatat tttcaagcac cattagattt
     1321 ctgttcatat ccagatgtaa aagaaggagt tattgatatt atttatttaa aaattgatga
     1381 tttatggcac aaaggagaat ataataaaat ctttccactt cttaatttaa taacttttat
     1441 ttccccaaaa gaagagacag catgggaatt gggtggctgg tttttgataa atgcaattgc
     1501 tccttcttac aaaggagaga aaagagaaga aattgaaaaa tatggaattg aattttttaa
     1561 aaaaggaatt aaggaaaatc ctgaaagtta taatctttac tgggaaattg catggtacta
     1621 ttacaataaa ggtgagtatg aaaaaacatt agaatatcta aatgaagcag aaaaattcag
     1681 ttctgactat aaaataaaac atttgaaagg acatacttta attaaagtag gaaataaaga
     1741 tgaagcaata aaggtatggg aagaaataaa ggagaaattt ccagaataca gaaatgttgc
     1801 agaaagaatt ataaatgaat tgaaagagga tattaaaaat gattattgaa aaaataattg
     1861 aagagcaaat aaaaatttta gatattaaaa aaagagaaaa accaatagaa gaattaaaat
     1921 atttcaaaag ggagaaaatt gggttggtaa aaaatcttaa aaatagaaaa ataggaatta
     1981 ttggagaaat aaaaagggca tctccatctg ctggaataat aaataacaat attgatgttt
     2041 gtgaaatagc aaaagaatat gagaaattgg gagttgcagg aatttctgtt ttgacatgtt
     2101 caccatattt tcttggaagt attgatgacc tgaaaaaagc cagagaaatt gtttctctcc
     2161 cgattttgat gaaggacttt gtttttgatg aatatcaaat ttatgaaggg aatttttatg
     2221 gagcagatgt tgttctttta attttgagag ttattgatga taaaaatttt aagaacttac
     2281 tgaaaatatc tgaagatctg gattttgaaa taattattga agttcataat gaaaatgaat
     2341 taaaaagggc attttctctt gttgataact ggaagaataa aataataggt attaacaata
     2401 gagatttaga tactcttgag gttgacttaa atacaacttt aaatttgatt aaatttattg
     2461 ataaggataa aatacctgta ataagtgaaa gtggcataaa agaaaagaaa gatattttga
     2521 aattaaaaaa aattggagta aataattttt tgattggtga atcaatttta aaaagtgata
     2581 aaatagaaaa aaaattaaaa gagttaatgg aaggaggaga ttaaatatgg aaaataaagt
     2641 tattttgtca gaaaaagaaa tgccacaggc atggtataat attctgcctg atttaccaaa
     2701 accattacca cctgtattaa atccagcaac acaaaaaccc gtaacacctg aagacctgct
     2761 cccaatcttt ccgatgtctt taattcaaca ggaattttct cctgaaagat ggattgaaat
     2821 accagatgat ataaaaaggg gctatgctct ttataggcca actcctttga gaagagcaag
     2881 aaatttagaa aaggcgattg gaacaaaagc aaggatttat tataaagatg agtccgtctc
     2941 tccgtctggt tctcacaaac ccaatactgc aattgcacag gcatattata ataaaatgga
     3001 tggtattaaa agaattgcta ctgaaacagg agcaggacaa tggggaagtg cgcttgctct
     3061 tgggtgtaag atgtttggaa ttgaatgtat aattttcatg gtaaaaattt cttatcaaca
     3121 aaagccatat agaagattac tatctcaaac atggggagca actatgtatt catccccttc
     3181 tactcaaaca gaatttggaa gaaaagttct ggaacagcaa cctgattgtt taggaagttt
     3241 agggattgca atttctgaag caatagaagt tgcagtaaaa gatgaaaaaa caaaatatgc
     3301 acttggaagt gttttaaatc atgttttact tcaccagaca attgtaggtc ttgaaacaaa
     3361 aaagcaactt gaaatgatag gagaagaacc agatgtttta attggatgtg ttggaggtgg
     3421 aagtaatttt ggtggcttta tatttccatt tgttcaggat aaacttaaag gtaaagaaat
     3481 aaaatttatt ggtgttgaac caactgcctg tcctactcta acaaaaggtc cttatagata
     3541 tgattttgga gatactgctg gaatgactcc tcttttgaaa atgttcacac tcggacatga
     3601 ttttatccca gcaggaatac atgcaggtgg tttaagatat catggagcag cacctttgtt
     3661 atctcatctt gttaatttag gaattgttga agcaaaagca ttgccacaaa caccctgttt
     3721 tgaagcaggt gtcctttttg ctcaaacaga aggatttttg ccggcacctg aaacaaatca
     3781 tgctataaga gcagcaattg aagaagcaaa aaatgcacca gaagggaaag taatagttta
     3841 taatcattca ggacatggac attttgacct ctctgcttat gaatcatatt ttaaaggtga
     3901 acttattgat tatgaatatc cagaagaaaa ggttaaagaa tcgctaaaaa aacttcctaa
     3961 tatagaatag aagtaaatgt atcaaggaac tgcattgcct gaagagtggg aggaatgaat
     4021 ttaaaattga aaactaaaaa tgtaaaacta taactcaaag tttaaaattt ttttgatttt
     4081 ggttttaaat ccgccacatt cgccacaaaa cattgcgaat ccgacgcggt ttctggcgga
     4141 ctaatcgacg gacgctaaaa tacgcgcttt gtacctgaaa tttatctttt cccctgacat
     4201 ctttttactc tgttcctttt aaattagaaa tgacgaaaat aaagatatgt ggatttacaa
     4261 gaaggaaaga tatagaagac gcaattgatt taggaataaa atggataggt ttgaattttt
     4321 atgaaaaaag tccaagatat atcagtgaaa aagaggcgaa agttttgatt gagaaccttc
     4381 cttcagaagt aaaaaaaata ggtgtttttg taaatcctga tgagaaatta ttatttgaat
     4441 atgtaagaaa attaaattta gatggtgctc aattacatgg aaacgagcca ccatctcttg
     4501 ttaaaagatt taaaaaaatt tttcctgata aaattttaat aaaaggtata agagtaaaaa
     4561 acgaaggttc attaaataaa attaaaaatt ataaatttga ttatttactg attgatgctt
     4621 ttgatgaaaa attgtctggt gggacaggga aattgataaa ttataaatat cttgatagaa
     4681 aagatatccc atttgccagg atatttttag caggaggtat aactcctgaa aatgtaaaag
     4741 atataataaa aagattttct ccttatggaa tagatgttgc atctggagtt gaaattgcgc
     4801 caggaataaa aattaaagaa aaaatgaaaa aacttgtaga ggaagtgaga aatgcttttg
     4861 cctgataaaa atggaaagta tggtgaattt ggtggaaaat ttgctcctga aacattgatg
     4921 cctgctcttg aattacttga aaatgattat aaaaaatttt caaatgatac tgcttttaaa
     4981 aaagaacttt ctcaacttct ttctgactat acaggtagac ccactccact ctactttgca
     5041 aaaaatttaa gtaaatttct tggttttact gtttatctta aaagagaaga cctggctcat
     5101 acaggggcac ataaaattaa caatacatta ggacaggttc ttttagcaaa aaaaacaggg
     5161 aaaacaagag taattgcaga aacaggtgca ggacagcatg gtgttgcaac tgcaactgct
     5221 gcttctcttt taaatatgaa atgcgatgtt tatatgggga aaattgatat agagaggcaa
     5281 aaattaaatg tgtataggat gaaattatta ggagcagaag taatttctgt tagttcagga
     5341 agtcaaacat tgaaagatgc aataaatgaa gcaatgaggg attggatttc aaatctccaa
     5401 acaacccatt atgtaattgg ttctattgtt ggaccacatc catatccttt aattgtaagg
     5461 gatttccaat cagtaatagg gaaagaggca aagaaacaaa ttctacagaa agaaggtaaa
     5521 ttaccagatt atttagttgc ctgtgttggt ggagggagta atagtattgg acttttttat
     5581 ccattttata atgatatgga tgtaaaattt ataggggttg aagcaggtgg aaagggatta
     5641 aatacagata aacatgctgc aactttatgc aaaggagata aaggaatttt acatggaagt
     5701 tttacatata ttttgcagga taaaaatggg caaattcatc caactcattc tgtttctgct
     5761 ggacttgatt atccaggggt gggacctgaa cattcttttt ataaaacaac aaaaagagca
     5821 aaatatgttt ctgttacaga tgagtatgca cttcttggtt tccataaatt aagtgaactt
     5881 gaaggaatta ttcctgcttt agaaccatct cacgctattg gatatctcat aaaaaataaa
     5941 aaagaattta agagttctga tgttattgtt ttatgccttt ctggcagagg ggataaagat
     6001 gttgaagaag tcgcaaaaat agaaggaata aaatgaataa aattgatgaa aaatttgctg
     6061 aattaaaaat aaaagaacaa aaagcactaa ttggatattt tactgctggt tatcctgatt
     6121 ttgagacatc aaaacaggtg attttacgaa gtgtcagggc aggaattgat attctggaaa
     6181 ttggagttcc attttcagac ccacttgcag atggtcctat aattcaatat acttcaaata
     6241 ttgctttaca aaatggaata aacatggata aatgtttttc tctttgtgag gaaataaaag
     6301 attgtgtttc tattccatat cttttgatga cttattataa ccctgtttat aaatatggaa
     6361 ttgagaaatt tgcagtaaaa gcaaagaaat gtggtgtttc aggagtaatt gtaccagacc
     6421 ttccatttga agaaagtgaa ctcttgaaaa aacaattaaa gaaaaataat atttttttaa
     6481 taaacttttt aactcctttt acacaatttg aaagagggaa gaaaattatt gaacaggcag
     6541 aaggatttat ttattttata acaatagcag gtattaccgg agagaaaaaa gaatttgatt
     6601 ataatgcatt taaggttatt gaaaaatata aaaaaataaa agatatacct ttttgtgctg
     6661 gatttggaat atcaactgta agtcaaatta aaaaaattaa gaaatatgtg gatggcataa
     6721 ttgttggcag tcattttttg aaacaattaa tatatggtaa aattgaaaat gtagaaaaaa
     6781 gcgtttctaa atttaaagag gtgttattat gaaaatatta aaaaaaatta ttgctttagt
     6841 tattgtagtt ataattcttg gggctggatt tatttactgg cagaaatata cagaaaaaca
     6901 aaggataaaa ttagcagaag aagaaaaaag aaaacaggaa gaaattgaaa gagaaaagca
     6961 aagacaattc cttgaacaaa aaaaacagga atattattcc cttattgatg aaatgagaaa
     7021 gtattttagg caaggggatt ataagaaggt aaaggaaatt gcagaaaatt cgtttgcact
     7081 tgcaaaggac tttaactttg atacaactga aataaataaa attcttcatc aaattgaagt
     7141 aataaattac cagagaaaac tggaacaatt agataaagat aataaagata tatttaacta
     7201 ttcctatgtt agaaatgaat tattaaaaat accatcatta gaagaactga catcattaaa
     7261 aaacaaaata tatcagaaaa catttcagaa tgaatatctt gtattgcttg aattagcaga
     7321 aaaacatgca attgatggaa aacagggcaa aaattcaaaa attaactatt ttttaagtaa
     7381 ggactatctt tataaagcac aaaatttgag aaaaactaaa aatttaaagc aagatataaa
     7441 aagagaagaa aaaattgcag aaattcaaaa agatgtattt tttgctttta atcaattcaa
     7501 agaaaataca attccctctt ccctctacca gtaaatctaa ccacagggat taaatatagg
     7561 tctaataatt atgaaaagct atgaaaaaaa gatatagaaa aagtaaagag attattgcaa
     7621 aaaacatggg aacatcagtt attaaagagc aattactttc ttctgatata tacaaaagta
     7681 gaaagttttc caatattgga aatgttattg tctatatagg agatgtcatt aagtgcctcc
     7741 gtttattgcc tgatggtatt gtagattgtg ttataacaag tcctccctat tggaaacaga
     7801 gagattataa ggaccctcac caaattggtc aagaaagcac ctatcaagaa tatgtaaaaa
     7861 atcttgttgg tgtttttaca gaaatgaaaa gggtcttaaa gcccacagga acattttttt
     7921 taaatgtagg atataaatac cagaataagg aattgttact tatccctgaa ctattggcaa
     7981 ttgaactaca aaagaatggt tggactcttt taaacaaaat tatttggaat aagcccaatg
     8041 ctatgccatc ttcatttgat agtagatttt caaatgttta tgagccagta tttttatttg
     8101 tgaaaaatga aagtaaatat aaatattatt tgtcaataga tacactgaga ttgcctgtaa
     8161 ataattttgt caataataaa aatccagaag atatattagg ttttgaggta gaaaactcat
     8221 tattaaaaga caaaaaaatt aaaggatata tttccaaagt ttttaaaaat tccagaggtg
     8281 atatactcgc ccaggtgaat tgggatgata ggaaaaatac tattgagatt gtaaatgatt
     8341 ttaataaaga atcccagatt tcaattggtt taatttgtga ggattgtggt agaaccatga
     8401 aacatgaaat agatataaat agtcatctca attgtaaggg atttccaaaa cctatattac
     8461 ccccagtagc aaactttaat aacttaatag aattaagtga ttcatctttg ttttcttctg
     8521 tttctcctca attttttcaa aatagtagac gagtttacaa tggtaaattc aaattaagtc
     8581 ctgacaatag aggtgcttct cctggagcaa ggaaatcact atttggagaa tattttgttt
     8641 tacaaaggag atacgaaatt ttccaaccaa taattgctga ttatcttgca ttttggaaaa
     8701 gaaaaaaaaa catcactacg aaggaaattg ataaactatt aggatataaa gacacagcag
     8761 gtcattggtt tagaaaggat acaggtagtt ggggacgggg gggatctatt cctctgccag
     8821 atgattggtt taaactaaaa gaaatacttg aatttgataa tatctatgat agatggatta
     8881 cagaaactca tttagttctt caaacagtga gagcacaccc caaaggtaaa aatccgggtg
     8941 atgtgtggaa tataaaacta caaccttttt ctggtaatca ctttgctatt ttccctgagg
     9001 aacttgtcaa aaggtgcatt ttgtcaggat gtcctgaaca aggagttgta ttagacccat
     9061 ttgctggttc tggaacaaca ggcaaagttg cccaagaatt aggtagggat ggaatattaa
     9121 tagaacttgt tccaaagtat ttagatatta ttaaaaaaag atgtaaaaac attaaggaag
     9181 tgatttatgt taaatgatat tgaattagag aaaaaagaaa agcaatattt tgatttaatt
     9241 ctacttcatt taaaacagga cttaaattcc ctgatggatg gattaaactc acggataaaa
     9301 attctaaata actggtatga taactttata aaaacagcaa gaagtggtta taaatcatca
     9361 gatttggata ctggggcaga gagaattttt catcatttct ttgcttctat tttaaagttt
     9421 ccaaattctt ctcctgtggg ttctgacttg atgtttgaac ttccagattc ctttatgcat
     9481 attgaaatta aaactgctct tatagataat ccttcggatt ataaaggtaa aattaatatt
     9541 gctataaacc aaacttcata tagagtgaat aatgtatttt tacctaactt acctcaatat
     9601 tataaatcat catctttcaa tgaagaaaaa ccatgtttaa cttatattat tcagatagtt
     9661 cacaaacacg ctaaaccaga tattaaagca atcaaattag tatgtattcc taatggacaa
     9721 ctttacacac attatggaaa gagtattttt aaaagtggta agggtggtta tactaaggca
     9781 aaagattttc gttataatta ctctgctgaa ccatatttta aacttttatc aaaaaaatat
     9841 agagaaaaga tattcaggat tgagttagta tatttagcaa aagatataaa aagaaaggat
     9901 attactaatc tttcggatgt tcctgttcac tttcaatttt aagtttacta tgtttaaccg
     9961 tacaggttga ataaaagaat taacagaaaa ggcaaaaggt ctgtgttttt taaatgtaaa
    10021 aaacgatgaa tttttaaaaa ctatgttatt taaatataaa atttatgtaa aaaataatcc
    10081 ttataatatt tcactacaaa acataaagaa atatggaaaa taaagaatta gaaaatataa
    10141 taaaaaatag gaaacaatgg gtaaattctt caattgagaa taattttgat tttgatagta
    10201 ttttatctgg gttatatagt gacccatcta atttcatata tgaactatta caaaatgcag
    10261 aagatgaagg tgcaacagaa attagatttg aactctatga agattagata tttgtcataa
    10321 tgggaaagat tttgatgagg gggagagatt ttaaaactct gtacctaagt gtagcaattt
    10381 tccactaagg attaaaagtt ttttctctcc actctccact taaatatgaa ctgagttagt
    10441 gaatatttac cttgaagtta atcattacaa agggaggaat atggaaagaa cagaatttcc
    10501 aagacctgat tttaaaagag atgagtataa aattttaaat ggaatatggg attttgaaat
    10561 tgataaatct aatacaggaa ttgaaagaga attattttta aaagataaaa agttttccga
    10621 aaaaatagtt attccttttc ctccagagag taaattgtca aaaattaatg aaaaggattt
    10681 tatggtttca gtatggtata aaaaagaaat tgaaattcct gaaaattgga agaacaagag
    10741 aatattttta aattttggtg cagttgattt taaagcaagc gtatggataa atgaacattt
    10801 cttaggtacc catattggtg gatatactcc ttttagtttt gaaataacaa attacttaaa
    10861 aagaggtaaa aattacattg ttttaaatgc ttatgatgac aatcgactaa accttcaacc
    10921 tgctggaaaa cagtcattaa aatataattc ctatggatgt tcttatacca gaactactgg
    10981 tatatggcag actgtatatc ttgttgctac tggcaaaaat tatattgaaa aaatcaaaat
    11041 ttctcctgat attgaaaatg agaaggttaa cttttttatt gacacaactg gtggtaaatt
    11101 tttagaattt gaaatatact tttcaaatga aaaaattggt tttggtaagt ttaaagtaga
    11161 caaaaatgga atatattcag tgcctataaa taaaataatt ttttgggatt ataaaaatcc
    11221 agcattatat gatgttaaaa ttaaactttt tgaagataat aaaactttgg tagatgaaat
    11281 ttatacttat tttggtataa gaacaataaa aattgcgggt aataggatat atttaaatgg
    11341 tgaaaaatta tttatgaaaa tggtacttga ccagggatat tatcctgatg gaatttatac
    11401 tgcaaaaaac caggaagaat ttaaaaaaga tattttaatt tcaaaaaatt taggatttaa
    11461 tggggcaaga ttacatcaaa aaatttttga cccaatttat ttgtattggg ctgataaatt
    11521 gggctatctt gtatgggagg aatatccaaa ttggggaata aaaaatcgca ttagtgaaga
    11581 aacatttcat attttattgt acgaatggat ttcttcaata gaaagagata ttaatcatcc
    11641 ttctataatt ggttggtgtc ccttaaatga gacaactata tttcagaact cagaccttgt
    11701 aaaaactcta tatagaataa caaagaagtt tgatattaca agaccagtga ttgatacaag
    11761 tggatatatt catgttgaaa cagatattta cgactgtcac aattataaac aaaatccaga
    11821 agaatttaaa aatgattttg aaaaatttaa aacacaagat accatttgga aaaattttcc
    11881 agaatatgat gcagagtata aaggacaacc ttattggata agtgaatatg gtggaatatg
    11941 gtggagtgaa gaagaaagtg ggtggggata tggagataga cctaaaaata aaaaagagtt
    12001 tcttgaaaga tacaatggtt tgacaaaagt tttattaaat catccgaaaa tatctggttt
    12061 ttgttatact caattatatg atgtagaaca agagaaaaat ggactttata cttatgaaag
    12121 agaagtgaaa tttaatccag aaattataag aagaattaat tctggtttat aatttatcaa
    12181 tggaaatact ttcaaatcat ccctctttac agtctaatat ttattgaata tcaaaaaatc
    12241 tgcatttact caatctgaaa ttttcctctt gaaattaaag atgtttatca aatgtgatat
    12301 aataaaacaa tataaaattt aagaaaatag gtgaaaaaat gaataaagat gaaataaaaa
    12361 tagggcttgt tggatgtgga actgttgggt ctgctttttt gaaagaaatt attaagaaaa
    12421 aaggtgagat aaaaaagaaa acaggactga atattacatt aagtaaaata tatgataaga
    12481 ataaaaaaaa gaaaagttta ctgccagaaa aatttgtatc ttctccagag gatataatta
    12541 atgaccctga aatagatata tttgttgaac ttattggagg tatttctcca tcttatgaat
    12601 atgtagttaa agcaattgaa aataaaaaaa gtgttgtaac agcaaataaa gcattaatat
    12661 ccgaaaaggg gaaagaacta tttgaaaaat caaaacaggc aaatgtttat attggttttg
    12721 aaggaagtgt tggaggtgct attcctgtaa taaatatttt aaaagaaagt tttattggga
    12781 ataagattat gacagtaatg gggattttaa atggaactac aaattatata cttacaaaaa
    12841 tgtatcatga agggattgat tatgaacagg cattaaagga tgcccagaaa aatggatatg
    12901 cagaagtgaa cccatctctt gacattaaag gtatagatac tgcacataaa cttgcaatac
    12961 ttacaactct ttcatttaat aaatggattg attggaaaaa aataaaaatt gaaggtatag
    13021 ataaaataga aattgaagat ataaaatttg cagatgaatt tggatataga attaaacttt
    13081 tagggatatc aaaaataaat aattctaaaa aattggaatt aagagttcat cctgttttaa
    13141 tttctaaagg tcatttatta tctcttgttg atggtgttta taatgctgta tactttgaag
    13201 gtgatatgat aggaaaatcc cttctttatg gagaaggtgc aggaagttat gctgctgcaa
    13261 gttctgttat atccgatgtt attggtattt caaagaaaat tgtaaataat aataaaaatg
    13321 ctgatatttt atggactaat agagaaattg aaatacaacc atttcaggaa cttcaaagca
    13381 gatattattt cagatttaca gcaattgata aaccgggggt attgtcaaaa atttcaaaag
    13441 ttttaggaga aaacaatata agtatttctt ctgttataca aaaagaggaa aatccccaaa
    13501 aagcagttcc tattgttatg ttaacacata aagcaattga aaatgatgtt gagaaagcaa
    13561 taaatggaat aaataaactt acaataatta agaaaccaac tgttgtaata agagttgaag
    13621 aataaattcc ccctcactct accctctgca caacggggtt gaggggattt tctcctactt
    13681 aaatattaat atttactaaa atggaaaaaa tactttatag aagcactaat ggaaaagcac
    13741 caattgtgag ttttaaagaa gcattactta atggacaagc acctgattat ggtctttatg
    13801 ttcctacttt cataccaaag ataagaattg atgaaataaa atgtttgaaa caaaaaccat
    13861 attttgaagt tgcctttcac atactttata aatttttaaa aggagagata ggagaaaaag
    13921 aactttttga gatgtgcaaa aatgtatatg attttgatgt gcctataatt gaaattgaaa
    13981 aaggcaaatt atatattctc cgtttggata aaggacctac ctgctcattt aaggattttg
    14041 cagcaaaaat gatggcatca cttatggaat attttgcaaa agaggaaaat aaaaaattaa
    14101 cagtacttgt tgcaacttca ggagatacag gaggggcagt tgcaaattca ttttataaga
    14161 aagaaaatat aaaagttgtt gttctttttc ctgttgaaga ggtctctgaa aggcaaagaa
    14221 aacaaatgac aaccttaggg gaaaatattt tacctatagg tatcaagggg aaatttgatg
    14281 attgtcagaa ctttgttaaa agagcattta atgatatatc tctttcttct ttgaatttaa
    14341 catctgctaa ttcaataaat attggtagat tacttcccca gattgtttat tatttttatg
    14401 catatctaaa atttgaaaaa gaaattgttt tttctgttcc aagtgggaat tttggaaatc
    14461 ttatgggagg ggtaattggt aaaaaaatgg gtttgccagt aaaaaaattt cttgttgcag
    14521 taaatgaaaa taatgaattt cctgagtttt taaggacagg tgaatatatg cctgttattc
    14581 cttctaaaaa atgcagttca aatgctatga atgttggaca tccaagtaat tttgctcgtc
    14641 ttattgacat ttatggtggc tggataatgg atgaaagaga taaagatgga aaagttataa
    14701 aatatggagt aatgaaaagg aaaccagata tggaaaaaat gagaaatgat tttatttctt
    14761 tttcaatatc aaatgaagaa gtagatgaga caattaaaaa ttactatcaa aaatataaaa
    14821 tagttattga gccacatacc gcagttggaa taaaaagtta tgaaaaaagt caaattagtg
    14881 atttggtaat tgttcttcaa acagcagacc cggcaaaatt tccagaaaag ataaaacaac
    14941 ttttaaatat tgaaccaaaa ataccagaag cattagcgaa aatactcact aaaaaagagg
    15001 attcatttga tgttttagaa aataattatg atgtgtttaa aaaatatctt ctggataaaa
    15061 taaaatgaaa tacttaattt taataatgga tggagcagca gattacccta tagaaaaatt
    15121 aaatggtaaa acacctttac aggtggcaaa aaagataaat atagataaat tagctgaaaa
    15181 aagtagatgc ggtcttttaa aaaccattcc tgaaaatttc tcatctggtt ctgttgttgc
    15241 aaatctttca atacttggtt atgatcctga aaaatatttt aaaggaaggg gagttcttga
    15301 agcagcagca atgggaataa aacttaataa aaatgatatt gcttttagat gtaatataat
    15361 caatgtttca gaaaataaaa tagtaagtca ttcagcaggg cattttgatg ctgatgaagg
    15421 gaaacaattg atagattcct taaatgaaaa attaggtagg gaaaatatag aattttattc
    15481 tggtgttgat tatagacatg tacttgttat gagagaaaaa tattcagaaa aagtaagatg
    15541 ttatcctcca catgattttt taggtgaaaa tttttattct ttattaccaa ctgaaactga
    15601 aaaggaagga gaagaaacat ctgaaatttt aagagaaatg attgagaaat ctaaagaaat
    15661 tcttgaagat catcctgtaa atttaaaaag ggaaaaagca ggaaaaatga aaggcaattt
    15721 aatgtggcca tggtcacctg gtaaaaaacc agacatggag acatttcagg aaaggtttaa
    15781 cataaaagga gcggttattt caggagttga ccttataaaa ggtattggta tttactgtgg
    15841 ttttgaagtt ataaatgttg aaggagcaac aggtgattat agaacaaatt atgaaggaaa
    15901 agcacaaaaa tcaattgaat cactaaaaga atacgatttt gtttatgttc atgttgaggc
    15961 gcctgatgaa gcaagtcatg aaggaaatat tgaattaaaa attaaatgta ttgaggattt
    16021 tgataagcga cttgttggta atattttaag gaatatagat atttcagata tttcaatagc
    16081 aatattacct gaccattata cttccgtaat tacaggaaaa catactggtg aacctgttcc
    16141 ctttcttata tataaaccag accagttacc agatgaagta aagaaatttg atgaaataag
    16201 ttgtaaaaat ggtttttata aaattcttga aggaacatct tttatttata aacttatttc
    16261 ataaattttc aatggaaatt attatgtcag gcacaaaagg acaaagtaat caagagtgca
    16321 aaatataaaa caaaaatata aaatgaaata agaaaaagcc ccctcaccct ccctctcccc
    16381 acaggggaga gggaaagaag agaggggaat gtatttcctt ctccccttgg ggagaagatt
    16441 aagatgaggg ggaaatttga catatcttgc tacttgcatt aaatccacct tcatcccttt
    16501 ttgctaaagg ggggacattt gtctccttcc tttatcaaag gaaggtgggg atggatttaa
    16561 aaagtatttt ttctctactt tttactcatc actaaaatat gaacgaagtg aagtgatact
    16621 aaattagagg tataatttat ataggataga aaaatgtaca ttataatagt tggatgtgga
    16681 aaaacaggta agtatctggc tgaaatttta caggcagacc ataatgttgt tgtaattgat
    16741 aaagaaaaag aagcaataga taatttaggt gaatatttta atggaattac tattttaggt
    16801 gatggtatgg atatagatgt tttaaaagat gcagggattg aaaaagcaga tgctcttgca
    16861 gttacaacaa gtaatgataa tacaaatgtt ctaatagcac aaattgggaa aaaaattttt
    16921 aatttaacaa aagttgttgc aagagtttct gcccctgaaa aagccgaatt atacagaact
    16981 cttggtgttg aaacagtaaa tagcactgca atttttgctt ctcttataag agataaaatt
    17041 attgaagaga aattttctgc ttatattctt gaaagcagta aacttacaac tattgagata
    17101 ttaacaaata gtaaattagc agggaaaaaa gtgaacgaaa taaacatacc aggagaattt
    17161 cgtataatta caatagttag agaacaagaa ccaattattc cagatgatga tacagaaata
    17221 caaaaaaatg atacaatagt tgggcttata aaaatcagta gtttgaaaaa gttaaagaga
    17281 gagttagatt tataaaatga atatactaat tgttggtggt ggtaatatcg gttattatct
    17341 ggctgaaaga ttgagtggga gtcattatgt tgttcttatt gagaaaaaat tgactgttgg
    17401 agaagaaatt gcaggtaaaa caaatgtaat ggtaattttg ggagatggat gtgacccaca
    17461 ggtacttaaa aaagcaggta ttaaaaaagc agatgttgta gccgcagtaa caggaaatga
    17521 tatagataat ataataatat gtcaattggc aaaagatatt tataatgtgg aaagaacagt
    17581 tgctattgtt aataatccta aaaatcaaat tgtttttaca aaattaggaa tagatgttgc
    17641 tattgatagt acagccatta ttgcaaagat aattgaagat gaaattggat gggaggactt
    17701 tgtaaatctt tttgcattta aaaaaggtaa tttatcactt ttgagaattg accttccaga
    17761 gacatcacct attattaaca tgccaataaa agaactaaat ttaccagaag attcagttat
    17821 tgttgcagtt ataagaaata ataaaatact tgttccaaca caggacttta ttttaagaga
    17881 gaaggatgaa ataataacta ttacaaaagc aggaaatgag tcagacctgt ttaaaaattt
    17941 aattggagag attgaagagt gacaaataaa ttttcatttc tgatgccgag atttcaaata
    18001 aaaaaaatca caaagtattt aaccatattt ttaaatggcg ttttgggaat atttattgaa
    18061 attatatata cacttggaat ttttttactc tcttcaatta tttgtttctt attttatata
    18121 cttataattt tttaaaaaaa tgataccgca attttcaaga gatgatttta aagttgtaat
    18181 ttattatatt ggcaatttaa tagtatattt aggtattatg atgttattgc ctctttttgt
    18241 tggaattata aataatgaaa taaatccttc tcttgatttt atcatatctt ctttattatg
    18301 tttatcaatt ggtaatttca ttatgtataa atttccatat aacccggata taaactggcg
    18361 acagggaatg agtattgttg ctctttcatg gattgtctgt atgtttttag gagcaattcc
    18421 tcttttttta tcagggcatt atatcagtta tttagatgca tgttttgaat caatgagcgc
    18481 ctttgcaact actggtcttg ttcttgtaaa tgaccttgac catatggcat atgcttacaa
    18541 cttctggagg cattttatgt gttttattgg tggtcaggga ataattctgg ttgggcttac
    18601 attttctgtt agaggtggtg ctggtatgaa agtttatgta ggagaaggta gagaagaaaa
    18661 aattttccca aatattgttc agacagcaag atttatatgg gcagtcagca ttatttatct
    18721 ttttttagga acacttgttc ttttttttat atgtcttttt cataatataa ctcctggaag
    18781 agcgttattt cattcaattt gtatatttat ggctggatgg gataccagtg gttttgctgt
    18841 tcagagtcaa aatattcttt attatcatag tgcagcaata aaccttgtta ccactcttat
    18901 atgtgttctt ggagcattta attttgcagt tcattatact gtctggactg gtaagataaa
    18961 agaattagtt aagaactttg aattaaaatt attttttggt agcattattg ttctttcgtc
    19021 aattgtatgt ttagggtttt tagggcattt ttcattccca aacttttttt ctttttttag
    19081 attaactttt tatcatttag tttctgctca taccggtgtt ggttatacta attcaactat
    19141 ttatcatttt ttgaattatt ggccatcttt ttcattgata ggacttatta ttgcaatggg
    19201 tcttggagga tgttcctgtt caaccgcggg aggaataaaa atcattagaa tagggatttt
    19261 ttttaaagga ataaaaaaag aaataaaaag attattattt cctgattcag caatattggt
    19321 agagaaattt cataatataa gagatgtttt tattcaggac aatcaagtaa aatatgcaac
    19381 aaatataata attttatata ttctttttta ctttattgga gcaatggttg ggacttttta
    19441 tgattaccct tttttatatt ctctttttga gtcggtatct gcatgtgcaa atgttggact
    19501 ttcagtcggt attacaactc cttcaatgcc aactttatta aagataacat atattttaga
    19561 aatgtggttt ggccgacttg aatttatttc aatttttgtt gttattggtt ttattttttc
    19621 attaaggagt ccagaatgaa aaaaattttt aattttgtta tatttttgct aattttttgt
    19681 ttttctgctt ttgcacagga agaaataact ggcaaagacc tttttgaaaa aatgaagcaa
    19741 ttagataata aagaagttgt tattgttgga gagtgcattg gagatattat taaaggtgat
    19801 aaaaacacat ttattgtaaa tataagaaca gatgataatt attttatagg tactgtttta
    19861 agtaaagaag atgcagagaa aataaaccat ttaggaaaat atgaagaaaa aggagatatt
    19921 ataaaaatta ttggtatttt ccattctaat tgtcctgttc attctggtga agttgatata
    19981 cattgtgcaa atatggaaat tttaaaagaa ggagaagttt atgcagaaaa aattgatatg
    20041 aaaagattta ttgtaggtct tgttctttta tttattatgt tattttttac taaactatct
    20101 tttactaaat ccgtataaac tcgtatttta aaaaagtttt ttcatttaca ataactaact
    20161 ggttcataat caccaacaaa agcagttgta tcagatttta cagtttcttt ttcattatcc
    20221 caatcacttt caactgtttg agagacaatt cttggtttta tcttacattt tctacatccc
    20281 gaaaaacctt ttatttctcc tttcaaaaat ttttctataa cttgtctaca tactttcata
    20341 tttagaacaa aaacttgagc ccctccaatt ttcccttctt ttaataattt gttttttctt
    20401 atgtaatccc aatatgctcc tttatcagta atttcacaag ctcttcctcg ccatccccat
    20461 gttcttatgt gaacaatttc tcctgaagga agttttacag gatattgttc tgttcttttt
    20521 ccaaggcaag gaacattatt tgtttgttca acaacatgat gaaatgtatt tgctgataac
    20581 gcatcaataa gtataatttt acctccctcc ttttcaagaa gataaagagg acttcctttt
    20641 cccatagtaa ggtatttatg atggttttta acaaagtcct ttgcattttt tccccaaaca
    20701 gcaaaagaat gagttggatt aagagaacga taaacatctt ttattttcca aaatgcttct
    20761 ggcactgccc cattagttgt tggtgtctca agaggtgaga aatatgcatc tgggtcattt
    20821 ctaaaaattt caccgtgatt aaaagaaggc atcattaaaa ctccttcttt cccaataagt
    20881 tccataagtg cctcacatac tgcctttgct ccaccttcta catatccaag agaagaaaga
    20941 gatgaatgaa caataacttt atctcctttt ttaattccaa gtttttcaag tccagatttt
    21001 atatcctgtt tagtaacaat tgttttataa taaaatttta aataattata attagataaa
    21061 tactgaatat attttataag ggattttttt tctgcttcac ttatctcatt cattccataa
    21121 aattcccttt cagatgaaat ttctttgaat atttgataca tatctttttt accatccact
    21181 ctattgataa cagaataaaa ttcttctggt ggagaaattc tttctttatg aggaatttct
    21241 gctaaagaaa aaataaatac tggttttctc catgttataa caatattttc acttttttta
    21301 tcttctcttg ataatagaac ttcttttttt gaaatttctt tatcaaattt tttagtaata
    21361 tcagttatag tttttctaac tatcttgtct atttccttta aaattttttc ttctggttta
    21421 tcaccaaaaa tttttattga gagatattta tcttttaagt aacgagagat ataatttatt
    21481 ctttctttaa ttccttgttg agtaaatttt tcaggatttt ctgctatcat taagttaaga
    21541 taacttatat atttataaaa ttcctctttc acagaggtat taacaacatt tagatatttt
    21601 tttcttttct gatttgaaaa aagagtacag acataagttg ctaaagaaca taaaatttcc
    21661 tttcctaaat tccagttaac aatttcaggg tcagcagaat tatgatgaaa ctttccagga
    21721 tgtgatataa aatacattgt tggaatatta gttgatttat cagatataaa tgtatcatct
    21781 tcaaacattg ctctttcttt tttccatggt aaattagaag ggaatgtttt ttcaagaagt
    21841 ttttcaaaga ggtagtcacc aaaaaatgga tttgtaaaat gacttccata cagtgttatc
    21901 ctcattctac ccgatttaac aatatcagga gtaattccat ctacattaaa tgcatatatt
    21961 acattatgtt ttttttcaaa aaactgagcc attccatatc tttccattga aaaaagaaat
    22021 cttattccaa catcaggaat aaattttttt gacttaataa gttcatttat aactctacat
    22081 atttcaatca atccagcaac accggtcgca ttatctgtaa tcattggttc ataaagatgg
    22141 gcaagaaaag caatttcttt ttttgttttt cctggaataa gtcctgtaat tgaataaatt
    22201 ttcccattgt atcttttgct tttcacagag gcacagacct tcacattttc cttatcaagt
    22261 aatttttgca ggaattcacc atcttctggt ttaattgaaa aacataccat ttttctatct
    22321 tcttttgttt gataccaacc agaatttttt gcccatccat taatccaata tactccatct
    22381 cttttttcag gagcaccaga atatgaagag ataattccta aagcacctgt tttttcaatt
    22441 tcgtttctac attcccttgg gggaatattt ccacagaata caaaattctt acttaaatct
    22501 ttatattttt taatatttct tatatctact acatccaaaa ttgtttcttc aatatcacca
    22561 catctatttg caatatgaaa tggatggtgt tttgtatcag caagaatttt tccattaaac
    22621 ttatcaggtt ctttaatata aagaaatcca tattcataat cccaagcctg aggcataatg
    22681 aaatcaccat aagtggtccc accgtctgct ttgagttcta ttagttcaac atctttcgca
    22741 cctgcatctt ttaattcctg ccaacataac tttgctgact tttcaaaatc actataacaa
    22801 aaccttgatt caatattaaa tattttcagt gctaaattct gccatctact taaatcaaat
    22861 tcttttttta tccttttatt aagattttcc attttttatc ctttgctctg aattgatttt
    22921 gccaaaattg gctatatctt ttatatattt tttcttcgtt ttttaatgct tctataagta
    22981 tttatactaa tttgatgtca aattaatatt atacccctcc tcttttgaaa tatccccctc
    23041 tgggcgtagc ccctacgggt gagccttacc cctctttata aaatccctct ctatctcctc
    23101 caaaatgcgc gtcggacaga cccttatcaa agggagaaat ttaaaatttt cctcatcctg
    23161 catcctcggg cgtagtccag aggggatccg tagccaaaag cgaggaccct acgggcgagc
    23221 cacccttccc ctctacaaag catagacggg caggctttca aaaaggaaag agttaaaggt
    23281 cacctaactc ctgttgtaag tgacaaatat gtttttttcc tgtagggata agcccttttt
    23341 ctctctacaa ttacaaaagc atttttctaa ttttcatctg gttcagtgct aatgtccaga
    23401 attttctcta ttatttcttt ctccatcgga atagtgccta taccacctgt aattttttcc
    23461 cttataacag gcacacttac caattgtcca tctactaata tagccagttt tttccctatg
    23521 agtgattctg ttaagttagc aaactttttc ctaccttcat ctgtaaatac tattttaatt
    23581 tgttcaagct cttgattagt caagcatgct ctgacacctc ttatatccga cttatcaatc
    23641 tcactcgtat ctgcaacata tattttttta tcagaataag gtatattggt ctgtgtaaat
    23701 cctgtttctg gggtgttttt cacaagtcgg aattctatac ttctttttgg tttaacatat
    23761 cttgttgcct taatcattgt aagtgtttta agggtatttt ttggagaaat catttcagga
    23821 ctgtcaatca aacctcctat tattgcaggt ttttcgtttg taatgtccac acaggtactt
    23881 tgttctctat tactcataat agggtatatt tcttctccct tcccttcatt ttttattttt
    23941 ttcaattcta caagttttgt tgttgatata tctatttgac agcgtatttt gttgtctttt
    24001 attatttcgg ggattatttc aaataacatt ccttcttttt ctgttgttcc cggaatatcc
    24061 tttctcatat ccagaacaca tttcttccct tcatcagcaa aaacttccac ctgtattatg
    24121 tccagaatac cctgttgttt gagagaatat aaattcgcat acaatttctc tacatcaacc
    24181 tgaggaaaag tttctggtgt tccgggagtt tttattaaaa atatttctgg tagttctgtt
    24241 attaccttta tttcaatttg ttcaaatttt attatttcgg gacttgcatt atcacttttt
    24301 cttgatatat ctcctctgga tattttattt tctcctattt ctgaaaataa aggagcgtaa
    24361 gcactaattg gtatctctgc tcctttatct tcccagacaa tgtcatagtc acctttccaa
    24421 ttcatttttt ttgcaggaac tccaccatca tctttctcat tttctccaac actatacaca
    24481 ataaatccct tatccttctt tttgtaaatg tagtccttac cgctaaaagg gtctaatggt
    24541 aaggaagata aaatttcagg agtgagttga tttaaagaat ctgcaaactt tccatgttgt
    24601 tgtctgtaaa tgcgattagc caatccaatt tcagcagtcc ctaaataagc atcaagattt
    24661 gcttcttgaa gataggcacg agaaacagca agacatgttt gcttagcaag aactgctctt
    24721 ttctcgggta ttttttgaat ttctgtatta aggtcttcta tctttttttt aacttcccag
    24781 tagggttttt cagcaaaaga aattatttta atcatgttct caatataaac agatatttct
    24841 tcatcccaat atttctctgc aaatttttga attttttcag ggtcatcagt gccaaagaga
    24901 ttttttgcat attctttttc ttcttcactc atttcttttg atttttgctc caagtcaggt
    24961 agtttctctt ttactgattg ctggtactga cgcattttag gtagaccaaa taaaatcatt
    25021 tctcttttta atgattctga aataatttta cattttcttt cctcttccat attttgaata
    25081 agcgtttgat ataaactgat atttacttct ttactgtctg gaatttcttc taattgggac
    25141 agaacgaagg tatctattgc taacctgact aattgggtta taagtaaagg ttcgttagag
    25201 agtgatttac ctaattttag agcaaccagt attgtattta aagatttgtt gatgtcttta
    25261 ttttctgctt caagttttgc ttttgcacat aacatacggg tacaacctct taaattggca
    25321 aggatgttta aaaacaacaa agagggttca tccgttaatt tatcaggcat caaaaattga
    25381 catttcattt gagatgcttt ttccagtagt tgatacattt taccaaattc agggttatgg
    25441 agaattaaat caaccacttt tttcttctct tcttctggaa catcttccca atctactatg
    25501 cctgcgtatg gaaaatattt ccattcctct ttgtattttt ctttaaggga attcatcagg
    25561 acaaaaactt cgttataaac aacagcacca ttttcttcat cggggatttt aggcaagttt
    25621 aattcttcta ttgtggtggg tattcctgcc tcttttattt tttgtagttc ttcttccaga
    25681 gatggaatag attttacttc tgttgctgaa gaggcaaaag tggagaacaa aaaaaggaat
    25741 aaaaagagaa aaaaactttt ttgtttcata atatcccctt ttatttaatt taccattatt
    25801 tcaagaattt tgaaataatt ttatttctga tggcaacaat gaatttaaag aacaaaaata
    25861 taaccagtgt ggatataaca ataattaaag atatatatat ccataaaggg aaatgcttac
    25921 tatataaaaa accaaacagt gcaacaagaa aaaagagtaa ataaagccct attataattg
    25981 taattcctac aaaaataatg ggtattgctt ctttttttat ttcctgttta tgcagaacaa
    26041 tatcgctttt tattgatttt ccaacatctt tcttgtttcc acatatttta caagtatcag
    26101 tattaccagg attatcataa ctacactcat tacataccca tatttcaccc ttttttattt
    26161 tgatttcctt ttccgcaagt tttattttgg cagatagtcc aagaagtcct ggtatatgac
    26221 ttttttcagc tatttcattt gcacgcttgt agcagtcaat acctttttca aatttctctt
    26281 tttttatata taaatctcca agttcaacaa gtgctttgta aagaagagca ggttctttgg
    26341 cattttctat aatgtttaat aaattattta tttcttcttt ttcctggagt aaagcattta
    26401 atttatttgt cttatcttca tactgtttca taagaaacca tattatcaaa ggtataggaa
    26461 ttgcaataaa ccccacaaaa taaagtcctt taactacttc aaagattgta agaaaaataa
    26521 gagttcctac tattatttca attatataca attgtccatg tgttatatcc ccgaaactaa
    26581 accaatacca gaaccaccat ccttcaaggg taagcaaaat caacggaata aagatacaaa
    26641 atattgtaat aatatccata tctaccttct ttatgtttat ttattaaaca cttttttctt
    26701 tacctaccag agaaacaaat atatcttcaa gtgtcaggtc aataacttcc atactttttg
    26761 gaccaaattt ttcaagtttt tttaatatct caggggtaaa gttttttatt atcataacta
    26821 actcatgtcc tataactttc tttttcaata gtttatcatt tttatcatta aaaaaaattt
    26881 cttcgggtat cttatcttca aaaataagat ggatttcttt tactgaattt ttcagtgttt
    26941 ctaatggaca ggaaagtaaa agttttccct cttcaataat acctacatag tcggctatct
    27001 gttctgcttc tgtgataata tgagtagaaa agaatacagt ccttgtttcc agatgtatgg
    27061 tttcaataat tccctgaaga aattctcgtc tgacaacagg gtctaatcca gaagtagggt
    27121 catcaagaat taaaagttct ggttggtgag aaagtgccaa taagagggca agttttccgt
    27181 tcattcccct tgaaagttct ttaactttta tttttttagg aagttcaaat ctattgagca
    27241 ggtcttcagt taaattattg ttccaggtgg aatagaaagg acgacagaag ttaattgttt
    27301 cttcaatagt catccagtca tacatcttct ggttatcagc cacatatcca accctttttt
    27361 ttatttggac tggttctttt tctgggtcca ttctaagaac tttacttttt cctgatgttt
    27421 tttgtagtag ccccataagc attctaatag ttgttgtttt acctgctcca tttctgccaa
    27481 gaaaaacata aactgacccc ttaggaacta ataaatccaa tgattttact gcttctttat
    27541 gttttatgta cctttttgta agtttctgtg tttctattac atattcttcc attttttatc
    27601 tcctttcaaa ccctctcttc tcttcaaagg gagatggagg attttacatt cttttcttat
    27661 ctttttctaa aatccaccct gttcctttat cttccttcct tttataaagg aaggttagga
    27721 tggattttcc tgtatttcca cttataaatc cccctaaccc cctttttcaa agggggaact
    27781 tgtgggtaag ggatagatcc ttttataaag gagggagtta atattaaagg gggatttgtc
    27841 ttttcttatt taatttttct acctcaatac cttaaaagaa atatcatcag agttttgttt
    27901 cctatcagat ataccaccat catctttctc attagaacca acactataca caataaaccc
    27961 cctgccctct ttcctgtaaa tgtagtcatt tcctgtaaat gggtcaattg gtatttctgg
    28021 tataatatca ggtacaagag atgaaagtgt ttctggataa tacccatatt tttctttgta
    28081 tatttttaat gccaaagata attttaatgt atcaagacag gcattataac tactttgctg
    28141 ctcaatactt cttgtaatgc taggtaggaa tattaaacta agtatgtgtt tactcatact
    28201 aaatgtatta taggtaaaaa acttcctgtc cagttcgtca agtttatttt taactaaata
    28261 atacggtttt tgtgaatagt caattagttc taaaaaatat tgaatataaa aggcatagtc
    28321 attttttaaa attggttggg ttaaataact gccaactaaa ctaccataaa tcccaggaag
    28381 ataagtattt tggcggttta atatatttct taaagaatat ccaaaaggaa tatctttctc
    28441 aaaaacatat cttccaaaaa gtactaattc tccatttaaa cttctggtaa tttttctgtt
    28501 ttttttgtct attattgaaa ttaagtcata ataattatct tttgaaaaag aaatgtcttc
    28561 tttgttaaga agataattaa gggaattttt tgctattaca tctattgcta tacgaaccag
    28621 ttgggaaata ataattggtt catcagttaa tgactcacct atttttagcc ctgtcttaac
    28681 tgaatttaat gcatcattgt attttttatt ttctgcaagt atatatgtcc ttgctgctac
    28741 taaccttgaa cattgacgca ttttactaag gtggggcagt aacatagcag gaccttctct
    28801 atatttaata tcaaatctac aggaaggcat gttgactgct ttatcaatta aggtatagaa
    28861 tttaataaaa tcagggtctt tcataatctt ttttatattg tttttctcct gtgttgttaa
    28921 ttcttcttct tttttcatcc cctcataagg catataatac cagtcagttt tgtatttttc
    28981 ttttaattta tctactaaag taaatgtttc ttgataaaca atagcagcat ttttctcatc
    29041 tggaacaggt ggtggtatta cttcttctgg cgttaattta atcccttctg cttttgcctg
    29101 ttggatagtt ctattaaaat ccctactgat tgtaatattt acaatactat gacaaccaaa
    29161 agagacaata agtattataa ttgctgttat aaatattttt ttcccacatc caacttcttt
    29221 tacaagacat ttctgccata taacaagact aaaaataaga aagacagcaa tagaaacaga
    29281 aaaaataaag aatatgaaaa taaacttggt aggagatatt aaaaataaaa agacaaccag
    29341 aggaattatt agtaaaagcc caaataaaag aagaaaagga gtgcaaatta ttgcaggaag
    29401 agtattttta agcagtagtg aactgaaaca ggaagaagaa taaataatga taaaacagag
    29461 taaaagataa atttttataa gaaattgttg ttgtggtgca gtcagaatag tccctgcttt
    29521 aataaaattt ggaaagggag agaataaaat aatgttggtc agaagagtaa gaattattaa
    29581 aagaaagaga ccaaaaaagt attttgtcca gaaaattcta acagtagaaa ctggttgact
    29641 taaaagaaat tcttttgtgt ttctataaaa ttcatttgta aaagaaattg agccaaggaa
    29701 taaagaaaaa agtattggca gtatagtggt tatagacaaa atatagaact catagtcaat
    29761 cctatattca aaaaattttg gaattattac tggtataatc ctacataaaa taataattcc
    29821 aatagataga aagaagaaag gactttgttc tctccattct ttccagatta gtgttttcat
    29881 tttttattcc tccttgtttt gagttgattg gtaaagagtt gaataatctc ttcttctgta
    29941 aatcccaaaa tagttgcctg atgaattgcc tggtcaaata tctcatcaac aatctttttt
    30001 ttctctgatt cagcaatttg ttttacatca tctgatataa atgttcctcc accccattta
    30061 cttgttataa ttttttcata ccgtaattct ctgtaagcac gggcaacagt atttggatta
    30121 atacgcaact gcatagcgag gtctcttata ggtggtaatt ggtctcctgc tttcaaagca
    30181 ccgcaggcaa caagatattt aacctttttt ataatttgca tatatggtgc tactccacta
    30241 tttgaatcta tttttatata cattttatta gtttattgta ttaactcttt aatacaattt
    30301 tacctcaaaa aattatatag tcaataataa ttatttttat tttataaaaa gaatttcaac
    30361 aattaacaaa atttagtaaa attgcatcat ttaccagcgc atttagttgt tttgctataa
    30421 aagaaccatt tggtttttct tctggatgat tttgattggt taaaaaaata ataaaaaggt
    30481 ctatttctgg ataaaaccat aaagaacatc ctgtccatcc agtatgccca aaagaataac
    30541 cagaaggaat acttccttta acaaaagaat taggagcact tatgttccac ccaagtccat
    30601 attcattttt atttagagga gatgcttttg aaatcattgt tttaactatt tcaggattta
    30661 gtacttttac ttcattataa ataccctcat ttaatataaa ttgagcaaat attgataaat
    30721 cacaagcagt agaaaaaaga ccggcatttc ctgctactcc acccataaca tatgcctgca
    30781 tgtcgttgac ttttcccttt aataatttac catctctata aatggtagga gcacatctta
    30841 cccataattt ttcaggtgga ttaaacattg tgtctttcat tcccaatggt tcaaatatct
    30901 tttcttttgt aaatacatct aaagattttc cactaactat ttcaactatt ttccccaata
    30961 taatatatcc taaacaacta taattaaatt cagagtcagg tttataaata ggtgtttcct
    31021 cacatattct ttcaagtatt tttgttggtg tattatgaaa tttttggtta gataaaccag
    31081 aataatgggt aagtaacatt tttattgtaa tttctttttt cccatttttc ccaaattcag
    31141 gaatatactt tataacttta tcatttactg atattttact ttcttcaacc aatatcatta
    31201 ttgatgttgc agtagcaact ggtttagtta tagaggcaag gtcaaaaatt gtattttttc
    31261 ccatttttat tttttccggt gtaatttctc tgtttccgta tgctttccaa taaataatat
    31321 catttttatt tccaactaaa agaactgcac caggtattat ttttttatct attccttctt
    31381 taataatttt atctacaaag taaatttttt ttctcatatt ttattcttta ctttctggat
    31441 ttttcaatta acatacataa atatttataa aagttctcct aagagataat tgcgagtacc
    31501 tattgttatt tttactttaa caactttgcc aattaaattt ttttctcctt taaacataac
    31561 aacctttctt tttatatctc tgccaattaa aaaaccctgt tttttttgac tttctttaag
    31621 acataaaaca tctaatattt ttccaaccag gtccttattt ttcataagag aaatttcatc
    31681 ctggaggtct aaaattattt tatgcctgtt ttctttttca ctttgactta ctgaatcatc
    31741 ccatttactt gcaagtgttt ttggtcttgg tgaatacttg aatacaaaaa ggtcatcaaa
    31801 ttgaatttct ttgataatat tatgtgtttg attaaaatca tcttctgttt ctcctggaaa
    31861 acccacaatt atatcagatg taagagaaat ttcaggacat atttgtctac tcttttcaac
    31921 tatttttaag taatcttcaa ttgtatattt tctattcatt ttttcaagaa ttttatttga
    31981 acctgactgt aatgggaagt gaagatgctt ataaagatta ggtattttaa caaaagaaaa
    32041 taaaagttct tctggaatat ctttgggatg agatgtcaaa aatccaattc tctcaattcc
    32101 tgttatttct gaaacattat aaagtaaatc cacaaaacta tgtccgttat atttatattc
    32161 atttacattt tgccctataa gtattatttc tttgcttcca ttttctacat tatttttaat
    32221 ttcttcaata attttttcaa atggtttatg aacaagtttt cctcttgtaa atggaactac
    32281 gcaataagaa cagaaatttt cacatccctt tgatattgtt ataaaagaag atattttatt
    32341 tttttgttta cttttctcat aaataaaggg tttttcactt tctcccgtaa gaattacttt
    32401 ctctcctttt tctatttctg gtaaaatttc aacaagttgg ttgtaagtat ttggtccaga
    32461 aatgacatca acaaaaggaa tttttttaaa tatttcttca ttataaagat taggagtgca
    32521 accaataaga cagaatatct tatttttact ttttattgag tttaaaaatc caaatgctct
    32581 atcttctgca tgttttcgga cactacaagt attaacaaac acaaaggatg cctcttccat
    32641 tttatttact ttttcaaaac catttttatc aaggagagaa gaaagttttt cactatcata
    32701 aaaattcatc tgacaaccat atgtcttaat ataatatttt ttttccatag ttagttatta
    32761 tattttggtg agagagtaga tacagccagc aataattcca acaagtgtaa aaggagtaat
    32821 ttttgttatt attgataaag aaattgagca tacaacaaga ttaaaattat ttattgaaaa
    32881 agaaagggga gaaaaagaag aacttaaaaa attataaaaa ttggaagaaa ttggaacaaa
    32941 aaataaagta atttctccaa ttgctgttcc tatcagtcca cataaaaaaa t
//
LOCUS       DAOOVW010000018        31012 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_987_length_31012_cov_7.579740, whole genome shotgun sequence.
ACCESSION   DAOOVW010000018 DAOOVW010000000
VERSION     DAOOVW010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 31012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31012
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_987_length_31012_cov_7.579740"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="PLW95_03820"
     CDS             <1..670
                     /locus_tag="PLW95_03820"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV21791.1"
                     /translation="EKGETLGIIGESGSGKTTLGKIIIKLIEPDKGKIIFKNTDITHI
                     KEKGLRSLRKKFQIIFQNPYRSLNPRMTALEIVMEGVAGNKKEKIEKGKYLLDLVGIK
                     NEKFSNFPYQFSGGERQRISIARALSTEPEFIICDEPTSNLDLSIQAQILNLFIDLKE
                     KFNLTYLFISHDLKVIKFISDRIAVMYKGEIVEIGQTEKIIENPTNPYSKTLFDIEKT
                     QFSI"
     gene            complement(705..2600)
                     /locus_tag="PLW95_03825"
     CDS             complement(705..2600)
                     /locus_tag="PLW95_03825"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HOV21792.1"
                     /translation="MDILKNCQWYRLCLTLVNINTSPAMSSHPFVIIQAIIKGVNSLL
                     SGDEPIIFFHIKGKKHTYKMHQFDKLPIEVFFFKKSPEYYNQWKKTFISYLFDPITGR
                     NFDILEIGEIENRSFDNLLLDIGEIHSEGEICLEFLSPFPFKPQKSKQRTYISKEEFI
                     KVYENRFFRLFGKEIHYTSKKDDFTLLPYYWNYTEIRHPSMSQIGHTQYINGCIGKLY
                     IKGVFKDFLPFLILGSELHTGAKISFSQGYYVLHNNSVPFFDSFFPNKKSIIDTIKIT
                     MERYNNAITNLSETEGFSFNEETYAEKIYEEIKENTYTPSPNRSFYIKKKDGSKRLIE
                     QPCFKDLIVQEYLLRTISGVFDRIFEASSIGFRKGKSREIALDMLRSAVSEGYQYAVE
                     ADIEDFFLSIDLEILTALLDFYIPQKDIILKSVLLKSIKNGYIVGERYFPRVKGLAQG
                     SPLSPVLANLYLYSFNEKIQNSNVKMVRYGDDFIILTKTKEDARNILSQAENFLSGPG
                     LRIKKGGKMKKLLVISNHSPETWSEEQKKGWEQIDFIPFPDVNPYLSLDEVSNKVARE
                     IIEKIRCWLSKNPDGKVCLQGDFSLCYIIFKNIQENIFTFPTTTRNVVVDKDGKRTYE
                     FKFGRWR"
     gene            complement(2602..2844)
                     /locus_tag="PLW95_03830"
     CDS             complement(2602..2844)
                     /locus_tag="PLW95_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21793.1"
                     /translation="MFYTGSLRKAYEPALQGRDKISMRGLIRGDTEKSTPTLLYKRRE
                     FLNKSPLLERGVRGDLEIHPNLPLQRKEIQRKNNKE"
     gene            complement(3224..4735)
                     /locus_tag="PLW95_03835"
     CDS             complement(3224..4735)
                     /locus_tag="PLW95_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21794.1"
                     /translation="MGSLVVKSRPLGHYVLSVFSLLFREKYPHDGQICISAPGRAISR
                     TADLVNLLCDNFAPHLLRKEKVNIDKIPSAQDNFKISTIEISLARKSGPEESISQGGI
                     QEDNIFENFLDKVELLKEKQQEFNVCELEFIFSTLIKEGYSFYLSCPDNFQIHLKEGK
                     KQPVDGKDINLGEVKIDQEGNLVYTPVSDALSGKTTAENCPLISSSIESSLMRIGILK
                     SKDYITLANNMCRFDDVIIALDTNMFYKAQVTTSLLDALNDFVRSDFLSTPNWITTVV
                     SAITIGELEHKATRTSFQADEDTTSFRERRWACRGLQEFMEIRSCVDLEGVSMILTGE
                     ISPGLDFSKEETTRDEIIRKMIKNFLNFIGFYKSSYFLTLDKICEMFAKAEGLNAFYI
                     MRESINPNSSYKLSSLDESSDINNISELIYELGVEFPLKIICGNNNEEIIFYVNTDWP
                     EKSLEDWENRCFMLSVKDEKNKLFKILEENGKRIKLGKLLKGWNEVNAERHTI"
     repeat_region   5124..5556
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5124..5154
                     /rpt_unit_seq="agtccccagtcctggggatgtcctttgggac"
     gene            complement(5668..7617)
                     /locus_tag="PLW95_03840"
     CDS             complement(5668..7617)
                     /locus_tag="PLW95_03840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03986 family CRISPR-associated RAMP protein"
                     /protein_id="HOV21795.1"
                     /translation="MSAPYNFIPINKKVVSAPELPDDFNKFHKNRLSGYIDIKIKTIT
                     PLFIRGEGSEFFSINGKPKIPGSSLRGMIRTLIEIVSYSKFSFFDDKRLYYRAVGDPS
                     SLGNTYRSKMIDFSSSNKIGIIISPKMKAGWLVKKNDKYYIRPAKTDSLGRQFYRING
                     NFITDKIFKVGPHVFNVFDYKKIWFEPPANKDYLKKTFPSKKEVQLEYNRVNNISLSS
                     QNSYKEGCLIASGPLGNKKHYQWIINLPDNNEMELEEEVVSNYENDKQRDERINLLEM
                     VKSPKYKEGIPCFYITDNGSSNKIIAFGHTGFFRLPYEKTIKDHIKQENTDKIDFAEA
                     IFGKENAWATRVFFEDANILNENNYLLEETSPQILSEPKPTAFQHYLEQNSSSTPKDL
                     KHWDDDDTDIRGYKLYWHRDNNNNWKGEIEFSKRAFEEFFKERQDIDEILNEETRGDI
                     TRDDKNDDKYKLNKFFDELDNSEFKKCLKEFALGCEKSHYTIITPVKKGIEFQGRIRF
                     ENLTEEELGALLFVLDLPTNCYHKLGMAKPLGLGSVKITPELFLIEREERYSSLFDEK
                     GWKLCEEKEGNIQEYKKKFEDYIKKQIDSSQSSLWEEERLKLLKIMLDWNNTKIPGWL
                     DKTRYMDLEEFKNRTVLEKPNETAP"
     gene            complement(8403..8957)
                     /gene="csx19"
                     /locus_tag="PLW95_03845"
     CDS             complement(8403..8957)
                     /gene="csx19"
                     /locus_tag="PLW95_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Csx19"
                     /protein_id="HOV21796.1"
                     /translation="MDNEIGCIKKIKSIVELPDDKIDILNNIENFIKNNISDGYIVVW
                     LNYAIFIGKIEKNFIFHNGKTPDFSHLLQLRAFNKDKEIFIWRSSSNTYKCRIRIDNE
                     GENTEVIDAQQVIWGTKSKNLQDGFSEIYEDRGIKLIVPFNNLKLNNNRRLMLKTRNY
                     IRHNEIGQAGFFDSRFVDIYPINK"
     gene            complement(8947..10308)
                     /locus_tag="PLW95_03850"
     CDS             complement(8947..10308)
                     /locus_tag="PLW95_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAMP superfamily CRISPR-associated protein"
                     /protein_id="HOV21797.1"
                     /translation="MDKLTGKIIFTGDIITTSPIIIGTGKGELVDSEIMKGYDKNIPY
                     IPASSFIGALRNYFYSEADLKDINKTQLDYFWGSENTNGKFFQSHFIIDDLILKNEPL
                     ITVRDGIKINNKGIAESGKKYEYEILEPGAEFSLYGEITIRDGFYKNLFLQVIKTIIE
                     DLKNEKIYIGALTTKGFGRIKLKDEKIYQFNFPEDTNKWLEFRKSENWQTIEQKNQIN
                     KNWEEIKPLNKKSKKDIIIEATFSLKNSLIIGSYTGDSDKSHLKSKGSPILSGTSIKG
                     TIRNRAVKIIKTLGGDEKFLDELFGWAEEKSDKEPIKSKVIFEESIIENVEEKEQKRI
                     KIDRFTGGVIQHLLFESKPIWHKDERIKIRIKITDYCNWEVGLILLVLKDLWNEDLPI
                     GGEKNIGRGVLRGIEAEINAGDKKITIRQEGENLEVSNEDKEELENYVNAFLKKIGVK
                     NGQ"
     gene            complement(10310..12526)
                     /locus_tag="PLW95_03855"
     CDS             complement(10310..12526)
                     /locus_tag="PLW95_03855"
                     /inference="COORDINATES: protein motif:HMM:NF015728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAMP superfamily CRISPR-associated protein"
                     /protein_id="HOV21798.1"
                     /translation="MKLKIKTLSYTLVGSGEASVLIDSDIIFDDFGIPFIPAKRIKGL
                     LKESATEIIEILGISNSNYLESIFGTGSNEGKTKFSNLFIKDYKNITELIKEARKDKN
                     SRFVLSKENIIKYFTETRQQTAINEKGVAKPHSLRTYRVLKPFIEFEGTISDKDITDD
                     EKSLLFLAVINLKRIGVRRNRGLGKIKCEIEGISIVDIDKEIKNLTSIPSQQNNTKEV
                     SNELYSCQGKTGEIKKLPFTIKTLSPIIITKQTGDQNTVSTEEYIPGTLIRGILANKF
                     IKKFNLGNDAHKDKVFRELFLDGKLTITPAYPSKEEKEFYPAPLSIHIKKAETLPTEL
                     PSENNIYNLFEEEPGEKTRPLGGFVDYNGNNIVKCAINKRFYFHNTRENRTAGHSVEG
                     GIFYYESIDEGQEFKGYLIGDVSYLSFIKNCFEENKDCFGEKFTENIGRSKTAQYGEI
                     EFSFGEPEEIEKINLTDTEFTLTAISPIILYNEYGFPEVSIERLKKYLEQYFSGGEIE
                     IEEKAFANIDFSEGFMGIWKCKTQREISFKEGTSFKIKLKNSPELKKLSELERDGIGE
                     KRELGFGRVKINWLTDKSQYKGVKYEEQLKKTGNTSATFNKILTSILMDILIENIKHE
                     AIKEANTYQKKITNSLIGRIEKIVMDAKEIKDIEDKLKEMKDKPAGKELQKIKIWDNL
                     KNIDIIEPIIQNFRTPYSSFIKGFPFDLNNNEKFELFKIYWLTFFRMARYLNKKGK"
     gene            complement(12534..14051)
                     /locus_tag="PLW95_03860"
     CDS             complement(12534..14051)
                     /locus_tag="PLW95_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21799.1"
                     /translation="MVGVLIDTVSIQNYIFSSNKLIENVGASYLVEQIYEEYLKDALR
                     TFHSTSPNLDTWKNNPSYMNFSPYGIGYIGGGNALILFEVNNGKHKEFIKNFTNILLE
                     KTPGLRTSFGINPNFNLNNSANSFTNSMKEIHKNLKKNKNIYFPIITLPKYGITADCP
                     FSGESAEIYYKDKWISNVSKVKIEAGEKSLSHIQDMLKDELGETYTFTNEIDKLGQVE
                     HKNYIAVVHIDGNNMGKRFEKCNTLKELRNLSIRVKENTQKAFKETIEDLVGLIENRI
                     LGPKNNFHFHTDGNGKTILPIRPIIIGGDDITFVCDGRLGVYLAEKFITKFSEQQIKG
                     EKLSACAGVSIVKTKYPFYRAYQLSEQLCENAKKESRKEENSSYLDFLISSRGFSGTL
                     EEIRERSFKIPYGLLPFGPYRIDNNTQENSIYHLKGGIKFFNNWTRTKIEQLREVTTK
                     SESEIKMFVKEMDIKGYTFYEIPYAPPYSEDIRVNDKTPYFDIIELMDFYPKELL"
     gene            complement(14053..15336)
                     /locus_tag="PLW95_03865"
     CDS             complement(14053..15336)
                     /locus_tag="PLW95_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1887 domain-containing protein"
                     /protein_id="HOV21800.1"
                     /translation="MIVLIIGTNPLPNYVVADYLKDKDRQFCLVYSEERCEINQSGTY
                     KFAESIKEQLIKNGCREEQFQFLPLSNVSLVEDIRIDLQKLFSSQNFERIHLNYTGGT
                     KVMAACLHEFIKEKFPYADFSYLDARTYKLLYDDRKIEPETGDMRTYVSLDISTLLSL
                     HGYNIKEPVRYFMEKDFGKVLNILINLLNTGEINEFIEWKDEYLRKIFYDEDGNLIDT
                     IKKFKENIEKESLSKIVGNFPTSLTPNIKEILNSFPDEKKIITNENTLWTPQPSDINK
                     ALENKTSVVDFLDGKWLEWYILDKVKNIEGITGYGCGLQANKPNTRNFELDVFLIKGY
                     QLIGISVTTAGFRNKKIEKYLCKSKGFEVIHRTKQIGGDEAKSVLITGLDSNSALELE
                     KDIQGITGSAEEHFKVFGKDDIKDIETKIKDFVGG"
     gene            complement(15371..16105)
                     /locus_tag="PLW95_03870"
     CDS             complement(15371..16105)
                     /locus_tag="PLW95_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21801.1"
                     /translation="MKIKKYKFLIFFSLIGLNIFSTCPPAYSSEIVFVWIDVTNSYIG
                     YDNLGKLINYKEWAVKDLSSGIARIISGKNFRLYWKNYYFVIGKITGRQKGYDKLYEF
                     PLKGNKWSEIMQILNNLENIPIEKYSENMTDIYGTYEYMARYLSKFKKPPSVRAIYYS
                     DMIHELNRPKPNPESLNVLKGAKITAIFCQGEEYNEKLIAENFNRFQEQVNFLKDIGV
                     DFFYYEPPGVSYQIYGETYLEKALKR"
     gene            complement(16086..17219)
                     /locus_tag="PLW95_03875"
     CDS             complement(16086..17219)
                     /locus_tag="PLW95_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21802.1"
                     /translation="MIKWFSHLSVVWQWIIGWTIINFVVSLLQKFGEEFGKLDPVSKI
                     TETLIGEGKKPWFITAPFLIIYHIFKSIYYIAWLVILFLLLLISPFVIIIWECIRYFF
                     ALLLFIGRVLREYLFRPEVLIQIAGIIINFVVVYWAVSGMQGGGTNSIIRSTGIVLTE
                     NIGFIVFLMVLSNNLLDNILKLRIPDQNKTISPDEKRASLKNLILGLILVPIISYGLF
                     KIFLVDYNYNVEAVKVERELLVRGLFGPASKTFSLESLTSNIPPEAMGWVTVVIPVIC
                     GIFSFQFYKKPFVMPEWKVPLPYGRTLCGTMLKWFSPVGKYLLKSIKMILFYIIVIAC
                     WCYFLIDPIFQFYKNRKEKNIVYENNKIIDVEIVEKKDENKKI"
     gene            complement(17655..18806)
                     /locus_tag="PLW95_03880"
     CDS             complement(17655..18806)
                     /locus_tag="PLW95_03880"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF003705.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOV21803.1"
                     /translation="MKEKIKRKFIEIFGDGDIFNIKSPGRVNLIGEHTDYQGGYVLPI
                     AINKYIYFCGRKRKDKKIKIYSFNYDDYLEVDILDIKFNKEKKWANYIFGVIKEFEKS
                     KIDISGFEVAYGGNIPVGSGLSSSASVEIGTISILMKTFNIKIPDIQVINIARCAENN
                     FVGVNCGIMDQFIIYLGKKDNAIFLNCNNLNYSYVPFKTGDYQLLLVDTKKERTLSNS
                     VYNERVKEVGEVVNIIKKFVDIDNLGQVDFKILEKYKSLIGQTEYRRGKHIVEENERV
                     KKSVELLKNGEIEKFGELLYSSHKSLKELYNVSCNELDFIVDFSKNFEGVAGSRLTGA
                     GLGGCCIVLIKKIIIEEFKEKLESAYFEKFKIKPAFYDVLAVDGAKSSI"
     gene            18917..19552
                     /locus_tag="PLW95_03885"
     CDS             18917..19552
                     /locus_tag="PLW95_03885"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOV21804.1"
                     /translation="MEKKIEKQLSLPFKHAFGMSIRNIKIRFGRAIIITMSILLGIAF
                     LMSILTGNALTMSLIQNGPEEIRILLQKNIEEVKIRQVWLVSLSLLVCVVGIINSMLM
                     SVTERSKEIGTMKCLGALDRFVMELFLLESSIHGILGSLLGSIVGIVVMTLVYLIQYK
                     GLILKLFPFAVIGKYLLFSVILGTFLAVIGALYPAYVSAKMEPAEAMRREV"
     gene            19566..20303
                     /locus_tag="PLW95_03890"
     CDS             19566..20303
                     /locus_tag="PLW95_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV21805.1"
                     /translation="MPKETIVRTIGLKKNYMLGRVKLEVLKGIDIEILKGEYISIMGP
                     SGSGKTTLFNMIGGLDKPTEGKVYIDEVDVAQLDAYELAWLRCRKIGYIFQTFNLIPV
                     MSALENVLLPMIFAGMTTDDAREKAKNLLETVGLGDRLHHKPFELSGGQQQRVAVARA
                     LANDPAIVLADEPTGNLDLKTGKEIINLLREMNREKQVSIITATHDLKMLDVSDRVIW
                     LRDGIIERVEDRENINIKVGEVEGEEI"
     gene            20306..22000
                     /locus_tag="PLW95_03895"
     CDS             20306..22000
                     /locus_tag="PLW95_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21806.1"
                     /translation="MKTIVLVDSSKVVQYKRVGKTILNCLEHFGIWYEIIDLNWAGIS
                     YEEIHNNVSVIILGQEGISSSISDTDFSLILKSVENGTGLVIFDGYLNKYSEKFLKTI
                     GIENFSSKKTQTVSLEKNWITEISPDVVYLKRDTLHYCPSVKSDWDIFMKNEQGNGCG
                     FFRKYGKGKIIFWTTLPSIFLDEYLGHTEGLDGVFFRSIIWTSKKPFITKTMPPFVTG
                     RIDDASGSGSPVSKYPETVNGLKYLDILNKYKFIPNIGLFIDDLKEEDDKKIREKHYD
                     GLAEFSPHAFSDPENKNEFPIYLKHSGEEFSDSELKENFEKVDIKFGKIGINISKTLN
                     AHFGEVGLKTFPFLKERGVKYFMNSIRVGKRWDDPLSKIWEIKPYNKPFFSLGYIPED
                     EYFFNVEASPPKIDISASALDSESPDFDFLYGCTTFWNENKKVEVEKAAKRGIFKIQR
                     GLENGFFGSLSTHEQRISNVPILQFENIISSISNEINKMSVIFKSYDFISQYAESRAN
                     YTIDKIEITNKISIVLKGENKVEQLLYLFTETNGLPKTSFLKVPTFQKQIILNFSI"
     gene            22022..23002
                     /locus_tag="PLW95_03900"
     CDS             22022..23002
                     /locus_tag="PLW95_03900"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOV21807.1"
                     /translation="MGKLKIGFIGCGSISYGHLYQIKKIEDVEITGVFDPKKENVNKF
                     LSEAGEGKIFTSEEELIKNVDAVIICSPHTSHFPQIKTALENGVDVLVEKPAVVNYEE
                     AIKLRKLIEKTGRKVVVGYQKHYMELPLSVKKIIQEGKIGKILFLSGFLSQNWIEGIT
                     KSGRIWRFDPKFSGKGQLTDSGSHFISMLFWITSLTPEKVGAFIEFFDKKVDINSSFT
                     VKFKEGALGSFSILGIDPSYREALMIWGEKGLIKLSLFGENSYIHYNGEKEPKKISVS
                     KPTIISPVDDLIKCIRENKEPETDISVIEKVALLSDKIYESFNKEKIVNC"
     gene            23030..23821
                     /locus_tag="PLW95_03905"
     CDS             23030..23821
                     /locus_tag="PLW95_03905"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005896374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOV21808.1"
                     /translation="MDILKINCEKVCDYLVSFLKEEVSKIGFNSVIFGLSGGLDSSLV
                     AFLCKMAFGRNNTYGIILPYKDTPKESVDHAYLIGRTLGIRHLRFNITPQIDYYYKNF
                     PQATKLRKGNKMARERMSILYDLSSHFKSLVVGTSNKSEIYLGYGTLHGDTAYDINPV
                     ADLYKTQIYQLSEYLKIPEEIRIKKPSAELWEGQTDEEELGFSYEVADKVLFYSFEKN
                     MKQQDIMKEIKIDERTFLKIIERVKKYEFKRKPPLMAKIPDEIKY"
     gene            complement(23818..24420)
                     /locus_tag="PLW95_03910"
     CDS             complement(23818..24420)
                     /locus_tag="PLW95_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21809.1"
                     /translation="MKIENINGNLNSVNDGYITFLFYDGKGKVNYEIGEKLNFDFSIK
                     EMNLEKLILSLTKEIHISGIVNVDGNFEKEDENYIIESEILSVRKKGIKQIMNFGAVN
                     FFATLAGGNPIKTIGSSNFYYGQMGMKITLKNNFLTLEGLLGEKENKQYLIKGVHLRN
                     SINLTIDKNTNTIEISDLKERIQTAIERTKSGSNITSNFY"
     gene            complement(24522..25415)
                     /locus_tag="PLW95_03915"
     CDS             complement(24522..25415)
                     /locus_tag="PLW95_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21810.1"
                     /translation="MKKRKKIVISIVVFLVFFVFISLKIAERIINKKLSSLSNIPLKI
                     ENIDLSFSGAKIKNLSYKQNAFFILLNELKIKPTFTKVSFAGPGEVGNQQQERNITIN
                     GTISGNIKSGNVNIENTNIKIEEIGDIQVQGNLKNWGKTDQDVIVKCNKIDIKQVCSF
                     FNLKVNLEGNVSGNIMLYKKDEKQRIDFDIKVEELKVEKGEGIIVLLKGCFYPTEKKG
                     EIERGVAFIDSNELRFWGEIDNENFNLSFTGEKIQIEKLLRILPEDIREKYKIDISGG
                     TANFNHFSLSKVKKNFKQLVT"
     gene            complement(25412..26458)
                     /locus_tag="PLW95_03920"
     CDS             complement(25412..26458)
                     /locus_tag="PLW95_03920"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HOV21811.1"
                     /translation="MPNKFDSPVVIDNFIEKWAGKIKPSLEFKGKTKEDVFIWKEKLI
                     KKIKKLLGEFPTPSDLNPELLSVEEKEKFIREKWIIQSEKDCFVPLYLLIPKGNKKKY
                     PAILCCHGHGSYGKDPVAGVHNNNPDIINNIKMHNYNYGQQMAENGFITICPDWRGFG
                     ERGGYQDTFFPGRDKCNVYFLQHLILGRTLLGANIFDGMRVIDFLLTREEVDKERIGC
                     MGLSFGGTMATYLTLLDERIKTGDIICYATTTLHYAISRPNFCGSQIVPFLYKYADVG
                     DVIGCICPKPLLIESGVNDTCFWIDSAKKAHEKIKNVYKVAGVENNLWIEIFPGEHSF
                     SGRKAFEFFGKYLK"
     gene            complement(26645..27559)
                     /locus_tag="PLW95_03925"
     CDS             complement(26645..27559)
                     /locus_tag="PLW95_03925"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOV21812.1"
                     /translation="MRICIIGGTGHIGINLVKFLLRENYEIFVVARGIKQTPENFRIK
                     FVKKNYDNNKIEWENLFREIKPKIVVDILGSYAPLVYETSKIYCKHFILCGSVWMFGE
                     SKIVPTPEQTQSPCIFSNYAKRYAEMLELKNTAKKEGIIFTAIMPPNICGPGKIPLDC
                     YGDRNIENHQNHKKGYPVPLPEPGQTLIGPCDAEDVAKAFFLAVLKSQKAADEIFNVG
                     SAYAITSLNFVQTYEKIYGVKIPIQWYSWQEYCTKINPDPGANFHFKAHMCPDIRKIS
                     QKLGYKPSYTPEETMERAVLWMKQQVLI"
     gene            27911..29077
                     /locus_tag="PLW95_03930"
     CDS             27911..29077
                     /locus_tag="PLW95_03930"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOV21813.1"
                     /translation="MIKKFKFICEPQVFYGNNSVSLLPSIVKETKRKKLLVVVDSNFS
                     KTSFFRDIEKKLSGEKIDFVVFDKISGEPTTDIGDECAEFGKENKCDIVCGIGGGSSL
                     DVAKASSVIITNGGCVKDYQGLNKVPGPGLPKIMIPTTAGTGSEVTFTAVFIREDTKE
                     KGGINSPYLYPEYAILDPVLTVSLPTNITSSTGLDALCHAIESYISKNSNFLSESISL
                     CAIKYIWENLPIVYSKDEDLKAREKMLYGSFLAGLGLVNAGVTAVHSISYPLGGVYRV
                     PHGVGNGLLLPYVLEFDIKKDISYTVERKLSSIVDIVRSDFKGDEKEKAKFLVDEIKN
                     FLKLFKMPKLRDFNIDKENFSKLAEDALKVSVPIMNNPVAIKKEDIIEIYNNAY"
     gene            complement(29074..29523)
                     /locus_tag="PLW95_03935"
     CDS             complement(29074..29523)
                     /locus_tag="PLW95_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21814.1"
                     /translation="MGKIKKFFVYFLILICIFLLLKKFIFISEEDRIKQTIKIAEKAI
                     ENNNYTEFMKHFSFEYRDEYGNTWGTLFFLVKNILKNYQKVDISIRNLNIEIFDKTAV
                     VKIFGEGKAISPSGEIIKEAGRFLIKFKQEGKKWKIVWVEEDKYRFD"
     gene            complement(29511..30008)
                     /gene="lepB"
                     /locus_tag="PLW95_03940"
     CDS             complement(29511..30008)
                     /gene="lepB"
                     /locus_tag="PLW95_03940"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOV21815.1"
                     /translation="MDKIVEMGIPSLKRIFFWICLILVLFLTRAFLIQPFKTFGPSMK
                     PTISDNKIIFVNKIIYKFREPERGEIVVFRTSDRPYVYFIKRVIGKEGERVEIINGDV
                     IINGKKLVEPYVINKNDNWNMPEIKIKKGYIFVIGDNRDMDISQHLLVDVSIKNIIGK
                     VIWVK"
     gene            complement(29972..30877)
                     /locus_tag="PLW95_03945"
     CDS             complement(29972..30877)
                     /locus_tag="PLW95_03945"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOV21816.1"
                     /translation="MRKFLFIFIILINSILSSISVYFTPSKQADEEILKVIKGAKNSI
                     FIASYNFNYKEIIEILKNKKLDELKIFIGKESNLPFSQSCIQVYKGSNLFHSKFIIID
                     NEITIIGSGNFTKNDFHLFHNNFLIIKDKNISEYFKENFFSLWNNKENNKSPFLSETL
                     EIYFSPEENLEEKIIEEIEKAKSSIYFSHFYFSSENIARHLIDKKKKGVDINGIFERY
                     NTSNYSTFYILNDYGINVRKNNMAGFLHDKFFVIDEKVVITGSYNPTVSARKNYETIL
                     ILRNKEIAGKYLKEWIKLWRWESLP"
BASE COUNT        10050 a         5059 c         4338 g        11565 t
ORIGIN      
        1 agaaaaaggg gaaacacttg gtataattgg tgaaagtgga agtggtaaaa caacacttgg
       61 caaaataatt ataaaattga tagaaccaga taaaggaaaa ataattttta aaaacacaga
      121 tataactcat ataaaagaaa agggattaag aagtttaaga aaaaaattcc agataatatt
      181 ccaaaatcct tataggtcat taaatccaag aatgactgcc cttgaaattg ttatggaagg
      241 tgttgctgga aataaaaaag agaaaataga aaaaggcaaa tatcttttag accttgttgg
      301 aataaaaaat gagaaattta gtaatttccc ttaccaattt tcaggtggag aaagacagag
      361 aatttcaata gcaagggcat tatcaacaga accagaattt attatatgtg atgaaccaac
      421 ttcaaatctt gacttatcaa ttcaggcaca aattttaaac ttatttattg acctaaaaga
      481 aaaattcaat ctcacatacc tttttatttc tcatgacctt aaagttataa aatttatttc
      541 tgaccgaatt gctgttatgt ataaaggaga gattgttgaa attggacaga cagaaaaaat
      601 tatagaaaac ccgacaaatc catattcaaa aaccctcttt gatattgaaa aaacccaatt
      661 ctctatttaa ttttttttac actatattaa ctttcccaca aaatttacct ccaacgccca
      721 aatttgaatt catatgttct tttcccatcc ttatctacta cgacatttct tgtcgttgtt
      781 ggaaaggtaa atatattctc ttgaatattt ttaaagataa tataacataa agaaaaatct
      841 ccttgaagac atactttccc atcaggattt tttgataacc agcatctgat tttttcaata
      901 atttctcttg caactttatt agatacttca tcaagagaaa ggtaagggtt cacatcagga
      961 aatgggatga aatcaatctg ctcccatccc tttttttgtt cttctgacca agtttcagga
     1021 ctatggtttg aaattactaa taattttttc atcttacccc cttttttaat tcttaatccc
     1081 ggtcctgaga gaaaattctc tgcctgggag agaatatttc gtgcatcctc ttttgttttg
     1141 gtaagaataa taaagtcatc tccatacctt accattttta cattagaatt ttgtattttt
     1201 tcattaaaag aatacagata cagattagcc agaacaggag aaagtggact tccctgagcc
     1261 agccctttaa ctcgcgggaa atatctttca ccaacgatat acccattttt tatggacttt
     1321 aacagaacac ttttcaaaat tatatctttt tgtggaatgt aaaaatcaag cagagcagtt
     1381 aaaatttcaa ggtcaataga aaggaaaaaa tcttcaatat ctgcttcaac agcatactga
     1441 taaccttctg aaactgctga tcttagcata tccagagcaa tctctcttga ttttcccttt
     1501 ctgaaaccaa ttgaacttgc ttcaaatatt ctatcaaaaa cgcctgatat agtccttaaa
     1561 agatactcct ggacaatcag gtctttaaaa caaggttgtt ctatcaatct tttagaacca
     1621 tccttctttt taatatagaa ggacctattg ggtgaaggag tataagtatt ttcttttatt
     1681 tcttcatata tcttttctgc atatgtttct tcattaaaag aaaacccttc tgtttctgac
     1741 aaatttgtaa ttgcattatt atacctttcc atagttattt taattgtatc aattattgac
     1801 ttcttatttg gaaaaaacga atcaaaaaaa ggaacagagt tattgtgtaa tacataataa
     1861 ccctgtgaaa atgaaatctt tgctcctgta tgaagttcac taccaagaat taaaaaagga
     1921 aggaagtcct taaatactcc ctttatgtat aatttcccaa tgcagccgtt tatatattga
     1981 gtatgaccaa tctgggacat ggaaggatgt ctaatttcag tatagttcca ataataaggc
     2041 aataatgtaa aatcatcttt tttgctggta tagtgtattt ccttgccaaa caacctaaaa
     2101 aacctatttt catatacctt gataaattct tctttggaaa tatatgttct ttgtttgctt
     2161 ttttgtggct taaaaggaaa tggtgatagg aattccaaac atatttcacc ttctgaatgt
     2221 atttctccaa tatccaaaag aagattatca aaagacctgt tttcaatttc accaatttca
     2281 agaatatcaa aatttctacc tgttattggg tcaaaaagat aagaaataaa tgtttttttc
     2341 cattgattat aatattcagg ggactttttg aaaaaaaaca cctcaatagg tagtttatca
     2401 aactggtgca tcttatatgt gtgttttttc cctttaatat ggaaaaaaat aataggttca
     2461 tcacctgata ataatgaatt aacccctttt attattgcct ggattataac aaagggatga
     2521 gaagacattg caggcgatgt atttatgttc acaagagtaa gacataatct ataccactgg
     2581 caatttttca atatatccat cttattcctt attatttttc ctttgtattt ccttcctttg
     2641 taaaggaagg ttaggatgga tttctaaatc tcccctaacc cctctttcta aaagagggga
     2701 tttattaaga aactccctcc ttttgtaaag gagggttggg gtggatttct ctgtatctcc
     2761 tcttattaaa ccccgcatgc ttatcttatc tctaccttgt agggctggtt cgtatgcttt
     2821 ccttaaactg cctgtgtaga acaatccccc tcgccccctt gtgggagagg gccaggggag
     2881 atttctcaag tccccagtcc tggggatgtc ctttgggaca gtagaacttt gcccaaaaaa
     2941 ggaggattaa gtttcagtcc ccagtaccag ggatgtcctt tgggacagtg tcagttcaac
     3001 aagatggtag cgtaaaaatg tttcagtccc cagtcctggg gatgtccttt gggacgctga
     3061 ataaagtttt caacgagcag ttttattagg tttttccaat acattccgat tcttaaattc
     3121 taatttgtcc ctttacataa ctggtaaatt tgagcaagtt cagaagaata ttcaatcccc
     3181 ttatcatctt atcttctttt attaccttct ttaaaatctt ttttcaaata gtatgtcttt
     3241 ctgcatttac ttcattccac cctttaagta attttcctaa ctttattctt tttccatttt
     3301 cttccaggat tttaaaaagt ttgttctttt cgtccttaac tgaaagcata aaacagcgat
     3361 tttcccaatc ttcaagggat ttttctggcc aatctgtatt tacataaaaa ataatttctt
     3421 cattattatt tccacagatt atttttaatg ggaattcaac acctaattca tagattaact
     3481 cagagatgtt atttatatca cttgattcat caagagaact caatttataa gaggaatttg
     3541 ggttaatact ttctctcatg atgtaaaatg catttaatcc ctctgctttt gcaaacattt
     3601 cacaaatctt atccaaggtc aaaaaatatg aacttttata aaaacctata aaattaagaa
     3661 aattttttat catctttctt attatctcat cgcgagttgt ctcctccttt gagaaatcaa
     3721 gtccaggcga tatttctcct gttaatatca tggaaactcc ctctaaatct acacaacttc
     3781 ttatttccat aaattcctga agtccacggc aggcccagcg tctttcccga aatgatgttg
     3841 tatcttcgtc cgcttggaaa gatgttcttg ttgctttatg ttctaactct ccaatagtta
     3901 ttgcgcttac tactgtggtt atccaatttg gagtagagag gaaatcagaa cgtacaaaat
     3961 catttaatgc atctaataag ctagtcgtta cttgtgcctt ataaaacata tttgtatcaa
     4021 gagcaataat aacatcatca aatcggcaca tattattggc aagtgtaatg taatctttgg
     4081 actttagaat tcctattctc atcaaggatg attctataga ggaacttatt aaaggacaat
     4141 tctctgctgt tgtcttacct gataaagcgt cagaaacagg ggtataaaca agattaccct
     4201 cctggtctat cttaacttct ccgaggttta tatctttacc atccacgggc tgctttttcc
     4261 cttcttttaa atgaatctgg aaattatcag ggcacgaaag ataaaaagaa tatccttcct
     4321 taattaaagt tgagaatata aattctaatt cacaaacatt aaactcttgc tgtttttctt
     4381 ttaaaagttc taccttatct aaaaagttct caaaaatatt atcttcttga attcctccct
     4441 gagaaatgga ctcttccgga ccagatttcc gagcaagaga aatttcaatt gtggatattt
     4501 taaaattatc ttgtgctgaa ggtattttat caatattcac tttttctttc cttaataaat
     4561 gaggtgcgaa gttatcgcaa agtaagttta ctaaatctgc ggttctactg attgctctgc
     4621 cgggagcact aatgcaaatt tgtccatcat gaggatattt ttctctaaat aataaagaga
     4681 atacagaaag aacatagtgt ccgagtggcc tactttttac cactaaactt cccatttttt
     4741 acctcctttc aattttaact tattattcta aacaacaaag gacaatttct ttatcattgg
     4801 gcctattaaa cacactcttc tgcatccttg tcccgtaatc cttcatcctt ttggtcattt
     4861 tgtttcttca gtattgtcaa aaaatttatt ctctttttca cttatccctc accaaatccc
     4921 ctctacctcc cccttatcaa ggggagaacc taaatctcct ctaacccctc tttctaaaag
     4981 aggggaattc tcttttaatt ccttcctttt gtaaaggaag gttaggatgg attgcttctc
     5041 cctccttttc caaaggaggg ttggggtgga ttttctatct ttttcctctc cctttgtggg
     5101 agagggccag gggagatttc tcaagtcccc agtcctgggg atgtcctttg ggacgattta
     5161 aagattatct taggagcagt aatgtttcag tccccagtcc tggggatgtc ctttgggact
     5221 tagctggtca agctggcaaa attatggagt ttcagtcccc agtcctgggg atgtcctttg
     5281 ggactattta tttagcaaaa ggatcagcgt cttgggtgtt tcagtccctg gttccaggga
     5341 tgtcctttgg gacttttaga aaacggaaaa acgagagtta tttttgtttc agtccccaat
     5401 tctggggatg tcctttggga caggaagccc cctctattca atcccgattt gtttcagtcc
     5461 ccaattctgg ggatgtcctt tgggacagta tggccgcatc cttgggggtt gggagtaatc
     5521 gtttcagtcc ccagtcctgg ggatgtcctt tgggactctt cctttgaaaa ccattgatat
     5581 tcaaaggatg tataagcaaa aattcctaac ctcccacaaa attgtacccc ttttcccatt
     5641 ttcccccata ggttctgaat aattttttca aggagcagtt tcattaggtt tttccaatac
     5701 agtccgattc ttaaattctt ctaaatccat ataccttgtt ttatcaagcc agccaggtat
     5761 ttttgtatta ttccagtcaa gcattatttt taaaagtttt aacctctctt cttcccacag
     5821 agaactttga cttgaatcaa tttgtttctt tatataatct tcaaatttct ttttatactc
     5881 ttgtatatta ccttcttttt cttcgcataa tttccatccc ttctcatcaa aaagagaact
     5941 atacctttct tctctctcta ttaaaaaaag ttcaggtgtg attttaactg aaccaagtcc
     6001 cagtggtttt gccatcccta atttatgata gcagttggta ggtaggtcaa gaacaaaaag
     6061 aagtgcaccc aactcttctt ctgtaaggtt ttcaaacctt atcctccctt gaaattcaat
     6121 accctttttg acaggagtta ttatagtgta gtggcttttt tcgcatccta aggcaaattc
     6181 cttcaggcat ttcttgaatt cagagttatc aagttcatca aaaaatttat tgagtttgta
     6241 cttatcatca tttttatcat ctcttgttat atcacccctt gtttcttcat taagtatttc
     6301 atctatatcc tgtctctcct tgaaaaattc ttcaaaggcc cttttgctaa actcaatttc
     6361 tcctttccag ttattattgt tgtctctgtg ccagtaaagt ttgtatcccc ttatatctgt
     6421 atcatcatcg tcccagtgtt ttaagtcttt gggagtagat gaactattct gttctaaata
     6481 gtgttgaaag gcagttggtt ttggctctga caaaatctgg ggagatgtct cttcaagcag
     6541 gtaattattt tcatttaaga tattggcatc ttcaaaaaat acccttgttg cccaggcatt
     6601 ttctttccca aatattgcct ctgcaaagtc aattttgtct gtattttcct gttttatatg
     6661 gtcttttatt gttttttcat atggcagacg gaaaaaaccc gtatgcccaa atgctattat
     6721 tttattactg gacccattat cagtaatata aaaacaaggt atcccctctt tgtatttagg
     6781 actctttacc atttcaagaa gatttatcct ttcatctcgt tgtttatcat tctcgtaatt
     6841 tgatacaacc tcttcctcta attccatctc gttgttatca ggtaaattta taatccattg
     6901 ataatgtttt ttattcccta atggaccaga agcaatgagg catccttctt tataggaatt
     6961 ttgagacgat aaggaaatat tattcactct attatattct aattgtactt cttttttact
     7021 aggaaatgtt tttttaagat agtccttgtt agcaggtggt tcaaaccaga tttttttgta
     7081 gtcaaataca ttaaacacat ggggacctac tttaaaaatt ttgtctgtga taaaatttcc
     7141 atttattcta taaaactgcc taccaagaga atcggttttt gctggtcgta tataatattt
     7201 gtcatttttt ttgactaacc aacctgcttt cattttagga cttattatta ttcctatttt
     7261 attagaagat gaaaaatcaa tcattttgct ccgatatgta tttcctaaac tacttggatc
     7321 tccaactgca cggtaatata atcttttgtc atcaaaaaag gaaaatttgc tgtaagagac
     7381 aatttcaatt agcgttctta tcattcctct taaagaactt cccggaattt tgggtttgcc
     7441 attaattgaa aaaaattcag agccctcacc tcttataaat agtggtgtta ttgttttaat
     7501 ttttatgtct atgtatcctg ataatctgtt cttatgaaat ttattaaaat catcaggtaa
     7561 ttctggtgca gaaacaacct ttttgttaat aggaatgaaa ttgtatggag cactcattgt
     7621 tttcctcctt gttatctggt agttcaaaat atttttttgt atttcttcct ttatatttcc
     7681 tccccttttc caaagggtca gggggagatt tctctaaaat cccctctaac cacccccacc
     7741 tatatcctcc cccctcatat ggggaggaat taaatcccct ctaaccccct ttgtaaaagg
     7801 ggggaatggg gggatttata aaaggagggt tggggtggat ttttctatct ttttcccctc
     7861 gcccccttac gggagagggc caaagggaga tttctcaagt ccccagtcct ggggatgtcc
     7921 tttgggacta atcaaattgt ctggatatgg attttttatt gtttcagtcc ccagttctgg
     7981 ggatgtcctt tgggacgaaa aacttgatgg agtataaatt taaagtgttt cagtccccag
     8041 gactggcgat gtcctttggg acaaggatgt tcctggagat tgggtcaaag tatattagtt
     8101 tcagtcccca gttctgggga tgtcctttgg gacaaaaagt tccttataat acaaaagttg
     8161 tagcgtttca gtccccagtc ctggggatgt cctttgggac atgtttccga ggaaacacga
     8221 aaaatgattc aagtttcagt ccccagacct ggggatgtcc tttaggactc ttcctttgaa
     8281 aaccattgat attcaaagga tgtattgaca aaaatttcta atctcccaca aaattgtccc
     8341 tttttcccat tttccccata ggttctgaat aaagttttca aagagcaatt ttattatatg
     8401 tttcacttat taatagggta gatatctaca aatctgctgt caaaaaatcc tgcctgtcct
     8461 atttcattat gtcttatata gtttcttgtt ttaagcataa gacgtctatt attattcaat
     8521 ttaaggttat taaaaggaac aataagtttt atacctctat cttcataaat ctcactaaag
     8581 ccatcctgta aattttttga ctttgttccc catattactt gttgtgcatc aattacttct
     8641 gtattttcac cttcattatc tattcttatt ctgcatttat aagtattaga ggatgacctc
     8701 catatgaata tttctttatc tttattgaag gcacgcaatt gaagaagatg cgaaaagtca
     8761 ggtgtctttc cattatgaaa aataaaattt ttttcaattt tcccaataaa aatagcataa
     8821 tttagccaga ccacaatata tccatcagaa atgttatttt taataaaatt ctctatatta
     8881 ttaaggatat ctatcttatc atcaggtagt tctactatac ttttaatttt ttttatacaa
     8941 cctatttcat tgtccatttt taactccaat tttttttaaa aaggcattta cataattttc
     9001 aagttcttct ttatcttcat tagatacttc aaggttttca ccctcctgtc ttattgtaat
     9061 ttttttatct cctgcgttaa tctctgcctc aatacccctt aaaacacctc tgccgatatt
     9121 tttttctcca ccaataggta ggtcctcatt ccacaggtct tttaatacta aaagaataag
     9181 tcctacctcc caattgcagt agtcagttat ttttattctt attttaattc tttcatcttt
     9241 atgccatatt ggcttgcttt caaataatag atgctgaata actccacctg tgaacctatc
     9301 aatttttatc cttttctgct ctttttcttc tacattttca ataatacttt cctcaaatat
     9361 taccttactt tttattggtt ctttatcaga tttttcttct gcccagccaa atagttcatc
     9421 taaaaatttt tcatcaccac caagtgtttt tataattttg actgccctat ttcttatagt
     9481 tcccttgatt gaagtgccgg aaaggatagg acttcctttt gatttaagat gcgatttatc
     9541 agaatctcca gtatatgaac caataatcag agaatttttt aaagaaaatg ttgcttcaat
     9601 aataatgtct ttttttgatt ttttatttaa tggtttgatt tcttcccaat ttttattaat
     9661 ttgatttttt tgttctattg tttgccaatt ttctgacttt ctaaattcca accatttatt
     9721 tgtatcttct gggaaattaa attgatatat tttttcatct ttaagtttta ttcttccaaa
     9781 tccctttgta gttaaagcac caatataaat tttttcattt ttcaggtctt ctataattgt
     9841 ctttatgact tgaagaaaga gatttttgta aaacccatct cttattgtta tttctccata
     9901 aagtgaaaat tctgcacctg gttccaatat ctcatattca tattttttgc ctgattctgc
     9961 aatccctttg ttgttaattt ttattccatc tcttacagta ataagaggtt cattttttaa
    10021 tataaggtca tcaataataa aatggctctg aaaaaatttg ccgtttgtat tttcacttcc
    10081 ccagaagtaa tcaagttgtg ttttattaat gtcttttaaa tcggcttcac tataaaaata
    10141 gtttcttaat gcaccaataa atgaacttgc aggaatatag ggaatgtttt tgtcataacc
    10201 tttcataatt tcgctatcaa ccaattctcc tttacctgtt cctataataa ttggtgaggt
    10261 tgttataata tcccctgtaa agattatttt gcctgtaagt ttatccattt tactttcctt
    10321 ttttgtttag ataccttgcc attctaaaaa atgttaacca gtatatctta aagagttcaa
    10381 acttttcatt attattaagg tcaaatggga aaccctttat gaaactacta taaggagttc
    10441 ggaagttttg tattatcggt tcaataatgt ctatattttt caaattgtcc catattttta
    10501 ttttttgaag ttcttttcca gcaggtttat ccttcatctc tttaagttta tcttcaatat
    10561 cttttatttc ttttgcatcc attactattt tttctattct tcctattaag gaattcgtta
    10621 ttttcttttg ataagtgttt gcttccttaa ttgcttcgtg ttttatgttt tctatcagta
    10681 tatccataag tattgaggta agaattttgt taaatgttgc tgatgtattt cctgttttct
    10741 ttaattgttc ttcatacttt acacctttat attggctttt atcagtcagc cagtttatct
    10801 ttactcttcc aaatcccaat tctctttttt caccaatacc atccctttca agttctgaca
    10861 gttttttaag ttcaggacta ttttttaatt ttattttgaa tgatgttcct tctttaaaag
    10921 atatttctct ttgtgtttta catttccata tacccataaa tccttcagaa aaatcaatat
    10981 ttgcaaatgc tttttcttca atttctattt caccaccaga aaaatattgt tcaagatatt
    11041 tcttcaatct ttctattgaa acttccggga aaccatattc attgtatagt atgataggag
    11101 aaatagcagt taaggtaaat tctgtgtcag taagatttat tttttcaatt tcttcaggtt
    11161 cgccaaaaga aaattctatc tctccatact gggcagtttt tgaacgacct atattttcag
    11221 taaatttctc cccaaaacaa tccttattct cctcaaaaca attcttaata aaagaaaggt
    11281 aagatacatc ccctattaaa tatcctttga attcctgtcc ttcatctatt gattcataat
    11341 aaaaaatacc tccttcaaca ctatgacccg cagttctgtt ttctcttgta ttgtggaaat
    11401 aaaatctttt gttgattgca catttaacaa tgttattacc attataatca acaaaaccac
    11461 ctaatggcct ggttttttct cctggttctt cttcaaatag attatatata ttgttttcag
    11521 aaggtaattc agtaggtagt gtttctgctt ttttgatgtg aattgataaa ggggcagggt
    11581 aaaactcttt ttcttctttt gaaggatagg caggtgttat ggtgagtttt ccatcaagaa
    11641 atagttcacg aaataccttg tctttatgtg catcatttcc caaattaaat ttctttataa
    11701 acttatttgc aaggatacct ctaatgaggg tccctggtat atattcctca gttgaaacag
    11761 tgttctggtc tcctgtctgc tttgtaatta ttatgggaga caatgttttt atagtaaatg
    11821 gaagtttttt aatttcacca gttttgccct gacaggaata taattcatta gaaacttctt
    11881 ttgtattatt ttgttgtgaa ggaatagatg ttaagttttt aatttcttta tcaatatcta
    11941 ctatagaaat tccttctatc tcacatttta ttttaccaag tcccctgttt ctcctcactc
    12001 ctattctttt caggtttata actgcaagga aaaggaggga cttttcatca tctgttatgt
    12061 ctttatcaga gattgtccct tcaaattcta taaatggttt tagaaccctg taagttctta
    12121 atgaatgggg ctttgctaca cctttttcat ttatggctgt ctgttgtctt gtttcagtaa
    12181 agtatttaat tatgttttcc tttgataaaa caaatctgct atttttatct ttccttgctt
    12241 cttttatcag ttctgttata tttttatagt cctttataaa aagatttgaa aattttgttt
    12301 ttccttcatt agaccctgtc ccaaaaattg attcaagata gttgctatta gatataccaa
    12361 gtatttcaat tatttctgtt gcactttctt ttaatagtcc tttaattctt tttgctggaa
    12421 taaatggtat tccaaaatca tcaaaaataa tgtcgctgtc tataagtaca gatgcttctc
    12481 ctgaaccaac aagtgtataa gacaatgttt taatttttag tttcattttt ccctcacaaa
    12541 agttcttttg gataaaaatc cataagttct attatgtcaa aatatggtgt tttgtcgttt
    12601 actcttatat cttcgctata agggggagca taaggaattt cgtaaaaagt gtacccttta
    12661 atgtccatct ctttaacaaa catttttatt tctgattcag attttgttgt aacttcccgt
    12721 agttgttcta tttttgttct tgtccaatta ttgaaaaatt ttatacctcc tttaagatgg
    12781 taaatactat tttcctgagt gttattatct attctatatg gaccaaaagg caataggcca
    12841 taaggtattt taaaacttct ttctctgatt tcttcaagtg ttcctgaaaa accccttgaa
    12901 gagataagaa aatcaagata tgaagaattt tcttcttttc ttgattcttt ttttgcattt
    12961 tcacatagtt gttcagacaa ctgatatgca cggtaaaaag gatattttgt cttaacaata
    13021 gaaaccccag cacatgcaga aagtttttct cccttgattt gttgttcact aaatttggtg
    13081 ataaattttt cagcaagata aacccccaac cttccatcac aaacaaaggt aatatcatca
    13141 ccacctatta taattggtct tattggcaaa atggttttac catttccatc tgtgtgaaaa
    13201 tggaaattgt ttttaggacc caatattcta ttttcaatta aaccaactaa atcttcaata
    13261 gtttctttaa atgctttctg ggtattttct ttaactctta tagataagtt ccttagctct
    13321 ttaagggtat tgcatttttc aaacctcttt cccatgttat tgccgtctat atggacaact
    13381 gctatatagt ttttgtgttc tacctgacca agtttgtcaa tctcatttgt gaaggtatat
    13441 gtctctccaa gttcatcttt aagcatatct tgtatatgag acaatgactt ttctcctgct
    13501 tctattttta cctttgagac atttgaaatc catttatctt tataataaat ttcagcagat
    13561 tcaccagaaa aaggacagtc agcagtaatg ccatattttg gtaatgttat tatagggaaa
    13621 tagatgtttt tatttttttt caggttttta tgtatctctt tcattgaatt tgtaaaggaa
    13681 tttgcagagt tgtttaaatt gaaattcgga tttattccaa aagaggttct taatccaggt
    13741 gttttttcca acaagatatt tgtgaaattt ttaataaatt ccttatgttt tccattattt
    13801 acttcaaaca aaataagtgc attgcctcca ccaatatatc caataccata tggggaaaag
    13861 ttcatataag atggattgtt tttccaagta tctaaattag gagaggtact atgaaaagtt
    13921 ctgagtgcat ctttcaaata ttcctcataa atttgttcaa caagatatga tgcacctaca
    13981 ttttctataa gtttgttgct tgaaaatata taattctgaa ttgatactgt atcaattaaa
    14041 acgcctacca tcttatcctc ctacgaaatc tttaattttt gtctctatat cttttatgtc
    14101 atcttttcca aaaaccttaa aatgttcttc tgcagaacca gtaattccct ggatatcttt
    14161 ttcaagttct aaagcactat tagaatccaa tccagtaatt agaacagatt tggcttcatc
    14221 tccaccaatt tgttttgtcc tgtgtattac ttcaaatccc tttgatttac atagatattt
    14281 ttctattttt ttattcctga aaccagcagt tgtaacagat ataccaatca gttggtagcc
    14341 ctttataaga aaaacatcta attcaaaatt tctggtattg ggcttatttg cttgtaaacc
    14401 acatccataa ccagtaatac cttctatgtt tttcacctta tccaaaatat accattcaag
    14461 ccatttccca tcaagaaagt caactacact ggttttgttt tcaagtgctt tgttaatatc
    14521 tgacggttgg ggggtccaaa gagtattttc atttgtaata atttttttct catcaggaaa
    14581 tgaattaagt atttccttga tatttggggt taaacttgtt ggaaaattcc caactatttt
    14641 tgaaagagat tctttttcaa tattttcttt aaatttttta attgtatcta ttaaatttcc
    14701 atcttcatca taaaaaatct tgcgtaaata ttcatctttc cattctataa attcattaat
    14761 ttctccagta tttaaaagat ttataaggat attaagtacc tttccaaaat ctttttccat
    14821 aaagtatctt acaggttctt ttatattata tccatgtaaa gaaaggagtg tactaatatc
    14881 taaagaaaca taagttctca tatcccctgt ttcaggttca attttcctat catcataaag
    14941 caatttatat gttcttgcat caagataact aaaatcagca taagggaatt tttcctttat
    15001 aaattcgtgc agacatgctg ccatgacttt tgttccacct gtataattaa gatgaattct
    15061 ttcaaaattt tgggatgaaa ataatttttg taaatcaata cggatatctt ctactaatga
    15121 gacattagaa aggggaagaa attgaaattg ttcttctctg catccatttt ttatcaattg
    15181 ttctttaata gattctgcga atttataagt tccagattgg tttatttcac atctttcctc
    15241 agaataaact aaacagaact gcctatcttt gtcttttaaa tagtccgcta caacataatt
    15301 aggtaaagga tttgttccaa ttatcaaaac aatcactttt atctcccgta gtaaagcatt
    15361 aagacaattt ttatcttttc aacgctttct ccaggtatgt ttctccatat atttgataac
    15421 ttaccccagg cggttcataa tagaaaaaat caacaccaat atcttttaaa aaatttactt
    15481 gctcctgaaa cctattgaaa ttttctgcaa taagtttttc attatattct tctccttgac
    15541 aaaaaatagc agtgattttt gctcctttta aaacatttaa actctctgga ttaggtttag
    15601 gacggtttag ttcatgtatc atatccgaat aatatatagc ccttacagat gggggttttt
    15661 taaacttgct taaataccga gccatatatt catatgttcc atatatatcc gtcatatttt
    15721 cactatattt ttctatcggt atattttcta aattgtttaa tatctgcatt atttcactcc
    15781 atttattacc ttttaaaggg aattcataaa gtttatcata ccctttctgt cttccagtta
    15841 tctttccaat aacaaaataa taatttttcc aataaagacg aaaatttttc ccacttataa
    15901 ttcgtgctat tccagaagat aggtctttta ctgcccactc tttataattt ataagtttac
    15961 ccagattatc atatccaata taactattgg tcacatcaat ccagacaaaa acaatttctg
    16021 aagaatatgc tggcggacag gtagagaaaa tatttaaccc tattaacgaa aaaaaaataa
    16081 gaaatttata tttttttatt ttcatctttt ttctcaacaa tctctacatc tataatttta
    16141 ttgttttcat agacgatgtt tttctctttc ctgtttttat aaaattgaaa aatagggtct
    16201 ataagaaaat aacaccagca agctataact attatataaa ataaaatcat ttttatgctt
    16261 ttaagaaggt atttaccgac aggagaaaac cattttaaca tcgtaccgca aagtgttctc
    16321 ccatatggta aaggaacttt ccattccggc ataacaaatg gttttttata aaattgaaaa
    16381 ctaaaaattc cacaaatgac aggaataaca actgtaaccc atcccattgc ttcaggaggg
    16441 atattagaag tgagtgattc taaagaaaat gtttttgaag caggcccaaa tagtcctctt
    16501 actaatagtt ctctttcaac ttttactgct tcaacattat aattatagtc tactaagaaa
    16561 attttgaata aaccataact gataatagga accaatatta aacctaaaat taaatttttt
    16621 aaggatgctc ttttttcatc gggagaaatt gttttgtttt ggtctggaat tcgcagttta
    16681 agaatattat caagcaaatt attacttaaa accataagga atacaataaa tccaatattt
    16741 tctgtaagaa caattcctgt tgaccttata attgaatttg ttccgccgcc ctgcatacca
    16801 gaaacagccc agtagacaac aacaaaattg atgattatac cggcaatttg aattagaact
    16861 tctggtctaa aaagatattc tcgtaataca cggccaataa aaagtagaag agcaaaaaaa
    16921 taacgaatac actcccatat tattattaca aatggagaaa tcaaaagaag gaggaaaagg
    16981 ataaccaacc aggcaatata atatatactt ttaaaaatgt gataaataat aaggaatgga
    17041 gctgtaataa accatggttt tttcccttca ccaattaagg tttcagtaat tttagataca
    17101 gggtctaatt tcccaaattc ttctccaaat ttctgcaaaa gagaaacgac aaaattaatt
    17161 atagtccacc cgattatcca ttgccaaacc accgataagt gagaaaacca tttaatcata
    17221 aatttccccc cttttgaact atattaaaac atcaaaaaaa attgaaaatt ctcttttatc
    17281 ctttcctttt gtttttaatt tatcacttta tttatttttg tcaagaggaa tgttattacc
    17341 atgtgattat gtgacagttt ttaccaaata aaaatatgac atttccactg gcccacccgt
    17401 agagggccct catttttaac tacgaattat gaactatgcc cgagagatga gaagtgaagg
    17461 gtaaatgttt ttactatata tattatatac tgtgctgtgc agttttttta attttttctg
    17521 attcaaatag acctttttgt ttgtatgagt tagacatacg ggtaaaaatt ttttcataag
    17581 ggggtcaaga aaaattaagg atgcgaagaa aaggataaaa ccctccttga tattgaaaaa
    17641 caacagttct ctatctaaat tgaggacttt gcaccatcaa ctgctaaaac atcataaaaa
    17701 gcaggtttta tcttgaattt ctcaaaatag gcactttcaa gtttttcttt gaattcttct
    17761 ataattattt ttttaatcaa aacaatacag catcctccta atcctgctcc tgttaatcta
    17821 cttcctgcaa caccttcaaa attttttgaa aaatcaacta taaaatcaag ttcattgcaa
    17881 cttacattat agagttcttt taaactttta tgagaagaat aaagaagttc accaaatttc
    17941 tcaatttctc catttttcaa aagttcaaca ctttttttaa ctctttcatt ttcttcaaca
    18001 atatgtttac ctctcctgta ttctgtttgt ccaattaaac ttttatattt ttcaagtatt
    18061 ttaaaatcaa cttgacctaa attatcaata tctacaaatt tttttataat attcacaacc
    18121 tctccaactt cttttactct ttcgttataa acagaatttg aaagtgttct ttcttttttt
    18181 gtatcaacaa gaagtaattg ataatcacct gttttaaatg gaacataaga ataatttaag
    18241 ttattgcaat taagaaatat tgcattatcc tttttcccta aataaataat aaactggtcc
    18301 ataattccac aattcactcc aacaaaatta ttttcagcac accgtgcaat gtttataact
    18361 tgaatatcgg gaatttttat attgaaagtt ttcatcaaaa tagaaattgt tccaatctca
    18421 acagaagcag aagaactcaa accactacca acaggaatat tacccccata agcaacttca
    18481 aaacctgaaa tgtcaatttt acttttttca aattccttta ttactccaaa aatgtaatta
    18541 gcccattttt tttctttatt aaattttata tctagaatat caacttctaa ataatcatca
    18601 taattaaaag aataaatttt aattttttta tcttttcttt tccttccaca aaaatatatg
    18661 tatttattta ttgcaattgg tagtacataa ccaccctgat agtcagtatg ttctcctatt
    18721 aaatttactc ttcctggtga ttttatatta aaaatatcac catccccaaa aatttctata
    18781 aattttcttt taatcttttc tttcatagaa ttgctccttt tataaaaatt ttactatctt
    18841 gtatttttaa tgtaaaataa ttagtataat attcaattag agaagtagag aaacaaatag
    18901 tgagaaaagg agagaaatgg aaaaaaagat tgaaaaacaa ttatctttac catttaaaca
    18961 tgcttttgga atgagtataa gaaatataaa aataagattt ggaagagcaa taattattac
    19021 tatgtctata cttttaggaa ttgctttttt gatgtctatt ttaacaggca atgctttaac
    19081 aatgtcttta attcaaaatg gacctgaaga aataagaatt cttttgcaaa aaaatattga
    19141 agaagttaaa ataaggcaag tatggcttgt atctctttca cttcttgtct gtgtggttgg
    19201 aattataaat tcaatgctta tgtcagttac agaaaggtca aaagaaattg gaacaatgaa
    19261 atgtttagga gcacttgata gatttgttat ggaacttttt cttttagagt catcaattca
    19321 cggaatactt gggtctttac tcggtagtat agttgggata gttgtaatga cacttgttta
    19381 tcttatacaa tataaaggac tgattttaaa attatttcca tttgcagtaa taggtaaata
    19441 tctcttgttt tctgtcattt tgggaacatt tcttgcagtt attggtgctt tatatcctgc
    19501 atatgtctct gcaaaaatgg aaccagcaga agcaatgaga agagaagttt aaaggaggag
    19561 agaaaatgcc aaaagaaaca atagttagaa caatcgggtt gaagaaaaat tatatgttag
    19621 gaagggtaaa acttgaggtc ttaaaaggga tagacattga aattttaaaa ggtgaatata
    19681 tctcaattat gggaccatct ggaagtggta aaacaactct ttttaatatg attggtggac
    19741 ttgataaacc aacagaaggg aaagtttata ttgatgaagt tgatgttgct caattagatg
    19801 cttatgaact tgcctggtta agatgcagaa aaattggata tattttccag acatttaatt
    19861 taatacctgt gatgagtgct ttagaaaatg tccttttgcc aatgattttt gcagggatga
    19921 caactgatga tgcaagagaa aaagcaaaga atttacttga aactgttgga cttggagata
    19981 gattacatca taaaccattt gaactttcag gaggtcaaca acagagagta gcagttgcaa
    20041 gagcacttgc aaatgaccct gctatagttc tcgcagatga gccaactgga aatcttgact
    20101 tgaaaactgg aaaggaaatt ataaatttat taagagaaat gaacagagaa aaacaggtca
    20161 gtattattac tgcaactcat gatttaaaga tgttagatgt ttcagatagg gttatatggt
    20221 taagagatgg aataattgaa agagttgaag atagagaaaa cattaatatt aaagtaggag
    20281 aagttgaagg agaggaaata taattttgaa aacaattgta ttggttgata gttcaaaagt
    20341 tgtacaatac aaaagagttg gaaaaacaat tttaaattgc ttagaacatt ttgggatatg
    20401 gtatgaaata attgatttga attgggcagg aatttcttat gaagaaattc ataataatgt
    20461 ttctgttata attttagggc aggaaggaat tagcagttca atttcagata cagatttctc
    20521 tttaatttta aaaagtgttg aaaatggaac tggactggtg atttttgatg gttatttaaa
    20581 taaatattca gaaaagtttt taaaaacaat tggaatagaa aatttttcct caaaaaaaac
    20641 acaaaccgtt tctcttgaaa aaaactggat aacagaaata tcaccagatg ttgtttattt
    20701 aaaaagagat actcttcact actgcccatc tgtgaaaagt gattgggata tttttatgaa
    20761 aaatgaacag ggtaatggct gtggattttt tagaaaatat gggaagggaa aaattatctt
    20821 ctggacaaca ttaccttcaa tttttttaga tgagtattta ggacatacag aaggacttga
    20881 tggtgttttc tttcggtcta ttatatggac ctctaaaaaa ccatttataa caaaaacaat
    20941 gcctccattt gttacgggga gaattgatga tgcttctggc tcaggaagtc cagtaagtaa
    21001 atatcctgag acagttaatg gtttaaaata tttagatatc ttgaataaat acaaatttat
    21061 accaaatatt ggtcttttca tagatgattt aaaagaagaa gatgataaaa aaataagaga
    21121 aaaacattat gatggattag cagaattttc tccacatgcc ttttctgacc ctgaaaataa
    21181 aaatgaattt ccaatttatc ttaaacatag tggggaagaa ttttctgata gtgaattaaa
    21241 agaaaatttt gaaaaagtgg atattaaatt tgggaaaatt ggaataaaca tttcaaaaac
    21301 actaaatgca cattttggag aagtgggatt aaaaacattt ccttttttga aagaaagagg
    21361 tgttaaatat tttatgaatt caataagagt tgggaaaaga tgggatgacc ctttatctaa
    21421 aatctgggaa attaaacctt ataataagcc gtttttttca ttaggttata ttccagaaga
    21481 tgagtatttc tttaatgtag aagcatcacc acccaaaatt gacattagtg cttctgccct
    21541 tgattcagaa agtcctgatt ttgattttct ttatggctgt actactttct ggaatgaaaa
    21601 taaaaaagta gaagtggaaa aagcagcaaa aagaggtatt ttcaagatac aaagaggact
    21661 tgaaaatggt ttttttggtt ctctttcaac tcatgaacag agaatttcta atgttccaat
    21721 tttacaattt gaaaacataa tttcatctat ttctaatgaa ataaataaaa tgtctgtaat
    21781 tttcaaaagt tatgatttta tttcacaata tgcagaaagc agagcaaatt acacaataga
    21841 taaaatagaa ataacaaata aaatttctat tgttctaaaa ggtgaaaata aagttgaaca
    21901 acttctttat cttttcactg aaacaaatgg actgccaaaa acaagttttt taaaagtgcc
    21961 aacttttcaa aaacaaatta ttttaaattt ttctatttga tttaacattt acggagaaaa
    22021 aatggggaaa ttaaaaatag gttttattgg atgtggttct atctcttatg gacatttata
    22081 tcaaataaaa aaaattgagg atgttgaaat aaccggtgtt tttgacccta aaaaagaaaa
    22141 tgttaataaa tttttatccg aagcagggga aggtaaaatt ttcactagtg aagaagaact
    22201 tattaaaaat gtagatgcag taattatatg ttctcctcat acatcacatt ttcctcaaat
    22261 aaaaactgca ctggaaaatg gagttgatgt tcttgttgaa aaaccagcag ttgttaatta
    22321 tgaagaagca attaaattaa gaaaattgat tgaaaaaaca ggtagaaaag ttgtagttgg
    22381 ttatcaaaag cattatatgg aattacctct atcagttaaa aaaattattc aagaagggaa
    22441 aattggaaaa attttatttt taagtggatt cctttctcaa aactggatag aaggaattac
    22501 aaaaagtgga agaatatgga gatttgaccc taaattttca gggaaagggc aacttactga
    22561 ttctggaagt cattttataa gtatgctgtt ttggataaca tctcttactc ctgaaaaagt
    22621 tggtgctttt attgaatttt ttgataaaaa agttgatatt aattcttcat ttacagttaa
    22681 atttaaagaa ggagcacttg gcagtttcag tattcttggt attgacccgt cttatagaga
    22741 agcactaatg atatggggag aaaagggatt aataaagttg agtttatttg gagaaaactc
    22801 atatattcat tacaatggag aaaaagaacc aaaaaaaatt tcagtctcaa aaccaacaat
    22861 tatttctcct gttgatgact taataaaatg tataagagaa aataaagaac cagaaacaga
    22921 catttctgtt atagaaaaag ttgccttact ttcagataaa atttatgaga gttttaataa
    22981 agaaaaaatt gtaaattgtt aaaaaaaatt tattcagaaa agataaccta tggatatatt
    23041 gaaaattaat tgtgaaaaag tatgtgatta tcttgtttca tttttaaaag aagaagtaag
    23101 taaaattggt tttaattctg ttatttttgg gttatcaggt ggacttgatt cttctcttgt
    23161 tgccttttta tgtaaaatgg cttttggaag aaataatact tatgggataa tacttccata
    23221 taaagataca ccaaaagagt cagttgacca tgcttatctt attggtagaa ctttgggaat
    23281 aagacatttg agatttaata tcactcctca aattgattat tattataaaa atttcccaca
    23341 ggcaactaaa ttaagaaagg gaaacaaaat ggcaagagaa agaatgagta tactttatga
    23401 tctatcttct cattttaaat cacttgttgt tgggacaagt aataaaagcg aaatatattt
    23461 aggatatgga acattacatg gagatactgc ttatgatata aatcctgttg ctgaccttta
    23521 caagacacaa atttatcaat tatctgaata tttaaaaata ccagaagaaa taagaataaa
    23581 aaaaccaagt gcagaattat gggaaggaca aacagatgag gaagaattgg gattttccta
    23641 tgaagttgca gataaagttc ttttttattc ttttgaaaaa aatatgaaac aacaggatat
    23701 tatgaaagaa attaaaattg atgaaaggac ttttttgaaa attattgaga gagtaaaaaa
    23761 atacgagttt aaaagaaaac cacctttaat ggcaaaaata cctgatgaaa ttaagtatta
    23821 atagaaatta gaagtaatat tactgcctga ttttgttctt tcgattgctg tttgaattct
    23881 ttcttttaaa tcggagattt caattgtatt tgtgttttta tcaattgtta aatttattga
    23941 atttcttaaa tgtactcctt ttattaaata ctgtttattc tccttttcac caagaagccc
    24001 ttcaagtgtt aaaaaattat ttttaagtgt tattttcata cccatttgtc cataatagaa
    24061 attagaactt ccaattgttt ttataggatt tcctcctgca agagttgcaa agaaatttac
    24121 tgcaccaaaa ttcataattt gttttattcc tttttttctt actgacaata tttcactttc
    24181 aataatataa ttttcatctt ctttttcaaa attgccatct acatttacta ttccagatat
    24241 atggatttct tttgtaaggg ataaaataag tttttcaaga ttcatttctt ttattgaaaa
    24301 atcaaaattt aatttttcac caatttcata atttactttt ccttttccat cataaaataa
    24361 aaaggtaata taaccatcat ttactgaatt caaatttcca tttatgtttt caattttcaa
    24421 attgtcatag gataaattct cacaggtaag tgtgccagga aaagaaattt ctaaattttg
    24481 aatatggtaa ttattaaagt caaaagaaga aacaaacagg tttaagttac cagttgtttg
    24541 aaattttttt ttactttgct taaagagaaa tgattgaaat ttgcagttcc accagatata
    24601 tcaattttat atttttctct tatatcctct ggcaaaattc ttagcaattt ttctatttgt
    24661 attttctctc cagtaaaaga gagattgaaa ttttcattat ctatttctcc ccaaaacctt
    24721 aattcattac tatctataaa agcaactcct ctttcaattt ctcctttttt ttctgttgga
    24781 tagaaacacc cttttaaaag aacaattatc ccttcacctt tttcaacttt taattcttca
    24841 actttaatat caaaatcaat tctttgtttc tcatcttttt tatataacat tatatttcct
    24901 gaaacattac cttctaaatt aactttcaaa ttaaaaaaac tacatacctg ttttatatca
    24961 attttattac attttactat tacatcctgg tctgtttttc cccagttttt caaatttccc
    25021 tgtacctgta tatcaccaat ttcttcaatt tttatatttg tattttctat attcacattt
    25081 ccacttttta tatttcctga tatagtccca tttattgtaa tatttcgctc ctgttgttga
    25141 tttcctacct ctcctggtcc tgcaaaagac acttttgtaa aagtaggttt gattttaagt
    25201 tcatttaata aaataaaaaa agcattttgt ttatatgata aattctttat ttttgccccg
    25261 gaaaaagaga ggtctatatt ttcaattttt aatgggatgt tagataaaga agataatttt
    25321 ttatttataa ttctttcagc aatttttaaa gagataaaaa cgaaaaatac caagaaaaca
    25381 actattgata taacaatttt ttttcttttt ttcattttaa atattttcca aaaaattcaa
    25441 atgcttttct acctgaaaaa gaatgttctc ctggaaaaat ttcaatccat aagttatttt
    25501 caactcctgc aactttataa acatttttta ttttttcatg tgccttttta gctgaatcaa
    25561 tccagaaaca tgtatcatta actcctgatt caattaaaag tggttttgga catatacatc
    25621 ctattacatc tccaacatct gcatatttat aaagaaaagg aacaatctga cttccacaga
    25681 agtttggtct gcttattgca tagtgtaaag tcgttgttgc ataacatatt atatcacctg
    25741 tctttattct ttcatcaaga agagttaaat aagttgccat tgtcccacca aaggaaagtc
    25801 ccatacaacc tattctttct ttgtccacct cttctcttgt aagtaaaaaa tcaataaccc
    25861 tcataccatc aaaaatattt gctccaagta aagtccttcc aagtatgaga tgttgaagaa
    25921 aatatacatt acatttatct cttccaggaa aaaaagtgtc ctgatatccc cctctctctc
    25981 cgaaaccacg ccagtcagga catatagtta taaatccatt ttcagccatt tgctgtccat
    26041 aattatagtt atgcattttt atattattta taatatctgg attattatta tgaacaccag
    26101 caacagggtc ttttccataa gagccatgcc catggcagca taaaattgca gggtatttct
    26161 tcttgtttcc tttcggaatt aaaagatata aagggacaaa acaatctttt tctgattgta
    26221 ttatccattt ttctcttata aatttttctt tttcctcaac tgataagagt tctggattta
    26281 agtcagatgg ggttggaaat tcacctaata atttttttat tttttttatt aatttttctt
    26341 tccatataaa aacatcttct tttgttttcc ccttaaattc taatgatggc ttaatttttc
    26401 ctgcccattt ttcaataaaa ttatcaatca caacaggact atcaaattta tttggcattt
    26461 tccctccttt tttttatttt aacttaaaag gtaaaaaact tcaacttctt ttatcaaatt
    26521 tcaataattt ctgaacaata aaaagaaatt gcagagacaa ttaatatcat agtgcatgaa
    26581 aatatggaaa ttcaggatta tataattttt gtactcgagg aagaacaaaa caatcataca
    26641 ggaattaaat taatacttgc tgtttcatcc ataaaactgc ccgctccatt gtttcttctg
    26701 gggtataact tggtttataa ccaagttttt gtgatatttt tctaatgtca gggcacatat
    26761 gagccttaaa atgaaaattt gctcctgggt caggatttat ttttgtgcag tattcttgcc
    26821 aactatacca ttgtatagga attttcacac catagatttt ttcgtatgtt tgaacaaaat
    26881 ttagagatgt aatagcgtat gcagaaccga cattaaaaat ttcatcagct gctttttgtg
    26941 attttaaaac agcaaggaaa aatgcttttg ctacatcttc agcatcacag ggaccaatta
    27001 atgtttgacc tggctcaggt aaagggacag gatatccttt tttatggttt tgatgatttt
    27061 caatgtttct atctccataa cagtctaggg gaatttttcc aggaccacag atattcggtg
    27121 gcataatagc agtaaaaata attccttctt ttttagcagt attttttaat tcaagcatct
    27181 ctgcatacct ttttgcatag ttggagaaaa tacagggtga ttgtgtttgt tcaggagttg
    27241 ggacaatttt ggactcccca aacatccaga cagaaccaca taatatgaaa tgtttacagt
    27301 aaattttaga tgtttcataa actaatggcg cataagaacc aagaatatca actactattt
    27361 ttggctttat ttctctgaaa agattttccc attcaatttt gttgttatca taattttttt
    27421 ttacaaattt tattctaaaa ttttctggag tttgtttaat tcccctcgca actacaaata
    27481 tctcataatt ctctcgtaaa agaaatttaa caaggtttat tcctatatgg ccagtacctc
    27541 cgattatgca aattctcata atattctcct ctgtttaaaa atcaaatcta tccactttat
    27601 attcgggcta tttttgtttt tatttattaa atttaattat ctaaaaatgt cgaacttcta
    27661 ctatttctcg aaaacaacat atgcgttcag taaaattgtg ctatagcagg tcgtaaaact
    27721 tttaatattt ctactatgcc ctttttcttc ccgcaaagag gagaggattt aggtgagaga
    27781 tattattcat tttttattgc cccttctctg tacttttata cctattccaa ctgttctatg
    27841 gaggttctaa aaatataata gcatttgtta aaaattttga caaaattcta aattgtggtt
    27901 taattttaat atgataaaaa aatttaaatt tatatgtgaa ccacaagttt tttatgggaa
    27961 taattctgtt tctttacttc catcaattgt taaagaaaca aaaagaaaaa aattacttgt
    28021 tgttgtagat tcaaacttct caaaaacaag ttttttcagg gatatagaaa aaaaactttc
    28081 tggtgaaaaa attgatttcg ttgtttttga taaaatttct ggtgagccaa caactgatat
    28141 tggagatgaa tgtgcagaat tcgggaaaga aaataaatgt gatattgttt gtggaattgg
    28201 cggagggagt agtttggatg ttgcaaaagc atcatctgtt ataattacaa atggcggatg
    28261 tgtaaaagat tatcagggat taaataaagt ccctggaccg ggactgccta aaataatgat
    28321 accaacaact gccgggacag ggagcgaagt tacatttact gctgtattta ttagagagga
    28381 tacaaaagaa aaaggtggaa taaatagtcc atatctttat cctgaatatg caatacttga
    28441 ccctgtcctg acagtttctt taccaacaaa tataacatcc tcaaccgggc ttgatgctct
    28501 ttgtcatgca atagaaagtt atatttcaaa aaattcaaat ttcctttcag aatctatctc
    28561 tctttgtgct ataaaatata tctgggaaaa tttaccaatt gtttattcaa aagatgagga
    28621 cttaaaggca agagagaaaa tgctttatgg cagttttctt gcaggccttg gacttgtaaa
    28681 tgcaggggta actgctgtac attctatatc ttatcccttg ggaggtgtct atagagttcc
    28741 acatggagtt ggaaatggac ttcttctccc ttatgtttta gaatttgata ttaaaaaaga
    28801 tatttcttat actgttgaaa ggaaattatc ttcaattgtt gatattgtta gaagtgactt
    28861 caaaggagat gaaaaagaaa aagcaaagtt tttagttgat gaaataaaaa atttccttaa
    28921 attattcaaa atgcccaaat taagggattt taatattgat aaagaaaatt tctcaaaact
    28981 tgcagaagat gccttaaagg tctcagttcc tataatgaac aaccctgttg caataaaaaa
    29041 agaagatata attgaaattt ataataatgc ctattaatca aatctatatt tatcctcttc
    29101 aacccagaca atcttccact ttttcccttc ttgcttaaac ttaattaaaa atctccctgc
    29161 ttcttttata atttcaccag aaggacttat tgcttttcct tcgccaaata ttttaacaac
    29221 cgcagtttta tcaaaaattt ctatattcaa atttctaata gaaatatcca ctttttgata
    29281 atttttgaga atatttttga ctaaaaaaaa taatgttccc caggtatttc catattcatc
    29341 tctatattca aaagaaaaat gtttcataaa ctcagtatag ttattatttt ctattgcttt
    29401 ttctgctatt ttaattgtct gttttattct atcttcttct gatataaaaa taaatttttt
    29461 taataaaaga aaaatacaaa ttaaaattaa aaaatataca aaaaattttt ttattttacc
    29521 catattactt ttcctataat atttttaata gagacatcaa ctaacaagtg ttgtgaaata
    29581 tccatatctc tgttatctcc aattacaaaa atatatccct ttttaatttt tatttcgggc
    29641 atattccagt tatcattttt atttatcaca taaggctcta ctaacttttt cccgtttata
    29701 ataacatccc catttattat ttcaactctt tcgccttctt ttccaataac tctttttata
    29761 aaataaacat atggtctatc tgatgttctg aaaacaacaa tttctcctct ttctggttct
    29821 ctgaatttat aaataatttt attaacaaaa atgattttgt tatctgaaat cgttggcttc
    29881 atacttggac caaatgtctt aaatggctga attaaaaatg cccttgtaag aaataaaact
    29941 aaaatcagac atatccagaa aaatattctt tttaaggaag ggattcccat ctccacaatt
    30001 ttatccattc ttttaagtat ttaccagcaa tttctttatt tcttaatatt aaaattgtct
    30061 cataattttt ccttgctgat actgttggat tataagagcc agttattact actttttcat
    30121 ctattacaaa aaacttatca tgaagaaaac cagccatatt attttttctg acatttattc
    30181 cataatcatt taatatataa aatgtggagt aatttgatgt attatatctt tcaaaaatcc
    30241 catttatatc tacacctttc ttttttttgt ctataagatg ccttgcaata ttttctgatg
    30301 agaaataaaa atgtgaaaaa taaatagaac ttttcgcttt ttcaatttct tcaattattt
    30361 tttcttctaa attttcttca ggagaaaaat aaatttccaa tgtttcactt aaaaaaggac
    30421 ttttattatt ttctttatta ttccataatg aaaaaaagtt ttctttaaaa tactcagaga
    30481 tatttttatc tttgattata agaaaattat tatgaaaaag atgaaaatca tttttagtga
    30541 aatttccaga accaataatt gttatctcat tatcaataat tataaatttt gaatgaaaga
    30601 gattactccc tttatagacc tgaatacaac tttgtgaaaa tggcaaatta ctttctttcc
    30661 caataaatat ttttaattca tctaattttt tattcttaag aatttctatt atttctttat
    30721 aattaaaatt ataacttgca ataaaaattg aattttttgc ccctttaatt acctttaaaa
    30781 tttcttcatc tgcttgtttt gacggagtaa aatagactga aattgaagat aaaattgagt
    30841 ttataaggat tataaagata aacaaaaatt ttctcataaa gatattataa aacaggaatt
    30901 aaagatgtaa aattttagga ctttttactt gaaaagtttt ctataagtga tttagtattt
    30961 tctcttaaaa ttttcttctt tacttcatca ggtatatcag aaagtaaaat ag
//
LOCUS       DAOOVW010000019        29985 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_1046_length_29985_cov_8.164250, whole genome shotgun sequence.
ACCESSION   DAOOVW010000019 DAOOVW010000000
VERSION     DAOOVW010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 29985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29985
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1046_length_29985_cov_8.164250"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..702
                     /locus_tag="PLW95_03950"
     CDS             148..702
                     /locus_tag="PLW95_03950"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOV21817.1"
                     /translation="MAIISIGNKIKKLRENRGIHLRTLAERVGVSPSFISQLENNKTS
                     PSLVTLKNIADALGTTVSFLIGDEKKETETPVMKKEEWIFQQMGEGIKMFYLTFPDPY
                     KQIEPLLFVMEKGASSGENVYQHFGQEFVMVLNGKLEVTLAEKKYVIHSGDTIYFNSY
                     IPHKFKNVHKGVTKVVWVDNPPTF"
     gene            767..1465
                     /locus_tag="PLW95_03955"
     CDS             767..1465
                     /locus_tag="PLW95_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21818.1"
                     /translation="MEELLISKTKGLTEEIEYFANEIIGMKDTDKMLKRIGDEMKYQN
                     YDYVEEKEKMVCGEISGIKKEGSFYLISDLGQGIEANGIHNGVITSIFVGALIKRCLL
                     PLRNDLNVIISDEEIEKLFQKISYKGAKGIIICKPTNYQIYKINKEKDNYEKLNGYYS
                     FVHTLVSTLEEERYETVLREKEMPVTTTIPIVEFGGDDTFEIGKIEKTIYIITKVVIR
                     CVGFPLFGWSADEI"
     gene            1455..2648
                     /locus_tag="PLW95_03960"
     CDS             1455..2648
                     /locus_tag="PLW95_03960"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HOV21819.1"
                     /translation="MKYKTKILVLNCGSSSLKFKVISFPEEIEIVSGEAEKVGVKSTT
                     SSRIKMRIENDTYEIKENLPDHFSAFKTVVNYLQGKINFDIIGHRYVHPGNEKKHSLI
                     INEESIRYLESTLHFAPLHNPVSFSMIKFSQEIFPDIPQYAVFDISFHNTIQPEFSTY
                     ALPLELSQKYNIKRYGFHGISHQYVMEEALKFLNVEKEKVKIISAHLGTGGSSLCAIK
                     NGESIHNTMGFTPLEGLIMNTRSGDIDIGIVLYIMFLKKFTPDQMDNILNKQSGLAGI
                     YKETSDLREIASRVNDDGRAEKAFDMYVRRIKKYIGFYFLILEGFNVLIFTDTLGVEV
                     PLLREKICDEMDFLGIKLDKEKNKNSTDYCLKDISADGSKVKILVVPTNEELMIAREI
                     YRGIK"
     gene            2645..3679
                     /locus_tag="PLW95_03965"
     CDS             2645..3679
                     /locus_tag="PLW95_03965"
                     /inference="COORDINATES: protein motif:HMM:NF013067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21820.1"
                     /translation="MILLLNPSIYKIEITGSDDKSNSYEIEEIKKLSLYKDKTEAISI
                     RILWGGEFLRNKGLIDNNFIEKYKGLITFFPVYVPRIEKILKYLLENFQDIPIIPFFE
                     DKFFLNLPVEEKYYGINSFRIGRMKIRKRGFHGIFHEFVSEKFSSYKKIISIVLDKKT
                     TICGIKNRKPQLISIGFTPLEGIMGKTSSGDLDPGIVFYMMKELGYSIFEVDNILKKK
                     SGFYGLTRYNRDINEIYKNFYKKDEKVNFAFKIYKKQIIKYIGEAISVLDGVDAIVFS
                     GEYVKEMISFIYEIISEISFIGIKLGKISKFSDNIGIISIEKIPVFLNFYTDKEIIFY
                     QTEKVLKNFV"
     gene            complement(3801..3876)
                     /locus_tag="PLW95_03970"
     tRNA            complement(3801..3876)
                     /locus_tag="PLW95_03970"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3838..3840),aa:Pro,seq:cgg)
     gene            complement(3905..3978)
                     /locus_tag="PLW95_03975"
     tRNA            complement(3905..3978)
                     /locus_tag="PLW95_03975"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3942..3944),aa:Arg,seq:gcg)
     gene            complement(3982..4506)
                     /locus_tag="PLW95_03980"
     CDS             complement(3982..4506)
                     /locus_tag="PLW95_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006569883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HOV21821.1"
                     /translation="MMKKIFFPVDEKKLANLKIGDFIFLNGIFYTCRDAAHKKIFKLI
                     EENKNLPFTLNHNLIYYVGPTPAPPGKIVGSFGPTTSSRMDTFTPLLYKIGLKGTMGK
                     GSRNKEVINACRKYKAIYLITFGGCGAYLSKFVRKIELIAFEELGAEGIYKVEVENFP
                     AIVGIDIYGNYLYK"
     gene            complement(4500..5336)
                     /locus_tag="PLW95_03985"
     CDS             complement(4500..5336)
                     /locus_tag="PLW95_03985"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOV21822.1"
                     /translation="MKKISYKQIIKEVSNLIIDINYGVSKNIEEYLKKAYYQERNGLS
                     KKYLKIVLENIKISKKEKLPLCQDTGYPVVFVEIGSNVKIEKGPFPSLIDIINFGIED
                     GSKKGYLRNSVVEPIDRNFTGYNTPCVIHFFEGKENKLKITIMAKGFGSENTCKMKML
                     NVADGKEGIENFIVDTIKQAGSLPCPPIFVGVGIGGTFEMSPLLAKISLLKIGEKSKY
                     RNWELEIMKKINKLKIGAGGFGGKTTALDLKIETHPTHIAGLPVSVCISCWAHRVEKL
                     EL"
     gene            complement(5317..6057)
                     /gene="recO"
                     /locus_tag="PLW95_03990"
     CDS             complement(5317..6057)
                     /gene="recO"
                     /locus_tag="PLW95_03990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOV21823.1"
                     /translation="MPPIKSEGIVLKKFDFRETSVILSIYTENMGKIKGVLKGVRSEK
                     SKIAPLTFQPGSHIFTLIYKKKGELNLFSSPSLLNNFEIKEKKNSNVWFYILNLLDIF
                     TPENLQDNKILQLTLEIGNYLEKASNPEIVLTFFKTKFIKSLGYGFHLEKCISCNKED
                     KMYFFSGKLGGIICRNCNKKDINPVRISFPVLKIMRMFDTLDFDKIKIIKSIHKDILN
                     KLNFYINTTLDFHSDIDKIWWENEKNLI"
     gene            complement(6038..6619)
                     /locus_tag="PLW95_03995"
     CDS             complement(6038..6619)
                     /locus_tag="PLW95_03995"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HOV21824.1"
                     /translation="MGKLRKYFISGLVFIIPISLSIWILYKIIVFLDGIIGNLLKKFS
                     PHIYTPGIGFFSLILIILFIGFLTQNFIGKKLLRRIEVIFEKVPVLNRIYNFIKSIVQ
                     SVTSKKEQIFKGVAKIKFFDGSYTVGFITGESQLIKEEKFLSIFVPTVPNITTGFYVL
                     VPEKMVEKLNISVEEGFKIMISAGFAENATNKE"
     gene            complement(6621..7340)
                     /locus_tag="PLW95_04000"
     CDS             complement(6621..7340)
                     /locus_tag="PLW95_04000"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - diacylglycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HOV21825.1"
                     /translation="MKENKKNHDILSSFNSAIEGFFYALKTERNFKIHIFIAICVITG
                     SLFLKIPLSEFLIILIFISLVFGAEIINTSIENFSNLFTIENHKEIKKIKDIAASSVL
                     VASFFSFIGGYLIFVKYFPSGWRNIFENIANSQWHITFIALVIVISLAILLKFSIEKE
                     FSLSGGMPSIHSGIAFSIWTTISILTFKEFPIISFLVLILAIWVAESRISKKIHKVNE
                     VIIGGIIGILLTILIFQVFWR"
     gene            complement(7394..7843)
                     /gene="ybeY"
                     /locus_tag="PLW95_04005"
     CDS             complement(7394..7843)
                     /gene="ybeY"
                     /locus_tag="PLW95_04005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOV21826.1"
                     /translation="MHTKNIIKKKREVKFEIINNQKKAKINLDQIKEKTEKLSKILSG
                     PPYNISIVFVSNRKIIKLNKKFLGRPFPTDVMSFKISKNHGEIIISIEKAKENSVIYN
                     YTNEEEILYLIIHGFLHLKGYRDYKKEEFENMKKFQDQIFKKLQNTR"
     gene            complement(7803..8780)
                     /locus_tag="PLW95_04010"
     CDS             complement(7803..8780)
                     /locus_tag="PLW95_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOV21827.1"
                     /translation="MEEIKEINFSNESTGSFGQAQIIYGQMDKNLKLLEKLLDVEIIG
                     RGNSVKIKGEKEKVEKAIEIFEILKKYVRNKKVIEKEEILKLIGEKAEKQEEKKDDFT
                     IHLNSKKKIVVPKSENQKEYIKSIKNYDLVIGIGPAGTGKTYLAVACGIEGVKEGKYQ
                     RLVLTRPALEAGERLGFLPGDLEEKIKPYLQPVYDALYDIMKYEELKRWTERKIIEVV
                     PLAYMRGRSLSNSFIILDEAQNTTFEQMKMFLTRLGLNSKAVITGDITQIDIPLTSLT
                     SGLVEIQTILSNIKGIKFVYFSHEDVVRHLLVKDIINAYEKHYKEKKRS"
     gene            complement(8781..9449)
                     /locus_tag="PLW95_04015"
     CDS             complement(8781..9449)
                     /locus_tag="PLW95_04015"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOV21828.1"
                     /translation="MRKFYLILILFLLTGCAGVQKKQEKEPTRDEIIDMKLKQTLAIA
                     ETSLEISKKSEEMAKQALETSNQANSTSDKALEAANKAIAAANEARKFTEEETKKAID
                     AANKAIEYADQTANKAIKAANDAIDAANKSSDKSISVANQTIAEINRLRATMVMKPEE
                     EPIIMEEPKTQNTYTIKKGDTLSEIAKKFYGNSSKWQQIYEKNKKVIKNPNVLIPGTN
                     IIIP"
     gene            complement(9612..11384)
                     /gene="aspS"
                     /locus_tag="PLW95_04020"
     CDS             complement(9612..11384)
                     /gene="aspS"
                     /locus_tag="PLW95_04020"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOV21829.1"
                     /translation="MLRTYTCGQLNIKNLGEEVILCGWVNSFRDHGGITFIDLRDRYG
                     ITQILCSPEVLNKDIWEKIKEIREEWVIKIEGDVVKRPEETINPKIPTGEIEIKLKNL
                     TILNKCKALPFEVSEYIEIGDNIRLKYRYIDLRRQKIKENLIKRSFFANNIRQFLVND
                     GFIEIETPVLTKSTPEGARDFIVPSRLNPGKFYALPQSPQLFKQILMIGGMDKYFQIA
                     RCFRDEDMRADRQPEFTQIDIEMSFIEEKDIMGITEKMLKHSIEKTFGIELKVPFPII
                     TYEEAMSVYGTDKPDLKDEMKFFNLTEIFKGSGLKIFEKVIEEGGIIKGFFVKKGENI
                     SLKDIDNFTNFVKEKGCSGLGWIRFKKDEIQSPFKKFLSEEMLKKLKSIYPESENTLL
                     FFLAGEKRNTEEMLFQLKENIKEKIDDKKKKCLDFVWVVDFPLFEYNSDEKRIQPVHH
                     PFTSPRQDDISLLEKEPLKVKSLAYDLVLNGIEIGGGSIRIHKKDLQEKIFKIIGIEE
                     NIYNERFGFLLQALDFGAPPHGGIAFGIDRLIMILVGEESIRETIAFPKTQKGVCPLT
                     QAPSDVDPQLLKENKIHIDIETDE"
     gene            complement(11378..12622)
                     /gene="hisS"
                     /locus_tag="PLW95_04025"
     CDS             complement(11378..12622)
                     /gene="hisS"
                     /locus_tag="PLW95_04025"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOV21830.1"
                     /translation="MGKYKRVRGMHDILPDESKKWNLFEKEGRQISELFGFEEIKVPV
                     VEEKGLFEKGVGEETDIISKEIYCFKDRKGRDIALRPEGTTPVVRAYIENQIYLKEKI
                     SRLYYYGPMFRYDRPQKDRYRQFYHFGVELFGGGNAYFDGEIINLLSSIFKKVNIENY
                     YFSINSLGCLNCKIMYSEKLKNYFQNFKEQICDDCKVRLEKNPLRVLDCKNRNCKEIS
                     KNAPSVLDILCDDCKIHFEKVIEYLNDININFKIDKNLVRGLDYYTKTVFEAFVENGE
                     SAVAGGGRYDNLVQQLGGPNIPAVGFAIGIERIIPYIKQVKLEEPIYCIFIGEKAKLW
                     GVKNLSILREHNIKIVFDFEDRDLSSHLKILNREKKKWCIIIGENEIQNNQIILKDME
                     NGTQFLINEKDIVNEIKEKIKC"
     gene            12724..12990
                     /gene="rpsO"
                     /locus_tag="PLW95_04030"
     CDS             12724..12990
                     /gene="rpsO"
                     /locus_tag="PLW95_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOV21831.1"
                     /translation="MLDTEEKIKLVKEYGRHEKDTGSPEVQIALLTNRIEALSKHFEK
                     HKKDTNSRRGFLKLIGRRRRLLRYLQMTDPEKYQEVIKKLNIRR"
     gene            12993..15065
                     /locus_tag="PLW95_04035"
     CDS             12993..15065
                     /locus_tag="PLW95_04035"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOV21832.1"
                     /translation="MEKKITIPCGNTPIIIELNKVAKQSNGSSFVRCGDTVVLTTVVC
                     AEEEKEDVDFFPLVCDYREQTSAAGKIPGGFFKREGKPTEREVLISRLIDRSIRPLFP
                     SDYYRDIQVVSFVLSADSENDTDILSIIGAAFALSSSNIDIKKNIFGVKVGMIDNKFV
                     INPTISQLEKSPLSLIIVGTEESVIMLEGQGNQISENDFLNAVEFGMENIKKFVKYVD
                     EVKGEKEEIKKNNFDETIDIENISSFLDNKLKDIFNYPEKKSRSLFYKSIEGELLEKY
                     PEEQKKEVKKIFECLLREKVRKIILETGKRLDGRGKEEIRPIECEVGLLPRNHGSALF
                     TRGQTQCLASLTLGTSADEQIIDGLYEETTKRFMLHYNFPPFSVGEVSPLRAPSRREI
                     GHGNLAERSLLPLIPDEKTFPYTIRIVANILESNGSSSMATVCAGSLALMDAGVPFEK
                     HIGGVALGMIKEGDKHIILTDIQGEEDHFGDLDLKIAGSQDGITGFQMDVKTDEFSLD
                     ILKQAVLQSRKARLEILEKMYKTISAPRENISPYAPKIYNISVSPDKIGLVIGSGGKT
                     IKKIIEETGADIDIQDDGNIRIYSSDINACKKAAAEIKKITKGPEVGDVFDGVVTKVL
                     PFGAIVKISSNQEGLVHISEIESYRIKKVDDVLKVGSKVRVKVIGVDEQGRVVLSRKQ
                     ALKDKKEK"
     gene            complement(15087..16847)
                     /locus_tag="PLW95_04040"
     CDS             complement(15087..16847)
                     /locus_tag="PLW95_04040"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOV21833.1"
                     /translation="MEEKTNFIIEEITIEEEKEKRIFKNFKIENNIYYSSDKNYCLSF
                     NFKNEGPFRKMNFKIKAKDRFVKKIVLKVKEEKENKFLNKESKVMVLPFDLSLPGGLF
                     KLDKKNLPVDSHICILIVNEKNSIIFGLASLPEDVCIFRIDDNYEFKIILDMNRYIKI
                     EEISIIYGQNNNPFDLIENYGTYLSKFGKKEAEIPIGWNSWDYYSCAITMDDLKKEMK
                     AIKNSALKDKVKYIVIDNGWEEEWGNWIPNRKFPSDLKEIADEIKKYGFIPGIWTAPF
                     LSSIYTTLARYRQELFVPSYIVKLYGPMVIFDLTLPEVHKFLFETFRKMKESGFSFFK
                     IDFLNQPLNIPSCFKDNTKGKIGAIREGIKTIRQAIGEESHLLACGAPLESVIGLADS
                     TRITEDIHNFWGHIKRNAIQISSQYWMNKKLWINDPDFAIIRCNETTDDKHLNRIYVK
                     RKFISENDYWLSGDECNLTELKTYLSLIYLSGGSIFLSDSIYRLNKNGIKILEKIIYP
                     LNNSAIPIDLFYNKPPRIWMNKKEKIIGIFNWEDEIMDFAEISKNMDIPEEGIEFWTG
                     KRTKLKNISLQKHSSILLEI"
     gene            17097..18044
                     /locus_tag="PLW95_04045"
     CDS             17097..18044
                     /locus_tag="PLW95_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOV21834.1"
                     /translation="MERIVFGPVPSRRLGQSLGINNIPPKICSYSCVYCQVGKTKNFS
                     IERENFYKVKEIVENVEKRLNEIRKRGEKVDYLSFVPDGEPTLDVNLGEEISQLKKFK
                     IKIAVITNSSLLWKDDVRKDIGKADWVSCKIDAVSENIWEKVNRPITGLDVKVIKKGI
                     VLFSNEYNGKLVTETMLVKGINDSKEEIEKISEFLKKVKPDICYISVPTRPPAEKWVG
                     VPEAENINIAYQIFKKKDLNVEILTGIGGNTFGFTGKIKEDILGTTSVHPMTEQQIEK
                     LLEKSGEKWEVVENLLKEGFLREVEFDGRKYFLRNFQKE"
     gene            complement(18095..18700)
                     /locus_tag="PLW95_04050"
     CDS             complement(18095..18700)
                     /locus_tag="PLW95_04050"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOV21835.1"
                     /translation="MVKKFIATFIFFITLFFSFPIYSIQGNEIFETKITPINNREYFP
                     SVLDKIKNAKKSIYIIMYLASYYPEYPESPSNQIINALINKKKENIKIEIILEQSDSK
                     YDEHLKTENLKTGSILAKNGITVHLDSPEIKTHSKLLIIDEKYVIIGSTNWSYSALEK
                     NNETSVIIESPKCAEYYIGYFEKIKKECPTKILPHFPSNSQ"
     gene            18852..19064
                     /locus_tag="PLW95_04055"
     CDS             18852..19064
                     /locus_tag="PLW95_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21836.1"
                     /translation="MGSGRGIKLGRKTISIIKQNLTFSLGFNFFMFIFASLAIINMIG
                     GALFHQVSSLTVILNSMRLLVISSEK"
     gene            complement(19080..19412)
                     /gene="cutA"
                     /locus_tag="PLW95_04060"
     CDS             complement(19080..19412)
                     /gene="cutA"
                     /locus_tag="PLW95_04060"
                     /inference="COORDINATES: protein motif:HMM:NF015077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HOV21837.1"
                     /translation="MGKNNFIQIITTFERKKDAKLLANILLKKRVAVCIQIIENIESY
                     YWWEGKIEKGKEILLFIKTKEEMYKKIEKIIKENHPYKIPEIISFKIHKGSKEYLKWI
                     KEEIGNND"
     gene            complement(19432..19821)
                     /locus_tag="PLW95_04065"
     CDS             complement(19432..19821)
                     /locus_tag="PLW95_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008424307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HOV21838.1"
                     /translation="MKITSFLKGKTEILKRDTHALYLAYRDPRVPLYAKLFIVCIVGY
                     ILSPLDLIPEFIPVVGYLDDIVILSIGVYFSIKMIPEYVWKECKERAISEDISKKLRW
                     TAGTIIVLIWIFIIFVIIKFISGIIKK"
     gene            complement(19886..20557)
                     /locus_tag="PLW95_04070"
     CDS             complement(19886..20557)
                     /locus_tag="PLW95_04070"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HOV21839.1"
                     /translation="MIKNYIKIVFILIFLNSICMADQFKYKWSSYTTYFQQNIDERGN
                     NIETGAKYLDGKILFPRVIFSFNNEITEKISDGELGTASTLIGDKRVLGLGGGLCQVS
                     STLYAAVLYAGLSIFERKPHSKLVSYIPPGLDATVSIDEGIDLKFYNPYTCNLMIKSE
                     VEGNSLKITILGTKPKVREIKVYVAKTIKQEQFLLTTTTRTVFSSGKELFSEIVSRDK
                     YLIPE"
     gene            complement(20554..25677)
                     /locus_tag="PLW95_04075"
     CDS             complement(20554..25677)
                     /locus_tag="PLW95_04075"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOV21840.1"
                     /translation="MKKFFYFFIFVFSFPCFCDTITGIIQTKEKKAISSANVSIFNIP
                     QTGKTPLKTVISDSNGKFKIDNLETGYYKIVVQKKGFCDSTLGKIEVNQAKFGVVKYE
                     IIMSIPGAISGFVYDQEGKPISGAKVFISKDIYTLTNSKGFYKLDGLNPGTKYIYVQA
                     DGFVKEHKNTVVEEGKEAGGINFSLPYAGSIKGIIVDSETEKPIDNVDISCSGTSFCS
                     TKTNKNGYFYIDGLKPGYYSLYAYRQGYEDIRIEKKFDLKPKETVDAGKIQMKIRAKY
                     FHLASKEWLFTPEEKVKVYFNAFRIKSATIDVYEIDLLNEINKTKLRDISINEILMSS
                     DLSGKSPVLSKKFEITYPTPLTELYDRNFLLDQFPEGAYVFALKPEGFAEIKGWFFVS
                     DIGFISKISNKKTNITLFSLTKGDILPDIQIFLFDNLWNLKATTTTDSNGQCNFESFN
                     KFIAVKGNSFAFSDTCNSIYGGTYENKTVYAYTDRPVYRPGQVVNFKAIPRIDMGDKY
                     SVAEFSSCEIKVKLPDETVVYETQIIPGTTNSIYGSYTLPEEPPLGTYKIEFMTIGKN
                     ELSGYCNFKVLEYRKPEFSIDIKTDKNVYLPGEKIKVLITAKYYFGTPVKNTDVLWAI
                     YSRQIWENDNDDYEYGEEYGGWWKGGLVCSGKGTTDENGISLIEIPTQLSYEIKQIFT
                     IEVRMTDESRREIIQSTKIVIVPGSFEIAITTSKYIYSPEEEIPVYLSIKYYDGREFK
                     GKKQLKLIVSQEKYDKNKRRWSYNNIISKNVILNNAKTTFNIKPGITGYIKIIVQGID
                     EYNNIISATKYVWITGRDYYSQWYGKKELEIVSDKKKYKPGETAKILINSSYSDLTIL
                     YTIEGYEIFENRVIKMNGNSVLLELPIKYEFIPNIYITICAVRNKSFLYSSKSLVVSC
                     DDKFLNVEITSDKPKYQPGEKAKYLIKIKDNTGNPVSAEFSMGVVDEAIYSISNELVP
                     EIEDFFYGLKGNNVITGYSFYRWYYGGAGKDFAQQDIRKNFKDTAFWLPLALTDENGI
                     GKIEVELPDNLTTWRTTIRSVTDDTKVGTGKNNITTAKPLMANLITPRFFVENDRLLI
                     SGVIHNYTGSQQKIYTKIHAEGLDILDETEKIIELPSGKSARVDWKVKVNSVKQVIIT
                     LYAKSSEFSDGMELKIPVFLYGTDERYVFAGQCDDVRVDTFYLPIGTIPSSIDIRSYI
                     YPSLVSGMFTSLEALSKYPYGCIEQTLNGFLPAIQVADTLTKIKSYDLYFLASDRIKF
                     EQMLKELPKKVSDGLIKLYNYQNEDGGWGWWQNDISNPYITGYVIFGLTHAKKSGYIV
                     NEDRYQRGKECLKNMLNTVNDYNQKEFILYVLTYTGENSLPVVEEIYNNRDKLNSYTI
                     AQLCLILKERGDTRANTLLDELCTKLVKPTTYLNYWAVEDGNYSWIHNNIEATAWGLC
                     AILAINPKREEIPFIIRYLVMKKQGGLWMSTKDTAICLFAFTNYLMITDELSPDYTFI
                     LSVNKKNIAEEKIDRESIKKFMTTIDINTENLSIGKINSIEIEKKGQGNLYYSNVIKF
                     SVKDISIPPFDGGFKILRRYTEIKSRETIDKEGNRIYVYDDINREVKSGEKIRVEIKI
                     SGGEKYEYVMIEDPIPSGCEVVNELSEDNWWYCRREIRDEKIVFFTTSWGAKEKTIVY
                     YLRAETPGSYNILPTKAQLMYLPEIWGHSQGNNLIIKEK"
     gene            complement(25674..26288)
                     /locus_tag="PLW95_04080"
     CDS             complement(25674..26288)
                     /locus_tag="PLW95_04080"
                     /inference="COORDINATES: protein motif:HMM:NF013988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMMECR1 family protein"
                     /protein_id="HOV21841.1"
                     /translation="MYNKIKRKLLNYYSKISRNFLVLCLAVNIFFPSVFEEKAIFICR
                     KTIENYITGYSEIIPENLPEIFNKPIPVFVSLKKGNQTRGCSGSFKTETGTLAGDLIK
                     FSIIAATEDYRYRPVDLQELKDIKIQITIPEIPVEISSISFYNPENEGLIVKKSGKQG
                     LVLPKEAKTGEYALKMCLRNAGINDEQGITIFKFKAKVFIEGEK"
     gene            26599..27045
                     /gene="nrdR"
                     /locus_tag="PLW95_04085"
     CDS             26599..27045
                     /gene="nrdR"
                     /locus_tag="PLW95_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOV21842.1"
                     /translation="MKCPYCSFLKDKVIDTREINNGLQVRRRRECLNCRKRFTTYEEI
                     EMKPIIVIKKDGRREKFNRDKIFSGIQRACEKRPISTEEIEKVIIEIEQELRQKGERE
                     IPSKEIGKIVIKKLKKLDKIAYIRFASVYRDFQDVSQFKKEIDKII"
     gene            27056..29146
                     /locus_tag="PLW95_04090"
     CDS             27056..29146
                     /locus_tag="PLW95_04090"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HOV21843.1"
                     /translation="MFEKIKKRNGEIVNFDASKITNAILKAGQATGEFGFDIAKKLTL
                     RVLDIAINTIGEKIPNVEEIQDIVEEVLISSPYKKTAKAYIIYREQHAKIREITTKAS
                     LDLVDQYLTKIDWRVNENSNMSFSLQGLNNYISSEITKTYWLNNIYPPEIRNAYINGD
                     FHIHDLGCLSVYCVGWDLQDLLTVGFKGAAGKVESNPAKHFRSALGQIVNFFYTLQGE
                     AAGAQALSNFDTLLAPFVKNDELRYKDVKQAIQEFLFNVNVPTRTGFQTPFVNLTFDL
                     VVPEIYKNQPVIIGGEFKNQTYSEFQEEMDMINRAFLEVMLEGDAKGRPFTFPIPTYN
                     ITKDFDWDNSNIKLLWEITGKYGIPYFSNFINSDMSPDDVRSMCCRLRLDKKELRKRG
                     GGLFGANPQTGSIGVVTIDLPRIGYLSKTKEEFKQRLERLMELARESLEIKRKVLEKF
                     TDEGLYPYSKFYLRSVKERTKKYWTNHFSTIGIIGMNEACLNLFGKDIGTQEGRDFAI
                     EIMNFIREKLIKFQEETGNLYNLEATPAEGTSYRLAKIDKTKYPDIIVANEESYKKGA
                     EPFYTNSTQLPVNYTDDIFLAFELQDALQTLYTGGTVFHIFIGEKIHSANGIKSLVKT
                     ICENFHLPYFTITPTFSICPNDGYISGEHFECPRCKSECEVYSRVVGYLRPISQWNKG
                     KQEEFKIRKTFSVK"
     gene            29143..29832
                     /locus_tag="PLW95_04095"
     CDS             29143..29832
                     /locus_tag="PLW95_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012543843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOV21844.1"
                     /translation="MKIGGLQKLSLIEYDGILSCVVFTIGCNFRCPYCHNPELVLPEK
                     FPVEIPLEDFFQFLKQRQKYLKGVCITGGEPTIQTDLPVFIKEIKELGFSVKLDTNGS
                     NPEMLEDLIGKGNIDYIALDVKGPLEKYNEIVKVEVDTEKILKSIYLIKNSAIPYEFR
                     TTVVKSQLSFDDFKKIGKTISGCKNYYLQKFIPSKCVDPSFINEPTYSDDELEKIKEI
                     MLNYVENCYIR"
BASE COUNT        10269 a         4368 c         4243 g        11105 t
ORIGIN      
        1 ctggccctgg gggagggccg ggtcccacta ttcctttctt ccttcttttt tccttttttg
       61 taacattttc atttggcaat ttgtaacatt ttcacttggt aataacaatt cattgacaaa
      121 atattttaaa tgggtataat tttgtaaatg gctataataa gtataggaaa taaaataaaa
      181 aaattaagag aaaatagagg aatacattta agaactcttg cagaaagggt tggggtttca
      241 ccttctttta tttcccaact tgaaaataat aaaacatctc cgtcgcttgt aacattaaaa
      301 aatatagcag atgcacttgg aacaacagta tcttttttaa ttggagatga aaaaaaagaa
      361 acagaaacac ctgtgatgaa gaaggaagaa tggatttttc aacaaatggg agaaggtatt
      421 aaaatgtttt atctcacttt tcccgaccct tataaacaaa ttgaaccact tttgtttgta
      481 atggaaaaag gggcaagttc tggtgaaaac gtttatcaac attttggaca agagtttgtg
      541 atggttttaa atggaaaact tgaagttaca ttggcagaaa agaaatatgt aattcatagt
      601 ggagatacta tttattttaa ttcatatatt ccacataagt ttaaaaatgt tcataaggga
      661 gttacaaaag ttgtatgggt agataatcca cccacatttt aatttttttt gactttttgt
      721 taagtatata ttaaaatatt aaatgaaaat taaaaggagg taaaaaatgg aagaattatt
      781 gattagtaaa acaaagggtt tgacagaaga gatagagtat tttgctaatg agattatagg
      841 catgaaagat acggataaga tgcttaaaag aattggcgat gaaatgaaat atcagaatta
      901 tgattatgtt gaggaaaaag aaaaaatggt ttgtggggaa ataagcggaa ttaaaaagga
      961 aggtagtttt tacttaatat ctgatttagg acagggaatt gaagcaaatg gtattcataa
     1021 tggagttatt acatccattt ttgttggggc tcttattaag agatgtttat tgcctttaag
     1081 gaatgatttg aatgttatta tttctgatga ggaaattgaa aaattatttc aaaaaatttc
     1141 ctataaaggt gcaaaaggaa taataatttg caagccaaca aattatcaaa tttataaaat
     1201 caacaaagaa aaagataatt atgagaagtt aaatggttat tattcttttg ttcacacatt
     1261 ggtttctact ctggaagaag aaagatatga gacagttttg agagaaaaag aaatgccagt
     1321 gacaacaaca attcctattg ttgaatttgg tggagatgat acttttgaga tagggaaaat
     1381 agaaaaaaca atttatataa ttacaaaggt agtaataaga tgtgttggat ttcctttgtt
     1441 tggatggtcc gcagatgaaa tataaaacta aaatattggt tttaaattgt gggagttctt
     1501 cacttaaatt taaggttatt tcttttcctg aggagataga gattgtttca ggcgaagcag
     1561 aaaaagttgg ggttaaaagt actacctcat caagaattaa aatgagaata gaaaatgata
     1621 cttatgagat taaagaaaat ttaccggacc atttttctgc gtttaaaaca gttgtaaatt
     1681 atcttcaggg gaaaattaac tttgatataa ttggacatag gtatgttcac cctggtaatg
     1741 aaaaaaagca ttctttaatt ataaatgagg agtcaatcag atatttagaa agcacactcc
     1801 attttgctcc tcttcataat ccagtttctt tttcaatgat aaaattttct caagaaattt
     1861 ttcctgatat acctcaatat gctgtttttg atatatcttt ccataataca attcaaccag
     1921 aattttctac atatgcttta ccattagaac tctcacaaaa atataatata aaaagatatg
     1981 ggtttcatgg gatttcccat cagtatgtta tggaagaggc attgaaattc ttaaatgtag
     2041 aaaaggaaaa agtaaaaatt ataagtgcac atttaggaac aggtggttca agtttatgtg
     2101 ctattaaaaa tggagaatca atacacaata ctatgggttt tactcctctt gaaggattaa
     2161 taatgaatac tcgttcaggt gatatagaca taggaattgt tctttacata atgtttttaa
     2221 aaaagttcac tccagaccag atggataaca ttttaaataa acaaagcgga ttggcaggta
     2281 tttataaaga aacatcagat ttaagagaaa ttgcaagtcg tgttaatgat gatggaaggg
     2341 ctgaaaaagc atttgatatg tatgtcagaa gaataaaaaa atatattggc ttttattttt
     2401 taattttaga aggttttaat gttttaattt ttacagatac tcttggagtg gaagttcctt
     2461 tattaagaga aaagatatgc gatgaaatgg attttcttgg tattaaatta gataaggaaa
     2521 agaataaaaa ttctactgat tattgtttaa aagatatttc agcagatggg tcaaaagtta
     2581 aaattcttgt tgttccaaca aatgaagaat tgatgattgc aagagaaatt tataggggaa
     2641 taaaatgata cttttattaa atccgtctat atataaaatt gaaattactg gttctgatga
     2701 taaaagtaat tcatatgaaa ttgaggagat aaaaaaactt tctttataca aagacaaaac
     2761 tgaggcaatt agtatcagaa ttctctgggg tggtgaattt ttaagaaata agggacttat
     2821 tgataataac tttatagaaa aatataaagg actaataact ttttttccag tatatgtccc
     2881 gagaatagag aaaattctta aatatcttct tgaaaatttt caagatatac ccattattcc
     2941 attttttgaa gataaatttt ttcttaattt accggttgaa gaaaaatatt acggaattaa
     3001 ttctttcaga atagggagaa tgaaaattcg taaaagaggt tttcatggaa tttttcatga
     3061 atttgtgtct gaaaaatttt cttcatataa aaaaattata tcaattgtct tagataaaaa
     3121 aacaacaatc tgtggaatta aaaataggaa gccacaattg ataagtattg gatttactcc
     3181 tcttgagggt ataatgggga aaacaagttc aggtgactta gaccctggaa ttgtttttta
     3241 tatgatgaaa gaattaggat attcaatttt tgaagtagat aacattttaa agaaaaaaag
     3301 tggtttctat gggttaacaa gatacaatag agatataaac gaaatttata aaaattttta
     3361 taaaaaggat gaaaaagtta attttgcctt taaaatttat aaaaagcaga taataaaata
     3421 tataggagaa gcaatttctg ttttggatgg agttgatgca attgtgtttt ctggcgaata
     3481 tgtaaaagaa atgatatcat ttatatatga aataatttct gaaatttcat ttattggaat
     3541 taaattggga aaaattagta aattttcaga taatataggt ataatatcta tagaaaaaat
     3601 tccagtattt ttaaactttt atacagataa agaaattatt ttttatcaga cagaaaaagt
     3661 tttaaaaaat tttgtttagc accccctttt tcctcatgta gaaccctgtt ctcaaatgag
     3721 aacagggttc tacgctatca actgaaaacc caaatttaaa atctgttaaa atagaattgt
     3781 tttttatgga aaagataaaa tcgggggaga gggatttgaa ccctcgacct cagcgtcccg
     3841 aacgctgcgc gctaaaccgg actgcgctac cccccgtttt ctccagacct ggtatggaga
     3901 aaaatgtgcc cagccagatt tgaactggca acttccaggt ccgcagcctg gcgttctatc
     3961 cagttgaact atgggcactt tttatttata aagataattt ccataaatat caatcccaac
     4021 tattgcagga aaattttcaa cttctacttt ataaatacct tctgccccta attcttcaaa
     4081 agcaataagt tctatttttc ttacaaattt tgataaatat gctccacagc caccaaaggt
     4141 aattaaatat attgctttat attttctaca tgcatttata acttctttgt ttctgctccc
     4201 ttttcccatt gttcctttaa gtcctatttt atataggaga ggagtaaaag tatccattct
     4261 actacttgtt gtcggaccaa aagaaccgac tattttcccc ggtggtgcag gagttggacc
     4321 aacataataa ataagattgt gattaagagt aaatggtaaa tttttatttt cttctattaa
     4381 cttaaaaatt tttttatgtg ctgcatctct acaggtataa aatattccat taagaaatat
     4441 aaaatcccca atttttaagt tcgctaattt tttttcatct actggaaaga atattttttt
     4501 cataattcaa gtttctcaac tctatgagcc cagcatgaaa tacaaacact aactggcagt
     4561 ccagcaatat gtgttggatg tgtttcaatc tttaaatcaa gggctgttgt ttttccacca
     4621 aaaccgcctg cccctatttt tagtttattt atttttttca taatttctaa ttcccaattt
     4681 ctatacttac ttttttctcc aatttttaaa agagatattt ttgccagtaa aggagacatt
     4741 tcaaaagtcc cgccaattcc aactccaaca aaaattggag gacaaggcaa agagcctgcc
     4801 tgttttattg tatcaactat aaaattttca attccttctt ttccatcagc aacatttaac
     4861 attttcatct tacatgtatt ttcagaacca aatccctttg ccattatagt aatttttaat
     4921 ttattttctt tgccttcaaa aaaatgtatt acacaaggag tattatatcc agtaaaattc
     4981 ctatcaattg gctcaacaac agaatttctc aaatatcctt ttttactccc atcttcaata
     5041 ccaaaattta ttatatctat taaagaagga aatggtcctt tttcaatttt tacattagaa
     5101 ccaatttcaa caaaaactac aggatatcct gtatcctgac ataaaggcaa tttttctttt
     5161 tttgatattt ttatattttc aaggactatt ttaagatatt tttttgaaag tccatttctc
     5221 tcctggtaat atgctttttt taaatattcc tcaatatttt ttgaaactcc atagttaata
     5281 tctattatta aatttgatac ttcttttata atctgtttat atgagatttt tttcattttc
     5341 ccaccatatt ttgtcaatat cgctatggaa atcaagagtt gtgttaatat aaaaatttaa
     5401 cttatttaaa atatctttat gaatactttt tattattttt attttatcaa agtccagagt
     5461 atcaaacatc ctcattattt taagaacagg aaatgaaatt ctgactggat ttatatcttt
     5521 tttattacaa tttctacaaa taattccacc taatttcccg ctaaaaaagt acattttatc
     5581 ttctttatta caggaaatac acttttctaa atgaaaacca tatccaagag attttatgaa
     5641 ttttgtttta aaaaaagtta aaacaatttc aggattactt gctttttcta aataattacc
     5701 aatttctaat gttaattgta aaattttgtt atcctgtaaa ttttctggtg taaaaatatc
     5761 taagaggtta agtatataga accagacatt tgaattcttt ttctctttta tttcaaaatt
     5821 gttcaacagg ctcggagaac taaaaagatt taattcacct ttctttttgt aaatcaatgt
     5881 aaaaatatga gagccgggct gaaatgttaa aggtgctatt ttgctttttt cacttcttac
     5941 tcctttcaaa acacctttaa tttttcccat gttttcagta taaatactta gtattacact
     6001 tgtttctcta aaatcaaatt tttttaaaac aattccttca ctctttattg gtggcatttt
     6061 cagcaaatcc cgcagaaatc ataattttaa atccttcttc aactgagata ttaagttttt
     6121 caaccatttt ttctggaaca agaacataaa aaccagtagt tatatttggg acagttggaa
     6181 caaatatact taaaaacttt tcttctttta tcagttgtga ttcgccagta ataaaaccaa
     6241 ctgtataact tccatcaaaa aattttattt ttgcaactcc tttaaaaatt tgctcttttt
     6301 tacttgtaac actctgaaca atacttttaa taaaattata aattctattt aaaacaggga
     6361 ctttttcaaa aataacttca attcttctta aaagtttctt tccaatgaaa ttttgagtaa
     6421 gaaagccaat aaaaagaatg attaaaataa gcgagaaaaa gccaattcct ggggtatata
     6481 tatgcgggga aaattttttt aataaattgc ctataattcc atctaaaaag acaattattt
     6541 tatataaaat ccatattgaa agagaaattg gtattataaa aacaagccct gaaataaaat
     6601 attttcttag ttttcccatt ttatctccag aacacttgaa aaatcaatat tgttaaaaga
     6661 atcccaataa ttcctcctat aattacttca tttaccttat gtattttttt acttattcta
     6721 ctttctgcaa cccatattgc taaaattaac acaagaaaag atattatagg gaattcttta
     6781 aaagttaaaa tagaaattgt tgtccagatg ctaaaagcaa tacctgagtg aatacttggc
     6841 attcctcctg aaagagaaaa ttccttttca attgaaaatt ttagtaaaat agcaagagaa
     6901 atgacaatta caagtgctat aaaagtaata tgccattgag aatttgcaat attttcaaat
     6961 atatttctcc aaccagatgg aaaatatttc acaaaaatta aatatccacc aataaaagaa
     7021 aaaaaggaag ctacaagaac agaggaagca gcaatgtctt ttattttctt tatttcctta
     7081 tgattttcta ttgtaaacag atttgaaaaa ttctcaattg atgtatttat aatttctgct
     7141 ccaaaaacaa gtgaaataaa tataagaata attaaaaatt cactaagtgg gattttcaaa
     7201 aataaacttc ctgttataac acaaattgca atgaaaatat gtattttaaa atttctctct
     7261 gtttttaaag cataaaaaaa tccttcaatt gctgaattga aactacttaa aatatcatga
     7321 ttttttttat tctctttcat tattcccttt tataaaaggc acgcaaattt ttgtgtttat
     7381 tattatatca tttttacctg gtgttttgaa gttttttaaa tatttgatcc tggaattttt
     7441 tcatattctc aaattcctct tttttgtagt ccctgtaccc ttttaaatgt aaaaatccat
     7501 gaattataag atataaaatt tcttcttcat ttgtgtaatt atatataaca gagttttctt
     7561 ttgccttttc tattgaaata attatttcac catgattttt tgaaatttta aatgacataa
     7621 catctgttgg aaagggtcta ccaagaaatt ttttatttag tttaattatt tttctattac
     7681 tgacaaaaac aatagatatg ttatatggag gccctgataa aatttttgat aatttctctg
     7741 ttttttcttt aatctggtca agatttatct ttgctttttt ttgattgttt ataatctcaa
     7801 atttaacttc tctttttttc tttataatgt ttttcgtatg catttattat atctttaaca
     7861 agtaagtgtc ttacaacatc ttcatgagag aagtagacaa atttaattcc ctttatatta
     7921 gaaaggattg tttggatttc tactaaccct gatgtcaggg aagttaatgg aatatcaatt
     7981 tgtgtaatat ctcctgttat aactgccttt gaattaagcc caaggcgagt aagaaacatt
     8041 ttcatctgtt caaaagttgt attttgtgct tcatctaaaa ttataaatga attacttaaa
     8101 cttctacctc gcatataagc aagtggaact acttctatta tttttctctc tgtccatctt
     8161 tttaattctt catatttcat aatgtcataa agagcatcat atactggctg taaatatggt
     8221 tttatttttt cttcaaggtc accaggtaaa aaacccaatc tttctcctgc ttcaagggct
     8281 ggtcttgtaa gaacaagtcg ttgatatttt ccttccttta caccttcaat tccacatgca
     8341 acagcaagat atgtttttcc agttcctgct ggacctattc caatcacaag gtcataattc
     8401 tttatacttt ttatatattc tttttgattt tcactttttg gaacaactat ttttttcttt
     8461 gaattcaaat gaatagtaaa gtcatctttt ttttcttctt gtttctctgc tttttcacca
     8521 attagtttta gaatttcttc tttttctata acttttttgt ttctaacata ctttttaagt
     8581 atttcaaaaa tttcaattgc tttttcaact ttttcttttt caccttttat tttgacagaa
     8641 ttaccacgcc caataatttc aacatcaagc aatttttcaa gtaatttaag atttttatcc
     8701 atttgcccat agattatctg ggcttgtccg aaggatcctg tagattcatt agagaaattt
     8761 atttctttta tttcttccat ttaaggaatg attatatttg tcccaggtat aagtacattt
     8821 ggatttttta taactttttt gtttttctca tatatttgtt gccatttgct tgaatttcca
     8881 taaaattttt ttgctatttc tgaaagagtg tctccctttt taatggtata tgtgttttga
     8941 gtttttggtt cttccattat aattggctct tcttctggct tcattaccat tgttgctctt
     9001 aatctattta tttctgctat tgtttgatta gcaactgaaa ttgatttatc gcttgattta
     9061 tttgctgcat ctattgcatc atttgctgct ttaattgcct tatttgctgt ctggtcagca
     9121 tattcaattg ccttatttgc tgcatcaatt gctttttttg tttcttcttc agtaaatttt
     9181 cttgcttcat ttgctgctgc aattgctttg tttgctgctt ctaatgcttt atcagatgtg
     9241 ctgtttgctt gattacttgt ttcaagggcc tgttttgcca tttcttctga ctttttactt
     9301 atttctaaac ttgtttctgc aattgcaagt gtctgtttaa gtttcatatc aataatttca
     9361 tctcttgtag gttctttttc ttgttttttt tgaacccctg cacaaccggt aagcagaaat
     9421 aaaattaaaa ttaaataaaa tttcctcata ttatctcctt tttaatatta ttgaatactt
     9481 tttatctttc agttaaatcc tttctcactt cccttttata aataaaaaat ggagaaattt
     9541 tttcttcttt tctcataact tactactttc tacttttcaa gcaacgcagt caattgaacg
     9601 cttacaatat atcattcatc tgtttctatg tcaatatgta ttttattttc tttaagaagt
     9661 tgtgggtcaa catctgaagg tgcctgtgta agagggcata cgcctttttg tgttttggga
     9721 aatgcaattg tttctcttat actttcttct ccaactaaaa tcataataag tcggtcaatc
     9781 ccaaaagcaa ttcctccatg aggtggtgcc ccaaaatcaa gtgcctgtaa taaaaatcca
     9841 aatctctcat tataaatatt ctcttcaatt ccgataattt tgaaaatttt ttcctgtaag
     9901 tcctttttat gaattcttat acttccgcca cctatttcaa ttccattcaa aacaaggtca
     9961 taagcaagag attttacttt caatggttct ttttcaagaa gagaaatatc atcttgtctt
    10021 ggagaggtaa acggatgatg aactggttga attctctttt catcagagtt atattcaaaa
    10081 agtggaaaat caactaccca gacaaaatca agacatttct tttttttatc atcaattttt
    10141 tcctttatat tttcttttaa ttgaaatagc atctcttcag tattcctttt ttctccagca
    10201 aggaaaaata acaatgtatt ttcactttct ggataaattg attttaattt tttgagcatt
    10261 tcttctgaaa gaaatttttt aaaaggactt tgtatttcat cttttttaaa ccttatccag
    10321 ccaagtccac tacatccctt ttctttaaca aaatttgtaa aattatcaat atcttttaat
    10381 gagatatttt cccccttttt aacaaaaaat ccttttataa ttcccccttc ctcaattact
    10441 ttctcaaaaa ttttgagccc acttccttta aatatttccg ttaagttaaa aaatttcatt
    10501 tcatctttca ggtctggttt atctgttcca taaacagaca ttgcttcttc ataagttatt
    10561 ataggaaatg gcacttttaa ttcaatcccg aatgtttttt caatggaatg tttaagcatt
    10621 ttttcagtta ttcccattat atctttttct tcaataaatg acatttcaat atctatttga
    10681 gtaaattctg gttgtctatc agcacgcata tcttcatctc tgaaacatct tgcaatttga
    10741 aaatatttat ccataccccc tatcataaga atttgtttaa agagttgagg ggactgcgga
    10801 agagcataaa attttccagg atttaatctt gatggaacaa taaagtccct tgctccttcg
    10861 ggagtgcttt tagtcaaaac aggtgtttca atttcaataa atccatcatt tacaagaaat
    10921 tgccttatat tatttgcaaa aaaagacctc tttataagat tttctttgat tttttgtctt
    10981 ctgaggtcaa tgtatctata tttcaggcga atgttatctc caatttctat gtattcggag
    11041 acctcaaaag gaagtgcctt gcatttgttt aaaattgtta aatttttaag ttttatttca
    11101 atttctcctg ttggaatttt aggatttatt gtttcttctg gccttttgac aacatcccct
    11161 tcaattttaa ttacccattc ttcccttatt tccttaattt tttcccatat atctttattt
    11221 agcacttctg gtgaacataa aatttgtgta ataccatatc tatctcttaa atcaataaat
    11281 gtaattcctc cgtggtccct aaatgagtta acccacccac ataatattac ttcttctcct
    11341 aaatttttaa tatttagttg tccacatgta taagttctta gcattttatt ttttccttta
    11401 tttcatttac tatgtctttt tcatttatta aaaattgcgt cccattttcc atatctttaa
    11461 gtattatttg attattttga atttcatttt ctccaattat tatacaccat tttttctttt
    11521 ctctattaag tatttttaaa tgtgaactta aatctctatc ctcaaagtca aaaacaattt
    11581 ttatattgtg ttctcttagg atacttaaat ttttaacacc ccataatttt gccttttccc
    11641 caataaaaat acaataaatt ggttcttcta atttaacctg ttttatatat ggaataattc
    11701 tttctattcc aattgcaaat ccaactgctg gtatattagg tcctcctaat tgttgaacaa
    11761 gattatcata cctgccacca cctgctactg cactttctcc attttcaaca aatgcctcaa
    11821 agacagtttt tgtatagtaa tcaagacctc ttacaagatt tttatcaatt ttaaaattga
    11881 tgtttatatc attaagatat tcaataacct tttcaaaatg tattttacaa tcatcacata
    11941 aaatatccaa tacagaaggg gcattttttg aaatttcctt acaatttcta tttttacaat
    12001 caagaactct taatggattt ttctccaatc ggactttgca atcatcacaa atttgttctt
    12061 tgaaattctg aaaataattt tttagttttt cagagtacat tattttacaa tttaaacatc
    12121 caagagaatt tatagaaaaa taataatttt ctatattaac ttttttaaaa atagatgaca
    12181 ataaatttat aatttctcca tcaaaatatg cgtttccacc accaaataat tctactccaa
    12241 aatgataaaa ctgcctatat ctatcttttt gaggcctatc atatctgaac ataggtccat
    12301 aatagtaaag tcgtgaaatt ttttctttta aatatatctg gttttcaata tatgctctta
    12361 caacaggggt agttccctct ggtcttaaag caatgtccct tccttttcta tctttgaaac
    12421 aatatatttc ttttgaaata atatctgttt cttcaccaac tcctttttca aaaagtcctt
    12481 tttcttcaac gacaggcact tttatctctt caaaaccaaa aagttcagat atctgtctac
    12541 cttctttctc aaataaattc catttcttac tttcatcagg caaaatatca tgcattccac
    12601 gaactctttt atattttccc attttttcct ttctcttatt tatgatataa tttaccacaa
    12661 aattttatgt tgacaaaaat atttattgtt gtataattat agagataagt aaaggagaaa
    12721 taaatgttag atacagaaga aaaaataaaa ttagtaaaag aatatggaag acatgaaaag
    12781 gatactgggt ccccagaagt tcaaattgct ttattaacaa ataggattga ggcgttaagc
    12841 aagcattttg agaaacataa aaaagataca aattcaagaa ggggcttttt aaaacttatt
    12901 ggaagaagaa ggcgtctttt gagatattta cagatgactg accctgaaaa ataccaagaa
    12961 gttatcaaaa aattgaatat tagaagataa aaatggaaaa gaaaattaca ataccttgtg
    13021 ggaatacccc aataataatt gaattaaata aagttgcaaa acaaagtaat ggttcatctt
    13081 ttgtaagatg tggtgatact gttgtcttaa caactgttgt ttgtgcagaa gaagaaaaag
    13141 aagatgttga tttttttcct cttgtatgtg attacagaga acaaacatcc gcagcaggta
    13201 aaattcctgg tggatttttc aaaagagaag ggaaaccaac agaaagagaa gttcttatta
    13261 gccgactgat agataggtca ataagaccgc ttttcccttc tgactattac agagatattc
    13321 aggttgtttc ttttgtccta tccgctgatt cagaaaatga taccgatatt ctttcaataa
    13381 ttggtgctgc atttgcatta agtagttcaa acatagacat taaaaaaaat atttttggtg
    13441 taaaagttgg tatgattgat aataaatttg ttattaatcc aacaatcagt cagttagaaa
    13501 aaagtcctct ttctttaata attgttggaa cagaggaatc agtaattatg ttagaaggac
    13561 agggcaatca aataagtgaa aatgattttt taaatgctgt tgagtttggg atggaaaata
    13621 ttaaaaaatt tgtgaaatat gtagatgaag taaaaggaga aaaagaggaa attaaaaaaa
    13681 ataattttga tgagacaata gatatagaaa atatttcttc ttttcttgat aataaattaa
    13741 aagacatatt taattaccct gaaaagaaaa gcagaagttt gttttataaa tccatagaag
    13801 gtgaactact tgaaaaatat ccagaagaac aaaagaaaga agtaaaaaaa atttttgaat
    13861 gcttattaag ggagaaagtc agaaaaataa tacttgagac aggaaaaagg ttagatggaa
    13921 gaggtaaaga ggaaattaga ccaatagaat gcgaagttgg actcctgcca agaaatcatg
    13981 gttctgccct ttttacaagg gggcaaacac aatgtttggc atctttgaca cttggaacaa
    14041 gtgcagatga acaaattatt gacgggcttt atgaagaaac aacaaaaaga tttatgctac
    14101 actataattt tcctcctttt tctgttggag aggtttctcc attaagagca ccttcaagaa
    14161 gagaaatcgg gcatggcaat ttagcggaaa ggtcacttct tccacttatt cctgatgaaa
    14221 aaacattccc atatacaata agaatagttg caaatatact tgaatcaaat gggtcttctt
    14281 caatggcaac tgtttgtgct ggttcccttg cgttaatgga tgcaggtgtg ccatttgaaa
    14341 aacatattgg aggagttgca ttgggtatga ttaaagaagg agataaacac ataattctaa
    14401 cagatattca gggagaagaa gaccattttg gagaccttga ccttaaaata gcaggaagtc
    14461 aggatggcat aactggtttt caaatggatg tgaagacaga tgaattttct cttgatattt
    14521 taaaacaggc tgtcttacaa tcaagaaagg cacgacttga aattcttgag aaaatgtata
    14581 aaacaatttc tgctcccaga gaaaatattt caccttatgc tccaaaaata tacaatatct
    14641 cagtttctcc tgataaaata gggcttgtaa ttggttcagg aggtaaaaca attaaaaaaa
    14701 ttattgaaga aacaggggca gatattgata tccaggatga cggaaatata agaatttact
    14761 catcagatat aaacgcatgt aaaaaagcag cagcagaaat aaaaaaaata acaaaaggac
    14821 ctgaggtggg agatgtattt gatggagttg taacaaaggt cttgccattt ggtgcaatag
    14881 ttaaaatttc ttcaaatcag gaagggcttg ttcatatttc agaaatagag tcgtatagaa
    14941 taaaaaaagt tgatgatgtt cttaaagttg gctcaaaagt tagagtaaaa gttattggag
    15001 ttgatgaaca gggaagggtt gttttaagta gaaaacaagc actcaaagat aaaaaagaaa
    15061 aatgattttt ctatcaccaa atattcttat atttctaaaa ggattgaaga atgtttttgt
    15121 aaagagatat tctttaattt agttcttttc cctgtccaga attctatacc ttcttctggt
    15181 atatccatat tcttacttat ttctgcaaaa tccataattt catcttccca gttaaaaata
    15241 ccaattattt tttctttttt attcatccat atacgaggtg gtttgttgta gaaaaggtct
    15301 atcggaattg cagaattatt aagtgggtaa attatttttt caagaatttt aattccattt
    15361 ttgtttaatc tataaataga atcgcttaaa aatattgagc caccagaaag atatataaga
    15421 gaaaggtatg ttttaagttc ggtaagatta cactcatctc cactaagcca gtaatcattt
    15481 tcagaaataa attttctttt tacataaatt ctatttaaat gcttatcatc tgttgtttca
    15541 ttacatctta ttattgcaaa atcagggtcg tttatccata attttttatt catccagtat
    15601 tgagaagata tttgtatggc atttctctta atatgtcccc aaaaattatg tatatcttct
    15661 gtaattcttg tactatctgc cagtccaatt acactttcta aaggagcacc acaggcaaga
    15721 agatgacttt cttctcctat tgcctgtctt attgttttaa ttccttccct gattgctcct
    15781 atttttcctt ttgtattatc tttaaaacat gatggaatat ttaatggctg atttagaaaa
    15841 tctattttga aaaatgaaaa cccagattct ttcatttttc taaatgtttc aaatagaaat
    15901 ttatgaactt ctggtaaagt taaatcaaaa ataaccatgg gtccataaag tttaactata
    15961 tatgaaggga caaataattc ctgtctatat cttgcaagtg ttgtatatat gctggataaa
    16021 aacggtgcag tccatattcc aggaataaaa ccatattttt ttatctcgtc tgctatctct
    16081 tttaaatctg atgggaattt acggttaggt atccaatttc cccactcttc ttcccatcca
    16141 ttatctatta ctatatattt aactttatcc tttaaggcag aattttttat tgccttcatt
    16201 tcttttttta aatcatccat tgtaattgca caactataat aatcccagga attccaccca
    16261 attggtattt ctgcctcttt cttcccgaat ttgcttaaat atgtcccata attttcaatt
    16321 aaatcaaatg gattattatt ttgtccatag ataatactta tttcttctat ctttatatat
    16381 ctattcatat ccagaataat tttaaattca taattatcat caatcctgaa aatacaaaca
    16441 tcttctggaa gtgatgcaag gccaaaaata atagagtttt tttcatttac aattaaaatg
    16501 caaatatgtg aatcaactgg taaatttttt ttatctaatt tgaatagacc accgggcaaa
    16561 gataaatcaa aaggtaatac cattactttt gattctttat ttaaaaattt attttctttt
    16621 tcttctttaa cttttagaac tatttttttg acaaatctat cttttgcttt tattttaaaa
    16681 ttcatctttc taaatgggcc ttcgttttta aagttaaatg ataaacaata atttttatca
    16741 gaggaataat aaatattatt ttctatttta aaattcttaa atatcctttt ttctttttct
    16801 tcttcaattg taatttcttc tattataaaa tttgttttct cttccatttt cctccattat
    16861 aaaatacttt tgaaattttt agttaattat ttatctaatt tctcttctta cctgttatat
    16921 tttaagtaat aaaagagaaa ttttgtaatt tatgataata tatttttaca ttttataaaa
    16981 gcattacaat ttttaagaaa tgccgagaac ttgaaataat aaggaaaaag aagtaaaatt
    17041 gtctaatatt aaggatgttt ttcttgtctt caaaaaagaa taaaggatat ataaaaatgg
    17101 aaagaattgt ttttggtcct gttccatcaa gacgattagg tcagagtttg ggaattaaca
    17161 atattcctcc gaaaatttgc agttattcat gtgtttattg tcaggttgga aaaacaaaga
    17221 atttttcaat tgaaagggaa aatttttata aagttaaaga aattgttgaa aatgtagaaa
    17281 agagattaaa tgaaataaga aaaagaggag agaaggttga ttacctttct tttgttcctg
    17341 atggagaacc aacacttgat gtaaatttag gagaagaaat ttctcaacta aaaaagttca
    17401 aaataaaaat tgcagttata acaaattctt ccttgttatg gaaagatgat gtaagaaagg
    17461 atattggaaa agcagattgg gtaagctgta aaattgatgc tgtaagtgaa aatatctggg
    17521 aaaaagttaa tagaccaatt actggacttg atgtgaaagt gataaaaaaa ggaatagttc
    17581 tattttcaaa tgaatataat ggtaaattgg taacagaaac aatgcttgta aaaggaataa
    17641 atgatagcaa agaagagata gaaaaaataa gtgaattttt aaagaaagta aaacctgata
    17701 tttgctatat ttcagttcct acaagacctc ccgctgaaaa atgggttggt gttccagaag
    17761 cagaaaatat aaacattgca tatcaaattt tcaaaaagaa ggacttaaat gttgagattt
    17821 taacaggaat tggtggcaat acatttggct ttacaggaaa aattaaagaa gatattttag
    17881 gaacaacatc tgttcatcct atgacagaac aacaaattga aaaactttta gaaaagtcag
    17941 gtgaaaaatg ggaagttgtt gaaaatcttt taaaagaagg ttttttgaga gaagttgaat
    18001 ttgatgggag aaagtatttt ttaagaaatt ttcaaaagga ataaaaataa gcgaaaatat
    18061 ccaggtgttc tcttcccgaa acttttaaat attatcattg agaatttgat ggaaagtggg
    18121 gaagaatttt agttgggcat tctttcttta tcttctcaaa atatccaata taatactctg
    18181 cacatttagg tgactcaatt attactgatg tttcattatt tttttcaaga gcagaatatg
    18241 accagttagt acttccaatt attacatatt tttcatcaat tattagaagt ttagaatgag
    18301 tttttatttc aggggaatct aaatgtacag taataccatt ttttgctaat atactacctg
    18361 tttttaaatt ttctgttttt aaatgttcat catattttga gtcagattgt tctaaaataa
    18421 tttcaatttt tatgttttct tttttcttat taataagtgc atttataatt tgatttgatg
    18481 gactttcagg atattctgga taataacttg ctaaatacat aattatataa attgattttt
    18541 tagcattttt tattttatca agaacagaag gaaaatattc tctattatta ataggagtga
    18601 ttttagtttc aaatatttca ttcccttgaa ttgaataaat aggaaaggag aaaaacaaag
    18661 ttataaaaaa gataaaagtt gctataaatt ttttcaccat tttatataac cgcgggggtt
    18721 aaatttacgc ggttagaata atttttacaa ctatattata tattatttac cgcaagtcat
    18781 caaaagggaa aaaagggtgg aggttttgcg gtctactttc ctccagacca agtatggagg
    18841 actgtcttgt tatggggtca ggtagaggaa taaaattggg aagaaaaaca atttctataa
    18901 ttaaacaaaa tttaactttt tcattaggat ttaatttttt tatgtttatt tttgcaagtt
    18961 tggcaattat aaatatgata gggggtgctt tatttcatca agttagttct ttaactgtta
    19021 tcttaaattc aatgagatta ttggtaattt cttctgaaaa ataaagtcat aaaatttttt
    19081 tagtcattat ttcctatttc ttctttaatc cactttaaat attctttgct tcctttatga
    19141 attttaaaag aaattatttc cgggatttta taaggatgat tttcttttat aattttttct
    19201 atttttttat acatttcctc ctttgttttt ataaataaaa gaatttcttt tcctttctca
    19261 attttccctt cccaccagta ataactttca atattttcaa ttatctgaat acatactgca
    19321 actctctttt taagcagaat attagcaagt aatttagcat cttttttcct ctcaaaagtt
    19381 gttataattt gtataaaatt attttttccc atttttaact caaatttttt attatttctt
    19441 tataattcct gaaataaatt ttataataac aaaaattata aaaatccata ttaaaacaat
    19501 tatagttcca gcagtccatc ttaatttttt gcttatatcc tcagaaattg ccctttcttt
    19561 acattctttc cagacatatt caggtatcat ttttattgaa aagtacactc ctattgaaag
    19621 gataactata tcatcaagat atccaacaac aggtataaat tcaggaatta aatccaatgg
    19681 acttaaaata tatcctacaa tacaaacaat aaaaagtttt gcatataatg gaactcttgg
    19741 gtctctatat gcaagataaa gagcatgagt atctcttttt aaaatttctg tcttcccttt
    19801 caaaaaagaa gttattttca ttttttattt aattataaaa gttctaaaat tttttttgtt
    19861 cttcaatacc atatttgact ttaatttact caggaattaa atatttatct cttgaaacaa
    19921 tctctgaaaa aagttctttg ccagaagaaa aaacagttct ggtagttgta gtaagtaaaa
    19981 attgttcctg ttttattgtt tttgcaacat atacttttat ctcacggact tttggttttg
    20041 ttcccaatat tgttattttt aaactatttc cttctacttc agattttatc ataagattgc
    20101 aggtataagg gttataaaat ttcaggtcaa ttccttcgtc aattgaaact gttgcatcaa
    20161 gaccgggtgg aatatatgaa actaattttg agtgtggttt tctttcaaat atggaaagac
    20221 cagcataaag aacagcagca taaagagttg atgaaacctg acataatcca cctccaagtc
    20281 ctaaaactct cttgtcacca ataagagtag atgctgttcc aagttcacca tcagaaattt
    20341 tctctgtaat ttcattatta aatgaaaata taaccctggg aaaaagaatt tttccatcaa
    20401 gatattttgc acctgtttct atattatttc ctctttcatc aatgttttgt tgaaaataag
    20461 ttgtgtaaga agaccattta tatttgaact ggtcagccat acaaatagaa ttcaaaaaaa
    20521 ttaaaataaa aactattttt atataatttt ttatcatttt tccttaatta ttaaattatt
    20581 tccctgagaa tgtccccaga tttctggtaa atacatcaat tgtgcttttg ttggaagaat
    20641 attatatgaa ccgggagttt ctgctcttaa ataataaaca attgtttttt cttttgctcc
    20701 ccaggaagtt gtgaaaaaaa caattttttc atctcttatt tctcttctgc aataccacca
    20761 attatcttct gataactcat taacaacttc acatcctgat ggaattgggt cttctatcat
    20821 tacatattca tatttttctc cacccgaaat ttttatttca acacggattt tttcaccact
    20881 tttaacttct ctattgatat catcataaac atagattcta ttcccttctt tatcaattgt
    20941 ttctcttgat tttatttcag tatatcttcg taatatctta aatccgccat caaaaggtgg
    21001 aatagagata tcttttacag aaaatttaat tacattagaa taataaagat taccctgtcc
    21061 ttttttctct atctctattg aatttatttt tcctatgctc agattttctg tatttatatc
    21121 aatagttgtc ataaacttct taatactttc tctatcaatt ttttcttctg caatattttt
    21181 tttattgact gaaagaatga aagtataatc aggtgataac tcatctgtaa tcataagata
    21241 attggtgaaa gcaaaaagac atatagcagt atctttcgtt gacatccaga gtcctccctg
    21301 ctttttcata acaagatacc ttataataaa aggaatttct tctcttttag gatttattgc
    21361 cagtattgca cataatcccc atgcagttgc ttcaatatta ttatgaatcc aggaataatt
    21421 tccgtcttca actgcccagt aattaagata tgtagtaggc tttacaagtt tggtgcatag
    21481 ttcatcaagt aaagtatttg ctcttgtgtc tcctctttcc tttaaaataa ggcataattg
    21541 agcaatggta taggaattta gtttatctct gttattataa atttcttcaa caactggtaa
    21601 gctgttttca ccagtatatg ttagaacata aagtataaat tctttctgat tgtagtcatt
    21661 tacagtatta agcatatttt tcaagcattc ttttcctctt tggtatctat cctcatttac
    21721 aatatatcct gattttttgg catgagttaa tccaaatatc acatatccag ttatatatgg
    21781 attgcttata tcgttttgcc accaccccca tcctccatct tcattctggt agttataaag
    21841 tttaatcaat ccatcactta cttttttagg caattctttt aacatttgtt caaattttat
    21901 tctatcagat gcaagaaaat aaaggtcata tgatttaatt tttgtaagtg tatctgcaac
    21961 ctgaatagca gggagaaaac cattgagtgt ttgttcaata catccatatg gatattttga
    22021 aagtgcttca agagaagtga acataccaga aacaagagag ggataaatat aagaccttat
    22081 gtcaattgaa gaaggaattg ttccaatggg taaataaaat gtatcaactc taacatcatc
    22141 acactgacct gcaaatacat atctttcatc tgtaccatat aaaaaaacag gtattttaag
    22201 ttccatacca tcagaaaatt cagatgattt tgcatagagt gtaattatta cctgtttaac
    22261 tgaatttact ttcactttcc agtcaactct tgcggattta ccagatggta attcaattat
    22321 tttttctgtt tcgtcaagaa tatcaagtcc ttctgcatgt atttttgtat aaattttttg
    22381 ttgacttcca gtataattat gtataactcc tgaaattaaa agtcggtcat tttcaacaaa
    22441 aaatcttggt gttatcaaat ttgccataag tggttttgca gttgtaatat tattttttcc
    22501 tgtaccaact tttgtatcat cagtaacaga acgaattgta gttctccatg ttgtaaggtt
    22561 atctggaagt tcaacttcaa tttttcctat accattttca tctgttaatg caagaggtaa
    22621 ccagaaggca gtatctttaa agttttttct tatatcctgc tgtgcaaagt cctttccagc
    22681 accgccataa taccatctgt aaaaagaata tccagttata acattatttc ctttcaaacc
    22741 ataaaaaaag tcctctattt ctggtacaag ttcgtttgaa attgaataaa tcgcttcatc
    22801 aaccactccc attgaaaatt cagcagaaac aggatttcct gtattatctt ttattttaat
    22861 cagatatttt gctttttctc ctggttggta ttttggttta tcagaagtta tttcaacatt
    22921 aagaaattta tcatcacagg atacaacgag agatttgctt gaatacagaa aacttttgtt
    22981 ccttacagcg cagattgtaa tgtaaatatt tggaatgaat tcatatttaa ttggaagttc
    23041 taacaaaaca gaatttccat tcattttaat tactctattt tcaaaaattt catagccctc
    23101 aattgtataa agaattgtta aatcactata agaggagttt ataagaattt ttgcagtttc
    23161 tcctggctta tattttttct tatcagatac aatttcaagt tcttttttac cataccattg
    23221 agaataataa tctctgccag ttatccaaac atattttgtt gcagagatga tattattata
    23281 ttcatcaatt ccctgaacaa taatttttat gtaccctgtt atacctggtt ttatattgaa
    23341 agttgttttc gcattgttaa gaataacatt ttttgaaata atattattat aactccatct
    23401 tcttttgttc ttatcatact tttcctgact gactattagt tttaattgtt ttttcccttt
    23461 gaattctcgc ccatcataat attttattga aaggtaaaca ggaatttctt cttcaggaga
    23521 ataaatatat tttgatgttg ttattgctat ttcaaaactt ccaggaacaa ttactatttt
    23581 tgtactttgt attatttctc ttctgctttc atctgtcatc ctaacctcaa tcgtaaatat
    23641 ttgttttatt tcatatgaaa gttgtgttgg aatttcaatt aaggaaatac cattttcatc
    23701 tgttgtccct ttcccagaac aaacaagtcc acctttccac catcctccat attcttcacc
    23761 atattcataa tcatcattat cattttccca tatttgtctt gaatatatag cccataacac
    23821 atctgtattt tttacaggtg ttccaaagta atattttgct gtaataagaa ctttaatttt
    23881 ttcacctgga agataaacat ttttatctgt cttgatatca atagaaaatt ctggcttcct
    23941 atattctaaa accttaaaat tacaataacc acttaattca tttttcccaa tagtcataaa
    24001 ttcaattttg tatgttccaa gtggaggttc ttctggcagg gtgtatgaac cataaataga
    24061 attagttgtt cctggaataa tttgtgtttc ataaacaact gtttcatcag gtaattttac
    24121 tttaatctca catgaagaaa attctgctac agaatattta tctcccatat caatccttgg
    24181 aattgcttta aaattaacta cctgccctgg tctataaaca ggtctatcag tataagcata
    24241 gactgttttg ttctcataag tcccgccata tattgaatta caggtgtctg aaaatgcaaa
    24301 agaatttcct ttgacagcaa taaacttatt aaaactttcg aaattacact gaccattact
    24361 atctgttgtt gtagttgctt ttaaattcca taaattatca aataaaaata tttgaatatc
    24421 agggagaata tcaccttttg tgagagagaa taatgtaata ttcgtttttt tattggatat
    24481 ttttgagatg aatccaatgt cagagacaaa aaaccatcct tttatttctg caaatccttc
    24541 tggttttaat gcaaaaacat atgccccttc aggaaattgg tccaataaaa aatttctgtc
    24601 ataaagttca gttaatggag taggatatgt aatttcaaac tttttactta gaacaggact
    24661 cttccctgat aaatcagaag acatcaggat ttcatttatg gaaatgtctc ttaattttgt
    24721 cttatttatt tcattaagca ggtcaatttc atatacatca attgtggcac tttttattct
    24781 aaatgcatta aagtacactt taactttttc ttctggagta aataaccatt cttttgaggc
    24841 aagatgaaaa tattttgctc taattttcat ctgtattttc cctgcatcaa cagtttcttt
    24901 tggttttaaa tcaaacttct tttcaattct tatgtcttca tatccctgtc tatatgcata
    24961 taaactataa tatcctggtt tcaacccatc aatatagaaa taaccgtttt tatttgtttt
    25021 tgttgaacaa aaagaagtac ctgaacaaga aatatccaca ttatctattg gcttttctgt
    25081 ttcagaatca acaataattc cttttatact tcctgcataa ggaagggaaa aatttattcc
    25141 acctgcttct ttcccttctt caacaacagt atttttatgt tctttaacaa aaccgtctgc
    25201 ttgaacatag atatattttg ttccaggatt taatccatca agtttataaa atcccttgct
    25261 atttgtcagt gtataaatat ccttggatat aaataccttt gctcctgaaa ttggttttcc
    25321 ttcctggtca tatacaaacc cgcttattgc tcctggaata ctcattatga tttcgtattt
    25381 tacaactcca aattttgctt gatttacctc aatttttcca agtgtactat cgcaaaatcc
    25441 ttttttttga acaacaattt tgtaatagcc ggtctcaaga ttatcaattt tgaattttcc
    25501 attactatct gaaataactg ttttcaatgg agttttacct gtttgaggaa tattgaagat
    25561 agagacattg gcgctactaa ttgctttttt ttcttttgtc tgtataattc ctgtaattgt
    25621 gtcacaaaag cagggaaagg aaaaaacaaa aataaaaaaa taaaaaaatt ttttcatttt
    25681 tcaccttcta taaaaacttt tgctttgaac ttaaaaattg taattccttg ttcatcatta
    25741 atacctgcat ttcttaaaca catttttagt gcatattcac ctgtttttgc ttcttttgga
    25801 agaacaagac cctgtttgcc agatttttta acaataagtc cttcattttc aggattataa
    25861 aaagagatag aagatatttc aacaggaatt tcaggaattg ttatctgtat ctttatgtct
    25921 tttaattcct gtaaatcaac aggtctatac ctataatcct cagtagcagc aataattgaa
    25981 aattttataa ggtctccagc aagtgttccc gtttctgttt taaaactacc agaacaacct
    26041 ctagtttgat tccctttttt gagagaaaca aaaactggaa ttggtttatt aaaaatttct
    26101 gggagatttt caggaattat ttcgctatac cctgttatat aattttcaat tgtctttcta
    26161 cagatgaata tcgctttttc ttcaaaaact gatggaaaaa aaatatttac tgctaaacaa
    26221 agaactaaaa aattcctgga aatctttgag taataattca gtaattttct ttttatctta
    26281 ttatacatgg gaaaaatatt taaatttttt aaaacaaaga ttatagttaa attttcaaat
    26341 aattttttca aattgtcaaa taaagtgtaa tttgagagaa aggaaaatac tttttatcta
    26401 attttttgtt ttgggaattg tttttcctga taaatagtaa agtcctttct ctctatgata
    26461 tttttcattt cttcacaatt acaattattt tctggtattt ttttgtctgg aatttgtcta
    26521 ttttataagg gctctaattg acttgacaaa ctcaatatat tgtggtacat tataaataaa
    26581 ggtacaatat atagaaaaat gaaatgtcca tattgcagtt tccttaaaga caaagttatt
    26641 gatacaagag aaataaataa cgggttgcag gtaagaagaa gaagagaatg tttaaattgt
    26701 agaaaaagat ttactacata tgaagagata gaaatgaaac caattatagt tattaaaaaa
    26761 gatggaagga gagaaaaatt taacagggat aaaatttttt ctggaataca gagagcatgt
    26821 gaaaaaagac ccatcagtac agaagaaatt gaaaaagtaa ttattgaaat agaacaggaa
    26881 ttgagacaga aaggcgaaag agaaatacca tctaaggaaa taggtaaaat tgttattaag
    26941 aagttaaaaa aattagataa aattgcatat ataaggtttg cttctgttta tagggacttc
    27001 caggatgttt cacaatttaa aaaggaaata gataaaatta tatagaggag ggaaaatgtt
    27061 tgagaaaata aagaaaagaa atggtgaaat tgtaaatttt gatgcttcaa agataacaaa
    27121 tgcaatttta aaggcaggac aggcaacagg tgaatttgga tttgatattg caaaaaaatt
    27181 aaccttaaga gtccttgata tcgcaattaa tacaataggt gaaaaaatac caaatgttga
    27241 agaaatccag gatattgttg aagaagttct aatttcttct ccgtataaaa aaacagcaaa
    27301 agcatacata atttacaggg aacagcatgc caaaataaga gaaattacaa ctaaagcaag
    27361 tttagacctt gttgaccagt atttaactaa aattgactgg cgagtaaatg agaatagtaa
    27421 tatgagtttt tctcttcaag ggctaaataa ttatatttca tctgaaatta caaaaacata
    27481 ctggttaaat aatatttatc ctcctgaaat aagaaatgcc tatataaacg gtgattttca
    27541 tattcatgat ttaggatgtc tttctgttta ttgtgttgga tgggatttac aggatctttt
    27601 gactgttggt tttaagggtg cagcaggaaa agttgaaagt aatccagcaa aacatttcag
    27661 gtcggcatta ggtcagatag ttaatttttt ctatacactt caaggagaag cagcaggcgc
    27721 tcaggcacta tccaattttg atactttgct tgctcctttt gtaaaaaatg atgaacttcg
    27781 ctataaagat gtaaaacagg caattcagga atttttattt aatgtaaatg tcccaacaag
    27841 aactggattt caaacacctt ttgtaaattt gacatttgac ctggtagtcc ctgaaatata
    27901 caaaaatcag ccagtaatta tagggggaga atttaaaaat caaacatatt ctgaatttca
    27961 ggaagaaatg gatatgataa accgtgcttt tcttgaagta atgcttgaag gagatgctaa
    28021 aggaaggcct tttacttttc caataccaac ttataatata acaaaagatt tcgactggga
    28081 taactcaaat attaaactcc tgtgggaaat aacagggaaa tatggtatcc cctatttttc
    28141 taattttata aattctgata tgagcccgga tgatgttcgt tcaatgtgtt gtcggttgag
    28201 attggataaa aaagaattga ggaaaagagg gggaggactt tttggtgcta atcctcaaac
    28261 aggcagtatt ggggttgtaa cgattgattt acctcgtatt ggatatttat ctaaaacaaa
    28321 agaagaattt aagcagcgat tggaaaggtt aatggaactt gcaagagaaa gtttagaaat
    28381 caaaagaaaa gttcttgaga aatttacaga tgaaggcctc tatccatatt caaaatttta
    28441 tttaagaagt gtaaaagaaa gaacaaaaaa atactggacc aatcattttt caacaatagg
    28501 tataattgga atgaatgaag catgtcttaa tctttttggg aaagatatag gaacacaaga
    28561 gggaagagat tttgctattg agattatgaa ttttattaga gaaaaactta ttaaattcca
    28621 ggaagaaaca ggaaatttat ataatttaga agcaactccc gcagaaggga cttcttatcg
    28681 tctggctaaa atagataaaa caaaatatcc agatataata gtggcaaatg aagaaagtta
    28741 taaaaaagga gcagaaccat tttatacaaa ctctacccaa cttccagtaa attatacaga
    28801 tgatattttc ctggcatttg aattacagga tgcacttcaa acattatata caggaggaac
    28861 agtttttcat atttttattg gagaaaaaat acattctgcc aatgggataa aaagtttagt
    28921 aaaaacaata tgtgagaatt ttcatcttcc atattttaca attacaccaa ctttttcaat
    28981 atgtccaaac gatggatata tttctggtga gcattttgaa tgtccaagat gtaagtcaga
    29041 atgtgaggtc tattcaagag tagttggtta tttaaggcct ataagtcagt ggaataaagg
    29101 aaaacaggaa gaatttaaaa taagaaaaac attttctgta aaatgaaaat aggtggatta
    29161 caaaaacttt cgttgattga gtatgatggg attttatcct gtgttgtttt tactattggt
    29221 tgtaatttta gatgtcccta ttgtcataat ccagaattgg ttttacctga aaagtttcca
    29281 gtagaaatcc ctcttgaaga tttctttcag ttccttaaac aaagacaaaa atatctaaaa
    29341 ggggtgtgta taacaggagg agaaccaaca attcagacag accttcctgt ttttataaaa
    29401 gaaattaaag aacttgggtt ttctgtaaaa cttgacacaa atggctcaaa tccagaaatg
    29461 ttagaggacc tgataggaaa gggaaatatt gattatattg cattagatgt aaaaggacca
    29521 ttagaaaaat ataatgaaat tgtaaaagtt gaagttgata cagaaaaaat tttaaaaagt
    29581 atttatctaa taaaaaattc agcaattcct tatgaattta gaacaacagt tgtaaaatca
    29641 caactttctt ttgatgattt taaaaaaatt gggaaaacaa tttcaggatg taagaactat
    29701 tatctccaga aattcattcc ttcaaaatgt gttgaccctt cttttataaa tgaaccaact
    29761 tattctgatg atgaacttga gaaaataaaa gaaataatgc taaattatgt agaaaattgt
    29821 tatataagat aatttcatga ataaattttg attaattttt taaatcttct ttgcaagaat
    29881 attttataag ggtataatgt ctgaaaaaac tatatacctt tttatacaac ggagggagaa
    29941 tggggaaatt agcaataaat gggggaaaga aagttgttga taaag
//
LOCUS       DAOOVW010000020        29328 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_1081_length_29328_cov_8.953097, whole genome shotgun sequence.
ACCESSION   DAOOVW010000020 DAOOVW010000000
VERSION     DAOOVW010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 29328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29328
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1081_length_29328_cov_8.953097"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(317..1786)
                     /locus_tag="PLW95_04100"
     CDS             complement(317..1786)
                     /locus_tag="PLW95_04100"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOV21845.1"
                     /translation="MYLGIDLGTSKIAVVLYNEKENKIIFSSSYAHNALKIKDKFYEI
                     DPEKIKRICFDIFKNIPYKKEIKGIGISTQMHGCLIVDKNFNPLTPLITWEDKRTLLS
                     FKYNKTYIEYIKDICKNIENTGTTISTGFMGATIFYLKENNPDLFRKENKVCFLGDYI
                     ASCLTKEKIKTDITCAGSSGIFDIVKKEWLWNTIEKLKIPKYIFPEVVETGEILGTIN
                     KKVAKELGINEKCRVAVSIGDNQASILGCAGYLKNKLVLNIGTGSQISKNIGNYKEIK
                     YCDTRYFIKNKYIVVGAGLSGGKTLKILEKFLKEVGGKIFNKKEPSLYKKMHKIAEKN
                     KSNNIITGTFFSGSRIFPEIKGFIINLSEENFKLENFIFSFYLGVIFELYFYYKNIGG
                     NKEEKIIGSGNGFRKNPLFAKISSSIFNLPVSIPLTKEEAATGTCLVIAEKLSGIDIE
                     EFIRDNTNRDVYYPDKNLKYLQQLYDTYEDTIKNLTKIF"
     gene            complement(1787..2560)
                     /locus_tag="PLW95_04105"
     CDS             complement(1787..2560)
                     /locus_tag="PLW95_04105"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOV21846.1"
                     /translation="MTLYNKSEIVVEYNLYDNLPYLEINGKVNWQEKKKFLKISLPVN
                     LKNQRIFCEIPMSFVERKDDGEEHPFQRWIKISGDFKNEKYGICVINDGFYGYDFDGK
                     ELRLSILRSPRFAYHHSLNTLGKEKILKESDYIDNGINRFKLWLIPYKNDNINPSKYA
                     TFLNKEIEYILPLSHKGILPSENSFVKVEKENIIVEVLKKGEDGNFLILRAYETSGKL
                     TETNLEIFGKKYRVNFGKFEIKTLKIKDNKITEVNLLEK"
     gene            complement(2824..4308)
                     /locus_tag="PLW95_04110"
     CDS             complement(2824..4308)
                     /locus_tag="PLW95_04110"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOV21847.1"
                     /translation="MTKDYFHNIYGCWLGKNIGGSIGCPIEGKKDFMSLPYKYPEEIF
                     ANDDIDLQLVWLDILKKKGIKITTDDLADGWIRNIDYPFDEYGVAKANLSLGLKPPIT
                     GIYNNWFCNCMGASIRSEIWACINPGRPEVAGWYAYQDASVDHWNEGVYGEVFLSVLE
                     SITFVEKDIEKAINKAIEFLPETSNVYNVVKLTQLLEKEGRSLKEIREKIIEKFGHHN
                     FTDCVQNIGFIVLGLLKGQGNFLKTIIESVNCGYDTDCTGATAGAIIGIIVGKEEIIK
                     QVKTKIDERIIAGWGIKGIKVPENINELSNEIVEIEEKAIQEKGLPMIEKPFKLPEIE
                     SYAKPLKIPFQISEEFSLNGIDEIEKKILDGKYKNYKEVIFDEFYFPLKNYFKNNGQV
                     CIFLKTKIKNKEKRKIKLFPSSNDGIKMWVDKKLVLSHHYHNDFLPAPHRPGSPLIEF
                     ELDKGVYEILIEVLKCRDDLEFAWIIGDGKNHLVVDMEYERHIF"
     gene            complement(4527..6092)
                     /gene="cimA"
                     /locus_tag="PLW95_04115"
     CDS             complement(4527..6092)
                     /gene="cimA"
                     /locus_tag="PLW95_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HOV21848.1"
                     /translation="MGKIEIYDTTLRDGSQAEGVSFTLEDKIKVAEILDDFGIDFIEG
                     GWPGSNPKDISFFEKIRRKNFKKSKIVAFGSTRRKNCQAEKDPFLKKLLESETEYITI
                     FGKSWDLHVTDILRISLDENLRMIEDTIKYFSSMGRKVFFDAEHFFDGYKRNSEYALK
                     TIKVAEQFGAERIILCDTNGGTLPFEIEKIIKDIKGKIKIPLGIHTHNDSGVAVANSI
                     VAVKEGCIQVQGTMNGLGERCGNTDLTSIIPILQFKMGYKCIEENKLPFLTEISRKIY
                     ELANMVPVNNQPFVGLSSFSHKGGVHIDAVSKRSEAYEHIDPSLIGNKRKFLISELSG
                     KSTILQKLKGYDIEKRPELVKKILDMVGELENTGYQFESAEGSFELMVSKSLGKFEKF
                     FQVEGFRVIVEKRGKRVISEATVKIKLDRSIELTASEGNGPVNALDNALRKAIEKIYP
                     EIKNLKLTDYRVRILHPERATGALTRVIIQSVDEKDSWGTIGVSTDIIEASWNALLDS
                     FEYKLYKDKKQQI"
     gene            complement(6147..6791)
                     /locus_tag="PLW95_04120"
     CDS             complement(6147..6791)
                     /locus_tag="PLW95_04120"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOV21849.1"
                     /translation="MIKKQQISSETIKRLAVYLRYLEKLYREGVKIISSSHISKILNI
                     PSSQFRKDLSFFGEFGKRGVGYDIGKLIREIKKIIGIDEKIRVIIIGVGRLGSALIGY
                     PGFSDLNIEISGAFDKSPEKVGKKIENIIIRNISGIGEFIKKEKIKVAVICVPANQAQ
                     NVCDILVENSIKGILNFAPVKLLANENISISNVDVASEICNLIYLLKNSYAKSQ"
     gene            complement(6792..7598)
                     /locus_tag="PLW95_04125"
     CDS             complement(6792..7598)
                     /locus_tag="PLW95_04125"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003248446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase subunit"
                     /protein_id="HOV21850.1"
                     /translation="MVSISLKEEVVIVTGGCLGIGKSISLKLREAEAFVVIADINEKI
                     GKEMEKEFGGENIYFVKVDVTKKDEIEEMVKIVKEKYGKIDALVNNAGINTPRLLVDP
                     DGKEEVTEDIFDKMVAVNQKGAFFCAQAVAREMIKNKKGVILNVSSESGLEGSEGQSV
                     YAGTKASLYSYTRSWAKELGKFNIRVVGIAPGILEPTSLRTPEYERSLAYTRGITVEQ
                     LRKSYENVSIPLGRVGKLEEVANVVLFLLSDMASYITGTVINISGGKSRA"
     gene            7684..10089
                     /locus_tag="PLW95_04130"
     CDS             7684..10089
                     /locus_tag="PLW95_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV21851.1"
                     /translation="MEKYKVPIEKLRKECPSDYFKFESTQQVNPPEDIIGQERAKRAL
                     EFGLKIKSTGYNIYVSGIPGTGKTTSVEQIIKLLAENQPVPDDWVYVYNFTSPDNPKS
                     LRFPPGKANIFKKDIEGLINDLKLEFSRVFEGEVYVAHKNQILKDFQNKSDKLFDQLE
                     ESAKKAGFQIKRTPSGILFIPTIEGKPLNEAEIEQLTEQFKEEIKKKQEILYEQLDEI
                     LRQIRQFEKETKTRLSQLEKETAKYILSPKIGELKEKYSIQKYVIDYLDELEKDIVEN
                     VDEFKEKKEIEILPGMKLPERESTLFKYSVNVLIDNSKTKGAPIIEETNPTCYNLCGR
                     IEYRPQFGAMITDFTMIKPGALHKANGGYLILQVFDVLKNYFSWEILKRALKNKQIII
                     EDLNEQFRLINTPTLRPEPIPFDTKVILIGNPLYYYLLYYYDEDMKKLFKVKADFSTI
                     MDRNKEGIESYTSFISKICNEENLKPFDKEAVAKVIEYGSRLVEDQEKLTTRFIEIAD
                     IIREANFWAEEENSNIVKKEHVKKALEEKIYRSNLIEKRIEELIKEGTIMVDVDGEKI
                     GQINGLSVMALGDYSFGKPSRITATISVGRDGVINIDREVKLAGTIHNKGFLIINNYI
                     KEKFGQKKAITFSGSICFEQLYEEIEGDSASSTELYVLLSVLSGLPIKQGIAVTGSVN
                     QKGEIQPIGGVNEKIEGFYYTCKSIGLTGNQGVIIPEKNVKNLMLNDEVIEAVKNGKF
                     HIWSVKTIDEGIEILTRVPAGNMKEDGTYPENTVNYIVGKKIDELTEKYIETQKPGKS
                     KKK"
     gene            complement(10101..10991)
                     /gene="era"
                     /locus_tag="PLW95_04135"
     CDS             complement(10101..10991)
                     /gene="era"
                     /locus_tag="PLW95_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOV21852.1"
                     /translation="MKSGFVTIVGRVNVGKSTLLNNILNKKVSIVSSKPATTRIKILG
                     IYNCEEGQIIFIDTPGIEKWRNQLGKFMQKMILSSIDETDIILFLIDAKGWREEDEKI
                     LEVLKKTNKKILLVINKIDLLKDKEELLPLIDELNKKYEFLDIVPISALKKQNVDRLI
                     KVIISHLPEGEKFYPSDVDTNLPTEYKIGEIIREKILEKTHQEVPQEVAVEVEEIREG
                     QKNKDVIFISANIIVERDNLKGIIIGKGGERIRKIGQLAREEIEFLLGKNVYLQLKVK
                     VIKDWRNRPDIFRRFGYGIF"
     gene            complement(10988..12091)
                     /locus_tag="PLW95_04140"
     CDS             complement(10988..12091)
                     /locus_tag="PLW95_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013782479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOV21853.1"
                     /translation="MTNRERWRRIFEFEEVDRIFNCEFGWWNETFLKWHKEGLPEYIN
                     NNNKGDIYFKFDRQEIIPINLGLLPYFEEEIIEETGRHIIKKDSSGRILQIFKDGSSS
                     IPHFLKFPVENRNDWNEFKERLQPDIEKRYPSSWESLKKEWGKRDYPLGINVGSLFGW
                     VRDWMGFENCLISFYTQPDLIEDIMGYVANFVLTLNEKALEEVKGIDFACFWEDMAFK
                     TQPMISPQLFEKFMVPKYKKITSKLKKEEIDVVIVDCDGNINQLVPLWLSAGVNVMFP
                     LEINSGSDPVVLRKKYGKNVLLKGGINKIALINGEKSIDNELERIKEFVNSGGYIPHV
                     DHRVPPDVSYENYIYYLKRKKEIFKIDDWEIPE"
     gene            complement(12096..13109)
                     /locus_tag="PLW95_04145"
     CDS             complement(12096..13109)
                     /locus_tag="PLW95_04145"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOV21854.1"
                     /translation="MANVGILGAGFMGEMHSTVYSQLPDVKIVGIADIRGEKAKSLAR
                     KFKTIPYFDPEQILKKPDIQIVDVCLPTFLHKEYVIKAAENKKDVICEKPIALTVEDA
                     NEMIKVCKENNVKFMVAQVIRFWPEYKFLKDAYDSKRYGDLKLISMRRLSPLPTWGWE
                     NWLLSQEKSGGALVDLHIHDTDFLLYLLGEKPKKIFSKIVKNYNNDANVLTTFTFGNG
                     IVAHADGEWDFPPSFPFEMSYIAKFEKGTIEFNSRNTPTVVAYGANGNVERPTFEKIK
                     VEGIEGNIEDMGGYFYELRYFIDHVIHNKPFQVVTPEDARDSLYVVLKEKESAEKGQE
                     IIL"
     gene            complement(13151..14983)
                     /locus_tag="PLW95_04150"
     CDS             complement(13151..14983)
                     /locus_tag="PLW95_04150"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOV21855.1"
                     /translation="MKKFLFLFFLFSFICFSSEWYEKNKNILVVFPDYIKNYLKKADE
                     FDTKGMTSYADYYLRLTETKIQSVLPFSPSNWPNGWPQDREAMKYLKYATPDAYLYRI
                     IGDYALSHNKNKEGIKYLQMYLSKCIIPDTTYMMKLATIYQKENMLRDAEGIYEEIRN
                     VVETKNFFGEKYSVSYLTSKIKNIQLLLKNTNILTLNVSYNNVPDFLKSDFQDLFLGI
                     FKEEVKNINLITNEQLKKVLEEENITEEDIGDEEDLSYVAKILNADYVIKPLLSRIES
                     QYIFEINVFDPKKKIFFENYEFKADDIRFLPNIIERFCYKFQEKDIPGFLYIPENQLI
                     WSFEADSLISDIKISDDGKRTILGTEGGYVYILSDKGNIIRKLSFPEKIVKVAVSPDG
                     QYFSFYCLGGNIYFLNKYGTVLWNKKLNNYGRGIDISKDGRFLVIGVNNNVYYVDNNG
                     EIFWDLEVSKNIDIIKISSDAHWVFAGLENGVVCCFSDDGNERWRKNVEGKIEDIKIT
                     TDTNFICVSTKKGQIYLFNGDGTLIKNFSSDEDIDFAIFSPSVLDILGGKKGNYFYFL
                     SYDKKGLWKYQLQEKIDFAVGLVDGKLVQTVEKNNVFSFSIKWQ"
     gene            complement(14980..17595)
                     /locus_tag="PLW95_04155"
     CDS             complement(14980..17595)
                     /locus_tag="PLW95_04155"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOV21856.1"
                     /translation="MKTNKLFIGFLFIFFSFYLFSAELDTNVVKYGTSSDKINFINKV
                     LSKGDKTYLPDIAKLLDDKDEIVRSKVAYTLWKIGDSTCVDYYKKGLEDSYWQVRYYS
                     IRGLVEFASGEEILQFHKALDDSYWQVRYYAASGIGKYGNSDSIDVLVRKINDNKEKV
                     KEEILWSLTRLMWKAENREKFKNLPDSDVQNIYNLLKSNNVFLKIRTIYLIESTEDSR
                     GISYLTNALDDENDEVKIRSVWALEKLKATDAADKIESLLAAPSVKVKIESIKTLVRL
                     KTEEGLEGILKGLNDPDESVRIYSLWALEKFRNVSTYPEIVNALGDNSQSVMEYAIKI
                     IENIKDTAFIPALEKFVDDENNSINSRLSALNLIGKVGDESVKNYLIEKTKDPNQLIR
                     YGAIVAFSLLDMFDVQYLEILSYHEKNETCGRVKEESRKILRDVVNKLNEKIKSSNPS
                     DRKFVIDIVDAIIQTDEVKHLIYKMLLSNYSDVKNKAIEVVLRKPERFFAKGIKDALL
                     DSDIETRKISTLAIGEIGDKTAIPILQKNLKHNDPELQLYSAYSLAKMGQKDVFPYAM
                     KFINSSNPQYRKKAAEIFGYIKDKRSIGLLLQALEDEELDVKLAASYSLSKMGEIKGI
                     EFLVRISEENIEPLRSQANRYLNDSSIPLSLRGKIPQIRQALYKEKLGIQEVTEKIIY
                     AHRIEGTINIDGKDDDKYWDMVKMSNEFILIEKEKVPYNIQTKVAVGYDEKNLYFLII
                     CEDQGSREITLNTRDFITISIAPYNSKGQWYQFVLHPMGLIRYSYIWKFYTDNDKEKN
                     WQSNWIAKSNVESNRWIAEISIPLSEFEINEIKNGDKWRINFLREIENRSTSTWTGRI
                     DIPEQFGIMLFKESL"
     gene            complement(17582..18247)
                     /locus_tag="PLW95_04160"
     CDS             complement(17582..18247)
                     /locus_tag="PLW95_04160"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HOV21857.1"
                     /translation="MKKIVLSSEEIKKIEDETEKKYGIIKLILMENAGRCVSEETKKI
                     IKNKKRKIEIFCGKGNNGGDGFVTARYLFNGGFDVDVFYIPEENKFSEITKRNFVILK
                     NLGVKIFSVENKFKSVLKYKKCDLIIDAILGTGISGMVNDQVREIVEYINEKVAYKIC
                     VDVPSGLNADTGDVCGVAVKGNLTVSLGFGKKGFYMNEGPAYCGKIKIVDIGFPKFYY
                     ENK"
     gene            complement(18249..19640)
                     /gene="murC"
                     /locus_tag="PLW95_04165"
     CDS             complement(18249..19640)
                     /gene="murC"
                     /locus_tag="PLW95_04165"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOV21858.1"
                     /translation="MRKNDIFERVKRVHIIGIGGIGVSGLAYILLKEGKIISGSDTNL
                     TPITKALEKKGVKIFSPQREENITKDIELIIYSQAIFPDNPEYKKGKQLKIPMMSYPE
                     GVGAIMKNKRGITISGTHGKTTTSALVVHLLKKNKLSPSFLVGGKIIGLGNSGVGKSD
                     FLVVEGCEYKRSFLNYSPEIAVLTNIEKDHLDYYYDIREIKSAFWEFCNNVKKDGFIV
                     YCNEDKNVVEVVKKVKREKISYGFENGDFTGRNIVIKKGRTEFDCYFKGKKISHLKLK
                     IWGKHNVLNSLACIAVGNIIGLPFGDIKKAVETFDGVHRRCEIIGNEKGILIIDDYGH
                     HPTEIKATLSTLKTVFPGRRLIVVFQPHQYSRTRFLLKDFARSFSNADKIVVPDIYFV
                     RDSLKEKKLVNAEILVEKIRKNGKEALYLPTFEEIVEYLLEVLKVNDIVLTLGAGPVN
                     LVGRELLEKLRGN"
     gene            complement(19627..20706)
                     /locus_tag="PLW95_04170"
     CDS             complement(19627..20706)
                     /locus_tag="PLW95_04170"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOV21859.1"
                     /translation="MHNKEKKIAIVSGPTGGHFFPALSIGDSLIEKGIKVKFFVPERG
                     FIINWLNKRNYNFSIIPEVKISKKGIFIIPFKFLFNIFKCFIFIQKENFVAIVGTGSY
                     TTFPFLVAGKILRKDIYLHEQNYIPGKVTKFFSILSRRTFLTFPYRNKLPEKKVVVTG
                     FPLLKEFKIHISKEEIIENFFLDKNKKVIVVFGGSQSASFLNKLITSNIDYFVKKNDF
                     HIIIIAGKEKVKVEEIFFKKKVSGIVFDFYYNMNELYSIADIVISRAGAGTISELTYW
                     KIPSILIPYPFAGGHQFFNAMFLQEKDACFLIPQREEIVYNFPLYFEKFLGNYEKIKK
                     ALEKVKIYDDGKIVNIITGALNEKK"
     gene            20874..21599
                     /locus_tag="PLW95_04175"
     CDS             20874..21599
                     /locus_tag="PLW95_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21860.1"
                     /translation="MLKKLANEEINWLLLSEKENYEKNIKIIAQKNKIGEGEITEELK
                     TTTELLDIGERIENKIIITPELFKNVFLLALTDFESKLVKYWDTPGEFTKIMIGKTLS
                     DKSMLHLMADILKLKYYREYWSLDAVFYEKEDKEHFQLPFLVAEYLPVVIEHENAISG
                     SCLEMNKLSIFNSPLKVLITYPETEDKNKYLNWYTEILKKADIFDDFSTKRKHLLIFG
                     YKKEEKTFWEFYTYQNKTFSLLE"
     gene            complement(22407..23012)
                     /locus_tag="PLW95_04180"
     CDS             complement(22407..23012)
                     /locus_tag="PLW95_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOV21861.1"
                     /translation="MKIKKIVVGEFFTNCYIIWCEETGKGAIIDPGAESEKIKYQIEK
                     EKIKVEIIINTHGHFDHIGENEKFSLPVYIHKLDEEYLKNPEKNLSILFGIPFICNCQ
                     TKILQEGDIIKIGNISLNVLHTPGHTPGSISLLGENFAFTGDTIFAGGIGRTDFVGGD
                     EEELLNSIKKLLKLPEKTIIYPGHGEISTFSKEISFLTSLF"
     gene            complement(23012..24016)
                     /gene="hypE"
                     /locus_tag="PLW95_04185"
     CDS             complement(23012..24016)
                     /gene="hypE"
                     /locus_tag="PLW95_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008288773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HOV21862.1"
                     /translation="MQKITLSYGDGGKLTYNLIENIFVKHFKNEYLEKLQDASLLKIG
                     KEDICFTTDSYTVSPIFFPGGDIGKLSICGTINDLSVSGATPLFLSSSFIIEEGFDFE
                     TLEKIVISMANESKKENVKITTGDTKVVEKGKVDKIFINTGGIGIKDKRMKLGIERIK
                     LGDLVIINGDIGLHGLSIITKREGIEFESSIKSDCSSLYPIVKEIFPFGEKIKFMRDP
                     TRGGVAATLNEIVIKSDIGIKIYKKKIPISEEVNSLCEILGFDPLYIANEGKIIIIVD
                     RKYGEKVVETMRKQQLGKKAEIIGEVTDKIKRKVLIETEIGVERVVEFQAGAQFPRIC
                     "
     gene            complement(24412..25521)
                     /gene="hypD"
                     /locus_tag="PLW95_04190"
     CDS             complement(24412..25521)
                     /gene="hypD"
                     /locus_tag="PLW95_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015394134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HOV21863.1"
                     /translation="MKYIQEYRNPEIIKKILKLIEKKTEKYDKNINLMEVCGTHTMVI
                     GKFGIRGVLPENINLLSGPGCPVCVSPNSYIDKAIEFSKIENTIITTFGDMMRVPGSY
                     SSLEKEKSKGREIKVVYSPFDALEIAEKRTDKNIIFLGIGFETTSPIVSSTIKIAKRK
                     KIKNFYVFSGHKLIPPAMELLLKDDEVKIDGFICPGHVSTIIGKKPYNFISKNYNIPC
                     VITGFEPLDIVESIYILIEKIMSKQKGEVIIQYKRAVKEDGNRIARKNMEDVFEEIDS
                     EWRGLGNIEKSGLKIKNDFDEFDAEKKFEVKIPEIKKESSCICGEILKGTKKPFDCKL
                     FAKFCNPENPVGPCMVSSEGTCSAYYKYEIPLQST"
     gene            complement(25518..25733)
                     /locus_tag="PLW95_04195"
     CDS             complement(25518..25733)
                     /locus_tag="PLW95_04195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HOV21864.1"
                     /translation="MCLAVPMKVEKVMKDNFAIVSLGTVKKKVNISLVKSVKKGNYLI
                     VHAGFAITKLDKKQAGKTLKLLSEMGK"
     gene            complement(25724..28054)
                     /gene="hypF"
                     /locus_tag="PLW95_04200"
     CDS             complement(25724..28054)
                     /gene="hypF"
                     /locus_tag="PLW95_04200"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HOV21865.1"
                     /translation="MKLLKNKNERVCLKISGIVQGVGFRPTIYRYAKEENLSGFVRNT
                     SEGVYIEIEGPQKNIKNFLKKIKNYPPPRAKIKKIKVIPLSIENEKNFRIIESKKQKS
                     LKVEISPDIATCPDCLKELFDPSNRRYLFPFINCTNCGPRFTIIKNIPYDRKNTTMSE
                     FIMCEKCQQEYNNPESRRFHTQPNCCFDCGPGMFLKNKKGEIVEEGIKSIENTSWFIE
                     KGKIVAIKGIGGYHLACNALNEEVVETLRERKNRIDKPFAIMARDIKTIEEFCYISKE
                     EKGYLNSWQSPIVLLKKKNNKIPSIVAPSNNYLGFMLPYTPIHHILFFLNKNLKVLVM
                     TSGNLSEEPIVYDDKQAFEKLKGIFDFFLTHNREIYINCDDSLLKVFKGSSYFIRRSR
                     GYVPEMIEFPYKFKKNIFSAGSDMSNTFALTKENKVYPSQHIGDIGNVSSIDSYKRSI
                     KLLEKTLEIKPEVICYDIHPQYFSSKIAVSMIEENKDIIGIGVQHHHAHIASVMAENK
                     LKNEKIIGIAFDGTGFGTDKNIWGGEFLICDYTEFERVSHLDYIPLPGGEKAIKEIWR
                     LGCIYLYKTFGNKFLSLDIDFVKNIDIKKWKIIEKMIEKRINCPLTSSMGRLFDAVSS
                     ICSIRNTINYQGQAAIELEMKIEKTKSEPYNYQIKKENNVYIINVEKMIEEIVHDLID
                     KKTVGFISYRFHITISDIIRKVVNIIRQERKINKVALSGGVFQNNFLLEKTYNDLKKD
                     GFELFIHRKVPTNDGGICLGQAVIGNFLLKGEKQCV"
     gene            complement(28035..28478)
                     /locus_tag="PLW95_04205"
     CDS             complement(28035..28478)
                     /locus_tag="PLW95_04205"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOV21866.1"
                     /translation="MKKILIVYCSLGGNTEAAAFAIKKGIKSNEDVEVVIKKGFQADI
                     DDLLSADGIIFGSGDYFGYMGGVLKDFFDRTFYPSRGKVEGKPYFAFLTHGGGGKGIE
                     SIENIAQTFNFKKVMDSIVIKGKPDKNMEEKLFDAGRKFCEIIKK"
     gene            complement(28499..>29328)
                     /locus_tag="PLW95_04210"
     CDS             complement(28499..>29328)
                     /locus_tag="PLW95_04210"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOV21867.1"
                     /translation="EKMIDIHMHIGRLYFEPPLEPEYLLDFMDKNNIEKAVLLPIENP
                     EDTSYYVTTEYVLEICKKYPDRFIPFCNVDPRRREVPNVIKEYKERGCIGFGEVLCGL
                     YVDDVRMQKIYEVCGELKMPIIFDLSGTTCLDEVGLPKFEQMIKKFPETLFIGHGPHF
                     WAEISSDIKKEEFSSYPKGEIKNEGAVIKLLSNYSNSFADLSAGSGYNAITRDVNFGY
                     KFLEKFQNKLFFGTDICHVNQDVPIIQYIKKSREDKKITQSVYEKITEKNIKKIINL"
BASE COUNT         9689 a         5205 c         3319 g        11115 t
ORIGIN      
        1 tgttctgtta tttttacttg aagtgtagaa tatattttat ttaaattctt gacaagtcaa
       61 gttcgccatt tcatttttgg caattatgaa aacacttgta aataaagggg ttaaagagat
      121 tttaatttgt tcactacatt tcactacata tttgcagaaa cccgtatgaa ataaggattt
      181 ttcatttttg agtggcgaag ttgacttgac gacatagaaa aaataaatat aacaaaattg
      241 ccaaaatact tgattttatt agggttttat ggttctataa tatcaagaat cgtcgatctt
      301 gggtaatagg caataattaa aaaatttttg ttaggttctt tattgtgtct tcgtaggtat
      361 catacagttg ttgtaaatat tttaagtttt tatctggata ataaacatct ctatttgtat
      421 tgtctcttat aaattcttct atatcaatac cactaagttt ttcagcaata actaaacatg
      481 ttcctgttgc tgcttcttct tttgtaagtg gaatcgaaac gggaagatta aaaattgaag
      541 aagatatttt agcaaatagg ggatttttcc tgaacccatt tccactgcct ataattttct
      601 cttccttatt cccacctata ttcttgtaat aaaaataaag ttcaaatata actcctaaat
      661 aaaaagaaaa aataaagttc tctaatttaa agttctcttc acttaaattt attatgaaac
      721 cttttatttc aggaaaaatc cttgaaccag aaaaaaatgt ccccgttatt atattattgc
      781 ttttattttt ttctgcaatt ttatgcattt ttttataaag agagggctcc tttttattaa
      841 aaatttttcc tccaacttcc tttaaaaatt tttctaaaat ttttaatgtc tttcctcctg
      901 atagtcctgc tcctactaca atatacttat tttttataaa atatcttgtg tcacaatatt
      961 ttatctcttt ataatttcca atattttttg atatttgact tccagttcct atgtttaaaa
     1021 caagtttatt tttcaggtat ccggcacatc ctaatataga tgcctgatta tctcctatac
     1081 taactgctac cctacatttt tcgtttatac ctaattcttt tgcaaccttt ttgtttattg
     1141 ttcctaatat ctctcctgtt tctacaactt ctggaaatat atattttggt atttttaact
     1201 tctcaattgt attccatagc cactctttct ttactatgtc aaaaattcca cttgaaccag
     1261 cacaggttat atctgttttt atcttttctt ttgtaaggca agacgcaata taatcgccaa
     1321 gaaaacatac tttattttct tttctgaata aatctgggtt attttcttta agataaaaaa
     1381 tagtcgctcc cataaatcca gtagaaattg ttgtcccagt attttctata tttttacaga
     1441 tatcttttat gtattcaatg taagttttat tatatttgaa agataaaagg gttcttttat
     1501 cctcccatgt tattaaggga gtaagggggt tgaaattttt atctactatt agacaaccat
     1561 gcatttgagt tgaaattcca attcctttta tttccttttt gtaaggtatg tttttaaaaa
     1621 tatcaaaaca tattctttta attttttctg gatctatttc atagaatttg tcttttattt
     1681 tcagtgcatt atgagcataa gaagaagaaa aaattatttt attttctttt tcattatata
     1741 aaaccactgc tattttactt gttcctaaat caattccaag atacatttat ttttcaagaa
     1801 ggttgacttc ggttattttg ttatctttta tttttaatgt tttgatttca aatttaccaa
     1861 aatttaccct gtacttcttg ccaaaaattt ccaaatttgt ttctgttagt ttcccagatg
     1921 tttcataagc ccttaatatc aaaaaattcc catcttctcc tttttttaaa acttctacaa
     1981 ttatattttc tttttcaact ttaacaaagg aattttcaga aggtaaaatt cctttgtgag
     2041 atagaggtaa aatatactct atttctttat ttagaaatgt tgcatatttt gaaggattta
     2101 tattatcgtt tttgtaagga attaaccata atttaaatct atttattcca ttatctatgt
     2161 aatcactttc ttttaaaatt ttttcttttc ctaaggtatt taaggaatga tggtaagcaa
     2221 atcttggact ccttaaaatg cttaatctta attcttttcc atcaaagtca tatccataaa
     2281 aaccatcatt tattacacat attccatatt tctcattttt aaagtccccg gatattttta
     2341 tccatcgttg aaatggatgt tcttcgccat catcttttct ttccacaaaa gacatgggta
     2401 tttcacaaaa aattctctga tttttaaggt taactggtaa agatattttt aaaaacttct
     2461 ttttttcctg ccaattaact ttcccattta tttcaagata tggcaaatta tcatataaat
     2521 tgtattcaac aactatctct gatttattat aaagagttat attcgatttt agccaccaaa
     2581 tttaattatc aaaaatattt taaaacggac ttttaaaaat gtccgatttt tcttttgttc
     2641 ttcctaaatc atatcaaaac ccatcttttt attcaatatc tattccgccg cttttatcac
     2701 ttccctttct ttttgttttt ttaatcctcc aaaaaacttt agcggacatc aaaaggtacg
     2761 ttttacactt tgcacttttc ttaaatcttc ccttacccac tcccgccaca aaacatctgg
     2821 acgctaaaat atgtgtcttt catattccat atcaacaact aaatgatttt ttccatcacc
     2881 tattatccac gcaaactcta aatcatctct gcattttaaa acttctatca aaatttcata
     2941 caccccttta tctaattcaa attcaataag tgggctgccc ggtctatgcg gcgctggtaa
     3001 aaaatcatta tgataatgat gagaaagaac aagtttttta tcaacccaca tttttattcc
     3061 atcattactt gatggaaata atttaatttt tcttttttct ttatttttta tttttgtctt
     3121 caaaaatata cacacctgtc cattattttt gaaataattt tttaatggga aataaaattc
     3181 atcaaaaata acttctttat aatttttata tttcccatcc aatatttttt tttcaatttc
     3241 atcaattcca tttaaggaaa attcttctga aatttgaaat ggaattttta gtggttttgc
     3301 ataactctct atttcaggta atttaaatgg tttttctatc attggtagtc ccttttcctg
     3361 tattgccttt tcctcaattt ctactatctc gtttgataat tcatttatat tttctggtac
     3421 tttaattccc tttattcccc atccagcaat tattcgctcg tcaatttttg tttttacctg
     3481 ttttattatc tcttctttcc caactattat ccctattatt gctcctgccg ttgctcctgt
     3541 acaatcagta tcatatccac aatttacact ctctattatt gtctttaaaa aattcccttg
     3601 tccctttaat agtccgagaa ctataaatcc aatattctga acacaatcag taaaattatg
     3661 atgtccaaat ttctcaatta ttttttctct tatttctttc aaacttcttc cttctttttc
     3721 aagtaattgc gtaagtttaa caacattata gacatttgaa gtttctggaa gaaactctat
     3781 tgctttattt attgcttttt caatatcttt ttcaacaaaa gtaattgatt ctaaaacaga
     3841 taaaaatacc tcaccataaa caccttcatt ccagtggtca acagaagcat cctgataggc
     3901 ataccagcca gcaacttctg gtcttcccgg atttatacat gcccatattt cactccttat
     3961 tgatgctccc atacaattac aaaaccagtt attataaatc cctgttattg gtggtttaag
     4021 tcctagtgat aaattcgcct ttgctacccc atattcatca aaaggataat ctatgtttct
     4081 aatccatcca tctgctaaat catctgttgt tatttttatt cctttcttct ttaagatatc
     4141 taaccaaact aattgtaaat ctatatcgtc atttgcaaat atttcttctg gatatttata
     4201 aggcaaactc ataaagtcct tcttcccttc aataggacat cctatacttc cacctatatt
     4261 tttccctaac cagcatccat agatattatg aaaataatct tttgtcatgc actctcctta
     4321 ttattatctc tggttcaatt aaaatctatt ctgctttttt aattttccct tcaatccatc
     4381 taattaatct ttcccaccca atagaggcaa ttttatcaaa cggctgatgt actgttgtta
     4441 gttgggattt tataattgcg gaaagaaaaa tatcatcaaa attagcaaca gatatatctt
     4501 ttttttattt aaatattttt ttatttttat atttgctgtt ttttgtcttt gtatagttta
     4561 tactcaaaac tatcaagaag agcattccat gatgcttcaa ttatatctgt ggaaacacct
     4621 attgtccccc aactatcctt ttcatcaact gattgtatta taactctcgt taaagcacct
     4681 gttgctcttt caggatgtaa aattctaact ctataatcag tcaattttaa attttttatt
     4741 tcaggatata ttttttctat tgcctttctt aaagcattat ctagggcatt tacaggacca
     4801 tttccttcac ttgcagttaa ttcaatactt ctatctaatt ttatttttac ggttgcttct
     4861 gatattaccc ttttccctct tttctcaaca attaccctaa aaccctctac ctgaaaaaac
     4921 ttctcaaatt ttcctaacga tttacttacc attaattcaa aactaccttc cgcactttca
     4981 aattgatatc cagtattttc aagttctcca accatatcaa gtattttctt taccaattca
     5041 ggtctctttt caatatcata accctttaat ttttgtaaaa tagtactttt ccctgaaagt
     5101 tcagaaataa gaaatttcct tttatttcca attaaagaag ggtctatatg ttcatatgct
     5161 tctgaccttt tactaactgc atctatatga actccacctt tatgagaaaa agatgaaagt
     5221 ccaacaaatg gttgattatt tactggcacc atatttgcaa gttcatatat tttccttgat
     5281 atttctgtca aaaatggcaa tttattttct tcaatacact tatagcccat tttgaattga
     5341 agaattggaa taattgaagt aaggtctgta ttcccacacc tttctccaag accgttcatt
     5401 gtcccctgaa cctgaataca tccttctttt actgcaacaa tagaatttgc aacagcaact
     5461 cctgaatcat tatgagtatg aataccaagt ggaattttaa ttttcccttt tatatctttt
     5521 attatttttt caatttcaaa tggaagagtt ccaccatttg tatcacataa aataattctt
     5581 tctgcaccaa attgttctgc caccttaatt gtttttaaag catattcaga gtttctttta
     5641 tacccatcaa aaaagtgttc agcatcaaaa aaaacttttc ttcccattga actaaaatac
     5701 tttattgtat cttctatcat ccttaaattt tcgtccaatg aaatccttaa aatatcagtt
     5761 acatgtaaat cccatgattt accgaaaatt gttatgtatt ctgtttcact ctcaagtaat
     5821 tttttcaaaa atgggtcttt ttctgcctga caattttttc ttcttgtact tccaaatgca
     5881 actattttac tttttttaaa attttttctc cttatttttt caaaaaaact tatatctttt
     5941 ggatttgaac caggccagcc accttcaata aagtcaatac caaaatcatc aagaatctct
     6001 gcaactttaa ttttatcttc aagagtaaat gaaacccctt ctgcctgtga accatctctc
     6061 aaagtagtat cataaatttc tattttcccc atttttttac cttccttaaa ttctataact
     6121 acattcattt cctgtcaatt catttttcac tgtgatttcg cataagaatt tttaagtaaa
     6181 taaataagat tacatatttc agaagccaca tcaacattac ttatagagat attctcattc
     6241 gctaaaagtt ttaccggagc aaaatttagt attcccttaa tactattttc tacaaggata
     6301 tcacaaacat tttgcgcctg gtttgctgga acacagataa cagcaacttt aattttttct
     6361 tttttaataa attcaccaat tcctgagata tttcttatta taatattttc aatttttttc
     6421 ccgacttttt ctgggctttt atcaaaagca ccagatattt caatatttaa atcagaaaat
     6481 ccaggataac caattagagc agaacccaat ctcccaactc caataataat aaccctaatt
     6541 ttctcatcta tgcctataat tttttttatt tctcttatta actttcctat atcatatcca
     6601 acacctcttt taccaaattc accaaaaaaa gataggtctt ttctgaattg agatgatgga
     6661 atatttaata tttttgaaat atgtgaagaa gaaataattt taacaccctc tctgtatagt
     6721 ttttccaaat atcttaaata gacagccaac cttttaattg tttctgatga tatttgttgt
     6781 ttttttatca tttatgccct tgattttcca cctgatatat ttatgactgt tccagttata
     6841 taacttgcca tatcagaaag aagaaataaa accacatttg caacttcttc aagttttcca
     6901 actcttccca agggaataga aacattttca taactttttc ttaattgttc aactgttatt
     6961 cctcttgtat atgcaagtga cctttcatat tcaggtgttc ttaaagaggt tggttctagt
     7021 ataccaggtg ctatacctac cactcttata ttaaattttc ctaattcttt tgcccaactc
     7081 cttgtatatg agtataaaga tgcttttgta cccgcataaa cactctgccc ttctgaacct
     7141 tcaagtccac tttcagaaga tacatttaaa ataactcctt ttttattttt aatcatttct
     7201 cttgcgactg cctgtgcaca aaaaaatgcc cctttttgat taactgcaac catcttatca
     7261 aaaatatctt ctgtaacttc ttctttccca tcagggtcaa caagcaatct tggagtattt
     7321 atgccagcat tatttacaag agcatctatt ttcccatatt tttccttaac aatttttacc
     7381 atttcttcta tttcatcttt tttcgtcaca tccactttga caaaataaat attttctcct
     7441 ccaaactcct tttccatttc tttcccaatt ttttcattta tatctgcaat aactacaaat
     7501 gcttctgctt ctctcaattt caaagatata ctttttccaa ttcctaaaca tccaccagtt
     7561 acaattacaa cttcttcttt taatgatata ctgaccaatt ccctctcctt tttttgactt
     7621 attacttttt tatatctata atatatcagt aattataaca cttaatactt aaaaggaaaa
     7681 attatggaaa aatataaagt cccgattgag aagttaagaa aggagtgtcc atctgattat
     7741 ttcaaatttg aatccacaca acaggtaaat ccaccagaag atattattgg acaggaaaga
     7801 gcaaaaaggg cattagaatt tggtctaaaa ataaaaagta ctggatataa catatatgtt
     7861 tcaggaattc caggtacagg aaaaacgaca tctgttgagc aaataataaa actactggct
     7921 gaaaatcaac cagtgccaga tgattgggtt tatgtttata attttacatc ccccgataat
     7981 ccaaaatcac tgagatttcc accaggaaaa gcaaatattt ttaaaaaaga tatagaaggg
     8041 ctaataaatg acctgaaact tgaattttca agggtctttg aaggagaagt ttatgttgca
     8101 cataaaaacc agatacttaa ggactttcaa aataaaagcg ataaactttt tgaccaactt
     8161 gaggaatcag caaaaaaagc aggattccaa ataaaacgaa ctccatcagg aattttattc
     8221 atcccaacaa ttgagggaaa accacttaat gaagcagaaa tagaacaatt aacagagcag
     8281 tttaaagaag aaataaagaa aaaacaagaa attctttacg aacaattaga tgaaatttta
     8341 agacagataa gacaatttga aaaagaaaca aagacaagat tatcacaact tgaaaaagaa
     8401 acggcaaaat atattctgtc accaaaaata ggggaactta aagaaaaata ttctatccag
     8461 aaatatgtaa ttgattactt agatgaactt gaaaaagata ttgtcgaaaa tgttgacgaa
     8521 tttaaagaaa aaaaagaaat tgaaatacta cccgggatga aattaccaga aagagaatca
     8581 acccttttta aatatagtgt aaatgttctt attgataatt caaaaacgaa aggtgcccca
     8641 ataatagaag aaacaaatcc aacctgctat aatttgtgtg gaagaattga atatagacct
     8701 cagtttggag caatgattac tgactttaca atgataaaac caggggcttt acataaagca
     8761 aatggtggat acttaattct tcaggttttt gatgttctta aaaattattt ctcatgggaa
     8821 atattaaaaa gggcactcaa aaataaacaa ataataattg aagaccttaa tgaacaattt
     8881 cgtctaataa ataccccaac tttaagaccc gaaccaatac catttgatac aaaggtaatt
     8941 cttataggaa acccattgta ttattacctc ctttattatt atgacgaaga tatgaaaaaa
     9001 cttttcaaag taaaagcaga tttcagtaca attatggata gaaataaaga aggaatagaa
     9061 agttatacat ctttcataag caaaatctgt aatgaagaaa atttaaaacc atttgataaa
     9121 gaagcagttg caaaagtaat tgaatatggt tcaagattgg tagaagacca ggaaaagtta
     9181 acaacaagat ttattgaaat tgcagatatt ataagagaag caaatttctg ggcagaagaa
     9241 gaaaattcaa atattgtcaa aaaagaacat gtgaaaaaag cacttgaaga aaaaatatac
     9301 aggtctaatc ttatagaaaa gcgaattgaa gaactgatta aagaaggaac tataatggtt
     9361 gatgttgatg gagaaaaaat aggacagata aatggtctat ctgttatggc acttggggat
     9421 tatagttttg ggaaaccatc aagaataact gcaactattt ctgttggaag agatggagta
     9481 ataaatattg atagagaagt aaaacttgct ggaacaattc ataataaggg gtttcttatt
     9541 ataaacaatt atataaaaga aaaatttgga cagaaaaaag caattacttt ttctggcagt
     9601 atttgttttg aacaattata tgaggaaatt gagggtgata gtgcatcttc aacagaacta
     9661 tatgttttac tttcagttct ttcaggactt ccaataaaac agggtatagc agtaactggc
     9721 tctgttaatc aaaaaggaga aattcaacca ataggcggtg ttaacgaaaa aatagaggga
     9781 ttttattata cctgtaaatc aataggactt acagggaacc agggagttat aattcctgag
     9841 aaaaatgtaa aaaaccttat gcttaatgat gaagtaattg aagcagtaaa aaatgggaaa
     9901 tttcatatct ggtctgtgaa aacaattgat gaagggatag aaattttaac acgagttcca
     9961 gcagggaata tgaaagaaga tggtacatat cctgaaaaca ctgtaaatta tattgttggt
    10021 aagaaaattg atgaacttac agaaaaatat atagaaaccc aaaaaccagg aaaaagcaaa
    10081 aagaaataat tatagataca tcaaaaaatt ccatatccaa atcttctgaa aatatcaggt
    10141 ctatttctcc agtccttaat gacctttact tttagttgta aataaacatt tttcccgagt
    10201 aaaaattcaa tttcttctct tgccaattga ccaatttttc ttattctttc tccgcccttc
    10261 cctattatta ttcctttaag attatctctt tcaacaatta tattagcact aataaaaata
    10321 acatctttgt ttttctgtcc ttctcttatt tcttcaactt caactgctac ttcctgtgga
    10381 acttcctgat gtgttttttc aagaattttt tctcttatta tttcacctat tttatattca
    10441 gttggcaaat ttgtatctac atctgaagga taaaactttt ctccttctgg tagatgagaa
    10501 attataactt taataaggcg gtcaacattt tgctttttaa gagcagaaat aggaacaata
    10561 tctaaaaatt catatttttt attaagttca tcaattaaag gtagaagttc ctctttatct
    10621 tttagtaaat caattttatt tataacaagg agtatttttt tatttgtctt ttttaacact
    10681 tcaagaattt tttcatcttc ttctctccag cccttagcat caattaaaaa aagaattatg
    10741 tctgtttcat caattgaact taatatcatt ttttgcataa acttgcccaa ttgatttctc
    10801 catttttcta ttccaggagt atcaataaaa ataatttgcc cttcctcaca gttataaatt
    10861 cccaaaattt ttattcttgt cgttgccggt tttgaagaaa caatagagac ctttttattc
    10921 agtatattat ttagaagtgt tgatttacca acatttactc tcccaacaat agtaacaaat
    10981 ccacttttca ttcaggtatc tcccaatcat caattttaaa aatttctttt tttcttttta
    11041 aataataaat ataattttca taactcacat caggtggaac cctatggtca acatgaggta
    11101 tatacccacc tgaattaaca aactccttta ttctttctaa ttcattatca attgacttct
    11161 ccccattaat taaagcaatt ttattaattc cacctttaag aagaacattt ttgccgtatt
    11221 tttttcttaa aacaacaggg tcagaaccac tatttatttc aagaggaaac ataacattta
    11281 ccccagcaga aagccataaa ggaacaagtt gatttatatt tccatcacaa tcaacaatta
    11341 caacatctat ttcttctttt tttaatttag aagttatttt cttatatttt ggaaccataa
    11401 atttctcaaa aagttgtgga gatatcatcg gctgcgtctt aaatgccata tcctcccaaa
    11461 aacatgcaaa gtcaatacct ttaacttctt caagtgcctt ttcatttaat gtaagaacaa
    11521 aatttgctac atatcccatt atatcttcta taaggtctgg ctgagtataa aaacttataa
    11581 gacaattctc aaatcccatc caatccctaa cccatccaaa taaactacca acatttattc
    11641 ctaaaggata atcccttttt ccccattctt tttttaaact ttcccagcta gaaggatacc
    11701 ttttttcaat atcaggttga agtcgttctt taaactcatt ccaatcattt ctattttcaa
    11761 caggaaattt taaaaaatga ggaattgatg aagaaccatc tttaaatatc tgtaaaattc
    11821 tacctgaact atctttttta attatatgtc ttcctgtctc ttcaattatt tcttcttcaa
    11881 aataaggtag taatccaaga ttgattggaa ttatttcttg cctatcaaat ttaaaatata
    11941 tatctccttt attattgtta tttatatatt caggtagccc ttctttatgc cattttaaaa
    12001 atgtctcatt ccaccagcca aattcacagt tgaatatcct atcaacttct tcaaactcaa
    12061 aaatccttct ccatctttct ctatttgtca tattcctata aaataatttc ctgccctttt
    12121 tctgctgact ccttttcttt aagaactaca taaagagaat ctcttgcatc ctcaggggtt
    12181 actacctgaa atggtttgtt atgaataaca tggtctataa aatatcttaa ctcataaaag
    12241 taccctccca tatcttcaat atttccctct attccttcta cttttatttt ttcaaaagtt
    12301 ggtctttcaa cattcccatt tgccccataa gcaacgacag ttggagtatt tcttgaatta
    12361 aactcaattg tccctttttc aaattttgca atataactca tttcaaatgg aaaacttgga
    12421 ggaaaatccc attcaccatc agcatgagca acaatcccat ttccaaatgt aaaagttgta
    12481 agtacattag catcattatt atagttttta actatttttg aaaaaatttt tttaggtttt
    12541 tctcctaaaa gataaagcaa aaaatcagta tcatgaatat gaaggtcaac aagggcccca
    12601 ccactttttt cctgtgaaag caaccaattt tcccatcccc atgttggaag tggagataat
    12661 ctcctcattg aaattaattt taaatcccca tatcttttac tatcatatgc atctttcaag
    12721 aatttatatt caggccagaa tctaatgacc tgtgcaacca taaatttgac attattttct
    12781 ttacaaactt ttatcatttc atttgcatcc tcaacagtaa gagctatcgg tttttcacat
    12841 ataacatcct ttttattttc tgctgctttt ataacatatt ctttgtgtaa aaaagtaggc
    12901 agacaaacat caacaatctg tatatccggc tttttaagaa tttgctcagg gtcgaaatat
    12961 ggtattgttt taaactttct tgcaagtgat tttgctttct cccctcttat atcagcaatc
    13021 cctacaatct ttacatccgg caattgacta taaactgttg aatgcatttc tcccataaaa
    13081 cctgctccaa gaataccaac attagccatt tttccttcct ccttttgtaa tttttaatac
    13141 tggtaatatt ctattgccac tttattgaaa atgaaaaaac attatttttt tcaactgttt
    13201 ggactaattt tccatcaaca agcccaacag caaagtcaat tttttcctgg agttgatact
    13261 tccataaccc ctttttatca taagaaagaa agtaaaaata gttacccttt ttaccaccca
    13321 aaatatccag aacagaagga gaaaatatag caaaatcaat atcttcatca gaagaaaaat
    13381 tttttattaa agttccatca ccattaaaaa gataaatttg tccttttttt gttgaaacac
    13441 agataaaatt tgtatctgta gttattttta tatcttctat tttcccttca acattttttc
    13501 tccatctttc atttccatca tcagaaaaac aacaaacaac accattttca agtccagcaa
    13561 aaacccaatg ggcatcagag gaaattttta taatatctat atttttacta acttctaaat
    13621 cccagaaaat ttcaccattg ttatcaacat aatagacatt attatttaca ccaattacaa
    13681 gaaaccttcc atcttttgaa atatcaattc ctcttccata attgtttaat tttttattcc
    13741 ataaaacagt tccatattta tttaaaaagt agatattccc gcctaaacaa taaaaagaga
    13801 aatattgtcc atcaggggaa acagcaactt taacaatttt ctctggaaaa gataattttc
    13861 taataatatt tcctttatca gaaagaatat aaacataacc tccttcagtt cctaatattg
    13921 ttctttttcc atcatctgaa atttttatat cagatattaa actatctgct tcaaatgacc
    13981 atataagttg attttcaggt atataaagaa aaccaggaat atctttttcc tgaaacttat
    14041 aacaaaatct ttctataata tttggcaaaa atctaatatc atctgctttg aattcataat
    14101 tttcaaaaaa tatttttttc tttgggtcaa aaacatttat ttcgaaaata tattgtgatt
    14161 caattcttga aaggagtggt tttataacat aatcagcatt taaaattttt gcaacatagg
    14221 aaaggtcttc ttcatcacca atatcttctt ctgtaatgtt ttcctcttct aatacttttt
    14281 taagttgttc atttgttatt aaatttatgt tctttacttc ttccttaaat atcccaagga
    14341 ataaatcctg aaaatcactc tttaaaaaat caggtacatt attataacta acatttaaag
    14401 taagtatatt tgtatttttc agtaataatt gaatattttt tattttactc gtaagatagg
    14461 aaacagaata tttttcacca aagaaatttt ttgtctcaac tacatttcta atttcttcat
    14521 aaataccttc tgcatctctt aacatatttt ctttttgata aatagttgca agtttcatca
    14581 tataagtagt atctggaatt atacattttg aaagatacat ttgaaggtac tttataccct
    14641 ctttattttt attatgagaa agagcataat caccaattat tctgtataaa tatgcatctg
    14701 gagttgcata tttcaaatat ttcattgctt ctctatcctg gggccaacca tttggccaat
    14761 tagaaggaga aaatggtaaa acagattgta tttttgtttc tgtaagacga agataataat
    14821 cagcataaga agtcatacct tttgtatcaa attcatctgc tttttttaaa taatttttta
    14881 tgtaatcagg gaatacaaca agaatatttt tatttttctc ataccactca cttgaaaaac
    14941 aaataaaaga aaataagaaa aacaaaaata gaaatttttt cataacgact ccttaaaaag
    15001 cataatacca aattgttcag ggatatctat tctccctgtc caagttgaag ttgatctatt
    15061 ttctatttcc cttagaaaat ttatgcgcca tttatctcca ttttttattt cgtttatctc
    15121 aaattcactc aaaggaatag aaatttctgc aatccatcta tttgattcta cattactttt
    15181 agcaatccag tttgattgcc aatttttctc cttgtcatta tctgtataaa atttccagat
    15241 ataggaatat ctaattaagc ccattggatg aagaacaaat tgataccatt gccctttaga
    15301 attatacggg gctatagaaa ttgttataaa atcccttgta tttaaagtta tttctctgct
    15361 tccctggtct tcacaaatta taagaaaata taaatttttc tcatcatagc caactgcaac
    15421 ctttgtctgg atattataag ggactttttc tttttcaatg agtataaatt cattactcat
    15481 ttttaccata tcccagtatt tatcatcatc ctttccatct atattgattg taccttctat
    15541 cctatgagca tatataattt tttctgtaac ctcttgaatt ccaagttttt ctttataaag
    15601 tgcttgtctt atttgaggta ttttccccct taaagataat ggtattgaac tatcatttaa
    15661 atacctattt gcttggcttc ttaatggttc aatattttct tcagatattc taacaagaaa
    15721 ttcaattccc tttatttcac ccatttttga tagagagtaa gatgctgcaa gtttaacatc
    15781 aagttcttca tcttctaatg cctgtaaaag taatcctata cttcttttat ctttgatata
    15841 cccaaaaatt tccgctgctt ttttcctgta ttgtggatta gaactattaa taaacttcat
    15901 agcatatggg aaaacatcct tttgccccat ttttgcaaga gaataggcag aataaagttg
    15961 taattcaggg tcattgtgtt ttaaattttt ttgaagaatt ggtattgctg tcttatctcc
    16021 aatttctcca attgcaagtg tagatatctt tcttgtttca atatctgagt ccaataatgc
    16081 atcctttatt cctttggcaa aaaatctttc tggttttctc agaacaactt caattgcttt
    16141 atttttaaca tcagaataat tactcaaaag cattttataa attaaatgtt taacttcgtc
    16201 tgtttgaata attgcatcca ctatatcaat tacaaatttt ctatcacttg ggttagaact
    16261 tttaattttt tcatttaatt tattcacaac atctcttaaa atttttctgc tttcttcttt
    16321 tactcttcca catgtctcat ttttttcatg ataagaaagt atttctaaat attgaacatc
    16381 aaacatgtcc aaaagtgaaa atgcaacaat tgccccatat cttattaact ggttagggtc
    16441 ttttgttttc tcaataagat aattttttac actttcatct ccaactttac caattaaatt
    16501 caaagccgat agacgcgaat ttattgaatt gttttcatca tcaacaaatt tttcaagtgc
    16561 aggaataaaa gcagtgtctt ttatattttc aataattttt attgcatatt ccattacact
    16621 ttgtgaatta tcaccaagtg catttacaat ttcaggataa gttgatacat ttctaaactt
    16681 ttcaagagcc cataaagaat atattcttac cgattcatca gggtcattta atccttttaa
    16741 tattccttct aatccttctt ctgtcttcaa tctaacaagc gtttttatac tttctatttt
    16801 tacttttacc gatggggcag caagcaatga ttcaatttta tctgctgcat ctgtcgcttt
    16861 taacttctca agagcccata cacttcttat tttaacctca tcattttcat catctaacgc
    16921 atttgttaaa taagaaatac cacgactatc ttctgtactt tcaataaggt atattgtcct
    16981 tattttaaga aatacattat tgcttttaag caaattataa atattttgaa catcagaatc
    17041 aggaagattt ttaaatttct ctctattttc tgccttccac atcaatcttg ttaatgacca
    17101 gagaatttcc tcttttactt tttctttatt atcatttatc tttctaacaa gaacatcaat
    17161 agagtcagaa ttcccatatt taccaattcc agaagcagca taatatctta cctgccagta
    17221 agagtcatca agtgctttat gaaactgcaa tatttcttct ccagaagcaa attctacaag
    17281 acctcttatt gaataatatc ttacctgcca ataactatct tctaatccct ttttatagta
    17341 atcaacacag gttgaatcac ctattttcca taaggtatat gctactttac tcctaacgat
    17401 ttcatcttta tcatcaagta attttgcaat atcaggaagg tatgttttat cacctttact
    17461 taaaacttta ttaataaaat ttattttatc ggaagaagtg ccatacttaa ccacatttgt
    17521 atctaattca gcagaaaaaa gataaaaact aaaaaaaata aataaaaaac caataaataa
    17581 tttatttgtt ttcatagtaa aattttggaa atccaatatc aacaattttt attttaccac
    17641 aataagcagg accttcattc atataaaaac cttttttccc aaaacctaag gatacagtta
    17701 aattaccttt tactgccact ccacagacat ctccggtatc ggcattcaat ccagatggaa
    17761 catcaacaca aattttataa gcaacttttt catttatata ttctactatt tctcttacct
    17821 ggtcatttac cattccacta atgcctgttc caagaattgc atctataatt aaatcacatt
    17881 ttttgtattt taacacactt ttaaatttat tttcaactga aaaaatttta actcccaaat
    17941 ttttcaaaat tacaaaattt ctttttgtaa tttccgaaaa tttattttct tctggaatat
    18001 aaaaaacatc aacatcaaat cccccattaa aaagatacct tgcagtaaca aatccatctc
    18061 caccattatt tcctttccca caaaaaattt caatttttct ctttttattt ttaataattt
    18121 tttttgtttc ctcacttaca catctaccag cattttccat taaaattagt tttattatac
    18181 catacttttt ttctgtttca tcttctattt tttttatctc ttctgatgaa agaactattt
    18241 ttttcatttc aattacccct taatttttct aaaagttctc tcccaaccaa atttacagga
    18301 ccagccccaa gagtaagtac tatatcattt acttttaaaa cttcaagtaa gtactcaaca
    18361 atttcttcaa aagttggtaa ataaagtgct tctttaccat tttttctgat tttttcaact
    18421 aaaatttctg catttacaag ttttttttct tttaaagaat ctctaacaaa ataaatatct
    18481 ggaacaacta tcttatctgc attagaaaaa gagcgggcaa aatcctttaa aagaaatctt
    18541 gttctactat attgatgtgg ttgaaaaaca acaatcaatc ttctcccagg aaaaactgtt
    18601 tttaaagtag agagagttgc ttttatttca gtcggatgat gcccataatc atcaataatt
    18661 aaaatccctt tttcattgcc tattatttca catcttctat gaactccatc aaatgtttcc
    18721 actgcttttt ttatatctcc aaatggtaat ccaataatat tccctactgc aatacaggca
    18781 agtgaattta aaacattatg ttttccccat attttcaatt tcaaatgtga tatttttttt
    18841 cctttaaaat aacaatcaaa ttcagttctt ccttttttta ttacaatatt tctccctgta
    18901 aaatcaccat tttcaaaacc ataagatatt ttttctctct taactttttt aaccacttcc
    18961 acaacatttt tatcctcatt acaataaact ataaaaccat cttttttgac attattacag
    19021 aactcccaaa aagcactttt tatttctctt atatcataat aataatcaag atggtctttc
    19081 tcaatatttg ttaaaacagc aatttctggg gaataattta gaaatgacct tttatattca
    19141 caaccttcaa caacaagaaa atcacttttt ccaaccccag aatttcccag tccaataatt
    19201 ttccctccca caagaaacga aggagatagt ttattttttt taagcaagtg gacaacaaga
    19261 gcagatgttg ttgtttttcc atgcgtccct gaaatagtaa tgcctctttt atttttcatt
    19321 attgccccaa caccttctgg ataactcatc atagggattt taagttgttt ccctttttta
    19381 tattctggat tatctggaaa aattgcttga gaatagataa ttagttcaat gtcctttgtt
    19441 atattttctt ctctctgtgg agaaaatatt tttacccctt ttttttcaag tgcttttgtt
    19501 ataggggtta aatttgtatc agaaccagaa ataattttcc cctctttaag caaaatatat
    19561 gcaagtccac taacccctat ccctccaatt ccaataatat gaactctttt taccctttca
    19621 aaaatatcat tttttctcat ttagagcacc agttataata ttaacaattt ttccatcatc
    19681 ataaatcttt actttttcaa gtgccttttt tattttctca taatttccca aaaatttctc
    19741 aaaatataat ggaaaattat aaacaatttc ttccctttgt ggaataagaa aacaggcatc
    19801 cttttcctgt aaaaacattg cattaaaaaa ttggtgtccg ccagcaaaag gatatggaat
    19861 taatatagaa ggtattttcc agtatgtaag ttcagaaatt gttcctgccc ctgctcttga
    19921 aataacaata tctgcaatag aatatagttc attcatatta taataaaaat caaaaacaat
    19981 cccacttact ttctttttaa aaaaaatctc ttcaactttt accttttctt tacctgcaat
    20041 aattataata tgaaaatcgt tttttttaac aaaataatca atattagaag ttataagttt
    20101 atttaaaaaa gatgctgact gacttccacc aaaaacaaca attacttttt tatttttatc
    20161 taaaaaaaaa ttttcaatta tttcttcttt tgagatatga attttaaatt cctttaataa
    20221 agggaaacca gtaacaacga ctttcttttc tggtaattta tttctataag gaaaagttaa
    20281 aaaagttctt cttgaaagaa ttgaaaaaaa ttttgttact tttcccggga tataattttg
    20341 ttcgtgaagg tatatatctt ttcttaaaat tttcccagca acaagaaacg gaaaagttgt
    20401 ataactgcca gtgccaacaa ttgcaacaaa attttccttt tgaataaaaa taaaacattt
    20461 aaaaatattg aataaaaatt taaagggtat tataaaaatc cctttttttg aaatttttac
    20521 ctcaggaata atagaaaagt tataattcct tttatttaac caattaatta taaatcccct
    20581 ttcaggaaca aaaaatttaa cttttatccc tttttcaatt agtgaatcac ctattgaaag
    20641 agcaggaaaa aaatgtccac cagttggtcc tgaaacaata gcaatttttt tttctttatt
    20701 atgcaataat tctctcctta tttcccatat tataacacat caaccgcata aatttaactt
    20761 tcccagacat acttacttgg ctaaaaagtt gattacatta ttattttata ggataatttt
    20821 gaataaatgg aattataaga gaaaaaataa ggaatatcat aggaggtggg gaaatgttaa
    20881 aaaaacttgc taatgaggaa ataaattggt tattactttc tgaaaaagaa aactacgaaa
    20941 aaaacattaa aataattgct cagaaaaaca aaataggaga aggagaaatt acagaagaac
    21001 tgaaaacaac aactgaactt ttagatatag gagaaagaat agagaacaaa ataataataa
    21061 ctcctgaact atttaaaaat gtatttctac ttgccctgac agattttgaa agtaaattgg
    21121 taaaatattg ggatacccct ggtgaattca caaaaataat gattggtaaa acactatcag
    21181 acaaaagcat gttgcattta atggcagata tattgaaatt aaaatattat agagaatact
    21241 ggtcattaga tgctgttttt tatgaaaaag aagacaaaga gcattttcag ttgccatttt
    21301 tggttgctga atatttgcct gtggttattg aacatgaaaa cgcaatatct ggaagttgtc
    21361 ttgaaatgaa taaattatct atattcaact ccccattaaa agtccttatt acttatccag
    21421 aaacggaaga taaaaataaa taccttaact ggtacacaga aatactcaaa aaagcagata
    21481 tttttgatga tttttcaaca aaaagaaaac atcttttaat ttttggatat aaaaaagaag
    21541 agaaaacatt ctgggagttt tatacctatc aaaataaaac attttctcta cttgaataaa
    21601 aggtaagttc cactgaatcc aattagggca gacataattt tggggtatat aaatatctta
    21661 ttgcttaata aatatggcta actgaacttg aagaaaaatg taggtctttc gtattgaagt
    21721 tgcatttaaa gtatttaata tatcatcttc aaaaaacaat tttttctcta ccagtgccag
    21781 aaaaactttt cttgtttcct ttgtaaacca atccaaaatc ccgttttctt ataactatga
    21841 agaattgaaa attatcaaaa acaaaaagta gtgtagattt agaattgtga atttgaataa
    21901 tagatgtatt tatattataa ctatgaaaaa atttaaagaa ttcacatttt gaaatggggt
    21961 aatactattt ttacattaaa ggtgtaataa aaatcagagc cctcacccct gccctctccc
    22021 ccgactatcc cttacccata agttccccct tttaaaaaat gttaagatag aagaacatgg
    22081 ttttctatga gaaccgggtt cttgaaacaa taaggtaagt aagttcctca tttaacaaac
    22141 cgacaaaagt agcaggactt agcagggcag agttggtgcg ttatcgtctt ccagatagca
    22201 caacggggat gaagtggaac ttaaagggat ttccaagcac aacgggacaa agtaagatgc
    22261 agagaaattc aaccccgggc gttgcccaaa aaaattttgt gggaagttag agtattacaa
    22321 agttttaata aaagacaacc agatgaaaga tacttttcaa tcatatgatg ttcatgctta
    22381 tataatcaaa atgaaataaa aagaagttag aaaagagaag ttaaaaaaga tatctcttta
    22441 ctgaaagtgg atatttcacc atgaccagga tatattattg ttttttctgg tagtttaagc
    22501 aatttcttaa ttgaatttaa aagttcttcc tcatctcctc caacaaagtc agttcttcca
    22561 attccaccag cgaaaattgt atcccctgta aaagcaaaat tttctcctaa aagagatata
    22621 cttcccggtg tatgacctgg tgtatgtaaa acatttaaag aaatattgcc aatttttatt
    22681 atatctcctt cctgcaatat ttttgtctgg caattacata taaatggtat cccaaaaagt
    22741 attgaaagat ttttttcagg gtttttaaga tattcttcat ctaatttatg aatataaaca
    22801 ggtaaagaaa atttttcatt ttctccaata tggtcaaaat gtccatgtgt atttattatt
    22861 atttctactt ttattttctc tttttctatc tgatatttta ttttttcact ttctgcccca
    22921 gggtctataa tagcaccttt tcctgtttct tcacaccaga ttatatagca atttgtaaaa
    22981 aattctccaa caacaatttt ttttatcttc attagcatat ccttggaaat tgagcacctg
    23041 cctggaactc aacaactctt tcaactccaa tttctgtctc tattaaaact tttcttttta
    23101 ttttgtctgt tacttctcct ataatttctg ctttttttcc caattgttgt tttctcattg
    23161 tttcaaccac cttttctcca tattttctat caactataat aattatttta ccttcatttg
    23221 ctatgtaaag agggtcaaaa ccgagaattt cacataagga attaacttct tcacttatgg
    23281 gaattttttt cttataaatt tttattccaa tatctgattt tataactatt tcatttaagg
    23341 ttgccgcaac accacctctt gttgggtctc tcataaattt tattttttct ccaaatggaa
    23401 atatctcttt cacaatagga tataaagaag aacaatcact tttaattgaa ctttcaaatt
    23461 caataccttc tctctttgtt ataatagaaa gcccatgtaa tccaatatct ccatttatta
    23521 ttaccaggtc tccaagtttt attctttcaa ttcctaattt catcctttta tcttttattc
    23581 caattccacc tgtatttata aagattttat caactttccc tttttcaact acttttgtat
    23641 caccagtggt tatttttaca ttttcttttt tactttcatt tgccattgaa ataactattt
    23701 tttcaagtgt ttcaaaatca aacccttctt ctattataaa tgatgaagaa agaaaaagag
    23761 gggttgcccc acttactgat aggtcattta ttgttccaca gattgaaagt tttccaatat
    23821 ctccaccagg gaaaaaaata ggacttacag tataactatc tgttgtaaaa caaatatctt
    23881 ctttcccaat ttttaataaa gaagcatctt ggagtttttc aagatattca tttttaaaat
    23941 gttttacaaa aatattttct ataagattat aagttaattt tccgccatct ccataagata
    24001 aagttatttt ttgcattttc ccctaaatgt ttgttaattt ccgtttatat ttttaggtac
    24061 taattttaaa aattcccctt caaatccccc cttacccccc tttgaaaaag tggggaataa
    24121 cgaaccaact tactccctcc tttaaaaaag gagggatggg ctcgcccgta gggtcctcac
    24181 ttttggctac ggattttaaa ctatgcccga gggggtggat ttagaaaaat ctccccttgc
    24241 ccctctttta taaagagggg aatattaaat cccccctcat ccccaccttc tccccagcgg
    24301 ggagaaggat gaaatccccc tttttcaaag ggggaggtat tttggaaata aaacaccaac
    24361 tctgccctgc taccctttct ttaatgtgga ggaccgtcct ccacagaata actatgttga
    24421 ttgaagagga atctcatatt tataataagc agaacatgtc ccttctgatg ataccatgca
    24481 tggtcctact ggattttctg gattacaaaa tttggcaaat aatttacaat caaatggctt
    24541 ttttgttcct ttaagtattt ctccgcatat acaggaactt tcttttttta tttcaggtat
    24601 ttttacttca aatttttttt cagcatcaaa ttcatcaaag tcatttttta ttttaagtcc
    24661 acttttttct atattcccaa gtcctctcca ctctgaatca atttcttcaa aaacatcttc
    24721 catatttttt ctggcaattc tatttccatc ctcttttact gctcttttat actgaattat
    24781 aacttcacct ttttgttttg acataatttt ttcaattaaa atataaatac tctcaactat
    24841 atctaacggt tcaaatccag taataacaca tggaatatta taattctttg atataaaatt
    24901 gtatggcttc ttacctatta ttgtgctgac atgccccgga catataaaac catcaatttt
    24961 aacttcatca tcttttaaga gtaattccat tgcaggtgga ataagtttat gtcctgaaaa
    25021 aacataaaaa ttttttattt tttttctttt tgctatttta atagtagaag atacaattgg
    25081 ggatgttgtt tcaaacccaa ttccaagaaa tattatattt ttatcagtcc ttttttcagc
    25141 aatttcaaga gcatcaaaag gagaatatac aacttttatt tctctacctt tgcttttttc
    25201 tttttcaaga gaagaataag agccaggaac tctcatcata tctccaaaag tagtaataat
    25261 tgtgttttca attttagaaa attctattgc tttatctata tatgaatttg gactaacaca
    25321 tacagggcac cccggtccag aaagtaaatt tatgttttct ggtaaaacac ctcttatccc
    25381 aaatttccct attaccatcg tatgagttcc acatacctcc attaaattta tatttttatc
    25441 atatttttct gtcttttttt ctatcagttt caatattttt tttattattt ccgggtttct
    25501 atactcctgt atatatttca tttgcccatc tcacttaata atttcaatgt tttccctgct
    25561 tgttttttat caagttttgt tattgcaaaa ccagcatgaa caattaaata attacccttt
    25621 ttaacacttt taacaagaga aatatttacc tttttcttaa ctgtaccaag agaaacaatg
    25681 gcaaaattat ctttcattac tttttcaact ttcattggaa ctgctaaaca cattgttttt
    25741 ctcctttcaa taaaaaatta cctataactg cctgcccaag acaaattcca ccatcatttg
    25801 ttggaacttt tctatgaata aataattcaa acccatcttt ttttaaatca ttatatgttt
    25861 tttcaagtaa aaaattgttt tgaaagacac cacctgataa agcaacttta tttatttttc
    25921 tttcttgcct tatgatattt acaactttcc ttattatatc agaaattgtt atatgaaacc
    25981 tatatgaaat aaaaccaact gtttttttat caatcaggtc atgaacaatt tcctcaatca
    26041 ttttttcaac attgataata taaacattat tttctttctt gatttgataa ttatatggtt
    26101 cactttttgt tttttcaatt ttcatttcaa gttcaattgc tgcctgacct tgataattta
    26161 ttgtatttct tattgaacat attgaagaaa cagcatcaaa aagtcgtccc atacttgaag
    26221 ttaaagggca atttatcctt ttttcaatca ttttttcaat tattttccat ttttttatat
    26281 caatattctt tacaaaatca atatcaagag aaaggaattt attaccaaat gttttgtata
    26341 aataaataca acccaaacgc catatttcct ttattgcctt ttctccacca ggtaaaggaa
    26401 tataatcaag atgagagacc ctttcaaatt cagtataatc acatattaga aattcccccc
    26461 cccagatgtt tttgtctgtt ccaaatccag ttccatcaaa agcaattcct attatttttt
    26521 catttttaag cttattttca gccataacag atgcaatatg agcatgatga tgctggacgc
    26581 caattccaat tatatctttg ttttcttcaa tcatacttac tgcaatttta gatgagaaat
    26641 attgaggatg aatatcataa catataactt ctggttttat ttcaagagtt ttttcaagaa
    26701 gttttattga ccttttatat gagtcaattg aagatacatt tccaatgtct ccaatatgct
    26761 gactgggata aactttgttt tcttttgtta aagcaaaagt attactcata tcagaaccag
    26821 cagaaaatat atttttttta aatttatacg ggaattcaat catttcagga acatagcctc
    26881 ttgaacgcct tatgaaatat gaacttcctt tgaatacctt caataaagaa tcatcacaat
    26941 ttatataaat ttccctattg tgagtaagaa aaaagtcaaa aattcctttt agtttctcaa
    27001 atgcctgttt atcatcataa acaattggtt cttctgacaa atttccactt gtcataacaa
    27061 gaacttttaa atttttattt aaaaagaaaa gaatatgatg gatgggtgta tatggtagca
    27121 taaagcccaa ataattattt gaaggggcaa ctattgaagg aattttatta ttttttttct
    27181 tcaaaaggac tattggactt tgccatgaat taagatatcc tttttcttct ttactgatat
    27241 agcaaaattc ttcaattgtt tttatatccc ttgccataat ggcaaatggt ttatcaattc
    27301 tatttttcct ctccctcaat gtttcaacaa cttcttcatt taaagcatta catgcaagat
    27361 gataaccacc aattccttta atagcaacaa tttttccttt ttcaataaac caagaggtat
    27421 tttctatact ttttatcccc tcttctacta tttctccttt tttatttttt aaaaacatac
    27481 caggtccaca atcaaaacaa caatttggtt gggtatgaaa tcttctactt tcagggttat
    27541 tatattcttg ttgacatttt tcacacataa taaattcaga cattgttgta ttttttctat
    27601 cataaggaat attttttatt attgtaaatc ttggaccaca attagtgcag ttaataaatg
    27661 ggaaaagata ccttctatta ctcgggtcaa aaagttcttt taaacaatca ggacaagtag
    27721 caatatcagg tgaaatttct acttttaggg atttttgttt tttactctca attattctga
    27781 agtttttttc attttcaata cttaaaggaa taacttttat cttttttatt ttagcccttg
    27841 gtggaggata atttttgatt ttcttgagaa aattttttat atttttttga ggtccttcaa
    27901 tttctatata aacaccttcg gatgtattac ggacaaaacc agaaaggttt tcttcctttg
    27961 catacctgta aatagtgggt ctgaaaccaa caccctgaac tattccggat attttcaaac
    28021 aaactctttc atttttattt tttaataatt tcacagaatt ttcttcctgc atcaaaaagt
    28081 ttctcttcca tatttttatc tggcttaccc tttataacaa ttgaatccat aactttttta
    28141 aagttaaatg tctgagcaat gttttcaata ctttcgatac ctttacctcc tccaccatgt
    28201 gtaagaaatg caaaatatgg ttttccttca accttgcctc ttgatggata aaatgttcta
    28261 tcaaaaaaat ctttaagtac tcctcccatg taaccaaaat aatctcctga accaaaaatt
    28321 atcccatcgg ctgaaagtaa atcatcaata tctgcctgaa atcctttttt tataacaact
    28381 tcaacatctt cattgctttt tatccctttt tttattgcaa aagcagcagc ttcggtatta
    28441 cctcctaatg aacaataaac aataagaatt tttttcattt tgcatccttt ttgatatttc
    28501 ataggttaat aatttttttt atattttttt cagttatctt ttcataaaca ctttgagtaa
    28561 tttttttatc ttcacgagat tttttaatgt attgaataat tggaacatcc tggtttacat
    28621 ggcagatatc agttccaaaa aaaagtttat tttgaaattt ttcaaggaat ttatatccaa
    28681 aatttacatc tcttgttatt gcattataac cactaccagc agataaatca gcaaaagaat
    28741 tactataatt agaaagaagt tttattactg ctccttcatt ttttatttct ccttttggat
    28801 aactactaaa ttcttctttc ttaatatctg acgagatttc tgcccagaaa tgtggaccat
    28861 gaccaataaa taatgtctct gggaatttct ttatcatttg ttcaaattta ggcaatccaa
    28921 cctcatcaag acatgttgtt ccagaaaggt caaaaataat tggcattttc aattcaccac
    28981 aaacttcata tatcttttgc atacgaacat catcaacata aagcccacac aacacttcac
    29041 caaatcctat acatcctctt tctttatatt cttttattac atttggaact tctctccttc
    29101 tggggtcaac attacaaaag ggaataaatc tatcaggata ttttttacat atttcaagta
    29161 catattctgt tgttacataa taagaagtat cctctggatt ttcaatagga agaagtactg
    29221 ctttttcaat attattttta tccataaaat ctaataaata ctctggttca agtggtggtt
    29281 caaaatataa tctgccaata tgcatatgaa tatctatcat tttttctc
//
LOCUS       DAOOVW010000021        26827 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_1217_length_26827_cov_7.637046, whole genome shotgun sequence.
ACCESSION   DAOOVW010000021 DAOOVW010000000
VERSION     DAOOVW010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 26827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26827
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1217_length_26827_cov_7.637046"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1438
                     /locus_tag="PLW95_04215"
     CDS             <1..1438
                     /locus_tag="PLW95_04215"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOV21868.1"
                     /translation="IIEFHLYFLYNNKKGEKMVKTIEEKCRLLNYQLYLLHTISKTIK
                     ETFNENRILNVILTGLTANGAIGLSRAAVFYYDRDKNIIYGRKGIGPFDNEEAYKIWS
                     ELSKKNISLEEYLSYNLEKELATQKFPQTIRKIVINLDELNEENYFKRVIKEKQIFHM
                     YMDNKQASIYLPKEIETIFIPSEIVLLPLYSSKDIIGIIMADNAFHFHPIDESTLLLI
                     SLISIQTGIALENANNYNIVQKQLEELKELHSAMESMQEELVKKEKLSTIGKMASYFI
                     HEIKNPLATAGGFVKRILESNDISSIHRDADIVLKEIKKLEQILNKFSNFTLLTPMKI
                     EKISLLDIVKEILDTFELELSKKYIDVQIDIQQNISLKAEKVQLYEILFNLISNAIEN
                     MERGTIKISAEVENSLLKISVEDTGKGIPKENIPYVIEPFFSTKNEGLGLGLFIVSNL
                     VESYGGKLEIESVEKKGTKVKIYFPIIN"
     gene            1453..1815
                     /locus_tag="PLW95_04220"
     CDS             1453..1815
                     /locus_tag="PLW95_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV21869.1"
                     /translation="MIKKKILIVEDVINEQILYKEELEKEGFEVSCVSNGIEAIEFVE
                     KNLCDLVILDLHMPKMDGIETLGKLLTKKRNLPIVIYTAYPQYREQFLTWAADAYLIK
                     NSDIDLLKNKVKDILSEK"
     gene            1812..3026
                     /gene="glgC"
                     /locus_tag="PLW95_04225"
     CDS             1812..3026
                     /gene="glgC"
                     /locus_tag="PLW95_04225"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009098308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOV21870.1"
                     /translation="MNLKDITIFILAGGKGERLYPLTRDRTKPAVPFGGIYRIIDFPL
                     SNAINSGLRKIYIFTQYKSLSLHRHIREGWNIFSRELGEFIDFVPAQQRIGDEWYQGT
                     ADTVFQNINILELEKPKLILILSGDQVYKMDYMRMINEHLKKKADMTLATFEVPLNEA
                     HRFGVLSVDKDNRIIEFKEKPNNPFPSPENPEVALISMGIYIFNLDVLIKKIIEDAKK
                     SESSHDFGKDIIPSMVGKDNIYAFRFIDENKKNTKYWRDIGTIDSYWEANMDLVNVDP
                     LLNLYDRDWPIRTFQEQYPPAKTVFSTGDRKASVTDTMLSSGCIVSGSKVIHSVLSSN
                     VRVHSYAEVIDSILFENVNIGRHCKIKRTIIDKNVDIPPHTEIGYNLEDARKRFHVSE
                     NGIVVIPKNYKW"
     gene            3034..3375
                     /locus_tag="PLW95_04230"
                     /pseudo
     CDS             3034..3375
                     /locus_tag="PLW95_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546259.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
     assembly_gap    3425..3434
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3435..4132
                     /locus_tag="PLW95_04235"
     CDS             <3435..4132
                     /locus_tag="PLW95_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015920093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4931 domain-containing protein"
                     /protein_id="HOV21871.1"
                     /translation="ANKENIYIKDYSIKTMTNIIETYQSRCIDLKKDIRFRYVLVFKN
                     FGKEAGASLSHPHSQIIATPIVPKNIKEKLFGAKWYFEYKERCVWCDIINQEIDSGKR
                     VVEQNSAFIVIAPFASRFPFELSILPLRHSPDFESIDSKEKELLAEIMVSTFKKISKG
                     LSNPSYNFMILTAPARYSRPGYWQTLEKDYHWHIEIIPRIVKQGGFEWGTGFYINPTS
                     PEEVTDFLKSLNV"
     gene            4142..5572
                     /locus_tag="PLW95_04240"
     CDS             4142..5572
                     /locus_tag="PLW95_04240"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOV21872.1"
                     /translation="MEKKNICLVAAEGVPFVKVGGMADVVGALYKYLKSKHNLYLFLP
                     FYKKIKESYKTEKIGTYDVFFSNDRQEKGFVNKSIDFPGIYFIGNDNYFERDDVYGPD
                     GKDYPDSAERFSFFSKSVLEVMKKFKINVHIFHCHDWHTALIPLYLKLYYKDIFPIAK
                     TLFTIHNLGYQGVFPVDKFHILGLPWEYFNMDELEFYGNVNFMKSGIIHSEKINTVSP
                     KYAKEILTPEFGYNLDGLLRKYQNKLTGILNGIDYQIWNPAIDGNIYKKYRGYKNKLE
                     NKLALQKRLKIQQGKEIPVFGMISRLVEQKGIDQLLEIMESILSLPLEIVILGDGEQK
                     YKEALSKWQQDFPKKLSITFAFDDKLAHQIYAGSDFFLMPSKFEPCGLGQMISFKYGT
                     VPIVRKVGGLADTVSNYNFETEEGTGFTFEGGAKEFFLCIENALKVFADKNKMERLVN
                     KIMKLNFSWKTSIEKYLKIYEELMEQ"
     gene            5569..7557
                     /locus_tag="PLW95_04245"
     CDS             5569..7557
                     /locus_tag="PLW95_04245"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HOV21873.1"
                     /translation="MIYLSILWHMHQPSYVFNGEINTIIPVFRTLFNYYPMAVLTEKY
                     PDIKINFNFTPVLLDQIQGITSAKYYDNFLSILVDDTNDEEKILTFASEIPGRILEKQ
                     YTLKILLDKVKTNTFSVQDISDIKVHLHLACFHPFIIDEELKQLVKKSRYFDKKEKEL
                     LYNKEKNILKETINKYKNLMKTGQCEIITSPMTHPIMPLLYNTNIAKQTKTSLPIPEN
                     LFSYPEDAEEQLIRGINIFKTTFEKCPDGIWPPEGSLSNEILDLFAKHNIKWTATDQQ
                     LLSETLSRPLFQKEHYNIWDFREKIFIFFRDHHLSDMIGFSYQHMEEKEAAVNFIEHI
                     HKSSDKTKNQLITIILDGENPWDYYPSFGFYFLPALYTMLSESSDIKTITFSEALKLD
                     IKHEKLERIVPGSWMGVNFDNWIGKQPANTAWKILQQVRKLSEEKINSLDDTKKEKLK
                     EYIMLAESSDWFWWYSIPADKKIKRKFDAYFRNNIRKIYEILDVKAPEFLNFPVENYD
                     IEEFFPYIKPSIDGKITHFYEWYNAVEIDAADLWTTFKPVEIPIKKIFYGYDDENVYI
                     RIDTLEKQNIEIEITFHNSVKQKFYIKNDLSSSLPISFVYGDIIEIKIPKKEILLEGE
                     KTIFFNISIRDREGHEIVLPTGDYFKITFLSEEENWII"
     gene            7545..9461
                     /gene="glgB"
                     /locus_tag="PLW95_04250"
     CDS             7545..9461
                     /gene="glgB"
                     /locus_tag="PLW95_04250"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020564309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HOV21874.1"
                     /translation="MDNIEKNQPPNRISLFGPLDLYLFNEGNHFEIYKKLGAHKRVVN
                     GIEGYNFAVWAPNAVKVNVKGNFNNWDGSKHQMVELGNSGIWEIFIPEVKECYPYKFE
                     IHTKNNNVLLKSDPYGSFFELRPANASLTYKSHYKFENSKIQPTNIYKQPMAIYEMHL
                     GSWKRRKDGFFLNYREIAEALVPYLKETGFNWVEFMPISEHPLDSSWGYQTTGFFAPT
                     SRYGTPDDLCFLIDQLHKNNIGIILDWTPAHFPKDDFGLYLFDGTHLYEHYFPQKRDH
                     PDWKTAIFNYGRYEVSNFLINSALNWIENYKIDGLRIDAVASMLYLDYSRKNGEWIPN
                     KYGGKENLEAIDFLKKLNSTIYQKHPNTFTIAEESTAWPMVSRPVHMGGLGFGFKWNM
                     GWMHDILYYFSLDPIYRKYHHNCLTFGLLYAFSENFVLPISHDEVVYGKKSLLSKMPG
                     DTWQKFANLRTLLTYMYCHPGKKLLFMGSEFGQLSEWNYNGELEWWLLEKEEHRSIKK
                     FVSDLNTLYKNEKALFEIDFENNGFEWIDFSDYEKSIISFIRKGEKENNFIITVFNFT
                     PVPRYNYRIGVPLKIHYKEVFNSDSKFYGGSNTGNFGMVESENIPYHNRPFSVNLTIP
                     PLSGIILKPHIDSF"
     gene            9510..10832
                     /locus_tag="PLW95_04255"
     CDS             9510..10832
                     /locus_tag="PLW95_04255"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOV21875.1"
                     /translation="MKKIIICVFLVSSLTLFGEVKTEVSTSGPSVSEVLQYQGPKARI
                     SVGKFEVKAAKGAWEIGDGLKEMLVDALFKTGRFIVLESSENIDTLKEEFELGESGWS
                     NKAPEKGTFETADIILTGAITAFEPDYKGKGGGGVVVPLPFAVGGGLKISKQESYIAS
                     NLRLVDVRTRRIIKTAKVEGFASKSDIGVLGGGLIGSVALGAGFQKYKNTPMEQAVMI
                     MLENAVNEIIKSVPEDYYRYDEKGNAITANQQTATASQQTTEETKIKIVGGGDIFKAG
                     EAIILNEDFNKYEIGDIPTDFQYEKAEVEIAEMNEKKWMRFLKQGNIKKDIKVEGDYS
                     YEISFLTTFNCSTDIVLPDGKSIHLTNGKILYSENELSPISINKVNKIQLSKKEGITR
                     IFVNDKRVYKAPEKLDTLSNGIVIKCDNVSPDDGKEFLFTDLKIATYK"
     gene            10829..11563
                     /locus_tag="PLW95_04260"
     CDS             10829..11563
                     /locus_tag="PLW95_04260"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOV21876.1"
                     /translation="MIIGIDCGNSYIKIGLFKEKIIKQVVKFPVEDINKFRIPYHWKK
                     LNIEVIALASVSPINSDKIKTTFSSFFNKKVITITSQQCNIPLNIKDKKKVGVDRVLN
                     CKGASVLYGNPSIVIDIGTAITIDFVNRNGVFEGGIIFPGPFLLLNSLNKTSLIKSPN
                     FLKKNILIGKNTDECITSGLNNGIVGCIENIVGKIKNIHKNSNVILTGGWCDYFAKKI
                     KIKKVVKKNLTLEGINIVIRERWLKK"
     gene            11548..12102
                     /locus_tag="PLW95_04265"
     CDS             11548..12102
                     /locus_tag="PLW95_04265"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOV21877.1"
                     /translation="MVEEIIAGEKVYFYDEVSSTMDVAKKLLKEGEKGIVVAKKQTKG
                     RGRYGKYWLSDEGGLYFSMIIEKSVISDFLSEIISISLVETMQKFGIICKIKFPNDII
                     YKGKKISGILIERSDDNYIVGIGVNINNKISEFGISMKEILKKEININEFLEKFLTIY
                     KTNEIKFCENKENCLKEWSEFLLK"
     gene            12099..13361
                     /locus_tag="PLW95_04270"
     CDS             12099..13361
                     /locus_tag="PLW95_04270"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOV21878.1"
                     /translation="MSQPDIFQNLIFIFILVCLSAFFSASEAAFISLNKYHIQKFSDI
                     NKEKASPIEFWIKYPEKILITILVGNNIVNILVSIIAAITSEKLFHRTSIALITGIMI
                     FIILVFGEIIPKSIGKRHCEKFVRISFYPIKFLSILLNPVIKFLIFVANFFVLPFGQR
                     INTIMPVITKEDVKAMIEAGEEEGVIEEEEKEMIHSIFEIGDKMVKEIMIPRVKIVGV
                     EENTKITDVIKVIEKEGYSRIPVYKNNIDKIVGIVIIKDIVAQLNKEGSTELMAKDIM
                     RDVFFVPKTKRVLELLRDLQNKKLQMAIVVDEYGGTSGLITMEDLVEEIVGEISDEYK
                     KDTRPYHKLPDGSFLVQGSMEIEKFEEIFDFKKDEDSDVETIAGFILDYLGKFPKTGE
                     KFTYNNFEFTIQESDEKNIKWIKVKKIV"
     gene            13607..15022
                     /gene="lysS"
                     /locus_tag="PLW95_04275"
     CDS             13607..15022
                     /gene="lysS"
                     /locus_tag="PLW95_04275"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOV21879.1"
                     /translation="MAQDEIRDQRIKKIEILKSRNIEPYGRRYEKTKTEDIGEQGEVK
                     TAGRIIALRKHGGACFADIIDTEGKIQLYFKKNIIGEELYEIFKEIDIGDIIGVEGEI
                     FKTKTGEKTIFVKNFKVLSKSLNPLPEKWHGLKDIEIRFRKRYLDLLINKEVREKFIK
                     RTKIIQFIREYLNNMDFIEVETPMMHPIPGGAEAKPFITYHNTLEMNLYLRIAPELYL
                     KRLLVGNFEKIYEINRSFRNEGISTLHNPEFTMLELYSAYGDYEEMMEITEKLLTNLV
                     NFLYKSSTITYQDKEINFSLPWEKITYDDVFKKVGIENWHDINSVKEKIKEFNLEVEE
                     EKENNLFELLDLIFSKKIQPQFINPTFIIDYPEEISPLAKSKKDNEEITERFELFIGG
                     LEVANAYSELNDPIEQLKRFQEKAKGDEEKIDFDYIEALEYGMPPAGGLGIGIDRLVM
                     LLTDSPSIREVIFFPLLRPKK"
     assembly_gap    16009..16108
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            16147..17661
                     /gene="murJ"
                     /locus_tag="PLW95_04280"
     CDS             16147..17661
                     /gene="murJ"
                     /locus_tag="PLW95_04280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOV21880.1"
                     /translation="MSSKKMLKAVYIISIGTIISRLFGLFREIVSANFFGTTFIYDAF
                     LIAFMIPNFFRGIIAEGALNAAFIPVFTEYITDENKKKESNKVINLCFTASIIITFAL
                     FFITYILSCFIPNFLSSQSKWFWIWTLLKFTFPYLIFISLTSLNMGILNAHKKFLLPS
                     FSPIILDIFWVTSLFCFLPFFGNSVDERIFGLVIGIILGGIFQFLYLLPSVNKIGYTY
                     KLDFDFSHPAIRKIGRLILPVIVGVAVGPINLLVDYSLAGTLFNGAVSGLWYATRIFQ
                     LPLGVFAISISTASLPYFSENISSKNFIQFKENFYSAMKMLFFLLVPFTFGLILFRSE
                     IITLLFKRGMFGSQSVEIVAFPLMFYSLGLIGYGGASIITRAFYSFSDTSTPVKIGII
                     SILINFVLDITLMKVLSTGGIALATSAIGIVNFFFLYFYFTKKYIKLDNKKIISYFAT
                     SLISSLVMTYVLILYKMKFGNLPLIIFLTTAIILAVIIYFLIWKLLNKILWWNK"
     gene            17646..18248
                     /locus_tag="PLW95_04285"
     CDS             17646..18248
                     /locus_tag="PLW95_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOV21881.1"
                     /translation="MVEQIIILVIFFFSVIIHEISHGYIALLNGDDTAKIMGRLTLNP
                     ISHIDPFGTIIFPLFLFLIHSPFIIGMAKPVPINPFNFRDQDKGMLTVGLAGPLSNIL
                     LGTIFALLVRFLPAGPGRDIFLFSAYINYILAFFNLIPIPPLDGSRVLAVFLPYNIRI
                     RYESIERFGIFIVIIILFLFNIGNLLFTFTQAIVNPIAGL"
     gene            complement(18291..18995)
                     /locus_tag="PLW95_04290"
     CDS             complement(18291..18995)
                     /locus_tag="PLW95_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOV21882.1"
                     /translation="MSNELIVLMTTAATIGFVHTILGPDHYLPFIALSKARKWTKIKT
                     TMVTLLCGIGHVLSSVVLGFIGIALGIAVFKLETIESLRGELAAWFLIIFGFTYFIWG
                     IHKAIYSQSHEHFNKCENGHIHSNKQIGEHINIHSSKSDNLTPWVLFIIFIFGPCEPL
                     IPLVMYPAAKGNMINVAITTFIFGLTTISTMLCIVLSLSYGLSMFPLRKFEKYSNALA
                     GLIIFLCGGAIKFLGL"
     gene            19171..19476
                     /locus_tag="PLW95_04295"
     CDS             19171..19476
                     /locus_tag="PLW95_04295"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HOV21883.1"
                     /translation="MKDNLFEFVISFFATWGWQALGKIPDPVSGKTQKNIEMANNIIE
                     ILTMLEVKTKGNLIEEEARILRSTITDLQLNYVDELKKEQKKEENGKTDKEQTEKNP"
     gene            19436..20893
                     /locus_tag="PLW95_04300"
     CDS             19436..20893
                     /locus_tag="PLW95_04300"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOV21884.1"
                     /translation="MEKQIKNKLKKIPDCPGVYIMKNKNGEIIYVGKAVCLSKRIKSY
                     FNSSGNNTKVLVLSEQINDFEVIPVASEAEALILENQLIKKYQPKYNINLKDGKSYPL
                     LKITNEKFPSVYMVREKRDNNSTYLGPFTNTKLLKDTVMFIRKFYPLRNCKKNIYSGK
                     TKLCTQFYIGLCSGPCENKISKEEYEKILEGFISFFKGEYKKFEKKLKMWMDEAIKKL
                     DFEQAEKLKQRIFLLEKIKEKFPLRDEQALLSYGDENTHFSLSKILGLKKIPYCIEGY
                     DISNISGQLAVGSKVSFKGGIPDRSEYRKFKIKMVEGIDDYKMLQEIIERRFNSEEER
                     KHLPDLILIDGGKGQLNMVAEKLKEIKIDVPVISIAKKFEEIYTLNKNHPIKLPDDSP
                     ELNLLKEIRNEAHRFAVSYHRKLRNRNFKASFLDEISGVGEKTKKKLIKHFQNLSELK
                     NTKIETLKKLGIREKVATEILKKYQSMEVNSPQGR"
     gene            20900..21139
                     /locus_tag="PLW95_04305"
     CDS             20900..21139
                     /locus_tag="PLW95_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21885.1"
                     /translation="MKEEKSLFEEILDIGYGSMRYFSKKAKKVFEEIKEEGKHKQPSN
                     IGKVCELVKETPKIFAKRFIKNLGFVTKEDVKKES"
     gene            21144..22130
                     /gene="obgE"
                     /locus_tag="PLW95_04310"
     CDS             21144..22130
                     /gene="obgE"
                     /locus_tag="PLW95_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOV21886.1"
                     /translation="MKNFIDEVKIKVKGGNGGNGCIAFRREKHVPKGGPSGGDGGKGG
                     NIIFVGNRNVRTLIDFYYQNFLKGENGEHGSGNNKKGKDGVDIKVNVPCGTVIKEINE
                     DKEKIIGEVLKDGEEIIVAKGGEGGKGNTNFKSSTNQAPRIATKGEEGEEKYLKLELK
                     LIADVGIIGYPNAGKSTFISKISNAKVKIANYPFTTLIPNLGVVQLPDFRSFVVADIP
                     GIIEGASGGKGLGIRFLKHIERTKILVHLIDVSEPDPVSRYYKLREELEKYSKKLLEK
                     EEIIVGNKIDIEGTEENIKILKDTFKDVYFISALTGKGINNLLEVIWNKLKG"
     gene            22132..23250
                     /gene="prfB"
                     /locus_tag="PLW95_04315"
     CDS             22132..23250
                     /gene="prfB"
                     /locus_tag="PLW95_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012674642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOV21887.1"
                     /translation="MNTELIQLKEHFEKLKGIWEELKLTYDFKKKQDEFINEDKEREK
                     KGFWNPKEDKEKIEKWTKLNSEIEKIKEINNMFSEVETILELLKNNPDEELENECEKI
                     LSNLEKEISLLKIKFMWKDEADKKNAIITLHAGAGGTEACDWVSMLWRMYNKWASKNG
                     FEVQVVDFLPGEEAGIKRITGIISGPYAYGYLKGENGVHRLVRVSPFDANKRRHTSFA
                     AVNVIPEVENDMKIEINPEDIEIETFRAGGHGGQNVNKVETAVRIKHKPTGTVVQCQN
                     ERSQFKNREIAMRILKSRLYQLEIEKQQKEEIEKRNLQKKIEWGSQIRSYVFFPYTMV
                     KDHRTGYETSNVESVMDGEIDEFLEKELEWLIKNESSQ"
     gene            23580..24218
                     /locus_tag="PLW95_04320"
     CDS             23580..24218
                     /locus_tag="PLW95_04320"
                     /inference="COORDINATES: protein motif:HMM:NF016051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HOV21888.1"
                     /translation="MKIVITAGPTREFIDPVRFISNPSTGKIGYLIAEKLKSKGHSVT
                     LISGPSCLKPPEKVKIVNVISAEDMKREVLGYFPECDILIMSSAVGDWKPEIKSNKKL
                     KRKKEWYLKLIPNPDILKEVSKIKKKNQIIIGFALETENIKENALKKLKGKKLDLIIA
                     DTPGFFGDSISSKVIFISKDEKIEEFEKISKEEVAEKIVKWINFFLSQGKYE"
     gene            24211..25236
                     /locus_tag="PLW95_04325"
     CDS             24211..25236
                     /locus_tag="PLW95_04325"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOV21889.1"
                     /translation="MNNRERFKLTMMFKPVDRLPMIEWATWWDKTLERWYSEGLPSIL
                     IDDGEIREYFGLDCYRQIWIRAIGDNCPAPKTYGAGLIKDIKEYKEIKKYLYPKNFFD
                     NIFPILEKWSEKQKKGDMVIWITLDGFFWFPRQLFGIEQHLFAFYDNEELMHQINSDL
                     VEFHLWAINEFCKICTPDFMTFAEDMSYNNGPMLSKKCFDKFLAPYYHKIIPSLKKKG
                     IIPFIDSDGNIFQMIKWIERVGIEGGLPLERRAGTDIIKIREFFPKFKMIGGFDKTVM
                     SQGEKMMRQEFERIFPIMSQGGYIPSVDHQTPPDVSLKQYHCYVSLLKEYCEKAAKVY
                     SDIYLHK"
     gene            25250..25918
                     /locus_tag="PLW95_04330"
     CDS             25250..25918
                     /locus_tag="PLW95_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOV21890.1"
                     /translation="MNQKFSKLYGFTLIELLVVIAIIAILAGILLPALSQAREKARQS
                     TCINNLKQIYFALEMYGNDYNEFYPLCNGTCLWGTGNQDPPNLGYPGWCEQLYPYIKN
                     KTIYRCPSYPLRQEQFTYFLSAKAAFVDAENKREAVNRKKIRYPSAFILVADNTYSNF
                     TLNDCDKDDYSQNCLVWAKNINHFGAYHLSGLNILFADGHVNWNTNYQQNKITFRYYE
                     YSDW"
     gene            complement(25931..26809)
                     /locus_tag="PLW95_04335"
     CDS             complement(25931..26809)
                     /locus_tag="PLW95_04335"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOV21891.1"
                     /translation="MREILSAIISVITWGTVFVFGQITVKEGCHPILLSFLRFFFASI
                     FLFIYLSLNRIKFKINKDDIPSFIILGLTGIFGMSIFIFYSLKFTTSISSSILMNSNS
                     IFIGILAYMFLREKVRINEIVGIILGFIGCYFVITDGKFSFDMSFIGNLLALFSAISW
                     AFYTVWSKKSNVIKKYGALLSTFWASIFGTFFLFLALLILKIPFSPGKIGIISGIYLG
                     IIPTGIGFTLWFYAINKIKTIVAGILHFISPLTTGIIAVIWFGERISIFTYLGVVLIV
                     FGVVISISRSEKEISN"
BASE COUNT        10484 a         3344 c         4503 g         8386 t
ORIGIN      
        1 tattattgag ttccatttat atttcctgta taataataaa aaaggtgaaa aaatggtaaa
       61 aacaattgaa gaaaaatgtc ggctcttgaa ttatcaatta tatttacttc atactatttc
      121 aaaaacgata aaagaaacat ttaatgaaaa caggatactt aatgtaattc ttacaggact
      181 tacagcaaat ggagcaattg gtttaagtag agcagcagtt ttttactatg atagagataa
      241 aaatatcata tatgggagaa aaggaatagg cccttttgat aatgaggaag catataaaat
      301 atggagtgaa ttaagtaaaa aaaatatctc ccttgaagaa tatctttcat ataatcttga
      361 aaaagaactt gcaacacaaa aattcccaca aacaattaga aaaatagtaa ttaatttaga
      421 tgaactaaat gaagaaaatt attttaaaag agtaataaaa gaaaaacaaa tttttcatat
      481 gtatatggat aacaagcagg caagtattta tttaccaaaa gaaatagaaa caatatttat
      541 accttctgag atagtattac tacctctata ttcttcaaaa gatattatag gtatcattat
      601 ggctgataat gcttttcact ttcaccccat tgatgaatcc acattattac ttatttcgtt
      661 aatcagtata caaacaggaa ttgcattaga aaatgccaat aattacaata ttgttcaaaa
      721 acaacttgaa gaattaaaag aacttcatag tgcaatggaa agtatgcagg aagaattagt
      781 taagaaagaa aaactatcaa ccatagggaa aatggcatct tattttattc atgaaattaa
      841 aaacccactt gctactgctg gtggttttgt aaaaagaata ttggaatcaa atgatatttc
      901 ctctattcat agagatgcgg atattgtttt gaaagaaata aaaaaacttg agcaaatttt
      961 gaataaattt tcaaatttta ctttacttac tccaatgaaa atagaaaaaa tttctttact
     1021 ggatatagtt aaagaaattc ttgacacttt tgaacttgaa ttatctaaaa aatatataga
     1081 tgtgcaaatt gatatacagc aaaatatttc attaaaagcg gaaaaggtac aattatatga
     1141 gatattattt aatttaatat caaatgctat tgaaaatatg gaaagaggaa caataaaaat
     1201 atcagcagaa gttgaaaact cacttttgaa aatatctgtt gaagatactg gaaaaggcat
     1261 accgaaagaa aatatccctt atgtaataga gccgtttttt tctacaaaaa atgaaggact
     1321 tggacttgga ctttttattg tgtcaaatct tgttgaaagt tatgggggga aattagaaat
     1381 agaatctgtt gaaaaaaaag gaacaaaagt aaaaatatat ttccctataa taaattaggg
     1441 aaaggggggg aaatgataaa gaaaaaaata cttattgtgg aagatgtaat aaatgagcag
     1501 attctttata aagaagagct tgaaaaagaa ggatttgaag tatcctgcgt atcaaatgga
     1561 atagaagcaa tagagtttgt agagaaaaat ttgtgcgacc ttgttatact tgaccttcat
     1621 atgccaaaaa tggatggtat agaaacattg ggaaaattac tgacaaaaaa aagaaatctt
     1681 cctatagtaa tttatactgc ctatcctcaa tatagagaac agtttttaac atgggcagca
     1741 gatgcatact taattaaaaa ttcagatatt gacttattaa aaaataaagt gaaagatatt
     1801 ctaagtgaaa aatgaattta aaagatatta caatatttat tcttgctggt ggaaaaggag
     1861 aaaggttata tcctctcaca agagatagaa caaaacctgc agttcctttt ggtggaatat
     1921 atagaattat tgattttcca ttaagtaatg caataaattc tgggctaaga aaaatttata
     1981 tttttacaca atacaaatcg ctttctttac acagacatat tagagaagga tggaatatat
     2041 tttcaaggga attgggagaa tttattgatt ttgttcctgc ccaacaaaga attggtgacg
     2101 aatggtatca aggaactgct gatactgttt ttcagaatat caatatactt gaattagaaa
     2161 aaccaaaact tatacttata ctttcaggtg accaggtata taaaatggac tatatgagaa
     2221 tgataaatga acatcttaaa aaaaaagcag acatgacatt ggctacattt gaagttccac
     2281 ttaatgaagc acatagattt ggagtactta gcgtagacaa agataatagg ataatagaat
     2341 ttaaagaaaa accaaataat ccctttcctt ctcctgaaaa tcccgaagtt gcacttattt
     2401 caatgggtat atatatattt aatcttgatg ttctaattaa aaaaattata gaagatgcta
     2461 aaaaatcaga aagttctcat gattttggaa aggatattat tccttcaatg gttggtaaag
     2521 ataatattta tgctttcaga tttatagatg aaaataaaaa aaatacaaaa tactggaggg
     2581 atataggaac aattgactca tactgggaag caaatatgga tttagtaaat gttgaccctc
     2641 ttttaaatct ttatgatagg gactggccta ttagaacttt tcaggaacaa tatccgccag
     2701 cgaaaactgt cttttcaact ggagatagaa aagcatctgt cactgataca atgctttcca
     2761 gtggctgtat tgtaagcgga agtaaagtaa ttcatagtgt actttcatca aatgttcggg
     2821 ttcatagtta tgctgaggta attgatagta ttttatttga aaatgtaaat attggaagac
     2881 attgtaaaat aaaaagaacc attatagata aaaatgtgga tattcctcca catactgaaa
     2941 taggttataa tttagaagat gcaaggaaaa gatttcatgt atcagaaaac ggaatagtag
     3001 taatacctaa aaactacaaa tggtgaggtg aatatgtcag aattaaggaa agaccctgta
     3061 ataggaagat gggttgttat agcaacagaa agaaaattca gaccatcaga ttttataagc
     3121 agtgaggcag agacatcaaa agaaacattc tgtccatttt gtcctggtaa tgaagataaa
     3181 acaccccctg agatattttc tataaggcat ggtagtacac ctgccaatac tactggctgg
     3241 gaagtacgag ttgtgccaaa taaatttcct gcattaagga tagaaggaga actgaataga
     3301 aggggggtag ggatgtatga cataatgaat ggtattggtg ctcatgaggt aattattgag
     3361 acaccccaac acaaaaatgc aaataaagaa aattaaactt gttgaggcaa ttataaagtc
     3421 tgatnnnnnn nnnnatgcaa ataaagaaaa tatatatatt aaagattact ctataaaaac
     3481 tatgacaaat ataattgaaa catatcaatc aagatgcatt gatttaaaga aagatatccg
     3541 tttccgttat gttcttgtat ttaaaaattt tggtaaagag gcaggagcat ctttatcaca
     3601 tccacattct caaattatag caactcctat tgttccaaaa aacataaaag aaaaactttt
     3661 tggtgctaaa tggtattttg aatataaaga gagatgtgta tggtgtgata taattaatca
     3721 ggaaatagat agtgggaaaa gagtagttga gcaaaattct gcatttatag taattgcacc
     3781 ttttgcttca agatttcctt ttgagttatc aatattacct ttaaggcact ctcctgattt
     3841 tgaaagtatt gactcaaaag agaaagaatt gcttgcagaa ataatggtga gtactttcaa
     3901 aaaaatttca aaagggcttt ctaatccttc ttacaatttt atgattctta ctgctcctgc
     3961 aagatattcc cgtcctggtt attggcaaac attggaaaag gattaccact ggcatataga
     4021 aataatacca cggattgtaa aacagggcgg atttgaatgg ggaactgggt tttacataaa
     4081 tccaacatca ccagaagaag taaccgactt tcttaaatca cttaatgtat aggagggata
     4141 tatggagaaa aaaaatattt gtttagttgc agcagaaggt gtaccatttg tcaaagtagg
     4201 tggaatggca gatgtagtcg gcgctttata taagtatctt aaatcaaaac ataacttata
     4261 tctttttcta ccattttata aaaaaataaa agaaagttat aaaacagaaa aaattggtac
     4321 ctatgatgta tttttttcaa acgatagaca ggagaaaggt tttgtcaata aaagtattga
     4381 tttccccggt atttatttca ttggtaatga caactatttt gaaagagatg atgtttatgg
     4441 accagatggt aaggactatc ctgacagtgc tgaaaggttc tccttttttt caaaaagtgt
     4501 tcttgaagtt atgaaaaaat ttaagataaa tgttcatatt tttcactgtc acgactggca
     4561 tactgcactt attccattat atttaaaact atattataaa gatatcttcc ccattgcaaa
     4621 aacacttttt acaatacata atttaggtta tcaaggggtt tttcctgttg ataaatttca
     4681 cattttgggg ttgccatggg aatatttcaa tatggatgaa ttagaatttt atggaaatgt
     4741 caattttatg aaaagtggga taatacattc agaaaaaata aatacagtaa gtccaaaata
     4801 tgcaaaagaa atactcacac cggaatttgg ttataacttg gatggattgc ttagaaaata
     4861 tcaaaacaaa cttacaggaa tacttaatgg tattgactat caaatatgga atcctgcaat
     4921 tgatggaaat atctataaaa aatatagagg ttataaaaat aaattagaaa ataagttagc
     4981 cctacagaaa agattaaaaa ttcagcaagg aaaagaaata cctgtttttg gaatgatttc
     5041 acggcttgta gaacagaaag gaattgacca attattagaa ataatggaaa gtatcctttc
     5101 tttaccatta gaaattgtta ttttaggaga cggagaacaa aaatataaag aagcattatc
     5161 taaatggcag caagatttcc ctaaaaaact atctattaca tttgcatttg atgataaact
     5221 tgcacatcaa atatatgcag ggagcgattt tttccttatg ccttctaaat ttgaaccatg
     5281 tggattaggg cagatgataa gtttcaaata tggaacagtt ccaatagtta gaaaagtcgg
     5341 aggacttgct gatactgtta gtaattataa ttttgaaaca gaagaaggaa ctggttttac
     5401 ttttgagggt ggggcaaaag aattcttttt atgcatagaa aatgctttga aagtttttgc
     5461 agataaaaat aaaatggaac gacttgtaaa taaaattatg aaactcaatt tttcatggaa
     5521 aacatcaata gaaaaatatt tgaaaattta tgaggaattg atggaacaat gatatatctt
     5581 tcaatcctct ggcatatgca tcaaccaagt tatgtattca atggagaaat aaacactatt
     5641 atacctgttt ttcgcacatt atttaattat tatcctatgg cagttcttac agaaaaatat
     5701 ccagatataa aaattaattt taattttaca cctgtgttac ttgaccaaat tcagggtata
     5761 acttctgcaa aatattatga caattttctt tctatccttg tagatgatac aaatgatgaa
     5821 gaaaaaattt taacttttgc ttctgagata cctggaagaa ttcttgaaaa acaatatacg
     5881 cttaaaatcc tattagataa agttaaaaca aacacttttt ctgttcagga tatttctgat
     5941 ataaaagttc atcttcattt agcatgtttt catccattta ttattgatga agaacttaaa
     6001 caactggtta aaaaaagtag atattttgat aaaaaagaaa aggaattatt atacaacaag
     6061 gaaaaaaata tattgaaaga aacaattaac aaatataaaa atcttatgaa aacaggacag
     6121 tgtgaaataa taaccagtcc tatgacacat ccaataatgc ctttacttta taataccaat
     6181 atagcaaaac aaacaaaaac atcattacca attcctgaaa atcttttttc atatccagag
     6241 gatgctgaag aacaattaat tagaggaata aatatattta aaacaacttt tgaaaaatgc
     6301 cctgatggta tatggccacc agaaggaagt ttaagcaatg aaattttgga tttatttgca
     6361 aaacacaata taaaatggac agcaacagac cagcagttat tatcagaaac actttcaaga
     6421 cctctttttc aaaaagaaca ttataatatt tgggatttta gagaaaaaat tttcattttt
     6481 ttcagagacc atcatctttc tgatatgata ggttttagtt atcaacatat ggaagaaaaa
     6541 gaagcagcag ttaatttcat tgaacatatt cataaatcgt cagataaaac aaaaaatcaa
     6601 ttaattacaa ttatacttga tggtgaaaat ccatgggact attacccttc tttcggtttt
     6661 tactttcttc ctgctttata cacaatgtta agtgagtcat ctgatataaa aactattaca
     6721 ttttctgaag cactaaaact tgatataaag catgagaaat tagaaagaat agtgcctggt
     6781 agttggatgg gagtaaattt tgacaactgg ataggcaaac aaccagcaaa tactgcatgg
     6841 aaaatattac aacaagttag aaaactttca gaagaaaaaa ttaattcctt agatgataca
     6901 aaaaaagaaa aattgaaaga atatattatg cttgcagaaa gtagtgattg gttctggtgg
     6961 tatagtatac cagcagataa aaaaattaaa aggaaatttg atgcatattt tagaaataat
     7021 ataagaaaaa tttatgaaat tcttgatgta aaggcacctg aatttttgaa ttttcctgtt
     7081 gagaactatg atattgaaga attttttcca tatattaaac catctattga tggtaaaata
     7141 actcactttt atgaatggta taatgctgtg gaaattgatg ctgctgattt atggacaaca
     7201 tttaagccag ttgaaattcc aataaaaaaa atattttatg gttatgatga tgaaaatgtg
     7261 tatataagaa tagacactct tgaaaaacaa aacattgaaa ttgaaattac ttttcataat
     7321 tcagtaaaac aaaaattcta tataaaaaat gacctatcca gttctttacc aatatcattt
     7381 gtctatggcg atataataga aataaaaata cctaaaaaag agatattact tgaaggagaa
     7441 aagacaatat tttttaatat ttctataaga gacagagaag gacatgaaat tgttttgcct
     7501 actggcgatt attttaaaat aactttttta tccgaggagg agaattggat aatatagaaa
     7561 aaaatcaacc gcctaacaga atatctcttt ttgggccctt ggacctttat ttatttaacg
     7621 aaggaaatca ttttgaaata tataaaaaat taggtgcaca taaaagggta gtaaatggaa
     7681 tagagggcta taactttgct gtttgggctc caaatgctgt aaaagtaaat gtaaaaggca
     7741 atttcaataa ttgggatgga agtaaacatc aaatggttga gttaggtaat tcaggtatat
     7801 gggaaatatt tattccagaa gtaaaagagt gctacccata caaatttgag attcatacca
     7861 aaaataataa tgttcttctt aaatcagacc catatgggtc tttttttgaa cttcgtccag
     7921 ccaatgcatc tcttacatat aaatcacatt ataaatttga aaattctaaa atccaaccta
     7981 ccaatatata taaacaacca atggctatat atgaaatgca tcttggttca tggaaaagaa
     8041 gaaaagatgg atttttttta aactatcgtg aaattgcaga agcccttgtt ccttacctga
     8101 aagaaacggg atttaattgg gttgaattca tgcctatcag tgaacatcca ttagattctt
     8161 cctggggtta tcaaacaaca ggattttttg ctcctacaag tagatatgga acacctgatg
     8221 atttgtgttt tcttatagac caactgcata aaaataatat tggtattatt cttgactgga
     8281 cacctgctca cttcccaaaa gacgattttg gtttatacct ttttgatgga acacatttgt
     8341 atgaacacta ctttcctcag aaaagagacc atcctgattg gaaaacagcg atatttaatt
     8401 atggaagata tgaagtaagt aattttctta taaatagtgc tttaaactgg attgaaaatt
     8461 ataaaataga cggacttaga attgatgcag ttgcatcaat gctttacctt gactattcaa
     8521 ggaaaaatgg ggagtggatt ccaaataaat atggtgggaa agagaactta gaagcaattg
     8581 attttttaaa aaaactaaat agtactattt atcaaaaaca cccaaatact tttacaatag
     8641 cagaagaatc aactgcctgg ccaatggtaa gtcgtcctgt ccatatgggt ggattgggat
     8701 ttggttttaa gtggaatatg ggatggatgc atgatattct ctactatttc tctcttgacc
     8761 caatatatcg taaataccat cataactgtt taacatttgg acttttatat gccttttcag
     8821 aaaattttgt tttaccaatt tctcatgatg aagttgttta tggaaaaaaa tcacttctct
     8881 caaaaatgcc aggagacaca tggcaaaaat ttgctaattt aagaacactt ttaacatata
     8941 tgtactgcca cccaggtaaa aaacttcttt ttatgggaag tgaattcgga caattatcag
     9001 aatggaacta taatggagaa cttgaatggt ggttacttga aaaagaagaa cacagaagca
     9061 taaaaaaatt tgtatcagac ctcaatactt tatataaaaa tgaaaaagca ctttttgaaa
     9121 ttgattttga aaataatgga tttgaatgga ttgatttctc cgattatgaa aagagtatca
     9181 tatcatttat tcgtaaaggt gaaaaagaaa ataattttat aattactgtt ttcaatttta
     9241 cacctgttcc ccgatataat tacagaatag gagtcccttt aaaaattcat tataaagagg
     9301 tatttaatag cgattcaaaa ttttatggag gaagcaatac agggaacttt ggtatggtag
     9361 aaagtgaaaa tataccatat cataacagac ctttttctgt aaatctcaca atacccccac
     9421 tatcaggtat cattttgaaa ccacatattg acagttttta aaaatatgat aaattataaa
     9481 taagattgga aaagaaaaaa gggggaaaaa tgaagaaaat aattatctgt gtttttctgg
     9541 ttagttcttt aactttgttt ggagaagtaa aaacagaggt ctctacatca ggaccttctg
     9601 tttctgaagt tctacaatac cagggaccta aagcaagaat ttcagttgga aaatttgaag
     9661 taaaggcagc aaaaggtgca tgggaaattg gagatggact taaagagatg ctggttgacg
     9721 ctttatttaa aacaggaaga tttattgtgt tagaatcatc tgaaaatatt gatacattaa
     9781 aagaagaatt tgaattagga gaaagtggat ggagtaataa agcaccagaa aaaggaactt
     9841 ttgaaacagc agatataatt ttaactggtg ccataactgc ttttgaacct gattataaag
     9901 ggaaaggtgg aggcggagtg gttgtacctt taccgtttgc tgttggaggt ggattaaaaa
     9961 ttagcaaaca agaatcatat attgcttcta atttacgact tgttgatgtt agaaccagaa
    10021 gaattataaa aacagcaaaa gtagaaggat ttgcatcaaa aagtgatata ggcgttttag
    10081 gaggtggttt aattggttct gttgctcttg gtgctggctt tcaaaaatat aaaaatactc
    10141 caatggaaca agcagttatg attatgttag aaaatgctgt taatgagatc attaaaagtg
    10201 tcccggaaga ttattacaga tatgatgaaa aaggaaatgc tattactgca aatcaacaaa
    10261 cagcaactgc aagtcaacaa acaacagaag aaacaaaaat aaaaattgtt gggggtggag
    10321 atatatttaa ggcaggtgaa gctattattt taaatgagga ctttaataaa tatgaaatag
    10381 gagatatccc aactgatttt caatatgaaa aggcagaagt agaaattgca gaaatgaatg
    10441 agaaaaaatg gatgaggttt ctaaaacagg gaaatataaa aaaagacata aaagtagaag
    10501 gagattattc ttatgagatt tcttttctca caacatttaa ttgttcaact gatattgtct
    10561 taccggatgg caaatcaatc catctaacta atgggaaaat tctatattct gaaaatgagt
    10621 tatctcctat aagtattaat aaagtcaata aaattcaatt atcaaaaaaa gagggtataa
    10681 caagaatatt tgtaaatgat aaaagggtat ataaagcacc tgaaaaactg gatacattat
    10741 caaatggtat tgttattaaa tgtgataatg tttctcctga tgacggtaaa gaattccttt
    10801 tcacagacct taaaattgca acttataaat gataataggg attgattgtg gaaattctta
    10861 tataaaaatt ggtttattta aagaaaaaat aataaaacag gtagttaaat ttcctgttga
    10921 agatataaat aaatttagaa ttccttacca ttggaaaaaa ctaaatattg aagttattgc
    10981 attagcaagt gtctcaccta tcaattctga taaaataaaa actaccttct cttccttttt
    11041 caataaaaaa gtaattacaa taacttctca acaatgtaat atccccctta atataaaaga
    11101 caaaaaaaaa gtcggggttg atagagtcct aaattgtaaa ggtgcctctg ttctttatgg
    11161 aaacccatca atagttatag acataggcac tgctattaca attgattttg ttaatagaaa
    11221 tggtgttttt gaaggaggaa taattttccc tggtccattt ttattactca attcactcaa
    11281 taaaacatct ttgataaaat caccaaattt tttaaaaaag aacattttaa ttgggaaaaa
    11341 tactgatgaa tgtataacat ctggtttaaa taatggaata gttggttgta tagaaaatat
    11401 agtgggaaaa ataaaaaaca tacataaaaa ttcaaatgtg attttaactg gtggctggtg
    11461 tgattatttt gctaaaaaaa ttaaaattaa aaaggttgtt aaaaaaaatt taacattaga
    11521 aggaataaac attgtaatta gagaaagatg gttgaagaaa taattgccgg tgaaaaagtt
    11581 tatttttatg atgaggtaag ttcaacaatg gatgtagcaa aaaaactcct taaagaaggt
    11641 gaaaaaggaa ttgttgttgc aaaaaaacaa acgaaaggaa ggggaagata tgggaaatac
    11701 tggctttctg atgaaggagg actttatttc agtatgataa ttgaaaaaag cgttatatct
    11761 gattttttgt cagaaattat ttcaatatct cttgttgaaa caatgcaaaa atttggaata
    11821 atttgtaaaa taaagttccc aaatgatata atatataaag ggaaaaaaat atctggaatt
    11881 ttaatagaga gaagcgatga taattacatt gttgggatag gagtaaacat aaataataag
    11941 ataagtgagt ttggtatttc aatgaaagaa atattaaaaa aagaaataaa tataaatgag
    12001 tttcttgaaa aatttttaac tatttataaa acaaacgaaa taaaattttg cgaaaataag
    12061 gagaattgtc ttaaagaatg gagtgaattt ttattaaaat gagccaacca gatatatttc
    12121 aaaatttaat ttttattttc atattggtct gtttatctgc ttttttttct gcttcagaag
    12181 ccgcttttat atctctcaat aaatatcata ttcagaaatt ttcagatatt aacaaagaaa
    12241 aagcatctcc aatagaattc tggataaaat acccggaaaa aattttaatt acaattcttg
    12301 ttggaaacaa tattgtaaat atacttgttt ctataattgc tgctattact tctgaaaaat
    12361 tatttcatag aacaagtatt gctttaatta caggtattat gatttttatt attcttgtgt
    12421 ttggtgaaat tattccaaaa tccattggta aaaggcattg tgaaaaattt gttagaattt
    12481 ctttttaccc aattaaattt ctttctatat tacttaatcc tgttataaaa tttcttatat
    12541 ttgttgcaaa tttttttgtt ttaccttttg gacagagaat aaatacaata atgcctgtta
    12601 taacaaaaga agatgttaaa gcaatgattg aagcaggaga agaagaaggt gttattgaag
    12661 aagaagaaaa agaaatgatt catagtatat ttgaaattgg tgataaaatg gttaaagaaa
    12721 ttatgatacc gagggtaaaa attgttggag tagaagaaaa tactaaaatt acagatgtaa
    12781 taaaagttat agaaaaagaa ggatattcac ggattcctgt ttataaaaat aatatagata
    12841 aaattgtggg aattgtaata ataaaagata ttgttgcaca attaaataaa gaaggttcaa
    12901 ctgaactaat ggcaaaagat ataatgagag atgttttttt tgttcctaaa acaaaaaggg
    12961 ttttagaact tttaagagat ttacaaaata agaaattaca aatggcgata gttgttgatg
    13021 aatatggtgg aacttctggg cttattacga tggaagacct tgttgaagaa atagtaggag
    13081 aaatttctga tgaatacaaa aaagacacaa ggccttatca taaattacct gatggaagtt
    13141 ttcttgttca gggaagtatg gagattgaaa aatttgagga gatttttgat tttaaaaaag
    13201 atgaagattc agatgttgaa acaattgctg gttttatact tgattattta ggaaaattcc
    13261 caaaaacagg tgaaaaattt acatataata attttgagtt cacaattcaa gaaagtgatg
    13321 agaaaaatat taaatggata aaagttaaaa aaatagtata atttcagaga agtttattag
    13381 gtcaggtaca aaggtataga ggtacaaata aatgtcccct caccccttcc ctctccccgt
    13441 tggggagagg gaaaaaatag aagagaaaaa atcccttctc ccgtggggga gaaggttggg
    13501 atgaggggag aacctctatt taatccgcca gaaagtgtat cggatgctga aaaacgcact
    13561 ctgtgcgtca gtatagataa tattaaaaaa atggaaggag aagaaaatgg cacaggacga
    13621 gataagagac cagagaataa agaaaattga aattttaaaa agtagaaata ttgaaccgta
    13681 tggaagaaga tacgaaaaaa caaaaacaga agatatagga gaacaggggg aagtaaaaac
    13741 agcaggaaga attattgcat taagaaaaca cgggggagca tgttttgctg atattataga
    13801 tacagaaggg aaaatacaac tatattttaa aaaaaatata attggtgaag aactttatga
    13861 gatatttaaa gaaatagata ttggagatat tattggagtt gaaggagaaa tttttaaaac
    13921 aaaaacaggt gaaaaaacaa tttttgtaaa aaattttaaa gttctttcaa aatcacttaa
    13981 ccccctccct gaaaaatggc atggattaaa agatattgaa ataagattta gaaaaaggta
    14041 tttagacctt ttaattaaca aagaagtaag agaaaaattt ataaaaagaa cgaaaataat
    14101 tcagtttata agggaatatc ttaacaatat ggactttatt gaggttgaaa caccaatgat
    14161 gcatccaata ccagggggag cagaagcaaa accatttatt acatatcata atacacttga
    14221 gatgaatctt tatttgagaa ttgctcctga actttatttg aaacggcttc ttgttggaaa
    14281 ttttgaaaaa atatatgaaa taaacaggtc atttagaaat gaaggaatat ctacgcttca
    14341 caatccagaa tttacaatgc ttgaattgta ttctgcttat ggtgattatg aagaaatgat
    14401 ggaaataaca gaaaaacttc tcacaaatct tgtaaatttt ctttataaaa gttctactat
    14461 tacatatcag gataaagaaa taaatttttc tctaccatgg gaaaaaatta cttatgatga
    14521 tgtttttaaa aaagttggaa ttgaaaattg gcatgatata aattcagtaa aagaaaaaat
    14581 aaaggaattt aatcttgaag tagaagaaga aaaagaaaat aatctttttg aattattaga
    14641 ccttattttt agtaaaaaaa tacagccaca atttataaat ccaactttca taattgacta
    14701 tccagaagaa atttcgccat tagcaaaatc aaaaaaagat aatgaagaaa taaccgaaag
    14761 atttgaactt tttattggtg gacttgaagt tgcaaatgct tattcagaat taaatgaccc
    14821 gattgaacaa cttaaaagat ttcaagaaaa agcaaaaggt gacgaagaaa aaattgattt
    14881 tgattatatt gaagcacttg aatatggtat gcctcctgct ggtggacttg gtattggaat
    14941 agaccgcctt gtaatgcttc tgacagatag tccttcaata agagaggtta tctttttccc
    15001 ccttctacga cccaaaaaat gagttcaaac cacagcaaaa attatgagtt aagcacagca
    15061 gagacacaaa gttaaaattg gaagatagaa gaacattgtt ctctggaaga atcggtatcc
    15121 ttgaaaggat aaggtaataa gttccccctt tagccaaaga gggtaggggg atttaataaa
    15181 atcccgtaag cataacttat cgcttcggcg ctttcccgtc tgagcattag cgaagaaggg
    15241 taaggatggt ctgtaagcaa aactctaaac tcttctgtgt aagaccctcc attcccccct
    15301 ttaataaagt ggttcttccc ttttgcaaag ggaagttaga agggatttag ggaagtaaga
    15361 gaagattttt taagagggag tgagagaagg ttggggtaga aagtgaagaa tcccggccaa
    15421 atgtctggac ttctttaaaa gcaccataac ctcagttgtt catatgaact actgtgcttt
    15481 tctatatatt gcctcactac cttgtgcaaa tttgacagca aatttattgt tttgctttaa
    15541 ttcccccctc accgaacctc tccccagcgg ggagagggca gggtgagggg ggatttgatt
    15601 tctcccttta taaaaccgat taaagtagcg aaggttagca gggcagagtt ggtgcttgtt
    15661 tcccctcttt attaaagagg ggttagggga gattttccaa gcacaacggg aatagaggga
    15721 ttttcaaaca caacggagat tagaggggaa tttagatatt tttctttttt gtaaagtata
    15781 tcctacatga tttgtggtgg aaggggtaag gggagatttt tgtttccctt ttattttctt
    15841 ctccccttgg ggagaagttt agatgagggg gaatttttta tttaatatcc gtcaaaaacc
    15901 atagcggacg ctaaaaaaca cgctttgtgc ttctgtgcct ttgtgtcttt gtgcctaaaa
    15961 tatacacaaa tttaacttct attaaaaaaa aatgagttca aaaaaaatnn nnnnnnnnnn
    16021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16081 nnnnnnnnnn nnnnnnnnnn nnnnnnnncc taaaatatac acaaatttaa cttctattaa
    16141 aaaaaaatga gttcaaaaaa aatgcttaaa gcagtttata taatttcaat tgggactata
    16201 atttctcgac tttttgggct ttttagagaa attgttagtg ctaatttttt tggaactact
    16261 tttatatatg atgcttttct tattgctttt atgataccca atttttttag aggaataatt
    16321 gctgaaggtg ctttaaatgc tgcatttatt cctgttttca ctgaatatat aactgacgaa
    16381 aacaaaaaaa aagaaagtaa taaagtcata aatctttgtt ttactgcttc aataattatt
    16441 acatttgctc ttttttttat tacttatatt ttaagttgtt ttatcccaaa ttttttatcc
    16501 tctcaatcaa aatggttctg gatatggaca cttcttaaat ttactttccc ataccttatt
    16561 tttatttctc ttacttcttt aaatatggga atattaaatg ctcacaaaaa atttttatta
    16621 ccttcttttt ctcctataat tcttgatata ttctgggtta cttctctttt ctgcttttta
    16681 ccatttttcg gaaattcagt tgatgaaaga atttttggac ttgttatagg aataatatta
    16741 ggagggattt ttcaattttt atatctctta ccatcagtca ataaaattgg atatacttac
    16801 aaacttgact ttgacttttc acatcctgct ataagaaaaa ttggaagatt aattttacct
    16861 gttattgttg gtgttgcagt gggacctata aatttacttg ttgattattc acttgctggg
    16921 acattattta atggtgctgt ttctggatta tggtatgcaa caagaatatt tcaacttcca
    16981 ctcggtgttt ttgctatttc aatttcaact gcatcacttc cctatttttc agaaaacata
    17041 tcttcaaaaa atttcataca atttaaagaa aatttctatt ctgcaatgaa aatgctattt
    17101 ttcttacttg ttccatttac atttggactt atattattta gaagcgaaat aattactctt
    17161 ctattcaaaa gagggatgtt tggaagtcaa tcagtggaaa ttgttgcttt tcctcttatg
    17221 ttctattcat tgggacttat tggttatgga ggtgcttcaa ttataacccg tgctttttat
    17281 tctttttcag atacatcaac accagtcaaa attggaatta taagcatatt gataaatttt
    17341 gttcttgata ttacacttat gaaagtttta tccactggtg gaattgctct tgcaacttca
    17401 gcaattggca tagttaattt ctttttttta tatttctatt ttacaaaaaa atacataaaa
    17461 ttagacaaca aaaaaataat ttcttatttt gcaacatctc ttatatcttc actggtaatg
    17521 acctatgtac ttattcttta taaaatgaaa ttcggaaatt tacccttgat aatatttctg
    17581 acaactgcta taattttagc agttataatt tattttttaa tctggaaact tcttaataaa
    17641 atattatggt ggaacaaata attattctgg ttatattctt tttttctgtg ataattcatg
    17701 aaatttctca tggatatatt gctcttttaa atggagatga tactgcaaaa ataatgggta
    17761 gattaacatt aaatcccatt tcacacattg acccttttgg aacaattatt tttccacttt
    17821 ttctttttct tattcattct ccatttataa ttggtatggc aaaaccagtt ccaataaacc
    17881 ctttcaattt tagagaccag gataaaggaa tgttaactgt tggacttgca ggaccattaa
    17941 gtaatatatt attaggaact atttttgctc ttcttgtaag atttttacca gcagggcccg
    18001 gtagagacat atttttgttc tctgcatata taaattatat tcttgctttt ttcaacctca
    18061 ttccaattcc tcctcttgac ggctcccgtg ttcttgctgt ctttctccct tacaatatca
    18121 ggataagata tgaatcaatt gaaagatttg gtatttttat tgtcattata attttatttc
    18181 ttttcaatat tggtaatctc ctttttactt tcacacaagc aatagttaat ccaatcgctg
    18241 gcctctaatt ctaatcctta ttctaacccc cgcggttata cttggttata ttatagtcct
    18301 aaaaatttga tggcgcctcc acaaagaaaa attatcaaac cagcaagtgc attagaatat
    18361 ttttcaaatt tcctgagtgg aaacattgat aatccataag ataaagataa aacaatgcat
    18421 aacatagttg aaatggttgt caatccaaaa ataaatgtag ttattgctac atttatcata
    18481 ttacctttgg ccgcaggata cattacaaga ggaattagtg gctcgcatgg accaaatata
    18541 aatattataa aaagaaccca tggagttaaa ttatcagatt ttgaactatg aatattaatg
    18601 tgttctccaa tctgcttatt ggaatgtata tgtccattct cacacttatt aaaatgttca
    18661 tgagattgag aataaattgc tttatgtatt ccccagatga aatatgtaaa tccaaatata
    18721 ataagaaacc aggcagctaa ttctcctcgc aatgactcaa ttgtttctaa tttaaaaaca
    18781 gcaattccta aggcaatacc tataaatcct aaaacaacag aacttagtac atgtccaatt
    18841 ccacataaaa gtgtaaccat agttgtttta attttagtcc acttccttgc tttagataat
    18901 gcgataaaag gtaaataatg gtctggtcca agaatagtat gaacaaatcc tatagttgct
    18961 gctgttgtca ttaaaacaat aagttcgttt gacataataa tctccttttt gttttaattt
    19021 atcattttct ttcactcgta tcaaatatat gtattacttt ttatgatact tccctttacc
    19081 taatttaatt tcaaggtaat tctggtgttg aaattcggtt tatgaaatta gtataattta
    19141 tatatagaaa taataaaata aggagggcaa atgaaagata atttatttga atttgttatt
    19201 tctttttttg caacatgggg atggcaagca cttggaaaaa ttcctgaccc tgtttcagga
    19261 aaaacacaaa aaaacattga aatggctaat aatataattg aaattcttac aatgttagaa
    19321 gttaaaacaa aaggaaatct tatagaagaa gaggcacgca ttttaagaag tacaataact
    19381 gacttacaac ttaattatgt tgatgaactg aaaaaagaac aaaaaaaaga agaaaatgga
    19441 aaaacagata aagaacaaac tgaaaaaaat ccctgattgt cctggtgttt atataatgaa
    19501 aaacaaaaat ggcgaaatta tttatgttgg aaaagcagta tgtctttcaa aaagaataaa
    19561 atcatatttt aattcatcag gaaataatac caaagtttta gtactttctg aacaaataaa
    19621 tgattttgaa gtaatacctg ttgcttcaga ggcagaagca ttaattttag aaaatcaact
    19681 tataaaaaaa tatcaaccaa aatataatat aaatctcaaa gatgggaaaa gttatcctct
    19741 tttaaaaata acgaatgaaa aatttccatc ggtctatatg gttagagaaa aaagagataa
    19801 taattcaact tatcttggtc catttacaaa cacaaaactt ttaaaagata cagtaatgtt
    19861 tataaggaag ttttatcctt taagaaattg taaaaaaaat atttatagcg gtaaaacaaa
    19921 actctgtaca caattttata ttggattatg ttcaggtccc tgtgaaaata aaatatcaaa
    19981 agaagaatat gaaaaaattc ttgagggatt tatatcattt ttcaaagggg aatataagaa
    20041 atttgagaaa aaattaaaaa tgtggatgga tgaagcaata aaaaaacttg attttgagca
    20101 ggcagaaaaa ttaaaacaac gaattttcct tcttgaaaaa ataaaagaaa aattcccatt
    20161 gagagatgaa caagcacttc tttcctatgg tgatgaaaat actcattttt cactttcaaa
    20221 aattttaggg ttgaaaaaaa ttccatattg tattgaggga tatgatattt caaatatatc
    20281 aggacaactt gctgtcggaa gtaaggtctc ttttaaaggg gggatacccg ataggtctga
    20341 atatagaaaa tttaaaatta aaatggtaga agggattgat gattataaga tgttacagga
    20401 aattattgaa agaagattta attcagaaga agaaagaaaa catttacctg acttaatttt
    20461 aatagatgga gggaaagggc aattaaatat ggtggcagaa aaacttaaag aaataaaaat
    20521 tgatgtacca gtaatttcaa ttgcgaaaaa gtttgaagaa atatatacat taaataaaaa
    20581 tcatccaata aaacttcctg atgatagccc tgaattaaat cttctaaaag agataaggaa
    20641 cgaagcgcat agatttgcag tttcttatca tagaaaattg cgaaatagaa attttaaagc
    20701 atcctttctt gatgaaataa gtggtgtagg tgaaaaaaca aaaaagaaat tgataaaaca
    20761 ttttcaaaat ttatcagaac ttaaaaacac aaaaatagaa acattaaaaa aattaggaat
    20821 aagagaaaaa gttgctactg aaattttaaa aaaataccaa tcaatggagg taaattctcc
    20881 acaggggagg tgataaaaaa tgaaagaaga aaaatctctt tttgaagaaa ttttggacat
    20941 tggatatgga agtatgaggt atttctcaaa aaaggcaaaa aaggtttttg aagaaataaa
    21001 agaagaaggt aaacataaac aaccatctaa tataggaaaa gtttgcgaat tggtgaaaga
    21061 aacaccaaaa atttttgcta aaagatttat taaaaattta ggttttgtta caaaggaaga
    21121 tgttaaaaaa gaaagttaaa taaatgaaga attttattga tgaagtaaaa ataaaagtaa
    21181 aaggtggaaa tggggggaat ggatgtattg cattcagaag agaaaaacat gttccaaaag
    21241 gtggaccatc tggtggtgat ggtggaaaag gtggaaatat tatttttgtt ggaaatagaa
    21301 atgtaagaac tttaattgat ttttactacc aaaatttttt gaaaggtgaa aatggtgaac
    21361 atgggagtgg aaataataaa aagggaaaag atggagtgga cataaaggtc aatgttcctt
    21421 gtggaacagt aataaaagaa ataaacgaag ataaagaaaa aataattggt gaagttttaa
    21481 aggatggaga agaaataata gttgcaaaag gcggggaggg tggaaaagga aatacaaatt
    21541 ttaaatcatc aacaaatcag gcaccaagaa ttgcaacaaa aggtgaagaa ggagaagaaa
    21601 aatatcttaa attagaatta aaacttattg cagatgttgg aataattggt tatccaaatg
    21661 cagggaagtc aacttttatc tcaaaaattt caaatgcaaa agtaaaaatt gcaaactatc
    21721 catttacaac attaattcca aatttaggag tggttcaact tcctgacttc agaagttttg
    21781 tagttgctga tattccagga attattgagg gggcttcagg aggtaagggt ttgggtataa
    21841 ggtttttgaa acatatagaa agaacgaaaa ttttagttca tcttatagat gttagcgaac
    21901 ctgaccctgt cagcagatat tacaaattaa gagaagaatt agaaaaatat agtaaaaaac
    21961 ttttagagaa agaagaaata attgttggaa ataaaattga tattgaagga acagaagaaa
    22021 atattaaaat tttaaaagat acttttaaag atgtttattt tatttccgct ttaactggaa
    22081 aggggataaa taacttgctt gaagttatat ggaataaatt gaaaggataa tatgaatact
    22141 gaactaattc aattaaaaga acattttgaa aaattaaaag gtatctggga agaattaaag
    22201 ttaacttatg atttcaaaaa aaaacaagat gaatttataa atgaagataa agaaagggag
    22261 aaaaaaggat tttggaaccc taaggaggac aaagaaaaaa tagagaaatg gacaaagtta
    22321 aattcagaaa ttgaaaaaat taaagaaatt aataatatgt tttctgaggt tgaaacaatt
    22381 cttgaattac ttaaaaataa tcctgatgaa gaacttgaaa acgaatgtga gaaaatatta
    22441 agtaacttag aaaaagaaat ttctttgtta aaaataaaat ttatgtggaa agatgaggca
    22501 gataaaaaaa atgcaattat aactttacac gcaggagcag gaggaacaga agcatgtgat
    22561 tgggtaagta tgctatggag aatgtataac aaatgggcat caaaaaatgg atttgaagtt
    22621 caggtcgttg atttccttcc aggtgaagaa gcaggaatta aaagaataac tggaataatt
    22681 tcaggaccat atgcttatgg atatttaaaa ggcgaaaatg gagtccatcg gcttgtccgc
    22741 gtttcaccct ttgatgcaaa caaaagaaga catacaagtt ttgcagcagt taatgtgata
    22801 cctgaagtag aaaatgatat gaaaattgaa attaatccag aagatattga aatagaaaca
    22861 tttagagcag gtggacatgg tggacaaaat gtaaataaag ttgaaacagc agttagaatt
    22921 aaacataaac ccacaggaac tgttgttcaa tgtcaaaatg agagaagtca atttaaaaat
    22981 agagaaattg ctatgagaat tttaaaaagc cgactttatc aattagaaat tgaaaaacaa
    23041 caaaaagaag aaatagaaaa gagaaattta cagaagaaaa tagagtgggg aagccagata
    23101 aggtcatatg tttttttccc atatactatg gtaaaagacc ataggactgg atatgaaaca
    23161 tcaaatgttg aaagtgtaat ggatggagaa attgatgagt ttttagaaaa agaattggaa
    23221 tggttaataa aaaacgaaag tagccaatag taagtcaagt aaaaagaaaa aatgaaattt
    23281 aaaatccatc ccaaccttcc tttggtaaag gaaggagtta gaatttctcc attgtataaa
    23341 ccgattaaag aagcgaagat tagcagagat ggtgcttatt tcccctcttt ataaaagagg
    23401 ggcaagggga gatttcaaca agcacaatgg agagaaagag aaattttttt cacagagata
    23461 aataaacaac cagttctata ccaaagtgct aagtagttat aactacagaa tttattttgt
    23521 accttagtac ctctgtgtat tgaatgtgaa taaattcagt aaatagttaa aaagaaaaaa
    23581 tgaaaatagt aattacagca ggaccgacaa gagaatttat agaccctgtt agatttataa
    23641 gtaatccttc aacaggcaaa attgggtatt taatagcaga aaaattaaaa tcaaaaggac
    23701 attctgttac tctcataagt ggtccttcct gtctcaaacc accagagaaa gtaaaaatag
    23761 taaatgtaat ttcagcagaa gatatgaaaa gagaggtttt aggatatttt cctgagtgtg
    23821 acattttaat tatgtcatca gcagtgggtg attggaaacc agaaataaaa tctaataaaa
    23881 aattaaaaag aaaaaaagaa tggtacttaa aacttattcc aaatcctgat attctaaaag
    23941 aggtatcaaa aataaagaaa aaaaatcaaa taataattgg ttttgcactt gaaacagaaa
    24001 atataaaaga aaatgcttta aagaaattaa aagggaaaaa actcgattta ataattgcag
    24061 atactcctgg tttttttggt gatagtattt cttcaaaagt aatttttatt tcaaaggatg
    24121 agaaaattga ggaatttgaa aaaatcagta aagaagaagt tgcagaaaaa atagttaaat
    24181 ggataaattt ttttttatca cagggaaaat atgaataata gagaaagatt taaattaaca
    24241 atgatgttta aaccagtaga ccgcttaccg atgattgaat gggctacttg gtgggataag
    24301 acattagaaa gatggtatag cgaagggtta ccttccattt taatagacga cggtgagata
    24361 agagagtatt ttggattaga ttgttatcgt caaatatgga taagagcaat aggggataat
    24421 tgtcctgccc ctaaaactta tggagcagga ttaataaaag atataaaaga atataaagaa
    24481 ataaaaaagt acctatatcc aaaaaatttt tttgataata tattccctat cttagaaaaa
    24541 tggtcagaaa aacaaaaaaa aggagatatg gttatttgga ttactttaga tgggtttttt
    24601 tggtttccaa gacaactttt tggtatagaa caacatctat ttgcttttta tgacaatgaa
    24661 gaactcatgc atcaaattaa ttctgacctt gttgaattcc atttatgggc tataaatgaa
    24721 ttctgtaaaa tttgtacacc cgattttatg acatttgctg aagatatgtc ctataataat
    24781 ggaccaatgc tttcaaaaaa atgttttgat aaatttcttg ctccatatta tcacaagatt
    24841 attccgtccc taaaaaagaa aggtattatt ccatttattg attcagatgg taatattttt
    24901 caaatgatta aatggataga aagagttgga attgaaggag gattaccttt agaaagaagg
    24961 gctggaacag acataattaa aatcagggaa ttttttccta aatttaaaat gatagggggg
    25021 tttgataaaa cagttatgtc tcaaggtgaa aaaatgatga gacaagagtt tgaacgtatt
    25081 ttccctataa tgagtcaagg aggttatatt ccctcagttg accatcaaac accacctgat
    25141 gtttctttaa aacaatatca ttgttatgta tctttactca aagaatattg cgaaaaagca
    25201 gcaaaagttt attcagatat atatttgcac aaataaaaaa tttaaaacta tgaatcaaaa
    25261 attttcaaaa ttatatggat ttacattgat agaattactt gttgtaatag caataattgc
    25321 tattttagca ggaatacttt tacctgctct ttcgcaagca agggaaaagg caaggcaatc
    25381 aacatgtata aacaacttaa aacaaatata ctttgccctt gaaatgtatg gaaatgatta
    25441 taatgaattt tatcctttat gtaatggaac ttgtctctgg ggaacaggaa atcaggaccc
    25501 accaaattta ggttaccctg gctggtgtga acaattatat ccatatataa aaaacaaaac
    25561 aatatataga tgtccttcat atcctttaag acaagaacaa tttacatatt ttttaagtgc
    25621 aaaagcagca tttgtagatg cagagaataa gagagaagca gttaatagaa aaaaaataag
    25681 gtatccatca gcatttatac ttgttgcaga taacacatat tctaatttta cccttaatga
    25741 ttgtgacaaa gatgattata gtcagaattg ccttgtttgg gctaaaaata taaatcattt
    25801 tggagcatat catctttctg ggttgaatat cctttttgca gatgggcatg ttaactggaa
    25861 tactaattac caacaaaata aaataacttt tagatattat gaatattctg actggtagta
    25921 acaataaatg tcaattagaa atttcttttt cacttcttga tattgaaata acaaccccaa
    25981 aaacaataag gacaacacca agataagtaa aaatagaaat tctttctcca aaccatataa
    26041 ctgcaataat tccggttgtt agtggagaaa taaaatgtaa aattcctgca acaattgttt
    26101 tgattttatt tattgcataa aaccataggg taaaaccaat tccagtagga ataattccaa
    26161 gataaatacc agatataatt cctattttac caggtgaaaa tggaatttta agaatcagca
    26221 aagcaaggaa aagaaaaaat gtcccaaaaa ttgatgccca gaaagttgat aacaatgccc
    26281 catatttttt tattacattg ctttttttac tccatacagt ataaaaagcc caggaaatag
    26341 cagaaaaaag tgcaagtaaa ttaccaataa aagacatatc aaaagaaaac tttccatctg
    26401 ttattacaaa ataacatcca ataaaaccaa gaataattcc aactatctca tttattctta
    26461 ctttttctct taaaaacata taagcaagaa ttccgataaa aatagagttt gaattcataa
    26521 gaatacttga agaaattgag gtggtaaatt taagagagta aaaaatgaaa atactcatcc
    26581 caaaaattcc tgtcaaacct aaaattataa aagatggaat atcatcttta ttgattttaa
    26641 attttattct atttaaagaa agataaataa aaagaaaaat tgaagcaaag aaaaatctta
    26701 aaaaagaaag taaaattgga tggcaacctt ctttaacagt tatctgccca aaaacaaaca
    26761 cagttcccca tgttattaca cttattatag ccgataaaat ttccctcatt tttactcctt
    26821 ttcttta
//
LOCUS       DAOOVW010000022        26017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_1263_length_26017_cov_9.018103, whole genome shotgun sequence.
ACCESSION   DAOOVW010000022 DAOOVW010000000
VERSION     DAOOVW010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 26017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26017
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1263_length_26017_cov_9.018103"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PLW95_04340"
     CDS             <1..237
                     /locus_tag="PLW95_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21892.1"
                     /translation="HKEDGLRGYLLLIFITLVVYALLKECIAGKYTVEEMLLKMRNLK
                     CKIYEDEIIIPEQTKQQKEITETLNIIMPKNMGI"
     gene            306..641
                     /locus_tag="PLW95_04345"
     CDS             306..641
                     /locus_tag="PLW95_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21893.1"
                     /translation="MNMKIMKILILLTITLFIFAGCSKKKKEEELTPLNAPAKYGETM
                     GRAMKKAEGMQDVLNLKNEINTFLIQEGRYPQSLQELVDKGYIKEIPKSPENMQFVYD
                     PKTGTIDVR"
     gene            655..1188
                     /locus_tag="PLW95_04350"
     CDS             655..1188
                     /locus_tag="PLW95_04350"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOV21894.1"
                     /translation="MDFYEVIKTRRSVRNYKDESISEDVLKRVLNAARIAPSGSNRQP
                     WQFILIRETERKKKMVKLCEGQKFVAEVPVLIVACGENIHYNRGNWMGDYSMLVDVAI
                     AVDHLTLAARAEGLGTCWIGSFDNEGIKKFLEIPEDINVVAVIPIGYPLYSDVFKENN
                     NRKTLEEIVFYEKWGNK"
     gene            1199..1273
                     /locus_tag="PLW95_04355"
     tRNA            1199..1273
                     /locus_tag="PLW95_04355"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1231..1233,aa:Gly,seq:ccc)
     assembly_gap    1319..1418
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1886..2353)
                     /locus_tag="PLW95_04360"
     CDS             complement(1886..2353)
                     /locus_tag="PLW95_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21895.1"
                     /translation="MRKRENNEFRSLKYVFFESVIFPFILLIIALYLKKNGFKHFVPD
                     YEEGMARIVQYILFFSGLFIFFFCEGISDFIVKRIFIKENEQKNFYSYYAYTIIMLWT
                     LNLITIVGFIGFLICGNITWLSVFLILNISMVYKYFPSKKHLENLISKLEKKQ"
     gene            2461..2856
                     /gene="speD"
                     /locus_tag="PLW95_04365"
     CDS             2461..2856
                     /gene="speD"
                     /locus_tag="PLW95_04365"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:TIGR03330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HOV21896.1"
                     /translation="MKKELYLVSPVGTHLIIDLYDCRCDFLDNSEKIQNLLIEVAKIS
                     NTTILSVKVHKFTPMGVSGYVLISESHISIHTWPEFNYASVDIYTCGDNSLPTKGFEC
                     IVEKLNSQKPTILKFERGFYAKKFKHDYG"
     gene            2825..3667
                     /locus_tag="PLW95_04370"
     CDS             2825..3667
                     /locus_tag="PLW95_04370"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HOV21897.1"
                     /translation="MQKNLNMTMVDWYSEGIGLCYVVEKKILEKQTKYQKIEICISKQ
                     FGKLLFIDGNLQSVEKFEENYHEILVHPAIFPHPDCKKVLIIGGGEGATLREVLKHPV
                     EKTKMVDIDGEMIEIAKNYLKYDRGAFCDKRTELIIDDGVKFIKKEEEIYDVIIVDAT
                     DPSNNSHYLYTTDFYIKVFLRLNKFGIFTTYAGGGYYFQPEILKMIYKNMKNVFDMVK
                     IYSFPIAGFLPGFSFIIGVKGNINIENIPEIKTKIELKYYSPEIHHHLFVLPYYFEKE
                     LKIL"
     gene            complement(3675..4997)
                     /gene="lpdA"
                     /locus_tag="PLW95_04375"
     CDS             complement(3675..4997)
                     /gene="lpdA"
                     /locus_tag="PLW95_04375"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOV21898.1"
                     /translation="MEKYDVVVIGGGPAGYPCAIKCAQNGKKVCLIEEMAIGGVCLNR
                     GCIPTKSLYTISKEIEKSKFTSIKKETSFSWQGILSEILTDVVMRLRAGVNMLLKSYG
                     IEVIAKKKGEIIDNNIVRIENEEIYGENIVIATGSLPFVPDCFKNDERVISSEQLWKL
                     NELPESIGIIGGGFIGCEYASILSKFGVEVTIYEMMDALLLGKDKEITNYLKNSFEKR
                     GIKINLGKPIKNSEEIKEEKIIVAVGRKPNLPQIPEEIEKDNGWLKTDEKMKTNIKNI
                     YAIGDINGKFPLAYVATKEGEISAENISGKEVTINYDIVPEVIFTDPQIGVCGLTEEQ
                     AKAKGINVSIGKFPYNALGRACADKKTDGFVKVISNSDTDEIVGIHIIGELATELVSF
                     STLTISNGLKIKDVEKIFYCHPTFSEGIMEGIMDLKRKSVHLPPKKST"
     gene            complement(4984..5547)
                     /locus_tag="PLW95_04380"
     CDS             complement(4984..5547)
                     /locus_tag="PLW95_04380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOV21899.1"
                     /translation="MEKIIFLDRDGVISIFTPNDFIKKWEEFKFLPDAIEGLKILTDA
                     GYKIVIISNQSGVGKKIFTEEDLNEITENMKKILFTNGIEIYRVYYCTHTEKDNCNCR
                     KPKPGLFLKAREEIGNIDFSKTFFIGDSEKDVIAGKTVGAKTILVLSGQTKSKKETEK
                     WEIKPDYIFKNLKESAEFIIGRKYGEI"
     gene            complement(5572..6597)
                     /gene="argC"
                     /locus_tag="PLW95_04385"
     CDS             complement(5572..6597)
                     /gene="argC"
                     /locus_tag="PLW95_04385"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013866747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOV21900.1"
                     /translation="MDKKIKVGIFGISGSAGEKLTEILTFHPLVEITAGYVASDEKQQ
                     NIEEIIPKLKGIINFECKNKIDWEFIKENCDLVFLSLPHSVSMNYVPELLKLGKKIID
                     LSADFRFKDISVYEKWYVKHICPEFINNVVYGLPELYRDEIKKTSFIANPGCYPTSVI
                     LGLLPLIKNNLIEENIIANSVSGVTGAGKKPSETNIFIQCNENISAYKIGSHRHQPEI
                     EEILKSVGGKNYKVLFVPHLAPYDQGILSTIYIKTNRMLEEKEITNLYNEFYKNEPFI
                     RLMDYGNCPSVKNVRDTNFCDIGFKIIENNIVIMSAIDNLVKGASGQAIQNMNLMYGL
                     KETLPFL"
     gene            complement(6582..6977)
                     /gene="rpsI"
                     /locus_tag="PLW95_04390"
     CDS             complement(6582..6977)
                     /gene="rpsI"
                     /locus_tag="PLW95_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019037642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOV21901.1"
                     /translation="MEKGNLTIAVGRRKTSRAIVKFKEGKGEIKINNKEISTYFPLFY
                     QKEEVLKPLSVTDTKDKFDINVKVEGGGITGQIDATKLAIARALIKYDPSLTPKLREF
                     GLLTRDPRMPERKKYGQKKARKKFQWTKR"
     gene            complement(6988..8517)
                     /gene="xylB"
                     /locus_tag="PLW95_04395"
     CDS             complement(6988..8517)
                     /gene="xylB"
                     /locus_tag="PLW95_04395"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HOV21902.1"
                     /translation="MKYLLGIDIGTSGAKIIIIDEKAIIKNSETIEYPVFTPKTNWAE
                     QNPLDWKQATLTGIRKIIEKSDINPSDIKAVGLTGQMHGSVFLDKNFKVIYPAILWCD
                     QRTTKECDEINEIIGKKRIFEITSNPVLTGFQAPKIIWLRKNKPSIYKKVRKILLPKD
                     YIRFILTGVFATDVSDASGTSLFDVKRRKWSEEIIEKLKIPIEFLPEVFESEDITGYI
                     TKETSNITKLKEGTPVVAGAGDQAAGGIGNGIVEEGKVSITIGTSGVVFAHSEEVVVD
                     PEGRLHTFCHAVRGKWHLMGVMLSAGGSLRWVRDTFCNEEIQQAKIKNLDTYDIMTEK
                     SKKVPPGCEGLIFLPYLAGERCPYPDPDAKGVFFGLSLKHKKEHIIRSVIEGATFGLL
                     DSIEIMKEIKLPLGEKYIVSGGGGKSDFWCSVMPDIFNKKITKLSNQEGPSYGAAILA
                     GVGIGIFKDAKSACKKFIKEKDVFKPDIKNVKIYKSAYKIYRKLYSSLKNDFKLLKEI
                     S"
     gene            complement(8514..9617)
                     /locus_tag="PLW95_04400"
     CDS             complement(8514..9617)
                     /locus_tag="PLW95_04400"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HOV21903.1"
                     /translation="MEIIVKNCKRKFYNGIIKFDFDGEGIYVDKKENIYFQVDEEEKK
                     MFFIDEFEENEEKNLNLEKIENSPEIVKVVENTKNEEVNVFINEELFTTYHYSEKVTR
                     PFLNPVIGPDGKSVVRPPASEGNTENFDHIHHRGILVAHGDVNGTDNWSEEEGHGKTI
                     HKKFISLISGPVFGKIHSLSDWVNNKGNKILQEERIITIYNLPKNIRIIDHKIILKAT
                     EGEVVFKDTKESGLLSIRVYPSMEVRNGGTMKNSYGAIGEEECWGKRANWCDYYGKID
                     ETSCGISVFDFTENLRYPTYWHIRDYGLFTANFFGISDFSGDKRKSGTYILPAYQDLK
                     LLHRIYIHSGTTEEGKVKEMYLNFIYPPKIEIK"
     gene            complement(9630..10667)
                     /locus_tag="PLW95_04405"
     CDS             complement(9630..10667)
                     /locus_tag="PLW95_04405"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOV21904.1"
                     /translation="MEKVKIAITGLGRSGWNIHAHLISQLPEKYEIIAVSDPDENRRK
                     QAEEKFDCKSYEKFEDMLNNDEIEVVVVATPSHLHSSYSISALKKGKNVICEKPMATN
                     LEDADKMIEVAKKEGKILTIFHNRRYSPDFLKVKEVIDTGVLGRIVLIKMMWHSFARR
                     WDWQTLKKYGGGTLNNTGPHPVDQALQFFGEKEDPEIFCHMERTLTLGDAEDHVKIIF
                     KGKISPMIDLEITSCCCYPEEPYLIMGTCGGLKGNYTKLQWKYFKVEKLPERKVQTQP
                     TPDRSYNSEEIPWEKEEIWELPQNQHDYQLNFYIDLYETLKKNKPLKITPESARRVMW
                     AIEKCHKIAGI"
     gene            complement(10679..11488)
                     /locus_tag="PLW95_04410"
     CDS             complement(10679..11488)
                     /locus_tag="PLW95_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOV21905.1"
                     /translation="MKIGICNEMFENWDIEKIFYFIKEIGYEGLEIAPFTLGNSVDEI
                     EKELIEKIKFLSKKTDIEIIGTHWLLAKPEGLSVSTNDKSVREKTSSYLCQLVHFTAE
                     IGGKIMVFGSPKQRNIEKNQTYDEVKKNFIECLLKPLEVAEKENIYICLEPLAKSETN
                     FINTAKEAIEIINEVGHPNLKLHLDVKAMCDEGKPIPEIIKESRRYLKHFHTNDKNLL
                     GPGFGDVNYEPIIETLKEIGYNGYLSVEVFNFSPGAEVIARKSIEYLRKFL"
     gene            complement(11485..12555)
                     /gene="ftsZ"
                     /locus_tag="PLW95_04415"
     CDS             complement(11485..12555)
                     /gene="ftsZ"
                     /locus_tag="PLW95_04415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOV21906.1"
                     /translation="MIQMIQQTPEEGIINIKVVGVGNAGGNIISRIHDKIEGVSFVLF
                     NTESNALKQAKGDIKIQIGEKTTQGIGAGKDPEKGKFAANEDREKIEEIFKNTNITFL
                     VAGLGGGTGTGSSPVIAKIAKDLGSIVIALVTTPFEFEGEKRMEHAINGIEVLLNNVD
                     TLIHISNQKLYEIVDNTVSFASAFGKIDEIIERTISSISDLINKPKLLDIDFADICSV
                     VQNGGKGIVGIGYGEGEKRMEESTKQILENPLVEKSEIMEAKKILISMTGGSDLTLIE
                     VGETMKLIQSKIFSPFRVLGVAIDENLKNSVKITLLATGIGKKVEIKKPKEEDKLPLG
                     EIEKPATNIDTPAILRKKGGSL"
     gene            complement(12552..13823)
                     /gene="ftsA"
                     /locus_tag="PLW95_04420"
     CDS             complement(12552..13823)
                     /gene="ftsA"
                     /locus_tag="PLW95_04420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOV21907.1"
                     /translation="MNENLITGLDIGSGKILGITGYLSDTGIDILGTEIVEVPENFIT
                     KGRITDIEGVTNLLFDVFDSLSEQVKERISYVTIGIGGGYVKGSIYSKKIEITPNGRE
                     INKNDIQFLKKEIKNSIMAGKEVGEKILTILPQFYIIDGHNITTKLPIGMHGNTLEMK
                     VLAISVEINPVKDIGHCIESAGGQIEHIFPHAWASGEATLTEEEKEMGCLLIDMGKGT
                     TDIAIYFKNTITEVASFRIGGWYIDNDLSAVLYTPIELAEELKINYGYCNYLKMKENK
                     DPKLLEEIEISSPSGKIIGKVTVEKISEIVYARVTEILYDYVKPVVVKNSLSVSLAGG
                     VIITGGCSKLKGIIELSSDIFKLPVRIGVPIRTYNLDKNFQKPEYSSAFGLITLASKQ
                     LKERQKSFYDKLKETFAKIKKNFKKGLRKIK"
     gene            complement(13820..14593)
                     /locus_tag="PLW95_04425"
     CDS             complement(13820..14593)
                     /locus_tag="PLW95_04425"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOV21908.1"
                     /translation="MEEKTERRIREIRQRIWEKRKNKIKIIFIIILILSLIYLSFKIK
                     SSTIKFLWQLDTFKLKEVEIVPDKAKFYIEPVLELEKDKNLLFIDIDDLRQMILNIPE
                     VENCKIKKIYPDKLYIDISLRKPFVSVIYNERKYIIDKNGVVLSDIEEEKNYIDIYGI
                     KIEQNCVNETDRWKLNILDEIKKWYNYFNLRKFFLVNQINFISPNEIILYTDKGIIKM
                     KKSDIKSQMEKISILLQELQGNIERWEYIDLRYKNIYKK"
     gene            complement(14566..15492)
                     /locus_tag="PLW95_04430"
     CDS             complement(14566..15492)
                     /locus_tag="PLW95_04430"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOV21909.1"
                     /translation="MKIIVLAGGKSPERDVSLMSGKQVYNALKRKNYSVILLDPSKKT
                     FLKKLIEFKPDCVFIVLHGGKGENGVIQGTLETLNIPYTGSGVLSSAMCMNKIITKKF
                     LKFHKIPTPPFVVVEKNKKFHLPFSFPVVVKPANLGSTIGIRIVKKEKELKKAVKECF
                     NLDNDVFIEKFIEGKEITIGILGNEKIRVLPAIEIRTKTGFYDYKAKYTPGESQHIIP
                     PEIPEKFLKNAYNNAKKVYKIMRCSGFARMEIMINKNGVPYILDVNTIPGMTKTSLLP
                     DAAKYAGINFDDLCEQILNYAIEKWKKKQKEE"
     gene            15621..16379
                     /locus_tag="PLW95_04435"
     CDS             15621..16379
                     /locus_tag="PLW95_04435"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HOV21910.1"
                     /translation="MEKKIKVIEKIMGIFEVLADRGKEGCSVSEIAKKLNLKLTTTHS
                     LVSTLFSLGYLEKNIQTRKYKIGEKLLNIFLPFANRNILLKVSEPVMKALAEEIKESV
                     VLAIFWKGERYTIATASYDKQLISVNLNQFIYSSCYSQATGRVLLSYLDKKVVNEYIK
                     RNGFPKEKWNNIRNYNDLQKELKKIKEKKIEIIERETVVAIAVPIFSPDGKVCASLGV
                     YLPRIRFKGQRKEKIIKEIKKAGEQITLNLRGEK"
     gene            16376..17878
                     /locus_tag="PLW95_04440"
     CDS             16376..17878
                     /locus_tag="PLW95_04440"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOV21911.1"
                     /translation="MIKAENGRIEFKENLNVKYEVDVVVVGGGTAGFAAAISSAENGM
                     KTMLIEKQGALGGLVTLGLVCYMASYPEGIGKRLLERLEDGKGIAERICDPEKTKYIM
                     EQMAIEAGVKILYWTYVIDSIVEGNEIKGVVIQNISGRSAILAKRVIDCSGDAEVSYF
                     SGVPVEEGWELMDGYNQAVSLDFVLNNADTTKFQPRDFYSTIQMKIEEAARKGKLPRL
                     VEKGYLGPFPGRPSDRGEVYACTAHSRKCKATDAEDLTRIAIEQRKQIQQLVEFYRET
                     VCGFENSWLSYTATILGVRESRRIVGEYKFTGEDLALAKKFPDAIARDTHGFDIHNPI
                     NDLPHIKHTHFKEPKEPAFCTKEPRQDGRVCCVKNNPKGEYRAYLKPGQYYEIPYRCL
                     VPVKIDNLLVAGRCISADFEGSAGTRLIMTCITIGHAAGTAASLSIKENVAPRKLNTN
                     LLRDKLDDEGVNLKKEPPVYVKGGPRKPIPENAEFYVYTGDGEDEIRIKE"
     gene            17908..18801
                     /locus_tag="PLW95_04445"
     CDS             17908..18801
                     /locus_tag="PLW95_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21912.1"
                     /translation="MITSEEVKEVGKRCGADLVGIASMDRFEGAPKQMDPRYINPDAK
                     ALIVIATRIPRGTLRGIEEGTYFLSYSSMGYGGINSVYNPMILWQLVRYIENEGYEAT
                     PIPNDYYNWPAIDYHSGVIKENWSRPVSPEKPAPDVFIHFRIAAFCAGLGEIGYSKVF
                     LTPEFGPRQRFACILTDAPLQPDPIYEGPKICDRCMLCVKECSGKAISSKEVVKIKVA
                     GRQIEWGKLDIDACSKGFQGGNKKFNPFIGEPSEVISYSRALEGGKGCIRACMIHLEQ
                     EGKLKNKFEEPFRKRKPWELK"
     gene            18844..>19378
                     /locus_tag="PLW95_04450"
     CDS             18844..>19378
                     /locus_tag="PLW95_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HOV21913.1"
                     /translation="MITSEEVKEVGKRCGADLVGIASMDRFEGAPKQMDPRYINPDAK
                     ALIVFGFRIHRGLLRGIEEGTYFSGYTSMGYGGINTVFAPLVLRQVCNFIEDNGYEST
                     AYPNPLVAVAVDIATGEFKKGWSRPVSPEKPAPDVFIHFRIAAFCAGLGEIGYSKVFL
                     TPEFGPRQRFACILTDAP"
     assembly_gap    19379..19478
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <19479..19821
                     /locus_tag="PLW95_04455"
     CDS             <19479..19821
                     /locus_tag="PLW95_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21914.1"
                     /translation="EVVKIKVAGRQIEWGKLDVIKCSNAYTGAVKETNPFLPEDLPDK
                     FEEWSKYYVDANPHKNEFVKWYGGIGGHNPGLEGGRGCLRACMIHLEQEGKLKNKFEE
                     PFRKRKPWELK"
     gene            19864..20196
                     /locus_tag="PLW95_04460"
     CDS             19864..20196
                     /locus_tag="PLW95_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21915.1"
                     /translation="MITSEEVKEVGKRCGADLVGIGSMDRFEGAPKQMDPRYIMPEAK
                     SIIGIGFRIHRGLFRGIEEGTFFAAYTAMGYATINDGYAPKVLREVGNFIEDNGYETM
                     LYQNTVFG"
     gene            20283..20855
                     /locus_tag="PLW95_04465"
     CDS             20283..20855
                     /locus_tag="PLW95_04465"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOV21916.1"
                     /translation="MGEIGYSKIFLTPEFGPRQRFAFIITDAPLQPDPIYEEPKICDR
                     CMLCVKECPSGAISRKETVEVKVAEKTLEWGSLDELKCAACYQAGTPEYSPFMPEKVA
                     KTLEGIFKYQSGSESKGMTDYTGDVWAYLREEFPYIRNAWESYHHPANICGAKGCIRA
                     CMIHLEQEGKLKNKFKQEFRKHQPWVIKRK"
     gene            20834..21868
                     /locus_tag="PLW95_04470"
     CDS             20834..21868
                     /locus_tag="PLW95_04470"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOV21917.1"
                     /translation="MGNKTEIKFSVGYQLREDKLFKDIVERYKEKIEEVYFSWLNLPS
                     GRGPIAEKVGYINWEGQKQLEYELNCIKKMGIKLGLLLNASCYGGFNLSVSLANTVVS
                     TIEYLQENIGIDIVTVFSPVVAYIIKKNFPGIKIRGSVNLRIGTVEGMEYVSDLFDSF
                     VMQREYNRDFETIEELNQWCNKNGKTLCILVNSGCMNFCAVQLFHDNTISHEIDVYTR
                     GNITEEIPGNCWSYYKKRENWRKLLQNSSWIRPEDIHNYKEKFSHFKLATRINQEPEK
                     IIKAYCNEKFDGNLLDLFEPSHTKLLFPYIIENTKFPEDWFQKISSCKRNCDKCSYCK
                     NILENILVKF"
     gene            21881..24112
                     /locus_tag="PLW95_04475"
     CDS             21881..24112
                     /locus_tag="PLW95_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21918.1"
                     /translation="MDEKKKLTEEVKRYAKSLGVDMVGIAPVERFKNAPLRMSPKGLL
                     PESKSVIVLGIHHLDCAVELGGYPTPHDIGPYGTQSSVMNPMLDDFAFKVARFLEDKG
                     YKTLPIPVTNIWRYNPYKDLDVSFAPDLAHRYAAVAAGLGEIGWSGLFLSSEFGPRQR
                     IISIITEVELIPDPMYEGEKLCDKCMECVKHCPTDAFRKEVKKINKIEIGNKIFEFPD
                     TNKWRCAWAENFALNLSLPIPDKVDEKVILENLEKYGRRGGEEGCCLKYCMVKEKRYY
                     QLNYTDAPRRKKEITKKADVIKEEILKMVQDEFVDVIAIATVEKFEDSHFFHPEFHLP
                     DAQTVISIGIKIENETLEISSAVRRTIDFLSYKISHLLDVSGYSSITSGNVPHLTIGQ
                     KLTGKLKNYYFSTILTSAKISEFNWEKRTNKKKLKKEDIKNFCLEQKVDIVRFFTTSR
                     YENFMKKIESYFSDIYYEIEDKGGIQSAFVPEIKEKKIEIKKVSDYLEEGKSVIAIGL
                     HFPDACLDTAKITPAETVGPYAYAQYETIFLLRDIGLNVVKFLQNNGYKATLKESITG
                     FYSFVKNSRGYLPDMRTGNIEAFLSGAGYIGENGVIITEKFGQRQRFIFIITDADFEN
                     DPLYNGENFCKKCSHPCITSCPTKAIKKGNTILKFEGREFKLPDLDIYRCDWAKRYGL
                     SGKEGPSFYGIEPNVSIPTKITPEKIAECLINTKWGVQKLHINICEECIRNCSYKGEK
                     NGK"
     gene            24102..24971
                     /locus_tag="PLW95_04480"
     CDS             24102..24971
                     /locus_tag="PLW95_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21919.1"
                     /translation="MVNKKELKEYAVEVKADLIGITSMDRFKGIDKQHHPLSIFPEGK
                     SVIIVGKRITRGAIRGIEEGTQFSIYSQYGCSWLNDRFLALTTFKISEFLEDNGYEAI
                     PIVNLPSEISPMGIPVKKGKPSPNVLIDIEDAAIRGGLGEIGYCGIFLTEEFGPRQRF
                     QVIITDLEIEPDPIKKENVCDLCKECMKICPLRAIENEEKEIEICGKKMIVADINYEI
                     CKICKNGVFPNMYYENAKPDRIAALCSRTCLVHLEKTKRIKNLFNENFRNRTTWKIDK
                     TGEIMLDEGRDIE"
     gene            24968..26014
                     /locus_tag="PLW95_04485"
     CDS             24968..26014
                     /locus_tag="PLW95_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21920.1"
                     /translation="MKKLTKETIREMGYKLGLDLVGFANIERFKDAPENMHPKGIFPE
                     AKTVIVVGNRNLRGAWRGIEEGTNWVSYTYFDYSGLANTFFIQEPLYQLACFIEDSGY
                     EAVPYYSGVPEAQSPSLKPLRENQPSPDVHLAIRIAGICAGLGEIGWSKVFLTKKYGP
                     RQRLHAIITDLEVEPDPLVKPLTICKRCMKCVSDCPSHAIPHIKENKKISINIENYTY
                     EWADIDMGKCTLSYHGGDSRVSPFIHKDFPGWHIDARKQQFSEECAYKFCWTLSTGRW
                     RKTKEFPSGYIIEGHGYLQKWGVGGSYGICGSRSCMRSCFNYLEEKNFIEQKFHNGSF
                     IKRKRWLLPYSVKK"
BASE COUNT         8842 a         3826 c         4126 g         9023 t
ORIGIN      
        1 cataaagaag atggcttgag aggatattta ttattgatat ttataacttt ggttgtttat
       61 gctcttttga aagaatgtat agcggggaaa tatacagttg aagaaatgtt attgaaaatg
      121 agaaatctaa aatgtaaaat atatgaagat gaaataataa ttcctgaaca aacaaaacaa
      181 caaaaagaaa ttacagaaac attaaatatc ataatgccta aaaatatggg aatttaggta
      241 aaattaaatc tatttaaaac tttttataat ttatgtataa tttttacaaa agaaaagagg
      301 ggtttatgaa tatgaaaata atgaaaatat taatcctatt aacaattaca ctttttattt
      361 ttgctggctg tagtaaaaag aaaaaagaag aagaattaac ccctttaaat gcacctgcaa
      421 aatatggtga aacaatgggt agagcaatga aaaaagcaga agggatgcag gatgttctta
      481 acttgaaaaa cgaaataaat acatttctga ttcaggaagg aagataccca caatcattac
      541 aggaacttgt tgataaagga tatataaaag aaattccaaa atcacctgaa aatatgcaat
      601 ttgtttatga cccaaaaact ggaacgattg atgtaagata aaaaggataa aaaaatggac
      661 ttttatgaag ttataaaaac aagaaggagt gtaagaaatt ataaggatga atcaatttca
      721 gaagatgttc ttaaaagagt tttaaatgct gcaagaattg ctccaagtgg aagtaataga
      781 caaccatggc agtttatttt aataagagag acagaaagaa aaaagaaaat ggttaaactt
      841 tgtgaaggac agaaatttgt tgcagaagtt ccggttctta tagttgcctg tggggaaaat
      901 attcactaca atagaggaaa ttggatgggt gattatagca tgcttgttga tgtggcaatt
      961 gcagttgacc atttaactct tgcggcaaga gcagaaggac ttggaacatg ctggattggt
     1021 tcttttgata atgaaggaat taaaaaattt cttgaaatac cagaagatat aaatgttgtt
     1081 gctgtaatac caattggata tcccctttat tctgatgttt ttaaagaaaa taataataga
     1141 aaaacactgg aagaaatagt tttttatgaa aaatggggaa ataaataaat aggtaaaagc
     1201 gggattaact cagcggtaga gtgcgagctt cccaagctcg aggccacggg ttcaaatccc
     1261 gtatcccgct ccatctctaa ctttgtaagg gagttgaacc ttttgggttc aaaccgacnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntc tctaactttg taagggagtt
     1441 gaaccttttg ggttcaaacc gactaaagta gcttaccttg tatctcagag ttggtgtgtc
     1501 atcgtctccc agatagcaca actgctaccg acaaaagtag caaagatcag caaatcagag
     1561 ttggtgcttt gttttcccaa aaaacttccc cctttaacca aaggggggag ggggatttaa
     1621 taaaatcccg taagcgtaac ttatcgcttc ggcgcctgcc cgtctgagca ttagcgaagg
     1681 agggtaggat ggtctgtaag caatactcta aacccttctg tgtaagacaa tccatcccgt
     1741 ccttcctttt acaaaggaag gagtgagttg gcgtggcaga aaaaatttct tttcaattta
     1801 gtaagcacaa tccccttggg cgtagttcta cagaacaatc ccttgggcgt agttctatag
     1861 aacgagcccg tatcccgctc taattttatt gtttcttttc caatttactt attaaatttt
     1921 caagatgttt tttagaaggg aaatatttat agaccataga aatattcaaa attaaaaaga
     1981 cagaaagcca tgtaatattt ccgcatatta aaaagccaat aaaacctaca attgttataa
     2041 gatttaaagt ccagagcata attattgtat atgcataata agaataaaaa tttttttgtt
     2101 cgttttcttt tataaatatc ctttttacta tgaaatcaga aatcccttca cagaagaaaa
     2161 aaataaaaag gcctgaaaaa aacaaaatat actgcacaat tcttgccata ccttcttcgt
     2221 aatcaggaac aaaatgctta aaaccatttt ttttgagata cagtgcaatt atcaaaagta
     2281 taaaaggaaa tataactgac tcaaaaaaaa catattttaa acttctaaac tcattatttt
     2341 ctctttttct cattttattg acacctttct ttttttaaat tactatttaa ttataaactt
     2401 taaaaggtgt agtgtaaagt tccttctttt aagatggagg aaaacaaaaa ggagatagaa
     2461 atgaagaaag aactttatct tgtgtctcca gttggtaccc accttataat tgacctttat
     2521 gattgtagat gcgattttct tgataattca gaaaaaatac aaaatctctt aatagaagta
     2581 gcaaaaattt ctaataccac tattttaagt gtaaaagttc ataaatttac ccctatggga
     2641 gttagtggtt atgttcttat aagtgaaagt catataagta ttcatacatg gccagagttt
     2701 aattatgctt ccgtagatat ttatacatgt ggagataatt cattaccaac aaaaggattt
     2761 gaatgtatag ttgaaaaact taattcacag aaaccaacaa tacttaaatt tgaaagggga
     2821 ttttatgcaa aaaaatttaa acatgactat ggttgactgg tattcagaag ggattggtct
     2881 atgttatgtg gtggaaaaga aaattttaga aaagcaaact aaatatcaga aaattgaaat
     2941 ttgtatttca aagcagtttg gtaaactctt atttatagat ggcaatttac aatcagttga
     3001 aaaatttgaa gaaaattatc atgaaattct tgttcatcct gcaattttcc ctcatcctga
     3061 ttgtaaaaaa gttcttatta ttggtggtgg agaaggtgca actttaagag aagttttaaa
     3121 acatcctgtt gaaaaaacaa aaatggttga tatagatggt gaaatgattg aaattgcaaa
     3181 aaactacctt aaatatgata ggggggcttt ttgtgataaa agaactgaac ttattattga
     3241 tgatggagta aaatttataa aaaaagaaga agagatatat gatgtaataa ttgttgatgc
     3301 gacagaccct tctaataatt cgcattattt atatacaact gatttttata taaaagtttt
     3361 tttacggtta aacaaattcg gaattttcac aacttatgct ggtggtggat attattttca
     3421 accggaaatt ttaaagatga tatataaaaa tatgaaaaat gtttttgata tggttaaaat
     3481 atactctttt cctattgcag ggtttcttcc aggattcagt tttataattg gagtgaaagg
     3541 aaatatcaat attgaaaata tccctgaaat aaaaactaaa attgaattaa aatattattc
     3601 accagaaatt caccaccatc tttttgtgct tccttattat tttgaaaaag aattaaaaat
     3661 tctttaaaaa agttttaagt tgattttttt ggtgggagat gtacactttt tctttttaga
     3721 tccataattc cttccattat tccttctgaa aaagttggat ggcagtaaaa aattttttca
     3781 acatccttta ttttaagtcc attactaata gttagggttg aaaaagatac aagttctgtt
     3841 gctaattccc caataatatg aataccaaca atttcatctg tatcagaatt ggaaattact
     3901 tttacaaatc catctgtttt tttatctgca catgcccttc ctagagcatt atatggaaat
     3961 tttccaattg aaacatttat tccttttgcc tttgcctgtt cctctgttag cccacaaaca
     4021 cctatttgag ggtcagtaaa aataacttct ggaactatat catagttaat agtaacttct
     4081 tttcctgata tattttcagc agatatttct ccttcttttg tagcaacata ggcaagtgga
     4141 aatttcccat ttatatcacc aattgcataa atatttttaa tgtttgtttt cattttttca
     4201 tctgttttga gccatccatt gtctttttct atttcttctg gtatttgagg taaatttggt
     4261 tttctaccaa ctgcaacaat aattttttct tcttttattt cttcagaatt tttaattggt
     4321 ttgccaagat tgatttttat tcctcttttt tcaaaagaat ttttaagata gttggtaatt
     4381 tctttatctt ttcctaaaag aagtgcatcc atcatttcat aaattgtaac ctcaactcca
     4441 aatttactta aaattgaagc atattcacat ccaataaatc caccaccaat aattccaata
     4501 ctttctggta gttcattaag tttccataat tgctcactgc ttataactct ttcatcattt
     4561 ttgaaacagt caggaacaaa tggcaaactt cctgttgcaa ttactatatt ttctccataa
     4621 atttcttcat tttcaattct cacaatattg ttatcaataa tttctccttt ctttttagca
     4681 ataacttcta ttccatatga ttttaagagc atattaacac ctgctcttaa tctcattaca
     4741 acatctgtta aaatttctga aagaattccc tgccatgaaa aagatgtctc ttttttaatt
     4801 gatgtgaatt tacttttttc aatttctttt gaaattgtat aaagagattt tgttggaata
     4861 caacctctat ttaaacatac tccacctata gccatttctt caattaaaca gacttttttc
     4921 ccattttgag cacattttat tgcacatggg tatccagcag gacctccacc aattacaaca
     4981 acatcatatt tctccatatt tcctcccaat tataaactct gcactttctt tcaaattttt
     5041 aaaaatatag tctggtttta tttcccattt ttctgtttct ttctttgatt ttgtctgtcc
     5101 tgaaaggaca agaattgttt ttgcaccaac tgtttttcct gctataacat ctttttctga
     5161 atctcctata aaaaatgtct tagaaaaatc aatatttcca atttcttctc tcgcttttaa
     5221 gaaaagtcct ggttttggtt ttctacaatt acaattatct ttttctgtat gggtacaata
     5281 ataaactcta taaatttcta ttccattggt aaatagtatt tttttcatat tttctgtgat
     5341 ttcattcagg tcttcttctg taaaaatttt ctttcctaca cctgattgat ttgatattat
     5401 aactatttta tatccagcat ctgttaaaat tttcaaccct tcaattgcat ctggcaaaaa
     5461 tttaaattct tcccattttt taataaaatc atttggagta aaaatagaaa taactccatc
     5521 tctatctaaa aaaattatct tttccattac aatcccgtgg tttataaacg gttatagaaa
     5581 ggggagggtt tctttaagtc catacatcaa gttcatattt tgaattgcct gaccgcttgc
     5641 tcccttaact aaattatcaa ttgctgacat tataacaata ttattctcaa taattttaaa
     5701 gccaatatca caaaaatttg tatctctaac attttttact gatgggcagt taccatagtc
     5761 cataagacga ataaatggtt catttttata aaattcatta tataagttag taatttcttt
     5821 ttcttctaac attctatttg tctttatgta tatggttgat aaaatgccct ggtcataagg
     5881 agcaagatgt ggtacaaaaa ggactttata attttttccc ccaacacttt ttaaaatttc
     5941 ttcaatttct ggttggtgtc tatggctacc tattttatat gcggatatgt tttcgttgca
     6001 ttgaataaaa atgttagttt ctgatggttt ttttcctgct cctgttacac cacttacaga
     6061 atttgctatt atattctctt caattaggtt attttttata agaggaagta atcctaaaat
     6121 gactgatgtg ggataacatc cagggtttgc tataaaagat gtttttttaa tttcatctct
     6181 ataaagttca ggcagtccat aaacaacatt atttataaat tcaggacaaa tgtgttttac
     6241 ataccatttt tcataaacag aaatatcttt aaaacgaaaa tcagcactca aatcaattat
     6301 ttttttacct aattttaata gttcaggaac atagttcata gagacagagt gaggaagaga
     6361 taaaaaaact aaatcacaat tttcttttat aaattcccaa tcaattttat ttttacattc
     6421 aaaatttata atacctttaa gttttggaat aatttcttct atattttgtt gtttttcatc
     6481 agaagcaaca tacccagcag ttatttctac taatggatga aaagtaagta tctctgtaag
     6541 tttttctcct gcactcccac ttataccaaa tatcccgact tttatctttt tgtccactgg
     6601 aatttctttc ttgctttttt ctgtccgtac ttttttcttt caggcatcct cgggtccctt
     6661 gtaagaagtc caaattctct taattttggc gtaagagatg ggtcatattt tattaatgct
     6721 cttgctattg caagtttggt agcatcaatt tgtcctgtaa tccctccacc ttcaactttt
     6781 acatttatat caaatttatc ttttgtatca gttactgaaa gtggttttaa aacctcttct
     6841 ttttgataaa ataaaggaaa ataagttgaa atttctttat tgtttatttt tatttcacct
     6901 tttccttctt taaatttaac tattgctctt gatgtttttc ttcttccaac tgctattgtt
     6961 aaatttccct tctccataaa ttcctcctta acttatctct tttaagagtt taaaatcatt
     7021 ttttaatgat gagtaaagtt ttctataaat tttatatgca cttttatata ttttaacatt
     7081 ttttatatca ggtttaaata catctttttc tttaatgaat tttttgcagg cgctcttagc
     7141 atctttgaaa atacctattc caactcctgc aagaattgct gctccataag aaggcccttc
     7201 ctggtttgat aattttgtaa tttttttgtt gaaaatatca ggcataactg aacaccagaa
     7261 atcacttttt cctccaccac cagatacaat atatttctcc ccaagtggta attttatttc
     7321 tttcataatc tcaatggaat ctaaaagtcc aaaagttgct ccctcaataa cacttctgat
     7381 tatatgttct tttttgtgtt ttagagaaag cccaaaaaaa acaccttttg catcagggtc
     7441 aggatatgga catctttctc ctgcaagata ggggagaaaa ataagccctt cacatccagg
     7501 aggaactttt ttactttttt ctgtcattat atcataggta tctaaatttt taatttttgc
     7561 ctgttgaatt tcttcattac aaaaagtatc tctcacccat cttaaagagc cacccgcaga
     7621 aagcataact cccatgagat gccatttacc cctaactgca tggcagaagg tatgaagtcg
     7681 tccttctggg tcaacaacaa cttcctctga atgagcaaaa acaactccac ttgttcctat
     7741 tgtaatagaa accttgccct cttcaactat cccgtttcca attcctcctg ctgcctggtc
     7801 tcctgctcct gctacaactg gagttccttc ttttaatttt gtaatattag aagtttcttt
     7861 tgtaatataa ccagtaatat cttctgattc aaaaacttct ggtaaaaatt caattggtat
     7921 tttaagtttt tcaataattt cttctgacca ttttctcctt ttaacatcaa aaagagatgt
     7981 tcctgatgca tctgatacat cagtagcaaa aacgccagta agaataaacc ttatataatc
     8041 ttttggtaaa agaatttttc ttaccttttt gtagatagat ggtttattct ttcttaacca
     8101 tataattttt ggtgcttgaa aaccagtcag aactggattg gatgtaattt caaaaattct
     8161 ttttttacca ataatttcat ttatttcatc acactctttt gttgttctct ggtcacacca
     8221 caaaattgca ggataaatta ctttgaaatt tttatcaaga aagacagaac catgcatttg
     8281 tcctgttaaa ccaactgctt ttatgtcaga tggatttata tctgattttt caataatttt
     8341 tctaattcca gttaatgttg cctgtttcca atcaagagga ttttgttctg cccaatttgt
     8401 ttttggtgta aaaacgggat attctattgt ttctgaattt tttataattg ctttttcatc
     8461 tataataatt atttttgctc cacttgttcc aatatctatt ccaagtaaat atttcacttt
     8521 atttcaattt taggtggata tataaaattc agatacattt ctttaacttt accttcttct
     8581 gttgttccag aatgtatgta aattctatgt aaaagtttta ggtcctgata tgcaggtaaa
     8641 atatatgtac cactttttct tttatcacct gaaaaatcag aaattccaaa aaagtttgca
     8701 gtaaataatc catagtccct tatatgccag tatgttggat atctcagatt ttctgtaaaa
     8761 tcaaaaacac ttattccaca tgaagtttca tcaattttcc cataataatc acaccaattt
     8821 gctctttttc cccagcattc ttcttcacca attgctccat aagaattttt cattgttccg
     8881 ccatttctta cttccataga tggatagacc ctgatactta gaagtccact ttcttttgta
     8941 tctttaaaaa caacttctcc ttctgttgct tttaaaatta tcttatggtc aataattcta
     9001 atattttttg gtaagttata aattgtaata attcgctctt cttgaaggat tttatttccc
     9061 ttattattta cccagtcact taaagaatga attttcccga aaacaggtcc tgaaatgaga
     9121 gaaataaatt ttttatgtat tgtttttcca tgtccttcct cttcagacca attatcagtt
     9181 ccatttacat ctccatgtgc aactaatatt cctctatgat gaatatggtc aaaattttct
     9241 gtgtttcctt ctgaagcagg aggtctaaca actgattttc catcgggacc aataacaggg
     9301 tttaggaaag ggcgggtaac tttttctgaa tagtgataag ttgtgaaaag ttcctcattt
     9361 ataaaaacat taacttcttc atttttagta ttttcaacaa cttttactat ttctggggaa
     9421 ttttcaattt tttctaaatt caaatttttt tcttcatttt cttcaaattc atctatgaaa
     9481 aacatctttt tttcttcttc atctacctga aaatatatat tttctttttt atctacataa
     9541 ataccttctc catcaaaatc aaatttaatt attccattat aaaattttct cttacaattt
     9601 tttacaatta tttccatttc tctctccttt taaattcctg caattttatg acatttttca
     9661 atagcccaca taactcttct tgcactctca ggtgtaattt ttagtggttt gtttttcttt
     9721 aatgtttcat aaaggtcaat ataaaaattt aattgatagt catgttgatt ttgtggtaat
     9781 tcccatattt cctctttttc ccatggtatt tcttcactat tatatgacct atcaggggtt
     9841 ggttgtgtct gaactttcct ttctggtaat ttttctacct tgaaatattt ccattggagt
     9901 tttgtataat ttcctttcaa tccaccgcag gttcccatta ttaaatatgg ctcttctgga
     9961 taacagcaac aacttgtaat ttcaaggtct atcataggcg aaattttacc tttaaatata
    10021 atttttacat ggtcttctgc atctccaagt gttaatgttc tctccatatg gcagaaaatt
    10081 tcagggtctt ctttttcacc aaagaattgc agtgcctggt caactggatg tggacctgta
    10141 ttgttaagag tgccgccacc atattttttc agtgtttgcc agtcccatct tcttgcaaaa
    10201 ctgtgccaca tcatttttat taaaacaatt ctaccaagaa ctcctgtgtc aattacttct
    10261 ttaactttaa gaaaatcagg tgaatatctt ctattatgaa aaatagttaa aattttacct
    10321 tctttttttg ctacttcaat cattttatct gcatcttcca aatttgttgc cattggtttt
    10381 tcacatatga cattttttcc tttttttaat gcagaaattg aataagaaga atgaagatga
    10441 gatggtgttg caacaactac tacttcaatt tcatcattat tcagcatatc ttcaaatttt
    10501 tcataacttt tacaatcaaa cttttcttct gcctgttttc tcctattttc atcagggtca
    10561 gaaacagcaa taatttcata tttttcaggt agttgtgaaa taagatgagc gtgtatattc
    10621 catccacttc ttccaagccc tgtaattgct atttttacct tttccatttt ttctcccttt
    10681 atagaaattt tctcaaatat tcaatacttt ttcttgctat tacctctgct cctggtgaga
    10741 aattaaaaac ttcaactgaa aggtatccat tataaccaat ttcttttaat gtttcaatta
    10801 ttggttcata gttaacatct ccaaaaccag gaccaagtaa atttttatca tttgtatgaa
    10861 aatgctttag gtaccttcta ctttctttta ttatttctgg tattggtttt ccttcatcac
    10921 acattgcttt aacatctaaa tgaagtttca aatttgggtg gcctacctca tttattattt
    10981 ctattgcttc ttttgcagta tttataaaat ttgtttctga ttttgccagt ggttctaaac
    11041 aaatataaat attctctttt tctgctactt ccagtggttt taaaaggcat tcaataaagt
    11101 tttttttgac ttcatcatat gtttggtttt tttctatatt tctttgcttt ggagaaccaa
    11161 ataccataat ttttccccct atttctgctg tgaaatgaac aagttgacat aagtaagaag
    11221 atgttttttc tcttactgat ttatcatttg ttgaaacaga caagccttct ggttttgcaa
    11281 gcaaccagtg agttccaata atttcaatat ctgttttttt agaaagaaat tttatttttt
    11341 ctattagttc tttttcaatt tcatcaactg aatttccaag agtaaatggt gcaatttcta
    11401 atccttcata tccaatttct tttataaaat aaaaaatttt ttctatatcc caattttcaa
    11461 acatctcatt acatatcccg attttcataa acttccccct ttttttctta aaattgcagg
    11521 tgtatcaata tttgttgctg gtttttctat ctccccaagt ggtaatttat cttcttcttt
    11581 tggtttttta atttccacct ttttcccaat tccagtggca aggagggtaa tttttacaga
    11641 gttttttaaa ttttcatcaa ttgcaactcc aagaacacga aatggagaga atatttttga
    11701 ctgtattagt ttcattgttt ctccaacttc aattaatgtt aaatcactgc ctccagtcat
    11761 acttataaga attttttttg cttccattat ttcgcttttt tctacaagag ggttttcaag
    11821 tatttgtttt gtactttctt ccatcctttt ttccccttct ccataaccaa ttccaactat
    11881 tccttttcct ccattttgaa caacagagca tatatctgca aaatcaatat caagtaattt
    11941 tggtttattt ataaggtctg aaatagaaga tattgttctt tcaataattt catctatttt
    12001 tccaaaagca gaagcaaagg aaacagtatt atcaacaatt tcatagagtt tttgatttga
    12061 aatgtgaatt agtgtatcta cattatttaa aagtacctct atcccattaa ttgcatgctc
    12121 catccttttt tccccttcaa attcaaaagg agttgtaaca agggcgataa caatacttcc
    12181 aaggtctttt gctatttttg ctataacagg tgaacttcct gtacccgtcc caccaccaag
    12241 tcctgctact aaaaaggtaa tatttgtgtt tttaaaaatt tcttctattt tttctctatc
    12301 ttcatttgct gcaaatttac ctttttcagg gtctttgcct gctccaatgc cctgggttgt
    12361 tttttctcca atttgaattt taatatcccc ctttgcttgt tttaaagcat tactttccgt
    12421 attaaaaaga acaaaggaaa caccttcaat tttgtcatga attcttgaaa tgatatttcc
    12481 gccagcattt ccaactccaa ctacttttat atttattatt ccttcttctg gtgtttgttg
    12541 tatcatttga atcattttat tttcctcaac ccctttttaa agtttttttt tattttcgca
    12601 aatgtctcct ttaatttatc ataaaatgat ttctgccttt ctttaagttg tttagatgca
    12661 agagtgataa gtccaaaggc agatgaatat tctggttttt gaaaattttt atctaggttg
    12721 tatgttctta taggaactcc aattctaacg ggaagtttaa atatatctga agataattct
    12781 ataattcctt ttagtttaga gcatcctcct gttataatta ctcctccagc caatgaaact
    12841 gaaagagaat tttttacaac aactggcttt acataatcat aaagaatttc agtaactctt
    12901 gcataaacaa tctccgaaat tttttcaact gtaacttttc ctattatttt accagatgga
    12961 ctggatattt caatctcttc aagaagttta gggtccttat tttctttcat tttcagataa
    13021 ttacaatatc catagttaat ttttaattct tcagccagct caatgggagt gtatagaaca
    13081 gcggataggt cattatcaat ataccagccc ccaattctaa aagatgccac ttcggttata
    13141 gtgtttttga aatagatagc aatatctgtt gtccctttgc ccatatcaat taaaagacat
    13201 cccatttctt tttcttcttc tgttaaggtt gcttctccgg atgcccatgc atgtgggaaa
    13261 atatgttcaa tctgtcctcc tgcgctttca atacaatgtc caatatcttt aacaggattt
    13321 atttcaacag atattgcaag aactttcatt tcaagagtat ttccatgcat tccaatgggt
    13381 aattttgtag ttatattatg tccatctatt atgtagaatt gaggaagaat tgttaatatt
    13441 ttttcaccaa cttcttttcc tgccataata gaatttttta tttctttttt taaaaattga
    13501 atatcattct tatttatttc ccttccatta ggagttattt ctattttttt tgaatatata
    13561 cttcctttta cataaccgcc acctattcca attgttacat aacttattct ttctttaacc
    13621 tgctcactta aagaatcgaa aacatcaaaa agtaaatttg ttactccttc aatatcagtt
    13681 atcctacctt ttgttatgaa attttcaggt acttcaacga tttctgttcc aagaatgtct
    13741 attcctgtat ctgataaata acctgtaatt cctaaaattt ttccactgcc aatatcaagc
    13801 cctgtaatta aattttcatt cattttttgt aaatattttt atatctcaag tcaatatatt
    13861 cccatctttc tatatttccc tgcaattctt gtaataggat tgaaattttt tccatctgtg
    13921 actttatatc actcttcttc atttttataa ttcctttatc agtatataaa attatttcat
    13981 ttggagaaat aaaatttatc tggttaacta aaaaaaattt tcttaaattg aagtaattat
    14041 accatttttt aatttcatcc aaaatattta atttccatct atcagtttca tttacacaat
    14101 tttgctcaat tttaattcca tatatatcaa tataattttt ttcttcttct atatctgata
    14161 gaacaactcc atttttatca attatatatt ttctctcatt gtatattaca ctgacaaatg
    14221 gttttcttaa tgaaatatct atatataatt tatcaggata tattttttta attttgcagt
    14281 tttcaacttc tggaatatta agtatcattt gtcttaaatc atcaatatct ataaaaagta
    14341 gatttttatc tttttcaagt tctaacactg gctcaatata aaattttgcc ttatcaggaa
    14401 caatttcaac ttcttttaat ttgaaggtgt ccaattgcca taaaaatttt attgttgaac
    14461 ttttaatttt aaaagataaa tatattaaag ataaaattaa aataatgata aaaattattt
    14521 ttattttatt ttttcttttt tcccagattc tttgtcttat ttctcttatt cttctttctg
    14581 ttttttcttc catttttcaa ttgcataatt taaaatttgt tcacataaat catcaaaatt
    14641 tatccctgca tattttgcag catcaggcaa taaacttgtt ttagtcattc caggaattgt
    14701 atttacatct aaaatatatg gtacaccatt tttattaatc attatttcca ttcttgcaaa
    14761 tccactgcat ctcattatct tataaacttt ttttgcattg ttataagcat ttttaagaaa
    14821 tttttctgga atttcaggag gaataatgtg ttgactttca ccaggagtgt acttcgcttt
    14881 ataatcataa aatcctgttt ttgttcttat ttcaatcgca ggtaataccc ttattttttc
    14941 attcccaagt attccaatgg ttatttcttt cccttcaata aatttttcaa taaagacatc
    15001 attatctaaa ttaaaacatt ctttcactgc tttttttaat tctttttctt tttttactat
    15061 tcttattcct atggttgaac ctaaatttgc tggttttact acaacaggaa aagaaaaagg
    15121 aagatggaat tttttatttt tttcaacgac tacaaaaggt ggggtaggta ttttatgaaa
    15181 tttaaggaat ttttttgtta taattttatt catacacata gcagaagaaa gcactcctga
    15241 accagtatag ggaatattca gtgtttcaag agttccctga ataactccat tttctccttt
    15301 tccaccgtga agaacaataa aaacacagtc tggcttaaat tcaataagtt tttttaaaaa
    15361 agtttttttt gatgggtcaa gcaaaataac actataattc tttcttttta aagcattata
    15421 tacttgtttc cctgacatta aagaaacatc tctttcggga cttttccccc cagcaagaac
    15481 aattattttc ataactttta ttttaataga ttggtttttt atgtcaattt taaattgata
    15541 aaagaatatg tgtgtgttga cagaaaaatt ttttgtagta taatttacga tataatagta
    15601 tcaatacgac aatgtagaaa atggaaaaga aaataaaagt aatagaaaaa ataatgggaa
    15661 tatttgaagt attagcagat aggggaaaag agggttgtag tgttagtgaa atcgcaaaga
    15721 aattaaactt gaaattaacg acaactcatt cacttgtttc aactctattt tcacttggtt
    15781 atcttgaaaa aaatattcag acaagaaaat ataaaattgg agaaaaacta ttgaatatat
    15841 ttttaccttt tgctaatagg aatattttac ttaaagtttc agagccagtt atgaaagcac
    15901 ttgcagaaga aataaaagaa tcggttgtac ttgcaatttt ctggaaaggt gaaagatata
    15961 caatagcaac tgcatcttat gacaaacaat taatatcagt taatttaaac caatttatat
    16021 atagttcttg ttacagccag gcaacaggta gagttttact ttcctattta gataaaaagg
    16081 tggttaatga gtatataaaa agaaatggtt ttccaaaaga aaaatggaat aatataagaa
    16141 attataatga cttacaaaaa gaacttaaaa aaataaaaga aaagaaaatt gaaataatag
    16201 agagagaaac agttgtagca atagcagttc ccatttttag ccctgatgga aaagtatgtg
    16261 cttctcttgg tgtttattta ccaaggataa gatttaaagg acaaagaaaa gaaaaaataa
    16321 taaaagaaat aaaaaaagcg ggcgaacaaa ttaccttaaa cttaagagga gaaaaatgat
    16381 aaaagcagag aatggcagga tagaatttaa agaaaatctt aatgtgaaat atgaagtgga
    16441 tgttgttgta gttggtggtg gaacagcagg atttgcagcg gcaataagca gtgcagagaa
    16501 tgggatgaag acgatgctta ttgaaaaaca gggagcactc ggtgggctgg tgacccttgg
    16561 tttggtatgt tacatggcaa gttatccaga aggaatagga aaaagattac tggaaaggtt
    16621 agaagatgga aagggtatag cagaaaggat ttgtgatcct gaaaaaacaa agtatattat
    16681 ggaacaaatg gcgattgagg caggagtaaa aattctttac tggacttatg ttattgattc
    16741 aatagttgaa ggaaatgaaa taaaaggagt tgtaatacaa aatatttccg gtagaagtgc
    16801 aattttagca aaaagagtta ttgattgtag tggagatgca gaagtttcct atttttcagg
    16861 agtacctgtt gaagaaggat gggaattaat ggatggatat aatcaggcag tttctttaga
    16921 ttttgtttta aataatgctg atacaacaaa atttcaaccg agggattttt attctacaat
    16981 tcagatgaaa atagaagaag cagcaagaaa aggaaaatta ccacgacttg ttgaaaaagg
    17041 atatttaggt ccatttccag gaagaccatc agatagagga gaagtttatg cttgtactgc
    17101 tcatagcaga aaatgtaaag caacagatgc agaggattta acaagaattg caattgaaca
    17161 gagaaagcaa attcaacaat tagtagaatt ttatagagaa actgtttgtg gatttgagaa
    17221 ttcatggtta agttatactg caactatttt aggagtaaga gagtcaagaa ggattgttgg
    17281 agaatataaa tttacaggag aagaccttgc attagcaaaa aaatttcctg atgcaattgc
    17341 aagagacaca catggttttg atatacataa tcccataaat gacctgcctc atataaaaca
    17401 tactcatttt aaggaaccaa aagaaccagc attttgtact aaagaaccaa gacaagacgg
    17461 aagggtttgt tgtgtgaaaa ataatccaaa aggggaatat agagcatatt tgaagccagg
    17521 gcaatattat gaaattcctt atagatgcct tgttccagtt aaaattgaca atttacttgt
    17581 agcaggtaga tgtatttctg ctgattttga aggaagtgct ggaacaagat taataatgac
    17641 atgtataaca ataggacatg ctgcgggaac tgctgcatct ctttcaatta aagaaaatgt
    17701 tgctccaaga aaattaaata ctaatctcct aagagataaa cttgacgatg aaggtgtaaa
    17761 tttgaaaaaa gaaccaccag tttatgtaaa aggtggacca agaaaaccta ttcctgaaaa
    17821 tgctgaattt tatgtttata ctggcgatgg tgaagatgaa ataagaataa aggaataaaa
    17881 aaagttagga taaacatagg agaaaaaatg ataacgagtg aagaagtgaa agaagtaggg
    17941 aagaggtgtg gagcagacct tgtaggtatt gcatcaatgg atagatttga aggagcacca
    18001 aaacagatgg acccaagata cataaatcct gatgcaaagg cattaattgt aatagcaaca
    18061 agaatcccaa gagggacatt aaggggaatt gaagaaggga catattttct ttcatattct
    18121 tcaatgggat atggtggaat taactcagtt tacaatccaa tgattttatg gcaattagtt
    18181 agatatatag aaaatgaggg atatgaagca actcctattc caaatgatta ttataactgg
    18241 ccagcaattg attatcattc aggggtaata aaagagaact ggagtagacc tgtttcccca
    18301 gaaaaaccgg caccagatgt atttatccat ttcagaattg ccgcattttg tgctggatta
    18361 ggagagattg gttatagtaa ggtctttctt actcctgaat ttggaccaag acagagattt
    18421 gcatgtattt taacagatgc tccactccaa ccagacccaa tatatgaagg acccaaaata
    18481 tgtgataggt gtatgttatg tgtaaaagaa tgcagtggaa aagcaataag ttcaaaagag
    18541 gtagttaaaa ttaaagttgc aggtagacaa atagaatggg ggaaattaga tattgacgcg
    18601 tgttcaaaag gatttcaagg tgggaacaag aaatttaatc cttttatagg agaaccaagt
    18661 gaagttatat cttattcaag agcacttgaa ggagggaaag gatgtatccg tgcatgtatg
    18721 atacatttag aacaagaggg aaaattaaaa aacaaatttg aagaaccatt tagaaaaaga
    18781 aaaccatggg aacttaaata atttaattat tctaaaatga taaactaaaa ggaggaaaga
    18841 gaaatgataa caagtgaaga agtaaaagaa gtaggtaaga gatgcggagc agaccttgta
    18901 gggattgcat caatggatag gtttgaagga gcccctaaac agatggaccc aagatacata
    18961 aatcctgatg caaaagcatt aatagtgttt gggtttcgta ttcatagagg gcttttaagg
    19021 ggaattgaag aagggacata ttttagtggt tatacatcaa tgggatatgg tgggataaat
    19081 actgtatttg ctcctttggt attaaggcag gtatgtaatt ttattgaaga taatggatat
    19141 gaatccacag catatcccaa tcctcttgta gcagttgctg ttgatattgc aacaggagaa
    19201 tttaaaaaag gatggagtag acctgtttcc ccagaaaaac cggcaccaga tgtatttatt
    19261 cattttagga tcgctgcatt ttgtgctgga ttaggagaga ttggatatag caaggtcttt
    19321 ctcactcctg aatttggacc aagacagaga tttgcatgta ttttaacaga tgctccacnn
    19381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnag aggtagttaa aattaaagtt
    19501 gcaggtagac aaatagaatg ggggaaatta gatgtaataa aatgttctaa tgcttacaca
    19561 ggagcagtca aggaaacaaa tccattttta cctgaggatt tacctgataa atttgaagaa
    19621 tggagcaaat attatgtaga tgctaaccca cataaaaacg aatttgttaa atggtatgga
    19681 ggaataggtg gacataatcc gggacttgaa ggaggacgtg gttgtttaag ggcatgtatg
    19741 atacatttag aacaagaggg aaaattaaaa aacaaatttg aagaaccatt tagaaaaaga
    19801 aaaccatggg aacttaaata atttaattat tctaaaatga taaactaaaa ggaggaaaga
    19861 gaaatgataa caagtgaaga agtaaaagaa gtaggaaaga ggtgtggagc agaccttgta
    19921 gggattgggt caatggatag gtttgaagga gcccctaaac agatggaccc tcgatatata
    19981 atgcctgagg caaaatcaat tattgggatt ggttttcgta ttcatagagg gttatttcgt
    20041 ggtattgaag aagggacctt ttttgcggca tatactgcaa tgggatatgc gactattaat
    20101 gatggatatg cgccaaaagt actcagagaa gtaggaaatt ttattgaaga taatggttat
    20161 gagacgatgc tctatcagaa tacagtgttc ggttaggtat tggtgtagga gaacctgtag
    20221 cacctgataa accacaacct gatgttttta ttcactttcg tatagcagca tttatctgtg
    20281 gaatgggaga gattggttat agtaaaatat ttcttacacc agagtttgga ccaagacaga
    20341 gatttgcatt tattattaca gatgcaccac tccaaccaga cccaatatat gaagagccaa
    20401 agatatgtga tagatgtatg ttatgtgtaa aagaatgtcc ttcaggtgct attagtagaa
    20461 aagaaacagt agaagtaaaa gttgcagaaa aaacacttga atggggaagt ttagatgaac
    20521 tcaaatgtgc tgcatgctat caggcaggta cacctgaata tagtccattt atgcctgaaa
    20581 aagtagccaa aacattagaa ggaattttca aatatcaatc tggttcggag tcaaaaggaa
    20641 tgactgatta tactggagat gtatgggcat atctcaggga agaatttcca tacattcgta
    20701 atgcatggga aagttatcat caccctgcta atatatgtgg agcaaaaggg tgtattcgtg
    20761 cttgtatgat acatttagaa caagagggaa aattaaaaaa caaatttaaa caggagttca
    20821 ggaaacatca accatgggta ataaaacgga aataaaattt tcagttgggt atcaattaag
    20881 agaagataaa ctttttaaag atattgttga gagatataaa gaaaaaattg aagaagttta
    20941 tttttcttgg ttaaatttgc caagtggtag aggtcccatt gcagaaaaag tcggttatat
    21001 aaattgggaa ggacaaaaac aacttgaata tgaactaaat tgtattaaaa aaatgggaat
    21061 aaaattagga cttcttttaa atgcttcatg ttatggtgga tttaatcttt ctgtttcact
    21121 tgcaaataca gttgtatcaa caatagagta tcttcaagag aatattggga tagatatagt
    21181 tacagttttt tcccctgttg ttgcctatat tattaaaaaa aattttccag ggataaaaat
    21241 aagaggttca gttaatttaa gaattgggac agttgaggga atggaatatg tatctgactt
    21301 atttgatagt tttgttatgc aaagggaata taatagggat tttgaaacaa tagaagaact
    21361 taaccagtgg tgtaataaaa atgggaagac attatgtatt cttgtaaata gtgggtgtat
    21421 gaatttttgt gcagttcagt tgtttcatga caataccata tctcatgaaa tagatgttta
    21481 tacaagaggc aatataacag aagaaattcc aggtaattgc tggtcatatt ataaaaagag
    21541 agaaaattgg agaaaattat tgcaaaactc atcatggata aggccagagg atattcataa
    21601 ctataaagaa aaattttctc atttcaaatt agcaacaaga ataaatcagg agccagaaaa
    21661 aattataaaa gcatactgta atgaaaaatt tgatgggaat ttacttgacc tttttgagcc
    21721 atcacacacc aaacttttgt ttccttatat tattgaaaat acaaaattcc cggaagattg
    21781 gtttcagaaa ataagtagtt gtaaaagaaa ctgtgataaa tgttcttatt gtaaaaatat
    21841 attagaaaat attcttgtaa aattttaaaa aggagaaaaa atggatgaga aaaaaaagtt
    21901 gacagaagaa gtaaagagat atgcaaagag tttaggagtt gatatggttg gaattgcacc
    21961 tgttgaaaga tttaaaaatg cccctttgag gatgagtcca aaagggcttt tacctgaaag
    22021 taaaagtgta attgttttag gtattcatca tcttgattgt gcagttgaat taggtggtta
    22081 tccaacacca cacgatatag gaccttatgg cacacagagt tctgttatga atccaatgct
    22141 tgatgatttt gcctttaagg tagcaagatt tcttgaagat aagggatata aaacacttcc
    22201 tatacctgta actaatatat ggagatacaa cccatataag gatttagatg tctcatttgc
    22261 tcctgacctt gctcacagat atgcagcagt tgcagcagga cttggtgaaa ttggctggag
    22321 tggtcttttt ttatcatctg aatttggtcc aagacaaaga ataatatcaa ttataacaga
    22381 agtagaactc ataccagacc caatgtatga aggtgaaaaa ttatgtgata aatgtatgga
    22441 atgtgtgaaa cattgtccaa cagatgcatt tagaaaagaa gtcaaaaaaa taaataaaat
    22501 agaaattggg aataaaattt ttgaatttcc tgatacaaat aagtggagat gtgcatgggc
    22561 tgaaaatttt gctctaaacc tgtctctccc catacctgat aaagttgatg aaaaagtaat
    22621 attggaaaat ttagaaaaat atggaagaag aggaggagaa gaaggttgtt gccttaaata
    22681 ttgtatggta aaggaaaaaa gatattatca attgaactat acagatgcac caagaagaaa
    22741 aaaagaaatt acaaaaaaag cagatgtaat aaaagaagaa attttaaaaa tggtacagga
    22801 tgaatttgtt gatgttattg ctattgcaac tgtagaaaaa tttgaagatt cgcatttttt
    22861 ccatcctgaa tttcatcttc ctgatgcaca gacagttatt tcaataggaa taaaaattga
    22921 aaatgaaaca ttagaaataa gttctgctgt tagaagaaca attgattttc tttcatataa
    22981 aatatctcat cttcttgatg tttctggtta ttcatcaatt acatctggga atgttcccca
    23041 tctaacaatt gggcaaaaat tgactggaaa acttaaaaat tactattttt caacaatttt
    23101 aacttctgca aaaatttcag aattcaactg ggaaaaaaga acaaataaaa agaaattaaa
    23161 aaaagaagat ataaaaaatt tttgtttgga gcagaaagtt gatattgtta gattttttac
    23221 aacatctcgg tatgaaaatt ttatgaaaaa gattgaaagt tatttctctg atatctacta
    23281 tgaaatagaa gataaaggag gtattcaaag tgcatttgtt cctgaaataa aagagaagaa
    23341 aatagagata aaaaaagtta gtgattacct tgaggaaggt aagtcagtta tagcaatagg
    23401 actgcatttc ccagatgcgt gtttagatac cgcaaaaata acaccagcag aaacggttgg
    23461 cccttatgct tatgctcaat atgaaacaat ttttcttttg agggatatag gattgaatgt
    23521 tgttaaattt ctacaaaaca atggatataa agcaacttta aaagaaagta taactggttt
    23581 ttattctttt gtgaaaaatt caagagggta tttacctgat atgagaacgg gaaatattga
    23641 ggcgtttctt tcaggtgctg gatatattgg agaaaatgga gtaataatta cagaaaaatt
    23701 tggacagaga cagagattta tttttattat tacagatgcc gactttgaaa atgaccctct
    23761 ttataacgga gaaaatttct gtaaaaaatg ttcccatccc tgtattactt cttgtcctac
    23821 aaaagcaata aaaaagggaa atacaatatt aaaatttgaa gggagggaat ttaaattgcc
    23881 tgatttagac atttacagat gcgattgggc aaaaaggtat ggattaagtg gaaaagaagg
    23941 tccttctttt tatggaattg aaccaaatgt ttcaatacct acaaaaatta caccagaaaa
    24001 aattgcagaa tgtctgataa atacaaaatg gggagtccag aaattacata taaatatatg
    24061 tgaagaatgt ataagaaatt gttcatataa aggagaaaaa aatggtaaat aaaaaagaat
    24121 tgaaagaata tgcagtagaa gtaaaagcag atttaattgg aattacaagt atggatagat
    24181 ttaaaggtat agataaacaa catcatcctc tttctatatt tccagaaggt aaaagtgtaa
    24241 ttatagttgg aaaaagaatt acaagaggtg caatcagagg aattgaagaa gggacacagt
    24301 tttcaatcta ttcacaatat ggttgtagtt ggcttaatga taggtttctt gctcttacaa
    24361 cttttaaaat ttcagagttt cttgaagaca atggatatga agcaattcca attgttaatt
    24421 taccttcaga aatttctcca atgggaatac ccgttaaaaa aggtaagcca tctcctaatg
    24481 ttttaattga tattgaagat gcagcaataa gaggtggact tggagaaatt ggatattgtg
    24541 gaatattttt aacagaagaa tttggcccaa gacaaaggtt tcaagtaata attactgacc
    24601 ttgaaataga accagaccca ataaaaaaag aaaatgtttg tgacctgtgc aaagaatgta
    24661 tgaaaatatg tccattaaga gcaattgaaa atgaagaaaa agaaattgag atatgtggaa
    24721 aaaaaatgat agtagcagat ataaattatg aaatctgtaa aatatgtaaa aatggtgttt
    24781 ttccgaatat gtattatgaa aatgcaaaac cagatagaat tgctgcttta tgttcaagaa
    24841 catgtttagt tcatttagaa aaaacaaaaa gaataaaaaa tttatttaat gaaaatttta
    24901 gaaataggac tacatggaaa attgataaga caggtgaaat tatgcttgat gaagggaggg
    24961 atatagaatg aaaaaattaa caaaagagac aataagagaa atgggatata aattagggct
    25021 ggatctggtt ggatttgcaa atattgaaag atttaaagat gctccagaaa atatgcatcc
    25081 caaaggtatt ttcccagaag caaaaactgt catagttgtt ggaaatagaa acttaagagg
    25141 agcatggcgc ggaatagaag aaggaacaaa ctgggtatct tatacttatt ttgattattc
    25201 tggtctggca aatacttttt ttattcagga accactttat caacttgcat gttttattga
    25261 ggactctgga tatgaagcag ttccttacta ttctggagtt ccagaagcac aatcgccttc
    25321 tttaaaacca ctaagagaga atcaaccatc tcctgatgtt catttagcaa taagaattgc
    25381 aggaatttgt gcaggacttg gagaaattgg atggagtaag gtatttctga caaaaaaata
    25441 tggtccaaga caaagattgc atgcaataat tactgacctt gaagttgaac cagaccctct
    25501 tgtaaaaccg ttaactatat gtaaaagatg catgaaatgt gtatctgact gtccttctca
    25561 tgcaatacca catataaagg aaaataagaa aataagtata aacatagaga attatactta
    25621 tgaatgggca gatatagaca tgggaaaatg cactttgagt tatcatggtg gtgattcaag
    25681 agtttctcct tttattcata aggactttcc tggttggcat attgacgcaa gaaaacaaca
    25741 attttcagaa gaatgtgcat ataagttttg ttggacatta tcaacaggaa gatggagaaa
    25801 aacaaaagaa ttcccatctg gttatataat tgaaggacat ggatatttac agaaatgggg
    25861 agtaggtgga agttatggta tttgtggttc aagaagttgt atgagaagtt gttttaatta
    25921 tcttgaagag aaaaatttta tagaacagaa atttcataat ggttcattta taaagagaaa
    25981 aagatggctt cttccttatt cagttaaaaa ataattt
//
LOCUS       DAOOVW010000023        25654 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_1291_length_25654_cov_9.771163, whole genome shotgun sequence.
ACCESSION   DAOOVW010000023 DAOOVW010000000
VERSION     DAOOVW010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 25654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25654
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1291_length_25654_cov_9.771163"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..471)
                     /locus_tag="PLW95_04490"
     CDS             complement(10..471)
                     /locus_tag="PLW95_04490"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOV21921.1"
                     /translation="MKEEIKERLINYLKFLESEWEDYHEYENFTWDIYYKNRKKRREI
                     ERWIENVLNSVIDISKVIIISEEYKLPETYKEMVRFLSLFKHFEKRCMDGLSSYVNLR
                     NIITHEYIDIKWNSIENFLKDSKSLIPIFINSVQKYLKEKLKKGNGDSVKK"
     gene            complement(471..899)
                     /locus_tag="PLW95_04495"
     CDS             complement(471..899)
                     /locus_tag="PLW95_04495"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOV21922.1"
                     /translation="MEKISVDPSLKEIIKKLNSYFKTKKEVAFVFLFGSFVHNNIHKN
                     SDVDIGIYFYPQKNNIEVESSTKYKTENEIWLDVERILKREVDLIVLNRASSKICFSA
                     LRGIPVIIKDWKLYLKFMAIVSDEGINYQQMVISEFKNTL"
     gene            complement(924..1121)
                     /locus_tag="PLW95_04500"
     CDS             complement(924..1121)
                     /locus_tag="PLW95_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21923.1"
                     /translation="MKAMVEIEKIVDLLNSLDKKTKQKILEKMLISYDESPLSLEEKN
                     LIKEANDDYKKGNVIRCKIGK"
     gene            complement(1275..2582)
                     /locus_tag="PLW95_04505"
     CDS             complement(1275..2582)
                     /locus_tag="PLW95_04505"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HOV21924.1"
                     /translation="MRNKILKVMAVIFAVALISPSFAAVESVKVGGDITMYGVLRPNF
                     NVGPANEKSQNFFQTSIRVYVTADLTDNVQAMIRLIDEMDWGSDLVGNDTRDILLDLG
                     YIKVTDLMIPGLTLTVGRQEIQFGEGLVVGSAYNGYTYANDSPIAARDLGLQKAFDAI
                     RADYEVATLPITLTVFGAKITEDQTSLFGRVPTGDTGDRNLYGLNIGWRPDICALDVY
                     YVRDDTLHTDQNVDTAGLRVTGEIPAVENLGLKLEYAKQFGEDAAGDDNKGWALLFGA
                     DYKFPVNMNPYVKLNYNLFSGNDNDNDNENEGWRMVFPSNIGSRMGQVWYALTSDPNN
                     RGRGIRESNLQAINLGFGLQPVEKVKLALDAYWLKGAEDIVGTEDDIGTEIDLGIEYQ
                     YTEDLSFGLSYGMLLTGDLFDTVQFGRDDDNPWQLLATMKLAF"
     gene            complement(3419..3919)
                     /locus_tag="PLW95_04510"
     CDS             complement(3419..3919)
                     /locus_tag="PLW95_04510"
                     /inference="COORDINATES: protein motif:HMM:NF021515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2242 domain-containing protein"
                     /protein_id="HOV21925.1"
                     /translation="MRGLDKLVSVILGIMVLGTGCATFKPSKEVFSNKDINTRIYNAS
                     TETCWLALKQTLLKNNFAIEKEENQSQIQATKSFEKKALTVNVVLQVNLQPYENDRTQ
                     IFLNATQITKKTYTSRRTTMLLLIPVPTGTDASQTQTEKTIEDEKFYNAFFDQIAAEI
                     KNLTGK"
     gene            complement(4201..5823)
                     /gene="carB"
                     /locus_tag="PLW95_04515"
     CDS             complement(4201..5823)
                     /gene="carB"
                     /locus_tag="PLW95_04515"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOV21926.1"
                     /translation="MGKRTDIKKIMLIGSGPIVIGQACEFDYSGSQACKALREEGYEI
                     VLVNSNPATIMTDPEMAHKIYLEPLVPDILEKIIERERPDALLPTIGGQTGLNLSTTL
                     YETGVLQKFGVELIGANYDAIKKAEDREYFKNAMKKIGLKIPESGIAHTVEEGIEITK
                     KIGFPVIIRPSYTLGGAGGSVAYNIDEFEYLGENGIKSSLIGEILVEQSVLGWKEYEL
                     EVMRDKNDNVIVICSIENFDPMGIHTGDSITVAPVQTLTDKEYQVMKNAGIDIIREIG
                     VEGGGANIQFAVNPENGEMVVIEMNPRVSRSSALASKATGFPIAKITAKLAVGYLLEE
                     IPNDITKKTPSCFEPSIDYCVVKIPKFTFEKFKGAEDILTTSMKSVGEVMSIGRTFKE
                     ALQKALRSLEEKYFGLEDINLIGELEEGLKKPNSKRLFYIKEAIKRNWTLDKIYQLTK
                     IDKWFLYNMSEIISVEEEIKKTKGKISEKLMKKAKEYGFSDRQIGKLTGRDEREIRDI
                     RENYKIVVDYKLVDTCAGEFEAETPYYYSTYE"
     gene            complement(6531..7598)
                     /gene="carA"
                     /locus_tag="PLW95_04520"
     CDS             complement(6531..7598)
                     /gene="carA"
                     /locus_tag="PLW95_04520"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOV21927.1"
                     /translation="MKGYLVIEDGSIYEGECFGVEGESFGELVFNTSMTGYQEIIYDP
                     SYKGQIVVMTYPLIGNYGVNSHDSESYKTHINGFVVKERSKIYSNWRAEKSLEELFVE
                     NKLIGIEGIDTREVTIKLRDKGNLKSGIFTGKRDKEEMLEKVKISPSIIGRDLVKDVY
                     QNEPYWWNEEGKYKVIVIDCGVKYNTLRLLNNLDIKVKVVPANYPYEEIEKEKPDGIV
                     ISNGPGDPSVLAYVINTVSNIVEKYPVFGICLGHQILAHVFGGKTYKLKFGHHGGNHP
                     VKDLKTGKVLITVQNHNFCVDINTIKGQDIEITHINLNDGTLEGIKHKKLPVFSVQFH
                     PESAPGPNDAGYIFKKFLELL"
     gene            complement(8044..9423)
                     /gene="purF"
                     /locus_tag="PLW95_04525"
     CDS             complement(8044..9423)
                     /gene="purF"
                     /locus_tag="PLW95_04525"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOV21928.1"
                     /translation="MKEDCGIVGIYSNVKDAYQTLYFSLFALQHRGQESCGIVISDGK
                     NIYQKKGMGLVYDVLLSENSNELKGYYGIGHVRYSTTGSSKVENTQPFVADYKGKTYA
                     IAHNGNLTNSYQLRKKLEENGTIFQTTMDTEIIMHLIVKSKKDTFEGKLIDAVLKLEG
                     AFSLVIFTPTCIYAVKDPHGIRPLSLGKINDGYVVASESCAFDLIEAKYIREIEGGEI
                     IKIDKEGVKSINYGKKKDARCIFEFIYFARPDSMVFGESVYKVRKNFGMKLAEEFPFD
                     GDIVIPIPDSGNIAAIGFSEKKKIPFEMGMIRNHYIGRTFIQPFQTTREMQVKIKLNP
                     VKEIIRGKKVIVIEDSIVRGTTSKFRIKSLRKAGAKKIYMGVSCPPIKFPCFYGIDFT
                     TREELAASKKEIEEIRKSIGLDGLYYISLEGMLSSSSISPEGFCTACFTGKYPIKVKN
                     FKKEQFETE"
     gene            complement(9425..10126)
                     /locus_tag="PLW95_04530"
     CDS             complement(9425..10126)
                     /locus_tag="PLW95_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HOV21929.1"
                     /translation="MSTGITKKEIKAIYEISKAITSDMYLEDILKLIVSVTAEITGSK
                     ICSIMLVDEKTNTIKIRATQSMSNEYIKKEPLKIGEGIAGKVVKEGVPIYIEDVRESS
                     EYKYRDIAKTEKLISMLSVPMKVKNKVIGVLNIYTPVVHKFSEKEVEIISSIANQAAI
                     VIENTELMVKTKILEEELETRKKVEKAKGILMKELGIDENEAFEKLRKASMDKRKSLR
                     EIAESIIIASEIRGK"
     gene            complement(10193..11110)
                     /locus_tag="PLW95_04535"
     CDS             complement(10193..11110)
                     /locus_tag="PLW95_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458994.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOV21930.1"
                     /translation="MKNTERYYKFSKYLKEKFGSNVFKISVDAGFTCPNLDGTLSKNG
                     CIYCDNYSFSPSLRKKGISLEEQIKNGIEYGEKRYKAKKFIVYFQPFTNTYAPVNVLK
                     EKYDVIKKFENIVGLSIGTRPDFIDEEKLKLIESYTEKYDVWIEYGLQTIHNKTLKII
                     NRNHTYEDFLQGIEMTKKRNIKICCHVIIGLPGETKEDIIETAKKCGQLQIDGIKIHP
                     LYIVKGTELETLYKEGKYKPMERDWYIEVLIEFLRYLSPETVIQRITADCPNELLVAP
                     LWLKNKQKFLKMIDEEMERRDIFQADRYD"
     gene            complement(11085..12935)
                     /gene="mnmG"
                     /locus_tag="PLW95_04540"
     CDS             complement(11085..12935)
                     /gene="mnmG"
                     /locus_tag="PLW95_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOV21931.1"
                     /translation="MEKFDIIVIGGGHAGIEASLSSSRLGLSTALITFTKDKIGFMSC
                     NPAIGGVGKGQIVKEVDALGGQMAKTTDAVGIQFRILNKSKGPAVWSSRAQVDRDNYA
                     KYMQDIIEKEKNIKVIEGECTYLLIKENNVYGIEINNEIKIEGKVVVLTPGTFLNGLI
                     HIGLESFPAGRIEEKKVSKKLSIQLKNIGFKMLRFKTGTCARLDGNTINFDILKKQEG
                     DTPPIPFSFSTEKIEIEQLPCYITYTNEKTHKIIRENLDRSPLFTGKITGIGVRYCPS
                     IEDKIVKFPHHQSHHVFLEPEGKNTSLIYPNGISTSLPVDVQDDFIRTIPGLENVKII
                     RYGYGIEHDVVEPTCLYPTLETKLVRNLFFAGQINGTTGYEEAAGQGLIAGINASLKV
                     KGQKMITLNRTNSYIGVLIDDLTTKGTNEPYRMFTSRVEYRLLLREDNADIRLRETGY
                     KIGLVRKEEWEKTREKKEKVKEIIKKIKTKKIKVENGKITLFEYLKRAGVKLSDFDEF
                     KSYPEKVLFETEVEVKYSPYIKKQLKEIREFKNLEKIKIPEDMNYNEIPGLSLEIREK
                     LSKFKPLNLGQASRISGITPSAISLLLVYLHTRSDRPCPEKKDEKHRKIL"
     gene            complement(13512..14783)
                     /locus_tag="PLW95_04545"
     CDS             complement(13512..14783)
                     /locus_tag="PLW95_04545"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOV21932.1"
                     /translation="MKNISELQFLENLSDFGIKLGLDKIKFILEKLGSPHLKYPSILV
                     GGTNGKGSVCKCLSEITRESGYKTGLYTSPHLVDVKERIVVNGEKITDEEFIEKIKQV
                     RRIIEKLPYHMYPTYFETLTAIAFLYFYEKGIDIAVCEVGLGGRFDATNVLPSEINVI
                     TEIGLEHTKLLGKTYESIAGEKAGIIKEGSLVVVSKQRKEVEKVIKEKCKQRKAKIYF
                     YGKDFKSIKREESIDGIVFDFYGKENFKNVKTKIIGSHQIENLSLAIQSSLLLRKKGF
                     KVKKENIYSALENLHWEGRFEILKKDPFIIFDGAHNPDGVKVLIKNLKKYFPFKLKLL
                     IGILKDKDYTKMLSYVSKYNPEEIVFTTPETERALDPEILSNYMKKSNTKIKVIKDVN
                     NAMEYIKKTGDSWCIFGSLYLYSSVNVNLRS"
     gene            complement(14780..15619)
                     /gene="accD"
                     /locus_tag="PLW95_04550"
     CDS             complement(14780..15619)
                     /gene="accD"
                     /locus_tag="PLW95_04550"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HOV21933.1"
                     /translation="MFRRKKFTEISPKEKVEIKKEMWIKCDNCGKMVYKKTWEEKQKI
                     CPSCGKYSRLTAYERIRAIADENTFKELWKDMVSGDPLNFVGIKPYKEKLKEDMEKTR
                     LNEGLVVGECKIESIPSVLSVLDPHFIMGSMGAVVGEKFTKGCEYAIASGFPFICISG
                     GGGGARMYEGIISLMQMAKTSSAVGRLKEKGILYISVLTDPTMGGVAASFAFLGDIII
                     AEPGALIGFAGPRVIEQTIKQKLPPGFQTSEFLYQHGMIDMIVKRDELKETISKILKV
                     FFT"
     gene            complement(15619..16491)
                     /gene="dapA"
                     /locus_tag="PLW95_04555"
     CDS             complement(15619..16491)
                     /gene="dapA"
                     /locus_tag="PLW95_04555"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOV21934.1"
                     /translation="MFYGSIVAIVTPIKNGQIDKESYKKLVEFHLKNGTDGIVTCGCT
                     GEPATLSMEEHKQLIKFTVDIVDKKIPVIAGTGSNNTKESLELTSFAKEVGCDGCLLI
                     TPYYNKPTQKGLYLHYKTIAESVDIPIILYNVPSRTGISISPETVAELSKIKNIVGIK
                     EASGNFDQIYNIISLVDKDFVVLSGDDALTLPIIAVGGKGVVSVAANIVPKEVADMVK
                     SALNGQMKKAREIHYKLYPIFKVLFIETNPIPVKTSLSLMGMIKDEWRLPLTPPSDEN
                     FQKIKETLKKYGVI"
     gene            complement(16812..18392)
                     /locus_tag="PLW95_04560"
     CDS             complement(16812..18392)
                     /locus_tag="PLW95_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21935.1"
                     /translation="MKETFLCLDVESSFFGLYEKLRSIVANDSRVYHSKNEFIRTNPH
                     WVRDHIHEMKGYIYWQKEIRSFLDLILKNQNNKGFFYEIFAPFNSPIAGHLNFVGGDC
                     RVLFPDLGYGLVRLEVEADIEYLIVEGVHRIWQATGDTKWLEKNLNHLEKGIKYCLSD
                     PKRWDNKYKLMKRPRTIDTWDFINRPDTGINRRILPDDPMGIMHGDNSGLYAAMIQLS
                     KMFEICGQERKSSFWKERAEELRGRANITLWNGKFYIHHLPLDNAFYGVDEREQLSLS
                     NAYDINRGLPTHKMAVSILDEYQKLRDLLKGEYFAEWFSLYPPYPQFYTYSAGSYING
                     GIASFVAGELAHAAFQHGREEYAVDILKRIAKKINEDGTLYFLYTKDGKNQGGGPSGW
                     GAASLIYALIEGLAGIDDQKCSYEKVKISPRWVSADEKYAHIKIVYGPSDNTLEYDFR
                     WDKEAKIIYLKLYPGPKEVEWNILLPPNYSLEKVERNKKIIPFKLANVEKSKYTVIKN
                     NVVPAKGEFLTLFLQETL"
     gene            complement(18812..19087)
                     /locus_tag="PLW95_04565"
     CDS             complement(18812..19087)
                     /locus_tag="PLW95_04565"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HOV21936.1"
                     /translation="MIEKIGDITKRIVDIWKTEDKNREIDIDVLWNRIIDDNLQGHTY
                     VNGMKNNILYIKVDSSCYLSLLNMKKKEILKKIKDNGFSIKKIVVKL"
     gene            complement(19084..20181)
                     /gene="dnaN"
                     /locus_tag="PLW95_04570"
     CDS             complement(19084..20181)
                     /gene="dnaN"
                     /locus_tag="PLW95_04570"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOV21937.1"
                     /translation="MEIIINSRELDSVLNCVSKILPSKSSMEILSGILIEGKDELSFI
                     STDLENTIKVSLKCQFKKGSILIPGKQFISLIKQIPDEEIKIEEKENEVEISNENIKY
                     RFITMNADDFPRISKISSNVSFNIPGNILKDAIDKIKFCVNPNEQKPYFRGGLLEIKE
                     NNINLIGTDGRRLSLSKAELESTPSPKIKVLLSFKLLNILTDLLTDELTEISVGKNQI
                     TFKFSNILLISQLLAGVEDFPEYDVVIPDEKKCQIAIIDKDKFLSCLKRISLFTSERY
                     NKLKITLNKGIIMVSVVSPEIGQGQEKFEIGYDGEEMNFAFFPEFLIDFLQKVNEEKM
                     VFAFTDKKAPVLMRPQLNNNFLYVTMPLKIE"
     gene            complement(20283..21578)
                     /gene="dnaA"
                     /locus_tag="PLW95_04575"
     CDS             complement(20283..21578)
                     /gene="dnaA"
                     /locus_tag="PLW95_04575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOV21938.1"
                     /translation="MEKFEVENLSQHIFNNIKEQINNPRGYDIWIKPVKIIDFDGEIL
                     HIQIPNRAFYRGFFPFLEIIKEEFSKLYGFYPKIDISYGEEEKSDRKLLEINLNPEYT
                     FENFIVGPCNRLAHAAALAVAQSPGIAYNPLFIYGKVGLGKTHLMQAIGHYIKNRSDL
                     KMAYIPAEFFVNEYIQSIKNKTTYNFRNKFRNLDFLLIDDIHFIAGKGGSQEEFFHTF
                     NFLYDNKKQMILSSDRPPKEITAIEERIISRFEWGLVVDLQPPDFETRVAILKKKAEI
                     RNIVINDDIIFLIAEKVEDNIRVLEGILNRILALSKLTNSEINEELVNEIIDGFKRKG
                     KRKITLETIMEMVCNYFNISEKEIKSGKRIKNILIPRQIAMYLARDLTTSSLNSIGEF
                     FGIKDHTTVINACKKVKKLIENDTYIKNSVENIKRGLSV"
     gene            complement(21606..25394)
                     /gene="secA"
                     /locus_tag="PLW95_04580"
     CDS             complement(21606..25394)
                     /gene="secA"
                     /locus_tag="PLW95_04580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOV21939.1"
                     /translation="MEYYEDLLEKLKILNPGTKYFINRIFNKEKYSFLNECITLLKQI
                     NEKEGEVANMTKQQMRGKTEEFRKRISDGEPVDNFIIDGFALVREAAKRTLNMRHFDV
                     QIIGGIVLYKGKIAEMSTGEGKTLVATLPLYLNALQGNGCHLVTVNDYLAKRDTQWMG
                     PIFDYLGLSVGCIISYKEGKGLSSSSAFKFDLTYLPADSRFLYLYPVERKKAYQCDIT
                     YGIGSEFGFDYLRDNMAIRKEEQVQRKLYYAIIDEVDSILIDEARTPLIISGPSEEKP
                     QLYYEIDRLVRKLVRDTDYIVDEEAQTAVLTEEGIRKCERLLSIKNLYDGGHTEIVHK
                     INQALKGHTFFKRDKEYVVKDGQVIIVDEFTGRLMPGRKWSDGLHQAIEAKEGVPIES
                     ENQTLATISFQNYFKLYEKIAGMTGTAVTEAIEFKEIYKLDVIVVPTNKPLSRTEYND
                     EIYRTEKEKFISVTNEIKQMHEIGRPVLVGTISIEKAEKLSGYLRRNNIPHNVLHGKN
                     HESEAGIIAQAGKFKAVTIATQMAGRGVDIILGGNPEIIAREETIKTIWSKKGSKDIV
                     KKSDYSDVLEDLEKGYKNKIDVTEKEYGEKLSFLKSSLNEKEKIYSDLEGKAKEIFEK
                     EIFKKTIEASFDKINTRLEKLKERYKNENSLLLSLQEKFKEKPERLKEPRETAGKAYR
                     DYILYKKSIKKKYDIVLKEDIDEERNQLVEILGNFKNTPDFIKNLTTQINNYIDSLKK
                     YSSLVEKTYPSLNLENQKKTTEKYIDLLTRFIKNTTEKKENIEQIYQQFEKEEEKLYE
                     NYLKDWEQAEKKIFTEHFPNLPSLEKEYLSILNEYEQLHKNYEEIIKKERDEYEKQRR
                     KIEDQWQKAREELEKSPEEFKEIYETLLEKYKKPWEEEHQKVVSLGGLHVVGSERYEA
                     RRIDNQLKGRAGRQGDPGSSKFYLSLEDDLLRIFGSERLMGIMGKMPEGENITHPLIT
                     KMINNAQKKVEGRNFEIRKQLVEFDNVLNEQRNVIYSIRQDFLEGRNIDNYIREFIEE
                     IINNLFDEFCSLSVKSFQWNIDGLKVAIKGIFNIDAEIPSVENISNTVVFKENLLQDI
                     KNKIFSVLEEKQKEIGPFFYEIQRLIFLQVIDNRWKAHLRGIDELREGINLRVYAQRD
                     PLLSYKHEGYQLFQQMLSAIKQEGISAIFNIKVSDEKFTMHAEKQKVSADFIHKQYEQ
                     FDTITQEGEQQAIIQGPNAQPPVMVKQTPAVSGKKIGRNDPCPCGSGKKYKHCCGKNE
                     "
BASE COUNT         7511 a         4898 c         3247 g         9998 t
ORIGIN      
        1 ctctttcatc tattttttga cactatcccc attacccttt tttagttttt cttttagata
       61 tttttgaaca gaatttataa aaattggtat taaactttta ctatctttta aaaaattttc
      121 tattgaattc cattttatat caatatattc atgggtaata atatttctta aattgacata
      181 agaagaaagg ccatccatac atcttttttc aaaatgttta aataaagata aaaatctaac
      241 catttcttta tatgtttcag gtaatttata ttcttctgat attataataa cttttgatat
      301 atcaataact gaatttaaaa cattttcaat ccatctttct atttctcttc ttttctttct
      361 atttttgtaa taaatatccc atgtaaaatt ttcatactca tgatagtcct cccattcgga
      421 ctccaaaaat tttaagtaat ttataagtcg ttctttaatt tcttctttca ttataatgta
      481 tttttaaatt cggatataac catttgctga tagttaatac cttcgtcaga tacaattgcc
      541 atgaatttca aatataactt ccagtccttt attataacag gaattcctct taaagcacta
      601 aaacatatct ttgaagatgc acgattcaaa acaataaggt caacttctct ttttaatatt
      661 ctttcaacat ccagccatat ttcattttct gttttatatt ttgttgatga ctcaacttct
      721 atattatttt tttgagggta aaagtatatt ccaatatcaa catctgaatt tttatgaata
      781 ttgttatgga caaaagagcc aaaaagaaat acaaatgcaa cttctttttt tgttttaaaa
      841 taagaattta attttttaat aatttctttt aaagatgggt ctacagatat tttctccata
      901 aatatataat acccgatatt tttttatttt ccaattttgc atcttattac atttcctttc
      961 ttataatcat cgtttgcttc ttttataagg tttttttctt caagagataa tggggattca
     1021 tcataagaaa taagcatctt ttcaagaatt ttctgttttg tttttttatc cagtgaattt
     1081 aataaatcaa caattttttc tatttcgacc attgctttca ttttattttc tcctattaat
     1141 aaattttgga ttatttataa taattatagc atattttggg atttagtaga actctgcgaa
     1201 taagttccga gaagaggggg tataaaaaaa ggggctggtc gttagaccag cccctttaaa
     1261 atcatctttt tatcttagaa agcaagtttc atagttgcaa gaagttgcca tgggttgtca
     1321 tcatctcttc caaactgtac tgtatcaaag aggtctccgg taagcaacat tccataagaa
     1381 agtccaaaag acaagtcctc tgtatattga tattcaattc ctaagtcaat ttctgtccca
     1441 atatcatctt cagtacctac tatatcttct gcacctttaa gccagtatgc atctaaagcg
     1501 agttttactt tttcaactgg ctgcagtcca aagccaagat ttattgcctg aagatttgat
     1561 tctctaattc cacgaccgcg attattggga tccgaagtta gggcatacca gacctgaccc
     1621 attcttgaac caatgttaga tgggaaaacc attctccaac cttcattttc attatcattg
     1681 tcattgtcat ttccactgaa taaattatag tttaatttaa cataaggatt catattcact
     1741 ggaaatttgt agtcggctcc aaaaagaagc gcccatcctt tattatcatc tcctgctgca
     1801 tcttctccaa attgttttgc atattcaagt ttaagcccta aattctcaac agcaggtatt
     1861 tctccagtaa ctcttaaacc agcagtatca acattttggt ctgtatggag tgtgtcatct
     1921 cttacataat aaacatcaag ggcacaaatg tcaggtctcc atccaatgtt caacccataa
     1981 aggtttctat caccagtgtc accagttgga accctcccga atagtgatgt ctggtcttca
     2041 gtaatttttg caccaaagac agttaaagta attgggagag ttgcaacttc ataatcagcc
     2101 cttatagcat caaatgcctt ttggagtcca aggtcgcgtg ctgcaatagg tgaatcattt
     2161 gcataggtat atccattgta agcacttcca acaactaatc cctctccaaa ctgaatttcc
     2221 tgtctaccaa ctgtcaatgt aagtccaggt atcataaggt cagtaacttt aatatatcca
     2281 aggtctaaaa gaatatctct cgtatcgttg cctacaaggt cagaacccca atccatttca
     2341 tcaatcagtc gtatcattgc ctggacattg tcagttaggt cagcagttac atataccctt
     2401 atacttgtct gaaagaagtt ttgtgacttt tcatttgctg gtccaacatt aaagtttggt
     2461 cttaaaaccc catacattgt tatatctcca cctactttta cgctttcaac agcagcaaaa
     2521 gaaggtgata ttaaagcaac agcgaatata accgccatta cctttaatat tttgttcctc
     2581 atttttatct ccttttgtta tctattgtca tccgcctttt agacataata agcgggtcag
     2641 ttgttctgtc tctgcttttc tttctcctct acgctttcgc cttttttcac ttttttcacc
     2701 tccttttcgg catattaaac atagagagaa aagcaaatcg ttgttagttt tattataccc
     2761 tatcttttta atttgtcaag tgtttcttta atctttcttt taaatccccc tcatccccac
     2821 cttctcccca tcggggagaa ggatgaaatc cctcttaatc tccctttaat aaagggagaa
     2881 gaaactcctt cctttgaaaa aggaaggtta ggatggattt agaaatctcc ctaaaaatcc
     2941 cctctaaccc ccctttaata aaggggagga attgggggga tttgcctcac cctaaccctc
     3001 tcccactatg ggagagggaa tgggtgagat gcctttgtgc cttgcacttc tactcgccac
     3061 tctactctct cttttcagtc aacacagttg actgaccttt gtttctatta tacctgctct
     3121 ttttatattg tcaagtcatt tattcttaat ttgtcctctc ctaatttttt tattctatga
     3181 aattctttat attgtcaagg ttcttttaca gcttattttt accttataaa aaatgtcctg
     3241 tcaataggat tttaattaaa taattttctt cttaaatatt ttattacctc aaaaagcaat
     3301 ttcacttttt aattttaaat tactacctta ttatagtgcc tcataaggaa gattttcctg
     3361 ctatatgctt aatatcaatt tcgtatgccc ttatattagt ttctctttcc gttttttctc
     3421 actttcctgt taaatttttt atttccgctg ctatctggtc aaaaaaagca ttgtaaaatt
     3481 tttcatcctc aattgttttt tctgtttgtg tttgtgaagc atctgttcct gtgggtactg
     3541 ggattaacag caacattgtt gttcttcttg aagtataagt tttttttgta atctgggttg
     3601 cattaagaaa aatctgcgtt ctgtcatttt catatggttg aagatttact tgaagaacaa
     3661 catttacagt taatgccttt ttttcaaaac tttttgttgc ctgtatctga gattgatttt
     3721 cttctttttc tatggcaaag ttatttttta gaagtgtctg ttttaatgct aaccagcatg
     3781 tctctgttga agcattatat atccttgtat taatatcttt gtttgaaaaa acttcctttg
     3841 atggtttgaa agtagcacat cccgttccca gaaccataat tccaagaata acacttacta
     3901 acttatccaa tcccctcatt tttcctcctc ttttggacaa cagtactgcc aaaattggct
     3961 aagagctttt ataatgcatt tctttgtctt ttaaggtttt tataagtatt tatatccctc
     4021 cccttttttt atatattttt ttgacactat ctccattccg tcactgccag aattttggta
     4081 cctgttcatt tccccctcac ccataccctc tcccctatgg ggagagggcg agagtgaggg
     4141 gtgttttcat ctttctttca tctatttttt gacactacct tgaataatat aatatttgta
     4201 ttattcgtaa gttgagtaat aatatggtgt ttctgcttca aattctcctg cacaggtatc
     4261 tacaagttta taatcaacca ctattttata attttctctt atgtctctga tttctctttc
     4321 atcccttcct gttagtttgc caatctgtct atctgaaaat ccatactctt ttgctttttt
     4381 catcagtttt tcagaaattt taccttttgt ttttttaatt tcttcttcaa ctgaaattat
     4441 ttcggacata ttataaagaa accacttatc aattttagta agttgataaa ttttgtctaa
     4501 tgtccagttt cttttaattg cttcttttat atagaataac ctttttgaat ttggtttttt
     4561 taatccctct tcaagttcac caattaaatt tatatcttca agtccaaaat atttttcttc
     4621 taaacttctt aatgcttttt gtaatgcttc tttaaaagtt ctacctattg acataacttc
     4681 tccaactgat ttcatagaag ttgttaaaat gtcctctgct cctttgaatt tttcaaatgt
     4741 gaattttgga atttttacga cacaatagtc aatagatggt tcaaaacagg atggggtttt
     4801 ttttgttata tcatttggta tttcttcaag taaataccca accgcaagtt tagcagtaat
     4861 ttttgcaata ggaaatccag ttgcttttga agcaagtgca gaacttcttg aaacccttgg
     4921 attcatttct attacaacca tttcaccatt ttcaggattg actgcaaatt gaatatttgc
     4981 ccccccacct tcaactccta tttctcttat aatatcaata cctgcatttt tcattacctg
     5041 atactcttta tcagtcaatg tttgaacagg agcaactgtt atactatctc ctgtatgtat
     5101 ccccataggg tcaaagttct ctattgaaca tataactata acattgtcat ttttatctct
     5161 cattacttct aattcatatt ctttccagcc aagaactgat tgttcaacta aaatttcacc
     5221 tattaaactt gatttaattc cgttttctcc taaatattca aattcatcaa tattatatgc
     5281 aacagaacca cctgctcctc caagtgtata agatggtctt attattacag gaaaaccaat
     5341 tttttttgta atttcaatgc cttcttcaac tgtatgtgca attccacttt caggtatttt
     5401 aagtccaatt tttttcattg catttttaaa atattctcta tcttctgctt tttttattgc
     5461 atcataattc gcacctataa gttctacacc aaatttttga aggacacctg tttcatagag
     5521 agttgtagaa agatttagtc ctgtttgtcc tccaattgtc ggtaataatg catctggtct
     5581 ttctctttct attatttttt caagaatatc cgggactaat ggttctaaat aaattttatg
     5641 agccatttca ggatcagtca ttattgttgc tggattacta tttacaagaa caatttcata
     5701 tccttcttct cttaatgcct tacatgcctg tgaaccagag tagtcaaatt cacatgcctg
     5761 ccctataaca atagggccag agccaataag cattatcttt tttatatcag ttctttttcc
     5821 catttaacaa ctcctattca gtaattttct tcaaaaggta aataaatctc catcaacccc
     5881 cctcatcctc cccttctccc cagaggggag aaggatgaaa tccctcttta tccctcctcc
     5941 aaaaagcgcg tcggacatgc ctttgttaaa gggggaaaat actctccctc ctttaaaaaa
     6001 ggagggtggg ggtggattta gaaatccccc ttaaaatccc cctttgttaa agggggaagt
     6061 atctctcctt cccttataaa gggaaggtag ggatggattt tattaaaatc tccccttgcc
     6121 cattccgcca caaatcttgt cggacagatg cttttctaaa gaggggaaca ttaatgtttc
     6181 aaggacccgg ttctcacatc caaaggatcc ctctgggcgt agcccaacgg gcgagccttc
     6241 ggagagaacc atgtcctttt atcttattct ttcttaaaat ccccctccaa atccctctta
     6301 atctcccttt ttcaaaggga gcaattacaa agggggaagt atcaaaatcc cctctaactc
     6361 ccgttgtgct atctgggaga cgatgacgca ccaactctgc tttgctaccc tttgcttctt
     6421 tagtcggttt gttaaagggg gaagtattaa aatcccctct aactcccctt tgtcaaaggg
     6481 gagaattaaa agggaaaaca agcaccaatt ctgtttttta ttcaatttat ttacaacaac
     6541 tccaaaaatt ttttgaaaat atatcctgca tcatttggcc ctggtgcaga ttcaggatga
     6601 aattgaacag aaaaaacagg taatttctta tgttttattc cttcaagagt cccatcattt
     6661 aaatttatat gtgtaatttc tatatcctgc ccttttattg tgttaatatc aacacaaaaa
     6721 ttatgattct gaacagttat taagacctta cctgtcttta agtcctttac aggatgatta
     6781 cctccatgat gaccaaattt tagtttatat gttttccctc caaagacatg tgctaatatc
     6841 tgatgcccta aacaaatccc aaaaacagga tatttttcaa caatatttga aacagtgtta
     6901 ataacataag caagaacaga agggtctcct ggaccatttg aaattactat cccatctggt
     6961 ttttcttttt caatttcttc gtaagggtaa tttgcaggaa caactttaac ttttatatct
     7021 aaattattta atagtcgtaa tgtattatat ttaactccac aatcaattac aataacttta
     7081 tatttccctt cttcattcca ccagtatggt tcattttgat agacatcttt taccaggtct
     7141 ctacctatta ttgaagggga aattttcact ttttcaagca tctcttcttt atctctcttt
     7201 cctgtaaaaa tgccactctt taaattccct ttatctctta attttattgt tacttctctt
     7261 gtgtcaattc cttcaattcc tataagttta ttttcaacaa aaagttcttc aagggatttt
     7321 tctgccctcc aatttgaata aattttgctt ctttctttta ctacaaaacc atttatgtgt
     7381 gttttatatg attcactatc atgggaattt acaccataat ttcctattaa aggatatgtc
     7441 ataacaacaa tttgcccttt ataagatggg tcataaatta tttcctgata accagtcata
     7501 cttgtattaa aaaccaattc cccaaatgac tcaccttcaa caccaaaaca ttctccttca
     7561 tagatactac catcttcaat tactaaatac cctttcattt tcttttttcc ttaatgcctc
     7621 ttttcaagaa cccggttctc acagagaacc aggtcctttt atcttattct ttcttaaaat
     7681 ccccctcatc ctgcaccctc gggcgtagcc ctacgggcga gccttctccc ccaaagggag
     7741 aaggatgaaa tcccccttgc cccgttgtgc ttggaaaaat ctccccctcg ggcgtagccc
     7801 tacgggcgag cctaacccct cttttataaa gaggggaatg acacaccaac tctgctcttc
     7861 aaatccctct taatctccct tttataaagg gagaaattaa aagggggaga taacgcacca
     7921 actctgcctt gctatctttt gcttccttag tcggtttgtc aaagggggaa cgattgtttc
     7981 aagaacccgg ttctcatagg gaaccaggtt cttttatctt aattttttca aaatggaaaa
     8041 aatttattct gtctcaaatt gttctttttt aaaattttta acttttattg gatattttcc
     8101 tgtaaaacat gcagtacaaa atccttctgg ggaaatagat gaagatgaaa gcatgccttc
     8161 taacgatatg taataaagtc catcaagccc gattgacttt cttatttctt caatttcttt
     8221 ttttgaagca gcaagttctt ctctcgtcgt aaaatcaatt ccatagaaac acgggaattt
     8281 tataggagga caacttaccc ccatatatat tttttttgct cctgcttttc ttaatgactt
     8341 aattctgaat ttgcttgttg tccctctaac aatagagtcc tcaataacaa ttactttttt
     8401 acctcttatt atctctttta caggattcaa ttttattttt acttgcattt ctcttgtggt
     8461 ttgaaatggc tgaataaaag ttctcccaat gtaatggttt cttatcatcc ccatttcaaa
     8521 aggaattttt ttcttttcag aaaatccaat tgcagcaata ttcccagaat caggaatagg
     8581 aataacaata tctccatcaa aaggaaattc ttctgccaat ttcatcccaa aatttttcct
     8641 tactttatac acactttccc caaataccat actatcaggt cttgcaaaat atataaactc
     8701 aaaaatacac cttgcatctt ttttctttcc ataatttata cttttaactc cttctttatc
     8761 aatttttata atttcaccac cctcaatttc tcttatatac tttgcttcaa tcaagtcaaa
     8821 agcacaactt tcagaagcaa caacataccc atcattaatt ttgccaagtg ataaaggcct
     8881 aattccatga gggtctttta ctgcataaat acaggtagga gtaaaaataa caagagaaaa
     8941 agcaccttct aattttaaaa ctgcatcaat aagtttccct tcaaatgtat ccttttttga
     9001 ttttactatt agatgcataa ttatttctgt atccatagtt gtttgaaata ttgttccatt
     9061 ttcttctaat tttttcctca attgataact atttgtcagg tttccattat gtgcaatagc
     9121 atatgtttta cctttataat cagcaacaaa tggttgtgta ttttctactt ttgaacttcc
     9181 agttgttgaa tatcggacat gcccaattcc atagtatcct ttaagttcat tggaattttc
     9241 agataacaaa acatcataaa caagtcccat cccttttttc tgataaatat tttttccatc
     9301 agatattaca attccacaac tttcctgccc tctatgttga agagcaaaaa gtgaaaaata
     9361 aagtgtctga taggcatctt ttacatttga gtaaatacca actattccac aatcttcttt
     9421 cattttattt tccccttatt tcagaagcaa ttattatact ttcagcaatt tctcttaaac
     9481 tttttctttt atccatactt gcctttctta atttttcaaa tgcctcattt tcatcaattc
     9541 ctaattcctt cattaatatc ccttttgctt tttcaacttt tttccttgtt tctaactctt
     9601 cttcaaggat ttttgttttt accattaatt ccgtgttctc tataactatc gctgcctgat
     9661 ttgcaattga acttataatc tcaacttctt tttctgaaaa tttgtgaaca acaggtgtat
     9721 atatatttaa gacacctata actttatttt ttactttcat agggactgat agcattgaaa
     9781 taagtttttc tgtttttgct atatctctat acttatattc gctactttcc ctgacatctt
     9841 caatataaat aggtacccct tctttaacaa cttttcctgc tataccttca ccaattttta
     9901 gcggttcttt ttttatatat tcattactca ttgattgtgt tgctctaatt tttattgtat
     9961 ttgtcttttc atcaacaagc ataattgaac aaatttttga accagtaatt tctgctgtaa
    10021 cagaaacaat caatttaagt atatcttcaa gatacatatc agaagttatt gctttactga
    10081 tttcatatat tgctttaatt tctttttttg taattcctgt gctcattttt attgtggaga
    10141 attacactcc cataaaattg actttatttt atatcatttt cttttttacc tgtcaatcat
    10201 atctgtctgc ctgaaaaata tctctccttt ccatttcttc atcaatcatt ttaagaaatt
    10261 tttgtttatt tttgagccac aggggagcaa caagaagttc attaggacaa tcagcagtta
    10321 tacgctgaat aactgtctca ggagataaat accttaaaaa ttcaattaaa acttcaatat
    10381 accaatccct ttccattggt ttgtatttac cttctttata taatgtttca agttcagttc
    10441 cttttacaat atatagagga tgtattttta ttccgtcaat ttgtaattgt ccacattttt
    10501 ttgccgtttc aattatatct tcttttgttt ccccgggaag gccaataatt acatgacaac
    10561 agatttttat atttcttttc tttgtcattt caataccttg taaaaagtcc tcataagtat
    10621 gatttctatt tataattttt agtgttttat tatgaattgt ctgcaaacca tattcaatcc
    10681 agacatcata tttttctgta taactttcaa ttaattttaa tttttcttca tcaataaaat
    10741 caggtcttgt tcctattgaa agaccaacaa tattttcaaa cttttttata acatcatatt
    10801 tttcttttag aacattaaca ggggcataag tatttgtaaa tggctgaaaa taaacaataa
    10861 atttttttgc tttatacctt ttttccccat attcaattcc atttttaatt tgttcttcaa
    10921 gagatattcc tttttttctt aaagagggag aaaaagaata attatcacaa taaatacacc
    10981 catttttact taaagttcca tctaaattcg ggcaggtaaa tccagcatca acacttattt
    11041 taaaaacatt acttccaaat ttttctttta agtattttga gaatttataa tatctttctg
    11101 tgtttttcat cttttttctc aggacatggt ctgtcagacc tggtgtggag atagacaagt
    11161 aaaagagaaa tagcagaggg agtaatacca gaaattctac ttgcctgccc taaatttaat
    11221 ggtttaaatt ttgacagttt ttctcttatt tcaagagaca atcctggtat ttcattataa
    11281 ttcatatctt caggtatttt gattttttct aaatttttaa attcccttat ttcttttaat
    11341 tgttttttta tgtatggtga atatttaact tcaacttctg tttcaaaaag aaccttttct
    11401 ggataacttt taaactcatc aaaatcagat aatttaacac ctgccctttt tagatattca
    11461 aaaagtgtaa ttttcccatt ttcaactttt attttttttg tctttatctt ttttattatt
    11521 tctttcacct tctctttttt ctctcttgtt ttttcccatt cctctttacg aacaagtcca
    11581 attttatatc ctgtctctct taatcttatg tctgcattat cttctcttaa aagaagacgg
    11641 tattcaacac gagaggtgaa catcctatat ggctcatttg ttccttttgt tgtaaggtca
    11701 tcaattaaga caccaatata agaatttgtt ctattcaaag taatcatttt ctgccctttt
    11761 actttcaaag atgcatttat tccagcaatt aaaccttgac ctgctgcttc ttcatatcca
    11821 gttgttccat ttatctgtcc tgcaaaaaac aaattcctaa ctaactttgt ttcaagagtt
    11881 ggatataaac aagttggttc aacaacatca tgttcaattc cataaccata tcttataatt
    11941 tttacatttt caagacccgg aattgtcctt ataaagtcat cctgaacatc cacaggcaaa
    12001 cttgttgaaa tgccattagg atatatcaaa gaagtatttt tgccttctgg ctctaaaaaa
    12061 acatgatgac tttgatgatg tggaaatttc actattttat cttcaattga tgggcaatat
    12121 ctcacaccaa tacctgtaat ttttcctgta aaaagagggg acctatcaag gttttctctt
    12181 atgattttat gtgttttttc gtttgtatag gtaatataac agggaagttg ttctatttca
    12241 attttttctg ttgaaaaaga aaaaggaata gggggggtat ctccttcctg tttttttaat
    12301 atatcaaaat ttattgtgtt cccatcaagt cgggcacaag ttcctgtttt aaatcttaac
    12361 attttaaaac caatgttttt tagttgaata gatagttttt tgcttacttt tttttcttca
    12421 attctaccag cgggaaaact ttcaagtcca atatgaataa gcccatttaa aaaagtccct
    12481 ggtgttaaaa caactacttt gccttctatt ttaatttcat tgtttatttc tattccgtag
    12541 acattattct cttttatcaa aagataggta cattcacctt caatcacttt tatatttttt
    12601 tctttttcaa ttatatcctg catatatttt gcataattat ccctatctac ctgtgccctt
    12661 gaagaccata cagcagggcc ctttgactta tttagaattc tgaactggat tccaacagca
    12721 tcagttgttt ttgccatttg tccacctaat gcatccacct cttttacaat ctgccctttt
    12781 ccaactcctc caatagcagg attacaggac ataaagccaa ttttatcttt tgtaaatgtg
    12841 attaaagcag ttgataaccc taaacgagaa gaagatagag atgcttctat tcctgcatgt
    12901 cctccaccaa taacaataat atcaaatttt tccatataat atttgccccg tttatatttc
    12961 gtctcatacc atcttttgta aagaagggtg agcctcggcc tgtgtagaac aatccccctc
    13021 taatccccgt tgtgctatct gagaaacgat gacgcaccaa ctctgctttg ctacccctcg
    13081 cttctttagt cggtttaata aaggggaaaa tacttgcccc ctcatccttt ccttctcccc
    13141 gttggggaga aggaaatatc cctctcttct tctcccctct ccccgctggg gagaggctcg
    13201 cccgtagggc tacgcccgag gggatgaggt gagggggctt taaaaatccc ccttcaatcc
    13261 ccgttgtgct tggcaaatct cccctcaccc ctcttttcta aagaggggaa atattttttg
    13321 aagaagttac cccttccttt gtaaaagcct gcccgtccat ctttccgcca aaaatcgcag
    13381 cggattcgca atgataggcg aatgtggcga attttgtggc ggaggggaga agggaaaaac
    13441 tttctgacgc taatacatta caatattact ttatattctc agacactaag ttattgattt
    13501 tcaatgactt gttaacttcg taagttaaca ttgacagaag aataaaggta aagagaacca
    13561 aatatgcacc aactgtctcc tgtttttttt atatattcca ttgcattatt aacatctttt
    13621 attactttta ttttggtatt actttttttc atataattag aaagaatttc cgggtccaat
    13681 gccctttctg tttcaggagt tgtaaaaaca atttcttctg gattatattt agaaacatat
    13741 gaaagcattt tagtatagtc cttatcttta agaatcccga taagaagttt taatttaaaa
    13801 gggaagtatt tttttaaatt ttttatcaaa accttcactc catcaggatt atgtgctcca
    13861 tcaaatataa taaatgggtc cttttttaaa atttcaaaac gaccttccca atgtaaattt
    13921 tcaagtgcag aatatatgtt ttcttttttt actttaaacc ccttttttct caaaagtagg
    13981 gatgattgta ttgcaagtga taaattctca atctgatgag aaccaattat ttttgttttt
    14041 acatttttaa aattttcttt tccataaaaa tcaaaaacaa ttccatcaat tgattcttct
    14101 ctttttattg atttgaagtc cttcccataa aaataaattt ttgcttttct ttgcttacat
    14161 ttttctttta ttactttttc aacttctttt ctttgttttg aaacgactac caatgaacct
    14221 tcttttatta ttcctgcctt ttcccctgca attgattcat atgtttttcc caataatttt
    14281 gtatgttcaa gcccaatttc tgtaattaca tttatttcag atggcagaac atttgttgca
    14341 tcaaatcttc ctccaagacc aacttcacaa acagcaatat caatcccttt ctcataaaaa
    14401 taaagaaagg caattgcagt taatgtctca aaatatgttg gatacatatg ataaggtaat
    14461 ttttcaataa ttcttcgtac ctgttttatt ttttcaataa attcctcatc agttattttt
    14521 tccccattaa ctactattct ctcttttaca tcaacaagat gaggacttgt gtatagtcct
    14581 gttttatatc cactttccct tgtaatttca gaaagacatt tacaaacact cccttttcca
    14641 tttgtaccac ctactaatat tgaaggatat tttaggtgtg ggctgcctaa tttttcaaga
    14701 ataaatttta ttttatcaag ccctaatttt atcccaaaat cagataaatt ttcaaggaat
    14761 tgaagttcag aaatgttttt catgtgaaga aaactttaag aatttttgaa attgtctctt
    14821 ttaattcatc tcttttaact atcatatcaa tcattccatg ctggtaaaga aattcagatg
    14881 tttgaaaacc gggaggaagt ttttgtttta ttgtttgttc aataaccctt ggtcctgcga
    14941 aaccaattaa tgctcctggt tcagcaataa ttatatcccc aagaaaagca aaacttgctg
    15001 ccactcctcc cattgttggg tctgtcagaa cagaaatata taaaatccct ttctctttca
    15061 atctaccaac tgctgatgat gtttttgcca tttgcatcaa tgaaattatt ccttcataca
    15121 ttcttgctcc tccaccacca cctgaaatac atataaaagg aaatccactg gcaattgcat
    15181 actcacatcc ctttgtgaat ttctctccaa caacagcccc catacttccc attataaaat
    15241 gagggtcaag aacagatagg actgatggaa tactctctat tttacattca ccaactacaa
    15301 gtccttcatt taatcttgtc ttttccatat cttctttcaa tttctcttta tatggtttaa
    15361 taccaacaaa atttaaaggg tctcctgata ccatatcttt ccataactct ttaaatgtat
    15421 tctcatctgc aattgctctt attctttcat aagcagttaa tcttgaatat ttaccgcaag
    15481 aaggacaaat tttttgcttt tcttcccatg tctttttgta caccatcttc ccgcaattat
    15541 cacatttaat ccacatctcc ttttttattt caactttttc ctttggtgaa atttcagtaa
    15601 atttttttct cctgaacatt atattactcc atatttttta agggtttctt ttattttttg
    15661 aaaattctca tcagaaggag gtgttaatgg aagacgccat tcatccttta tcattcccat
    15721 aagagataga gatgttttaa caggaattgg gtttgtttct ataaaaagga ctttgaaaat
    15781 tggataaagt ttataatgga tttctcttgc ctttttcatt tgtccattta aagcagactt
    15841 caccatatct gccacttctt ttggaacaat atttgctgca acagaaacaa ctcctttccc
    15901 tccgactgca ataattggca aggttaatgc atcatctcct gacaagacaa caaaatcctt
    15961 atcaactaag gatataatgt tataaatctg gtcaaaattt ccacttgctt cttttattcc
    16021 aacaatattt ttaatttttg aaagttctgc gactgtttct ggagatatag aaatacctgt
    16081 tcttgatggg acattgtata aaattatagg aatatcaaca ctttctgcaa ttgttttata
    16141 atgaagatat agtccctttt gagttggttt attataatat ggagttatca gaagacatcc
    16201 atcacatcca acttcttttg caaatgaagt aagttcaagt gattcttttg tattatttga
    16261 ccctgtgcct gcaattacag gaattttctt gtctacaata tctactgtaa attttataag
    16321 ttgtttatgt tcttccatag aaagagttgc tggttctcct gtacaaccac aggtaacaat
    16381 tccatctgtc ccatttttaa gatgaaattc aacaagtttc ttataacttt ccttatcaat
    16441 ctgcccgttt tttataggtg ttacaattgc aactattgaa ccataaaaca taatttcctc
    16501 ctttttaaga ttctttcaca ccatccaaac atcactcctt ataggataaa atattttaaa
    16561 aaagtagtaa aataaataaa aagataattg aaaacttttt aagttggtca ggggaaatag
    16621 gagaggacca tattctccta gttcataaaa gaaccgaagg aagaatgtcc ctcctttttt
    16681 cgctttatta tacaaaattt tcagtctcaa ccttaatatc tcccttcact cactactgaa
    16741 gtatgaacga agttgaatga atattaaagt tttactaaat attatcacaa ctttttagaa
    16801 aaaaataaga attataaggt ttcttgtaaa aataatgtaa gaaattctcc ttttgctggt
    16861 actacattat tttttattac tgtatatttg cttttctcca catttgctaa tttgaatgga
    16921 ataatttttt tatttctctc aactttttct aaggaataat taggtggtaa taaaatattc
    16981 cattcaactt cttttgggcc aggataaagt tttaaatata ttattttcgc ttccttatcc
    17041 caacggaaat catactctaa tgtattatct gatggaccat aaacaatttt gatatgagca
    17101 tatttttcat ccgcagatac ccaccgagga gaaattttta ctttttcgta agaacacttt
    17161 tggtcatcta ttcctgctaa tccttcaatc aaagcataaa ttaatgatgc tgctccccaa
    17221 ccagagggcc ctcctccctg attttttcca tctttggtat agagaaaata aagagtccca
    17281 tcttcattta ttttcttagc aattctttta agaatatcta cagcatattc ttctcttcca
    17341 tgttgaaacg cagcatgtgc taattcacca gcaacaaaag aagcaatacc accattgata
    17401 taactgccgg ctgaataagt ataaaattga ggatatggtg gatataaact aaaccattct
    17461 gcaaaatact caccttttag taaatcccta agtttttggt attcatctaa aatagaaaca
    17521 gccattttat gagttggaag accacgattt atatcataag cattagaaag gcttaattgt
    17581 tctctttcat ctactccata gaaggcatta tctaatggta aatggtgaat ataaaattta
    17641 ccgttccata gggtaatgtt ggctcttcct cttaattctt ctgctctttc tttccaaaaa
    17701 gatgattttc tttcttgtcc acaaatttca aacatttttg ataattgtat cattgcagca
    17761 taaagtcctg aattatcacc atgcataata cccattggat cgtcaggaag aatacggcgg
    17821 ttaatcccag tatcaggtct attaataaaa tcccatgtat caatagtcct tggacgtttc
    17881 ataagtttgt atttattatc ccatcgttta gggtctgaaa gacagtattt tatacctttc
    17941 tcaagatgat ttagattttt ctctaaccat tttgtatctc cagttgcttg ccaaatacga
    18001 tgaactccct ctactattaa atactctata tctgcttcaa cttccaatct aactaatcca
    18061 tatcctaaat ctggaaaaag gactctacaa tcgccaccta caaagtttaa atggccggca
    18121 attgggctat taaatggagc aaaaatttca taaaaaaaac ctttgttgtt ttggttctta
    18181 agaattaaat ctaaaaaact tcggatttct ttttgccagt aaatatatcc tttcatctca
    18241 tgaatatggt ctcttaccca atgagggttt gttcgaataa attcattttt agagtgataa
    18301 acgcgactat catttgcaac tatggaacga agtttttcat ataaaccgaa aaaagaagat
    18361 tcaacatcta aacacaaaaa tgtctctttc attttttccc cacttgtatt ttggggtact
    18421 gccaaaattg gctatagctt ttataatgca tttctttgtc ttttaagatt tttataagta
    18481 tttatatcca tcctcttttt ttgtttctct tccttcttta acccatcccc ttttgaaatc
    18541 tccccctctg ggcgtagccc aacgggcgag ccttacccct ctttataaaa tccctctcta
    18601 tcccgttgga ctatctgaaa tccccctacc ccctttgtaa aagggggaaa tgacgcacca
    18661 actctgccct gctacccttt gcttccttag tcggtttaat aaagagagaa gaaactcctt
    18721 cttttaacaa ggcatgtccc tcaatgtctt agtgcgatag tcccccaacg tatcagtggc
    18781 ggagagaaga agaaagattt tcttatccac attataattt tacaacaatt ttttttattg
    18841 aaaaaccatt atcttttatt ttttttaaaa tttccttttt tttcatatta agaagagata
    18901 gataacaact tgaatcaact ttaatgtaaa gtatgttatt tttcatacca tttacataag
    18961 tatgtccctg taaattatcg tctattattc tgttccatag aacatcaata tcaatttctc
    19021 tgtttttatc ttctgttttc catatatcta caatcctttt tgttatatcc ccaattttct
    19081 ctatcattct attttcaaag gcattgtaac atacaaaaag ttattattaa gttgtggtct
    19141 cattaaaaca ggtgcttttt tatcagtaaa agcaaagacc attttttctt cattgacttt
    19201 ttgtaaaaaa tcaattaaaa attcaggaaa gaaagcaaag ttcatttctt ctccatcata
    19261 cccaatttca aatttttcct gtccctgacc aatttcagga gaaacaacag agaccattat
    19321 tattccttta tttaaagtaa tttttaattt attatatctt tcacttgtaa aaagggatat
    19381 tcttttaaga catgaaagaa atttatcttt gtcaataata gcaatttgac attttttttc
    19441 atcaggaata actacatcat attcaggaaa atcctccact cctgctaata attgagatat
    19501 aagaagaata ttggaaaatt taaaagtaat ttgattttta ccaactgaaa tttctgttag
    19561 ttcatcagtt aataggtctg ttaatatatt taagagttta aaagagagaa gaacttttat
    19621 tttgggagaa ggggtgcttt ctaattctgc cttacttaaa gaaagtcgtc ttccatcagt
    19681 gcctattaag tttatgttat tctcttttat ttctaaaagt cctcctctaa aatatggctt
    19741 ttgttcattt ggattaacac aaaatttaat tttatcaatt gcatctttta atatatttcc
    19801 aggtatatta aaacttacat tagaagatat ttttgaaatg cggggaaaat catcggcatt
    19861 cattgttata aatctgtatt ttatattttc attacttatc tccacttcat tttctttttc
    19921 ttctatttta atttcttcat caggtatttg tttaataaga gaaataaatt gttttccggg
    19981 aattaaaatt gacccctttt tgaattgaca ttttaaagaa acctttatag tattttcaag
    20041 atctgttgaa ataaaactta attcatcttt tccttcaatt aaaattccag ataaaatttc
    20101 cattgatgat tttgaaggaa gaattttaga aacacaattt aaaacactat ccaattccct
    20161 actatttatt attatttcca tttttacctc cattaataat cataattatt gtttttttgt
    20221 tgtattttgt atttaagtta tttattctca agtacttata ttgtggaaaa cctgtggaaa
    20281 atttatacac ttaaccccct tttaatattt tcaacactgt tttttatata tgtatcattt
    20341 tcaataagtt tcttaacttt tttgcaagcg ttgataacag ttgtatggtc ttttatacca
    20401 aaaaattctc ctattgaatt taatgaagag gtagttaagt cccttgctaa atacattgca
    20461 atttgccttg gtattagtat atttttaatt ctttttccac ttttaatttc tttttcagat
    20521 atattaaaat aattacaaac catttccatt attgtttcaa gagtaatttt tctttttcct
    20581 tttcttttaa atccgtcaat aatctcattt actaattctt catttatttc actattggta
    20641 agttttgaaa gagcaagtat tctatttaat atcccttcaa gaactctgat gttatcttca
    20701 actttttctg caattagaaa tattatatca tcatttatta caatatttct aatttcggct
    20761 ttcttcttta atattgcaac tcttgtttca aaatcagggg gttgaaggtc aacaacaagt
    20821 ccccattcaa accttgatat tattctttct tcaatagcag taatttcttt tggaggtcta
    20881 tcacttgata aaatcatttg ttttttatta tcatagagaa agttaaatgt atgaaaaaac
    20941 tcttcctggg aaccaccttt cccagcaata aaatggatat catcaatcag caggaaatct
    21001 aaatttctaa atttatttct gaaattgtaa gttgttttat ttttaatact ttggatatat
    21061 tcatttacaa agaattcagc agggatataa gccattttta aatcacttct gttcttgata
    21121 taatgaccta ttgcttgcat tagatgtgtt tttcctaatc caacttttcc atatataaaa
    21181 agaggattgt atgcaatgcc aggtgattga gcaactgcta aagcagcagc atgagcaaga
    21241 cgattacagg gaccaacaat aaaattttca aatgtatatt ctgggttcaa attaatttca
    21301 agaagtttcc tatcactttt ttcctcttct ccataagata tatctatttt tgggtaaaaa
    21361 ccatataatt ttgaaaattc ctcttttatt atttcaagaa atgggaaaaa tccccgataa
    21421 aatgctctat tgggaatttg aatatgtaaa atttcaccat caaaatcaat gatttttact
    21481 ggtttaatcc atatatcata acctcttgga ttgttaattt gttcttttat attattaaaa
    21541 atatgttgag agaggttttc cacctcaaat ttttccattt tccacaattt ttccacatct
    21601 tttatttatt catttttccc acaacaatgt ttatattttt taccacttcc acatggacat
    21661 gggtcattcc tacctatttt tttacctgaa acagcgggag tttgttttac cattacaggt
    21721 ggctgtgcat ttggtccttg gattatcgct tgttgttctc cttcttgtgt tatagtatca
    21781 aattgctcat attgtttatg tataaagtca gcacttacct tttgtttttc agcatgcatt
    21841 gtaaattttt catcacttac ttttatatta aatattgcag atattccttc ctgtttaatt
    21901 gcagaaagca tctgttgaaa taattgatat ccttcatgtt tataagaaag aagagggtcc
    21961 ctttgagcat atacccttaa atttattcct tctcttaact catcaatacc tcttaaatgt
    22021 gctttccatc tattatctat tacctgtaaa aagataaggc gttgtatttc ataaaagaaa
    22081 ggacctattt ctttttgttt ttcttctaaa acagaaaata ttttgttttt gatatcctgt
    22141 aagagatttt ccttgaaaac aacagtattt gatatgttct caacagaggg aatttcagca
    22201 tctatattaa aaattccttt tattgcaact tttaacccat caatattcca ctggaatgat
    22261 ttaacactta aactacaaaa ttcatcaaac agattattta taatttcctc aataaactcc
    22321 cttatataat tatcaatatt tctcccttca agaaagtcct gtctgattga ataaataaca
    22381 tttctctgtt catttagaac attgtcaaat tcaactaatt gttttcttat ttcaaaattt
    22441 ctaccctcaa cttttttttg ggcattattt atcatttttg ttattagagg atgagtaatg
    22501 ttctcgccct ctggcatttt tcccataatt cccataagac gttctgaacc aaaaattctt
    22561 aaaaggtcat cttcaagaga aagatagaac tttgaagacc ccgggtcccc ctgtcttcct
    22621 gctcttcctt ttaactgatt atcaattctt cttgcttcat atctttcact ccctacaaca
    22681 tgtaagccac caagtgatac aactttttgg tgttcttctt cccatggttt tttatatttt
    22741 tcaagtaatg tctcatatat ttctttaaat tcttcaggac ttttttctaa ttcttctctt
    22801 gctttttgcc attggtcttc aattttcctt ctttgttttt catattcatc tctttctttt
    22861 tttattattt cttcataatt tttatgtagt tgctcatact cgtttaaaat tgaaagatat
    22921 tctttttcaa gagatggcag gtttggaaag tgttctgtaa aaattttttt ctctgcctgt
    22981 tcccaatcct ttaagtaatt ttcatataat ttttcttctt ctttttcaaa ttgctgataa
    23041 atttgctcaa tattttcttt cttttctgtt gtgtttttaa taaatctggt gagaaggtca
    23101 atatattttt cagttgtttt cttttgattt tcaagattta aagaaggata tgttttttca
    23161 acaagagatg aatatttttt taatgaatca atataattat taatttgtgt tgtcaaattc
    23221 tttataaaat caggtgtatt tttaaaattc cctaaaattt ctaccaactg gtttctttct
    23281 tcatcaatat cttcttttaa aacaatatca tattttttct ttatggattt tttataaaga
    23341 atatagtccc tatatgcttt tccagctgtt tctcttggtt cttttaatcg ttctggtttt
    23401 tctttgaact tttcttgaag agaaagtaat aaagaattct cgtttttata cctttctttt
    23461 aatttttcaa gtcgtgtgtt gattttatca aaagatgctt caattgtttt cttaaaaatt
    23521 tctttttcaa aaatttcttt tgctttacct tctaagtcag aatatatttt ttctttttca
    23581 tttaatgaac tttttaaaaa agaaagtttt tctccatatt ccttttcagt cacatcaatt
    23641 ttatttttat atcctttttc taagtcctct aagacatctg aataatcact ttttttaaca
    23701 atgtctttac tacctttttt actccatatt gtttttattg tttcttctct tgcaattatt
    23761 tcaggatttc ctccaagaat aatatcaact cctcttcctg ccatttgtgt tgctattgtt
    23821 actgctttaa atttacctgc ctgtgcaatt attcctgctt cactttcgtg gttttttccg
    23881 tgaagaacat tatgtggaat attatttctt ctcaaatatc cacttaattt ttctgctttt
    23941 tctattgaaa tcgtcccaac aagaacgggt ctgcctattt catgcatttg ttttatctca
    24001 ttggtaactg atataaattt ttctttttca gttctataaa tttcatcatt atattctgtt
    24061 ctgctaagtg gtttatttgt tggaacaaca attacatcta atttataaat ttctttaaac
    24121 tctattgctt ctgtaacagc agttccagtc atacctgcta ttttttcata tagtttaaaa
    24181 taattctgaa aagaaatagt tgcaagtgtt tgattctcgc tttcaatcgg gactccttct
    24241 tttgcttcaa ttgcctggtg aagtccatcg ctccattttc tccctggcat caatctacct
    24301 gtgaattcat caactattat tacctgccca tcttttacaa catattcttt atctctttta
    24361 aaaaaagtat gaccttttaa tgcctgattt attttatgta ctatttcagt atgacctcca
    24421 tcataaagat ttttaattga taataacctt tcacattttc ttataccttc ttctgttaaa
    24481 acagcagttt gtgcttcttc atcaacaata taatctgtat ctcttacaag ttttcttacc
    24541 aatcggtcaa tttcataata aagttgtggt ttttcttcag aaggtcctga aattattaga
    24601 ggagttcttg cttcatcaat aaggatagag tcaacttcat caattattgc ataataaagt
    24661 tttctctgaa cctgttcttc ttttcttata gccatgttgt ctctgagata atcaaatcca
    24721 aactcacttc ctattccata ggttatatca cattggtatg ctttttttct ttcaacagga
    24781 taaagataaa gaaaccttga atcagcagga agatatgtta ggtcaaattt aaaagcagaa
    24841 gaagaggaaa gtcccttacc ttctttataa gaaataatac aaccaaccga aagtcctaaa
    24901 taatcaaaaa ttggtcccat ccattgagta tctctttttg ctaaataatc atttacagtt
    24961 acaagatgac agccattgcc ttggagagca tttaagtaaa gaggtaaggt tgcaacaagt
    25021 gttttacctt caccagttga catttctgca attttacctt tatatagaac tattcctcca
    25081 ataatctgaa catcaaaatg tctcatattt aaagttcttt ttgcagcctc tcttacaaga
    25141 gcaaatccat caataatgaa attatcaacc ggctctccat cagatattct tttcctgaat
    25201 tcttctgttt ttcccctcat ttgctgtttt gtcatattgg caacttctcc ctctttctca
    25261 tttatttgct ttaagagggt aatacattca ttaagaaaag aatatttctc tttattaaat
    25321 attctattga taaaatactt tgttccagga tttaaaattt taagtttttc taaaaggtct
    25381 tcataatatt ccattcctat ctcctgtaaa aatgcaataa acaaaattag caacgaatcc
    25441 cctctgcctc cctttgtaaa ccgactaaag aagcaaaggg tagcaaagca gagttggtgc
    25501 gttatcgttt cccagatagc acaactgcta ccgactaaag ttgcttacct tgtgcctcag
    25561 agttggtgcg ttatcgtctt ccagatagca caacggggta gggggattgt tctacacagg
    25621 cagtttaagg aaagcatacg aaccagccct accc
//
LOCUS       DAOOVW010000024        25634 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_1292_length_25634_cov_6.940303, whole genome shotgun sequence.
ACCESSION   DAOOVW010000024 DAOOVW010000000
VERSION     DAOOVW010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 25634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25634
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1292_length_25634_cov_6.940303"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="PLW95_04585"
     CDS             complement(<1..462)
                     /locus_tag="PLW95_04585"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOV21940.1"
                     /translation="MNLLWIFFTSFFVAFSGALSPGPLLAVTIVETYKNGVKTGPLLI
                     SGHAFLEFLLISIFFTGITQFLKNQLVFKIVAFFGCLVLIYMGFSMLIKKGLYMKEKG
                     GKKNFSLPLKGIIASLSNPYWFLWWLTIGLTYFTISLKFGVKGVFLFFVGHI"
     gene            complement(459..1217)
                     /locus_tag="PLW95_04590"
     CDS             complement(459..1217)
                     /locus_tag="PLW95_04590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOV21941.1"
                     /translation="MKRIYLNRNDWLIEKDNVKIRGETYHYLKNVLRMKPNDIFAGFD
                     GSGKEYLIEVSKMVKGIIYGKIIKAEKKFDIETPFDICLFQAVPKGKKMEQIVEGTAQ
                     LGVKKIYPVISKRVIVNIEDKKKEEKKIERWKKVCQQASKVSGRVIVPDVVSFMNFND
                     AIKIQKDIGIVFWEGESRILRDIIVEEKIKITENKKIHIFIGPEGGFDEEEISLAKEN
                     NFLISSLGKRILKVEIASIVATAIVIYEFEQILK"
     gene            1256..1567
                     /locus_tag="PLW95_04595"
     CDS             1256..1567
                     /locus_tag="PLW95_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21942.1"
                     /translation="MSNLKILIKWCFIYFAGLIVNKFISQFILYIFCIVGFLTGFILR
                     VVGKYPGKTEPLILDFSAFLLAIVILFLNKSINFKIGLFLIFLPHIIYNLVLISFILI
                     F"
     gene            complement(1551..2153)
                     /locus_tag="PLW95_04600"
     CDS             complement(1551..2153)
                     /locus_tag="PLW95_04600"
                     /inference="COORDINATES: protein motif:HMM:NF019749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane lipoprotein lipid attachment
                     site-containing protein"
                     /protein_id="HOV21943.1"
                     /translation="MKKIFFLLLIFVILTGCQKKKENYNLPLSDKEIEECLNFGKDNF
                     SLSYTEFTENWAVNLGYDYGKGRAILITPFLKVALIGKKAAENNEKVDIDMVEKILAK
                     ENDKISFEVTLFGNYPQFGRTVNAKLKYKDKEFSPTYCFFPKFSQVARDYTQIVTGKI
                     VFPKDDIPDDAQISLIVSFKPFENEKEETCTFDFDLKRLK"
     gene            2244..3773
                     /locus_tag="PLW95_04605"
     CDS             2244..3773
                     /locus_tag="PLW95_04605"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012675437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOV21944.1"
                     /translation="MEKIVIFDTTLRDGEQSPGASLTSEEKLKISFQLEKLGVDIIEG
                     GFPIASEDDAKAVKEIGEKIKKSSVCALARCKNQDIDVALKSLEKANKPRLHLFLATS
                     EIHRKYKLNKAKSEIVRIAKESVRYAKKFIDDIEFSPEDASRTEIDFLIEVSKAVIEE
                     GAKTINIPDTVGYSNPWEFGEMIKMLKENLPDDIILSVHCHNDLGLAVANSLSAILNG
                     AKQIECTINGIGERAGNASLEEIVMNLKVREDYFKFTTDINTKEIYKTSRLVSSLTGI
                     PVQPNKAIVGENAFRHEAGIHQDGVLKYRGTYEIMSPELIGLQGDQLVLGKHSGRHAL
                     KSRLQDLGFDIKNSELEKVFQRFKQLSDKKKEITDIDLIVIAEEELTPLKPFYSLVYF
                     HIISGTSTIPSATVRLEKEGKIYEDASSGDGPVEAIYRAIDRITGLTPTLKEYKINAI
                     TGGKDAQGEVVVTLEIDGIRTSGKGVSTDVIEASAKAYITAINYYISKKDLIKKAYKG
                     T"
     gene            3775..5031
                     /gene="leuC"
                     /locus_tag="PLW95_04610"
     CDS             3775..5031
                     /gene="leuC"
                     /locus_tag="PLW95_04610"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOV21945.1"
                     /translation="MTITQKIIAKHCDKSDVLPGEFVEGNVDIILANDVTGPLAINEF
                     EKIGAKDVFSSEKIVLVPDHFTPCKDIKSAELVKTLRNFAKKYKVRFYEIGKVGVEHA
                     LLPEEGLTLPGNLIIGADSHTCTYGAVGAFSTGVGSTDVAAAMVMGKVWLKVPSTIKF
                     YYYGNLSKYVGGKDLILYTIGRIGVNGALYKSMEFTGPVIENLPMDDRFTICNMVIEC
                     GGKNGVIPPDEKTIQFTKEVSGKEIDISWIKTDTDNDYEKIIEINCNEIQPQVSAPHL
                     PSNSKDVREFSNVKIDQVFIGSCTNGRISDLRKSAKIFKGKRVHPEVRCIVIPATQKV
                     YKQALKEGLIDIFLDSGCVIGPPTCGPCLGGHMGVLGKGEVAIATTNRNFVGRMGHPE
                     SYVYLSNPEVASASAIKGKITHPDEI"
     gene            5031..6047
                     /locus_tag="PLW95_04615"
     CDS             5031..6047
                     /locus_tag="PLW95_04615"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOV21946.1"
                     /translation="MKNWEKKETFLKIIQRKGKGIIPARVSIIATIWDSDPEFFENLK
                     KEYPDVSLNVSRNLEREKENFKKDQWGCIWHFPGNYLDGQVIEHPLSSWDLFKNFTPP
                     NPAEHRDWILTKKEIEEVKKEGGFSSVGVEHGFFYLRLTYLRGFNNLMIDIGSKEPKL
                     KELIKILTEYWKDVVKRLVELKPDMISFGDDLGHQNTLPINPQTWRELIKPSYKEIFS
                     ICREKGIEVYLHSDGYIVDIIPDLIDVGVTILNPQDLVNGLDYLEKIAKGKVTIDLDI
                     DRQKITVFGKPKNIENHIKNCVEKLGSKNGGLLLLYGAYPGTPKENIAAVIKSMQKYH
                     NFWV"
     gene            6075..6917
                     /locus_tag="PLW95_04620"
     CDS             6075..6917
                     /locus_tag="PLW95_04620"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOV21947.1"
                     /translation="MKIYDEKTYLVQQNTSNLKTIEIKEKMIKHLLSKYFIKNMNDWA
                     GIESDLIIADPPFGIKFSGKPTNYHRNAENVVDGYVEWGVSEYGKKIQELLECIKRNL
                     KENGQALIFSGWNNSNIIQDILMRFKGLKLRSKMYWMYNFAPVCRKKPSHNVYEIFWI
                     TKSEKWTYNKRCNTEHCQIGEPNLTTLNFKRDYKVNMPKYPTRLPFKLLQCLIEHFSN
                     KNDLIFDPLCGSGMVGVVSYILKRDFIIGDLNKNGKFVFKYLLDYYLNKNGLIQDTQE
                     NYIA"
     gene            7254..7751
                     /gene="leuD"
                     /locus_tag="PLW95_04625"
     CDS             7254..7751
                     /gene="leuD"
                     /locus_tag="PLW95_04625"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011012075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOV21948.1"
                     /translation="MKIKGKVWKFGDHVNTDDIISARYLNTTDEKELASHCMETIRPD
                     FVKKVQKGDIIVAGENFGCGSSREHAPIAIKECSISVVIAKSFARIFFRNSINIGLPL
                     IELEEVNRINEGNILEIDLENGIINNLTNGEIYKFSKYPEFLQEIVKNGGLMQWVKKK
                     YIKSV"
     gene            7718..8800
                     /locus_tag="PLW95_04630"
     CDS             7718..8800
                     /locus_tag="PLW95_04630"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOV21949.1"
                     /translation="MGKKEIYKIGVIGGDGIGPEIIRQGIKVLQAVGERENIKFEFIE
                     YDFGGERYLKTGETLPSSAIDEFRKLDAIYLGAVGHPDVQPGILEKEILLKIRFELDQ
                     YINLRPVKLYPGVWCPLKDKNEKDIDYVIVRENTEGLYCGIGGNFKKETKDEVATQLE
                     INTRKGVERCIKYAFEYARKRNKEKKLTVVDKANVLTYAGDLWRRTASQLSCNYPDIK
                     LDFAFVDATCMWMVKNPEWFDVVVTNNMFGDIITDLGAITQGGLGIAAGGNINPEGVS
                     MFEPIHGSAPKYAGKNVACPLATISAGGMMLETLGEEKAANLIEKAIIKALGEGYIKS
                     LEAGKMGLTTDEVGDLISKFVKEINE"
     gene            9074..9574
                     /locus_tag="PLW95_04635"
     CDS             9074..9574
                     /locus_tag="PLW95_04635"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009368291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOV21950.1"
                     /translation="MVDYRKLFKSCGENVIIEDSVIIYQPEKMSVGNNIKFYHGVYIE
                     VGNLEKSFIEIGDNSHFAPYTILYGGRGLKIGWGVCVAAHVVFATIGEGYEKADIPMY
                     CQKPSSGGPIIVEDNVWICANAVITANVTIGTGSIVGAGAVVTRDVEPYSIVGGVPAR
                     LIRKRK"
     gene            9817..11166
                     /locus_tag="PLW95_04640"
     CDS             9817..11166
                     /locus_tag="PLW95_04640"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOV21951.1"
                     /translation="MEKDVVVIGGGPAGISASWAAAKSGSSVLLIERYGFLGGMATSG
                     LIGPILGHYLSEKKPSVAGFLKFLVEKISEFDGCEKWESACKKGGIPFDTEIFKYASE
                     LLLLEQNVEILYHSFIISSKTENGEIKEIEVANKSGIQKIRGNVFIDTTGDADVTYLS
                     GFECTKGRPQDGKMHSMGTIFKVGGIDEKKLTEEVIKDASEKLKILREKGELKIWNTG
                     LGGKGSTIRKGERSFNITRFSGDGTNINDLTKGEIELREQTFKLFEFLKKNVSGFENA
                     YISALPANIGIRETRQIKGLYTLIDEDIISGKKAQDSIARGTYMIDIHCPLGRTKNNV
                     HLCVKECSTKEYCIMLDKYMDYLPSKEKLYPPINDYYSIPSRCLIPLGAKNLVVGGRC
                     ISAEHKAMASIRVMAICMATGQAAGIIASLSSKNNTPVNKVNIKEVQKIITQQEGIY"
     gene            11169..11393
                     /locus_tag="PLW95_04645"
     CDS             11169..11393
                     /locus_tag="PLW95_04645"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOV21952.1"
                     /translation="MIKLSDTKEGIYKIVNIEKCGKGRLKKLYNLGIFVGDVIEVIKP
                     GPGPVIIKKGNTRIGIGNGIANDIIIEPID"
     gene            11449..13437
                     /gene="feoB"
                     /locus_tag="PLW95_04650"
     CDS             11449..13437
                     /gene="feoB"
                     /locus_tag="PLW95_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOV21953.1"
                     /translation="MEEKTIAISGNPNTGKTTIFNTLTGLRQEIGNWPGVTVEKKEGY
                     FIYKNIKFNVVDLPGTYTLIEESEDQKIAIDFLTNEKVDTVILTANASNLDRSLYLLI
                     LLSELNQNIIVVLNMIDIAEKEGIKYDIEKLEKIFGIPFVETIANKGYGIENLKEKIY
                     EKTRDKREKYFFKINYGEEIENYVSKIEGKLQNISLPYPSKFISLRLLEKDERIRELI
                     KNKVNINEIDEIIGQLEKKIPNIEVILIEKRYGIINGMVKECLLSRKKVEKRIEITNY
                     LDRVLTNNFLGPLIFLLIMFIIFQIVFKWGAPFQELLDKFFHFSGEKISLFLHSINAP
                     LFLISFIEEGIISGIGSVVMFLPNIFLFFLLFSILEETGYMARAAFTTDRLMHKMGLH
                     GKSAIPLILGFGCNVPAIMSTRTLETKKDKILTILVIPFMSCSARLPVYILFTGIFFK
                     SHQSLVIFSIYLIGILLGILMARILKFIFFKEESIPIIIELPPYEIPYWKNVIIEGWE
                     RTKVFLKRAGTIIFVGVLILWFLSYFPDPSNFGKETSLIGRIGNFFAPILKPAGFGFW
                     QASVALIFGIYAKELVVGCFGTLFGENNLSTILPYYFTPASAYSFMLMTLLYIPCLAT
                     IAIIKREIGIKWALFSAIFSIFLGYLISTLFYQLTLLF"
     gene            complement(13542..14066)
                     /locus_tag="PLW95_04655"
     CDS             complement(13542..14066)
                     /locus_tag="PLW95_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21954.1"
                     /translation="MKDRLYFLVEGPDDERFFDKIVKPKFKNRYYEIIILKYAEKTKE
                     GVKNIILSIKKIPMTDYIFVVDINDSPCVTAKKQQIWEKFSYVDLDRIIVVIKKIESW
                     YLAGLDNANAKKLKISSLETTDNVDKGKFKSLVGKESDLKDLKIEILKHFSTEVAMEK
                     NKSFKYFIEKYNCK"
     gene            complement(14063..15301)
                     /locus_tag="PLW95_04660"
     CDS             complement(14063..15301)
                     /locus_tag="PLW95_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014041795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOV21955.1"
                     /translation="MAIKKIKLSNFKSFRDMEINLGKFNVLIGANASGKSNFIQIFRF
                     LKDIKNFGLDNAISMQGGIEYLRNMNIGSSDNFSLEVISDQVYMRPFMAKKPEKKFIR
                     AGVYETIYNFTIKFRKRGLGVETEEQIVQKFNIEVQQERIGKEKKKEIGEITISKING
                     KINVVPSKLPNEISTDDIIPPFLTIEQNKQKSLLLERVTSFIMPPIFSDISIYDFDPK
                     IIKRATPITGKAELEEDGSNLAIILKNIIENKENKRKFFNLVQDLLPFVDNLNVEKFV
                     DKSLFFKLKETYSKKEYLPASLISDGTVNIIALIIALYFEKKPITIIEEPERNIHPYL
                     ISKVVEMMKDVTTQVIVTTHNPEIVKHAGLENILLISRDKNGFSVISKPQEKEDVKIF
                     LENELGIEELYVQNLLGSNI"
     gene            complement(15380..15757)
                     /locus_tag="PLW95_04665"
     CDS             complement(15380..15757)
                     /locus_tag="PLW95_04665"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOV21956.1"
                     /translation="MKAKEMEKTMEEKIRNIIVEVLTKSNITIDEIILFGSRARGDFN
                     EESDFDILIIIRNNVKINQKRKIWQDIFNALHKHFPFTPFDIIIKTLNDFESEKNVVN
                     TISNEAYIEGEIIFYKAERYYGI"
     gene            complement(16112..17188)
                     /locus_tag="PLW95_04670"
     CDS             complement(16112..17188)
                     /locus_tag="PLW95_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015591010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HOV21957.1"
                     /translation="MKEEYKKLEEVGYDTEEKERAGSFVQEDNNVLLAGIKEENLEIS
                     SIENALEKYPEVKEKYFGKAFEMFNIHYDKNTKGGYFIRVKKGKKVLFPVQACLILKT
                     EKFKQKVHNIIIVEEGAELYLINGCTSLKAANEAYHLGISEFFIHKGGFLNFTMLHSW
                     KKDVEVEPKSVAVVEEGGVFISNYMNFKPVKKIVMYPTAILKGKNSRVRFNSIVVGFP
                     DCYQDIGNRVIFAERATSAEVISRAISLGGTVISRGNLKAISPQVKGHLECKGLVLSE
                     RGKIIAIPELETDFQDVELSHEAAIGKIRKEEIEYLASRGIPEEEAQSIIIRGFMNTE
                     ILGLPEVIKNEIDKITSELTQSPL"
     assembly_gap    17420..17519
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(17646..18365)
                     /locus_tag="PLW95_04675"
     CDS             complement(17646..18365)
                     /locus_tag="PLW95_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOV21958.1"
                     /translation="MLKVDKIIVTSEEGDEILKELSLTIKDGDIVIMFGPNGSGKSTL
                     IKSIMGVSDYKIKEGDILFDGKSLKNLTVSERAKMGIGVMYQQPPEISGVKLYQIAKY
                     LEENDDVIFDYAEKLNLKEFLDRDVNVDFSGGEKKRSELFQILLQKPKLLLLDEPESG
                     VDIENISIMGKVLNEFFKKNGSSALIITHTGYILQYISAKRGCVLMNGKLCCSDEPEK
                     IFQDIRKNGYEKCGKCKCEDQ"
     gene            complement(18358..19200)
                     /locus_tag="PLW95_04680"
     CDS             complement(18358..19200)
                     /locus_tag="PLW95_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOV21959.1"
                     /translation="MDNELKILKKFFETKPQIVMAFLFGSRATGQQRNISDWDIGIYF
                     KPVEYMELEANFEYPEEEKIWGELVEILQTDNIDLVVLNRASPDLVYKILRDGLPLKI
                     SDKKLYLNLLCKVSYEAMDWWKFVDEYYWISEKAKSISPENKAEILKYLRFLENEFTD
                     IEIMKNITWQEYNQNSIKRRALERWIENLVMGCLDISKIILASEKKTIPQSYKETLKV
                     FCTVLGFSPEEAEEFSALAQYRNIVVHEYLDIRWRRMQKFILLVEKLFPTFLERVKKV
                     IKNA"
     gene            complement(19287..20981)
                     /locus_tag="PLW95_04685"
     CDS             complement(19287..20981)
                     /locus_tag="PLW95_04685"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOV21960.1"
                     /translation="MLSDLEIAQKAEIKHIGEIAEKIGIDNKFLELYGNYKAKVSLDI
                     LEKIKDRPDGKYIDITAITPTPLGEGKTVTTIGLSQAIGKLNKKVFTCIRQPSLGPVF
                     GIKGGAAGGGYSQVIPMDDFNLHLTGDVHAVGIAHNLLAAFVDNSVFKGNPFNIDPLT
                     INWQRVVDVSDRFLRHIVIGLGGEENGIPRETNFDITVASEVMAIIALTRDIFDLRKK
                     LGKIIVAQTKDGKPVTAEDVKCAGAMAVLLKDAIKPNLMQTIEHTPVFVHAGPFANIA
                     HGNSSIIADLIALKLSDYVVTESGFGADCGMEKFMDIKCRYSGLRPDCVVIVCTVRAL
                     KMHSGKFKVVPGKPLDKGLIEENIKALKEGSCNLQKHIENAKYFGIPVVVAINKFKTD
                     TEKEIETLKELSLKYGADDVEVSEVWEKGGEGGIGLAEKVIKASEKEKNFKFLYEDNW
                     DIKKKIETIATKIYGAGKIEYSPLAEKKIKTYTKWGFGNLPICMAKTHLSLSHDPNLK
                     GKPDGFTLPIRDIRVSAGAGFIYPLCGEMRTMPGLPSHPAGEKVDIDKDGKIVGLF"
     gene            complement(21356..24490)
                     /locus_tag="PLW95_04690"
     CDS             complement(21356..24490)
                     /locus_tag="PLW95_04690"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOV21961.1"
                     /translation="MEIKKIIEGFKEPGCYYRGAPFWAWNGKLEEKELRWQIRMMKQM
                     GLGGFFMHSRVGLDTPYLSEEWFSYINACADEGEKLNMLAFLYDEDRWPSGAGGGIVT
                     KNKKYRMRYLDMKILDDVNEIKNIKDVKGIFIADFEGPYIKEYRKVKKGEEIKLKKSE
                     KILIFSIKIQKESDWYNGQTYLDTLNPDAVKEFIKVTHQQYKKYCGKYFGSTIPGIFT
                     DEPNYGTSFFLEDNGIDTPWTEKLANVFKKKYGYDILNFLPEIYFNVVGEKISKVRYN
                     YFDCITFLFTTSFSKQIGKWCEENNLLFTGHVLAEDTLISQTSVVGSCQRFYEYMQAP
                     GMDLLTEYRKFSNTAKQVSSVARQFGRKWRLTETYGCTGWDFPFLGHKALGDWQVALG
                     INLRCQHLSWYTMLGEAKRDYPASIFYQSPWWNLYKKVEDYFARINFIMTNGNEVRDL
                     LVLHPIESMWAIYRNKNLIEKNLEKAEEVVEYDKMFEKLCESLLSLHVDFDYGDEEIL
                     SRWAKVKKGKETKIKVNKAEYKAVLVPPLYTIRNSTLKLLKEFKKEGGEVIFIKNFPE
                     YIDGEKANFDFSSIFDIGENIEDGLKKIEKKARRISILDEKGKEIQPILYLLREDDDF
                     YYLFLCNTSDEWKKEKFPPVRERNMKFDNVIIKGFPEFKYQPLEFDQENGNVYKCDIN
                     KTENGYEIKTNFAPLGSKLFLIPKKEISIECKKKEKLQEIERKEIKKDKWEIKLSEDN
                     VLVLDMPYYKIGQERWNEKDEILRVDRKVRRYLGIKERGGAMVQPWVRKINKNPKSVD
                     IHLKYEFFVDKIPSGELCICIEKPELYKIYINGYMLPEEMESGWWVDKSLRKINFDPS
                     VLKLGKNEIILQTKYNENHPGLEIIYIKGEFGVKIEKNNDLRIVNKPEEIEIGDWTEK
                     GIPFYSGNLTYMTKLKIENDNKRIFVRIPEYRGTALRIFVNGQQAGIIAWQPNEVEIT
                     EFLSKNDEEIILGIEVIGSRRNSHGPFHWFEKWSEWTGPGQFTPGEKEYYNGYQIVPS
                     GLLKTPEIVFYQEI"
     gene            complement(24481..25290)
                     /locus_tag="PLW95_04695"
     CDS             complement(24481..25290)
                     /locus_tag="PLW95_04695"
                     /inference="COORDINATES: protein motif:HMM:NF013397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HOV21962.1"
                     /translation="MKLFLSPLEISNELEKIVIKKAESTIFELLFFGILAGIYIGFGA
                     HASLTILSGKNVDPVILKFLAGSVFSVGLMLVLIPGAELFTGNILMCLGLIDRKYNLL
                     KLIRNWVVVYIGNFIGSVFLSYIVLKSGVLGSFLSGLTPIGDVAVDIAILKINLPLFQ
                     SFCRGILCNMLVCLAVIMCISSLTIEGKIFGIYFPIMVFVVSGYEHSIANMYFLPLGL
                     MVNGQFLKNFLLIWKNLIPVTLGNIIGGTLIVIFHPKMIRRIFIKKERKWK"
     gene            complement(25287..>25634)
                     /locus_tag="PLW95_04700"
     CDS             complement(25287..>25634)
                     /locus_tag="PLW95_04700"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOV21963.1"
                     /translation="AGAKFFQTQAVYDINIFEKFIKMTENYNVPVLCGIVLLKSAGMA
                     KYMNEKVAGVSIPEKYIKMIAEAKKEERTKVSIKIAVEIIKEVKNMCNGIHIMPLGWD
                     KYVPEVLEHSGVL"
BASE COUNT         8862 a         3990 c         3658 g         9024 t
ORIGIN      
        1 aatatgccca acaaaaaata aaaaaacacc ttttactcca aatttcaaag aaatagtgaa
       61 atatgttaat ccaatagtta accaccacaa aaaccagtaa gggtttgaaa gagaggcaat
      121 aattcctttc aatggtagag agaaattttt ctttcccccc ttttctttca tatacaggcc
      181 cttttttata agcatactaa atcccatata aataagaacc aaacaaccga aaaaagcaac
      241 aattttaaaa acaagttgat tttttaaaaa ttgagttatt cctgtaaaaa atattgaaat
      301 taacagaaat tcaagaaaag catgtcctga aataagcaat ggtccagttt ttaccccatt
      361 tttatatgtt tcaacaatcg taacagcgag aagaggacca ggggacaaag caccagaaaa
      421 agcaacaaaa aaagatgtaa aaaaaatcca taataagttc atttcagtat ttgttcaaat
      481 tcataaatta caattgcagt tgctacaata gaagcaattt ctacctttaa aattcttttt
      541 cccaatgaag aaattaaaaa attattttct tttgctaatg aaatttcttc ttcatcaaac
      601 ccaccttctg gtccaataaa aatgtgaatt tttttatttt ctgtaatttt tattttttct
      661 tcaactataa tatctcttaa aattctactt tccccttccc agaaaacaat tcctatatct
      721 ttctgtattt tgattgcatc attgaaattc ataaaagaaa ccacatcagg aactatcact
      781 cttcctgata ctttgcttgc ctgttggcaa acctttttcc atctttctat tttcttttct
      841 tctttttttt tatcttcaat attaacaata actcttttac ttataacagg ataaattttt
      901 tttactccta attgggcagt cccttctact atttgttcca tcttttttcc ttttggaact
      961 gcctgaaata aacaaatatc aaaaggtgtt tctatatcaa attttttctc tgcttttatt
     1021 attttcccat aaataattcc tttaaccatt tttgaaactt caattaaata ttcttttccg
     1081 gaaccatcaa atccagcaaa aatatcattt ggtttcattc ttaaaacatt ttttaaataa
     1141 tgatatgtct cccctctaat tttcacatta tctttttcaa ttaaccaatc atttctattt
     1201 aagtaaattc ttttcatctc agtttccttt ttctttaatg tagtataata tttatatgtc
     1261 aaatttaaaa attcttataa aatggtgttt tatatatttt gctggtttaa tagtaaataa
     1321 attcatttct caatttattc tttatatttt ctgtattgtt ggttttttga cagggtttat
     1381 tttaagggtt gttggcaagt atccaggaaa aacagaacca ctaattcttg atttctcagc
     1441 ttttttgctc gcaattgtaa ttttattttt aaataaatca attaatttta aaatcggact
     1501 ttttcttata tttttaccac atattattta taatttagtt ttgatttcat ttattttaat
     1561 cttttaaggt caaaatcaaa tgtgcatgtt tcttcttttt cattttcaaa tggtttaaaa
     1621 gatacaataa gagaaatttg ggcatcatct ggtatatcat ctttaggaaa tacaattttc
     1681 cctgttacta tctgagtata atctcttgcc acttgtgaaa attttgggaa aaaacaataa
     1741 gtgggtgaaa attctttatc tttatatttc aactttgcgt ttacagtcct cccaaattga
     1801 ggataattac caaaaagagt aacttcaaaa gatattttat cattttcttt tgcaagaatt
     1861 ttttcaacca tatcaatatc aactttctca ttattttctg ctgctttttt tcctattaaa
     1921 gcaactttta aaaatggggt aattaaaatc gccctgccct ttccataatc atatccaagg
     1981 ttcactgccc aattttcagt aaattcagta taacttaaag agaaattatc ttttccaaaa
     2041 ttaagacatt cttcaatttc tttatcgctc aatggtaaat tataattttc cttttttttc
     2101 tgacatccag ttaatattac aaaaattaat aatagaaaaa atatcttttt catattattt
     2161 attataaatt ttttaaaaat tttcgtcaat tattattgcc aaaaatacct cttgtgtggt
     2221 atgataaatc attggaggaa aaaatggaaa aaattgtaat ttttgataca actttgagag
     2281 atggtgaaca atctccaggg gcgagtttaa caagtgaaga gaaattaaaa atatcttttc
     2341 aacttgaaaa attaggagtt gatattattg aaggtggttt tccaattgca tcggaagatg
     2401 acgcaaaggc agtaaaagaa ataggagaaa aaattaaaaa aagcagtgta tgtgctcttg
     2461 caagatgtaa aaatcaagat attgatgttg ctctaaaatc acttgaaaaa gcaaataaac
     2521 cacgactcca tttgtttctt gcaacatctg aaatacatag aaaatataaa ttaaataaag
     2581 caaaaagtga aatcgtcaga attgcaaaag aatcagttag atatgcaaaa aaatttattg
     2641 atgatattga attttcaccg gaggatgctt caagaacaga aattgacttt ctaattgagg
     2701 tatcaaaagc agttattgaa gaaggagcaa aaacaataaa tattccagat actgttggtt
     2761 attcaaatcc atgggaattt ggagaaatga taaaaatgct taaagaaaat ttaccagatg
     2821 atataatttt aagtgttcac tgccacaatg accttggact tgcagttgca aattcacttt
     2881 ctgctatttt aaatggagca aaacagatag aatgcacaat aaatggtatt ggagaaagag
     2941 caggaaatgc ttcacttgaa gaaattgtta tgaatctcaa agtaagagaa gattatttca
     3001 aatttacaac tgatataaac acaaaagaaa tatataaaac aagccgactt gtttcttctt
     3061 taacagggat accagttcaa ccaaataaag caatagttgg agaaaatgct ttcagacatg
     3121 aagcaggaat tcatcaggat ggtgttttaa aatatagagg aacttatgaa ataatgagtc
     3181 ctgaactgat tggattgcaa ggagaccaac ttgttcttgg aaaacattca ggaagacatg
     3241 ctttaaaatc aagattacag gatttagggt ttgatataaa aaatagtgaa cttgaaaagg
     3301 tctttcaaag gtttaaacaa ttatcagata aaaagaaaga aataacagat atagacctta
     3361 tagttatagc agaagaagag ttaacaccat taaaaccatt ttattctctt gtttattttc
     3421 atattatttc tggtacatca acaattccaa gtgcaactgt tcgtttagaa aaagaaggga
     3481 aaatttatga agatgcttca agtggagatg gaccagttga agctatttat agagcaatag
     3541 atagaattac aggactcact ccaactttga aagaatataa aataaacgca attactggtg
     3601 gaaaagacgc tcagggagaa gttgttgtta cattagaaat agatggaata agaacttctg
     3661 ggaaaggagt aagtactgat gttattgaag caagtgcaaa agcatatata actgcaataa
     3721 actattatat ttcaaaaaag gaccttatta aaaaagcata caaagggaca taaaatgaca
     3781 ataacacaaa aaattatcgc aaaacactgt gataaaagtg atgtcttacc aggtgaattt
     3841 gttgaaggta atgtggacat aattttagca aatgatgtaa caggacctct ggcaataaat
     3901 gaattcgaaa aaattggagc aaaagatgtg ttttcatcag aaaaaattgt tcttgttcct
     3961 gaccatttta ccccctgtaa agatataaaa agtgcagaac ttgtcaaaac acttagaaat
     4021 ttcgccaaaa aatataaagt aagattttat gaaataggga aagttggagt tgaacacgct
     4081 cttttaccag aagaaggact tacactgcct ggaaatttaa ttataggagc agatagtcat
     4141 acatgcactt atggagcagt tggtgctttt tcaacagggg ttggtagtac tgatgtcgca
     4201 gcggcgatgg taatgggaaa ggtctggttg aaagtacctt caactataaa attttattat
     4261 tatggaaatt taagcaagta tgttggtgga aaagacctta ttctttatac cattgggaga
     4321 attggagtta atggtgcttt atataaaagt atggagttta ccggacctgt aattgaaaat
     4381 ttaccgatgg atgacaggtt cacaatttgt aatatggtaa ttgagtgtgg aggaaaaaat
     4441 ggagtaatcc ctccagatga aaaaacaatt caattcacaa aagaagttag tggaaaggaa
     4501 attgatataa gttggataaa aacagatact gataatgact atgaaaaaat aattgaaata
     4561 aattgtaatg aaattcaacc acaggtatct gctccacacc tgccaagtaa ttcaaaagat
     4621 gtaagggaat tttcaaatgt aaaaattgac caggttttta ttggctcatg cacaaatgga
     4681 agaatatctg atttaagaaa gtcagcaaaa atatttaaag gcaagagagt tcatccagaa
     4741 gtcagatgta ttgtaattcc tgcaacacaa aaagtttata aacaggcatt gaaagaagga
     4801 cttattgata ttttcttaga tagtggttgt gttataggac ctccaacctg tggaccatgt
     4861 ttaggaggtc atatgggagt tttaggaaaa ggcgaagttg caatagcaac aacaaataga
     4921 aattttgttg gaagaatggg acatcctgaa agttatgttt atctttcaaa tccagaagtt
     4981 gcatcagcat cggcaataaa aggaaaaata acacatcctg acgaaatata atgaaaaatt
     5041 gggaaaaaaa agaaacattt ttaaaaataa tccaaagaaa aggtaaaggg ataattcctg
     5101 cacgagtatc aataattgca acaatttggg atagtgaccc tgaatttttt gagaatctaa
     5161 aaaaagaata tcccgatgta agtttaaacg tgtcaagaaa tttagaaaga gaaaaagaaa
     5221 attttaaaaa agaccagtgg ggatgtatat ggcattttcc tgggaactat cttgatggtc
     5281 aggtaattga acatcctctt tcttcatggg atttatttaa gaattttact ccccctaacc
     5341 ctgctgaaca tagagattgg atattaacaa aaaaagaaat agaggaagta aaaaaagaag
     5401 ggggattttc atcagtaggt gttgaacatg gtttttttta tttacgactt acttatttaa
     5461 gaggttttaa taatttaatg attgatattg gaagtaaaga accaaaatta aaagaactta
     5521 taaaaatttt aactgaatat tggaaagatg tagtaaaaag attagtcgaa ttaaaaccag
     5581 atatgatatc ttttggagat gacttaggac atcaaaacac acttccaata aaccctcaaa
     5641 catggaggga gttgataaag ccatcttata aagaaatttt ttctatttgt agagaaaagg
     5701 gtattgaagt ttatttgcat agtgatggat atattgttga tattattccc gaccttatag
     5761 atgttggagt aacaattcta aaccctcagg atcttgtaaa tggattagat tatcttgaaa
     5821 aaattgcaaa aggaaaagta acaatagacc ttgacattga tagacaaaaa ataactgtat
     5881 ttggtaaacc aaaaaacatt gaaaaccata taaaaaattg tgttgaaaaa cttggttcta
     5941 aaaatggtgg tttattactt ttgtatggtg cttatccggg aacaccaaaa gaaaatatcg
     6001 ctgctgttat aaaatcaatg caaaaatatc acaatttctg ggtgtaatta aaagttgaaa
     6061 aaaaaggaaa atatatgaaa atttatgatg aaaaaacata cttagttcaa caaaatactt
     6121 ctaatttaaa aacaattgaa ataaaagaaa aaatgataaa acatttatta agtaaatatt
     6181 ttattaaaaa tatgaatgat tgggcaggaa tagaaagtga tttaataatt gctgacccac
     6241 cctttggaat taaatttagt ggaaaaccta caaattatca tagaaatgct gaaaatgtag
     6301 ttgatggcta tgtagaatgg ggtgtatcag aatatggaaa aaaaattcag gaattgttag
     6361 agtgtattaa aagaaattta aaagaaaatg gtcaggcatt gattttttct ggatggaata
     6421 atagtaatat tattcaagat atacttatga gatttaaagg actaaaatta agaagtaaga
     6481 tgtattggat gtataatttt gcccctgttt gtagaaaaaa accatcccat aatgtttatg
     6541 aaattttttg gataacaaaa agtgaaaaat ggacttataa taagagatgc aacaccgaac
     6601 attgccagat aggagaacca aatttaacta ccttaaattt taaaagggac tacaaagtta
     6661 atatgccaaa atatccaaca cgacttccat ttaaactttt acagtgtcta atagagcact
     6721 tttctaataa aaatgactta atttttgacc ctttatgtgg aagtggaatg gtaggagttg
     6781 tttcatacat tttgaaacgg gattttatta taggtgactt aaataaaaat gggaagtttg
     6841 tttttaaata tcttttagat tattatctga acaaaaatgg cttaattcaa gatactcagg
     6901 aaaactatat tgcatgaaaa ttagcaagta ttagaaaaac agagttggtg cgtcatttcc
     6961 ccactttgat aaaccgactg aggtagcaaa gattaacaag cagagttggt gccgtaatgt
     7021 atttcaatag gcacaacggg agttaagggg gatttaggaa atgtaaagtg taaagggaaa
     7081 aatgtaaaat aaaaaacccc ctcactctaa ctcctcgggc gtaagcccta cgggcgagcc
     7141 tctcccctgc gggaagaggg aaaaaagagg ggaatgtatt ttcttctcca cttgggagaa
     7201 aaaataaata accaaaattt tggcagaata caatataaag atggaggaaa aagatgaaaa
     7261 taaaaggaaa ggtatggaaa tttggagacc atgtaaatac tgatgatata atttctgcaa
     7321 gatacttaaa tacaactgat gaaaaagaac ttgcatcaca ttgtatggaa acaataagac
     7381 ctgactttgt aaaaaaagtt cagaaaggtg atataattgt tgcaggagaa aattttggat
     7441 gtggaagtag tagagaacat gctccaatag caattaagga atgtagtatt tcagtagtta
     7501 ttgcaaaaag ttttgcaaga atttttttca gaaattcaat aaatattgga cttcccctta
     7561 ttgaactgga agaagtgaac agaattaatg aaggaaacat acttgaaatt gaccttgaaa
     7621 acggaataat aaataattta acaaatggtg aaatttataa attttctaaa tatcctgaat
     7681 ttttacagga aattgttaaa aatggagggt taatgcaatg ggtaaaaaag aaatatataa
     7741 aatcggtgta attggtggtg atggaattgg tcctgaaatt ataagacagg gtattaaggt
     7801 tttacaggca gttggagaaa gagaaaatat taaatttgag ttcattgaat atgattttgg
     7861 aggagaaaga tatttaaaaa caggagaaac attaccttcc agtgcaattg atgaattcag
     7921 aaaattagac gcaatatatt taggagcagt tgggcatcct gatgtgcaac ctggaatatt
     7981 agaaaaagaa atactcttga aaataaggtt tgaacttgac cagtacataa atttaagacc
     8041 tgtaaaactt tatccaggtg tatggtgtcc attaaaagat aaaaatgaga aagatattga
     8101 ctatgtaatt gttagagaaa atactgaggg actatattgt ggaataggag gaaattttaa
     8161 aaaagaaaca aaagatgaag ttgcgacaca acttgaaata aatacaagaa agggagttga
     8221 aagatgtata aaatatgcat ttgaatatgc aagaaaaaga aataaagaga aaaaattaac
     8281 tgttgttgat aaagcaaatg tcctaactta tgcaggagac ctctggagaa gaacagcaag
     8341 tcaattaagt tgtaattatc cagatataaa acttgatttt gcttttgttg atgctacctg
     8401 tatgtggatg gtaaaaaatc ctgaatggtt tgatgtagtt gtgacaaata atatgtttgg
     8461 agatataata acagaccttg gggcaataac tcagggtggt cttggaatag cagcaggagg
     8521 aaatataaat ccagaaggtg tgagtatgtt tgagccaata catggttctg ctcctaaata
     8581 tgcagggaaa aatgttgcat gtcctcttgc tacaatatca gcaggtggta tgatgcttga
     8641 aacacttgga gaagaaaagg cagcaaattt aatagaaaag gcaattataa aagcattagg
     8701 agaaggatat ataaaaagtt tagaagcagg gaaaatgggg ttaacaacgg atgaagttgg
     8761 ggatttaata tcaaaatttg ttaaagaaat taatgaataa aaaaatagat tatctttact
     8821 tatgtaaaat cagaattaga gggatggaga attaaagaaa aacttgaaac agaagggata
     8881 cccgttatta tccagcaaga agcaacagga aaaatttctg gattaacaat tggtgaactt
     8941 ggagaaataa aagtccttgt cccaatttct tttttgaaac aggcaaaaga aatcttaaat
     9001 attacaaagt attgttaaaa aaataaaatt tcttatttaa ctttgtctga accacatata
     9061 aaaaggagag taaatggttg actatagaaa attatttaaa agttgtggag aaaatgtaat
     9121 aattgaggac tcagtaataa tttatcaacc tgagaaaatg agtgttggca ataatattaa
     9181 gttttaccat ggggtttata ttgaagtagg taatttagaa aaatcgttta ttgaaattgg
     9241 agataattct cattttgccc cctatactat tttgtatgga ggaagagggc taaaaattgg
     9301 gtggggtgta tgtgtagcag cccatgttgt ttttgcaact attggagaag gatatgaaaa
     9361 agcagatatt cccatgtatt gccaaaaacc atcatctggt ggaccaatta ttgttgaaga
     9421 taatgtctgg atttgtgcta atgcagtaat tactgctaat gttacaattg gaacaggaag
     9481 tattgttggt gcaggtgctg ttgtaacaag agatgttgag ccatattcta ttgtaggagg
     9541 tgttcctgct cgtttaattc gcaagagaaa ataattttaa caataatgtc tattcttaaa
     9601 ttacctatca actggactaa tttaaagcag agagtataga gaaatatatc ccgactttct
     9661 cttgaaaatg gaaggagata aaggaaaaaa agtataaagt tttccctttg gggaattagt
     9721 ggtatttgaa gttttaaaat ttagtaaagt aaataaaaaa attttattgc aaaatcttat
     9781 tgaaagtacc tattggttta aaaaaggaga ataaaaatgg aaaaagatgt agttgtaatt
     9841 ggaggtgggc ctgctggaat ttctgcttcc tgggctgctg caaaaagtgg tagtagcgtt
     9901 cttttaattg aaagatatgg ttttttaggt ggtatggcaa cttctggact tattggacca
     9961 atacttggtc attatttatc tgaaaaaaaa ccttctgttg ctggtttttt aaaatttctt
    10021 gttgaaaaaa tatctgaatt tgatggatgt gaaaaatggg aaagtgcatg taaaaaaggt
    10081 ggaatacctt ttgatacaga gatttttaaa tatgcatctg aattattact tttagaacaa
    10141 aatgtagaaa ttctttatca ttcatttatt atatcctcaa aaacagaaaa tggagaaata
    10201 aaagagatag aagttgcaaa taaaagtggc atacaaaaaa taagaggtaa tgtttttatt
    10261 gacacaacag gagatgctga tgttacatat ctatctggtt ttgagtgcac aaaaggaaga
    10321 ccacaggatg ggaaaatgca ctcaatgggg actatattta aagtaggcgg aatagatgaa
    10381 aaaaaattaa cagaagaagt aataaaagat gcgagcgaaa agttaaaaat tttgagagaa
    10441 aaaggtgaat taaaaatttg gaacactgga cttggtggaa aaggaagcac aataagaaag
    10501 ggagaaaggt catttaatat tacaagattt tctggagatg gaacaaatat aaatgatcta
    10561 acaaaaggtg aaatagaatt aagagaacaa acatttaaac tttttgaatt tctaaagaaa
    10621 aatgtttctg gctttgaaaa tgcctatata tctgctctgc ctgccaatat aggaataaga
    10681 gagacaagac agataaaagg gctttatact ttgatagacg aggacattat ttcagggaaa
    10741 aaagcacaag atagtattgc aagaggaaca tatatgattg atattcattg cccattagga
    10801 agaacaaaaa ataatgtcca tctctgtgta aaagaatgtt ctacaaaaga gtactgtata
    10861 atgctcgata aatatatgga ttatttacct tcaaaagaaa aactttatcc acctatcaat
    10921 gattactatt ctataccttc aagatgtctt atacctcttg gagcaaaaaa tcttgttgta
    10981 ggtggaagat gtatttcagc agagcataaa gcaatggcat caataagagt tatggcaatc
    11041 tgcatggcaa caggtcaagc agcagggata attgcctcat tatcttcaaa aaataatact
    11101 cccgtaaata aagtaaatat aaaagaggtt caaaaaatca taactcagca ggaaggaata
    11161 tattaaaaat gataaaattg agtgatacaa aagaaggaat atataaaatt gtaaatattg
    11221 aaaaatgtgg gaagggaaga ttaaaaaaat tatataattt gggtattttt gttggagatg
    11281 ttattgaagt tataaaacca gggccaggtc ctgtaattat taaaaaagga aatacaagaa
    11341 taggtattgg taatggaata gcaaacgata taataattga accaattgac taaaaagtag
    11401 taagtagcaa gatggaatga aagacacacc tcttattaaa tatttacgat ggaagaaaaa
    11461 acaattgcaa taagtggaaa tcctaataca ggaaaaacta ctatttttaa tacactaaca
    11521 gggttaagac aggaaattgg aaattggccc ggggttacag ttgaaaaaaa agaaggttat
    11581 tttatataca aaaatataaa atttaatgtt gttgacctac ctggaacata tacactgata
    11641 gaagaaagcg aagaccagaa aatagcaatt gactttctga caaatgaaaa agtagatact
    11701 gtcattttaa ctgctaacgc ctcaaatctt gatagaagtt tataccttct tattttgctc
    11761 tctgaattaa atcaaaatat tattgttgtt cttaatatga ttgatattgc agaaaaagag
    11821 ggtattaaat atgatataga aaaacttgag aagatatttg gaatcccttt tgttgaaaca
    11881 atagcaaata agggatatgg aattgaaaac cttaaagaaa aaatatatga aaaaacaagg
    11941 gataaaaggg aaaaatattt ttttaaaata aattatggag aagaaataga aaattatgta
    12001 tcaaaaattg aagggaaact tcaaaatatc tctttgccct acccttctaa atttatatct
    12061 ttaaggttat tagaaaaaga cgaaagaata agagaactga taaaaaataa agtaaatata
    12121 aatgaaattg atgaaattat tggccaatta gaaaaaaaga ttcccaatat agaagttatt
    12181 ttaatagaaa aaagatacgg aattatcaat ggaatggtta aagaatgcct tttatcaaga
    12241 aagaaagtag aaaaaagaat tgaaattaca aattatttag atagagttct tacaaacaat
    12301 tttttaggtc ctttaatttt tcttttgata atgtttataa tatttcagat tgtttttaaa
    12361 tggggagcac cttttcagga acttttagac aaattttttc atttttcagg agaaaaaata
    12421 tctttatttt tacactcaat aaacgccccc ttatttctta tttcttttat tgaagaagga
    12481 ataatctctg gaattggttc tgttgttatg tttctaccaa atatttttct tttcttttta
    12541 ctcttttcta ttttagaaga gacgggctac atggcaaggg ctgcttttac aacagataga
    12601 ttgatgcata aaatgggact tcatggaaaa agtgctattc cactgatact tgggtttgga
    12661 tgtaatgttc ctgcaataat gtcaacaaga acattagaaa caaaaaaaga taaaatttta
    12721 accatccttg ttataccatt tatgagttgt tctgcccgac ttcctgttta tattcttttc
    12781 acaggtattt tcttcaaaag tcatcagtca ttggtaattt tttctattta cttaattgga
    12841 attttgttgg gaattctaat ggcacgaatt ttaaaattta tttttttcaa agaagaatca
    12901 ataccaatta taatagaact tcctccttat gaaattccat actggaaaaa tgtaataatt
    12961 gagggatggg aaagaacaaa agtattttta aaaagagcag gaacaattat atttgtagga
    13021 gtgttgattt tatggttttt gagttatttt cctgacccat ctaattttgg taaagaaaca
    13081 agtttaattg gaagaatagg taattttttt gctcctattt taaaaccagc agggtttggg
    13141 ttctggcagg catcagttgc tttaattttt ggaatatatg caaaagaact tgttgttgga
    13201 tgttttggaa ctttatttgg agaaaacaac ctttctacaa ttctccccta ttattttaca
    13261 cccgcatctg cctattcttt tatgcttatg acattattat atatcccatg tcttgcaaca
    13321 attgctatta taaaaagaga aattggaata aaatgggcat tattttctgc tatattttca
    13381 atttttcttg gctacctcat ctcaactctc ttctaccaac tcaccctcct cttctaattc
    13441 cctaatttat acaaaatttc attaatagta aataaaattc tgtgtaagtc aaaacccaaa
    13501 taagattaca ttttgatatt aacaattctt aatagcatat tttacttaca attatatttt
    13561 tcaataaaat atttaaatga cttatttttc tccattgcca cttctgtaga aaaatgtttc
    13621 aaaatctcta tttttaaatc cttgaggtcg gattctttac caaccaagga tttaaatttt
    13681 cccttatcaa cattatcagt tgtctccaaa gaagaaatct tgagtttttt agcatttgca
    13741 ttatctaacc ctgcaagata ccaactttct attttcttta ttaccacaat tattctatct
    13801 aaatcaacat aactaaattt ttcccatatt tgttgttttt tggcagtcac acacggtgaa
    13861 tcgttaatat cgactacaaa gatataatct gtcataggta tttttttgat gctaagaatt
    13921 atgtttttaa ctccctcttt cgttttctct gcatatttca atataataat ttcataatat
    13981 ctatttttaa attttggctt tactattttg tcaaaaaatc gctcatcatc gggcccttct
    14041 acaagaaaat ataatctatc tttcatatat ttgacccaag taaattttgt acataaagtt
    14101 cttcaatacc taattcattt tctaaaaata ttttaacatc ttctttctct tgtggtttag
    14161 aaataaccga aaaaccattt ttgtcgcggg aaatgagtag aatattttct aaacctgcat
    14221 gttttactat ctcagggttg tgagtagtaa caattacctg tgttgttaca tctttcatca
    14281 tctctaccac ttttgaaata agataaggat gtatatttct ttctggctct tcaattatag
    14341 ttattggttt tttttcaaaa tataaagcaa taattaaagc aattatattt actgttccat
    14401 cagaaattaa agatgcgggt aaatattcct tcttactata tgtttccttt aacttaaaaa
    14461 ataaagattt atctacaaat ttttctacat ttaaattatc tacaaagggt aaaaggtctt
    14521 gtactaaatt aaaaaatttt cttttatttt ccttgttttc tataatattt ttgagaataa
    14581 tggctaaatt acttccatct tcttccaatt ctgcttttcc agttataggc gtagctcttt
    14641 taataatttt agggtcaaaa tcatatattg agatatcact aaaaatcggc ggcataataa
    14701 aagacgtaac tctttccaaa agaagcgatt tttgtttatt ttgttcaatt gttaaaaaag
    14761 ggggaattat atcatccgtt gatatttcat ttggcaattt tgatggaaca acattaattt
    14821 tcccattgat cttagaaata gtaatttctc caatctcttt ctttttttct ttaccgattc
    14881 tttcttgttg tacctcaatg ttaaattttt gtactatttg ctcttcagtt tcaactccta
    14941 atcccctttt tctaaatttt atagtaaagt tataaatcgt ctcgtatacc cccgctctta
    15001 taaatttttt ctctggtttt ttcgccataa aaggtctcat atatacttga tctgaaataa
    15061 cttctaatga aaaattatct gatgagccaa tattcatatt cctgagatat tcaatacctc
    15121 cctgcataga tatggcatta tccaaaccaa aattttttat atctttgaga aatctaaaaa
    15181 tttgtataaa atttgattta ccagaggcat tagcacctat taacacatta aatttaccga
    15241 gatttatttc catatccctg aaacttttaa aatttgataa tttaattttc tttatggcca
    15301 tatttttacc tccttagtaa aatggcagat aaattcgtat atgtcaaata ttcatctatt
    15361 cttcaacaat tctaatttat taaataccat aatatctctc tgccttgtaa aatattattt
    15421 ctccttcaat atatgcttca tttgagattg tatttacaac atttttttca ctttcaaaat
    15481 catttaatgt cttgataatt atatcaaagg gagtaaaagg aaaatgttta tgaagtgcat
    15541 taaatatatc ttgccagatt tttcttttct ggttaatttt tacattattt cttattataa
    15601 ttaaaatatc aaaatcactt tcttcattaa aatcgcctct tgcacgagaa ccaaacagaa
    15661 ttatttcatc aattgtaatg ttagattttg taagcacttc cacaattata tttctaattt
    15721 tttcttccat tgttttttcc atttcttttg ctttcatttc acccatccta aaaaatacta
    15781 ccttttaaaa attagcagtt tttcttcatt aaaattatac aacaaagaaa gaaaaaaggt
    15841 aaatgagaga gagggacata caagggtaga atttttaaat aaaatccacc ttagttcgtt
    15901 gttcttatta agaaaatccc cctcatccct gccctcgagt gtagttctac gggcgagcct
    15961 tctccccttg ggggagaagg aaataaattc cctctttttc cctctcccca taggggagag
    16021 gataggtgag gggagattat tcttaaaatg tttttgagac ataagtcctt gattctcata
    16081 cacttgttaa cttcgtaggt taatagatga gttagagggg ggactgggtt aattcagagg
    16141 taattttgtc tatctcattt tttataactt ctggaagtcc caatatctct gtgttcataa
    16201 aacctcttat tataattgat tgtgcttctt cttctggtat tcctctactt gcaagatatt
    16261 caatttcttc ttttcttatt ttcccaattg ctgcttcatg acttaattct acatcctgaa
    16321 agtcagtctc taattcagga attgcaatta ttttccccct ttcagaaaga acaagtcctt
    16381 tacattctaa atgtcctttg acttgtggtg atattgcttt caagtttcct cttgatatta
    16441 cagttccacc taaggaaatt gctctgctta tcacttctgc tgatgtggct ctttctgcaa
    16501 aaattactct attgccaata tcctgataac aatctggaaa tccaacaact attgagttaa
    16561 atcttactct tgaatttttg cctttgagaa ttgcagttgg atacattact atttttttaa
    16621 ctggtttgaa attcatataa tttgaaataa aaacacctcc ttcttccact actgcaacac
    16681 ttttgggttc aacttcaaca tcttttttcc atgaatggag cattgtaaag tttaaaaaac
    16741 cccctttatg gataaaaaat tctgaaattc caagatggta tgcctcattt gctgccttta
    16801 atgatgtgca accattgata agatataact ctgctccttc ttcaacaatt attatgttat
    16861 gaactttttg tttaaatttt tctgttttta aaattaaaca tgcctgaaca ggaaatagga
    16921 cctttttgcc tttttttact cttataaaat aaccaccctt tgtattttta tcataatgga
    16981 tattaaacat ctcaaatgct ttgccaaaat atttttcttt tacctctgga tatttttcaa
    17041 gtgcattttc tattgaggaa atttctaaat tttcttcttt tatacctgca agtaaaacat
    17101 tattgtcttc ctgaacaaaa ctacctgctc tttctttttc ttctgtatca tatccaactt
    17161 cttcaagttt tttatattct tctttcattt tttttccttt aatatccctc aaatctgctt
    17221 atatttcaca gagataatag gtttcaataa aatcccccct catccctcct tctcccccaa
    17281 gggagaagga ataaaaaccc ccctcacccc cctttctaaa aggggaaact tttgggtaag
    17341 agatatctct ttttaagtgg ggaactttag caccaatttt ttcattttgc cttcctcatt
    17401 ttgcaattcc taaaatcccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
    17521 aaagggggaa atttacactt ttcactaata aggcctgtcc gtcgcaattt ctggcggaga
    17581 aggtagagat ggatttctct ttgtgttttt ttgcctttta gtcaatatag ttaacaaata
    17641 attatttact ggtcctcgca tttacatttt ccgcattttt catatccatt ttttcttata
    17701 tcctgaaata tcttttctgg ttcgtctgaa caacagagtt ttccattcat aagaacacat
    17761 cctctttttg cagaaatata ttgtagaata taaccagtat gagtaattat taatgcagaa
    17821 cttccatttt ttttaaaaaa ttcatttaga actttaccca ttattgagat attttcaata
    17881 tcaacacctg attctggctc atcaagtaaa agaagttttg gcttttgcaa tagaatttga
    17941 aaaagttcag accttttttt ctctccacct gaaaaatcaa catttacatc tctgtcaaga
    18001 aactctttta aatttaattt ttctgcataa tcaaatataa catcatcatt ttcttcaaga
    18061 tatttcgcta tttgataaag ttttactcca cttatttcag gcggttgttg atacattacc
    18121 cctattccca tttttgctct ttcacttact gttaaatttt ttaaactttt tccatcaaat
    18181 aatatatctc cctcttttat tttgtaatca gaaacaccca taattgattt tataagtgtt
    18241 gattttccag agccatttgg tccaaacata ataacaatgt ctccatcttt tattgtaaga
    18301 gaaagttctt ttaaaatctc atctccctct tctgaagtaa caataatttt atcaacttta
    18361 agcattttta atcacctttt taactctctc aaggaaagta ggaaataatt tttctaccag
    18421 taaaataaat ttctgcattc ttctccatct tatatctaaa tattcatgaa caactatatt
    18481 acgatattgt gctaatgcag aaaattcttc agcttcttca ggagaaaaac ccaaaactgt
    18541 acaaaagacc tttaatgttt ctttataact ttgaggtatt gtttttttct ctgaggcaag
    18601 aattattttt gaaatatcaa gacaacccat tacaagattt tcaatccatc tctccaatgc
    18661 tcttctttta atactattct ggttatattc ctgccatgta atgtttttca taatttctat
    18721 atcagtaaac tcattttcaa gaaatctcaa atactttaaa atttctgcct tattttcagg
    18781 agaaattgat tttgcttttt cagatatcca gtaatactca tctacaaatt tccaccaatc
    18841 cattgcctca taactaactt tacataacaa attcaagtaa agttttttat cagatatttt
    18901 taatggtaaa ccatccctta aaattttata aacaagatct ggacttgctc tatttaaaac
    18961 aacaaggtca atattatctg tctgtaaaat ttctacaagt tctccccata ttttttcttc
    19021 ttctggatat tcaaaatttg cctctaattc catatattct actggtttaa aatatattcc
    19081 aatgtcccaa tcagaaatat ttctttgttg tccagttgct cttgaaccaa aaagaaatgc
    19141 cataacaatc tgtggttttg tctcaaaaaa ttttttaaga atttttaatt cattatccat
    19201 ttttattcct cttttgttca attattttac cctaaatcat aacaaaagga taatgttaaa
    19261 taaaaaatca ggaaggtcaa aaatacttaa aataaaccaa ctattttacc atctttatca
    19321 atatcaactt tctcacctgc tggatgagaa ggaagccctg gcatggttct catttcacca
    19381 cataaaggat aaataaatcc tgctcctgct gagaccctta tatctcttat tggcaaagta
    19441 aaaccatctg gttttccttt taaatttggg tcgtgggata aagaaagatg tgtttttgcc
    19501 atacaaattg gcaaattccc aaatccccat tttgtgtatg tctttatttt cttttctgct
    19561 aatggtgaat attctatttt accagcacca taaatttttg ttgctattgt ttcaattttc
    19621 ttttttatat cccagttatc ttcatataaa aacttaaaat ttttctcctt ctctgatgct
    19681 tttataactt tttctgctaa accaattcca ccctcccctc ctttttccca tacctcactt
    19741 acttctacat catctgcacc atattttaat gataactcct ttaatgtttc tatttctttt
    19801 tctgtgtcag ttttaaattt atttattgca acaacaacag gaattccaaa atatttagca
    19861 ttttcaatat gtttttggag attacaacta ccctctttta gtgcttttat attttcttca
    19921 ataagtcctt tatcaagtgg ttttcccggg acgactttaa atttacctga atgcattttt
    19981 aaagccctaa ctgtacaaac aataacaaca caatcgggtc ttagtccact atatctacat
    20041 ttaatatcca taaatttttc cattccacaa tcagcaccaa atccactttc tgtaacaaca
    20101 taatcagata gtttcaatgc aataaggtca gcaattattg aactatttcc atgtgcaatg
    20161 tttgcaaatg gtcctgcatg aacaaaaaca ggtgtatgtt ctattgtctg catcaaattt
    20221 ggttttatag catctttcaa tagaactgcc attgctcctg cacattttac atcttctgct
    20281 gtaactggtt tcccatcttt tgtctgtgca actataattt ttcctaattt ttttctaagg
    20341 tcaaaaatat ctcttgttaa ggcgataatt gccataactt ctgatgcaac tgttatatca
    20401 aagtttgtct ctcttggaat tccgttttct tctccaccaa gtccaataac tatatgtctc
    20461 aaaaacctat ctgaaacatc aacaactctt tgccagttta ttgtcaatgg gtctatatta
    20521 aaaggatttc ctttaaaaac agaattatct acaaatgctg caagtaaatt atgagcaata
    20581 ccaactgcat gaacatctcc tgttaaatgt aaattgaagt catccatagg aattacctgt
    20641 gaatatccac ctcctgctgc tccaccttta attccaaaaa caggtccaag agacggttgc
    20701 cttatgcagg taaaaacttt tttattcagt tttcctattg cctgagaaag tcctattgtt
    20761 gtcacagttt taccttcacc aagaggagtt ggagttattg cagttatatc tatatatttc
    20821 ccatctggtc tatcttttat tttctcaagt atatcaagag aaacctttgc tttgtaatta
    20881 ccatataatt ctaaaaactt attatcaatc cctattttct ctgcaatttc tccaatatgt
    20941 tttatttctg ctttttgtgc aatttctaaa tcacttaaca tttttcttct cccatgttaa
    21001 aaatactgtt aaaatttctt tttttctcct ctcttcccat tgtgcttgga aaaatttaca
    21061 aaatccttta tatcttctgt tgtgttatct gaaacttcca aaatcccctc tacccccctt
    21121 taataaaggg ggaattgggg gatttccatc tcccctgcgg ggagaggctc atctatgtac
    21181 tacgtccgag gggaaaggat gaggggtgcc tttctctcag aaatctctac ttaccttgtt
    21241 ttttcaaaaa accggctctc acctcgaatc atactctttt atcttaacct ttttcaaagg
    21301 ggaaatatta ttttctttgt gtcttatcta accagtgtaa gttaattctt acatattaaa
    21361 tttcttggta aaaaacaatt tcaggtgttt ttaataaacc agaaggaaca atttgataac
    21421 cgttataata ttctttttca ccaggtgtaa attgtcctgg tccagtccat tctgaccatt
    21481 tttcaaacca gtggaaagga ccatgagaat ttcttctact accaattact tctattccaa
    21541 gtattatttc ttcatcattt tttgataaaa attctgtaat ttctacctca tttggttgcc
    21601 atgcaattat ccctgcctgt tgcccattta caaaaatcct taaagcagtt cctctatatt
    21661 caggtattct tacaaaaatt cttttattat cattttctat ttttaattta gtcatataag
    21721 ttaaatttcc tgaataaaaa ggtatccctt tttctgtcca atcaccaatt tcaatttctt
    21781 ctggtttatt aacaatcctt aaatcattat ttttttctat tttcactcca aattcccctt
    21841 ttatataaat tatttctaac ccaggatgat tttcattata ctttgtttgt aaaataattt
    21901 cattttttcc caattttaaa actgatgggt caaaatttat tttccttaat gatttatcca
    21961 cccaccaacc actttccatc tcttcaggaa gcatgtatcc atttatataa attttataaa
    22021 gttctggctt ttcaatacat atacataatt ctcctgatgg aattttatca acaaaaaatt
    22081 catattttaa atggatatcc acacttttag gatttttatt tattttcctt acccatggtt
    22141 gaaccattgc tccccctctt tctttaatcc ctaaatatct tctcactttc ctatcaactc
    22201 ttaatatctc atctttttca ttccatcttt cttgcccaat tttataatat ggcatatcca
    22261 gtacaagaac attatcttct gataatttta tttcccattt gtccttttta atttcttttc
    22321 tttcaatttc ttgtaatttt tccttttttt tacattcaat tgatatttct tttttgggta
    22381 ttaaaaataa tttactgcca agaggagcaa aattcgtttt tatttcatat ccattttctg
    22441 ttttatttat atcgcattta taaacatttc cattttcctg gtcaaattct aatggctggt
    22501 acttaaattc aggaaatcct tttattatta cattatcaaa tttcatattt ctttctctaa
    22561 caggtggaaa tttctctttt ttccattcat ctgatgtatt acataaaaat aaataataaa
    22621 aatcatcatc ttctcttaaa agataaagaa ttggctgtat ttcttttcct ttttcatcta
    22681 agatacttat tcttctggct tttttttcaa tttttttcag cccatcttct atattttcac
    22741 ctatatcaaa aatagatgaa aaatcaaaat ttgccttctc tccatcaata tattcaggaa
    22801 aattttttat aaatataact tcgccacctt cttttttaaa ttcttttaga agttttaatg
    22861 ttgaatttct tattgtatat aaaggtggaa ctaaaactgc cttatactct gccttattaa
    22921 cttttatttt tgtctcttta ccctttttta cttttgccca tcttgataaa atttcttcat
    22981 ctccataatc aaaatctaca tgtaaagata aaagtgactc acataatttc tcaaacattt
    23041 tgtcatattc aactacttcc tctgcctttt ctaaattttt ttcaattaaa tttttgtttc
    23101 tatatattgc ccacatactt tctattggat gaagaacaag caggtctctt acttcatttc
    23161 catttgtcat aataaaattt atccttgcaa aataatcttc aacctttttg taaagattcc
    23221 accatggtga ctgataaaat atacttgctg gataatccct ttttgcttcc cctaacattg
    23281 tgtaccatga aagatgttga catcttaaat ttatcccaag tgcaacctgc caatcaccta
    23341 atgccttatg acctaaaaat ggaaaatccc atccagtgca tccatatgtc tcagtcaatc
    23401 gccatttcct tccaaattgt cttgcaacag aacttacctg ttttgctgta ttggaaaatt
    23461 ttctgtattc tgttaataaa tccattccag gtgcttgcat atattcataa aacctttgac
    23521 aacttccaac aacagatgtt tgtgatatta atgtgtcttc tgcgagaaca tgtcctgtaa
    23581 ataacaaatt gttttcctca caccacttac ctatttgttt tgaaaatgag gtagtaaaaa
    23641 gaaatgttat acaatcaaaa taattgtatc ggacttttga aattttttct ccaacaacat
    23701 taaaataaat ttctggtaaa aaatttaaaa tgtcataccc atattttttc ttaaagacat
    23761 ttgctaattt ttcagtccat ggtgtatcta tcccattatc ctctaagaaa aaagaagtcc
    23821 cataattcgg ttcatctgta aaaattcctg ggattgttga accaaaatat ttgccacaat
    23881 actttttata ttgttgatgt gttactttta taaattcctt tactgcatct ggatttaatg
    23941 tatctaaata tgtctgtcca ttataccaat cactttcttt ctggattttt atagaaaaaa
    24001 ttaaaatttt ttcacttttt tttaatttta tttcttctcc tttttttact tttctatatt
    24061 cctttatata tggtccttca aagtcagcaa taaaaattcc tttcacatct tttatatttt
    24121 ttatttcatt tacatcatct aaaattttca tatctaaata tctcattcta tattttttat
    24181 tttttgtaac tattccacca cctgctccac ttggccatct atcttcatca taaagaaaag
    24241 caagcatatt taatttttcc ccttcatctg cacatgcatt tatataacta aaccattctt
    24301 ctgataaata tggggtatca agtcctactc ttgaatgcat aaaaaatcca cctaatccca
    24361 tctgtttcat cattcttatc tgccatctta actccttttc ttctaatttt ccattccatg
    24421 cccaaaaagg cgctcctcta taataacagc ctggctcctt aaacccctca attatctttt
    24481 ttatttccat tttctctcct tttttataaa aattcttctt atcatttttg ggtgaaatat
    24541 cactataaga gtaccaccta ttatatttcc taatgtaact ggtataagat ttttccagat
    24601 taacaaaaaa ttttttaaaa actgcccatt taccatcaaa cccaaaggta aaaaatacat
    24661 atttgctatt gaatgctcat atccagatac cacaaaaacc attattggaa aatatattcc
    24721 aaatatttta ccttcaattg tcaaagatga aatacacatt attacagcaa gacaaacaag
    24781 catattacat aatatccctc tacaaaatga ctggaataaa ggtaaattta tttttaaaat
    24841 tgcaatatca actgcgacat ctcctattgg tgttagccct gataaaaaac ttccaagtac
    24901 tcctgatttt agaacaatat aggatagaaa aacagaccca ataaaatttc caatatagac
    24961 aacaacccaa tttctaatta attttaataa attatatttt ctatcaatca atccaagaca
    25021 cattaggata ttacctgtaa aaagttctgc tcccggaatt aaaacaagca taagtccaac
    25081 actaaaaaca ctacctgcaa gaaattttaa tataacaggg tcaacatttt tgccacttaa
    25141 aattgttaaa gatgcatgtg caccaaaacc aatataaatt ccagcaagaa ttccaaaaaa
    25201 taaaagttca aaaattgttg attctgcctt ttttataaca attttctcaa gttcattaga
    25261 aatttctaat ggtgaaagaa ataatttcat aaaacccccg agtgttcaag aacttctgga
    25321 acatatttgt cccatccaag tggcattatg tgaattccat tacacatatt tttaacttct
    25381 tttattattt ctactgctat tttaatactt acttttgtcc tttcttcttt ttttgcctct
    25441 gctatcattt ttatatattt ttcaggaata cttacacctg caactttttc attcatatat
    25501 tttgccatac ctgctgattt aagaagaaca attccgcaaa ggactggaac attataattt
    25561 tcagtcattt ttataaattt ttcaaaaata tttatatcat agacggcctg tgtctgaaaa
    25621 aactttgctc ctgc
//
LOCUS       DAOOVW010000025        25344 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_1321_length_25344_cov_7.510657, whole genome shotgun sequence.
ACCESSION   DAOOVW010000025 DAOOVW010000000
VERSION     DAOOVW010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 25344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25344
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1321_length_25344_cov_7.510657"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PLW95_04705"
     CDS             complement(<1..261)
                     /locus_tag="PLW95_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21964.1"
                     /translation="MLIDRKVTGEYLGVITPEGLKEPGYRINRPTTCKEYGASWFLIK
                     ENEEVIDKLKNTFPIFWKELFILSVCRLLYQSPLKHMELHYQD"
     gene            962..2104
                     /gene="nusA"
                     /locus_tag="PLW95_04710"
     CDS             962..2104
                     /gene="nusA"
                     /locus_tag="PLW95_04710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOV21965.1"
                     /translation="MKTDLAFLLEYWEKEKGIEKKYLIESLEKGLLSVYRKKAGLPSN
                     VDIKIDPETGEIKFLDNQGEEIAPPSFPWERIAAQTAKQVILKKLKEAEKITIYNEFK
                     QLEGEIISGMVERFEDGHVVISIGKAEGLLPQHHKLPNDHFKIGYPIKAYLLEVRQPN
                     KGIFQLIVSRTHPNFILKLLEKEIPEIKEGIIIVKGIARFPGDLTKIAVYSKDEKIDP
                     VGTCIGNKSLRIKNIVKELNGEKIEIISWDRDITKFIENSLSPAKCEKVILKKGKREA
                     IAIVDDSQLFLAIGKRGQNVRLASKLTNWDIRVYKKSEFAENVKPAISIIDELTEEMA
                     EILAKNGYNSLKDLSEADVSEIANLLKIPEEQAQQIITKAGNYIKK"
     gene            2101..4044
                     /gene="infB"
                     /locus_tag="PLW95_04715"
     CDS             2101..4044
                     /gene="infB"
                     /locus_tag="PLW95_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOV21966.1"
                     /translation="MRIYEIAKKLGVHSKKVIEILETLEIKNKTHLSALSEEEYKKFK
                     EYLSREEKITKPTTVEEKVKKVIKVSENETVGEISKKFNLSPSKVIKDLMEMGIIANI
                     NQSISLDTIKKLCDKEGYICEVIKGEEEKEKEKEKKISKNLIPRGPVVTVMGHVDHGK
                     TTILDSIRKTKVVEQEAGQITQKIGAYKIVVNNHPIVFIDTPGHEAFTAMRALGAKVT
                     DIVVLVVAADEGIKPQTIEALNHAKSANVPIIVAINKIDKPNANIDRVKQQFSEIGVI
                     PDDWGGDTIFVPVSGYTGQGINDLLEMIILVGEMLELKADPTGKGEGTVIESKLDQKM
                     GPLISIIVENGTLNISDSFVVGNTWGKVKAMFDDKGNRLTKAGPTTPVEILGSHNTVK
                     PGEKFLVVDSEKEGRRIAEEKNKKTIIQPVKKITLEDLYKEIEKGIIKELKIILKCDT
                     SGSIDAIKEAISKVPQNEVEINILHSGTGQISESDVLLADASNGIVIGFNVAIEPKAK
                     DLAKKEHVEVKTYNIIYDLIDDLSKSIEGMLIPETKETLTGQAIVKKVFKVGKNLAVA
                     GCLVVDGKVIINSKAKVVRDGQIIYEGSLVSLKRFKESVREVPKNTECGIIVSDFTNF
                     KEGDIIQSYIEEEVPKNLTKERK"
     gene            4041..4379
                     /locus_tag="PLW95_04720"
     CDS             4041..4379
                     /locus_tag="PLW95_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HOV21967.1"
                     /translation="MSECIFCQIIKGQIPAEKVYEDDDCIGIKDVNPQAPVHILVIPK
                     KHIDKLYNLEDEKLLGKMLIVCSKVAEMFSIKENGYRIVINTNKFAGQSVEHLHYHLM
                     GGRIMNWPPG"
     gene            4548..5369
                     /gene="kdsA"
                     /locus_tag="PLW95_04725"
     CDS             4548..5369
                     /gene="kdsA"
                     /locus_tag="PLW95_04725"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010197315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOV21968.1"
                     /translation="MTKEIRIKNVKIGGENIFCLIGGPCIIENEEIVFQTAETLKNIC
                     EKHQIPFIFKASFDKANRTSINSYRGPGIEKGLEILNNVKEKLNIPITTDVHCIEQIE
                     KVAKVVNLLQIPAFLSRQTDLIIEAGKTGKPVNIKKGQFLAPWDIKNIIEKFESTGNK
                     NLLITERGVCFGYNNLVVDFTGIPIMRCLGYPVIFDATHSVQKPGGMGKKSGGNRELV
                     PYLAKAAIAVGVDGIFMEIHPEPEKALSDGPNMLKLEDVEELLIILKNIYYAIKK"
     gene            5467..5610
                     /locus_tag="PLW95_04730"
     CDS             5467..5610
                     /locus_tag="PLW95_04730"
                     /inference="COORDINATES: protein motif:HMM:NF021558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2281 domain-containing protein"
                     /protein_id="HOV21969.1"
                     /translation="MSELKIEERIKKLPLYLQKEVEDFISFLLEKHQKISGKKSLRTG
                     LMH"
     gene            5682..5972
                     /locus_tag="PLW95_04735"
     CDS             5682..5972
                     /locus_tag="PLW95_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21970.1"
                     /translation="MYLVDTNVWLERLLDQKELPAVKNFLNKISSELLFIMDFYFHSI
                     SLIMIKLGQRIGKFNLDFDDTYQYVSAEKYGLTLVSFDSDFDSTKPEEIINI"
     gene            6035..6223
                     /locus_tag="PLW95_04740"
     CDS             6035..6223
                     /locus_tag="PLW95_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21971.1"
                     /translation="MKGTETQSENQKIKFPLTSILSPVGRGRKREGYFLLPWRRKLAR
                     RATGEGGNEGKLFSLLYT"
     gene            6259..7242
                     /locus_tag="PLW95_04745"
     CDS             6259..7242
                     /locus_tag="PLW95_04745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOV21972.1"
                     /translation="MSKKEIIEFGKRIIEIECQNLERVKENLGDSFAEAVETLKSLEG
                     KVVITGMGKSGIIGRKIVGTFASIGISSIFLHPGDAVHGDLGVVSKNDVVLLISNSGE
                     TDELIRIVPSIKKIGAKIISITATTKSTLGQYSDIVIETGEITEADNFGIIPSSSTTT
                     ALVIGDALSLTIMNIKNIKKQDFAFFHPAGNLGKRLMLRVKDIMQTGENIPIVDENRT
                     LIDGIKEINRKNLGFTLAINKNNCLTGIITDGDIRRLLAKDIDITKLKIKEVMIKNPK
                     TIDEDILAVKAMEIMEKYEITALPIVDENKKIKGVVHLHDLLGKKEFGIEY"
     gene            7232..7738
                     /locus_tag="PLW95_04750"
     CDS             7232..7738
                     /locus_tag="PLW95_04750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOV21973.1"
                     /translation="MSIKNIKIFISDVDGVMTDGGLFYINDCLYRKFNVKDGIGIKML
                     KIVGIEIAILSSNVSVQTKNRFLSLGVNLYFEGIKEKDKFIEGFLKENNLQWDNVCYM
                     GDDLQDIKPIRKASFSICPSDAVEEIKEIVNYVCKRKGGEGAFREGVEKLTKKIGKWE
                     KIKKEYLL"
     gene            7705..8268
                     /locus_tag="PLW95_04755"
     CDS             7705..8268
                     /locus_tag="PLW95_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21974.1"
                     /translation="MGKNKKRIFIITVLAFFCILILPGCNRKQVENKKIDESQLKKFS
                     FKQFSENFNLTIIGEGAEINEKDIKVNSPSFYIKGETDIIEIKTGKEGTAEITIQPEN
                     KQIQNVVFTGNIKITQKDKVSGKIIMEAECGKLTYNDMAKEMIMEISPVIKRENNIFS
                     GEKIYYFLEKNTLQIKGNVNVKIIPEK"
     gene            8243..9217
                     /locus_tag="PLW95_04760"
     CDS             8243..9217
                     /locus_tag="PLW95_04760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOV21975.1"
                     /translation="MLKLFLKNSFIFWFLISGIFCSAENITIGKYNNCSSDEVKKAPY
                     SGNIYIKESQNKYIILDIDEYLSGVLSGEIGEDFPYEALKAQAVVSRTYLLFTSKKNK
                     QNGLSYDIENSIYNQVYKVCNSQKIRNAVDETKDEILTFNGEIVEVFFHACCGGKTTS
                     PSSVWGGNYNGSINGIIDDYCKGTPYYSWEKKYSSDYLSDIFGLYNIDKIEIVEKDAS
                     GRVKNLNLITKGGRVIQLSGHKFRLQINSATNVYFENPLLLPSTMFDVEKKDNDFIFR
                     GYGYGHGVGLCQWGAKKMAESGKNYREILKFFFSDMEIISLGGGENEN"
     gene            9207..9500
                     /locus_tag="PLW95_04765"
     CDS             9207..9500
                     /locus_tag="PLW95_04765"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HOV21976.1"
                     /translation="MKINIHSNIELDDKFQEYLNKKVEKLEKFIFDEGNADIYIKKDG
                     PFYISEIDIKTKKHTIFLKEKEKNISKCIDFLIDKTKRKLAQLHDIVIDRSKK"
     gene            9536..10009
                     /locus_tag="PLW95_04770"
     CDS             9536..10009
                     /locus_tag="PLW95_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOV21977.1"
                     /translation="MKITDFLDKRCVIIGMRSRTKKEAIKELLTKLKENGFIKDEKEI
                     LETVMEREKLGSTGIGQGIAVPHAKSDQIENLVAALGISKTGIDFNSLDGEQVNIVFL
                     VLAPTKSVGLHLKALAKIARLLKDRVFRNALKDAQSPEEIIDLIKEDEQKLNNVG"
     gene            10054..11763
                     /gene="ptsP"
                     /locus_tag="PLW95_04775"
     CDS             10054..11763
                     /gene="ptsP"
                     /locus_tag="PLW95_04775"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOV21978.1"
                     /translation="MEKLKGIGVFSDIVFGKGFVWKNFFLLSQEYKIPKKDIKEEINK
                     LDEAIKKTIEEITALKKNLEESIGKEYADIFNFHLSILQDKNLRDETVKIIKEEQLNV
                     ESSLKKVIFKLGEIFGKSEKDFLKDRRRDILDVVEKIVSNLKDIPSMKVIGEGEIIVA
                     DDLSPSQIVALNRKLVKGIVTDIGSETSHIAIMVRALEIPSVIGVGEATKVIKTGDGL
                     IVDGEEGIVIVNPTNQVINEYKKKKEELKKKRKKISLLKNLKCKTKDRKEIKLFANIA
                     FPEEIIVAEKNGYDGIGLYRTEYLYINRKNLPDEEEQFYAYKNIAERVKNKPVIIRTI
                     DIGGDKILPGIFEKKETNPFLGWRGIRFCLDRRDIFETQIKAILRASVYGEIKIMFPM
                     VATLEEVIEGKKVIEDVKKKLKRENKKFKEIELGTMIEIPSAALISDKLAKEVDFFSI
                     GSNDLIQFTLAVDRLNEKITHLYQPCHPSVLKMVKLTIENAEKNKIQVGICGEMASIP
                     DIACLLVGMGIDELSMAPISIPLVKEKLINKKYQDLKEISEEVFKFNTNNEIINFLKE
                     ELK"
     gene            11779..12813
                     /locus_tag="PLW95_04780"
     CDS             11779..12813
                     /locus_tag="PLW95_04780"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF006426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HOV21979.1"
                     /translation="MIEKEIKKIDKSNMRDLLIKFGEHCISGYSLLPSFIPENIKFNK
                     IIYCGMGGSAISGDILKFIVEKHSHIPFAISRDYDIPSFADNETIVFITSYSGNTEET
                     ISTFTQAIEKKANTFIISSNGELEKLSVEKNLPFIKIPSGMPPRCAFGYLFFASYRIL
                     QQFGLLPEIEKKLFQKIDEIVKSFSETENNYAITIAKKIHNKVPILYSDNFIFGCLLR
                     WKTQIAENSKAFSFINVFPEMNHNEIMSFHFPVWFLKKIICLFFIHNEENNRTKKRFE
                     ITEKIIQGKGIETLKVEGKGNSLIEKMLYFVILGDWVSYYLSLLNKIDPTEIKEIIYL
                     KDQLKGGKFE"
     gene            12806..13945
                     /gene="metK"
                     /locus_tag="PLW95_04785"
     CDS             12806..13945
                     /gene="metK"
                     /locus_tag="PLW95_04785"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017186814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOV21980.1"
                     /translation="MSKTFLFTSESVTEGHPDKICDQVSDTILDEVLSQDKNGRVACE
                     TLTTRGLIFVAGEISTRGYVEIPFLVKNILKDIGYIEPSFGFYYDSIGIITSIQEQSP
                     NIAIGVNKGGAGDQGMMFGYATNETPELMPLPIILAHKLTKRLAEVRKKKIVDYLRPD
                     GKSQVTIKYENEIPKTVECVVLSAQHNPEISHKKIVDDLTETVINDIIPNEIRSKKTK
                     IFINPTGIFSIGGPIADTGVTGRKIIVDTYGGVGSHGGGCFSGKDPTKVDRSASYCAR
                     YLAKNIVASGIAKRCEIQLAYVIGLKQPVSIMVNTYNTGIIPEEKISKIIRENFDLSP
                     AGIIKKFDLLRPIYRKTACYGHFGREEKEFKWEYIDSVDMFKKSA"
     gene            14213..15460
                     /gene="ahcY"
                     /locus_tag="PLW95_04790"
     CDS             14213..15460
                     /gene="ahcY"
                     /locus_tag="PLW95_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOV21981.1"
                     /translation="MEYSIKDIGLSETGEKRVNWAYNEMKVISLLRKEFLGKNAFAGT
                     KIGCCLHITSETANLVINFKKAGGDVYLCASNPLSTQDDVAAYLVKEEKIPVFGKKGE
                     NEKEYYENIKKVMEKNLNLVVDDGGDLITALHRSPDYAKNIIGATEETTTGVIRLKNM
                     AKKGILKFPVIAVNDAKTKHFFDNRYGTGQSTIDGILRSTNILLAGKIAVVCGYGWCG
                     RGIAKRFAGMGAKVIICEVNPLTALEATMDGFFVMPISKAAEIGDIFITATGNSSVIT
                     IKEIMKMKDGAILGNSGHFNVEIDVKELEKIGKKIRIREQLDQYTLPNGKKIYLLGEG
                     RLLNLACAEGHPSSVMDMSFANQFLSHLYLKTHPNLKPDVYDVPEEIDKKIAELKLKS
                     LNIKIDKLTREQKEYLNSWKTGT"
     gene            15474..16073
                     /gene="coaE"
                     /locus_tag="PLW95_04795"
     CDS             15474..16073
                     /gene="coaE"
                     /locus_tag="PLW95_04795"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOV21982.1"
                     /translation="MEKIKRVGVTGIFGSGKSTVTSIFKKYGIEVISCDEIVHFLLEK
                     ENIKKKLIKIFGKEIVKDGKIDRKKISDIIFKNKLKKKELENLIHPLVFKEIDKKIKR
                     DIDKIKKKGIIIVEIPLLFETKSEKKFDFILVVSASPEIIKERLKEKFSSKEVELRWR
                     NQIPLIYKEKNANFVIDNSGTISQTEKIVKEIIKKIISQ"
     gene            16186..17469
                     /gene="rho"
                     /locus_tag="PLW95_04800"
     CDS             16186..17469
                     /gene="rho"
                     /locus_tag="PLW95_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOV21983.1"
                     /translation="MDQKEINVSNLITLTIPKLQKIAKEMGINGISGLKKDELVYKIM
                     ETQINKNGVLFGEGVLEILPDGFGFLRMPSYNYLPGPDDIYVSPSQIKKFGLKTGDNV
                     SGQIRPPKDSEKYFALLKVEMVNGQPTEKLQSRIPFEELTPIHPNKRFILETTSDEIT
                     TRVIDLVTPIGKGQRGLIVSPPRAGKTVILQKIANAIAENNPEVVLIVLLVAERPEEV
                     TEMRRLVKGEVISATFDEAPERHIQVSEIALEKAKRLVEHKNDVVILLDSITRLARAY
                     NVLVPHSGKIMTGGLESTALDKPKRFFGAARNIEEGGSLTIIGTALIDTGSKMDDVIF
                     EEFKGTGNMELNLERKLADRRTFPAIDINRSGTRREELLVDPDELQLMWLLRKMLAPL
                     NEVEAMEKMITMIKNTRSNIELLLSLKSKVKEFVK"
     gene            17600..17842
                     /locus_tag="PLW95_04805"
     CDS             17600..17842
                     /locus_tag="PLW95_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012507096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOV21984.1"
                     /translation="METRIQKWGNSFAIRIPKTIAKEIGIENNSPVEILLKNGKVVIS
                     PKVKPKMNLQQLLSKVNKKNLHNEVDTGSAMGGEVW"
     gene            17836..18183
                     /gene="mazF"
                     /locus_tag="PLW95_04810"
     CDS             17836..18183
                     /gene="mazF"
                     /locus_tag="PLW95_04810"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HOV21985.1"
                     /translation="MVKKKKYIPQRGDVIYISLNPQAGHEQAGRRPAVVISPLAYNNK
                     VGLVILCPITNQIKGYPFEVIIPDGLIITGAILSDQIKSLDWQARKAKFICTLPVETI
                     HEVLEKLSVLLLE"
     gene            18233..18499
                     /locus_tag="PLW95_04815"
     CDS             18233..18499
                     /locus_tag="PLW95_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV21986.1"
                     /translation="MTIIYKNRIEGLSCGCTSEDIATFKVIEDGIECIWCGNKINCNL
                     PVEFVMKEYCENCGKKTTFILDTIQMNFCTKCGKSKQSKDKLKK"
     gene            18779..19291
                     /locus_tag="PLW95_04820"
     CDS             18779..19291
                     /locus_tag="PLW95_04820"
                     /inference="COORDINATES: protein motif:HMM:NF026041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HOV21987.1"
                     /translation="MDLNTLTKILNIEGCKVVEILSLTEEEIHLKIEPYKRKPAICSG
                     CGEEHIQGYHSEKEVIVEDLPISERRVFLHVKKRLYRCSRDNKIYTEKIEWLNKRSRF
                     TYRFAKQVNRLTAITTNQEAGWYLGLGDEVVYRIYKEMLEKQAKEKLIPIPSAIHISV
                     DEVIYKNTGI"
     gene            19480..19908
                     /locus_tag="PLW95_04825"
     CDS             19480..19908
                     /locus_tag="PLW95_04825"
                     /inference="COORDINATES: protein motif:HMM:NF013753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOV21988.1"
                     /translation="MLLKESFLEVYTHERVEEAKECLENWIKQALSSGLDVFIQLGYK
                     CQEKMKYILNWFYKSTGSFGKVSFAISEGFNNKIKRLKSPQDPSGVVLRGSVAKSEDP
                     TGEPVEMAYGYKDINYFRLKIHQSCPRQIRFWRTLRFIKP"
     gene            complement(19996..21087)
                     /gene="lpxK"
                     /locus_tag="PLW95_04830"
     CDS             complement(19996..21087)
                     /gene="lpxK"
                     /locus_tag="PLW95_04830"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOV21989.1"
                     /translation="MTVNSEKLKYHVNLVQLKTSRPFFNFFYRKFLFRKRSVFFKLVS
                     LFFLPISFLYLFVITLKRCFSKKRKYKFFIISVGNIITGGTGKTLLVSEIIKKVISLN
                     YKIAVVSSIFKKEKGESIPYDEVIMIKNNFPSIIISGDKTPSGLEKLEEMGIEIVVID
                     DGFHCQWVEKDVDILMFDSSNPFDNKLLIPSGLLREPVSSIKRADVFIISYPHLVGEK
                     EKYTLLTFLQKQEKPIFFLKIKPVYLSNGKEKVLLEEVKGKKILCFAGIGNPFNFFLT
                     ISQLKPEKQYSVVFPDHFNYKEKDINEIMEFFKKENIDFIVTTEKDYVKVQKFIKGNI
                     PFYFLKIEGEIEDKNNFDKLLCNKINIKI"
     gene            complement(21014..22243)
                     /locus_tag="PLW95_04835"
     CDS             complement(21014..22243)
                     /locus_tag="PLW95_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HOV21990.1"
                     /translation="MYIADFHIHSKYSKATSKNMVIEELTKWAKYKGINILGTGDITH
                     FMWFYELKGKLKQTDRYGIYTFNGVDFILTGEVNNIFEQDDVIKKIHNIIFLPSFEVA
                     EKFNRKIEKYGDLNADGRPILHLSCIDLLNIIKEINEDIFVVPAHIWTPHFSVFGANS
                     GFNSIYDCFGRFTSEIFALETGLSSDPEMNWMVSPLDRFSLISNSDAHSPLKIGRESN
                     VFKDKFNYFELIEILRNKDKDKFLMTIEYFPEEGKYHFDGHRNCNVCFSPEESAVNNN
                     KCPVCGRNLTIGVMHRVYDLADRKRGERPKKYIDFKKLVPLIQIISNVYKKGEDSIEV
                     RNHYIEIIKKTGSEFSVLIQIPENELLNTIDEKIAKGIISVREGNVKINPGYDGEFGK
                     VEIPCESCSIEDQPTLF"
     gene            complement(22257..22739)
                     /locus_tag="PLW95_04840"
     CDS             complement(22257..22739)
                     /locus_tag="PLW95_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017952484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOV21991.1"
                     /translation="MNKEIIWAPWRIGYINKIEKRKCFLCYGYKSKKDKENFVVYRGD
                     VSFIIMNIFPYNNGHLMVAPKRHIKDITSVNQKEEIEIMQLIKKSVSILKKVLNPDGF
                     NIGLNLGKVAGAGLDDHIHFHIVPRWNGDTNFMPVISNTKVIPQSLSELYSVLIKEFK
                     "
     gene            complement(22736..23191)
                     /locus_tag="PLW95_04845"
     CDS             complement(22736..23191)
                     /locus_tag="PLW95_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HOV21992.1"
                     /translation="MIEEFVGVSACLVGENCRYDGRNSLNKKVVNFISKKNLIIFCPE
                     ILGGLPVLREPAEIKYGNGKDVLNKKCRVINKKGEDVTEYYVKGAEITFRILKMYNVN
                     FVILKQLSPACGKGKIYDGSFSKKIIEGNGVTAQFLLDNRIGVITEEEL"
     gene            complement(23188..24636)
                     /locus_tag="PLW95_04850"
     CDS             complement(23188..24636)
                     /locus_tag="PLW95_04850"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOV21993.1"
                     /translation="MVKYKKILKWVRTKMGKTKIQIEKIIEKMRDFAKKKGIDIYLVG
                     GYIRDNLLRKKSVDVDFVMEEDALKIANEFSKMYNLPTPVFYGRFGTAMIEINGIKVE
                     FATARKESYPDDTRKPIVEKCNIYQDLLRRDFTINAIAENLITGEILDPFEGRQDIKR
                     KIIKTPTDPDKTFYDDPLRILRGIRFSTRLKFKIEDKTKESMKKNVYRIKIVSQERIT
                     DEILKIISATKPSDGFYLMDEIGVLEIVLPEVFSLKEKRTDHPCKDLFEHTLKTLDNI
                     SRHTRNVYLRFSALIHDIGKPATLKVENGKVSFHRHEFLGEKMSYKICKRMKISDENA
                     KFIGKLIRFHLRPHLLAKENPTDSALRRFIKEVGKDIKALFTLAKADITSKNEIKVKK
                     AIEKLEKLEERIKEINKKDKLSSFKLAIDGFVIMDILTIKQGKEVGFVKNYLENLVLD
                     GILPNKKTYLKRYLKENKKEILHKIKKELQLK"
     gene            24812..>25344
                     /locus_tag="PLW95_04855"
     CDS             24812..>25344
                     /locus_tag="PLW95_04855"
                     /inference="COORDINATES: protein motif:HMM:NF024908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOV21994.1"
                     /translation="MGYKYAKKFIPLRKGEVVSKGCEIVHLSKKGKERLKWIKYYKSH
                     EENASLVSRYYGISRKTFYKWYGRYKLLGLKGLEDMSKAPINKRGHEITWEEELRIKK
                     LRKQYIRYGKEKLSVLYKKIYGEKISGWKIYQVIRKYNLYWSPVKNEKLRKKRKLAEK
                     KKRITELKNKQLAGFFF"
BASE COUNT        10009 a         3224 c         4161 g         7950 t
ORIGIN      
        1 atcttgataa tgtaattcca tatgttttaa aggtgattga tataataacc tacaaacaga
       61 aagtataaat aattccttcc agaaaattgg aaaagtattt tttagtttat ctattacttc
      121 ttcattctct tttattaaaa accaacttgc tccatactct ttacatgttg ttggtctatt
      181 aattctatat cctggttctt tcaatccttc tggggttatt acccctaaat attctcctgt
      241 taccttcctg tcaataagca ccttaaatgc acccctaatc ggcagttgat actacaccac
      301 ttttttaata atatccctgt gtaaattttg acatcaccta ctaataaaaa tcttgttttt
      361 taaaaaaaca gcagtagaat ttgtggataa tgtgggtaac tttccatgag ttatccaagt
      421 tttcgtggat aatgaaaata actaacttta ttaattaaaa atgctttatt catcctccac
      481 aaaacggcag catccccaaa atttcatttc acctgcctgt ttttcttttt cctcttttct
      541 atactaataa gtgtgtatat taaacctgca aaaatattac ctttcagtag ttatttttgt
      601 tttttaaaaa atatatcaaa acccacacat attaaggacc tgcagaacac ttgacaacaa
      661 cttggggggc ataatgaaaa cagaattaaa atttctcaat taatagtaga tatgttaaag
      721 gttaattgat aaaaaaattt gaactttaac ttgctcactc tgcttttaat agagtacaaa
      781 agtagataaa tattcttatc agaaagacag ggttgagtaa atatttacct tcattaaaaa
      841 tttaaatttc aaacttttca aatcaaaaag cagttttcta tcataatcta attgaaaatt
      901 tttagggttg ttgacaagag aaaagaaatt taatacaatt aaacttggag gttttataaa
      961 aatgaagaca gatttggctt ttttattgga atactgggag aaagaaaaag ggattgagaa
     1021 aaaatattta attgagtcac ttgaaaaagg gcttttatct gtttacagga aaaaagcagg
     1081 actacccagc aatgttgata taaaaataga cccggaaaca ggggagataa aatttttgga
     1141 taaccagggt gaagaaattg cacctccttc ttttccatgg gaaagaattg ctgctcaaac
     1201 agcaaaacag gttattctca aaaaattgaa agaagcagaa aaaattacca tttataatga
     1261 gtttaaacaa ttagaaggag agataataag tggaatggtt gaaagatttg aagatgggca
     1321 tgttgtaatt tccattggaa aagcagaagg actgctacct caacatcata aattaccaaa
     1381 tgaccatttt aaaattggat acccaataaa agcatattta ttagaagtaa ggcaaccaaa
     1441 taaagggata tttcagttaa ttgtttcaag aactcatcca aattttattt tgaaattact
     1501 tgaaaaagaa attcccgaaa ttaaggaagg tataataata gtcaaaggta tagcaagatt
     1561 ccctggtgat ctaacaaaaa tagcagtata ttcaaaagat gaaaaaattg accctgttgg
     1621 aacatgtata ggaaataagt cattaagaat taaaaacata gttaaagaat taaatggaga
     1681 aaagatagaa attatttcat gggatagaga tataactaaa tttattgaaa attcattaag
     1741 ccctgctaaa tgcgaaaaag ttattttaaa aaaaggaaaa agagaagcaa ttgctattgt
     1801 tgacgattcc cagttatttc ttgcaattgg gaaaagagga caaaatgtcc gtcttgcatc
     1861 aaaattaact aattgggata taagagttta taaaaaatct gaattcgcgg aaaatgtaaa
     1921 accagcaata agtataatag atgaacttac agaagaaatg gcagaaattc ttgcaaaaaa
     1981 tgggtataac tcattaaaag acctttcaga ggcagatgtc tctgaaattg ctaatttatt
     2041 gaaaataccc gaagaacaag cacaacaaat tattacaaaa gcagggaatt atataaaaaa
     2101 atgagaatat atgaaattgc taaaaaactt ggtgtacata gtaaaaaagt gattgagatt
     2161 cttgaaacac ttgaaataaa aaataaaaca catttaagtg ctctttcaga agaggaatat
     2221 aaaaaattta aagaatatct ttcccgagag gaaaaaatta ctaaaccaac aaccgttgaa
     2281 gaaaaagtta aaaaagtaat aaaagttagt gaaaatgaaa cagttgggga aataagtaaa
     2341 aaatttaatt tatcaccttc aaaagttatt aaagacctta tggaaatggg aataattgca
     2401 aatataaatc aatccatttc tcttgataca ataaaaaaac tctgtgataa agaaggttat
     2461 atttgcgaag ttataaaagg cgaagaagaa aaagaaaagg aaaaagaaaa aaaaatatca
     2521 aaaaatctta ttccaagagg tccagttgta acagttatgg gacatgttga ccatggaaaa
     2581 acaacaattc ttgattcaat aagaaaaaca aaagtagttg aacaagaggc agggcaaata
     2641 acacagaaaa tcggtgctta taaaatagtt gtgaataacc atccaattgt gtttattgat
     2701 actcctggac atgaagcatt tactgcaatg agagcattag gagcaaaagt tacagatatt
     2761 gtagttcttg tcgttgctgc tgatgaagga attaagccac aaacaattga agcattaaat
     2821 catgcaaaat cagcaaatgt tccaattatt gttgctataa ataaaattga caaaccaaac
     2881 gctaatatag atagagtaaa acagcaattt tctgaaattg gtgttattcc agatgactgg
     2941 ggtggagaca ctatttttgt acctgtttct ggctatacag gacagggtat aaatgacctt
     3001 cttgaaatga taattcttgt tggggaaatg ttagaactta aagcagaccc aacaggaaaa
     3061 ggtgaaggaa ctgtaattga atcaaaactt gaccagaaaa tgggaccttt aatatcaatt
     3121 attgtagaaa atggaacttt aaatatttct gactcatttg ttgttggtaa tacatgggga
     3181 aaagtaaaag caatgtttga tgataaagga aatagattga ccaaagcagg tcctaccact
     3241 cctgttgaaa tattaggttc tcataacact gttaaaccag gagaaaaatt tttagtggta
     3301 gattcagaaa aagaaggcag aagaatagcc gaagagaaaa ataaaaaaac aattattcaa
     3361 ccagtaaaaa agataactct tgaagacctt tataaggaaa ttgaaaaagg aattataaaa
     3421 gaattaaaaa ttattttaaa atgtgatact tctggttcaa ttgatgcaat taaagaagca
     3481 ataagcaagg ttccccaaaa tgaagtagaa ataaatattc tacatagtgg aacaggacaa
     3541 atttctgagt cagatgtttt gcttgcagat gcttctaatg gtatagtaat tggatttaat
     3601 gtagcaattg aaccaaaagc aaaggacctg gcaaaaaaag aacatgttga agtaaaaaca
     3661 tacaatataa tttatgacct tatagatgat ttatcaaaat caattgaagg tatgttaata
     3721 ccagaaacaa aagaaaccct tactgggcag gcaattgtaa aaaaggtttt taaagttgga
     3781 aaaaatttag cagttgcagg atgccttgta gttgatggaa aagtaattat aaattcaaaa
     3841 gcaaaagttg ttagagatgg acagattata tatgagggtt cacttgttag tttgaaaaga
     3901 tttaaagaaa gtgtaagaga agttcctaaa aatactgaat gtggaattat agtttctgat
     3961 tttaccaatt ttaaggaagg agatataatt cagtcatata tagaggaaga agttccaaaa
     4021 aatttaacaa aagaaagaaa atgagcgagt gtattttttg tcaaattata aaagggcaaa
     4081 ttccagcaga aaaggtctat gaagatgatg attgtatagg aataaaggat gtaaatccac
     4141 aggcacctgt tcatatttta gtaataccta aaaaacacat tgataagtta tacaatttag
     4201 aagatgaaaa attacttggt aaaatgttaa ttgtttgttc aaaagttgca gaaatgttta
     4261 gtataaaaga aaatggatac agaattgtta taaatactaa taagtttgct ggacaaagtg
     4321 ttgagcattt acattaccat ttaatgggtg gcagaataat gaattggcca cctggttgat
     4381 aaatagtaaa ttaaaaatgg aaaatgtaaa attcaaaatt ataacttaaa atttaaaaag
     4441 tttttatctt ttgcaatttt caattttaca ctttattttt acgctttaca ttttacatta
     4501 ttaaaacatt gtgctttgct aaaaaacttt tttaatattt ttataatatg acaaaagaaa
     4561 ttagaataaa aaatgtaaaa ataggtggag aaaatatttt ttgtttaata ggtggaccat
     4621 gcattattga aaacgaagaa attgttttcc aaacagcaga gacacttaaa aatatatgtg
     4681 aaaaacatca aatcccgttt atattcaaag catcttttga caaagcaaat agaacatcaa
     4741 taaactcata tagaggacct ggaattgaaa aaggacttga aattttaaat aatgtaaaag
     4801 aaaaacttaa tatcccgata acaacagatg ttcattgtat agagcaaata gaaaaagttg
     4861 caaaagttgt gaacctttta caaattcctg catttctttc aagacagacg gaccttataa
     4921 ttgaggcagg aaaaacaggt aaaccagtaa atatcaaaaa agggcaattt cttgctcctt
     4981 gggatattaa aaatataatt gaaaaatttg aatcaacagg aaacaaaaac cttttaataa
     5041 cagaaagagg tgtctgtttt ggatataaca atcttgtagt tgatttcaca ggtattccaa
     5101 ttatgaggtg ccttggatat cctgttattt ttgatgccac tcatagtgtt caaaaaccag
     5161 gtggaatggg aaaaaaatca ggaggaaata gagagcttgt tccatatctt gcaaaggcag
     5221 ccattgcagt tggagtagat ggtatattta tggaaattca tcctgaacca gaaaaggcac
     5281 tatcggatgg accaaatatg ttaaaattag aggatgttga agaacttttg ataattttaa
     5341 aaaatattta ttatgcaata aaaaaatgag taattatatt ctccgaatga cctggtctgt
     5401 atgacctggc gtaaagaaaa tttactttat aagaaatgta ttgtaatatt ataagaggag
     5461 aagaaaatga gtgaattaaa aatagaggaa agaattaaaa aattgccgtt atatcttcaa
     5521 aaagaagtgg aggattttat cagttttctg ttagaaaagc accaaaaaat atcagggaag
     5581 aaatcactca ggactgggct gatgcattaa aacaataccg ttccaagtac acttcattgg
     5641 aacttcaaaa agaatcatta aagtggagag gggattgatt aatgtacctt gttgatacta
     5701 atgtatggtt agaacgactt cttgaccaga aagaattacc agcagttaaa aattttttaa
     5761 ataaaatatc ttctgaactt ctttttataa tggactttta ttttcactca atttctctaa
     5821 ttatgattaa acttggacaa cgaataggga aattcaattt agattttgat gatacatatc
     5881 aatatgtatc tgctgaaaag tacggtctaa ctcttgtaag ttttgatagt gattttgaca
     5941 gtactaaacc agaggaaatt ataaatatat agcaggtata attttggtat taaggaaaac
     6001 cattgagatt gattaaggaa agtagcaagt gtaaatgaaa ggtacagaga cacaaagtga
     6061 aaaccaaaag ataaaattcc ccctcacctc catcctctcc cctgtgggga gaggcagaaa
     6121 gagagaggga tatttcctgc tcccttggag gagaaagctc gcccgtagag ctacgggcga
     6181 gggtggaaat gaggggaaac ttttctctct gctctacact taaatatgaa caggttttat
     6241 taataattta ctaaaaaaat gagtaaaaaa gaaataatag aatttgggaa aagaattatt
     6301 gaaattgaat gtcagaatct ggaaagagta aaagagaatt taggagatag ttttgctgag
     6361 gcagttgaaa cattgaaatc attagaaggg aaagttgtaa taacaggaat ggggaaaagt
     6421 ggtattattg gaagaaaaat agttgggaca tttgcatcaa tcggaatttc ttccatattt
     6481 cttcacccag gtgatgctgt tcatggtgac cttggagttg tttctaaaaa tgatgtagtt
     6541 cttttaattt caaatagtgg agaaacagat gaactaatta gaattgtccc atcaattaaa
     6601 aaaattggtg caaaaattat atcaataact gctacaacaa aatcaacact tggacaatat
     6661 agcgatatag taattgaaac aggagaaata acagaagcag ataattttgg aataattcca
     6721 tcttcaagta caaccactgc tcttgtaata ggagatgcat tatctttaac tattatgaac
     6781 ataaaaaata ttaaaaagca ggactttgca ttttttcatc cagcagggaa tctcggaaaa
     6841 aggttaatgt taagggttaa agatattatg caaacaggag aaaatattcc aatagttgat
     6901 gagaacagga cacttattga tggtataaaa gaaataaata gaaaaaattt gggctttaca
     6961 cttgcaatta acaaaaataa ctgtttaaca ggtataataa ccgatggaga tataagaaga
     7021 ttacttgcaa aagacataga tattacgaaa ttaaaaatta aagaggtaat gattaaaaac
     7081 ccaaaaacaa ttgatgaaga tattctggct gtaaaagcaa tggaaattat ggaaaagtac
     7141 gaaattacgg cactaccaat agttgatgaa aataaaaaga taaaaggagt ggttcattta
     7201 catgaccttt taggaaagaa ggaatttgga attgagtatt aaaaatataa aaatttttat
     7261 atctgatgtt gatggcgtaa tgacagatgg agggttattt tatataaatg attgccttta
     7321 cagaaaattt aatgtaaagg atggtattgg gataaaaatg ctaaaaattg tgggaataga
     7381 gatagcaatt ttgagcagta atgtttcagt tcaaacaaaa aataggtttc tatcacttgg
     7441 ggtaaatttg tattttgaag gaataaaaga aaaagataaa tttattgaag gctttttaaa
     7501 ggaaaataat ttacagtggg acaatgtatg ttatatgggt gatgacttac aggatataaa
     7561 acctataaga aaagcatctt tttcaatatg tccttctgat gcagttgaag aaattaaaga
     7621 aatagttaat tatgtttgca aaagaaaggg cggagaaggt gcttttagag aaggtgttga
     7681 aaaacttaca aaaaaaatag gaaaatggga aaaaataaaa aaagaatatt tattataaca
     7741 gttttggcat ttttttgtat tttaatttta ccagggtgta atagaaaaca ggtagaaaat
     7801 aaaaagattg atgaaagtca attaaaaaaa ttttctttta aacaattttc tgaaaatttt
     7861 aatttaacaa taattggaga aggagcagaa ataaatgaaa aagatataaa agtaaatagt
     7921 ccatcttttt atataaaagg tgagacagac attatagaaa taaaaacagg aaaagaagga
     7981 actgctgaga taacaataca gccagaaaac aaacaaattc aaaatgtagt ttttactgga
     8041 aatataaaaa ttacgcaaaa agataaagta agcgggaaaa taataatgga agcagaatgc
     8101 ggtaaattaa catataatga tatggctaaa gaaatgatta tggaaatttc acctgtaata
     8161 aaaagagaaa acaatatttt ttctggagaa aaaatttatt actttttaga aaagaacact
     8221 ttacaaataa aaggaaatgt aaatgttaaa attattcctg aaaaatagtt ttattttttg
     8281 gtttcttata agtggaattt tttgttctgc tgaaaatatt actataggaa aatataacaa
     8341 ttgttcatca gatgaagtta aaaaagcacc ttattctgga aacatatata ttaaagaatc
     8401 tcaaaataaa tatataattt tagatattga tgaatatctt tctggtgttc tttcaggaga
     8461 aattggtgag gatttccctt atgaagcact taaagcgcag gcagttgttt caaggacata
     8521 tttacttttc acatcaaaaa aaaataaaca aaatgggctt tcctatgata tagagaactc
     8581 aatttataac caggtctata aagtatgtaa tagtcaaaaa ataagaaatg cagttgatga
     8641 gacaaaggac gagattttaa cttttaatgg agaaattgta gaagtctttt ttcatgcctg
     8701 ttgtggaggc aaaactactt caccctcatc tgtttgggga ggaaattata atggtagtat
     8761 aaatggtata atagatgatt attgtaaagg aacaccatat tattcatggg aaaagaaata
     8821 tagtagcgat tatttatccg atattttcgg attatataat atagataaaa ttgaaattgt
     8881 agaaaaagat gcatctggaa gagtaaaaaa tttgaattta attacaaagg gtggtagagt
     8941 aatacaatta tccggacata aatttagatt acaaataaat tcagcaacga atgtatactt
     9001 tgaaaatcca ttacttcttc cgagtaccat gtttgatgtt gaaaaaaaag ataatgattt
     9061 tatttttaga gggtatggat atggtcatgg agttggacta tgtcagtggg gagcaaaaaa
     9121 gatggcagaa agtggaaaaa attacagaga aatattaaaa ttcttttttt cagatatgga
     9181 aatcatatca ttgggagggg gagaaaatga aaattaatat acattcaaat atagaattag
     9241 atgataaatt tcaagagtac ttaaataaaa aagttgaaaa attagaaaag tttatttttg
     9301 atgaaggtaa tgcagatatt tatataaaaa aagatggtcc attttatatt tctgaaattg
     9361 atataaaaac aaaaaagcat actattttct tgaaagaaaa agagaaaaat atatctaaat
     9421 gtatagactt tctaattgac aaaacaaaaa gaaaattggc acaattacac gatatagtta
     9481 ttgacagaag caaaaaataa ggtcatatta aaagaatcca aaaggaggag aaaaaatgaa
     9541 aataactgat tttcttgata aaagatgtgt tataatcgga atgagaagta gaacaaaaaa
     9601 agaagcaata aaagaactac ttacaaaatt gaaagaaaat ggatttatta aggatgaaaa
     9661 agaaatactt gaaacagtta tggaaagaga aaaattaggc tcaacaggaa taggacaggg
     9721 aattgctgtc cctcatgcta aaagtgacca gattgaaaat cttgttgctg cccttggtat
     9781 ttcaaaaaca ggaattgatt tcaattcctt agatggagaa caagtcaaca ttgtctttct
     9841 cgttcttgct cccactaaat ctgttggact gcatcttaaa gcacttgcaa agattgcaag
     9901 attgttaaaa gacagggtct tcagaaatgc tttgaaagat gctcaatccc cagaagaaat
     9961 aattgattta ataaaagaag atgaacaaaa acttaataat gtaggataaa attttcttcc
    10021 tgttataaaa atttttataa ggacttgaaa tatatggaaa aattaaaagg aattggtgtc
    10081 ttttccgata tagtatttgg gaaaggcttt gtctggaaaa atttttttct tttgtctcag
    10141 gaatacaaaa ttcccaaaaa agatattaaa gaagaaataa ataaattgga tgaagcaatt
    10201 aaaaaaacaa ttgaagaaat tactgcatta aaaaaaaact tagaagaaag tataggaaaa
    10261 gaatatgcag atatattcaa ctttcatctt tctattcttc aagataaaaa tttaagagat
    10321 gaaactgtaa aaataataaa agaagaacaa cttaatgttg aaagttctct aaaaaaagta
    10381 atttttaaat taggtgaaat atttggaaaa agtgaaaagg actttcttaa agatagaaga
    10441 agggatattc ttgatgttgt tgaaaaaatt gtttcaaatt taaaagatat accctctatg
    10501 aaagttattg gcgaaggaga aataattgtt gcagatgact tatcaccatc tcaaattgta
    10561 gctctcaaca gaaaacttgt aaaaggtatt gttactgaca taggaagtga aacatcccat
    10621 attgcaatta tggtaagggc acttgaaatt ccatctgtta ttggtgtagg agaagcaact
    10681 aaagtaataa aaacaggtga tggactaatt gtggatgggg aagaaggaat agttatagtg
    10741 aacccaacca accaggtaat aaatgagtat aaaaagaaaa aagaggaatt gaagaagaaa
    10801 aggaaaaaaa tatcactttt aaaaaattta aaatgtaaaa caaaagatag aaaagaaata
    10861 aaattattcg ctaatattgc atttcctgaa gaaatcattg tagcagaaaa aaatggatat
    10921 gatggaatcg gtctttatag aacagaatat ctttatatca atagaaaaaa tttaccggat
    10981 gaagaagagc aattttatgc ctataaaaat attgcagaaa gagttaaaaa taaaccagta
    11041 ataataagaa caatagatat aggaggagat aaaattcttc caggtatctt tgaaaaaaaa
    11101 gaaacaaacc catttttagg gtggagagga ataagatttt gtttggacag aagagatata
    11161 tttgaaacac aaataaaagc aattttgcgt gcttcggttt atggagaaat taaaattatg
    11221 tttccaatgg ttgcaacatt agaagaagta attgagggga aaaaagtaat agaagatgtg
    11281 aaaaagaaat taaaaagaga aaataaaaaa tttaaagaaa tagaattagg aacaatgatt
    11341 gaaataccat ctgctgcttt aatttctgat aaacttgcaa aagaagtgga ttttttctct
    11401 attggttcta atgaccttat tcaatttact cttgcagttg ataggttaaa tgaaaaaatt
    11461 actcatcttt atcagccctg ccacccatct gttttaaaaa tggttaaatt aacaattgaa
    11521 aatgcagaga aaaacaaaat tcaagtagga atatgcggtg aaatggcttc catacctgat
    11581 atcgcctgtc tactcgttgg tatgggaatt gatgaattaa gtatggctcc tatttcaata
    11641 ccactggtaa aagaaaaact tataaataaa aaatatcagg acttaaagga aatatcagaa
    11701 gaggttttta aatttaatac aaataatgaa attataaatt tcttaaaaga ggaattaaaa
    11761 taagggggaa aacaaaaaat gattgaaaaa gagataaaga aaattgataa atcaaatatg
    11821 cgtgatttac ttataaaatt tggagagcat tgtataagtg gatattctct tcttccctca
    11881 tttattcctg aaaacataaa atttaacaaa attatttatt gcgggatggg cggttctgct
    11941 ataagtggtg atatattaaa atttattgtt gaaaaacatt ctcatattcc ttttgcaata
    12001 agtagagatt acgatatacc ttcttttgct gataatgaaa caattgtatt tataacaagt
    12061 tattctggaa atacagaaga aactatttcc acttttacac aggcaattga aaaaaaagca
    12121 aatactttta ttatttcaag caatggggaa ttagaaaaat tatcagtgga aaaaaattta
    12181 ccatttataa aaattccttc tgggatgcct ccaagatgtg cttttggata tttgtttttt
    12241 gcatcttaca gaatattaca acaatttggt ctccttccag aaattgagaa aaaacttttt
    12301 caaaaaattg atgaaattgt caaatcattt tcagagacag agaataatta tgctattacc
    12361 atagcaaaaa aaatccataa taaagttcct attctctatt cggataattt tatatttgga
    12421 tgccttttga gatggaaaac acaaattgct gagaattcaa aagcattttc atttataaat
    12481 gttttccctg aaatgaatca taatgagatt atgtcttttc attttccagt gtggttctta
    12541 aagaaaatta tatgtttatt tttcatacat aatgaagaaa ataatagaac taaaaaaaga
    12601 tttgaaataa cagaaaaaat aatacaggga aaaggaattg agacattaaa agttgaagga
    12661 aaaggtaatt cacttataga aaaaatgctt tattttgtaa ttcttggtga ttgggtaagt
    12721 tattatcttt ctcttttaaa taaaatagac ccaactgaaa taaaagaaat aatatattta
    12781 aaagaccaat taaaaggagg aaagtttgag taaaacattt ttatttacat ctgaatcagt
    12841 aaccgaagga catccggata aaatatgtga ccaggtatct gatacaatac ttgatgaggt
    12901 attaagtcag gataaaaatg gaagggtcgc atgtgaaaca cttacaacaa gaggattaat
    12961 ttttgttgca ggagaaattt caacaagggg ttatgttgaa attccatttc tggtaaaaaa
    13021 tattcttaaa gatattggtt atatagaacc atcttttggt ttttactatg attcaattgg
    13081 aattataact tccattcaag aacaatctcc caatatagca attggtgtta ataaaggagg
    13141 agcaggagac cagggaatga tgtttggata tgcaacaaat gaaactcctg aattgatgcc
    13201 tcttccaata attcttgctc ataaacttac aaaacggctt gcagaagtga gaaagaagaa
    13261 gattgttgat tatttaagac ctgatggaaa atcacaggta actattaaat atgaaaatga
    13321 aataccaaaa acagttgaat gtgttgtttt atctgctcaa cacaatcctg aaatttctca
    13381 taaaaagata gtagatgact taactgaaac tgttataaat gatataatac ctaatgaaat
    13441 tcgcagtaag aaaacgaaaa tttttataaa tccaacaggt attttttcaa taggtggacc
    13501 tattgctgat actggtgtaa ctggaagaaa aattatagtt gatacttatg gtggagttgg
    13561 cagtcatgga ggaggatgtt tctcagggaa agacccgaca aaagttgata ggtctgcttc
    13621 ttattgtgcg agataccttg caaaaaatat agtcgcaagt ggtattgcca aaagatgtga
    13681 aatacaactt gcctatgtaa ttggtttaaa acaacctgtc tcaataatgg taaatacata
    13741 caatacagga attatcccag aagaaaaaat atcaaaaatt attagagaaa atttcgacct
    13801 atctcctgct ggtattatca aaaaatttga cttattaaga ccaatttata gaaaaacagc
    13861 gtgttatgga cactttggaa gagaagagaa agaatttaaa tgggaatata tagatagtgt
    13921 tgatatgttt aaaaaaagtg cttagacaac ttatgttgac tgaaagtagc ggtagtaaga
    13981 ataaatttaa aattgaaagt gtaaaagtta aaattacaac ataaaattta aaactttcgt
    14041 ttttgtattc tatccgataa aaagttcacc atacaacatg gcaaatctga catagttcct
    14101 ggcgcacatt acggacctgt ccgctttctt tttggagaat attgaaacac tttgtgacta
    14161 tgtgcttaaa taagcattgc ttatgaatat ttatatttaa aaaggagacc tgatggaata
    14221 ttcaataaaa gatattggtc tttcagaaac tggggaaaaa agagttaatt gggcttacaa
    14281 tgaaatgaaa gttatttccc ttttaagaaa ggaattttta ggaaaaaatg cttttgctgg
    14341 aacaaaaatt gggtgttgtc ttcatataac atcagagaca gcaaatcttg ttataaattt
    14401 caaaaaagca ggaggtgatg tatatctatg tgcatcaaat ccattaagta ctcaggatga
    14461 tgttgcagca tatttagtaa aagaagagaa aatacctgtc tttgggaaaa agggagaaaa
    14521 tgaaaaagaa tattatgaaa acataaaaaa agttatggaa aaaaatctca atcttgttgt
    14581 tgatgatgga ggagacctta ttactgccct tcacagaagt cctgactatg ctaaaaatat
    14641 aattggagca actgaagaga caacaacagg agttatacga ctaaaaaata tggcaaaaaa
    14701 gggaatatta aaattccctg ttattgcagt aaatgatgca aaaacaaagc atttttttga
    14761 taacagatat ggaaccgggc aatcaacaat tgatggtatt ttaagaagta caaatatact
    14821 tcttgcaggg aaaattgctg ttgtttgtgg atatggatgg tgcggaagag gtattgcaaa
    14881 aaggtttgct ggaatgggcg caaaagttat aatatgtgag gtgaacccat taaccgcatt
    14941 agaagcaaca atggatggtt tttttgttat gccaatatca aaagcagcag aaataggaga
    15001 tatttttata acagcaacag gaaatagtag tgtaattaca ataaaagaaa taatgaaaat
    15061 gaaggatgga gcaattcttg gaaattctgg acatttcaat gtagaaatag atgtaaaaga
    15121 acttgaaaaa atagggaaaa aaataagaat aagagaacaa ttagaccagt acactctccc
    15181 gaatggaaaa aaaatttatt tattaggaga aggacggctc ttaaatcttg catgtgcaga
    15241 aggacatcct tcatcagtta tggatatgag ttttgccaat caattccttt ctcacctgta
    15301 cctaaaaacg catcctaact taaaaccaga tgtttatgat gtccctgagg aaattgataa
    15361 aaaaattgct gaattgaaat taaaatcact taatataaaa attgataaat taaccagaga
    15421 acaaaaagaa tatcttaatt catggaaaac aggaacataa agaattaatt tttatggaaa
    15481 aaataaaaag agttggagta acaggtatat ttggctctgg aaaatcaaca gtaacttcaa
    15541 tttttaaaaa atatgggata gaagttattt cctgtgatga gatagtacat tttcttcttg
    15601 aaaaagaaaa tataaaaaag aaactcatca aaattttcgg aaaagaaata gttaaagatg
    15661 ggaaaattga tagaaaaaaa ataagtgata taatttttaa aaataaactc aaaaaaaaag
    15721 aacttgaaaa tttaattcac ccactcgtct ttaaggaaat agataaaaaa ataaaaagag
    15781 atattgacaa aataaaaaag aaaggtataa taatcgtaga aattcctctt ttatttgaaa
    15841 caaaatcaga aaaaaagttt gattttattt tagttgtgag tgcatctcct gaaataatca
    15901 aagaaagact aaaagaaaaa tttagtagta aagaagtcga attaagatgg agaaatcaga
    15961 ttcctctaat atataaagaa aaaaatgcaa actttgttat tgataattct ggaacaatta
    16021 gtcaaaccga aaaaattgtt aaagaaataa ttaaaaaaat catatcacaa taaaattata
    16081 aatgaaaatt tctcttattt ttcttcaatt atccatgaaa aataaaaagt taagaaaaaa
    16141 actaattaaa atttggcaat attccaacaa atatggggag gaaaaatgga tcaaaaagaa
    16201 ataaatgttt caaatcttat tacattgaca atcccaaaac ttcaaaagat agcaaaagaa
    16261 atgggaatta atggaataag tggactgaaa aaagatgaat tggtgtacaa aataatggaa
    16321 acacaaataa acaaaaatgg agttttattt ggagagggag ttcttgagat tttaccagat
    16381 ggttttggtt ttttaaggat gccaagttac aattatcttc caggtcctga tgatatttat
    16441 gtctccccat ctcaaataaa aaaatttgga cttaaaacag gtgataatgt ttcaggacaa
    16501 ataagaccac caaaagatag cgaaaaatat tttgcacttt taaaagttga aatggtaaat
    16561 ggtcaaccga ctgaaaaact acaaagtaga attccctttg aagaactaac tcctatacat
    16621 cccaataaaa ggtttatact tgaaacaact tctgatgaaa ttacaacaag agtaattgat
    16681 ttagttactc cgattggtaa aggacaaaga ggtcttattg tttctccacc tcgtgctgga
    16741 aaaacagtaa ttctgcaaaa aatagcaaat gcaatcgctg aaaataaccc agaagtagtt
    16801 cttattgttt tgcttgttgc agaaaggcca gaagaagtta cagaaatgag acgtcttgta
    16861 aagggagaag ttataagtgc cacttttgat gaagcacctg aaaggcatat acaggtatcg
    16921 gaaattgcct tagaaaaagc gaaacgactt gttgaacata aaaatgatgt tgtaatactt
    16981 cttgacagta ttacacgact tgcaagagca tataatgttt tagttccaca tagtggaaaa
    17041 atcatgacag gtggtcttga atcaactgca cttgataaac caaaacgatt ttttggtgct
    17101 gcaagaaaca tagaagaagg cggaagctta acaataattg gaactgccct gattgacaca
    17161 ggaagcaaaa tggatgatgt tatttttgag gaatttaagg gcacagggaa tatggaacta
    17221 aatttagaaa gaaagttagc cgatagaaga actttccctg ctattgacat taataggtca
    17281 ggaacaagaa gagaagaact attagttgac ccagatgaac ttcaacttat gtggttattg
    17341 agaaaaatgc ttgcaccatt aaatgaagtt gaagcaatgg agaaaatgat aactatgata
    17401 aaaaacactc gctcaaatat agaacttctt ttaagtttaa aatcaaaagt taaagaattt
    17461 gtaaaataac ctttttcttg tacatatcac aatttgtgga aaattattca gttagattta
    17521 ttatgattta attccttcta ttgacaaaaa aagatttttg atgtatcttt aatgtagata
    17581 cttaatagga ggcattgata tggaaactcg tattcaaaaa tggggcaata gtttcgctat
    17641 ccgtattccc aagacaattg ccaaagaaat tggaatagag aataattcac ctgttgagat
    17701 actactaaaa aatggaaaag ttgttatatc tccaaaagtt aaacccaaaa tgaatttgca
    17761 acaacttcta tcaaaagtaa ataaaaaaaa tctccataat gaagtggata ctggttctgc
    17821 aatgggtggt gaagtgtggt aaaaaagaaa aagtatatcc cacagcgtgg cgatgttatt
    17881 tatatttcct taaatcctca ggcaggacat gaacaagcag gacgcagacc tgctgtagtg
    17941 atttcaccat tagcatataa taacaaagtt gggctggtta ttctctgtcc tattacaaat
    18001 caaattaagg gttatccatt tgaagtaatt attcctgatg gactaataat aacaggtgca
    18061 atactttctg accagataaa aagtttagat tggcaggcaa gaaaagcaaa atttatatgc
    18121 actttaccag tagagacaat tcatgaagta ttagaaaaat tatctgtttt attgttagag
    18181 tgatggtaaa aaaagatatt attttaaaga gtcctgtgag aggaaatgga aaatgacaat
    18241 tatttataag aatagaattg aaggattaag ttgtggatgt acatcggaag acatagcaac
    18301 ttttaaagtt attgaagatg ggattgaatg tatctggtgc ggaaacaaaa taaattgcaa
    18361 tttgcctgtt gaatttgtta tgaaagaata ttgtgagaac tgtggaaaaa aaacaacttt
    18421 tattttagat acaatacaaa tgaatttctg cacaaaatgt ggtaaatcta aacaatcaaa
    18481 ggataaactt aaaaaataaa aaaaacgaaa atcccatcaa aaacacgcca caaatattac
    18541 ataaaattac atatatattt tctctaataa attttttatt ttttcataaa gcaaatgaaa
    18601 caatagaata ccagagaaaa tcagcaagaa tatgcccaac aaaaaataaa aaaacacctt
    18661 ttactccaaa tttcaaagaa atactaaaat atatggttct ttcttaaaaa agttgcagta
    18721 atattttaaa gttattgata ttcaaagaga tatggaaata ttaaagttag gagagaaaat
    18781 ggatttaaac acactaacaa aaatactaaa tattgaagga tgtaaagtag tagaaatttt
    18841 atctttaaca gaggaagaaa tacatctaaa aatagagcca tataagagga aaccagcaat
    18901 atgttcagga tgtggagagg aacatataca aggatatcat agtgaaaaag aagtaatagt
    18961 agaagattta cctataagtg aaaggagagt atttcttcat gtaaagaagc ggttatatcg
    19021 ttgttcaagg gataacaaaa tatatacaga gaaaatagaa tggttaaata agaggtcaag
    19081 atttacatat agatttgcca agcaagtaaa cagattaacg gctataacga caaatcaaga
    19141 ggcggggtgg tatttaggat taggcgatga agtagtttat aggatataca aagagatgtt
    19201 ggaaaaacag gcaaaagaga aattaatacc aataccgagt gcaattcata tcagcgtaga
    19261 tgaagtaatt tataagaaca caggtattta accaatgtaa ttgatgtaga taaaagagat
    19321 gaattaaaca aagcgaggaa agaagggaat gaagaattag tagaattaac aaattgtaag
    19381 cagcgattta ttctttttaa gaataaaaat aaacttaatg aaaaacagaa gggatatctc
    19441 gataagttat atgaaataaa catttctata tataaataga tgttattaaa agagagtttt
    19501 ttagaagttt atacacatga aagagttgaa gaagcaaaag aatgcttaga gaattggatt
    19561 aagcaggcat tatcaagtgg tttagatgta tttattcaat taggatataa atgtcaagag
    19621 aaaatgaaat atattctaaa ctggttttat aagtccacag gatcctttgg aaaagtaagt
    19681 ttcgctattt cagaagggtt taataataaa attaaaagat taaaaagtcc gcaggatccc
    19741 tcgggcgtag tcctgagggg atccgtagcc aaaagcgagg accctacggg cgagcctgtg
    19801 gaaatggcat atggatataa agatataaat tatttcaggt taaaaattca tcaatcatgc
    19861 ccgaggcaga tccgcttctg gcggacattg cggtttatta aaccctagat taaatacttt
    19921 aagtgcaact taaataagaa agaccctatt tagatgctaa aaattagggc tgctttaatg
    19981 gggttcagtt aggatttaga tttttatatt tatcttatta cataaaagtt tgtcaaaatt
    20041 atttttatcc tctatctccc cttcaatttt caaaaaataa aaagggatat taccttttat
    20101 aaatttttga actttcacat agtccttttc agtagttaca ataaagtcaa tattttcttt
    20161 tttaaaaaat tccattattt catttatatc tttttctttg taattaaaat ggtcaggaaa
    20221 aacaacagaa tattgttttt caggttttaa ttgggaaatt gtcagaaaaa aattaaaagg
    20281 gtttccaatt ccagcaaaac aaagaatttt tttacctttt acttcttcta aaagaacttt
    20341 ttctttacca tttgaaagat aaactggttt tatttttaaa aaaaatattg gtttttcctg
    20401 tttttgtaaa aaagtaagaa gagtatattt ttccttctct ccaactaaat gaggataact
    20461 tattataaaa acatctgctc tttttataga agaaactggc tctcttaaaa gtcctgatgg
    20521 aattaacaat ttgttatcaa aagggtttga agaatcaaac ataagtatat caacatcttt
    20581 ttcaacccac tgacaatgaa aaccatcatc aattacaact atttcaattc ccatttcttc
    20641 aagtttttcc aaaccagatg gtgttttatc tcctgaaata attattgaag ggaaattatt
    20701 tttaatcatt atcacttcat catatggaat actctctcct ttttcttttt taaaaataga
    20761 actgacaact gcaattttat aattcaagga aatgactttt ttaataattt cacttaccaa
    20821 taatgttttc cctgttcccc ctgttataat attcccaaca cttataataa aaaatttata
    20881 ctttcttttc tttgagaaac acctttttaa tgttattaca aataaatata aaaatgatat
    20941 aggcaaaaaa aacaaagaga ccaatttaaa aaaaacagac ctttttctaa ataaaaattt
    21001 cctataaaaa aagttaaaaa agggtcggct ggtcttcaat tgaacaagat tcacatggta
    21061 tttcaacttt tccgaattca ccgtcatatc ctggatttat tttaacattt ccttctctta
    21121 cacttataat accttttgca attttttcat caattgtatt taataattca ttttctggta
    21181 tttgaataag aacagagaat tctgaacctg tttttttaat aatttcaata taatgatttc
    21241 taacttctat tgagtcctca ccttttttat aaacattact tataatttga ataagaggta
    21301 caagtttttt aaaatcaatg tatttttttg gtctttctcc tctttttcta tcagcaaggt
    21361 cataaactct gtgcataact ccaatcgtca agtttctacc acaaacagga cacttattgt
    21421 tattaactgc actttcttct ggtgaaaaac aaacattaca atttctatgt ccatcaaaat
    21481 gatattttcc ttcttctgga aaatactcaa ttgtcattaa aaatttatct ttatccttat
    21541 ttcttaaaat ttctattaac tcaaaataat taaatttatc tttaaaaaca ttagattctc
    21601 tgccaatttt taaaggagaa tgagcatctg aattagaaat taaagagaac ctatcaagag
    21661 gtgaaaccat ccagttcatt tcagggtcac tacttaatcc tgtttcaaga gcaaaaattt
    21721 cagaagtaaa tctcccaaaa caatcgtaaa tagaattaaa accagaattt gcaccaaaca
    21781 cggagaaatg cggagtccat atatgtgcag gaacaacaaa tatatcttca tttatttctt
    21841 ttataatgtt caaaaggtca atacaggaaa gatgtaatat tggtcttcca tcagcgttaa
    21901 ggtccccata tttttctatc ttcctgttaa atttttctgc tacttcaaaa gaaggaagaa
    21961 aaattatatt gtgaattttt ttaataacat cgtcttgctc aaaaatattg ttcacttccc
    22021 ctgttaaaat aaaatcaact ccattaaaag tatatatacc atatctatct gtttgtttga
    22081 gttttccttt taactcataa aaccacataa aatgtgtaat atctcctgtc ccgagaatat
    22141 ttattccctt gtattttgcc cattttgtta attcttcaat taccatattt ttacttgttg
    22201 ccttactata ttttgaatga atatgaaaat cagcaatata cattttttcc ctcttattat
    22261 ttgaattctt ttataaggac agaataaagt tcagaaagtg attgtggtat aacttttgta
    22321 ttggaaatca caggcataaa attagtatca ccattccatc ttggaactat atgaaaatga
    22381 atatggtcat caagccctgc cccggcaact tttcctaaat tcagtcctat attaaaacca
    22441 tctggattta atactttttt taaaatacta actgatttct taatcaactg cattatttct
    22501 atttcttcct tttggttaac agatgtaata tcttttatat gccttttagg tgctaccatt
    22561 agatgtccat tattatatgg gaaaatattc ataataataa aagaaacatc tcctctataa
    22621 acaacaaaat tttctttgtc tttttttgat ttataaccat agcataaaaa acattttctt
    22681 ttttcaattt tatttatata cccaattctc catggagccc agattatttc tttattcata
    22741 gttcttcctc tgttattact ccaattctat tgtctaacaa aaactgtgct gtaacaccat
    22801 ttccttcaat tattttttta ctaaaactac catcataaat tttccctttt ccacatgcag
    22861 gacttaattg ttttaaaatt acaaagttca cattatacat ttttaaaatt ctaaaagtaa
    22921 tttctgcccc ttttacataa tattctgtaa catcctcacc ttttttatta ataactctac
    22981 actttttatt caaaacatct tttccatttc cgtattttat ttctgctggt tctctcaaaa
    23041 caggcaatcc accaagaatt tctggacaaa aaataattaa atttttctta ctgataaaat
    23101 taacaacttt cttattaaga gaatttcttc catcatatct acaattctca ccaacaagac
    23161 aggcacttac cccaacaaat tcctctatca tttcaattgt agttcttttt ttattttatg
    23221 taaaatctct tttttatttt ccttcaaata tcttttaaga taagtttttt tgttaggcaa
    23281 tattccatct aagaccaaat tttcaaggta atttttaaca aatccaactt ccttaccttg
    23341 tttaattgtt aaaatatcca ttataacaaa accgtcaata gcaagtttaa acgaagataa
    23401 cttatctttt ttatttattt cttttattct ttcttcaagt ttctctaact tttcaattgc
    23461 tttttttacc tttatttcat ttttacttgt aatatctgct tttgcaagtg taaaaagtgc
    23521 ttttatatct tttcccactt cttttataaa tcttcttaaa gcactatcag ttggattctc
    23581 ttttgctaaa agatgtggcc ttaaatgaaa tcttataagt tttccaataa attttgcatt
    23641 ttcatcgctt attttcatcc ttttacatat tttataactc attttttcac ctaaaaattc
    23701 atgtctatga aaactcactt tcccattttc aacttttaag gttgctggtt tacctatatc
    23761 atgtataagt gcagaaaatc ttaaataaac atttcttgta tgtctgctaa tattatcaag
    23821 tgttttcaat gtatgctcaa ataggtcctt acatggatgg tctgttcttt tttctttaag
    23881 agaaaaaact tccggtaaaa caatttcaag aactccaatt tcatccatca aataaaatcc
    23941 atccgatggt ttggttgctg aaataatttt taaaatctca tcagttattc tttcctggct
    24001 aactattttt attctataaa catttttttt catactttct tttgttttat cttctatctt
    24061 aaattttaat cttgtagaaa atcttatgcc tcttaaaatc cttagcgggt catcataaaa
    24121 tgttttatcc gggtcggttg gtgtttttat aatttttctc ttaatatcct gtcttccctc
    24181 aaatgggtca agtatttcac ctgtaattaa gttttctgca attgcattga tagtaaagtc
    24241 ccttcttaat aaatcctgat atatattaca tttctcaaca attggttttc tggtatcatc
    24301 tggataactt tcttttctcg cagttgcaaa ttcaactttt attccattga tttcaatcat
    24361 tgctgttcca aaccttccat aaaatacagg tgtaggcaaa ttatacattt ttgaaaattc
    24421 atttgctatc tttaaagcat cctcttccat aacaaaatct acatcaacac ttttctttct
    24481 taaaagattg tctcttatat agccaccaac aagataaata tctataccct ttttctttgc
    24541 aaaatccctc atcttttcaa ttattttttc tatttgaatt tttgtttttc ccattttagt
    24601 tcttacccat tttaaaattt tcttatattt taccatcctt attgtttttt taaactataa
    24661 ttgatataaa tttcaaatag aagcaataat acatttataa aattttctaa tttcttttga
    24721 gtgaagaagt taagatacat aggtaacacc tgccagtttt ttgaaaagtt tagggattta
    24781 tataaaatag aggaagaact gggaagaaaa aatgggatac aagtatgcaa agaagttcat
    24841 accattaagg aaaggagaag tagtaagcaa agggtgtgag atagtccatt tatcgaagaa
    24901 aggtaaagag agattaaagt ggataaagta ttataagagt catgaagaga atgcttcatt
    24961 ggtaagtagg tattatggga taagtaggaa aacattttat aagtggtatg ggaggtataa
    25021 actattagga ttaaagggat tagaagatat gagtaaagca cctataaata aaaggggaca
    25081 tgaaattacg tgggaagagg aattaaggat aaagaagtta aggaaacaat atataaggta
    25141 tggaaaagag aaactatcag tgctttataa gaagatatat ggagaaaaga tatcggggtg
    25201 gaagatatat caggttataa ggaaatacaa tttatattgg agtccagtaa agaatgagaa
    25261 attaaggaaa aagagaaagt tggcagagaa gaaaaagagg ataacagaat taaagaacaa
    25321 acagctggca gggtttttct ttca
//
LOCUS       DAOOVW010000026        25293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_1324_length_25293_cov_8.335367, whole genome shotgun sequence.
ACCESSION   DAOOVW010000026 DAOOVW010000000
VERSION     DAOOVW010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 25293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25293
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1324_length_25293_cov_8.335367"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="PLW95_04860"
     CDS             complement(<1..740)
                     /locus_tag="PLW95_04860"
                     /inference="COORDINATES: protein motif:HMM:NF018218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HOV21995.1"
                     /translation="MDENKQKNKNKKQQIKVPTNNIFKGLIFWIIIGIILFSLLRFSE
                     YEGAKKNLIYSDFIKQVESQNINGTLKIKNGYIYGELKDGTKFYTYCGEDNELYKILR
                     ENNVSFAPEPSSSFWSNLFISVIPIIFLFLLMWFILFKQVSTEGNRVMSFAKSRPIMP
                     DNKNRITFDDVAGCEEAKEDLKEIIEFLKNPKKFVRLGAKIPRGVLLIGAPGTGKTLL
                     AKAVAGEAKVPFLSMSGSDFVEMFVGVGA"
     gene            complement(695..1753)
                     /gene="thiE"
                     /locus_tag="PLW95_04865"
     CDS             complement(695..1753)
                     /gene="thiE"
                     /locus_tag="PLW95_04865"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOV21996.1"
                     /translation="MDKRVRIIDANFNRTREGLRVIEDGIRFYLENEEIFKKIKDFRH
                     KFIKEVIKNFPIIFLHKTRESSYDVGKNYDVKEKENIKRIIEKNCLRVEEGLRAIEEY
                     SKCIDGKISSIFHDLRFEFYNLEKQITTLISRKKILLPFVYAIINLEDNKNIFSITEE
                     IIKGKVDIIQLRYKGENIKYFLDIAKKLKKVIPDEIIYIINDRVDICEIVDADGVHLG
                     DRDISGEDARKVIPDKIIGVSTNSLEEVLKYKGKDIDYLAIGAIYPSPTKKERKVVGT
                     TIIKDIKKKINIPLVAIGGINKDNVKEVIKNGADGVALISAIEKAKNKEEYLKQIKKK
                     VKKEWMKINKKTKIKNNK"
     gene            complement(1737..2216)
                     /gene="rfaE2"
                     /locus_tag="PLW95_04870"
     CDS             complement(1737..2216)
                     /gene="rfaE2"
                     /locus_tag="PLW95_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015791090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HOV21997.1"
                     /translation="MRKKKSFKEIVKIVNELKREKKKIVFTNGCFDIIHSGHIKLLKK
                     AKQLGDVVIVGINKDSSIKKIKGKNRPILNENQRIEIISAIEFVDYVVPFGQTTPEKL
                     IKIIKPDILIKGGDYKKEDVVGKDIVEKYGGKVYIFPLIGNISTTNIIKKIKNGQKS"
     gene            complement(2216..2788)
                     /locus_tag="PLW95_04875"
     CDS             complement(2216..2788)
                     /locus_tag="PLW95_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOV21998.1"
                     /translation="MNQNNNIEKIKKEIEESIKLKKELLEKENIDRILSAALMIKKCL
                     ENKKKVLLCGNGGSAADCQHFAGEMVGRFKKERKPYPFISLTTDTSILTSISNDYSFK
                     KIFERQVECLGEKGDILICFSTSGESENILLAAHKGKKVGMEIISFTGKEPNSLSKIS
                     SLTLSVPSEDTPRIQEVHCLIYHIICSLVE"
     gene            complement(2772..3338)
                     /gene="amaP"
                     /locus_tag="PLW95_04880"
     CDS             complement(2772..3338)
                     /gene="amaP"
                     /locus_tag="PLW95_04880"
                     /inference="COORDINATES: protein motif:HMM:NF033218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HOV21999.1"
                     /translation="MKIFGRIVSAIYILAGIIFSIFLLIIWANPYYVDILSTFLKKNV
                     AAIGAVGMVLVILGVIYIVNWFEYINKTKNISFDNPGGEVKISLKAIEDSITALISKQ
                     IDGIKSLRVKNYVSPKGLETKFYLKLTSGHNIPETCATIQEITKNYLQDAVGVERVSN
                     IEVYVTNISKEEFLNEKIPEQTEDEPEQ"
     gene            complement(3404..4474)
                     /gene="dprA"
                     /locus_tag="PLW95_04885"
     CDS             complement(3404..4474)
                     /gene="dprA"
                     /locus_tag="PLW95_04885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOV22000.1"
                     /translation="MEDKKYWIGASILKIKPSKLYKFIEKTENIENLFNLPVDELVKK
                     GIDQKSAEKIKNIKTINWEKEVEYAIKNDIKIITIKDKEYPPLLKQIYDPPYILYVKG
                     EIENHPFPIAMVGTRNPSYYGIKMAEKFSFQLSSLGITIVSGLARGIDYICHYGAIKS
                     KGKTVGVLGSGFDNFYPKENIKLVDKIVELGGAIITEFPSSVLPEKWNFPIRNRIIAG
                     ISKGVIVVEAGPRSGAIITANIAISEGRDVFALPGQADSITSKGTNKLLKDGAILIQD
                     ISDILEELNIEIKEQEKKEKIVKNIDEEEKKVLSFINEKVNIEQLLKLTGMDYPCLST
                     ILLKLELKGIIKELPGKNYELL"
     gene            complement(4476..5630)
                     /locus_tag="PLW95_04890"
     CDS             complement(4476..5630)
                     /locus_tag="PLW95_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HOV22001.1"
                     /translation="MKNNQDIKDNDILLSPYAARSSSSLGRKYKEEEHPYRNVFQRDR
                     DRIIHSTAFRRLEYKTQVFIYGEGDHYRNRLTHTLEVQQIARTIARTLKLNEDLVEGI
                     SLAHDIGHTPFGHKGELVLDKLMKEHKFPGFEHNEQGLKVVDKIEERYPDFPGLNLTY
                     EVREGIIRHKTSYDRPLIDEYQEFTKFTSPSLEAQVVNVSDEIAYTCHDLDDGIRSGI
                     ISFKEIEKITLWKEIEKKVNIEKIPEKHKRHVMIRNFINYLVSDVICQSIENIEKSGV
                     SSIEDVRNTHILITHSEEVIRKHTQLRKFLEENMYENYRVIRMAKKAERIITDLFNAY
                     LNEPKQLPPHIQKKMEYEPKEIVICDYIAGMTDRFAQLEYQKLFNLESLV"
     gene            complement(5627..6403)
                     /locus_tag="PLW95_04895"
     CDS             complement(5627..6403)
                     /locus_tag="PLW95_04895"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOV22002.1"
                     /translation="MINFQKLVKKFYKEIEIIGIIFLISGCTFTVGTSTSKKKSEPNY
                     TYHYVKSGENLFRISEYYYDGKSTSEIKVGVEKIKKANNLFSDNISCGQKLKIPGTNK
                     KQPSYALLPPSSNIKQPSSDRNEVETKNEYIPIIKDSAFIWPVEKGDIICKYGELGNQ
                     GIDIIVEQGSTVFSSAAGTVSYVGTTAKYQETIIIEHTNNLYTIYGHDLEILTKKGEK
                     VKKGQVIGKIKGGTQKKRYLHFEIRIGDKAVDPLIYLPEK"
     gene            complement(6458..7096)
                     /gene="nth"
                     /locus_tag="PLW95_04900"
     CDS             complement(6458..7096)
                     /gene="nth"
                     /locus_tag="PLW95_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOV22003.1"
                     /translation="MEKENVNKIIRILEKEYPESKTSLVFQNPFQLLIATILSAQTTD
                     KLVNKITPILFEKFKSPYDFASSPVEEIEKIISSVNFYKNKAKNIKNLCEILVKQYNG
                     KVPDKVEELIKLPGVARKTANVVVSQAFGKSEGIVVDTHVKRVSQRLGLTKNTEPSKI
                     EQDLMKIIPKKNWIDFPFRLILHGRNVCKAKNPECLKCVIKNFCNFFKENNK"
     gene            complement(7811..8125)
                     /locus_tag="PLW95_04905"
     CDS             complement(7811..8125)
                     /locus_tag="PLW95_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22004.1"
                     /translation="MDKKVIDEGKGLAWLSYLGLLLLIPLLVNKDNEYSKFHVKQGLV
                     LLICGIITRILWIIPVIGWVVGMVVGIFLLVLMIIGIVNALSEKTEPLPVIGKYAEKI
                     KI"
     gene            complement(8191..9045)
                     /locus_tag="PLW95_04910"
     CDS             complement(8191..9045)
                     /locus_tag="PLW95_04910"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HOV22005.1"
                     /translation="MKVIPAIDIEENKVARLYKGNYNRVTFYKDTPAFFTKKFIEFGA
                     KRIHLIMLSGAKKGHHSKGNQKIIEEVIETKNRLNPKCEIQLGGGIRKPGQVRHYLST
                     GIDFVIMGTSLLVPILLENGYTIQDLRFLYQQAGKDFKVEKESPEIEIIEKLNRDIKK
                     RIIVAIDFINNEIGLSGWNVTVPILPEFLIQKFIEKGFSRFLITNIERDGTLEGIDIV
                     PIGRVLIKIDNIREKIEEIIISGGVKDENDIIALNSIGYKIDGVVIGKAIYDRTIDIK
                     KVISTFQD"
     gene            complement(8990..10885)
                     /gene="topA"
                     /locus_tag="PLW95_04915"
     CDS             complement(8990..10885)
                     /gene="topA"
                     /locus_tag="PLW95_04915"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOV22006.1"
                     /translation="MKKGVIIVESPTKAKTISGILGKEYTITATMGHVRDLPKNKFGV
                     NIEKNFEPTYVISPGKRKIVSKIKKLTEDIEEIFMATDEDREGEAIAWHTTIAIGKEI
                     DDVKRVAFHEIVPDAVKKSFANPRKISIDLVNAQQARRILDRIVGYTLSPLLGRYLER
                     GLSAGRVQSVALRLIVEREEEIENFIPKTYFVIKVEVEKDSNKINFTLTEIDGKKIEK
                     NIEEEKEVEKILEELKGGVLVVKDKKELVRKIKPLPPFITSTLQQESSIKLGFSSSKT
                     MVLAQQLYEGIDIEGKSVGLITYMRTDSPSVAKPAQNSALKFIKENIGKEYLPEKPNL
                     YKSSSKLSQEAHESIRPTYVDNTPEKIKGYLNEDQFKLYSLIWNRFIASQMKNAEVKN
                     IKVEVENGKYKFFAENNQITFDGFMKIWPIKIDTGENYLDKIEIGEELKNAEHKTEKH
                     QTKPPARYTEATLIKTLEKYGIGRPSTYAPTISTLFSRGYIKNQNRALIPLKIGRIVH
                     NVLKKFFSDIIRIDFTAKMEEDLDEIARGEKNYLDVLNEFYTSYKKILDQTYQKINES
                     DIINEIVGESRKCPHHNVNLVVKQGKYGIFLACPKFPECKYTERVKENEGNTRNRYRR
                     KQSRKVI"
     gene            11240..12610
                     /gene="argH"
                     /locus_tag="PLW95_04920"
     CDS             11240..12610
                     /gene="argH"
                     /locus_tag="PLW95_04920"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOV22007.1"
                     /translation="MKYVWEGRFGEEIDKDLIEFTSSITIDRKLALFDIEGSIAHTKM
                     LGKCNIINKEESKKIIDGLEKIKKEFQEKKFEFKQTDEDIHTAIERRLVELVGEVGKK
                     LHTARSRNDQIVLDEKLYLKSEVKKIIELITHLQNVFLKKSEENIDVIIPGYTHLQQS
                     QVILLSYYFLAFIEMFERDKERLENAYKIVDILPLGSCACCGTSLPVDRKYLAELLGF
                     SDISTNAYDTVADRDFIVDVCVACVVFLIHLSRLSEDFIIWNSQEFQFIELPDKFSTG
                     SSIMPQKKNPDIFELIRGRSSSSIGFLTGILTLMKGLPMSYNRDLQEDKTLFFPIIEQ
                     TLRSISILIKVIPEIKFNKKIIGGKISDFTLATDIAEYLVQKNIPFREAHNIVGKIVR
                     YCIENKKGLRDLDIEEFKKFSPAFTSDIKKVLDFNYSVNSKNIYGGTSLSYVKKEIEK
                     WKRKLK"
     gene            12589..13860
                     /gene="lysA"
                     /locus_tag="PLW95_04925"
     CDS             12589..13860
                     /gene="lysA"
                     /locus_tag="PLW95_04925"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOV22008.1"
                     /translation="MEKKIEIIFDKFFEYKGNKFFAEGVDIGKLAEKYKTPLYIYSLN
                     YLTKKINDFKGAFSSFPSLICYSVKANSNLSILKSIKTNGLGVDVVSEGELRRAIIAG
                     FGGDKIVFAGVGKTEEEIEFAIKNKIYCFNVENEKEIEFIEKYGKKYKRNVLYNIRLN
                     LDLDIETHHYLKTSKNETKFGLDFETGKKIIKKQKENKYSKLIGFHFHLGSQIKSSSF
                     YIKALEKISQFIKETKFYPEVIDIGGGFGIPYSPEDKVEPIKNFGEKICEYVQNMKVK
                     KIIVEPGRFIVGNTGILLTKVLYVKKRGQKTFVIVDAGMNDLIRPSLYNSSHIIVPYT
                     KKQGKEVKVDVVGPICESGDFLGKDILLPESISAGDYIIIGSCGAYGFSMSSNYNSRR
                     RPAEIIVDGEKEILIREREKFPDLWHNELCL"
     gene            complement(14046..14723)
                     /locus_tag="PLW95_04930"
     CDS             complement(14046..14723)
                     /locus_tag="PLW95_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22009.1"
                     /translation="MSKKCLGLIVGCILLVGCATGVDNVKLYQPLEYEPQQKSVVYAS
                     ASEYKPVEGNKILLALRRVSDSRPDISVIGVKKNTYGMPMGKVDVEKGKVFIDLFTQN
                     LVEVLNRAGFEVIEPQKYKILPEEKKQEAKLLLDADVRTFWVEFMPGFWAVSADSNVI
                     FEIHLFNAETNQEIWSETFRGKGHVPGLAIDRKGFEQSINMAYSEAMKSFYKVISDEN
                     FRQNILK"
     gene            complement(14782..15603)
                     /locus_tag="PLW95_04935"
     CDS             complement(14782..15603)
                     /locus_tag="PLW95_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012034065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HOV22010.1"
                     /translation="MIKKIFMKAEYYEKIKRVFEKNLKGHCFEGVDINEEIKEQIKKI
                     DEEIEVIQVEVKEMEEKRRIKDAQKIEMAKAKMDALMEKRYALLNKVPPMKFKSFCDK
                     FNIPLTFEEYKKKAEEEPILSIISVSISGSFAIEVWPYLLESGFHEEHNGKRIFVFLF
                     KDRFYIVEGTYTWEEARLLVLEDYDKERKKFERLKLLYDSEEVKEDSKSRPPIPEEVK
                     IAVWRRDNGRCVKCGSQENLEYDHIIPFAKGGSNTARNIQLLCEKCNRQKRDNLV"
     gene            complement(15909..17315)
                     /locus_tag="PLW95_04940"
     CDS             complement(15909..17315)
                     /locus_tag="PLW95_04940"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HOV22011.1"
                     /translation="MKPKVWKCFFKISLVFSALGPFLPVSYINAQTGERPVIEWEKTF
                     GGSNIDAIAFSVQQTTDGGYIMVGALLSSSTGDYDVYLIKIDEKGDEQWEKTFDSSIH
                     DIAYSVQQTTDGGYIMVGQTAYLDGSFSNVYLIKTDEKGDEQWEKTFSRTGQDVAFSV
                     QQTTDGGYIIGGGTNYYSGGSSDVYLIKTYENGDEQWEKTFGGSGIDYRGYNSAQSVQ
                     QTTDGGYILVGKTTSFGVGSYDVYLIKTDKFGNKLWDKTFGGEGLDMGYSVQQTADGG
                     YIIGGCINTDTYYDDAYLIKTDENGDEQWEKTFGREYDDCIYSVQQTTDGGYIMAGET
                     NSSLFGAAKDLDAYLIKTDENGNEQWEKVFAGSGNGEDTINSVQQTTDGGYIGAGWTR
                     LDDNKLNIWLIKIKVEPVEKGDINEDEKIDISDVILCLRQAVGLNEKDFSSADMNFDG
                     KIDIADVILVLRKAIGLE"
     gene            complement(17566..17823)
                     /locus_tag="PLW95_04945"
     CDS             complement(17566..17823)
                     /locus_tag="PLW95_04945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOV22012.1"
                     /translation="MNYEIYYKDSVKKDLKKIPKQRWEIIKNKIEKQLTTNPYQGIPL
                     KGKWEGMRKLESPPYRVIYTIIQKENTILILRIRHRKEVYR"
     gene            complement(17813..18040)
                     /locus_tag="PLW95_04950"
     CDS             complement(17813..18040)
                     /locus_tag="PLW95_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HOV22013.1"
                     /translation="MKKGYLGVRIEEEIEKRLKGLSEKTHVPKSFYVSEALRMYLEEI
                     EDYEISFERLRDKKDKVIKSEDFWREVENEL"
     gene            complement(18363..19436)
                     /locus_tag="PLW95_04955"
     CDS             complement(18363..19436)
                     /locus_tag="PLW95_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22014.1"
                     /translation="MDVSAKITGITYSPLLCRTLNEYPISQLDIAISKDGAFILILGK
                     NKQIALSWWVSPKRTRSYPYARVYDTLGFQGKKVTVIPILKDEGKDGDRDFLQWDTIS
                     LMSLLGIYTIISYYSSAEKSSGYQHKITGQKFDISHITEEIDNLINYQSDALHWNLSQ
                     IDKIDEIGKRAINFYENISKNLGVKMHSKKLAEKRINNISKGKECFISLSRDLAKKAQ
                     KRESETVQPKEKLTGKKATLTIKNYLGGYYFFTCDEVSIQRNTIYLIEGKHSKKSLIP
                     SLEDIKDGLLKMILFTNLKEVKIDKKQYNPVAVLKLTNDSVFKKEDLKQSQIDFLNLL
                     KEESKRNKFSVILNGTDLIALSL"
     gene            complement(19420..20268)
                     /locus_tag="PLW95_04960"
     CDS             complement(19420..20268)
                     /locus_tag="PLW95_04960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOV22015.1"
                     /translation="MTHHKLIIGNCMSMEEIEDESIHLMVTSPPYFNAPFDYKGLFKN
                     YEQYLGVLNRVAKEIFRVLKEGRIAVLNIDDMLVDGEKFPIVADAIKIFQNAGFRYRD
                     RIIWKKPDGYLRISRRSGVLLQNPYPMYFYPDNLLESIIIFQKGKFNYHSIPKEVREA
                     SKIDIKEFSNNKWYMTLWEMVNVLPGSSLEKEIAAFPEELPYRIIKLFSYKWETVLDP
                     FAGSGTTMKVARQLERNSIGIEIKKSLIPIIKRKVGFEGQLNFENFNDTFEVIIREKV
                     KYGCIG"
     gene            complement(20348..21295)
                     /locus_tag="PLW95_04965"
     CDS             complement(20348..21295)
                     /locus_tag="PLW95_04965"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOV22016.1"
                     /translation="MKKFFSLLLFFLFIQTGYSEFKSETRIALGTFFQITIEETKDNK
                     EILNQAFKKVDELEKKLSIFEKESEVSKLNKNKSASISNETIEVIKKAIEISKITDGA
                     FDISCKPLVDLYKKCEKSGKPPGEEEIKNTLTKVNWKNIEIKGNNISLKDGMEIDLGG
                     IAKGYIVDKAVEFLKSKGIKNGLVNAGGDLYCWGNNPSGKKWKIAIRDPFEKEKTIGK
                     IEITEKGVATSGDYEQYVKIKGQKFSHIVDPRNGKTVQDFPVSITIIADDCITADALA
                     TGFFVIGTKSIEIANKLKDVEILIIDKDKKNYKSKNFPL"
     gene            complement(21313..21678)
                     /locus_tag="PLW95_04970"
     CDS             complement(21313..21678)
                     /locus_tag="PLW95_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22017.1"
                     /translation="MKKTGLGKDPLEWIKPTVEKKIPEKELPTKRSKEIIEKQIGPKY
                     QTFDIRLTVLLKNTHLEFLDKLVREIHKNRTKEFKRERITKNTLIRCFIEAFSKTEIN
                     IKNIPDEETLFSRILEKIK"
     gene            complement(21675..22439)
                     /locus_tag="PLW95_04975"
     CDS             complement(21675..22439)
                     /locus_tag="PLW95_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOV22018.1"
                     /translation="MRKIAVINQKGGSGKTTSVVNIGWCLSENKRKVLLIDLDPQAHT
                     TIHLGFEPPNIEKSIYEVLINEVPIDETIVQINNYLYLVPSKIELASAEIELVTAVGR
                     EIILKEILKKYKKRFDYILIDCPPSLGLLTLNALTTVNEVFIPIQAEFFALEGLTKLL
                     QTIQLVKERLNPSLEISGIILTMFDMRKNICKDVADKVKNHFNEKIFKTFIRENVRLA
                     EAPSFGKSIYQFSPDSHGASDYRKLTKEIIAQEKKK"
     gene            complement(22585..23871)
                     /gene="aroA"
                     /locus_tag="PLW95_04980"
     CDS             complement(22585..23871)
                     /gene="aroA"
                     /locus_tag="PLW95_04980"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOV22019.1"
                     /translation="MDVRVKRVENIEGEIEVPGDKSISHRSLIFGAISEGTTEIKNLL
                     ESSDCFSTLECLEKSGIKIEKGKTYKIYGNTFREPEDILDCGNSGTTIRLLSGVLSSK
                     NFLSILTGDNSLKQRPMKRIIEPLEKMGTLIIGRENNSFPPIVIKGRKLNGIDYKIPV
                     SSAQVKSCIILASLFSDGETTIEEPYQTRDHTERMLQYFGGEIIKEGNKIHIPGNQKL
                     YGRNVIIPGDFSSASYFIAIGLLIKNSKIRIKNINLNPTRTYFLKVIEKMGGEIKITD
                     QKKVCNEEVGTIEIEYKGKLNGIEIGEKEVPLIIDEIPLIGVIASVSNGKTIISGAKE
                     LRYKESDRIKAIVSQLKKMGSEIYEKEDGFEIHGVEKLKGNNVNSWNDHRIAMCLTIA
                     GLIADGETKIENTDCVKISFPQFYDLLSSIKIPISQ"
     gene            complement(24316..>25293)
                     /gene="aroF"
                     /locus_tag="PLW95_04985"
     CDS             complement(24316..>25293)
                     /gene="aroF"
                     /locus_tag="PLW95_04985"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOV22020.1"
                     /translation="NKIKNMGFNVNISKGTEKTIIGIIGDTRGISDEVFKIMPGVIEV
                     ISILRDYKLASREFHPHNTIIKVNSIKIGEGTITIIAGPCSIESKSQLLSTAKHIKSK
                     GAHILRGAAYKPRTSPYAFLGLGKEGLEILKDIKEEVKIPIITEVLSIDEIEEIAEVA
                     DILQIGARNMYNYPLLKKVGEIDKPILLKRSFSATIKEFLSCAEYILKEGNQNVILCE
                     RGIRSFDNDFTRNTLDLSAIPVLKKETHLPVIVDPSHGTGRRDIIIPMSKAAIAAGAD
                     GLMIEVHPKPEEALSDGFQSLTFDLFGKLIDEITPIIKVIGKKIPSFPL"
BASE COUNT         7544 a         4401 c         3194 g        10154 t
ORIGIN      
        1 gctcctacac ctacaaacat ttcaacaaaa tcagaaccac tcatgcttaa aaatgggacc
       61 tttgcttctc ctgcaactgc ttttgcaaga agtgtttttc ctgttccagg agcaccaatt
      121 aaaagtactc cccttggtat ttttgctcct aatctgacga acttttttgg atttttaaga
      181 aattcaatta tttcttttaa gtcctctttt gcttcttcac atccagcaac atcatcaaat
      241 gtaattctgt ttttattatc aggcattatt ggtctacttt tggcaaatga cataacacga
      301 tttccctcag ttgagacctg tttaaaaagt ataaaccaca taagaagaaa aagaaagata
      361 atagggataa ctgaaataaa aagattggac caaaatgaag aacttggttc tggggcgaaa
      421 gaaacattat tttctctcaa aattttataa agttcattat cttctccaca ataagtataa
      481 aattttgttc catcttttaa ttctccgtaa atatacccat tttttatttt caaagtacca
      541 ttgatatttt gactttccac ttgttttata aaatcactat aaattaaatt cttttttgcg
      601 ccttcatatt cagaaaatct caataatgaa aataaaataa tacctataat tatccagaaa
      661 attagtcctt taaagatatt gtttgtgggc acttttattt gttgtttttt atttttgttt
      721 ttttgtttat tttcatccat tcttttttca ccttcttttt tatttgtttt aaatattctt
      781 ctttgttttt tgctttttct attgcagata tcaaagcaac tccgtctgct ccatttttta
      841 ttacttcctt tacattatct ttatttatac ctccaattgc cactaatgga atatttattt
      901 tcttttttat atcctttata attgtagtac ctactacttt tctttctttt tttgttgggg
      961 atggataaat tgctccaatt gctaaataat caatatcctt ccccttatat tttaaaactt
     1021 cttctaatga attcgttgaa acccctataa ttttgtccgg tataactttt cttgcatcct
     1081 caccacttat atctctatct cctaaatgaa ctccatctgc atcaacaatt tcacatatat
     1141 caactctatc atttataatg tatattatct catctggtat aacttttttc aatttttttg
     1201 ctatatctaa gaaatatttt atattttctc ctttatatct taattgaatt atatcaactt
     1261 ttccttttat tatttcctct gtaatgctaa aaatattttt attatcctct aaatttataa
     1321 ttgcataaac aaatggaaga agtatttttt ttcttgaaat taaagttgta atttgttttt
     1381 ctaaattgta aaactcaaac cttaaatcat gaaatatact tgaaatttta ccatcaatac
     1441 actttgaata ttcttcaatt gccctcagcc cttcttcaac ccttaaacaa tttttctcta
     1501 ttattctttt tatattttct ttttctttta catcataatt ctttccaaca tcataagaac
     1561 tttctcttgt tttatgtaag aaaataattg ggaagttttt aattacttcc tttataaatt
     1621 tatgtctgaa atcttttatt ttcttaaaaa tttcttcatt ttctaaataa aatcttattc
     1681 catcttctat aactctcaag ccctctcttg ttctattaaa atttgcatca attattctaa
     1741 ctcttttgtc catttttaat ttttttaatt atatttgttg ttgatatatt tccaataaga
     1801 ggaaaaatat agaccttacc accatacttt tcaacaatat ctttacctac tacatcttct
     1861 tttttataat cccctccttt tattaaaata tctggtttta ttatttttat tagtttttca
     1921 ggagtagttt gaccaaaagg aacaacataa tcaacaaatt ctattgcaga aataatttca
     1981 atacgctgat tttcatttaa aataggtcta tttttacctt ttattttttt aattgaacta
     2041 tctttgttta ttccaactat aacaacatct ccaagttgtt ttgctttttt tagaagtttt
     2101 atatgccctg aatgtataat atcaaaacac ccatttgtaa aaacaatttt cttcttttcc
     2161 ctttttaatt catttactat tttgactatt tccttaaaac tctttttctt tctcattatt
     2221 caacaagaga acaaataata tgataaatta ggcagtggac ttcctgaatt cttggtgtgt
     2281 cttcggaagg gactgataaa gtaagagatg aaatttttga taaagaattt ggttctttac
     2341 ctgtgaagga aattatttcc attccaactt ttttcccttt atgagcagca agtaatatat
     2401 tttctgattc tcctgatgta gagaaacata taagtatatc tcctttttca ccaagacatt
     2461 ctacctgtct ttcaaatatt tttttaaaag aatagtcatt acttatgctt gttaatatag
     2521 atgtatctgt tgtaagagaa ataaatggat atggttttct ttctttttta aatcttccaa
     2581 ccatttcacc tgcaaaatgc tggcaatctg ctgcacttcc accatttcca cataaaagaa
     2641 cttttttctt attttctaaa cattttttta tcattaaggc agcagacaat atcctgtcaa
     2701 tattttcttt ttcaagaagt tcttttttta gttttatact ttcttctatt tcctttttta
     2761 ttttctcaat attattgttc tggttcatct tctgtttgtt caggtatttt ttcatttaaa
     2821 aattcttctt ttgatatatt ggttacatat acttcaatat ttgaaactct ctcaacccca
     2881 acagcatcct gtaaatagtt ctttgttatt tcctggattg tagcacatgt ttctggtata
     2941 ttatgtccag aagttaattt tagataaaac tttgtttcaa gcccttttgg agaaacataa
     3001 tttttaactc tcaatgattt gataccatct atttgtttag atattaatgc agtaatagag
     3061 tcctcaattg cttttaaaga gattttcacc tctccaccgg gattatcaaa agaaatattt
     3121 tttgttttat ttatatattc aaaccagttt acaatatata taactcctaa aataaccagt
     3181 accatcccaa ctgcaccaat tgctgctaca ttctttttta aaaaggtaga aagaatatca
     3241 acatagtaag gattcgccca gataataagt aaaaaaatag aaaagataat acctgcaaga
     3301 atataaatag cactaacaat tctgccaaat attttcattt ttttcctcca attattataa
     3361 attataccac aaaacaaaac cacagaataa aacccttaca agtttataat aattcataat
     3421 ttttcccagg aagttctttt ataattcctt ttaattctaa tttaagcaag attgttgaaa
     3481 ggcagggata gtccatacct gttaatttta aaagttgttc aatgttgact ttttcattta
     3541 taaaagacaa aaccttcttt tcttcttcat caatgttttt tactattttt tcttttttct
     3601 cttgttcttt tatttcaata ttcagttctt ccaatatatc tgaaatgtcc tgtataagta
     3661 ttgctccatc tttaagaagt ttatttgttc cttttgaagt tatgctatct gcctgtccag
     3721 gtagtgcgaa tacatctctt ccctcggaaa tagcaatgtt tgctgtaatt attgctcctg
     3781 accttggtcc agcttcaaca acaattactc cttttgaaat gcctgcaatt attctgtttc
     3841 tgattggaaa attccatttt tcaggtaata cactggaagg aaattctgtt ataattgctc
     3901 ctcctaattc tacaatttta tcaacaagtt ttatattttc ttttggataa aaattgtcaa
     3961 atccggaacc taaaactcca actgttttcc ctttactttt tattgctcca taatgacaaa
     4021 tataatcaat ccctcttgca agaccgctaa caattgtaat gcccagtgat gaaagttgaa
     4081 aggaaaattt ttctgccatt ttaattccat aataagatgg attccttgtt ccaaccattg
     4141 ctattggaaa tgggtgattt tctatttccc cttttacata aagaatataa ggtgggtcat
     4201 atatctgttt taataatggg ggatattctt tatcttttat tgttattatt ttaatgtcat
     4261 tttttatagc atattcaact tctttttccc agttaattgt ttttatgttt tttatttttt
     4321 ctgctgattt ctggtcaata ccttttttta ctaactcatc tactggtaaa ttaaataaat
     4381 tttcaatatt ctctgttttt tcaataaatt tatataactt tgaaggtttg atttttaata
     4441 tagaagcacc aatccagtat tttttatctt ccattttata caagtgattc aagattaaaa
     4501 agtttttgat attcaagttg tgcaaatcta tctgtcatac ctgcaatata gtcacatatc
     4561 acaatttctt ttggttcata ttccattttc ttctggatat ggggaggtag ttgttttggt
     4621 tcatttagat atgcattaaa aaggtcagta ataattcttt ctgctttttt tgccatcctt
     4681 attactctgt aattttcata catattttct tcaaggaatt ttcttaattg agtatgtttt
     4741 cttattactt cttcagaatg tgtaataaga atatgggtat ttcttacatc ttcaatagaa
     4801 gaaacaccgg atttttcaat gttctctata ctctggcaaa taacatcact cacaagataa
     4861 tttatgaaat ttcttatcat tacatgcctt ttgtgttttt caggtatttt ttcaatattt
     4921 acttttttct ctatttcttt ccaaagagtt attttttcaa tttccttaaa acttataatc
     4981 ccagacctta ttccatcatc aaggtcatga caagtatatg caatttcgtc cgatacatta
     5041 acaacctgag cttcaagtga aggagaggtg aattttgtga attcctgata ttcatctatc
     5101 aggggtctgt cataacttgt tttatgcctt attattccct ctcttacttc ataggttaaa
     5161 tttaaaccag gaaaatcagg atatctttct tctattttat caacaacttt taatccttgt
     5221 tcattatgtt caaaaccagg aaatttatgt tctttcataa gtttatcaag tactaattct
     5281 cctttatgac caaaaggagt atgtccaatg tcatgagcaa gagaaatacc ttcaacaagg
     5341 tcttcattta atttcagcgt tcttgctatt gttcttgcaa tttgctgtac ttcaagagta
     5401 tgcgtaagtc tattcctgta atggtcacct tctccataga taaaaacctg tgttttatat
     5461 tcaagtcgtc tgaatgcagt agaatgaatt attctatctc tatctctttg aaaaacattt
     5521 ctgtatggat gttcttcttc tttatatttt ctccctaatg aagaagaact tcttgctgca
     5581 tatggagata ataaaatatc attatcttta atatcctgat tatttttcat ttttctggta
     5641 agtaaattaa agggtcaact gccttatcac ctattcttat ttcaaaatga agatatcttt
     5701 ttttctgtgt tccccctttt atttttccta ttacctgtcc cttttttact ttttctcctt
     5761 tttttgttag aatttcaagg tcatgtccat aaatagtata aagattattt gtgtgttcta
     5821 ttataattgt ttcctgatat tttgctgttg ttccgacata acttactgtc cctgctgctg
     5881 aagaaaaaac tgtacttcct tgttcaacta ttatatcaat tccctgattt cccagttctc
     5941 catatttaca tataatatct cccttttcta ctggccatat aaaggcacta tcttttataa
     6001 ttgggatgta ttcatttttt gtttctactt catttctatc agaagatggt tgttttatat
     6061 ttgatgaagg gggaagaaga gcatatgaag gttgtttttt atttgttcct ggtattttaa
     6121 gtttttgccc acaggatata ttgtcactaa ataagttatt ggcttttttt attttttcaa
     6181 cacctacttt tatttcagat gtactttttc catcataata atactctgaa attctaaaaa
     6241 ggttttcacc actttttaca tagtgatagg tataatttgg ttcacttttt ttctttgaag
     6301 tactggttcc cacagtaaat gtacaaccag aaattaaaaa tattataccg ataatttcaa
     6361 tttctttata aaatttttta accagttttt gaaaatttat cattttctcc ctttaattaa
     6421 attatactat tatctatcaa tataggttaa acaaagatta cttattattt tccttaaaaa
     6481 aattacagaa attttttatt acacatttca gacattctgg attttttgct ttacatacat
     6541 ttctcccatg taaaattaat ctaaatggaa aatcaatcca attttttttg ggaataattt
     6601 tcataaggtc ctgttcaatt tttgatggct ctgtattttt tgtaagtcca agtcgttgag
     6661 agacacgctt aacatgagta tcaacaacaa ttccctcact tttcccgaat gcctgggaaa
     6721 caactacatt tgctgttttc cttgcaactc ctggcaattt aattaattcc tctactttat
     6781 ctggtacttt cccgttatat tgctttacaa gaatttcaca taaatttttt atattttttg
     6841 ctttattttt ataaaaattc actgaactta taattttttc aatttcttca actggacttg
     6901 aggcaaaatc atatggggac ttaaatttct caaaaagtat tggagttatt ttatttacca
     6961 atttgtctgt tgtttgtgct gaaagaattg tggcaataag aagttgaaat ggattctgaa
     7021 aaacaagaga ggttttactt tctggatatt ctttttcaag tatccttatt attttattaa
     7081 cattttcttt ttccatataa cctattttat caaaaaaata aaatttttaa aaattgtcag
     7141 tagattttaa atttattaat aagatacaaa tagttaatat aggaaaaaat taaagatgat
     7201 atttgttaaa acaataccat ttttttattt tgtaacccta aaatcctgtt ttcttataac
     7261 tatggagaat tgaaaattat caaaaagaaa aagtagtgta gatttagaat tgtgaatttg
     7321 aataatagat gtatttatat tataactatg aaaaaattta aagaattcac attttgaaag
     7381 gggattatac tatttttaca tcaaaggtgt aataaaaatt aacatccccc tcctcactta
     7441 tatccctcgg gcgtagttca taatccgtag ccaaaagcga ggacgctacg gtgcagaatg
     7501 agggggattg ttttttcttc tcccctctgg ggagaagatt atgatgaggg ggagaaaaaa
     7561 gcaaaaagcc cccctcaccc ccgccctctc ccccgagggg agaggatagg tgaggggaaa
     7621 atatatactc tatgtatgtt gtcaaattgg tattaactgc atagttatta aaatttgcgg
     7681 gattttaggt ttgtaacata aacctaaaaa ctttttctct tttagtcaaa ttacttattc
     7741 tatcaattat gctctttcat tttttttatc ttgttactta ctactttctg gttaatcttg
     7801 ttgagttgaa ctatattttt attttttctg catattttcc tattacaggg agtggctctg
     7861 tcttttcact gagagcatta actatcccta ttatcattaa taccagtaaa aatatcccaa
     7921 caaccatacc tacgacccat cctatcactg gtattatcca caaaattctt gttattatgc
     7981 cacaaatcaa caataccaac ccttgtttta catgaaactt tgagtactcg ttatccttat
     8041 tcacaagaag cggtatcaaa agaagaagtc ctaagtatga aagccatgct aatccctttc
     8101 cttcatcaat tactttctta tccattgtcc ctcctatttt gtttatataa ccgcggggtt
     8161 aaatttacgc ggttgaacaa aatttatctc ttaatcctgg aaagttgaaa ttactttttt
     8221 aatgtcaatt gttctgtcat aaattgcttt tcctataaca actccatcaa ttttatatcc
     8281 aattgaattt aaagcaatta tatcattttc atcttttact ccgccagaaa taattatttc
     8341 ttctattttt tctcttatgt tgtctatttt tattaaaact cttccaatag gcacaatatc
     8401 aataccttca agtgtcccat ccctttcaat atttgtaatt aaaaatctac taaatccctt
     8461 ttcaataaat ttttgtatca aaaattcagg caaaattgga actgttacat tccatccact
     8521 caatccaatt tcattattta taaaatcaat agcaacaatt atcctttttt taatatctct
     8581 gttaagtttt tcaattattt caatttcagg agattctttt tcaactttaa agtcctttcc
     8641 tgcttgttga taaagaaatc tcaagtcctg tatagtatat ccattttcaa gtaaaattgg
     8701 aactaaaaga gaagtaccca ttattacaaa atcaatacca gttgaaagat agtgtctaac
     8761 ctgacctggt tttcttattc ctcctcctaa ttgtatttca cattttggat ttaatctgtt
     8821 ttttgtttct atgacttctt ctataatttt ttgatttcct tttgagtggt gtcctttttt
     8881 tgcccctgaa agcataataa gatgtattct ttttgctcca aattcaataa atttttttgt
     8941 aaaaaaagct ggagtgtctt tgtaaaaagt taccctatta taatttcctt tatataacct
     9001 tgcgactttg ttttcttcta tatctattgc gggtattacc ttcattttct tttacccttt
     9061 cagtatattt acactcgggg aatttcggac acgcaagaaa aattccatat ttcccctgtt
     9121 ttactactaa attaacatta tgatgtgggc attttctact ttctcccaca atttcattta
     9181 ttatatcact ttcatttatt ttttggtatg tctggtccaa tatcttttta tatgatgtat
     9241 aaaattcatt taaaacatca agataatttt tttctcctct tgcaatttca tcaaggtctt
     9301 cttccatttt tgctgtaaaa tcaattctta taatatctga gaaaaatttt tttaatacat
     9361 tgtgaacaat tcttccaatt tttaaaggaa ttaatgctct attctgattt tttatatatc
     9421 cccttgaaaa taaagttgat attgttggtg cataagttga gggtctgcca attccatatt
     9481 tttccaatgt ttttattaaa gttgcttctg tatatcttgc aggtggtttt gtctgatgtt
     9541 tttctgtttt atgttctgcg ttttttaatt cctcaccaat ttcaattttg tcaagataat
     9601 tttctcctgt atcaattttt ataggccata ttttcataaa tccatcaaaa gttatttgat
     9661 tattttcagc aaaaaattta tattttccat tttcaacttc aacttttata ttcttaactt
     9721 ctgcattttt catctgactt gcaataaacc tattccagat aagagaatat agtttaaact
     9781 ggtcttcgtt taaataccct tttatttttt ctggtgtatt atctacataa gttggtctta
     9841 tgctttcatg tgcttcctgt gaaagttttg aactactttt atataaattg ggcttttctg
     9901 gcagatattc ttttccaata ttttctttta tgaatttcaa tgcagaattt tgtgctggtt
     9961 ttgcaacaga aggagaatca gttctcatat aagttataag cccaacactt tttccttcta
    10021 tatcaatacc ctcatataat tgttgtgcta aaaccattgt ttttgatgaa gaaaatccca
    10081 attttattga actttcttgt tgtaatgtac ttgttataaa aggaggtaat ggctttatct
    10141 ttcttaccag ttctttttta tcttttacaa ctaatactcc accttttaat tcttctaaaa
    10201 ttttctcaac ttctttttcc tcttcaatat ttttctcaat tttctttcca tctatttcag
    10261 ttaaagtaaa attgatttta ttgctatctt tttcaacttc aactttaata acaaaatatg
    10321 ttttaggaat aaaattttct atttcttctt ctctctcaac tatgagtcgt aaagcaactg
    10381 attgaactct tcctgctgat aaccctcttt ctaaatatct tccaagaagt ggacttaaag
    10441 tgtatccaac aattctatca agaattcttc ttgcctgttg agcatttaca aggtcaattg
    10501 atattttcct tgggttagca aaagattttt ttactgcatc aggaactatt tcgtgaaaag
    10561 caactctctt aacatcatct atttctttac ctatggcaat tgttgtatgc catgcaattg
    10621 cttctccttc tctatcttca tcagttgcca taaaaatttc ttctatgtct tcagtcaatt
    10681 tctttatttt tgaaacaatt tttctttttc ccggtgagat aacataagtt ggttcaaaat
    10741 ttttttcaat attcacacca aatttatttt ttggtaagtc cctcacatgt cccatagttg
    10801 cagtgattgt atattcctta cctaaaattc cacttattgt ttttgctttt gttggtgatt
    10861 ctactattat tactcctttt ttcattcttt atttcctttt ctttttaagc ccgggtactg
    10921 attcctttat ggtggactgt attatagaac aatcctttaa aaaatcctcc caattcccta
    10981 tatttcctta atcccccttc atccccgttg tgctatctgg gagacgatga cgcaccaact
    11041 ctgctttgct actctttgct tctttagtcg gtttctaaaa gaggggaatg acgcaccaac
    11101 tctactttgc taccttttgc ttccccagtc ggtttattaa aggggggtag gggggattta
    11161 tccttttccc tttgcctttt ttaaaaatat atttacatat tataatacat ctgataaaat
    11221 aaaaaaatca aggggaaaaa tgaaatatgt ttgggaagga cgatttggag aagaaattga
    11281 taaggacttg attgagttca catcatcaat taccattgat aggaaacttg ctctttttga
    11341 tattgaagga agtatcgctc atacaaaaat gcttggaaaa tgcaatatca taaataaaga
    11401 agagagcaaa aaaattattg atggattgga aaaaataaaa aaggaatttc aagagaaaaa
    11461 atttgaattt aaacaaacag atgaggatat tcatacagca attgaaagaa gattagttga
    11521 actggttgga gaagttggaa agaaattaca tactgcaagg tcaagaaatg accagattgt
    11581 tttagatgaa aaactttatt taaaaagcga agtaaaaaaa ataatagaac tgattaccca
    11641 tttgcaaaat gtctttttga aaaaaagtga agaaaatata gatgtgatta tccccgggta
    11701 tacacatcta caacagagtc aggttatact tctttcttat tattttcttg cttttattga
    11761 gatgtttgaa agagataaag agagattaga aaacgcatat aaaattgttg atattttacc
    11821 acttggaagt tgtgcctgtt gtggaacttc tttaccagtt gacagaaaat atcttgctga
    11881 acttcttggt ttttccgata tttcaacaaa tgcttatgat acagttgctg acagggactt
    11941 tattgttgat gtttgtgttg cctgtgttgt ttttcttatt catctttcaa gattatcaga
    12001 ggactttata atatggaata gccaggaatt tcaatttatt gaattacctg ataaattttc
    12061 aactggttca agtataatgc ctcagaaaaa aaatccagac atatttgaac ttataagagg
    12121 caggagttct tcttcaattg gttttttaac agggattctt acattgatga aaggacttcc
    12181 aatgtcttac aacagggatt tacaggagga taaaactctt ttcttcccta taattgaaca
    12241 aacattaagg tcaatttcta ttttaataaa agttattcca gaaataaaat ttaataaaaa
    12301 aataatagga gggaaaatat ctgactttac acttgcaact gatattgctg aatatcttgt
    12361 ccagaaaaat atccctttta gagaagcaca taatattgtt ggaaaaattg tcaggtattg
    12421 tatagaaaac aaaaaagggt taagggattt agacattgaa gaatttaaaa aattttcacc
    12481 tgcttttact tctgatataa aaaaagttct tgattttaat tattctgtta attcaaaaaa
    12541 tatctatggc ggaacttctt tgtcttatgt aaaaaaagaa atagaaaaat ggaaaagaaa
    12601 attgaaataa tttttgacaa attttttgaa tataaaggaa acaaattttt tgcagaagga
    12661 gttgatattg gtaaacttgc tgaaaaatat aaaactccat tatacattta tagtttaaat
    12721 taccttacca aaaaaataaa tgattttaag ggtgcttttt cttcttttcc ttctcttatc
    12781 tgttattcag taaaagcaaa ttcaaattta agcattttga aatccataaa aacaaatggt
    12841 ttaggagttg atgttgtttc agaaggagaa ttaagaaggg caataattgc tggttttggt
    12901 ggagataaaa ttgtatttgc aggtgttggt aaaacagaag aagagattga atttgcaatt
    12961 aaaaataaaa tttattgttt taatgttgaa aatgaaaaag aaattgaatt tattgaaaaa
    13021 tatgggaaaa aatacaaaag aaatgtttta tataacataa gattaaatct tgaccttgat
    13081 attgaaacac accactactt aaaaacatca aaaaatgaga caaagtttgg tctggatttt
    13141 gagacaggga aaaaaattat aaaaaagcag aaagaaaata agtattctaa actcattggt
    13201 ttccatttcc atttaggctc tcaaattaaa agttcatcat tttacataaa ggcgcttgaa
    13261 aaaatttctc aatttattaa agaaacaaaa ttttatccag aggtaataga tataggaggt
    13321 ggatttggga ttccttattc accagaggat aaagttgagc caataaaaaa tttcggagaa
    13381 aaaatatgtg aatatgtcca gaatatgaaa gtaaaaaaaa ttattgttga accaggaaga
    13441 tttattgttg gaaatactgg tatcttatta acaaaggttt tatatgtaaa aaaaagagga
    13501 caaaaaacat ttgttattgt tgatgctgga atgaacgacc ttataagacc ttctctttat
    13561 aatagttctc atataattgt cccttataca aaaaaacaag ggaaagaagt aaaagttgat
    13621 gttgtaggac ctatttgtga aagtggggat tttttaggaa aagatatact tttgcctgaa
    13681 agtataagtg caggagatta cataattatt ggtagttgtg gtgcctatgg gttcagtatg
    13741 tcctcaaatt ataatagcag gagaagacca gcagaaatta ttgttgatgg tgaaaaagaa
    13801 attttaatca gggaaagaga aaaattccct gacctctggc acaacgaact ttgtctctga
    13861 taacaatttt tatattctat tttcaaaaat tcattttatt cttttttatt actcttcttt
    13921 gtaaaggata tatttcttac tcaatccttt tccctttaac agggtagggg gatttagtaa
    13981 agcacaacgg cggtttatta ttttttatta ctattaaata ccgcaatcta aaaatccctt
    14041 gttgtttatt ttagaatatt ttgccgaaaa ttctcgtcgg aaataacttt gtaaaaactt
    14101 ttcatagctt ctgaataagc catatttata ctttgttcaa atccttttct gtctattgcc
    14161 agtccaggaa catgtccttt accgcgaaag gtctcgctcc aaatttcctg attagtttct
    14221 gcattgaaaa gatggatttc aaaaataaca ttactgtcag cagaaacagc ccaaaatcct
    14281 ggcatgaatt ctacccagaa agttcttaca tctgcgtcta aaagtaattt tgcttcctgt
    14341 tttttctcct caggtaaaat tttatatttt tgtggttcta taacttcaaa tcctgcacga
    14401 ttaagtactt ccacaagatt ttgagtgaat aaatcaataa acactttacc tttctcaaca
    14461 tctacttttc ccataggcat accataagta ttttttttga caccaatgac agatatatca
    14521 ggacgggaat cacttacccg tcttaaagcc aataaaattt tgttcccttc tacaggttta
    14581 tattcagaag cagaagcata taccactgat ttttgttgcg gttcatattc aaggggttga
    14641 tataatttca cattatctac gcctgtagca catcctacta ataaaataca ccctactatt
    14701 aaccccaaac atttttttga catattttcc ccctttagcg gtactgccaa aattggctat
    14761 tttcacgaat acattctggc ttcaaactaa attatctctt ttttgacgat tacatttttc
    14821 acaaagcaat tgaatatttc tggctgtgtt gcttcctcct tttgcaaaag gaattatatg
    14881 gtcatattct aaattttctt gacttccaca ttttacgcat ctcccattat ctcgtctcca
    14941 gacagcaatt tttacttctt ctgggatggg tggtctggat tttgaatctt ctttgacttc
    15001 ttctgaatca taaagtaatt ttaatctttc aaattttttt ctttctttat cataatcttc
    15061 taatactaat aaccttgctt cttcccaggt gtatgttcct tcaactatat aaaagcggtc
    15121 tttaaaaaga aaaacaaata ttctctttcc gttatgttct tcatgaaacc cactttctaa
    15181 taaatatggc cacacctcta tcgcaaacga tccactaatg cttacagaaa ttattgaaag
    15241 aatgggttct tcttctgcct tctttttata ttcttcaaag gtcaaaggaa tattaaattt
    15301 atcgcaaaaa ctcttgaatt tcattggagg aaccttgttg agaagagcat atcttttttc
    15361 cataagagca tccatttttg cttttgccat ctctattttc tgagcatctt taattcttct
    15421 tttttcttcc atttctttaa cttccacctg tattacctct atttcctcat ctattttttt
    15481 aatttgctcc tttatttctt catttatatc tactccttca aaacagtgtc cttttagatt
    15541 tttttcaaaa acccgtttta tcttttcata atactctgct ttcataaata tttttttaat
    15601 catttttcat attcctttta gttctctcaa ccctccactt ctgtctctat cttcactgct
    15661 tcttcctctg tcaatgttag aatagaaaag ggttattaga tttcacccac caaatttagt
    15721 tatcaaaatt attgtaaaac ataccttaaa gaagtcaaat tttggtattg tctcccttgt
    15781 tcaaccccgc ggttgaatat attcaggggg tattttatat cagatgagta tggtttagtt
    15841 cccaaaatac tccttttgga aactgaaaaa ggaagaataa gataaaactg ctttttgtta
    15901 tactggaatc actccaggcc aattgctttc cttaaaacta aaattacatc tgcaatatct
    15961 atctttccat caaagttcat atcagcggaa gaaaaatcct tttcattaag tccaactgct
    16021 tgtctcaaac ataaaattac atccgaaata tctattttct catcctcgtt tatatcgcct
    16081 ttttctactg gttccacttt tatttttatt aaccatatat tcaatttatt atcatcaagc
    16141 cgagtccacc ctgcccctat atatccacca tctgttgttt gttgcactga atttatggta
    16201 tcctcgccat ttccacttcc ggcaaatacc ttttcccatt gttcatttcc attttcatcc
    16261 gttttaatca gataagcatc caggtccttt gcagcaccaa ataaagaaga gttggtttca
    16321 cccgccatta tatatccgcc atcagttgtt tgttgcactg aatatataca atcatcatac
    16381 tccctgccaa atgttttttc ccattgttca tctccatttt catccgtttt aatcagataa
    16441 gcatcatcgt aataagtatc agtattgata caacctccaa ttatataccc cccatcagcc
    16501 gtttgttgca ctgagtatcc catatccaga ccctctccac caaatgtctt atcccacagt
    16561 ttatttccaa atttatccgt tttaatcagg tagacatcat aagaaccaac accaaaagat
    16621 gtcgtcttcc cgaccagtat atatccacca tcagttgttt gttgcactga ctgtgcacta
    16681 ttataaccac gataatcaat accacttcca ccaaatgtct tctcccattg ttcatctcca
    16741 ttttcatacg ttttaatcag ataaacatca gaagaaccac cactataata attcgttcct
    16801 cccccaatta tatatccgcc atcagttgtt tgttgcactg aaaatgctac atcctgccca
    16861 gtacggctaa atgttttttc ccattgttca tctccctttt catccgtttt aatcagataa
    16921 acattagaaa aactaccatc tagataagca gtttgcccga ccattatata tccgccatca
    16981 gttgtttgtt gcactgaata cgctatatca tgaatactgc tgtcaaatgt tttttcccat
    17041 tgttcatctc ccttttcatc tattttaatc aagtagacat cataatcacc agtgctagaa
    17101 gagagaagtg cccctaccat tatataccca ccatccgtcg tttgttgcac tgaaaatgct
    17161 atagcatcta tattacttcc accaaatgtc ttttcccatt caatcactgg tctttcaccg
    17221 gtttgagcat tgatgtaact taccgggaga aaaggtccca gcgctgaaaa aacaagagat
    17281 attttgaaaa aacatttcca aacttttggc ttcatttgtg cctccttttt aacttcctca
    17341 ccacttcacc tctgcctccg cttcaatctt cactgcttct tcttaaaaat ttgtcttttt
    17401 tatattcaat ctataattat taatctttag ggttatttta gattttagcc accaaattta
    17461 gttatcaaaa ttattgtaaa acatacctta aagaagtcaa attttgctat tgtttccctt
    17521 attcaacccc cgcggttaga agagatatat tgaaaaattt atcaattatc tgtaaacttc
    17581 ttttctatgt cttattctta aaataagtat agtgttttct ttttgaataa ttgtataaat
    17641 tactctataa ggtgggcttt ctaattttct cataccttcc cattttcctt ttaggggtat
    17701 tccttgataa ggatttgtag ttaattgttt ttctatttta ttttttatga tttcccatcg
    17761 ttgtttagga atttttttga ggtctttttt tacactatct ttataataaa tttcataatt
    17821 cattctcaac ttctctccag aaatcttctg attttataac tttatctttt ttatctctta
    17881 acctctcaaa agatatttca taatcttcta tctcttctaa atacatcctt aatgcctcac
    17941 ttacataaaa acttttagga acatgtgttt tttcacttaa tccttttagt cgcttttcta
    18001 tttcttcttc tattcttaca cctaaatatc cttttttcat ttttcatttc tcctctttct
    18061 gttttatacc atttttacat caaggatata ataatgctta caaagaatcc cccacttcat
    18121 ccttgctcat agggggggag gctcgccccg cagtgtcctc gcttttggct atgaatccca
    18181 tcagggcgta gcccctacgg gcgagcctct ggactacgcc cgagggaaaa agatgatgac
    18241 gaaatgtttt actatatatc cgatgttaaa tttgttttac attataccat ttatttcttc
    18301 aagagcaata aaaaaatttt ttattcaacc cccgcgcggt tagatatggt aaattatttt
    18361 ctttataatg atagtgctat taaatctgtc ccatttaata tgacactaaa cttgtttctt
    18421 ttagattctt ctttcaatag gtttaaaaag tcaatctggg attgtttcaa atcttctttt
    18481 ttaaataccg agtcattggt aagttttaaa actgcaactg gattatattg ttttttatct
    18541 atcttcactt ctttaagatt ggtaaataaa atcattttca ataacccatc ttttatgtct
    18601 tctaatgaag ggattaaact ttttttgcta tgtttccctt caattaaata aattgtattt
    18661 ctttgaatag atacttcatc gcaggtaaaa aaatagtatc ctccaagata gttttttata
    18721 gttagagtcg cttttttacc tgtcaatttt tcctttggtt gaacagtttc actttctctt
    18781 ttttgtgcct tttttgccag atctcttgat agagagataa aacattcttt tccttttgag
    18841 atattattta ttcttttttc tgcaagtttt tttgagtgca ttttaactcc aaggtttttt
    18901 gaaatatttt cataaaaatt aattgctctt tttccaattt catctatttt atcaatttgc
    18961 gaaagattcc aatgtaacgc gtcggactga taatttatta aattatctat ttcttccgta
    19021 atatgcgata tatcaaattt ttgccctgtt atcttgtgtt ggtatcctga acttttttct
    19081 gcactactat aataagaaat aattgtataa attcccaata aactcattaa agaaattgta
    19141 tcccactgta aaaaatccct atctccatct ttgccttcat cttttagaat tggaattact
    19201 gttacttttt tcccttgaaa tcctaatgtg tcatagaccc ttgcataagg gtatgacctt
    19261 gttcttttgg gagaaaccca ccaacttaat gctatttgtt tattttttcc aagtatcaaa
    19321 ataaatgctc catcttttga aatagcaata tctaattgtg aaattggata ttcatttagt
    19381 gtgcgacata aaagagggga atatgtaata ccagttattt tagccgatac atccatattt
    19441 aaccttttct cttattatta cttcaaatgt atcattaaaa ttttcaaaat ttaattgtcc
    19501 ttcaaaacca acttttctct ttataattgg aattaaggac ttttttattt ctattccaat
    19561 gctgtttctt tcaagttgtc ttgctacttt catagtagtt ccacttccag caaatgggtc
    19621 taaaacagtt tcccatttat atgaaaataa ctttattatt ctataaggta gttcttccgg
    19681 gaatgcagca atttcttttt ctaatgatga gccaggaaga acattaacca tttcccataa
    19741 ggtcatatac catttattat ttgagaactc ttttatatct atttttgatg cttctcttac
    19801 ttcttttgga attgaatgat aattaaattt tcctttttga aaaataatta tgctttctaa
    19861 gagattatct ggataaaaat acattgggta tggattttgt aaaagaactc cacttcttct
    19921 gcttattctt aaataaccat ctggtttttt ccatataatt ctatctctat atctaaaacc
    19981 agcgttttgg aatattttaa tagcatctgc aacaataggg aatttttccc catctacgag
    20041 catatcatct atgtttaaaa cggcaattct accttctttt aatactctaa aaatttcttt
    20101 tgcaactcta ttaagaacac ctaaatattg ttcataattt ttgaataatc ccttataatc
    20161 aaaaggggca ttgaaataag gtggggaagt gaccattaag tgaatacttt catcttctat
    20221 ttcttccata ctcatacaat taccaattat aagtttatga tgagtcatta tatttttccc
    20281 gtttttaaaa ataagttaaa ttattttccc ctttttgtca atttcttgtt caatcatcag
    20341 ggatggttta taatgggaaa tttttacttt tataattttt tttgtccttg tcaataatta
    20401 ggatttcaac atcttttagt ttattagcaa tttcaattga ttttgttcca ataacaaaaa
    20461 atccagttgc taatgcatca gcagttatac aatcatctgc aatgattgtt atactaactg
    20521 gaaagtcctg aacagttttc ccgtttcttg ggtcaactat gtgagaaaat ttttgccctt
    20581 ttattttcac atattgttca taatcaccag aagttgctac acctttttca gttatttcta
    20641 tctttcctat tgttttttct ttttcaaagg ggtctcttat tgctattttc catttttttc
    20701 cagagggatt atttccccaa caatataaat ccccccctgc atttacaagt ccatttttaa
    20761 ttccttttga tttcagaaat tctactgctt tatcaacaat atatcctttt gcaattcctc
    20821 ctaaatcaat ttccatacca tcttttaaag agatattatt tcctttaatt tctatgtttt
    20881 tccagttgac ttttgtaagt gtatttttta tttcttcttc tccaggcggt tttcctgatt
    20941 tctcacattt tttatatagg tctacaagtg gcttacaact tatatcaaaa gcaccatctg
    21001 taattttaga aatttcaatt gcttttttta taacttctat cgtttcatta gatatagagg
    21061 cgcttttgtt tttatttaat ttactaactt cactttcttt ttcaaatata ctcaattttt
    21121 tttctaattc atctactttt ttaaatgcct gatttaaaat ttctttattg tcttttgttt
    21181 cttcaatagt tatttgaaaa aaagttccaa gtgcaattct tgtttcactt ttaaattcag
    21241 aatatcctgt ttggataaaa aggaaaaaca acaataggga aaaaaatttt ttcattgcag
    21301 gttgaacctt ttttacttga ttttttctaa aattctggag aaaagtgttt cttcatccgg
    21361 tatgtttttt atatttattt ctgtttttga aaaggcctct atgaagcatc ttataagtgt
    21421 attttttgtg attctttctc ttttaaattc ttttgttcta tttttatgta tttcccttac
    21481 aagtttatca agaaattcca aatgtgtatt ttttagcaga actgtaagac gaatatcaaa
    21541 tgtctgatat ttaggtccaa tttgcttttc aattatttct ttacttctct tcgtgggtaa
    21601 ttctttttct ggtatctttt tttcaactgt tggtttaatc cattctaacg ggtcttttcc
    21661 aagtcctgtt tttttcattt tttcttttcc tgtgcaataa tttcttttgt taattttcta
    21721 tagtcagatg caccatgtga atcaggagaa aactggtaaa ttgattttcc aaaacttggt
    21781 gcttctgcaa gtcggacatt ttctcttata aatgttttaa aaattttttc attgaaatga
    21841 ttctttactt tgtctgcaac atccttgcat atattttttc tcatatcaaa catagttaaa
    21901 ataattcctg atatttcaag agatgggttt agtcgttctt taacaagctg tattgtttgt
    21961 aataattttg taagtccttc caatgcaaaa aattctgcct gaatgggaat aaaaacttca
    22021 tttactgttg ttaaagcatt taaagttaaa agtccaagag aagggggaca atcaattaaa
    22081 atataatcaa atcttttttt gtattttttt aatatctctt tcaaaattat ttctctacca
    22141 acagcagtga cgagttctat ttcagcactt gcaagttcaa tttttgatgg caccaaataa
    22201 agataattat taatttgaac aattgtctca tcaatgggaa cttcatttat tagaacttca
    22261 tatattgatt tttctatatt aggtggttca aaacctaaat gtattgttgt atgtgcttgt
    22321 gggtcaaggt caattaaaag gacttttctt ttgttttctg atagacacca tccaatatta
    22381 actacacttg ttgtttttcc tgaaccacct ttttgattta taacagcaat ttttctcatt
    22441 acctctcctt aagttctgaa gttcataata taatattaca aattctgtat tttgacaaat
    22501 ttttagtatt gattacctca tattgcacaa aatcttcaat ttttatgtat tatactgcat
    22561 aaatttaata tgtgaaagta gcatttactg ggaaattgga attttgatag aggaaagtaa
    22621 atcataaaat tgtggaaatg atattttaac acaatcagta ttttcaattt tcgtttctcc
    22681 atctgcaata agtcctgcaa tggtcaaaca cattgcaatt ctatggtcat tccatgaatt
    22741 tacattatta ccttttaatt tttcaacacc atgaatttca aatccgtctt ctttttcata
    22801 tatttcacta cccatttttt taagttggct aacaattgct tttattctat ctgattcttt
    22861 atatcttaat tcttttgctc ctgaaataat tgtttttcca tttgaaactg atgcaattac
    22921 tccaattagt ggtatttcat caataataag aggcacttct ttttccccta tttcaattcc
    22981 atttaatttt cctttatatt ctatttcaat tgtcccaact tcttcattac atactttttt
    23041 ttggtcggtt atttttattt ctccacccat tttttcaatt acttttaaaa agtatgttct
    23101 tgtaggattt aaattaatat tttttattct tatttttgaa tttttgataa gcaaaccaat
    23161 tgcaataaaa taagaagcag aagaaaaatc acctggaatg ataacatttc ttccataaag
    23221 tttctggttt cctggaatat gaattttatt tccttctttt ataatttctc caccaaaata
    23281 ttgtagcatc ctctctgtat ggtctcttgt ctggtatggt tcttcaattg ttgtttctcc
    23341 atctgaaaaa agagaggcaa gtattataca acttttaacc tgagcagaag aaacaggaat
    23401 tttatagtca attccattta attttctacc ttttattaca ataggaggaa atgaattatt
    23461 ttctctacca attataagag ttcccatttt ttctaatggt tcaataattc ttttcatagg
    23521 tctttgtttt aatgaattgt cccctgtaag aattgatagg aaattttttg aagagagaac
    23581 tcctgaaagt aatcttattg ttgttcctga atttccacaa tcaagaatat cttctggttc
    23641 tctaaatgtg ttgccataaa ttttatatgt ttttcccttt tcaattttga tgcctgattt
    23701 ttcaaggcat tctaatgttg aaaaacaatc agaactttca agtaaatttt ttatttctgt
    23761 tgttccttct gaaatagcac caaaaattaa acttctatga gaaattgatt tatcaccagg
    23821 aacttctatt tctccttcta tattttcaac tcttttaact cttacatcca ttatattttt
    23881 tccctccaca ttgtttatat ttcaggtaca aaatggctaa atttaataaa gcaaatcccc
    23941 ctctttcccc gttgtgctat ctggaaatcc ccctttatcc ctcctccaaa atgttcgcca
    24001 cattcgccta tcattgcgaa tccgacgcgc tttctggcgg acgcgtcgga caggctttta
    24061 caaagtagga agtatctctc cttcccttaa taagggaagg atgggatgga ttttatttat
    24121 aaatccccct ctctccccct ttgctaaagg gggaagtatc aaaatccccc tgcccctgtt
    24181 gtgctatata agaaacgatg acgcaccaac tctgaggcac aaggtaagca actttagtcg
    24241 gtagcagttg tgctatctgg gagacgatga cgcaccaact ctgctttgct accctttgct
    24301 tctttagtcg gtttgttaaa gggggaagga tgggattttt ttcccgatta cttttattat
    24361 tggtgttatt tcatcaatta gtttgccaaa caggtcaaat gtaagtgatt gaaacccatc
    24421 tgataatgct tcttctggtt ttggatggac ttcaatcata agtccatctg cccctgctgc
    24481 tattgccgct ttactcattg gtattattat atctcttctt ccagttccat gacttgggtc
    24541 aactattact ggtagatgtg tttctttttt taaaacagga attgctgaaa ggtcaagggt
    24601 atttcttgta aaatcattat caaaactcct tatacctctt tcacataaaa ttacattttg
    24661 atttccttct ttaaggatat attctgcaca acttaaaaat tctttaattg ttgcagaaaa
    24721 tgaccttttt aaaagaattg gtttgtctat ttccccaact ttctttaata gaggataatt
    24781 atacatattt cttgccccta tctgtaaaat gtctgctact tctgctattt cttcaatttc
    24841 atcaattgat agaacttcgg ttattatagg tatttttact tcttctttta tatcttttaa
    24901 aatttcaagt ccttctttac caagtcctaa aaatgcataa ggagatgttc ttggtttgta
    24961 agcagcgccc cttaaaatat gtgccccttt acttttaata tgttttgcag tgcttagtag
    25021 ttgtgattta ctttcaatgg aacatggacc tgcaataatt gttatggtcc cttctcctat
    25081 ttttatgcta tttactttaa taattgtatt atggggatga aattctctac ttgcaagttt
    25141 gtagtccctt aatatactta taacttcaat aactcctggc ataattttaa aaacctcatc
    25201 tgatatgcct cttgtatcac ctataattcc aattattgtt ttttctgttc ccttacttat
    25261 gttaacatta aatcccatgt tttttatttt att
//
LOCUS       DAOOVW010000027        23723 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_1458_length_23723_cov_8.394710, whole genome shotgun sequence.
ACCESSION   DAOOVW010000027 DAOOVW010000000
VERSION     DAOOVW010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 23723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23723
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1458_length_23723_cov_8.394710"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            237..668
                     /locus_tag="PLW95_04990"
     CDS             237..668
                     /locus_tag="PLW95_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22021.1"
                     /translation="MKQKVGMVVLVIIILAALGFIVKQAMPKKPTYGINLVDVKAKKV
                     FQQTITAGETINYPVKSPYTGENTAYSANQCMKCGTIFAYIPYTPKSPTEEPPSPEIM
                     MPKCPKCGSFEIIVPAIPQGVKEIDVNQDEIPIVDMSKGTK"
     gene            complement(671..1333)
                     /gene="queC"
                     /locus_tag="PLW95_04995"
     CDS             complement(671..1333)
                     /gene="queC"
                     /locus_tag="PLW95_04995"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018624410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HOV22022.1"
                     /translation="MRKEKAVCLISGGMDSFVSAAIAKNNGYRIFCLTLNYGQKGKKE
                     IESAKKVGKFLKCEKHLILNFDLSWVKSALTRKEIKIPEKVKREIPLSYVPARNTILL
                     SVAVAYAETIDADAIFIGVNSVDYSNYPDCRPPFIKKFQEVVDIATKKTINGGKIKIQ
                     APLLYLSKADIVKKGIKLSLDFSITWSCYKSTKKPCGKCPSCQLRKNAFTKVGISDPL
                     TY"
     gene            complement(1378..2253)
                     /gene="xerC"
                     /locus_tag="PLW95_05000"
     CDS             complement(1378..2253)
                     /gene="xerC"
                     /locus_tag="PLW95_05000"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HOV22023.1"
                     /translation="MKRYVKKFLDYLTLQRNYSVNTIKGYKKDISQFINFIKKNKINS
                     FDEISHQLLISYLGHLRSYGYSENTIGRKIASLKSFFKFLTTRKLVKSNPIVLLSSPK
                     KADRLPNFLTLEEVEKILNLPFKNSWQSLRNKAILELLYSTGIRVGELTSLKIEDINF
                     FEEVIKVRGKGKKERIVPIGKYALKALIEYIEKRPNKKERIVFLNKYNKPLTERSVER
                     IIDKYSKKAIINKKVTPHTFRHSFATHLLDRGADLRTVQELLGHERITTTQIYTHLTV
                     ERLKEFYNKTHPRAK"
     gene            complement(2340..2455)
                     /gene="rrf"
                     /locus_tag="PLW95_05005"
     rRNA            complement(2340..2455)
                     /gene="rrf"
                     /locus_tag="PLW95_05005"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(2672..4369)
                     /gene="polX"
                     /locus_tag="PLW95_05010"
     CDS             complement(2672..4369)
                     /gene="polX"
                     /locus_tag="PLW95_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HOV22024.1"
                     /translation="MSKNTEIAEIFDKIADALEFKNDSSFRIGAYRKAARVIRELTED
                     IEDIWKRKELENIPGIGKGMAEKIDEYLSTGRMKKYEEAMEGISDGIIELLNIPNLGP
                     KTLNLVYNKLNVKNLSDLEKVIEDGSLAKLFGMGEKKVENIKRGIELYKLGKSRIPLG
                     IALPIVENIIESLRKYADKISPAGSIRRMKETIGDIDILAVGKEDIIEKFVSLPDVSE
                     ILVKGETKASVIVKENNLQVDIRVIPDDCYGSALQYFTGSKGHNIKLRTFAKENGLKI
                     SEYGVFKNDKKIAGKTEEDVYKALGLVWIPPELREDRGEIELAQQGKLPKIVEEKEIK
                     GDLHVHSEYSDGVNTIEEIALSVKKMGYEYIGIADHSKTSKIAGGLDEDELKRKNEEI
                     DKLNQKIKGIKILKGAEVDILSDGSLDYNDKILRELDYVIVAIHQGFKKNVGERIKKA
                     IENPYVDILAHPTGRLLSGREGYDVDIEEIIEYAEKYRVILEINSYPDRLDLNDINIL
                     KALGKNINFSIGTDAHNIGMLKYIKFGVGMARRGFLISKRIINTYPSEKIPFRRKAS"
     gene            complement(4362..4952)
                     /locus_tag="PLW95_05015"
     CDS             complement(4362..4952)
                     /locus_tag="PLW95_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010479675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOV22025.1"
                     /translation="MENIKLPEVEISEIGVEEALKRRKSTRDYKDEKLTIKEISQLLW
                     ACDGKNSKGGRTSPSAGATYPLEIFLIAGEIEKIEKGLYHYNFGEHSISLIKKGDLRN
                     EISKSAYEQRCLKIAPAIVVISGIFEKTTGRYGERGKRYVYMEVGHCGQNLHLQAEAL
                     SLGIVMVGAFDDEKVKGVLGIGEDVFYLCPVGKKNE"
     gene            complement(4994..5479)
                     /locus_tag="PLW95_05020"
     CDS             complement(4994..5479)
                     /locus_tag="PLW95_05020"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOV22026.1"
                     /translation="MSCKKFEKKLVDLINKELNKKEEEQILKHLEKCERCKNKYDELK
                     MVIEKSKKIDIPVLPSSWWDSRENSIFEVPVKEKRHIFKRRFVFALISIIFVFSLSLF
                     FFKSPKQSIVKNEENNKYSFYYDVLFLQDNLSLNENEILQMVDYLEEDEAEKILDSLL
                     K"
     gene            complement(5460..5987)
                     /locus_tag="PLW95_05025"
     CDS             complement(5460..5987)
                     /locus_tag="PLW95_05025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOV22027.1"
                     /translation="MGKKEKFEAFYREYRDRLYSVAFRMVGNKEDGIDIVQESFVKAY
                     QKWDSFREEAKFYTWIYRIVINLSYDYIRKRKREKIGEIQENILKSEKIESDKMVILR
                     DIKDEIQKEIENLTPRQKSIFILKTYEELPYKQIAEILKSREGTVKATYFQSVEKIRK
                     NLKEKGVLKNVMQEI"
     gene            6076..6366
                     /gene="rpsT"
                     /locus_tag="PLW95_05030"
     CDS             6076..6366
                     /gene="rpsT"
                     /locus_tag="PLW95_05030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOV22028.1"
                     /translation="MAKRTTSVLRRQRKEKRKTARNKAIKSKIKRIVKKTKKATLENN
                     VNLENYVKTAIKEIDKATGKGVLHKNTGSRKKSRLMHYINKYKTDTKNQEKK"
     gene            complement(6351..6995)
                     /locus_tag="PLW95_05035"
     CDS             complement(6351..6995)
                     /locus_tag="PLW95_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22029.1"
                     /translation="MKKENKKENLFVIVGVENTSKFEEIYKNLEEKYKFTDFDKKVFY
                     GGEVTTKDIIEEVDFLPVFSEKKLIIIKNVENLLKSECEKLEEIIEKAKDVIFILTGT
                     DINMPLKKYAEVYSAFKTQEQILFSQIYSIRKENKGKLRSLIREYVSQREKNFQVVIG
                     AANLYLKNVFLNRKKLDRETIKKFEQLHQLDFSLKIGKILPGEELEIFIYYFFS"
     gene            complement(6979..7476)
                     /locus_tag="PLW95_05040"
     CDS             complement(6979..7476)
                     /locus_tag="PLW95_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22030.1"
                     /translation="MDKKQKRKLALISLLLLLFSGCGYKFLNKGSEKVYIKKIENLTL
                     QPKLQMYLYNNLTDVIINYPSFTLTSSEDNADYVLTILLNKINRTPLFYDKYDSDEIV
                     SSKLTVEGEVLLERNGEVFKKSFVQFISYPLSNSYDEEKVLNDITKKIAIKVYTIILE
                     NEERK"
     gene            complement(7439..8365)
                     /gene="bamD"
                     /locus_tag="PLW95_05045"
     CDS             complement(7439..8365)
                     /gene="bamD"
                     /locus_tag="PLW95_05045"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOV22031.1"
                     /translation="MKRIFVIFVLFFAFAFSCFAYWEWTPQTGKWVNPKYAVKDTPKE
                     QFEYAEKFRQEGNIEKAIREHKKLLEHYKNSEYAPKSCFALGEIYYNQGEYKKAFDYY
                     QQIIDKYPQSELIFEAIKKQSEIGTKYLSIENKWQLPFLKSRKEEKGSLLEKVVDNSP
                     YSKEAPERLFNLGVFYMDVKNYERAEDIFRRIIETYPENPIIEKANFFLIKSEYLAIP
                     DVNYDIEKLEKVGNDISFFIEEYPDSTYNQELQKLADKIENEKAKRYYEIASLYERMG
                     KKGPSLFYYRKIVENYPETEYGQKAKKKISSH"
     assembly_gap    8500..8599
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8782..8854)
                     /locus_tag="PLW95_05050"
     tRNA            complement(8782..8854)
                     /locus_tag="PLW95_05050"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8819..8821),aa:His,seq:gtg)
     gene            complement(8915..10477)
                     /locus_tag="PLW95_05055"
     CDS             complement(8915..10477)
                     /locus_tag="PLW95_05055"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HOV22032.1"
                     /translation="MVDILIKNGLVFLNGKEFREVNVGVLGDKIIILNEKENIEAVKI
                     INAENKIVSPGFIDMHSHDDFALIFQSPYLPKTYQGVTTEITGNCGISGAPLGKGTEY
                     FLSTNSAVIGEIKDKNKWENWNEFIEQLERAPLPLNVVPFVGHGNLMSVFSEGKIHLE
                     EKNLKIVQQKLIEIMESGCFGLSTGLIYVPGMFSTTEELISLTKIISKFGGIYSSHIR
                     GEGKTLIEAVGEVNKIVEETGVSAEISHLKAAGIKNWNKIDKVIEIIEKQNNINFDVY
                     PYIASSTHISALLPPFIKKSNDIFSDLQNKEIREKVKKAMLEEDDWENYILSCGPEKI
                     FINSVISEKNKEIEGKNLKTISDIRNKDVFDCVIDIFVEEKGRAGIITFSMSEENVKK
                     LIKHKKGLIGTDGLPGKHPHPRLYGTFPRILNKYVRDEKLLSLEEALYKFTKGPAEKI
                     GLKRRGEIKDNYFADIVIFDPEKVKDKNTFESPEIQPEGILYVIVNGEIEVEDGKFTG
                     KTGGKVLKKNKF"
     gene            complement(10470..11435)
                     /locus_tag="PLW95_05060"
     CDS             complement(10470..11435)
                     /locus_tag="PLW95_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HOV22033.1"
                     /translation="MDIIKEIDIKKAYEVISDKIYLTPIISYAKINTQIGNNIFFKLE
                     NLQKTGAFKIRGVFNKINSLNKDEKARGVICASSGSHGIAVATVAKIYGIYSKVIVPE
                     ITPSIKIEKLKKLGEVEIYGKSYFESYVYAKKISEENNITFIHGFDDPLIIAGQGTVG
                     IEIIKQVENVDIIVAPIGGGGLIAGLLIARKSFFPSVKIIGVQAEGAPSMFISLNERK
                     LCELKEIKTIAEGIAVKKPGELNFSIVNKYIDDIVLVNDNEIIEGLCFLSKYTDIVPE
                     TAGVASFSAVLFNKLNIKNKKIVCIISGGNISKEDFLKYKEEENG"
     gene            11619..12974
                     /gene="mgtE"
                     /locus_tag="PLW95_05065"
     CDS             11619..12974
                     /gene="mgtE"
                     /locus_tag="PLW95_05065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOV22034.1"
                     /translation="MEKELLLPEIKELIKEKNFKELKEFMPELHPSDIADIIEDLEGD
                     DGAILFRILPKELAADVFSEFESTKQEEILKQLTDSQIQQLILELEPDDRISLFEELP
                     GEVTRGILNLLPHEERKQIAEILAYPENSVGRLITPEYITVKPNWTVEEALQHIKKFG
                     KDAETIDIIYVVDDNWKLLDDVPIRKFILANPDDKVDSLMDKKVISISAYKDQEEAIK
                     LVKQYNLVALPVTDSEGTLIGIVTVDDLIDVMEEEETEDFIKIGGLKTEGNLNVITDI
                     GEASIYKLYRSRISWLLILLFMDIITGSIIGVFQETLSKYVVLATFLPVLIDTSGNAG
                     SQAATLMVRAIALGDVKLNEWLKLISKELLVSTALGLTMGIGISFMGIIRGGSKVAFV
                     VSSSMIINVIIGSLIGISLPFLFTKFKKDPATASTPLITTIADIAGTFIYFLLATKFL
                     I"
     gene            complement(12975..13048)
                     /locus_tag="PLW95_05070"
     tRNA            complement(12975..13048)
                     /locus_tag="PLW95_05070"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13012..13014),aa:Arg,seq:tcg)
     gene            13207..13809
                     /gene="lexA"
                     /locus_tag="PLW95_05075"
     CDS             13207..13809
                     /gene="lexA"
                     /locus_tag="PLW95_05075"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOV22035.1"
                     /translation="MKKLTGRQKEILKFIKNFTEKNGYPPTIEEIMGKFSFSSPNAVI
                     SHLSALEKKGIIERKGNISRGIIIKETDKTYNIPVLGNIPAGIPLEEEENIEGYLTIS
                     ENLLSRDEYFALRVKGDSMKDAGIFDGDYVILKKDSEIKNGDIVVAYLDNQYTVKYFY
                     KKDSSIELRPANKFYTSIKVNKNQKFQIIGKVKGVFRKIL"
     gene            13806..14051
                     /locus_tag="PLW95_05080"
     CDS             13806..14051
                     /locus_tag="PLW95_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22036.1"
                     /translation="MSTEIKERIEVNAIFTKNKLKILWFKWKERKYFVKNITFSWKKH
                     SGDKEIYLFDLSNGIDVFEISFIPENLTWYLEKIYTY"
     gene            14053..15264
                     /gene="dinB"
                     /locus_tag="PLW95_05085"
     CDS             14053..15264
                     /gene="dinB"
                     /locus_tag="PLW95_05085"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOV22037.1"
                     /translation="MEKTIMHIDMDAYFASIEQVTNPFLKGKPIIVCGSYKTRSVVSS
                     CSYEAKKYGIKSGMSVKQALNLCPDVIIVSGYPEKYAEVSKEIFSIIKIFTENMEIYS
                     IDEVFLDISDIYHLYGGKKQLAFSIKKAIREKTSLPCSAGIGPNKLIAKIASCLCKPD
                     GIKVVEKEEVEKFICDLPVDKIPGIGEKTREKLEEFGIEKCKDIRKVGKEFLVEKFGI
                     IGEIIYKKSFGIGSQEIIKIHPPAKSIGHSYTLPFDTSNKEIINIILFKLSYKVAERM
                     RSENKHGKVITISLRFDDFSSITKRKKFVDIPADGKVIFKIGSFILEEIKIEKRIRAI
                     GITVGEIFDFNYDYLFKRENRREYLFHKIDDINNKFGEKTIFPAVLLLEKNLEVEKTH
                     SFVMWKFQKKD"
     gene            complement(15295..15711)
                     /locus_tag="PLW95_05090"
     CDS             complement(15295..15711)
                     /locus_tag="PLW95_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015774243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOV22038.1"
                     /translation="MKTHREEIWFNTKEKVEFINITERVQKAIDKSGIKEGIVLVNAM
                     HITASVFINDDELGLHNDFKKWLEKLAPHQPVSQYAHNVGEDNADAHMKRQIMGREVV
                     VAITDGKLDFGPWEQIFYGEFDGRRPKRVLIKIIGE"
     gene            15864..16547
                     /locus_tag="PLW95_05095"
     CDS             15864..16547
                     /locus_tag="PLW95_05095"
                     /inference="COORDINATES: protein motif:HMM:NF016269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HOV22039.1"
                     /translation="MKKITVFVICLLFFISSNLFALSKESLVERIKMAEKLLDDFTTA
                     PDRQIPQELINQAEGIIFMKQYKGGFVLGAKGGNGIILAKDRNTGKWSAPAFVSNVEG
                     SFGFQAGGQSIETIVLIMNKEGLDMLLKSRVKIGVDISAAAGPYGRDAAAQLGAGVGL
                     LIYSRAKGLYAGASIEGGFLAIDEEANQIFYGKDVKVRDILIRRTVKIPEEAKTLIQK
                     LENYAQITE"
     gene            complement(16563..17390)
                     /locus_tag="PLW95_05100"
     CDS             complement(16563..17390)
                     /locus_tag="PLW95_05100"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOV22040.1"
                     /translation="MKKKLLLLLFLPFIFAGCQSIATKEDVGTVKEKVTSVEQDLYLT
                     SKAIADKFKEIENDYNNKIDNLNVKVESFKKDQAIILERISTIEKEVNLIKGKIDEVN
                     YNFEKKVEQQNDNIEKNTIEVRRDIDSLKKTYTDIISSIATLNSSLTLMQKDLSVLKK
                     SQEEIISDNTLKYSELEELLNKNSKIFLDELTKHESEIYFLKNKISQSGTTQISEKET
                     SKEVSKELSSDVKYYIVKKGDNLSEIARKHNTTISAIKKLNNLKNDTVYVGQKLKIP"
     gene            complement(17387..18001)
                     /gene="pal"
                     /locus_tag="PLW95_05105"
     CDS             complement(17387..18001)
                     /gene="pal"
                     /locus_tag="PLW95_05105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HOV22041.1"
                     /translation="MKYLKFGLVFLVAVLFASCQCPGRKKEAKKEEVKKEEVKEATPI
                     SKVIEEIEKPVEKPKVEEKKEEVKVAVGEEVPPQKLSEGAKVEKPEGEVAAIFENIYF
                     NFDKYDLKQDSIEKLKKIGDYLKANPEVKILIEGHCDERGTREYNLVLGEQRALSARN
                     FLINYGISPSRLYTVSYGEDNPADPRHCEEAWAKNRRCEFKIVK"
     gene            complement(18080..19297)
                     /locus_tag="PLW95_05110"
     CDS             complement(18080..19297)
                     /locus_tag="PLW95_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22042.1"
                     /translation="MRKFLLFFFLIIMPCFSVPQVFLQITKDFTQKAEVVLLFQIEKN
                     NYWENFSNTLSKDLFYSGYFNIKSENFVEDIEESKQDFPVNIIISVKNEKGNIVVSVF
                     DNLEEQELFRKNYNILKDPFYFAHVVNDDIVLFLTGKPGIAQSRVIFVANKTGKYQIY
                     VCDYDGRNLKQITNFNFLVNFPVWTGDRSGFYFVSYKNNYPEIAKYDFNTGKEETIFQ
                     YPGLNACVAPCKQKNILGVVLSKSGNPEIYLATIDGKIIRRLTNYKGVDSSPSFSPDG
                     EKIAFVSDKYGTPQIFIMDSDGFGVKRISYEFSYSTSPKWSPDGNYIAYIVRTGDYKI
                     AIFDVKTNKTKILSNSSGSETISWASDSRHIVFSKVNIYPSKICIIDVFTGETRDLTN
                     FKYDTFSPDWEKF"
     gene            complement(19297..20103)
                     /locus_tag="PLW95_05115"
     CDS             complement(19297..20103)
                     /locus_tag="PLW95_05115"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOV22043.1"
                     /translation="MRNRESRFGDTGKVKKRSFLLSVVIHIVAGVFLIFSSVNMKRKE
                     NVFFVQIVDIPGVMQVINTPVGENYKTEEEKEVKKEVKEKYQEKTKISSEKKGSSGRK
                     SSSAGEISPSSKTSSFNADEYKKELLSKIGNREISSSNTKGSSNKTNIKQEISQEDMS
                     TLFSTSLNLENLRISNDIPSWYISLLKNKIEENWNFGNIIGDFSTIISFRIYKNGEIS
                     NLRIEKSSGYKPFDKSAESAIKSTKNIPPFPEEIKEEYLEVTIEFKKEEG"
     gene            complement(20061..20459)
                     /locus_tag="PLW95_05120"
     CDS             complement(20061..20459)
                     /locus_tag="PLW95_05120"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOV22044.1"
                     /translation="MNNYFDNKNSSILSNISITNLVDVALNLVIMLLMMAPLIEQGIE
                     VKLPESSPSKVEVEKSVVITVAKDETYYLGSTKITLRDLYKELKEKKGTGISVIVKGD
                     KDVSYNNIIKVLDIIKECEIEKVGLATQVK"
     gene            complement(20452..21054)
                     /locus_tag="PLW95_05125"
     CDS             complement(20452..21054)
                     /locus_tag="PLW95_05125"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOV22045.1"
                     /translation="MGNIWVSSWQLSDWAGKLDIVILLFLSIYSWYIMFYKFFYLRSV
                     ERKNNIMKDIITYGKKIIVIKCPLFFVLKECKRIIDKKGDIKEEEIEKIIFIESNKLK
                     TDLSFLATITSIAPFLGLLGTVWGLLMAFNNIAATGSASTRVISSGVAEALITTVYGL
                     LVAIPAAVGYNYFQDKTEKIKTDMFSITGDILNYLKIKNE"
     gene            complement(21078..21536)
                     /locus_tag="PLW95_05130"
     CDS             complement(21078..21536)
                     /locus_tag="PLW95_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22046.1"
                     /translation="MKKIFFIFVIIIGLLYGEEKFDGLKEPVRSNVYKTFSVDVNKRT
                     LGAYNLSEIKNSRSVSFLILLLDDEEFTWCKYDGKGVWTSPAKEAIEAIIKIGKPSLE
                     YIFQVLEDKHPYIELNKTIEKNLIYVLKKITNKNFEAREEWINWWNKEKS"
     gene            21623..22423
                     /locus_tag="PLW95_05135"
     CDS             21623..22423
                     /locus_tag="PLW95_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22047.1"
                     /translation="MGKKLIILIFYFAFIPSIFCLSKEDYNVILKRNIFTTPYIEEKE
                     IKKESILKPPPPPSLNTLIKAKGVILIGETGYGIVEVKGKDIIVRKNEKILGAEVVKI
                     ENDSIVFFYNDKEEKLQIEKITEKGNFVKATPEINVKIEENINTNKEEKEISQPEFKE
                     PVVVDFEKTISELKNDKDLFNNVNIIPNIEEGNVNGFKINNLPENSIPFQYGLRNGDI
                     IYRVNGIVINSMPKGLAVYNQIVKSGTKTVTVEVIRNGSPVVFSYTLK"
     gene            22512..23504
                     /gene="amrS"
                     /locus_tag="PLW95_05140"
     CDS             22512..23504
                     /gene="amrS"
                     /locus_tag="PLW95_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOV22048.1"
                     /translation="MKEAIFWKKENDKIRCALCNHFCLIGEGKTGLCGVRLNKTGVLY
                     SLVYGKTVAEHIDPIEKKPFFHFLPGSFSYSIATVGCNFFCPYCQNYEISHPQEIFYT
                     NQTTPEEIVNKSLSSNCKSISYTYTEPTVFFELAFDTSKIAKQKGIKNNFVTNGYMSK
                     EALKTISEFLDAANVDIKGDEDFYKKLCKTKQKYVIDNISLMKELGIWVEITTLIIPE
                     YNDSEKQLKDIAKIIKDIDIAIPWHVTRFYPTYKMSDHYPASIEKIKRAREIGFETGL
                     KYVYTGNIYIEKGENTYCPSCKKLLIERSGFSILKNNIKNSKCCFCSERIDGVF"
     gene            23550..23622
                     /locus_tag="PLW95_05145"
     tRNA            23550..23622
                     /locus_tag="PLW95_05145"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23583..23585,aa:Val,seq:tac)
BASE COUNT         8129 a         3805 c         2970 g         8719 t
ORIGIN      
        1 aatctattcc ctctttcatt ttttaaaatt aaggaaaaag aggtaaaata tataataaag
       61 tggaaaaaat ttaaatggaa atttttattg atagaaaaaa attattagat ataattttaa
      121 acatagtata aaattagttg tataaaagaa ctctcacatt aaatatttta tcctataaag
      181 aagtaagcgt gagagaaaaa atatagataa ttttctaaaa taaaaaagga gggaatatga
      241 aacaaaaagt aggtatggta gttttagtaa ttataattct tgctgcgttg ggttttattg
      301 ttaaacaagc gatgcctaaa aaaccaactt atggaataaa tctggttgat gttaaagcaa
      361 aaaaagtttt tcaacaaaca attacagctg gtgaaacaat aaattatccc gtaaaatcac
      421 cttatactgg agaaaatact gcttattctg ctaaccaatg tatgaaatgt ggaacaattt
      481 ttgcttatat tccttatact ccaaaaagtc ccacggaaga accaccatca cctgaaatta
      541 tgatgccaaa atgtccaaaa tgtggttcat ttgagataat agttcctgca attccacaag
      601 gagtaaaaga aattgatgta aatcaggatg aaatacctat tgttgatatg tcaaaaggta
      661 caaaataact ttagtaagtt aaagggtcag aaattcctac tttggtaaag gcattttttc
      721 ttagttggca acttggacat tttccacatg gttttttagt acttttataa caggaccagg
      781 taattgaaaa atcaagtgag agttttatac ccttttttac tatatctgct tttgataaat
      841 aaagtaatgg tgcttgtatt ttaatttttc cgccatttat tgtttttttt gttgcaatat
      901 caacaacttc ctgaaatttt tttataaaag gtggtcggca atctggataa ttagaataat
      961 caacagaatt tactccaata aaaattgcat ctgcatcaat tgtctctgca taggcaacag
     1021 caacagaaag taaaattgta tttcttgctg gaacataaga taaaggtatt tctcttttta
     1081 ccttttctgg tatttttatt tcttttcttg taagtgctga ttttacccac gagaggtcaa
     1141 aatttaaaat taagtgtttt tcacatttta aaaatttacc cactttcttt gccgattcaa
     1201 tttctttttt acctttttgt ccataattta aggttaaaca aaaaatccta tatccattat
     1261 ttttagcaat agcagcagag acaaaactat ccattccacc ggaaattaaa caaactgctt
     1321 tttcttttct catagtaaaa ttatataaaa aataataaaa attgttaata aggtaggtta
     1381 tttagcgcgt ggatgtgttt tgttataaaa ttcttttaat ctttctactg ttaagtgagt
     1441 ataaatctga gtggttgtta ttctttcatg tccaagaagt tcttgaactg ttcttaaatc
     1501 agcacctcta tcaagtaggt gagttgcaaa agaatgtctg aaagtatgtg gtgttacttt
     1561 tttatttata attgcctttt ttgaatactt atcaattatc ctttcaacac ttctttctgt
     1621 cagaggttta ttgtatttat tgagaaaaac aattctttcc tttttattcg gccttttttc
     1681 aatatattct atcaatgcct ttaaagcata tttcccaatt ggcacaattc tttctttttt
     1741 accttttccc ctgaccttta taacttcttc aaaaaaattt atatcctcaa tttttaaaga
     1801 agtaagttcc ccaactctta ttcctgttga atataaaagt tcaagtattg ctttatttct
     1861 taatgattgc caactatttt taaatggtaa atttaaaatt ttttcaactt cttcaagtgt
     1921 taaaaagttt ggcaacctat ctgctttttt tggggaagaa agaagaacaa tcggatttga
     1981 ttttaccaat tttcttgtag ttagaaattt aaaaaatgat tttaatgatg caatttttct
     2041 accaattgtg ttttcagaat aaccataact tcttaaatgc cctaaataag aaatgaggag
     2101 ttggtgagaa atttcatcaa aagaatttat tttatttttt tttataaaat ttataaattg
     2161 agaaatatct tttttatatc ctttaattgt attgactgaa taatttcttt gtaatgtcag
     2221 ataatcaaga aattttttaa catacctttt cattcttctt tatttctttt tcaatgtact
     2281 tttatgcctt ttagacacct gcctgaatat ctaccacttt tcatcactaa aaaaaatgtc
     2341 tctggcaata cctactctcc cacctcctct cgaaggcagt accatcggcg tcgagaggct
     2401 taactgccgt gttcgggatg ggaacgggtg ttaccctctc gctatcgttg ccagagacat
     2461 tattatttta ccttcttttt cttatctgtc aacatttcga attgagacat aataaatatt
     2521 tacaaaaaaa agagaaaata aaatgactta ctttcctgct cttttccttt ccataccaaa
     2581 atactcatca gataaacatc ggaaagaaag taataagtaa ttttcattac taataaaaaa
     2641 aatacgagag aattaatatt atatgagaat tttaggatgc ttttcttcta aatgggattt
     2701 tttcagatgg ataagtattg ataattcttt ttgatattaa aaaacctctt cttgccatac
     2761 caacaccaaa tttaatatat tttagcattc ctatattatg agcatctgtt ccaattgaaa
     2821 aatttatatt tttaccaagt gccttcaaaa tattaatatc atttaggtca agtcggtcag
     2881 gataagaatt tatttcaaga attactctat atttttctgc gtactcaata atttcctcaa
     2941 tatcaacatc atatccctct cttccgctta ataatcttcc tgttggatga gccaatatat
     3001 caacataagg attttctatt gcttttttaa ttctttctcc gacatttttc ttaaaaccct
     3061 gatggattgc aacaattaca taatcaagtt ctcttaagat tttatcatta taatcaaggg
     3121 aaccatcact aagaatatca acttctgcac cttttaatat ttttattccc tttatttttt
     3181 gatttaattt gtctatttct tcattttttc tctttaattc atcttcatca agacctcccg
     3241 ctatttttga tgtctttgaa tggtcagcaa ttccaatata ttcatatccc atttttttta
     3301 cagataaagc aatttcttca attgtattaa caccatctga atattcagaa tgtacatgta
     3361 aatcaccttt tatctctttt tcttcaacaa tttttggcaa tttaccttgt tgcgctaatt
     3421 caatttcacc tctatcttct ctcaattcag gaggaatcca gacaagtccc aatgctttat
     3481 aaacatcttc ttctgtttta cctgctattt tcttatcatt tttaaaaaca ccatattcac
     3541 ttatttttaa tccattttct tttgcaaaag ttcttaattt tatgttatga cctttggaac
     3601 cagtaaaata ttgtaaggca gaaccataac aatcatcagg aataactctt atatcaacct
     3661 gcaaattatt ttcttttaca atcacagatg ctttagtttc tccttttaca agtatttcac
     3721 ttacatctgg taaagataca aatttctcaa ttatatcttc tttccctact gcaagaatat
     3781 caatatcacc aattgtttct ttcatccttc taattgaacc agcaggagaa attttatcag
     3841 catattttct taaactttca attatgtttt caactattgg aagtgcaatt cccaatggta
     3901 ttcttgattt acctaacttg taaagttcta tacctctttt tatattctca actttcttct
     3961 ctcccattcc aaaaagtttc gcaagtgaac catcttcaat aactttttca aggtcagata
     4021 aatttttaac atttaatttg ttatagacca aattcaatgt ttttggtcct aaattcggga
     4081 tattgagtaa ttcaataatt ccatcagaaa taccttccat cgcttcctca tattttttca
     4141 tcctgcctgt acttagatac tcatcaattt tttctgccat tccttttcca atcccaggga
     4201 tattttcaag ttccttcctt ttccatatat cttcaatatc ctcagtaagc tctcttataa
     4261 cccttgctgc tttcctataa gcacctattc taaaagaact atcatttttg aattcaaggg
     4321 catcagcaat tttatcaaaa atttcagcaa tttcagtatt tttactcatt ttttttacct
     4381 actgggcata aataaaaaac atcttcacca atcccaagaa caccttttac tttttcatca
     4441 tcaaatgcgc caaccataac tattccaaga gataatgcct ctgcctgtaa atgtaaattc
     4501 tgcccacaat gtccaacttc catataaaca tatcttttac ctctttcccc atatcttcct
     4561 gttgtttttt caaaaattcc agatataaca actattgcag gagcaatttt taagcatctc
     4621 tgctcataag cactttttga aatttcattc cttaaatcac ctttcttaat taaggaaatt
     4681 gaatgttccc caaaattgta atgatataaa cctttttcaa ttttttctat ttcacctgca
     4741 attagaaata tttcaagagg atatgttgcc ccagcagaag gagatgttct tccaccttta
     4801 gaatttttgc catcacatgc ccataaaagt tgagaaattt cttttattgt caatttttca
     4861 tctttataat ctcttgttga cttccttctt tttaatgcct cttctacacc tatttcacta
     4921 atttcaactt ccggtaattt tatattttcc attttttatt attttacctt aaaaaagagg
     4981 gaggagggaa aagctattta agcaatgagt ccaaaatttt ttctgcttcg tcttcttcta
     5041 aataatcaac catttgaaga atttcatttt catttaagga taaattatcc tgtaaaaata
     5101 aaacatcata ataaaaacta tatttattgt tctcttcatt tttcacaata ctctgttttg
     5161 gggatttaaa gaaaaaaaga gaaagagaaa aaacaaaaat aattgaaatc aatgcaaata
     5221 caaatctcct tttaaatata tgcctctttt ctttaacagg tacttcaaaa atagaatttt
     5281 ccctgctatc ccaccaagaa gaaggaagaa caggaatatc tatttttttg cttttttcaa
     5341 ttaccatttt caactcatca tatttatttt tacatctttc acatttttct aaatgcttta
     5401 aaatctgttc ttcctctttt ttattaagtt ccttattaat taagtcaacc aatttctttt
     5461 caaatttctt gcatgacatt tttcagcacc cccttttctt taagattttt ccttatcttt
     5521 tcaactgatt ggaaataagt tgcttttact gttccttctc ttgatttaag tatctcagca
     5581 atctgtttat atggcaattc ttcatatgtt ttcaaaataa aaattgattt ttgtcttggc
     5641 gttaaatttt caatttcttt ctgaatttca tctttaatat ctcttaaaat aaccatttta
     5701 tcactttcaa ttttttcact ttttaaaata ttttcctgaa tttctcctat tttttccctt
     5761 tttctttttc taatgtagtc atatgaaaga tttatgacta ttctataaat ccatgtataa
     5821 aacttcgctt cctctctaaa actatcccat ttctgatatg ccttaacgaa actttcctga
     5881 acgatatcaa taccatcttc tttatttccc accattctga aagcaacact atacaatcta
     5941 tctctatatt ctctataaaa tgcctcaaat ttttctttct tacccacttc tttttgacct
     6001 ttttgttgat aaatagttta gtaaatagta taataaattg aacaaaataa aaaggataaa
     6061 aaaacaggag gaaaaatggc taaaagaacc acatctgttt taagaagaca aagaaaagaa
     6121 aagagaaaaa cagcaaggaa taaggcaata aaatcaaaaa taaaaagaat agttaagaaa
     6181 acaaaaaaag caactcttga aaataatgtg aatcttgaaa attatgtgaa aacagcaata
     6241 aaagaaatag ataaagcaac aggtaaagga gttcttcata aaaatactgg ctcaaggaaa
     6301 aaaagtagac ttatgcatta cataaataag tataaaacag acaccaaaaa tcaggaaaag
     6361 aaataataaa taaaaatttc aagttcctct cctgggagaa ttttccctat ttttaaagaa
     6421 aaatctaatt gatgcagttg ctcaaatttc ttaattgttt ctctatctaa ttttttcctg
     6481 tttagaaata cattttttaa atataaatta gcagcaccta taacaacttg aaaattcttt
     6541 tctctttgtg aaacatattc ccttattaaa cttcttaatt ttcccttatt ttcttttctt
     6601 attgaataaa tttgagaaaa aagtatctgt tcttgtgttt taaatgcaga ataaacttct
     6661 gcatattttt tcaaaggcat atttatatcc gttcctgtta aaataaaaat aacatctttt
     6721 gccttttcaa taatttcttc taatttttca cattcacttt tcagaagatt ttcaacattc
     6781 ttaataataa taagtttttt ttcagaaaaa acagggagga aatcgacttc ttcaattata
     6841 tctttggtag ttacttctcc accataaaaa acttttttat caaaatcagt gaatttatat
     6901 ttttcttcta aattcttata aatttcttca aattttgatg tgttttcaac tccaacaata
     6961 acaaacaaat tttctttttt attttctttc ttcattttct aaaataattg tgtaaacttt
     7021 tattgctatt tttttagtaa tatcattaag aactttttct tcatcatagg aattggaaag
     7081 aggatatgag atgaactgaa caaaactttt tttgaaaacc tcaccatttc tttcaagcaa
     7141 aacttcacct tccactgtca atttactact tacaatttca tcactatcat atttgtcata
     7201 aaaaagagga gttctattta ttttattaag cagaattgtc aaaacataat cagcattatc
     7261 ttctgatgaa gtaagtgtaa aagatggata attaattatt acatctgtca ggttgttata
     7321 tagatacatt tggagttttg gctgtaatgt caaattttca attttcttta tataaacctt
     7381 ttcacttccc ttatttaaaa acttataacc acatccagaa aataaaagaa gtaataaact
     7441 aatgagagct aattttcttt tttgcttttt gtccatattc tgtttcagga taattctcta
     7501 caatttttct ataataaaat aaagaaggtc cctttttccc cattctttcg taaagggagg
     7561 caatttcata ataccttttt gctttttcat tttctatttt atctgccaat ttttgaagct
     7621 cctggttata agtactatca ggatattctt ctataaaaaa agatatatca tttcccactt
     7681 tttcaagttt ctcaatatca tagttcacat caggaattgc aagatactct gattttatga
     7741 gaaagaaatt tgctttttct attattggat tttcagggta tgtttctata attcttctaa
     7801 atatatcttc tgccctttca tagtttttca catccatata aaacacacct aaattgaata
     7861 acctttcagg tgcttcttta ctataagggc tattatccac aactttttca agtagggatc
     7921 ctttctcttc tttgcgggat tttaaaaatg gtaattgcca tttattttca atggaaagat
     7981 actttgtccc tatttctgat tgttttttta ttgcttcaaa tataagttct gactgtggat
     8041 atttatcaat aatttgttga taatagtcaa atgccttttt atattcaccc tgattataat
     8101 aaatttcacc aagagcaaag catgatttag gagcatattc tgaattttta taatgctcta
     8161 acaacttttt atgttccctt atcgcttttt caatattccc ttcttgcctg aatttttctg
     8221 catattcaaa ttgctctttt ggggtgtcct ttactgcata ttttggattt acccactttc
     8281 ctgtttgagg tgtccattcc caataagcaa aacaagaaaa agcaaaagca aaaaataaaa
     8341 caaaaataac aaaaattctt ttcatttatc tcctatatta taattttact tttatcttta
     8401 taatattttc cacaaaataa tagatgtgtc aaataagcaa gaaaattgct aaacagggtg
     8461 agtgagggga ttgttctaca caggaagttt agagggatgn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnt tctgcatccc ctccctttca gagaagattg ctaaacaggg
     8641 tgagtgaggg gattgttcta cacaggaagt ttagagggat gcctacgaac caactctgct
     8701 tcgctactct ttgctacctc agtcggtttg acccccatgg ggttctgcat cccctccctt
     8761 tcagagaaga ttgctaaaca gggtgagtga ggggatttga acccccgacc cctggagcca
     8821 cagtccagtg ctcttgccac tgagctacac tcaccattac tttatatttt atcttttctt
     8881 ctcttctatg taaatttacc aaattaatta cacatcaaaa tttatttttt ttaagaactt
     8941 taccacctgt tttgcctgta aattttccat cttctacttc tatttcacca tttactatta
     9001 catataaaat accttctggt tgaatttcag gactttcaaa tgtgttttta tcttttactt
     9061 tttcagggtc aaaaataact atatcagcaa aatagttatc ttttatttct ccccttcttt
     9121 tcagtcctat tttttctgct ggaccttttg taaatttata aagtgcctct tctaaactca
     9181 acaatttttc atctcttaca tatttgttta aaattcttgg aaatgttcca tataaacgag
     9241 gatgtggatg ctttcctggt agtccatcag ttccaataag tccctttttg tgttttataa
     9301 gtttctttac attttcttca ctcattgaaa aagtaataat tcctgctctc cctttttctt
     9361 caacaaaaat atcaattaca caatcaaaaa catccttgtt tctaatatct gaaattgttt
     9421 ttaaattttt cccttcaatt tctttatttt tttctgaaat tacactattt ataaatattt
     9481 tttcaggtcc acaacttaaa atataatttt cccaatcatc ttcttctaac atcgcttttt
     9541 tgactttttc ccttatttcc ttattctgta aatcactaaa aatatcatta ctttttttta
     9601 taaatggcgg aagtaaagca gaaatatgtg ttgaagaagc aatataagga tatacatcaa
     9661 aatttatatt attctgtttt tcaataattt caattacttt atcaatttta ttccagttct
     9721 ttatccctgc tgctttaaga tgagagattt cagcagagac acctgtttct tctactattt
     9781 tattaacttc gccaactgct tctatcaatg tcttaccttc acctcttata tgtgaagaat
     9841 aaataccacc aaattttgaa attattttag taagagagat taactcctct gttgtgctga
     9901 acataccagg aacataaata agacctgtgg acaaaccaaa gcatccactt tccataattt
     9961 caataagttt ttgttgaaca atttttaaat ttttctcttc cagatgtatt ttcccttctg
    10021 aaaaaacaga cattaaatta ccatgcccta caaaaggaac aacatttaaa ggaagaggcg
    10081 ccctttctaa ttgttctata aattcattcc aattttccca tttattttta tctttaattt
    10141 ctcctattac tgctgaattt gtacttaaaa aatactctgt tccttttcca agtggcgccc
    10201 ctgaaattcc acaattccca gttatttctg ttgtaactcc ctgatatgtt ttaggtagat
    10261 atggactttg aaaaattaaa gcaaaatcat catgactatg catatcaata aaaccaggtg
    10321 aaacaatttt attctctgca ttaattatct taactgcctc tatattttct ttttcattta
    10381 aaattattat tttatcaccc aggacaccaa cattaacttc tctaaactct ttgccattta
    10441 aaaacacaag accatttttt ataagtatat caaccatttt cttcctcctt atattttaaa
    10501 aaatcctctt ttgaaatgtt cccaccactt attatacaaa caattttttt atttttaata
    10561 tttagtttgt taaaaagaac cgctgaaaaa gatgcaactc ctgctgtttc aggaacaata
    10621 tctgtgtatt ttgaaaggaa acacaagccc tcaattattt cattatcatt tacaagaact
    10681 atatcatcaa tatatttatt tacaattgaa aaatttaact cacctggttt tttaacagca
    10741 attccttctg caattgtttt tatttcttta agttcacata attttctctc gtttaaagaa
    10801 atgaacatag aaggagcacc ttctgcttga actccaataa tttttacaga agggaaaaag
    10861 gactttcttg caataagaag ccctgcaatt aaaccacctc caccaatcgg agcaacaata
    10921 atatctacat tctctacctg ttttattatt tcaattccaa ctgtcccctg tcctgcaata
    10981 ataagagggt cgtcaaaacc atgaataaaa gttatattat tttcttcaga tatttttttt
    11041 gcatatacat aactttcaaa ataacttttc ccatatattt ccacttcacc caattttttc
    11101 aatttttcaa ttttaataga gggggtaatt tcaggcacta taacttttga gtatattcca
    11161 taaatttttg caacagtcgc aactgcaatt ccatgactgc cagaagaagc acatataact
    11221 cctcttgctt tttcatcttt attgagagaa ttgattttat tgaaaactcc tcttatttta
    11281 aatgcccctg ttttttgtaa attttcaagt ttgaaaaata tattattccc tatttgggtg
    11341 ttgatttttg cataagaaat aataggagtt aaataaattt tatcagatat aacttcatat
    11401 gcttttttta tatcaatttc ttttattata tccattcttg ttttaattta attcttcttc
    11461 taaatcagtt tttcataaat tttatcatcc tatttttact tattatttcc attgtgtcaa
    11521 cttcatcaaa aattttgaca aagttaatac attaaaataa acttatttac agcatcaaaa
    11581 aagaaaattt acatacttta tttttttaaa aaagaaaaat ggaaaaagaa ttattattac
    11641 ctgaaataaa agaacttata aaagagaaaa atttcaaaga attgaaagaa tttatgcctg
    11701 aattacatcc ttctgatata gcagacataa ttgaagacct tgaaggagat gatggtgcta
    11761 tattatttcg tattttacca aaagaattgg cagcagatgt gttttctgaa tttgaatcaa
    11821 caaaacaaga agagatatta aaacaactca cggatagtca gatacaacaa cttattcttg
    11881 aacttgaacc agacgataga atttctttat ttgaagaatt accaggagaa gttacaaggg
    11941 gtattcttaa tcttttaccc catgaagaaa gaaaacaaat tgctgaaata cttgcatatc
    12001 cagaaaatag tgttggaaga ttaataactc ctgaatatat cacagttaaa ccaaactgga
    12061 cagttgaaga ggcacttcaa catataaaaa aatttggtaa agatgctgaa acaattgata
    12121 ttatttatgt tgttgatgat aactggaaat tacttgatga tgttcctata aggaaattca
    12181 tacttgcaaa tccagatgat aaagttgatt ctttgatgga taaaaaagtc atttcaattt
    12241 ctgcttataa ggaccaggaa gaggcaataa aacttgtaaa acaatataat ttagtagcac
    12301 ttccagtaac tgactcagaa ggaacactta taggaattgt aactgttgat gaccttattg
    12361 atgttatgga agaagaagaa acagaggact ttataaaaat aggtggattg aaaacagagg
    12421 gaaatttaaa tgtaataaca gatatcggag aagcatctat ttataaactt tatagaagta
    12481 gaataagttg gcttttaatt cttcttttta tggatataat tactggaagt ataatcggcg
    12541 tctttcaaga aacactctct aaatatgtag tgcttgcaac tttccttcct gttttaattg
    12601 atacaagtgg taatgctggc tctcaggcag caactttaat ggtaagagca attgctcttg
    12661 gcgatgttaa actaaacgag tggctaaaac ttataagtaa agaactcctt gtttcaactg
    12721 ctcttggttt aacaatggga ataggtattt cttttatggg aataataaga ggaggaagta
    12781 aagttgcttt tgttgtatca tcctcaatga ttataaatgt cataatagga agtttaattg
    12841 gtatttctct ccctttctta ttcacaaaat tcaaaaaaga ccctgcaact gcaagcactc
    12901 ctttaataac aacaatcgct gatatagctg gaacttttat ttacttctta ctcgccacaa
    12961 aattccttat ataatgcgcc tggcgcgact tgaacgcgca accattggat tcgaagtcca
    13021 ccgctctatc cagttgagct acaggcgctt ccttaaatta taccttgaat ttcttataaa
    13081 aacatctgtg tttccagaat attaatttac aaaaataaat cgttcttctc ttaattaccg
    13141 aaaataaaaa gatatttaca cttgtaaaaa tttatagtat aattatttac aggtaaggag
    13201 aaaaaaatga aaaaattaac tggaagacag aaagaaatat taaaatttat aaaaaatttt
    13261 acagagaaaa atgggtatcc accgacaatt gaggaaataa tgggaaaatt ttctttttca
    13321 agtccaaatg cagttatttc tcatttatct gctcttgaaa aaaagggaat tatagaaaga
    13381 aaaggaaata tatcaagagg aattataatt aaagagacgg ataaaacata taatatacct
    13441 gttttaggaa atatacctgc tggaattcct ttagaggagg aagaaaatat tgagggatat
    13501 ttaactattt ctgaaaatct cctttcaaga gatgaatatt ttgctttaag ggtaaaagga
    13561 gatagtatga aagatgcagg tatttttgat ggtgattatg taattttaaa aaaagatagt
    13621 gaaattaaaa atggagatat tgttgttgct tatttagata atcaatatac agtgaaatat
    13681 ttttataaaa aggatagttc tattgaattg agacctgcaa ataaatttta tacctcaata
    13741 aaagtaaata aaaaccaaaa atttcagata attggaaaag ttaagggagt tttcaggaaa
    13801 attttatgag cacagaaata aaagaaagaa tagaagtaaa tgcaatattt acaaaaaata
    13861 aattaaagat attgtggttc aaatggaaag aaaggaaata ttttgtcaaa aacataactt
    13921 tttcatggaa aaaacattca ggagataagg aaatttattt gtttgattta agtaatggaa
    13981 tagatgtgtt tgagatatct tttattccag aaaatcttac atggtatctt gaaaaaatat
    14041 acacctatta aaatggaaaa aacaattatg catattgata tggacgcata ttttgcttct
    14101 attgaacagg ttacaaaccc ttttttaaaa ggaaaaccaa ttattgtttg tggttcatat
    14161 aaaacaagga gtgtggtatc ttcctgttcc tatgaagcaa aaaaatatgg aattaaatct
    14221 ggtatgagtg taaaacaggc attaaattta tgtccagatg ttattattgt aagtggatat
    14281 cctgaaaaat atgcagaagt atcaaaagaa attttctcaa ttataaaaat ttttactgaa
    14341 aatatggaaa tatattccat agacgaagtt ttccttgata tttcagatat ttatcattta
    14401 tatggaggga aaaaacaact tgctttttcc ataaagaaag caataagaga aaaaacatct
    14461 ctcccctgct ctgctggaat tggaccaaat aaactcatag caaaaattgc ctcctgttta
    14521 tgtaaaccag atggaattaa agttgttgaa aaagaggaag tagaaaaatt tatttgcgac
    14581 ctgcccgttg ataaaatacc tggaattgga gaaaaaacta gagaaaaact tgaagaattt
    14641 ggaatagaaa agtgtaaaga tataagaaaa gttggaaaag aatttttagt tgaaaaattt
    14701 ggaataattg gtgagataat atataaaaaa agttttggta taggaagtca ggaaataata
    14761 aaaattcatc cacctgcaaa atcaattgga cattcttata cattgccttt tgatacttct
    14821 aataaagaaa ttataaatat aattcttttc aaattgtctt ataaagtggc agaaagaatg
    14881 agaagtgaaa ataagcacgg gaaagtaatt actatatcat taagatttga tgatttttct
    14941 tcaattacaa agagaaaaaa atttgtagat attccagcag atggaaaagt aatttttaaa
    15001 ataggcagtt ttattttaga agaaataaag attgaaaaga gaataagagc aattggaata
    15061 actgttggag aaatttttga ttttaactat gactatcttt ttaaaagaga aaacaggaga
    15121 gaatatcttt ttcataaaat tgacgatata aataataagt ttggagaaaa gactatattc
    15181 ccggcagttt tattacttga aaaaaattta gaagtggaaa aaacacattc ttttgttatg
    15241 tggaaatttc agaaaaaaga ttagggaaac taaattcaaa atttctctga ttacctattc
    15301 ccctattatt tttatcaaaa ctctctttgg tcttcttcca tcaaattcac cataaaatat
    15361 ctgctcccat ggaccaaaat caagtttccc atcagttatc gccacaacta cttctctccc
    15421 cataatttgt cttttcatat gggcatctgc attatcttca ccaacattat gagcatattg
    15481 agacaccggt tgatgaggag caagtttttc aagccatttc ttaaaatcat tatgtagacc
    15541 taactcatca tcatttataa aaacactggc agttatatgc atcgcattta caagaacaat
    15601 tccttcttta attccacttt tatcaattgc tttttgcact ctctcggtaa tatttataaa
    15661 ctctactttc tctttcgtat taaaccatat ttcttctcta tgtgttttca ttttatcctt
    15721 tgtcattatc attttatatt ttatattccc tgtgtcaaat aagttgacac aggacaatta
    15781 tttatctata atattttagg gtctcagaaa ttaataattt ttggaaaata caagaaataa
    15841 atatgtttaa accaggagga gaaatgaaga agataactgt ttttgtgatt tgtcttttat
    15901 tttttatttc ttcaaatctt tttgctttat caaaagaatc tcttgttgaa agaattaaaa
    15961 tggcagaaaa attacttgat gattttacga ctgctcctga tagacaaatt ccacaggaat
    16021 taatcaacca ggcagaagga attattttta tgaaacaata taaaggtggc tttgtacttg
    16081 gagcaaaagg aggaaatgga ataattcttg caaaagacag aaatacagga aaatggagtg
    16141 cccctgcttt tgtctctaat gttgaaggga gtttcggatt tcaagcaggt ggtcaatcaa
    16201 ttgaaacaat tgttttaata atgaataaag aaggacttga tatgcttctt aaatcaagag
    16261 taaaaattgg agttgacatt tcagcagcag caggtcctta tggtagagat gcagcagcac
    16321 aacttggagc aggagttggt ctccttatat attcaagggc aaaaggactt tatgccggtg
    16381 cttcaataga aggtggattt cttgcaattg atgaagaagc aaatcaaatt ttctatggga
    16441 aagatgttaa agtaagggac attttaataa gaagaacagt aaaaatacca gaagaggcaa
    16501 aaacactcat acagaaactt gaaaattatg cacaaattac tgaataactg tttaacttcc
    16561 ctttaaggaa tttttaattt ctgcccaaca taaactgtat cattctttaa attatttagt
    16621 ttttttatag cactgatcgt tgtattatgc tttcttgcaa tttcactaag attatctcct
    16681 tttttaacaa tataatattt tacatcagaa gatagttctt tagatacttc ttttgaagtt
    16741 tctttttcag aaatttgtgt tgttccactc tgtgaaattt tatttttaag aaaataaatc
    16801 tcactttcat gctttgttaa ttcatcaaga aatatctttg aatttttgtt taatagttct
    16861 tcaagttctg aatattttaa tgtattatca cttataattt cttcctgtga tttctttaaa
    16921 acagataaat ccttctgcat taaagtaaga gaactgttta atgttgcaat agaagaaata
    16981 atatctgtat atgtcttttt gagtgaatct atgtctctcc tcacttctat ggtatttttt
    17041 tctatattat cattctgttg ttctaccttt ttttcaaagt tataatttac ttcatctatt
    17101 tttcccttta ttagattaac ttctttttca atagttgaaa ttctttctaa aattattgcc
    17161 tggtcttttt taaatgattc aacttttaca ttaaggttgt caattttatt attatagtca
    17221 ttttcaattt ctttaaactt atctgctatt gcttttgaag ttaaatataa atcctgctca
    17281 acagaagtaa ccttttcttt tactgtacca acatcttctt ttgttgcaat tgactgacaa
    17341 ccggcaaaaa taaagggtaa gaataataaa agtaataatt tctttttcat tttacaattt
    17401 taaattcaca tcttctattt tttgcccatg cttcttcaca atgacgaggg tctgctgggt
    17461 tgtcttctcc ataacttaca gtataaagac gacttggaga aattccatag ttaattagaa
    17521 aatttcttgc acttaatgct ctctgttcac caagaactaa attatattcc cttgtccctc
    17581 tttcatcaca atgtccttca ataagaattt ttacttctgg atttgctttt aaataatctc
    17641 ctattttctt taatttttct attgagtcct gctttaaatc atatttatca aaattaaaat
    17701 aaatattttc aaatattgcc gcaacttctc cttctggttt ctctactttt gcaccttcag
    17761 ataatttttg aggtggtact tcttcaccaa cagcaacttt aacttcttct ttcttctctt
    17821 ctacttttgg tttttcaact ggtttttcaa tttcttcaat cactttgcta attggagtcg
    17881 cttctttaac ttcttctttt ttaacctctt ctttcttggc ttcttttttc cttccaggac
    17941 actgacaact cgcaaaaagt accgctacta aaaaaactaa accaaacttt aaatatttca
    18001 tcataccctc ctttttaagg aattatatat ttcacttttt ttattattat attcttcact
    18061 ttttaaattg tcaaattttt taaaattttt cccaatcagg actgaaagta tcatatttaa
    18121 aattagtaag gtctcttgtt tcccctgtaa aaacatctat tatacatatt tttgatggat
    18181 atatattcac ttttgaaaaa acaatatgtc tgctatctga agcccatgaa attgtttcac
    18241 tgccagaaga atttgataat atttttgttt tatttgtttt tacatcaaaa attgcaattt
    18301 tataatcacc agttctaaca atatatgcaa tataattccc atccggagac catttaggtg
    18361 atgttgagta cgagaactca taacttattc tttttactcc aaatccatcc gaatccataa
    18421 taaaaatttg gggtgttcca tatttgtcag aaacaaaagc aattttttca ccatcaggag
    18481 aaaatgaagg agaactatca acccctttat aatttgttaa tcttcttatt attttcccat
    18541 caattgttgc taaataaatt tctggattac ctgatttact taaaacaact cctaaaatat
    18601 ttttttgctt acagggtgct acacatgcat tcaaaccagg gtactggaaa attgtttctt
    18661 cttttcctgt attaaaatca tattttgcaa tttcaggata attatttttg taagaaacaa
    18721 aataaaatcc acttctatca cctgtccaaa ctggaaaatt aacaagaaaa ttaaaattag
    18781 taatttgctt taaattcctg ccatcataat cacaaacata tatttgatat tttccagttt
    18841 tatttgcaac aaaaataact ctactctggg caattcctgg ctttccagta agaaataaaa
    18901 caatatcatc gttaacaaca tgagcaaaat aaaaggggtc tttcaaaata ttataatttt
    18961 tcctaaaaag ttcttgttct tctaaattat caaaaacaga gacaacaata tttccctttt
    19021 catttttaac cgaaattata atatttactg gaaagtcctg tttactttcc tcaatatctt
    19081 caacaaaatt ttcacttttg atattaaaat atccagaata aaaaaggtct ttactcaatg
    19141 tatttgaaaa attttcccag tagttatttt tttcaatttg aaaaagtaaa acaacctctg
    19201 ctttttgagt aaaatccttt gttatttgaa gaaaaacttg tggaacagaa aaacacggca
    19261 ttataattaa gaaaaaaaac aaaagaaatt ttctcattac ccctcctctt ttttaaactc
    19321 tattgtcact tcaagatact cctcttttat ttcctcagga aatggaggaa tattttttgt
    19381 agattttatc gcactttctg ctgacttgtc aaatggtttg tacccagaac ttttttcaat
    19441 tcttaaatta gatatttctc catttttata aatcctaaaa gaaattattg tagaaaagtc
    19501 accaataatg ttaccaaagt tccagttttc ttcaatctta tttttcaaaa gtgatatata
    19561 ccaggaaggt atatcatttg aaattcttaa attctctaaa tttaatgatg tagaaaaaag
    19621 agttgacata tcttcttggc ttatttcctg tttaatattt gttttattgc tactaccttt
    19681 tgtattagaa ctacttattt ctcgatttcc aattttgctt aaaagttctt ttttatattc
    19741 atcagcatta aaagaagatg tcttactact tggagaaatt tcaccagcag aagaactttt
    19801 cctccctgaa gaaccttttt tttcagaaga aatttttgtt ttttcttgat atttttcttt
    19861 tacctctttt tttacttctt tttcctcttc agttttataa ttttctccaa caggggtgtt
    19921 aattacctgc ataactcccg gaatatcaac tatctgtaca aaaaaaacat tctctttcct
    19981 cttcatattt acagaagaaa aaatcaaaaa aacacccgca acgatatgaa ttacaacaga
    20041 taataagaaa ctcctttttt ttactttacc tgtgtcgcca aacctacttt ctctatttcg
    20101 cattccttta ttatatctaa aactttaatt atgttattat aagagacatc tttatcacct
    20161 ttaacaatta cagaaatacc tgtccctttt ttttctttta attccttata taggtctctt
    20221 aatgtaattt ttgtacttcc caaataatat gtttcatctt ttgcaacagt tataactaca
    20281 ctcttttcta cttctacttt tgatggagat gattctggca atttaacttc aatcccctgt
    20341 tcaattaaag gtgccatcat caaaagcatt attacaagat ttaaagcaac atcaacaaga
    20401 tttgtaatac ttatatttga aagtatagaa ctatttttat tatcaaaata gttattcatt
    20461 tttaattttc aaataattta aaatatctcc tgttatactg aacatatctg ttttaatttt
    20521 ttctgtttta tcctgaaaat aattataacc aactgctgct ggaattgcta caagcaatcc
    20581 ataaacagta gttataagtg cttctgcaac tcctgatgaa attactctgg tagaagcaga
    20641 accagtagca gcaatattat taaatgccat tagaagtccc caaacagttc ctagcaatcc
    20701 cagaaaaggt gcaatagaag taattgttgc aagaaaagaa aggtctgttt ttaatttatt
    20761 tgattcaata aaaattattt tttcaatctc ttcttcctta atatccccct ttttatcaat
    20821 tatcctttta cattccttta aaacaaaaaa aagagggcat ttaataacta taattttttt
    20881 accataagta attatatctt tcataatatt attttttctt tcaacactcc ttaaataaaa
    20941 aaatttatag aacataatat accaggaata tattgaaaga aaaagaagaa taacaatatc
    21001 aagttttcca gcccaatcag ataattgcca ggaagaaacc catatattac ccatatttac
    21061 tcctattcct ctttctttta actcttttct ttattccacc agtttatcca ttcttctcta
    21121 gcttcaaaat ttttatttgt tatttttttc aaaacatata ttaaattttt ttcaattgtt
    21181 ttattcaact ctatataagg atgtttatct tcaagaacct gaaaaatata ttctaaagat
    21241 ggctttccta tttttattat tgcttctatt gcctcttttg caggagaagt ccatacccct
    21301 tttccatcat atttacacca tgtaaactcc tcatcatcaa gtaataatat aagaaaagac
    21361 actgacctgc tatttttaat ttcagaaagg ttatatgcac caagagttct tttattaaca
    21421 tcaacagaaa atgtcttata gacattactt cttaccggct cttttaaacc atcaaatttt
    21481 tcttcaccat aaagaagtcc aattataata acaaaaataa aaaatatttt tttcattttt
    21541 tcattttacc acaattttaa aaatcccaaa atttatttca gaaaatcttt aattttattt
    21601 attaaaatga taaaatataa ctatgggaaa aaaactaatt attttaattt tctactttgc
    21661 ttttataccc tcaatttttt gtttaagtaa agaagattac aatgtaattc tcaaaaggaa
    21721 tatctttaca actccctata ttgaagaaaa agagataaaa aaagaaagta tattgaaacc
    21781 accacctcca ccatcattaa acactcttat aaaagcaaaa ggagttatat tgataggaga
    21841 aactgggtat ggaattgttg aagtgaaagg taaagatatt attgtaagaa aaaatgaaaa
    21901 aatacttggt gcagaagtag tcaaaataga aaatgattct attgtattct tttataatga
    21961 caaagaagaa aaattacaaa ttgaaaaaat tacagaaaaa gggaattttg tgaaagcaac
    22021 tcctgaaata aatgttaaaa tagaggaaaa tataaacact aataaagagg agaaagaaat
    22081 atctcagcca gaatttaaag aaccagttgt agttgatttt gaaaaaacaa tttcagaatt
    22141 gaaaaacgat aaggatttgt ttaacaatgt gaatatcatc ccaaatatag aagaagggaa
    22201 tgttaatggt tttaaaataa ataacttacc tgaaaatagt attccatttc aatatggttt
    22261 aagaaatgga gatataattt atagagtaaa tggtattgta attaatagca tgccaaaagg
    22321 acttgctgtt tataatcaaa tagtcaaaag tgggacaaaa actgttacag tagaggttat
    22381 aagaaatgga agtccagttg ttttttcata tacattgaaa tgaaatatcc tcaggaaagt
    22441 taaaaaattt caaccccaaa aaggagtatg ttattattga agaattatta gtaaaaaaca
    22501 aatgtaaaaa aatgaaagaa gcaattttct ggaaaaaaga aaatgacaaa ataagatgtg
    22561 cactctgtaa tcatttctgt ttgataggag aagggaaaac aggtttatgt ggagttcgac
    22621 ttaataaaac cggcgttctt tattcacttg tttatggcaa gacagttgca gaacatattg
    22681 acccaattga aaaaaaaccc tttttccatt ttttgcctgg ttctttttct tattcaatag
    22741 caactgttgg atgtaatttc ttctgtccgt attgccagaa ttatgaaata tcacatccac
    22801 aagaaatatt ttacacaaat caaacaaccc ccgaagaaat tgtaaataaa tcattatctt
    22861 ctaactgcaa gtcaatttct tatacttata cagagccaac tgtttttttt gaacttgctt
    22921 ttgatacatc taaaattgca aaacaaaaag gtattaaaaa taattttgtg actaatggat
    22981 atatgagtaa agaagcacta aaaacaatat ctgaattcct tgatgcagca aatgttgaca
    23041 ttaaaggaga tgaggacttt tacaaaaaac tctgtaaaac aaaacaaaaa tatgtgattg
    23101 acaatatatc tcttatgaaa gaactgggaa tatgggttga aattactact ttaatcattc
    23161 ctgaatacaa tgatagtgaa aaacaactta aagacatagc aaaaataatt aaagatattg
    23221 atattgcaat tccctggcat gtaacaagat tttacccaac atacaaaatg tcagaccact
    23281 atcctgcatc tattgaaaaa ataaaaagag caagagaaat tggatttgaa acgggactga
    23341 aatatgttta tacagggaat atttacattg aaaaaggaga aaatacatat tgtccctcct
    23401 gtaaaaaact tctcatagaa agaagcggat tttcaatttt aaaaaacaat ataaaaaata
    23461 gtaaatgttg tttttgttct gaaagaattg atggtgtctt ttaataactt caaaaaataa
    23521 ggtataatta caaacataat taaaaattcg ggcggttagc tcagtggcaa gagcgctacc
    23581 cttacaaggt agatgtcgca ggttcaagtc ctgcaccgcc cactaaaact ggtaaaaacg
    23641 gatataatgt tgagaacatt gaaagtaata ataactcctg cggctgaaaa ggtatgggaa
    23701 aaaacttttt cccatattta taa
//
LOCUS       DAOOVW010000028        21327 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_1674_length_21327_cov_8.343691, whole genome shotgun sequence.
ACCESSION   DAOOVW010000028 DAOOVW010000000
VERSION     DAOOVW010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 21327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21327
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1674_length_21327_cov_8.343691"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="PLW95_05150"
     CDS             complement(<1..335)
                     /locus_tag="PLW95_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889235.1"
                     /note="catalyzes the formation of acetyl-CoA from pyruvate
                     and coenzyme A; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HOV22049.1"
                     /translation="MPSLKQLSKRNEKFVSGHRLCAGCGAGITARQVMLAIDKPVIVA
                     NATGCLEVASTIFPFSAWNVPWIHSAFENVAATISGIEAAYRSLKKQGKINIEDVVFV
                     AFGGDGGTY"
     gene            complement(345..1544)
                     /gene="porA"
                     /locus_tag="PLW95_05155"
     CDS             complement(345..1544)
                     /gene="porA"
                     /locus_tag="PLW95_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HOV22050.1"
                     /translation="MEKTKNIVAKTGNEAFAEGMRQINPDVVAAYPITPATEIVQIFS
                     QFVADGLVDTEFVAVESEHSAMSACIGASASGARAMTGTSSQGLALMWEMLYIASGLR
                     LPIVMAVVNRTLSSPINIHCDHSDTFGARDSGWIQIYSENVQETYDNIFQALRIAEHP
                     DVKLPAMVTTDGFILSHCLETLEILTDEQVKEFIGIPPERDNLIKSLIENKPITIGAL
                     DLQDYYFEHKRQQAEGMKNALKIIPEIGKEFGDKFGRYYGFFEEYKLDDAEVGIVIIG
                     STTGTAKEVVDQMRKEGKKVGLLKLRVYRPFPAEEIAKVLSKLKVLGVVERVEGMSGF
                     VSPLYSEISSSLYHYEKRPTLVNFVYGLGGRDVKLEDIRDIFDELLKISSTGKKEFTL
                     KYIGVRE"
     gene            1708..2232
                     /locus_tag="PLW95_05160"
     CDS             1708..2232
                     /locus_tag="PLW95_05160"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOV22051.1"
                     /translation="MEKEIKKVIEEIELCLANIKKEYFEILEKNLKENKKIFICGVGR
                     SGLVGKMFAMRLRQIGYESYVVGETITPSSSKNDLVIFISYSGEKQYNIELAKRIKKE
                     GTKILVISSQKKSSLSEISDFKIYIPNKNSVQFGNSLFEQCVFIFLESFILYLREKYK
                     ISEEKFKKGHTNLE"
     gene            2253..3062
                     /gene="proC"
                     /locus_tag="PLW95_05165"
     CDS             2253..3062
                     /gene="proC"
                     /locus_tag="PLW95_05165"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOV22052.1"
                     /translation="MKIGIIGFGNMGSIFAESFCSYGYFKKEDIFVSDKDKNKISIAK
                     RKDYRIETNTELAKKTDIILLAIKPDDIEKVIEEISGFLDRKKLLISIAAGVKIKKIE
                     GKLKNKNPVIRVMPNLNIKVKAGIIVYCKGKNAKEKKYDVFLKKIFSPLGLVLQMPEN
                     KFDTITAISGSGPGFIFYIADVLTKICEKKKIERKKTISLVKTLFYGSGKLLFYSNET
                     PEELKEMVCSPKGTTIAGIEIFEKNKFPDIFQKVIEKSEKRSKELSKNSKK"
     gene            3484..4302
                     /locus_tag="PLW95_05170"
     CDS             3484..4302
                     /locus_tag="PLW95_05170"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOV22053.1"
                     /translation="MSEWEKVNKAVEYIRSKSKVVPEIGIILGTGLGEFGKTIEDVIV
                     IPYKNIPDFPVSTVESHEGNLLIGKIGNKNVVAMQGRFHLYEGYSAVQISFPIRVMKQ
                     LGIKTLFESNASGGLNPLFERGDIVIITDHINLTGYNPLVGENDERFGPRFPDMSNPY
                     SKNLIELAEDIAIENKIKIKKGVLVGLLGPNLETKAEYRFLRLIGADMVCMSTVVEVI
                     AAVHLGLNVFGVSVITDMGLPDALKPLTFDEIVEIANSSEPKLTKLFKNIIINY"
     gene            4304..4456
                     /locus_tag="PLW95_05175"
     CDS             4304..4456
                     /locus_tag="PLW95_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22054.1"
                     /translation="MKNKKIEENSKKKKRVTIRCRKCLIIDSYDENEKCCHHCGSILF
                     KKVDEV"
     gene            4488..4661
                     /gene="rpsU"
                     /locus_tag="PLW95_05180"
     CDS             4488..4661
                     /gene="rpsU"
                     /locus_tag="PLW95_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020037214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOV22055.1"
                     /translation="MSDFDRIFRQFKRDVEKERIIKEVKKREFYEKPSQIRRRKKARK
                     HRPQSQYSRQRTY"
     gene            4685..5650
                     /locus_tag="PLW95_05185"
     CDS             4685..5650
                     /locus_tag="PLW95_05185"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HOV22056.1"
                     /translation="MNIYTLTLNPSVDYSFYVPEIKFDDINRITHQRIDPGGKGVNIA
                     RMLLKLGEQSIPITFCTKDNSNIYPKILEKEGLIPIYIKIKEKIRNVYNFISEKGEIL
                     RFNEKGPKISYEEKKQLFKKIMCLNYKKGDIIAISGSLPSGFKKDTYKIILNKLKKFQ
                     LITFVDADGDVLKYAVESSPYIIKPNLWELERCMGEKIKNGRKLVSVVGNLLNKNISI
                     IILTLGEKGTFVFTKEQILYGNVPKVKVKSSVGCGDAFIAGFLFSLKNNKNLKDCLRY
                     AIACGTGKAKQEGTKMPDKKEVMGIYNKTTIFDVDYDWVLKNFVP"
     gene            complement(5601..6569)
                     /locus_tag="PLW95_05190"
     CDS             complement(5601..6569)
                     /locus_tag="PLW95_05190"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOV22057.1"
                     /translation="MKKVISLLLKISISGIFLFIVLKKINLSLLSEIFKNSNNFLVLT
                     GLFIFIGTSFLIAFRYFLIVNIYLGKKKPVMYIWKLTMIGLFFNMFLPTSSGGDAVKI
                     FYLIKDDEKKMLPAISVLIDRFIGAITILTMGTFALFFSQINDQRVKIFIFSLTGITL
                     FFYFFLSYRNFAKKLYNPIGKILPSSFDEKLRTTYSLFNFYFKEKKTIFFSLIMSFFL
                     QFLSVVGNFFVAVGLIKEYLPLSIFFIYTPLIWVSTIIPSIGGVGVREFTYVFFFKNY
                     LGEEKAFALSLIVLFSIIIQAIIGYITFLTFKGQNFSKPSHNQHQK"
     gene            complement(6990..7565)
                     /locus_tag="PLW95_05195"
     CDS             complement(6990..7565)
                     /locus_tag="PLW95_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOV22058.1"
                     /translation="MKILRMFVVFLFLTSFLHSETTNKILMIIAFDQFRDEEYAKPRK
                     ILENSGYKIVVASYKTGIAKGMLGSKVKVDISLEDVKVNEYDGVIFVGGSGASVYFNN
                     QTTLKIANEFYNKGKIVGAICIAPVILANAGLLSGKKATCWPSYEKTLKEKGAVWSDK
                     NVEVDGSIITGNGPAAANEFGEKYLKLLKSK"
     gene            complement(7891..8301)
                     /gene="nusB"
                     /locus_tag="PLW95_05200"
     CDS             complement(7891..8301)
                     /gene="nusB"
                     /locus_tag="PLW95_05200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOV22059.1"
                     /translation="MKRRRGREHALKILYMADIRDENPLDLIDEYWLMNGDEEQDVKN
                     FAEKIVKGVFENKENVDKNISSTIVNWDFNRLGVIDRNILRIGTYELLFEEKIPAVVS
                     INEAIEIAKKYGTEDSPKFINGVLHKIKDNIKKQ"
     gene            complement(8358..8825)
                     /gene="ribH"
                     /locus_tag="PLW95_05205"
     CDS             complement(8358..8825)
                     /gene="ribH"
                     /locus_tag="PLW95_05205"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOV22060.1"
                     /translation="MKTIEGNLDARDKKFGIVVSRFNEFITKKLLDGAIDCIVRHNGD
                     EKNIEVIWVPGTVESVYAISKILNSEKKYDAIICLSAVIRGETPHFDYVASQISKAIT
                     QFNMENKVPVIFGVITADSTEQAIERAGTKMGNKGWQAALSAIEMIDLSSKIK"
     gene            complement(9110..9910)
                     /locus_tag="PLW95_05210"
     CDS             complement(9110..9910)
                     /locus_tag="PLW95_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22061.1"
                     /translation="MNKFPRTIVGGISLPRLICGTNWLLGYSHTSSGQNNFIKQYMDE
                     EKVVEIFMTFLKYDIDAVMGPPNDFLVKCIKKAENKSGKKIMYICTPGKIEEVEWAKE
                     NGATFCFPHQSVTDTFVNREKKTMEGIEVWLKKIREFDMIPGLSTHTPETILYADKEN
                     IDVESYIQLYNVLGFLCQVEIEWIHNIIHEAKKPVMTIKPLAAGRISPFPGLVFVWNT
                     IRDIDMVTIGCMSQYEVEEDINLSLSILEKRKTDIELQKTRSKKSFEI"
     gene            complement(9903..11144)
                     /locus_tag="PLW95_05215"
     CDS             complement(9903..11144)
                     /locus_tag="PLW95_05215"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008289105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOV22062.1"
                     /translation="MEEKIFSSIEEAIEDIREGKFVIVVDDENRENEGDLVIAAEKVK
                     PEDINFMAKYGRGLICVALLPERLKELNLPPMAVENTALHGTDFAVSVDASSGITTGI
                     SAYDRAFTIKLLVDKKTKPSDLVKPGHIFPIRGKEGGVLIRAGHTEASIDLSRLAGLY
                     PAGVICEIMKEDGTMARLPDLVEFSKKHNLKIITIKDLIKYRRKREKLVERVVDTFLP
                     TEFGDFILYLYTDKIEGRNHMALVKGDIKLKDENDSVLVRMHSQCITGDIFRSVKCDC
                     GKQLYKSMEMIQKEGRGVIVYLPQEGRGIGITNKIKAYHIQEKEGLDTVEANLKLGYP
                     PDLREYGIGAQILVDLGLKRVRLLTNNPKKIVGLKGYGIEIVERVPIEIEPTKYSEKY
                     LKTKKEKMGHLIKIEGEDENE"
     gene            complement(11640..12200)
                     /locus_tag="PLW95_05220"
     CDS             complement(11640..12200)
                     /locus_tag="PLW95_05220"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOV22063.1"
                     /translation="METIKIKDKKIPYKKVYRKVKYPRLEFKTGNLSVILPENYYGEE
                     QLLEKHKEWVFKKFCFIEETLKEAKKLELKSKVDKKIFTKKVEKIAKEYSSDLTVNVN
                     KIFFRKMKTKWGSCSYDGNLTINKLLMFLPTRFVRYIVFHEVLHRIYRKHNKEFYNII
                     KKKFKNSDKIEKELFSYWFLLTDRKS"
     gene            complement(12336..12680)
                     /locus_tag="PLW95_05225"
     CDS             complement(12336..12680)
                     /locus_tag="PLW95_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22064.1"
                     /translation="MTYTNLTPEIIKDNTIKFPSFLKPCPTHTLEEGIMLVLGVCPRK
                     RFNFRHKVKTPSPHPLPTGERIKREGEQKRKMRSSLFLLSAGVHAQFYCSTIEIPLFI
                     SSPPRGEDYAHP"
     gene            complement(12711..13292)
                     /locus_tag="PLW95_05230"
     CDS             complement(12711..13292)
                     /locus_tag="PLW95_05230"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF006767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOV22065.1"
                     /translation="MFSGIIQATGTIKNKRGKGDITELEIECFPLYEKLQIGDSIAVN
                     GVCLTIEGKKEKCLFVSITKFTENETNLGQLKIRDVVNLEPPLILGDKISGHFLTGHI
                     DFKTKISSFIKSSQTGKIVLDIPQNYRKYFVNKGSIGIDGISLTISEIKGNRVEVNII
                     PYTIENTNLKYKKTGDIVNIEIDILAKYIDALR"
     gene            complement(13277..14404)
                     /gene="ribD"
                     /locus_tag="PLW95_05235"
     CDS             complement(13277..14404)
                     /gene="ribD"
                     /locus_tag="PLW95_05235"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOV22066.1"
                     /translation="MVDHIKLLKQAIKLAEKGKGLVSPNPMVGAIIVQKSKIVGSGYH
                     QCFGMPHAEVEAIKKAGKNTIGATLYVNLEPCCHWGKTPPCTEKIISSGIKEVISCMK
                     DPNPIVNGNGFKILQENGIKVRLFPVEGAFQLNQPYITYITKKRPYIILKWAQTIDGK
                     IATKTGDSKWITGEKARKFVREKRFEIDGIVVGINTVLADNPFLDYIPPQFEVKKKLL
                     ERKRYYKIIIDPHLKTPLESNIWKNEKSKVLIFISDKVDKEKISFYHQKKENFEFVCL
                     STENGKFKIDEFLGQLYKKEIGIIMVEGGKNVLTWFFENRKVDEFLIFIGNKIIGGEN
                     SINVIGGKDIEKISESFYLENRKIEFFDDDILIRGKVCFQE"
     gene            complement(14397..15446)
                     /locus_tag="PLW95_05240"
     CDS             complement(14397..15446)
                     /locus_tag="PLW95_05240"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent dehydrogenase"
                     /protein_id="HOV22067.1"
                     /translation="MKAAVFYGIEDIRIEDFKEPTCNENEVKIKVHYCAICGTDVRIY
                     FSGHKKVNPPAIIGHEITGEIVEIGRNVNYPEIKVGDRITLVTSIGCKKCKMCQKGYY
                     NLCPDTKAIGYYYQGGYAEYLVVPEEAVKQKVILKLPDNVSLIEGTLIEPLSCCINGQ
                     KYLNINDGDNVVIFGGGPIGFMHSVLAKAQGGSKVIIVDPAYERLEKFGKSFPDIILL
                     DGKSNFKEKIMQITDNVGPDVVITACPSKQAQIDGIELLAEKGRISFFGGLPKDDSRI
                     TIDSNTIHYKELSVFGSFASNREDYITALNLISEGKIDAKKFITMTLPLEKITDGIKA
                     VRKGEVLKCVIEING"
     gene            complement(15770..18730)
                     /locus_tag="PLW95_05245"
     CDS             complement(15770..18730)
                     /locus_tag="PLW95_05245"
                     /inference="COORDINATES: protein motif:HMM:NF038162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intein-containing RctB family protein"
                     /protein_id="HOV22068.1"
                     /translation="MENIKKISEYKWIIPKTDGMRVDGVIFISEKLLQKAIEDKAVEQ
                     VINVAKLPGIVKYSLAMPDVHWGYGAPIGGVGAFDIESGVIAPGFVGYDINCLVGETK
                     ILHKYGYYLPIEKCDKKWGEIVCFNKEKTKKEKGEIINFIKRKIYDDIYEIKTETGYK
                     IKATKEHPFWTEEGKIEVKDLKIGDSVMIFPFKGVPYQEMSDETILDIEKFKEVAYQF
                     NKNTNGNSLSQIINQLEKRELLPLKYNSPHLPEILKIIGYILGDGSISFLKDGQGFLI
                     SFSGKSQEDIKEIKEDIEKLGFLPFMNCRKRKHKIKTEYSTYEFETTTYECQTSSRSL
                     ALFLISLGVPYGKKTFSNFYIPDWIFKLPLWCKRLFLSSYFGAEMSSPKWLKNNKFNF
                     YCPIVSMNKEKQYLESGIKFFKQIAKILEEFGVDTKKISVKEIKTGENNITKFRIRLI
                     LSSKLENLLNLYEKIGFEYNREKEFFGNIFTFYLRKKKNILKERGKAEKVAICLKNAG
                     YSFESIYSKTQSEWVNRRFIERSVYEGRKTEPRISFSFNGIFEILNEVRTKNGESGFV
                     FDKIVEIKKIQYEGYVYDLTINHKSHNFVGNNFLVSNCGVRLIRSNLTRDDIEDKLSE
                     LANGLFYNVPSGVGSTGKLKLKRKELEKVIVEGAKWAVKNGYGIEDDLENMEEYGCME
                     GADPSVISNRAYERGLPQSGTLGSGNHFLEIQQVVNIYNKEIAEKFGIFEGQITIMVH
                     TGSRGFGYQICDDYLDKFGKVVLKYGIKLPDRQLACAPFSSPEGKRYFSAMKAAANYA
                     FANRQVITHWIRETFSRILKQPWEKQELFVVYDVAHNICKLETHNVDGRMKKLLIHRK
                     GATRAFPKGHPSLPEKYKDVGQPVIIPGTMGTASYILVGTEKALEETFGSTCHGAGRT
                     LSRHQAIKQAKGREIDVELSKKGIIAKSVSRKGLAEEMPEAYKDVDEVIKVVEGAGIS
                     EKVAKMIPIAVIKG"
     gene            complement(18718..19263)
                     /locus_tag="PLW95_05250"
     CDS             complement(18718..19263)
                     /locus_tag="PLW95_05250"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOV22069.1"
                     /translation="MRIVIGGTGGQGILTLGKVISYACINRDLDVSCFPVYGAEMRGG
                     YVHCILTISNAENILSPISSECDVGIFMEETSFNILSSYLKNEGIIINNSSLIKNNNK
                     GSVYQFPATEQAEKIGNSKVSNMFFAGLVIPFIKKFFNEFKKEDMFYGVDKVIKEQKM
                     RKLSKEAIEIGFEYAIEKWKI"
     gene            complement(19278..20402)
                     /locus_tag="PLW95_05255"
     CDS             complement(19278..20402)
                     /locus_tag="PLW95_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22070.1"
                     /translation="MKLDGKTIYAQSSDIKSRTYLEYRKDMKKKAIAELEILEWLENK
                     VKEMFPRKQVKVYKSGGDKFLWFLRKGGISRDPDFIAEINDGKIGFEFQYTEKSNLKF
                     YDFKISKVAKKKGDQYKPIEGKLFVYIHRPLLKYAIFKPELIIKNGEREMVEAWRSYG
                     FRVPKEKFEKLLKSDTALKNVCQRIDIKNFILNFQHELIDINREKLSILLQSVIDENN
                     IVKIIPKDLESFFKVCFILDNLNKIPQNINLWFVYLLSYVNKNILLEDISKIVYCIDF
                     LYSKTELHPNELILLIKKVRELMLMIKESYQNDGSYKSSIKASPVDETRYALFSINLL
                     EDLIQDMIYYYSVTELKPVKKIYENLENIEKTYELIKSAK"
     gene            complement(20412..>21327)
                     /locus_tag="PLW95_05260"
     CDS             complement(20412..>21327)
                     /locus_tag="PLW95_05260"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOV22071.1"
                     /translation="MRFDLKELKKYEEKFKNKFHTNKIKVNDVNIIEINGTIQKIFVK
                     NSMLMSDLPDNSTHLMITSPPYFDTKMYSRKPIENDLGNIHDIDEWFDKISIVWKEVY
                     RVLQPGRKAFINIMNLPLRLGNGSFKTLNLVGRTIDICEKIGFIFKRDIIWHKTNAVR
                     AHFGTYPYPGGILINNMHEFILEFDKPEKKGFNKYGHLSKEQKEQSKLDKEFWLSIKK
                     SDVWVMKPQGSGDRRNHIAPFPYELPYKLIKAFSYIGETVLDPFVGSGTTLLASADLK
                     RNGIGYEIVPEIANDAIICLKNYQPKLW"
BASE COUNT         6842 a         3696 c         2872 g         7917 t
ORIGIN      
        1 tcataagttc ctccatctcc accaaaggca acaaaaacaa catcttcaat atttattttc
       61 ccctgttttt ttaatgacct atatgctgcc tcaattccac ttattgttgc tgcaacattt
      121 tcaaaagcag agtgtatcca tggaacattc catgcagaaa atgggaaaat tgtgcttgct
      181 acctctaaac agccagttgc atttgcaaca attaccggtt tgtcaattgc aagcataacc
      241 tgtcttgcag taatacctgc tccacaacca gcacataacc tatgtcctga aacaaatttt
      301 tcatttcttt ttgaaagttg ttttaaactt ggcatttttg cctcctattc tcttactcca
      361 atgtatttta atgtgaattc ttttttccct gtacttgaaa tttttaaaag ttcatcaaaa
      421 atatctctta tatcctctaa ttttacatct cttccaccta atccatagac aaaattaacc
      481 agtgttggtc ttttttcata atgataaaga gaagatgaaa tttcagaata aagaggtgaa
      541 acaaaaccag acattccttc aactctttct acaactccaa gaacttttaa tttagataga
      601 acttttgcta tttcttctgc tggaaaaggt ctataaactc ttaattttaa aagtccaact
      661 tttttccctt cttttctcat ctggtcaaca acttcttttg cagttccagt agttgagcca
      721 attataacaa tcccaacttc tgcatcatcc aatttgtatt cttcaaaaaa cccataatat
      781 cttccaaact tatctccaaa ctctttccct atttctggaa taattttcaa agcatttttc
      841 attccttctg cctgttgtct tttatgttca aaataatagt cctgtaagtc aagagcacca
      901 attgttattg gtttgttttc aattaaactc ttaattaaat tatccctttc aggagggatg
      961 ccaataaatt ctttaacctg ctcatctgta agtatctcca atgtttctaa acaatgactt
     1021 aaaatgaacc catcagtagt taccattgca ggaagtttaa catcaggatg ttcggcaatt
     1081 cttaatgcct gaaaaatatt atcatatgtt tcctgaacat tttcagaata aatctgtatc
     1141 catccagaat ctcttgctcc aaatgtatct gagtggtcac agtggatatt tattggggaa
     1201 gagagagtac gattaacaac tgccattaca attggtaatc tcaatccaga agcaatataa
     1261 agcatttccc acattaaagc aagtccctgt gaagaagtcc ctgtcattgc tcttgcccca
     1321 gaagcagatg caccgataca tgcactcatt gcagaatgtt cactttcaac tgcaacaaat
     1381 tccgtatcaa caagtccatc tgcaacaaat tgagaaaaaa tctggacaat ttcagttgct
     1441 ggagtaattg gataagcagc aacaacatca ggattaattt gtctcatacc ttcagcaaat
     1501 gcctcatttc ctgtctttgc aactatgttt tttgtttttt ccatctcttt ctccttttat
     1561 tttcaagtca aaactgttct atttccattt ttccctaaat taaacagggt tttaaaagaa
     1621 atgagataat atgattttga tatatatttt tgataaaagg atataataaa acttttaatt
     1681 tgtcaaattt ttagataaaa tagaagtatg gaaaaagaaa taaaaaaagt tattgaagaa
     1741 attgaattat gccttgcgaa tataaaaaaa gagtactttg aaattttaga gaaaaattta
     1801 aaagaaaata aaaagatatt tatatgtgga gttggaagaa gtggacttgt tgggaaaatg
     1861 tttgctatga gattaagaca aattggatat gaaagttatg tagttggaga aacaattact
     1921 ccatcatctt caaaaaatga cttagttatt tttatttctt attcaggaga aaaacaatat
     1981 aatattgaat tagcaaaaag aataaaaaaa gaaggaacaa aaattttagt aataagttcc
     2041 cagaaaaaaa gttctctttc tgaaatttct gactttaaaa tttatatacc gaacaaaaat
     2101 tcagtccagt ttggaaattc tctttttgaa caatgtgttt ttattttctt ggagtcattt
     2161 attctttatt taagagagaa atataaaata agcgaggaga aatttaaaaa agggcataca
     2221 aatttagaat aaattatgat aaaattttga aaatgaaaat aggaataatt ggttttggta
     2281 atatgggcag tatttttgct gagagttttt gttcatatgg gtattttaaa aaagaagata
     2341 tatttgtttc tgataaagat aaaaataaaa tctcaattgc aaaaagaaaa gattatagaa
     2401 tagaaacaaa tactgaattg gcaaaaaaaa cagatattat tcttttagca ataaaaccag
     2461 atgatataga aaaagttatt gaagaaataa gtggatttct ggatagaaaa aaactcttaa
     2521 tttcaatagc agcgggagta aaaataaaaa aaattgaggg gaaattaaaa aataaaaatc
     2581 ctgtaataag agttatgcca aacttaaaca ttaaggtaaa ggcaggaata attgtctatt
     2641 gtaaagggaa aaatgcaaaa gagaaaaaat atgatgtctt tcttaaaaaa attttttctc
     2701 cattaggact tgttttacag atgccagaaa ataaatttga tacaataact gctatttcag
     2761 gtagtggtcc tggttttatt ttttatattg cggatgtgct tacaaaaata tgtgaaaaga
     2821 aaaaaataga aagaaaaaaa acaatttctc ttgtgaaaac gcttttttat ggaagtggga
     2881 aactactttt ctattcaaat gaaacaccag aagagttaaa agaaatggtc tgttctccaa
     2941 aaggaacaac catagcggga attgaaattt ttgaaaagaa taaatttcct gatatttttc
     3001 agaaagtaat agagaaaagt gaaaaaagaa gtaaagagct ttcaaagaat agtaaaaaat
     3061 aaaattttag tataacaaaa ggggaaaaaa aatactcctt cccttactaa ggtttacccg
     3121 tctatctttg tagaggggaa ggttaggctc gcccgttggg tcctcgcttt tggctacgga
     3181 tcctgtggac tatgcccgag ggatggattt atttaaatcc cccttcatcc ccctttttca
     3241 aagtgggaag tatcactcct tcctttataa aaggaaggac gggatggatt gtcttacaca
     3301 gaagggttta gagttttgct cacagaccat ccctacaccg aagcgataag ttatgcttac
     3361 gggattttag aagaggtatg cttatacaat tttgagagaa cttttaaaaa cttatccaaa
     3421 ggatccttcg gataaaaact ttaatcaatt ttggtaaaac ctattataac atagggggaa
     3481 aaaatgagtg aatgggaaaa agttaacaag gcagttgagt atataaggag taaaagtaaa
     3541 gtagttcctg aaattggtat aatacttgga actggacttg gagaatttgg taaaacaata
     3601 gaagatgtaa ttgtaattcc atataaaaac atacctgatt ttcctgtctc aactgttgaa
     3661 tcacatgaag ggaacctgtt aattgggaaa ataggaaata aaaatgttgt tgcaatgcag
     3721 ggtagatttc atttgtatga gggatatagt gcagttcaaa tatcttttcc cataagggta
     3781 atgaaacaat tgggtataaa gacattattt gagtcaaatg cttcgggagg gttaaatcct
     3841 ttatttgaaa gaggggacat tgtaataatt acagaccata taaaccttac aggatataat
     3901 ccattggtag gtgaaaatga tgaaagattt ggaccaagat ttcctgatat gagtaatcct
     3961 tattcaaaaa atttgataga gttagcagaa gacatagcaa ttgaaaataa aattaaaatt
     4021 aaaaaaggtg tgttggttgg acttttagga cccaatttag aaacaaaagc agaatatagg
     4081 tttttaagat taattggggc agatatggtt tgtatgtcaa ctgttgttga agttattgca
     4141 gcagttcatt tgggattaaa tgtttttggc gtttctgtaa ttacggatat gggactacct
     4201 gatgctttaa aaccacttac ttttgatgaa atagttgaaa tagcaaattc ttctgaacca
     4261 aagttgacaa aattatttaa aaacattata ataaattatt aaaatgaaaa ataaaaaaat
     4321 agaagaaaat tcgaaaaaga agaaaagagt tacaataaga tgcaggaaat gtttaattat
     4381 tgatagttat gatgaaaatg aaaaatgctg tcatcattgt ggaagtatcc tttttaaaaa
     4441 agttgatgag gtttagaatc ttattgaaag agaggtgaaa caattaaatg agtgattttg
     4501 acagaatttt tagacaattt aaaagagatg ttgaaaaaga aagaataatt aaagaggtaa
     4561 aaaagagaga attttacgaa aaaccaagtc aaataagaag gagaaaaaaa gcaagaaaac
     4621 acagacctca atcacaatat tcaagacaaa gaacttatta gacaggtaag aaggaaaatg
     4681 atgagtgaat atttatacat tgactttaaa tccaagtgtt gattattctt tttatgttcc
     4741 cgaaataaag tttgatgata ttaacagaat aactcaccag agaattgacc cgggagggaa
     4801 gggtgttaat atagcaagaa tgcttttaaa attaggtgaa caatcaattc ctataacttt
     4861 ctgcacaaaa gacaattcaa atatataccc aaaaattctt gaaaaagaag gacttattcc
     4921 tatttatatt aaaataaaag aaaaaataag aaatgtttat aattttattt ctgaaaaagg
     4981 tgaaatttta agatttaatg aaaaagggcc aaaaataagt tatgaagaaa agaaacaact
     5041 ctttaaaaaa attatgtgct tgaattataa aaagggagat ataattgcaa ttagcgggag
     5101 tttaccatct ggttttaaaa aagatacata taaaataatt ttaaataagt taaaaaaatt
     5161 tcaactaata acttttgttg atgcagacgg agatgtttta aagtatgctg ttgaaagcag
     5221 tccatacata ataaaaccaa acttatggga attagaaaga tgtatgggtg aaaaaataaa
     5281 aaatgggaga aaattagtca gtgttgttgg taatttatta aataaaaata tatcaattat
     5341 aattttaact cttggagaaa aagggacttt tgtatttaca aaagaacaaa tactttatgg
     5401 gaatgtgcca aaagttaaag ttaaaagttc ggttggttgt ggagatgctt ttattgctgg
     5461 atttttattt tctttgaaaa acaataagaa tttaaaagat tgtttaagat acgcaattgc
     5521 atgtggaaca ggtaaagcaa aacaagaggg aacaaaaatg ccagataaaa aggaagttat
     5581 gggtatatac aataagacga ctatttttga tgttgattat gactgggttt tgaaaaattt
     5641 tgtcccttga aagtaagaaa agttatataa ccaattattg cctgtattat aatagaaaac
     5701 aaaacaatta aagaaagggc aaatgccttt tcttctccta aataattttt aaaaaagaaa
     5761 acatatgtaa attctcttac tcctactcca cctattgaag ggataattgt agaaacccag
     5821 ataagaggag tataaataaa aaatatagat aaaggtaaat attctttaat taaaccaacg
     5881 gcaacaaaaa aatttcccac cactgaaaga aattggagaa aaaaactcat aataagtgaa
     5941 aaaaatattg tctttttttc cttgaaataa aaattaaata gcgaataagt agtcctcaat
     6001 ttttcatcaa aagatgatgg aagtattttc ccaatagggt tataaagttt tttagcaaaa
     6061 tttctataac tcaaaaagaa atagaaaaaa agtgtaatgc cagtcagaga aaaaatgaaa
     6121 atttttactc tctggtcatt aatttgtgaa aaaaataaag caaaagtccc catagttaaa
     6181 attgtaatag caccaataaa tctatcaata agtactgaaa ttgcaggcaa catttttttc
     6241 tcatcgtcct taataaggta gaaaatttta actgcatctc ccccagatga tgttggtaga
     6301 aacatattga aaaaaagtcc tatcatagtt aatttccaga tatacattac aggttttttc
     6361 ttacctaaat aaatattaac aattaaaaaa tatctaaaag caataagaaa ggaagtccca
     6421 ataaaaataa aaagtcctgt taatacaaga aaattatttg aatttttaaa aatttcagaa
     6481 agaagtgaaa ggtttatttt tttaaggaca ataaataaaa aaataccact tatagatatt
     6541 tttaatagca gtgagattac ttttttcatt tgtatatgtt caatgaattc cgttagtaag
     6601 gcacagaaat taaagatatt tcccctcatc tttcttctcc cccagaggag aagaaaataa
     6661 aaaaggcagt aaaatcccct ctttcctatc ttttccctct cccctttggg gagagggaaa
     6721 gggtgagggg ggaatgcacc aactcagagg cacaaggaag tatctttagt cggttttaca
     6781 aagaaaggga ataaaaattc tatcctttgt taactgataa gtaattcccc tctttagggg
     6841 ggcttttttc agcaaacact actggagtta aagtgaattt tgctttgttt tacctctgtg
     6901 cctttgtgtc caatggatcc tgcgatttat cccttccctt gatattcttt tactttgtgc
     6961 ttttcagaca acatagttga ctgagtcttt tatttagatt taagcaattt cagatatttt
     7021 tcaccaaatt catttgcagc agcaggacca tttcctgtaa ttatagaacc atcaacttca
     7081 acatttttat ctgaccagac cgctcctttt tcttttaatg ttttttcata agagggccag
     7141 catgttgctt ttttacctga aagaagccct gcatttgcta aaattacagg tgcaatacat
     7201 attgctccaa caatcttgcc tttattataa aattcatttg ctatttttaa agttgtctgg
     7261 ttattaaaat atactgaggc accacttcct ccaacaaaaa taaccccatc atactcatta
     7321 actttaacat cttctaatga tatatcaact tttactttac taccaagcat accttttgca
     7381 attccagttt tatatgaagc aacaacaatt ttataaccac tattttcaag tatttttctt
     7441 ggttttgcat attcctcatc cctgaactgg tcaaaagcaa ttatcattaa aattttatta
     7501 gttgtttctg aatgcaaaaa tgaagtaaga aaaagaaaaa caacaaacat tcttaaaatt
     7561 ttcattttat ccccctcttt ttggcggcgg ttctaccaaa attggttaat atcttttata
     7621 atgcctttct ttgtctttta atgtttttat aagtatttat ttatatccct tccccttttt
     7681 tctttcttcc ctctcccctt gggggagagg gaaagggtga ggggggcttg tgttctttct
     7741 tcctctttta tcttcctttt ataaaccgac taaagttgct taccttgttt ctcagagttg
     7801 gtgcttgatt tccccctttg taaaaggggg tagggggatt tttatttccc atttctcttt
     7861 catctaattt tttgatacta cctaaaattt ttattgtttt tttatattgt cttttatttt
     7921 atgtaatacc ccatttataa attttggtga gtcctctgtt ccatattttt ttgctatttc
     7981 tattgcttca tttattgaaa ccacagcagg tattttttct tcaaaaagta attcataagt
     8041 gcctattctt aaaatatttc tatctataac acccaatcta ttaaaatccc agttgacaat
     8101 tgtagaagaa atgtttttat caacattttc tttattttca aaaacccctt ttacaatttt
     8161 ctctgcaaaa tttttaacat cctgttcttc atccccattc ataagccaat attcatcaat
     8221 taaatcaagc gggttctcat cccttatatc agccatatac aaaattttca aagcatgctc
     8281 tctacctctt cttcttttca tttaattttc aagttttcct ctacacaagg tcctccagac
     8341 caggtctgga gaaagtatta cttaattttt gaagataggt ctatcatttc aattgctgaa
     8401 agtgctgctt gccatccctt gtttcccatt tttgttcctg ctctttctat tgcttgctct
     8461 gtgctatcag cagttataac tccaaatata acagggactt tgttttccat attaaattgg
     8521 gttattgctt ttgatatttg tgatgcaaca taatcgaaat gaggtgtttc ccctcttata
     8581 acagcagaaa gacagattat agcatcatat tttttttcgg aatttaaaat tttactgata
     8641 gcatatacac tttctactgt tccaggaacc catataactt caatattttt ttcatctcca
     8701 ttatgcctga caatacaatc aattgcgcca tcaagcaact tttttgtaat aaactcatta
     8761 aatctactaa caacaatccc aaactttttg tctctggcat ctaaattccc ttctattgtt
     8821 ttcatatctc cccctttcta attttcaggt tctgtcaaaa ttggctatag cttttataat
     8881 ccttttcttt gtcttttaag gtttttatat gtctttatat ttcccctcat cataatcttc
     8941 tccccacagg ggagaagaaa aaaggaaggg agcatttcct ctctttccgc ctctcccctt
     9001 tttctttctt ccctctcccc accggggaga ggctcgttta tgaatctacg cccgaggggt
     9061 tagggtgagg ggggttttca tctctttttt catctatttt ttgacactac taaatctcaa
     9121 atgatttttt acttcttgtt ttctgtaatt caatatctgt ttttcttttc tcaagtattg
     9181 aaagtgaaag attgatatct tcctcaactt catattgact catgcatcca attgtcacca
     9241 tatcaatatc tcttatcgta ttccatacaa aaacaagtcc tggaaaagga gaaattcttc
     9301 ctgctgctaa tggttttatg gtcataaccg gcttttttgc ttcgtgtatg atgttatgaa
     9361 tccattcaat ttcaacctga cacaaaaaac ctaaaacatt gtaaagctgg atataacttt
     9421 caacatcaat gttttctttg tcagcataga gaattgtttc tggtgtatgg gttgaaagac
     9481 ctggtatcat atcaaattcc cttatttttt taagccagac ctcaattcct tccatagtct
     9541 ttttttctct attaacaaat gtgtcagtaa ctgattgatg tggaaaacaa aaagttgctc
     9601 cattttcttt tgcccattca acttcttcta ttttcccggg agtacaaata tacattatct
     9661 ttttcccact tttattttct gcttttttta tacattttac aaggaaatca tttggtggtc
     9721 ccataactgc atcaatatca tactttaaaa atgtcataaa aatttcaaca actttttcct
     9781 catccatata ttgttttata aaattatttt gtccagaact tgtatgagaa taacctaaaa
     9841 gccaattagt tccacatatt aatcttggta aagaaatacc acctactatt gttcttggaa
     9901 atttattcat tttcgtcttc tccttctatt tttattagat gtcccatctt ttcttttttt
     9961 gttttaagat atttttctga atattttgtt ggttcaattt caatagggac cctctcaaca
    10021 atttcaatcc cataaccttt cagtcctact atttttttgg gattatttgt aagtaatcta
    10081 actcttttca gtcctaaatc aactaaaatt tgtgctccaa ttccatattc ccttaaatca
    10141 ggtggatacc ccaattttaa atttgcctca actgtatcaa gtccttcctt ttcttgaata
    10201 tggtatgctt ttattttatt tgtaattcca atacctcttc cttcctgtgg taaataaaca
    10261 attacacctc tgccttcttt ctgaatcatt tccattgact tataaagttg ttttccacaa
    10321 tcacatttta cagacctgaa aatatctcct gttatacatt gagaatgcat ccttacaaga
    10381 acactatcat tttcatcttt caattttata tcgcctttca caagagccat atggtttctt
    10441 ccttcaattt tatcagtata aaggtataaa ataaaatcac caaattcagt aggcaaaaat
    10501 gtatcaacaa ctctttcaac aagtttctct ctttttcttc tgtatttaat taaatccttt
    10561 atagttatta tttttaaatt atgtttttta gaaaattcaa caaggtcagg caaccttgcc
    10621 attgtgccat cttctttcat aatttcacat ataacacctg ctggatataa tcctgcaaga
    10681 cgggaaaggt caatagatgc ttcagtatga cctgccctta taagaactcc tccttcctta
    10741 cctcttattg ggaaaatatg ccctggtttt acaaggtcag atggttttgt tttcttatca
    10801 actaaaagtt taatagtaaa tgctctgtca taagcagaaa tacctgttgt tattccagaa
    10861 gaagcatcaa cacttactgc aaaatcagtc ccatgtaaag cagtattttc tactgccatt
    10921 ggaggtagat ttaattcctt taacctttcg ggaagaaggg caacacaaat aagacctctc
    10981 ccatattttg ccatgaaatt tatatcttct ggttttactt tttcagcggc aataacaagg
    11041 tctccctcat tttctctgtt ctcatcatca acaacaataa caaatttccc ttctcttata
    11101 tcctcaattg cttcttcaat acttgaaaaa attttttctt ccattttatt attttaccac
    11161 atttttataa aagaatttat agcaaaagtt caaaatctcc ccttatccag ttttgtctgt
    11221 taaaaattcc atttataaaa tcccccctca cccccacctt ctccccatag gggagaggct
    11281 cgcccgtagg gctacgcccg aggggaaagg gtgaggggaa aatatttact ctatatccgt
    11341 tgtcaaattg gtattaatct ctttaaaaag cgattttttt acttcctgaa tttctttttt
    11401 aaatttttca actttgttaa aataccctga tttttcatag tggaggagaa ggtagggatg
    11461 gatttttatt tattgtctct ctgtgccttt gtgctttgaa ttcatttctt atccgccaca
    11521 ctacgagtcg gacactaaaa taagcacctt gaaatttttg gactttgtgc cttaactctt
    11581 tttatctcgc tactttctac tctctgcctt ctacttcaaa gagtacctgt taaaggaatt
    11641 taacttttcc tgtctgtaag taaaaaccag tatgaaaaaa gttctttttc tattttatct
    11701 gaattcttga atttcttttt aattatgtta taaaattctt tattatgttt tctgtaaatt
    11761 ctatgtagta cttcatggaa aacaatatac ctaacaaatc ttgtaggaag aaacatcaat
    11821 aatttattga tagttaaatt cccatcataa ctacaacttc cccattttgt tttcatcttt
    11881 ctaaaaaata ttttatttac attgacagtt aagtcacttg aatattcttt cgcaattttt
    11941 tctacttttt tagtaaaaat tttcttatct acctttgact ttaactctaa cttttttgct
    12001 tcttttaatg tctcttcaat aaaacaaaat tttttaaaaa cccattcctt atgtttttcc
    12061 agcaattgtt cttccccata ataattttca ggtaatatga cagataaatt tcctgttttg
    12121 aattctaaac gaggatactt aactttccta tagacctttt tgtatggtat ttttttatcc
    12181 tttattttta tcgtttccat attttatctt cccttaacca cttcatattt aagtagagag
    12241 aataacctct ttaaaatccc tcttaatctc cctctctttt ccccctcatc cctcgggcgt
    12301 agtcctgagg ggatccgtag ccaaaagcga ggaccctacg ggtgagcata atcttctccc
    12361 cttgggggag aagaaataaa aagaggaatt tcaatggtgg aacaataaaa ttgggcatga
    12421 accccagcgg agagaagaaa taaggaagat cgcatttttc ttttttgttc cccctctctt
    12481 tttatcctct ccccagtggg gagagggtgg ggtgaggggg tctttacttt gtgcctgaaa
    12541 ttaaatctct tccttgggca tactccaaga acgagcataa tcccttcctc caaagtgtgt
    12601 gtcggacagg gttttaaaaa agaagggaat tttattgtgt tgtcctttat tatctctggt
    12661 gtcaaattgg tataagtcat tgattttcat gcacttgtta acttcgtaag ttaacggagg
    12721 gcatcaatgt atttggcgag gatgtctatt tctatgttta ctatgtcccc tgtcttttta
    12781 tatttgaggt ttgtgttttc tattgtatat gggattatgt tcacttcaac tctgtttccc
    12841 tttatttctg atattgttaa agagattcca tctattccaa tacttccctt atttacaaaa
    12901 tattttctgt aattttgagg aatatccaat acaattttgc ctgtttgaga tgattttata
    12961 aatgaagaaa tttttgtttt gaaatcaata tgccctgtta gaaaatggcc actgatttta
    13021 tctcctaata ttaaaggtgg ttcaagattc acaacatccc ttatttttag ttgtcctaaa
    13081 tttgtttcgt tttctgtgaa ttttgttatg gaaacaaaaa ggcatttttc ttttttgccc
    13141 tcaattgtaa gacaaacacc atttactgca atactatcac caatttggag tttttcatat
    13201 aaagggaagc attcaatttc aagttctgta atatcccctt tccctctttt attttttata
    13261 gttcctgttg cctgaattat tcctgaaaac atactttccc ccttattaaa atatcatcat
    13321 caaaaaattc aattttccta ttttctaaat aaaaactttc actaattttt tcaatatctt
    13381 ttccacctat tacatttatt gaattttctc cacctattat cttatttcct ataaatatta
    13441 aaaattcatc aacttttctg ttttcaaaaa accatgtcag gacatttttc ccaccttcta
    13501 ccattataat accaatttct tttttatata attggcccag aaattcatca attttaaatt
    13561 tcccattttc cgtagataga caaacaaatt caaagttttc ttttttctgg tgataaaaag
    13621 atattttttc tttatctact ttatctgaaa taaaaattaa aactttactt ttttcatttt
    13681 tccatatatt actttcaaga ggtgttttta aatgtgggtc aattataatt ttataatatc
    13741 tttttctttc aagtaatttt ttttttactt caaattgagg aggaatataa tcaagaaaag
    13801 ggttatctgc aagaactgta tttattccaa ctacaatccc atctatttca aatctttttt
    13861 ctctgacgaa ttttcttgct ttctcccctg ttatccattt tgaatcacct gtttttgttg
    13921 caattttccc atctattgtt tgagcccatt tcaaaattat gtaaggtctt ttttttgtta
    13981 tatatgttat atatggctgg tttaattgaa aagccccctc aacagggaag agtcggactt
    14041 ttattccatt ttcctgcaag attttaaatc catttccatt tactattgga ttggggtctt
    14101 tcatacagga gataacttct tttattcctg aagatattat tttttctgtg cagggtgggg
    14161 tttttcccca atgacagcat ggttcaagat ttacatataa agttgctcca attgtatttt
    14221 ttcctgcttt ttttattgct tcaacttctg catgtggcat accaaaacac tgatgatacc
    14281 ctgacccaac aattttactt ttctggacaa ttatagcacc caccataggg tttggactta
    14341 caagcccttt ccccttttct gccaatttaa ttgcctgttt taaaagtttt atatggtcaa
    14401 ccatttattt caataacaca ttttagaact tcaccttttc ttactgcttt tattccatct
    14461 gtaatttttt caagtggaag ggtcattgta ataaattttt ttgcatcaat tttcccttct
    14521 gatattaagt ttaaagcagt tatatagtcc tctctatttg aagcaaagga accaaataca
    14581 gaaagttctt tataatgaat tgtgtttgag tcaattgtaa ttcttgaatc atcttttgga
    14641 agtcctccaa aaaaacttat tctacctttt tcagcaagaa gttctattcc atctatctgt
    14701 gcttgttttg aaggacaggc agttatgaca acatccgggc caacattatc agtaatttgc
    14761 ataatttttt ctttaaaatt acttttccca tccagtaaaa ttatatcagg aaaactctta
    14821 ccgaattttt caagtcgttc ataagcaggg tcaacaatta ttactttact acctccttgt
    14881 gcttttgcta aaacagagtg cataaaacca ataggtccac ctccaaaaat aacaacatta
    14941 tctccatcat tgatatttaa gtatttttgg ccatttatac aacaggaaag tggttcaatc
    15001 aaagtccctt caataagaga aacattatca ggtaatttta aaattacttt ctgttttact
    15061 gcttcttctg gaacaacaag gtattcagca tatccaccct gataataata cccaattgct
    15121 tttgtatctg gacataaatt ataatatccc ttctgacaca ttttacattt tttacatcca
    15181 attgatgtta caagggttat cctatctcca actttaattt ctggataatt aacatttcta
    15241 ccgatttcta ctatttcacc tgttatttca tgaccaatta ttgcaggtgg atttactttt
    15301 ttatgtccag agaagtaaat tctcacatca gttccacata ttgcacaata gtgaactttt
    15361 attttaactt cattttcatt acaagttggt tctttaaagt cctctattct aatgtcttca
    15421 ataccataaa aaacagccgc tttcattttt tcctccctcc ttattttctg gtactgccca
    15481 aattgtctaa tcaaattcac tccacacttt ctcacaaaga caaatgaaat gtgaaaagta
    15541 agatgtgaga agtggaaatc tccctcaatc cccgttgtgc ctattgaaat acattacggc
    15601 accaactctg cttgtaaatc tttgctacct cagtcggttt gttaaagggg ggaacttata
    15661 ctccttccct ttccaaggga aggatgggat ggatttttat taaattccct cttaaaatcc
    15721 ccctcaatcc ccctttttca aagagggaaa ttacttactc ccttttattt tatcctttta
    15781 ttacagcaat aggtatcatt tttgcgactt tttctgatat tcctgctcct tcaacaactt
    15841 ttattacttc atctacatct ttatatgctt ctggcatttc ctctgcaagt ccttttctac
    15901 tcactgattt cgctattatg ccttttttac tgagttcaac atcaatttct ctcccttttg
    15961 cctgttttat tgcctggtgt cttgataatg ttcttcctgc tccatggcag gtgctaccaa
    16021 atgtttcttc aagtgctttt tcagttccta ccaatatata tgaagcagtt cccattgttc
    16081 ctggaattat aactggttgt ccaacatctt tatatttttc cggtaaagaa gggtgtcctt
    16141 ttgggaatgc tcttgttgcc ccttttctat gaataagtag ttttttcatt cttccatcaa
    16201 cattatgtgt ttccagttta caaatattat gagcaacatc ataaacaaca aataattctt
    16261 gcttttccca tggctgtttt aaaattcttg aaaatgtctc tcttatccag tgagttatta
    16321 cctgtctatt tgcaaatgca taattagctg ctgccttcat agcagaaaag tatcttttcc
    16381 cttctggtga actaaaagga gcacaggcaa gttgtctgtc tggaagtttt atcccatatt
    16441 taagaacgac tttcccaaat ttatctaaat aatcatcaca tatctggtat ccaaagccac
    16501 gacttcctgt atgaaccata attgtaatct gtccttcaaa tattccaaac ttttctgcta
    16561 tttccttgtt ataaatattt acaacctgtt gtatttcaag aaaatgattt ccagaaccta
    16621 aggttcctga ttgtggtaaa cccctttcgt atgctctatt acttatgact gatgggtccg
    16681 ctccttccat acacccatat tcttccatat tttccaaatc atcttctatt ccataaccat
    16741 tttttactgc ccatttcgct ccttcaacaa taactttctc aagttctttc ctttttagtt
    16801 ttaattttcc tgttgaacca acaccagaag gaacattata aaaaaggcca tttgccaatt
    16861 cagaaagttt atcttctatg tcatctcttg taagattact ccttataagt cgtactccac
    16921 aatttgaaac aagaaagtta ttaccaacaa aattatgaga tttatggttt attgttaaat
    16981 cataaacata cccttcatat tgtattttct tgatttcaac gattttatca aatacaaaac
    17041 cagattctcc attttttgtt cttacttcat ttaaaatttc aaaaatacca ttgaaagaaa
    17101 aagaaattct tggttctgtt tttcttcctt cataaactga cctttcaata aatctcctat
    17161 ttacccattc agattgtgtt tttgaataaa tagactcaaa actatatcct gcatttttta
    17221 gacatattgc aactttttct gcttttcccc tttcttttag tatatttttt ttcttcctta
    17281 aataaaaagt gaaaatattc ccaaaaaatt ctttttctct gttgtattca aaccctattt
    17341 tttcataaag atttaacaaa ttttctaatt ttgaagaaag aattaatctt atacggaatt
    17401 ttgtaatgtt attttctcct gtttttattt cttttacact gatttttttt gtatctacac
    17461 caaattcttc aagaatttta gcaatttgtt tgaaaaattt tattccactt tctaaatatt
    17521 gtttttcttt attcattgaa acaataggac aataaaaatt aaacttatta tttttcaacc
    17581 attttggaga actcatttct gctccaaaat aagaagatag aaataatctc ttacaccata
    17641 aaggtaattt aaatatccaa tcaggtatat aaaagtttga aaatgtcttt ttgccataag
    17701 gaacacctaa tgatattaaa aataatgcca atgaccttga agatgtctgg cattcatatg
    17761 tagttgtttc aaattcataa gtagaatatt ctgtttttat tttatgtttc cttttcctgc
    17821 aattcataaa gggtaaaaat ccaagttttt ctatatcctc ttttatttct tttatatctt
    17881 cttggctttt acctgaaaat gaaataagaa aaccctgtcc atcttttaag aaagaaatgc
    17941 tgccatcgcc taaaatatat ccaataattt ttaatatttc aggtaaatgt ggagaattat
    18001 atttcaaagg taaaagttct cttttttcta attgatttat tatttgagat aaagaattgc
    18061 cattggtgtt tttattgaat tgatatgcaa cttctttaaa tttttcaata tcaagaattg
    18121 tttcatcaga catttcctgg tatggaacac ccttaaaagg aaaaatcatt actgaatcac
    18181 caattttcag gtctttaacc tcaattttcc cttcttctgt ccagaaagga tgctcttttg
    18241 ttgctttaat tttataacct gtttcagttt taatttcata aatatcatca taaatttttc
    18301 ttttgataaa atttattatc tcgccttttt ctttttttgt tttttcttta ttaaaacaga
    18361 caatttctcc ccatttttta tcacattttt caattggtaa ataataacca tatttatgca
    18421 atatctttgt ctctccaaca agacagttga tatcgtaccc cacaaatcca ggggcaatta
    18481 caccactttc tatatcaaaa gcaccaacac caccaattgg agcgccgtat ccccagtgaa
    18541 catcgggcat tgctaaacta tattttacaa ttccaggaag ttttgcaaca tttattacct
    18601 gttccactgc tttatcttca attgcttttt gcagaagttt ctcagaaata aaaataactc
    18661 catcaactct cattccatct gtttttggta ttatccattt atattcactt atttttttta
    18721 tattttccat ttttctattg catattcaaa tccaatttca attgcctcct tacttaattt
    18781 tctcattttc tgttctttta taactttgtc aactccataa aacatatctt cttttttaaa
    18841 ttcattgaag aactttttta taaatggaat aacaagacct gcaaaaaaca tattgcttac
    18901 tttactattc ccaattttct ctgcctgttc agttgcagga aattgatata cactaccttt
    18961 attgttgttt ttaataagtg aactattatt tataattatt ccttcatttt ttagatatga
    19021 agaaagtata ttaaatgatg tttcctccat aaaaatacct acatcacatt cagaagaaat
    19081 tggggataaa atattttctg catttgaaat tgttaagata caatgaacat aaccacctct
    19141 catttctgca ccatatacag gaaagcaaga aacatccagg tctctattta tacacgcata
    19201 ggatataact ttccctaagg ttaaaatacc ctgtccccct gtcccaccaa taactattct
    19261 catttttttg tcccttttta ttttgcactt ttaatcaatt cataagtttt ctctatattt
    19321 tctaaatttt catatatttt cttcactggt tttaattctg ttacagaata ataataaatc
    19381 atatcttgta ttaaatcctc caaaagattg attgaaaata aagcatatct tgtttcatct
    19441 actggagatg cctttattga tgatttataa gaaccatcgt tttgataaga ttctttaatc
    19501 atcagcatta attctctgac ttttttgatt agcaaaataa gttcattggg gtgtaattca
    19561 gttttagaat aaaggaaatc aatacaataa acaatcttag aaatatcttc aaggagaata
    19621 tttttattta cataacttaa aaggtataca aaccacagat ttatattttg tggaatctta
    19681 tttagattat ccagaataaa acatactttg aaaaaacttt caaggtcttt tggaataatt
    19741 tttacaatgt tattttcatc aattacactt tgaagtaaaa ttgaaagttt ttctctattt
    19801 atatctatta attcatgttg aaaattaaga atgaaatttt taatatctat tctttgacaa
    19861 acatttttta aagcagtatc tgatttaagt aatttttcaa atttttcttt tggtactcta
    19921 aaaccataac ttcgccatgc ttcaaccatt tcacgttcac catttttaat aattaactca
    19981 ggcttaaaaa ttgcatattt tagtaatggt ctatgtatat acacgaacaa ttttccttca
    20041 atgggtttgt attggtcccc tttctttttt gcaacttttg agatcttgaa atcataaaac
    20101 tttaaatttg acttctcggt atattgaaac tcaaatccta ttttcccatc atttatctct
    20161 gcaataaaat cagggtctct tgaaatcccg ccttttctta aaaaccacaa aaatttatct
    20221 ccacctgatt tataaacttt aacttgtttt ctaggaaaca tttcttttac tttattttct
    20281 aaccactcta atatttccaa ttcagcaatt gcttttttct tcatatcttt acgatattct
    20341 aaataagtgc gagattttat atcgctggat tgggcatata ttgttttccc atctaatttc
    20401 atttttttac ctcaccaaag ttttggttga taattcttta aacatattat tgcatcattt
    20461 gcaatttctg gcactatttc gtatccaatt ccatttcgct ttaaatctgc agatgctaaa
    20521 agcgtagttc cagaaccaac aaacgggtca agaactgttt ctccaatata actgaatgct
    20581 ttaattaact tgtaaggaag ttcataagga aagggagcaa tatgattgcg tctatcacca
    20641 ctaccctgag gtttcatcac ccagacatca ctttttttta tagatagcca gaattctttg
    20701 tctaattttg attgctcttt ttgttcctta cttaaatgac catatttatt aaatcctttt
    20761 ttctcaggtt tatcaaattc aagaataaat tcgtgcatat tatttatcaa aattccaccc
    20821 gggtatgggt atgttccaaa atgagcacga accgcatttg ttttatgcca tataatatcc
    20881 cgtttaaaaa taaatccaat tttttcacat atatctattg ttctcccaac taaatttaaa
    20941 gttttaaaac taccatttcc aagtcgtaaa gggagattca taatgtttat aaatgctttt
    21001 cttcctggtt gtaatactct ataaacttct ttccatacta tacttatttt gtcaaaccac
    21061 tcatcaatat catgaatatt gccaaggtca ttttctatag gttttcttga atacattttt
    21121 gtatcaaaat atggaggcga agttatcatt agatgagtac tattatcagg taaatcactc
    21181 attaacattg aattttttac aaatattttt tgaattgtcc catttatttc tatgatattg
    21241 acatcattaa ctttaatttt atttgtatgg aatttatttt taaatttttc ttcatacttt
    21301 tttagttctt ttaaatcaaa tcgcatt
//
LOCUS       DAOOVW010000029        20717 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_1738_length_20717_cov_7.152841, whole genome shotgun sequence.
ACCESSION   DAOOVW010000029 DAOOVW010000000
VERSION     DAOOVW010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 20717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20717
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1738_length_20717_cov_7.152841"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..139
                     /locus_tag="PLW95_05265"
     CDS             <1..139
                     /locus_tag="PLW95_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HOV22072.1"
                     /translation="IKFPLGKLPGDIIIKKENFVFYFPITTGILISIILTLIFSIIWR
                     K"
     gene            139..1509
                     /locus_tag="PLW95_05270"
     CDS             139..1509
                     /locus_tag="PLW95_05270"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOV22073.1"
                     /translation="MERKNGWEVFSTEEKNNVFKFCDEYKEFISRNKIERKLVNECVE
                     TAEKNGFKEINKYKNLKKGDKFYKKNKEKEVIFGIIGKEILKKGAKFIVSHIDSPRLD
                     LKTSPIYEDENICLLKTYYYGGIKKYQWLTIPLALYGIVILKTGEKVEIEIGEKENES
                     IFTITDLLPHLSKDQYKKTIEEGFPGENLNVLFGTIPLTEKTEEKEKVKKNVIKILKE
                     KYGIEESDFISSELHFVPAGKGRDAGIDKSMILSYGQDDRVCSYTSFRAINEIENPIK
                     SVFCILVDQEEIGSYGTTSAQSSFFRYLIEEVVEKTGGTLSDIRELFENSKAISADVD
                     SLLDPNFKEVSDPRNTARINCGVVLNKTTGGRGKGGSIEPSAEYIGYIRKIFDQNNVL
                     WQTGEIGKIEQGGGGTVATYFARYNIDTIDCGTGVLSMHAPFELTGKIDIYSTYRAYL
                     SFYKED"
     gene            1512..3146
                     /locus_tag="PLW95_05275"
     CDS             1512..3146
                     /locus_tag="PLW95_05275"
                     /inference="COORDINATES: protein motif:HMM:NF039643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6067 family protein"
                     /protein_id="HOV22074.1"
                     /translation="MALSDLKIWIPNPLEKIFKEDLPKKEQQVKKEIFLFSAKNEYES
                     FQIALRSSVMLKNVRVEFSDLLNVSGSKISKENLKYNFVDYVPVNIDNSGLKEVVRIS
                     PGEFPDPLLEDKSIDIPPQETKTIWFTIYVPPDVEEGKYKGEVKLLTNKGTFSIDLNL
                     KVWNFILPNESHMYLTNWIMSPSALSKFHRVKIWSSEFWNLVEMYAKNMKEHRQNVII
                     THLFELINFFTDERGNLIFDYQNFDKWVNIFLNNGVKLIEGSHLAGRVGTWNDPNLYS
                     PLIKVRTKEGKIIFHSRILVGHSDFYKYIGNFLMSLRNHLKEKGWIDKFIIHLSDEPD
                     SICAPSYKLLSKIVKDFFPEVRRIDAATIPDIVGSIDVWVPLLSGNFSSFFLERQNFD
                     EEVWWYTCCGPRDDKYPNRFIDYPLIKVRIIHWLNYKYNVKGYLHWGYNYYKDWTGSE
                     FIDPWKENTAKMWPPGDPFIVYPGKNNKILNSIRWEQIREGIEDYEYLYLLDKKNPKV
                     LQKILSSILPNLTDYTRNPVDLLNMRQKIGEEIEKK"
     gene            3160..3549
                     /locus_tag="PLW95_05280"
     CDS             3160..3549
                     /locus_tag="PLW95_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOV22075.1"
                     /translation="MEKISVLTNKRNEFLEITSKIEEKVKKSNVKNGICFIYIPHTTC
                     GLTINENADPSVKNDILNKLEEIVPENSGYTHTEGNSDAHIKSSILGHSLFVFIENGS
                     LKLGTWQGIFLCEFDGPRTRQVWIKIM"
     gene            3561..4595
                     /locus_tag="PLW95_05285"
     CDS             3561..4595
                     /locus_tag="PLW95_05285"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOV22076.1"
                     /translation="MEITGKNLNEIIELSNLVFRPKEKSMKIETPLMWEKGKHFVIKE
                     NEEIVSFIGMVEEEINVFGYKMKIGNIGNVCTHPDYRNKGYATCLLNETIKKAKEDKL
                     VYLMISGGRELYKRAGAVSMPFYFYQFSGNKGKTDFRVRRYKDDLYCECIKIYQKEPV
                     RFLRENKGFSSLFSLYNGKFKKKVMHMGSKIWIISKNKEILAYWAEGETKDGISIGYN
                     REYGGIRELLAKVWKSRGKKVNIYIPLWDEELKYYLGKEKEVKEMGTILLINPNLLIE
                     NLSGYFIERGVEIDYLKRENKILLKANKKEKEFKDNKDLARFLLHLNNEEVWKNIFPI
                     PLPFYGFNYA"
     gene            4605..5009
                     /locus_tag="PLW95_05290"
                     /pseudo
     CDS             4605..5009
                     /locus_tag="PLW95_05290"
                     /inference="COORDINATES: protein motif:HMM:NF013798.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
     gene            5142..5549
                     /locus_tag="PLW95_05295"
     CDS             5142..5549
                     /locus_tag="PLW95_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOV22077.1"
                     /translation="MTKKTYKNPVPTVDIIIEYKKDIILIERKNYPYGWALPGGFVNY
                     GESLEKAAEREAKEETGLELEDLKQFHTYSQPGRDPRYHTISTVFTAKGKGKLKSGDD
                     AKKVKIFNKENLPENVAFDHKTILSDYFECISS"
     gene            5592..6584
                     /gene="trpS"
                     /locus_tag="PLW95_05300"
     CDS             5592..6584
                     /gene="trpS"
                     /locus_tag="PLW95_05300"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOV22078.1"
                     /translation="MSEKSILSGMRPTGPLHIGHLFGALKNWKKLQDDGYKCFYMIAD
                     YHALSTEYANPQKIKENVEEVAIDFLISGLNPEKSTIFIQSLVPQHSELHLLFSMFVP
                     IPYLERNPVYKEQIKELKDKDLHTYGFLGYPILQAADILLYKATYVPIGVDQLPHLEL
                     TREIARRFNYLYGETFPIPEALLTETPKILGTDGRKMSKSYNNCIYLKDSPDIIKKRV
                     STMFTDPKRIYRKDKGHPGKCPVFMYQNIFNKERVGEIKKSCKEGEIGCTDCKKELGE
                     KVVDFLKDIQEKREELSKNKEYIWKILKNGSEKAREVATKTLKDVKIKIGVNYE"
     gene            6577..7680
                     /gene="hisC"
                     /locus_tag="PLW95_05305"
     CDS             6577..7680
                     /gene="hisC"
                     /locus_tag="PLW95_05305"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOV22079.1"
                     /translation="MNKNIEKLVKEGIKKISPYEPGKPIEELQREYKVKKTIKLASNE
                     NPFGPSKKVKKAIEKSIENINRYPDGSSYELKNELSKYLKLPVENIMIGSGSSEVISL
                     TLETFINPGEEILYPWPSFTMYRIFALKNNAIGVEIQLENDFSYNLTKFLERITPKTK
                     VIILCNPNNPTGTIIKKREMENFIKNLPDDIILISDEAYFDYVESNDFGSAFPFFKEK
                     NIIICRTFAKIYGLAGLRIGYGIANKDIIGYIERIRPPFNVTTPSQMAAIASLDDQKY
                     IENVKKKTIKGKKYLYSEFEKMGVEYTHSESNFILCKFGKNTNKIVKELEKRGIIVRG
                     MASFGLPEYIRITIGTEEENKIFINNLKDILKK"
     gene            7707..8438
                     /locus_tag="PLW95_05310"
     CDS             7707..8438
                     /locus_tag="PLW95_05310"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015219946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOV22080.1"
                     /translation="MIKLGINIDHVATLRQLRREVFPDPVGAAVICELAGCDSIVCHL
                     REDRRHIQERDLKLLKEVVKTKLNLEMALSEDVIKIALETKPDQVTIVPEKREELTTE
                     GGLDVITNFEKIKKVVETFHKSGIKVSIFIEPDLNQIEKVKETGANFIEIHTGKYSIV
                     KTEDEIYNELNKIIKGTEFALSIGLRVNAGHGLDYKNVTPICKINGIEELNIGYSIIC
                     MSVFTGLHKAVTKMIKTIRENERKD"
     gene            8422..9090
                     /locus_tag="PLW95_05315"
     CDS             8422..9090
                     /locus_tag="PLW95_05315"
                     /inference="COORDINATES: protein motif:HMM:NF024595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HOV22081.1"
                     /translation="MKEKIKECFKEPVRGKINLFNLLIGGVLTFFPIVNLIPLGYLTE
                     KLRRKLENKEKVVGWENLWYLMKSGWNTFILILGYFCIAIFFVFLGVIFIFPLSRGKI
                     MSIFFFRGIVLINIGTIIFLLGLFFLPFSIPVYLETKSLKKAFDIREVLSRILLKLND
                     YLVLYITIIGVIVFSFVITFLLLNWVMGLLLGGFLYFYAGSVIINLISKFFPKKYFTV
                     QLPF"
     gene            complement(9402..10124)
                     /gene="tatC"
                     /locus_tag="PLW95_05320"
     CDS             complement(9402..10124)
                     /gene="tatC"
                     /locus_tag="PLW95_05320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOV22082.1"
                     /translation="MKTQKKNQNEYSFIEHLEELRRRIIWIIVIWVLISVIAYFFSSQ
                     ILDFLIIPLKKYQKNVIFTRPLEPFFSILKICVFIGGIISIPHIIIQLYLFVSPALTE
                     KERKISKVISIFFPIFFFSGVIFTFYFIIPLGIKVLFSFGKGVLTPFVSIGYYLNFLL
                     IFMILLGLVFNLPVFFSALAAIGLVKSNFLKQKRKYAIVGAFILSAIITPTTDIITQT
                     FVAILLIFLYEISIIFVRIFET"
     gene            complement(10096..10281)
                     /locus_tag="PLW95_05325"
     CDS             complement(10096..10281)
                     /locus_tag="PLW95_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012474046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOV22083.1"
                     /translation="MFNIGAGELILILLIILLLFGAKRLPEIGRSIGKALGEFKKGLK
                     ETKEEIEKDEDTEKKSK"
     gene            complement(10291..10866)
                     /locus_tag="PLW95_05330"
     CDS             complement(10291..10866)
                     /locus_tag="PLW95_05330"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOV22084.1"
                     /translation="MKRREFLKNFLFISSIFLVSGRYKFKGKNTYSKKSIIRPPGAIE
                     EDEFIYKCIRCGECFKVCPSNCLFSVPINKDIFSFGTPEIIPRKGGCIMCLACGDVCP
                     SQAIKKIDEKLKIGTAEIDKKRCLVWTEEKDCFVCLEYCPEGAIFLDFKRRPYVNYEK
                     CIGCGLCEENCPVIGESAISISTKGAVRLKI"
     gene            complement(10844..11476)
                     /locus_tag="PLW95_05335"
     CDS             complement(10844..11476)
                     /locus_tag="PLW95_05335"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOV22085.1"
                     /translation="MFKKYLRRLCQLIFIFIFFYLLIRNEYLIKNVIPVNLFFRLDAL
                     LAIFTTISTCHFTYYFFPSLVILFLLILFGNFFCWWICPFGGIIDILNMVFLRKKWKF
                     LRRRPIFLKKLNIFLFILIIFTAILVNFVKIPNFFWFFDPFVIITRAFVFKNWYLFVF
                     LFILLLSILWNRLWCLNICPLGFFYSLIGVKLRNFLIKKRRRKIEKERVS"
     gene            complement(11469..12356)
                     /locus_tag="PLW95_05340"
     CDS             complement(11469..12356)
                     /locus_tag="PLW95_05340"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOV22086.1"
                     /translation="MERREFLKRTFLAIAGFEISRHLKFGKLSYAQSKGSEIYVAKGG
                     NPEENTYRAIKGVGGIEKFVKRGSKVVIKPNIAWNRTPEQAATTNPDVVLGIVKLCKK
                     AGASEVIVIDNPCNPWQVTYKISGIAEVVEKGGGIMKPPIKFKQVNIEGTKILKNAEI
                     LEEVLDSDVFINVPIVKVHGGSKVTIGMKNLMGIVKDRGFFHRTDLHRCIAEITYYIK
                     PSLTIMDATKILLTMGPQGPGVVKDIGIVAAGTDIVSLDAYGTKLLGQDPYNIPHIKI
                     AEEMGLGIADLNKVNIKNV"
     gene            complement(12631..13251)
                     /locus_tag="PLW95_05345"
     CDS             complement(12631..13251)
                     /locus_tag="PLW95_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016646897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOV22087.1"
                     /translation="MNEFNAFLVRSIGGWGYFGIFVLMFLESSFFPFPSEVIMIPAGY
                     LSYLGKLSLWVSILFGVIGSYAGALFNYFLAEKYGRKFLIKYGKYLFIDEKKLGKLEG
                     FFKKHGHISTFNGRLIPGVRQYISLPAGLAKMNLFIFSVYTIVGAGIWVSILTFLGYF
                     IGDKKDLIKKYSNEITILIIIFVVIITLVYWIYYKKKNHIKGEINH"
     gene            13354..13458
                     /locus_tag="PLW95_05350"
     CDS             13354..13458
                     /locus_tag="PLW95_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOV22088.1"
                     /translation="MRKEAKRWLKEAEFDIKAAKDNLKEGNFNIYLFY"
     gene            complement(14082..16022)
                     /locus_tag="PLW95_05355"
     CDS             complement(14082..16022)
                     /locus_tag="PLW95_05355"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOV22089.1"
                     /translation="MKKSCNKKLLFFSVCGLFLSIFCKSMYGDNQLILVENGQAKAGI
                     LLSGNPSAKESRSAKIIKEHIEKITDTEISMLKISDLKNPRYSLSGIEVNGELKNFNF
                     ILIGSYDFLDKLGISFEGLGPEGMIIKTAGNVVVISGNDEQGLRHATYTFLEELGCRY
                     LWPGELGKVIPKKNTLYISKLDIKESPRLWTRNIRVTSPKSSRILDGLKKLNFDGEKY
                     KNILTKTTQSEETDDGWLAWHRMGGHKQVYRAHSYGDYWEKYGKDHPDWFALQPNGIR
                     DQSLSPDRCRLCLSNRELINEIIKNKIQELGKKPTQDGVAIGLNDGGRTTFCMCENCR
                     KLDPPEGRPITLYDYTTGNKIPFQYVSLTDRVLWFSNQIAEGVSKVFPDKHLGYDAYS
                     AYYAPPINVKPHPNLIICFVNGSYTDDEARKQILKDWDEWSKYGNKMFYRPNSLLANR
                     DNIVPQNFSHKIFEDMKYLYDSGMIGTDFDSCEQHWALMGLTYYVLAKSHWNPDKINI
                     DSLIDDYCEKGFEGASEYIKKYFSLIENVCNEAAEKKINILEVYSDDIFQKLNSYLEK
                     AENISKNIENKKVFERVEFLKVGLKFGELQVKKYNISKQLKEKADPEIKEKYNNIQKE
                     YLDLLKKIIEENPTAVNVPIVA"
     gene            complement(16019..16810)
                     /locus_tag="PLW95_05360"
     CDS             complement(16019..16810)
                     /locus_tag="PLW95_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22090.1"
                     /translation="MKKVIILFIFLSIFLVALADEVKKPVAILGYGGPITAVEYRIFK
                     PESIEYKVFDDWLQPSEYYNFSVIYMAERIGQLPTVKENKGNMWTDPEVLKKVEEYLK
                     SGGVIIITCCAFDGLAEGRNLGAISHLLGFGYYPRYPRGKSPYKVKIVNKKSPITAHL
                     KKEEYDWIADTTPISNVSSAEVLAVQVDENGEVLSPFITENIIGKGKVYWFGTPLFRL
                     YQKKQLDPEVEEYRNTLFQAILQGNPARGEIKKEKWEPIPLGGKK"
     gene            complement(16848..20105)
                     /locus_tag="PLW95_05365"
     CDS             complement(16848..20105)
                     /locus_tag="PLW95_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22091.1"
                     /translation="MRKRNFKLFLVLSFISFLFTAKGGIILKEDIFQPTGQKSKVIET
                     PYYKVTIVPDAGGRILSLIDKETNTELLHSVISNNCSLWGGLLDDKGGRENKPYVFKI
                     LSSNKNEIKISLSYKDEEKQQSVIKELTFYDKVPIIGVSYTYINESQKKMTYNDTAVR
                     NGILPGGEVVSDEDIYCVPTIKGVRRFSPTKSPGQMPEFSQKIKKQVGAPWHAFLNKK
                     KLTGLTFVHLDDGYTGWYMWKGGIPRPTYEWFYKSVPAGKKVTYKLLIILTHGFETLI
                     DAKPDYLLDIRDISQTEKELMLEIKISSIWKSLSNVKIILHLEDFNGNIVKNIPEISF
                     KNVVVDKPQTKYITTGCPEKGFYVLKGEVITTGKTKWQFEDILSLRAGHFVDGPYERK
                     IQYLSISEYQDISGWKKEEEKEISQPSEEDKKRGYMVYSEIGEEAGKLLKGYNLDLVK
                     GEFESFRIVYYPLKEDIGMVSWGATNLRDIKTGFPVEIYPKMRIEEVEEFDASIWGYE
                     DPFILHKLVSNKRFEGNKKQYLWLTFSTDKLEPGDYIFDIIISPDKCSTLKIPVNIKI
                     RQISMPDQLVSLEVEGYIGSFGHPEENLRGLKSYLCDLGQHNADFLQADRRWILNNAR
                     ARDSGQLFLNYEKLYKNVDEKELENKFLDFSYYNGWIYEGIKQGLIRFKTEYYSTEVP
                     QGFTKWALKELYWYVKERGYPKNSIFIKNLDEQPTDIYPKMAEVGKVLQEIGWQPFTT
                     VDALLGQPEELKVLSPYFRMYQCGSFSEPDVVEKWVKEGILRPTDEIWEYIGSGTSSL
                     DYQYFISKGWYIGQHNLAGFHNHEYLRGGGERLGALIIFPTPVGPLSSPAWEGLRDGI
                     DNANLYRYAQHLIKAVEEIKLVETTQLIKWQEQLSKIVSEDEDAIIHMVRQPIKGGLE
                     RTDIKDFTPEQGIEARKVLLDLLEDILEKTGKKLKYDIRWGEVKLVDKGELVYFPSIG
                     KGISSADMFPLIDKLSQGTLNEIKAEPLEKVVKETSVIVAGTLADPIIQQIINKEDYL
                     KERYTNSYPKQGDYAVIETNNPFADGKRIIIILGGDDRGVLLGIKQFSYFLKKIL"
     gene            complement(20126..>20717)
                     /locus_tag="PLW95_05370"
     CDS             complement(20126..>20717)
                     /locus_tag="PLW95_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOV22092.1"
                     /translation="ELLVVIAIIAILAAMLLPALSNAREKARQASCMNNLKQIGLGFF
                     MYAQDYDGWCPAYRPFGSPYSSWDKYIDPYIPLKRYDIKRVWICPSHTKWTGGGTGWM
                     TYAMNSYLPGFFWKLDRMKHSTTGFLISDVTKNSSGTYRITPDAYPDEMGFHHMGYAN
                     ILFLDGHVAPKRVEDIPKWADRYKSYWNAFWRPWLD"
BASE COUNT         7563 a         3126 c         2868 g         7160 t
ORIGIN      
        1 aataaaattc ccacttggaa aattacccgg tgatataata ataaaaaaag aaaattttgt
       61 attttatttc ccaattacaa ctggtatttt aattagcata attttaacct taatattttc
      121 aataatatgg aggaaataat ggaaaggaag aatgggtggg aagttttctc tacagaagaa
      181 aaaaataatg tttttaaatt ctgtgatgaa tataaagagt ttatttcaag aaataaaatt
      241 gagagaaaac ttgtaaatga gtgtgttgaa actgcagaaa aaaatgggtt taaagaaata
      301 aataaatata aaaacttaaa aaaaggagat aaattttata aaaaaaacaa agaaaaagaa
      361 gtaattttcg gtataatcgg taaagaaatt ttaaaaaaag gggcaaaatt tattgtctct
      421 catatagatt ctccacgact tgacctaaaa acatctccta tttatgaaga tgaaaatatt
      481 tgtcttttaa aaacctacta ttatggtgga ataaaaaaat atcaatggtt aacaattcct
      541 cttgctcttt atggtatagt tattttaaaa acaggtgaaa aagttgaaat tgaaatagga
      601 gaaaaggaaa atgaaagtat ttttacaata accgaccttc ttccacatct ttcaaaagac
      661 cagtataaga aaacaattga agagggtttc ccaggagaaa acttaaatgt gttatttgga
      721 acaattccat tgacagaaaa aacagaagaa aaggaaaaag ttaagaaaaa tgtgataaaa
      781 attttaaaag aaaaatatgg aattgaagaa agtgatttta tcagcagcga acttcatttt
      841 gttcctgctg gaaaaggaag agatgcggga atagataaaa gcatgatatt atcttatgga
      901 caggatgata gagtttgttc ctatacatct tttagagcaa taaatgaaat agaaaaccct
      961 ataaaaagtg tgttttgtat tttggttgac caggaagaaa tcggtagtta tggaacaact
     1021 tcagcacaat catcattttt tagatattta attgaagaag tagttgaaaa aacaggtggc
     1081 acgctttctg atataagaga actatttgaa aattcaaaag caatctctgc tgatgttgat
     1141 tctttattgg acccaaactt taaagaggtc tcagacccaa gaaatacagc aagaattaat
     1201 tgcggtgttg ttttaaacaa aacaacagga ggaagaggga agggagggag tattgaacca
     1261 tcagcagaat atattggata tataagaaaa atttttgacc agaataatgt tttatggcag
     1321 acaggtgaaa tagggaaaat agaacagggg gggggaggaa cagtagcgac ttattttgca
     1381 agatataata ttgatacaat tgattgtgga acaggtgttt tatcaatgca tgcacctttt
     1441 gaattaacag gaaaaattga tatctactct acttacaggg cttatttatc attttataag
     1501 gaggactaaa aatggcactt tcagatttaa aaatttggat tccaaatcct ttagaaaaaa
     1561 tttttaagga agatttacct aaaaaagaac aacaggtaaa aaaagagatt tttttatttt
     1621 ctgcaaaaaa tgaatatgaa tcttttcaaa ttgctctgag aagttctgtt atgttgaaaa
     1681 atgtaagagt tgagtttagt gatttactga atgtatcggg tagtaaaatc tcaaaagaaa
     1741 acttaaaata taattttgta gattatgtcc cagtgaatat agataattct ggattaaaag
     1801 aagtagttcg tatttctcct ggagagtttc cagatcctct attagaggat aagagtatag
     1861 atattccacc acaagaaacc aaaactattt ggttcacaat ttatgttcct ccagatgtag
     1921 aagaagggaa gtataaaggg gaagtaaaat tacttactaa taaaggtact ttttctatag
     1981 atttaaattt aaaagtgtgg aattttattt tacctaatga gtcccatatg tatttaacaa
     2041 attggataat gtccccttct gcattatcta aatttcatag agttaaaatt tggtcttcag
     2101 aattctggaa tttggtagaa atgtatgcga aaaatatgaa agaacatcgc caaaatgtta
     2161 ttataactca tttatttgaa ctaattaatt tttttacaga tgaaagagga aatttaattt
     2221 ttgattatca aaactttgac aaatgggtaa acatattttt aaacaatgga gtaaaattaa
     2281 tagaaggttc tcatttagca ggaagagtag gaacatggaa tgacccaaat ctttattctc
     2341 ctcttattaa agtaagaaca aaagaaggaa agattatttt tcactccaga attttggtag
     2401 ggcattcaga tttttataaa tatataggaa atttcttaat gtccttaaga aatcacctaa
     2461 aagaaaaagg atggattgat aaattcatta ttcatttatc tgacgaacct gatagtatat
     2521 gtgcaccttc ttataaactt ttaagcaaaa tagtaaaaga ttttttccca gaggtaagaa
     2581 gaattgatgc tgctactata cctgatattg ttggttcaat agatgtatgg gtgcctctat
     2641 tatctggaaa cttttcctct ttctttttag agcggcaaaa ttttgatgaa gaagtttggt
     2701 ggtatacctg ttgtggtcca agagatgata aatatcccaa taggtttata gattatccat
     2761 taataaaagt taggattatc cattggttaa attataaata taatgtaaaa ggataccttc
     2821 actggggata caattactat aaagattgga caggaagtga atttatagat ccatggaaag
     2881 aaaatactgc taaaatgtgg ccacctggag acccttttat tgtatatcct ggaaaaaata
     2941 ataaaatttt aaattccatt cgctgggaac aaattagaga aggaatagag gactatgaat
     3001 atctctattt gttagataag aaaaacccaa aagttttaca gaaaatactt tccagtatat
     3061 taccaaatct tactgattat acaagaaatc cagttgattt actaaatatg agacaaaaaa
     3121 taggtgaaga gatagaaaag aaataatttt tataataaaa tggaaaaaat ttctgtatta
     3181 actaataaaa gaaatgaatt tttagaaata acttcaaaaa tagaagaaaa agtgaaaaaa
     3241 agtaatgtaa aaaatggaat ttgttttata tatattccac atacaacctg tggtctaaca
     3301 ataaatgaaa atgctgaccc ttcagtaaaa aatgatattt taaataaatt agaagaaatt
     3361 gttcctgaaa attctggata tactcatact gaaggcaatt cagatgccca tataaaatca
     3421 agtattcttg gacattctct ttttgttttc atagaaaatg gttcattaaa attgggtacc
     3481 tggcagggaa ttttcttatg tgaatttgat ggaccgcgaa caaggcaggt ctggataaaa
     3541 attatgtagg agcaaaataa atggaaatta caggtaaaaa cttgaatgaa attattgagt
     3601 tatcaaattt agttttccga ccgaaagaaa agagtatgaa aatagaaaca cctctaatgt
     3661 gggaaaaagg aaaacacttt gttattaaag aaaatgaaga aatagtttcg tttattggaa
     3721 tggtagaaga agaaataaat gtttttggat ataaaatgaa aattggtaat atcgggaatg
     3781 tatgtaccca tccagattac agaaataaag gttatgctac ctgtctttta aatgaaacaa
     3841 ttaaaaaagc aaaagaagat aaattggtat atcttatgat ttcaggtgga agggaattat
     3901 ataaaagagc aggggcggtc agtatgcctt tttattttta tcaattttct ggtaataagg
     3961 gaaaaacaga ttttagagta agaagatata aagatgattt gtattgtgaa tgtattaaaa
     4021 tttatcaaaa agagcctgtt aggtttttga gagaaaataa aggattttca tcactttttt
     4081 ctttgtataa cggaaaattt aagaagaaag taatgcatat gggaagtaaa atctggataa
     4141 tttcaaaaaa taaagaaatt ttggcttact gggcagaagg tgaaacaaaa gatggaattt
     4201 ctattggtta taatagagag tatggtggaa taagagaatt gttagcaaag gtttggaaaa
     4261 gtagagggaa aaaagtaaat atttatatac ctttgtggga tgaggaatta aaatattatt
     4321 taggaaaaga gaaagaagta aaagaaatgg ggactattct tcttatcaat ccaaatcttc
     4381 ttatagagaa tttaagtgga tattttatag aaagaggagt agaaattgat tatttaaaaa
     4441 gagaaaacaa aatattatta aaagcaaata aaaaagaaaa ggaatttaaa gataataaag
     4501 atttagcaag atttctttta catttaaata atgaagaagt atggaaaaat atttttccaa
     4561 taccattacc tttttatgga tttaattatg cataaacttc tcaaatggaa aaagtaatat
     4621 tttttattga tggcataaga atagtaataa aaataggaga tataacagaa gaaaaagtag
     4681 aggcaattgt aaatccagca aatacaaatc tcctaatggg tggtggactt tcattaaaaa
     4741 ttaaaaataa agcaggtatt gaaatagaaa aggaagttaa aaataaaagg tatataaaaa
     4801 aaggagaggc aatctgtaca ggtgggtgga aattaccttc aaaatatgta attcatactg
     4861 ctactatggg aatggatttt aaatccgatt acaaaacaat agaaaaatgt atggaaaata
     4921 ccctgaattt aagtgaaaaa atgaaaataa cttctatttc ttttcctgct ttaggatgcg
     4981 gaaccggaaa acttaaatta gaaaaggtct cacaaattat gattgaaaaa gtcatgaaat
     5041 ttttatcaag aaaaatattt ttagaagaga ttaattttgt tctttataaa cagagtgatt
     5101 ttgaaaaatt cctatcagtt gcagaaaaat atttgattga tataacaaaa aaaacatata
     5161 aaaacccagt tccaacagtt gatataataa ttgaatataa aaaagatatt attttaattg
     5221 aaagaaaaaa ttacccttat ggttgggctt taccaggtgg atttgttaat tatggagaat
     5281 cgcttgaaaa agcagcagaa agagaagcaa aagaagaaac aggacttgaa cttgaagatt
     5341 taaaacaatt tcatacatat tcacagccag gaagagaccc aagataccat acaatttcaa
     5401 cggtatttac cgcaaaagga aaaggcaaat tgaaaagtgg agatgatgct aaaaaagtaa
     5461 aaatttttaa taaagaaaat ttacctgaaa atgttgcttt tgaccataag acaatactaa
     5521 gtgattattt tgaatgtata agttcataaa ttatgaagtt atgaacttaa aaagttaagg
     5581 agttattaat tatgagcgaa aaatcaattt taagtggaat gagaccaaca ggaccattgc
     5641 atataggaca tttatttggt gccttaaaaa actggaagaa attacaggat gatggatata
     5701 aatgttttta tatgattgct gattatcatg cattaagcac agaatatgct aatccacaaa
     5761 aaataaagga aaatgtggaa gaagtggcaa ttgattttct catttctgga cttaatcctg
     5821 aaaaatcaac aatttttatt caatcacttg ttcctcaaca ctctgaatta catcttttat
     5881 tttcaatgtt tgttcctatt ccatatcttg aaagaaatcc tgtgtataaa gagcagataa
     5941 aggaattgaa ggataaggac ttacatactt atggtttttt gggttatcct atattacagg
     6001 cagcagatat tcttttatat aaggcaactt atgttccaat aggagttgac caattaccac
     6061 atcttgaact tacaagagaa attgcaagaa gatttaatta cctctatgga gaaacatttc
     6121 caattcctga agcattattg acagaaacac ctaaaattct aggaacagat gggagaaaaa
     6181 tgagtaaatc atataataat tgtatttatt tgaaggactc tccagatata ataaaaaaaa
     6241 gggtctcaac aatgttcaca gacccaaaaa gaatttacag aaaagataaa ggacatccag
     6301 gaaaatgtcc tgtatttatg taccagaata tttttaataa ggaaagggta ggggagatta
     6361 aaaaaagttg taaagaaggt gaaataggat gcactgattg taaaaaggaa ttaggagaaa
     6421 aggttgttga ctttttaaaa gatatacagg aaaaaagaga agaactttct aaaaataaag
     6481 aatatatctg gaaaatttta aaaaatggta gtgaaaaagc aagagaagta gcaacaaaaa
     6541 cattaaaaga tgttaaaatt aaaattggag taaattatga ataaaaatat tgaaaaatta
     6601 gtaaaggaag ggattaagaa aatttctcct tatgaaccag ggaaaccaat tgaagaattg
     6661 cagcgagaat ataaggtaaa aaaaacaata aaacttgctt caaatgaaaa tccttttggt
     6721 ccttctaaaa aagttaaaaa agcaatagaa aaaagtatag aaaatatcaa tagatatcca
     6781 gatggctcat cttatgagtt aaaaaatgaa ttatctaaat atttaaaatt acctgttgaa
     6841 aatataatga taggaagtgg ttcaagtgaa gttatttctt taacacttga aacattcata
     6901 aaccctggag aagaaattct ttatccctgg ccgtctttta caatgtatag aatttttgct
     6961 ttgaaaaata atgctattgg agttgaaatt caacttgaaa atgatttttc atacaatctt
     7021 acaaaatttc ttgaaagaat tactccaaaa acaaaagtta ttattttatg taatccaaac
     7081 aatccaacag gaacaattat aaagaaaaga gagatggaaa attttataaa aaatttacca
     7141 gacgatatta ttttaatttc tgatgaggca tattttgatt atgttgaaag taatgatttt
     7201 ggaagtgctt tcccattttt caaagaaaaa aatattatta tatgtagaac ttttgctaaa
     7261 atatatggac ttgcaggact tagaattggt tatggaattg ccaataagga tataattggg
     7321 tatattgaaa gaataagacc tccttttaat gtaactactc cttctcaaat ggctgcaatt
     7381 gcatctcttg atgaccagaa atacattgaa aatgttaaaa agaaaacaat aaaagggaaa
     7441 aaatacctat attctgaatt tgaaaaaatg ggagttgaat atacccattc tgaaagcaat
     7501 tttatcctat gtaaatttgg gaaaaataca aacaaaattg taaaagaact tgaaaaaaga
     7561 ggaataatag taagaggtat ggcttcattt ggtcttcctg aatatataag gattacaatt
     7621 ggaacagaag aggaaaataa aatttttata aataacctta aagatatttt aaagaaataa
     7681 cgccagtaaa ggggaggagg aaaaaaatga ttaaattggg tataaatata gaccatgtcg
     7741 caacattaag acaattaaga agagaggtat ttcctgaccc tgttggtgca gcagttatat
     7801 gtgaacttgc tggttgtgat tctattgtat gccatttaag agaagataga agacatattc
     7861 aggaaagaga tttaaaatta ttaaaagaag tcgtcaaaac aaaattaaat ttagaaatgg
     7921 ctctttctga agatgtaatt aaaatcgcat tagaaacaaa accagaccag gttacaattg
     7981 ttccagaaaa aagggaagaa ttaacaacag aaggtggact tgatgtgata acgaattttg
     8041 aaaaaataaa aaaagtcgtt gaaactttcc acaagtcagg tataaaagta agtatattta
     8101 tagaaccaga cctcaatcaa atagaaaagg taaaagagac aggagcaaat tttatagaaa
     8161 ttcatacagg aaaatattca atagttaaaa cagaagatga aatttataat gaattaaata
     8221 aaataattaa aggtactgaa ttcgcattat caattggttt aagagtaaat gctggacatg
     8281 gacttgatta taaaaatgtg acaccaatat gtaaaataaa tgggatagaa gagttaaata
     8341 ttggttatag cataatttgt atgtctgttt ttactggatt acataaggca gtaacaaaaa
     8401 tgattaaaac aataagagaa aatgaaagaa aagattaaag agtgttttaa agagccagta
     8461 cggggcaaaa taaatctttt taatttgttg atagggggtg ttttaacctt cttccccatt
     8521 gtaaatctta ttccacttgg atatttaact gaaaaattaa gaagaaagtt agaaaataag
     8581 gaaaaagtcg ttggatggga aaatttatgg tatttaatga aatcagggtg gaatactttc
     8641 atcttgattt taggatactt ctgtattgcc atttttttcg tctttttagg ggtaatcttt
     8701 atatttcccc tttccagagg caaaattatg tctatttttt tcttcagagg catcgtttta
     8761 attaatatag gaacaattat ctttctatta gggctttttt tcctcccatt ttcaatacct
     8821 gtatacttag aaacaaaatc attaaaaaaa gcatttgata taagagaagt attatctcgc
     8881 atccttttaa aattaaatga ttatcttgtt ctatatataa caattatagg agtcattgtt
     8941 ttttcttttg taataacatt tcttttacta aactgggtaa tggggttatt attaggcggt
     9001 tttttatatt tttatgctgg ttccgtcatt ataaatttaa tttcaaaatt tttccccaaa
     9061 aagtatttta cagttcaact tccattttaa ttttaaataa ataaaaaaaa ttttactttc
     9121 agaaattttt caataattga tttttaaaac attctataat tttattaaaa ttctcatagt
     9181 atttctaaaa atataaaatc taattatcaa ttctttatta tcttaatgat tttcaaaagg
     9241 atatagaaaa acagtcggat aagatatatt atgttaactt tttattgttt ggcaacttta
     9301 ctaaatcagg gaaaaatatt attatttgtt ttttttaaaa aatttatttt attaaaattt
     9361 tttctgagat taattagaac atattattga aaatcaataa cttatgtttc aaaaattctt
     9421 acaaatataa ttgaaatttc ataaagaaaa attaaaagaa ttgcaacaaa agtttgagta
     9481 attatatcag ttgtaggagt tattatggca gataaaataa aagctccaac aattgcatat
     9541 ttcctttttt gttttagaaa atttgactta actaaaccaa tagcagccag agcactaaaa
     9601 aaaactggta aattaaaaac cagtcctaaa agaatcataa aaataagcaa aaaattcaaa
     9661 tagtacccaa tagaaacaaa aggagtaaga actcctttac caaatgaaaa taaaacctta
     9721 attccaagtg gtataataaa atagaaggta aaaattaccc ctgaaaagaa aaaaatagga
     9781 aagaaaatgg agataacctt acttattttt ctctcttttt ctgtaagtgc aggagataca
     9841 aagagataca actgaataat aatgtgtggg attgagataa taccccctat aaaaacacaa
     9901 attttcagta tggaaaaaaa tggttcaaga ggtctggtaa aaataacatt tttttgatat
     9961 tttttcaggg gtataatcag aaaatccaaa atttgggagg aaaaaaagta agcaattact
    10021 gaaataagta cccatataac aattatccag attattcttc ttctgagttc ttcaagatgt
    10081 tcaataaaag aatattcatt ttgatttttt ttctgtgtct tcatcctttt caatctcttc
    10141 ctttgtctct ttaagccctt ttttaaattc tcccaatgct tttccgattg acctacctat
    10201 ttcaggtaat ctttttgccc caaaaagcaa taaaattatg agaagaatta aaataagttc
    10261 acccgctcct atattaaaca tattacctcc ttaaattttc aatcgcactg ctccttttgt
    10321 tgaaatacta attgcacttt ctccaataac aggacaattt tcctcacaca atccacaacc
    10381 aatacatttt tcataattaa catatggtct tcttttaaaa tcaaggaaaa ttgctccttc
    10441 tggacaatat tctaaacata caaagcaatc tttctcttct gtccagacaa gacatctttt
    10501 tttatcaatt tctgctgttc caatttttaa tttttcatct atttttttta ttgcctgcga
    10561 tggacaaaca tctccacatg ctaaacacat aatacatcca ccttttcttg gaataatttc
    10621 aggagtccca aatgagaaaa tatctttatt tattggtact gaaaacaagc aatttgaagg
    10681 acagacctta aaacactctc cacaccttat acatttataa ataaattcat cctcttctat
    10741 tgctcctggt ggtcttataa tactcttttt tgaataggta ttttttccct taaatttata
    10801 tctcccactt actaaaaaaa ttgaagaaat aaataaaaaa tttttaagaa actctctcct
    10861 tttcaatctt tcttctcctt ttttttatta aaaaatttct aagttttact ccaattaaag
    10921 aataaaaaaa acccaacgga catatattta gacaccataa tctattccat aaaatactca
    10981 atagtaaaat aaataaaaat acaaataaat accaattttt aaatacaaat gcacgagtaa
    11041 taatgacaaa agggtcaaaa aaccaaaaaa aattgggaat tttaacaaaa ttgactaaaa
    11101 ttgcagtaaa aattatcaaa ataaacaaaa atatgtttaa ttttttcaga aaaataggcc
    11161 ttctccttaa aaatttccat ttttttctta aaaaaaccat atttaaaatg tcaataatac
    11221 ctccaaatgg gcaaatccac cagcaaaaaa aattcccgaa taaaattaaa agaaacaaaa
    11281 taactaaaga gggaaagaaa taataagtga aatggcaggt agagattgtt gtaaaaattg
    11341 ccaatagtgc atcaagtcga aaaaataaat tgacaggaat tacattttta attagatatt
    11401 catttcttat caataaataa aaaaatataa atataaatat aagttggcac aatcttctta
    11461 aatatttttt aaacattttt tatattcact ttatttaagt cagcaatacc aagtcccatt
    11521 tcttctgcta tttttatatg cgggatatta taaggatctt gtccaagtaa ttttgttcca
    11581 taagcatcaa gagatacaat atcagttcct gctgcaacaa ttcctatatc tttaacaacc
    11641 cctggaccct gaggacccat tgttaaaagg atttttgtag catccattat agttaaggat
    11701 ggttttatat aataagtaat ttctgctata catctatgta aatcagttct atgaaaaaag
    11761 cccctatctt taacaattcc cattaagttt ttcatcccaa tagttacttt tgaaccacca
    11821 tgtactttaa caataggaac atttataaac acatcagaat caagaacttc ttcaagaatt
    11881 tcagcatttt ttaaaatttt tgttccttct atatttacct gtttaaattt tataggtggt
    11941 ttcataattc caccaccctt ttcaaccacc tctgcaatac ctgatatctt atatgtcacc
    12001 tgccagggat tacatggatt atcaataact ataacttctg aagcccctgc ttttttacac
    12061 aattttacta tacctaatac aacatctgga tttgtagttg ctgcctgttc aggggttcta
    12121 ttccatgcaa tattgggctt tataacaact ttactacctc gcttcacaaa cttctctatc
    12181 ccccctaccc cctttattgc tctataagta ttttcctctg ggtttcctcc cttcgcaaca
    12241 taaatttcac ttcctttact ctgtgcataa gataattttc caaactttaa gtgccttgaa
    12301 atttcaaacc cagcaattgc taaaaatgtc ctttttaaaa attcccttct ttccatttct
    12361 tccccctatg tttttcattt tcattaactt atattccact atttaaagta atttcttaaa
    12421 tcaaatttta tatttatatt ataccattaa aatataaaac aaaaaatttt ataattttta
    12481 ataaagttat cttatattat aaaaaattcc aatttatttt cgtggaaata gataaaagaa
    12541 aaaaaagatt tatttctagg caaaatttct tactttaaaa aatacgggag aaaaaaataa
    12601 aaaagggtat ttttgtctat tttgatattt ttagtggtta atttcacctt ttatgtggtt
    12661 cttttttttg tagtatatcc aataaacaag agttataatt acaacaaata taataattaa
    12721 aattgtaatt tcatttgaat attttttaat taagtccttc ttatctccta taaaatatcc
    12781 caaaaaagtg agaatactaa cccatatccc tgccccaacg attgtataaa cagaaaaaat
    12841 aaataaattc atttttgcaa gtcctgctgg taaagatata tactgtctta ctcctggaat
    12901 taatctacca ttaaaagttg aaatatgccc atgcttttta aaaaatcctt ctaattttcc
    12961 taatttcttt tcatctataa atagatattt accatattta attaaaaact tccttccata
    13021 cttttcagcc agaaaataat taaataatgc ccctgcataa ctaccgataa ctccaaaaag
    13081 aattgaaacc cataatgaaa gtttacctaa ataagataga tatccagcag gaatcataat
    13141 aacttcactt gggaatggga aaaaagaact ttctaaaaac atcaatacaa aaatcccaaa
    13201 atatccccac ccccctatgc ttctaactaa aaaagcatta aattcattca tttttatccc
    13261 ttttctctat ataataacat tttacatttc ttatgagaag atttattatg tctaattcgg
    13321 tatattgact aaaaatataa ttggtttatt tttatgagga aagaggcaaa aagatggtta
    13381 aaagaagcag aatttgatat taaagcggca aaagataatt taaaagaggg caacttcaat
    13441 atatacttat tctattaaaa ttttattgaa cgaatgtaaa aagttaaaag aagtttttca
    13501 taattgttag aatatgggaa aatattggaa atgacagaca ttcccattga accgctctgt
    13561 tatacttatc aggaatttaa taaattaaaa agggaaaata gttttatcaa ggatatttta
    13621 aaacaaacaa aaaaacttta attctataac ttcttaactt acgaatttca taacttctta
    13681 atttattttg aaacaaattc tggctaaata tgaactgtta aaatggatct ttcttattta
    13741 atctgcattt aaagtattta atttatattt taataacctc acaattttaa taatttatgc
    13801 ttagagatat ttcttcttct tacttaattg ataaagtatt ttcatcagtt tattctgtct
    13861 atacttttta tccatttttt tctctttatt ataccaaatc ttcactccta actttaattc
    13921 tttcctatct ctttgagtat cactatctta tccagaggct cgcccgtagg gtctacgccc
    13981 gaggggattc tttggacaaa tattactgca acttttttaa gaaagaacct aaagttttat
    14041 cattactata tttttgatgt caaattagta taagtcatta attaggcaac aattggaaca
    14101 ttaactgctg ttgggttttc ttctataatt tttttaagta aatctaaata ttctttctga
    14161 atattattat atttttcttt tatttctggg tctgcttttt cttttaattg ttttgagata
    14221 ttatattttt ttacctgtaa ctctccaaac tttaaaccca cctttaaaaa ttctactctt
    14281 tcaaaaactt ttttattttc tatgttttta gaaatatttt ctgctttttc taaatatgaa
    14341 tttaactttt gaaaaatatc atcactatat acttctaaga tatttatttt cttttcagcc
    14401 gcttcattac agacattttc aataagagag aaatactttt taatatattc agatgctccc
    14461 tcaaaccctt tctcacaata atcatctatt agggaatcta tatttatttt atcaggattc
    14521 cagtgtgatt tggctaaaac ataatatgtc aaccccatca atgcccagtg ctgttcacaa
    14581 gaatcaaaat cggttcctat cattccagaa tcatataaat acttcatatc ttcaaatatc
    14641 ttatgagaaa agttctgtgg aacaatatta tctctatttg caagaagaga attaggtcta
    14701 taaaacattt tgtttccata ttttgaccat tcatcccaat cttttaatat ttgtttcctt
    14761 gcctcgtcat cagtataaga cccattaaca aaacatatta ttaaattagg atgtggtttt
    14821 acatttattg gtggtgcata ataagcacta taagcatcat atcccaaatg tttatcagga
    14881 aatacttttg atactccttc tgcaatttga ttggaaaacc ataaaactct atctgtaaga
    14941 gatacatatt gaaaaggtat tttgtttcct gttgtataat cataaagagt tattggtcgt
    15001 ccttctggtg ggtctaattt cctacaattc tcacacatac aaaaagtcgt tcttcctcca
    15061 tcatttaagc caattgctac accatcttgt gttggttttt ttcctaattc ctgtattttg
    15121 tttttaataa tttcatttat tagttctcta tttgaaagac ataaacgaca gcggtcaggt
    15181 gataatgatt ggtctctaat tccatttggt tgaagtgcaa accaatcagg atggtctttt
    15241 ccatactttt cccaataatc accataagaa tgtgctctat atacttgttt atgaccaccc
    15301 attctatgcc aagccaacca tccatcatct gtttcctctg attgagttgt cttagttaag
    15361 atgtttttat atttctcacc gtcaaagttt aatttcttca aaccatcaag tattctggaa
    15421 cttttgggag aggtaaccct tatatttctt gtccataaac gtggagattc ttttatatct
    15481 aattttgaaa tatacaatgt attcttcttt ggaataacct ttcctaattc accaggccat
    15541 aaatatctac atcctaattc ttctaaaaat gtataagttg catgtcttaa cccttgctcg
    15601 tcattaccac ttataacaac tacattacct gccgtcttta ttatcatccc ttctggacct
    15661 aatccctcaa aagaaatacc taacttatca agaaaatcat aactacctat taaaataaaa
    15721 ttaaagtttt tcaactcccc atttacttct atccctgaaa gagaatatct tggattcttt
    15781 aaatcagaaa tctttaacat agaaatttca gtatcagtta ttttttcaat atgttctttt
    15841 attatttttg ctgaacgact ttcttttgca ctaggatttc ctgacagcaa aattcctgct
    15901 tttgcctgtc cattttctac taaaattagt tgattatccc catacattga cttacaaaaa
    15961 atacttaaaa ataaaccaca gacagaaaaa aataatagtt ttttattaca acttttcttc
    16021 attttttgcc tcctaatggt attggttccc atttttcttt ttttatttca ccacgagcag
    16081 ggtttccttg taatattgcc tgaaagagtg tattacgata ttcctctact tcagggtcta
    16141 attgtttttt ctggtataat ctgaaaagtg gtgtcccaaa ccaatacact ttcccttttc
    16201 ctattatatt ctctgttata aaaggtgata aaacttcccc attttcatca acctgcactg
    16261 caagaacttc tgcacttgat acattactta ttggtgttgt atctgctatc caatcatatt
    16321 cttccttttt aagatgagca gttattggtg attttttatt cactattttt actttataag
    16381 gactttttcc tctcggatat cttggataat aaccaaatcc taaaagatga gatattgccc
    16441 ctaaattcct accttctgct aatccatcaa aagcacagca agtaataatt ataactcctc
    16501 ctgattttaa atattcttct actttcttca gaacttctgg gtctgtccac atattccctt
    16561 tattttcttt gacagttggt aattgcccaa ttctctctgc catatatata actgaaaaat
    16621 tataatattc acttggttgt aaccagtcat caaaaacttt atattcaata ctctctggtt
    16681 taaaaattct gtattcaaca gcagttattg gacctccata ccctaaaatt gcaactggtt
    16741 ttttcacctc atccgctaaa gcaactaaaa atatacttaa aaatataaac aaaattataa
    16801 cttttttcat ttccacctcc taaaaatcca ttgtggttaa ttatacttta taaaatcttc
    16861 tttaaaaaat aactaaattg cttaatacct aataatactc ccctatcatc tcctcctaaa
    16921 ataataatta ttctcttgcc atcggcaaac ggattgtttg tttctatcac tgcataatca
    16981 ccttgttttg gatatgaatt tgtataacgc tcttttaaat agtcctcttt attgattatt
    17041 tgttgaatta ttgggtcagc aagagttcct gcaacaataa ctgatgtctc tttaacaact
    17101 ttttccaatg gttctgcttt tatttcattt aatgtgcctt gtgaaagttt atctatcagt
    17161 gggaacatat ctgctgatga tatccctttg ccaatagatg gaaaatacac caattctcct
    17221 ttatcaacta attttacttc tccccatctt atatcatatt ttagtttttt acctgtcttt
    17281 tctaatatat cttccaacaa atccaataat accttacgcg cctctatacc ctgttcaggt
    17341 gtgaaatctt ttatatcagt cctttccaaa ccacctttaa ttggttgtct aaccatgtga
    17401 ataatggcat cttcatcttc acttacaatt ttagataatt gttcctgcca tttaataagt
    17461 tgtgtagttt caactaattt tatctcctca actgctttta taagatgttg tgcataacga
    17521 tatagattag cattatctat accatcacga agtccttccc aagcaggtga agaaagtggt
    17581 cctactggtg ttgggaaaat tattaaagca ccaagtcgtt ctccaccacc tcgtaaatat
    17641 tcatgattat gaaatccagc aagattatgt tgacctatat accagccctt agagataaaa
    17701 tattgatagt ccaacgatga agttccacta ccaatatatt cccagatttc atcagtcggt
    17761 cttaaaattc cttcttttac ccacttttca acaacatccg gctctgaaaa actgccacac
    17821 tgatacatac gaaaataagg tgatagaact tttaattctt ctggctgccc aagaagtgca
    17881 tccacagtag taaatggctg ccaccctatt tcctgtaaaa ctttaccaac ttctgccatt
    17941 ttgggatata tatctgttgg ttgctcatca agatttttta tgaaaatact attcttagga
    18001 taacctcttt ctttcacata ccaatacaat tcttttaaag cccactttgt aaatccttgt
    18061 ggaacttcag tagaataata ctctgtctta aaacgaatta gcccttgttt aattccttca
    18121 tatatccaac cattataata actaaaatct aaaaatttat tctctaattc tttctcatct
    18181 acatttttat aaagtttttc ataatttaaa aatagttgac cactatctct tgctcttgca
    18241 ttatttaaaa tccatcttct atctgcttga agaaaatctg cattatgctg tcctaaatca
    18301 caaagataac ttttcagtcc acgcaaattc tcttctggat gaccaaaact tccaatatac
    18361 ccttctacct ctaaacttac taattggtct ggcatactaa tttgtctaat ttttatattt
    18421 acaggaattt tgagagttga acatttatca gggctaatta tgatatcaaa aatatagtct
    18481 cctggctcta acttatctgt tgagaaagtt aaccataaat attgtttttt atttccttca
    18541 aaccttttat ttgaaactaa tttgtgtaag ataaatggat cctcatatcc ccatatactt
    18601 gcatcaaatt cttctacttc ttcaatcctc atttttggat atatttcaac tggaaaacct
    18661 gtcttgatat ctcttaaatt cgttgctccc caagaaacca ttcctatatc ttctttcaat
    18721 gggtagtaaa ctatcctaaa tgactcaaac tcacctttta ctaaatctaa attatatcct
    18781 tttaataact ttcctgcttc ttcccctatt tcactatata ccatataacc acgtttttta
    18841 tcttcttcac ttggttgaga tatttctttt tcttcttctt ttttccaacc agaaatgtcc
    18901 tggtattctg atattgataa atattgtatt ttacgctcat aaggaccatc aacaaaatga
    18961 cctgctctta atgagagtat atcttcaaac tgccattttg ttttaccagt tgtaattact
    19021 tcccccttta atacataaaa acctttttca ggacatccag ttgttatata tttggtttgc
    19081 ggtttatcta ctacaacatt tttaaatgat atttctggga tattttttac tatattccca
    19141 ttaaaatctt caagatgtaa aattattttt acatttgaaa gagatttcca tatagatgat
    19201 atttttattt ctaacataag ttctttttct gtttgactaa tatctcttat atcaagaaga
    19261 taatcgggtt ttgcatctat gagtgtctca aaaccatgtg ttagaattat caataatttg
    19321 tatgtcactt tcttgcctgc tggaactgat ttgtaaaacc actcataagt aggacgagga
    19381 attccccctt tccacatgta ccatcctgta tatccatcat ctaaatgaac aaaagtcagt
    19441 cctgtaagtt ttttcttatt taaaaaagca tgccaaggtg ctcctacttg tttctttatc
    19501 ttttgagaaa attctggcat ttgtcccggg ctttttgttg gactaaatct gcgaactcct
    19561 tttattgtcg gtacacagta tatatcttca tctgaaacta cttctcctcc cggtaaaatc
    19621 ccattcctta cagcagtatc gttataagtc atctttttct gactctcatt tatatatgta
    19681 taacttaccc ctattattgg cactttatca taaaacgtta attcttttat tacactctgt
    19741 tgcttttctt catctttata acttaaactt atttttatct catttttatt tgaacttaat
    19801 attttaaaaa catatggttt attttcacga cctcctttat catcaagaag tcctccccat
    19861 aatgagcagt tattagaaat tactgaatgt aaaagttctg tatttgtttc tttatctatc
    19921 aaagaaagaa tcctgccacc tgcatctgga actattgtaa ctttatagta tggtgtttct
    19981 attactttgc ttttttgtcc agttggttga aatatatctt cctttaaaat tattcctcct
    20041 tttgcagtaa acaagaaact tataaaactt aatactaaaa ataatttaaa attccttttc
    20101 ctcatttttt ttctcctttt attggtcaat ccaaccaggg acgccaaaat gcattccaat
    20161 aggacttata tctatctgcc catttaggta tatcttctac cctttttggt gctacatgtc
    20221 catctaaaaa aagtatattt gcataaccca tatgatgaaa acccatctca tcaggataag
    20281 catcaggagt tattctatat gtgccagaac tattttttgt aacatcggaa attaaaaatc
    20341 ctgttgtaga atgtttcatc ctatccaatt tccaaaagaa acctggtaaa tatgaattca
    20401 ttgcataagt catccaaccc gttccacctc cagtccattt tgtatgagaa ggacaaatcc
    20461 ataccctttt tatatcatat ctcttcaatg gtatatatgg atcaatatat ttatcccaag
    20521 aagaatatgg acttccaaaa ggcctgtatg caggacacca cccatcataa tcctgggcat
    20581 acataaaaaa ccctaatcct atttgtttta agttattcat acagcttgct tgtcttgcct
    20641 tttctcttgc attacttaat gcaggtaata acatagcggc aagaatcgct attatcgcta
    20701 ttaccacaag caactct
//
LOCUS       DAOOVW010000030        19663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_1870_length_19663_cov_8.696348, whole genome shotgun sequence.
ACCESSION   DAOOVW010000030 DAOOVW010000000
VERSION     DAOOVW010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 19663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19663
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1870_length_19663_cov_8.696348"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..1942)
                     /gene="priA"
                     /locus_tag="PLW95_05375"
     CDS             complement(146..1942)
                     /gene="priA"
                     /locus_tag="PLW95_05375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOV22093.1"
                     /translation="MNIIEVVFDIPVDKKFEYLIDKEIAPFVRVLVPFGKTKKVGFVV
                     SVKKGEKQELKKVIKVYDKCPIINSYLYQLANFISETFISSLGQAIFSIIGTLPIRHT
                     SFDIKEKKGKKEKGKRKIIIFKKEDEKKNYYLNLIKENIKDGSVLFLLPEVEIAKEYF
                     SFLNTEFKNCILYYGEMKRKEKGEKWVEIANGERNIIIGTRICIFLPISDPSSIIIED
                     ATNQAYLERQTPKYETLKIAEFENEKIGTSLYIGENCLSVENYLDIKNGRYGSVVLGK
                     EENPSQIYILKLRKGLMDKNLTFLTKEAVSLMEETIIKKKKVAIVHNRKGGSKILICE
                     KCNYKFTCKNCSTLLTLSDDGKTLFCKYCKTYFEFEKKCPECGSKKITFKSYGIEKMA
                     NVLKQTYKNLKIEKFTGESKIIDDEFDILIGTGIIEKVIKKFDFGLIIFSNADLYLNL
                     NDFSAEEKFFILVHKFLSVTQNTCFLIIQTGSPELEIFKAIKERNEDIFFEKEIKIRK
                     QIGYPPFSKLLKIEIKGSKKTPLEKRKKMVEEIIKEDNLKIIYSGESFPPVIGKKHIW
                     KFLIKIDDKIPKKIKELIDGKFITASFNPSHI"
     gene            complement(1929..2789)
                     /locus_tag="PLW95_05380"
     CDS             complement(1929..2789)
                     /locus_tag="PLW95_05380"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOV22094.1"
                     /translation="MGNKKITLIDGAIGTYLTKKGYKGLTPELACIERDDLVEEIHTE
                     YVESGAEIILTNTFGANKARLSKKKLEDKHQIINEKSIEIATKVKNKKNILVAGDIGP
                     TGELLYPYGNIKQEECEEIFLSQAKFFIGKVDIIVLETFTDLSELEIAYSILKKNFQI
                     TIIPCLSFQIGKEYRTVMGNTIEDYISWTRNIEVKIIGINCGVGSEQMKEIVKKIYSL
                     TEKSLWVKPNAGIPKLLHGEVIYPENKEEFVENCLFMCKNFPVEFIGGCCGTDPSYIK
                     HLKEKINEYN"
     gene            complement(2799..3167)
                     /gene="queD"
                     /locus_tag="PLW95_05385"
     CDS             complement(2799..3167)
                     /gene="queD"
                     /locus_tag="PLW95_05385"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013910125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HOV22095.1"
                     /translation="MYKIKVIGGFSSAHRLKNYKGKCEHLHGHNWKVEIIVSGTTLDN
                     TGLLIDFGILKKKLDFILKQLDHKNLNTIPFFNKTNPTSENISYYIFTKLKELLKNLP
                     IKLEKVIVWENEKQCASYME"
     gene            complement(3160..3963)
                     /gene="murI"
                     /locus_tag="PLW95_05390"
     CDS             complement(3160..3963)
                     /gene="murI"
                     /locus_tag="PLW95_05390"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOV22096.1"
                     /translation="MKKNPIGIFDSGIGGLSVVKKVKNILKYEDIIYFGDTARVPYGT
                     KSKKLIEKFALDDVNFLLKFNPKVIIIACHTASSLAGQLLKRKFSSLNIIDVVSSAIK
                     KTIEITGKKRVGIIGTSATILSRKYEKLLKKHNKNISVFSMSCPLFVPLVEEGLFTAP
                     CTYFIAEYYLKRLKKKNIDTLILGCTHYPYLKKVIQETIGRNVNIVDPSYEVALNVKK
                     YLESNNLKDNKQTGKIKLFFSDISPYTEKVIRKIFPESGEYTLHNVPNV"
     gene            complement(3963..5072)
                     /locus_tag="PLW95_05395"
     CDS             complement(3963..5072)
                     /locus_tag="PLW95_05395"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOV22097.1"
                     /translation="MKKIKIISIFFVLLTCNLFSLNLNDAIKERKLPTYIIKDQSYIS
                     LNSLIDVIQPQSWGKIEDRVFIIYKGEEIKFRVGEDKVIFGNKTINLKYPIKEIEGEV
                     LVPLDNFSSIFSQVPETKEENPIPQRKIQVEEKSQKGNTYVILIDPGHGGKDTGAIGN
                     FGLREKDVNLDVSSRLVDYLRKYFGKSSNVKVYQTRDEDVFLTLEERVQMANTLNADM
                     FFCVHTNSAKYNRFDANGFETYYPRTKQRVENLPKIVNDEGILRDEATNEGMLTDILL
                     ELNTTNTIEESKILAEFVQEKLAERLLTPDRGAKEGNYYVLKYTPMVSVLVEIGFICN
                     PNIELNLKDAEVRQAIAESLGNAIIEYLKSRKIIE"
     gene            complement(5149..5952)
                     /locus_tag="PLW95_05400"
     CDS             complement(5149..5952)
                     /locus_tag="PLW95_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV22098.1"
                     /translation="MVEEIYKMENLEFSYGKNRVIKGINVSIKKGDFVGIIGPNGSGK
                     STFVKILTKILQNYKGNVFFKGTELKKISLPQLSKDVSFLPSSINVYFPYTVFEFVLM
                     GRYPHLSKFEKIRVADYEIVEEVLEKLGIKKLSNKKLFELSEGERQFVFLAQLFAQKT
                     EVIILDEPISHLDIGHEFKIMDILEKYNEKGITIITILHNLNISSEYSKCIFLFKDGK
                     ILKEGKVEEVIDYKTIEEAYETKVIVHKNPFSGKPYVFGIPEKFLKTNC"
     gene            complement(5946..6914)
                     /locus_tag="PLW95_05405"
     CDS             complement(5946..6914)
                     /locus_tag="PLW95_05405"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOV22099.1"
                     /translation="MKTKIKFLFVFLLFILSFVFSVLIGSESFSISSIFNPSILSELD
                     RTILFKIRLPRVITSFVVGAGLSISGVIFQAILENPLAESYTLGISGGACLGICIGVI
                     FDNFLIIPFCAFIGGILSISIVLFASNFKKFSNPSLILLGVVLNFIFSSLVLFLLSVF
                     KNEKFQQTFVYLLGDLSSTPFPLIKFLSPIILVLSFFSIVFYKTLDILSIGEEKSITL
                     GVEVEKEKRKILFIASLITALCVSLSGIIGFVGLIIPHIVRIIFGNLHKHLLPLSIFV
                     GGTFLILSDTLSRVIIIPLEIPVGVITGFWGGLFFLFLLFKGEKIW"
     gene            complement(7165..7965)
                     /locus_tag="PLW95_05410"
     CDS             complement(7165..7965)
                     /locus_tag="PLW95_05410"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HOV22100.1"
                     /translation="MKKIILILFLFSFFAITENLRIVSLCPYVTENLILLGEGNNIIG
                     LTLEDEEEYKKGKEIIGSMLQPNIEKIVFLKPDIVIASKEGNRQESVLKLNQLGIKTL
                     VLSELFSINDIFKNFIQLSEITGKKEKGEEIVKKAKKKLKNLKCKKRKSVFFVLGWKP
                     LITTGKNTFINEIIKLSGGENIFASIEKKWIQINIEEVVKRNPDVIIYIEMGENFDQY
                     IKKLSSVKAVKENKILEIEQYKIASPTPESIVDVCIKINKFINAQQGS"
     gene            complement(7962..8675)
                     /locus_tag="PLW95_05415"
     CDS             complement(7962..8675)
                     /locus_tag="PLW95_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007276743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOV22101.1"
                     /translation="MGHKKFSYGFTLIELLVVIAIIAILSSMLLPALSKAREKSRQSV
                     CLSNLKQLTLAFLMYVQDNDEYFPPSYYITYTSEIAWDFKTDTWWATYEKGIIGSYLT
                     KGIFECPTAKKIIKSYDRPFTGFAYNSTYIGGGYSIWTGQSDPPTKLSRIKNPSETIL
                     LADSGIWSTYTNEIIGNNYLRIKEDPYDFGPTVHFRHNGFANVAFVDGHVEAIKDKYN
                     ISSYDSSIAYLKSELYDLE"
     regulatory      complement(8776..8961)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8963..9634)
                     /locus_tag="PLW95_05420"
     CDS             complement(8963..9634)
                     /locus_tag="PLW95_05420"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOV22102.1"
                     /translation="MNNEKIKILGISASPRREISNTSILLKDILSGAEQKGAETEYID
                     LCNFRIEFCRACENCHKKIMACPIKDDASFLLNKILENDGIIFGSPVYLNHITGYLKT
                     FLDRSSHFLHCHRLMGKYTAVVATSGGGPQDMIGDYIKEYSISCGAQYVGGVFTKIPI
                     TEEVKKHANELGKAIVDAIKEKKEFPEQIQKINSHKEYFRKIIENRKDDWIEEYQYYK
                     DNNWF"
     gene            complement(9653..10402)
                     /locus_tag="PLW95_05425"
     CDS             complement(9653..10402)
                     /locus_tag="PLW95_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOV22103.1"
                     /translation="MEKERPGYDDTPLIPGSKYKVHDRNRPYPPVVAPGTPGTQEKAG
                     IPPSDAKILFDGKCLCGWVHLDGRKAEWKVENGYMEVVPQTGNIKTKEEFGDCQLHIE
                     WASPEKIEGEGQGRGNSGVFMMGIYEIQVLDSYNNPTYADGSAAAIYGQFPPLVNACR
                     KPGEWQTYDIVWLCPRFKGEKLISPAIITLFHNGIVVHYHREILGPTAHKQILPYKPH
                     PNKGPLMLQDHSNPVRFRNIWYRDLYLDNEK"
     gene            complement(10424..11035)
                     /locus_tag="PLW95_05430"
     CDS             complement(10424..11035)
                     /locus_tag="PLW95_05430"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOV22104.1"
                     /translation="MKKPKLRELKEGIISLFTPPYTTKFPYEPHIPPKRFRGRPEFNL
                     DECVGCGACANVCPSKAIEVIDDIGEEKRVLIHHPDKCLFCGECERNCITTKGIKLTN
                     KFDVSYFKEPEEVENKVEYPLVICKSCGSIIGTEKHIIWIYKKLGNLSYSNAVLVTNI
                     IKNLNIKTEEQEKVILPIQRTDIMKIICPVCKRTAFISDEKIK"
     gene            complement(11049..12764)
                     /locus_tag="PLW95_05435"
     CDS             complement(11049..12764)
                     /locus_tag="PLW95_05435"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOV22105.1"
                     /translation="MNKETLILLPTAICFIISLIHILFYKLSRKKRCWISILGGLVVL
                     LSTIYIVCNCTWFLLLGGISSFFAILISFLGLLVIIYSQKYNWQSTFVYDCLLFFFIG
                     SMLGTTLSPNLILFYSFWEFMTFSSFFLILYENTKEAKSASIKYLIMTGAGSIFLLFG
                     ILGYLSGIAGQSIFWKHLLFFSIIIGAGIKAGLFPLHSWLADAHPAAPTPISALLSGI
                     MIKIGIYALILFYFEIFSPSWSIGWQTIMIIIGCLTVLTGCLLAIIQNDIKRLLAFSS
                     IGQIGYIFLGIACGTNLGLLGALYHTLNHALFKGLLFLAAGVIIKKVGSRNLSDYGGL
                     GEKMPFTFFAFFIASLSISGIPPFNGFISKWIIYQALLEKGTVLTTVAFLIAILGSCL
                     TLAACLKVLNDTFLGVRKRENIQKESRISPFFSIPMGVLSVLCFILGVFPDFFMNNVF
                     SKIVFAPEVNISLINIVNFYGILLVLVFFVIFIFYKKIIGKMSKKEIFTGGEKLEREI
                     ASFDGSQFYLTLRELPVLKTIYNWEDKGYLDIYNIFLKKIVPWLKKVFKVFEISFPTN
                     DKNKT"
     gene            complement(12757..14136)
                     /locus_tag="PLW95_05440"
     CDS             complement(12757..14136)
                     /locus_tag="PLW95_05440"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOV22106.1"
                     /translation="MNNIILALVLPFLGAFCIKTFLKRKPIIVATFFTLLQFISLLLI
                     SKTGTTVYFLYGIKFSCDFLSYIFAITSVIIGIFIFLYSIDYFSDENEEFKNNFSFWS
                     LILIGCLTGVVFSDNLFLLFIFWELAEFCFYKLIAFYKGENENSSKNNIYLVTKVCSA
                     IIILFSFILIYINTNSLSLLEMKGSCIPPYVFYLFFIGIMIKSATFPFHYYIPSTTIA
                     PTPVVSFFNTILVNISLYGIAKLFIKTLFITSSVSWIGYIALFSSVLCGVLSFIEIDI
                     KKILTYSTISQIGFMICGLLSFNEIAFKGIMVFYVANVLGKGCLFMCAGILEKVFKTN
                     DITKISVSAKKMPVTFTAFLFSSLSIIGIPPFLGSSGKLNIIYGMLKSGNIYFAFFSI
                     ITLALTLFYILRLFKYIFIRTTEQTDIITENNERKYKFMMFCVLFLCILSLLGGIILT
                     LLTGEELFE"
     gene            complement(14129..15331)
                     /locus_tag="PLW95_05445"
     CDS             complement(14129..15331)
                     /locus_tag="PLW95_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HOV22107.1"
                     /translation="MEKNNRKKVVVPIGPYHPLLEEPEYFNLYCEGEKVVDVEWVAGY
                     NHRAIEKLSESKHWDQVTFLVERICGICSTSHPIAFCNAVENLLEIDIPERAKYIRNI
                     IGELERLHSHLLWLGLAGHFLGYNTVFMWAWKYREPILDMFETITGNRNHYAMLKIGG
                     VRRDIEDEDIPLLKKTLDKTKKKVGMLTKAVLDDPVLAARLKGVGVLTKEDIKNYGAV
                     GPIARASGIDIDVRKDDPYAGYNLVEWKVITCENGDVFDKAVVRLGECFESIKIIEQS
                     LEAIKGKKGEICKEVREIPEGEGIGHHEAPRGECFHYIRSDGSNSPVRHKIRAPSYTN
                     IPTFKTSCINQTISDVCIILAGVDPCYCCTERVAVYDIEKRNKKLYDFQDLVKLSIEK
                     TKKLRENE"
     gene            complement(15318..15761)
                     /locus_tag="PLW95_05450"
     CDS             complement(15318..15761)
                     /locus_tag="PLW95_05450"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOV22108.1"
                     /translation="MKDILKEKLGNLIKEIKVVNEKRIYITIDKKDIKKATEIMFKYM
                     EGRYIIVTGIDNFDNFELLYHFSFDKKGGVIVSLKTFIDDKKRPEIESLTCLIPGISY
                     IEREIWELLGINFIGHPDLKHFLLRDDWEEGNYPLRKEVKTDGKK"
     gene            complement(15758..16174)
                     /gene="nuoB"
                     /locus_tag="PLW95_05455"
     CDS             complement(15758..16174)
                     /gene="nuoB"
                     /locus_tag="PLW95_05455"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HOV22109.1"
                     /translation="MNIKLKALKKSLWVYHVSGGSCNNCDIEILDLLTPRFDIERFGM
                     VLVGSPRHADVLLCTGIINKKCFERVKGIYEQAAKPCVVVAIGACACSGGVFRDGYQM
                     AGPYNNVIPVDIYIPGCPPKPEAMISAIVKFLEKLK"
     gene            complement(16176..17105)
                     /locus_tag="PLW95_05460"
     CDS             complement(16176..17105)
                     /locus_tag="PLW95_05460"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOV22110.1"
                     /translation="MTFTYYLWYFILGGIFLSIGGCIANWIERKLTARIQFRVGPPWY
                     QNFADFVKLMGKEVIIPEGANTPIFILSPIISFISVIICGVMAILVQKGIFTLPGDII
                     LFVYFLTIPSLAIILGASASGNVLSAVGLSRELKLLLGYELPFICAIIISAIKAGGTT
                     SLLTILNVQQQTHIFIGSLSGFIGFLISLFVITGKIGIVPFDTAEAETEISSGVFMEY
                     SGILLGLFKLTKYVLLIVGPVFIMTLFLGGFSYSSPIAIIISLLKYILIVFLIVLIKN
                     TNPRLRIDQAMRFFWGHLTFLGIIGIILSFLGW"
     gene            complement(17102..17590)
                     /locus_tag="PLW95_05465"
     CDS             complement(17102..17590)
                     /locus_tag="PLW95_05465"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HOV22111.1"
                     /translation="MKKLFIDYDKLLICKCQLKCSYILHPENNGIINLKEQIAFLLAC
                     RKCEDYPCVNACPNQALKRENGVVKRSNILCVSCKSCLIACPFGTIVPDIVSYLTSKC
                     DFCINRLKENEIPVCVESCNCGAIKFVDEKDIEKTKITFEVGKILIVNVYNYLSLQGI
                     EK"
     gene            complement(17592..18422)
                     /locus_tag="PLW95_05470"
     CDS             complement(17592..18422)
                     /locus_tag="PLW95_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HOV22112.1"
                     /translation="MTENIYKPIKCEVIDVIEESPTIKTIVLKPEKQFNFLAGQFASL
                     TIPGVGEAPFTPSSSPFDTEKLEFTVMKTGRVTEKIHQLKKGDIVGVRGPYGKPYPLE
                     KFKGKEVLLVGGGVGLAPVRALFLALVHTYQNYKKIVFCCGAKSPSDYIYKDKLFGDW
                     QKLCEKIPLSFRITVDKKDETWKYMEGVVTVTLDALDMDIKNSVAVVCGPPIMMKFAT
                     LKLLQINFSDSNIYLSMERKMYCGVGHCRHCLIGDLYVCKDGPVFTYDQIKNKPNIWV
                     "
     gene            complement(18419..19435)
                     /locus_tag="PLW95_05475"
     CDS             complement(18419..19435)
                     /locus_tag="PLW95_05475"
                     /inference="COORDINATES: protein motif:HMM:NF028488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOV22113.1"
                     /translation="METLFITKQDCENFLKGKIKEKKVYAPIEENQNLFLNLIDENNV
                     ENIVYNKFRTVEPLKIFLLPIVEKIVNEIENFDEIILIGIASCDLKGLEILDNVFANG
                     EYKDPNYKRRRENLLIISTDCYQPSDTCFCEIVGTNPYPEKNFDINFSMTTDGFIVEI
                     ATEKGNEFIGKDNRFFNATVQQIDNRNVLREKTVKRIKEINEVFNLNNFQEKIKGVYE
                     TEKWEKINDIKNCVSCGSCNFNCPSCVCFFLEDTSEKENFEKIKFWDSCLFPGYARMA
                     SGATPRPNLHSRYANRLLCKYDYMVSNFNILGCTGCGRCISGCIGKIDKRRVISELVK
                     EVVK"
BASE COUNT         6756 a         3352 c         2259 g         7296 t
ORIGIN      
        1 ttatttgata aaaaaatttg aactttaact ttgcttttaa tatagtataa aactggataa
       61 atattcttat cagaaagaca gggttgagta aatacttaca tatattaaag tcagcacact
      121 ctatatttac ggcaaaatat attaattaaa tatgagaggg attgaaggaa gcagttataa
      181 atttgccatc tattagttct tttatttttt ttggtatttt atcatcaatt ttaataagaa
      241 atttccatat atgttttttc ccaataacag gaggaaatga ttctcctgaa taaataattt
      301 ttaaattatc ctcttttatt atctcttcaa ccattttttt ccttttttcc aaaggtgttt
      361 ttttacttcc tttaatttct atttttaata attttgaaaa tggtggatat ccaatttgtt
      421 ttcttatttt tatttctttt tcaaaaaaaa tatcttcatt tctttctttt attgccttga
      481 aaatttcaag ttcagggctc cctgtttgta ttataagaaa acaggtattt tgagtcactg
      541 aaagaaattt atggacaaga ataaaaaatt tttcttctgc tgaaaaatca tttaaattta
      601 ggtataaatc agcatttgaa aaaataataa gtccaaaatc aaactttttt ataactttct
      661 caataatccc tgtcccaatt agaatatcaa attcatcatc aattattttt gactcccctg
      721 tgaacttctc tatttttaaa tttttatatg tttgttttaa aacatttgcc attttttcaa
      781 ttccatatga tttaaatgtt atttttttac taccacattc agggcatttt ttttcaaatt
      841 caaaatatgt tttgcaatat ttacaaaaaa gggtcttacc atcatcagat aaagtcaata
      901 atgtggagca atttttacat gtaaatttat aattacattt ttcacatatt agaattttac
      961 ttccgccctt tctgttatga acaattgcta cttttttctt ttttattatt gtctcttcca
     1021 taagagatac tgcctctttt gtaagaaaag ttaagttttt atccattagt ccttttctta
     1081 acttcaaaat atatatttga gatgggtttt cttcttttcc aagcacaact gaaccatatc
     1141 tgccattttt tatatctaaa taattttcaa cagaaagaca attttcacca atatataaag
     1201 aagtgcctat tttttcattt tcaaattcag caatttttaa tgtttcatat ttaggtgttt
     1261 gtctttctaa atatgcctga tttgttgcat cttcaattat tattgaagaa gggtcagaaa
     1321 ttggaagaaa tatacaaatt cttgtcccaa ttataatatt tctttcacca tttgctattt
     1381 ctacccattt ttctcctttc tcttttcttt tcatttctcc ataatataaa atacaatttt
     1441 taaattcagt atttaaaaaa gaaaaatatt cttttgctat ttcaacttct ggtaaaagaa
     1501 ataaaacaga gccatctttt atgttttctt ttattaaatt taagtaataa ttctttttct
     1561 catcttcctt tttaaaaata ataatttttc ttttgccttt ttcttttttt ccctttttct
     1621 cttttatatc aaaagaggta tgtctgatgg gtagtgtccc tattattgag aatattgcct
     1681 gaccaagtga agaaataaat gtctctgaaa taaaatttgc taattgatag agataagaat
     1741 ttattatagg acatttatca tatactttta ttactttttt aagttcttgt ttttcccctt
     1801 ttttaacact tacaacaaaa ccaacttttt tcgtttttcc aaagggaaca agaactctaa
     1861 caaaaggagc aatttcttta tcaattagat attcaaattt tttatcaaca ggaatgtcaa
     1921 agacaacttc aattatattc atttattttt tcttttagat gttttatata tgacgggtct
     1981 gttccacaac aaccacctat aaattccaca ggaaaatttt tacacataaa aaggcaattt
     2041 tctacaaatt cttctttatt ttcaggatat ataacttccc catgtagaag tttaggaatt
     2101 ccagcatttg gttttaccca taatgatttt tctgttaaag aatatatttt tttaacaatt
     2161 tctttcattt gctctgaacc aactccgcaa tttataccaa ttatttttac ttctatattt
     2221 cttgtccagc tgatataatc ctctattgtg tttcccataa ctgttctata ttctttccca
     2281 atctgaaagg agagacaggg aataattgta atttggaaat ttttctttaa aatagaatag
     2341 gcaatttcaa gttcagaaag gtctgtaaat gtttccagaa caattatatc aactttccct
     2401 ataaaaaatt ttgcctgaga taaaaaaatc tcttcacact cttcttgttt tatattccca
     2461 taagggtaaa gtaattcacc agttggtcct atatctcccg caactaaaat atttttttta
     2521 ttcttaactt ttgtggcaat ttcaatactc ttttcattta ttatttgatg tttatcttct
     2581 aatttttttt tacttaaacg ggctttattt gccccaaaag tatttgtcaa aattatttct
     2641 gcaccacttt ctacatattc agtatgtatt tcttctacaa ggtcatctct ttcaatacag
     2701 gcaagttcag gcgttagacc cttatagccc ttttttgtaa gatatgtccc tatcgctcca
     2761 tcaattaaag taattttctt atttcccatt tataattatt attccatata acttgcacat
     2821 tgtttttcat tttcccatac aattactttt tctaatttta taggtaaatt ctttaatagt
     2881 tccttcaatt tagtaaaaat atagtaagaa atattttcag aagttgggtt cgttttatta
     2941 aaaaagggaa tagtattcag gtttttgtgg tctaattgtt ttaaaataaa atctaatttt
     3001 tttttcaaga taccaaaatc aataagaagt cctgtattat caagagttgt cccacttaca
     3061 ataatttcaa ctttccaatt atgtccatgt aagtgttcac atttaccttt ataatttttt
     3121 aatctatgag cagatgaaaa tccccctatt acttttattt tatacattgg gaacattatg
     3181 gagagtatat tctccacttt ccgggaaaat tttcctaatg actttttcag tataaggact
     3241 tatatcacta aaaaaaagtt ttatttttcc tgtttgcttg ttatctttta ggttattact
     3301 ttctaaatat tttttaacat ttaatgcaac ctcatatgat gggtcaacaa tatttacatt
     3361 tctcccaatt gtctcttgaa taaccttttt caagtaagga taatgagtac agcccaatat
     3421 taaagtatca atattctttt tttttaacct ttttagataa tattctgcta taaaatatgt
     3481 acagggagca gtaaataacc cttcttctac aaggggaaca aataaagggc aggacattga
     3541 gaaaacggat atatttttat tatgtttttt gagaagtttt tcatattttc tacttaatat
     3601 agttgcagaa gttcctatta tacctactct ttttttccct gttatttcaa ttgttttttt
     3661 aattgctgag gaaacaacat caattatatt aagagaggaa aattttcttt ttaataattg
     3721 tccagcaaga gatgaagcag tatgacaggc aataataata acctttgggt taaattttaa
     3781 taaaaaattt acatcatcta aggcaaattt ttctataagt ttttttgatt ttgttccata
     3841 aggaactctt gcagtatctc caaaataaat aatgtcctca tatttcaaaa tatttttgac
     3901 ttttttaaca acacttaatc ctcctattcc tgaatcaaat attcctattg gatttttttt
     3961 cattattcaa ttatctttct tgatttaaga tattcaatta ttgcattacc aagtgactct
     4021 gcaattgcct gtcttacctc tgcatctttc aaatttaatt caatatttgg attacatata
     4081 aaaccaattt ctacaagaac tgaaaccatc ggagtgtatt ttaaaacata gtaatttcct
     4141 tctttcgctc ctctatcagg agttaaaaga cgttctgcaa gtttttcctg gacaaactct
     4201 gcaagaattt tactttcttc aattgtgttt gtggtattta attccagtaa aatatctgtc
     4261 aacattcctt cgtttgttgc ctcatcccgt aaaatccctt cgtcattaac tatttttggt
     4321 aaattttcaa ctctttgttt tgttcttgga taatatgtct caaaaccatt tgcatcaaac
     4381 ctgttatatt ttgctgagtt tgtatggaca caaaaaaaca tatctgcatt taaagtattt
     4441 gccatttgta ctctctcctc taatgttaaa aaaacatctt catctcttgt ttgatagacc
     4501 tttacattag aagatttacc aaagtatttg cggagataat ctaccagccg tgatgaaaca
     4561 tctaaattga catctttttc tctgagacca aaattcccta ttgcaccagt atcttttccc
     4621 ccatgtccag ggtcaattaa aataacatat gtattccctt tttggctttt ttcttccact
     4681 tgaattttcc tttgtggaat tggattttct tcctttgttt ctgggacttg agaaaaaata
     4741 gatgaaaaat tatccaatgg gacaagaacc tctccttcaa tttcttttat aggatatttt
     4801 agatttattg tcttatttcc aaatattact ttatcttccc caaccctgaa ttttatttct
     4861 tcacccttat aaattataaa aaccctatct tctatttttc cccaggattg tggctgtata
     4921 acatctatca gagaatttaa agaaatatat gactggtctt ttataatata tgttggtagt
     4981 tttctttctt taatagcatc atttaaattt aaagaaaata agttacaggt caataaaaca
     5041 aaaaatattg aaataatttt tatctttttc atttttattt attattttaa tttttttcct
     5101 tttataagtc aattaaatgt ataagttggg aaatggtatt tatgcaaatt aacaatttgt
     5161 ttttaaaaat ttttcaggga tgccaaaaac atatggctta ccagaaaatg ggtttttgtg
     5221 aacaataact tttgtttcat acgcctcttc aattgttttg tagtcaatta cttcttctac
     5281 tttcccttct tttaaaattt ttccgtcttt gaaaaggaaa atacatttac tatattcaga
     5341 agatatatta aggttatgaa ggattgtaat aattgttatt cctttttcat tatatttttc
     5401 aagaatatcc attattttga attcatgccc aatatcaaga tgagaaattg gttcatcaag
     5461 aataataact tccgtctttt gagcaaaaag ttgagcaaga aatacaaatt gtctttctcc
     5521 ttctgaaagt tcaaagagtt ttttgttgct taattttttt atacccaatt tttcaagaac
     5581 ttcttcaact atttcatagt cagccactct tattttctca aattttgaaa gatgtgggta
     5641 tctacccatt aaaacaaact caaaaacagt ataaggaaaa taaacattga tagaagatgg
     5701 caaaaaagaa acatcttttg aaagttgagg aagagatatt ttttttaatt cagttccttt
     5761 aaaaaaaaca ttacctttat aattttgaag tatttttgtt aaaattttga caaatgtgga
     5821 tttccccgaa ccattgggtc caattatacc aacaaaatct ccctttttta tagaaacatt
     5881 tatcccttta atcaccctgt ttttcccata agagaattct aaattctcca ttttataaat
     5941 ttcctctacc atattttttc acccttaaaa agtaaaaaaa gaaaaaacaa ccctccccaa
     6001 aaaccagtta ttaccccaac aggtatttca agtggtatta taataactct tgataatgta
     6061 tctgaaagta taaggaaagt tccacctaca aaaattgata aaggaagaag atgcttatga
     6121 agattgccaa aaataattct tacaatatga ggaattataa gtccaacaaa cccaattatt
     6181 ccagataaac taacacataa agcagtaatt aaagaagcaa tgaacaatat cttccttttc
     6241 tctttttcaa cttcaactcc taaggttata cttttttctt ctcctatact taaaatatcc
     6301 aatgttttat aaaaaacaat agagaaaaaa gaaagaacta aaattatagg agaaagaaat
     6361 tttataagag gaaaaggtgt tgacgaaagg tctcctagaa gataaacaaa tgtttgttga
     6421 aacttttcat ttttgaaaac agataagaga aataaaacaa gagaagaaaa aataaaattt
     6481 aaaacaaccc caagtaaaat taaagatgga tttgagaatt ttttaaaatt acttgcaaaa
     6541 agaacaattg aaatactcaa aatacctcca ataaatgcac aaaaaggtat aatcaaaaag
     6601 ttatcaaaaa ttactcctat acaaataccc aaacatgcgc cacctgatat tcctaatgta
     6661 taactttctg ccaaagggtt ttctaaaatt gcctgaaata taacacctga aatagaaagc
     6721 cctgcaccaa caacaaaaga tgtaataact ctcggcaatc ttattttaaa aagaattgtt
     6781 ctgtcaagtt ctgaaagaat agatggattg aaaattgaac ttattgaaaa tgattcactg
     6841 ccaattaaga cagaaaaaac aaatgaaaga ataaacaaca aaaaaacaaa cagaaatttt
     6901 attttagttt tcattatggg ctcattgtaa aattttagtg aataccattg gcaaggcaca
     6961 taggtataag tttttaaaag tgtaaaatgt aaaacgcaaa gtgtaaaacg acaatgtaaa
     7021 attgaaaatg aaatacaaaa aaagaacgtt tttaatttta aactttaaat tgtaatttta
     7081 aactttacac ttttaatttt aaatttatac atctctaaat cctccacaag acagacggcc
     7141 gctgaaaaac acactttgtg ccttttaact accttgctga gcatttataa atttatttat
     7201 tttaatacaa acatcaacaa tactttcagg tgttggactt gcaattttat attgttctat
     7261 ttccaatatt ttgttttctt taactgcctt tacactactt aattttttaa tatattggtc
     7321 aaaattttct cccatttcaa tataaattat aacatctggg tttcttttta ctacctcttc
     7381 tatatttatc tgaatccatt ttttttcaat agaagcaaat atattttccc ctcctgataa
     7441 cttaataatt tcgtttataa atgtattttt acctgttgtt ataagtggtt tccatccaag
     7501 aacaaaaaat acactttttc tttttttaca ttttaaattt ttaagtttct tttttgcttt
     7561 tttaacaatt tcttctcctt tttccttttt cccagttatt tctgaaagtt gtataaaatt
     7621 tttaaaaatg tcatttatag aaaaaagttc agaaagtact aatgttttga tacccaattg
     7681 atttaatttt aaaacacttt cttgcctatt tccttctttt gaagcaataa ctatatctgg
     7741 ttttaaaaaa acaattttct caatattggg ttgtaacata cttccaataa tttcttttcc
     7801 ctttttatat tcttcttcgt cctctaaagt caatccaata atattgttcc cttctcctaa
     7861 aagaataaga ttttcagtta cataagggca taaagaaact attcttaaat tttctgttat
     7921 agcaaaaaaa gaaaaaagaa acaaaattaa aattattttt ttcattctaa atcataaagt
     7981 tctgatttta ggtaagcaat tgaagaatca taagaactta tattatattt atcttttatt
     8041 gcttctacat gtccatcaac aaaggcaaca tttgcaaatc cattatgtct gaaatggact
     8101 gttggaccaa aatcataagg gtcttccttt atccttaaat aattatttcc tataatttca
     8161 tttgtataag ttgaccatat tccactatca gcaagaagta ttgtctctga tggattttta
     8221 attctgctta actttgttgg tgggtctgac tgtcctgtcc agatagaata tcctccacct
     8281 atgtatgttg aattataagc aaaaccagta aatggtctat cgtatgattt tattattttt
     8341 tttgcagttg gacattcaaa tattccttta gttaggtaac ttccaataat ccctttttca
     8401 taagttgccc accaagtatc tgttttaaaa tcccaagcaa tttcacttgt gtatgtaata
     8461 taataacttg gtggaaaata ttcatcatta tcttgaacat acattaagaa cgcaagagta
     8521 agttgtttta aattactcag gcatacagat tgtcttgact tttctcttgc ttttgaaagt
     8581 gcaggaagaa gcattgatga tagtatcgcg attatcgcta ttactacaag caactcaata
     8641 agagtgaacc cgtaagaaaa ttttttgtgt cccataaaat ccttctcttt tttgtaggca
     8701 ggaaataaaa aacccgccaa ttgtggcggg ttgcaccctt attcttcacc ctttttattc
     8761 tgtctgccgc agaataaaaa ttaccacttt cagggcaggt cttctgactt gaacttttac
     8821 ctactctctc gtcttcccat ccgccaaagg cagacagtga cttatgagat ttcgtcagtt
     8881 cttacagcgc cgggagcgtg tgagattttc acccacttcc ctattaagca cactttgtgc
     8941 accctgaatt ttttatgtaa ttttaaaacc agttattatc tttataatat tgatattctt
     9001 ctatccagtc atctttccta ttttcaatta tttttctaaa atattcttta tgactattga
     9061 ttttttgtat ttgctcagga aactcctttt tttcttttat tgcatccaca atggcctttc
     9121 ctaattcatt tgcatgtttt tttacttctt ctgttattgg aatttttgta aaaacaccac
     9181 caacatactg tgccccacag gaaattgaat attcttttat gtaatcacct atcatatcct
     9241 gaggtccacc accagatgta gcaacaactg ctgtatattt gcccataagc cgatgacaat
     9301 ggagaaaatg acttgaccta tcaagaaatg tttttaaata tcctgttata tgatttagat
     9361 atacgggact gccaaaaata ataccatcat tttccagaat tttattaagt aaaaaagaag
     9421 catcatcttt tataggacat gccattattt tcttatgaca attttcacaa gccctgcaaa
     9481 attcaattct aaaattacat aaatcaatat attccgtttc tgcacctttt tgttctgcac
     9541 ctgataaaat atctttaagc aaaatagatg tattacttat ttctctacga ggtgatgcag
     9601 aaatacctaa aatctttatt ttttcattat tcatattctc tatattacca cctcattttt
     9661 cattatcaag atataaatcc cgataccaga tgtttctaaa cctcacagga ttactatggt
     9721 cctgaagcat taaaggtcct ttgtttggat gtggtttgta aggcaatatt tgtttatgtg
     9781 cagttggacc taaaatttcc cgatgataat ggacaacaat tccattatga aataatgtaa
     9841 taatagcagg acttataagt ttctctcctt taaatcttgg acagagccac actatatcat
     9901 atgtctgcca ttctcctggc tttctacagg catttacaag tggaggaaat tgtccataaa
     9961 ttgcagcagc agaaccatcg gcatatgtag gattgttata actatctaaa acctgaattt
    10021 cataaattcc catcataaaa acaccactat ttcctctccc ctgcccttca ccttctatct
    10081 tttcaggaga tgcccattct atatgtaatt gacaatcccc aaattcttct ttggttttta
    10141 tattccccgt ctgtggaaca acttccatat aaccattttc tactttccat tctgcctttc
    10201 taccatctaa gtgaacccat ccacaaagac attttccatc aaaaagaatt tttgcatctg
    10261 aaggtggaat acctgccttt tcctgagtac ctggtgttcc tggggccacc actggtggat
    10321 atggtctatt cctatcgtgg actttatact ttgaaccagg aattaaaggc gtatcatcat
    10381 aaccaggcct ttctttttcc atttttttct cctccccctt tttttatttt attttttcat
    10441 cggatataaa ggcagttctt ttacaaacag gacatataat tttcattata tctgttcttt
    10501 gtattggtag aataactttt tcttgttctt ctgtctttat attcaaattt tttataatgt
    10561 ttgtcacaag tacagcgttt gaataagata gattccctaa ctttttatat atccatataa
    10621 tatgtttctc tgtccctatt atactaccac aacttttaca tattacaaga ggatattcaa
    10681 ctttattttc aacttcttca ggttctttaa aatatgaaac atcaaattta tttgtaagtt
    10741 ttatcccttt tgtcgttata caatttcttt cgcattctcc acagaaaaga catttatcag
    10801 gatggtgaat aagaactctt ttttcttcac caatatcatc aattacttca attgcttttg
    10861 aaggacatac atttgcacat gcaccacaac caacacactc atctaaatta aattcaggtc
    10921 tacctctaaa tcttttcggt ggaatatgtg gctcataagg aaattttgtt gtgtatggcg
    10981 gtgtaaataa acttattatt ccttctttca attctcttaa ttttggtttt ttcatttttc
    11041 ccacctattt aagttttgtt tttatcattt gttggaaaag aaatttcaaa taccttaaat
    11101 acctttttta accagggaac tatttttttt aagaaaatat tataaatatc aaggtatcct
    11161 ttatcttccc aattgtaaat tgtttttaaa acaggtaatt ctcttaaagt caaataaaat
    11221 tgagacccat caaaagatgc aatttctctt tcaagttttt ctccacctgt aaatatttct
    11281 tttttactca ttttcccaat tattttttta taaaaaatga aaattacaaa aaataccagg
    11341 actaaaagga taccgtagaa attcacaata ttgataagag atatatttac ttctggggca
    11401 aaaacaattt ttgaaaaaac attattcata aagaaatcag gaaaaacccc taaaatgaaa
    11461 caaagaacag ataaaactcc cattgggata gaaaagaaag gtgaaattct actttctttc
    11521 tgtatatttt ctctttttct tactcccagg aatgtatcat ttaaaacttt taaacaggca
    11581 gccagagtta aacaacttcc caatattgca attaaaaacg caaccgttgt aagaactgtt
    11641 cctttttcaa gtaatgcttg ataaataatc cattttgaaa taaatccatt aaaaggaggt
    11701 atccctgaaa ttgaaagaga agcaataaaa aatgcaaaaa atgtaaatgg cattttttct
    11761 ccaagtccac cataatcaga taaatttcga gaaccaactt tttttattat tacccctgct
    11821 gcaagaaaca atagtccttt aaataaagca tgatttaatg tatgatacaa tgcacctaat
    11881 agtcctaaat ttgttccaca tgcaattcca aggaatatat aaccaatttg tcctatactg
    11941 ctaaatgcaa gtaatctttt tatatcattt tgaattattg caagtaaaca accagtaagt
    12001 actgttaaac aaccaattat tatcataatt gtttgccatc caatagacca tgaaggagag
    12061 aaaatttcaa aataaaatag tattaaggca taaattccta tttttatcat aattcctgaa
    12121 agtaaagcag atattggagt tggagcagca ggatgagcat cggcaagcca actatgtaaa
    12181 gggaaaagac ctgcttttat tccagcacca attataattg aaaagaaaag gagatgtttc
    12241 cagaaaatac tttgtccagc aattcctgat aaataaccaa gtattccaaa aaggagaaat
    12301 atacttcctg ctcctgtcat aataagatat tttatagagg cactttttgc ttcttttgta
    12361 ttctcataaa gaataagaaa aaaacttgaa aaagtcataa attcccagaa agaataaaat
    12421 aaaattaaat ttggcgacag agtagttcct aacattgaac caataaagaa aaataaaaga
    12481 caatcataaa caaaagttga ttgccaatta tatttctgac tataaattat tactaataga
    12541 ccaagaaaag aaataagaat agcaaaaaaa ctgcttattc ctccaagtaa taaaaaccat
    12601 gtgcaattac aaactatata tattgtagat aacaaaacaa ccaatccacc taaaatagaa
    12661 atccagcatc tcttttttct acttaactta taaaataaaa tatgaataag tgaaattata
    12721 aaacatattg cagttggcaa taaaattaaa gtttctttat tcaaataatt cctcccctgt
    12781 taataaagtt aaaataatac cgcctaataa tgacaaaata cataagaaaa gcacacaaaa
    12841 catcatgaac ttatattttc tttcattgtt ttctgtaatt atgtctgtct gttcagttgt
    12901 tcttataaaa atatatttaa ataaccttaa aatataaaaa agagtaagtg ccagagttat
    12961 aattgaaaag aaagcaaaat aaatatttcc tgactttaac attccataaa tgatattcaa
    13021 tttaccagat gaaccaagaa aaggtggaat accaattatt gaaagtgatg aaaacaaaaa
    13081 ggcagtaaat gtgaccggca ttttttttgc acttacagaa atttttgtaa tatcatttgt
    13141 tttaaatacc ttttctaaaa ttcctgcaca cataaacaaa caccctttac caagaacatt
    13201 tgctacataa aaaaccataa tacctttaaa tgcaatttca ttaaaagaaa gaagtccaca
    13261 aatcataaat ccaatctggc ttatagttga atatgtaaga atttttttta tatctatttc
    13321 tataaaagat aatactccac aaagtacact gctgaataaa gcaatatatc caatccatga
    13381 aacactggac gttataaaaa gtgtctttat aaataatttt gcaatcccat aaagagatat
    13441 gttaaccaat atagtgttaa agaaagatac cacaggagtt ggtgcaattg ttgttgaagg
    13501 gatgtagtaa tgaaatggaa aagttgctga ttttatcatt attccaataa aaaagaggta
    13561 aaaaacataa ggaggaatac aactcccttt catttctagt aaacttaatg agtttgtatt
    13621 tatataaatt aaaataaaag agaataatat tattattgct gaacaaactt ttgtaactag
    13681 atatatattg tttttgctgg aattttcatt ttctccttta taaaatgcaa ttaatttata
    13741 aaaacaaaat tctgctaatt cccagaatat aaaaagtaaa aataaattgt cagaaaatac
    13801 cacacctgtt agacatccaa ttaaaattaa actccagaaa gaaaaattat ttttaaactc
    13861 ttcattttca tctgaaaaat aatcaataga ataaagaaaa ataaaaattc caattataac
    13921 agaagttatt gcaaaaatat aacttaaaaa atcgcatgaa aatttaatac catataaaaa
    13981 ataaacagtt gtcccagttt tggaaattag taaaagagaa ataaattgta ataaagtaaa
    14041 gaaagtagca acaattattg gttttctctt taaaaatgtt tttatacaaa aagcgcctaa
    14101 aaatggtaat accaatgcca gaattatatt attcattttc tcttaatttt tttgtttttt
    14161 ctattgataa cttaacaagg tcttgaaaat cataaagttt tttattcctt ttttcaatat
    14221 cataaacagc aaccctttct gtgcaacaat aacaggggtc aactcctgca agaattatac
    14281 aaacatctga aattgtctgg ttaatacaac ttgttttaaa tgttggaata tttgtataac
    14341 tcggtgctct tattttatgt cttacagggc tattacttcc atcacttctt atataatgaa
    14401 aacactctcc tcttggtgct tcatgatgtc ctattccttc tccttctggt atttctctta
    14461 cttccttaca tatttcccct tttttccctt ttattgcttc taacgattgt tcaattattt
    14521 ttatactttc aaaacattca cctaaccgaa cgactgcttt atcaaaaaca tctccatttt
    14581 cacatgttat aactttccat tcaactaaat tataaccagc ataggggtca tcttttctta
    14641 catctatatc aattccagac gctcttgcaa taggtcctac tgctccatag ttttttatat
    14701 cctcttttgt taaaactccc actcctttaa gtcgtgctgc taaaactggg tcatctaaaa
    14761 ctgcctttgt taacattcca actttctttt ttgtcttatc aagtgttttt ttcaataaag
    14821 gtatatcctc atcttcaata tctcttctta ccccccctat tttaagcatt gcataatgat
    14881 ttctattacc tgttattgtt tcaaacatat caaggattgg ttctctatat ttccacgccc
    14941 acataaaaac agtattataa ccaagaaaat gtcccgcaag tccaagccat aaaagatgac
    15001 tgtgaagtcg ttctagttct ccaattatat ttcttatata ttttgccctt tcaggaatat
    15061 ctatttccag taaattttca actgcattac aaaaagcaat tggatgggat gttgaacaaa
    15121 taccacatat tctttcaaca agaaaagtta cctggtccca atgtttactt tcagaaagtt
    15181 tttcaattgc tctgtgatta taaccagcaa cccattcaac atcaacaact ttttcacctt
    15241 cacaatataa attaaaatat tctggttcct ctaaaagagg atgatacgga ccaattggaa
    15301 caacaacttt ttttctatta tttttttcca tctgttttta cctctttcct taagggataa
    15361 ttaccttctt cccaatcatc tcttaaaaga aaatgtttta agtcagggtg tccaataaaa
    15421 tttattccaa gaagttccca tatttctctt tcaatataag aaataccagg aattaaacaa
    15481 gttaaacttt caatctctgg ccttttttta tcatcaataa atgtttttaa agaaacaata
    15541 acaccacctt ttttatcaaa agaaaaatga tataaaagtt caaagttatc aaaattatca
    15601 ataccagtta caataatata cctaccttcc atatatttaa acataatttc tgttgctttt
    15661 ttaatatctt ttttatcaat tgttatatat attctttttt cattaactac ctttatttcc
    15721 ttgattaaat tgcctaactt ttcttttaat atatctttca ttttaatttc tccagaaatt
    15781 taactattgc tgatatcatt gcctctggct ttggtggaca ccctggtata taaatatcaa
    15841 caggaataac attattataa ggacctgcca tttgatagcc atctctgaaa actccacctg
    15901 aacaagcaca tgcaccaatt gccactacaa cacatggctt tgctgcctgt tcataaattc
    15961 cttttactct ttcaaaacat tttttattta taatccctgt gcataaaaga acatctgcat
    16021 gtcttggaga accaaccaat accataccaa atctttcaat atcaaacctt ggagtaagaa
    16081 ggtcaagaat ttcaatatca cagttattac atgaccctcc acttacatga tatacccaca
    16141 aacttttctt caatgctttt aattttatgt tcatattacc atcctaaaaa agataaaatt
    16201 attccaataa ttcccaaaaa agtgagatgt ccccaaaaaa atctcattgc ctggtctatt
    16261 cttaatcttg gatttgtatt ttttataaga acaatcaaaa agacaattaa aatatatttt
    16321 aatagagata ttataatagc aataggcgaa gagtaagaga aaccaccaag aaaaagagtc
    16381 ataataaaaa caggtccaac aataagtaaa acatattttg taagtttaaa aagtcctaat
    16441 aaaattccag aatattccat aaaaacgccc gaacttattt ctgtttctgc ttctgctgta
    16501 tcaaaaggaa caattccaat tttacctgtt ataacaaaaa gagaaattaa aaaaccaata
    16561 aatccagata atgaaccaat aaaaatatgg gtctgttgtt gaacatttaa aattgttaaa
    16621 agtgaagtag ttccacctgc tttgatagca gaaattataa ttgcacatat aaatggtaac
    16681 tcatagccca aaagaagttt taattctctg cttaaaccaa ctgctgataa aacatttcca
    16741 gatgcacttg ctcctaaaat tattgcaaga gatggaattg ttaaaaaata aacaaaaaga
    16801 ataatgtccc ccggcaatgt aaaaatccct ttctgaacaa gaatagccat aactccacaa
    16861 ataattactg atataaaaga tataattgga gataaaataa aaataggagt atttgctcct
    16921 tctggaataa ttacttcttt tcccattaat ttaacaaaat cagcaaaatt ctgataccat
    16981 gggggaccta ctctaaactg gattcttgca gtaagttttc tttcaatcca atttgcaatg
    17041 catccaccaa ttgaaagaaa aataccccct aaaataaaat accataaata ataagtaaat
    17101 gtcatttttc tattccctgt aatgaaagat aattataaac attaacaata agtattttcc
    17161 ctacttcaaa agtaatcttt gttttctcaa tatctttttc atcaacaaac ttaattgctc
    17221 cacaattaca actttctaca caaacaggaa tttcgttttc ttttaatcta tttatacaaa
    17281 aatcacattt tgaagttaag taacttacaa tatcaggcac aattgtccca aatggacagg
    17341 caattaaaca acttttacag gaaacacaaa ggatattact tcttttaaca actccatttt
    17401 ctctttttag tgcctggtta ggacatgcat ttacacaggg ataatcctcg cattttctac
    17461 atgcaagaag aaacgcaatt tgttctttta agttaataat tccattattt tcaggatgta
    17521 atatataact acattttaac tgacatttac aaataagaag tttatcataa tcaataaata
    17581 attttttcat ctcacaccca tatatttggt ttattcttta tctggtcata agtaaaaaca
    17641 ggaccatctt tacatacata aaggtcacca attaaacagt gtctacaatg tccaactcca
    17701 caatacattt ttctttccat agaaagatag atattactat cagaaaaatt tatctgtaat
    17761 aatttcaaag ttgcaaattt catcataatt ggtggaccac aaacaacagc aacagaattt
    17821 ttaatatcca tatcaagagc atcaagggta acagttacaa ccccttccat atatttccat
    17881 gtttcgtctt ttttatcaac agttattcta aatgataagg gaattttttc acaaagtttt
    17941 tgccaatcac caaataattt gtctttatat atataatcag aaggactttt tgctccacag
    18001 cagaaaacaa tttttttata attctgatat gtatgaacaa gtgctaaaaa caatgctctc
    18061 acaggcgcaa gtccaactcc tcctccaaca agcaagacct cttttccttt aaatttttct
    18121 aatggatatg gtttcccata aggtcctctt accccaacta tatctccttt ttttaattga
    18181 tgtatttttt ctgtcactct ccctgttttc attactgtaa attcaagttt ttctgtatca
    18241 aaaggagaag aagaaggagt aaatggtgct tctccaactc ctggaatagt taaagaagca
    18301 aattgtccag caagaaaatt aaattgtttc tctggcttta aaacaattgt cttaattgtt
    18361 ggagattctt caattacatc aattacttca cattttattg gtttataaat gttttctgtc
    18421 atttaactac ctctttcacc agttcactta taactcttct tttatcaatt ttcccaatac
    18481 atccacttat acatcttcca caaccagtac accctaaaat attaaaattt gagaccatat
    18541 agtcatattt gcataaaaga cgatttgcat acctactatg taaattaggt cttggagttg
    18601 caccagaagc cattcttgca tatccaggga aaagacaact atcccagaat ttgatttttt
    18661 caaagttctc tttttcactt gtatcttcaa gaaaaaaaca tacacaactt ggacagttaa
    18721 aattacaact tccacaggaa acacaatttt ttatatcatt aattttttcc catttttcag
    18781 tttcatatac ccctttaatt ttttcctgaa aattattaag attaaatacc tcatttattt
    18841 cttttattct ctttactgtt ttttctctca gaacatttct attgtcaatt tgctgcacgg
    18901 ttgcattaaa aaacctatta tcttttccaa taaattcatt acctttttca gttgcaattt
    18961 caacaataaa accatctgtt gtcattgaaa aattaatatc aaaatttttt tcaggatatg
    19021 gattggtccc aacaatttca caaaaacagg tatctgatgg ttgataacag tcagtactaa
    19081 ttataagcaa attttctctt cttcttttgt aatttgggtc cttatattca ccattagcaa
    19141 aaacattatc aagaatttca agtcctttta aatcacagga agcaattcct attaaaataa
    19201 tttcatcaaa gttttctatc tcattaacaa ttttttcaac aataggaagt aagaaaattt
    19261 taagtggttc aactgttctg aatttgttat agacaatatt ttcaacattg ttttcatcaa
    19321 taagatttaa aaataaattc tgattttctt caattggagc ataaactttt ttttctttaa
    19381 tttttccttt taaaaaattt tcacagtcct gttttgtgat aaataatgtt tccacttgtt
    19441 cctcttttca caaagttata taaaaaaata aacaaacttt ttaaattaaa acataataaa
    19501 attttttgtc aagcattttt tattatacca ataattttta attaagcaac aaaacaattc
    19561 taactctaaa attctgacat aaggaaattt tttatttaat attcaaaaaa tctattccct
    19621 ctttcatttt ttaaaattaa ggaaaaagag gtaaaatata taa
//
LOCUS       DAOOVW010000031        18595 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_2034_length_18595_cov_8.251888, whole genome shotgun sequence.
ACCESSION   DAOOVW010000031 DAOOVW010000000
VERSION     DAOOVW010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 18595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18595
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2034_length_18595_cov_8.251888"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PLW95_05480"
     CDS             complement(<1..255)
                     /locus_tag="PLW95_05480"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOV22114.1"
                     /translation="MFHLSSYTISQIYKTRWEIEIFFKWIKQNLKIKTFLGTTKNAVL
                     TQIWTAMIYYLLLSKYQTKYKYSLLYMTRVIKKCLFKRADI"
     gene            642..1595
                     /locus_tag="PLW95_05485"
     CDS             642..1595
                     /locus_tag="PLW95_05485"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOV22115.1"
                     /translation="MVKVCIIGAGGEANLVHYPSLTKMENVEIVGICDLNQERLRQTS
                     EKYKISNIYTDYKKMLEEKNPDAVYIIVPPHYLYDIVMPCLDRGFNIFTEKPLGMTAE
                     QARQMAKLAERKGVLTMVGFNRRFAPLIVEGKKRIKERGEVVLAEARFFQNYLDRPLD
                     YNGAIDVLTYFVIHSVDLLRYIGGEVKQVKSMVKGVFADYNNIFCALFEFSSGAIGTL
                     SADWASGKRVCSIAIHGKGIAAFIEHEKQAIIYSDGKEDGECIDVKEFTGKKEFFEIA
                     GFLDEHKYFIECMEKKELPETNFSDAVKTMELIERIYRNTI"
     gene            1653..2813
                     /locus_tag="PLW95_05490"
     CDS             1653..2813
                     /locus_tag="PLW95_05490"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOV22116.1"
                     /translation="MDRIKIGVIGVGVMGYAHCLSVKEIEESELTCVSDAAEGLARKR
                     GEEFGVKYFTDYKELITSGLCDAVIVATPHWFHPEVSIFAFENGLHVLSEKPIAVTVS
                     AADKMIESAEKNNRVFAVMFQKRTKYFVRKAKEIVDSGKIGEIIRTVCIDPWYRTQEY
                     YDKDIWRGTWVGEGGGVLLTQAPHMIDIFTHLAGLPCKIEAKTRTKLHNIEVEDEVSA
                     FLEYQNGAWGYYYTSTCEPIGYYYNWEPVGPFYLEVSGDKGKIVIRGDQIAFYNYEAP
                     LYETSMEEFTKKIPNIWELSTTPLKLEEGQIVPTSNFQTGHKEIIRNFVRSILYNEKL
                     ITPGKEGLKTIEFMNACLLSGKKNKVVNIPVDRKEYDDLMEELKKTSKAKNL"
     gene            2910..3890
                     /locus_tag="PLW95_05495"
     CDS             2910..3890
                     /locus_tag="PLW95_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOV22117.1"
                     /translation="MKKIRIGQIGMSHEHASAKMMTLRQLTDYYEVVGIVDDLNYNAP
                     KYPGMDMECYAGIRRMSEEELFNVKGLQAVAVETANTELVPTAIRCMKRGLHMHLDKP
                     GGEIMEPFTELIEGCKRKNLAIQLGYMYRVNPAIKFCFKAVREGWLGEIFEIHAIMNR
                     FDDENYRKYLSNFKGGAMFNFGCHLIDLVISIMGRPENVVSFQKSTADDGLNDNGLAV
                     LEYSRATATVNSAITEVDGFKYRRFIVCGTKGTVEICPLEHSADLYRIEPLYARLTLL
                     EDNSEYSAGTHKVNVGVMNGRYENQLIELAHIINGEISNPYTYEHELLVQ"
     gene            3959..5140
                     /locus_tag="PLW95_05500"
     CDS             3959..5140
                     /locus_tag="PLW95_05500"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOV22118.1"
                     /translation="MKKIQKSKRTILNVPSVKKWRIGVVGYYSCSGLGGHGLHLAFSG
                     LPGVEIVSVADPDETGRNMLQKETGAKNSYSDWSYLIQHDKPDVVCVCSRLPSQHTDV
                     VVAAAEAGCHIYCEKPFAQTLEDADKMINAAASNNVLIVVAHLGRYAPMYQVAREMIQ
                     KGDIGRPLSVYCRGKEDERGGGEDMMVLGTHLFDMSCFFFGYPEWIFGNITVKGRKMT
                     LNDVTEPKEPIGPVAGDEIVSLFGFSNSVRGYFESRRGLFDKKIRRMGISVAGSEAIL
                     AMRFDAKPRLYISTDRRPFEKPGDFDEIDIPSVLEIPGTQPLSLSVSDPENDMPFTYC
                     NRYAAVDLLCAIVEGREPISSGKDARWALEMIFGVYTSHLAGKSVDFPLPNRKHPLTR
                     K"
     gene            5183..5929
                     /locus_tag="PLW95_05505"
     CDS             5183..5929
                     /locus_tag="PLW95_05505"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOV22119.1"
                     /translation="MKLKDEVAIVTGAAGYIGSTTAKKLASEGANIVVCDMNIDGSQR
                     VVEDIISKGGKATAIQLDVTNTDSIENMVKCVIEKYGHIDILINVAGGSERGRNAPVH
                     NLKEEVIRETIAVNLLGVIFCSRAVVGYMIERKKGKIVSVASVLGLRGKRNFSSYSAA
                     KAGVIVFSKTLAMEVGPYNINVNCVSPGNIPRPGEPNAHIPTKNYFGRIPTADCVANL
                     IYFLVSDEADFITGQNYIVDGGETLGLKGA"
     gene            6016..6975
                     /locus_tag="PLW95_05510"
     CDS             6016..6975
                     /locus_tag="PLW95_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HOV22120.1"
                     /translation="MRAILVDKNKELVCKDIPEPEVKDNEVLIEVHAIGINRADLMQR
                     DGEYPSPAGWPDIMGLEVAGIVLSAPSQSRWKKGDKVCALLGGGGYAEKVSVPVNMVL
                     PVPEGLSMAQASAIPEIFATSWLNLCIEGKMKHGETVLIQSGASGVGTAAIQIVKAFG
                     GKVITTVGSDKKVEFVRSIGADIIVNREKEDLLSVLEKYPVDISLDCIAGPSLGKCIE
                     KMAQGGRWIVIATLGGIMTEINMNIFFKKGIRIIGSTLRNKSTEMKGKILADMESALW
                     PKFSSGDIKPVIYKILPIDQVSEAHAILERRENLGKVVLTISE"
     gene            complement(7086..9677)
                     /gene="clpB"
                     /locus_tag="PLW95_05515"
     CDS             complement(7086..9677)
                     /gene="clpB"
                     /locus_tag="PLW95_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOV22121.1"
                     /translation="MDLEKFTIKAQEAIMNSQAVAQERGHQAIDTLHLLYSLVDGDET
                     TAEILKQMNVKVENLKEDIERELKKIPEVFSSQTSVYITPSLNQVFYIAGKQAKTLGD
                     EYIGVEHLLISLSEVDSPAKTILKKYDIFSQRIIKIIDNLRGGEKIKDKDAESKYNVL
                     EKYTKDLTEIARKGKLDPVIGRDEEIRRVIQVLLRRTKNNPVLIGEAGVGKTAIVEGL
                     ARRIVSGDVPENLKNKRILSLDLGLLVAGTKYRGEFEDRLKALLKDIEKREDEIILFI
                     DELHTLVGAGAAEGAIDASNMLKPALARGLLRCIGATTLDEYRKYIEKDRALERRFQP
                     IFVKEPSVEETISILRGLKERYEVHHGVKITDSAIVSAAVLSHRYITDRFLPDKAIDL
                     IDEAASKLRMEIDSMPEVLDEIKRKMIQLEIEKQALKKETGEEIKERLEKIEKELEKL
                     KKEREKYNKQWLAEKEVVEKIRNIKQQMEVARGIVEKAEREGDLDKAAEYKYGKLIEL
                     QKQLEKETEKLKEIQKKGSGLVKDEVTEEDIAEIVSKWTGIPVSKMLEGEKEKLLQME
                     SRLKKRVVGQDHAIEAVSNAIRRSRAGLSDTNRPIGSFIFLGPTGVGKTELAKALAEF
                     LFDDENAMVRIDMSEYMEKHSVSRLIGAPPGYVGYEEGGQLTEKIRRRPYSVILLDEI
                     EKAHQDVFNVLLQLLDDGRLTDGQGRTVDFRNTVIIMTSNLGSQYITMFKNEDAMKEK
                     IMEVVKSNFRPEFLNRIDEIIIFNKLKKEDIIKIVDIQLSYLQKRLEEKKIELTATDK
                     AKEYIAEKGYDETFGARPLKRLIQREIENVLAGKILSGEFKEGDNVVIDCRNNQLAFS
                     LKSPVAS"
     gene            9881..10642
                     /locus_tag="PLW95_05520"
     CDS             9881..10642
                     /locus_tag="PLW95_05520"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOV22122.1"
                     /translation="MNNEFKTKIVGEVICGYRVIDKIWQGATSTVYKGIKLNNSYNSV
                     IAIKILHSYRNSKQYLKQFIKEYKIQKSLNHPNIVKVFGFGKQENLYCIFMEYIEGKT
                     LRLTYDENLIQEKNLFHIIIQIGKGISYIHSKNIIHNDIKPENIIVEKSCRWAKLTDF
                     GYAVKKSFFRRNISIQGGTENYIAPERKKGISNFKTDIYSYGMIIEELLFEKLPSEEL
                     YDIVAKTTDKVPSQRDENVDDIVNQLEKIYKRIYK"
     gene            10639..11376
                     /locus_tag="PLW95_05525"
     CDS             10639..11376
                     /locus_tag="PLW95_05525"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOV22123.1"
                     /translation="MKFSIISDIHSNYEGLTEILKYILTEKTDYLFVCGDIIGYGPDP
                     ELCLEKIFSITDLIVKGNHEEGIITGNYSRFKKLAKISIEWTEKKITNYIEKIKCLPV
                     KITFGDITFVHACLSDPIYKYILSKKDAEREFELFSNKICFYGHTHIPSAYKKNLKTN
                     NVETIIPDFNGKMCFKIEDDYKYFINVGSSGQPRDGLPMVCISIYDSESKIFKLSRLN
                     YPAEITKNKILERGLPSSLGEKFLKGI"
     gene            complement(11378..11830)
                     /locus_tag="PLW95_05530"
     CDS             complement(11378..11830)
                     /locus_tag="PLW95_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22124.1"
                     /translation="MKKVLVVLCLFLFSISGYSATKKATIDEVKTSIDEVEKVIKTGN
                     EVLVGIYSSALEIEKRATTDYMAEIIKNKICKITKISSNDFDKLREKYSFFDLAIGCA
                     VSQVNGIQIDKLMKTKENKGWEDILSLNGNCEKVKDIITKINQLNPKK"
     gene            11901..12422
                     /gene="pyrR"
                     /locus_tag="PLW95_05535"
     CDS             11901..12422
                     /gene="pyrR"
                     /locus_tag="PLW95_05535"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012449150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOV22125.1"
                     /translation="MERKIMDEKQMREKLIFLSKKIKNDKQLSDFVIIGIKRRGAVLA
                     DRIKKLIGKENLDVGYLDISLYRDDLSKIGPNPIISQTDILFSIDDRKVLLVDDVLYT
                     GRTVRAALDAIFDFGRPSLIKLLVFIDRGHKELPICADYVGEEVKTKKSDIVEVKVKE
                     IDGVDEVVIVERK"
     gene            12419..13342
                     /locus_tag="PLW95_05540"
     CDS             12419..13342
                     /locus_tag="PLW95_05540"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOV22126.1"
                     /translation="MTWKRKDLIGLEDLSRYEIETILETANSFKEVSSRSIKKVPALR
                     GKTVVNLFFEPSTRTRTSFELAAKRLSADVLSFDVSTSSISKGETIVDTAKNIEAMKI
                     DCFIVRHSVAGASELISRNVRSSVINAGDGCHEHPTQGLLDIFTVKDVFGKIEGIKIA
                     IIGDILHSRVARSNIWGFTKLKSKVIVCGPPPLIPSGIERTGVKVTYNFKEAIENSDI
                     VYLLRLQKERQKENFLPSLKEYSLTYGLNEERIKGINKNIFIMHPGPINRGIEIKSET
                     ADRENSLILQQVTNGIAVRMAVLYLTIGSQK"
     gene            13339..15246
                     /locus_tag="PLW95_05545"
     CDS             13339..15246
                     /locus_tag="PLW95_05545"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOV22127.1"
                     /translation="MKIPTYPAIDYKNSLNERQYEIIKKADGHSIVIAGAGSGKTRVL
                     VYRVCYLLDKGIPPASIMLVTFTNKAANEMIERVKEIVGVYPKGLWAGTFHHIGNILL
                     RIYGKNINIPSNFTIMDEEDSISLIKEIIDKLPNKDDYPYPGKIKNFLSLSVNTGQPI
                     RDIIDTRFIEYSNLIPQIERISKDYTKKKKDFNLLDYDDILLFWYRLMKDEKVGIQIS
                     EKFLYIIVDEYHDTNKLQAQILYQVAKVHRNIMVVGDDAQSIYSFRGATVDNILEFPK
                     IYPDTKIFYLDVNYRSTPEILDFANNIISHNKNQFPKNLKSIRKRGVKPLIIKCYDIK
                     EESIFVSRRIFQLLQSGVIPSDIAVLFRSRYQSAELEIELNKLKIPYVIRGGLRFFEQ
                     AHIKDIICFYKVLINFKDEISWNRIFFLTEGIGKKGAEELIKVIRESDEIEVLEKKIE
                     DIKINSKGLKNFKNQLGLIKKIKNLKISEGIDLILISFYSEYLKKKYKDDVERKQDIE
                     ALKEIADMYSSLTDFLSEATLHEHTKGEIRQKGNYIVLSTIHQAKGLEWKFVFIIGVC
                     DNHFPYISAKNDITALEEERRIFYVGVTRAKEDLYITYYIRDFYRNYYYNKSIFIEEI
                     PPYLYESWDFQ"
     gene            15262..17169
                     /locus_tag="PLW95_05550"
     CDS             15262..17169
                     /locus_tag="PLW95_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22128.1"
                     /translation="MEKNIFTKRSILIGTILSVFISVSDVYNLMVIQGSDMTIDFTTG
                     AAIYLIFWITLFNYLLKKISPKFSLSIAEMILIYIMMIVSCSIPTMGLTLYLIPLIAG
                     TKYYANDQNNWADLFIKHLKNWTILNDNNAINWFFEGIPKGQSIPWAPWIKVLILWIP
                     LIIALYLAMIFIMVILRKQWVENEKLNFPLTKAPISLIKADENKIFNNNLFWLGFAIP
                     FIIGCINGLHFYNELFPRIQLATSTPIFRRTMSLPYRISFPMIGFTYFVPLPLSFSLW
                     FFCLLSTIQQGFFNITGFGISEFLPYNAERPILGWQSLGSLFIIVIYGLWIARKQIKD
                     VFRKGIGKEKDDSSEYEIVSYKFAFWGLVISILVIFWWIVLSGLPPFPSIFFIIFMFI
                     IFIGITRAICEGGISTTRAPVIAPVITTSFFGSKNLGPSALGPLGLTFVYSSDVRTFV
                     MASVANGLKILEEVKGKKKIIFGAILISIVTTLISSIWTVIYLGYKYGGINANAWFFV
                     NGPQYGWQYIVQELKTPSGPDMIYWGFFAIGAVFTIFLQFMRMRFLWFPFHPLGFAFS
                     TIMLTNELWFSIFLSWLFKFMILRYGGANVYEKGKTFFIGLIVGQFIVSGTWLFIDFF
                     TGRTGNILFWI"
     gene            17172..18467
                     /locus_tag="PLW95_05555"
     CDS             17172..18467
                     /locus_tag="PLW95_05555"
                     /inference="COORDINATES: protein motif:HMM:NF014135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOV22129.1"
                     /translation="MKYLILILFLITYFGIVLKRDKSKISNIFVYGCVFLFIILKAIN
                     FKDIFSFINYNVLGIFLGTSILSFLFTNSKVPAHIVSKIEQKNFSVGLIYLFICITTS
                     FISSFIENVATILIMAPVALEFTKKNYLNPVPLFIGMMVSSNLQGCATMIGDSPSIIL
                     AIESNMNFLDFFYMPAKKLGLTSGKLGLFFFVQFGAILSFFVLYFFFRKEKGQIKFKE
                     EEYKVKTYLPTFSIILMIITLAITSFFQDKFSFFPACICLFFGIITLLIYLKKFKDEK
                     ISIKHIDWDSFFLLIGIFILVGTLKKFGFIEMIADFLIKTGGKNPFILFNLIVWISVF
                     ISAFIDNIPYTMAMISGIQILCTKLSLNPYLFLFGLLLGTCLGGNITPIGASCNVVGM
                     GILKKNGYKVKFSDFVKIGLPFTIIAVAGSTLLLWVVYT"
BASE COUNT         6444 a         2514 c         3181 g         6456 t
ORIGIN      
        1 aatatcggcc cttttaaata gacatttttt tatcaccctt gtcatataca ataaagaata
       61 cttatatttg gtctgatact tagacaaaag caaataataa atcattgcag tccatatttg
      121 ggttaaaacc gcatttttag ttgtccctaa aaatgttttt attttaagat tttgttttat
      181 ccatttgaaa aatatctcta tttcccacct tgttttataa atctgactta ttgtatagga
      241 tgataagtgg aagatctaca tttactaacc caatatgata ccacgcattc tgatgcatct
      301 ttaatcccgt ctctatatcc cgcaaacttt tcttccctgt tatgtgtgca tataaattta
      361 ccaataattg attccatgtt gtaaaatgtt tggtatatct attcacttgt tctttcttta
      421 cttccttatc aaattcatat cttggcaaca tatttagcat ctgattaaaa ttgtgttaat
      481 atattttata ccgaacatcc tttttagcag gtaatgttta tttcttattt taccttattt
      541 taggaggtca gttctaaaaa acttacttaa atttttattg gacagtaatg aaaaaccata
      601 gccaattttt gcagaatctc atgtaacgaa aggagagaaa aatggtaaaa gtatgtatta
      661 ttggagcagg tggtgaggca aatttagttc attatccttc tcttacaaag atggagaatg
      721 ttgaaattgt gggtatatgc gatttgaatc aggaacgatt gagacagaca tccgaaaaat
      781 ataaaatttc caatatttat actgattata aaaaaatgtt agaagaaaaa aatcccgatg
      841 ctgtttatat aattgttcct cctcattatc tatatgatat tgttatgccc tgcttagata
      901 gaggatttaa tatttttaca gaaaaaccat tgggaatgac agcagaacag gcaagacaaa
      961 tggcaaaatt agcagaaaga aaaggtgttt taacaatggt tggttttaac agaagatttg
     1021 cgcctttaat tgttgaagga aagaaaagaa taaaagaaag aggagaagtt gtattagctg
     1081 aggcaagatt ttttcagaat tatttagata gacctcttga ttataacggt gcgattgatg
     1141 ttttaacata ttttgttatt cattcagttg accttttaag atatattgga ggtgaagtaa
     1201 aacaggttaa aagtatggtt aagggggtat ttgcggacta taacaacatt ttttgtgctt
     1261 tatttgaatt ttcttcagga gcaattggga ctttgtcagc agactgggca agtggaaaaa
     1321 gagtttgttc catagcaata catggtaaag gaattgctgc ttttattgaa catgagaaac
     1381 aagcaataat ttattctgat ggaaaagaag atggagaatg tatagatgta aaagagttca
     1441 caggaaaaaa agaatttttt gaaattgcgg gttttttaga tgagcataaa tattttatag
     1501 aatgtatgga gaagaaagaa ttaccagaaa caaatttttc tgatgctgtt aaaacaatgg
     1561 aacttattga gagaatatac agaaacacta tttagttctt actttgtttt gaatttatat
     1621 ttttcaagaa aaaataaaaa aagaagaaaa aaatggatag aataaaaatt ggggtgattg
     1681 gtgttggagt aatgggatat gcacactgtt taagtgtgaa agaaattgaa gaaagtgaat
     1741 taacatgtgt atctgatgct gcggaaggac ttgcaaggaa aagaggagaa gaatttggag
     1801 ttaagtattt tactgattac aaggaattga taacaagtgg tctttgtgat gcagttattg
     1861 ttgcaacacc ccattggttt catccagaag tatctatttt tgcttttgaa aatggattgc
     1921 atgttttaag tgaaaaacca attgcagtca ctgtttctgc tgctgataaa atgattgagt
     1981 cggcagaaaa aaataataga gtttttgcag ttatgtttca aaaaagaacg aaatattttg
     2041 taagaaaagc aaaggaaata gtggatagcg gaaaaattgg agaaattata aggacagtct
     2101 gtattgaccc gtggtataga actcaggaat attatgataa agatatctgg cgtggtacct
     2161 gggttggaga aggtggcggt gtcttactaa cacaggcacc tcacatgatt gatattttta
     2221 ctcatttagc gggactaccc tgtaaaattg aagcaaaaac aagaacaaaa ctgcataata
     2281 tagaagtgga agatgaagta tctgcatttt tagaatatca aaatggtgca tggggatatt
     2341 attacacttc tacatgtgaa ccaattggtt attattataa ctgggaacca gttggccctt
     2401 tttatttaga ggtctctggt gataaaggga aaattgttat aagaggggac cagattgctt
     2461 tttacaatta tgaggcccca ctctatgaaa cttcaatgga agaatttact aaaaaaattc
     2521 caaatatatg ggaattatca acaacacccc tgaaattaga agaaggacaa attgttccta
     2581 cttcaaactt tcaaacaggg cataaagaaa taataaggaa ctttgtcagg agtatccttt
     2641 ataatgaaaa acttattaca ccaggaaaag aaggactgaa aacaattgaa tttatgaatg
     2701 catgtctttt atcaggaaag aaaaataaag ttgtaaatat ccctgttgat agaaaagaat
     2761 atgatgattt aatggaagaa ctgaaaaaaa cttcaaaggc aaaaaattta tagtaataaa
     2821 tagcaattta aaaatgttta aagaaataat aaatgaagtt atgtattatg gaaaagaata
     2881 gcaggttagg atatagggag gaagggataa tgaaaaaaat tagaatcgga cagattggta
     2941 tgagccatga gcatgcttca gcaaaaatga tgacattgcg gcaactcaca gattattatg
     3001 aggtagttgg tatagtggat gacctgaatt ataatgcacc taaatatcct ggtatggata
     3061 tggaatgtta tgcaggtata agacgtatga gtgaggaaga acttttcaat gttaagggac
     3121 ttcaggctgt tgctgtagaa actgcaaata ctgaattggt tcctactgcc atacgttgca
     3181 tgaaaagagg attgcatatg catctggata agcctggtgg agaaattatg gaaccgttta
     3241 cagaattgat agagggttgt aagagaaaaa atcttgctat tcaacttggt tatatgtaca
     3301 gagttaatcc agcaataaaa ttctgtttta aagcagtaag ggaaggatgg ttgggagaaa
     3361 tttttgaaat ccatgcgatt atgaatcgtt ttgatgacga gaattaccgt aagtatttat
     3421 ctaatttcaa aggtggtgct atgtttaatt ttggatgtca tcttattgat ttagttatta
     3481 gtataatggg acgtcctgaa aatgttgtgt cttttcagaa aagtactgct gatgatggac
     3541 taaatgataa tgggctggct gttctggaat attcaagagc aactgcaact gtcaattctg
     3601 ctattactga ggtagatggt tttaaatatc gccgttttat tgtatgtgga actaaaggga
     3661 cggttgaaat ctgtcctctg gaacattctg ctgacctgta ccgtattgaa ccattatatg
     3721 caagattaac actccttgaa gataactcag aatattcggc aggaactcat aaggttaatg
     3781 taggagtaat gaatggtaga tatgaaaacc aattaattga attagcacat attatcaatg
     3841 gagaaatttc caatccctat acatacgagc atgaacttct ggtacaataa gttttacttg
     3901 ctgctgctgg atacactgaa tggcaataaa aaaattgatt tataggagaa aatataagat
     3961 gaaaaagatc caaaaaagta aaaggacaat tttgaatgtt ccttctgtaa aaaaatggcg
     4021 tataggagtg gtaggatatt atagttgttc aggattaggt ggtcatggtc tacatttagc
     4081 gttttctgga ttacctggag ttgaaattgt atcagtagca gaccccgatg agacaggacg
     4141 taatatgctt cagaaagaaa caggagcaaa aaatagttat agtgattggt cttatcttat
     4201 tcaacatgat aaaccagatg tagtttgtgt atgttctcgt ctaccttcac agcatacgga
     4261 tgttgttgtt gcagcagcag aggcaggttg tcatatatat tgtgaaaagc catttgctca
     4321 aacattagaa gatgcagata aaatgataaa tgcggctgct tctaataatg ttctgatagt
     4381 agttgcacat ttgggtaggt atgctccaat gtatcaggtt gccagggaaa tgattcaaaa
     4441 aggagatatt gggcgaccac tttcagtata ttgcagagga aaggaagatg aacggggagg
     4501 tggtgaagat atgatggttt taggaacaca tttatttgat atgtcttgtt ttttcttcgg
     4561 ataccctgag tggatttttg gtaatataac agtaaaaggt cggaaaatga cactaaatga
     4621 tgtgactgaa cctaaagagc caataggacc agttgcaggt gatgaaatag tatctctttt
     4681 tggattttcc aatagtgtgc gtggttattt tgaaagtaga aggggccttt ttgataagaa
     4741 aataaggcgt atgggtatat ctgttgctgg ttcagaagca atacttgcta tgcgatttga
     4801 tgcaaaaccc agattatata taagtactga cagacgtcct tttgaaaaac caggagattt
     4861 tgatgaaatt gatattcctt ctgtgcttga aattccaggg acccagccgt taagtttatc
     4921 agttagcgac ccagaaaacg atatgccttt tacttactgt aatcgttatg ctgcggttga
     4981 tttactttgt gcaatagtgg aaggacggga acctatatca agtggaaaag atgcaagatg
     5041 ggcattagaa atgatttttg gggtttatac atcccattta gcaggtaaat ctgttgattt
     5101 ccctttaccg aacagaaagc atcctttaac aagaaaatag tttaaaaata tatcaacaaa
     5161 tgataaaaaa ggagaaaatt ttatgaaact aaaagatgaa gtagcaatag ttactggtgc
     5221 agcaggatat attggtagta caactgctaa aaaattagca tctgaaggtg ctaatatagt
     5281 agtatgtgac atgaatattg atggttcaca acgggtagtt gaagatatta tcagtaaagg
     5341 tggtaaagca acagcaatac aattagatgt aaccaataca gatagtattg aaaatatggt
     5401 taaatgtgta attgagaaat atggtcatat agatatatta attaatgtgg cgggaggtag
     5461 tgagagagga agaaatgcac cagtgcataa tttaaaagag gaagtaatta gagaaaccat
     5521 tgctgtaaat ctattaggag ttattttttg ttctcgtgct gtagttggtt atatgataga
     5581 aagaaaaaag ggaaaaattg taagcgtagc atctgttctt ggtttaagag gaaagaggaa
     5641 cttttcatca tattctgctg ccaaggcagg tgttattgtt ttctcaaaaa ctctggctat
     5701 ggaagtaggt ccttacaata tcaatgttaa ctgtgtttcc ccgggaaata tacctcgtcc
     5761 gggagaacca aatgctcata ttcctacaaa aaattatttt ggtcgtattc caacagcgga
     5821 ttgtgttgcg aaccttattt attttttagt atctgacgag gccgatttta ttactgggca
     5881 gaactatatc gtggatgggg gagaaacttt ggggttgaaa ggtgcttaaa ttttacctga
     5941 ggaacatatt ttttcgaaat aatttctaat tctaaataat aaatattttt gagagtacac
     6001 agagtgggga tcaaaatgcg tgcaatctta gttgataaga ataaagagct tgtttgcaaa
     6061 gatattccgg aaccagaagt taaagataat gaagttttaa ttgaagttca tgctattggt
     6121 ataaacagag ctgacttaat gcaaagggat ggagaatatc cttctccagc aggatggcca
     6181 gatataatgg gtcttgaggt agcaggtatt gttcttagtg ctccatcaca aagtagatgg
     6241 aagaagggtg ataaagtttg tgcattactt ggtggtgggg gttatgcaga aaaagtatct
     6301 gttcctgtaa atatggtttt gcctgttcca gagggattat ctatggcaca agcatccgca
     6361 ataccagaaa tttttgcaac ttcatggctc aatctttgta tagaaggtaa aatgaagcat
     6421 ggtgaaactg tccttattca atcaggagca agtggagttg gtactgctgc aattcaaatt
     6481 gttaaagcat ttgggggaaa agttataact actgtgggtt cggataaaaa agttgagttt
     6541 gttcgtagta taggagcaga cataattgtt aacagagaaa aagaagacct tttatctgta
     6601 cttgaaaaat atcctgtgga tatatcgctt gattgtatag caggtccttc tttgggtaaa
     6661 tgtatagaga aaatggcaca gggtggtcgt tggatagtta ttgctactct gggtggaata
     6721 atgacagaaa tcaatatgaa catatttttt aaaaaaggaa ttagaattat tggcagtact
     6781 cttagaaata aatctactga aatgaaagga aaaatacttg ctgatatgga atctgcactc
     6841 tggcctaagt tttcatctgg tgatattaaa cctgtaattt ataagatact gccaatagac
     6901 caggtatctg aagcccatgc aatattagag cgtcgggaaa atcttggtaa agtagtcctt
     6961 actatttctg aataactttt agtatgtgta ttttatttat gtactgttgt aaaattatat
     7021 aggatattga aacgaataga gggaatatcc tactgcccgg tataaaatgt aaaagcccaa
     7081 aaatattaag aggcaacagg agattttaaa gaaaatgcga gttggttgtt tctacaatca
     7141 attacaacat tatctccttc tttgaactca ccacttaaaa ttttacctgc aagaacattt
     7201 tcaatttctc tctgaataag tcgttttaat ggtcttgctc caaatgtttc atcatatccc
     7261 ttttctgcaa tatattcttt tgctttatca gttgcagtaa gttctatttt cttttcttca
     7321 agtcgttttt gtaaataaga aagttgaata tcaacaattt ttataatatc ttctttcttt
     7381 aatttattga atataataat ttcgtcaatt cggtttaaaa attccggtct aaaattactt
     7441 ttaacaactt ccattatttt ttctttcatt gcatcttcat ttttaaacat tgtaatatat
     7501 tgacttccta aatttgatgt cattataata accgtatttc taaaatcaac agttctaccc
     7561 tgcccatcag taagtcgtcc gtcatcaaga agttgaagta aaacattaaa aacatcttga
     7621 tgtgcctttt caatttcatc taataaaata acactataag gtcttcttct aattttttct
     7681 gttagttgtc ctccttcttc ataaccaaca tatccaggag gtgctccaat aagacgggaa
     7741 acagaatgtt tttccatata ttcactcata tctatcctaa ccattgcatt ttcgtcatca
     7801 aacaaaaatt ctgcaagtgc ctttgcaagt tctgtttttc caaccccagt tggaccaaga
     7861 aaaataaatg agccaattgg tctattggta tctgaaagtc ctgctctact tcttcttata
     7921 gcatttgaca ctgcttcaat tgcatggtcc tgccctacga ctctcttttt aagtcggctc
     7981 tccatctgaa gcaatttttc tttttcacct tcaagcattt tagaaacagg aatacctgtc
     8041 cattttgaaa caatttctgc tatatcttct tctgttactt cgtctttaac aagtccacta
     8101 ccttttttct gtatctcttt taatttttct gtttcttttt caagttgttt ttgcaattca
     8161 atcaacttcc catatttgta ttctgctgct ttatcaaggt ctccttctct ttctgctttt
     8221 tcaacaatac ctcttgcaac ttccatttgc tgttttatat ttcttatttt ttcaacaact
     8281 tctttttctg ctaaccattg tttgttatat ttttctcttt ctttttttaa tttttcaagt
     8341 tctttctcta ttttctccag tcgctctttt atttcttccc ctgtttcctt ttttaatgcc
     8401 tgcttttcaa tttcaagttg tatcattttt ctcttaatct catcaagaac ttctggcata
     8461 ctatctattt ccattcttaa tttactcgct gcttcatcaa taaggtcaat tgctttatca
     8521 ggaaggaatc tatcagtaat atatctatga gaaagaactg ctgcggaaac aattgcacta
     8581 tcagttattt ttacaccatg atgtacttca tatctttctt taagtcctct taaaattgat
     8641 attgtctctt caacacttgg ttctttaaca aatataggtt gaaatcttct ttctaatgct
     8701 ctatcttttt ctatatattt tctatattca tcaagagttg ttgctccaat acatcttaaa
     8761 agtcctcttg caagtgctgg ttttaacata ttggaagcat ctatcgcccc ttccgctgca
     8821 cctgctccaa caagagtatg taattcatca ataaaaagaa taatttcgtc ttctcttttt
     8881 tcaatatctt ttaagagtgc tttcagtcgg tcttcaaatt cacctctata ttttgttcct
     8941 gctacaagta aaccaaggtc aagagataga atccttttat tttttaaatt ttcaggaaca
     9001 tctccactta caattcttct ggcaagtcct tctactattg ccgtttttcc aacacctgct
     9061 tctcctatta aaacaggatt attttttgtc cttcttaata gaacctgtat aactcttctt
     9121 atttcttcat ctctaccaat aacagggtct aactttcctt ttcttgcaat ttctgttagg
     9181 tcctttgtat acttttctaa aacattatat ttgctttctg catctttatc ctttattttt
     9241 tctccccctc ttaaattgtc aataattttt ataattcgct gactaaaaat atcatatttc
     9301 ttcaaaattg ttttagcagg agaatctact tctgataaag atattaaaag atgttcaact
     9361 cctatatatt catctcctaa tgtctttgcc tgtttacctg caatataaaa aacctgattt
     9421 aaagaaggag taatataaac agatgtttgt gaagaaaaaa cttctggaat tttcttcagt
     9481 tctctttcta tatcttcttt caaattttca actttgacat tcatctgttt tagtatctcc
     9541 gcagttgttt catctccatc aacaagagaa taaagcaaat gcaaagtatc tattgcctga
     9601 tgacctctct cctgggcaac tgcttgcgaa ttcattattg cttcttgtgc ttttattgta
     9661 aatttttcta aatccatttt atcacctctt ttaatttatg ctatttagat ggaattatga
     9721 taaaaaagtt tcatttttta taatgaaata gtcataagag gtagcaatat tttgaaaaaa
     9781 ttagaactct ttactaaaat ataaatggga tttattaaac acttatttta tgttataata
     9841 ctcttcaatt tattataatc tattatttct tttttaaaaa atgaataatg aatttaaaac
     9901 aaaaatagta ggggaagtta tttgtggtta tagagttatt gataaaatat ggcagggagc
     9961 aacttctact gtatataagg gtataaaact caataattct tataatagcg taatagcaat
    10021 aaaaatactc cattcttata gaaattcaaa acaatattta aaacagttta ttaaagaata
    10081 taaaatccag aaatcactta accatccaaa tattgttaag gtctttggtt ttggaaaaca
    10141 agaaaatctt tattgtatat ttatggagta tattgaagga aagacattac gattaacata
    10201 tgatgaaaat ctaattcaag agaaaaattt gttccatatt ataattcaga ttggtaaagg
    10261 tatatcatat attcatagta agaatataat tcataatgat ataaaaccag aaaatataat
    10321 tgttgaaaag tcatgtagat gggctaaatt gaccgatttc gggtatgctg taaaaaaatc
    10381 tttttttaga agaaatattt caattcaagg tggcacggaa aattatattg cccctgaaag
    10441 aaaaaaagga atttcaaatt tcaaaacaga tatttattct tatggtatga taattgaaga
    10501 attgcttttt gaaaaattac catcagaaga attatacgac attgtggcaa aaacaacaga
    10561 caaagttccg tctcaaagag acgaaaatgt tgatgatata gttaaccaat tagaaaaaat
    10621 ttataaaaga atatacaaat gaaattttct ataatatctg atattcattc taattatgaa
    10681 ggactgactg aaattttaaa atatattttg actgaaaaaa ccgattatct ttttgtatgt
    10741 ggagatataa ttggctatgg tcctgaccct gaattatgtc ttgaaaaaat tttttctatt
    10801 actgacctta ttgtaaaagg aaaccatgaa gaaggaataa taaccgggaa ttattcaaga
    10861 tttaaaaaac ttgcaaaaat ttctatagaa tggacagaaa agaaaataac aaattatata
    10921 gaaaaaataa aatgccttcc agtaaaaata acttttggag atataacttt tgttcatgcg
    10981 tgtttatccg acccgattta caaatatatt ctttcaaaaa aagatgctga aagggaattt
    11041 gaattattta gcaataaaat ctgtttttat ggacatactc atattccttc tgcttacaaa
    11101 aaaaatttaa aaacaaataa tgtagaaaca attatacctg attttaatgg aaagatgtgt
    11161 tttaaaattg aagatgacta taaatatttt ataaatgttg ggagttccgg acagccaaga
    11221 gatggattac caatggtgtg tatatcaatc tatgatagtg aaagcaaaat atttaaactt
    11281 tctaggctta attatcccgc agaaattact aaaaacaaaa ttcttgaaag ggggcttccc
    11341 tcttctcttg gtgaaaaatt tttaaaaggt atttaattta ctttttagga tttaattggt
    11401 ttatttttgt tataatatcc tttacttttt cacaatttcc attaagagat aaaatatcct
    11461 cccatccctt attttctttt gttttcatca acttatcaat ttgtattcca tttacctgac
    11521 ttactgcaca cccaatagca aggtcaaaaa aagaatattt ttctcttaat ttatcaaaat
    11581 cattagaaga aatttttgta attttacaaa ttttattctt tataatttcc gccatatagt
    11641 cagttgttgc ccttttttct atttcaagag cagatgaata aattccaaca agaacttcat
    11701 tgcctgtttt gattactttt tcaacttcat ctattgatgt tttgacttca tcaattgttg
    11761 ccttcttagt tgcagaatat ccactaattg aaaaaagaaa taaacaaagt acaacaagca
    11821 cttttttcat ttttcacctc catttttgtt tatattttac caatgtataa aaattttgtc
    11881 aaatatgtta ttataaaatt atggaaagaa aaattatgga tgaaaaacag atgagagaaa
    11941 aacttatatt tctttctaaa aaaataaaaa atgataagca actttctgat tttgtaatta
    12001 ttgggataaa aaggagggga gcagttttag cagatagaat taaaaaatta ataggaaaag
    12061 aaaatcttga tgtcgggtat cttgatataa gtttatatag agatgattta agcaaaattg
    12121 gtccaaatcc tattatttct caaacagata ttttattttc aatagatgat agaaaggttt
    12181 tacttgttga tgatgttttg tatactggaa gaactgtaag ggccgcccta gatgcaattt
    12241 ttgattttgg ccgtccatca cttattaaac ttcttgtttt tattgatagg ggacataaag
    12301 aacttccaat ttgtgctgat tatgttggtg aagaagtaaa aacaaaaaaa agtgatatag
    12361 ttgaagtaaa agtaaaagaa atagatggag tggatgaagt agttattgtg gagagaaaat
    12421 gacgtggaaa agaaaggacc tgataggact ggaagattta agtaggtatg aaatagaaac
    12481 aattttagaa acggcaaatt catttaaaga agtcagtagt cgttctataa aaaaagttcc
    12541 tgctttaaga ggtaaaacag ttgttaattt attctttgaa ccaagcacga gaacaagaac
    12601 ttcttttgaa cttgcagcaa aacgactttc agcagatgtt ttaagttttg atgtttcaac
    12661 ttcttctata tcaaaaggag aaacaattgt tgatactgca aaaaatattg aagcaatgaa
    12721 aattgattgt tttatagtaa gacattcagt tgcaggagca tcagaactta taagcaggaa
    12781 tgtaagaagt tctgttataa atgctggtga tggttgtcat gaacatccaa cacagggtct
    12841 attagacata tttactgtaa aagatgtttt tggtaaaata gaaggaatta aaatagcaat
    12901 tattggagat attttacata gcagagttgc aagatccaat atctggggat ttacaaaact
    12961 taaaagtaaa gtaattgttt gtggacctcc acctttaata ccatctggca tagaaagaac
    13021 aggagtaaaa gtaacttata attttaaaga agcaattgaa aattcggata tagtttatct
    13081 tctaagatta caaaaggaaa ggcagaaaga aaattttctc ccttctttaa aggaatatag
    13141 tttaacatat ggattaaatg aagaaagaat aaaagggata aacaaaaata tatttattat
    13201 gcatccagga cctataaata gaggaattga aattaaatct gaaacagccg atagagaaaa
    13261 ttctttaatt ttacagcaag ttacaaacgg aatagcagtt agaatggctg tcttatattt
    13321 aacaattgga agtcaaaaat gaaaatacct acttatccag caattgatta taaaaattca
    13381 ttaaatgaaa gacaatatga aataattaaa aaagcagatg gccattctat tgtaattgca
    13441 ggtgctggaa gcggaaaaac aagagtttta gtttataggg tatgctatct tcttgataaa
    13501 ggaatacctc ctgcctcaat aatgcttgtc acttttacaa ataaagcagc aaatgagatg
    13561 atagaaagag ttaaagaaat agtaggtgtt tatccaaaag ggttatgggc aggaactttt
    13621 catcatattg ggaatattct tttaagaatt tatgggaaaa atataaatat tccttctaac
    13681 tttacaatta tggatgaaga agattcaatt tctttaataa aagaaataat agataaatta
    13741 ccaaataaag atgattatcc atatcccgga aaaataaaga attttttaag tttatcagta
    13801 aatacaggcc aaccaataag agatataata gacactcgtt ttattgaata ttcaaacctt
    13861 attccacaga ttgaaagaat ttcaaaggac tataccaaga aaaaaaaaga tttcaactta
    13921 cttgattatg atgatattct tcttttctgg tatagattga tgaaagacga aaaagttgga
    13981 atacaaatat ctgaaaaatt tttatatata attgttgatg aatatcatga cacaaacaaa
    14041 ttacaggcac agatacttta tcaggtagca aaagttcata ggaatataat ggtagttgga
    14101 gatgatgctc aaagtatata ttcatttaga ggggcaacag tggataatat cctggaattt
    14161 cccaaaattt atcctgatac caaaatattt tatttagatg taaattatag aagtactcct
    14221 gaaattcttg attttgcaaa taatattatt tctcataata aaaaccagtt tcctaaaaat
    14281 ttaaaaagta taagaaaaag gggtgttaaa ccacttatta ttaaatgtta tgatataaaa
    14341 gaagaaagta tatttgtttc aagaaggatt tttcaacttt tacaatcagg agtaattcca
    14401 tctgatattg cagttctttt cagatcaaga tatcaatcag cagaattgga aattgaactt
    14461 aataaattaa aaattcctta tgttataaga ggcggtttaa gattttttga acaagcacat
    14521 ataaaagata ttatatgttt ttataaagtt ctgataaatt ttaaagatga aatttcctgg
    14581 aatagaattt tctttctaac agaaggcatt gggaaaaaag gagcagaaga attaataaaa
    14641 gttataagag aaagtgatga aatagaagtc cttgaaaaaa aaattgaaga tataaaaata
    14701 aattcaaaag ggcttaaaaa ttttaaaaat caattaggtc ttattaagaa aataaaaaat
    14761 ttaaaaatat cagaaggtat tgacttaatt cttatctctt tttattcaga atacttgaag
    14821 aaaaaataca aagacgatgt tgagagaaaa caggacatag aagcactaaa agaaattgca
    14881 gatatgtact cttcattaac tgattttctc tcagaagcaa cattacatga acatacaaaa
    14941 ggagaaataa ggcagaaagg aaattacata gtactttcaa caatccatca ggcaaaaggg
    15001 cttgaatgga aatttgtatt tataataggt gtttgtgata atcatttccc atatatttcc
    15061 gctaaaaacg acattactgc attagaagaa gaaagaagaa ttttttatgt aggagtaaca
    15121 agagcaaaag aagaccttta cattacttat tatataagag atttttatag gaattattat
    15181 tacaacaaat caatttttat agaagaaatt cccccgtatc tttatgaaag ttgggatttc
    15241 cagtaaataa aaggagatga aatggagaaa aatatattta caaaaaggtc aatattgata
    15301 ggcacaattc tatcagtttt tattagtgtt agcgatgttt ataatcttat ggtgattcag
    15361 ggttctgata tgacaattga ttttacaact ggtgcagcaa tatatctaat attttggata
    15421 actctcttta attacctttt aaaaaaaatc tctccgaaat tctctctttc tattgcagaa
    15481 atgattttaa tttatattat gatgattgtt tcctgttcaa ttcctacaat gggacttaca
    15541 ttatacctta ttccacttat agcaggtaca aaatattatg caaatgacca aaacaactgg
    15601 gcagaccttt ttataaaaca tttaaagaac tggactattc taaatgataa caatgcaata
    15661 aactggtttt ttgaaggaat tccaaaaggg caaagtatac catgggcacc ttggataaaa
    15721 gttttaattc tatggatacc tctaataatt gctctctatc ttgcaatgat ttttataatg
    15781 gtaattttaa gaaaacaatg ggttgaaaat gaaaaattga attttccttt aacgaaagcg
    15841 cctatttcat taataaaagc agatgaaaac aaaattttta ataataatct tttctggctt
    15901 ggatttgcta ttccatttat tattggatgt ataaatggat tacatttcta taatgaactt
    15961 tttccaagaa ttcaacttgc tacttctact ccaattttta gaagaactat gagtttacca
    16021 tatagaataa gttttcctat gataggattt acttattttg ttcctttacc tctttctttt
    16081 agtttatggt ttttttgtct tctttctaca atacaacagg gtttttttaa tataactggt
    16141 tttggaattt cagaattttt accatataat gcggaaaggc caattcttgg atggcaatca
    16201 ttgggttctt tattcatcat agttatttat ggtctatgga ttgcaagaaa acaaataaaa
    16261 gatgttttca gaaaaggaat tggaaaagaa aaagatgatt cttctgaata tgaaattgtt
    16321 tcttataaat ttgctttttg gggattggta atttctattt tggtaatttt ctggtggatt
    16381 gttttaagtg gacttcctcc ttttccatca atatttttta ttatttttat gttcataatt
    16441 tttattggta taacaagggc tatttgtgaa ggcgggatct ctacaacaag agcacctgtt
    16501 attgcccctg ttataacaac atcatttttt ggctcaaaaa atttgggacc ttctgcttta
    16561 ggtcctcttg gtttaacttt tgtttattca tctgatgtaa gaacttttgt tatggcttct
    16621 gttgcaaatg gattaaaaat acttgaagaa gttaagggga agaaaaaaat aatttttgga
    16681 gcaattttaa tatcaattgt aactactttg atttcttcaa tatggacggt tatctatttg
    16741 ggatataaat atggaggaat aaatgcaaac gcatggtttt ttgtaaatgg tcctcaatat
    16801 ggttggcaat atattgttca ggaattaaaa accccatctg gtcctgatat gatatattgg
    16861 ggattttttg ctataggtgc tgtttttaca attttcttac aatttatgag gatgagattt
    16921 ttatggttcc catttcatcc tcttggtttt gctttttcaa caataatgct tacaaacgag
    16981 ttatggtttt caatttttct atcatggctg tttaaattta tgattttaag gtatggaggg
    17041 gcaaatgtat atgaaaaagg aaaaacattt tttatagggc taatagttgg acaatttatt
    17101 gtaagtggaa catggctttt tattgatttt ttcacaggac gcacaggtaa tattctattc
    17161 tggatataac gatgaaatac ctaatactta tccttttttt aattacatat tttggaattg
    17221 ttttaaaaag agataaaagc aagataagta atatatttgt ctatgggtgt gtttttttat
    17281 ttattatttt aaaagcgata aattttaaag atattttttc ttttattaat tacaatgttt
    17341 taggtatttt tcttggaaca agtattttat cttttctttt cacaaattca aaagttcctg
    17401 ctcatattgt aagtaaaatt gaacagaaaa atttctctgt tggtttgatt tatcttttta
    17461 tatgtattac cacaagtttt atttcatctt ttattgaaaa tgttgcaaca attttaatta
    17521 tggcacctgt tgcattggaa ttcacaaaaa aaaattactt aaatcctgtc ccccttttta
    17581 taggaatgat ggtaagcagt aatcttcaag gatgtgcaac aatgattgga gatagtccaa
    17641 gtataatact tgcaatagaa tcaaatatga attttcttga ttttttctat atgcctgcca
    17701 aaaaattagg acttacatct ggtaaattag gacttttctt ttttgttcaa tttggagcaa
    17761 ttttaagttt ctttgttctt tacttttttt tcagaaaaga aaaagggcaa ataaaattta
    17821 aagaagaaga atataaagta aaaacatact taccaacatt ttcaataatt ttgatgataa
    17881 ttacacttgc aattacttct ttttttcagg acaaattttc ttttttccct gcctgtatct
    17941 gtcttttttt tggaattata acacttctta tctaccttaa aaaattcaaa gatgagaaaa
    18001 tttcaataaa acatattgac tgggatagtt tctttttgct tattggaatt tttatacttg
    18061 ttgggacatt aaaaaagttt ggtttcattg aaatgatagc cgacttcctc ataaaaacag
    18121 gtggtaaaaa tccatttatt ctttttaact taattgtctg gatttctgta tttatttctg
    18181 cttttattga taatatccct tacacgatgg caatgatttc aggaatacaa atactatgca
    18241 caaaactttc tttaaaccca tacttatttc tttttgggct tttattgggg acatgccttg
    18301 gtggtaatat aaccccaatt ggagcaagtt gtaatgttgt tggtatggga attctgaaaa
    18361 aaaatgggta taaagtaaaa ttttctgatt ttgtaaaaat aggacttcct tttacaatta
    18421 tagcagttgc tggaagtact cttcttctct gggtagtgta tacataaata ctcactcaaa
    18481 acagtgcctg agaagtaaaa agtaaagaga tataaaaatt cataggtaat tttggcagac
    18541 ctaaaaaatg gaggaaaaaa atgattaata taagaagggc aattttaagt gtttc
//
LOCUS       DAOOVW010000032        18026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_2134_length_18026_cov_8.371320, whole genome shotgun sequence.
ACCESSION   DAOOVW010000032 DAOOVW010000000
VERSION     DAOOVW010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 18026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18026
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2134_length_18026_cov_8.371320"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..612
                     /gene="gcvH"
                     /locus_tag="PLW95_05560"
     CDS             235..612
                     /gene="gcvH"
                     /locus_tag="PLW95_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOV22130.1"
                     /translation="MENKGLLYTKEHEWVKIEGKVGLVGITDYAQNSLGDIVFVDLPP
                     VGRKVKQFETCGTIESIKVASDIFSPISGKIIEVNENLKETPEIINKAPYESWLFKIE
                     VEDEKEKENLMNEVEYQQYIKNL"
     gene            615..1958
                     /gene="gcvPA"
                     /locus_tag="PLW95_05565"
     CDS             615..1958
                     /gene="gcvPA"
                     /locus_tag="PLW95_05565"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOV22131.1"
                     /translation="MNYTPHTDLEIKEMLKEIGIESIDELFKDIKHILLPKSFNLPEG
                     KSEMDVLKYIEKLSYKNYTDLTCFIGAGFYDHYIPSVVDSISSLPAFYTPYTPYQPEA
                     SQGWLQALYEYQSVISNLTDMDTANASLYDGGTALYEACMMSTKITGRKKIIMDAGVN
                     LIYRTMLYSYTFNLDINFVEIPNIDGITSREKIIKSLNEGNVSAIILQNPNFFGYIDD
                     YSDIVEMAHKVGTLVILSVYPVSLGLLKTPGEMNIDIAVGEGQSLGLPLYFGGPYLGF
                     ISTKKDYVRKMPGRIIGRTKDKNNKDCYVITLQTREQHVRREKATSNICTNEALCALR
                     SLIYLCLLGKEGIKELAQLILEKASFTKELIKKVPGVSICKKSLPTFNEFTIKLPKNA
                     DEVVNKMIEKGYIAGFPLGRFYKGMDNYLLIAITEKRTKEEIVKFVESLEAVLCD"
     gene            1949..3403
                     /gene="gcvPB"
                     /locus_tag="PLW95_05570"
     CDS             1949..3403
                     /gene="gcvPB"
                     /locus_tag="PLW95_05570"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015752006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOV22132.1"
                     /translation="MRLIFEQGRKGRRGFKIPKKDVPISKVDIPEKYKRKTEINLPEV
                     SELDVVRHFTNLSKLNFCVDTNFYPLGSCTMKYNPKFIEKAANLNGFRDLHPLLPQLS
                     GGGRLTQGALEILYRIEQLLCEITGMDAFTMQPLAGAHGELTGTMLISAYHKDKGNKR
                     KYIIIPDSSHGTNPASAAIAGYEVIVVHTDKDGFMDFDEFEEKMNENVAGVMLTCPNT
                     LGLFNPKIKEICDISHKYDALVYYDGANLNAVLGKMRPGDIGFDIVHLNLHKTFATPH
                     GGGGPGAGPVGVKKHLIEFLPISRVIMKKDGTFMLDYDYPKTIGYIAPFYGNFGVIIK
                     AYAYILLLGKEGLLDVSDKAVLNANYLRAKLSKYYEIPYDKTCMHEFVLSVEDKKEKY
                     GVSANDIAKYLIDKGIHPPTVYFPLIVKEALMIEPTETESKETLDEFIDVMVEIAKLC
                     EEDPDKIKNAPYNTYISEPDEVKAAKDLDLSYKK"
     gene            3400..4191
                     /locus_tag="PLW95_05575"
     CDS             3400..4191
                     /locus_tag="PLW95_05575"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HOV22133.1"
                     /translation="MKSRYIFSSFSSPYENMAIDESLFISYFDKKVPTFRIYGWRPYS
                     FSIGYSQKAKEILNLEKCKKDNIGIVRRITGGSMIFHADEVTYSIVCSESDIGNPASI
                     KASYKILTSFILRAYEKLGLFPKYAIEFSDIIKEKSSFCFMSQEDYDIIIKGKKIGGN
                     AQKRKKDVILIHGSIPLGDDYKIAQKYTKENLLKLNIRSSCLSEVLERKIDFFEFSEI
                     LKESFKEIFGEFEFDELDKKEINLKEALLKEKYSQDSWNLYYNET"
     gene            4181..5035
                     /gene="lipA"
                     /locus_tag="PLW95_05580"
     CDS             4181..5035
                     /gene="lipA"
                     /locus_tag="PLW95_05580"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOV22134.1"
                     /translation="MKPDYLKKRINLEDTSYVKNILRDLSLHTVCEEAFCPNISECFS
                     KRVATFLILGKICTRNCKFCDIEKGRPEELDKDEPEKVLEAVKKLRLSFVVITSVTRD
                     DLEDGGSEIFKMCVLKIKNFDKNIKIETLIPDFKGNIKALEKVVSVSPEIISHNIETV
                     PSLYSEIRSKANYKLSLSVLKNVKELNNKIYTKSGIMLGLGEKEDEVIKVMDDLRQVD
                     CDFLSIGQYLSPSKNHYPVKEYISPEKFDYYKEKAMNSGFKYVKSGPFVRSSYLAEEY
                     LLSNSKIP"
     gene            5421..5615
                     /locus_tag="PLW95_05585"
                     /pseudo
     CDS             5421..5615
                     /locus_tag="PLW95_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296078.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
     gene            5615..5917
                     /locus_tag="PLW95_05590"
     CDS             5615..5917
                     /locus_tag="PLW95_05590"
                     /inference="COORDINATES: protein motif:HMM:NF014092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HOV22135.1"
                     /translation="MRFFCIADKDSSLGFRLAGVETREVTTKAEVLEAFQVALATDEI
                     GILVITEKAANFIREEIENCIYQKQLPLILEVPSRDTEGISRDISEFLRKAIGISV"
     gene            5926..6564
                     /locus_tag="PLW95_05595"
     CDS             5926..6564
                     /locus_tag="PLW95_05595"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HOV22136.1"
                     /translation="MDIEKYTEDTEQIKEISRRIQEEADYQARDIIQQAKQESENILN
                     IAQQQIASERTKKMADLELELKQMREHIFSSLNLEKRRIELKEKERFIKKILEEVELQ
                     AKSFRTSEEYPNFLMKDILEGISVLDKEELRVIYSSLDEAIFTEAFLQKLSEECHKQL
                     GKKVILHPEKGDFSDIGVKLQSMDGRIVFDNTFSARLARMQNEIYNRLLKEN"
     gene            6627..8381
                     /locus_tag="PLW95_05600"
     CDS             6627..8381
                     /locus_tag="PLW95_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOV22137.1"
                     /translation="MICGKIFRVSGPVVELEEVVNLRMMDMVEVGEDHLVGEVVRLKG
                     DRGYIQVYEDTTSLKAGDFAYTQGFPLYVELGPGLISTIYDGIQRPLEVIKNKYGIYI
                     TRGVHVSPIDKQKKWHFYPSAVEIGKEIASGFVLGEVQETPNLKHLIMLHPNLSGILK
                     WIAPEGDYTVEEKIAVLENNHGKEEIYMYHRWPVRTPRPVKSRFPVSKPLITGQRVID
                     TLFPIAKGGCVAVPGGFGTGKTVVQHQLAKWSDADIVIFVGCGERGNEMIDVLLNFSK
                     LISPKTNRPLLERTIFIANTSNMPVAAREASIYTGITLAEYYRDMGYQVALMADSTSR
                     WAEALRELSGRLEEMPLEEGFPAYLSSRLAEFYERAGNFQTLSGNNGSITIIASISPP
                     GGDFSEPVTSHTKRFVRSFWELDRELANARHYPSISWIESYSEYLDDVKEWWHKNIDP
                     QWETARYAIMDLLQKEQKLLQVVKLVGPDVLPQTQRLILDICSIFKSAFLQQSAFDKV
                     DTYTSPKKQFLMLNAILKLYEKSEELVKKGVTIAEIKATDVYEDILRMKTKYTEEDIE
                     KLEELPVRINTELDELNF"
     gene            8765..10150
                     /locus_tag="PLW95_05605"
     CDS             8765..10150
                     /locus_tag="PLW95_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOV22138.1"
                     /translation="MTEKYSLREYMGLEEIVGPIFIIRDIHNVGYNELVEIVDNQGKS
                     RIGITLEVGKGYAVVEVLGGTVGLSLNESRVRFKGEPLMLGVSEEMLGRIFDGLGNPL
                     DGFPRPAYEEMMNVNGQAINPTARDYPRNIIETGLSAIDVMNTLVRGQKLPIFSGSGL
                     PHDLIAAQITRQARVKEEQFCVIFAAMGIKYDTAQFFIRNFEETGVLERTVIFLSLAD
                     SPSIERLQTPRLALSCAEFLAFKKHMHVLVIMTDMSNYCEALREVSTIRGEIPARKGY
                     PGYLYSDLASLYERAGIIKGSEGSITQLPILTMPNDDITHPIPDLTGYITEGQIVLER
                     ELFSRGIYPPIAGLPSLSRLMKDNIGAGMTREDHPDLSSQLFACYAHVKDIRALAAII
                     GEEELSPLDQLYLKFGDAFEQKFLSQGHYERRTLEQGLNLGWEILSELPADELLRVKE
                     EEIKKYYKGEK"
     gene            10147..10776
                     /locus_tag="PLW95_05610"
     CDS             10147..10776
                     /locus_tag="PLW95_05610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOV22139.1"
                     /translation="MRIQCPATKTNLLKMKKALLLTEEGYQLLEEKRKIIMNALTGLI
                     HIIHQSEEEVATALKEGYNLVDKTICSMGRKKLEEISFSINIQNSISISHRKVMGVSL
                     PDIKLDLQDNPPYYSPYEVSLYTDEVIEHFKKILSLLVELAEKRIALLRLAKEMQKTM
                     RKVNALEKVYIPAYRETVKYIGDRLDEESRDAFSLLKLIKQRHAGNRVT"
     gene            10900..11382
                     /locus_tag="PLW95_05615"
     CDS             10900..11382
                     /locus_tag="PLW95_05615"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOV22140.1"
                     /translation="MAIKNILVSLSVDINSSLVTAKYAIYIAKVLQAKLIAVYVVDKE
                     ALQKLIKARIFIDTEAMEYERDIENEGKIFLDRFKQMAENKEVPFEGIELKGVVHTEV
                     INKIKETNADLLIMGELKEALSLRDTFYNEGALIFFEAPCPVLVVKNKKYVELLYQSI
                     "
     gene            11494..11838
                     /locus_tag="PLW95_05620"
     CDS             11494..11838
                     /locus_tag="PLW95_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22141.1"
                     /translation="MKEVILEVLKEEKCAEEKLESARKEAENIVLEAKKKAQFLIEES
                     SRKTNAEVIKKKAEMEQAYLEEKENILRTVQKETDAYLQKKRKDIPKIASHVFQQIIR
                     IDTQSTGLASRE"
     gene            12316..13359
                     /locus_tag="PLW95_05625"
     CDS             12316..13359
                     /locus_tag="PLW95_05625"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HOV22142.1"
                     /translation="MRQLNKYSYANARIRALLSFLLSPELFERMIAAHDLGEVLSILK
                     TTTYKEVVEKLPAEVSELLLLEKALLKDNLAACWKIYDAIIGKQEKMFIFLLIQRYEL
                     EEVKVALRIWHKKNPIDWNEYLLGETIAHQIDFSKIISSQNLDEIISILDSTPYKNPL
                     MQAREQFQKKNSIFYLEIALDIDYYKRLLECAEKLSSTDQKVTKKILGVEIDIENIRW
                     LLRQHKYYNNLNLDEMLEGVIHGGEHITRERVRSSYNSNGISNLIKGLAIGPYSQVKN
                     LVTGNVILLEKFLYEILFHEVHRVLSSFPFTMGVALGYLILKNQETKNIVSLSYAKYY
                     KIEREEISSLIFQ"
     gene            13451..15358
                     /locus_tag="PLW95_05630"
     CDS             13451..15358
                     /locus_tag="PLW95_05630"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOV22143.1"
                     /translation="MLIPSEMVLMSIVVFKEESEELTSRILNLGIFQPVDIRTIEKEL
                     EDLTPLNTEKEYAEWEKLEVQLHQVAKTLNLSIFASGKIEHFSPNMIKNFLSEVEEEV
                     SPLLNQKEEIQNNLQNIETILSQIKDYIPFPIKKSSTYTFLNIELGSIEEKNMVVLER
                     TLKNIPHLTLPLKKEMGRVVILVVYLKKDEVFLTKVLKDVSWQPMEYPEGLPEIPQEM
                     QNKLNAELEEYRNKIEEIDKEIKKLGEKYKEKFFAILGYITREKALLQAKKYAFITGR
                     TFLISGWIPKEEKNRVVKEVRSVVPSAYLEIRATKTLNVDKEQIPVRLKHSKFLKPFE
                     LLITSYGLPRYGTIDPTLFVAISFLIMFGAMFGDLGHGLVLLLGGLLLKRSKQETMKQ
                     GGSLLAWCGVSSCIFGFLYGSFFGQEKIIPAIWKNPFYNINDIFRVSIMFGIGVLTLG
                     ILLNIINSLLDKNYFRALFDKAGLISGFLYWVGIALVSKSFIAKAPVPPAYLVLLIAG
                     VCLLFFKPVLERTIAKKNESLGVSFMESIIDLLEIIMGYLANTISFIRIAAFSLAHAG
                     LFMAIFELSRLVSGIGGNILSFLVILFGNILVILLEGMVVGIQSLRLNYYEFFSRFFV
                     PSKQEFKPLTK"
     gene            15457..16548
                     /gene="mnmA"
                     /locus_tag="PLW95_05635"
     CDS             15457..16548
                     /gene="mnmA"
                     /locus_tag="PLW95_05635"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOV22144.1"
                     /translation="MKKKVVIGMSGGVDSSVAAALLKEKGYEVIGVYMNLYSGKEQEN
                     KEIICYGQKEENLKIAKTTAEILNIPLFVVDVEGEFKKKVIDYFIKEYKEGKTPNPCV
                     MCNRYIKFGILLEKLKKSDINFDYFSTGHYAKVKYNGKRYIIQKGKDKEKDQSYFLFL
                     LTQKQLSKVIFPLGDFKKTEVREIARKYKFPSYERDESQDFISGTPSFFDNEKEIEIV
                     DKRGNVLGKNKGIFYYTIGQRRGLGIAKGMPLYVIGIDAKKKGIIVGEKKDVYKRCLI
                     AGNINFVSVDKLKYGMKVKGKIRYKSPLSTAYVFPYKNDKIIVRFEKEQWAITPGQAV
                     VLYKGSTLLFGGIIEKGVSDEEITLYIKK"
     gene            16517..17779
                     /locus_tag="PLW95_05640"
     CDS             16517..17779
                     /locus_tag="PLW95_05640"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOV22145.1"
                     /translation="MKKLLCISRNKRDDFLKVLDEVINSIETGKYDETIEKVINENEG
                     KVRGCFIFDSFSELKEKIEKIKNTTEEKIIQEDIYFSITELEGKIAFLFPGQGSQKVG
                     MFSTLLEKFPQIKESIEIANETLKEKLGKNLSEFIYPQEVKSEEEKRKQLEQLTITNI
                     TQPVLGVIEMGLFKIINSFGILPDMAGGHSLGEYVALSASGIIEERFLYTLLESRGRA
                     IIEGGGEEGKMMAIGLPEREVKNLISGYEVYIANLNSPSQTIIAGRIEELEKLKDALK
                     GKKIKSKIINVSCAFHSPFMEKAKTIFDEKLKKVEFKKGKFPVYSNLTGEKYPDNMDE
                     IRNILSEHLIKEVRFIDEIENMYRDGSRVFIEVGPGNVLTGLVNSILSGKNFISITID
                     KKDEIEGLLSVLAICFLYNKHTLYKIFN"
BASE COUNT         6655 a         2396 c         3513 g         5462 t
ORIGIN      
        1 ttggtgcgtc atttccccct ttaacaaagg cctgtccgac gcgctttttg gcggaaagat
       61 aaaggtggat ttcagttaac acaacggggg ttagagagaa ttttggaggt tcaacaagca
      121 caacgggggt tagagggaat tttgaaaaaa tgtaaattgc aaaaggtatt aaaagagata
      181 agttattgat tttcagatac ttgttaactt cgtaagttaa caatggagga agagatggaa
      241 aataagggac tattatatac aaaagaacat gagtgggtaa aaattgaagg caaagttgga
      301 cttgtaggaa ttactgatta tgctcaaaat tcattaggag atattgtttt tgttgattta
      361 ccacctgttg gaagaaaagt taaacaattt gagacatgtg ggaccataga atcaataaag
      421 gttgcctctg acattttttc tcctatttcg ggtaaaatta tagaagttaa tgaaaattta
      481 aaagaaacac cagaaattat caataaagca ccttatgaaa gttggctgtt taaaatagaa
      541 gttgaagatg aaaaagagaa agaaaattta atgaatgaag ttgaatatca gcagtatata
      601 aaaaacttat aaaaatgaat tacacaccac atactgattt agaaataaaa gagatgttaa
      661 aggaaatagg tattgaaagt atagatgaat tatttaaaga tataaaacac atattacttc
      721 ctaaatcatt taatttgcca gaaggaaaat cagaaatgga tgttttaaaa tatattgaaa
      781 aactatctta taaaaattat acagacctga catgctttat tggtgctgga ttttatgacc
      841 attatattcc atcagttgtt gattcaatat cttctcttcc agcattttat accccatata
      901 caccatatca accagaagca tcacagggat ggttacaggc actttatgaa tatcaaagtg
      961 taatttccaa tttgacagat atggataccg caaatgcctc tttatatgat ggaggaactg
     1021 ctttgtatga ggcgtgtatg atgagtacaa aaataactgg caggaaaaaa attataatgg
     1081 atgctggtgt aaatctaatt tatagaacaa tgctttatag ttatactttc aatttagata
     1141 ttaattttgt tgaaatacca aatattgatg gaataacttc aagagaaaaa attataaaat
     1201 ctcttaatga gggaaatgta tcagcaataa tacttcaaaa ccctaatttc tttggatata
     1261 ttgatgatta ctctgatata gttgaaatgg cacataaagt tggaacactt gttatacttt
     1321 ctgtatatcc agtatctctt ggacttttaa agacaccagg ggaaatgaat attgatattg
     1381 ctgttggtga aggacaatct cttggactgc ctctctattt tggtggtcca tatttaggat
     1441 ttatatccac aaaaaaggac tatgtaagga aaatgccagg aagaattata ggtagaacaa
     1501 aggataaaaa taataaagat tgttatgtta tcactttaca aacaagagaa caacatgtaa
     1561 ggagagaaaa agcgacatca aatatctgta caaatgaagc actatgtgct ttaaggtcgc
     1621 ttatttattt atgtctgctt ggaaaagagg gaataaaaga actcgctcag ttaattttgg
     1681 aaaaagcatc ctttacaaaa gaattgataa aaaaggtgcc aggggtaagt atctgtaaaa
     1741 aatccctacc aacttttaat gaattcacta ttaaacttcc taaaaatgca gatgaagtcg
     1801 tgaataaaat gattgaaaaa ggatatattg ctggctttcc cctaggcagg ttttataaag
     1861 gtatggataa ttatttactt attgcaataa ctgaaaaaag aacaaaagaa gaaatagtta
     1921 aatttgtaga aagtttggag gcggtcttat gcgattgatt tttgagcagg gtagaaaagg
     1981 aagaaggggt tttaaaattc ccaaaaaaga tgttcctatt tcaaaagtag atattccaga
     2041 aaaatataaa aggaaaacgg aaattaacct accagaagtt tcagaattgg atgttgtcag
     2101 acattttaca aatttatcaa aattaaattt ttgtgttgat acaaattttt atccattggg
     2161 ttcctgcaca atgaaatata acccgaaatt cattgaaaag gcagcaaatt taaatgggtt
     2221 tagggattta catccgcttc ttcctcaact ttcaggaggt ggaagattaa ctcagggtgc
     2281 tcttgaaata ctttatagaa tagaacaact tttatgtgaa ataacaggga tggatgcttt
     2341 cactatgcag ccattagcag gagcacatgg agaactcaca ggtacaatgc ttatatctgc
     2401 ttatcacaaa gataaaggaa ataagcgaaa atatataata attcccgatt cctcacacgg
     2461 aacaaatcct gcaagtgctg caattgctgg atatgaggtt atagttgttc atacagataa
     2521 agatggcttt atggattttg atgaatttga agaaaaaatg aatgaaaatg ttgcaggagt
     2581 tatgcttaca tgcccaaata ctcttggtct atttaatcca aaaattaaag aaatatgcga
     2641 tatttctcat aaatatgatg ctttagttta ttatgatgga gcaaatttga acgctgttct
     2701 tgggaaaatg agaccagggg atattggttt tgatattgtt cacttaaact tacataaaac
     2761 atttgctact ccgcatggag gaggtggtcc aggagcaggt cctgttggag taaaaaaaca
     2821 tcttattgaa tttttgccaa tatcaagagt tattatgaaa aaagatggaa cttttatgct
     2881 tgattatgat tatccaaaaa caattggtta tattgcccca ttttatggga attttggagt
     2941 aattattaaa gcatatgcat atattttatt acttggaaaa gagggacttt tagatgtttc
     3001 tgataaggca gtactaaatg caaattattt aagagcaaaa ttgagtaaat attatgaaat
     3061 cccttatgat aaaacatgta tgcacgaatt tgtattatca gtagaagata agaaagaaaa
     3121 atatggagtt agtgcaaatg atattgcaaa atatttaata gacaaaggaa tacatccacc
     3181 aactgtttat tttccactga ttgttaaaga agcattaatg atagagccaa ctgaaacaga
     3241 aagtaaagaa acattggatg aatttattga tgttatggta gaaattgcaa aattatgtga
     3301 agaagacccg gataaaataa aaaatgcgcc ctataataca tatataagtg aacccgacga
     3361 agttaaggcg gcaaaagatt tagacctttc ttataaaaaa tgaaaagcag atacatattt
     3421 tcttcctttt cttcacctta tgagaatatg gcaatagatg aatcattgtt tatttcctat
     3481 tttgataaaa aagtgcctac atttagaatt tatggatgga gaccatatag tttttcaatt
     3541 ggatattctc aaaaagcaaa agaaattttg aatttagaaa agtgcaaaaa agacaatatt
     3601 ggtatagtaa gaaggataac aggtggaagt atgatttttc atgctgatga ggtaacttat
     3661 tcaattgttt gttcagaaag cgatattgga aatccagcaa gtataaaggc ctcatataaa
     3721 attttgactt cttttatttt aagggcatat gaaaaattgg gactttttcc aaaatatgca
     3781 atagaatttt cagatataat aaaagagaaa tcatctttct gttttatgtc tcaggaggac
     3841 tatgatatta ttataaaagg aaagaaaatt gggggcaatg cacaaaaaag aaaaaaagat
     3901 gttattttaa ttcatggttc aattccactt ggtgatgact ataaaattgc ccaaaaatat
     3961 acaaaagaaa atcttttgaa attaaacata agaagttcct gtttatcaga agttcttgaa
     4021 aggaaaattg acttctttga attttcagaa atattaaaag aaagttttaa agaaatattt
     4081 ggtgaatttg aatttgatga attagataaa aaagaaatta acttaaaaga agcattattg
     4141 aaagagaaat attcacaaga ttcatggaat ttatattaca atgaaacctg attaccttaa
     4201 aaaaagaata aatttagaag atacatctta tgttaaaaat attttgaggg acttatcatt
     4261 acatactgtt tgtgaagaag cattctgtcc gaatatttcg gaatgctttt caaaaagagt
     4321 agcaacattt ttaatattgg gtaaaatatg cacaagaaat tgtaaattct gtgatataga
     4381 aaaaggcaga ccagaagaat tagataaaga tgaaccagaa aaagttttag aagcagtaaa
     4441 aaaacttcgt ttatcctttg tagtaattac aagtgttaca agagatgatc ttgaagatgg
     4501 gggaagtgaa atttttaaaa tgtgtgttct aaaaataaaa aattttgata aaaatataaa
     4561 aatagaaaca ttgatacctg attttaaagg gaatataaaa gcacttgaaa aggttgtaag
     4621 tgtatctcca gaaataattt ctcataatat tgaaacagtc ccttcccttt attcagaaat
     4681 aagaagcaaa gcaaattata aactttctct ttcagttcta aaaaatgtta aagaactgaa
     4741 taataaaata tatacaaaat caggcattat gcttggactt ggtgaaaagg aagatgaagt
     4801 tataaaagtt atggatgact taaggcaggt ggattgtgat tttttaagta ttggacagta
     4861 tttaagcccg agtaaaaacc attatcctgt taaagaatat atctctcctg aaaaatttga
     4921 ttattataaa gaaaaagcaa tgaattctgg ctttaaatat gtaaaatcag gtccttttgt
     4981 tagaagttct tacttagcag aggaatattt gttaagtaat agtaaaatcc cttaatttta
     5041 taaatgagaa attggtgtta atatttttta tccctttact ttttttaaag aatactataa
     5101 tagaaaaaaa ttattatatc taaattttga ctattgacaa caaaattgga aaagggtaaa
     5161 ttacatatta agcataggtt taatactata aaataaaggt agttatcgaa ggtaattatt
     5221 ataggtaaag ttatatagtc taattgaact ctatatattt ttataaacac ctttaaaaac
     5281 tctaacttta tacaaggaga aaaatgaaaa gattaagaat aatttggtta ggtgcagtaa
     5341 cgatggttct ttcctgtttc catagcgttg catgggctca ggttccaggg acagatgcag
     5401 cagtgacaaa atgggtatat atttctgcgg ctcttgccac tggaattagt tcattagggg
     5461 ctgcaattgc agttgcttat gtaggcgctg ctgctgttgg agcaatgagc gagaaaccag
     5521 aactggcagg cagggcttta atttatgtag gtctggctga aggaattgct atttatggtt
     5581 taattatagc gattatgatg ttggggaaag tctgatgaga tttttttgta ttgcggacaa
     5641 agacagtagt ttgggattta gattagccgg tgtggaaaca agagaagtta ctacaaaagc
     5701 agaagtattg gaagcatttc aggtagcatt agcaactgat gagattggga ttcttgtaat
     5761 aactgaaaaa gcggctaatt ttattcgtga ggaaatagaa aattgtattt atcagaaaca
     5821 gttgcctctc attttagagg ttccctctcg tgatacagaa ggtatctccc gcgatataag
     5881 tgaatttttg agaaaggcaa ttgggataag tgtatagtaa agacaatgga tatagaaaaa
     5941 tataccgaag atacagaaca aataaaagaa ataagcagga gaattcaaga agaagcagat
     6001 tatcaggcaa gggatattat ccagcaggcg aaacaggaaa gtgaaaatat tttaaatata
     6061 gcccagcaac aaattgcttc agaaagaact aagaagatgg ctgatttgga attggagttg
     6121 aaacaaatga gggaacatat tttttcttct ttgaatttag aaaagcgccg aattgaatta
     6181 aaggagaaag aaagatttat aaagaagata ttagaagaag ttgaacttca agcaaaatcc
     6241 ttccgtactt ctgaagaata cccaaatttt ttgatgaaag atattttaga aggcatttca
     6301 gtattagata aggaggaatt gcgagtaatt tactcttctc ttgacgaagc aatatttaca
     6361 gaggcattct tacaaaaatt atcagaagaa tgtcataaac aattgggaaa aaaagtgatt
     6421 ttacacccag aaaaaggtga tttttcagat attggtgtga aattacaatc aatggatgga
     6481 cggattgttt ttgataacac tttttctgcc cggctggcgc gtatgcaaaa tgagatttat
     6541 aatcgtctgt tgaaagaaaa ttaaaaaata tctggaataa agacaaatct tttaaatttg
     6601 taatttttta aacaaggtga ggacatatga tatgtggtaa aatttttcgc gtctctggtc
     6661 cggtagtaga actggaagag gtagttaatc tccggatgat ggatatggta gaagtaggag
     6721 aagaccatct agtaggtgaa gttgtccgat tgaaaggtga caggggttat atccaggttt
     6781 atgaagatac cacttctttg aaagcagggg attttgctta tacccagggt tttccccttt
     6841 atgtggaact tggtcccggt cttattagta ctatttacga tggaattcaa agaccacttg
     6901 aagttattaa aaataaatat ggtatctata taaccagggg tgtgcatgtc agtccaattg
     6961 ataagcagaa gaaatggcat ttttatccca gtgcagtaga aataggaaaa gaaattgcat
     7021 caggatttgt tttaggagaa gtacaggaaa cacccaactt aaaacatctg attatgcttc
     7081 atcccaatct aagtggtatt ttaaaatgga ttgcacctga aggtgattat acagttgagg
     7141 aaaaaatagc agtgcttgaa aataaccacg ggaaagaaga aatttatatg tatcaccgat
     7201 ggcctgtgcg tacaccaaga cctgtcaaat cccgatttcc agtaagtaag ccgttgatta
     7261 caggacaaag agttattgat actcttttcc caattgctaa gggcggttgt gtggcagtgc
     7321 ctggtggatt tggcacaggc aaaacagtag tccagcacca gttagccaag tggagtgatg
     7381 cggatatagt tatttttgtg ggctgcggag aaagaggaaa tgaaatgata gatgttcttc
     7441 taaatttctc caaacttatt tctccaaaaa cgaaccgacc tctcttggaa agaacaattt
     7501 ttattgcaaa tacttctaat atgcctgtgg ctgcaagaga agcaagtatt tacactggaa
     7561 ttactcttgc ggaatattat agggatatgg gttatcaggt tgctctaatg gctgattcta
     7621 caagtagatg ggctgaagcg ctgcgggaac tatcaggacg actggaagaa atgcccttag
     7681 aggaaggatt tcctgcttat ctttcttctc gactggctga attttatgaa agagcaggga
     7741 attttcagac actttcaggt aacaatggtt ctatcaccat tatagcatca atttctccac
     7801 caggtggtga cttttcagag cccgtaactt cccataccaa acggtttgtc agaagtttct
     7861 gggaacttga tagagaattg gctaacgcac gacattatcc atccataagt tggattgaga
     7921 gttattcaga atacttagat gatgttaagg agtggtggca taaaaatatt gacccacaat
     7981 gggaaacagc acgttatgcc ataatggacc ttctacaaaa agaacaaaaa ctacttcaag
     8041 tggtaaaact ggtgggtcct gatgttctgc ctcaaacaca gcgtttgata ctggatatat
     8101 gttctatttt caagagtgcg tttcttcaac agagtgcttt tgataaagta gatacatata
     8161 cttcgccaaa aaaacagttt ttaatgctaa atgccatatt gaagttatat gagaaaagtg
     8221 aggaattggt aaagaaaggt gttaccattg ccgagattaa agcaacggat gtttatgagg
     8281 atattttaag aatgaaaacc aaatatactg aggaagatat agaaaaatta gaggaattac
     8341 ctgtccggat aaatacagag ttagatgaac ttaatttttg aatataaaaa ttagttatta
     8401 gtaaaacata tgaaggttaa caggtatata gtccaaaaaa tattagagaa aaggcaagaa
     8461 atttgacaca atatctcaaa agttataaag ttaaattata gaattagtca gagataagaa
     8521 agtttaatat ctatgatttt gttgagtagt gtcaaaaact tagatgaaag agagagagaa
     8581 aataaaaatc cccttcaaaa tccccctccc ccctttggtt aaagggggaa attactcctt
     8641 cctttgtaaa aggaaggtag ggatggattt ctacttttca catttcattt gtttttgtga
     8701 gaaagtgtgg agtgaattta attaaccaat tttgacagaa ccttttgttg aaagaggata
     8761 ttttatgaca gaaaaatatt cactgcggga atacatggga ttggaagaaa tagtaggtcc
     8821 tatttttatc atacgagata tccacaatgt tggttataac gaactggtag agattgtaga
     8881 taatcaagga aaaagtcgca ttggtattac ccttgaagta ggtaagggct atgcagtggt
     8941 agaagtactt ggtggaactg tgggcctttc tttaaatgag tcccgtgtgc gttttaaagg
     9001 agagccatta atgttaggcg tctcagaaga aatgttaggc aggatatttg atgggcttgg
     9061 caatccttta gatggatttc cacgacctgc ctatgaagag atgatgaatg taaatggtca
     9121 ggctattaat cctactgcca gagattatcc tagaaacatt attgagacag gactttctgc
     9181 aatagatgtt atgaatactc tggtgcgagg gcaaaaacta ccaatttttt ctggaagtgg
     9241 tctacctcat gatttaattg cagcacaaat tacccgacag gcaagagtaa aagaagaaca
     9301 attttgcgtt atttttgcgg ctatgggtat aaaatatgac acagcacaat ttttcataag
     9361 aaattttgaa gaaacaggag ttttggaacg aactgttatt tttttaagtt tagcagatag
     9421 tccctcaata gagcgacttc aaacccctcg tctggcttta tcctgtgcag aatttcttgc
     9481 ttttaaaaag catatgcatg ttctggtaat tatgactgat atgtcaaatt attgcgaagc
     9541 acttcgggaa gtttctacga taagaggtga aattcctgcg cgtaaaggat atcctggata
     9601 tctatacagc gatttagcaa gtctttatga gcgagcagga ataataaaag ggtcagaagg
     9661 ttctattaca caattaccta ttctaactat gcccaatgac gacattactc atccaatacc
     9721 tgatttgact ggttatatta ccgaaggaca aattgttctt gaaagggaac tttttagtag
     9781 aggtatttac ccgccaattg cgggattacc ttcactttcc cgtcttatga aagataatat
     9841 cggtgccggg atgaccagag aagaccatcc agacctatct tctcaattat ttgcctgcta
     9901 tgcacatgtg aaagatatca gagctctggc tgctattatt ggggaagaag aactttctcc
     9961 gttagaccaa ttgtatctca aatttggaga tgcttttgag cagaaattcc tcagtcaggg
    10021 acattatgaa agacgaacac tggaacaagg acttaatttg ggctgggaaa ttctttcaga
    10081 actaccagca gatgaacttc ttcgtgttaa agaagaagaa ataaaaaaat attacaaagg
    10141 tgaaaaatga gaatacaatg tcctgctact aagacaaatc tccttaagat gaaaaaagcc
    10201 cttttactta cagaagaggg ttatcaactt cttgaggaaa aaagaaaaat tattatgaat
    10261 gcactgactg gtctaattca tataatacac cagtcagaag aagaagttgc tactgctctt
    10321 aaagaagggt ataatttggt tgataagaca atatgttcta tgggcagaaa gaaattagaa
    10381 gaaataagtt tctctataaa cattcagaat agtatatcta tatctcacag gaaggtaatg
    10441 ggtgtttctt tacctgatat caaacttgat ttgcaggata atcctcctta ttatagtcca
    10501 tatgaagtaa gtttatatac tgatgaggta atagagcatt ttaagaaaat tctttctctt
    10561 ttagttgagt tggctgagaa gagaattgct cttttacgac ttgctaaaga gatgcaaaaa
    10621 acaatgcgta aggtcaatgc acttgagaaa gtatatattc ctgcttacag ggaaacagtt
    10681 aagtatattg gagatagatt ggatgaagaa agtcgggatg cattttcctt attgaaattg
    10741 ataaagcaaa ggcacgcagg taacagagtt acctaagaag tagtgagtag taagtaaaaa
    10801 tataaaataa aaaagaaatg gttaaaattt tactctctac tttcttcttg ctacttaaac
    10861 ataaacagag tgcagtaaat attttcggag cgaatgccta tggctataaa gaatatactt
    10921 gtttctcttt cggtagatat taactcatct cttgttactg caaaatatgc tatttatata
    10981 gcaaaagttc tacaagcaaa attgattgct gtatatgtag ttgataaaga agcattacag
    11041 aaattgataa aagccagaat attcatagat actgaagcga tggaatacga acgggatata
    11101 gaaaatgagg gtaaaatatt ccttgataga tttaaacaga tggcagaaaa caaagaagtt
    11161 ccttttgagg gaatagaatt aaagggcgta gttcataccg aagtgataaa taaaatcaaa
    11221 gaaacaaatg ccgatttgct tattatggga gaactgaaag aagctctttc tctgagggat
    11281 actttttata acgaaggcgc cctgattttc tttgaagcac cctgtccggt tctggtggtg
    11341 aaaaacaaaa aatatgtaga actattatac caaagcattt aaccatgatg taaattggga
    11401 attttttggt aaaaagttct gtataattat catgaccctg ttttcttttt tatgacatgc
    11461 tcataaaaag agaattaaat ttagaggaga cctatgaaag aagttatctt agaagtactt
    11521 aaagaagaga agtgtgcaga agagaaattg gaaagtgcgc gcaaagaggc cgaaaatatt
    11581 gtgttagaag caaaaaaaaa ggcgcaattc cttattgaag aatccagccg aaaaaccaat
    11641 gctgaggtta ttaagaaaaa ggcagaaatg gaacaagcat atctggaaga aaaagaaaac
    11701 atactacgga ccgtacagaa agaaactgat gcctacttgc agaaaaaaag aaaagatata
    11761 ccaaaaatag caagtcatgt ttttcagcaa attatcagga tagatactca gtcaactggg
    11821 ttggcgagta gagagtagca agaataaaaa caaaaaaagt taaatctatc ctaataaacc
    11881 gactaaggtt gcagaggggt agcaaagcag agttggtgcg ttattccccc cttttagttc
    11941 tcccctttaa caaaccaact aaagaggcgg ggattagcaa agcagagttg gtgccgtaat
    12001 gtatttcaat aggcacaacg ggagtgagag gggatttaat aaaatccacc cccaccttct
    12061 cctccactaa gacattgacg gacatgcttt ttcaaaggaa ggagacaaac gttccccctt
    12121 tagtaaaccg actaaagtag cggggattag cagggcagag ttggtgcgtc atcgtctccc
    12181 agatagcaca acaggggcga ggggagattt aataagcaca agggggggtt caaagaggag
    12241 atgtaggact ttctacttgc tactaagata taaatgggga atttgtaata tttacttatt
    12301 ttatttatca taaaaatgcg acaacttaat aagtattcgt atgctaatgc aagaatcagg
    12361 gcattgcttt cttttttgct ttcgccagag ttgtttgaac gaatgatagc agcccatgat
    12421 ttaggtgaag ttctaagtat tttgaaaacc acaacttaca aggaagtagt tgaaaaacta
    12481 ccagcagaag ttagtgaact tttgcttctg gaaaaggcat tgttgaaaga taatttagcc
    12541 gcctgttgga agatttatga tgctatcatc gggaaacagg aaaaaatgtt tatttttctc
    12601 cttatccaaa gatatgaatt ggaagaagta aaagtggctc tgcgcatctg gcataaaaag
    12661 aacccgattg attggaatga atatctatta ggagaaacaa ttgctcacca gatagatttt
    12721 tctaaaatta tttccagtca aaatcttgac gaaattatct caattcttga tagcacacct
    12781 tacaaaaacc cccttatgca ggcacgggag caatttcaga aaaaaaattc catattttac
    12841 cttgaaatag ccctggatat tgattattac aagagacttc tggagtgtgc agaaaaacta
    12901 tcttctactg accagaaagt gactaaaaaa attttagggg tagaaattga tatagaaaac
    12961 atccgttggc ttttgcgtca gcataaatac tacaacaatc ttaatttaga tgaaatgctg
    13021 gaaggggtaa tacatggggg tgagcatatt actcgagaaa gagtacgcag ttcctataac
    13081 tcaaacggaa tttctaattt aataaaaggt ttggcaatag gaccttacag tcaggttaaa
    13141 aatctggtca ctggtaatgt tattcttctg gagaaatttc tttatgagat tcttttccat
    13201 gaagtacacc gagtactttc aagttttcca tttacaatgg gagttgcttt aggttattta
    13261 attttgaaaa atcaggaaac caaaaatatt gtttctttat cttatgcaaa gtattataaa
    13321 atagagaggg aagaaatctc ctctctgatt ttccagtaag atagtattat gagaagtaga
    13381 gagtagccct ttggactact cacaaataaa atatgaaagg gactaaatac aatacttaaa
    13441 aagaggaatt atgttaatac cttcagaaat ggtactaatg agtatagtag tttttaaaga
    13501 agaatctgag gaattgactt ccaggatttt gaatttagga atttttcaac cagtagatat
    13561 ccgaacaatt gaaaaagaac tggaggactt aacacctctg aatacggaaa aagaatatgc
    13621 ggaatgggaa aaattagaag ttcaactgca tcaggtagca aaaacactta atctttctat
    13681 ctttgcatcc ggaaaaattg aacatttttc tccaaacatg ataaaaaatt tcctcagtga
    13741 ggtagaagaa gaagtcagcc cacttctcaa tcaaaaagaa gaaattcaga acaatttaca
    13801 gaatatagag acaattcttt ctcagataaa agattatata ccttttccca taaaaaaaag
    13861 ttccacctat acttttttaa atattgaact cggaagtatt gaagagaaaa acatggtagt
    13921 tctggagcgt acattgaaaa acattcccca tctaacgctg cctttgaaaa aagaaatggg
    13981 acgggtggtt attctggttg tatatctgaa aaaagatgag gtatttctta caaaagtatt
    14041 aaaagatgtt tcatggcaac caatggaata tccagaaggt ctaccagaaa ttccccagga
    14101 gatgcagaat aaattgaatg ccgaactgga agaataccgt aataaaattg aagagataga
    14161 taaagaaata aaaaaattgg gagaaaagta taaagaaaaa ttttttgcta ttcttggcta
    14221 tattacccgg gaaaaagcac ttctccaggc aaaaaaatac gcattcatta ctggtagaac
    14281 atttttaatt tcaggatgga ttcccaaaga agaaaaaaac cgggtggtaa aagaagtcag
    14341 aagtgttgta ccttctgcat atctggaaat aagagcaacc aaaaccttaa atgttgataa
    14401 agagcaaata ccagtgcgtc tgaaacatag taaatttctt aagccatttg agttattaat
    14461 tacttcttat ggactacctc gttatggaac aattgacccc acattatttg tagccatcag
    14521 ttttctaatt atgtttgggg ctatgtttgg cgatttagga catggattgg ttcttttgct
    14581 tggtggactt ttactaaaaa gaagtaaaca ggaaactatg aagcaaggtg gttcccttct
    14641 tgcctggtgt ggagttagtt cctgcatctt tggttttttg tatggaagtt tttttgggca
    14701 ggaaaaaatt attccagcaa tctggaaaaa ccctttctat aatattaacg atattttccg
    14761 cgtaagtatt atgtttggta ttggtgtgtt aacactggga attttgttga atataatcaa
    14821 ctctttactg gataaaaatt atttcagagc actttttgat aaagcaggtc taatttctgg
    14881 cttcttatat tgggtaggta tcgcactggt aagcaaatcg ttcattgcca aagcaccagt
    14941 tccacctgca tatcttgtat tgctgattgc tggagtttgt ctacttttct tcaagccagt
    15001 tttagaaaga actatagcaa aaaagaatga atcgttaggg gtatctttta tggaaagtat
    15061 aatagaccta ttagaaataa ttatgggcta tttagcaaat actatttctt ttattcgtat
    15121 tgcggctttt tccttagcgc atgcaggatt atttatggca atttttgaac tatcccgtct
    15181 cgtttctggt attggcggta atatactttc ttttctagta attttgtttg gaaatattct
    15241 tgtaatcctt ttagaaggaa tggtagtggg catacaatcg ctacgattaa attattatga
    15301 atttttctcc aggttttttg taccaagtaa acaggaattc aagccattga caaagtaatt
    15361 tctaacttct aattttctaa aaaaagatga atattgactc aattatatat tactaaaaaa
    15421 aagtaattat cataaatttt ttccactttt aaaaaaatga aaaaaaaagt tgttatcggg
    15481 atgtctggtg gagttgattc atctgttgca gcagcattat tgaaagaaaa gggatacgaa
    15541 gtaattggtg tttatatgaa tttatatagt ggaaaagaac aggaaaataa agaaataatt
    15601 tgttatggac aaaaggaaga gaacttaaaa attgcaaaaa caacagcaga aattttgaat
    15661 atacctcttt ttgttgtaga tgtagaagga gagtttaaaa aaaaggttat tgattatttt
    15721 ataaaggaat ataaagaagg taagacccca aatccctgtg taatgtgcaa tagatatata
    15781 aaatttggaa tactgcttga aaaattaaaa aaatcggata taaattttga ttatttttca
    15841 acaggacatt atgcaaaagt aaaatataat ggaaaaagat atattatcca aaaaggtaaa
    15901 gataaagaaa aggaccagag ttatttcctt tttcttctta ctcagaagca actatcaaaa
    15961 gttattttcc ccttaggaga ttttaaaaaa acagaagtca gagaaattgc cagaaaatat
    16021 aagttccctt cttatgaaag agatgaaagt caggacttta tttcagggac accttctttt
    16081 tttgataatg aaaaagaaat agaaattgtt gataaaagag gaaatgttct tggtaaaaat
    16141 aaaggaattt tttattatac aattggacaa agaagaggac ttggaattgc aaaaggaatg
    16201 cctctttatg tcattggaat agatgctaaa aaaaaaggaa taattgtagg agaaaaaaaa
    16261 gatgtttata agaggtgttt aattgcagga aatataaatt ttgtttctgt tgataaattg
    16321 aaatatggaa tgaaagttaa aggcaaaata agatataaaa gtcctttatc aactgcctat
    16381 gttttcccat ataaaaatga taaaataatt gtcagatttg aaaaggaaca atgggcaata
    16441 actcccgggc aggcagttgt tttatataaa ggaagcacat tattatttgg tggaattata
    16501 gagaaaggag ttagtgatga agaaattact ttgtatatca agaaataaaa gagatgattt
    16561 tttaaaagta cttgatgaag ttatcaatag cattgagaca ggaaagtatg atgaaacaat
    16621 tgaaaaagta ataaatgaaa atgaaggtaa agtaagagga tgttttatat ttgattcctt
    16681 ttctgaatta aaagagaaaa ttgaaaaaat taaaaataca acagaagaaa aaataattca
    16741 agaagatata tacttttcaa taactgaatt agaaggtaaa attgcctttt tattccctgg
    16801 ccagggttca caaaaagttg ggatgttttc tacacttttg gaaaaattcc ctcagataaa
    16861 agagtccatt gaaattgcaa atgaaacctt aaaagagaaa ttggggaaaa atttaagtga
    16921 atttatttat ccccaagaag ttaaaagcga ggaagaaaaa aggaaacaat tagaacaact
    16981 tacaataaca aatataactc aaccagttct tggtgttatt gaaatggggc tttttaaaat
    17041 aattaattca tttggtattt taccagacat ggcaggtgga catagtttag gagaatatgt
    17101 tgctctttct gcatcaggaa taattgagga aaggttcctt tatacacttt tagaaagcag
    17161 agggagagca ataattgaag gtggaggaga agaaggaaaa atgatggcta ttggattgcc
    17221 tgaaagggaa gtaaaaaatt taataagtgg atatgaagta tatattgcaa acttaaattc
    17281 accttctcaa acaataattg ctggtagaat agaagaactt gaaaaattaa aagatgcctt
    17341 aaaagggaaa aaaataaaaa gcaaaattat aaatgtaagt tgtgcttttc attctccatt
    17401 tatggaaaaa gcaaaaacaa tttttgatga gaaattaaag aaagttgaat ttaaaaaagg
    17461 aaaattccct gtttattcaa acttaacagg ggaaaaatat cctgataaca tggatgaaat
    17521 aagaaatata ttaagtgaac atttaataaa agaagtccga tttattgatg aaattgaaaa
    17581 tatgtataga gatggaagta gggtctttat tgaagttggt ccgggtaatg ttttaactgg
    17641 tcttgttaac tcaattcttt caggcaaaaa tttcatttca attacaattg ataaaaaaga
    17701 cgaaattgaa ggacttttat cagttcttgc tatttgtttt ctttataata aacatacctt
    17761 atataagata ttcaattaaa tggattaact gaaaatcaca caggcaaagt tagatatgtc
    17821 ttgacagaaa aaaagaaagg ggtaaaataa taaaaaagag aaataatgaa aagaatagga
    17881 tgtagtaata agagggaaga agtaatagtg aagagaagga attctctctc tctgcgagta
    17941 tctttttgtt tttcccttat aatcacaatt ccttttttaa taatcacaaa attttctttt
    18001 ataaattcaa ttattttatc aggagt
//
LOCUS       DAOOVW010000033        17902 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_2156_length_17902_cov_7.961002, whole genome shotgun sequence.
ACCESSION   DAOOVW010000033 DAOOVW010000000
VERSION     DAOOVW010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17902
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2156_length_17902_cov_7.961002"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="PLW95_05645"
     CDS             complement(<1..626)
                     /locus_tag="PLW95_05645"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042044.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOV22146.1"
                     /translation="MLKKFDPEIYKALVGETKKQRETINLIPSENIVSKNVLKVVGSV
                     FTNKYAEGYPGHRYYGGCKFVDIVETIAIERAKKLFHSQHANVQPHSGSQANMSVYFS
                     VLKPGDKILAMNIKDGGHLTHGTPMNFSGKLYNVKFYGVNKDTELIDYDNVREIALRE
                     KVKMIVCGSSSYSRIIDFDKFYQIAKEIGAYLLADIAHIAGLVIAGIHR"
     gene            complement(610..2691)
                     /gene="glyS"
                     /locus_tag="PLW95_05650"
     CDS             complement(610..2691)
                     /gene="glyS"
                     /locus_tag="PLW95_05650"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOV22147.1"
                     /translation="MSYFIIEIGCEDLPEWIGEYFKENFIPVFTREIKEKRINFEEIS
                     LFYTNRRIILYGKNVSPFQNDYIAEISGPPADIGIDKNGKYTLPAIKFAESQNVKVNQ
                     LKIKEKNNKKVLVAIKKEKGNKTYKLLPDIFLTSLKKIEIPRSMNWNEENIKFIRPIR
                     SILSLFDNKTIPIKYGNIKSSNFTSGHRVLSPKKIKIKHIKEYPDKMLKNFVIFDQNV
                     REKFIEEKIKNSLPFQCRYDENLVKNISNLVEYPILKIGEISEKYKGIPDRVKEVVIK
                     KLKCIPLFTSDSSLYNKFIIVLDGVDKEEIKTNYESVIINKLEDANFFIKMDTQKPLK
                     LYLDDLKKIVFHPKWGSIYERVERLQKIFECLKEKLDISETEEKNIKEIISLCKNDLA
                     TLMVTEFPELQGVIGRIYAEKEGYEKVISLSIEEHYLPEFTGDKIPEFKEGCIVSIID
                     KLETLVCFIIEGIEPTASTDPYGLKKMANGIIEIIWKKELNFPLKESIKEALFVFNNN
                     EEKIVEKILNFFLQRVDNLLAVEDIIPGIRKSVISVEKENLLTLRKKIDALKEFLKTE
                     KGIDVFVPFVRIANILKQAGERKISFGQFDENLLVEDEEKKLYEFYKKQKEKYLKLNE
                     ENNFSALLNTFAEWRDIVDNFFDEVLVMSPDEKLRNNRLSLLNKINQLFSHFADFSYL
                     SLKEIEDAKKI"
     gene            3002..3940
                     /locus_tag="PLW95_05655"
     CDS             3002..3940
                     /locus_tag="PLW95_05655"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOV22148.1"
                     /translation="MLAEVAGIPQIKLHFDVEAICNAYEKIKPIAKRLDVEAPVPRIA
                     GFCYPHIASLGAKIVFPEDGEPKPFPIIKSPEEIDNLTEPKDYLNAPIIQERLRVCYE
                     IKKRYPKSPNFIGHLFEGPITTAVLIMGSGFLTLPYDDPEKAHKLLNFSVKSALNYAN
                     TLCNYFGEPIQPGSKGLPDDFAGMFPPKIFKEFVAPYWEKIYQGNKATERYLHSELLR
                     LEHLPFLKELNIEKFDPSADQYLNPELLSKYCPCKFTSRILPWEIRDLSEDELVELYK
                     ERAKFKPSCISFYLGCLKDEPKIKCLLKIARELKEK"
     gene            3958..5043
                     /locus_tag="PLW95_05660"
     CDS             3958..5043
                     /locus_tag="PLW95_05660"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HOV22149.1"
                     /translation="MDKIKFDDLLYMRKGDKNLTMGQSPSKIEKIITVEEWEVKAKAL
                     REIFLQMLGEIPQISCPLSSKIIEENNCGDYIKRKIEYFVEPDEKISAYVLIPKNLKT
                     KVPAVLCIHPTTPLGKEQTIGNDNTEKGQDRAYALHLVKRGYITMSYDLLTAGERCYK
                     GYAPFDTAPFYKKYPKWSVRGKDLWDVSRAIDFLQSMDEVDSERIGSIGHSQGGGITI
                     HSMVLDKRIKVGISSCGMWPERLSKNPFNWARTGWWVGIPFLRPYCYTGKHFPLDMHE
                     YLGLIAPRAIMNISALNDCMYTIEEENITRGAFNNLSENVSKVFSLYKTENNFKNILH
                     MNGHSFLLEQREIAYAFLDKRLRGNNI"
     gene            5152..6327
                     /locus_tag="PLW95_05665"
     CDS             5152..6327
                     /locus_tag="PLW95_05665"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOV22150.1"
                     /translation="MEKIALLNAKIIDGKNSIPISSCSVLLNNSFIEDIIVNKKISIG
                     ENYRKIDVKNNTLLPLFIDGHMHISGEPGRLDHFGHIKANLGAVGKLQECLLWGTGVV
                     AHAAGSIENVILRDLINSGSILGCSEMLVGGAITPTCGHVRGKSADGPWEIRKAVREM
                     IGANVDFIKTTASGGFQWEHEKLTHTDYTYEELKVLVEQTHSREKKVHVHAHADPGIS
                     NAISAGCDVILHGVLINDICLEKIVEKGLWYMPTLYITSEKVFENPNLPIYMRERMKE
                     VNPFHREAVGKALKIGVKIITGTDGGPGSIMYEIYELVKCGFSPMEAIITATSKTAEA
                     FGILNRTGTIEKGKNADLMVIEGDPLTDISILCKKESILMITKKGKIIFNQFEQVKK"
     gene            6331..7986
                     /locus_tag="PLW95_05670"
     CDS             6331..7986
                     /locus_tag="PLW95_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22151.1"
                     /translation="MENSKNSLVRLIVKGVDKCKSNFDKKTGRFLAENGGWAVTNQDV
                     IFPLSYLYVNKHPENPYYKNDEILNLCFCGGDALRNAQDADGKVEFVKIDGSKWGKTY
                     MCWSMFHWLETFRLLKNFLPSERANSWQEGLYIAFEGIFKELQKSKVHNIPTWNAMAI
                     FKAGLTFQNEKWQKYAKEFIKKVVKSMNPDGYWQEGQGPTTSYNLVYVHSLGLYYCAS
                     KDEFVIPSLKTATNFHIHFTYPDGSLVETIDGRVKYNSNPSCMGLPGFLTTEKGKRFS
                     AFLVKNLCLKNPKGLCSPYLTSALEIWPDEIKPKDKIIQELTEYSRVYNGNAIVRKHK
                     NFYTCLSGYVAPAKIQSELSNNRWIQDRSNFVSIWYKLCGLIIGGGNSKEQPTWANFS
                     LWKGTFRLWCPDASQVFSKENEDKVILDFAGTKCSISVRFSGEKQVKIMFEVIKFSGA
                     DKIQSSLLLCLEKGTRVKCGKYKFIVDPIYSEVIFVKKSQTIQTKHYLIKSLKKHTFF
                     YWPEYPFNPYAINGASPPESARGKLIFELTSKSPKNEILIRVK"
     gene            8028..9854
                     /locus_tag="PLW95_05675"
     CDS             8028..9854
                     /locus_tag="PLW95_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22152.1"
                     /translation="MDKLKSYPLDKKVKELFERQRQFSKKWESTRITKEIYIELSESI
                     VRVAVSWQDKGGRIIDPYKKEETSTATARFVGALGFLIRTGHCLDLIDICIKSMNRAC
                     EDLYYSFERPHISGPEFYVKECMQGYLALKDKVDKKLVKKWEKYLGEYDPEKNYVAVL
                     SKNKPEDIHNFCTFGLAGESFKKAYKIADNSDFIERHLETQIQFFTEYGMYRDPNDPM
                     TYDWTARMNLSLLEYFGYKGKYSKDLNEILRRGGLTTLLFLSTTGESPFGGRSNQYHF
                     NEATISLICEYEANRYKRQGELQIAGAFKRTARLTALSIKRWLGLSPFRHIKNSFPPE
                     IQHGWQRGYGFFSAYSLLIASQFGFAYLLADDSIEERQTLYEIGGYILNLPDAFHKVF
                     ATCCGYHIEIDTKADNNYDATGLGRIHKISIPTELGLSTPIASKPNYSVSVEPSLRNV
                     SIGPGWEDKVGNKYYLSDLSDKIEDVQVKIIKEETEEVKFEVIYKGKFENRIKEAYTI
                     NKEGIEIEDEISGSVKDILVQIPLLQTDGENISDIKIGNKKFEVKYLNCIYKVECLDS
                     GVVDTFVEPFSAPNRNGIYKVGCFKTKGNFIKYKLSLEKCKK"
     gene            9871..10914
                     /locus_tag="PLW95_05680"
     CDS             9871..10914
                     /locus_tag="PLW95_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010094792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOV22153.1"
                     /translation="MNKKILENLRKGVFIPAHPLALTKERKLDEKRQKGLTKYYIESG
                     VGGIAVGVHTTQFEIHNPKVGLYKPVLQLAIETIKEYKLEDEIIKVAGICGKTEQATK
                     EGEIARDMGYDAGLLNLGTFTDEKDDDILIEHTKEVSRIIPVFGFYLQPATGGRELSF
                     SFWCKFFQIDNVIAVKVAPFDRYRTIDVIRAIVETGREKDIAVYTGNDDNIIIDLITP
                     FKFNNKIIRIVGGLLGHWAFWTKKSVDIFNEIKEIIKENSPIPQEILTLSAAITDVNS
                     AVFDANNKFSGCIPGINEILRRSGLLEGNWCINQDITLSPGQKEEIDRIFSEYSYLRD
                     DEFVKKNLNKWIK"
     gene            10902..11972
                     /locus_tag="PLW95_05685"
     CDS             10902..11972
                     /locus_tag="PLW95_05685"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22154.1"
                     /translation="MDKIEELKKLYEDRNLRREIEKRIKEIIGIDLSAKYGNFYLKNP
                     LIVAPGQLTRTQTQIRQIREANFGGCVLKSVVGEDKEGNCSMELYRTPPTYLKSIYEN
                     YDIDKKFPIIHWDGRGDTRNLDEYLYFAEDVFKYEKDNEFLIISSIIAHFPLPDEEIK
                     KDEWIYTTEKLYNVGARIFEIDFCPQLEKEEEKIEKENILRWYKNIPSIIKSVKKDIS
                     VFPKIMNLEFGIDFQIEMVKAAVEGKADGVVIANRIFKKEFGCAHGGKELKERNIQQI
                     KEVKKLFPDVHISATGGIYTGKDILDYIQVGAENVQLLSFLMGKSSINFGIEGTKFEK
                     VFYFLLFDSETGLLPLLKINVF"
     gene            12122..13138
                     /locus_tag="PLW95_05690"
     CDS             12122..13138
                     /locus_tag="PLW95_05690"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOV22155.1"
                     /translation="MEEKIKVGVIGLRMGLSHIEGYRRNPYTEVYGICDTDEKLLEKT
                     KQEYNIKIAVNDYKELINIPEISIISIASPDFFHCQQAIYALKNGKNVLCEKPMTPTI
                     EEAKEIVKVVKETGKKFMVGQVCRYAPGFVLAKKMVENGDIGELFFVESEYAHDYSKT
                     TGIGNWRKDKRREPFLGGGCHAVDLLRWIAGDPIEVSAYSNHKCLVDWPVDDCTIAIY
                     KFPDGVIGKVFVSIGCIRPYTMRSVFYGKEGTIICDNTSPQIYLCSKKYYREKSNFNF
                     ASFPVNLASHNVSAEIDEFVDCIINNKEVPTNEIEGAKTVISCLKAVESAKKGKPLKI
                     GGEL"
     gene            13135..14568
                     /locus_tag="PLW95_05695"
     CDS             13135..14568
                     /locus_tag="PLW95_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22156.1"
                     /translation="MKAGFFQLDITPFIGMERPSGYRKAYADFIHDPTKVRSCIIEDG
                     NEEVAIVVSDTLVSPPRENVIEIKEEIEKKVGIKKENILIGATHTHSAGPLFFPFNYQ
                     DADPLTKKLISEYSTISDPFYYEIFKRKVVDSLICAEKKKEDVICNVGYGYEDKVSFN
                     RRFKMKNNRVYTHPGKNNPDIIEPAGPIDHSVGVLTFWNKDKSPIGTFINFACHLTTG
                     PGGISADWLFYTEKIIKKIFEDMDIILLQGASGDITQVNNLSDRVDFGEKISHFVGTR
                     VGLEALKIILTEEKGSLSPLKIKNKIINVKRRKQSEEKLKMAREIVENGLKNENIRQT
                     TEWTFAKERIILDYLTKKQPEVEIEIQAIQIGPVIIISSPGEIFSSTGLKVKKASHFP
                     ITFFVELSNGFLGYIPDRKAFLPTGGGFETVLTSESNLEVDAEEKIIEESLKLMKEFK
                     PGELPEYDKITSKPQVWSYGILGPDID"
     gene            14570..15343
                     /locus_tag="PLW95_05700"
     CDS             14570..15343
                     /locus_tag="PLW95_05700"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOV22157.1"
                     /translation="MEIIDIHVHPRQDKELKEVENLINFAKKLNITRINFLGDVLYYG
                     YHPNNEQIKKINDLTIKLVKKYPCFFSGFCFVNPEHDKKFIKQEIERCIVKGNLKGIK
                     LEASCNASDKRVFKVAEIAEKLNVPILHHAWDTTNINSRWGPEVCQSDPEDIAILAKK
                     FPKVKIIMAHLIACGVRGILEIKNFDNVWAEISGGQPVTGIMEYAVKKMGAERILFGS
                     DCPGRDFSAQLSKVYGAKITAREKELILGINAKNLLGLK"
     gene            15340..16095
                     /locus_tag="PLW95_05705"
     CDS             15340..16095
                     /locus_tag="PLW95_05705"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOV22158.1"
                     /translation="MIIDININWGNWPFGNLYFNSFRNYINKIKNKGIEEGFISPIDG
                     IFSPDPDILNEKVIKKLNKQNNFKCLPIINPKIKNWQEILEKYNKEYKIKIIKIVPSF
                     HRYSIIEKDVYKFFEKLCQLDMSPVIQKRFEDERSKSPVLNLKDIEIKQIEKISKDFP
                     ELKIIILCLYWHEVIKLSEKCKNLYFDISFIECLDTLGALLKYISEDRVIFGSNVPFL
                     YPESSILKLQYSTITKEQFDKISFKNLSSFLAK"
     gene            16151..17119
                     /locus_tag="PLW95_05710"
     CDS             16151..17119
                     /locus_tag="PLW95_05710"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOV22159.1"
                     /translation="MKVKISFIGCGGIAGAHMSALSKIEDVEFVAFCDVVEEKAKQKA
                     QIYGGKVFTDYKKMLDEEKVDACYICIPPFAHDGQEELCIEKGIPFFVEKPVHLVLEK
                     CKEIAKKVEKKNLITGVGYVLRNYDVVNKAKEVIKNEKIGLVRGKYFGQVPGAEGSWL
                     HKKELSGGQLIEQATHTVDMMRYLIGDVEEVFAYKFEGINNKIYKDYNVEDASIVAMK
                     FKNGVIGNLSCTWLWSGWYSGVEIVGKGVMLTYEGNSLTIDKGNKKEIYISQEDPMLV
                     EDKNFIEGVKTGNSKIIKSNYQDAVKTLEVTLKSHESIEKGVPVKI"
BASE COUNT         6662 a         2183 c         3150 g         5907 t
ORIGIN      
        1 cgatgaatac cagcaattac aagacctgca atatgagcaa tatctgcaag taagtatgca
       61 ccaatttctt ttgcaatttg atagaattta tcaaaatcaa taattcttga ataagaacta
      121 cttccacata caatcatttt tactttttcc cttaaagcaa tttctcttac attatcataa
      181 tcaatcaatt ccgtatcttt attaactcca taaaatttta cattataaag ttttcctgaa
      241 aagttcatag gagtcccatg ggtgagatga cctccatctt ttatattcat cgcaaggatt
      301 ttatcacctg gttttaaaac agaaaaataa acagacatat ttgcctgact tcctgaatgt
      361 ggttgaacat tagcatgttg ggagtgaaat agtttttttg ctctttctat tgcaattgtt
      421 tcaacaatat caacaaattt acatccccca taatatctgt gtccgggata tccttctgca
      481 tatttattgg taaaaacaga accaacaact tttagaacat ttttactaac aatattctca
      541 gaaggaatta aatttattgt ttctctttgt ttttttgttt ctccaaccag cgctttataa
      601 atttcagggt caaatttttt tagcatcttc aatctccttt aatgataaat atgaaaaatc
      661 agcaaaatga ctgaacaatt gatttatttt atttaaaagt gaaagtcggt tatttcttaa
      721 tttttcatca ggactcataa caagaacttc atcaaaaaaa ttatcaacta tatctctcca
      781 ctcagcaaat gtattcaata aagcagaaaa attattttct tcattcaatt ttaaatattt
      841 ctctttttgt tttttataaa attcataaag ttttttttct tcatcttcaa caagtaaatt
      901 ctcatcaaat tgtccaaaac taattttcct ttctcctgct tgttttaaaa tatttgcaat
      961 tcttacaaat gggacaaaaa catcaatccc tttttctgtt tttaaaaatt cctttaatgc
     1021 atctattttt tttctcaaag tcaataaatt ttctttttca actgaaataa ctgattttct
     1081 tattcctggt ataatatctt caactgcaag aaggttatcc acccgttgta aaaagaaatt
     1141 taagattttt tctactattt tttcttcatt gttattaaaa acaaaaagag cttcttttat
     1201 actttctttt aatgggaaat ttaattcttt tttccatatt atctctatta taccgtttgc
     1261 catttttttt agtccatatg ggtcagtact tgcagttggt tcaattcctt caattataaa
     1321 acatacaagt gtttctaact tatcaatgat tgaaacaata catccttctt taaattcagg
     1381 gattttatct cctgtaaatt ctggtaaata atgttcttct atagaaagag aaataacttt
     1441 ttcatagccc tctttttcag cataaattct cccaataact ccctgtaatt cgggaaactc
     1501 tgtaaccatt aaagttgcaa gatcattttt acataatgaa ataatttctt ttatattttt
     1561 ttcttctgtc tcacttatat ctaatttttc tttcaggcat tcaaaaattt tttgaagtcg
     1621 ttcaactctt tcatatattg aaccccattt tgggtgaaag acaatttttt ttaaatcatc
     1681 taaatatagt ttcaatggct tttgtgtatc cattttaatg aaaaagtttg catcttcaag
     1741 tttattgatt attacacttt cataatttgt ttttatttct tctttgtcaa ctccatcaag
     1801 aactattata aatttattat ataaactact gtcagaagta aaaagaggaa tacatttcaa
     1861 tttttttata acgacttctt ttactctatc aggaataccc ttatattttt cagaaatttc
     1921 tcctattttt aagattggat attcaactaa atttgatata ttctttacca aattttcatc
     1981 atatctacat tgaaatggca atgaattttt tattttttct tcaataaatt tttctcttac
     2041 attctggtca aaaatcacaa aatttttaag catcttatca ggatattctt ttatgtgttt
     2101 aatttttatt tttttaggag atagaactct atgaccagaa gtaaaatttg aacttttaat
     2161 atttccatat tttattggaa ttgttttatt atcaaataaa gataatatag accttatggg
     2221 tcttataaat tttatgtttt cttcattcca gttcattgac cttgggattt caattttttt
     2281 aagagaggtt aagaaaatat caggaagaag tttatatgtt ttattccctt tttctttttt
     2341 tatcgctacc agaacctttt tattattttt ttcttttatt tttaattgat taacttttac
     2401 attttggctc tctgcaaatt ttattgctgg taaagtatat tttccatttt tatcaattcc
     2461 aatatctgct gggggacctg aaatttctgc aatatagtcg ttttgaaaag gtgagacatt
     2521 ttttccatat aaaataattc ttctatttgt ataaaaaaga gaaatttcct caaaattaat
     2581 tcttttttct ttaatttctc ttgtaaaaac agggataaaa ttttctttaa aatattctcc
     2641 aatccattca ggtaagtcct cacatccaat ttcaattata aaataactca tttttctcct
     2701 gtggttaatg ttttacaatt atattaaatt ttcttccctc tttcaatttt ttaataataa
     2761 gttaaaatat tcttaaatag gataatataa tttgactttt ttgaagaata tcttagattt
     2821 tataatttaa aatttagagg ttttaaaata aaagtgtcac atttcatttt gtcgacaatt
     2881 ttcaaaaggt atattttaat tataaaataa gagaattaaa ttcgaaaagt aattttgcaa
     2941 gaccaattta aagagaggaa gaaaaatagg acaaaatttt agttttgttt tttcagagag
     3001 tttgttagca gaagtggcag gtattcctca gattaaattg cattttgatg tagaagcaat
     3061 ttgtaatgca tatgagaaaa ttaaacctat tgctaaaaga ttggatgtag aagcacctgt
     3121 accaagaata gcaggatttt gctatcctca tatagcatct ttgggagcaa aaattgtttt
     3181 tcctgaagat ggtgagccaa aaccatttcc aattataaaa agtccagaag aaattgataa
     3241 tttaacagaa cccaaagatt atttaaatgc acctataatt caagaaagat taagagtttg
     3301 ttatgaaata aaaaagcgtt atccaaaaag ccctaatttt attgggcatc tttttgaggg
     3361 accaataacc actgctgtgt taattatggg ttcaggtttt ttaactcttc catatgatga
     3421 ccctgaaaaa gcacataaac ttttaaattt cagtgttaaa agtgcgttaa attatgcaaa
     3481 tacactttgt aattattttg gagaaccaat acaaccaggt tctaaaggtc ttcctgatga
     3541 ttttgcagga atgttcccac caaaaatttt caaagaattt gttgctccat actgggaaaa
     3601 aatttatcaa ggaaataaag caacagaaag gtatcttcat agtgaattgt taaggttaga
     3661 acacttgcct tttcttaaag aactaaatat agaaaaattt gacccaagtg ctgaccaata
     3721 tcttaatcca gagttattat ctaaatattg tccatgtaag tttacttctc gcatattacc
     3781 atgggaaatt agggacttat ctgaagatga attagttgaa ttatataaag aaagagcgaa
     3841 atttaaacca tcttgtataa gtttttacct tggttgctta aaagacgaac ccaaaattaa
     3901 atgtcttcta aaaatagcaa gagaattaaa agaaaagtaa aaataggaga gaaaaaaatg
     3961 gataaaataa agtttgacga cttattatat atgagaaaag gtgataaaaa tttaactatg
     4021 ggacagagtc catctaaaat tgaaaaaatt attactgttg aagagtggga agtaaaagca
     4081 aaagccctaa gagaaatttt cttacagatg ttaggagaaa tacctcaaat ttcttgccct
     4141 ctatcctcaa aaataattga ggaaaataat tgtggagatt atattaaacg taaaattgaa
     4201 tattttgtag agcctgatga aaaaatttct gcttatgttt taattccaaa aaatttaaaa
     4261 accaaagtgc ctgctgtttt atgtattcat ccaacaacac ccttgggaaa agaacaaaca
     4321 ataggcaatg acaatactga aaagggacag gatagagcat atgcacttca tttagttaag
     4381 cgtgggtata taactatgtc ttatgacctt ctgactgcag gggaacgttg ttataaagga
     4441 tatgcacctt ttgatacagc gccattttat aaaaaatatc caaaatggtc agtaagagga
     4501 aaagatttat gggatgtaag tagagcaata gattttcttc aatcaatgga cgaagttgat
     4561 tcagaaagaa ttgggtctat tgggcattct cagggtggcg gaattactat tcattcaatg
     4621 gtattagata aaagaattaa agttggtatt tccagttgtg gaatgtggcc agaaaggtta
     4681 tctaaaaatc ctttcaactg ggcccggact ggatggtggg taggtattcc ttttttaaga
     4741 ccatattgtt atacaggtaa acattttccc ttagatatgc atgaatatct ggggcttatt
     4801 gctccgagag caataatgaa tatttctgca ttaaatgatt gtatgtacac aatagaagaa
     4861 gaaaatatca ccaggggtgc ttttaataac ctgagtgaga atgtatcaaa agttttttcg
     4921 ctatataaaa cagagaataa ctttaaaaat attctccaca tgaacgggca tagtttttta
     4981 ttagaacaaa gagaaattgc ctatgctttt ttagataaaa gattaagagg taataatatt
     5041 tagtgaatta tatattcagg aagtgcttgg aatatttacg aaaaatctat aaatttatgg
     5101 atagttaatt ccctatttgt aagtggaaat aaatattacc aaaggagaaa tatggagaaa
     5161 atagcacttt taaatgcaaa aattattgat ggcaaaaatt ctatcccgat ttcttcttgt
     5221 tcagttctct taaacaattc ttttattgaa gatataattg taaacaaaaa aatttctatt
     5281 ggagaaaatt atagaaaaat agatgtaaaa aataatactc tattgccact atttattgat
     5341 ggacatatgc atataagtgg tgagccagga agattagacc actttggaca tataaaagca
     5401 aatttaggtg cggtaggtaa attacaggaa tgtctcttat ggggaactgg tgttgtagca
     5461 catgcagcag gttctataga aaatgtaata cttcgtgatt taataaattc tggctctatt
     5521 ttagggtgtt ctgaaatgtt agtgggaggg gctattacac caacctgtgg gcatgtgaga
     5581 ggtaaaagtg cagatggccc ctgggaaata agaaaagcag taagagagat gataggagca
     5641 aatgttgatt ttataaaaac aactgcatct ggtggatttc aatgggaaca tgagaaatta
     5701 actcatactg attatactta tgaagaacta aaagtccttg ttgagcaaac tcattcaaga
     5761 gagaaaaaag tccatgttca tgctcatgct gaccctggca tttctaatgc tatttctgct
     5821 ggatgtgatg ttattttaca tggtgtctta ataaatgata tttgtttgga gaaaattgta
     5881 gaaaaaggtc tctggtatat gccaacatta tatataacaa gcgaaaaagt ttttgaaaac
     5941 cctaatttgc caatttatat gagagaaagg atgaaagaag taaatccatt tcatcgtgag
     6001 gcagtaggaa aagcattaaa gataggagta aaaataataa caggaacaga tggtggacct
     6061 ggttctatta tgtatgaaat atatgaattg gttaaatgtg gtttttctcc aatggaagca
     6121 attattactg ctacttcaaa aacagcagag gcatttggaa tacttaatag aacaggaaca
     6181 atagaaaagg gtaaaaatgc agatttgatg gtaattgaag gggacccgtt aactgatatt
     6241 tctattcttt gtaaaaaaga atctattcta atgataacaa aaaaggggaa aataattttt
     6301 aaccaatttg aacaagtaaa aaaataaaaa atggaaaatt caaaaaattc tcttgtccgt
     6361 cttattgtaa aaggtgttga taaatgtaag tcaaattttg ataaaaaaac agggcgattt
     6421 cttgctgaaa atggtggatg ggcagttaca aaccaggatg taatatttcc attaagttat
     6481 ctttatgtaa ataagcatcc agaaaatcct tattataaaa atgatgaaat tttgaatttg
     6541 tgtttttgtg gaggagatgc tttaaggaat gcccaagatg ctgatgggaa agttgaattt
     6601 gtcaaaattg atggttcaaa atggggaaag acctatatgt gctggtctat gtttcactgg
     6661 cttgagacat ttcgtctttt gaaaaatttt cttccatctg aacgcgctaa tagttggcag
     6721 gaaggtcttt atattgcttt tgaaggtatt tttaaagaac ttcaaaaatc aaaagtacat
     6781 aatatcccta catggaatgc aatggctatt tttaaagcag gtttgacttt ccaaaatgaa
     6841 aaatggcaaa aatatgcaaa ggaatttatt aaaaaagtag ttaaatcaat gaatccagat
     6901 ggctattggc aagaaggaca aggtcccaca acttcttata atcttgtata tgttcattcc
     6961 ttaggacttt attattgtgc atcaaaagat gaatttgtta ttccatccct taaaacagca
     7021 actaattttc atattcattt tacttatcct gacggttctc tcgtagaaac aattgatggt
     7081 agagtgaagt ataattctaa tccatcttgt atgggccttc ctggttttct tacaacagaa
     7141 aaaggcaaaa gatttagtgc atttttggta aaaaatcttt gtttgaaaaa cccaaaaggt
     7201 ctttgttcgc catatttaac ttctgcttta gaaatatggc ctgatgagat aaaaccgaaa
     7261 gataaaatta ttcaggaatt aacagaatat tcaagggtat ataatgggaa tgctattgta
     7321 agaaaacata aaaattttta tacctgtctt tcaggttatg tagctcctgc aaaaattcag
     7381 tcagagttat caaataatag atggatacag gataggtcaa attttgtttc aatctggtat
     7441 aagttatgtg gacttattat aggaggaggt aattcaaaag aacaacctac ctgggcaaat
     7501 ttttctctat ggaaaggaac atttcggcta tggtgtcctg atgcaagtca agtattttca
     7561 aaggaaaatg aagataaagt aatattggat tttgcaggta caaaatgttc aatatcggtt
     7621 aggttttcag gagaaaaaca ggtaaaaata atgtttgaag ttattaaatt ttcaggggca
     7681 gataaaattc aatcatccct tcttttgtgt cttgaaaaag ggacaagagt taaatgtgga
     7741 aaatataaat ttattgttga ccctatttat tcagaagtta tttttgttaa aaagagtcaa
     7801 actatccaga caaaacatta tcttataaaa tcgttaaaga aacatacatt tttttattgg
     7861 ccagaatatc cttttaatcc ttatgcaata aatggtgctt ctcctcctga atctgctcgt
     7921 ggaaagttaa tatttgaact cacttccaaa tcacctaaaa atgaaatttt aatacgagta
     7981 aaataatttt taaataaaaa ttgaagaaac aaaagaagaa gcaaaaaatg gataaattaa
     8041 aatcttaccc tttagataaa aaagtaaagg aactttttga acgacaaaga caattttcta
     8101 aaaaatggga atctacaaga attacaaagg agatttatat tgaattaagc gaatcaattg
     8161 taagagttgc tgtttcctgg caggataaag gaggtagaat aatagaccct tataagaaag
     8221 aagaaacatc tactgctaca gcccgttttg taggtgcatt aggatttttg attaggacgg
     8281 gacattgttt ggatttaatt gatatttgta taaaaagtat gaatagggca tgtgaagacc
     8341 tatattattc gtttgagaga ccacatatca gtggaccaga attttatgtt aaagaatgta
     8401 tgcagggata tttggcatta aaggataaag tagataaaaa attggttaaa aaatgggaaa
     8461 agtatcttgg tgaatatgac ccagaaaaaa attatgtagc agttctttct aaaaataagc
     8521 cagaagatat tcataatttt tgcacatttg gtttagcagg ggagagtttt aagaaagcat
     8581 ataaaatagc agataattct gatttcatag aaaggcattt agagacacag atacaatttt
     8641 tcactgaata tgggatgtat agagacccaa atgacccaat gacttatgat tggacagcga
     8701 ggatgaattt atcattattg gaatattttg gatataaagg gaaatattct aaagatttga
     8761 atgagatttt aagaagagga ggattaacaa cgcttttgtt tttatctaca actggtgaat
     8821 cgccttttgg aggcagaagt aatcaatatc atttcaatga agcgacaata tctttaattt
     8881 gtgagtatga ggcaaacaga tataaaagac aaggggaact acaaattgca ggtgctttca
     8941 aacgcactgc tagactaact gccttatcaa ttaaaagatg gttaggatta tcccctttta
     9001 gacatattaa aaatagtttc cctcctgaaa tccaacatgg atggcaacgc ggttatggtt
     9061 tttttagtgc ttattcttta cttattgcca gtcaatttgg ttttgcatat cttttagcag
     9121 atgactcaat tgaggaaaga caaactcttt atgaaattgg tggatatatt ttgaacttac
     9181 cggatgcttt tcataaagta tttgctacat gttgtggtta tcatatagaa atagacacaa
     9241 aggcagataa caattatgat gcaactggtt taggaagaat tcataaaatt tcaattccaa
     9301 ctgaacttgg gctatcaaca cctattgctt caaaaccaaa ttattctgtg tcagttgaac
     9361 catcattaag aaatgtatct attggtcctg gctgggagga taaggtagga aataaatatt
     9421 atctttctga tttaagtgat aaaatagaag atgtccaagt aaaaattatt aaggaagaaa
     9481 cagaagaagt taaatttgaa gttatatata aagggaaatt tgaaaataga ataaaagagg
     9541 catatacaat aaataaagaa gggatagaaa tagaggatga aataagtggt agtgtaaaag
     9601 atattttagt gcaaattcct ttattacaga cagatgggga aaatatttct gatattaaga
     9661 taggaaataa aaaatttgaa gtaaaatatc taaattgtat ttataaagtt gaatgtttag
     9721 attctggtgt agttgatact tttgttgagc cattttctgc acctaataga aatggaattt
     9781 ataaagttgg ttgttttaag acaaaaggaa attttattaa atacaaactt tcattagaaa
     9841 agtgtaaaaa ataaaaggag atttttaatt atgaataaaa aaatattgga aaatttaaga
     9901 aagggagtat ttattcctgc tcatccttta gcacttacaa aagaaagaaa attagatgaa
     9961 aaaaggcaaa agggattaac taaatattat attgaatcag gtgttggggg tattgcagtt
    10021 ggagttcaca caactcaatt tgaaattcac aatcccaaag taggacttta caaacctgtt
    10081 ctccagttgg caatagaaac aattaaagaa tataaacttg aagatgaaat aattaaagtt
    10141 gctggaatat gtgggaagac agaacaagca acaaaagaag gtgaaatagc aagggatatg
    10201 gggtatgatg caggtctttt aaatttagga acttttacag atgaaaaaga tgatgatatt
    10261 ttgattgaac atacaaaaga agtatctcgt attattcctg tatttggttt ttatcttcaa
    10321 ccagcaactg gtggaagaga attaagtttt tcattttggt gtaaattttt ccaaatagat
    10381 aatgttattg cagtaaaagt tgcccctttt gatagatata gaacaataga tgtaataaga
    10441 gcaattgtag aaacaggaag agaaaaagat attgctgttt atactggaaa tgatgataat
    10501 attattattg atttaattac tcctttcaaa tttaataata aaattataag gattgttgga
    10561 ggtttattgg gacattgggc attttggaca aaaaagagtg ttgatatatt taatgagata
    10621 aaagaaataa ttaaagaaaa tagtcctatc ccacaagaga ttttaacact ttccgcagca
    10681 ataacagatg taaatagtgc tgtttttgat gccaataaca aattttctgg gtgtattcct
    10741 ggtataaatg aaattttgag aaggtccgga cttcttgaag gaaattggtg tattaaccag
    10801 gatattacac tttctcctgg tcagaaagaa gaaattgata gaatttttag tgagtattca
    10861 tatttaaggg atgatgaatt tgtaaaaaaa aatctaaata aatggataaa atagaggaat
    10921 taaaaaaatt atatgaagat agaaatttaa gaagagaaat tgaaaaaagg ataaaagaaa
    10981 ttataggtat agatttatct gcgaaatatg gtaatttcta cctaaaaaat cctcttattg
    11041 ttgcgcctgg tcaacttaca agaacacaaa ctcagataag gcaaatacga gaggcgaact
    11101 ttggtggttg tgttttaaaa tcagttgttg gggaagataa agaaggtaat tgcagtatgg
    11161 aactttacag aactccacct acttatttaa aaagtattta tgaaaattat gatattgata
    11221 agaaatttcc tataatacat tgggatggta ggggagatac aagaaattta gatgagtatt
    11281 tatactttgc agaagatgtt tttaaatatg aaaaagataa tgaatttcta atcatatctt
    11341 caataattgc tcattttcca ttacctgatg aagaaataaa aaaagatgaa tggatatata
    11401 caacagagaa attatataat gtgggtgcaa ggatatttga aattgatttt tgcccccagt
    11461 tggagaaaga agaagaaaag attgaaaaag agaatatatt gagatggtat aaaaatattc
    11521 cttctattat aaaatcagta aaaaaagata tatctgtttt tccaaaaatt atgaaccttg
    11581 agtttgggat tgatttccag attgaaatgg taaaagcggc agttgaagga aaagcagatg
    11641 gagttgttat tgcaaacagg atttttaaaa aagaatttgg atgtgctcat ggtggaaaag
    11701 aattgaagga aaggaatata caacagataa aggaagtaaa aaaattattt ccagatgttc
    11761 atatttctgc aactggtgga atttatactg gtaaggacat tcttgattat attcaagttg
    11821 gggcagaaaa tgtgcaactt ttaagttttc ttatgggcaa gagttcaata aattttggaa
    11881 ttgaagggac aaaatttgaa aaagtttttt attttctttt atttgactct gaaactggtt
    11941 tactccctct cctaaaaatc aatgtttttt aaattataat ttattgtaaa ataatacaat
    12001 atgttaacct aaaaagatat tttttaatca cttttctgtt agtggaaaaa attagatgag
    12061 gggaaaagaa ataattaact aaaattttgg cagaaccaaa aatataacaa ggaggagaaa
    12121 aatggaggag aaaataaaag taggtgttat tggactaagg atgggattat cacatattga
    12181 aggttataga agaaatcctt atacagaggt ttatgggata tgtgatactg atgagaaact
    12241 attagaaaag acaaagcagg agtataatat taaaatcgca gttaatgact ataaagaact
    12301 tataaatata ccagaaattt ctattatttc tattgcttct cctgattttt ttcattgtca
    12361 gcaggcaatt tatgctttaa aaaatgggaa aaatgttttg tgtgaaaaac caatgacacc
    12421 aacaattgag gaggcaaaag aaattgttaa agtagtgaaa gaaacaggca aaaagtttat
    12481 ggtaggacag gtttgtagat atgctccagg ttttgttctt gcgaaaaaga tggtagaaaa
    12541 tggagatata ggggaattgt tttttgtaga aagtgaatat gctcacgatt attcaaaaac
    12601 gacaggaatt ggtaattgga gaaaagataa aaggagagaa ccatttcttg gtggtggttg
    12661 tcatgcagta gaccttttaa gatggattgc aggagaccct attgaagtta gtgcatattc
    12721 aaatcataag tgtttggttg actggcctgt tgatgattgt actattgcta tatataaatt
    12781 tccagatggg gttattggaa aggtttttgt ttccattggc tgtattagac cgtatacaat
    12841 gagaagtgtt ttttatggaa aagaagggac aattatatgt gataatacaa gtccacaaat
    12901 ttatttatgc tcaaagaaat attataggga gaaatcaaat tttaattttg cttcttttcc
    12961 tgttaatctt gcttctcaca atgtaagtgc agagatagat gaatttgtag attgtattat
    13021 aaataataag gaagtaccaa caaatgaaat agaaggtgca aaaacagtga tatcttgttt
    13081 aaaagcagtt gagagtgcaa aaaaggggaa acccttaaaa atagggggcg aattatgaaa
    13141 gcaggatttt ttcaattaga tattacacct tttataggaa tggaaagacc aagtggatat
    13201 agaaaggcat atgcagattt tattcatgac ccaacaaaag taagaagttg tattatagaa
    13261 gatgggaatg aagaagttgc tatcgttgta tcagatacat tagtttcacc accaagagaa
    13321 aatgttattg aaataaaaga agaaattgaa aagaaagttg gaataaaaaa agagaatatc
    13381 ttaattggtg caactcatac gcattcagca ggccctttat ttttcccatt taattatcag
    13441 gatgctgacc cccttacaaa aaaactcatt tctgaatatt caactatttc tgacccattt
    13501 tactatgaaa tttttaaaag gaaagttgtt gattctttaa tatgtgctga aaaaaagaaa
    13561 gaagatgtta tatgtaatgt cggttatgga tatgaagata aagtaagttt taacagaaga
    13621 tttaaaatga agaataatag agtttataca catcctggta aaaacaatcc tgatataata
    13681 gagccagcag gcccaataga ccattctgtt ggtgttttaa ccttctggaa taaagataaa
    13741 agtccaatag ggacttttat aaattttgcc tgccatttga cgacaggtcc aggaggtatt
    13801 tctgctgatt ggttatttta tactgaaaaa ataataaaaa aaatatttga agatatggat
    13861 ataatattat tacagggagc aagtggagat ataacacagg taaataattt atcagataga
    13921 gttgattttg gtgaaaagat atcacatttc gttgggacaa gagttgggct tgaggcattg
    13981 aaaattattt tgacagaaga aaaagggtct ctttctccat tgaaaattaa aaataaaata
    14041 attaatgtaa aaagaagaaa acaatcagag gaaaaattaa aaatggcaag agaaattgta
    14101 gaaaatggat taaagaatga aaatataaga caaacaactg aatggacatt tgcaaaagaa
    14161 agaataattc ttgattattt aacaaagaaa caaccagaag ttgaaataga aattcaggca
    14221 attcaaattg gtcctgttat aataatttca agtcctggag aaattttttc ttcaactgga
    14281 cttaaagtaa aaaaggcatc acattttcca ataacttttt ttgttgaact ttcgaatgga
    14341 tttcttggat atataccgga taggaaagca tttcttccga cgggcggtgg atttgagacg
    14401 gttttaacaa gtgaatcaaa tttagaggtt gatgcagaag aaaaaattat agaagagagt
    14461 ttaaaactaa tgaaggaatt taaaccaggg gaattaccag aatatgataa aataacatca
    14521 aaaccacagg tatggagtta tgggatatta ggtcctgata ttgattaaaa tggaaattat
    14581 tgatatacat gttcatccaa gacaggataa agaattaaaa gaggtagaga atttaataaa
    14641 ttttgctaag aaattaaata ttacaagaat aaattttctt ggagatgttc tttattatgg
    14701 atatcatcca aataatgagc aaataaaaaa aataaatgac ctgacaataa aacttgtaaa
    14761 aaaatatcct tgtttttttt caggtttttg ttttgtaaat cctgaacatg ataaaaaatt
    14821 tattaagcaa gaaattgaaa ggtgcatagt taaagggaat ttaaaaggaa taaaacttga
    14881 agcatcctgt aatgcatctg ataaaagggt ctttaaagtt gcagaaattg cagagaaatt
    14941 aaatgtgcca atattacatc atgcatggga tacaaccaat ataaatagta ggtggggtcc
    15001 agaagtatgt cagtcagacc cagaagatat tgcaatttta gcaaaaaaat ttccaaaagt
    15061 taaaataata atggctcatt taattgcttg tggagtaaga ggaattttag aaattaaaaa
    15121 ttttgataat gtttgggcag aaatatcagg aggacaacca gttacaggga taatggaata
    15181 tgcagtgaaa aagatggggg cagagaggat attatttggt tcagattgtc ctggtaggga
    15241 tttttctgct caactttcaa aagtatatgg agcaaaaata acagcaagag aaaaagaact
    15301 aatacttggt ataaatgcaa aaaatttatt agggctaaaa tgataattga cataaacata
    15361 aattggggaa actggccatt tgggaattta tattttaatt catttagaaa ttatattaat
    15421 aaaattaaaa ataaaggaat agaagaggga tttatttcac caattgatgg tattttttct
    15481 cctgaccctg atattctaaa tgaaaaagtt ataaaaaaat taaataagca aaacaacttt
    15541 aaatgtctac ctattataaa tcccaaaatt aaaaattggc aagagatatt agaaaagtat
    15601 aataaagaat ataaaattaa aattataaaa attgttccat cattccatag atattcaatt
    15661 attgaaaaag atgtctataa attttttgaa aaactttgtc aattagatat gagtcctgta
    15721 attcaaaaaa gatttgaaga tgaaaggtca aaaagccctg ttttaaatct taaggatata
    15781 gaaataaaac aaattgaaaa aatttcaaag gactttccag aattaaaaat aattatctta
    15841 tgtctttatt ggcatgaggt aattaaactt tctgaaaagt gtaaaaactt atattttgat
    15901 atttctttta ttgaatgtct tgatacactt ggagcacttt taaaatatat ttcagaagat
    15961 agggttatct ttggttcaaa tgttcctttt ttatatcctg aatcatctat attaaaactt
    16021 caatattcaa ctattacaaa agaacaattt gataaaatat catttaaaaa tttatcatct
    16081 tttcttgcaa aataaaacaa attatagtaa aaataaacat aaaaaccttt aacaaaaaat
    16141 ggaggaagga atgaaagtaa aaatatcttt tattggatgt ggtggaatag caggagcgca
    16201 tatgtctgca ctttcaaaaa ttgaagatgt tgaatttgtt gctttttgtg atgttgtaga
    16261 agaaaaggca aaacaaaagg cacaaattta tggaggtaag gtctttacag attataaaaa
    16321 aatgcttgat gaggaaaaag ttgatgcttg ttatatatgt attccgcctt ttgctcatga
    16381 tggacaggaa gaattatgta ttgaaaaagg gataccattt tttgttgaaa aaccagtcca
    16441 tttagttctt gaaaaatgta aagaaatagc gaaaaaagtt gaaaagaaga atttaattac
    16501 aggcgttggg tatgttttaa gaaattatga tgttgttaat aaagcaaaag aagtaataaa
    16561 aaatgaaaaa ataggtctgg ttagaggaaa atattttgga caggtaccag gtgctgaagg
    16621 aagttggtta cacaaaaagg aattatcagg aggacaattg attgaacagg caactcatac
    16681 agttgatatg atgagatatt taattgggga tgtagaagaa gtttttgctt ataagtttga
    16741 aggaataaat aacaaaattt ataaggacta taatgttgaa gatgcatcta ttgtagcaat
    16801 gaaatttaaa aatggagtaa ttggaaattt aagttgtaca tggttatgga gtggatggta
    16861 ttctggagtt gaaatagttg gtaaaggagt aatgcttact tatgagggga attcattaac
    16921 aatagataag ggtaataaaa aagaaatata tatttcacaa gaagatccaa tgttagtaga
    16981 agataaaaat tttatagaag gtgttaagac aggaaatagt aaaattatta aatcaaatta
    17041 tcaggatgca gttaaaacac ttgaagttac actaaaaagt catgaatcaa tagaaaaagg
    17101 agtaccggta aaaatttaat aatgaggtaa ttctctgcct gtaagggtag gactctttat
    17161 ttaaagccgt aagaagcatt atgcggatag ggaataaatt acttggtaca atttgttttt
    17221 gttctggtat tttcttcact tatgttctgt ataattttaa caagacaggg aacttaaaga
    17281 attttggtaa atacttactc aacacattaa ttcccttgcc cgtaggtcca cagaatttct
    17341 ctgggctacg cctgaggggg tggatttctc tatatctcct cttattaaac cccgtctgct
    17401 caacttatct ctaccttgcc tgcccgtctg agcattagcg aaggagggta gggctggttc
    17461 gtatgctata ttttaaactg cctgtgtaga acaatccccc tcccccgttg tgctatctgg
    17521 gagacgataa tgcaccaact ctgctttgct attcctacct tccttaatcg gtttatcaaa
    17581 gggggaactt atggtaaggg atgggagaag gagaagtggg aaaggatatg ataggtttta
    17641 atataattta ggaagaaaaa gagaaaaatc cgcaggatcc ctacggacga gccggatttg
    17701 acatctttaa aatatgtttt ataatttttg tagttaaaat tttatggatg gttgcttctt
    17761 gtttttacag ggaaaaatag acattacccc gtggatctta aacataaaca agttgaataa
    17821 atatttatac atgcttttaa aaatggagga gggagaatgg ggaaattagc aataaatggg
    17881 ggaaagaaag ttgttgataa ag
//
LOCUS       DAOOVW010000034        16962 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_2300_length_16962_cov_6.629503, whole genome shotgun sequence.
ACCESSION   DAOOVW010000034 DAOOVW010000000
VERSION     DAOOVW010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16962
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2300_length_16962_cov_6.629503"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            652..1248
                     /gene="nrdG"
                     /locus_tag="PLW95_05715"
     CDS             652..1248
                     /gene="nrdG"
                     /locus_tag="PLW95_05715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOV22160.1"
                     /translation="MRIGKYLISTGVNGPGKRFVIWFQGCSFHCKSCFNPEFWNKNGG
                     KVMNVKEMLSIIDKYSGIEGVTFTGGEPFLQSKELLSLSKLIRCKNLTIVCFTGYQYE
                     DILKGKIPYGKELLNYIDILIDGRFIKEEKTFLIWRGSRNQKVYFLSERYKYLKEFVE
                     KEGISEAEIKVGKKGISITGIFDINFWEELKKVLKEND"
     gene            1241..4513
                     /locus_tag="PLW95_05720"
     CDS             1241..4513
                     /locus_tag="PLW95_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22161.1"
                     /translation="MIRCPYCLQSDEPKSTKNNNTEVYTCTKCRSLLPRDFVEENYPK
                     MTVGIIGFTGHGKTVYLTSLFYLLKFIRRSSLWENFNWQTLDENTHKIVFEHVSLFEK
                     EHKLPKSTPANFPLPAIIEFFDMPLIGNCFFTFYDTAGAVYEEINSITNMGKFVAYAD
                     VVFFILSIPDCKRRKGRSWQDEMERFLDIYVNAVYNRLGISLEKFQHLIVVLTKADLF
                     IDGEEEPELKEFLLQGSYNWWGLKKTRNGEFRKKINEIAKFSEFIEDWLEEKGGGGFI
                     NLARRRFKSVKYTLISAIGSAPKGKKLVSLSPEDPKRVIDPLMLIIQKIFEGEGEEER
                     VVSDTVKELTTSEQVTQPIPDNEILQSIYTKVPKRKSYFRKGDFHTVFYPTKFLNRND
                     ISEIEKRIHFPGDIFDEKITLFFQEIQKKWCLVILKIKNLPNEKDSLNRGGLFLCHAF
                     IFPENIWKRTGDLLSLVKFTEGFSFTKIKDIFNSPFFDRRNGNSNNIKILPEKLGEIS
                     NTLPKLSNFNSDFVWKIIILMNRIAREKNNNIPVILKGNPENITDFLNKIFAYIPDDF
                     KVLIGFDSCFDNGNLASYPLKIVGYKTTLPTNTSSIIIDLEKSILKVPDGIEELCNSR
                     SCFEIWLNSLREKIKYKEEIEKAYKLSEIIEEGKDYSDKEILTGEDQKIFVSINKERI
                     IERFYSYLKGLIWEEVVKVVIKEISIDKMFEIFFDRGKIKDICGNIVGVIIREKIPII
                     PGRSYIKEELLSEGNNIIRAMWKLAKGEKLSNKDIECLKEDERREFLRYLVLTEEEKE
                     DWLLDILGTNRDIVEQLLKDNKIKDTLNKERIIERFYSYLKGLIWEEVVKVVIKEISI
                     DKMFEIFFDRGKIKDICGNIVGVIIREKIPIIPGRSYIKEELLSEGNNIIRAMWKLAK
                     GEKLSNKDIECLKEDERREFLRYLVLTEEEKEDWLLDILRTNRDVLEQLLKDNKIKDT
                     LKPIIEEILLQNKHLFKKTFDILEEAIFSINNEYFVLEYQHLQSHEIFVNVFISLLKS
                     KKKDEKTVEDIYKWVKKNSPDRIEETSEIIKEYQNIDPYDFKFIGKLKRFLRKIWSKK
                     GM"
     gene            4722..5750
                     /locus_tag="PLW95_05725"
     CDS             4722..5750
                     /locus_tag="PLW95_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22162.1"
                     /translation="MSGLKYSNVYIEEQIRKKQEIISQIENTKAEIFILKDKGKNIIG
                     EFSQGIKEEFKMEIEKFKSWQKNKIPNFSFYMYIHELQAVKKEMEEFKCIGMKQFKAL
                     LEIKEKAPKKIQAVKKEMEEFKGIGMKQFKSFLEIKEKTLKKIQALKNKMEELSIILK
                     SIEILSTKWHLEEYAEIEKAIDLLSHSLEEEKVSLVESEIEKLNLKINKMKETLNGYE
                     NLNKQRQYVLESLRKVCKQIGWEEIKEPYFEKKDDPRSRIIYEINTYSAGIMKFYLTL
                     EKIVVNSPLTNENNYCIKEFNNLSEKLKNFGVNTKFKRDTAENEDPMLYGKNRISTID
                     EESLENNR"
     gene            5771..6901
                     /locus_tag="PLW95_05730"
     CDS             5771..6901
                     /locus_tag="PLW95_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22163.1"
                     /translation="MSELENLLIDLVDAGWYTRENAYKKLNSKYPDWYFTSYIKDLLP
                     VYIKALESEDDSIRYSAVNVLKLIKDKLAIEGLIISLVDSNALVRKISADCLSAIDIS
                     KIHPGWHKGEVVKNYISKIISALFSENEDVRNKAQKLINKIYPNGENWIENKEIVNNL
                     FLYCTKLLSSGNKKKILESKNLINKILDKLDFKKYSEKGILDFISALEIDNEEVREIV
                     INILVEKNNEFLSSYPILFCRKCYLKRPAKIKIYNNIDREFIVCQHCRESNYLEKGVN
                     RVIGVIGADFENFKREGDVVYINLWDNKTKRAVNADIDELEIRKKDEIDYAAVVDSIC
                     LELKNDISRDKGYLKTIPVVLKTDISKEVKDLLKKQFKEIRK"
     gene            6912..8756
                     /locus_tag="PLW95_05735"
     CDS             6912..8756
                     /locus_tag="PLW95_05735"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOV22164.1"
                     /translation="MSVWMENLKQLLTQKEILILYGNVRDTTYIKKNGDLVKGLTELI
                     WEIGKEFGYDEERFWGVFYNNENKSQSPMWAIERIDIFNEEGGKSEVEEINVQDKEVV
                     LLTKWLQKYIMDINKKRIFVIYYIDKLTPYNPRGPYPKDKEILITLIQKIIENISSNN
                     RLIMVSLQDTMIPFDYYTNSPKVAVMEIPKPGKEERKIYFSKHLIDFNQEHLDFLANI
                     TDGLYVRDIDNILRDVLSQKKDISNSILRKIVNRYRIGTEEDPWENLPLSGSPKGLID
                     SAENWFKEKVIAQDYAVEEVVKAIKKARAGVVGLASGQLTKPKAVFFFAGPTGVGKTF
                     LAKKLAEYLFDSESAFVRMDMSEYKEEHTVSKLIGSPPGYVGYEEGGQLTKSVKSKPF
                     SVILFDEIEKAHPKIMDIFLQILDDGRLTDSRGQIVFFTECVIIFTSNIGARTTNNRG
                     NPISERENLEKILSNKEISEEQKQEMVREHFVEAVREFFMYEISRPELLNRIGSHIIP
                     FNYISIPESQRKIIKSKLEDIKNNFQDKFFYAGHILEFKEDVSNYFFNKHRENIEKFG
                     GRGLVNIIEDEIGPLLAEQLLFAEKTGIIKVKFTFFVSENGELKCRRE"
     gene            8741..9826
                     /locus_tag="PLW95_05740"
     CDS             8741..9826
                     /locus_tag="PLW95_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22165.1"
                     /translation="MQKRVIQKADISFTANLSNSIVLVIHPSKEFKDKIFYIGKEIVS
                     RLPGRIEGIYFLGSNKKYTISIPRDFEENFPRWFNDNENRVNLISPIFEEFEKNSYYS
                     TIAVICSSIPYDLDDWNANKIIKRTLFINVSDNHSNKNYYTEINLKGGISEIINALND
                     PAINIQIRGQGFIPIFYEIHSKGKIETPVYKKGEFIINIILEEEFIDVHLKAFFLKNP
                     PYLYIKREKGVEEKIQGKIGKFIVPEIIWQELPKELIPVFEAFFSKSEYICYQCGKKH
                     LYTTLVCPEGSVVLKNIPLGKVILFNKEYMFYPLSDFAFVFEKSGKIITNDGKLYKLH
                     NEKLDFIKNIELCEEVEKNVFALYHKI"
     gene            9798..10406
                     /locus_tag="PLW95_05745"
     CDS             9798..10406
                     /locus_tag="PLW95_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22166.1"
                     /translation="MCLLFTIKFDKFGFPLIKHNNWNFYISLLPVSKFQFEKFMIKNG
                     PLGYFYTDEWYKKILSLNKRNNWKKINENPWGIFITGLEYKEIVPFLKYLGKDFRLPT
                     VEEWICLYKVTKELKEEKESLFKQCKKNCSFPALYWIKNDFYPLTEEGLVEIVKDEKG
                     QIKHIGKPFNKWLPNLWQANTVRDIFEETKYLAGFRVAITDK"
     gene            complement(10464..10811)
                     /locus_tag="PLW95_05750"
     CDS             complement(10464..10811)
                     /locus_tag="PLW95_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22167.1"
                     /translation="MAEITQKNLTLKEIEKGTIDTLKNIEQLEKVADSYISLKEEEIF
                     PYFFRERTVSLFEIMKKRLEEEKEKNLQLLEQLKHMSPQPNGCGCMTILIILAVTLFI
                     FIILFPKFTFLMQ"
     gene            complement(10930..11745)
                     /locus_tag="PLW95_05755"
     CDS             complement(10930..11745)
                     /locus_tag="PLW95_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22168.1"
                     /translation="MEQLIVDLDSDKFRFLRWGKNIKEYPSLKFYRPFRIPDLGNIYI
                     QENDTVVIGKVSFKEIYYGFFKINKIESEKDIPTNENEIFCFALLKNNKKENYEVLLN
                     FLETTYGPGFLVERGGIISKRKLESEGTDVEVYQWEGKQTGIEIEYIEETENIFVRYY
                     SSIILNKLLTDVEEELHSVIENTEENENNSVINGYTPLKCPYCGGVAKYETTVRQGSP
                     ADNINAAILGGLLTRSFFGGLLAGNMMADRNNQRVRIYRCERCKNTIEIPEKG"
     gene            12732..12935
                     /locus_tag="PLW95_05760"
     CDS             12732..12935
                     /locus_tag="PLW95_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HOV22169.1"
                     /translation="MAEIKFFEVIDCMTCKHIRKNGKGNFVCDAFPDGIPLKMIVESG
                     HRRKLPGQRGDVVYEPKKWLKEV"
     gene            12958..13101
                     /locus_tag="PLW95_05765"
     CDS             12958..13101
                     /locus_tag="PLW95_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22170.1"
                     /translation="MEVIIRVCKTCGEETEDLTCPNCEVATVSLSKAMNFTIISKNED
                     EYG"
     gene            13274..14188
                     /locus_tag="PLW95_05770"
     CDS             13274..14188
                     /locus_tag="PLW95_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22171.1"
                     /translation="MKITLKTNHPLFQRLKDNPPQWWQNLKSDPEIYIDIRKENYLNV
                     YHNGGSIMKLGGTKGYKAQIHTEYIPLNTTAKYLPFEFQEGNISLKELQTIDIRNFER
                     EALERIKKRIRKFYPNGSEKGIQGQYVINNNKKRRGEGFFIDTEMAYGNNRKDMKRID
                     MVWVNMKTREIAFVELKTMGDDRLYATERESNETIDKQLKKYYEFAKGNRNDLIEYYN
                     RVYQVKKELGLLPRFAKDDSLKNYRLIERPILLVGDCTQQWIDKNAEDINNWLKDIAF
                     GCLYQGKTNYNFKIPYEKSQNCFPLAEE"
     gene            14194..15492
                     /locus_tag="PLW95_05775"
     CDS             14194..15492
                     /locus_tag="PLW95_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009146542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOV22172.1"
                     /translation="MKFKIHRGTREIGGSCVEIWTESRRIVLDLGMPLVNPDRTRFDS
                     RILRNASVQELIDKGILPDIKGLYEEPGNTALILSHAHQDHFGLIKYVHENCKIYLGK
                     ASEVLIGLSSIFLNEKWDISNPVNFESGKMFSIGDIEIIPYLMDHSAFDSYAFLIKDG
                     GTSLFYSGDFRRHGGKTKAFDWFAHNVEKNVDYLLLEGTTIGRIDVELKTEDDLEAEL
                     VKIFKEGKGINLIYTSGQNIDRLVSIYRACKRTGKTLAIDFYIAAVLTELSKFGKIPY
                     PSKNFPEIRVFFPYRLSRMISDRGNKDILYRFKEFKITKAQISGQFDKTVMIVRPSMK
                     TDLEHIKNLKNGIFIYSVWSGYKKEDATQEFIKFLTSRGMIQREIHTSGHADRNTLKR
                     MVEVLNPKNLVPIHTFEGDKYEKIFPGTKVLRIDDNEVIS"
     gene            15686..15952
                     /locus_tag="PLW95_05780"
     CDS             15686..15952
                     /locus_tag="PLW95_05780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOV22173.1"
                     /translation="METVIIGERGQITIPKELRKKYKFNPKTPVIIEDRDGEIVIKPA
                     VVVSMDKIKSLLKEYDNEFIQEMVKSLSLSSDEEKKILKKWTKK"
     gene            complement(16523..16702)
                     /locus_tag="PLW95_05785"
     CDS             complement(16523..16702)
                     /locus_tag="PLW95_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22174.1"
                     /translation="MAVDSIILSFSGNLLRISNSIITSSTLINFQKSLFDTSIFCGIF
                     FSMLYFTSSSTKFEK"
BASE COUNT         6605 a         2047 c         3086 g         5224 t
ORIGIN      
        1 gaataactgg cagataattt atcttacaat ggatgaccac attaaagatt tatttaaaga
       61 ggcatcccat ctttctcctg ttgattttaa tttatattcc ttataaattt ttaaccctgt
      121 gtttataaat gtaaaaatca ctggcccgct tttcctccag accaggtctg gggaaatggc
      181 agaaaaaaag ggtagggggt tttgcgggag atggagatgc ttgatagaat tcccctcttt
      241 gacaaagagg gtatctctta cccacaagtt ccccctttgt aaaccgacta aagaagcaaa
      301 gggtagcaaa gcagagttgg tgcttgtttc caaaatactt ccccctttgt aaagggggat
      361 ggagggggat ttataagcaa aacggggatg gaggggaatt tcaaccttct tcccacaggg
      421 gagaaggcaa taagatattt ttgacgataa tggattacct ttcaaacgac aattgaattt
      481 gacaatttta aaagtgtatt ttaaaatcaa attgcgatat taaaatgaaa actataaaat
      541 ggtggtttta tggaaaatgt agaatataaa aaatatctta tcagtgtttt tctgcagatt
      601 atctcatatc tttgcattat agttattatc tatgtcaaaa aaacatatta aatgcgaata
      661 ggaaaatatt taatttcaac tggagttaat ggtccaggaa aaagatttgt aatttggttt
      721 caaggatgtt ctttccattg taaaagctgc tttaatcctg aattttggaa taaaaatggt
      781 gggaaggtaa tgaatgttaa agaaatgtta tctattatag ataaatattc tggtattgaa
      841 ggagttacat ttacaggtgg agagcctttt cttcaaagta aagagctttt atctctttca
      901 aaactaataa gatgtaaaaa tttaactatt gtttgtttta ctggatacca atatgaagat
      961 attttaaaag gaaaaattcc ttatggtaaa gaattactga attatataga tatacttatt
     1021 gatggtagat ttattaaaga agaaaagaca tttttgatat ggaggggaag tcgtaatcag
     1081 aaggtatatt ttctttcaga aagatataaa tatctaaaag aatttgtaga aaaggaagga
     1141 ataagtgaag cagaaataaa agtaggcaaa aaaggaattt ctataactgg aatatttgat
     1201 ataaactttt gggaagagtt aaaaaaagta ctaaaagaaa atgattagat gcccttattg
     1261 tttacaaagt gatgaaccaa aatcaacgaa gaataataat acagaagtat atacttgtac
     1321 aaaatgtaga tctctattac caagggattt tgttgaagag aattatccta aaatgacagt
     1381 tggtattatt ggatttactg ggcatggtaa aacagtttac ttaacatcac ttttctactt
     1441 attaaaattt ataagaagaa gttctttatg ggaaaatttt aattggcaga cgcttgatga
     1501 aaatactcat aagatagtat ttgaacatgt ttctttattt gagaaagagc ataaattacc
     1561 taaatctacc cctgcgaatt ttcctttacc agcaattatt gaattttttg atatgccatt
     1621 gataggtaat tgttttttca ctttttatga tactgctgga gcagtgtatg aagaaattaa
     1681 tagtataaca aatatgggaa agtttgttgc atatgcagat gtagttttct ttattttaag
     1741 tatacctgat tgcaaaagaa gaaagggaag gagttggcag gatgagatgg aaaggttttt
     1801 ggatatatat gtaaatgcag tgtataaccg acttggcatt tcactggaaa aatttcaaca
     1861 tcttattgtt gtattaacaa aagcagacct ttttattgat ggagaggaag aaccagaact
     1921 taaagaattt ttattacaag gttcatataa ttggtggggg ttaaaaaaaa caagaaatgg
     1981 agagtttaga aaaaaaataa atgaaatagc caagttttca gaatttatag aagattggct
     2041 tgaagagaaa ggaggtggtg gatttataaa tttagcaaga aggagattta aaagtgttaa
     2101 atatacatta atatctgcaa taggttctgc tccaaaaggt aagaaattgg tatcgctttc
     2161 accagaggat ccgaagagag taatagaccc attgatgtta ataatacaaa aaatttttga
     2221 aggagaagga gaagaggagc gagtggtaag tgatacagtg aaggagttaa ctacttctga
     2281 gcaagtaact caaccaattc ctgataatga aattttacaa tccatttata caaaagttcc
     2341 aaaaaggaaa tcttacttta gaaaaggtga ttttcatact gtattttatc ctaccaaatt
     2401 tttaaatagg aacgatatat cagaaataga aaaaaggata cattttccag gtgatatatt
     2461 tgatgaaaaa ataacattat tttttcaaga aatacaaaaa aagtggtgtc ttgtaattct
     2521 taaaatcaag aatttaccta atgaaaaaga ttctttaaat aggggggggt tatttctctg
     2581 tcatgctttt atatttcctg aaaatatatg gaaaagaaca ggagatttat tgagtttagt
     2641 taaatttaca gagggatttt cttttacaaa aataaaagat attttcaatt caccattttt
     2701 tgatagaaga aatgggaata gtaataacat aaaaatttta ccagagaaat taggagaaat
     2761 ttctaataca cttccgaaat tatctaattt taactctgat tttgtctgga aaattattat
     2821 attgatgaat agaatagcca gagaaaaaaa taacaatata cctgtaattt tgaaaggaaa
     2881 tccagaaaac ataactgatt ttttgaataa aatttttgcc tatattcctg atgactttaa
     2941 agttttaatt gggtttgact catgttttga caatggcaat cttgcttctt atcctttaaa
     3001 aatagtagga tacaaaacaa ctcttccaac aaataccagt tctattatta ttgatcttga
     3061 aaaatccata ttaaaagttc ctgatggaat tgaagagtta tgtaattctc gaagttgttt
     3121 tgaaatatgg ttaaattctc ttcgtgaaaa aattaaatat aaggaagaaa ttgagaaggc
     3181 atataaactt tcagagatta ttgaagaagg taaagattat tcagataaag aaattttaac
     3241 aggtgaagac caaaagatat ttgtttccat aaataaagaa agaataatag agaggtttta
     3301 tagttattta aaaggtttaa tctgggaaga agtagtaaaa gtagttataa aggagatttc
     3361 tattgataag atgtttgaga ttttttttga tagagggaaa attaaggata tttgtggaaa
     3421 tatagtagga gtaattataa gagagaaaat accaattatt ccagggagaa gttatataaa
     3481 agaggagtta ttaagtgaag ggaataatat aatcagggcg atgtggaaac ttgcaaaagg
     3541 agagaaatta agtaataaag atatagagtg tttgaaggaa gatgaaagga gggaattttt
     3601 aaggtattta gttttaacag aggaggagaa agaagattgg ctgttagata ttttagggac
     3661 aaacagggat attgtagaac aactccttaa agataataaa ataaaagata cattaaataa
     3721 agaaagaata atagagaggt tttatagtta tttaaaaggt ttaatctggg aagaagtagt
     3781 aaaagtagtt ataaaggaga tttctattga taagatgttt gagatttttt ttgatagagg
     3841 gaaaattaag gatatttgtg gaaatatagt aggagtaatt ataagagaga aaataccaat
     3901 tattccaggg agaagttata taaaagagga gttattaagt gaagggaata atataatcag
     3961 ggcgatgtgg aaacttgcaa aaggagagaa attaagtaat aaagatatag agtgtttgaa
     4021 ggaagatgaa aggagggaat ttttaaggta tttggtttta acagaggagg agaaagaaga
     4081 ttggttgtta gatattttaa ggacaaacag ggatgtttta gaacaactcc ttaaagataa
     4141 taaaataaaa gatacattaa aaccaattat tgaagaaata ctacttcaaa ataaacacct
     4201 tttcaaaaaa acttttgata ttttagaaga ggcaattttt tcaataaata atgaatattt
     4261 tgttttagaa tatcaacatt tacaaagtca tgaaatattt gtaaatgttt ttatatctct
     4321 tttaaaatcg aaaaaaaagg atgagaaaac ggttgaggat atttacaaat gggtgaaaaa
     4381 aaattctcct gatagaatag aagaaacgag tgaaattatc aaagaatatc aaaacattga
     4441 cccatatgat tttaagttta taggtaagtt gaaaagattt ttgagaaaga tatggagtaa
     4501 aaagggaatg tgataaaatt atatagaaca aaaacaaaaa aaaatatata atttagtaaa
     4561 tacccagtca attgtattga ctgaaggtag caagaataaa agtaaaaaaa caaaaagatt
     4621 taaaggtttt ttctctctac ttagatatga ccgaggctaa tatttatata gtttataaaa
     4681 aaccttttga aagagatttc caatgaggag tcaaggggaa aatgagtgga ttaaaatatt
     4741 caaatgttta tatagaagaa caaattagga aaaaacagga aataatatcc caaatagaga
     4801 atacaaaagc agaaatattt attttgaaag ataaaggcaa aaatattata ggtgaatttt
     4861 ctcaaggaat aaaagaagaa tttaaaatgg aaatagaaaa atttaagagt tggcagaaaa
     4921 ataaaatacc aaatttttct ttttatatgt atattcatga acttcaggca gtaaaaaaag
     4981 aaatggagga atttaaatgt attgggatga aacaatttaa agcactttta gaaattaaag
     5041 aaaaagcacc taaaaaaatt caggcagtaa aaaaagaaat ggaggaattt aaaggtattg
     5101 ggatgaaaca atttaaatca tttttagaaa ttaaagaaaa aacacttaaa aaaattcagg
     5161 cacttaaaaa taaaatggaa gaattatcta taatacttaa aagtattgaa atattatcta
     5221 caaaatggca tcttgaagaa tatgcagaaa ttgaaaaagc aattgactta ctttcccatt
     5281 cattagaaga agaaaaggtc tcgttggttg aaagtgaaat agagaaattg aatttaaaaa
     5341 taaataaaat gaaagaaaca ttaaatggat atgaaaattt aaataaacaa agacaatatg
     5401 ttttagaatc tttaaggaaa gtttgcaaac aaattggatg ggaagaaatt aaagaaccat
     5461 attttgaaaa gaaagacgac ccaagaagtc ggattatcta tgaaatcaac acttattcag
     5521 caggaattat gaagttttat cttactcttg aaaaaatagt ggttaattca ccattaacaa
     5581 atgaaaataa ttattgtata aaggaattta ataatctatc tgaaaaactt aaaaattttg
     5641 gtgtaaatac aaaatttaaa cgtgatacag cagaaaatga ggaccctatg ttatatggaa
     5701 aaaacagaat atccacaata gatgaagagt cattagaaaa taatagatga aagggattta
     5761 ataaataaaa atgagcgaat tagaaaattt gctgattgat ttagtagatg caggatggta
     5821 taccagagaa aatgcttata aaaaattaaa ttctaaatat ccagattggt atttcacctc
     5881 atatattaag gacctcctgc ctgtatatat caaagcattg gaaagcgaag atgatagtat
     5941 aagatattca gcagtaaatg tattaaaatt aataaaagat aagttagcaa tagaaggact
     6001 aataatttca ctggtagatt ctaatgcatt agtaagaaaa atatctgcgg attgtttaag
     6061 tgcaatagat attagtaaaa tacatcctgg gtggcataaa ggagaagtag taaagaacta
     6121 tatatcaaag ataatttctg cattattttc agaaaatgaa gatgtaagaa acaaagcaca
     6181 gaaattaata aataaaatat atccgaatgg agaaaattgg atagaaaaca aagaaatagt
     6241 aaataattta tttttatatt gtaccaagtt gttatcaagt gggaataaga aaaaaatcct
     6301 tgaatctaaa aacctaataa acaaaatttt agacaaatta gattttaaga agtatagtga
     6361 aaaaggaatt ttggatttta ttagtgcatt agaaatagat aatgaagaag ttagggaaat
     6421 agttataaat atacttgtag agaaaaataa tgaattttta agtagttatc caattttatt
     6481 ttgccgtaaa tgttatttaa aaaggcctgc aaaaataaaa atatataaca atatagatag
     6541 agaatttatt gtatgtcaac attgtagaga atctaattat ttagaaaaag gggtaaatag
     6601 agtaattgga gttataggtg cagactttga aaattttaaa agagaaggag atgtagttta
     6661 tataaattta tgggataata aaaccaaaag agcagtaaat gctgatatag atgaacttga
     6721 aatacgcaag aaagatgaaa ttgattatgc tgcagtagta gattcaatat gtttagaatt
     6781 aaaaaatgac atttcaaggg ataaaggata tctgaaaaca ataccagttg ttttaaaaac
     6841 tgatatttcc aaagaggtta aagacctttt aaagaaacaa ttcaaagaaa taagaaaata
     6901 aaggagagat tatgtctgtc tggatggaaa atttaaaaca actattaaca caaaaagaaa
     6961 tccttattct atatggaaat gttagagata ctacatatat caagaaaaat ggtgacttgg
     7021 tgaaaggact tacagaactt atttgggaaa ttgggaaaga atttggatat gatgaagaaa
     7081 gattctgggg tgtattttat aacaatgaaa ataaaagtca atctcctatg tgggcgatag
     7141 aaagaattga tatttttaat gaagagggtg gaaaatcaga ggtagaagaa ataaatgttc
     7201 aagataaaga agttgttttg ttaacgaaat ggctccaaaa atatataatg gatataaata
     7261 aaaaaagaat ttttgttatt tattatatag ataaactcac tccttataat ccaaggggtc
     7321 catatccaaa agataaagaa attcttataa cacttattca aaaaataata gaaaatattt
     7381 cttcaaataa tagattgata atggtatctt tgcaagatac tatgattcca tttgattact
     7441 atactaattc cccaaaagtt gctgtaatgg aaattccaaa accgggtaaa gaagaaagaa
     7501 aaatctattt ttccaaacat ttaattgatt ttaatcaaga acatcttgat tttcttgcaa
     7561 atataacaga tgggttatat gtaagagata tagacaatat tctaagagat gttttatctc
     7621 aaaaaaaaga tatttcaaat tctattttaa gaaaaattgt aaatagatat cggattggga
     7681 cagaagaaga cccttgggaa aatttaccat tatctggttc tccaaaagga ttaattgatt
     7741 ctgctgaaaa ttggtttaaa gaaaaagtta tagcacagga ttatgcagta gaagaagttg
     7801 taaaagcaat aaagaaagca agggcaggag ttgtaggact tgcatcagga caattaacaa
     7861 agccaaaagc agtttttttc tttgcaggtc ctacaggagt tggaaaaact tttcttgcaa
     7921 aaaaattagc cgaatatcta tttgattctg aaagtgcctt tgttagaatg gatatgagtg
     7981 aatataaaga agaacatact gtttctaaac tcattggttc accccctgga tatgttggat
     8041 atgaagaagg aggacaactt acaaaatcgg ttaaaagtaa acccttttca gttatattat
     8101 ttgatgaaat agaaaaagca caccctaaga taatggatat ttttcttcaa attcttgatg
     8161 atggacggct tacggatagt agaggacaga ttgttttttt tacagagtgt gtaattattt
     8221 ttacctcaaa tataggtgcc agaacaacaa ataatagagg aaatccaata tcagagcgag
     8281 aaaacttaga aaaaatttta agtaataaag aaattagcga ggaacaaaaa caggaaatgg
     8341 taagagaaca ttttgttgaa gcggtaagag aattttttat gtatgaaata tcaagaccag
     8401 aacttcttaa caggataggt tctcatataa ttccttttaa ttatataagt ataccagaat
     8461 cccagaggaa aattataaaa agtaaattgg aagatataaa aaataatttt caagataaat
     8521 ttttctatgc aggacatata ttagagttta aagaagatgt tagtaattat ttctttaata
     8581 aacataggga aaacatagaa aaatttggag gtagagggct tgttaatatc attgaggatg
     8641 aaataggccc tttactcgct gaacaattac tttttgcaga aaaaactggg attatcaaag
     8701 taaaatttac tttcttcgtt agtgaaaatg gagaattgaa atgcagaaga gagtaattca
     8761 aaaagcagat atttctttta ctgcaaatct ttctaactcc atagtgcttg ttatacaccc
     8821 atccaaagaa tttaaggaca aaattttcta tataggaaaa gaaattgtca gtagattacc
     8881 aggaaggatt gaaggaattt attttcttgg ttctaacaaa aaatatacaa tttctattcc
     8941 gagagatttt gaggaaaatt ttccaaggtg gtttaatgat aacgaaaata gagttaacct
     9001 tatatccccg atttttgaag aatttgagaa aaactcttat tatagcacaa tagctgtaat
     9061 ttgttcttct attccctatg atttagatga ttggaatgca aataaaataa taaaaagaac
     9121 actgtttata aatgtttctg ataaccacag taataaaaat tattatacag agattaactt
     9181 aaaaggagga atatcagaaa taataaatgc attgaacgac ccggcaataa atatacaaat
     9241 tagaggacag ggatttatac caatttttta tgaaatacat tcaaaaggaa aaatagaaac
     9301 tcctgtgtat aaaaaaggag aatttattat aaatattatt ctagaagaag aatttataga
     9361 tgtacattta aaagcgtttt ttttgaagaa ccctccatat ctttatataa aaagagaaaa
     9421 aggtgtggaa gaaaaaatcc aagggaaaat tggaaaattc atagttccag aaataatatg
     9481 gcaagaactt ccgaaagaat taatacctgt gtttgaagca tttttttcaa aatcagaata
     9541 tatttgttat caatgtggga aaaaacattt atatactaca ttagtttgtc ctgaaggaag
     9601 tgtagttcta aaaaacatac ctttagggaa ggtgatttta tttaataaag aatatatgtt
     9661 ctaccctctt tctgattttg cttttgtttt tgaaaaatct ggaaaaataa taacaaatga
     9721 tggtaaactt tataaattac ataacgagaa attggacttt ataaaaaaca ttgaactttg
     9781 cgaagaggta gaaaaaaatg tgtttgctct ttaccataaa atttgataaa tttggtttcc
     9841 ctttaattaa acataacaac tggaattttt atatttcact tttgcctgta tctaaatttc
     9901 aatttgaaaa atttatgata aaaaacggac cattaggata tttttataca gatgaatggt
     9961 ataaaaaaat tctctcattg aataaaagaa ataattggaa aaaaataaat gaaaatccat
    10021 ggggtatctt tattacagga ttagaatata aagaaattgt tccattttta aaatatttag
    10081 gtaaagattt taggttacct acggtagaag agtggatttg tctttacaaa gtgactaaag
    10141 aattaaaaga agaaaaagaa agtttattca aacaatgtaa gaaaaattgc agttttcccg
    10201 ctctttactg gattaaaaat gatttttatc cattaactga agagggatta gttgaaatag
    10261 taaaagatga aaaaggtcaa ataaagcata ttggaaagcc atttaataaa tggctgccta
    10321 atctctggca agcaaataca gttagagata tttttgaaga aacaaaatac ttagcagggt
    10381 ttagagttgc tattacagat aaatgaggat aacaaaattt atttttgtta ataattgtat
    10441 aattatgttg tttttcttat taactattgc attaaaaaag tgaattttgg gaatagaatt
    10501 ataaaaataa ataaagtaac agcaagaatt attaatattg tcatacatcc acatccgtta
    10561 ggttgcggag acatatgttt taattgttct aataattgta aatttttttc tttttcttct
    10621 tccaatcttt tcttcattat ttcaaacaaa gaaactgttc tttctctaaa aaaatatggg
    10681 aaaatttctt cttctttcaa ggaaatataa ctatctgcta ctttttcaag ttgttctata
    10741 ttctttaaag tatctatagt acctttttct atttctttaa gggttagatt tttttgagtt
    10801 atttctgcca tattttatat tttaatatac tttaatgtta attatttcta taccttttta
    10861 aaaactaact actcacttta aaaaacttac ataattataa tttaccagaa aactgtcttt
    10921 tctcctctat tatccttttt cgggaatctc tatagtattt ttacatcttt cacatctata
    10981 aattctaact ctctggttat tcctatctgc catcatattt cccgctaata accccccaaa
    11041 aaaactcctt gttagtaatc ccccaagaat agcagcattt atattatctg ctggagaccc
    11101 ctgtctaaca gttgtttcat attttgccac tcctccacaa tatgggcatt ttaaaggcgt
    11161 atatccattt attacagaat tattttcatt ttcttcagta ttttcaatca ctgaatggag
    11221 ttcctcttca acatccgtta ataatttatt cagtataata gacgaataat atcgcacaaa
    11281 tatattttct gtttcttcta tatattcaat ctcaatccca gtttgcttcc cttcccattg
    11341 ataaacctct acatctgttc cttcactttc taattttctc ttgctaatta ttccacctct
    11401 ttctactaaa aatccaggtc cataagttgt ctctaaaaaa ttaagtagca cctcataatt
    11461 ttctttttta ttatttttta aaagagcaaa acagaatatc tcattttcat tagttggaat
    11521 atctttttca gattctattt tgtttatttt gaaaaaacca taatagattt ctttgaagga
    11581 aactttccct ataactactg tatcattttc ctgtatatat atgtttccca aatcaggaat
    11641 acgaaaggga cgataaaatt ttaaggaagg atactctttt atattttttc cccatcttaa
    11701 aaatctaaat ttatcactat caaggtctac aattagttgt tccattatag aaatttatta
    11761 actaatttga cactttgttt ttattttcta tttatatatt acaatttatt aattttttgt
    11821 caagttaact tcgccatttc attttgggca attatgaaaa cacttgtaaa taaaggggtt
    11881 gaagagattt tgatttatta ctacatttca ctacatattt gcagaaaccc gtatgaaata
    11941 aggatttttc atttttgagt ggcgaagttg acttgtcaat ttgataacac tattacagaa
    12001 aaacttttat tacaatatac tttaaaagta aatatttact caagtctccg tttatattct
    12061 taggcacaaa gtaaagggat atcggaataa gaggttaatt gttctacaca gtccacaggc
    12121 tcgcccgtag ggtctacgcc cgagggtagg gatgagggtg gttttataag gggcgatttt
    12181 gtttttagat atgtctttgt gttgcagtat ttgggtgtga gtgaatatta taaaatatct
    12241 cctgtaaatc accgcaaacc ccaaaaaggc aaaaaagggt agggggtttt gcgagagatg
    12301 gaaatgcttg atagacaagg gattttgcaa aaataaataa agttgttaat ataatttcag
    12361 gtagtaaaag aagggatttg cggaaaaggt agttaaaacc cttgatatgt ttgctctttg
    12421 acaacccaca gtaagaatca ctgacccgct taatagggga ttacgacaaa ccatacaatc
    12481 cacttactac taatactact atatcgtaag aatcactgac ccgcttaata ggggattacg
    12541 acattgttca aaagtcgtta gtgatgtgag gaaattatca ccaatgggaa aggaattaag
    12601 tagtgttgta ggaattagta tatgtgagga gaggagacag tagcaacatt gataggtaga
    12661 tatttagatg gtagtttaga gagcaagtat gtaaaagtgc ttaagaagta tatattataa
    12721 aggagtaaga tatggcagaa ataaagtttt ttgaggtgat agattgtatg acctgcaagc
    12781 atataagaaa aaatggtaaa ggtaattttg tttgtgatgc ttttccagat ggaatacctt
    12841 taaaaatgat agtagagagc gggcatagaa gaaaattacc tggtcaacgg ggcgatgtgg
    12901 tatatgaacc gaaaaagtgg ttaaaagagg tataatatta taaagagaat gtaactattg
    12961 gaggtaataa taagagtatg taagacatgt ggtgaagaga cagaagattt aacttgtcct
    13021 aattgtgagg ttgctacggt atctttatca aaagcaatga attttacaat aatatcaaag
    13081 aatgaagatg agtatggtta gaatcactga cccgcctaat aggggattat ttaaccgcgt
    13141 aagtttaacc cccgcggtta gaagaggatt gacgaaggaa tataaatgac ttaaattgag
    13201 aagagtgcta atagtgaaaa acaagatgcc cggatgaagt ttgctatgtt gtgaccgcaa
    13261 aggagcaata gatatgaaaa tcacactgaa aactaaccat ccgctatttc aaagacttaa
    13321 ggataatcct ccccaatggt ggcaaaacct gaaatcagac ccggagatat acattgatat
    13381 aaggaaagaa aactacttaa atgtatatca taatggcggc tctatcatga aattgggagg
    13441 aacaaaagga tacaaagcgc aaatacacac ggaatacata ccgttaaaca caacagcgaa
    13501 gtatctgccg tttgagtttc aagaaggaaa tatctcatta aaggaactgc aaacaattga
    13561 tataaggaat tttgaaagag aggcgctgga aagaataaaa aagagaatcc ggaagtttta
    13621 tcccaatggt tcagaaaaag gcatacaggg ccagtatgta atcaataaca acaagaaaag
    13681 aagaggcgag gggtttttca ttgatacaga aatggcgtac gggaataatc ggaaagatat
    13741 gaagcgtatt gatatggtct gggtaaacat gaaaaccaga gaaatcgcat ttgtagagtt
    13801 gaaaacaatg ggcgatgaca ggttgtatgc aactgaaagg gaaagcaacg aaacaattga
    13861 taaacagctt aagaaatact atgaatttgc gaaaggcaac aggaacgatt taattgaata
    13921 ctataacaga gtgtatcagg ttaaaaaaga gttgggactg ttgccaagat ttgcaaaaga
    13981 tgattctctt aaaaattacc ggcttattga aagacccatt cttctagtag gcgattgcac
    14041 ccagcaatgg attgataaga atgcagagga tataaataac tggcttaaag atatagcttt
    14101 tggttgttta taccagggga agacaaacta taactttaaa ataccctatg agaaatccca
    14161 aaattgcttc ccactggctg aggaataagg tatatgaaat tcaaaattca cagagggacc
    14221 agggaaatcg ggggctcctg tgttgaaatc tggacagaat caaggcgtat agttcttgat
    14281 ttgggtatgc ccctggttaa tcctgacagg acacgctttg attcaagaat cctcaggaat
    14341 gcctcggtgc aagaactgat tgacaaaggc attctgccgg atattaaagg gttgtatgaa
    14401 gagccaggca atacggcatt gattctatct catgcccatc aggaccattt cggcttaata
    14461 aaatatgtcc atgaaaactg caaaatctac ctcggcaaag catctgaggt attaattggg
    14521 cttagcagta tctttttaaa cgagaaatgg gacatatcaa accccgtgaa tttcgaatcg
    14581 ggaaaaatgt tttctatcgg cgatattgaa ataatcccat acttaatgga tcactccgca
    14641 tttgactctt atgcgttttt aatcaaggac ggaggaacat cactgtttta tagcggcgat
    14701 ttcagaagac acggaggaaa aacaaaggca tttgattggt ttgctcacaa tgtagaaaaa
    14761 aatgtagatt atttgctgct tgaaggtaca actatagggc gaatcgatgt agagttaaaa
    14821 accgaagatg atttagaggc ggagttggta aagattttta aagaagggaa agggattaat
    14881 ctgatttaca cttccggcca gaacattgac cggctcgtct caatctatag ggcatgtaaa
    14941 agaacaggaa agactctcgc tattgatttc tatatcgctg ctgttctgac agaactgtct
    15001 aaattcggca aaattccata cccatcaaag aacttcccgg aaataagagt ttttttcccc
    15061 tatcggctct caagaatgat tagcgaccgg ggaaacaagg acatactgta ccggttcaag
    15121 gagtttaaaa tcacaaaagc acagattagc gggcagttcg ataaaacagt gatgattgta
    15181 cgcccgagca tgaaaacaga cttggaacat atcaaaaacc tgaaaaacgg aatcttcatt
    15241 tattccgttt ggagcggata caaaaaagaa gatgcgacac aggagtttat aaagtttcta
    15301 accagcaggg gaatgattca aagagaaatt catacaagtg ggcatgctga tagaaataca
    15361 ttgaaaagaa tggttgaggt tctgaatccc aaaaatcttg tcccaatcca tacttttgaa
    15421 ggggataaat acgaaaaaat attcccagga acaaaagtgc tgcggataga tgataacgag
    15481 gtaatttcct gaaggttgaa aaacctgcaa acccttgata cacttgctct ttgacaaccc
    15541 acggttagaa tcactgaccc gcttaatagg ggatttctct ccagaccagg tgtggaggag
    15601 taagaattca gaaaaaagat aactgctgga tggctgttga cttattttct gaaaatatgt
    15661 ataataaagg aaaaagagga ataatatgga aacagtaatt attggagaaa gaggacaaat
    15721 aacaatacct aaagagttaa gaaagaagta taagttcaat cctaaaacgc ctgttattat
    15781 agaagataga gatggggaaa tagtaattaa gccagcagta gttgtttcta tggataaaat
    15841 aaagtccctt ctaaaagagt atgataatga gtttattcaa gagatggtaa aatcattatc
    15901 tctttcttca gatgaagaaa aaaagatatt gaaaaagtgg acaaaaaaat aaaagttttt
    15961 tgtgatagta atatattttt ctctgcaatt tatactgatt ttaaaaattc gtaaatactt
    16021 actcaaccac attaatttaa aagcacaaaa gcccccctca ccctcgccct ctcccctgtg
    16081 ggtagagggt aataagagga aaaaagttct tctccccagc ggggagaaaa tagatgcagg
    16141 gatagggtgt ttttctcttt atagaaatgg aaagacagaa aagtagggaa gaaatttaat
    16201 atggttaata actcctgcgt ctaaaaaggg tatgggaaaa aggatttttc ccatatttat
    16261 aataatgtat ttcgtattgg gggtatcgtt gggaaagggg aagcacccct gtcccaagag
    16321 cgaagcgacg gatgaggggg tgattttcat atcagtacct ggtattgtct gcaggaacct
    16381 gtggaactgt ttgattgcaa gaccagttcc tgattactct gtgcctttgt gcctaagaat
    16441 ataaacggac ttgagtaaat atttacaaaa attcttatcc ttccttaatt ataaaacttg
    16501 ctgaaaataa cttttttgat atctatttct caaatttggt agaacttgaa gtaaaataca
    16561 acatagagaa aaaaatccca cagaaaatag atgtatcaaa caaacttttc tgaaaattta
    16621 taagagtaga agatgtaatt attgaattag aaattctcaa taaattacct gaaaaagata
    16681 gaataatact atcaactgcc atatattaca gaatggattt ttttattaca ggaaatgtca
    16741 aagatttttc ttatttactt aacaaaaaaa taggcgagac actggtttta tctccaaagg
    16801 aattttgtta taaagaaaat gaaaaataaa ataataaaaa ggtgtagatt tgtaattcaa
    16861 attaccgcaa gtcccaatac gcataagttt aactcctgag gtaaaagaac tgttgtgtca
    16921 aataaaaatc tactcgaaat actaattcgt tgagacatta ag
//
LOCUS       DAOOVW010000035        16308 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_2404_length_16308_cov_6.861256, whole genome shotgun sequence.
ACCESSION   DAOOVW010000035 DAOOVW010000000
VERSION     DAOOVW010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16308
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2404_length_16308_cov_6.861256"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1050)
                     /locus_tag="PLW95_05790"
     CDS             complement(<1..1050)
                     /locus_tag="PLW95_05790"
                     /inference="COORDINATES: protein motif:HMM:NF026221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22175.1"
                     /translation="MIIPEWAINARVLAVRYQEIDKLEKIANYYQSNVVILYLVPETS
                     NGWCPEGTYEQISNFISKVHKYGIKILTYFDSVCVEEKFYYGEHKDWVQRNPEGQPQH
                     YQPSHISQHRYCNCINSPWRNYLLEIVKKEAKIGSDGILFDNPGYYEFCGPSCFCKYC
                     QEKFEKEKGKSIFNVSRDEQKLWAHGQIGLFLKEAYEIFQKISSDKEIVITSNYSGPT
                     YQNMKLLSAGENVLFHETSGQRGKELLKILKEDREIGGKKPIWTIRTIGVKGKEGYSS
                     DNVSEYEILIAEAVACGANPVLWCAPTSMSPENPGFVLPPFYEIPEFSQMVSSYYSFI
                     KNYEKLFFNRDVKSFF"
     gene            complement(1061..2086)
                     /locus_tag="PLW95_05795"
     CDS             complement(1061..2086)
                     /locus_tag="PLW95_05795"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOV22176.1"
                     /translation="MDKELKIAFIEVGHYHAGMYLNFIKKTKHKIIAVSDKNKEIAQK
                     IAKEFTCHFYSDYHKLLKKEDIDFVFSFGRHCEMPEIIDSILERGIPLCTEKPITNNA
                     GIMKKLSEKANKKGLFTDVVLPLRFSPIILAIKEFSEKNNIGEIVHCYFRNMAGPIQR
                     YIDWRNKWLLDKKIALGGSFINEGPHYIDLFRYLTKEETKFVYAGMNNCIYGKSIEDN
                     SSVILETASGKRGVIEICYGYPTDRNWRDFAFVINTTNFLFTLIDIEKPRIFKLEIRC
                     RKNNKVEITNLLKYNQDVYNVFLEEMLKRYLNNQPGIIPFNYYYGIVKVLDAIYKSVM
                     YKKVIQI"
     gene            complement(2071..3030)
                     /locus_tag="PLW95_05800"
     CDS             complement(2071..3030)
                     /locus_tag="PLW95_05800"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOV22177.1"
                     /translation="MKAAIIGACGGNAGNHIRAYKKLGIKISAIVDVNKKGKKLAREL
                     GAEFYSDYKDINESITDIASVSMPPYLQPIVTSHLLKRGISVLCEKPISPTVKEGKKL
                     RKIISSTGKILMVGFCFRFSQKFIKLKELIESNVIGETLYINARYSAASNFKDNWRTD
                     IKKGGGILLVNSIHYADLAPWLIGQKIETVDAYGSSRFHLKEAEDNSILILKHKDVCV
                     TTVSGHYWIFKKSVVDFEVIGTKARATFEKNCIILDKNNGREEKVEYKENDMYYAEVK
                     HFIECVKNKTSPQCGLEESLQATNIIEKARLSMLKGKRIWIKS"
     gene            complement(3304..6789)
                     /locus_tag="PLW95_05805"
     CDS             complement(3304..6789)
                     /locus_tag="PLW95_05805"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HOV22178.1"
                     /translation="MKIRSFILFIFVLPFIVFSEISIKEENDFISFEKGKTVLIFNKK
                     SAYGTVSINLDGKKICEIVPGFTKLYTTKEKDKYEVVNYWLPTGVQNLEYYINRDREK
                     VELIIFRNFPDISVIEHYTFDEKESFINYVAEIKREKEGFSLESNIYFNLSDSKELVW
                     EQNKTTPELLGDYWEKSIEIKNSRWLIGYNAEEKNGLIIIRDYDFDPDAACHHFRIFR
                     GPTLGIKITQRDFLSRISLRIGVFENEIPLEVVKKINLTPCLPSQYSFVDEQRVYPMI
                     NEEEKIEIDGKIEEEIWKKLKKENNFYKFEWNELKSPYFADAQTEFYGFYDKENIYLA
                     VKCYEPEIKNLEAEEKTGSQNVWRDDCIELFIQPKGKDDYYQFIINPKGEKQDNKGIV
                     KKWKANGYIGNNFWSIEVVIPFSILGCSPVDLDVWKFNICRERRHISELSSWNETSGT
                     NGFHQPEKFGEIIFSSKIYPTKITLLKSKDYIGIESEIINMENILKEPLRILVSIPDD
                     ETIERIYQLNLKKGGREFISLFEKANVDKGYNINFDITPKIGIPLYSKTFKKTGYSGL
                     ISRLWPLSYDKILYIKENTIQQFAFIFANYTNSPQNFNLRLEVPKEIELLFLDRQYNF
                     SYGQFFPLIEEKAEEIDKEYGKYKKYELKFSKPIPVRTLPSQEEEKIYERLILPFSIG
                     KTNLKEFKIYFHIENNEKKIVEKENEYLVKTVAEFNAKMPKSIESGVFADWYFGPFQA
                     SVKEEKKKELVLESASSWEKSGFNILITSVNNKEIRDELNKKGFRIRTSLWWFWWDKE
                     YLEKHPESYAITFNGDKAKYEGIDTVCPTLMIEDEEAFLNTKKMVQKLVTEKGNDIWH
                     DTEGPNAWEICFCDRCIKSFRKYAGIDEKEELTPLLIKSKYKEEWREFASWQQAQIFL
                     KIKNSIKQANPDARFANYGGIKEPDYRCNWEILSKYNSIDIAGPSMYELSPLTLKYWE
                     KEMENFRKRVGNLKVTPWLDNEARQRNYKLLRTQALKSITIGCDGYHIYYDTLISVDG
                     RRLFDLGVVNTIISDFEDFFLNGKKIQGEIFIDENKDDYSIDGWEIGEEKVIFIFNHD
                     DKNKKEVEFSLPFEIEEKIVFYDYMNKKNIEEANKISLSLYDLGIKIIYIGPEEKLKE
                     RIKIWGNNLINFYKE"
     gene            complement(6812..10030)
                     /locus_tag="PLW95_05810"
     CDS             complement(6812..10030)
                     /locus_tag="PLW95_05810"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HOV22179.1"
                     /translation="MKQVLCLFMFLFCQVLFANGYTVHWFEAEDFKGNSNIVGSNSAS
                     GGAFVEGKTWYHFVMGIPFPQDGKTYNVWTRVQSDVPANWYLYENSEKKRAFGWFHTD
                     KPDEWTWVLIGKFSNSIGETFYSIYLQKPIDKNIPTATGRMDVVVITDIDNADVLENL
                     LKQRIEKKNEKVSESTKENEKEKFLLSSRRLSDVPYIKIPPIIDGVLNDEAWQKAAKL
                     GDFTLTNGKLATEQTIGYIAYDDNNLYIAAEMLESQIPFIRKVRTKRNDNVWQDDCLE
                     IFIDPGFTEKEALHFIVNPIGTRQDNLSVRIDRVTAIGEEGLDWKVATTIGKDKWFVE
                     ASIPFRLLDVNKPGEGEIWGFNLNREQRPKGELSHWNYVGGDFNQPDKFGLISFGQKP
                     VTLEAVTFGELFKDITFSLKAIDGDTKNLSIFLTIAKGEDVFIKSNKEISITGNKTKK
                     ITFPLSIEKPGFYNLSAVIKDSEKVLYELKFPIKTYSESLVSVAWPPEMRGGNLYIAL
                     NTAQHCFFLFANHFPDKRQITNPIFVVSVPSGVKILDPMGSLLIGPERYNNVTSWNIS
                     EESKNGQIYHKYTFNLEKNIPPSRIEKVRFFQNSLVLFFTIENGVFSAGEKIPAYFKL
                     EADGIEEEENMMYLVTLPNIEGKQPKNILIHNWLWTFNTCPSIWKPYLDTLQKAGFNS
                     ISVETDKSYYSDMAKEQYHMIIINNFWWFWWNNEYLKEHPEHTAITFNGVRDSSPLPR
                     ICPTILLENNGEVLRESIKGFSQSVTNGITDGYIWDLEGPGAWDVCFCKRCLDEFRKF
                     AKISQEEELTPRGIKEKYANEWILFATHQSSMLSEVIRNELKKNNPNATWGVYSGMPG
                     QMTMESYRCNWEEIANYIDIAYPTGYSNSPSFLDDRFVSGWKEFIKLMEGTLPRKHRV
                     KTVPTLTPGYERGAAMQPSADLIRMQILRSVASGADGVSFWWWGPMDGLYYQEIGRAT
                     ALIAKFEDFFLKGKNNNSIIKIEKNPYGKNVSWEVFTLGNKHLVILFNHSANETRKLS
                     FSFPGMDTEISGYFYPEGKKFNPLRETTVEIKPLEIAVLILQ"
     gene            complement(10077..10817)
                     /locus_tag="PLW95_05815"
     CDS             complement(10077..10817)
                     /locus_tag="PLW95_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007276711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOV22180.1"
                     /translation="MRKKTGFTLIELLVVIAIIAILASMLLPALSQARERARSAVCMN
                     NLKQIGTAMYMYLNDYNEYFPYGWPKRCLHRALAPYLGYKDAQYEYGGSRGPMRCPSQ
                     RAKYAGPSYGISVYFFRDYWLYPTTQDTFKPVKLSKVRHQERIVMVCDGKYNADPKGE
                     YNPSLDQIYTSTSGGYIEYDSTVWPPYATPSTWGTFSPRHHGMTNVLMVDGHCESLST
                     NDLWYKNATDNPSGHASTRLYYWYSGWF"
     gene            complement(11073..>11878)
                     /locus_tag="PLW95_05820"
     CDS             complement(11073..>11878)
                     /locus_tag="PLW95_05820"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOV22181.1"
                     /translation="FHNESEISQNTYEDILLRELRKEATKNGYTILLKNWQKEEIEYA
                     EVTKESSGIIVLNPKRNDNELINRLSKYSIPSVVIGANLERKDINYVASDNKGGMRQA
                     VEYLISLGHKNIFFIGGSIESHDTYERYEEFMNYCKKRKIQHNEVILESNFDWSKEVE
                     KILEENLKEKNLATAFITGGITLSLYLIDALKKADKKIPDDISLIGFDEFPICSHITP
                     PLTTIYQPIELLAKKGFEILEEEKKKKNKKRKQVILETKLIIRESVKRR"
     assembly_gap    11879..11978
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12350..13345
                     /locus_tag="PLW95_05825"
     CDS             12350..13345
                     /locus_tag="PLW95_05825"
                     /inference="COORDINATES: protein motif:HMM:NF017509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOV22182.1"
                     /translation="MKVEIIKKSELGRELNFIVEKEVVDETKRKILDEIKKDAEIDGF
                     RKGKAPDDLIEKKYSSAIQEQLLRKLITDSYINAIKENNFNPIVEPDVYDAKFDDNGN
                     LLFKIYIEERPDVKIEKYKEIPVKKVKPKEVTEKMVEDVLKEWEKTPEMKVSLIDIEK
                     RKAWKEKAREQLIAYSKARAEMEEEKQLWDELMKNIKFPVPEKLVNERAIRYTEEQLK
                     AMNLENKSKEEVEKIAKEIFQKVKPMAEEDVKKYFILEKISELENINVDEKDVEERIE
                     HISRVIGQPYEKIKEQIEKNGEMENLKEEIKIDKTFRFIKENANMIERIILPGEK"
     gene            13347..13967
                     /gene="clpP"
                     /locus_tag="PLW95_05830"
     CDS             13347..13967
                     /gene="clpP"
                     /locus_tag="PLW95_05830"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011994267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOV22183.1"
                     /translation="MEKKNEIKNQVIPYVIEQTERGERAYDIYSRLLKDRIIFIGTAI
                     TDNVANVVIAQFLFLQSEDSGKDIHLYLNTPGGAITAGLAIYDTMQFVSCDVDTYCIG
                     QAASMGAILLAGGTKGKRFLLPHSRVMIHQPWGGVEGTATEVNIHTREILRLKKIVNE
                     ILSLHTGQSIKKIERDTERDYFMSAEEAVKYGISDKIIQSEKVKKL"
     gene            13977..16307
                     /gene="lon"
                     /locus_tag="PLW95_05835"
     CDS             13977..16307
                     /gene="lon"
                     /locus_tag="PLW95_05835"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009333165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOV22184.1"
                     /translation="MAIIKKKIVKNNNPAIPLRDLVIFPNMIVPLLVGRTRSLNSIEE
                     ALNNDKYLIVVFQKSPLIEEPTFDDIHSTGVVVKIMQSIREPSGTMKILVEVIKRVKI
                     KNFISNKKYYIATVEEIREETQVKDKETEALMRLLLELVEKYIDLNPSVPKEIYSTIS
                     VIEEPSKLCDTIAGYLPLQVKDKLKVLETIPVKERLKVLIDIFNNEIGILEVQKQIQG
                     KVIKKIEETQKQYFLTEQLKEIEKELGQEGEGPEIKQLKDAITKAKMPKNVEDKALEE
                     LSRLSKMMPMSPEATVIRTYLEYLINLPWSVSTEDNTDISNAEKILNEDHYGLEKVKE
                     RILEYLAVKKKTDSLKGPILCFVGPPGVGKTSLGKSIARALGRKFVRISLGGIRDEAE
                     IRGHRRTYIGALPGRIIQSLRKVGVNNPVFLLDEIDKIGTDFRGDPSSALLEVLDPEQ
                     NHSFSDNYLEVEFDLSKILFITTANNPYTIPAPLLDRMEIISIDGYTIWEKKGIAKHF
                     LIPKQMKEHSLGDDEIKFSEASIIKIIKNYTKEAGVRNLEREIANVCRKVTKKIVQEN
                     KKRKYIITSKNLEKFIGIEKFTETEVEKEPKVGVATGMAWTEYGGEILFTEALTMKGK
                     GNLILTGQLGDVMKESAQAALSYVRGHAEEFGINPDFYKDIDIHIHVPEGAIPKDGPS
                     AGVTIVTSLVSVLSGKPVRGDVAMTGEITLRGKVLKIGGLKSKILAAHISGIKKIIIP
                     IENKKDLIDLPKKIRDELEIIPVETVKDVLEKALIQ"
BASE COUNT         5560 a         2706 c         2236 g         5706 t
ORIGIN      
        1 aaagaacgat ttaacatctc tgttaaaaaa taatttttca taatttttaa taaaactata
       61 ataagacgat accatctgtg aaaactctgg aatttcataa aatggtggta gaacaaatcc
      121 aggattttca ggactcatac ttgttggagc gcaccacaaa acaggatttg ctccacaggc
      181 gactgcctca gcaattaaaa tctcatattc tgagacattg tcagaagaat atccctcttt
      241 cccttttacc ccaattgtcc ttattgtcca aattggtttt ttcccaccaa tttctctgtc
      301 ttcttttagt atttttagta gttctttacc tctttgtcca cttgtctcat gaaataaaac
      361 attttctcca gcagaaagca atttcatatt ctggtatgtt ggtccagaat aatttgaagt
      421 aataacaatt tctttgtctg aagatatttt ttgaaatatt tcatatgcct cttttaaaaa
      481 taatccaatt tgaccatgtg cccataattt ttgttcatct cttgaaacat taaatatact
      541 tttacctttt tctttttcaa atttctcctg acaatattta caaaaacatg aggggccaca
      601 aaattcataa tatcctggat tatcaaataa aataccatca cttccaattt ttgcctcttt
      661 ttttacaatt tctaaaagat aatttctcca tggggaattt atacaattgc aatatctatg
      721 ttgagaaata tgagatggtt gataatgttg tggttgtcct tctggattcc tctgaaccca
      781 atctttatgt tctccataat aaaatttttc ttctacacaa acagaatcaa aatatgttag
      841 aatttttatt ccatacttat gaactttact aataaaattt gaaatttgtt cataagttcc
      901 ttctggacac catccatttg aagtttccgg gactaaatat agaataacaa catttgattg
      961 ataataattt gcaatttttt ctaatttatc tatttcctga taccttactg caagaactct
     1021 tgcatttatt gcccactctg gaattatcat tttattttac ttatatttga attaccttct
     1081 tatacattac tgatttataa attgcatcta agaccttcac tattccataa taataattaa
     1141 atgggataat acctggttga ttgtttaaat atcttttaag catttcttca agaaatacat
     1201 tgtaaacatc ctggttatac tttaataaat ttgttatttc tactttatta ttttttctac
     1261 aacgaatttc caacttgaat attcttggtt tttctatatc aataagagta aaaagaaaat
     1321 ttgtagtatt gattacaaag gcaaagtcac gccagtttct atcagttgga tatccataac
     1381 atatctcaat cactcctctt tttccactcg ctgtttccag aataacactt gaattatcct
     1441 caatactttt tccatagata caattattca tacccgcata gacaaatttt gtttcttctt
     1501 ttgtaagata acgaaaaagg tcaatataat gtggaccttc gtttataaat gaaccaccca
     1561 aggcaatttt cttatctaac aaccatttat ttctccaatc aatatatctc tgaataggtc
     1621 ctgccatatt acgaaagtaa cagtgaacta tctctccgat attatttttc tcactgaatt
     1681 cttttattgc aagaataata ggactaaatc ttaaaggaag aaccacatca gtaaaaagac
     1741 cttttttatt tgccttttca gacaactttt tcataattcc tgcattatta gttattggtt
     1801 tttctgtgca aagagggatt cctctttcaa gaatactatc tataatctct ggcatttcac
     1861 aatgtctacc aaatgaaaaa acaaaatcta tatcctcttt tttcagtaat ttatgatagt
     1921 cagaataaaa atggcaggta aattcttttg ctattttttg ggctatttct ttgtttttat
     1981 cagagacagc aataatttta tgttttgttt ttttgatgaa gtttaaatac atcccagcat
     2041 gataatgccc tacctcaata aaagcaattt ttaactcttt atccatattc tcttaccttt
     2101 caacatagat aatctcgctt tttcaataat atttgtcgcc tgtaaacttt cttccaatcc
     2161 acactgtgga gaagttttat tttttacaca ttcaataaaa tgttttactt ctgcataata
     2221 catatcattc tctttatatt ctaccttttc ttctcttcca ttatttttat caagaataat
     2281 gcaatttttt tcaaaagtcg cacgagcctt tgtacctatt acttcaaaat caaccacact
     2341 tttcttaaat atccagtaat gtccagaaac tgttgtaacg cagacatctt tatgtttcaa
     2401 aattaaaata ctattatctt ctgcctcttt caaatgaaaa cgagaactac cataagcatc
     2461 cacagtttct attttctgtc caataagcca gggagcaagg tcagcatagt gaatactatt
     2521 tacaagtaaa atcccgccac cttttttaat atcagtccgc caattatctt taaaattact
     2581 tgcggcactg tatcttgcat taatatataa tgtttctcct atcacattag attctattaa
     2641 ctctttaagt ttaataaatt tttgagaaaa acgaaaacaa aagcccacca tcagtatttt
     2701 cccagttgat gaaataattt tccttagttt tttaccttct tttaccgtag gagaaattgg
     2761 tttttcgcat agaacactaa tacccctttt taaaagatgg gaagtaacta ttggctgtaa
     2821 atagggtggc atactcacac tggcaatatc agtaatactt tcatttatat ctttataatc
     2881 agaataaaat tctgcaccta attctctggc taatttcttt cctttcttat ttacatcaac
     2941 aattgcactt attttaattc ctaatttctt atatgcacga atatgattac cagcattccc
     3001 tccacaagcg cctataattg ctgctttcat tatttttccc tcctatatga aaaatcaaaa
     3061 acattgcttt aacacgtatc atatttttaa gaactttcaa ctaatttaaa gtaattaaaa
     3121 tcctcaaaaa ccaattatat tttaatattt tataaataat agataaaata cttttgattg
     3181 aaaaacaaaa ttatttgaaa aatattccat taaaaagaaa ggtaaagaga acaaatttca
     3241 tattatttta tcctgcttta aatcttattt aaaaatttgg aaagatataa aaatatttta
     3301 tatttattct ttataaaagt taattaaatt atttccccag atttttatcc tttcttttaa
     3361 tttctcttcc ggacctatat aaataatttt tatgcctaaa tcataaagag aaagagatat
     3421 tttatttgct tcttcaatat tctttttatt catataatca taaaagacaa ttttttcttc
     3481 tatttcaaat ggtaaagaaa actcaacttc ttttttattc ttatcatcat ggttaaatat
     3541 aaaaattact ttttcttccc caatttccca gccatctata gaataatcat ctttgttctc
     3601 atctataaaa atttcccctt gaattttctt cccatttaaa aagaaatctt caaaatcact
     3661 aataattgta tttacaactc ctaaatcaaa taatcttctt ccatcaacac ttatcaaagt
     3721 atcataatat atatgatatc catcacatcc aattgttatt gattttaatg cctgggttct
     3781 taaaagttta taatttctct gtcttgcttc gttatctaac catggagtta cttttaaatt
     3841 tccaactctc ttcctgaaat tttccatttc tttttcccag tatttaagag taagcggaga
     3901 taattcatac atacttggtc ctgctatatc aattgaatta tattttgaga gaatctccca
     3961 attacatcta tagtcaggtt cttttatacc tccataatta gcaaatcttg catcaggatt
     4021 agcttgtttt attgaattct ttatttttaa aaatatctgt gcctgttgcc aggatgcaaa
     4081 ttctctccat tcttctttat actttgattt tatcaataaa ggggttaatt cctctttttc
     4141 atctattcca gcatatttcc taaatgattt tatacatcta tcacaaaaac aaatttccca
     4201 tgcattagga ccttcagtat catgccatat atcatttcct ttttcagtta caagtttttg
     4261 aaccatcttt tttgtattta aaaatgcctc ttcatcctca atcattaaag ttgggcatac
     4321 tgtatctatc ccctcatatt ttgctttatc tccattaaaa gtaatcgcat aagattctgg
     4381 atgtttttct aaatattctt tatcccacca aaaccaccat aaagatgtcc taattctaaa
     4441 gcccttttta tttaattcat ctcttatctc cttattattt acacttgtta ttaaaatatt
     4501 aaaaccactt ttctcccaag aagaagcact ttctaaaact aattctttct ttttttcttc
     4561 tttcacagat gcttgaaatg gaccaaaata ccaatcagca aatactcctg attctatact
     4621 ctttggcatt tttgcattaa attccgcgac agtttttact aaatattcat tttctttttc
     4681 aacaattttc ttttcgttat tttcaatatg aaaataaatt ttaaattctt ttaaatttgt
     4741 ttttcctata ctaaaaggta atattaaacg ttcataaatt ttttcctctt cttgagatgg
     4801 aagtgttcta acaggaattg gttttgaaaa ttttaattca tactttttgt attttccata
     4861 ttccttatct atttcttctg ctttttcttc aatcaaaggg aaaaattgtc cataagaaaa
     4921 attgtattgt ctatcaagaa ataaaagttc tatctcctta gggacttcca atctcaaatt
     4981 aaaattttga gggctatttg tataattggc aaatataaat gcaaactgct ggatggtatt
     5041 ttctttaata tatagaattt tatcataaga gagtggccag aggcgactta taagaccact
     5101 ataaccagtt tttttaaatg tttttgaata taaaggaatt ccaatttttg gtgttatatc
     5161 aaaatttata ttgtatcctt tatcaacatt tgctttttca aataaagaaa taaattctct
     5221 tccccctttt tttaaattta attggtatat tctttctatt gtttcatcat ctggaataga
     5281 aactaaaatc cttaatggtt cttttaatat attttccatg tttataattt cactttcaat
     5341 tccaatataa tcctttgatt ttaataatgt tatttttgta ggatatatct tagaggaaaa
     5401 tataatttca ccaaattttt ctggttgatg aaaaccattt gtcccagagg tctcattcca
     5461 agaacttaat tctgaaatat gtcttctttc tctgcagata ttaaatttcc atacatctaa
     5521 gtcaaccgga ctacatccta agatagaaaa ggggattaca acttcaattg accagaaatt
     5581 atttcctata taaccatttg ctttccattt tttaactata cctttattat cctgtttttc
     5641 accttttggg tttattataa attggtaata atcatcttta ccctttggtt gaataaataa
     5701 ctctatacag tcatctctcc atacattttg actccctgtt ttctcttctg cttctaaatt
     5761 tttaatctca ggttcataac acttaactgc taaatatatg ttttctttat cataaaatcc
     5821 ataaaactct gtctgtgcat cagcaaagta tggacttttt aattcattcc attcaaattt
     5881 ataaaaatta ttttcctttt ttaatttctt ccatatttct tcttcaattt tcccatcaat
     5941 ttctattttt tcttcttcat ttatcattgg gtaaactctc tgctcatcta caaaagaata
     6001 ttgtgaaggc aagcagggtg ttaaatttat tttttttact acttctaatg gtatttcatt
     6061 ttcaaaaaca cctattctca aacttattct tgataaaaag tccctctgag ttatttttat
     6121 tccaagtgta ggccctctga aaattctaaa atgatggcag gcggcatcag ggtcaaaatc
     6181 atagtccctt ataattatca atccattttt ttcttctgca ttatacccaa ttaaccacct
     6241 tgaatttttt atttcgattg atttttccca gtaatcaccc aataattcag gagttgtttt
     6301 attttgttcc cagaccagtt ccttagagtc agataaatta aaataaatat ttgattctaa
     6361 actaaatccc tctttttccc tttttatttc tgcaacatag tttataaatg actctttttc
     6421 gtcaaatgtg taatgttcaa ttactgaaat atccggaaaa tttctgaaaa ttatcagttc
     6481 tactttttct ctatctctgt tgatataata ctcaagattc tgaacacctg ttggcaacca
     6541 gtaattaaca acttcatatt tatctttttc ttttgttgta tataatttag taaaaccagg
     6601 aacaatttca catatttttt tcccatctaa attgatagaa acagttccat atgctgattt
     6661 tttattgaaa attaaaactg tttttccttt ttcaaatgaa ataaagtcat tttcttcttt
     6721 aattgaaatt tctgaaaaaa caataaatgg caaaacaaaa ataaataaaa taaaacttct
     6781 tatcttcatt tcttcttcct tttttatttt tttattgtaa aataagcaca gcaatttcca
     6841 aaggttttat ttccacagtt gtttctctaa gtgggttaaa ttttttacct tcagggtaga
     6901 aatatccaga aatttcagta tccatacctg gaaaactaaa actcaattta cgtgtttcat
     6961 tagcactatg gttgaacaaa ataactaaat gtttatttcc caaagtaaat acttcccatg
     7021 aaacattttt gccataaggg ttcttttcta ttttaataat actattgttg tttttacctt
     7081 taaggaaaaa gtcctcaaat ttagcaatta atgcagttgc tcttcctatt tcctggtaat
     7141 aaagtccatc catcggtccc caccaccaga atgaaactcc atctgctcct gaagctactg
     7201 aacgaagaat ttgcatacgg attaaatcag cagaaggttg cattgccgct ccacgttcat
     7261 agccaggagt taaagtaggc actgttttaa ctctatgctt acggggcagt gttccttcca
     7321 tcaattttat aaactctttc catccactta caaatctatc atcaagaaat gaaggactat
     7381 tggaatatcc cgttggataa gcaatatcaa tgtaattggc aatttcttcc caattacatc
     7441 tgtaactttc catagtcatc tgacctggca tcccagaata tactccccaa gttgcattag
     7501 gattattctt ttttaattca tttcttataa cttctgataa catactggac tgatgtgttg
     7561 caaataagat ccattcattt gcatatttct cttttatccc tcgtggagta agttcttctt
     7621 cctgagaaat ttttgcaaat tttctaaact catctaaaca tcttttacaa aaacagacat
     7681 cccatgcccc tggaccctcc aagtcccaaa tataaccatc agttattccg ttagtgacag
     7741 attgagaaaa tcctttaata gattcacgca atacctcacc attgttttct aacagaatag
     7801 taggacagat gcgcggtaag gggctactgt ctctgactcc attaaaagta atagcggtat
     7861 gttcaggatg ttcctttaaa tattcattat tccaccaaaa ccaccagaaa ttgtttatta
     7921 ttatcatatg gtattgttct tttgccatat ctgaataata tgacttatct gtttccacac
     7981 ttatagaatt aaatcctgct ttttgtaaag tatcaagata tggtttccaa atagatgggc
     8041 atgtgttgaa tgtccatagc caattatgta tcaaaatatt tttgggttgt tttccctcaa
     8101 tattaggaag tgtaactaga tacatcatat tttcttcttc ttctatccca tctgcttcta
     8161 atttaaaata tgcaggtatt ttttccccgg cggaaaatac tccattctct attgtaaaaa
     8221 acaatactaa actattctgg aaaaaacgca ctttttctat gcgtgaaggt ggtatattct
     8281 tttcaaggtt aaatgtatac ttatgataaa tttgcccatt ttttgattct tcagaaatat
     8341 tccatgaggt aacattatta tatctttctg gacctattag aagagagccc attgggtcaa
     8401 ggattttaac accagaagga acagatacta caaatatagg attagttatt tgccttttat
     8461 caggaaaatg attggcaaaa agaaaaaaac aatgttgcgc agtattaagt gcaatataaa
     8521 gattcccacc tctcatctca ggtggccatg ccacagacac aagtgactca gaataggttt
     8581 taatggggaa tttaagttca tagagaactt tttcagaatc ttttataaca gcagacaaat
     8641 tgtaaaaacc tggcttttct atagaaaggg gaaatgttat cttttttgtt ttattcccag
     8701 tgatacttat ttccttatta cttttaataa aaacatcttc tccttttgca atagttaaaa
     8761 atatagacaa attttttgta tcgccatcta tcgcttttaa agaaaatgta atatctttaa
     8821 ataattctcc aaaagtaacc gcttcaagag ttactggttt ctgaccaaat gaaataagtc
     8881 caaacttatc tggttgatta aaatctcccc cgacataatt ccaatgactt aattcccctt
     8941 taggacgctg ttctcggttc aaattaaatc cccatatttc gccttctcct ggtttattca
     9001 catcaagcag acgaaatgga atagatgctt caacaaacca cttatcttta cctatagttg
     9061 ttgctacttt ccaatctaac ccttcttccc ctattgcagt taccctatca attcgaacag
     9121 aaagattatc ctgtcgtgtt cctatgggat taacaataaa atgtaatgct tctttttctg
     9181 taaaaccagg gtctataaat atttctaaac aatcatcctg ccaaacatta tcatttcgtt
     9241 tagttctaac ttttcttata aatggtattt gactctctaa catttccgca gcaatataca
     9301 aattattatc atcataagca atatatccta ttgtctgttc agttgccaat ttcccatttg
     9361 taagtgtgaa atctccaagt tttgctgctt tctgccatgc ttcatcattt aaaactccgt
     9421 ctataatagg agggattttt atataaggaa catcggataa acgccgagag gataaaagaa
     9481 atttttcttt ctcattttct ttagtagatt cagatacttt ctcatttttc ttttcaattc
     9541 tttgtttgag aagattctca agaacatcag cattatcaat atctgtaata acaactacat
     9601 ccattctgcc agttgctgtt ggaatattct tgtctatggg cttttgtaaa taaattgaat
     9661 agaaagtttc tccaatacta ttggaaaact tacctatcag cacccatgtc cattcatcag
     9721 gtttatcagt gtgaaaccac ccaaaagccc ttttcttttc actattttca tacaaatacc
     9781 aattagcagg aacatcactt tgtactcttg tccatacatt ataagttttc ccatcctgag
     9841 ggaaaggtat acccataaca aaatgatacc atgttttacc ttccacaaat gctccaccac
     9901 ttgctgaatt acttcctact atatttgaat tacctttgaa atcttctgcc tcaaaccagt
     9961 ggactgtata accatttgca aaaagcacct gacaaaacag aaacataaat aaacaaagaa
    10021 cttgtttcat ttatatctcc tcgtataatg ttcgtaaatg agcatatata agtattttaa
    10081 aaccacccag aataccaata atatagtcgt gttgatgcat gtcctgatgg attatctgtg
    10141 gcatttttat accataaatc attagtggat aatgattcac aatggccgtc aaccatcaaa
    10201 acatttgtca taccatgatg ccttggtgaa aaagttcccc aggtagaagg tgtagcataa
    10261 ggtggccaaa cagttgaatc atattctata taacctccag aagtagaagt atatatctgg
    10321 tcaagagatg ggttatattc accttttgga tctgcattat attttccatc acataccata
    10381 acaattcttt cctggtgtct tacttttgaa agtttaactg gtttaaatgt atcttgggtt
    10441 gtaggatata accaataatc cctaaaaaaa tatacgctaa ttccatagga aggacctgca
    10501 tactttgctc tttgacttgg gcatctcatc ggacctcttg aaccaccgta ttcgtattga
    10561 gcatctttat aacctaaata aggagcaagt gctctatgaa gacacctttt tggccaacca
    10621 tagggaaaat attcattata atcatttaga tacatataca ttgcggtccc tatttgcttt
    10681 aagttgttca tacatacagc agaccttgct ctctctcttg cttgtgataa agcaggtaat
    10741 aacattgatg ctaaaatcgc tataatcgct attaccacaa gcaattcaat gagagtaaac
    10801 cccgtctttt ttctcattct tatcacctcc tttgttctcc ctaaactcct tcctttttca
    10861 aaggaaggtt gggatggatt taaaaatctc ccctcgcccc ttccgccaca aagcatgtcg
    10921 gacagatgct ttgttaaaga ggggaacttc tttcttattt ctcttaccat ttaacacctc
    10981 cgtttccgcc ataaagcgcg gcggatgcta aaatacactt tttcctctct ctttattaaa
    11041 gaggggaatt tcttatcatt ttctcctttt catcacctcc ttttcacact ttctcttatt
    11101 ataagttttg tttcaaggat tacctgtttt ctttttttat tctttttttt cttttcttct
    11161 tcaagtattt caaatccttt ttttgccaat aattcaattg gttgataaat tgttgttaat
    11221 gggggagtaa tatgtgaaca tattgggaac tcatcaaaac ctattaaaga aatatcatct
    11281 ggtatttttt tatctgcttt ttttaaagca tctattaagt acaaagaaag agttatacca
    11341 ccagttataa atgcagttgc taaatttttc tcttttaaat tttcctccaa aattttttct
    11401 acctcttttg accagtcaaa attactttcc agaattactt cgttatgttg tattttcctt
    11461 tttttacaat aattcataaa ttcttcatat ctttcataag tatcatgact ttcaatacta
    11521 cctccaataa aaaatatatt tttatgtcca agagaaataa gatattcaac tgcctgtctc
    11581 atcccacctt tattatcaga agcgacatag tttatatctt ttctttctaa atttgctcca
    11641 attacaacag atgggattga atatttactt aacctattta taagttcatt gtcatttctt
    11701 tttggattta gaactataat tccagaagat tctttcgtca cttctgcata ttctatttct
    11761 tctttctgcc agttttttaa gagtattgta tatccatttt ttgttgcttc ttttcttaac
    11821 tctcttaata aaatatcttc ataagtattt tgggatattt ctgattcatt gtgaaaatnn
    11881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc tataaagagt aaaaacaaag
    12001 ggtatttggg aagacaaaaa aaaaggataa taaatttcgt attctgaaaa ggtatgggaa
    12061 agtggacttt cccatattta taataatgta ttttcgtatt gggggtatcg ttgggaagag
    12121 gggaagcacc cctgtcccaa gagcgaaggc gacgaatacg ggttgattcc cgtaccccgc
    12181 tataaagagt aaaaacaaag ggtatttggg aagacaaaaa aaaaggataa taatttccgt
    12241 tttctgaaaa ggtatgggaa aagagtaaaa gcaacgaata cgggtttccg ccaaaagacg
    12301 cgtcggataa actatatccc ttaccccgct ataatatgaa ggagataaaa tgaaagttga
    12361 aataataaaa aaatcagaat taggtagaga attgaatttt atagttgaaa aggaagttgt
    12421 tgatgaaaca aaaagaaaaa tattagatga aataaaaaaa gatgctgaaa tagatggatt
    12481 tagaaaaggg aaagcaccag atgacttgat agaaaaaaag tactcttcag caattcagga
    12541 acaacttttg agaaaattaa tcacagattc atatataaat gcaataaaag aaaataattt
    12601 taatcctatt gttgaacccg atgtttatga tgcgaaattt gatgataatg gaaatttatt
    12661 gtttaaaata tacatagagg aaagaccgga tgtcaaaata gagaaatata aagaaatacc
    12721 tgtaaaaaaa gtaaaaccaa aagaagttac agagaagatg gttgaagatg ttcttaaaga
    12781 atgggaaaaa acgcctgaaa tgaaggtttc tcttatagac attgaaaaaa gaaaagcgtg
    12841 gaaggaaaaa gcaagagaac aattaattgc ttatagtaaa gcaagagcag agatggaaga
    12901 agagaaacag ttatgggatg aacttatgaa aaatataaag ttccctgttc ctgaaaaact
    12961 tgtaaatgaa agagcaataa gatatacaga agaacaatta aaagcaatga atcttgagaa
    13021 taaatcaaaa gaagaagttg aaaaaatagc aaaagagatt tttcaaaaag taaaaccaat
    13081 ggcagaagaa gatgtaaaaa aatattttat tttggaaaaa atcagcgaat tagaaaatat
    13141 aaatgttgat gaaaaagatg tggaagaaag aattgagcat ataagcaggg ttataggaca
    13201 accatatgaa aaaataaaag agcagataga gaaaaatggt gaaatggaaa atttaaagga
    13261 agaaataaaa attgataaaa cattcaggtt tataaaggaa aatgcaaata tgatagaaag
    13321 aattatctta ccaggagaaa aataaaatgg aaaagaaaaa tgaaataaaa aatcaggtta
    13381 taccatatgt tattgaacaa acagaaagag gagagagagc atatgatatt tattctcgac
    13441 ttctgaaaga tagaataatt tttataggta ctgctataac ggataatgta gcaaatgttg
    13501 taattgcaca atttttattc ttacagagtg aggactcagg aaaagatata catctttatt
    13561 taaatacgcc tggcggagca attactgctg ggcttgcaat ttatgataca atgcagtttg
    13621 tttcctgtga tgttgatact tattgtattg gacaggcagc aagtatggga gcaattttac
    13681 ttgcaggtgg aacaaaagga aaaaggtttt tattgcccca tagcagagtt atgattcatc
    13741 aaccatgggg aggagttgaa gggactgcaa cagaagtaaa tattcataca agagaaatat
    13801 tacggcttaa aaaaattgta aatgaaattt tatctttaca tacaggacaa tcaataaaga
    13861 aaatagaaag agatactgaa agagattatt ttatgagtgc agaagaagca gtgaaatatg
    13921 gaattagtga taaaattata caatcagaaa aggtaaagaa actataaaaa taaaaaatgg
    13981 ctataattaa aaagaaaatt gttaaaaata ataatcctgc aattccatta agagaccttg
    14041 ttatttttcc aaatatgatt gtacctcttc ttgttggaag aactcgctca ttaaattcaa
    14101 ttgaagaggc attaaataat gacaaatatt taattgttgt ttttcagaaa agtccactta
    14161 ttgaagagcc aacatttgat gatatacatt caacaggagt tgttgtaaaa attatgcaga
    14221 gtataagaga accaagtgga acgatgaaaa ttcttgttga ggttattaaa agagtaaaaa
    14281 ttaaaaattt tataagtaat aaaaaatatt atattgcaac agttgaagaa ataagggaag
    14341 aaacacaggt aaaagataag gaaacagaag cacttatgcg cctgctgctg gaacttgttg
    14401 aaaaatatat tgacctaaat ccttctgttc caaaagaaat ctatagcaca atatctgtaa
    14461 tagaagaacc atcaaaactc tgtgatacaa ttgcaggtta tttaccatta caagtaaaag
    14521 ataaattaaa agttcttgaa actataccag taaaagagcg gttgaaggtt cttattgata
    14581 ttttcaataa tgaaattggt atacttgaag ttcagaaaca aatacaggga aaagttatta
    14641 aaaaaattga ggagactcaa aaacaatatt ttcttactga acaattaaaa gaaatagaaa
    14701 aagagttagg acaggaggga gaaggtcctg aaataaaaca attaaaagat gcgataacaa
    14761 aggcaaaaat gccaaaaaat gtagaagata aagcactgga agaattatcc cgcctatcta
    14821 aaatgatgcc tatgagtcct gaggcaacag ttatcagaac atatttagaa tatcttataa
    14881 atttaccatg gagtgtttca actgaagata atacagatat ttcaaatgca gaaaaaatat
    14941 tgaatgaaga ccattatggg cttgaaaaag ttaaagaaag aattttagaa tatcttgcag
    15001 taaagaaaaa gacggattct ttgaaaggac cgattttatg ttttgttgga cctcctggag
    15061 taggaaagac atcattagga aaatcaattg caagggcttt gggaagaaaa tttgtaagaa
    15121 tttctttagg aggtataaga gatgaagcag aaataagagg acatagaaga acttatatag
    15181 gtgctttacc aggtagaatt attcagtcat taagaaaagt tggagtgaac aatcctgttt
    15241 ttttacttga tgaaattgat aaaataggaa ctgacttcag gggagacccg tcttctgcac
    15301 ttttagaagt tcttgaccct gaacaaaacc actctttttc ggacaattat ttagaagtgg
    15361 aatttgactt atctaaaatt ttatttataa cgactgcaaa taacccatat acaattccag
    15421 caccacttct tgatagaatg gaaataattt ctattgatgg atatactatc tgggaaaaaa
    15481 agggaattgc aaaacatttt ttaattccaa aacaaatgaa ggaacattct ttgggagacg
    15541 atgaaataaa attttctgag gcatctataa ttaaaattat aaaaaattat acaaaagaag
    15601 caggtgttag aaatttagag agagaaatag caaatgtttg tagaaaagtt acaaaaaaaa
    15661 tagttcagga aaataaaaaa aggaaatata tcataacttc aaaaaatctt gaaaaattta
    15721 ttggaattga aaaatttact gaaacagaag ttgaaaaaga accgaaagtt ggagttgcaa
    15781 caggtatggc ttggactgaa tatggtggag aaatactttt tacagaggcg ttaacaatga
    15841 aaggtaaagg caatcttatt ctgacaggac aattagggga tgtgatgaaa gaatcagcac
    15901 aggcagcatt gagttatgta aggggtcatg cagaagaatt tggaataaat cctgattttt
    15961 acaaagatat agatatccat attcatgtac cagaaggagc aattccaaaa gacggacctt
    16021 cagcaggagt tacaatagtt acctctcttg tttctgtttt atcaggaaaa ccagtaagag
    16081 gtgatgttgc aatgacagga gaaataactt taagagggaa ggttttaaaa attggtggac
    16141 ttaaatcaaa aattcttgct gctcatattt caggaataaa aaaaataata ataccaatag
    16201 aaaataaaaa agaccttatt gacctaccaa aaaaaataag agatgaactt gaaataatac
    16261 cagttgaaac tgtaaaagat gtccttgaaa aagcattaat ccaataaa
//
LOCUS       DAOOVW010000036        16245 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_2414_length_16245_cov_9.548301, whole genome shotgun sequence.
ACCESSION   DAOOVW010000036 DAOOVW010000000
VERSION     DAOOVW010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16245
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2414_length_16245_cov_9.548301"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..214)
                     /locus_tag="PLW95_05840"
     tRNA            complement(141..214)
                     /locus_tag="PLW95_05840"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(178..180),aa:Val,seq:gac)
     gene            complement(461..1240)
                     /locus_tag="PLW95_05845"
     CDS             complement(461..1240)
                     /locus_tag="PLW95_05845"
                     /inference="COORDINATES: protein motif:HMM:NF022135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HOV22185.1"
                     /translation="MRKFIFLYVFLFCGICFAHFGMIIPDVDYVGNKNESTIGVKIMF
                     AHPFDGQTITMERPEEFGVIVNGDKVSLINKLKETKVTGYNEKDGHTGYFLKYTLEKP
                     GDYIFYFISKPYWESADDKYTIHYTKVIVNGFELQDTWYKEIGLKTEIVPLTCPYTIY
                     KGNTFSGIVKVDGKVAPFTDVKVEYYNENGRAKIPNPLFETQIIKCDGNGVFSYSFPE
                     SGWWGFSAVNTDDKNEIPYRDKLKKVEIGADLWLRVHIFRK"
     gene            1387..2175
                     /locus_tag="PLW95_05850"
     CDS             1387..2175
                     /locus_tag="PLW95_05850"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOV22186.1"
                     /translation="MGEKIGVIHYNTGDKNLEEFLSWSNRNDIGYVELQRKDVWNEEI
                     KTDMVGKLLNKYKIKISQISCGNDFLQKTDDEFQKQVKIVETMCDIVKELGFNHLRID
                     GGWPKEGVEEKNYKNLVMKGVAKVVEIAEKKGLYLALDNHGTVTNNYLLQLEIFENIK
                     SKFLGANLDTMNYRWFGYPVEKLTEIYKAIAPYAIHTHLKDGTGSKENYVGKVLGEGE
                     IPVIEAINILKENGYNGVWCIEYEGKDQNIGYEKCFEFLRNLFK"
     gene            complement(2216..3244)
                     /locus_tag="PLW95_05855"
     CDS             complement(2216..3244)
                     /locus_tag="PLW95_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22187.1"
                     /translation="MLSKRIKYEDFLEIKGKSLLEFKDGNSILCIAPNIGGRVFAFMD
                     GLSLHRIDLENIKNPNRPFNNFGGGNLWPAPEGGKFGFNYRGNEWYVQECINSQPYNI
                     LLYNKNSAVIEKEIVLVNRSNTIFNVIMKRKISICDIPPFFKEWNISNSITYISEDSF
                     EVLNDVRVENGLIAAWTLEQFDANENTISFCLVNSPENAINFDFYEPPIEKIVYYKKG
                     FTYQTDGMKRGQIGIKRKSNPKAIGFIDFEQKIVCIRQNLSSTKNGLYFNIADNEQPG
                     GVFSAEDTYSIFNSSEDMRFFELETIGCSDINCGKVIGSTLVSLTSFAFFENIEELKT
                     WKFLDEVI"
     gene            complement(3375..4178)
                     /locus_tag="PLW95_05860"
     CDS             complement(3375..4178)
                     /locus_tag="PLW95_05860"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOV22188.1"
                     /translation="MGNKIYLSPSTLNIYRECPKCFYLHIHYGIQRPRGPMPSITIGL
                     DSIIKKYFEFYRNIGQLPPFLEDKIEGKLITILEKTYYYDINNKYCIFGKLDEAIALN
                     DGTFVPLDHKTRASAPDKVHEAYQLQMSVYTLLLRQNNKKVSNFAYLVYYYPEESKIH
                     NGISFGFKVEKVETDPDEVIKYINEAIECLESPKIPPGGKNCEYCKWVNQIKEYYFFD
                     EVPRIEKNKKLPKIEKKENTETKKDIAENKEKEEDKKKLEENFEKGFLF"
     gene            4297..5103
                     /locus_tag="PLW95_05865"
     CDS             4297..5103
                     /locus_tag="PLW95_05865"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HOV22189.1"
                     /translation="MLTSIMVVAGLGIVFASFLTYFYVKFQSEENPLVSKIQQLLPGA
                     NCGACGFAGCNGLAEKLIEGKTTPDKCVLISSENLEKICSLLGIDNMEKKEKKVAKLF
                     CFGGINAKKKFEYSTIKSCSAVKSLFTTNYECQYGCLGYGDCVEVCPVQAINIGENNL
                     PEIDNDLCTGCGKCVEACPKNIIRVIPYGKNVYISCSSYDKGPKVIRACKSGCIGCGK
                     CVNICPKEAIKIENNLAIIDYEKCDNCEKCVSECPRKIIFVSNKKVEIAV"
     gene            5104..5922
                     /gene="truA"
                     /locus_tag="PLW95_05870"
     CDS             5104..5922
                     /gene="truA"
                     /locus_tag="PLW95_05870"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOV22190.1"
                     /translation="MRNIKLVFSFDGSKFSGWQKQKNKRSVQETLENTLEKITNEKIK
                     VIGCGRTDSKVHSINYVANFKTNSKIPSENLKKAINSFLSPEVYIKKVKDIPLSFHSR
                     YDALRKSYIYLISLSKCPFLNNYALFFEREIDIEKIIEGKEYIIGKHDFSAFKGEGSN
                     NKNPVREIFKIDIKKKRFFLDRDVKLIEIEIEGDGFLYKMVRNIVGTLIYTGIGKIQP
                     SDIKKIIEGKNRKFAGPTACASGLYLKKVVYPKIINSPLSSLKGKNKKINEYKR"
     gene            5906..6802
                     /gene="murB"
                     /locus_tag="PLW95_05875"
     CDS             5906..6802
                     /gene="murB"
                     /locus_tag="PLW95_05875"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOV22191.1"
                     /translation="MNIKDEFKNFLSERVKENTLLSQFTSFKTGGIARIIVFPENKEE
                     IKKIFHLSEKYQKDILILGKGTNILISDYGFDGVIISTLLLKKITKKENFIISESGVL
                     ISSLLNFCLKNNSGGIEFLSGIPGTIGGALITNAGLKKEWIGEKVEWVEIYDKKKKDF
                     EILKREEIKFSYRDSNLKNMFIINACLRTEKKRKEEIKNKIQNYMKERIEKQPLRENS
                     AGSVFKNPENFYAGELIEKCGLKGYCIGDACISERHANFIINKGKAKSSDIYNLIKII
                     QNKVKEKFRVELELEIKIIGKF"
     gene            6804..8135
                     /gene="tilS"
                     /locus_tag="PLW95_05880"
     CDS             6804..8135
                     /gene="tilS"
                     /locus_tag="PLW95_05880"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOV22192.1"
                     /translation="MELFKKCEKFVSEKGIIENRDKILVSVSGGPDSIFLLYFLLYLR
                     NKYNFSLFVSYIHHHLRKQADKEFMYVKEISKKYGIPFYYRNIKIEGKTGIEEKSREK
                     RYKALYQIAKKTGVNKIATGHTLDDNIESVLLNFFRGTGLAGMSGILPERKIYKNSNI
                     FLIRPILTVRKSEIKKFLNNKGIKYYLDKSNLNLKYRRNLIRYKIIPYLKKFFPSFTD
                     IIAKTSSILQDDFLYIEKEAIKTLELILKNGQFNVSEFKKIDISIRRFIVQLLFEKIA
                     GVSYRSYNKIENLRNFIEGIKESYVPISIIEERIKGEKKERKIYKVIEIPGKYNVDGI
                     FIESEYIKKSPFIFKNMDKYTGYFDGEKVGKKIIVRSRKKRDMFQPLGMQKEKKLSRF
                     LIDRKIPIYKRDKILIFQSKNEIMWVCGVEISEKFKITEETKKILKIKVNV"
     gene            complement(8180..9697)
                     /gene="guaA"
                     /locus_tag="PLW95_05885"
     CDS             complement(8180..9697)
                     /gene="guaA"
                     /locus_tag="PLW95_05885"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOV22193.1"
                     /translation="MVGVIDFGSQYTQLIARRVREMNVYSEIFLPNIKIETLKEKDIK
                     AIILSGGPGHIQKGIYKFDERIFDGSFYVLGICYGMQLISEFFSGKVEEGKKREYGKT
                     IFYPDRKEEIFNNLKEKTIVWMSHWDIVKKTGNNFKIIGRTEISPITAIRDKNKKIYG
                     LQFHPEVTHTEEGKKIISNFLFKICKLEPTWNYGIILEKIKKEIKEKVGDEKIICGLS
                     GGVDSSTLSLILQDVCKEKSLSIFVDNGLLRKGEKEKIVKWFQKKVNLKYIDAGEIFL
                     KQLKNVADPEKKRKIIGNTFIKIFEKEAKEFGAKFLAQGTLYPDVIESIPAFGGPTAR
                     IKTHHNVGGLPEKLKFSLVEPFRFLFKDEVRKIAKEINIPDFIINRHPFPGPGLAVRI
                     IGSVNKHLLEILGEADTIVEDEIKKASLYNSMWQAFAVLLPIKTVGVMGDKRTYENVI
                     AIRVVKSTDGMTADWAKISYKVLDRISTRIVNEVRGVNRVVYDITSKPPATIEWE"
     gene            complement(9766..11097)
                     /locus_tag="PLW95_05890"
     CDS             complement(9766..11097)
                     /locus_tag="PLW95_05890"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HOV22194.1"
                     /translation="MNNNLNKDDVLKIVEKNDIKFIRLFFADITGQMKGFSITNEELK
                     VALEDGMGFDGSSIKGFARIDESDMIALPVPSTFTILPYRPQERGESAMICDILNPDR
                     SPYEGDSRYILKKQLKKLKEKGFSYNVGPELEFFIFKDDNNPQILDEGGYFDITTLDA
                     GNNVRREVILTLEKMGIDVEYSHHEGAPSQHEIDLRYKEGLEMADIVMVYRMVVKDVA
                     QANGLYATFMPKPIYGVNGSGMHVHQSLFKGDKNVFFDKNDKYNLSDIGKKYIAGILN
                     HSREITFVCNQWVNSYKRLVPGYEAPVYICWARRNRSSLVRIPMYKPGKEKSTRVEFR
                     SPDPACNPYLSFACMSAAGMRGIENNYQLPEPLERDVYHLTDEEMEQLNIKCLPGSLI
                     EAIEVAEESKFLKEVLGDHIYNNLITAKKIEWDMYRQQITDFEIKTYFPTL"
     gene            complement(11110..11946)
                     /locus_tag="PLW95_05895"
     CDS             complement(11110..11946)
                     /locus_tag="PLW95_05895"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOV22195.1"
                     /translation="MFKNLAPGALGINKSLRECLRLATIGEFEGMDLPVDETIELVEK
                     YSVDYVKGMYSSFNLKVGGWGLPIDIYSEENFKKGIEKLKKFVKIASEIEAERIYTWI
                     KPYSDEMEYNENFKWHRKIIKKITEVIGGCKIGFEFIGTPSFRKDHKYPFVHTIEQML
                     DLVSDFENAGILLDSWHWFTSGGSLEDIKKLDKNKIIYVHINDAPFSDIEKLVDTERN
                     IPGETGIIDLTSFLKTIKETGYDGPVTPEPFNRRVNELPDEIAVRLVGGYTTKLWENI
                     FK"
     gene            complement(12106..12360)
                     /locus_tag="PLW95_05900"
     CDS             complement(12106..12360)
                     /locus_tag="PLW95_05900"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOV22196.1"
                     /translation="MEEFKEKEGKVHIHLCGNAGHLFKFLKENLDVISFDTGFPVNFR
                     KLREELGEEIEIIGGPNIVLLKNGKENEVKDEVKKYVKAE"
     gene            complement(12368..12541)
                     /locus_tag="PLW95_05905"
     CDS             complement(12368..12541)
                     /locus_tag="PLW95_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22197.1"
                     /translation="MSFVTENIIKRMTEIRKWRAEKEEKAEINFKSKIFSFADDLIAN
                     LSVEKYKNFVFFS"
     gene            complement(12612..12896)
                     /locus_tag="PLW95_05910"
     CDS             complement(12612..12896)
                     /locus_tag="PLW95_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22198.1"
                     /translation="MRIPSDGWQINVDFQNNYESGWFGCEIIYLGNFIPPDVNPIFKE
                     NKHRLYEIEPPYPLKGNLLGKAMEFFEYMNRRYKDIEFEGKKVKGPLTIP"
     gene            complement(13348..14406)
                     /locus_tag="PLW95_05915"
     CDS             complement(13348..14406)
                     /locus_tag="PLW95_05915"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HOV22199.1"
                     /translation="MIIGVPKEIKVEEYRVGLIPSFVKDIIKDGHKVFIQENAGVGAG
                     FSDNDYKEVGAEIVKGLEDIYKKCDLIVKVKEPQEEEIKLLSEGQTLFTFCHFSSNRN
                     MTEKLIEKKVNCIAYELVEENGNLPILKPMSEIAGKLSIQEGMKYLEKEYCGKGILLS
                     GVENVESGNVLIIGGGVVGYSAGIIAEGIGAKITFLEIAENKIKILKERFKKADVLYS
                     DRENLISSLKKADVIVGAALIPGKRTPVIIKEEDLDVIEKGSVIVDVAIDEGGLCETS
                     KSTTHKNPIFIYKDIIHYCVPNIPGVVPKTSTIALNYATFPYLKEILKGEENWFVNKP
                     IEEGLVIYKGKLRREILF"
     gene            complement(14403..15710)
                     /locus_tag="PLW95_05920"
     CDS             complement(14403..15710)
                     /locus_tag="PLW95_05920"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOV22200.1"
                     /translation="MNKKKVSDFRYDTGNVAVPWAAVGESTNIDDIEEILKFLIQPVD
                     NKNYKKQLKNVKKELKNLLKYGRYATKLTLGNKVLELEEYVCKFLNVKYACFITNATA
                     GFEIGLKFSGLKPGDEVIAPAITFCSTISYPLLIGAKVVLVDIDRRTINIDPDDIERK
                     ITKKTKAIIPVHIGGYPCDMARIMKIAKTYNLKVVEDAAHAFGGWYKRKAIGTIGDFG
                     SFSFHEVKNVTSLGEGGIVVTNSEIGKDFPKARFVGFDSSNPIEHWLYDVVALKRESD
                     YIPAGNHSTTEIQAVALLSQIKRIEKIIKERRKNAEFLNSCFKDVDGIITPPLDTYDI
                     TPTHHLYLIQIEPEKLKGDIQLFKQKLTEKGVTQIQHFAPLYRFSYLKQLGYDTEKIK
                     KSCPNAEDLFLHRFTHLPLYPLTKKQVEYMADKVIETVKEMKK"
BASE COUNT         5612 a         2549 c         2096 g         5988 t
ORIGIN      
        1 gagcctttcc caacgatacc cccaatacga aatacattat tataaatatg ggaaagtgaa
       61 ctttcccata cctttttggt cacaggtaac actctccatt tccaatattc ttatcctatc
      121 tttactttta cttctttcaa tgggcggtac aggacttgaa cctgtgacct ccacgatgtc
      181 aacgtggcac tctaaccaac tgagctaacc gccctttccc ttattttata tttttttatc
      241 ctttttgtaa ataaaccgta tgagttgagg acatcagtaa cactttttta tctaaatttt
      301 gttgtttttt catctcaaat cttgttgctg tctctgtttt atcatttaca tttttgtata
      361 ttttaaaagt atgataaaga atgggaagag aacatttatc ttttaagtat taagtattct
      421 attttttaaa caacaaatag gtggtgaaaa tttccagatt ttactttcgg aatatatgaa
      481 ctcttaacca gaggtcagca cctatttcaa cttttttcaa tttatctcta taaggtattt
      541 catttttatc atcagtattt acagcagaaa atccccacca cccactttca ggaaaagaat
      601 atgaaaaaac accatttcca tcacacttta taatctgtgt ttcaaataaa ggatttggta
      661 tctttgctct accattttca ttgtaatact caactttcac atcagtgaaa ggtgctacct
      721 ttccatcaac ttttacaata ccgctaaaag tatttccttt ataaatagta tatggacagg
      781 ttaaaggtac tatctctgtt ttaagtccaa tttccttgta ccatgtatcc tgcaactcaa
      841 atccattaac aataactttt gtataatgaa tagtatattt atcatctgct gattcccaat
      901 acggctttga aataaaataa aaaatataat cacctggttt ttctaaggta tattttaaaa
      961 aataccccgt atgcccatct ttttcattat atccagttac cttagtttct tttaatttgt
     1021 ttataagaga tactttatct ccatttacaa taactccaaa ttcttctggt ctttccattg
     1081 ttattgtttg cccatcaaaa ggatgagcaa acataatttt aaccccaatt gtactttcgt
     1141 ttttatttcc aacataatct acatcaggaa taatcatacc aaaatgcgca aaacaaattc
     1201 cacaaaacaa aaaaacataa agaaaaataa atttcctcat tagaaatctc cttattgtta
     1261 attataaatt ttacccatta aattataaaa taaattttat ttttttcaaa aattttgaca
     1321 ggaactttaa aaaattataa tcttataaaa ataattttgg cagtattcaa caaaaaggag
     1381 aagaaaatgg gagaaaaaat aggagttatt cattacaata caggagataa aaatttagaa
     1441 gaatttttgt catggagcaa tagaaatgat attggttatg ttgaattaca gagaaaagat
     1501 gtctggaatg aagaaattaa gacagatatg gttgggaaat tattgaataa atataaaata
     1561 aaaataagtc agataagttg tggaaatgat tttcttcaga aaacagatga tgaatttcaa
     1621 aagcaggtaa aaattgttga aacgatgtgt gatattgtaa aagaattggg atttaaccat
     1681 ttaagaattg atggtggctg gccaaaagaa ggagttgaag agaaaaatta taaaaattta
     1741 gttatgaaag gagttgcaaa agttgttgaa attgcagaaa aaaaaggttt atatcttgct
     1801 cttgataatc atggaactgt tacaaataat tatcttttac aattagaaat ttttgagaat
     1861 ataaaatcaa aatttttagg agcaaatctt gacacaatga attatagatg gtttggatat
     1921 cctgttgaaa aattaactga aatatataaa gcaattgctc cttacgcaat tcatacccat
     1981 ctaaaagatg gaactggctc aaaagaaaat tatgtaggaa aggttcttgg agaaggagaa
     2041 attcctgtta tagaagcaat aaatatatta aaagaaaatg gatataatgg tgtttggtgt
     2101 attgaatatg aagggaaaga ccagaatatt ggttatgaga aatgttttga gtttttaaga
     2161 aatttattta agtaagattt gaataaaagt tctatttatt tttcgtatta ttatttcata
     2221 taacctcatc aagaaacttc catgttttaa gttcttctat gttttcaaaa aaagcaaatg
     2281 atgtaagaga aacaagagtg gaaccaatca ctttaccaca atttatatct gaacaaccaa
     2341 ttgtttctaa ttcaaaaaaa cgcatatctt cagaagaatt aaaaatacta tatgtatctt
     2401 ccgctgaaaa aaccccccct ggttgttcat tatctgcaat gttaaaatat aatccatttt
     2461 ttgtagagga tagattttgt cttatacata caattttttg ctcaaaatca ataaatccaa
     2521 ttgcttttgg gttagacttt ctttttattc caatctgtcc tcttttcata ccatcagttt
     2581 gatatgtaaa tcccttttta taataaacaa ttttttcaat tgggggttca taaaaatcaa
     2641 aatttatggc attttctgga ctattaacca aacaaaatga aattgtattt tcattagcat
     2701 caaattgctc cagtgtccat gcagcaatca atccgttttc tactctaaca tcattaagta
     2761 cttcaaaaga atcctcggat atgtaagtaa tagagttgga aatattccat tctttgaaaa
     2821 aagggggtat atcacaaata gaaatttttc ttttcattat tacattaaaa attgtgtttg
     2881 aacgattaac aagaactatc tctttttcaa ttacagcaga atttttgtta tataatagga
     2941 tattatatgg ttggctattt atacattcct gtacatacca ttcatttcct ctgtaattaa
     3001 atccaaattt gccaccctca ggtgcaggcc agagattgcc acctccaaaa ttattaaatg
     3061 gtctattagg atttttaata ttctctaagt caatcctgtg cagagacaat ccgtccataa
     3121 aagcaaatac ccgccctcca atattcggcg caatgcataa tattgaattg ccatctttga
     3181 attccagaag tgatttacct ttaatttcta aaaaatcttc atattttatt cttttggata
     3241 acattatatc ttcctatttt gttaaaatac tttaaagtgt tcccatatat cacaatttta
     3301 attatatctt gagtcttctt ttctaaatta aaagagatta gaacaatgat ttgtgaaaat
     3361 atctattgga taagtcaaaa gagaaaacct ttttcaaaat tttcttcaag tttcttttta
     3421 tcttcttctt tttccttgtt ttctgcaata tctttttttg tttctgtatt ttcttttttc
     3481 tctattttag gtagtttttt gttcttttca attcttggaa cttcatcaaa aaagtagtac
     3541 tcttttattt gattcaccca tttacaatat tcacaatttt ttccacctgg cgggatttta
     3601 ggactttcaa gacattctat tgcttcattg atatatttta ttacttcatc tgggtctgtt
     3661 tcaacttttt ctaccttaaa gccaaaagaa ataccattat gtattttact ttcttctgga
     3721 taataataaa caaggtaagc aaaattgcta accttcttat tattctgcct taaaagtaaa
     3781 gtataaacac tcatttgcag ttgatatgcc tcatgaactt tatcaggtgc acttgccctt
     3841 gttttatggt caagaggaac aaaagttcca tcattaagag caattgcttc atctaactta
     3901 ccaaaaatac aatatttgtt atttatatca taataatatg tcttttcaag tattgtaatt
     3961 aattttcctt ctattttatc ttcaagaaaa gggggaagtt gtccaatgtt tctataaaat
     4021 tcaaaatatt tttttataat actatcaagc ccaattgtta ttgaaggcat tggccctctt
     4081 ggtctttgaa ttccataatg aatatgcaaa tagaaacact tcggacattc tctataaatg
     4141 tttaatgtgc ttggtgataa ataaattttg tttcccattt ttaatattat aaactaaaat
     4201 ttaaagtttt aaaagaaatc ttaccctaaa ttaaaaattt gtggtaaaat taacaaaaat
     4261 aataaagtaa aaaggactat tacaggagaa aaaaatatgt taacttcaat tatggttgtt
     4321 gcaggacttg gcatagtttt tgcttcattt ttaacttatt tttatgtgaa atttcaatca
     4381 gaagaaaatc ccttagtttc aaaaattcag caattactgc ctggagcaaa ttgtggtgct
     4441 tgtggttttg ctggttgtaa tgggcttgca gaaaaactta tagaagggaa aacaacccct
     4501 gataaatgtg ttcttataag ttctgaaaat ttagaaaaaa tttgttcatt acttggaatt
     4561 gataatatgg agaaaaaaga aaaaaaagtt gcaaaattat tttgttttgg aggaataaat
     4621 gcaaaaaaga aatttgaata cagcacaata aaaagctgtt ctgctgttaa atcacttttc
     4681 acaacaaatt atgaatgtca gtatggatgt cttggctatg gagattgtgt tgaagtatgt
     4741 cctgtacaag caataaatat tggagaaaat aatctacctg aaatagacaa tgatttatgt
     4801 actggatgtg gcaagtgtgt ggaggcatgt ccaaaaaata ttattcgtgt tatcccttat
     4861 ggaaaaaatg tttatatttc ctgttcttct tatgataaag gaccaaaagt tataagagct
     4921 tgtaaaagcg gttgtattgg atgtggcaaa tgtgttaata tatgtccaaa agaagcaata
     4981 aaaattgaaa ataatcttgc aattattgat tatgaaaaat gtgataattg tgaaaaatgt
     5041 gttagcgaat gtccaagaaa aataattttt gtctcaaata aaaaagttga gattgctgtc
     5101 taaatgagaa acatcaaact ggttttttct tttgatggaa gtaaattttc tggctggcag
     5161 aaacagaaaa ataaaagaag tgtccaggaa acattagaaa atacattaga aaaaataaca
     5221 aatgagaaaa taaaagtaat tggatgtgga agaactgata gcaaagtcca ttctataaat
     5281 tatgttgcaa attttaaaac aaattcaaaa ataccatcgg aaaatcttaa aaaagcaatt
     5341 aattcttttc tttcaccaga agtttatata aagaaagtaa aagatatacc tttaagtttt
     5401 cattcaagat atgatgcttt aaggaaatca tatatatatc taatttccct ttcaaagtgc
     5461 ccatttttaa ataattatgc actttttttt gagagagaaa ttgatataga aaaaataatt
     5521 gaagggaaag aatatataat tggtaaacat gatttttctg cttttaaagg tgaaggtagt
     5581 aataataaaa atcctgtaag agaaattttt aaaattgata ttaaaaagaa aaggtttttt
     5641 cttgatagag atgttaaact gattgaaatt gaaatagaag gggatggttt tttatataaa
     5701 atggtaagaa atatagttgg aactttaata tatacaggaa ttggtaaaat ccaaccgtct
     5761 gatataaaaa aaataattga aggtaagaac agaaagtttg caggtccaac tgcttgtgca
     5821 tctggacttt atttgaaaaa ggttgtctat cccaaaataa taaattcacc cttgtcctcg
     5881 ttaaagggga aaaataaaaa aataaatgaa tataaaagat gaatttaaaa attttttatc
     5941 tgagcgagta aaagaaaata ctttattaag tcaattcaca agtttcaaaa caggtggaat
     6001 tgcaagaatc attgtatttc ctgaaaataa agaagaaatt aaaaaaattt ttcatttatc
     6061 tgaaaaatat caaaaagaca tactgatttt gggaaaagga acaaatattt taataagcga
     6121 ttatggattt gatggggtta taatttcaac tttactttta aaaaaaatca caaaaaagga
     6181 aaattttatt atatctgaaa gtggtgtttt aatctcttcg cttctgaatt tttgtctgaa
     6241 aaataattca ggaggtattg aatttctatc aggcattcca ggaacaatag gaggtgccct
     6301 tataacaaat gctggactga aaaaagagtg gattggagaa aaggttgaat gggttgagat
     6361 ctacgataaa aaaaagaagg actttgaaat attgaaaagg gaagaaataa aattttcata
     6421 tagagatagt aatttaaaaa atatgtttat tataaatgct tgtttaagaa ctgaaaaaaa
     6481 gagaaaagaa gaaattaaaa ataaaataca aaattatatg aaggaaagaa tagaaaaaca
     6541 accattaaga gaaaattcag caggtagtgt atttaaaaat ccagagaatt tttatgcagg
     6601 tgaacttatt gaaaaatgtg gtcttaaagg atattgtatt ggagatgcct gtatttctga
     6661 aagacatgca aattttataa taaataaagg aaaagcaaaa tcatctgata tttataacct
     6721 gataaaaatt attcagaaca aagtaaaaga aaaatttaga gtagaacttg aactagaaat
     6781 aaagataata gggaaatttt gatatggaac tttttaaaaa atgtgaaaaa tttgtatcag
     6841 aaaaagggat tatagaaaat agagataaaa ttcttgtttc tgtttctgga ggtcctgatt
     6901 caatcttttt actttatttt cttttatatt tgagaaataa atataacttt tctttgtttg
     6961 tctcttatat tcaccaccat ttgagaaaac aggcagataa agaatttatg tatgtaaagg
     7021 aaatatcaaa aaaatatgga atcccgtttt attatagaaa tattaagatt gaaggaaaaa
     7081 caggaattga agaaaaaagc agagaaaaaa ggtataaggc actttatcaa attgcaaaaa
     7141 aaacaggagt taataaaata gcaacaggac atactcttga tgataatatt gaaagtgttc
     7201 ttttaaattt ttttagagga acaggacttg caggtatgtc tggaatttta ccagaaagaa
     7261 aaatttataa gaactcaaat atttttctta taagaccaat ccttactgtt agaaaaagtg
     7321 aaataaaaaa atttttaaat aacaagggaa ttaagtatta tttagataaa tcaaacttaa
     7381 acttaaaata tagaagaaat ttaataaggt ataaaataat cccatatctt aaaaaatttt
     7441 tcccatcatt tacagatatt attgcaaaaa catcttctat tttgcaggat gattttcttt
     7501 atatagaaaa agaagcaata aaaacattgg agttaatttt gaaaaatggg caatttaatg
     7561 tttcagagtt taaaaaaatt gatatttcaa tcagaagatt tatagttcaa cttttatttg
     7621 aaaaaatagc aggagtatct tataggtcat ataacaaaat tgaaaatttg agaaatttca
     7681 ttgaaggaat aaaagaaagt tatgtcccaa tttcaataat tgaagaaaga attaaagggg
     7741 aaaaaaaaga aaggaaaatt tataaagtga ttgaaatacc tggaaaatat aatgttgatg
     7801 ggatttttat tgaaagtgaa tatattaaaa aaagcccctt tatatttaaa aacatggata
     7861 aatatactgg ttattttgat ggagaaaaag ttggaaagaa aataattgta agaagtagaa
     7921 aaaaaaggga tatgtttcaa ccattaggga tgcaaaaaga aaaaaaactt tcaagatttt
     7981 taattgatag gaaaattcca atttacaaaa gggataaaat tttaattttt caatcaaaaa
     8041 atgaaataat gtgggtctgt ggagttgaaa tatctgaaaa gttcaaaatt acagaagaaa
     8101 caaaaaaaat attgaaaata aaagtaaatg tataaaattt tgctgtaact ttgtaaactt
     8161 acgaatgtat tttttctctt tactcccact ctattgtggc aggtggttta gaagttatat
     8221 cataaacaac tctattaact cctcttactt cattaacaat tcttgttgaa attctatcaa
     8281 gaactttgta agaaattttt gcccaatcag cagtcattcc atcagtgctt tttacaactc
     8341 ttattgcaat tacattttca taagttcttt tatctcccat aactccaact gtttttattg
     8401 gtagtaaaac ggcaaatgcc tgccacatag agttataaag agatgctttt ttaatttcat
     8461 cttctacaat tgtatctgcc tctcctaaaa tttcaagaag atgtttattt acactaccta
     8521 ttattctaac ggcaagacca ggtcctggga aaggatgtct atttattata aaatcaggga
     8581 tatttatctc ttttgcaatt tttcttactt catctttgaa taaaaatcta aatggttcaa
     8641 cgagagaaaa ttttagtttt tctggtagtc ctcctacatt atgatgtgtt tttatccttg
     8701 cagttggccc accaaatgcg ggaatacttt caattacatc tggatataaa gttccctgtg
     8761 ctaaaaattt tgcaccaaat tcttttgctt ctttttcaaa aatttttata aaggtatttc
     8821 caattatttt tcgttttttt tctgggtcgg caacattttt tagttgtttt aaaaaaattt
     8881 cacctgcatc aatatatttc aaattaacct ttttctgaaa ccacttaact attttttctt
     8941 tttctccctt tcttaatagc ccattatcaa caaatatgct taatgatttt tctttacaaa
     9001 catcttgaag tattaaagaa agcgttgaag aatcaactcc tccacttaat ccacatataa
     9061 ttttttcatc tcctactttt tcttttattt ctttttttat tttttcaaga attattccat
     9121 aattccatgt aggttctaat ttacatattt tgaaaagaaa attacttatt atttttttac
     9181 cttcttctgt atgagtaact tctggatgaa attgtagccc atatattttt ttatttttat
     9241 ctcttattgc cgttatggga gatatttctg ttctaccaat tattttaaaa ttatttccag
     9301 ttttttttac aatatcccaa tgactcatcc agacaattgt tttttctttt aaattattaa
     9361 aaatttcttc ttttctatca ggataaaaaa ttgttttccc atattctctt tttttacctt
     9421 cttcaacttt tccactgaaa aattcagata tgagttgcat cccataacat atcccgagaa
     9481 cataaaaact accatcaaat attctttcat caaatttata aatacctttt tgtatatgtc
     9541 ctggtccacc tgatagtata attgctttta tatctttttc ttttaatgtt tctattttta
     9601 tattaggaag gaatatctca ctataaacat tcatttctct aactcttctt gcaataagct
     9661 gggtgtattg agaaccaaaa tcaataacgc ctaccatccc tttcttcctc tgcgtttttg
     9721 tgcctctatc ataataacct tattaaaaaa aatcaaaatt tttacttata atgttgggaa
     9781 atatgttttt atttcaaaat cagttatctg ttgtctatac atatcccact caattttttt
     9841 agcagtaatt aagttattat aaatatggtc tccaagtact tcttttaaaa atttactttc
     9901 ttctgctact tcaattgctt ctattaaact acctggaaga cattttatat ttaattgctc
     9961 catttcttca tctgtcaaat gataaacatc cctttcaagc ggttcgggta attgataatt
    10021 attttcaatc cctctcattc cagcagcaga catacaagca aaagataaat atggattgca
    10081 tgcagggtca ggagaacgaa attcaaccct tgttgatttc tcttttcctg gtttgtacat
    10141 tggaattcta acaagtgaac ttctgtttct tcttgcccaa caaatataaa caggtgcttc
    10201 atacccaggg acaagtcgtt tataagaatt tacccattga ttacatacaa aagttatctc
    10261 tcttgaatga tttaatattc ctgcaatata ttttttacct atatcagaaa gattatattt
    10321 atcattttta tcaaaaaaaa catttttatc tcctttaaat aatgactgat gaacatgcat
    10381 tccacttcca ttaacaccat atattggttt tggcataaat gttgcataaa gaccattcgc
    10441 ttgtgctaca tcttttacca ccatcctgta gaccataaca atatctgcca tctctaatcc
    10501 ctctttatat cttaaatcaa tttcatgctg acttggagca ccttcatgat gtgaatattc
    10561 aacatctatt cccatttttt caagtgttaa aataacctct cttcttacat tatttcctgc
    10621 atcaagtgtt gttatatcaa aatatccacc ttcatcaagt atctgagggt tgttatcatc
    10681 tttaaaaata aaaaattcaa gttcaggacc aacattatat gaaaacccct tttcttttaa
    10741 ttttttaagt tgtttcttta aaatatatct tgaatcacct tcataagggg acctatctgg
    10801 atttaaaata tcacatatca ttgctgattc tcccctttct tgtggtctgt aaggaagaat
    10861 tgtaaaagta gacggaactg gtaaagcaat catatctgat tcatcaattc tggcaaatcc
    10921 ctttattgaa gaaccatcaa atcccatccc atcttcaaga gcaactttta actcttcatt
    10981 tgttattgaa aatcccttca tctgtccagt tatatcagca aaaaatagtc gtataaactt
    11041 aatatcattt ttttctacta tttttaaaac atcatcttta tttaaattgt tattcatagt
    11101 tcctccaaat tatttgaaaa tattttccca taattttgta gtgtatcctc caacaagccg
    11161 cactgcaatt tcatctggca attcattaac tcttctatta aatggctccg gcgtaacagg
    11221 tccatcatag ccagtttctt taattgtttt taaaaaacta gtaaggtcaa taattcctgt
    11281 ttcccctgga atatttctct cagtatctac caatttttca atatctgaaa aaggggcatc
    11341 atttatgtga acataaataa ttttattttt atctaatttt ttaatatctt ctaaacttcc
    11401 accagaagtg aaccagtgcc acgaatcaag taaaatccct gcattttcaa agtcagaaac
    11461 caaatcaagc atctgctcaa tagtatgaac aaatgggtat ttatggtctt ttctaaaaga
    11521 tggtgtccct ataaattcaa agccaatttt acacccacca attacttctg ttattttttt
    11581 tattattttt ctatgccact taaaattctc attatattcc atctcatctg aatatggttt
    11641 tatccatgtg taaattcttt ctgcctcaat ttcagatgca atcttaacaa atttttttaa
    11701 tttttctatt ccctttttaa aattttcttc tgagtaaata tcaataggca acccccatcc
    11761 accaacttta agattaaatg aagaatacat acctttgaca tagtcaactg aatatttttc
    11821 aacaagttca attgtttcat caactggtaa atccatacct tcaaattcac caatagttgc
    11881 aagtcgtagg cattctctta aacttttatt tattccaagt gcaccaggtg ccagattttt
    11941 aaacattccc acctccattt ttgtttctta ttatatcata ctttaaaatt ttttatatgt
    12001 atcttccata ttcttttgta ctttcgtaca tcgccactat attttcaaca ggagtaagag
    12061 gtgcaagatt gttcccttct ctcattataa accttttccc tttcattatt ccgctttcac
    12121 atattttttt acttcatctt taacttcatt ttctttacca tttttaagta aaacaatatt
    12181 tggtccacct ataatttcaa tttcttcacc taattcttcc cttaattttc taaaatttac
    12241 aggaaaacca gtatcaaatg aaataacatc caagttttcc tttagaaact taaataaatg
    12301 tcctgcatta ccacataagt ggatatgaac tttcccttct ttttctttaa attcttccac
    12361 taatttttta tgaaaagaaa acaaaatttt tatatttttc aactgataaa ttcgcaatta
    12421 gatcatctgc aaaagaaaaa atttttgatt taaaatttat ttctgccttt tcttcttttt
    12481 ctgctctcca ttttcttatt tctgtcattc tttttattat attttcagta acaaaactca
    12541 taaggtcaaa aaaatatttc agtaagacct ctcaattttc cagcaactgt aaatggtcca
    12601 tctgttcctt ctcatggaat tgttaaagga ccttttactt ttttcccttc aaattcaata
    12661 tctttatatc ttctattcat atattcaaaa aattccattg cttttccaag taaatttcct
    12721 tttaaagggt acggtggctc aatttcatat aatctatgtt tgttttcttt gaaaattggg
    12781 ttgacatctg gaggaataaa atttcctaaa tatataattt cacatccaaa ccaaccactt
    12841 tcataattgt tttgaaaatc aacattaatt tgccagccat cagaaggaat tctcatttct
    12901 gcatcgcata aaatattttc tttccaccac ttttgaaaaa gtaattgaca ttttatatga
    12961 atttcagggt cattataaaa ctgataaaaa gtataacctt ctttatttaa ttcaggattc
    13021 tcaaaaaaat ttcttatact tccaccaaca attacaggga ctcttccatg tctcttctct
    13081 ttgtaatcat cccatagttt tttttacttc ttcattatgt ttttcataat caattttctc
    13141 ttttaagaaa aaactcatta tccccccatt tagaattttt atttattaga tttttttata
    13201 atatggttgg caagacatat atacataaag gggtaatagt ataaagttta aatgtaaaat
    13261 taaaagaagt acatgaataa aaaaatttca aatttttgcc tctgtgtttg acctaatcca
    13321 ctttactgat atctatcatc ctcatttcta aaacagtatt tctcttctta atttaccttt
    13381 ataaattacc aaaccctctt ctattggttt atttacaaac cagttttctt ctccctttaa
    13441 tatctctttt aaatatggaa aagttgcata atttaaagca attgttgatg tttttggaac
    13501 aacaccagga atatttggaa cacaataatg gataatatcc ttataaataa aaattgggtt
    13561 tttgtgagtt gtggacttac ttgtctcaca taatccacct tcatctattg caacatcaac
    13621 aataacactc cctttttcta ttacatctaa atcttcttcc tttataataa ctggtgttct
    13681 ttttccaggt ataagagcag cacctacaat tacatctgct ttttttaaag aacttattaa
    13741 attttctcta tccgaatata agacatctgc ttttttaaat ctttctttaa gaattttaat
    13801 tttattctca gcaatctcaa gaaatgtaat ttttgcccca atcccctctg ctataatacc
    13861 agcagaatat ccaacaacgc caccaccaat tattaaaaca ttaccacttt caacattttc
    13921 tactcctgat aacaaaatcc cctttccaca atattctttt tctaaatatt tcattccttc
    13981 ctgaatactt aatttcccag caatttcact cattggtttt aaaattggca aatttccatt
    14041 ttcttctaca agttcataag caatacagtt aacttttttc tcaattaatt tctctgtcat
    14101 atttctattt gatgaaaaat ggcaaaaagt gaataatgtc tgtccctcac ttaaaagttt
    14161 tatttcttct tcctggggtt cttttacctt tacaattaaa tcacattttt tatatatatc
    14221 ttcaagtcct ttaacaattt ctgcaccaac ttctttatag tcattatctg aaaaacccgc
    14281 gcctacgcct gcattttctt gaataaacac cttatgccca tcctttataa tatctttaac
    14341 aaaagatgga attaaaccaa ctctatattc ttcaactttt atttcttttg gtacacctat
    14401 tatcattttt tcatctcctt tactgtttca attactttat cagccatata ttcaacttgt
    14461 ttttttgtta gtggatataa aggcaaatgc gtaaatctgt gtaagaataa atcttcagca
    14521 ttaggacatg acttttttat cttttctgta tcatatccca attgtttcag gtatgagaac
    14581 ctatataatg gagcaaaatg ctgtatttga gtaactcctt tttctgtaag tttttgttta
    14641 aataattgaa tatctccttt taatttttct ggttctattt gtataagata taaatgatga
    14701 gttggggtta tatcatatgt atctaatgga ggagttatta ttccatcaac atctttaaaa
    14761 caactattca aaaattcagc attttttctt ctttctttaa ttatcttttc aatcctctta
    14821 atctgactta ataatgcaac tgcttgaatt tctgttgtag aatgatttcc agcaggtata
    14881 tagtcacttt ctctttttaa tgcgactaca tcataaagcc agtgttcaat tggatttgaa
    14941 ctatcaaaac caacaaatct tgctttcgga aagtccttac caatttctga atttgtaaca
    15001 actataccac cttcgccaag agaagtaaca tttttaactt catgaaaact gaaagaacca
    15061 aagtcaccaa ttgttccaat tgcttttctt ttataccaac caccaaaagc atgtgctgca
    15121 tcttctacaa cttttaaatt atatgttttg gcaattttca ttattctagc catatcacat
    15181 ggataaccac cgatgtgaac tggtattatt gcttttgtct tttttgttat ttttctttca
    15241 atatcatccg ggtcaatatt tatggttctt ctatcaatat ctaccaatac aactttcgct
    15301 cctattaaaa gaggataaga aattgttgaa caaaaagtta ttgcaggtgc tataacttca
    15361 tcacctggtt ttagtccaga aaatttaagt cctatttcaa atccagcagt tgcatttgtt
    15421 ataaaacaag catattttac atttaaaaat ttgcagacat attcttctaa ttcaagaact
    15481 ttatttccaa gtgtaagttt agttgcatat ctcccatatt tgagaagatt tttaagctcc
    15541 tttttcacat tttttaactg ttttttataa tttttattat caactggctg gattaaaaat
    15601 tttaaaattt cttcaatatc atcaatattt gtactttctc caactgctgc ccatggaaca
    15661 gcaacatttc ctgtatcata cctgaaatca cttactttct tcttattcat atctctctcc
    15721 ttttcctgtt tttcttactg ccaaaattgg ttaattaaat tcactctacc ttttctcaca
    15781 aagacaaatg aaatgtgaaa agtaagatgt gagaagtaga aatcccgtaa gcgcaactta
    15841 tcacttctgc gcctgcccgt ctgagcatta gcgaaggagg gtagggatgg tctgtaagta
    15901 agtcctaaac ccttctatgg aagagaatcc accccctcgg gcgtagccca gagaaattgt
    15961 gtggacacta cgggcgagac cacattcctc tctacaaaga tagaggggta aaccttgaaa
    16021 gggggagatt ttaaaatcca ttctaacctt cctttgataa aggaagacac tctatatcaa
    16081 attaaaaagt aaaaagtttt ttaacaagtt aaaaactagg agaaatttct ccttgaaatt
    16141 gttctttgac aattttaaga tgcttttgtt catcataagt tgacctgttt ttcttacagg
    16201 aacatttttg tctttttttt attatagaat accatttatt tttgt
//
LOCUS       DAOOVW010000037        15526 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_2547_length_15526_cov_7.749208, whole genome shotgun sequence.
ACCESSION   DAOOVW010000037 DAOOVW010000000
VERSION     DAOOVW010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15526
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2547_length_15526_cov_7.749208"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..1239
                     /locus_tag="PLW95_05925"
     CDS             160..1239
                     /locus_tag="PLW95_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOV22201.1"
                     /translation="MSYKGKYKIGEILVYAVEKKASDIHIVAKFPANIRVNGQLIVTE
                     FPELSSDECKDLIYDILSPSQIKSFEEKKELDFSYGVSGLGRFRINLHYQRGTIGIAI
                     RPIKDKIPTFAELGLPSDMLKKLLSQPHGLVLICGPTGSGKSTTLASMVEYLNQNFPM
                     HIITIEDPIEYIFKHKRSIVEQREIGIDTDSFNEALKRVLRQDPNVVLIGEMRDIETI
                     EAALRIAETGHLVLATLHTGEVIQAMGRIVNSFSENLQQTVKTQLSLVLLGVFVQQLL
                     PKKDNNGRILATEIMITNSAIRNLIREGHFEQIYSQIQLGGESGMRTMNSSLLSLYKN
                     GLISKDIALANSPNVKELKKLLGGT"
     gene            1591..2808
                     /locus_tag="PLW95_05930"
     CDS             1591..2808
                     /locus_tag="PLW95_05930"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOV22202.1"
                     /translation="MAKYFYVARDFSGKRREGVIDADSNMTVILNLRKQNLIPIEINF
                     IGEKGLSPIVSQKKQTRGRVSLGELAIFSRQFTTMLDAGVPIIDIIGDLSEQTTNKYF
                     SYVLSKIKIEIQEGSNFSNALTKFPNVFSPLYVSLVRTGEESGNLTKIMEELAKYLED
                     QIALLRKVRQALSYPSVIFVFFLGVISFVFLFLIPKFQSIFTSLGTKLPPLTMLIFNI
                     SHAFFKFFPVIIVGIILLIIAFLIFRKTTTGRQIIDTIKLRFPFFGKLITKITLSRFS
                     RSLSTLLSGGVSIVVALEIASSGENVIVEKIIKNIKKEIISGKTLGEEMKKYKIFPPL
                     LVRMIKIGEETGRLEDMLNRTAKFFSDEVDATINIMTSIIEPVLIIGLGVVVGIVVLA
                     IYLPIFQIAGAVK"
     gene            2941..3351
                     /locus_tag="PLW95_05935"
     CDS             2941..3351
                     /locus_tag="PLW95_05935"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOV22203.1"
                     /translation="MKKIRKMMKNKKGFTLIELMVVVIIVGILASVAVPLYTAHVKQA
                     KASEGAALVGSVRTAELVYYAQHNTYTDDKTELGIDASANKYFTDYSFDGDVTATAFK
                     AITTCDDDANLKVRIDQNGYLEWTKDGGTNWNPW"
     gene            3549..3746
                     /locus_tag="PLW95_05940"
     CDS             3549..3746
                     /locus_tag="PLW95_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoisovalerate dehydrogenase"
                     /protein_id="HOV22204.1"
                     /translation="MIFLVEEPPEGGYEARALGYSIFTQADTLDKLKKEVRDAVKCHF
                     EEEEMPLIIRLHIVKEEVVKV"
     gene            3743..3998
                     /locus_tag="PLW95_05945"
                     /pseudo
     CDS             3743..3998
                     /locus_tag="PLW95_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019988388.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            4207..5211
                     /gene="pilM"
                     /locus_tag="PLW95_05950"
     CDS             4207..5211
                     /gene="pilM"
                     /locus_tag="PLW95_05950"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HOV22205.1"
                     /translation="MVPKVAVDIGEKYIKVLEGYRKKDVFLIKNFGMIENPVENWKKS
                     TEEDSSGILSNVLKVYLKELNIKDRNTVCSISGEGLVIHYFEIPEMPEEEIKNAVEIE
                     VLQVIPTGIENIEYDYSVFNVGGKKYVFFVGYPKEKCDFILNVFQKSGLKPLIMDIDG
                     LALVNCYNYVKKNNWQLPVFILNLGHNTTNLSIAQKDGFIFVRDIPFGGNNVINFISE
                     TKEISIEESEKFITDEENYEEVKNAVKNSSEELLREIEIGVEYFRTRTGERPSEFLIT
                     GGSSTLSGLKQLLEEELQENIEIWNPFEYIDENLVPPDIKERGRFFSISLGLLTRKIG
                     "
     gene            5208..5789
                     /locus_tag="PLW95_05955"
     CDS             5208..5789
                     /locus_tag="PLW95_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22206.1"
                     /translation="MIEINILRKRLERFHRRRVLTKIIIFYFGGLFLILLILGGVYLS
                     NKLIILQDKENMDKLRADIRKDELLVEMVKKFHDECNVLCKNISFYKDEINTRVVWYD
                     KLSLISNCLPPGMYLNRISFKESGSQEKKKVIIIDGYVSPIVENPRKSLSYFVQNLKR
                     DGGSFIDKITLTQINNVKTEEEVVYFVFECSLK"
     gene            6090..6629
                     /locus_tag="PLW95_05960"
     CDS             6090..6629
                     /locus_tag="PLW95_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22207.1"
                     /translation="MKEYIIYIKKYAVWMLISYAAIILVFYVFFLLPQRRFINEVKVE
                     KETVEYYYLKIKNIPVVFDTINKTLSVVKDQIKCFEWLANTDDPNFLMFQYLGEKAEK
                     YNLELSILERNLKDEKSSKYYIWKTKLVGSFPDLLKFLYEVETGDKYLKIDEIEVGKE
                     GEKVFFDLKIVGIKGGKNE"
     gene            6622..>7122
                     /locus_tag="PLW95_05965"
     CDS             6622..>7122
                     /locus_tag="PLW95_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22208.1"
                     /translation="MNEEKKRILKIGILVILLFVIFFSTLTLKGKENASAEGQKNKEK
                     RLSVVLQEAEEHFAEIKKKLQDIDNMKKTPELNLVRKDPFNPIKRKIDVEKVEKIIRE
                     EEKKEITFLISGIVYDEQKPMVIIDDNVKCEGEYIEEYQIYKIFPDSILLKNGNDFYE
                     LKMNFEE"
     assembly_gap    7123..7222
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7235..8545
                     /locus_tag="PLW95_05970"
     CDS             7235..8545
                     /locus_tag="PLW95_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOV22209.1"
                     /translation="MKKQVKIFVFIFFLPLILFANSEFTFHNAPLNLVLQALSKKTGI
                     KLITNAELGEKPISVYLENVSGEQAIDSILKANGLYREKLPDTEIYVVKEFKETPLLD
                     CETFFLQYAKAEELGKVLTSFIGPNGKISVDTRTNSLVIKDTSENLEELKKIIKSLDK
                     VIPEISIEAVLVELTNEGLKDLGIKWNMEGSFVGGAKDVPYPWSKSITREIVNPRDVG
                     GEGGTTGGTETTAGGQFILGTVSFQTLTASLRLLESQGEGNILANPRITTLNDSPATI
                     KITRNMAVAERTVYNTETGAPSTKEPIYAEIGVMLIATPHINEEGFITLEVEPTVSSA
                     EKSPFFADAVDTHNRTAKTTVMVKDGETIVIGGLLRTDTTKSNTKVPLLGDIFPFLFK
                     NKADQTKKTDLVIFLTPRIITPSKASSIAEEEKNRIQGAMKDEK"
     gene            8535..8888
                     /locus_tag="PLW95_05975"
     CDS             8535..8888
                     /locus_tag="PLW95_05975"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOV22210.1"
                     /translation="MKNKGFTIIEVIISLIILIIVIIGSSVFFYNSRKNLVNANLERL
                     GTWKAIEKMELLKSGSYSNIINQTENISLGGTPAQIITSVQNVDENGTTFKLVKVEVN
                     WGNNSVSLTTYIAEK"
     gene            9167..9634
                     /locus_tag="PLW95_05980"
     CDS             9167..9634
                     /locus_tag="PLW95_05980"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOV22211.1"
                     /translation="MKKKGITLIELIVVIVIGAIGLLAVSFFAMQSWKEWNENEQIKT
                     LQEEMDLVSYTIKGVLEEASNIEILDNGPLSGTKINASYKNEWQKEFYPSGNELIVED
                     IKNETTRTVTNYLKSISFQQDNTSSDTVLVNLKVGKGGKELENSFIIFLRNKS"
     gene            9925..10338
                     /locus_tag="PLW95_05985"
     CDS             9925..10338
                     /locus_tag="PLW95_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HOV22212.1"
                     /translation="MKRKYIGRCRQLRKNQTDAEKKLWMLLRGRQIEGVKFRRQFPID
                     KYIIDFYAPEYKLGIEADGGQHYYGKTKQKDKIREIELSRLGVKILRFSDTDILKNIE
                     GVWEVIQTELLKKKPPSPFPSPRWVEGKRGESRKE"
     gene            10691..11929
                     /locus_tag="PLW95_05990"
     CDS             10691..11929
                     /locus_tag="PLW95_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22213.1"
                     /translation="MNRKKGYLLPLVLILILISLIVGFTTLYLSELGRTETIRRLYRE
                     KAFYIAEGGAQRSYAHLKKDISWVPESSSQSLGGGTFYVIKDSTSSETIIITSTGTFN
                     NVEEKVELTIKKSGGGIFSKGVFGSSQVYLDGSSQIWGYDSRTGATSENADVGSNTLV
                     NVNWNATLYGNAAVPPNGSITFPEQKPNAITGQKKKDAETRTLPPVTIPSDLQNLSYT
                     QYGGTGLSGNYQLSNGNYTINDWPYAATISSGNYHFRSIQLGKGNPQLNLSGKIRMYI
                     EETLSSSFGGDIVLSAGTTVEIYMGRTNGKITFDSDSQINYNNSPANLAIYVASSKAV
                     DLLSNVRINAVIYAPNSVCNITGSSELRGGIVANQLNLTGNVRLYDDIALRQVSVPGD
                     TSGGTGGIQIVKWRKPNWKQ"
     gene            complement(11973..12557)
                     /locus_tag="PLW95_05995"
     CDS             complement(11973..12557)
                     /locus_tag="PLW95_05995"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOV22214.1"
                     /translation="MIKNLSAAFLSIFISMNVLGILPIYISFVQNVQTKEIRKIVNTS
                     ILFTFLITISFIFIGKFIFYIIGIETFDFLIAGGLVLIVLAILMLLDIAYKQSFETKS
                     FAIVPLATPLLAGPGLLTTSITVAEIYGYPIVCYALIFNLFIAYIILRYSSYILDFLG
                     KEGIKAISKIASLLLASLGVMMIRTGLISLMKTL"
     gene            complement(12597..13586)
                     /gene="mltG"
                     /locus_tag="PLW95_06000"
     CDS             complement(12597..13586)
                     /gene="mltG"
                     /locus_tag="PLW95_06000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOV22215.1"
                     /translation="MKKKIIFIIIAFLIILLGIIKIMTLPPLVKPPKLVEIPENLNAS
                     QIAKILYQKKVIKNPSLFLFLTNRLKVQNKLKSGIYEFSGRTPLKKVVEKLVKGEVKL
                     VKVTIPEGKNIVEIADILEKKGLIKNKDDFIKHAKEKKLEGFLFPDTYFFPLKISIEG
                     ITYTMWKRFNDVWYDLKGERINDENFNEVKKIVTIASIVEKEATYPEGKRIVAGIIYK
                     RLKKGLPIQSCATVEYAIGQHKQKLNKKDLKVKSPYNTYIHKGLPPTPICNPGKVSLQ
                     AALNPKKTDYLFFVSMGNGKNYFSKTYEEHLRAKEIYITSRQEKVENETFIFP"
     gene            complement(13583..14824)
                     /locus_tag="PLW95_06005"
     CDS             complement(13583..14824)
                     /locus_tag="PLW95_06005"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HOV22216.1"
                     /translation="MEKKYPKIEIINVGSELLLHKLNTDILLISDILLKSGLRVSKCI
                     IVGDKKDEIKKAIENSLVESEILIITGGLGPTFDDLTREAISDVLNRKLIFSNYIWEK
                     IKERFQKIGRDLPEINKKQSYIIDGCEIIENEVGTAPGMFIEENNKIIIVLPGPPNEL
                     GPMLENTIEKIKNKFSSIENVKIAKFSVCGVPESVVEEKMRDIIEREKSEHIILAHPD
                     LIELLFIFDLSIYQEKTEEIDKIFKERFGENYLGINPPALPEIIGKILKEKKLKLAIA
                     ESCSGGLACKLITDIPGSSEYFTGGFVTYSNTLKKRILRVPKCALKKYGAVSEEVAIC
                     MAKGAKKYGKSDISLSFTGIAGPTGGSKEKPVGLVWIGVGLPKNKNFAQKFIFNGNRA
                     TIRERAVRTGLNILRKEIMKL"
     gene            complement(14829..15377)
                     /gene="yfcE"
                     /locus_tag="PLW95_06010"
     CDS             complement(14829..15377)
                     /gene="yfcE"
                     /locus_tag="PLW95_06010"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006988.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HOV22217.1"
                     /translation="MRKIGIISDTHGSLERWEMAWEILKDTDFIIHCGDIFNHGPGNP
                     IPEGYCPKKLLEKLNNLQKPIFVVKGNCDSEVDQTFLLVPFVSPYFLGIIENYRIIAT
                     HGHLFNDNFFETVKKWNINFFISGHTHLWKIEKKENIVILNPGSPSLPKNEPSCGLID
                     FQNRKVSIFDIIKKTILKEEKF"
BASE COUNT         5658 a         2124 c         2745 g         4899 t
ORIGIN      
        1 aatccatccc atccttccct tggaaaggga aggagtatct tccccctttt agaaaggggg
       61 gatgaagggg gggatttcat ccttctcccc gttggggaga aggtagggat gagggggatc
      121 gctactaaca tataaaaaag attaataaat atattaaaga tgagttacaa aggcaaatat
      181 aaaataggag aaattcttgt ttatgcagtt gagaaaaaag catcggatat tcatattgtt
      241 gcaaaatttc ctgccaatat aagagtcaat ggtcaattaa ttgttactga atttcctgaa
      301 ctatcttctg atgaatgtaa ggacttaatt tatgatattt taagtccctc tcaaattaaa
      361 tcatttgaag aaaaaaaaga attagatttt tcttatggag taagtggatt gggaagattt
      421 agaatcaact tacattatca aaggggcaca ataggaatag caataagacc aattaaagac
      481 aaaattccaa catttgctga actgggatta ccttctgata tgctaaaaaa attactttct
      541 caaccgcatg ggttagttct aatatgtggc ccaactggaa gtggaaagtc cactactctt
      601 gcttcaatgg ttgaatatct taatcaaaat tttccaatgc atattattac aatagaagac
      661 cctattgagt atattttcaa acacaaaagg tcaatagttg aacagcggga aattggaata
      721 gatactgact catttaatga agcattaaaa agagttttaa ggcaggaccc aaatgttgtt
      781 ttaattgggg aaatgagaga tattgaaaca attgaagcag cactaagaat tgctgaaaca
      841 gggcatcttg ttcttgcgac tttacataca ggtgaagtaa ttcaagcaat gggaagaatt
      901 gtaaatagtt tttcagaaaa tttacaacag acagttaaaa cacaactttc acttgttctt
      961 cttggtgtct ttgttcaaca attgttacct aaaaaagata ataacggtag aattcttgca
     1021 acagaaataa tgataaccaa ttctgcaata aggaatttaa tcagagaagg acactttgaa
     1081 caaatttatt ctcaaattca attaggtgga gaaagtggaa tgagaacaat gaattcttct
     1141 ttgctttctc tttacaaaaa cggacttatt tcaaaagaca ttgcattagc aaattctccc
     1201 aatgtcaaag aactaaaaaa acttttagga ggcacttaat actcagggca acttagttga
     1261 ctgagcagta taaagtagag agtagtaagt agcgagatta aaaaagcccc cctcaccttg
     1321 aacctctccc caacggggag aggcagaaaa gaaaaaaagg ggagagggga aaaataaaga
     1381 attttaattt tcactataat tttcttcgcc ccacagggga gaagattaag atgaggggga
     1441 attgttctac acaggcagtt taaaattaag ttgcacgaac cagccctacc ctccttctct
     1501 aatgctcaga cgggcaagcg cagaagcgat aagttgagca gacggagttt taaagtgcaa
     1561 agtgtaaagt aaaaaaaata ggaattaaaa atggcgaaat atttttatgt agcgagagat
     1621 ttttcaggta aaaggagaga aggagtaata gatgcggatt caaatatgac tgtaattctt
     1681 aatttgagga aacaaaatct tatccctatt gaaattaact ttattggaga aaaaggactt
     1741 tccccaatag tttctcaaaa aaaacaaaca agaggaagag tttcacttgg tgaacttgca
     1801 atattttcaa gacaatttac tacaatgctt gatgcaggtg tgcctattat tgatataatt
     1861 ggtgaccttt ctgaacaaac aaccaacaaa tatttttcct atgttctttc aaaaataaag
     1921 attgaaattc aagaaggaag caatttttca aatgctttga caaaatttcc aaatgttttt
     1981 tctcctcttt atgtttctct tgttagaaca ggtgaagaaa gtggaaattt aacaaaaatt
     2041 atggaagaac ttgcaaaata tcttgaggac caaattgcac tattaagaaa agtaagacag
     2101 gcgttaagtt atccttctgt gatatttgtt tttttcttag gggttatttc ttttgttttt
     2161 ttatttttaa tcccaaaatt tcagtcaatt tttaccagtt taggaactaa acttcctccc
     2221 cttacaatgt taattttcaa tataagccat gcttttttca aattttttcc agtaattatt
     2281 gttggaataa ttttacttat aattgccttt ttaattttta gaaaaaccac cacaggaagg
     2341 caaattatag atacaattaa attaagattt cctttttttg gaaaacttat tacaaaaatt
     2401 actctttcaa gattttctcg ttcactttct acattacttt caggaggtgt aagtatagta
     2461 gttgctttag aaattgcatc atctggagaa aatgttatag ttgaaaaaat tataaaaaat
     2521 ataaaaaaag aaataatatc gggaaaaacg cttggtgaag aaatgaaaaa atacaaaatt
     2581 tttccaccac ttcttgtgag aatgataaaa ataggagaag aaacaggtcg acttgaagat
     2641 atgcttaata gaactgcaaa attttttagt gatgaagttg atgcaacaat aaacataatg
     2701 acttcaataa tagagccagt gcttataatt ggattagggg ttgttgttgg aatagttgtt
     2761 ttagcaatat atcttccaat ttttcaaatt gcaggagcag taaagtaaaa attcactcag
     2821 gcactggttc cgaaaagaaa tttaaccccc gcggttatac tttgcgggga atttttaaaa
     2881 aatttgacaa aaatattgac aataaggtat aatataacaa aaaaaaggag gtgagaaagg
     2941 atgaagaaaa taagaaaaat gatgaagaac aaaaaggggt tcacactaat agaattgatg
     3001 gttgtagtta tcatcgttgg tatattagcg tcagtagcag ttcctcttta cactgctcat
     3061 gttaagcagg caaaagcatc agaaggcgca gcgcttgttg gttccgtcag aactgcagag
     3121 ttagtttatt atgcacaaca taatacatat acagatgaca aaactgagtt aggaatagat
     3181 gcatctgcaa ataaatactt cactgattac agctttgacg gtgatgtgac tgcaacagct
     3241 tttaaagcaa taacaacctg tgatgatgat gcaaatttaa aagtcagaat agaccagaat
     3301 gggtatttag aatggactaa ggatggtgga actaactgga acccttggta aaaattagaa
     3361 aaaggggcgg acttaaaagt ccgccccttt cttttcttca ttgttttttt cctctttgta
     3421 aaaattttca aaaaattttc tttaattttt ccccttataa ctcactcaaa ataacaccat
     3481 atttttaaaa aatatgataa aatatatata aaaagtaaaa tgtagagaga acaaaaagag
     3541 ataaagaaat tatattttta gtagaagaac ccccagaagg gggatatgaa gcaagagcat
     3601 taggatattc tatttttaca caggcagata cattggataa attaaagaaa gaggtcaggg
     3661 atgctgttaa atgtcatttt gaagaagagg agatgcctct tattatcagg ttgcatatag
     3721 taaaagagga agtggtaaaa gtatgaaaat tccaagagat ataaatggag aagaattagt
     3781 aaaattttta aaaaaatatg gatatgaaat tacaagacag aacaggaagt catatcagat
     3841 taacaactca caaaaatgga acgcaccata ttacaattcc taaacataaa gttttgagag
     3901 taggaacttt aaatttcatc ttaaaagatg ttgctgaaca cctaaaattt gaaaaagaac
     3961 ttctaattaa agaaataaaa ttatgccgta gaagttgaac agaattatgt taataaatag
     4021 tagtgtaaaa aaataaaaag aagcaaagcc cccctcaccc tcgcccctct cccctatggg
     4081 gagaggagaa aataaatgaa aaaagaaaaa ggttccttcc ttatttcttc tccccttggg
     4141 ggagaagata gatgaggggg gattgcttgc tactaaaata taaatgagga ttggataaaa
     4201 ttaaaaatgg tacctaaagt tgcagtagat attggagaaa agtatattaa agtgcttgaa
     4261 ggatatagaa aaaaagatgt ttttcttata aaaaattttg gtatgataga aaatccagtt
     4321 gaaaattgga agaaaagtac tgaggaggat tcatctggaa ttttatctaa tgttttaaaa
     4381 gtatatctca aagaacttaa tataaaagat agaaatactg tatgttctat cagtggtgaa
     4441 ggtttagtta tacattattt tgaaatacca gaaatgccag aagaagaaat aaaaaatgca
     4501 gttgagattg aagttcttca ggtaattcca acaggaattg aaaatataga atatgattat
     4561 tctgtattca atgttggagg gaaaaaatat gttttttttg ttgggtatcc aaaagaaaaa
     4621 tgtgatttca ttttaaatgt ttttcaaaaa tcaggactta aaccattaat tatggatata
     4681 gatggtcttg ctcttgttaa ttgttataat tatgtgaaaa agaataattg gcaattacct
     4741 gtttttattc ttaatttggg gcataacaca acaaaccttt caattgctca aaaggatgga
     4801 tttatttttg ttagagatat tccttttggt gggaataatg taataaattt tatatctgaa
     4861 acaaaagaaa tttcaattga agaaagtgaa aaatttatta cagatgagga gaattatgaa
     4921 gaagtaaaaa atgctgtaaa aaactcctct gaagaacttt tgagagaaat tgaaataggg
     4981 gtggagtatt ttagaacaag aacaggagaa agaccatctg aatttcttat aacaggtgga
     5041 agttctactc tttctggttt aaaacaattg ttagaagaag aattacagga aaatattgaa
     5101 atatggaatc catttgaata catagatgaa aatttagttc ctcctgatat aaaagaacgg
     5161 ggtaggtttt tttctatatc attagggctt cttacaagga aaattggatg atagaaataa
     5221 atattctgcg taaaagattg gaaagatttc atagaagaag ggtattaaca aaaattatta
     5281 tattttattt tggaggtctt tttctaattc ttttaattct tggtggagta tacctatcaa
     5341 ataaacttat aattttacag gataaagaaa atatggataa attaagggca gatataagaa
     5401 aagatgaatt attagtagaa atggtaaaaa aatttcatga tgaatgtaat gttttatgta
     5461 aaaatatatc tttttataaa gatgagataa ataccagagt tgtatggtat gataaactat
     5521 ctttaatttc caattgtcta cctcctggga tgtatcttaa tagaatttct tttaaagaaa
     5581 gtggaagtca agaaaaaaag aaagtgatta ttattgatgg atatgtgtct ccaattgttg
     5641 aaaacccaag aaaatcactt tcttattttg ttcaaaatct taaaagagat ggaggtagtt
     5701 ttatagataa aataacccta acacaaataa ataatgtaaa aacggaagaa gaagttgttt
     5761 attttgtgtt tgaatgttct ttaaaataat tgttaattcc ctctccccgc aggggagagg
     5821 gaagaaaggg gagaggcaga aagagaagga gaaaaagttt cttctccccg ttggggagaa
     5881 gttagatgag gggggttgat attttccact ttgacaacgg gagattttgg ggaattacag
     5941 gaaatccatc cctgccttcc tttttcaaag gaaggagaca atctttcccc ctttagtaaa
     6001 gggggattga gggggatttg aagggggatt gaaattttgt gcctgagtat aacacaactt
     6061 ctactgatat ttacgaataa aaaatgaaaa tgaaagaata tataatttat attaaaaaat
     6121 atgctgtttg gatgctgatt tcttatgcag caattatatt ggttttttat gttttctttt
     6181 tattgccaca aaggagattt ataaatgagg taaaggttga aaaagaaact gttgaatatt
     6241 actatttaaa aattaaaaat atacctgtgg tttttgatac aataaacaaa actctttctg
     6301 ttgtaaaaga tcagataaaa tgttttgaat ggcttgcaaa tactgatgac ccgaattttc
     6361 ttatgtttca atatcttgga gaaaaggcag aaaaatataa tcttgaacta tctattcttg
     6421 aaagaaattt aaaagatgaa aaaagttcta aatattatat atggaaaaca aaacttgttg
     6481 gttcttttcc agacctttta aaatttcttt atgaggttga aacaggagat aaatatctaa
     6541 aaatagatga gattgaagtt gggaaagaag gggaaaaagt attttttgat ttaaaaattg
     6601 ttggaataaa agggggaaaa aatgaatgaa gagaaaaaga gaattttaaa aattggaata
     6661 ttggttattc ttctatttgt tatatttttt tcaactttaa ctttaaaagg aaaagaaaat
     6721 gcaagtgcag aaggacaaaa aaataaagag aaaagattaa gtgttgtctt acaagaagca
     6781 gaagaacatt ttgcagaaat taaaaagaaa ctacaggata ttgacaatat gaaaaaaaca
     6841 cctgaattga atcttgtaag aaaagaccca tttaatccaa taaaaagaaa aattgatgtt
     6901 gaaaaagtag agaaaattat cagagaagaa gaaaaaaaag aaattacctt tttaatttca
     6961 gggattgtat atgatgaaca aaaaccaatg gtgataatag atgataatgt aaaatgtgaa
     7021 ggggaatata tagaggaata tcaaatatat aaaatttttc ctgatagtat tttattaaaa
     7081 aatggaaacg atttttatga actaaaaatg aattttgaag aannnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnaagaggtg aaaaatgaaa aaacaggtta agatatttgt
     7261 ttttattttt tttcttccat tgattctttt tgctaattct gaatttactt tccataatgc
     7321 accattaaat ctggtgttac aggcacttag taaaaaaaca ggtataaaac taatcacaaa
     7381 cgcagaactt ggagaaaaac caataagtgt atatttagaa aatgtttctg gagagcaggc
     7441 aattgattca attttaaaag caaatggcct ttatagagaa aaattacctg atacagaaat
     7501 atatgttgta aaagaattta aagaaacacc tttattggat tgtgagacat tttttcttca
     7561 atatgcaaaa gcagaggaat tagggaaagt tcttacttca tttataggac caaatgggaa
     7621 aataagtgtt gatacaagaa caaatagttt agtaataaaa gatacatctg aaaatttaga
     7681 agaactgaaa aaaataataa agtcattaga taaagttatt cctgaaattt caattgaagc
     7741 agttcttgta gaacttacaa atgagggctt aaaggattta ggaataaaat ggaatatgga
     7801 gggttcattt gtaggaggag caaaagatgt tccttacccg tggagcaaaa gcataacaag
     7861 agaaattgta aatccaagag atgtaggggg agaaggtggg acaacaggtg gcacagaaac
     7921 tactgctggt ggacaattta ttcttgggac agtctcgttt cagacactta ctgcaagttt
     7981 aagattactt gaaagtcagg gagaaggaaa tatattagca aatccaagaa ttacaacttt
     8041 aaatgattca cccgcaacaa taaaaataac aagaaatatg gctgttgctg aaaggacagt
     8101 ttataataca gaaacagggg ccccatctac aaaagagcca atttatgcag aaattggtgt
     8161 tatgcttatt gcaactcctc atataaatga ggaaggattt ataactcttg aagttgaacc
     8221 aactgttagt tctgctgaaa aatcgccctt ttttgctgat gcagttgata ctcacaatag
     8281 aactgcaaaa acaactgtta tggtaaaaga tggtgaaaca atagtaattg gtggactttt
     8341 aaggacagat acaacaaaaa gtaatacaaa agttccttta ttaggagata tatttccatt
     8401 tctcttcaaa aataaagcag accagacaaa aaaaacagac cttgttatat ttttaacacc
     8461 aagaataatt actccttcaa aagcaagttc aattgctgaa gaagaaaaaa atagaattca
     8521 gggtgcaatg aaagatgaaa aataaaggtt ttaccataat agaagtcatt atttctctta
     8581 taatacttat aatagtcata attggtagtt ctgtattttt ttataatagt agaaagaatt
     8641 tagttaacgc aaatttggaa agattgggaa cctggaaagc aattgaaaaa atggaacttc
     8701 ttaaaagtgg tagttacagt aatataatca accagacgga aaatatttct cttggtggaa
     8761 ctcctgctca aataattacc agtgtccaga atgtagatga aaatgggaca actttcaaat
     8821 tggtaaaagt ggaagtgaac tggggaaata atagtgtttc tttaacaaca tatattgcag
     8881 aaaaataata tttccaatag ttctcttctc tccagcggag agaaggaaag aaaggggaga
     8941 gtagaaagga ataaaaagaa gaaaaatccc ccctcacctc tatccctctg ggcgtagccc
     9001 aacgggcgag cctctccctc cgccacaaag attggcggac aggctaaggg gagaggagag
     9061 aaagaaaaaa ggggagatag gataagtaaa gaattttaat tttcactata attttcttct
     9121 cctcacaggg gagaagatta agatgagggg ggataagaga ggataaatga aaaagaaggg
     9181 aataactctt attgaattga tagttgtaat agtaatagga gcaattggac ttttagcagt
     9241 tagttttttt gctatgcaga gttggaaaga atggaatgaa aatgaacaaa taaaaacatt
     9301 acaggaagaa atggatttag ttagttatac aataaaaggg gtattggaag aagcatcaaa
     9361 tatagaaatt cttgataatg gaccactttc aggaacaaaa ataaatgcct cttataaaaa
     9421 tgaatggcaa aaagaatttt atccttcggg aaatgaactt attgttgaag atattaaaaa
     9481 tgaaacaaca aggacagtga caaattattt aaaaagtatt tcttttcaac aggataatac
     9541 aagttcagat actgttcttg tgaatttaaa agttggaaaa ggtggaaagg aacttgaaaa
     9601 ttcttttata atttttttaa gaaacaaaag ttagtatccc cctcaccccc ttttgacaaa
     9661 ggaatgagta ttttaagaca ttctcctctt tataaaaccg actgaagttg ccaagattag
     9721 caaagcagag ttggtgcgtc gttcaaaaat gctcccctct tttataaaga ggggtggggg
     9781 gagattttat aaaatccatc ccaaccttcc ttttcaaaag gaaggagaaa acaattcccc
     9841 tcttttataa agagggggtg gggggagatt ttgacttttc ttttcttctc ccctttgggg
     9901 agaagattaa gatgaggggg ataagtgaaa agaaaataca tagggagatg tagacaatta
     9961 aggaaaaatc aaacagatgc agaaaagaaa ttatggatgt tgttaagggg acgacaaata
    10021 gaaggagtaa aatttaggag acaatttcct attgataaat acataataga tttctatgct
    10081 cctgaatata aattggggat agaagcagat ggtggacaac attactacgg taaaactaaa
    10141 caaaaagata aaattagaga aattgaacta tctcgtttag gagtaaagat attaagattc
    10201 agcgatacag atattttaaa aaatattgag ggtgtgtggg aagtaattca aacagaatta
    10261 ctgaagaaaa agcccccctc acccttcccc tctccccggt gggtagaggg aaaaagaggg
    10321 gagagtagaa aggaataaaa agaaacaaaa gcccccacac ctctatccct ctgggcgtag
    10381 cccaacgggc gagcctctcc cctatgggga gaggtagaaa gagaggggtg taatttcttc
    10441 tccccaaagg ggagaatatt atgctcgccc gtagggtcct cgctttttgc tacggatccc
    10501 tctgggcata gcccaacggg cgagcctgtg gactatgccc gaaggatgag gggaagggaa
    10561 aagcaaaaag cccccctcac ccttgccctc tccccgcagg ggagagggaa taaagaaaag
    10621 aagggagagg ggaaagaaaa aaggggggag aggcagaaag agaggggata gagattttag
    10681 gagggagaaa atgaatagaa aaaaagggta tcttttgcca ttagttctta ttttaatact
    10741 tatatcgctt attgttggtt ttactactct ttatttaagt gaattaggta gaacagaaac
    10801 tataagaaga ttatatagag aaaaggcatt ttacattgca gaaggtggtg ctcaaaggtc
    10861 gtatgctcat ttgaaaaaag atatttcttg ggttcctgaa agtagttcac aatcattagg
    10921 aggaggaaca ttttatgtaa taaaagatag tacaagtagt gaaacaataa taattacttc
    10981 aactggaaca tttaataatg ttgaagaaaa agtagaacta acaataaaaa agtcaggtgg
    11041 tggtattttc agtaagggag tatttggtag ttcacaagtt tatttagatg gtagttctca
    11101 aatatgggga tatgattcaa ggactggagc aacaagtgaa aatgcagatg taggaagtaa
    11161 tacacttgta aatgttaact ggaatgcaac actatatgga aatgctgctg tgccacctaa
    11221 tggtagtata acatttcctg aacagaaacc taatgcaatt acgggacaaa aaaagaaaga
    11281 tgcagaaaca agaactcttc cgcctgtgac aattccaagt gatttacaga atctttcata
    11341 tactcaatat ggaggaacag gattaagtgg taattaccaa ctttctaatg gtaattatac
    11401 tataaatgac tggccttatg cagccactat atcttctgga aattatcatt ttcgctctat
    11461 ccaacttgga aaaggaaatc cacaacttaa cttatcaggg aaaattagaa tgtatataga
    11521 agaaacatta tcttcttcat ttggaggaga tatagtttta tcagcaggaa ctactgttga
    11581 aatttatatg ggaaggacta atggaaaaat tacatttgat agtgatagtc aaataaatta
    11641 taataatagt cctgcaaatc ttgcgatata tgttgcctct tccaaagcag tagaccttct
    11701 cagtaatgtc agaattaacg ctgtaattta tgctcctaac tcggtatgta acatcactgg
    11761 tagttcagaa ttaagaggtg gaattgttgc taatcaatta aatcttacag ggaatgtaag
    11821 gttatatgat gatattgctt taaggcaagt aagtgttcct ggagatactt ctggtggaac
    11881 tggtggaata caaattgtta aatggcgaaa acccaactgg aaacaataat tttttcccca
    11941 caccaggtct gccacaccag gtctggagaa atttatagtg ttttcattag tgatataagg
    12001 cctgttctaa tcatcataac tcctaaactt gcaagaagta aacttgcaat tttagatatc
    12061 gcttttattc cctctttccc taaaaaatca agtatatatg aagaatatct caatataata
    12121 taggcaataa aaagattaaa aataagtgca taacagacaa ttggataacc ataaatttct
    12181 gcaactgtaa tagaagtagt aagcaatcca ggacctgcca ataaaggagt tgcaagtgga
    12241 actatggcaa atgattttgt ctcaaaagat tgtttataag caatatcaag aagcatcaat
    12301 atcgcaagaa caataagaac taaaccacca gcaataagaa aatcaaatgt ttctattcca
    12361 attatgtaaa aaatgaattt cccaataaaa ataaaagaaa ttgtaattaa aaatgtgaaa
    12421 agaattgagg tattaacaat ttttcttatt tcttttgtct ggacattttg aacaaaagaa
    12481 atataaatag gtaaaattcc caaaacattc attgaaataa atatagacag aaaagcagca
    12541 gataaatttt ttatcatttt atttttctcc tttttatttc tttttacttc tctggttcag
    12601 gggaaaataa aagtttcatt ttcaactttt tcttgtcttg atgtaatata tatttctttt
    12661 gctcgtaggt gttcttcgta tgtttttgaa aaataatttt tcccatttcc cattgaaacg
    12721 aagaataaat agtcggtttt tttagggttt aatgctgcct gtaaagaaac tttaccagga
    12781 ttacatattg gagttggtgg aagtccttta tgaatataag tgttataagg cgattttact
    12841 tttaggtctt tcttatttaa tttttgttta tgttgcccaa ttgcatattc aacagtagca
    12901 caactttgaa ttggaagtcc tttttttaac cttttgtaaa ttattcctgc tacaattctc
    12961 ttgccttctg gatatgttgc ttctttctca actattgatg caatagttac aatttttttt
    13021 acctcattaa aattttcatc atttattctt tcacccttca agtcatacca gacatcatta
    13081 aatcttttcc acatagtata agtaattcct tcaatagata tttttaaagg gaaaaaatat
    13141 gtatcaggaa acaaaaaacc ctctaatttc ttttcttttg catgttttat gaagtcgtct
    13201 ttatttttta tcagtccttt tttttcaaga atatcagcga tttctactat gtttttccct
    13261 tccgggattg ttacttttac aagttttact tctcctttaa ctaacttttc aactactttt
    13321 tttaaaggag ttcttccaga aaattcatat attcctgatt ttaatttatt ttgaactttt
    13381 aatctgtttg tcaagaataa aaataatgat ggatttttta taactttttt ttgatataaa
    13441 atttttgcaa tctgggaagc attgagattt tcagggattt caacaagttt tgggggttta
    13501 acaagaggtg ggagtgtcat tatctttata atcccgagta aaataataag aaatgcaatt
    13561 ataataaaaa ttattttctt tttcataatt tcataatttc ttttcttaaa atattaagcc
    13621 ctgttcgtac tgctctttct ctaattgttg ctctatttcc attaaatata aatttttgtg
    13681 caaaattttt gttttttgga agtccaaccc ctatccatac aagtccaact ggcttttctt
    13741 tacttcctcc tgttggtcct gcaattcctg taaaagacaa tgaaatatcg cttttaccat
    13801 atttttttgc cccttttgcc atacaaattg ctacttcttc actcactgcc ccatattttt
    13861 taagagcaca ttttgggacc ctcaaaattc tttttttcaa ggtattgcta tatgttacaa
    13921 atccacctgt aaagtattca gaacttcctg gaatatcagt tataagttta caggcaagtc
    13981 ctcctgaaca tgactcggca attgcaagtt taagtttttt ttcctttaaa attttcccaa
    14041 taatttccgg aagggcaggg gggttaatac ctaaatagtt ttctccaaat ctttctttaa
    14101 aaatcttatc aatttcctct gttttttcct gataaattga taaatcaaat ataaacaaaa
    14161 gttcaattaa atctggatga gcaagaatta tgtgttcgct tttttctctt tcaattatgt
    14221 ccctcatttt ttcttctaca actgattcag gaacaccaca aacagaaaac tttgcaattt
    14281 taacattttc aattgaagag aatttgtttt ttatcttttc aatggtattt tctaacatag
    14341 gacctaattc atttggtggt cccggaagaa ctattataat tttgttattt tcttctataa
    14401 acatccctgg tgcagtccca acttcatttt ctattatttc acatccatca ataatataag
    14461 attgtttttt atttatttca ggaaggtccc gcccaatttt ttggaacctt tcttttattt
    14521 tttcccatat atagttagaa aaaataagtt ttctgttcaa aacatcactt attgcctctc
    14581 ttgtaaggtc atcaaaagtc ggtccaagtc cacctgttat tattaaaatt tcactttcta
    14641 ccaaagaatt ttctattgct tttttaattt cgtctttttt atccccaaca attatacatt
    14701 ttgatactct caatccactt ttaagaagaa tatcacttat caataaaata tctgtattta
    14761 atttatgaag cagaagttca cttcctacat ttatgatttc tattttgggg tatttttttt
    14821 ccattgattt aaaatttttc ttctttaagg attgtttttt tgattatatc aaaaatactt
    14881 acttttctat tctgaaaatc aattagccca caagatggct cattttttgg taaagaagga
    14941 cttcctggat ttaatattac tatattttct ttcttttcaa ttttccatag atgagtatgt
    15001 ccacttataa aaaaatttat attccatttt tttacagttt caaaaaaatt atcattaaaa
    15061 agatgcccat gtgtagcaat tattctataa ttttcaataa tccccaaaaa atatggagaa
    15121 acaaagggga ctagaagaaa tgtctggtca acttctgaat cgcaatttcc tttcactaca
    15181 aaaatcggtt tttggagatt attgagtttt tcaagtaact tttttggaca atatccttct
    15241 ggaattggat ttccaggacc atgattaaat atgtccccac aatgaattat aaaatcagta
    15301 tcttttaaaa tttcccatgc catttcccat ctttctaaac taccatgtgt atcacttatt
    15361 attcctattt ttctcatact tttattaaat ccgaattttt aaaaattgtc aactattacg
    15421 aggaaaatac ctgttttaag atttttttaa taactgaaaa tcatgcaaat tttaataact
    15481 atgcagttaa taccaatttg acaacggaca tagagtaaat attttc
//
LOCUS       DAOOVW010000038        15238 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_2609_length_15238_cov_8.297438, whole genome shotgun sequence.
ACCESSION   DAOOVW010000038 DAOOVW010000000
VERSION     DAOOVW010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15238
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2609_length_15238_cov_8.297438"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(436..1758)
                     /gene="melA"
                     /locus_tag="PLW95_06015"
     CDS             complement(436..1758)
                     /gene="melA"
                     /locus_tag="PLW95_06015"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOV22218.1"
                     /translation="MAKISFIGAGSFGFTRALVKDILTYPLLRDATISLMDIDKERLE
                     FIKRAVEKIIEVGKYPAKLEVTTDRKTSLKDADAVICTILAGGVHVWRYDIEIPKKYG
                     VDFNVGDTRGAAGIFRALRTIPVMLDICKDIEKQCPDAIFLNYTNPMAMLCRAMQRKT
                     SIKVTGLCHSVQGTAEMLAKWIGATMDQITYLCAGINHQAWFVEFKKDGKDAYPLIRK
                     VVKNNKEIYQEEIVRNEMFLSLGYYVTESSGHNSEYNWWFRKRQDLIEKYCTYGTGWN
                     PGKHAYILNEYLTREETWKNDIKKWLEEPISLERGHEYAASIINAWMGGEPFLFNGNL
                     PNTGIIPNLPYGACVEIPVLADKRGFNSIYVGSLPPQCAALNNVNISSEEMAVEGALT
                     GDAEMVYHAICYDPLSASVLSLAEIKKMVKEMFEKNKEYLPQFKHFRF"
     gene            complement(1770..4442)
                     /gene="ppdK"
                     /locus_tag="PLW95_06020"
     CDS             complement(1770..4442)
                     /gene="ppdK"
                     /locus_tag="PLW95_06020"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOV22219.1"
                     /translation="MEKKYIYFFGEGKAEGTGDMKDLLGGKGAGLAEMINAGIPVPPG
                     FTITTEMCTYFYQHNEKLPDGFDEELKKYLKRLEKATGKKFGSPENPLLVSVRSGAKF
                     SMPGMMDTILNLGLNDEVVKGLAKISGDERFAYDAYRRFIQMFGNVVMGIDKNKFEKY
                     LEEKKEEKGVKYDSELTGDDLKDIVKKYKQVYKEETGEEFPQDPMVQLKRAIEAVFKS
                     WNNPRAITYRKLNKIPDDLGTAVNIVTMVFGNMGYDSGTGVGFTRNPATGEKEFYGEY
                     LTNAQGEDVVAGIRTPKPISELKKEMPEVYNQLVEITQRLEKHYRDVQDFEFTIEKGT
                     LYMLQTRTGKRTPLAAVKIAVDMVEEGLITKEEGIMRVEPSQIDQLLHPILDPKVKVE
                     PIAKGLPASPGAASGKVVFTADEAEEKAKKEPVILVRVETSPDDIHGMNAAKGILTSR
                     GGMTSHAAVVARGMGKPCIVGCGALQVDEIRKIIKVGDIEIKEGEWITIDGGTGNVMF
                     GQVPTIEGGITGEFKKLMDWADEIRKIGVRTNADTPQDAKKAREFGAEGIGLCRTEHM
                     FFAPERLPIVQEMIMAETEEARRKALDKLLPFQKNDFKGIFKEMAGFPVIIRTLDPPL
                     HEFLPKREELMVEIALLKERKEDRKKVEEKERILKRVEQLHEFNPMLGHRGCRLGITY
                     PEITEMQARAIMEGACELAKEGIEVIPEIMIPLVGNVNELANQKEVVVRVAEEVMKKY
                     GVKIKYMVGTMIEVPRAALTADQIAKEAEFFSFGTNDLTQMTLGFSRDDAAKFLFYYL
                     DKKILNDDPFMTLDQEGVGQLVEMGIKKGRSTNPGLEVGICGEHGGDPESVKFCHRVG
                     MNYVSCSPYRVPVARISAAQAVIEEKLKDKWRDK"
     gene            complement(4510..5982)
                     /locus_tag="PLW95_06025"
     CDS             complement(4510..5982)
                     /locus_tag="PLW95_06025"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOV22220.1"
                     /translation="MRILAIGDIHYVSDSSYIGLPERKYEIGPEFVERILRREKENFD
                     VIVVCGDFVDNRTLSCTENDFLEIKKIIEKEKKPFILVRGNHDIEFSKFCKLSGNSTL
                     PLIYGEYIFYPFCDNYLKDEKCYRKKQEIEKFKKFSQQFRDKKIITIQHNIIYPKIEG
                     GYPYNFENDEEILNLYKENNVLVSISGHYHKGIKEKKHKGIYFLTLPAICESPFSYYI
                     IEINKEKIKFQKKSLCMEAPFPLIDFHCHTEFAYCRENVESKKNIERAKIFGLDGIVL
                     TEHSGQLYLSKDDYWGYKFLDGVDIIYQSKKEGKSRIEKFKEKIFPIKNSYVKVGMEV
                     ECDKNGKITLLPEDRNGIDYLIGAVHYLPEEYKVSYKKIKSGFLKFTEFVCRNNIDIL
                     AHPLRYFVRNKILPPEDIYSDVVKILKKYNVCAELNFHTNNPDPVFFERCIENGIKIS
                     LASDAHNLLEVGDFSKHIEFLKKIGVRSGENLLTKWVKFS"
     gene            complement(5979..6860)
                     /locus_tag="PLW95_06030"
     CDS             complement(5979..6860)
                     /locus_tag="PLW95_06030"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOV22221.1"
                     /translation="MKIYNIHQKIKIEEGLVIGIGKFDGVHLGHQKLIKKIISISKGK
                     NKIPAVFTFKNFPLESYITPFEEKISLLKKYGIEICIWSDFEEIKNWDGEKFIGFLIK
                     IGVKEIVVGFNFKFGKDRKWDVDLLKDISKKYGFSVNIVNAVEIDGEVVNTSKIKKFL
                     REGNIERANKFLGRHFSIKGKVVAGSGRGKKIGFPTANVLLDEKIEIGQGVYAGYVFY
                     KGEKYKGGLNVGNCPTFGEKRKRVEVYIFDFEQEIYGETIEVFFLKKIRDEIKFSSVD
                     ELKKQIKKDINIIKEIK"
     gene            complement(6864..7616)
                     /locus_tag="PLW95_06035"
     CDS             complement(6864..7616)
                     /locus_tag="PLW95_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HOV22222.1"
                     /translation="MKYEIIGSNLQFVKIYLSVGEEIYAEGGKMMFKSQNMNMESKIV
                     GDTRNDKLKGVFTRIVTGESLFTTHFTPINGDGYVAFAGDFPGKIQAIELKDGESFIS
                     QRDSFVCATKDVGFSIALQKKVGVAFFGGEGFILEKFTGPGTVFIHAGGDFYTVDLAP
                     GEKIHVDTGCIVGFEEKVNYDIEFVKDIKTAIFGGEGIFLATLTGPGKIILQSITLSR
                     LRREIGGKVVRKGGEKFGLGGIVGDILGGSED"
     gene            complement(7630..8400)
                     /locus_tag="PLW95_06040"
     CDS             complement(7630..8400)
                     /locus_tag="PLW95_06040"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOV22223.1"
                     /translation="MGKVNLIEMTLKEVREKGKIEVAVLPWGSTEPHNLHLPYGTDTL
                     AASKISEISAKKAIEKGAKVIVLPTIPVGVNSNTFGFPLVLHFSPTTQLSILKDIIWS
                     LENHKIYKLLLLNAHGGNDFKPLIREIFGKTNVWIFLLDWWNVRNDIVENVCEDKDGE
                     HGHEAETSWLMYLYPDLVHLEWADDGRVNKPRLKSLEEEKIWLPRQWHLLTTNSGHGN
                     PKKANAEKGKIIVEGATDRISEIILELSKTKIDEKFPY"
     gene            complement(8403..9581)
                     /locus_tag="PLW95_06045"
     CDS             complement(8403..9581)
                     /locus_tag="PLW95_06045"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HOV22224.1"
                     /translation="MGIYKIEDFDIKGDGKTINTEAIQKAIDTCNENGGGKIIFSSGI
                     FKTGTIELKSNVQLHLERGCKIIGSENPDDYRELEANGFLMQRIEPKKEMTKNALIIA
                     SESENISITGYGEINGSGLSFYKNTPSDENGKYNKPNIPRPRIIMFYKCKNVFIEGVK
                     FVDSPCWTIWFMKCEDINIHNIRIFGNKRMRNIDGIDVDACKNVIISDCIMDTEDDCI
                     AIRSMQNLYDEPAICENITVSNCILRTSCQGIRIGCPGDGIIQNCVFTNLIIEGARGI
                     ISQHPKIYLPENITDGADIHDIMFSNITIKSNLSPIWLYIENGIKLTRLSDINFSNIK
                     IIKSGEALIIKGNEETKIYNITFNNLEMENSGENGIVCCNCENIRFDNFKLTTKQEKE
                     "
     gene            complement(9759..10865)
                     /locus_tag="PLW95_06050"
     CDS             complement(9759..10865)
                     /locus_tag="PLW95_06050"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOV22225.1"
                     /translation="MKSSYFFMPGKVYFENEGIEKYLCEIKNYGKKVLIVSGKNFIFK
                     SGLFNRIKNVLEKEKIKFSHFYNVEPEPDTENVEIAVDFCKKENCDCILAVGGGSAMD
                     VGKGVAVRSKNPGKIEDYFGEKEYGNEPLPVIAIPTTSGTGSEVTRYSVIVYKKGRTK
                     KTIRSEKIIPKMAILDSSVLATLSSKLVAATGMDAFSHSVESFLSIKSDEFSEIFSTE
                     SLKILWEFLPYAGKGDEKSKEKVFFSSLLAGLAINKTGTIIVHGMGYSLTIKYGIHHG
                     TANALFLPYILEYLYNNGYKKEIEKLNKIWGSIENLSAFVKNMGLPFNLRDVGVKSED
                     IDELLELSKIGCERAIEGMKIKEIDFRKILELAF"
     gene            complement(10867..11928)
                     /locus_tag="PLW95_06055"
     CDS             complement(10867..11928)
                     /locus_tag="PLW95_06055"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HOV22226.1"
                     /translation="MDKFKNYEEKLSPSYFHLNLMKKTEKQLSFSKKSNFESWRKKLR
                     VKFKQLIGPIYKDKGKVDFKIIKEENHSDYKLQKIIFPTNPYVINVAYLLIPNKKKEK
                     YPAFVCLQGHSSGAHISVGVPYSKEDKEEINGDRDFAIQAVKNGFVALAIEQRCFGER
                     EEKLLKQKMENRCWDATMHSLMLGHTLISERISDVIRGIDLLYQFPFVDKRIIGCMGN
                     SGGGTITYYASCIEDRIKISVPSCSFCTYEDSIMKIGHCMDNYIPSSFKYFGMEDLAG
                     LIAPKFLIIVAGKEDNIFPIAGVRKAFNKAKEIYEKAGHPERIKLVVGPSGHRFYASL
                     AWKEIMKVKEEIIRKQMGE"
     gene            complement(11931..12800)
                     /gene="prmC"
                     /locus_tag="PLW95_06060"
     CDS             complement(11931..12800)
                     /gene="prmC"
                     /locus_tag="PLW95_06060"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOV22227.1"
                     /translation="MSQKMLSTELIKSGSEILKNNGIKEAVKKAEIILSEITKKKIPD
                     LYLDEEEISSRTIKKYIEYLNKIIEGIPLQYIIKKSYFYKYQFYVEKGVFIPRPETEI
                     IIEKTIEIYKNFFSPSFVNILDIGTGSGNIAITLAKEIKNSKIIATDVSEKSIKIAKK
                     NADRYKVENKIKFIKTDLFPKKNIKFDIIVSNPPYIPEREIENLENDVKKEPYIALNG
                     GKSGMEFIERILNNAGKYLSNNGFLILEIGYNQSKFLKNKKFNDLKIIEFTNDLNGIE
                     RVVIFKKHNKMEE"
     gene            12954..13277
                     /locus_tag="PLW95_06065"
     CDS             12954..13277
                     /locus_tag="PLW95_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22228.1"
                     /translation="MALTITLGFLGILIVLLGIGTNWALNIRAERLIQAEDERVKNLI
                     KEMREGTENLMKDMRQSTEISINNARQSTADLIREIKETKLYIASLIKSDGEKTRELI
                     NKVIK"
     gene            complement(13330..15171)
                     /locus_tag="PLW95_06070"
     CDS             complement(13330..15171)
                     /locus_tag="PLW95_06070"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOV22229.1"
                     /translation="MSIRKKFLIIMFFSSFILFSSDEFLDKVNSGLNYLKIGNYEDAL
                     SYFKQAKNLSPDNAIVYYYLGETYFRKGDMEEAKKYYDQAIEREPQNPKFHYSLAHLY
                     LAQNDKAKALEEFNKVIEISPQSLIGKNAKLVKKQIEKGIKEKDISDKWVKLEEEERK
                     KIAEEKKKKEAGKEQQGQPGMPGQEGMPGFEGIPGEKEEKIPIDQLVKRIKYGTKTLR
                     ENESRKLPYYSSSEISGVYNEIIGIIKKEKDIEVEKNLISALGKVNQPDVGEFILSLI
                     QNKETPFEIKIVALESIGGIRTESVMITLRDTLNNMIKNREQEREEAKKNIQNINNEI
                     EKLEVERITLNSELNKLQQDRSDISNKLQMGGMEPGMMAPGGGMPPVQGGGEIKVLST
                     TEVKKLRDQLKNIDTQIGTKTERLNEIQTQLQQLQEKSNKYQMLLAKKGGEVKISTPS
                     YVPQFTPGMFPPGMGPGFMPEYTPQQQKTTEEENEIIFAMKLIIALGEMRDKQALPVI
                     KNAWKEFGAPNYEPYYSLALAQLGDYSKIDLLLERLQQDYPQQEQEKNDEIKLRAGII
                     KILPDYLKTNPNEEIIGLINYLSENAQYPEISKAASEVLISLQTKGK"
BASE COUNT         4632 a         2909 c         1653 g         6044 t
ORIGIN      
        1 atataactat aaggtatttt ttttatctaa tttcaaatca ttgcttatta taatatattt
       61 tcccatctgt tctattgctt tatgaagaca atcttttatc aagaacaaat aaactttctt
      121 ttattccaaa agaattcacc tgtattagaa tattctttaa tgtggttaca tcttttatac
      181 tgcctgggta aatagtgaaa aaaacggcaa taatgatgac tcttcaaaaa ctataccttg
      241 acttaaaact acctctcact tttattattt gccctgtatt tatatcttgc ttccctaaat
      301 aatttctcct ttgtctcgat tgtttcttcc ctttatccca ataactttct ccttcatata
      361 cataaacatt cttccctatt ttctgttcta tttatataac tatgttatta atatcgcagg
      421 attttaggat aaaaattaaa aacggaaatg tttgaattgg ggaagatatt ctttattttt
      481 ttcaaacatc tcttttacca ttttttttat ttctgcaagt gaaagaacac tggctgagag
      541 agggtcataa caaattgcat gatataccat ttcagcgtct ccagttaaag caccttccac
      601 tgccatttct tcagaagata tattaacatt atttagtgct gcacattgag gaggtagtga
      661 ccctacatat attgaattaa atcccctttt atcagccaga actggaattt ctacacaagc
      721 accataaggg agattaggaa taatcccagt atttggaaga ttaccattaa ataaaaatgg
      781 ttctcctccc atccatgcgt tgataatact tgctgcatat tcatgtcccc tctctaagga
      841 aattggttct tctaaccatt ttttaatatc atttttccat gtctcttctc ttgtaagata
      901 ttcattaaga atatatgcat gttttcctgg attccaacca gttccataag tacaatattt
      961 ttctattagg tcctgtctct ttctaaacca ccagttatat tcagaattat gtccacttga
     1021 ttcagtaaca taatagccaa gtgacaaaaa catttcattt cttactattt cttcctgata
     1081 tatttcttta ttatttttta caacttttct gattaatgga tatgcgtctt taccatcttt
     1141 tttaaactca acaaaccatg cctgatggtt aattcctgca cataagtaag taatctggtc
     1201 catagttgcc cctatccatt tagcaagcat ttctgctgta ccttgaacac tatggcataa
     1261 tccagtaact tttatagatg tttttctttg cattgctcta cataacatcg ccataggatt
     1321 agtataattt aaaaaaattg catcgggaca ttgcttttca atgtctttac atatatcaag
     1381 cataacaggt atggttctta acgctctaaa aataccagca gcacctcttg tatctcctac
     1441 attaaaatcc actccatact ttttaggaat ttctatatca tatctccaga catgaacacc
     1501 acctgcaaga attgtgcaaa taactgcatc tgcatctttt aacgatgttt ttctatcagt
     1561 tgtaacttca agtttagcag gatatttacc cacttcaata attttttcta ctgccctttt
     1621 tataaattca agtcgttcct tgtcaatatc cattaaactt atagttgcat ctcttagtaa
     1681 tggataagtt aaaatatctt ttaccaaggc acgtgtaaaa ccaaaacttc ctgctccaat
     1741 aaatgatatt tttgccattt attctccttt tacttatctc tccatttatc tttcaatttt
     1801 tcttctatta ctgcctgtgc agcagaaatt cgtgcaacag gaactctata tggagaacac
     1861 gaaacataat tcattccaac tctatgacaa aattttacac tctcagggtc cccaccatgt
     1921 tctccgcata ttcccacttc aaggcctgga tttgtacttc tacctttctt aattcccatt
     1981 tctacaagtt gtcctactcc ttcctggtca agagtcataa atgggtcatc gttcaaaatt
     2041 tttttatcta aataataaaa taaaaattta gctgcatcat ctctgctaaa acctaatgtc
     2101 atctgtgtca aatcatttgt cccaaaagag aaaaattctg cttcttttgc aatctggtcc
     2161 gctgttaatg ccgcccttgg aacttctatc attgtcccaa ccatatattt tattttaaca
     2221 ccatattttt tcattacttc ttccgcaact ctcacaacta cttctttctg atttgctaac
     2281 tcatttacat ttcctacaag tggtatcata atttcaggaa taacttctat accttctttt
     2341 gcaagttcac acgccccttc cataattgcc cttgcctgca tttctgtaat ttcagggtat
     2401 gttattccaa gtcgacatcc acgatgtcca agcataggat taaattcatg tagttgttca
     2461 actcttttaa gaattctttc tttctcttct acctttttcc tatcctcttt cctctccttc
     2521 agtaacgcaa tttcaaccat taattcctct ctttttggta aaaattcatg caaaggtggg
     2581 tcaagagtcc ttataataac aggaaaacca gccatttctt taaaaatccc tttgaaatca
     2641 tttttttgaa atggtaataa cttatcaagt gcttttctcc ttgcctcttc tgtttctgcc
     2701 attatcattt cctgaactat tggtaatctt tcaggagcaa aaaacatatg ttcagttctg
     2761 caaagtccta ttccttctgc cccaaattct cttgcttttt ttgcatcctg tggagtatct
     2821 gcatttgttc taaccccaat tttccttatt tcatcagccc aatccattaa ctttttaaat
     2881 tctcctgtta tcccgccttc aattgtagga acttgtccaa acataacatt tccagttcca
     2941 ccatctattg ttatccattc cccttctttt atttctatat ctccaacttt tataattttt
     3001 cttatttcat ccacctgtaa agcaccacaa ccaacaatac atggttttcc catacctctt
     3061 gcaacaacag cagcgtgact ggtcatcccc cctctactcg tcaaaattcc ttttgctgca
     3121 ttcataccat gaatatcatc tggcgatgtt tcaactctta caagaataac tggctctttc
     3181 tttgcttttt cttctgcttc atctgctgta aatacaactt tcccacttgc tgctcctggt
     3241 gaagcaggaa gtccttttgc aattggttca acttttactt ttgggtcaag aattggatga
     3301 agtaattggt caatctgaga tggttcaact ctcattatgc cctcttcttt tgtaattaat
     3361 ccttcttcaa ccatatccac tgctatttta accgctgcca gtggtgtcct cttaccagtt
     3421 cttgtttgaa gcatataaag agtacctttt tcaattgtaa attcaaagtc ctgaacatct
     3481 ctgtaatgtt tttcaagccg ttgtgtaatt tctactaatt gattataaac ttctggcatt
     3541 tcttttttaa gttcacttat tggttttgga gtccttattc ccgcaacaac atcttctccc
     3601 tgtgcatttg ttagatattc cccataaaat tctttttctc ctgttgccgg gtttcttgta
     3661 aatccaacac ctgttcctga atcatatccc atatttccaa aaaccattgt tacaatatta
     3721 actgctgttc ctaaatcatc aggtatctta tttaattttc tgtatgtgat tgctcgtggg
     3781 ttattccatg atttaaagac ggcctctatg gctcttttca attgaaccat tgggtcctgt
     3841 ggaaattcct cccccgtttc ttctttatat acttgtttat attttttaac tatatctttt
     3901 aggtcatcac ctgttaattc agaatcatac tttactcctt tttcttcttt cttttcttct
     3961 aaatattttt caaacttatt tttatctatt cccataacaa catttccaaa catctgaata
     4021 aatcttctat aagcatcata agcaaatctt tcatcacctg aaatttttgc cagccccttt
     4081 actacttcat cattaagccc taaatttaaa attgtatcca tcatacctgg catggaaaat
     4141 tttgctcctg accttactga aacaagaagt ggattttcag gagaaccaaa ttttttcccg
     4201 gtagcttttt caagtctttt tagatatttt ttaagttctt catcaaaacc atcaggaagt
     4261 ttctcattgt gttgataaaa gtaagtacac atttctgttg taattgtaaa accaggtgga
     4321 actggtattc ctgcatttat catttctgca agtcctgctc cttttccacc aagtaagtcc
     4381 ttcatatctc ctgttccttc tgcttttcct tcaccaaaaa aataaatata ttttttctcc
     4441 atctttttcc tccttgatat caatatttta ttttttaatt ctgtatgata actttttttg
     4501 atgtataagt caactaaatt taacccattt tgttagcaaa ttttcgccag aacgaacacc
     4561 aatttttttt agaaactcaa tatgttttga aaaatcccct acttcaagta agttatgagc
     4621 atctgaggca agagaaattt taataccatt ttctatacat ctttcaaaaa aaacagggtc
     4681 tgggttattg gtatgaaaat ttaattctgc acacacatta tatttcttca agatttttac
     4741 aacatctgaa taaatatcct ctggtgggag tattttattc cttacaaaat atcttaaagg
     4801 atgagcaaga atatctatat tatttctgca tacaaattcc gtaaatttta aaaatccact
     4861 ttttattttt ttataactta ctttatattc ttccggtaaa taatgaactg ctcctattaa
     4921 ataatcaata ccatttctat cttctggtaa aagtgttatt ttcccatttt tatcacattc
     4981 aacttccatt ccaactttta cataactatt tttaatagga aatattttct ctttaaattt
     5041 ttctattctg cttttccctt ctttttttga ctgataaatt atatccaccc catctaaaaa
     5101 tttataaccc cagtaatcat cttttgaaag ataaagttgc cctgaatgtt ctgtaagaac
     5161 aataccatca agtccaaaaa ttttcgccct ttctatgttt tttttactct ctacattctc
     5221 tctacaataa gcaaattcag tatgacaatg aaaatcaatt aaaggaaatg gtgcttccat
     5281 acataaggac tttttttgaa acttaatttt ttctttattt atttctatta tgtaatagga
     5341 aaatggactt tcacatatag caggtaaagt taaaaagtaa attcctttat gttttttttc
     5401 ttttattcct ttatgataat gtccacttat agacactaaa acattattct ctttatataa
     5461 atttaaaatt tcttcatcat tttcaaaatt ataaggataa ccaccttcta tttttggata
     5521 aataatatta tgctgaatag taataatttt tttatctcta aactgctgag agaatttttt
     5581 gaatttttca atttcctgtt tttttctata acatttttca tctttcaggt aattgtcaca
     5641 aaagggataa aaaatatatt ccccataaat taaaggtaat gttgaatttc cggataattt
     5701 acaaaattta ctgaattcaa tatcatggtt tcctctgacc aaaataaatg gttttttctc
     5761 tttttcaata atttttttaa tttcaagaaa atcattttct gtacatgaaa gagtcctatt
     5821 atcaacaaaa tcaccacata caacaataac atcaaagttt tctttttctc tccttaaaat
     5881 tctttcaaca aattcaggac caatctcata ttttctttca ggaagtccaa tataactact
     5941 atcagaaaca taatgtatat cacctattgc taaaattctc attttatttc cttaattata
     6001 tttatgtctt tcttaatttg ttttttcaat tcatcaacag atgaaaattt tatctcatct
     6061 ctaatttttt tcaaaaaaaa cacctcaatt gtttctccat aaatctcttg ttcaaaatca
     6121 aaaatataaa cttcaactct ttttcttttt tctccaaaag tgggacaatt cccaacattc
     6181 aatccccctt tgtatttttc ccctttataa aaaacatatc cagcataaac accctgccct
     6241 atttcaattt tttcgtctaa cagaacattc gcagtaggaa aacctatttt tttacctctg
     6301 ccactgccag caactacttt tcctttaatg gaaaaatgcc ttcctaaaaa cttatttgct
     6361 ctttcaatat tgccttctct caaaaatttt tttattttac tcgtatttac aacttctcca
     6421 tctatctcaa ctgcattaac aatattaact gaaaatccat atttttttga aatatctttt
     6481 aataaatcaa catcccattt tctatccttc ccaaatttaa aattaaaacc aacaacaatt
     6541 tcttttactc ctattttaat tagaaatcca ataaattttt ctccatccca attttttatc
     6601 tcttcaaaat cactccatat gcatatttca attccatatt ttttcaatag agaaattttc
     6661 tcttcaaatg gagtaatata agattctaac gggaaatttt taaaggtaaa aacagcagga
     6721 attttatttt ttccttttga aatagaaata atttttttta ttaacttttg atgtcctaaa
     6781 tgaaccccat caaattttcc aatgccaata actaaaccct cttcaatttt tattttttga
     6841 tgaatattgt agattttcac aaattaatcc tcacttccac ccaaaatatc tcctactatt
     6901 ccaccaagtc caaatttttc tccgcctttt cttacaactt ttcccccaat ttctcttcta
     6961 agacgcgata atgtaatact ctgtaaaatt atcttgccgg gacctgttaa tgttgcaaga
     7021 aatatgcctt ctcctccaaa aattgctgtt tttatatctt ttacaaattc aatatcataa
     7081 ttaacctttt cttcaaaccc aacaatacaa ccagtatcaa catgtatttt ttctccaggt
     7141 gctaaatcaa ctgtataaaa atcacctcca gcatgaataa aaactgtgcc aggacctgta
     7201 aatttttcaa gaataaatcc ttccccacca aaaaatgcaa caccaacttt tttttgaaga
     7261 gcaattgaaa aaccaacatc cttagttgca caaacaaatg aatctctttg agaaataaaa
     7321 ctttcaccat ctttaagttc tattgcttga attttaccgg gaaaatcccc agcaaaagca
     7381 acatatccat ctccatttat aggagtaaag tgagttgtga acaaactttc tcctgttact
     7441 atcctcgtaa aaactccttt taatttatca tttcttgtat ctccaacaat tttgctttcc
     7501 atattcatat tttgactttt aaacatcatt ttcccacctt ctgcataaat ttcctctcct
     7561 actgaaagat atatctttac aaattgcaaa ttactaccaa ttatttcgta tttcattttt
     7621 ttccctccat tagtaaggaa atttttcatc aatttttgtt tttgaaagtt ctaaaattat
     7681 ttctgaaatt ctatctgttg ctccttcaac aattattttt cctttttctg catttgcctt
     7741 tttaggattt ccatgaccag aatttgtcgt taaaagatgc cattgtcttg gaagccatat
     7801 tttctcttct tctaatgatt taagtcgtgg tttatttact cttccatcat cagcccattc
     7861 aaggtgaaca aggtctggat aaagatacat caaccatgat gtttctgcct catgtccatg
     7921 ttctccatct ttatcttcac agacattttc aacaatatca tttctaacat tccaccagtc
     7981 aagtaaaaaa atccagacat ttgttttgcc aaaaatctct cttattaatg gcttaaaatc
     8041 atttccacca tgagcattaa gaagtaaaag tttataaatt ttatgatttt caagtgacca
     8101 gataatatct tttaaaattg aaagttgagt tgttggagaa aaatgtaata ccaaaggaaa
     8161 accaaaagtg tttgaattaa caccaactgg aatagttggt aatacaataa cttttgcccc
     8221 tttttctatt gcctttttag cacttatttc actaatttta gaagcagcaa gtgtatctgt
     8281 tccataagga agatgtaaat tgtgtggttc tgttgaaccc catggcaaaa cagcaacttc
     8341 aattttccct ttttctctta cttcttttaa agtcatctct attaaattta cttttcccat
     8401 atttactcct tttcctgttt agttgttaat ttaaaattat caaatctaat gttctcgcaa
     8461 ttacaacata ctattccatt ttctcctgaa ttttccattt ctaaattatt aaaagtaata
     8521 ttataaattt tggtttcttc atttcctttt ataataagtg cctctccact tttaattatt
     8581 tttatattgg aaaaatttat atctgaaagt cgtgttaatt taataccatt ttctatatat
     8641 aaccatattg gtgataaatt acttttaatt gtaatgtttg aaaacataat atcgtgaata
     8701 tctgcaccat ctgtaatatt ttctggtaaa taaatttttg gatgttgaga aataatacct
     8761 cttgcacctt caataattaa atttgtaaat acacaattct gtattatccc atctcccgga
     8821 caccctattc ttattccttg acaagaagtt cttaaaatac aattgcttac agtaatattt
     8881 tcacatatgg caggttcatc atataagttt tgcatagaac gaattgctat acaatcgtct
     8941 tcagtatcca taatacaatc actaattatt acatttttac atgcatctac atctattcca
     9001 tcaatatttc tcatcctttt atttccaaaa attcttatat tatgaatatt tatatcctca
     9061 cacttcataa accagattgt ccaacatggt gaatcaacaa attttactcc ttcaataaaa
     9121 acatttttac atttataaaa cattattatt cttggtcttg gaatatttgg tttgttatac
     9181 ttaccatttt catcggatgg ggtattttta taaaaagaaa gtcctgaacc attaatctct
     9241 ccatatcctg taattgaaat attttcactt tcactggcaa ttataagagc attttttgtc
     9301 atttcctttt ttggttctat tctctgcatt aaaaatccat ttgcctctaa ttctctatag
     9361 tcatcaggat tttcacttcc aataatttta cacccccgct ctaaatgaag ttgaacatta
     9421 cttttaagtt ctattgttcc tgttttaaaa attccactac taaatataat ttttcctcct
     9481 ccattttcat tacaggtatc tattgctttc tgaattgctt ctgtgttaat tgtttttcca
     9541 tctcctttta tatcaaagtc ctcaatttta tatatcccca tattttctta tccttttttt
     9601 aaaaaatatt atcatatttt gtagagaaat taaatcaatt tttaaatact caatcaatac
     9661 taaaatataa ttaggttgta gtaaataatt atatatattg ttgagtttta ataataaaat
     9721 aattcaaaac ttcagaagtt atggagttaa gaaaaaaatt aaaaagccaa ttccaatatt
     9781 tttctaaaat ctatttcttt tattttcatt ccttcaattg ctctttcaca accaattttt
     9841 gaaagttcta aaagttcatc tatatcttca ctttttaccc caacatctct taaattaaaa
     9901 ggcaatccca tatttttaac aaaggcactt aaattttcaa tacttcccca tattttattt
     9961 aatttttcaa tttctttttt ataaccatta ttataaaggt attctaaaat ataaggaaga
    10021 aatagagcat tagcagttcc atgatgaatt ccatatttta ttgtaagtga atatcccatc
    10081 ccatgaacaa ttattgttcc tgttttattt attgccagtc cagcgagaag agaagaaaaa
    10141 aatacttttt ctttactttt ttcatctccc ttacctgcat aaggcaagaa ctcccataaa
    10201 atttttagcg attcagttga aaaaatctct gaaaattcat cacttttaat agaaagaaaa
    10261 ctttctacac tatgtgaaaa agcgtccatc ccagttgcag caacaagttt agaagaaagt
    10321 gttgccagaa ctgatgaatc taaaattgcc attttgggta taattttttc acttcttatt
    10381 gtttttttag tccttccttt tttataaaca ataactgaat atcttgtaac ttcacttcct
    10441 gtcccagatg ttgttggaat tgcaattact ggtaatggtt catttccata ttccttttct
    10501 ccaaaataat cctcaatttt ccctggattt ttacttctta ctgctacacc tttaccaaca
    10561 tccattgcac tcccaccacc aactgcaagg atacaatcac aattttcttt tttacaaaaa
    10621 tcaactgcaa tttcaacatt ttctgtatct ggttctggtt caacattata aaaatgagaa
    10681 aattttattt tttccttttc aagtacattt ttaattctat taaaaagtcc tgatttgaaa
    10741 ataaaatttt ttccacttac aattagaact ttttttccat aattttttat ttcgcaaaga
    10801 tacttttcta taccttcatt ttcaaaataa acttttcctg gcataaaaaa atatgatgat
    10861 ttcatcttat tcccccattt gttttcttat aatttcctct ttaactttca ttatctcttt
    10921 ccatgctaat gatgcataaa atctatgtcc agaaggtcca actacaagtt taattctttc
    10981 aggatggcct gctttttcat atatttcctt tgctttgtta aatgcctttc taacacctgc
    11041 aatcgggaaa atattgtcct cttttcctgc aacaataatt aaaaatttag gtgcaattaa
    11101 accagcaagg tcttccatac caaaatattt aaaagatgat ggaatataat tatccataca
    11161 atgtcctatt ttcataattg agtcctcata tgtacaaaaa gaacaggaag gaacagaaat
    11221 ttttattcta tcctcaatac aggaagcata ataagttatt gttccaccac ctgaattgcc
    11281 catacaacca attattcttt tatcaacaaa tggaaactgg tataacaaat caattcccct
    11341 tataacatcg gatatccttt cacttattaa agtatgccct aacattaaag aatgcatagt
    11401 agcatcccag cacctatttt ccattttctg ttttaataat ttttcttctc tttctccaaa
    11461 acatctctgt tcaatagcaa gtgcaacgaa tccattcttt actgcctgta ttgcaaaatc
    11521 tctatctcca tttatttcct ctttatcttc ttttgaataa ggaacaccaa ctgaaatatg
    11581 agcaccagaa gaatgccctt gaagacaaac aaaagcagga tatttttctt ttttcttatt
    11641 tggaattaaa agatatgcaa catttattac ataaggattt gttggaaaaa ttattttctg
    11701 taatttataa tcagaatgat tttcctcttt tataatttta aaatcaactt tccctttatc
    11761 tttataaatt ggtcctataa gttgtttgaa ttttactctt aatttttttc tccaactttc
    11821 aaaattactc ttttttgaaa aagaaagttg cttctccgtc tttttcatca aatttaagtg
    11881 aaaatacgat ggagaaagtt tttcttcata atttttaaat ttatccattt ttattcctcc
    11941 attttattgt gtttcttaaa aattactact ctctcaatgc cattcaggtc atttgtaaat
    12001 tctataattt ttaaatcatt aaatttttta tttttcaaaa atttactttg attataccct
    12061 atttctaaaa ttaaaaagcc attattagat aaatattttc ctgcattgtt aagaattctt
    12121 tctataaatt ccattcctga tttcccacca tttaaagcaa tatatggttc tttttttaca
    12181 tcattttcta aattttctat ttctctttca ggtatataag gaggattact aacaattata
    12241 tcaaatttaa tattcttttt tggaaatagg tctgttttta taaacttaat tttattttca
    12301 actttatatc tatcagcatt tttttttgca attttaatac tcttttctga aacatctgtt
    12361 gcaataattt tagaattttt tatttctttt gcaagggtaa ttgcaatatt tccagaacca
    12421 gttcctatat caagaatatt aacaaacgaa ggggaaaaaa aatttttata aatctcaatc
    12481 gttttttcta tgattatttc tgtttcaggt cttggtataa aaactccctt ttcaacataa
    12541 aattgatatt tataaaaata gctcttcttt attatgtatt ggagaggaat cccttcaatt
    12601 attttgttaa ggtattcaat atattttttt attgttcttg aagaaatttc ttcttcatct
    12661 aagtaaaggt ctggaatttt cttttttgta atctcactta aaataatttc tgccttttta
    12721 actgcttctt taatcccatt attttttaaa atttcacttc ctgattttat tagttctgtg
    12781 gataacattt tttgagacat tatttagaaa tttgaaagta tctattttaa ataatttacg
    12841 ccttttccct ttcttcgtca ataaaatttt ttctggaacc tggttccaga attgtatttg
    12901 ccaggttgtt tttatatcag gtataataac tcaaaggaga aggaaaaata aaaatggctc
    12961 ttacaataac tcttgggttt ttaggtattt taattgtttt gcttggaata gggacaaatt
    13021 gggctttaaa tataagggca gaaagattaa tacaggcaga agatgaaaga gtaaaaaatt
    13081 taataaaaga gatgagggaa ggtacagaaa atctaatgaa agatatgaga caaagtacag
    13141 aaatttcaat aaataatgca agacaaagca cagcagattt aataagagag ataaaagaga
    13201 caaaacttta tattgcctct cttataaaaa gtgacggtga aaaaacaaga gaattgataa
    13261 acaaagtaat aaaataaaaa aatatttgtt gatcttatcc aaccccgtag ttgaatctac
    13321 caaaaaaaat tatttacctt tggtctgtaa agaaataaga acttctgagg cagctttact
    13381 gatttccgga tattgggcat tttcacttaa atagtttatt aaaccaatta tttcctcatt
    13441 tggattggtt ttcaaataat ctggtagaat ttttataata cctgccctca attttatctc
    13501 atcatttttt tcttgttcct gttgtgggta gtcctgctgt aatctttcaa gaagtaggtc
    13561 aattttgcta taatcaccta attgggcaag tgccaatgaa taatatggtt cataatttgg
    13621 ggcaccaaat tctttccatg catttttaat tacaggaagt gcctgtttat ctctcatttc
    13681 tccaagtgca ataattaatt tcatagcaaa aataatttca ttttcttctt cggttgtttt
    13741 ttgttgttgc ggagtatatt caggcataaa tcctggtccc ataccaggag gaaacatacc
    13801 aggagtaaat tgagggacat aagaaggtgt tgaaatttta acttcccctc ctttttttgc
    13861 taataacatt tgatatttat tactcttttc ctgtaattgt tgtaattgag tttgtatctc
    13921 atttaatctt tctgtctttg ttccaatctg tgtatcaata tttttcaatt ggtctcttaa
    13981 tttcttaact tctgttgttg agagaacttt tatttctccc ccaccttgaa caggaggcat
    14041 accaccacct ggtgccatca ttcctggctc cattcctccc atctgtaatt tgttacttat
    14101 atcactccta tcctgttgaa gtttattcag ttcactgttt aatgtaattc tttcaacttc
    14161 taatttttct atttcattat ttatattttg aatattcttt tttgcctctt ctctttcctg
    14221 ttctctattt tttatcatat tattaagtgt atccctgaga gttatcataa cagattcagt
    14281 tcttattcct cctatacttt caagagcaac aattttaatt tcaaatggtg tctctttatt
    14341 ctgaattaaa gaaagaataa attcgccaac atctggttga ttaactttac ctaatgcact
    14401 tattaagttc ttttccactt caatatcttt ttctttttta attattccaa ttatttcatt
    14461 atagacacct gaaatctctg atgaagaata ataaggtaat tttcttgatt cgttttctct
    14521 taatgttttc gttccatatt ttattctttt tactaattgg tcaattggaa ttttttcttc
    14581 tttttccccg ggaatacctt caaatccagg cataccttct tgtcctggca ttcctggttg
    14641 tccttgctgt tctttccccg cttctttttt cttcttttcc tctgctattt ttttcctttc
    14701 ttcttcttct aattttaccc atttgtcaga tatatctttt tctttaatcc ccttttctat
    14761 ttgtttttta actaatttag catttttgcc aattagtgac tgaggagaaa tctctataac
    14821 tttattaaat tcttcaagtg cttttgcctt atcgttttga gcaagataaa ggtgagcaag
    14881 agaataatga aatttaggat tttgtggctc tctttcaatt gcctggtcat aatatttctt
    14941 tgcttcctcc atatctcctt tcctaaaata tgtttctcct aaataataat aaacaattgc
    15001 attatcagga cttagatttt ttgcctgttt aaaatagctg agtgcatctt cataattacc
    15061 tattttaaga taattcaaac cagaatttac tttatcaaga aattcatcag aagaaaaaag
    15121 aataaatgat gaaaaaaaca taatgattaa aaattttttt ctaatagaca tttttacctc
    15181 ctctattata ctattactat ttagtaagta ttcaatttta tatctctctt aaaatccc
//
LOCUS       DAOOVW010000039        14649 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_2738_length_14649_cov_8.214198, whole genome shotgun sequence.
ACCESSION   DAOOVW010000039 DAOOVW010000000
VERSION     DAOOVW010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14649
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2738_length_14649_cov_8.214198"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..273
                     /locus_tag="PLW95_06075"
     CDS             85..273
                     /locus_tag="PLW95_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22230.1"
                     /translation="MERFKTLISKNPHLIKITLSNIFTMRLIRNKIHGDLEEIALAEF
                     INQYMYDYKSEHIGKQKF"
     gene            456..620
                     /locus_tag="PLW95_06080"
     CDS             456..620
                     /locus_tag="PLW95_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22231.1"
                     /translation="MQILESEEFSGFNDLNILPLIYDEKNKKCNIMVFDLDKAKKERE
                     YKNKFWEIFG"
     gene            635..1531
                     /locus_tag="PLW95_06085"
     CDS             635..1531
                     /locus_tag="PLW95_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22232.1"
                     /translation="MRKVICLVGFVLLFFGGLHREYILWGSEENIKEILTMEEWNRYQ
                     ELTGIQSKKKEVSGKYWQAVGPSFDVANPKDKEPAQLYKEELEKYDKILKEKSREFFE
                     ILIPQKEKIFLLAVEIDIKDSESAGWILAKGFSDIESNPETFLKAYVCLNKYEDRMQR
                     KEMRDSMRNGIFYLVDKSTFEQEENRIRFANNFLLSEGSIEDKKITAKFLWEVWSGFF
                     PRIERMENKSEAYEKMNQQSLSIIETLNSIKTDIKLTEEVNKVLQNFHAHYSRKDLPV
                     EFLQSSSISNVKEKIAQSMKKD"
     gene            complement(1721..1906)
                     /locus_tag="PLW95_06090"
     CDS             complement(1721..1906)
                     /locus_tag="PLW95_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22233.1"
                     /translation="MKETCQQLQEDYYGTHGLRWNYAQEQHQENMENGMTYEQSLNET
                     SEKMGHSRADITEHYLK"
     gene            complement(2332..3495)
                     /locus_tag="PLW95_06095"
     CDS             complement(2332..3495)
                     /locus_tag="PLW95_06095"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HOV22234.1"
                     /translation="MENYSYEISKIPVIINFSHFCGKSGTDEYHLVFIPKEYGNFENQ
                     IKWVYTAYKETLKYIGLDEETSVFKRFYCSDLFNQAIYLKNKNLFNFSKCGLSYICQP
                     PGPCGKVALWSYHIKDKLNKTKKKLKNNNFSFKRGNIIHNLTTGITYPEGKTVYEQTT
                     GVLKKYNNFLLKNKMTLLNNVIRTWFFIQNIDLNYKEFVDARREFYAQNGLTKDTHFI
                     ASTGVEGKYFDSDVKVVMDAYSILGISKEQVEYLSAPEYLGPTYLYGVTFERGVAISY
                     KDRKHIIISGTASINNKGEILYYGNITKQLERCLENVHALLKKANAKLSDISLLVVYI
                     RDPSDYTIVKKKIREKFLNTPLIICYASVCRPGWLIEIECFATIPLSTSVFYC"
     gene            complement(3505..5640)
                     /locus_tag="PLW95_06100"
     CDS             complement(3505..5640)
                     /locus_tag="PLW95_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HOV22235.1"
                     /translation="MKGIKTIFIFIFIFGFTLIAQSVSQPSIGYIYPSGGKKGSSVQI
                     LVGGQNLRGVKDICCSSDGIMAETIIYIPVLNNMQRNYLLKKISELKRKNKGIYQEKK
                     LSQDEIVELPDNPLLKNLENLSPEELDKIFEIFLMPIQRVQIKRSIQEKVLIYLKVDS
                     DVEPKKYEIRLLTQRGLTNPLFFEVNTFNEINEKEPNGPIEQQKNILDLPIIVNGRIM
                     PGDVDRFWFNAKKGQFLFIELKARDIIPYMADAVPGWFQGILTLYKSKGKEIFYADNY
                     YFNPDPIIFYEVEEDGEYIIEVRDALFRGREDFVYRLIIGEKSAFKNNFQEIKNVINS
                     DILNKLPEICEKEPNDKLNKANYIILPQIIKGIIEKPGDIDTYKFKGEIGEEIIFDVY
                     SRRLGYPLDSFIRILDNSGKTLKFNDDYEDKSFGLITHNADSYTSFVVPKDGYYYLQI
                     LDIQNHGGDNYLYYIRIENPEPDFKLFVIPSSINILAGRNNILNIYAIRKDGFDGEIK
                     ISLKEPNPNFILNGGRIPKGKDNVRITISSLKAPYENPFDIQIEGSTIIEGKEVKRIA
                     IPAEEMMQAFAYFHLVPFEKNFCYVINRGKISQFDFKLIDEDILKIPIDGVVNVKVKT
                     LQKLKSDEISLELKEPPKEISIEDVKIENGILSFNLRTNGKEIKSGFADNLIIEVFRE
                     NKTSTPEKPVQKVEKVSIGFLPAISFEII"
     gene            complement(5627..6916)
                     /locus_tag="PLW95_06105"
     CDS             complement(5627..6916)
                     /locus_tag="PLW95_06105"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HOV22236.1"
                     /translation="MKNKIFISFLVFNVLFTLMGFGEEEKSFDKKAKAVIQIWLWGGP
                     SHLDTFDPKPNAGYDYTGPFQAIPTNVPGIEINELLPNLAKIADKYSIIRSITHGITA
                     HETASYIMQTGHSSDERVLYPSIGSAVTFFKGKEYKGIIPPYIVLTTSQGRFSEEGFL
                     GQKYKPFVTGGDPNRTPFAVEGIVVEGISEERNLRRTKLLNSLDTLGSIASLEEFQSF
                     DSCKEEAYKIVNECKKIFDISKEPEDLRNMYGRNKFGQSCLMARKLIENGVIYVTINY
                     PGWDTHSNHFQQMRQKLPELDQGLSALIKDLSERGLLENTIIWVSGEFGRTPKISWEP
                     PWNGGRGHYGNCFSVLVAGGGFKGGIVLGKSDEKGEYVIERPVYPKDLLGTIYYLLGI
                     DPEGIFPDIGKGEIRVLPPIEKGTGKEILKEIINEGN"
     gene            complement(6925..8514)
                     /locus_tag="PLW95_06110"
     CDS             complement(6925..8514)
                     /locus_tag="PLW95_06110"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HOV22237.1"
                     /translation="MKVKIICFLLICFIVNGCFAQKENNLFYSEKIISTYEKNSWDIP
                     ETEIDKYVFSNLKKQGIEPALECSDEVFIRRVYLDLIGKLPENQEVISFLNDKNPDKR
                     SLLIDALLEKDDFADYWSMKWCDILRVKSEFPINLWPNAVQSYHKWILNSLKENKHYD
                     KFVRELLTSSGSNFYTPPVNFYRAIQGRDPYSITSAVALTFMGVRFDKLPQKTKDEMS
                     KFFSRVSYKNTLEWKEEIVYFNPEPSETIQCTFPDGEKVIIPSGIDPRIVFADCLINP
                     KNQWFCKNIVNRIWAYLLGTGIINEVDDIRPDNPPSNPELIIYLEKQLVNSNYDLKHI
                     YRLILNSRTYQQSFIPKTNNPEAKKYFAYYQIKRIDAEVLLDILCNIGGDGVEYISQI
                     PEPYTFIPKYQKNVLLADGSITSPFLEMFGRPSRNTGLFSERENKITEVQIRYLLNSS
                     EIQKKIKSSPYLKRIIRETKGNRKEIIRNIYLFLLSRYPEQSEIDTLEKYFQDGKVNL
                     NEGVEDIVWAIINSKEFLYNH"
     gene            8661..9797
                     /locus_tag="PLW95_06115"
     CDS             8661..9797
                     /locus_tag="PLW95_06115"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HOV22238.1"
                     /translation="MKKKRLFTPGPTSVPEEILLEMAKPIMHHRTDEFRAIVKDVSNG
                     LKYIFQTENSVFIIGSSGTGAMETAITNVFSKGEKIAVVCCGKFGERFAEIGNVYGLK
                     ILPVEIEWGKTLKAEELEKLLRENPDIRGICTPLCETSTGTAFDIENYGKVISKFPDV
                     ILIVDGVSSVGAIPCYMDKWGIDVLITGSQKALMLPPGLSFIALSKKAWGKTENSDIP
                     KYYFDLKKYKKAIEKDDFPFTIPVSLVIGLKRSVELFREYGLENLWSLHSKRANATRK
                     GIEKMRLELLSENPSDAVTAIKLPEEIDGEKLVKNLRTKYGISVAGGQDKLKGKIIRI
                     SHLGYMDEFDVLTAIIAVGICLEEMGYKCNLEDGIREAKQVLFS"
     gene            9818..10681
                     /gene="nadC"
                     /locus_tag="PLW95_06120"
     CDS             9818..10681
                     /gene="nadC"
                     /locus_tag="PLW95_06120"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013886155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOV22239.1"
                     /translation="MEKEVEKIIKKAIKEDLGKGDVTTSAIFKEKFPVKAILISKGDG
                     ILCGVDIFKNVFNILSSSFSFNFSFKDGDKIKKKDIVGEIYGPIKELLIGERTALNFL
                     QHLSGIATETRKLVERAKNKVKIYDTRKTIPNLRLLEKYAVKIGGGENHRFGLFDMVL
                     IKDNHIKGVMEKEKVDKISAISICVKRAKNYWKNKIKIEVEVENFKQAISAYQAGADI
                     IMFDNADKNQLLEFNKFLKGKKKVEIEWSGNVSLKKIDKIKKLPVDRVSAGYITHSAK
                     VLDFSLKISSI"
     gene            10763..11542
                     /locus_tag="PLW95_06125"
     CDS             10763..11542
                     /locus_tag="PLW95_06125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOV22240.1"
                     /translation="MKYFDTHCHLDFSDYDSDRDKVIEKSKNEGIEYIINVGVNLESS
                     KKSIKIASRYKFIFVGVGIHPSDVYQCNKKDIFEIEKLSKEKKVVAIGEIGLDFYYNK
                     DNREKQTEFFITQLKIASENSLPVIIHQRESKEEIIEILEKMNKNLPEKIVFHCFGED
                     NVFAKYCKEKNFYISFTGIITFKNANGIREIAKDFPLEKIMSETDSPYLSPTPFRGKR
                     NEPSNVRYIVEEIANQKKQSIEEVSNVIFKNSVTFFSIPSY"
     gene            11647..12813
                     /locus_tag="PLW95_06130"
     CDS             11647..12813
                     /locus_tag="PLW95_06130"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HOV22241.1"
                     /translation="MEFITFGCKVNQYETQLLKENFPYIDIMPDNVCVINSCCVTNKV
                     EKEVKTVIRKKLKKGKNVWLTGCFVNEEKIKLLFPSIKIFNKFEFCKKVRIIKSFNNH
                     TRGFVKIEDGCENNCSYCIIPLVRGKVKSRDEEEIIDEIEKLTENGYKEIVLTGIDLG
                     AYGKDNGKKITTLIEKISQVKDLKRIRLSSIEAVYINETLIDFLSSLDIFCPHFHIPL
                     QSGSDKILRLMKRNYNLSQYLKKIEVIKEKIRNVTFTTDIIVGFPGEEEDDFKQTCKA
                     IGEINFLKVHIFPFSPREGTKAYFMGNKVDYKVKKEREKVLSYSVKKEREKVMKKFLF
                     SSLSVLFEKKEENLWTGYSENYIPFSVISDEDIKNEIVKVIGKEIRNDKIYGII"
     gene            12827..13012
                     /locus_tag="PLW95_06135"
     CDS             12827..13012
                     /locus_tag="PLW95_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22242.1"
                     /translation="MEKFEIVNLVSGVLLIVLGCFFTFKPKLTYQKEGISDIFAVFGI
                     ILMVIGVVLLFSPLLNR"
     gene            complement(13001..13579)
                     /gene="folE"
                     /locus_tag="PLW95_06140"
     CDS             complement(13001..13579)
                     /gene="folE"
                     /locus_tag="PLW95_06140"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOV22243.1"
                     /translation="MMDRRKIEKGVELILEGAGIDTNKNSLKETPKRVAKMYEEILSG
                     MGQDPEKIIGKFFYEEFNELVLVKDIPFYSLCEHHLLPFFGKVHIAYLPDGKKVVGIS
                     KLPRLVDVFTKRLQLQERMTNQIADTIMKVLTPQGSMVVVEGEHLCMVMRGIKKPGSK
                     IVTSAMRGIFLRDLRTRGEALNLIMAGRPSSI"
     gene            complement(13533..>14649)
                     /locus_tag="PLW95_06145"
     CDS             complement(13533..>14649)
                     /locus_tag="PLW95_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOV22244.1"
                     /translation="PCIIFIDEIDAVGRQRFAGLGGGHDEREQTLNQLLVEMDGFQTD
                     EHIIVMAATNRPDVLDPALTRPGRFDRTVVVDMPDIRARELIFKVHTKNKPLDETVDL
                     KILARSTAGLSGADIANIVNESALIAARKGKEKIDMNDFEEARDKVLMGTERKNLVIS
                     DEERKIIAYHEAGHTLLQKSLPDVYPVHKVTIIPRGRALGLTHILPEQDKFIQSDIYY
                     KNSLCTLLAGRATEKLIFGKMFTGSENDLKVATEIARRMVCEWGMSEKLGPVIFRHDG
                     NVFLGRDLVQMREHSEVTTREIDEEIKRILEEAENKAINILTENLDKLHKLANALLEK
                     ETLKSEEIDEILGIEKQEQKGENDGQKENREGSGTDS"
BASE COUNT         5133 a         2135 c         2169 g         5212 t
ORIGIN      
        1 ataataccag ttgaaactgt aaaagatgtc cttgaaaaag cattaatcca ataaatatga
       61 caaaacaacc atataaagaa ttttttggaa agatttaaaa cactgataag taaaaatcct
      121 catcttatta aaattacttt gagtaatatt tttacaatgc gacttataag aaataaaata
      181 catggagatt tagaggaaat agccctcgca gaatttatta atcaatatat gtatgactat
      241 aaatctgaac atattggaaa gcaaaaattc tgagcgaaag aatttgaaga agatataaaa
      301 ataattaatg agattaacag agaggaattt ttaataagta taaaggcata tggatatgga
      361 ccgcttcaat tatcaacaga taaagagttt aaaatgttcc ctgcttttca aaaggttttt
      421 ggaacagaaa atcaattgaa gggaacgata aaattttgca gattttagaa agcgaagaat
      481 tttcaggatt taatgattta aatattctgc ctttaatcta tgatgaaaaa aataaaaaat
      541 gtaacataat ggtttttgat ttagataaag caaaaaaaga gagggaatat aagaataaat
      601 tttgggaaat atttggataa aaggaggtag gaaaatgcga aaggtaattt gtttagttgg
      661 ttttgtattg ttattctttg gaggattgca tagagaatat attctttggg gttctgaaga
      721 gaatatcaag gagattctca ccatggaaga atggaataga taccaggaat taacaggaat
      781 acaatccaaa aaaaaggagg tcagtggaaa gtactggcag gcagtagggc caagttttga
      841 tgttgcgaac ccaaaagata aagagccagc acaactttac aaggaagaac ttgagaaata
      901 cgacaagata cttaaggaga aatcgagaga gttttttgaa attcttattc cacaaaaaga
      961 aaagatattt ttgcttgcag tcgagataga tataaaagac tctgaatcgg ccggttggat
     1021 ccttgctaaa ggtttttcgg atatagaaag taatcctgaa acatttttga aagcatatgt
     1081 ttgtttaaat aagtatgaag acagaatgca gagaaaagag atgcgagatt caatgagaaa
     1141 tggaattttt tatctggtag ataaatctac ttttgaacag gaagaaaata gaattagatt
     1201 tgccaataat tttcttctgt cagagggttc aattgaggat aaaaaaatta ctgcgaagtt
     1261 cctctgggaa gtatggagcg gatttttccc acggatagaa aggatggaga acaagagcga
     1321 agcatacgag aagatgaatc agcaatcctt gtcaatcata gagacattga actccatcaa
     1381 gacagatata aaattgacag aggaagttaa taaggttctt cagaatttcc atgcacatta
     1441 ttcaaggaaa gatttgcctg tggaattcct gcaaagttct tcaatatcca atgtcaagga
     1501 aaagatcgct caatctatga aaaaggatta aaaccttaaa aaaaatggga agggtaatat
     1561 acgatgggaa aaagaagaat gggaaagatt aagagaatat ttaccgtttg tatagttatt
     1621 gggatactgg gaagcattct ctttcatgtg gttggtatta ccggtaacat ttccgctgac
     1681 aagattagaa aaactgagaa ttttcaaaca aaagaaagga ctactttaaa taatgttcag
     1741 taatatcagc ccttgaatgc cccatttttt cagatgtttc attaagggat tgttcataag
     1801 tcataccatt ttccatattt tcctgatgtt gttcttgtgc ataattccat ctcaacccgt
     1861 gagtcccgta atagtcctcc tgtaattgtt ggcatgtttc cttcaaatct tctctcctaa
     1921 aatcccgttt tcttataact atggataagg agaaaattat caaaaacaag gagtagtgtg
     1981 gatttaggat tgtgaatttg ggtatagatt ttgaaagggg ttaatactat ttttacatca
     2041 aaggtgtaat aaaaatcagc ctcctcacct atatccctcg ggcgtagtcc gaaggctcgc
     2101 ccgttgggct atgcccagag ggatccgtaa gcaaaaagcg aggaccctac gggcgagcct
     2161 tctccccatt gggtagaagg aactaccccc ctcacccctt ccctctcccc ttgggggaga
     2221 ggataggtga ggggggaata tttactctat gtatgttgtc aaattggtat taactgcata
     2281 gttattaaaa tttgcgggat tttcagttat ttttattaga gaagtgaatt cttaacaata
     2341 gaaaacagaa gtagacagag gaattgttgc aaagcattca atttcaatta accatccagg
     2401 acgacataca gaagcataac agataattag aggagtattt aaaaattttt ctctaatttt
     2461 ctttttaacg attgtataat cagatgggtc tctaatgtaa acaaccaaaa gtgatatatc
     2521 acttaattta gcatttgctt tttttaaaag tgcatgaaca ttttcaagac atctttctaa
     2581 ttgctttgta atatttccgt agtataaaat ttctcctttg ttatttatac ttgcagttcc
     2641 agatattata atatgtttcc tgtctttata agaaattgct actcctcttt caaatgtaac
     2701 tccataaaga taagttggtc ctaaatattc cggtgcagat agatattcaa cctgttcttt
     2761 tgaaatacct aatattgaat atgcatccat aacaactttt acatcagagt caaaatattt
     2821 cccttcaaca cctgtacttg caataaaatg tgtatctttt gttaatccat tttgagcata
     2881 aaattctctt cttgcatcaa caaattcttt ataatttaaa tcaatattct gtataaaaaa
     2941 ccatgttcta ataacattat ttaaaagtgt cattttattt tttaaaagaa aattattata
     3001 tttttttaga actccagttg tttgttcata aactgttttc ccttctggat aagttatacc
     3061 agttgtcaaa ttatgaataa tatttcccct tttgaaagaa aaattattat tttttaactt
     3121 cttctttgtt ttatttaatt tatcctttat atgatatgac cataaagcaa cttttccaca
     3181 aggacctggt ggttgacaga tatatgaaag accacatttt gagaaattga ataaattttt
     3241 attttttaaa taaattgcct gattgaataa atcactgcaa taaaagcgct tgaaaaccga
     3301 tgtttcttca tctaatccaa tatattttaa tgtttcttta tatgcagtat atacccactt
     3361 aatctggttt tcaaaattcc catattcttt tggtataaaa actaaatggt attcatcagt
     3421 accagattta ccacaaaaat gtgagaaatt tattattaca ggaatttttg aaatttcata
     3481 agaataattt tccataattt tattttaaat aatttcaaaa ctaatagcag gtaaaaagcc
     3541 aatagaaacc ttttctactt tttgaactgg tttttcagga gtagaagttt tattttctct
     3601 aaagacctca ataataagat tatcagcaaa tcctgacttt atttctttac catttgttct
     3661 taaattgaaa cttaaaatcc cattttcaat tttcacatct tcaattgaaa tttcttttgg
     3721 tggttccttt aattctaatg atatttcatc acttttgagt ttttgtaatg tctttacttt
     3781 gacattgaca acaccatcta ttggaatttt taaaatatct tcatcaatca atttaaagtc
     3841 aaattgagaa atttttcctc tatttataac ataacaaaaa tttttttcaa aaggaactaa
     3901 atgaaaatag gcaaatgcct gcatcatttc ttctgctggt atagcaattc ttttaacttc
     3961 tttcccctca ataattgtac taccttctat ttgaatatca aatgggtttt cataaggtgc
     4021 ttttaaagat gaaattgtta ttcttacatt atcttttcct tttggaattc ttcccccatt
     4081 caaaataaag ttaggattag gttcttttaa tgaaattttt atttccccat caaatccatc
     4141 tttcctgata gcatagatat ttaagatatt atttctaccc gccaaaatgt ttatacttga
     4201 aggaataaca aatagtttaa aatcaggttc tggattttca atacgaatat aataaagata
     4261 attatctcct ccatggtttt gaatatctaa aatctgtaaa taataatatc catcctttgg
     4321 aactacaaaa gaagtgtatg aatctgcatt atgagtgata agtccaaaac ttttatcttc
     4381 ataatcatca ttaaatttta atgtttttcc agaattatct aaaatacgga taaaagaatc
     4441 taatggataa ccaagtcgtc tactataaac atcaaaaata atttcttccc ctatttcccc
     4501 tttaaactta taagtatcaa tatcccctgg tttttcaata atacctttta taatttgagg
     4561 taatattata taatttgctt tatttaactt atcatttggc tctttttcac aaatttctgg
     4621 caatttattt aaaatatcgg aatttataac atttttaatt tcttgaaaat tatttttaaa
     4681 tgcacttttt tctcctataa ttaatctgta aacaaagtcc tctcttccac gaaaaagagc
     4741 atctctgact tctattatgt attctccatc ctcttcaact tcataaaaaa ttatagggtc
     4801 tggattaaaa taataattat cagcataaaa tatttctttt ccttttgatt tataaagagt
     4861 taaaatacct tgaaaccatc caggaacagc atctgccata taaggaataa tatctcttgc
     4921 ttttaattca ataaaaagga attgtccttt ttttgcatta aaccaaaatc tatcaacatc
     4981 accgggcata attctaccat ttacaataat tggtaaatca agaatatttt tctgttgttc
     5041 tattggacca tttggttctt tttcgtttat ttcattaaag gtatttactt caaaaaaaag
     5101 tggatttgta agtcctcttt gtgttaataa tcttatttca tattttttag gttcaacatc
     5161 agaatcaact ttcaaataaa ttagaacttt ttcttgaata gaccttttta tttgaactct
     5221 ttgaattggc attaaaaaaa tttcaaaaat tttatctaat tcttctggac ttaaattttc
     5281 cagatttttt aaaagaggat tatctggaag ttcaacaatt tcatcttgac ttaatttttt
     5341 ttcctggtag atacctttgt tttttctctt taattcagaa atttttttta aaagataatt
     5401 tctttgcatg ttgtttaata caggaatata aataattgtt tctgccatta ttccatctga
     5461 ggaacaacat atatctttaa cacctcttaa attttgccct ccaactaata tctgtacaga
     5521 acttcctttt ttgcctccag aaggataaat ataaccaatt gatggttgag atacactttg
     5581 tgctattaag gtaaaaccaa aaatgaaaat aaaaataaaa attgttttaa ttcccttcat
     5641 ttattatctc cttcaaaatt tcctttccag ttcctttttc aattggtggt agaactctta
     5701 tttccccttt acctatatca gggaaaattc cttctgggtc aatccccaat aaataataaa
     5761 tagtcccaag taaatccttt ggataaacag gtctttcaat aacatattct cctttttcat
     5821 cactttttcc caagacaata ccacctttaa aaccacctcc agcaactaaa acagaaaaac
     5881 aatttccata atgaccacga ccaccattcc atggtggttc ccatgatatt tttggcgttc
     5941 ttccaaattc accagaaacc catataattg tattttcaag tagtcctctt tcagaaaggt
     6001 cttttattaa tgcggaaagt ccctggtcaa gttctggtaa tttttgtctc atctgttgga
     6061 aatggttact atgtgtatcc catccaggat agtttattgt tacatatatt actccgtttt
     6121 caataagttt acgcgccatg agacatgatt gcccaaactt atttcttcca tacatatttc
     6181 ttaaatcttc tggttctttt gatatgtcaa aaattttttt acattcattt acaattttat
     6241 atgcttcttc tttacaagaa tcaaaagatt gaaactcttc aagagatgca attgaaccaa
     6301 gtgtatctaa tgaattaaga agttttgttc ttcttaaatt tctttcctca gatatccctt
     6361 caacaacaat tccctcaaca gcaaaaggtg ttctattagg gtcccctcct gtaacaaatg
     6421 gtttatattt ttgtcctaaa aaaccttctt cagaaaaacg tccctgagaa gttgttaaaa
     6481 caatatatgg tggaataatt cctttatatt cttttccttt aaaaaatgta actgctgaac
     6541 caatacttgg atataaaacc ctttcatcag aagaatgtcc tgtttgcata atataagatg
     6601 ctgtttcgtg ggcagtaata ccatgtgtta tacttcttat aattgaatat ttatcggcaa
     6661 ttttagcaag atttggtaat aactcattta tttctattcc tggaacattc gtaggtattg
     6721 cttgaaatgg tccagtgtaa tcataccctg catttggttt tgggtcaaaa gtatcaagat
     6781 gtgatggccc tccccataac catatctgaa taactgcctt tgctttttta tcaaatgatt
     6841 tttcctcttc tccaaatccc attaaagtaa atagaacatt aaaaactaaa aaactaataa
     6901 atattttatt tttcatttta attttcaatg attgtataaa aattccttac tattaataat
     6961 tgcccaaaca atatcttcta ctccctcatt taaattaact tttccatctt gaaaatattt
     7021 ttccaatgta tcaatttctg attgttctgg gtatcttgaa agtaaaaata aatatatatt
     7081 tctaattatt tcttttctat ttccttttgt ttcccttata attcttttta aatatggact
     7141 actttttatt ttcttttgta tttctgatga attcaaaaga tatctaattt ggacttctgt
     7201 aattttattt tctctttcag aaaaaagtcc tgtatttctt gatggtcttc cgaacatttc
     7261 aagaaatgga cttgtaatac ttccatctgc aagaagaaca tttttttgat attttggaat
     7321 aaaagtatat ggttcaggaa tttgactgat atattctact ccatctcctc ctatattaca
     7381 caagatatct aataaaacct cagcatcaat tcttttgatt tgataatacg caaaatattt
     7441 ttttgcctct gggttatttg tttttggaat aaatgattgc tgatatgttc ttgaattcaa
     7501 tataagacga taaatatgtt ttaaatcata atttgaatta acaagttgct tttcaagata
     7561 aataataagt tcgggatttg atggaggatt atcaggtctt atatcatcaa cttcatttat
     7621 aattcctgtt cctaaaagat atgcccaaat tctattaact atatttttgc aaaaccattg
     7681 atttttcgga tttattaaac agtcagcaaa tacaattctt gggtcaattc ctgaaggaat
     7741 tattactttt tcaccatcag gaaaagtgca ttgtattgtt tcagatggtt caggattaaa
     7801 atatacaatt tcttctttcc attcaagtgt attcttataa gaaactcttg aaaaaaactt
     7861 actcatttca tcttttgttt tttggggtaa tttatcaaat cttacaccca taaaagtcaa
     7921 tgcgaccgca gaagttatag aatatgggtc tcttccctgt attgctctat aaaaatttac
     7981 aggtggtgta tagaaattac taccacttga agtaagtaat tctcttacaa acttatcata
     8041 atgtttgttt tcttttaaag aatttaatat ccatttatga tatgattgaa ctgcatttgg
     8101 ccagaggtta attggaaatt ctgattttac tcttaaaata tcacaccatt tcattgacca
     8161 atagtcagca aagtcatctt tttcaagaag agcatcaata agcaaacttc ttttatctgg
     8221 gtttttatca tttaggaaag aaataacttc ttgattttct ggtaattttc caattagatc
     8281 aagatatacc cttcttataa atacctcatc agaacactct aatgcaggtt caattccctg
     8341 tttttttaaa ttggaaaaaa catacttatc aatctctgtt tcaggaatat cccatgaatt
     8401 tttctcataa gtggaaatga ttttttctga ataaaacaaa ttattttctt tttgagcaaa
     8461 acacccattt acaataaaac atattaagag aaaacatata attttaactt tcatatattt
     8521 atatttgaca taaataatta taaaatagtt taggcaattt aaaaaattaa aaaatatgat
     8581 aaaatattat taaaatttca tttttaaaaa cataatgttt ccaaacaaaa ttttttgatt
     8641 tttgataatg gaggggagag atgaaaaaga aaagattgtt tacgccggga ccaacttctg
     8701 tcccggaaga aatacttctt gaaatggcaa agccaataat gcatcatagg acagatgagt
     8761 tcagagcaat agtaaaagat gtttctaatg gattaaaata tatttttcag acagaaaata
     8821 gcgtttttat tatcggttca tcaggaacag gagcgatgga aacagcaatt acaaatgttt
     8881 tctcaaaagg agaaaaaatt gctgttgttt gttgtggaaa atttggagaa aggtttgcag
     8941 aaattggaaa tgtctacgga cttaaaatat tgccggttga aattgaatgg ggaaaaacat
     9001 taaaagcaga agaacttgaa aaattactac gagaaaatcc agatattaga ggtatttgca
     9061 ctcctttatg tgagacatca acagggactg cttttgacat agaaaattat ggtaaagtta
     9121 tttcaaaatt tcctgatgtt attttaattg ttgatggggt aagcagtgta ggagcaattc
     9181 cctgttatat ggacaaatgg ggaatagatg ttcttattac tggctctcaa aaagcactta
     9241 tgcttccacc gggtctttct tttattgcat taagtaaaaa agcatgggga aaaactgaaa
     9301 attcagatat tccaaaatat tattttgatt tgaaaaaata taaaaaagca attgaaaaag
     9361 atgattttcc ctttacaata cctgtttcac ttgttattgg tttgaaaagg tcagttgagt
     9421 tgtttcgtga atatggactt gaaaatctat ggtcattaca ttcaaaaaga gcaaatgcaa
     9481 caagaaaagg gattgaaaaa atgagattgg aacttctgtc tgaaaatcca tctgatgcag
     9541 ttacagcaat aaaattacca gaagaaattg atggagagaa attggtaaaa aatttaagga
     9601 ctaaatatgg aatatcagta gcaggtggac aggacaagct taaagggaaa attataagaa
     9661 tttctcattt aggatatatg gatgaatttg atgttttaac agcaataatt gctgttggaa
     9721 tatgccttga ggaaatgggt tataaatgca atttagagga tggtataaga gaagcaaaac
     9781 aggtactttt ttcataaatt ttttctccag ttataaaatg gaaaaagaag ttgaaaaaat
     9841 aataaaaaag gcaataaaag aggatttagg gaaaggtgat gttacaacct ctgctatttt
     9901 caaagagaaa tttcctgtaa aggcaattct tatttcaaaa ggtgatggaa ttttatgtgg
     9961 tgtggatatt tttaaaaatg tctttaacat tctttcttct tccttttctt ttaatttttc
    10021 cttcaaagat ggagataaaa taaaaaagaa agatatagtt ggagaaattt acggacctat
    10081 aaaagagtta ttgataggtg aaagaactgc tttaaatttt cttcaacatc tatctggaat
    10141 tgcaactgaa acaagaaaac ttgtagaaag ggcaaaaaat aaagtaaaaa tatacgatac
    10201 aagaaaaaca ataccaaatt taaggttatt ggaaaaatat gcagtaaaaa taggtggagg
    10261 agaaaatcat cgttttggtc tttttgatat ggttcttata aaagataatc atataaaagg
    10321 agtaatggaa aaagagaaag ttgataaaat ttccgcaatt tcaatttgtg tcaaaagagc
    10381 aaaaaattac tggaaaaaca aaataaaaat agaggtggaa gtagaaaatt tcaaacaggc
    10441 aataagtgct taccaagcag gagcagatat tataatgttt gataatgcgg ataaaaatca
    10501 actgcttgaa tttaataaat ttttaaaagg aaaaaagaaa gttgaaattg aatggagtgg
    10561 aaatgtttct ttaaaaaaaa ttgataaaat aaaaaaattg cctgttgata gggtctctgc
    10621 tggttatatt actcattctg caaaagttct tgatttctcc ttaaaaatct cctctattta
    10681 gtttctgtta gagaattctg aaaggtgtaa attattgagg ttcaaacact tattaacttc
    10741 gtaagttaac aaagggaaat aaatgaaata ttttgataca cattgccatc ttgacttctc
    10801 tgattatgat agtgataggg acaaagtaat agaaaaaagt aaaaatgaag ggattgaata
    10861 tataataaat gtaggtgtta atcttgaaag tagcaaaaaa tcaattaaaa tagccagcag
    10921 gtataaattt atttttgttg gtgttggtat tcatccttct gatgtgtatc aatgtaataa
    10981 aaaagatatt tttgaaatag aaaaattgtc aaaagagaaa aaagttgtgg caataggaga
    11041 aattggactt gatttttatt acaataagga taatagagaa aaacaaacag aattttttat
    11101 aactcaatta aaaattgcct ctgaaaattc attacctgtt attatccatc aaagggaaag
    11161 caaagaagaa ataattgaaa ttttagaaaa aatgaataaa aatttacctg aaaaaattgt
    11221 ttttcattgt tttggagaag ataatgtttt tgctaaatat tgtaaagaaa aaaatttcta
    11281 tatctccttt acagggatta taacttttaa aaatgctaat ggaataagag aaattgcaaa
    11341 ggactttcca ttagaaaaaa ttatgtcaga aacggatagt ccttatcttt caccgactcc
    11401 ttttagagga aaaagaaatg aaccatcaaa tgttagatat attgttgagg aaattgccaa
    11461 tcaaaaaaaa caatccattg aagaagtttc taatgttatt tttaaaaatt ctgttacttt
    11521 tttctctatc ccatcttact gagatttagc gaataatagg aattatgata ttattttatt
    11581 aacttaccag gttttaataa ttttatgtca gttttggtta tttttatata tattggagaa
    11641 tattttatgg aatttattac ttttggatgt aaagtaaatc aatatgagac acaacttcta
    11701 aaagagaatt ttccttatat tgatataatg cccgataatg tttgcgtaat aaattcttgt
    11761 tgtgttacaa ataaagttga aaaagaggtt aaaacagtta taaggaaaaa gttaaaaaaa
    11821 ggtaaaaatg tatggttaac tggttgtttt gttaatgaag aaaaaatcaa attgttgttt
    11881 ccttctatta aaatttttaa taaatttgaa ttttgtaaaa aagtaagaat aataaaatca
    11941 tttaataatc atacaagagg ttttgttaaa attgaagatg gttgtgaaaa taattgttcc
    12001 tattgtataa tccctcttgt cagaggaaaa gttaaatcaa gagatgaaga agaaattatt
    12061 gatgaaattg agaaattaac tgaaaatggt tataaagaaa ttgttcttac tggaattgat
    12121 ttaggtgctt atggaaaaga taatggtaaa aaaattacta ctctaattga aaaaatctca
    12181 caggtaaaag atttgaaaag gataaggtta agttcaatag aagcagttta tataaatgaa
    12241 acactaattg actttctttc ttctcttgat attttctgcc ctcattttca tattccactt
    12301 caaagcggtt ctgataaaat tttaaggtta atgaaaagaa attataactt atctcaatat
    12361 ttaaaaaaaa ttgaagttat aaaagaaaaa attagaaatg tcacatttac tactgatata
    12421 atagttggtt ttcctggcga agaagaagat gattttaaac agacatgtaa ggcaattggg
    12481 gaaattaact ttttgaaagt ccatattttc cctttttcac caagagaggg cacaaaggcg
    12541 tattttatgg gaaataaagt tgattataaa gttaaaaaag aaagagaaaa agttctatct
    12601 tattcagtta aaaaagaaag agaaaaagtt atgaagaaat ttctttttag ctctctttct
    12661 gttctttttg aaaaaaaaga agaaaattta tggactggct attcagaaaa ttacattcca
    12721 ttttcagtta tttctgatga agatattaaa aatgaaatag taaaagttat tgggaaagaa
    12781 attagaaacg ataagattta tggtataatt taaaatatgg gaaaaaatgg aaaaatttga
    12841 aatagtgaat ttagtttcag gggtattact aattgtatta ggatgctttt tcacatttaa
    12901 accaaaactc acatatcaaa aagaaggcat ctctgatatt tttgcggtat ttggaattat
    12961 tcttatggtt atcggagtag ttttactttt ttctcctctc ttaaatagat gatggtcttc
    13021 ctgccataat aaggttaagt gcttcacctc ttgttcttaa atcccttaaa aatattcctc
    13081 tcattgcaga tgtaactatt ttacttcctg gttttttaat tcctctcatt accatacata
    13141 aatgttcacc ttcaacaaca accatagaac cctgaggagt cagtactttc attattgtat
    13201 ctgcaatttg atttgtcatt ctttcttgta gttgcagtcg ttttgtaaaa acatcaacaa
    13261 gacgaggtaa tttacttata ccaacgactt ttttaccatc gggtaaataa gcaatatgaa
    13321 ctttaccaaa aaaaggtaga agatgatgtt cacataaaga ataaaatgga atatctttca
    13381 caagaactaa ttcattaaat tcttcataga aaaattttcc aatgattttt tccgggtcct
    13441 gtcccattcc acttaaaatt tcttcataca tttttgcaac tctttttggt gtttctttca
    13501 atgagttttt atttgtatca attccagcgc cctcaagaat cagttccact cccttctcta
    13561 ttttccttct gtccatcatt ttctcctttt tgttcctgct tttcaatacc aagtatttca
    13621 tctatttctt ctgattttaa tgtttctttt tctaaaagtg catttgccaa tttatgaagt
    13681 ttgtctaaat tttcagttaa aatgttaatt gctttattct ctgcttcttc aagtatcctt
    13741 ttaatttctt catcaatttc tcttgttgta acttcactat gttccctcat ctggacaagg
    13801 tctctaccaa gaaaaacatt tccatcatgt ctgaaaataa caggacctaa tttttcgctc
    13861 ataccccatt cacataccat ccttcttgct atttctgttg caactttaag gtcattttca
    13921 ctcccagtga acattttccc aaaaattagt ttttcagttg ctctacctgc taatagtgta
    13981 cataaagagt ttttataata aatatccgat tgaataaatt tatcctgttc aggcaaaata
    14041 tgagtaagtc ctaatgctct tcctcttggg attattgtaa ctttatggac aggatagaca
    14101 tctggtaaac ttttctgtaa aagtgtatgt cctgcctcat gataagcaat tatttttctt
    14161 tcttcatcac ttattacaag attttttctt tctgtaccca ttagaacttt atctcttgct
    14221 tcttcaaaat cattcatatc aattttttct tttcctttcc ttgctgctat taaagcagat
    14281 tcattcacta tatttgcaat atctgctcct gaaagtccag cagttgacct ggcaagaatt
    14341 ttaaggtcaa ctgtctcatc aagtggctta ttttttgtat ggactttaaa aattagttct
    14401 cttgctctta tatcaggcat atcaactaca actgttctat caaatctacc tggtctggta
    14461 agagcagggt caagaacatc tggtctattg gttgcagcca taactattat atgttcatct
    14521 gtttgaaaac catccatttc aacaagtaat tgatttaaag tttgttctct ctcatcatgt
    14581 cctccgccaa gcccggcaaa acgttgtcta cctactgcat ctatttcatc aataaaaata
    14641 atacatgga
//
LOCUS       DAOOVW010000040        14619 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_2747_length_14619_cov_7.516822, whole genome shotgun sequence.
ACCESSION   DAOOVW010000040 DAOOVW010000000
VERSION     DAOOVW010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14619
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2747_length_14619_cov_7.516822"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..2074
                     /locus_tag="PLW95_06150"
     CDS             176..2074
                     /locus_tag="PLW95_06150"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOV22245.1"
                     /translation="MIKLYVSQEGNDNWSGQIPFSNQNNSDGPFRTIQRAKEEITKQK
                     ENGKLRQPIVFYMKRGTYFLDEPIIFNSDDSGTPKFPVLFTSFKNDKVVISGGKKIEK
                     WNETKINGKEIIVFKVPFIKDDLREIFINGEKRNRTRFPEKKFLLYKRDKIPSDWNKG
                     EDLIYLREDILGNIKYIENSEFIFLHLWVDVHLPVKEVDYEKGIVKFKKKSLRYLYDG
                     DTPARFYIENIFDIDQKGSFYFDKEKKEIYYIPLENENIFEIVVPYLENLIIFEGNYK
                     NNEFVEYINFKNIIFSHTNFTLDKNSAGDMQAGYEISGSIKGSGCKNITFENCNFSKL
                     GNYAIEFRDGCKNNEITKCKIFENAGGGIKIGEFLRSDEVDLLKNDYLRHTYGNKIVN
                     CEIYNCGKTFHQCVGVLIGQSYNNIVSNCNIYNLYYSGISVGWTWGYKTSLAYNNIIE
                     YNQIHHIGKPEREKEAFLSDMGGIYTLGIQPGTILRKNKIYNISAYRYGGWGIYLDEG
                     SSEIIVYGNMVFYTTHGGFHQHYGKNNLIIKNIFAFGKETQIVRSKEEQHLSFKFVDN
                     IVYWEEGELLKGNLKDFNFYFDKNIYWNTSRQKFKFGEFTFEQWQEKGMDKNSKIEEV
                     DISLFLKK"
     gene            complement(2117..4252)
                     /locus_tag="PLW95_06155"
     CDS             complement(2117..4252)
                     /locus_tag="PLW95_06155"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOV22246.1"
                     /translation="MKKEKFMILGVQYYRPPFPEKKFWKEDLSKIRDSGFNTIQLWAC
                     WGWIEPKMGIFKFDDYDELISEGEKKGLNVIISTIAEIHPFWIHRIIPDSYMIDHMGN
                     NVISSLRIECNVGLTPGGCTDHLKVQELMENFLKTIAKRYAGVKNLIGWDCWNETRWA
                     VQADGYVCYCPNTLNLFREWLKENYESLENLNKAWKRRYSQWEDVFPGKLPARPYTEM
                     MEFLKFLTWRATKHMKFRYESIRSEDKEHIITAHCGQPSIMSLEGGFEQTLCRGNDWF
                     LADQLDGFGSSHFPLWGRFDESILGVRIEAIRSATRGKVMWVSELQGGSARNGHMVDL
                     SVPADLQQRWIWNGYGRGAKAVIFWCWRDEVFGRESSGFGIAGNDGKAEERIKAMKKT
                     GKLLNKYNDLFQTYQPDNPKVGVLFEPDNYYLNWASDGNSELAGKNLLGYLLSLENCN
                     IPYEIVESNHLDILYKLKVLFMPWSLIIRPEVAVHLMKFLQSGGTIFCEGETDSFTSL
                     GFYRYPGEERNFTWELGISDEGRRIINQEEIAVFLKNKKFSLKPSGWLTPLVSKNSSF
                     ILARDINNNPIFISQNFGKGKVYMIGTFIGLPYYCSQYKDFEELLFEVVKDCNGLPEL
                     TVESKEETNTIKWQTGISGKYRLLFLTNNGNEKSVFISASEKFWGNSSEIEDLCTNEK
                     IPIEIKNNKKGFKKDIAQGIFSIFRWEKF"
     gene            4475..4627
                     /locus_tag="PLW95_06160"
     CDS             4475..4627
                     /locus_tag="PLW95_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22247.1"
                     /translation="MLVDEEIILEGQITQQKYPEKLRIITYYDDEHDKIYQFLTNNFY
                     RFLSRE"
     gene            4656..7247
                     /locus_tag="PLW95_06165"
     CDS             4656..7247
                     /locus_tag="PLW95_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22248.1"
                     /translation="MLKKILTRLVFGLFLLCFAGNVLGLEMKKYIYTEDFEEKDPVKF
                     WTSNGKYTINFKGLTDEKAFSGKQSFKLDITFEEGNFFYWCIPVKVPAEGELKFSGSI
                     LLGEETTGSAGLGVNVIFPPTWYSSCGSFDTYGTTKGEWKLIEGDLVKYGKETAEGVF
                     KQHVWWVSGKDVGVYVDRVGIFLYGSSGKRVVVYVDDIKIEGEVPTEESYKQEIERRW
                     SVVKTRFDEKKISDWEKILVESQQEISTFTSLTPEAEVIKKDVEEKINILKKEIEEIK
                     KRGYINKPEVDEMNLSLTQLKDTIKTIKSISEREIKQRGCLIYIISPISGQKILPKDT
                     FIPGNISDEIQLVATPGEYESGSFVIHSLSNITSLKVEVEDLKGEKGIISSENIDIKL
                     VKCWYQSGTAWSSIIQEKNNRVLVPELLLNDDNLIKIDYENEKNYLKLNFPEGEKYIW
                     ISDPDEPEGNQAKILSINDYPVKDSSVLLPVNIPEKTNKQFWLTIKVPSDTKDGIYTG
                     TIKLSDTKGEIGEIILRVKVLPFILASPKTYYDINEDFISSIYYRGVLHKDYPEGTIS
                     SEYKSKEQLKAELKDMYEHGITNPICYQGSDEKFLGEYLKIRQEVGIVNQILYSIISA
                     NCPPSAIKKIIEFVKEYGIEEVYFYGIDEASGEMLSAQRPAWEAIHGAGGKVFVAGYK
                     GDNFALMGDIQDLFVRAGYPSREEAEKWHAVGHKIWCYAHPQAGPENPEVWRRNFGLV
                     LWKSNYDGAATYAYQHSFGNIWNDFDHPTYRDHNFTYPTVNGVIDTIALEGYREGQDD
                     IRYGTTLKIEIEKGKKSKNQKIKSIALEAEKYLEEIDVERGDLETIRLEMINYILKLT
                     DRYEKQR"
     gene            7281..8153
                     /locus_tag="PLW95_06170"
     CDS             7281..8153
                     /locus_tag="PLW95_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22249.1"
                     /translation="MRKFDYNKIKNPVMKDKEVNLRDPACYLKDGIIYVFFTIHNLKD
                     DTVCIGMTKTYDFIEFDKIRSISPIGYASPGNIIKVENKWFLCYQQYKEFPHYLCYSW
                     TYDFENFSPPKKFFNTDKTNKWNEDGRTIDPYIVENNGVYYCFYVGSNRWLKGNGYNM
                     IGMAKSYDFENWQDVSKEKPVIGVNYDWEKPDGNENNCVIKVGEKWVMLYSASLTYQK
                     IAYAESYDLLNWNKRCLCDVPIIKESELRFGAPFIIEGLSKNSEYYMIYMGEDKNNRT
                     SFILLKSYDLIHWS"
     gene            8246..9754
                     /locus_tag="PLW95_06175"
     CDS             8246..9754
                     /locus_tag="PLW95_06175"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HOV22250.1"
                     /translation="MGVKKYLAIDLGAESGRGIVGEFNGEKIKIKEIHRFPTYNTNVF
                     SHIFWDVLKIFDEIKIILKKTKEEGEISGVGVTTWGVDCAYIGGGNLLLSNPFHYRDS
                     RTDGIMEEVFSIIPKEEIFEKTGIQFMPINTLYQLYSTKKEFPYIINSTEKLLFMPDL
                     FNFFLTGKKFSEYTIATTSQMYNSLLEGQDKAPRGNWAYDIIEKLNIPVSILPEVISP
                     GTFLGKMEKGISEEIGLEDINIYAVCSHDTSSAVVSIPAKEEKYAYISSGTWSLLGVE
                     LFQPIINDKSFNYNFTNEGGFGGSITFLKNIMGLWILQECRRYWEKEGNYYDYPTLTE
                     IAINSQPFYSFIDVNDGVFLFPGNMPEKIKEYCKKTGQKIPEEIGQIVRVILESLALE
                     YRYNIENLEQIIEEKIDVVHIVGGGSQNKLLSQFSASSMKKIVITGPSEATGIGNLLV
                     QAIGDNEISDVSQLREIVRNSFPLIVFQPRDTSNWEESYEKYKKLKEFHDGL"
     gene            9744..10382
                     /locus_tag="PLW95_06180"
     CDS             9744..10382
                     /locus_tag="PLW95_06180"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HOV22251.1"
                     /translation="MDCNFLSDSLITYLEKISSKTSIPGGGSVVSLVSSLSCSLLDMV
                     LNYTIGKEKYKKYENKFIDIKNKNQKIKKVLYSYIEEDSKIYKKIKENSKNEEKQQIY
                     LEKSIQMHNEICNYMADLIAFSFFVAKYGNKHLISDSGIVAILSLSSFKGAKLNILIN
                     IKYIDENGKNKYLPLMKILNKKEKELEKKVEIIYNYAVEKIGVQNGRYYRWK"
     gene            10357..11220
                     /locus_tag="PLW95_06185"
     CDS             10357..11220
                     /locus_tag="PLW95_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015902602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HOV22252.1"
                     /translation="MGDIIDGNKIAQRITDNTKKEIEELKEKGIYPKLVAVSVGENPA
                     SSVYIKQQKKNCEKIGIEYELKKLPESYNENQIISFIEELNLDNKITGIILQLPLPSG
                     IDVKNIQSKISPIKDVEGVNPVNLGWIIYGKPFLAPCTALAVKKIIDSLNIDLYGKEV
                     VMVGHSDIVGKPVALLLVNEFATTCICHIGTSDKGNLETHIRRAEILIVAVGKANLIP
                     GDWVKEGSIVIDIGINRVGDRIVGDVEYEKAKEKASLITPVPGGVGPVTTAILLRNIV
                     MAAKSTVNLRS"
     gene            11811..12605
                     /locus_tag="PLW95_06190"
     CDS             11811..12605
                     /locus_tag="PLW95_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22253.1"
                     /translation="MIILIIGLIIGLIIIAVEIYFVVKAKRKENMAEEKKKKIEETAE
                     IIKNKAISEAEEKLKGANEKLENIKREMAEIQQEKNRSKKERETIEKIKEELKEEKKS
                     LNKKEFILIQKEYELEKKEQDLSFQKKMNDEEFIRQEENLNKLRARIKEESLKVDETK
                     AEIREKIMLLQEQEKSLAMKDKESKKIEENLREQQRELIKREKWVISKEKELNEKIGE
                     YEKKLEEIAEISKKDAIDILLKKMENEAQKEFLEKGKLRSWDDFLK"
     gene            12621..14462
                     /locus_tag="PLW95_06195"
     CDS             12621..14462
                     /locus_tag="PLW95_06195"
                     /inference="COORDINATES: protein motif:HMM:NF024733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system Lon protease-like protein BrxL"
                     /protein_id="HOV22254.1"
                     /translation="MQLDEKLKNLFPNLVVNKKLALTKEVSRLPRFISEYLITKFLEN
                     DKLNTEKLYNFLKEHYISPEERNDALHQLRKEGIKIIMDEFYVEMKVSGGIASEIIPG
                     LHIPSFQINNAWVKEEVLDNNRDLLRGGMWGLGKIELSRNILLITHSIDTLSFPYNFK
                     NIFLEIFIQKLEECGFEIITTSNKDPLFISNYRKIFQQIEETGKNSFKIDDIPDEIII
                     RIGQELLANYIFKPFIVYQRNEKVLSLQIQYYSMAEKRWKLFENKEGKVNTIVEEEIK
                     EFIIESVVKFIEKEKITPGFYITLTEFTPYQISSISVNPIISNRKEFSIEDWKDIIIR
                     SIGLEPKNYSPKQKLLVLTRLIPLIEANVNLIEFGPKATGKTYIYRNSSIYTRIFAGG
                     KVSPAQIFYHGTYKTIGEIVRRDCIIFDELSKVILPEEMISKLKDYMVDGFFERLGLK
                     RAHSECSLVFIDNVAANNIDNLINEDIHPIIRDTAFLDRIHAFIQGWELPKILTSDEH
                     LAQGFGFTSDVLCEFFHQMKTKNFIEIIDEKIKLIGETITIRDEKAIKKISAGLLKIL
                     FPDKNINNEELLEIVEFAIELRQNVNKLLYKLLPFEFSYRNIKCQLK"
BASE COUNT         5776 a         1735 c         2544 g         4564 t
ORIGIN      
        1 cactacatat ttgcagaaac ccgtatgaaa taaggatttt tcatttttga gtggcaactt
       61 gacttgacag gtaatatttt tgtagtatag tatacaacac taatatttag tataaaaaag
      121 aggaaaaaga aaatggaaaa gaaaagaata gaagaagaga aaaagaggag atgatatgat
      181 aaaactttat gtttcgcagg aaggaaatga taattggtca ggacaaattc cgttttctaa
      241 tcaaaataat agtgatggcc catttagaac aatccaaaga gcaaaggaag aaatcactaa
      301 gcaaaaagaa aatggtaaat taagacaacc aattgttttt tatatgaaaa gaggaactta
      361 ttttcttgat gagccaatta tttttaattc agatgatagt ggtactccta aatttcctgt
      421 tctttttaca tcttttaaaa atgataaagt tgttataagt ggtggtaaaa aaatagagaa
      481 atggaatgaa accaaaataa atgggaaaga aattattgtt tttaaagtcc catttattaa
      541 agatgattta agagaaattt ttataaatgg ggaaaaaaga aacagaacaa gatttccaga
      601 aaaaaaattt ttattatata aaagagataa aattccttct gattggaaca agggggaaga
      661 tttaatttat ttaagagagg atattttagg aaacattaaa tatatagaaa atagtgaatt
      721 tattttttta cacttatggg ttgatgttca tcttcctgtt aaagaagtag attatgaaaa
      781 agggattgtg aaatttaaga aaaagagttt aaggtatctt tatgatggag atacacctgc
      841 aagattttat attgagaata tttttgatat agatcagaaa gggtcttttt attttgataa
      901 agaaaaaaaa gaaatatatt atatccctct tgaaaatgaa aatatttttg aaatagttgt
      961 tccttatctt gaaaatttaa ttatttttga aggtaattac aaaaataatg aatttgtgga
     1021 atatatcaat tttaaaaata ttattttttc acatacaaac tttacactag ataaaaattc
     1081 tgctggagac atgcaagcag gatatgagat ttcaggtagt ataaaaggga gtggttgtaa
     1141 aaatataact tttgaaaatt gtaatttttc taaacttgga aattatgcaa ttgaatttag
     1201 agatggttgt aaaaataatg agataacaaa atgtaaaatt tttgaaaatg caggaggtgg
     1261 aataaaaata ggggaatttt taagaagcga tgaagttgat ttattaaaaa atgattattt
     1321 aagacataca tatgggaata aaattgttaa ttgtgaaatt tataattgtg gtaagacatt
     1381 tcaccagtgt gttggagtat taataggaca gagttacaat aatattgttt caaattgtaa
     1441 tatctataat ctttattatt caggtatatc agttggctgg acatggggtt ataaaacatc
     1501 tcttgcttac aacaatataa ttgaatacaa ccaaattcac catatcggaa aaccagaaag
     1561 agaaaaagaa gcatttttaa gtgatatggg gggaatttac actcttggga tacaacccgg
     1621 aacaattttg agaaaaaata aaatttacaa tatttctgca tatagatatg gcgggtgggg
     1681 aatatatctt gatgaaggaa gttcagaaat tattgtttat gggaatatgg ttttttatac
     1741 gactcatggt ggatttcatc aacattatgg taagaacaat ttaataataa aaaatatttt
     1801 cgcttttggg aaagaaacac agatagtaag aagtaaagaa gaacaacatt tatcttttaa
     1861 atttgttgat aatattgttt attgggaaga aggagaactt ttaaaaggaa acttaaaaga
     1921 ttttaatttt tattttgata aaaacattta ttggaatact tctagacaaa aattcaaatt
     1981 tggtgaattt acatttgaac aatggcaaga gaaagggatg gataaaaatt caaaaattga
     2041 agaagttgat atatctttat ttttgaaaaa ataatttttt tccaacagta gtaattaagt
     2101 ttccttctat ttattattaa aatttttccc agcgaaaaat ggaaaaaatt ccttgcgcaa
     2161 tatctttttt aaatcctttt ttgttatttt taatttcaat aggaattttt tcatttgtac
     2221 aaaggtcttc aatttcagaa gaattacccc aaaatttttc tgatgcacta ataaaaactg
     2281 acttttcatt tccattattg gttaaaaaca ataatctata ttttccagaa atgccagttt
     2341 gccatttaat agtatttgtt tcctctttgc tctctactgt taattctgga agtccattac
     2401 aatctttcac aacttcaaac agtaattctt caaaatcttt gtattgacta caataatatg
     2461 gtaaaccaat aaaagtgcca atcatataaa ctttcccttt tccaaaattt tggcttatga
     2521 aaataggatt gttatttata tctcttgcca atataaaaga actattttta ctaaccagag
     2581 gagttaacca tccacttggt ttaagagaga attttttatt tttaagaaat actgcaattt
     2641 cttcctggtt aataattctt cttccctcat cactaattcc taattcccaa gtaaaatttc
     2701 tttcttctcc tggataacga taaaaaccaa gtgaagtaaa tgaatctgtt tcaccttcac
     2761 aaaaaatagt tcctcctgat tgtaaaaatt tcattaaatg aacagcaact tctggacgaa
     2821 taattaatga ccatggcata aataaaactt ttaatttata aagaatatca agatgattac
     2881 tttctacaat ttcatagggt atattacagt tttcaagaga taaaagatac cctaataaat
     2941 ttttcccagc aagttcacta tttccatcac ttgcccagtt taaatagtaa ttatctggtt
     3001 caaataaaac accaactttg ggattatcag gttgatatgt ttgaaataag tcattatatt
     3061 tattgagcaa tttccctgtt tttttcattg ctttaattct ttcttctgct tttccatcat
     3121 tacctgcaat tccaaaacca gatgattctc ttccaaatac ctcatcacgc cagcaccaga
     3181 aaattaccgc ttttgcacca cgcccatatc cattccatat ccatcgttgt tgtaaatctg
     3241 ctggaactga taggtcaacc atatgtccat ttcttgcaga accaccttga agttcactaa
     3301 cccacataac ttttcctctt gttgctgaac gaattgcttc tatcctaact cccagaattg
     3361 attcatcaaa tctgccccaa agaggaaagt gcgaggaacc aaatccatct aattggtctg
     3421 ccaaaaacca atcatttcct ctacataatg tttgttcaaa tccaccttct aaactcatta
     3481 ttgatggctg tccacagtgg gcagttatga tatgttcttt atcttctgac cttatagatt
     3541 cataacgaaa tttcatatgt ttggttgctc tccatgttaa aaactttaag aattccatca
     3601 tttcagtata agggcgagca ggaagttttc ctggaaaaac atcttcccat tgagaatatc
     3661 tacgtttcca tgccttattt aagttttcta aactttcata attttctttt aaccactctc
     3721 taaaaagatt taaggtgttt ggacaatagc aaacatatcc atctgcctgg actgcccatc
     3781 ttgtttcatt ccaacaatcc cagccaataa gatttttcac ccctgcatac cgttttgcta
     3841 tcgtttttaa aaaattttcc attaactctt gaactttaag atggtcagta caaccaccag
     3901 gagtaaggcc aacattacat tcaattctaa gactggaaat aacattattt cccatatggt
     3961 ctatcatata tgaatcagga ataatacgat gaatccagaa aggatgaatt tctgcaattg
     4021 tgctaataat tacatttagt cctttttttt caccttcaga aataagttca tcataatcat
     4081 caaatttaaa aatacccatt ttgggttcta tccatcccca gcaagcccaa agttgaatag
     4141 tattaaagcc agaatcacga attttactca aatcttcctt ccagaatttt ttttctggaa
     4201 atggtggcct ataatattga acacctaata tcataaattt ctccttttta attgtgaggt
     4261 actgctaaag ttttggttat tttcaattga aacaaaattt atatttttaa tttcatatag
     4321 atattgtaaa atatagtttt agaaagtcaa atttttgtag aaactatttt ttgattataa
     4381 tttactcaga aagaggaata taatttgtaa cgatggcaaa aaagatatga attatagggt
     4441 tattggacaa tagaaagcta acaggaagaa gggattgctg gtagatgagg aaattattct
     4501 tgagggacaa attactcaac agaaatatcc tgaaaaatta aggataataa catattatga
     4561 tgatgaacat gataaaattt atcagttttt gacaaacaat ttctaccgct ttttatcaag
     4621 agaatgaata gaagagaaaa agatatgggg aaaaaatgtt aaaaaagata ttaaccaggt
     4681 tggtatttgg cttgttttta ttatgttttg cagggaacgt attgggatta gagatgaaaa
     4741 aatatatcta tacagaggac tttgaagaaa aagatcctgt gaagttttgg acaagcaatg
     4801 gaaagtatac aataaatttt aagggattaa cagatgaaaa agcattttca ggcaagcaga
     4861 gttttaaatt agatataacc tttgaagaag ggaacttttt ttactggtgt attcctgtaa
     4921 aagttccagc agaaggggaa ttaaaatttt caggtagcat acttttagga gaagaaacaa
     4981 caggcagtgc tggattagga gtaaatgtaa ttttcccacc tacttggtat tctagttgtg
     5041 gtagttttga tacttatggg acaacaaaag gcgaatggaa actaatagaa ggtgacttag
     5101 tgaaatatgg taaagagact gctgagggag tgtttaagca acatgtttgg tgggtaagtg
     5161 ggaaagatgt aggcgtttat gttgatagag taggaatttt tctatatggt agttcaggca
     5221 agagagtagt tgtttatgta gacgatataa aaatagaagg agaagttcca acagaagaat
     5281 catataaaca agagatagag agaaggtggt cagttgtaaa aacgagattt gatgagaaga
     5341 aaatatctga ttgggaaaaa atattggtag aaagtcagca agagatatct acatttacct
     5401 ctcttactcc agaggcagag gtaataaaaa aagatgtaga agaaaagata aatattttga
     5461 agaaagaaat agaggaaata aaaaaaagag gatatatcaa taagccagag gtagatgaaa
     5521 tgaatctttc attaacacaa ctaaaagata ctattaaaac cataaagagt atttcagaaa
     5581 gagaaataaa acaaagggga tgtcttattt atattatctc cccaattagt ggacagaaga
     5641 tattacccaa agatacattt attcctggta atatatctga tgagatacaa ttagtagcaa
     5701 cgcctggaga atatgagtca gggagttttg taatacattc actttctaac ataacatctt
     5761 tgaaagtaga agttgaagat ttaaaaggag aaaaaggaat tatatcttct gaaaatatag
     5821 acataaagtt ggtaaaatgt tggtatcaat cagggactgc atggagtagt ataattcagg
     5881 aaaaaaataa cagagtatta gttccagaat tattgcttaa tgatgataat ttaataaaaa
     5941 ttgactatga aaatgaaaaa aattatctaa aactaaattt tccagaaggc gagaaatata
     6001 tttggataag cgatccagat gaacctgaag gaaatcaagc caaaatttta tctataaatg
     6061 attatccagt aaaagatagt tctgttcttt tgccagtgaa tatacccgaa aagacaaaca
     6121 aacaattctg gttaacaata aaagtccctt ctgatacaaa ggatgggata tatacaggaa
     6181 caataaaact atcagataca aaaggagaaa taggggaaat tatattaaga gtaaaagttc
     6241 tgccatttat tcttgcatct cccaaaacat attatgatat aaacgaagat tttatttcaa
     6301 gtatttatta tcgtggagtt ctacataaag attatccgga agggacaata tcatcggaat
     6361 ataaaagtaa agaacaatta aaggcagaac taaaagatat gtatgaacat gggataacaa
     6421 atcctatttg ttatcaaggt tctgatgaaa aatttttagg agagtactta aaaataaggc
     6481 aggaagtagg gatagttaac caaattcttt attctattat aagtgctaat tgtccaccat
     6541 ctgctataaa aaaaatcata gaatttgtta aagaatatgg aatagaagaa gtttattttt
     6601 atggtattga tgaggcatca ggagaaatgt tatcagccca gaggcctgca tgggaagcaa
     6661 tacacggggc aggaggtaaa gtatttgttg ctggctataa aggagataat tttgctttaa
     6721 tgggagatat acaggattta tttgttcgtg ctggttatcc atcaagagaa gaagcagaaa
     6781 agtggcatgc agttgggcat aaaatttggt gttatgccca tccgcaagca ggtccagaga
     6841 atccagaggt atggagaaga aattttggtc ttgttctatg gaaaagcaat tatgatggag
     6901 cagcgacgta tgcttatcag cattcttttg ggaatatctg gaatgatttt gaccatccta
     6961 catatagaga ccataatttt acttatccaa cagtaaacgg ggtaatagat acaattgcat
     7021 tagaaggtta tagggaagga caagatgata taagatatgg aacaacttta aaaatagaaa
     7081 tagaaaaagg gaaaaaatca aaaaatcaaa aaataaaaag tatagcactt gaggcagaaa
     7141 aatatttaga agaaatagat gtggagagag gggatttaga aactattcgt ttagagatga
     7201 ttaactacat actaaaatta acagataggt atgaaaagca aagataattt tgatagaacc
     7261 acatataaag aggaaaaaaa atgagaaaat ttgattataa taaaattaaa aatccagtaa
     7321 tgaaagataa agaagtcaat ttgagggacc cagcatgtta tttaaaagat ggtataattt
     7381 atgttttttt tacaattcat aatttaaaag atgatactgt ctgtattggg atgacaaaaa
     7441 catatgattt tattgaattt gataaaataa gaagtatttc tccaattggt tatgcttctc
     7501 ctggaaatat tataaaggta gaaaataaat ggtttctttg ttatcagcaa tataaggaat
     7561 ttccacatta cctttgttat tcctggactt acgactttga aaatttttct ccacctaaaa
     7621 aattttttaa tacagataaa acaaataaat ggaatgaaga tggaagaaca atagaccctt
     7681 atattgttga aaataatgga gtttattatt gtttttatgt tggttcaaat agatggttaa
     7741 aaggtaatgg ttataatatg atagggatgg caaaaagtta tgattttgaa aattggcaag
     7801 atgtatcaaa agaaaaacca gttattggag taaattatga ttgggaaaaa ccagatggaa
     7861 atgaaaataa ttgtgtaatt aaagtaggag aaaaatgggt tatgttatat tctgcttcat
     7921 taacatatca aaaaattgcc tatgctgaaa gttatgacct tttgaactgg aataaaagat
     7981 gtttgtgtga tgtacctatt ataaaagaaa gtgaattaag atttggcgct ccttttataa
     8041 ttgaagggct atcaaaaaat agtgaatatt atatgattta tatgggtgaa gataagaata
     8101 atagaacaag ttttattctt ctaaaaagtt atgacctgat tcactggtct taaattattt
     8161 cattcaaatt cccattagag gaaaaggggg gcttatttat gttcctgaat ttgacaataa
     8221 tgggttttca tatacaatta tttatatggg agtaaaaaaa tatcttgcaa ttgacttggg
     8281 tgcagaatca ggtagaggta ttgttgggga atttaatgga gagaaaataa aaatcaaaga
     8341 aattcataga tttcctactt ataatacaaa tgttttttct catattttct gggatgtctt
     8401 aaaaatattt gatgaaataa agattatttt aaaaaaaacg aaagaggagg gagaaataag
     8461 tggagttggg gtgacaacat ggggagtgga ttgtgcatat attggaggag gaaatctctt
     8521 actatcaaat ccatttcatt atagagatag cagaacagat ggaattatgg aggaagtttt
     8581 tagtattatt ccaaaagaag aaatttttga aaaaacagga attcaattta tgcctataaa
     8641 tactctttat caactatatt ctacaaaaaa ggaatttcca tatataatta actcaactga
     8701 aaaattatta tttatgcctg acctttttaa cttctttctt acaggaaaga aattttcaga
     8761 atatacaatt gcaacaactt ctcaaatgta taattcttta ttagaaggac aggataaagc
     8821 gccacgagga aactgggctt atgatattat tgagaaattg aatataccag ttagcattct
     8881 gccagaagta atttcgccag gaacattttt gggtaaaatg gaaaagggta tttcagaaga
     8941 aattggattg gaagatataa atatttatgc agtttgttct catgacacat cttctgctgt
     9001 tgtttctatt cctgcaaaag aagaaaaata tgcttatatt tcttctggaa catggtctct
     9061 tttaggagtt gaactatttc aaccaataat aaatgataaa agttttaatt acaattttac
     9121 aaatgaagga ggttttggtg ggagtattac atttctcaaa aacattatgg gattatggat
     9181 tttacaggaa tgcagaagat attgggaaaa agaaggtaat tactatgact atccaacttt
     9241 aactgaaatt gctataaatt cacagccatt ttattcattt attgatgtta atgatggggt
     9301 atttttattc cctggtaata tgccagagaa aataaaggaa tattgtaaaa aaacaggaca
     9361 gaaaatacca gaggaaattg gacaaattgt tcgtgttatt cttgaaagtt tggcattaga
     9421 atatagatat aacattgaaa atcttgaaca aataatagaa gaaaaaatag atgtggtaca
     9481 tattgttgga ggaggaagcc aaaataaact attatctcaa ttttctgcct catcaatgaa
     9541 aaaaattgtt ataacaggcc catcagaagc aacaggtatc ggcaatttac ttgttcaagc
     9601 aataggggat aatgaaatat cagatgtttc gcagttaaga gaaattgtaa gaaactcatt
     9661 tcctctgatt gtttttcaac caagagatac ttcaaattgg gaagaaagtt atgagaaata
     9721 taaaaaattg aaggagtttc atgatggatt gtaatttcct ttctgactct ttaattacct
     9781 atcttgaaaa aatatcatct aaaacatcaa ttcctggtgg tggaagtgtt gtatctctcg
     9841 tttcctcttt aagttgttca cttcttgata tggttttgaa ttacactatt ggaaaagaaa
     9901 aatataaaaa atatgaaaat aagttcattg atattaaaaa caaaaatcaa aagattaaaa
     9961 aggttcttta ttcttatatt gaagaagata gcaaaattta taaaaaaata aaagaaaact
    10021 caaaaaatga ggagaaacag caaatttatc ttgaaaaatc aattcaaatg cataatgaaa
    10081 tatgtaatta tatggctgat ttaattgcct tttccttttt tgttgcaaaa tatggaaaca
    10141 aacacttaat ttctgattca ggaattgttg ctattctatc tctttcatct tttaaaggtg
    10201 caaaactaaa tattttgata aatattaagt atattgatga aaatggtaag aataaatatc
    10261 tgccacttat gaagatattg aataagaaag aaaaagaact tgaaaaaaag gtagagataa
    10321 tttataacta tgcagtcgaa aaaataggag ttcaaaatgg gagatattat agatggaaat
    10381 aaaattgcac aaagaataac agataataca aaaaaagaaa tagaggaatt aaaagaaaaa
    10441 ggaatttatc caaaacttgt tgcagttagt gttggtgaaa atccggcaag ttctgtttat
    10501 ataaaacaac agaaaaaaaa ttgtgaaaaa atagggattg aatatgagtt aaaaaaatta
    10561 cctgaaagtt ataatgaaaa tcagataata tcttttattg aagaacttaa tttggacaat
    10621 aagattacag ggataatttt gcaactacct ttaccttctg gcatagatgt aaaaaatatc
    10681 caatcaaaaa tctcaccaat aaaagatgtt gaaggagtaa atccagtaaa tcttggatgg
    10741 attatttatg gcaaaccgtt tcttgcacct tgtactgcac ttgcagtaaa aaaaattatt
    10801 gattctttaa acattgacct ttatggaaaa gaagttgtta tggttggtca tagtgatatt
    10861 gttgggaaac cagttgcttt acttcttgta aatgaatttg caacaacctg tatctgtcat
    10921 attggaacat cggataaagg taaccttgag acgcatataa ggagagcaga aattttaatt
    10981 gttgcagttg ggaaggcaaa tcttatacct ggtgattggg taaaagaagg aagtattgtg
    11041 atagatattg gaataaacag agtaggagac agaattgttg gggatgttga atatgaaaag
    11101 gcaaaagaaa aggcatcctt aattactcct gttccaggag gagttggtcc agttacaact
    11161 gcaattcttt taagaaatat tgttatggca gcaaagagta ctgttaacct acgaagttaa
    11221 caagtgcttg aatatcaatg acttacgata aataagaact atttccgata aatgaaaaag
    11281 tagaaagtag caaaagaaaa ataaagaaaa agcaaaaggg gaaaaccccc tcaccccgac
    11341 ctctccccat cggggagagg agagaaagag ggaatgtatt tccttctccc ctttgcccgt
    11401 ccgccgtctt tccgccaaaa atcgcagagg attcgcaatg ttttgtggcg aattttgtgg
    11461 tggaggggag aaggctcgcc cgtagtgtat acgcccaagg gatttaaggt gaggggaaag
    11521 gtttttactg tatgtataaa ctcaaatggg tataaaactt attttcttct tataatgttt
    11581 atataggcgt tagggtatag catctacctt tgttaaaaaa ccctgttgct gtgatgaata
    11641 tatgatataa taaacttact tagtgcctaa ttatggcgga atttgggatt ttcagaaaat
    11701 acaaaataaa atcagcaggt ctttatggaa gttgtgcaaa aagagaacaa atattaaatt
    11761 ctgatgtgat atatgagtaa gaactgaaaa catatcggaa aaaataaaaa atgattattt
    11821 taattattgg gttaattatt gggttaatta ttatagcagt ggaaatttat tttgttgtaa
    11881 aagcaaaaag aaaagaaaat atggcggaag agaaaaagaa aaaaattgaa gaaactgctg
    11941 aaataataaa aaataaagca atatcagaag cagaagaaaa attaaaagga gcgaatgaaa
    12001 aacttgaaaa tataaaaagg gaaatggcgg aaatacaaca ggaaaaaaat agatccaaaa
    12061 aagaaagaga aacaattgaa aaaataaagg aggaattaaa agaagagaaa aagagtttaa
    12121 ataaaaaaga gtttattctg atacaaaaag aatatgaatt agagaaaaaa gaacaagatt
    12181 tgagctttca aaagaaaatg aatgatgaag aatttatacg acaagaagaa aatcttaata
    12241 aactaagagc tcgtataaaa gaagaatctt taaaggtaga tgagacaaaa gcagagattc
    12301 gcgagaaaat aatgctatta caagaacaag agaaaagttt agcgatgaaa gataaagaga
    12361 gtaaaaaaat agaagaaaat ttgagggaac aacagagaga attgataaaa agagaaaaat
    12421 gggtaatttc taaagaaaaa gaattaaatg aaaaaatagg agaatacgaa aaaaagttag
    12481 aagaaatagc agaaatttca aaaaaagatg ctattgacat acttttgaaa aaaatggaaa
    12541 atgaagccca aaaagagttt ttggaaaaag ggaaattaag aagttgggat gattttttaa
    12601 aataaaaggg attaaaaaaa atgcaattag acgaaaaatt aaaaaaccta tttccaaatt
    12661 tagtagttaa caaaaaactt gctttgacaa aagaagtaag tagattacca agatttatta
    12721 gcgaatattt gataacgaaa tttttggaaa atgacaaatt aaatacagaa aaactttata
    12781 attttttaaa agagcattat atatctcctg aagaaagaaa cgatgcttta catcaattaa
    12841 gaaaagaagg gataaaaata attatggatg aattttatgt agaaatgaaa gtttcaggag
    12901 gtattgcttc agaaattatt cccggtttac acattccctc ttttcaaata aataatgcgt
    12961 gggttaaaga agaagttctt gataataaca gagatttatt aagaggtgga atgtggggac
    13021 ttgggaaaat agaattatcc agaaacattt tgctaataac acattcaatt gatactctgt
    13081 cttttccata taattttaaa aatatatttt tagaaatttt tattcaaaaa cttgaagaat
    13141 gtggatttga aataataaca acaagtaata aagacccact ttttatttca aattatagaa
    13201 aaatttttca acaaatagaa gaaacaggaa aaaattcttt taaaattgat gatataccag
    13261 atgaaataat aataagaatt ggacaagaat tgttagcaaa ttatatattt aaaccattta
    13321 tagtatatca acgaaatgag aaagttttat ctttacaaat tcaatattat tcaatggcag
    13381 aaaaaagatg gaaacttttt gagaataaag aagggaaagt aaatactatt gtagaagaag
    13441 aaataaaaga atttataatt gagtcagtag tcaagtttat tgaaaaagaa aaaataactc
    13501 cgggattcta tattactttg acagaattta ctccttatca aatttcttct atatctgtca
    13561 atccaataat ttcaaataga aaagaatttt caattgaaga ttggaaagac ataattatcc
    13621 ggtcaattgg tttagagcca aaaaactatt cacctaaaca aaaactattg gttttaacaa
    13681 gattaattcc acttattgaa gccaatgtta atttaataga atttgggcca aaagcaacag
    13741 gaaaaacata tatttatagg aactcttcaa tatatacgag aatttttgca ggtggaaaag
    13801 ttagtccagc ccagatattt tatcacggga catataaaac tattggggaa attgtaagaa
    13861 gagattgtat tatttttgat gaactttcaa aagtcatttt accagaagaa atgataagca
    13921 aattaaagga ttatatggtt gatggatttt ttgaaaggtt gggattaaaa agagcacata
    13981 gtgaatgcag tttagtattt atagataatg tagcagcgaa taacattgac aatttaatta
    14041 atgaagatat ccatcctata atcagggaca ctgctttttt agatagaatt catgctttta
    14101 ttcaaggatg ggaattacct aaaattttga cctctgatga acatcttgcc caaggatttg
    14161 gatttacttc tgatgtatta tgtgaatttt ttcatcaaat gaaaactaaa aatttcatag
    14221 aaattattga tgaaaaaatt aaactcattg gggaaactat aaccattaga gatgaaaaag
    14281 caattaaaaa aataagtgca ggactactta aaattttatt ccctgataag aatatcaata
    14341 atgaagaatt attagaaata gtagaatttg ctattgaatt aagacaaaat gtaaataaac
    14401 ttctttataa attattacca tttgaatttt cctatagaaa tattaaatgt caactcaaat
    14461 aattttattt gaaccgctct taatggcata tgttaaccta cgaagttaac aagtgcctga
    14521 atatcaatga cttacgataa ataagaacta tttccgataa atgaaaaagt agaaagtagc
    14581 aaaagaaaaa taaagaaaaa gcaaaagggg aaaaccccc
//
LOCUS       DAOOVW010000041        13716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_2988_length_13716_cov_9.740283, whole genome shotgun sequence.
ACCESSION   DAOOVW010000041 DAOOVW010000000
VERSION     DAOOVW010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13716
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2988_length_13716_cov_9.740283"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(626..1495)
                     /locus_tag="PLW95_06200"
     CDS             complement(626..1495)
                     /locus_tag="PLW95_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HOV22255.1"
                     /translation="MEDKEIKKKILKAQKNELTEHFIYKKLASLIKDEKNKKILEDIG
                     EEELKHYEVFKKITKTDVKQNKLKFYFYVFLSRMFGLTFSLKLMETGEEFAQKEYEKI
                     TKVISGVEWILKDENKHEKEIISLLDEEKLKYVSSIVLGLNDALVELTGALAGFTFAL
                     QKTKLIVIVGFITGIAAAMSMAASGYLSAKQEEGEKNPLKSSMYTGIAYIITVLFLLL
                     PYLVFKNVFLCLGFVMVNALIVILVFTFYVSVAKEVSFKKRFFEMASLSLSIAVINFL
                     IGILIRKGFGIDV"
     gene            1665..3110
                     /locus_tag="PLW95_06205"
     CDS             1665..3110
                     /locus_tag="PLW95_06205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOV22256.1"
                     /translation="MAHQRKRKIEKGLVDGELSHSIWKLAGPMMIAGALHDLFSMVDL
                     FFVGKLGHIEVAALAIAGTTMSVLMMLVQGIAVGTMVLIAHFTGEKNYEMSDNVLGQT
                     FIWSLIGASIMLPVSFFLVEPILKLFGATGNILVYAADYLRINFNYSLVIFLFVGISN
                     ALQGSGDARTPLYALVIGNVLNIILDPLYIMGYGPFPALGVAGSAVATVLTRGVGIIY
                     LFIHLLFGHSTIHFKIKYLKPIPFLMKRIVSIGFFASLQALIREISFLFLMRLVTSFG
                     AVTLAAYGIGSRLRSFIMVPGFGFASAAAVLVGQNLGAKKPKRAEKAAWRALRYYEFL
                     LTPIVIIFLFFAPTLVGFFNNNKEVITIGSSFLRYLAITFPFLAFSLVFSQAMNGAGD
                     TRTPTIINAIGQLIFRIPFAYFCALVLGLGYKGIWLGINASDIVQGIGMTMVFRSGHW
                     KKAFARHKNKLLKDDRQMATSLLDTETSSLN"
     gene            complement(3180..3251)
                     /locus_tag="PLW95_06210"
     tRNA            complement(3180..3251)
                     /locus_tag="PLW95_06210"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3217..3219),aa:Asn,seq:gtt)
     gene            complement(3446..3736)
                     /locus_tag="PLW95_06215"
     CDS             complement(3446..3736)
                     /locus_tag="PLW95_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010692565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOV22257.1"
                     /translation="MPTYEYECEKCGYRFEKFQKMTEPPVEVCPECKEKKVKRLIGLG
                     GGVIYKGAGFYTTEYRSEEYKRKANEEKQVVTSSSSSSCAGCSSSNCSTCKK"
     gene            complement(3817..4584)
                     /locus_tag="PLW95_06220"
     CDS             complement(3817..4584)
                     /locus_tag="PLW95_06220"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOV22258.1"
                     /translation="MEKQITFESEGKKLFGVLHLSNKKKSPGIVLFHGFSGQKAESHF
                     IFTHLARFLCKEGISVLRFDFMGSGDSEGEFVDKTLLTEMKDGENAVNYLKNLKEIDR
                     NRIGVLGLSLGSVTAVYTASKLNIKSLCLWSGLAYPSVISRQLTRKLKKQLEEKGEGY
                     LTGTGLSISKDFVESLKHIKPLEFAKQFKGNVLIIHCKDDTVLSLTHPFAYFENFHYN
                     AGFSELVIFEKGRHTFVIEKTEKDVLETTLKFFKETL"
     gene            complement(4597..5502)
                     /locus_tag="PLW95_06225"
     CDS             complement(4597..5502)
                     /locus_tag="PLW95_06225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOV22259.1"
                     /translation="MRNVLIIREGSFGDLILTLPVVQSLKKNNFCVYLAGKGIYKNFF
                     EIYSDINGFYSFDNTFFLPLFSGERNQLLSEFLNNFDLIIFYEDEKTLQGKTLRKIFK
                     GEILFHPVKNEHLKMHIIDYLLLPVRNFPTEIEKYPFLKIENKEENIIVIHPGSGCSH
                     KNYKKENFFEIIKKIKDKEFKVILGPAEFDQFQWWNNKIGQEKILKTSFLQDIVDIVK
                     TTKIYIGNDSGITHLFAASGVKTIAIFGPTSPFIWGPRGKKVKIIFREVGCNPCSKEK
                     MKNCKEKLCFEQIEIKDIINFIENF"
     gene            5667..5738
                     /locus_tag="PLW95_06230"
     tRNA            5667..5738
                     /locus_tag="PLW95_06230"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5699..5701,aa:Thr,seq:cgt)
     gene            complement(5751..6962)
                     /locus_tag="PLW95_06235"
     CDS             complement(5751..6962)
                     /locus_tag="PLW95_06235"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HOV22260.1"
                     /translation="MVEISPLKGYTYNLEKVGNISNVISPPWDVIDSEYEKKLKLLSP
                     YNVINLISKNENPQEVFQKFNKWIKEKIIIEDESDCFYFMKHTFLVDNKQMERNGVFA
                     LLKLETFEKGNIIPHERIFEKYKVNRYKLIEKCRANFSPVFMLYQDNNFIFENEIKKL
                     NGEIKGTINNEIFSFGKIKDKNKTEIIKNIFKDKKLIIADGHHRYNAALHFYLDNPTP
                     SAGKVLVFLVNIKSPGLLILPTHRYVIEDIPFFKKKQLIEKFFEIQKVPSLFEMKEKM
                     EQFQDSHCFGVYDNGEFFVLILKSKDVIGKIAEKHSENWSELDTVILHEFIFPEIFEI
                     KNNEFFFHISPEYLIKEREKRRKGIIFFLNPIKKEQFLKICFCGEVMPQKSTYFYPKV
                     PSGLVIYKFNS"
     gene            complement(6999..7727)
                     /locus_tag="PLW95_06240"
     CDS             complement(6999..7727)
                     /locus_tag="PLW95_06240"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOV22261.1"
                     /translation="MLTKFFRKKRNMKIVLWIVALMIIPGFLIWGVNISGAQRRSSCA
                     AIVNRDEITLREFYTRLSDVEESYRQIFGDKYDEIKNNLNIEEGVLEDMIREKLLLQQ
                     ARKKRIRVFNNEIIEAVKSDPVFKNDAGKFDEKKYREIISNIPDEELKKIEDDVREKI
                     TFEKLKNLVVSEGNIKVSDEEVDDYIKRNNLKDVDKEQLRKVLLWQKMEKYFNDWYSQ
                     IRKTAKVEIYLPLKKKSENQKIDE"
     gene            complement(7730..8392)
                     /gene="rsmI"
                     /locus_tag="PLW95_06245"
     CDS             complement(7730..8392)
                     /gene="rsmI"
                     /locus_tag="PLW95_06245"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOV22262.1"
                     /translation="MLYIVSTPIGNLDDITFRAIETLKNVDLILCEDTRETLKLLNYY
                     GIKNHLLSYYKDNEYKRIGKVIEMLKEGKEIALVCDRGTPGISDASFLLVREAYKNNI
                     KVIPVPGPSALTSAISVSALPSSQFTFYGFLPKGENKKRKLMESLIEREETLIFFESV
                     HRINETLKIIKDVFQNREMTVCRELTKKFEEIKYGSVEEVVDYFLSKKNKGEFVILIK
                     GR"
     gene            8486..9700
                     /locus_tag="PLW95_06250"
     CDS             8486..9700
                     /locus_tag="PLW95_06250"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOV22263.1"
                     /translation="MEYEEILPEIIGKNENKILLLVLDGLGGIPHPETGKTELETANI
                     PNIDEFAKNGSCGLSIPVLPGITPGSGPGHISLFGYDPIKVLIGRGVLECLGIGVNLG
                     KEDIAIRGNFATIDENDIVTDRRAGRISTDENKKIVDLISKNIKEINGVKISINTVKE
                     HRCAIVLRGKNLSPDVSENDPQKENLPLKEILPLNQEGKFTASVLNEFEKKVKEILKN
                     VNSKAKVILLRGISKLPDVPTFQEKYKLKSACIATYPMYKGISKVVGMDIIEVGETVE
                     EEFDKLEKVYDNYEFFFLHIKKTDSMGEDGNFNGKVKFLEDVDKKIWRLKKLNFSAMA
                     ITGDHSTPSVLKGHSWHPVPLVIVSPNMIRDDIERFSERECAKGILGTIYAKQIMYLL
                     LASSLKLGKFGA"
     gene            9702..10997
                     /gene="rsxC"
                     /locus_tag="PLW95_06255"
     CDS             9702..10997
                     /gene="rsxC"
                     /locus_tag="PLW95_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOV22264.1"
                     /translation="MFRGGVEFIDFKSLSKEEPIENFKEPDKVVIPLSQHTGIPAKLV
                     VRKGDNVLKGQVIGEIAGFISSSVHSSISGKVIGVEDILTHTYKISPAVIIENDGSGK
                     EIEKKKNDWFSLSPSEIIELVSKNGVVGLGGATFPSHVKLVIPHGKKCEAVILNGCEC
                     EPFLTCDYRVLKENTEDVIIGLEIICKTLGVKKAYIGIEQNKIDIAEKIKKVIKNKSC
                     EIELKVLPKKYPQGSEKHLIKSITGKEVPSGGLPIDVGIVVFNVQTAISIKNAVCDGE
                     PLIERVITVSGLVERPKNLRVKIGTSISEVITYCEGELEEGSKIVIGGPMMGMNIPSS
                     DIPVIKGTTGILILPEEILPEEIEPCIRCGKCIDACPMNLCPAEIGRYAELKKWEEVE
                     KLSVSDCIECGCCSYICPSKRPLVELFKWAKTKIKRKKE"
     gene            10994..11911
                     /locus_tag="PLW95_06260"
     CDS             10994..11911
                     /locus_tag="PLW95_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOV22265.1"
                     /translation="MKKISWAPHIHSNETTGKIMLSVIIALIPPLIGAIWFFGLYSLK
                     IIVVSIVSCVLSELLSQIIFKKKIRIFDGSAIITGILLAFVLPPRIPLWVVGFGGFLA
                     IFLIKEIFGGIGFNIFNPALASRAILLACFPVFMTHWIQPFDYSIDAITTATPLTILK
                     ENFLQQLPSLWQMFIGIRAGCLGETSVLLLLLGAIFLLCRKVITLHIPVSYILTVAIL
                     SVIFKQNVAMQVMAGGLILGAFFMATDYVTSPITKKGKIIFGIGCGIITFLIRLKGSY
                     PEGVCYSILFMNMLVPLIDKYTLPKKFGR"
     gene            11914..12462
                     /locus_tag="PLW95_06265"
     CDS             11914..12462
                     /locus_tag="PLW95_06265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HOV22266.1"
                     /translation="MNKKEIVGGILILTIITSLSGYLLAYVYKVTKPAIEKQQKIEED
                     NINKEIFPDGVEFHEVEQGDIKYISVLGSEGKEIGKIFTTTSPGYGGDIKIKIGVDED
                     LKIKGVKILQHNETPGLGAKITEKKFLSQFDGKGKEKIYLKKDNSEGEIDTITGATIS
                     SKAVTEGVRKLLETIEVEKNEN"
     gene            12452..13054
                     /locus_tag="PLW95_06270"
     CDS             12452..13054
                     /locus_tag="PLW95_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOV22267.1"
                     /translation="MKIDFKNFVNALWKENAIFYLMLGLCPFLAVSSSVRDSIGMGIA
                     VIFVLAGSNVTISLIRKFVPSHVRIPAFIVVIATFVTIVDYSLAALSPQIHRNLGVYL
                     PLVVVNCIILGRAEAYASKNNVISSLLDGLGVGIGFALVIFVVSIVRELFGNGTILGH
                     QIFGANFTPFLIMIMPPGAFIVIGFFIALKQGIERRGKKC"
     gene            13048..13638
                     /locus_tag="PLW95_06275"
     CDS             13048..13638
                     /locus_tag="PLW95_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit A"
                     /protein_id="HOV22268.1"
                     /translation="MLNQANLGLIFLSAFLVNNILLIKFLGLCSFFGLSKDIKSSIGM
                     GLAVIFATVMACCLSWAVYNLLLLPLHLEYLRTATFILTIASFVQFEEIIIRKKLPVL
                     YKAFGIYLPLITTNCAILACAFLGVDYKLSFIQNLVFSIALPAGYALAIILFASIREK
                     IDVAPLPEVFKGAPISFITAGLMSLAFLGFKGLFKL"
BASE COUNT         4581 a         2052 c         2057 g         5026 t
ORIGIN      
        1 ttcatttata aagagaaaaa gatggcttct tccttattca gttaaaaaat aatttgacaa
       61 gtcaagttcg ccatttaaca atttctgaca tttaacccct aacttctcat aaatcaagta
      121 accctaaaat cccgttttct tataactatg gagaatagaa aattatcaaa aacaaaaagt
      181 agtgtagatt tagaattgtg aatttgaata atagatgtat ttatattata actatgaaaa
      241 aatttaaaga attcacattt tgaaatgggg taatactatt tttacattaa aggtgtaata
      301 aaaatcagag ccctcacccc ttcccctctg ggcgtagtcc acaggctcgc ccgttgggct
      361 atgcccagag ggagccgtag caaaaagcga ggatccaacg ggcgagcctc tcccctccgc
      421 cacattcgcc tatcattgcg aatccgctgc gatttttggc ggaaagattg gcggacagac
      481 taaggggaga agggaaaaaa ctttctgacg ctaatacatt ataaggattt tatgatataa
      541 tacactcgct taatgcctaa ttttggcgga atttgagata aagattagag tttttgtttc
      601 ttggaaaatt ttttatagat attttttata catcaatacc aaagcctttt cttattaaaa
      661 ttcctataag aaaatttata actgcaatac tcaaacttaa agatgccatc tcaaaaaatc
      721 tctttttaaa agaaacttct tttgcaactg aaacatagaa agtgaaaaca agtataacaa
      781 taagagcatt taccataaca aaacctaaac ataaaaaaac atttttaaaa acaagatatg
      841 gtagaagtaa aaaaaggaca gttataatat atgcaattcc tgtgtacatt gacgatttta
      901 aaggattttt ctccccttct tcttgttttg ctgataaata gccagaagca gccattgaca
      961 tagcagcagc aattcctgtt ataaatccaa caataactat caattttgtt ttctgtaagg
     1021 caaatgtaaa accagcaaga gcccctgtta actcaacaag ggcatcattt aggcccagaa
     1081 caattgaact tacatatttt aatttttcct catctaaaag ggatataatt tctttctcgt
     1141 gtttgttttc atctttaagt atccattcta ctcctgatat tacttttgtt attttttcat
     1201 attctttttg tgcaaattct tcccctgtct ccatcaattt taaggaaaat gtaagtccaa
     1261 acattcttga tagaaataca taaaaataaa atttaagttt attttgtttt acatcagttt
     1321 tagttatttt cttaaagacc tcatagtgtt ttagttcttc ttccccaata tcttcaagaa
     1381 tttttttatt cttttcatct tttataagag atgcaagttt tttataaata aaatgctctg
     1441 ttaattcatt tttctgtgct ttcaaaattt tcttttttat ttctttatct tccattttta
     1501 tttcctttct tcaattatac aataaaacag aaaaaaatta aggcagaata ataaaagttg
     1561 acaggaaaga aatatggaag tataacaggt aaatagataa aaaaatccca ccattatttg
     1621 attgttttta aatttatatt gatatactga ataggaatta taaaatggca catcaacgaa
     1681 aaagaaaaat tgaaaagggt ttagtagatg gtgaactttc ccattcaata tggaaattag
     1741 caggtcccat gatgatagca ggagcattgc atgacctttt ttctatggtt gacctgtttt
     1801 ttgttggaaa gttaggacat attgaggtgg ctgcacttgc aattgcaggg acaactatgt
     1861 ctgttctgat gatgctggtt caaggcattg cagttggaac aatggtactt atagctcatt
     1921 ttacaggaga aaaaaactat gagatgagtg ataatgtgtt aggacagaca tttatctgga
     1981 gtcttattgg tgcatctata atgttgcctg tttctttttt tcttgtagag ccaatcctaa
     2041 aactttttgg tgcaacagga aatattctgg tttatgctgc tgactactta cgaataaatt
     2101 ttaattattc tcttgttatt tttctttttg ttggaataag taatgcttta cagggttcag
     2161 gagatgcgag aacaccttta tacgcacttg taataggtaa tgttcttaat ataattttag
     2221 accctttata tattatgggt tatggtccct ttcctgcatt gggagttgct ggttctgctg
     2281 ttgcaacagt tttaacaaga ggagttggta ttatatatct ttttatccat cttctttttg
     2341 ggcattctac aattcatttt aaaataaaat atcttaaacc aattccgttt cttatgaaaa
     2401 gaattgtaag tatcggattt tttgcttcac ttcaggcgtt aatccgtgaa atttcttttc
     2461 tctttttaat gcgtcttgtc acatcctttg gtgctgttac tcttgctgct tatggaattg
     2521 gttctcgact gaggtctttc ataatggtac ctggttttgg atttgcaagt gctgctgctg
     2581 ttcttgtggg acaaaattta ggagcaaaaa aaccgaagag agcagaaaaa gcggcatggc
     2641 gtgcgcttag atactatgaa tttctactta caccaatagt tataattttc ttgttttttg
     2701 ctcccacatt ggtaggattt tttaataata acaaagaagt aattactatt ggcagttctt
     2761 ttttaaggta tttagcaatt actttccctt ttcttgcttt ttcacttgtt ttttcacagg
     2821 caatgaatgg tgctggtgat acaagaacac caactattat aaatgcaatt ggtcaactta
     2881 tttttagaat accttttgca tatttttgtg cattggttct cggattgggt tataaaggaa
     2941 tatggttagg tataaatgct tctgatattg tgcaaggtat tggaatgacg atggtttttc
     3001 gctctggaca ctggaaaaaa gcatttgcga gacacaaaaa caaattactg aaagatgata
     3061 gacaaatggc aacttcccta ttagatactg aaacctcttc tcttaactaa aacaaaattc
     3121 tttaacacta tttccttatt ttatagaagt ataatatctg ctaataagaa aacaaatagc
     3181 tccggtggta ggattcgaac ctacaacctt gcggttaaca gccgcttgct ctaccattga
     3241 gctacaccgg aattttcaat ttttaacttt ttaactgtca ataaaatatt gtttttcaac
     3301 cttgctctac tccgccataa atttcgccac acaacatggc gaatccgacg cgctttctgg
     3361 cggacatggc ggacttcact tcgtttcgcg ttgagctaca ccggaattcg taaataataa
     3421 tatcagaaaa cattagattt gtaaattatt ttttacaggt tgaacaattt gaagaagaac
     3481 aacctgcaca tgatgaagaa cttgaagaag ttactacctg tttttcttca tttgcctttc
     3541 ttttatattc ttcacttcta tattctgttg tataaaaacc agcaccttta tatataactc
     3601 caccaccaag tcctatcagc cgtttaactt ttttttcctt acattcaggg catacttcta
     3661 caggtggttc tgtcattttt tggaattttt caaatctata tccacacttt tcgcattcat
     3721 attcataagt aggcattttt ctcctcctgt ttttctttaa ctaattaaaa taccgaaaaa
     3781 tttcaaaaat gtaaatatct aaatttatga atatttttat agtgtttctt tgaaaaattt
     3841 taatgttgtt tctaaaacat ctttttctgt tttttcaata acaaaagtat gtcgtccttt
     3901 ttcaaaaatc acaagttctg aaaaccctgc attataatga aaattttcaa agtatgcgaa
     3961 aggatgtgtt aaggataaaa ctgtatcatc tttacaatga ataattaaaa catttccttt
     4021 aaattgtttt gcaaactcca atggttttat atgctttaaa ctttctacaa agtccttgct
     4081 tatagaaagt cctgtccctg tgagataacc ttcccctttt tcttctaatt gtttttttag
     4141 ttttcttgtt agttgtcttg atataactga tggataagca agtcctgacc ataaacaaag
     4201 tgattttata ttcaatttcg atgcagtata aactgctgta acacttccta atgaaagtcc
     4261 aagaactcca attctattcc tgtcaatttc ttttaagttt tttaagtaat ttactgcatt
     4321 ttccccatct ttcatttctg ttaaaagtgt cttatcaaca aattctcctt cactatctcc
     4381 tgaacccata aaatcaaatc ttaaaacaga tattccttct ttacagagaa atcttgcaag
     4441 atgagtaaaa ataaaatgac tctctgcttt ttgtcctgaa aaaccatgaa ataaaacaat
     4501 tcccggggat ttctttttat tacttaaatg caagacccca aataattttt tcccttcgct
     4561 ttcaaatgtt atttgttttt ccaattttta tcctttttaa aaattttcta taaaatttat
     4621 tatatcttta atttctattt gctcaaagca caatttttct ttgcaatttt tcattttttc
     4681 tttgctacat ggattacagc caacttctct gaaaattatt tttacttttt tccctctcgg
     4741 tccccagata aaaggagaag ttgggccaaa tatagcaatt gttttaactc ctgatgcagc
     4801 aaaaagatgt gtaatacctg aatcgtttcc tatataaatt tttgttgtct tcacaatatc
     4861 aacaatatcc tgaagaaaag atgtttttaa aattttttcc tgtcctattt tattgttcca
     4921 ccattgaaat tggtcaaatt cagcaggacc aagaataact ttaaattctt tatcctttat
     4981 cttttttata atctcaaaaa aattttcttt tttgtaattt ttgtgagaac aaccactacc
     5041 aggatgaatt actattatat tttcttcttt attttctatt ttcaaaaaag gatatttttc
     5101 aatttctgtg ggaaaatttc taacaggtaa aagaagatag tcaattatat gcatttttaa
     5161 atgttcattt tttactggat gaaataaaat ttcaccttta aaaatctttc tgagtgtttt
     5221 tccttgaagt gttttttcat cttcataaaa aattattaaa tcaaaattgt tcaaaaattc
     5281 tgataataat tgatttctct caccagaaaa aagaggaaga aaaaaggtgt tatcaaaaga
     5341 gtaaaatcca ttaatatctg aataaatttc aaaaaaattt ttatatattc ctttacctgc
     5401 taaataaaca caaaaattat tttttttaag tgattggaca acaggtaatg taagaattaa
     5461 atctccaaaa gaaccttccc ttattattaa aacatttctc atttttaatt tcaaataata
     5521 ttgacaattt ttatctgtaa ggtataatat ttctgctttc tctctgcttg tgaatgcagg
     5581 taagtagcat ggctttcggg cgccggcgcg attcggcgcc cttttttttt atcctcaatg
     5641 acaaaaactt gatataataa aaataagccc gtgtagttca gtggtagaac ggctcattcg
     5701 taatgagcaa gtcaggggtt caagtcccct cgcgggctta tgtcttattt ttaagaattg
     5761 aatttgtaaa taacaagacc tgatggaact tttggataga aataagttga tttttgaggc
     5821 ataacttctc cacaaaaaca tattttaaga aactgctctt ttttaattgg gtttaaaaaa
     5881 aatataatac cttttctcct tttttctctc tcctttatta aatattctgg cgaaatatga
     5941 aagaaaaatt cattattttt tatttcaaaa atttcaggaa atataaactc atgaagtata
     6001 actgtatcta attcagacca gttttcacta tgtttttctg caatttttcc aattacatct
     6061 ttacttttta atattaaaac aaaaaattct ccattatcat aaacaccaaa acaatgacta
     6121 tcctgaaatt gctccatttt ctctttcatc tcaaaaagtg aaggaacttt ttgaatttca
     6181 aaaaattttt caattagttg ttttttcttg aaaaaaggaa tatcttcaat tacatatcta
     6241 tgagttggta aaattaaaag gccaggggat tttatattaa caagaaatac aagaacctta
     6301 cctgctgatg gggtagggtt atcaagataa aaatgaagcg ctgcattata tctgtgatgt
     6361 ccatcggcaa taataagttt tttatcttta aagatgtttt taattatttc agttttattt
     6421 ttatctttta ttttaccaaa agaaaaaatt tcattattta tagtaccttt aatttcacca
     6481 tttaatttct ttatttcatt ttcaaaaata aaattattat cctgatataa cataaaaaca
     6541 gggctgaaat ttgctctaca tttttctata agtttatatc tattaacttt atacttttca
     6601 aaaattcttt catggggaat aatattgcct ttttcaaatg tttcaagttt aagtaaagca
     6661 aaaaccccat ttctttccat ctgtttattg tccactaaaa aagtgtgttt cataaagtaa
     6721 aaacaatcac tttcatcttc aataattatt ttttctttta tccatttatt aaatttttga
     6781 aaaacttcct ggggattttc attttttgat attaaattta taacattgta aggagataat
     6841 agtttaagtt ttttttcata ttcactatca ataacatccc atggaggaga tataacatta
     6901 cttatattcc caactttctc taaattatat gtataacctt ttaaaggact tatttctacc
     6961 attttttctc catatctttc taattctgtc tcctgtcttt attcatctat tttttgattt
     7021 tcacttttct tttttaatgg taaatatatt tcaacttttg ctgtttttct tatctgagaa
     7081 taccaatcgt tgaaatactt ttccattttc tgccataata aaacctttct aagttgctct
     7141 ttatcaacat cttttaaatt gttccttttt atataatcat ctacctcttc atcactaact
     7201 tttatatttc cttcagatac aacaagattt tttaatttct caaaagttat tttttccctg
     7261 acatcatctt caattttctt taactcttca tccgggatat tgcttataat ctctctatat
     7321 tttttttcat caaatttccc agcgtcattt ttaaaaacag ggtctgactt aactgcttct
     7381 attatttcat tattaaagac ccttattctc ttttttcttg cctgctgtaa taaaagtttt
     7441 tctctaatca tgtcttcaag aacaccttct tctatattca ggttgttttt tatttcgtcg
     7501 tatttatctc caaaaatctg tctgtaactt tcttctacat cagaaagacg agtataaaat
     7561 tctctcaaag taatttcatc tctattaaca attgctgcac aactacttct tctttgagcc
     7621 cctgaaatat taactcccca tatcaaaaaa cccggtatta tcataagtgc tacaatccag
     7681 aggactattt tcatattcct ttttttcctg aaaaattttg ttagcatttt tatctccctt
     7741 ttatcagtat aacaaattct cctttatttt ttttacttaa aaaataatca acaacttctt
     7801 caacacttcc gtacttaatt tcttcaaact tttttgtaag ttctctacaa acagtcattt
     7861 ctctattttg aaaaacatct tttataattt ttaatgtttc atttattcta tgaactgact
     7921 caaaaaaaat caatgtttct tctctttcaa tcagcgactc cattaacttc ctctttttat
     7981 tttccccttt tggtaaaaat ccataaaagg taaattgaga tgaaggaaga gcagaaacag
     8041 aaattgcaga agttaaggcg gaaggtccag gaacaggaat aacttttata ttatttttat
     8101 atgcctcccg aacaagtaaa aaagaagcat ctgaaattcc aggagtccct ctatcacaaa
     8161 caagagcaat ttcttttcct tcttttagca tttcaattac tttcccaatt cttttatatt
     8221 cattatcttt ataataagat agaagatgat tttttatacc ataataattt aataatttca
     8281 atgtttctct tgtatcttca cataaaatta aatcaacatt ttttaatgtt tcaattgctc
     8341 tgaaagttat gtcatcaaga tttccaattg gagttgaaac gatatataac attataaaaa
     8401 tactcctttt ttaatttaaa attttacaat ttattttaat aagagtaaaa taatttaaat
     8461 tttttaattt ataggaggaa aaaaaatgga atatgaggaa attttaccag agattatagg
     8521 gaaaaatgaa aataaaatac ttcttcttgt tttagatgga cttggaggta ttccacaccc
     8581 ggaaacagga aaaacagaat tagaaacagc aaatattcca aatattgatg aatttgccaa
     8641 aaatggttcc tgtggtcttt caataccggt tcttccggga ataactcctg gcagtgggcc
     8701 cggacatatt tctctttttg ggtatgaccc tattaaagtt ctcataggaa gaggtgttct
     8761 tgaatgcctt ggaataggtg taaatcttgg gaaagaagat attgcaataa gaggtaattt
     8821 tgcaacaatt gatgaaaatg acattgtgac ggatagaaga gcaggtagga tttctactga
     8881 tgaaaataaa aaaattgttg atttaatttc aaaaaatatt aaagaaataa atggggtaaa
     8941 aatttcaata aacactgtaa aagaacatag atgtgcaatt gttttaagag ggaaaaacct
     9001 ttctcctgat gtaagtgaaa atgacccaca aaaagaaaat ttaccgctta aagaaatatt
     9061 acctttaaac caggaaggta aatttacagc tagtgtttta aatgagtttg aaaaaaaagt
     9121 aaaagagata ttgaaaaatg ttaattcaaa agcaaaagta attctactta gaggtatttc
     9181 aaaacttcct gatgttccaa cttttcaaga aaaatacaaa cttaaaagtg cctgtattgc
     9241 tacttatcct atgtataaag gtatttcaaa agttgttggg atggatataa ttgaagttgg
     9301 tgaaacagtt gaagaagaat ttgacaaact tgaaaaagta tatgataatt acgaattctt
     9361 ttttttacat ataaaaaaga cagatagtat gggcgaagat ggaaatttta atggtaaagt
     9421 aaaatttctt gaagatgttg ataagaaaat atggcgtctt aaaaaattaa acttttcagc
     9481 aatggcaatt acaggtgacc attcaacacc ttctgtttta aaaggtcatt catggcatcc
     9541 agttccactt gtaattgttt cgccaaatat gatacgagat gatattgaaa gattttcaga
     9601 aagagaatgt gcaaaaggta tattaggaac aatttacgca aagcaaatta tgtatttgct
     9661 acttgcaagt tcattaaaat taggaaaatt cggagcataa aatgtttaga ggcggagttg
     9721 aatttattga ttttaaatct ctcagtaaag aagaaccaat tgaaaatttc aaagaaccag
     9781 ataaagttgt tatccctctt tcacagcata caggaatacc tgcaaaattg gttgtgagga
     9841 aaggagataa tgttttaaaa gggcaggtta ttggagaaat tgctggtttt ataagttctt
     9901 ctgttcattc ttcaatatca gggaaagtta ttggggttga agatatttta actcatactt
     9961 ataaaatatc cccagcagta ataatagaaa atgatggaag tggaaaagaa atagaaaaaa
    10021 agaaaaatga ctggttttct ttaagtcctt ctgaaataat tgaacttgtt tcaaaaaatg
    10081 gagttgtagg acttggtgga gcaacttttc cttctcacgt aaaattagtc atacctcatg
    10141 gcaaaaaatg tgaagcagtt atattaaatg gatgtgaatg cgaaccattt ttaacctgtg
    10201 attacagggt tttaaaagaa aatacagaag atgtaattat tggcttggaa attatatgta
    10261 aaacattagg agttaaaaag gcatatattg gaattgaaca aaataaaatt gatatagcag
    10321 aaaaaattaa aaaagtaata aaaaataaga gttgtgaaat tgaattaaaa gtacttccca
    10381 aaaaataccc acagggaagt gaaaaacatc taataaaatc aataactgga aaagaagttc
    10441 cctcaggtgg acttccaatt gatgttggaa ttgttgtttt taatgttcaa acagcaatat
    10501 caataaaaaa tgctgtctgt gatggagagc cacttattga aagagttata actgtttctg
    10561 gacttgttga aagacctaaa aatttaagag taaaaatcgg aacaagtata tccgaagtta
    10621 ttacttattg tgaaggtgaa ttagaagagg ggtctaagat tgtaattggt ggtcctatga
    10681 tggggatgaa tattccttct tctgatatac ctgtaatcaa gggaacaaca ggaattttaa
    10741 ttttaccaga agaaatttta ccagaagaaa ttgaaccatg tataagatgt ggaaaatgta
    10801 ttgatgcctg ccctatgaat ttatgtcctg ctgaaattgg aagatatgca gaattaaaaa
    10861 aatgggaaga agtagaaaaa ttaagtgttt ctgattgtat tgagtgtggt tgttgttcat
    10921 atatctgtcc ttctaaaagg ccattggttg aactatttaa atgggcaaaa acaaaaataa
    10981 agaggaaaaa agaatgaaaa aaataagttg ggcaccacat atacatagca atgaaacaac
    11041 aggcaaaatt atgttatctg ttattattgc cttaatacca cctttaattg gtgctatatg
    11101 gttttttggt ttatattcat tgaaaattat agttgtaagt attgtttcct gtgttttaag
    11161 tgaactttta agtcaaatta tttttaagaa aaaaataaga atttttgatg gaagtgcaat
    11221 tataacagga attttacttg cttttgtttt gccaccaaga atacctttat gggtggttgg
    11281 atttggtggc tttcttgcaa tttttcttat aaaagaaatt tttggtggaa ttggatttaa
    11341 tatatttaat cctgctcttg catcaagggc aatactcctc gcgtgttttc ctgtttttat
    11401 gactcactgg atacaaccat ttgactattc aatagatgca ataacgactg caactcctct
    11461 tacaatatta aaagaaaatt tcttacaaca attaccttca ttatggcaaa tgtttattgg
    11521 aataagggca gggtgtttgg gagaaacaag tgttttactt cttttattag gggcaatttt
    11581 tttgctttgt agaaaagtaa taacattgca tattccagtt tcatatatct taactgttgc
    11641 aattctttcg gtaattttca aacaaaatgt tgcaatgcag gttatggcag gaggacttat
    11701 tcttggtgct ttttttatgg caactgacta tgttacctcc cctataacaa aaaagggtaa
    11761 aataatattc ggtattggat gtggaattat aacttttctt atacgactga aaggaagtta
    11821 tccagaggga gtatgttatt caattttgtt tatgaatatg cttgttcctt taattgataa
    11881 atatactcta ccgaagaaat ttggaaggta aaaatgaaca aaaaagaaat agttggtggt
    11941 attttaattt taacaataat tacttcttta tccggatatt tacttgctta tgtttataaa
    12001 gtaacaaaac cagcaattga aaaacaacaa aaaattgaag aagataatat aaataaagaa
    12061 atttttccag atggagttga attccatgaa gtagaacaag gagatataaa atatatttct
    12121 gttttaggca gtgaaggaaa agaaataggt aaaattttta caacaacttc ccccggttat
    12181 ggaggagata taaaaataaa aattggagtt gatgaagatt taaaaattaa aggtgtaaaa
    12241 attcttcaac ataatgaaac acctggtctt ggagcaaaaa taactgaaaa aaaattttta
    12301 tcacagtttg atggtaaagg aaaagaaaaa atttatttaa aaaaggataa ttcagaaggt
    12361 gaaatagata caataacagg agcaacaatt tcatcaaaag cagtaacaga aggtgtgaga
    12421 aaattacttg aaacaattga ggtagaaaaa aatgaaaatt gattttaaga actttgtaaa
    12481 tgctttatgg aaagaaaatg cgatttttta tcttatgttg ggtttatgtc cttttctcgc
    12541 tgtttcaagt tctgttaggg attctattgg gatgggaatt gctgtaattt ttgttttagc
    12601 aggttctaat gtaaccattt ctttaataag aaaatttgtt ccctctcatg tgagaatccc
    12661 tgcatttatt gttgtaattg caacctttgt tacaattgtt gattattcac tcgctgcttt
    12721 atctcctcaa atccacagaa atttaggtgt ttatcttcca ctggtggttg ttaattgtat
    12781 tattcttggt agagcagagg catatgcatc aaaaaataat gtaatcagtt ctcttcttga
    12841 tggtttagga gtaggaattg gttttgctct tgtaattttt gttgtttcta ttgtaagaga
    12901 actttttgga aatggaacaa tacttggaca ccaaattttt ggagccaatt ttactccatt
    12961 tttaataatg attatgccac caggtgcttt tattgttatt ggttttttta ttgcgttaaa
    13021 acagggaatt gaaagaaggg gtaaaaaatg ttaaatcagg caaatttagg attaattttt
    13081 ctatctgcat ttttagtaaa taatattttg cttataaaat ttttagggct atgttctttt
    13141 tttggccttt caaaagatat taaatcatca ataggaatgg gacttgcagt aatttttgca
    13201 actgttatgg cttgttgttt aagttgggct gtgtacaatt tgcttctttt accactgcat
    13261 cttgaatatt taagaacagc aacttttatt ttaacaattg cttcatttgt ccagtttgaa
    13321 gaaataataa taaggaaaaa attgcctgtt ttatataagg catttggtat atatcttcct
    13381 ttaataacta caaattgtgc aattttagcg tgtgcttttc ttggagttga ttataaactt
    13441 tcttttatcc agaatcttgt tttctcaatc gctctaccag caggttatgc acttgcaata
    13501 attctttttg catctataag agagaaaatt gatgttgctc ctttaccaga agttttcaaa
    13561 ggtgctccaa tatcttttat aacagcgggt cttatgagtt tagcatttct tggttttaaa
    13621 gggcttttca aattataatc ttacaacata acaaataagg catctgcttt tcaaaatatt
    13681 tccagttggc cttattatat ctcaatcaaa aatttg
//
LOCUS       DAOOVW010000042        13036 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_3164_length_13036_cov_8.415145, whole genome shotgun sequence.
ACCESSION   DAOOVW010000042 DAOOVW010000000
VERSION     DAOOVW010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13036
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3164_length_13036_cov_8.415145"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..791
                     /locus_tag="PLW95_06280"
     CDS             72..791
                     /locus_tag="PLW95_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22269.1"
                     /translation="MNENNRELKSIFKETFNIKTNDIYLSLIVFLYIVISNLLSEINL
                     KVLSIVFIIQLFLLLLENYITISVYSGIKKIIKNQIIISKDIFIDGLYFFLRVLLYKI
                     FVCISFALLFAIAFGIIEVIKKIPVVNAAILTIITLLWLSIPVYYILLTFFAPFVIIL
                     EDRPVFDSMKISFNFVKENLDTFVIITFLFGILWSICILFFKKYNNLKGIEIFFFYIF
                     GLLEILTIKTFMILYNKIKVV"
     gene            1121..1714
                     /locus_tag="PLW95_06285"
     CDS             1121..1714
                     /locus_tag="PLW95_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOV22270.1"
                     /translation="MEQMFEIRWHGRGGQGAKTAALLFGEAAVSTGKYIQAFPEYGPE
                     RMGAPVSSFNRISSSPIRIHSGIENPDVVVVLDVSLIEQVNILDGLDPEKGILIVNTS
                     EKPEEIKKRLNFNGNVYTIDATKISIETIGRDIPNTPMMGALVKVTGILDIKELLEDT
                     KKKLEVKFRHKPEVIEGNLKAIERAYNEVVGIGSVKK"
     gene            1900..2190
                     /locus_tag="PLW95_06290"
     CDS             1900..2190
                     /locus_tag="PLW95_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOV22271.1"
                     /translation="MAEKNIGWKELKEGDVLEGATSLKFKTGNWRILKPCHIPEKCIH
                     CLFCWISCPEGAIIVDKENGKFVGFNYDYCKGCGICSYECPKEAIEMKMEEK"
     gene            2187..2450
                     /locus_tag="PLW95_06295"
     CDS             2187..2450
                     /locus_tag="PLW95_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22272.1"
                     /translation="MKKSLKLTFLILILSGWIAFGIQYIVFQQKTKNYEDKISQLNSE
                     INSYKMVSGDIKKSSEAINKIIDQLTNLKQNLENLQKNLDKPE"
     gene            2991..3782
                     /locus_tag="PLW95_06300"
     CDS             2991..3782
                     /locus_tag="PLW95_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOV22273.1"
                     /translation="MKKCRKGFTLIELLVVIAIIAILAAMLLPALARAREQARRGVCI
                     SNLKQIGLAIKMYAQDYDENCPGYDYNGAFDNDDCGPGQSLSLLVPKYAKDTGIFVCP
                     SSSDTKAPWWIDMDRGVIEGNYGTLTATAANLPLFGQHLSYAYAPGLNEQSRNESVLC
                     ADEIAYFGTSNTVVGDGGAWTDATNIRCQTADNHGIDGINVLYVDGHVAWVPTYKDGT
                     VYKLPVPKIGGGQIVADVSGGTDYEQIRNPVNGADENTPPTTQPF"
     gene            3834..4571
                     /locus_tag="PLW95_06305"
     CDS             3834..4571
                     /locus_tag="PLW95_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOV22274.1"
                     /translation="MKKRKGFTLIELLVVIAIIAILAAMLLPALARAREQARRSVCIS
                     NLKQIGLALRMYSQDYREFFPITSAGTGQTVPGCLGLLYPNYVSAQKTFVCPSDLSHV
                     TVGLATEESNQGISNVLVYVPGTTGNADDNGCSYAYALWCSEAVDIDTVLVVDKAVPT
                     SGTPAPGTAPGTDWSYTALKSGAATTSGGASATPVGLLNHKNDGVNACYVDGHVSWIP
                     VGKATKNDMFPNVDNAGGTQARVRNPY"
     gene            5345..7402
                     /locus_tag="PLW95_06310"
     CDS             5345..7402
                     /locus_tag="PLW95_06310"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22275.1"
                     /translation="MSIFLKGICGIIFQTILLREFFCSFFGSEFSFVIVLFFWMAGCG
                     IGSFLFKKVKNYSSPFNFLTIFENVFFILSIFLLRTLRHISQIQFFLPFIISSFFIVF
                     LSGLFEGSRFVLLSSLFKEEKSSGKVYGLEGLGALVGGIIIYFFFFLNSNPFFVLSII
                     SIVNLFTFIRPKKFSYFLLLPFLLILFYSNVLENISLNFKWKPFEIANSFYTPYQNIE
                     IVKKDKEIVLSTNGIPEISNQPDIYSLKNIVFFSICFLDKLENVLVVSNPEIIDEIEK
                     YKPKNIYYVDVDSKKIEIIKKNFLKKNYKNLIFINSQLDPFIKKTDLNFDVIFLGETF
                     PLNFYQNTRFTSSFVSHLSKKTKIVSLLLPGNYEYAGKDISQIHSIVYNTFKKHFEYS
                     KFIFTYPFISIFSNVQLNQVNKNFIYDNDFFNPSYIKYTLSHEKEKQYIRKNIIKTNE
                     NTINNQFLLLISVSNFLSLSNKKIGNFFFNYFKIISENKNILYFLLFFIFILFLLISR
                     KNVGSKIVFTNGFVSFGFESLLIIITQVIFGYVYSKISTLTALFMGGISIGSIISMNF
                     LKKENIFYIETFVLSFYILSFITIYFLRTISFPFLLGMIFISGFFVGLEFGLISFLDK
                     SQFVDKTGKLYAFDLLGGSLSSVVIPFLILPTFGLYLSFIIFPVVKIANFLSLRKNSR
                     YNE"
     gene            7722..8189
                     /locus_tag="PLW95_06315"
     CDS             7722..8189
                     /locus_tag="PLW95_06315"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOV22276.1"
                     /translation="MIREKRIKHNIFPLLKKLEEEIKSDEDVIFFYLFGSYGKNKISP
                     LSDIDFAFYLKEGIDYFDKKEELIEIINTVLKTDEVDVVILNEISPSFFKEILKTRKI
                     ILSKDANKRIEFELRKLREFFDTEKLRNFSEQALIRRIKERKYGIANSSKKSS"
     gene            8155..8571
                     /locus_tag="PLW95_06320"
     CDS             8155..8571
                     /locus_tag="PLW95_06320"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOV22277.1"
                     /translation="MELPIPVKNRLEKLEVALNKLDNLSKISLSEFLTDWKCQDSACR
                     NFQVAIEICLDIGTFLLSEKNLPIPDTYSLIIKELRENGIIDKNLEEDMKKLIKFRNI
                     IVHEYLYIDLEKVYKNFSYVDTFRKFADTIIKYFQV"
     gene            8658..9239
                     /gene="hisB"
                     /locus_tag="PLW95_06325"
     CDS             8658..9239
                     /gene="hisB"
                     /locus_tag="PLW95_06325"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOV22278.1"
                     /translation="MRKGKIIRKTKETDILIEINLDGNGNYEVKTDIGFLSHMLELFS
                     KFSGIDSKIKAKGDIEVDTHHLVEDVGICLGESIKQALGDKKGIRRFGFSSMPMDETL
                     VNISVDISGRPFLVFNVPDIRKKESVLEIEDIKEFFNGFVNHSGITLHINFVYGKNLH
                     HINEAIFKGVALSLKEAMKKEGKSTPSTKGRID"
     gene            9241..9561
                     /locus_tag="PLW95_06330"
     CDS             9241..9561
                     /locus_tag="PLW95_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22279.1"
                     /translation="MEENNLPNPRISALLSFVVNGLGQIYNGEIRKGLYLIFLSAFSL
                     LIFIIGSIFLFFFLWNGMTPFSFLVWGIILFFIGLIGIIILGIYSISDAYNTAKKMHQ
                     KGTE"
     gene            10094..10654
                     /locus_tag="PLW95_06335"
     CDS             10094..10654
                     /locus_tag="PLW95_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HOV22280.1"
                     /translation="MEKNIIIGVCGSISAYKSCEIVREFKKRNWEVKVIMTEKATKFI
                     SPLTLEVLSKNQVYVDMFDRKNYKQDHISLSEFADIILVAPATANIIGKIASGISDDL
                     LTCTIFAFSGTVIFAPGMNDNMWKNKIVEENVQKLKKYGYKFIGPEKGELASGKVGIG
                     RLAEIKKIIEITENEFKKQNNINEAI"
     gene            10641..12974
                     /locus_tag="PLW95_06340"
     CDS             10641..12974
                     /locus_tag="PLW95_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOV22281.1"
                     /translation="MKQYNFNSNLEKVALEKFGKKEKYPFERIKIIEVPNFSLLGKIV
                     ALRFIEWLQLNEEGVFSLPTGKTPEYFIEWASYYINNWNKKEIKEELKQWGLSEKKPN
                     LKNYYFVQIDEFYPINSERTNSFNYYIKNFYVKKFSLNPKKIILIDTWKVGCSPYKNL
                     SYIFPDEKVDLSLRYRKPKTELEKLQQKAIYQMDGFAMKYEEKIEQLGGIGFFLGGIG
                     PDGHIGFNIRGSDHNSTTRLLNINYETAAASSTDLGGIEVARGKCVVTIGLKTITQNP
                     TTTAIIMAAGESKAKVVMDAVENPPSILYPATSLQKLAGSRFYLTKGATSFLKGRKID
                     ELENKKLEKEDIEKIIIDISNKEKVKISNLDKNDFEDDIFGKILIKKNINIKKSLIIT
                     ENDIKRSIKKGTEVIKNTTFLHTSPHHDDIMLGYLPYILHLVRTPSNQHFFATFTSGF
                     TSVTNHYLLRKIQLLEKYLQENLLFTLLKEKDYFQPENINARNRDVFQYLDGIAANSK
                     EMIEEGESRRMLRNIVEITGKLDLEEIKRKIKKYKIYLINSYPGKKDIPEIQTLKGMV
                     REWEEELLWSHLGFNCNHIFHLRLPFYTGDIFTPEPEFPQDVKPVINLLEKLNPDIIT
                     VALDPEGTGPDTHYKVLQVVYQATKYYKEKYNKNFKIWGYRNIWFKFHPSECNIFVPV
                     SMNSLAIMKMAFDICFGSQKEASFPSYEYDGPFSELSQEIMVNQYSKIKTCLGRDYFY
                     SNKVPRLRATRGFVFIKEMEPEEFFKKAIELKRIMGF"
BASE COUNT         4728 a         1773 c         2259 g         4276 t
ORIGIN      
        1 attcgtcttc cccaaaagaa aagtagttta attataagat atgaagaaat ttgaactcta
       61 atatatttac aatgaatgaa aataatagag aattaaaatc aatttttaaa gagacattta
      121 atataaagac aaatgatatt tatttatcat tgatagtttt tttatatatt gtaataagca
      181 atttattgtc ggagattaat ttaaaagttc tatccatagt ttttataatt caactttttc
      241 ttttattact tgaaaactat ataacaatct ctgtttattc aggaataaaa aaaattatca
      301 aaaaccaaat tattatatcc aaagatattt ttattgatgg tctttatttt tttttaagag
      361 tccttcttta taaaattttt gtatgtattt catttgctct tctttttgct attgctttcg
      421 gaattattga agttattaaa aaaatacctg ttgttaatgc tgcgatttta accattataa
      481 cccttttatg gctttctatt cctgtttatt atatcctttt gacttttttt gctccttttg
      541 ttataatcct tgaagacaga cctgtttttg attcaatgaa aataagtttt aattttgtaa
      601 aagaaaattt agataccttt gtcattataa cttttttatt tgggattttg tggagtattt
      661 gtattttatt ttttaagaag tataataatt taaaaggaat tgaaattttc ttcttctaca
      721 tttttggttt gctggaaatt ttgacaatta aaacatttat gattttgtat aataaaataa
      781 aggtagttta agagggattt catccttctc cccgatgggg agaaggcggg gatgaggggg
      841 attgtaaaga agaaatccat cccctcgggc gtagtcctaa ggatccatag ccaaaagcga
      901 ggacccaacg ggcgagccca ccttcctttt taaaaggaag gagtaaaaaa catctcccct
      961 ctttgtaaag aggggtgggg ggagattttt tcttctcctt tgggggagaa ggttgatact
     1021 cgcccgtatg ggttatgccc gaggggcgag gggaattttt aaagtaaaaa tataaatatg
     1081 aacaaatcca ctgaatactt ataaaaaaag gaaaagaaaa atggaacaaa tgtttgaaat
     1141 aagatggcat ggaagaggag gacagggtgc aaaaacagcc gctttacttt ttggagaagc
     1201 agcggtctca acaggaaaat atattcaggc atttcctgaa tatggtcctg aaagaatggg
     1261 cgctcctgta tcttctttta ataggataag ttcatcacca ataagaatcc attcaggaat
     1321 tgaaaatcca gatgttgtgg ttgttcttga tgtaagttta attgaacagg taaatattct
     1381 tgatggctta gaccccgaaa aaggaatttt gatagtgaac acctctgaaa aaccagagga
     1441 aattaaaaaa agattaaatt ttaatggaaa tgtctatact attgatgcta caaaaatatc
     1501 aattgaaaca attggaagag atattcccaa tactccaatg atgggtgctc tcgtaaaagt
     1561 tacagggatt cttgatatta aggaattgct tgaagataca aagaaaaaat tagaagtaaa
     1621 attcagacat aaaccagaag ttattgaagg aaatttaaaa gcaattgaaa gagcatataa
     1681 tgaagtagtc ggtattggta gtgtcaaaaa atagatgaaa aaaagatgaa atcccccctc
     1741 accttaatcc tctccccagt ggggagaggc tcgcccgtag ggttcctcgc ttttggctat
     1801 ggatccctct ggactacgcc tgaggggtag gagtgaggga gtaataagca gataacaaaa
     1861 attttggcag taccttggta tttaaaagga gagagaaaaa tggctgaaaa aaacattgga
     1921 tggaaagaat taaaagaagg agatgtatta gaaggagcaa catctcttaa atttaaaaca
     1981 ggaaattgga gaatattaaa accatgccat attccagaaa aatgtattca ttgtttattt
     2041 tgctggatat cctgtccaga aggagcaatt attgttgata aggaaaatgg taaatttgtt
     2101 ggttttaatt atgattattg taagggatgc ggtatatgtt cctatgagtg tccaaaagaa
     2161 gcaattgaaa tgaaaatgga ggaaaaatga aaaaatcgtt aaagttgaca tttcttattt
     2221 taatactttc tggatggatt gcctttggaa ttcagtatat tgttttccaa caaaaaacaa
     2281 aaaattatga ggataaaata tctcaactaa attcagaaat aaattcatac aaaatggtct
     2341 caggtgatat aaaaaaaagt tcagaagcaa ttaataaaat tattgaccaa cttacaaact
     2401 taaaacagaa tttagaaaat ttacaaaaaa accttgacaa accagaataa aaatgttatt
     2461 gttaaaataa atttgacaaa ataaaaagtt gtgatataaa atatacttga caaaataaaa
     2521 aatatgttgt ataaataaag tgaaagagag aatccatccc tgcggtgagg gttttttcaa
     2581 aggaaggaaa aaacttctca cctttgacaa accgacaaaa gaggggattt gataaaatcc
     2641 atccctcggg cgtagtccga aggatccata gccaaaagcg aggaccctac gggcgagcca
     2701 accttccttt ttcaaaggaa ggagtaactt ctccctttat aaaaccgact aaggaagcaa
     2761 agggtagcag ggcagagttg gtgcgttatc gtctctcaga tagcacaacg gagattaaga
     2821 gggatttcat ccttatcccc agtctctgtc caacgagcat tttggcggag gggattgagg
     2881 gggatttaag aaagtaagca aaggaaaaac accttcactt tttctctttt cacaaaggga
     2941 gagaataaaa gtgaggggaa aaaaataaag aaaaaaggag gtgaaaaaaa atgaaaaaat
     3001 gcagaaaagg atttacattg attgaattgc tggtagtaat tgcgataata gcgattttag
     3061 cagcaatgtt actgccagca ttagcaagag caagagaaca ggcaaggaga ggtgtttgta
     3121 taagcaactt aaaacaaata ggacttgcta ttaaaatgta tgcacaggac tatgatgaga
     3181 attgccctgg ttatgattat aatggagcat ttgataatga tgactgtggt ccaggacaat
     3241 cactttctct tttagttcca aaatatgcaa aagatacagg gatatttgtt tgtccatctt
     3301 cttctgatac aaaagcacca tggtggattg atatggatag aggagttata gaaggaaatt
     3361 atggaacttt aacagcaaca gcggctaact tacctctttt tggtcaacat ctttcctatg
     3421 cttatgcacc tggattaaat gaacaaagta gaaatgaaag tgttctctgt gctgatgaaa
     3481 ttgcatactt tggtacaagt aatacagtgg taggtgatgg tggagcatgg acagacgcaa
     3541 ctaatatccg atgtcagaca gctgataatc acgggattga tggtataaat gttttatatg
     3601 tagatggaca tgtggcatgg gttccaactt ataaggatgg tactgtttat aaattaccag
     3661 tacctaaaat tggtggagga cagattgtag cagatgtaag tggtggtact gactatgaac
     3721 aaattaggaa cccggtcaat ggtgctgatg aaaatacacc acctactaca caaccattct
     3781 aagttagttg taattgttat ttgaaaaatt atataaaaag gaggtgaaaa aaaatgaaaa
     3841 agagaaaagg atttacatta attgaattgc tggtagtaat tgcgattata gcgattttag
     3901 cagcaatgtt actgccagca ttagcaagag caagagaaca agcaagaagg tctgtctgta
     3961 tatctaactt gaaacaaata gggcttgcat tgagaatgta tagccaggat tacagagaat
     4021 ttttccctat tacatcagca ggtacaggac agacagttcc gggttgttta ggacttcttt
     4081 atccaaacta tgtctctgct caaaaaacat ttgtatgccc atcagacctc tcccatgtga
     4141 ctgttggcct tgctactgaa gaatcaaacc agggaataag taatgtactg gtttatgttc
     4201 ctggaaccac tggaaatgct gatgacaatg ggtgttctta tgcttatgct ctctggtgtt
     4261 cagaagcagt tgatatagat acagtccttg tggttgataa agctgttcct actagtggta
     4321 ctcctgctcc tggaaccgcc cctggtactg actggagtta tactgcttta aagtcaggag
     4381 cggcaacaac atcaggtgga gcgagtgcaa caccggtagg acttcttaac cacaaaaatg
     4441 atggtgtaaa cgcatgctat gttgatgggc atgtatcatg gattccagtt ggtaaagcaa
     4501 caaaaaatga tatgttcccc aatgttgata atgctggtgg tactcaggca agggtaagga
     4561 atccttatta atcaaagaaa accaaaagaa gcccccgctt tttgcggggg cttctttttt
     4621 tttatcctgc cataatttca accaaagccc ttgtttttaa agaaaataaa atacccctaa
     4681 cccctaccct caatggctat tatcctttac ctacaagttt cccctttgaa atggttaaga
     4741 taaaagaaca tggttcttga aacaataaaa gcgagacata tatgcccctc tttataaaag
     4801 aggggcaagg ggagatttta ataaatccat ccctaccctt cctttacaaa ggaaggagtt
     4861 aatctcttcc ccctttttca aagggggtta gggggatttg gggattttca gatagcacaa
     4921 cgggagatag aggggagatt tcatccttct ccccgctggg gagaaggtgg ggatgagggg
     4981 gattgtaaag aagaaatcca ccccctgccc tctcctccac taaaacattg gcggtcatcc
     5041 ttttttaaag gagggagagt attttccccc tttaacaata cgactaaaga gggtaggatt
     5101 agcaagatag agttggtgcg tcatctcccc cttttattaa agcctgtccg acgcgcattt
     5161 tggcggagag atgaagaatg ttcccctctt tataaaagag gggcaagggg agattttaat
     5221 aaatccatcc ctaccttccc ttggaaaggg aaggagttaa aattccccct tttgtaaagg
     5281 gggatggagg aggattttgt gctttatcag taaagttcat tttactttta ccaaattatt
     5341 ataaatgtct atttttctta aaggtatttg tggaattatt tttcaaacaa ttcttttaag
     5401 agagtttttt tgtagtttct ttggtagtga attttccttt gttattgtcc ttttcttctg
     5461 gatggctggt tgtggaattg gtagtttctt attcaaaaaa gttaaaaatt attcttctcc
     5521 ttttaatttt ttaacaattt ttgaaaatgt tttttttatt ctctcaattt tccttttaag
     5581 aactttaaga catatttctc aaatacagtt ttttctacca tttattatat cctcattttt
     5641 tattgttttt ttatccggac tctttgaagg aagccgtttt gttcttcttt cttctctttt
     5701 taaagaagag aaaagtagcg gtaaagttta tggattagag ggattagggg cgttggttgg
     5761 tggaattata atttatttct tcttcttttt aaattctaat ccattttttg ttctttctat
     5821 aatatctatt gttaatcttt ttacttttat cagacctaaa aaattctctt attttttact
     5881 tcttcctttc ttacttattc ttttttattc caatgttctt gaaaacattt cattaaattt
     5941 taagtggaaa ccatttgaaa ttgccaactc attttatact ccatatcaaa atattgaaat
     6001 agttaaaaag gataaagaaa ttgtcctttc aacaaatgga atacctgaaa tttcaaatca
     6061 accagatatt tattctttaa aaaatatagt tttcttttca atatgttttc ttgataaatt
     6121 agaaaatgtc cttgttgtgt caaatccaga aataattgat gaaattgaaa aatataaacc
     6181 aaaaaatatt tattatgttg atgtagatag caaaaaaata gaaattatta aaaaaaattt
     6241 cttgaaaaaa aattataaaa acttgatatt tataaattca caattagacc cttttataaa
     6301 aaaaacagac ctgaattttg atgttatttt ccttggagag acatttcctt tgaactttta
     6361 tcaaaatact cggtttactt cttcttttgt ttctcatcta tcaaaaaaaa caaaaattgt
     6421 ttctttattg ctgccaggta actatgaata tgctggaaaa gatatttcac aaattcattc
     6481 aattgtctat aatacattta aaaaacattt tgaatattca aaatttattt ttacatatcc
     6541 tttcatttct atattttcaa atgtacaatt aaaccaagtt aataaaaatt ttatttatga
     6601 taatgatttt ttcaacccat catatataaa atacacttta agtcatgaaa aagaaaaaca
     6661 atatataagg aaaaatataa taaaaacaaa tgaaaataca attaacaatc aattcctcct
     6721 cttgatttca gtttccaact tcctttctct ttctaacaag aaaattggca atttcttttt
     6781 taattacttt aaaattattt cagagaataa aaatattttg tattttcttc tttttttcat
     6841 ttttatatta tttttactca tttcaagaaa aaatgttggt tcaaaaattg tttttacaaa
     6901 tggatttgta tcttttggat ttgaatcact tttaattatt attactcagg taatttttgg
     6961 ttatgtctat tcaaaaattt caactttaac tgctttattt atgggaggca tttcaatagg
     7021 gtccattatt tcaatgaatt tcttaaaaaa agaaaacata ttttatattg agacatttgt
     7081 tctttctttt tatattcttt catttattac aatttatttt ctaagaacaa tttcatttcc
     7141 ttttttactt ggtatgattt ttatttctgg tttttttgtt ggtcttgaat ttgggcttat
     7201 ttcttttctt gataaatccc aatttgtaga taaaacagga aaattatatg cttttgattt
     7261 gcttggtggt tctttatctt ctgttgttat accattttta attctaccta catttggtct
     7321 ttatctttct tttataatat ttcctgttgt taaaatagcc aacttccttt ctctaaggaa
     7381 aaattcaagg tacaatgaat aacaaagaca caaggtagaa agtagcaaag gtacaaagta
     7441 aaatactttg tcatcttata cttctttagg agttatccct tacccataag tcctccttta
     7501 caaaaggagg gagatgaaag aacagagtga attttgaaaa agatattcta aactttattg
     7561 tattatctga aaagtgataa agtaaagaga aaggtagtaa ttttctattt tataaaagaa
     7621 aataggctca aaggaaatcc ctttacccaa gaggatttta gaagatgtgg gtaagggata
     7681 tcttcaggag aagaaaggaa aaaaggggaa ggagagaaga gatgataaga gaaaagagaa
     7741 taaaacataa tattttccca ttgttgaaaa aattagagga agaaattaaa agtgatgaag
     7801 atgtaatatt cttttattta tttgggtcat atggtaaaaa taaaatttct cctttaagcg
     7861 atattgattt tgctttttat ttaaaagaag gcattgatta ttttgataaa aaagaagaac
     7921 taattgaaat aataaatact gtcctaaaaa cggatgaagt ggatgtcgtt atattaaatg
     7981 aaatttcccc atctttcttt aaggaaattt tgaaaacaag aaaaattatt ttgagtaaag
     8041 atgcaaataa aaggattgag tttgagttaa gaaaattaag agaatttttt gatacagaga
     8101 agttaagaaa tttttctgaa caagcactta ttagaagaat taaggagagg aaatatggaa
     8161 ttgccaattc cagtaaaaaa tcgtcttgaa aaattagaag ttgctttgaa taaattagat
     8221 aatttaagta aaatttcctt atcagaattt ctaactgact ggaaatgtca agatagtgca
     8281 tgcagaaatt ttcaggttgc aattgaaatt tgtcttgata ttggaacttt tttactatca
     8341 gaaaaaaatt tgcctattcc agatacatat tctttaatta ttaaagaatt aagagaaaat
     8401 ggaataattg ataaaaattt agaagaggat atgaaaaaat taataaagtt tagaaatatc
     8461 atagttcatg aatatttata tatagattta gaaaaagttt ataaaaattt ctcttatgtt
     8521 gatacattta gaaaattcgc tgatacaata ataaaatatt ttcaagtata atttttaaat
     8581 aaaaactctt ctacttttct aaccgccggg gttgaacaca attgacaatt agataaaaga
     8641 aaaagtaaaa tataaatatg agaaaaggga aaattataag aaaaacaaaa gagacagata
     8701 tattaataga aataaattta gatggcaatg gaaattatga agtaaaaaca gatattggat
     8761 ttttatctca tatgttagaa ttattttcaa aattttcagg aatagatagc aaaataaagg
     8821 caaaaggaga tattgaagtt gatactcatc accttgttga agatgttgga atatgtttag
     8881 gagaaagtat aaaacaagca ttgggagata aaaagggaat tagaagattt gggttttctt
     8941 caatgccaat ggatgaaaca ctagtgaata tatcagttga catttcaggt agaccttttc
     9001 ttgtttttaa tgttcctgat attagaaaaa aagagagtgt tttagaaatt gaagatataa
     9061 aggaattttt caatggtttt gtaaatcatt caggtattac ccttcatata aattttgttt
     9121 atggtaaaaa tcttcaccac ataaatgaag caatttttaa aggcgttgcc ttatctttaa
     9181 aagaagcaat gaaaaaagaa ggcaaaagca ccccatctac aaaaggaaga attgattaaa
     9241 atggaagaaa ataatttacc aaatccaaga atttctgccc tactttcttt tgttgtaaat
     9301 gggttaggac agatttataa tggtgaaata aggaagggat tatacttgat tttcctctct
     9361 gctttttctc ttttgatttt tataattggc tctatctttt tgtttttctt tttatggaat
     9421 ggaatgaccc ctttttcatt tcttgtctgg ggaattatat tattttttat tggtcttata
     9481 ggaataatta ttttaggaat atatagtatt tcagatgcat ataatacagc aaagaaaatg
     9541 caccaaaaag gaacagagta aaggaaaaat ccccctactc ttttttaata aaattgcgat
     9601 tagaagaaaa aatttgaaag agaaatttaa ttttcctctt tttttcttct ctccactccg
     9661 ccacaaagtt cgctacaaaa cattgcgaat ccgacactct ttccgccaaa aatcgtgtcg
     9721 gattcgaaat tctagccgaa tttggcggaa ttggcggaca gggacaggga agatgctcgc
     9781 ccgtgggcta tgcccgaggg gatggatttt catttatctt ttattaaatc cctctctttc
     9841 tacctttaat aaagggagac aactacactc cctcctttta taaaggaggg atggggtgga
     9901 tttagaaatc gcccttaaag tccccctgcc ccgttgtgcc tattgaaata cattacggca
     9961 ccaactctgt gtgctaaact ttgctacttt agtcggtttt acaaaggggg agatgacgca
    10021 ccaactctga gaaacagagt aagcaacttt aattggtttg gttagaggaa gaaattaaac
    10081 ggagttaata aaaatggaaa aaaatataat tattggtgtt tgtggttcaa tttctgctta
    10141 caaaagttgt gaaattgtaa gggaatttaa aaaaagaaat tgggaagtta aggtaattat
    10201 gacagaaaaa gcaacaaaat tcatttctcc attaactctt gaagtccttt ctaaaaatca
    10261 ggtctatgtt gatatgtttg acagaaaaaa ttataaacaa gaccatattt ctttaagcga
    10321 atttgcagat attattcttg ttgcccctgc aactgcaaat ataattggta aaattgcatc
    10381 aggtatatct gatgaccttc ttacctgtac tatttttgct ttttcaggta ctgttatatt
    10441 tgcacctggt atgaatgata atatgtggaa aaacaaaatt gttgaagaaa atgttcagaa
    10501 attaaaaaag tatggatata aatttattgg tcctgaaaaa ggagaacttg cttctgggaa
    10561 agttggaatt ggacgacttg cagaaataaa aaaaataatt gaaataaccg aaaatgagtt
    10621 taaaaaacaa aataatataa atgaagcaat ataattttaa ttcaaattta gaaaaagttg
    10681 cattagaaaa atttggtaaa aaagaaaaat acccatttga aagaattaaa ataattgaag
    10741 tcccaaattt ctcattattg ggaaaaattg ttgcattaag atttattgag tggctacaat
    10801 taaatgaaga aggagttttt tctctcccca caggaaaaac acctgaatat tttattgaat
    10861 gggcatcata ttacataaat aactggaata aaaaagagat aaaagaagaa ttaaagcagt
    10921 ggggactttc tgaaaaaaaa ccaaacttaa aaaattatta ttttgttcaa attgatgagt
    10981 tctatccaat aaattctgaa agaacaaata gtttcaatta ttatataaaa aatttctatg
    11041 ttaaaaaatt ttctttaaat ccaaaaaaaa taatccttat tgatacatgg aaagttggtt
    11101 gctctccata taaaaactta tcatatatat tcccggatga aaaagttgac ctttctttaa
    11161 gatatagaaa accaaaaaca gaacttgaaa aactacaaca aaaagcaata taccagatgg
    11221 atggatttgc tatgaaatat gaggagaaaa tagagcaact tggtggaatt ggattttttc
    11281 ttggtggaat tggtcctgat ggtcatattg gttttaatat aagaggttct gaccataatt
    11341 caacaactcg tctcttaaac ataaattatg aaaccgcagc agcaagttct accgaccttg
    11401 gaggaattga agttgcgagg ggaaaatgtg tagttacaat tggactaaaa acaataactc
    11461 aaaatccaac tacaacagca ataataatgg cagcgggtga aagtaaagca aaagtcgtaa
    11521 tggatgcagt ggaaaatcca ccttcaattt tatatcctgc aacttcctta cagaaactgg
    11581 caggaagtag attttattta acaaaaggcg ctacatcttt tttaaaagga agaaaaattg
    11641 atgaacttga aaataaaaaa ttagaaaaag aagatattga aaaaatcata attgatatat
    11701 ccaataaaga aaaagtaaaa attagtaatt tagataaaaa tgattttgaa gatgatattt
    11761 tcgggaaaat tttaattaag aaaaatataa atataaaaaa atctttaatt ataactgaaa
    11821 atgatataaa aagaagcata aaaaagggaa cagaagtaat caaaaataca acatttcttc
    11881 atacttcccc acatcatgat gatataatgc ttggatatct tccttatata cttcatcttg
    11941 tccgaactcc ttcaaatcaa catttttttg ctacatttac aagtggtttt acttctgtta
    12001 caaatcatta tcttttgaga aaaattcaat tacttgaaaa atatttacag gaaaatttac
    12061 tttttactct tttaaaagag aaggactatt ttcaaccaga aaatataaat gcaagaaata
    12121 gagatgtatt tcaatattta gatggaatag cagcaaatag taaagaaatg attgaagaag
    12181 gtgaaagcag aaggatgtta agaaatattg tagaaataac aggaaaattg gatttagaag
    12241 aaataaaaag aaaaataaaa aaatataaaa tatatttaat aaattcatat ccaggcaaaa
    12301 aagatattcc tgaaatacag acattaaaag gaatggtaag agaatgggaa gaagaacttt
    12361 tgtggtccca tttaggtttt aactgcaacc atatttttca tcttcgtctt ccattttata
    12421 caggtgatat atttactcct gaaccagaat tcccacagga tgtaaagccg gttattaacc
    12481 tattagaaaa attaaatcca gatataatta cagttgcttt agaccctgaa gggacaggtc
    12541 ctgacacgca ttataaggtc ttacaggttg tttatcaggc aacaaaatat tataaagaaa
    12601 aatataacaa gaattttaaa atatggggtt atagaaatat atggtttaag tttcatcctt
    12661 ctgaatgtaa tatctttgtt cctgtttcta tgaactcact tgctattatg aaaatggctt
    12721 ttgatatatg ttttggttca caaaaagaag catctttccc atcttatgaa tatgatggtc
    12781 ctttttctga actttctcag gaaatcatgg taaatcaata ctcaaaaata aaaacatgtc
    12841 ttggtaggga ttatttttat tcaaacaaag ttccacgcct aagagcaaca agaggatttg
    12901 ttttcataaa agagatggaa ccagaagaat tctttaagaa agcaattgaa ctcaaacgaa
    12961 ttatgggctt ctgaccctcc tttatattga cttttaagaa atgcaagttt aaaatttatt
    13021 ataaaagtaa catagt
//
LOCUS       DAOOVW010000043        12743 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_3245_length_12743_cov_7.345602, whole genome shotgun sequence.
ACCESSION   DAOOVW010000043 DAOOVW010000000
VERSION     DAOOVW010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12743
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3245_length_12743_cov_7.345602"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(384..1424)
                     /gene="prfA"
                     /locus_tag="PLW95_06345"
     CDS             complement(384..1424)
                     /gene="prfA"
                     /locus_tag="PLW95_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOV22282.1"
                     /translation="MKVSEKLKELEEEYKKNQSIISNAKVGDKKYIAAIKKVGELEEI
                     LKYKRQLDILKEKIKETESIVENDEQELKVLVEEEIEKLKKEKEEIETKIEEYLSPEK
                     AQNKKNTIIEIRGGVGGEEAYLFARDLFRMYSKFCEKKNYKIEVLSLNKTELGGIKEI
                     IFLVTGNGAYGDFKYESGVHRVQRIPETENYGRIHTSAATVSVFPEIEEGEIKIDPND
                     LKIETFRASGHGGQHVNKTSSAVRITYIPTGISASCQDERSQFKNKSKAMKILKARLQ
                     KIYEEEKKSKIDEERKKQIKTGERSEKIRTYNFPQNRLTDHRINLTLYNLNDIIEGDM
                     EKLITTLRQELK"
     gene            complement(1411..1611)
                     /gene="rpmE"
                     /locus_tag="PLW95_06350"
     CDS             complement(1411..1611)
                     /gene="rpmE"
                     /locus_tag="PLW95_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013626279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOV22283.1"
                     /translation="MREKIHPKYEEATITCVCGSVVKTMSTKKDIKVDICSNCHPYFT
                     GKQKFVDSAGRVEKFKKKYESK"
     gene            complement(1583..2152)
                     /locus_tag="PLW95_06355"
     CDS             complement(1583..2152)
                     /locus_tag="PLW95_06355"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOV22284.1"
                     /translation="MEKSEKIILILLLIIFVFLICVLGFIKIGKKFKGGYVYFVKSDT
                     EDTYLVKVERKLPLGSSVEKKIEVLIENLLIGPTEKEERIGISTTLPEGVKLHKVERN
                     GDTVYIDFSEEIEQGGGTSLMETRLAQIVFTATQFPDVKKVKLLIDGKEIEYFSSEGI
                     MVDKPLTKDDFVEFIKEEEENERKNTSKI"
     gene            complement(2620..3924)
                     /locus_tag="PLW95_06360"
     CDS             complement(2620..3924)
                     /locus_tag="PLW95_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOV22285.1"
                     /translation="MIKEILKKIERKEIKIGVIGLGYVGLPVVLRFARVGFEVVGFDT
                     DQEKVNKINKGISYISHIPSEYIKKVLSRFTATTDITKVKEVDAIIICVPTPLDKYKQ
                     PDIKYLEEVSSDIYKNIKKGHIISLESTTYPSTTREIFNSKFEKTNLKVGNDYFLIYS
                     PERENPGLDPFEGRKIPKVVGGITENCTKVGVSLYSHVIEKVIPVSSTEVAEATKLLE
                     NIYRAVNIALVNELKMLFEKMGINIWEVIEAAKTKPFGFQPFYPGPGLGGHCIPIDPF
                     YLTWKAKEYDFSTRFIELAGEINTSMPYYVVEKCIEALNKKGISIKEGKILLIGIAYK
                     KDVDDIRESPTIKILKILKEKGFKKVDYYDPYVKEVKKTRQFPHAIKSISFSSSILKK
                     YNAGIVVTDHSNIDYELLSKILPVIVDTRNVYKDKKKNVFKA"
     gene            complement(4143..6731)
                     /locus_tag="PLW95_06365"
     CDS             complement(4143..6731)
                     /locus_tag="PLW95_06365"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOV22286.1"
                     /translation="MKEKFFIANIIEASAGTGKTRYITEEMLSIYREYRNPEILKRIV
                     AITFSEKAAIEMKERFLKKFYGEIFSDLSEKEKVEIENVLFKLQISTIHSYCQTLLRR
                     FYFLSDIDPYFEIIQEGESGVLFNRAIYKVLNTKSGRKSFAEISKKFKLNHFKNVLFS
                     FLNAHPHIFLGEPEGEITKKITSFYNIISTQHFFLKKEFSYLDFDDLEKLTYKTLRDN
                     QDALVVLEDFDENINFIFIDEFQDTNLLQFEIVKKLIEEWISGYGAKVDRGENFGIII
                     VGDRKQSIYKFRGAESKLFDDAKEVLSAYMTEKVLTENYRSSKEIIDFVNNVFENESP
                     WNIQKLIVSEKKFNLPSKIEIKLFDNKNEEEKKNEYKWVCQKIIEIVENKIPVIENDI
                     ERTIEFKDIAILLRQRKGKYFKYLERYLSDFGIPYVIIGGVGFYQEEEIIMLTSLLFA
                     LVDPEDKFSIWNIENSILYMDKREIEKLRQYLGQYNIVEIIENIFKEKNIWEKLNGQQ
                     KANCEKFLMILNQQKKLPLFQLTQNLREISERTEEPKADIFSIHQNAVKINTIHGSKG
                     LEFPCVFLINIEDGGSSRKNDEIIYIREKGIYKYSLNIESQENFKDIYKREIKEEEKR
                     LLYVALTRSSQYLFISGQKSKNLWVNMIEKTEEKYKAEEITRKSRKIEKEKIKKEKIE
                     IPIKFTPLTSYTKQIERTEETSDMIFGEVIHKVLSEISNNKILFSENNFTGRCEFYLK
                     KKINDWDKYNKKIKDIFKKICDNDKIKEIITYKNGLSEVPFVYKEKKQIFEGKIDKVI
                     IEKKKCKVYDYKLNIKNPNRYMKQLNIYEKAVKKIFKVGKVEKYLISLEEGEIYNCSE
                     RHKYIE"
     gene            complement(6728..9340)
                     /locus_tag="PLW95_06370"
     CDS             complement(6728..9340)
                     /locus_tag="PLW95_06370"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOV22287.1"
                     /translation="MKKLIIFPFSYRGKLEYILKNFIKEEEKTILIYSNFSKIKEVKK
                     LYSNFLQKNLLPEILTIKTLACQLVEENSEKDIVDELERFTIILKIINKAGKLQDISI
                     AGMGEGINNLIKDLKTSFEITPDYEIILKEINNYPWRFEKNKNDLLFAFNVIRTYENY
                     LKEKNLIDIDDLYKEGEKYISQIKKERLIIENFYEILPSQKNFFSSLINYFPDVIFSF
                     SYDENVSPDVKELILDKTLSFIKNITDWEIIKMETEENKSEVECYNFPSQDEEIKGIV
                     KLISENFKKNKSLTIDDFSITCPDMLGYRDTIERIFNRYGIPSEMVPGYTLNNEPSII
                     SLLDILIFIQTYDWKTLMGVLSSPYFKKINFEDFKKFSSYTRDNYEGIGFTKENFYKS
                     NNTAVLLIKECVEIIKARENTLDNWVKNLLKIMEKLGWQPQNSEIERKFKEVLGYMKG
                     DFSLTSSQFLNILKKCLEFKEIEESRGDGLRVSGVIESTGMEKKISFISGATEENIPN
                     APKLLEIFLPDKLKGKIGISDYKLRIARDRLDIHRLLNENEKVIFTYPSKIEGRNQMK
                     SIFLMNFDEKTIYEKEINSSQTKIFNFGFSNEKFYEKYLKNGKIEIGVTSLEEFLKCK
                     YRFYLKNVENIKPYIIPEIEEVPEIWGKMVHTIMENIFNIYKGKILSGKFIEDVVKQF
                     KSQLFSEIKNYTNSEISEFYRDIMKIRAKEVVEKFKKIIVNHIGNKLLELEFEIPYEG
                     PTYILKGKIDRIEENNDGLYIIDIKTGTSLPPSYTENDFEKNKNIQIPAYIWMFKNKF
                     NLKKDVYGAIWNFSFKEDDKNKEEKKYNLTYIDRMDEYFNNIFDEIIKGKINFQPEEN
                     PSCFYCEFKTQCPK"
     gene            9387..10295
                     /locus_tag="PLW95_06375"
     CDS             9387..10295
                     /locus_tag="PLW95_06375"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22288.1"
                     /translation="MRKKEKIIGKVCFRFIVYYLFLQVYYITYFLPTIYYKYWAILWG
                     NFAYLTMSNSKKRIIINLSIAFKDKLSYKDKKKITKEFFYQIVLNFIELVKITKIPMS
                     EYEKMIEIEGEEILKKVVAEKKGVVAVCCHLGNFPVVQAYLAKKNYPISMIIRYSNNI
                     FLSRFNDRLLKKLKVPFVSKWDLKEAIEYSKKYLKKGGIVCFYLDQHDGKGVKVDFFG
                     EKVFTPTGAAVFARKYKCNVIGIFTYRTKKNKHKVIIEGPYKTEYTANTRSDIENMSA
                     FFIKRVEYYVEKHPEQWFTWLHRRFR"
     gene            complement(10275..11204)
                     /locus_tag="PLW95_06380"
     CDS             complement(10275..11204)
                     /locus_tag="PLW95_06380"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOV22289.1"
                     /translation="MKIGLALGGGGALSLSQIGVIKVFEREGIEFDIITGVSMGSVVG
                     SLYSFTKDSKQLEEFFKRFINDRNIKKIEEIFIRHRKRGLTEKITDGFKNLYILFSDS
                     FKTGLFDSEKVIEEVRKIFNEEIYIENSKINLGIVAVEYFTGKIFIFNRGKLFPAVVA
                     SCAMPGLLTLVEINGKKFIDGGVASNIPVISNYILGGEIIICVENEPSLLKNRPSNVF
                     STLVQVSKIKSKYADILEEIYADFKIEIELEGIEWYNFSQFQYCVKEGEKTAEKYLPY
                     IEKVLRQEKTPISEIREKIIKNIKGNFLTESSM"
     gene            complement(11201..11779)
                     /locus_tag="PLW95_06385"
     CDS             complement(11201..11779)
                     /locus_tag="PLW95_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22290.1"
                     /translation="MWKRKIYRIVMGSIAPFFYILTKDIFLPLAISTFFLTLLGALEF
                     ERWKNPSVWKWMTEKYGGVFKKKVGRFTGDTYFMISNFLVLLFFPYDIAIYSLFFLTF
                     EDAGSGIIGKKYGKREIFKGKTLEGLIGGILFNLAIGFLLLFFVEIPYPVFFTGVFLS
                     AILEILPIPVDDNLSVGLLTPIFMFIVYKVVL"
     gene            complement(11757..12359)
                     /locus_tag="PLW95_06390"
     CDS             complement(11757..12359)
                     /locus_tag="PLW95_06390"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HOV22291.1"
                     /translation="MRIVFLFFISYLCGSIPFAFLFTYFLIGKDIRKIGDGNPGAANV
                     VRNVSKKWGIMVWFCDTIKGAFPIFLGKCWGETNIIFLTIIGCFAIIGHCYSVFLKFK
                     GGKGAATSGGVILSLMPSLFPLVIILWFTAQKINPRSPKILLSCIFIFLLFVFFIYKE
                     QFIYYFISTLILILTGAIINKNIIKEIRGKKIGNVEKKNL"
BASE COUNT         4200 a         2141 c         1400 g         5002 t
ORIGIN      
        1 ccttccttta tcaaaggaag ggatggattt tatattaaat cttcccttgc cccctttgtc
       61 aaagtgggaa atatcccccc tcatccccac cctcgggcgt agccctacgg gcgagccttc
      121 tccccgatgg ggagaaggat gaaattcctc tctatccccg ttgtgcttgg aaatccccta
      181 aatcccctct atctcccctt taataaaggg gagaataagc accaactctg ccctgctaat
      241 ccctgcaact tttgtcggta gcagttgtgc tatctgggag acgataacgc accaactctg
      301 ctttgctacc ctttgcttcc ttagtcggtt tgttaaaggg gagaattaaa aagggggaac
      361 aaaaagaaaa aatttataaa tttttatttt aattcctgcc tcaatgtagt tatcaacttt
      421 tccatatcac cttctattat gtcattaagg ttataaagag tcaaatttat tctgtggtca
      481 gtaagacgat tttgagggaa attgtaagtt cttatttttt cacttctttc ccctgtttta
      541 atttgttttt tcctttcttc gtctatttta ctttttttct cttcttcgta aattttttgt
      601 aatcttgctt ttaaaatttt cattgcctta cttttatttt taaactgaga cctttcgtcc
      661 tgacaacttg cagaaatacc ggttggtata taagttattc tcacagcaga agatgttttg
      721 ttgacatgct gtcctccatg acctgaggcg cgaaatgtct caattttcaa atcattaggg
      781 tcaattttaa tttctccttc ttctatttcg ggaaaaacag atacagttgc cgctgaggtg
      841 tgaattcttc cataattttc tgtttcaggt attctttgga ccctatgaac cccactttca
      901 tatttgaaat caccatatgc accattccca gtaacaagaa aaataatttc ttttatgcca
      961 cctaactctg tcttatttaa agaaagaact tcaattttat aatttttctt ttcgcagaat
     1021 ttagaataca ttctaaaaag gtctctggca aaaaggtatg cttcttctcc cccaactcct
     1081 cctcttattt caattatagt atttttttta ttctgggctt tttctgggga aagatattct
     1141 tcaatttttg tttctatttc ttccttctct ttttttagct tctctatttc ctcttctacc
     1201 agaactttta actcctgctc atcattttca acaatacttt ctgtttcttt aattttttct
     1261 ttaagtatat ccaattgcct cttgtatttt aaaatttctt caagttctcc tacttttttt
     1321 attgcagcaa tatatttttt atcaccaact ttcgcattag aaataatcga ctgattcttt
     1381 ttatactcct cttccaattc ctttaatttt tcacttactt tcatatttct ttttaaactt
     1441 ctccactcta ccagcactat caacaaattt ctgttttcct gtaaaatatg gatggcaatt
     1501 tgaacatata tcaactttta tatctttttt agttgacatt gtcttaacaa cacttccaca
     1561 aacacaggta attgttgctt cttcatattt tggatgtatt ttttctctca ttttcttcct
     1621 cctccttaat aaattcgaca aaatcatctt ttgttaaagg tttatctacc attattcctt
     1681 cactgctaaa atactctatt tctttcccat caataagaag tttaactttt ttaacatcag
     1741 gaaattgagt agcagtaaaa acaatctggg ctaatcttgt ttccatcaaa gaagtccctc
     1801 ctccctgctc tatttcttcg gaaaaatcaa tataaacagt atctccattt ctttctactt
     1861 tatgaagttt tacaccttcg ggtaaagttg ttgaaatgcc tatcctttct tccttttcag
     1921 ttggaccaat taataaattc tctataagaa cctctatttt tttttcaacg ctgcttccta
     1981 atggaagttt cctttccact ttcactaaat aagtatcctc tgtatcactt ttcacaaaat
     2041 atacatatcc acctttaaat tttttcccga ttttaataaa tccaagtaca caaattaaaa
     2101 aaacaaaaat aataagtaat aaaattaaaa taattttttc gcttttttcc ataatacaat
     2161 tatattcttg aaggagaaaa aagtcaatct ttataagtat tcagaaaaga gtattatatt
     2221 aatgcacaat tctcctaacc cgttgtattt gttaaattcc ccttcatccc tctttgttaa
     2281 atgggagaca tttgtctcct tcctttatca aaggaaggtg gggatggatt ttattaaact
     2341 cacatcattc ccgttgtgct atctggtaga cgataatgca ccaactctgc tttgctactc
     2401 cccgctactt tagtcggttt ttcaaatctc cccctcatcc ctaccttctc cccgttgggg
     2461 agaaggatga aatctcccta tatcctctct gccacaaatc acgacggaca gatgcttttt
     2521 caaaggggga atggggaaat tttgtctttg tttttatctt atcacaaggt tccatcggac
     2581 tacttgccat ttctttaagc aacataagtt gattgaaaat tatgctttaa aaacattttt
     2641 ctttttatct ttgtagacat ttcttgtgtc aactataact ggtaaaattt ttgaaagtaa
     2701 ttcataatca atatttgaat ggtcagtaac aacaattcct gcattatatt tttttaaaat
     2761 agaagaagaa aaagaaattg attttatagc atgaggaaat tgtcttgttt tctttacttc
     2821 tttaacatag gggtcataat aatcaacttt tttaaaaccc ttctctttta atatttttaa
     2881 aattttaata gtaggagatt ccctgatatc atcaacatct tttttatatg caattccaat
     2941 taaaagaatt ttaccttctt ttattgaaat tccttttttg tttaatgctt ctatacattt
     3001 ctcaactaca taatatggca ttgatgtatt tatctctcca gcaagttcaa taaaccttgt
     3061 agaaaaatca tattcttttg ctttccatgt taaataaaat gggtctatgg gaatacaatg
     3121 acctccaagc ccaggaccag gataaaatgg ctgaaaacca aatggctttg tttttgctgc
     3181 ctcaattact tcccatatgt ttattcccat tttttcaaat aacattttta attcattgac
     3241 aagagcaata ttaactgccc tatagatatt ttcaagtaat tttgttgctt ctgcaacttc
     3301 tgttgaagaa acaggaataa ctttttcgat aacatgagaa taaagagata caccaacttt
     3361 tgtgcaattt tcagttattc ctccaacaac ctttggtatt tttctgcctt caaatggatc
     3421 taatccggga ttttctcttt cgggtgaata aataagaaaa tagtcatttc caacttttaa
     3481 attagttttt tcaaattttg aattaaaaat ttcccttgtt gtactcggat aagttgttga
     3541 ttcaagagag ataatatgac ctttttttat atttttataa atatcagatg aaacctcttc
     3601 aagatattta atatctggtt gtttatattt atcaagtgga gttggaacac atataattat
     3661 tgcatcaact tcttttactt ttgttatatc agtagtagca gtaaatcttg acagaacctt
     3721 tttgatatat tcagaaggga tatgagaaat gtaagaaatc cctttattta ttttattaac
     3781 tttttcctgg tcagtatcaa aaccaacaac ctcaaaccca actcttgcaa atcttaaaac
     3841 aacaggtagt cccacataac caagtcctat aactccaatt tttatttctt ttctttctat
     3901 cttttttaaa atttctttta tcataatttt ccctttatta tttaataaaa attatattac
     3961 aacaaaaaac aaggttttta aaaatccatc ccgaccttcc ctcggccgta gcctgtaggc
     4021 tacagcctac gggcgaggcc tttttcaaag gaaggagtat atcccctctt tgaaaaagag
     4081 gggaagggga gattttactt ttaaaaaact ctgtgcttta ttgcttcttc ctttaaattc
     4141 tctcactcta tatatttgtg cctttctgag caattataaa tttccccttc ttccaatgat
     4201 attaaatact tttcaacctt acctacttta aaaatctttt taactgcttt ttcatatata
     4261 ttaagttgtt tcatatacct atttggattt ttaatattta atttataatc atacacctta
     4321 catttctttt tttcaataat tactttatca attttgcctt caaaaatttg ttttttttct
     4381 ttataaacga aaggaacttc tgatagacca tttttataag taataatttc ttttatttta
     4441 tcattatcac atattttttt aaatatatct tttatttttt tattatattt atcccaatcg
     4501 tttatttttt tctttaaata aaattcacat ctaccagtaa aattattttc agaaaaaaga
     4561 attttattat ttgatatttc acttagtact ttatgaatta cttctccaaa aatcatatca
     4621 gatgtttctt ctgttctttc tatctgtttt gtataagaag ttagtggagt aaattttata
     4681 ggtatttcaa ttttttcttt ttttattttt tccttttcaa tttttcttga ctttcttgtt
     4741 atttcttctg ctttatattt ttcttcagtt ttttcaatca tatttaccca caaattttta
     4801 cttttttgcc cagaaataaa tagatactga gaagaccttg taagagcaac ataaagtaat
     4861 cttttttctt cttcttttat ttctctctta tatatatctt taaaattctc ctggctttca
     4921 atatttaaag aatatttata aatacctttc tcccttatgt aaataatttc atcgttcttt
     4981 ctgctactgc caccatcttc aatatttatt aaaaaaacac acggaaattc aagtccttta
     5041 cttccgtgaa tagtattaat ctttacagcg ttttgatgaa ttgagaaaat atctgctttt
     5101 ggttcctcag ttctttctga aatttctctt aaattctgtg ttaattgaaa aagaggcaat
     5161 tttttttgtt ggtttaaaat cattaaaaat ttctcacaat tggctttttg ttgtccattt
     5221 aatttttccc atatattttt ttctttaaag atattttcaa ttatttcaac aatattgtat
     5281 tgacctaaat attgcctcaa tttttctatt tctcttttat ccatatataa aatggaattt
     5341 tctatgttcc agatagaaaa tttatcttct gggtcaacca aagcaaataa taaagaagta
     5401 agcataataa tttcttcttc ctgataaaaa ccaacaccac caataataac atatggaata
     5461 ccaaagtcag aaagatacct ttccaaatac ttaaaatatt tgccttttct ttgtcttaaa
     5521 agaatagcaa tatctttaaa ctctattgtt ctttctatat cattttctat tacaggaatt
     5581 ttattttcta ctatttcaat aattttttga catacccact tatactcatt tttcttttcc
     5641 tcttcatttt tattatcaaa aagttttatt tctatttttg atggtaaatt aaactttttc
     5701 tctgaaacaa taagtttctg aatattccac ggactttcat tttcaaacac attattaaca
     5761 aaatcaatta tttccttact actcctataa ttctctgtta aaactttttc agtcatatag
     5821 gcagaaagca cttcctttgc atcgtcaaat aatttacttt ctgctcctct aaatttatag
     5881 attgactgct ttctatctcc aactataata attccaaaat tttctcctct atcaactttt
     5941 gctccataac cagaaatcca ttcttcaatc agttttttta caatttcaaa ttgaagaaga
     6001 tttgtatcct gaaattcatc aataaaaata aagtttatat tctcatcaaa atcttcaaga
     6061 actaccaatg catcttgatt atctcttaat gttttataag taagtttttc aaggtcatca
     6121 aaatcaagat atgaaaattc ctttttaaga aaaaaatgtt gtgttgaaat aatattataa
     6181 aaagaagtta ttttttttgt aatttctcct tctggttctc caagaaaaat atgtggatgt
     6241 gcatttaaga aagaaaacag aacatttttg aaatggttta atttaaattt ctttgaaatt
     6301 tctgcaaaac tttttctccc actttttgta tttaatactt tataaattgc cctattaaaa
     6361 agcactccac tttcaccttc ctgaattatt tcaaaataag ggtcaatatc acttaaaaaa
     6421 taaaatcttc ttaaaagtgt ttgacaatat gaatgaattg ttgatatttg taacttaaat
     6481 aaaacatttt caatttcaac tttttctttt tcacttaaat cgctaaaaat ttcaccataa
     6541 aatttcttta aaaatctttc cttcatttca attgctgctt tttctgaaaa agtaatagca
     6601 acaattcttt ttaaaatctc tggatttcta tattccctgt aaatagaaag catttcttct
     6661 gttatgtatc tggtctttcc tgttcctgct gatgcttcta ttatattggc tataaaaaat
     6721 ttctctttca ttttggacac tgggttttaa attcacaata aaaacaggat ggattttctt
     6781 ctggttgaaa atttattttc cctttaatta tttcatcaaa aatattatta aaatattcat
     6841 ccattctgtc aatataggtt aaattatact ttttttcttc tttgtttttg tcatcttctt
     6901 taaaagaaaa attccatatt gcgccataaa catctttttt taaattaaat ttatttttga
     6961 acatccatat atatgcggga atctggatat tcttattttt ttcaaaatca ttttctgtat
     7021 aacttggggg taatgatgtt cctgttttaa tatcaattat gtaaagacca tcattatttt
     7081 cttcaattct atcaattttc ccttttaaaa tataagttgg tccttcataa ggaatttcaa
     7141 attcaagttc aagaagttta tttccaatat gatttacaat tattttttta aatttttcaa
     7201 ctacttcttt tgcccttatt ttcattatat ctctgtaaaa ctctgaaatt tctgaatttg
     7261 tataattttt tatttcagaa aagagttgac tcttaaattg tttgacaaca tcttcaataa
     7321 atttacctga caaaattttc cctttatata tattaaaaat attttccata attgtatgaa
     7381 ccattttccc ccatatttct ggaacttctt caatttcagg aattatatat ggtttgatat
     7441 tttccacatt ttttaaataa aacctatact tacattttaa aaattcttca agtgaagtaa
     7501 ccccaatttc tattttcccg tttttcaaat acttttcata aaatttctca tttgaaaaac
     7561 caaaattaaa aatttttgtt tgagaagaat ttatttcttt ttcataaatt gttttttcat
     7621 caaaattcat aagaaaaatt gatttcatct ggtttctacc ctcaattttg gatggatatg
     7681 taaatataac tttttcattt tcatttaaaa gtcggtgaat atcaagtcgg tctctggcaa
     7741 ttcttaattt ataatcagaa ataccaattt taccttttaa tttatcaggt aaaaatatct
     7801 caagcaattt tggagcatta ggaatatttt cttctgttgc ccctgaaata aaagaaattt
     7861 ttttctccat tcctgtactt tcaattactc cagaaaccct taacccatct cccctgctct
     7921 cttctatttc tttaaattct aaacattttt tcagtatatt taaaaattgt gaagaagtta
     7981 gagaaaaatc tcctttcata tatccaagaa cttctttaaa ttttctttct atttcactgt
     8041 tttgtggttg ccagcccaat ttctccataa tttttaaaag atttttcacc cagttatcca
     8101 gagtattttc ccttgctttt ataatttcaa cacactcttt gataagaaga acagcagtgt
     8161 tattgctttt ataaaaattt tcttttgtaa aaccaattcc ttcataatta tctcttgtat
     8221 aggatgaaaa ttttttaaaa tcttcaaagt taattttctt aaaataggga gaagataaaa
     8281 ctcccatgag tgttttccaa tcatatgtct gtataaaaat tagaatatct aaaagagaaa
     8341 taatagatgg ctcattattt aatgtataac cagggaccat ttcagatgga attccatatc
     8401 tattaaatat tctttcaata gtatctctat agcccaacat atcagggcag gtaattgaaa
     8461 aatcatcaat tgtcaaggat ttgttttttt taaaattttc tgaaattaac ttaacaattc
     8521 cttttatttc ttcatcttga gatgggaaat tataacattc aacctcactt ttgttttcct
     8581 cagtttccat cttaattatc tcccaatcag ttatattttt aataaaacta agtgttttat
     8641 caagaattaa ttctttaaca tctggtgaga cattttcatc ataagaaaaa gaaaaaataa
     8701 catcaggaaa ataatttata agagaagaaa agaagttttt ttgagaaggt aaaatttcgt
     8761 aaaagttttc tattatcaat ctttcctttt taatttgact aatatatttt tcaccttctt
     8821 tataaaggtc atctatatca attaaatttt tttcttttag ataattttca taagttctta
     8881 ttacattaaa agcaaataat aaatcatttt tattcttttc aaatctccat ggataattat
     8941 ttatttcttt taaaataatt tcatagtcag gagttatttc aaaagatgtt tttaggtctt
     9001 ttattaaatt atttattcct tcacccattc ctgcaataga aatatcctga agttttcctg
     9061 ctttatttat aatttttaat atgattgtaa atctttccaa ttcatcaaca atatcttttt
     9121 cagaattttc ttctactaat tgacaggcaa gtgtttttat tgttaaaatt tctggcagta
     9181 agtttttttg gagaaaatta gaatataatt ttttaacttc ttttatttta gaaaaattag
     9241 aataaattaa aattgttttt tcttcttcct taataaaatt ttttaaaata tactctaatt
     9301 tacctctata agaaaatggg aatataatta actttttcat tcttttatat tataattatt
     9361 ttactatttc tttaaataca aacaaaatga gaaaaaaaga aaaaatcatt gggaaagtat
     9421 gttttagatt tattgtttat tatctttttt tacaggtcta ttatattaca tactttttac
     9481 ctactatata ttataaatat tgggctattt tatggggtaa ttttgcatat ctaactatgt
     9541 caaacagcaa aaaaagaatt ataataaacc tttcaattgc atttaaagat aaactttcat
     9601 acaaggataa gaaaaaaatt acaaaagaat ttttttatca gattgtttta aattttattg
     9661 aactggtaaa aatcacaaaa attcctatgt ctgaatatga aaaaatgata gaaattgaag
     9721 gagaagaaat tttgaaaaaa gttgtcgcag aaaaaaaagg tgtagtcgca gtatgctgtc
     9781 acctcggaaa ttttccagta gttcaggcat atttagcaaa aaagaattat ccaatcagta
     9841 tgattataag atattcaaat aatatttttt tatcaagatt taatgaccgt cttttaaaaa
     9901 aattgaaagt cccctttgta tcaaaatggg acttgaaaga agcaattgaa tactcaaaaa
     9961 aataccttaa aaaaggggga attgtatgtt tctatcttga ccagcacgat ggaaagggag
    10021 taaaagttga tttttttgga gaaaaagttt ttactccaac aggagcagcg gtctttgcaa
    10081 gaaaatataa atgtaatgtt atcggaattt tcacatatag aactaaaaaa aataaacata
    10141 aagtaataat tgaaggacct tacaaaaccg aatatacagc caatacaaga agtgatattg
    10201 aaaatatgag tgcatttttc ataaaaagag ttgaatatta tgtggaaaaa cacccagaac
    10261 agtggttcac atggttacat agaagattcc gttaaaaaat ttcctttaat atttttaatt
    10321 attttttctc ttatttcaga aataggtgtt ttttcttgtc ttaaaacttt ttcaatatag
    10381 ggaagatatt tttcagcggt cttttctcct tcttttacac aatattgaaa ttgagaaaaa
    10441 ttataccatt caataccttc aagttctatt tcaattttaa aatcagcata aatttcttct
    10501 aatatatcag catatttact tttaatttta cttacctgaa ctaatgtgct aaaaacattt
    10561 gaaggtctgt tctttaaaag tgatggttca ttttcaacac atattataat ttcaccacca
    10621 agaatatagt ttgatataac tggaatatta cttgcaactc caccatcaat gaattttttg
    10681 ccatttattt caacaagtgt gagcagccca ggcattgcac aagaagcaac aacagcagga
    10741 aataatttac ctctgttaaa aataaaaatt ttacctgtaa aatactcaac tgcgactata
    10801 cctaaattta tttttgaatt ttcaatatat atttcctcat taaaaatttt tcttacttcc
    10861 tcaattactt tttctgaatc aaaaagtcct gttttaaaac tatcactaaa aagaatatat
    10921 aaatttttaa atccatctgt tattttttct gttagtcctc ttttcctatg tctgataaaa
    10981 atttcttcta tttttttaat atttctatca tttataaacc ttttaaaaaa ctcttctaat
    11041 tgctttgagt cctttgtaaa agaatataaa cttccaacca ctgaacccat acttacacct
    11101 gtaattatat caaattcaat tccttctctt tcaaaaacct taattacgcc aatttgagat
    11161 aaacttaatg ctccaccacc accgagtgca agacctattt tcataaaacc accttataaa
    11221 caataaacat aaatataggt gttaaaagac caactgaaag attatcatca acaggtattg
    11281 gtaaaatctc gagaatagcc gataaaaaaa caccagtaaa aaaaactggg taagggattt
    11341 ctacaaaaaa caaaagtaaa aaaccaattg ctaaattaaa taaaattcct ccaattaaac
    11401 cttctaatgt tttgcctttg aaaatttctc tttttccata tttttttcca ataattccac
    11461 ttcctgcatc ttcaaaagta aggaaaaaaa gagaataaat tgcaatatcg tatgggaaaa
    11521 aaagtaaaac aaggaaattt gaaatcatga aataagtatc tcctgtaaat ctacctactt
    11581 tctttttaaa aacccctcca tatttttctg tcatccattt ccatacagaa ggatttttcc
    11641 acctttcaaa ttcaagagca ccaagcaaag taagaaaaaa tgttgaaatt gccaatggta
    11701 aaaaaatatc ctttgtaaga atataaaaaa aaggagcaat tgaacccatt acaattctat
    11761 aaatttttct tttccacatt accaattttt ttgcctctta tttcttttat tatgtttttg
    11821 tttataattg cacctgttaa aattagaatc aaagtagaaa taaaataata tataaattgc
    11881 tctttataaa taaagaaaac aaacaacaaa aatataaaga tacaacttaa aaggattttg
    11941 ggagaacgag gatttatttt ctgagcagta aaccataaga taataacaag gggaaaaagg
    12001 gatggcatta gagagagaat tacccctcca cttgttgctg ctcctttacc tcctttaaat
    12061 tttaaaaaga cagaataaca atgtccaata atagcaaaac atcctataat tgttagaaaa
    12121 attatatttg tttctcccca gcactttcca agaaaaatag gaaaagcacc tttaatagta
    12181 tcacaaaacc aaaccattat tccccatttt ttactgacat ttcttacaac attagcagcc
    12241 cctggattac catctcctat ttttcttata tctttaccaa ttagaaaata tgtaaaaaga
    12301 aaagcgaaag gaatacttcc acataaataa cttataaaaa acaagaaaac aattctcata
    12361 caataatttt aaaacaaaaa aatatatttt caaaaagtct aatttagata tactaaatct
    12421 ggatatactc actcgcacac gcaactaaaa atttacaggc acagcaggaa gagtgcataa
    12481 agttaaaggt aaaagcaaaa attaaaaatt tctattcttt tcttattttt caattggata
    12541 tttcccctca cgctttactt tttactctct taaaactttg tgtctaactt aacaatttac
    12601 accgagatct aagatattat aaaacaggaa ttaaagagtg aaatttcatg acttttcgtt
    12661 tggaaattta tcaataagtg atttggtgtt ttctcttaaa attttcttct ttacttcatc
    12721 aggtatatca gaaagtaaaa tag
//
LOCUS       DAOOVW010000044        12202 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_3401_length_12202_cov_8.839796, whole genome shotgun sequence.
ACCESSION   DAOOVW010000044 DAOOVW010000000
VERSION     DAOOVW010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12202
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3401_length_12202_cov_8.839796"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2822)
                     /locus_tag="PLW95_06395"
     CDS             complement(<1..2822)
                     /locus_tag="PLW95_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22292.1"
                     /translation="MKKIFIAILVFFSFIFKCNAATKISVSYKGLDSVHLQYEVEIGY
                     KADEIKIVCSDGEEAVIGSLPEGGGKLEGTKLHSELTEGNYTFQLVIDGVNYGLPVSV
                     NINGKIGGTLLYNETINDVATLEGNVVVPDGKTLTIKNKLIGSASYNSIYVDGTINFQ
                     SGAVLNGVNIHFRGEQSVNIDSIGGNGELIFYQKSTGSNIGNCKSKFLITVQPNTLVS
                     INKSEIYGLQLSEESSLSIKDSTVLSEFSISKIKSFEAENTTFLSPISINGRSPKFEN
                     CEFADFVTLHNRNEAEFKDCVFGSDLVFDDFSVYDLPKWSEDSSVHPTFSGNSFVGQS
                     GPRFEGLNGPFTPIYLGDDNYYGDKAPVIGNMLRTFLTRGTEINTLHFLVSNWNSSGS
                     EMKNKKKLPSFWVNDYVVGQNCLPHPSPWSGRKDHTLLKGRETLLSLDIATNYANVSG
                     VKFKAYLDGDTKGIDPVNTNVILHRDISNLGKDIFYGNTTVDFIIPATDKDSIYIEVK
                     IDTTGIEGFDDAPDKGVKQIFRKTLTFSSPYSRQLNIVVQPVQLFIAGYTKSAPDGKG
                     VENALKKLIPAMLPISKENINIWTAPVTTFHGGILSMLSSVALLNRIANTLAVAQGFI
                     NTTAAVGDWIAGEETPRIDFVVAVLPKGAMGEGITGASLKLRRGVIFVDETAPDAALH
                     ELGHGIGLYTGEEQYDEFPPAGLQVEGLTAFINDESAANSINGFRNRFLHFPRKGQSW
                     YEDKYWYDIMGSSTTLIWPIESTFSEFENYFQSTLGTKTKSASYKKAEVPTGYRRIFI
                     SAQTETPPGSPGYYQLIPETISVFDITEIATGKIDVSLEIAPYYNDDYKLECFNSSGK
                     NIYSQEFTTVSPYKGCRTSDWPKESTFCGTFDIPEDTQSIQISDTWGDIYYEFNRTDS
                     LPITITSVPSGTLSDSVDIAWTTTKQA"
     gene            complement(2939..3133)
                     /locus_tag="PLW95_06400"
     CDS             complement(2939..3133)
                     /locus_tag="PLW95_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22293.1"
                     /translation="MKEEIQRNPFPPFLYKRKEFIVVSPFVKGDCCPSFRKHLSDKIC
                     GGEEKGRFSKHNGASRSITA"
     gene            complement(3867..5705)
                     /locus_tag="PLW95_06405"
     CDS             complement(3867..5705)
                     /locus_tag="PLW95_06405"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HOV22294.1"
                     /translation="MRKFIFILFLFVFFSFFSEATISKWTIMIYLDGDNNLEEYALDD
                     FLEMSEISSDENVNIVVQFDRISRYDSSYGNWTGCNRFYVTKGIEPTEENAISNWGDG
                     KGEREVNMGDPETLANFILWAKENYPAEKYTLILWNHGGGWRDLYQSMYQIAGKEIPV
                     LKEVCYDDTDGDYLSTNEVRQAIENAGGVDLIGFDACLMGMIEVAYELSPFASVMVAS
                     EEVEPASGWDYVEILSELKAQPLSTSEEFGEIIVNSYSYDTLSAISLINIQQLIDSLN
                     YVLDEIMSSDIYLDIYLVRSETKNFDELTYIDLYDFFNKLYQTTENETLKSKIEEFQD
                     IFKQTVFSDNEESDNSYGLSIYFPDYGEVIAFDYNGETISFPESALWDEFLSAFLTSD
                     LFSSFTLVFSEDFSSGIPVNWTIVDGYNDGKTWTYTNPGRRNLDLNSPFMIVDSDWAG
                     TVDMDEELITPSIDFTSYATCYLEFKNVFEYYENEIGDVDIKIGNGQWQNLKKFKRQD
                     IEGKVIIDLTSISSGEKNVQIRWHYYNANWDWYWAVDDIKIYGKLPGKGDVDKSGGID
                     ISDVILCLRMAIELDPLDTSLADINGDGEVDITDVILILRRVVGLT"
     gene            complement(5702..7240)
                     /locus_tag="PLW95_06410"
     CDS             complement(5702..7240)
                     /locus_tag="PLW95_06410"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HOV22295.1"
                     /translation="MCKKVIFLVFFLLIGSVFSATITIENLSVSSSVSEITIPIKISS
                     EEKFSAFQFDVYFDKEILNFDGISNGELVFDWNVLVNEVSEGNIRIAGFDPKLEGISG
                     NGVLVYVRFLIKKGGKVNMSIRGIRLSDKDGKKLNCIGITSTIQIEGEKGETKENEDS
                     QSSLPETPELSSPTSTIKPIIRNTPGERITNQGRESNIPVRSERETSKSKTYQVEQQG
                     NFAPTSNNCILLVMSEYGNPSPPNGFTTFTKGESVICKVEDRVPISEMEKVVCTGYEG
                     TGAARNGNSNQANFIITEDSKILWKWKKVPMEREFNLKYEENVYIPYDKNKIEIPLSC
                     DYYGGLNSKILFGKEIIPDGIEIEFPVINYEKNKGEIVIKVDKNNVKGGNYKIIFSAF
                     PETEETMKKEFQINLSVETILEIEKQKNDDKTSFLFKSDKLGDFKNFFIEIEKKGKIK
                     IEDIKPEKTDIIIRKNSFSIAGNKEIFDKLVITLSGDVENLKIEKAKFIDKDGEYIKV
                     KIPL"
     gene            complement(7392..7550)
                     /locus_tag="PLW95_06415"
     CDS             complement(7392..7550)
                     /locus_tag="PLW95_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22296.1"
                     /translation="MIKEQTGLSEYKILKCCNKIREIMQRDIPKNFGWIVEVNETYFG
                     RQWKNKNK"
     gene            7878..8690
                     /gene="amrB"
                     /locus_tag="PLW95_06420"
     CDS             7878..8690
                     /gene="amrB"
                     /locus_tag="PLW95_06420"
                     /inference="COORDINATES: protein motif:HMM:NF001987.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOV22297.1"
                     /translation="MENVREPVVAGYFYPSSKSQLLRMLEEFIKKDDEKEDAISIVVP
                     HAGYIYSGKTAGSVYSKIVVPETVIIIGPNHTGYGEPYAVASYDWWLTPIGKIPVNKN
                     LVNLLVEKSRYLQIDNFAHEREHSVEVQLPFLQVVKSNFKIVPITLMGYIDNPAWIEI
                     GEAIAESIKELGEKVLIVASSDMTHYKPHDIAEENDKYAIEAICCLDEDLLIERILER
                     EISICGYAPIIVSIVASKILGAQQGDLIRYTTSGETSGDRDQVVGYAGILIK"
     gene            8715..10625
                     /locus_tag="PLW95_06425"
     CDS             8715..10625
                     /locus_tag="PLW95_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22298.1"
                     /translation="MFIFLLISLSVASIFYSLKLCEKDNFPEFLLIFLIIFYSQILCF
                     SIALGLFHILNLKFLFFFSLVSVIISILKYKKETIKIFLTSSFSNFNIIIFSLVFSFY
                     FFACIQLSFLLPPLATDGLLYHLPFAVHFLKTSSISLCPLYFVDIAMSYYPFGGEIFY
                     FFTLLSQKDFLLKFTQLPFLIMGTISVFLISKELSFSNISSLLTSLIFAMIKPIFQEA
                     SLCFVDLMMASTFLASIYFLLKDKKKYIVLAILSCTILISIKTLSLIYFLFLVPFFFK
                     KRDGKYIKKDIFFSLVYFFFFGFFSYIRNFIVTKNPFYPANISIGKFTIFPGSYIYKK
                     GLPINCFKILFHPQSHIDPSFFIISIMFLFLFISIFIYCIEKRKFPYLFFLPFFIILG
                     YFILIPPNYYQIRHLLPIYGICAIAFVFPFQKGYFQYLPFLFLFYFFCLLFPYNHQNV
                     YLFLIIFTIFTISFYLIKKFPIFNFIFFFFFFLYLSVYLFPVATFSYNKVKFKIWGTF
                     YKDFADVWEFVGKSKNKNISYVGGFLLYPFFGENYSNNLYYQSVNSVETYPVHFYKGK
                     IVFSEENPEKIYRKDPSFNLWFMGLKKKKIDWVVLKKDEYYIEKEWIEKNPQYFKLIF
                     SGKITEIYNFLY"
     gene            complement(10661..11743)
                     /locus_tag="PLW95_06430"
     CDS             complement(10661..11743)
                     /locus_tag="PLW95_06430"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOV22299.1"
                     /translation="MYLILNIIAFLVSMSTSIVDVSIPMYLTEKYNVSKFSIGLSGFL
                     INFSYTITTFIFSKYEEEKIFPFTIFFIGLFYLLLSALPWKVFLISLLLIGFLFGRFW
                     PNIQLSFRKTEPKHTGYFNISWSFGVIIGFFLSGFIYSIRNSLPFIISGLLLIILGIY
                     FILFKKNHLSPIINKNNFEGIERKNINKKDIKDVRILNFLSYFAVGYSFFLFPRYGLE
                     IKLSPKNISFLVSSLQVFNFLTFFLIRNKPVFKNKFFFPFACFFFSLSFFLFLFLKKP
                     VEFLIPFSIIGILSAISYHNSLISHLKLGFKTEIHEFFIGLGSSMGPICCGFLSNMFE
                     FKNTFLIVSSIYAFLGICKLLFLKSN"
BASE COUNT         3905 a         2164 c         1623 g         4510 t
ORIGIN      
        1 gcctgttttg ttgttgtcca tgcaatatct acgctatctg ataatgtgcc ggagggaaca
       61 gatgtaattg ttataggaag agaatcagtg cggttaaatt cataataaat atcaccccag
      121 gtatcactaa tctggataga ttgtgtatct tctggaatat caaatgtccc gcagaaagta
      181 ctctctttag gccagtcgga agtcctacat cctttatatg gagaaactgt tgtgaattcc
      241 tgcgagtata tattttttcc agaactatta aaacattcta atttgtaatc atcattataa
      301 tatggggcta tttccaaaga tacatctatt ttacctgttg caatttctgt tatatcaaaa
      361 acagaaattg tttctggaat taattgataa tatccaggac ttcctggagg tgtttctgtt
      421 tgtgcggaaa taaaaattct tctataacct gttggaactt ctgctttttt gtaacttgca
      481 gattttgttt ttgtgccgag ggttgattga aaataattct caaattcaga aaaagttgat
      541 tcaattggcc atataagagt ggtagaagaa cccattatat cgtaccagta tttatcctca
      601 taccatgact gcccttttct tggaaaatgt agaaacctat ttctaaaacc attgattgaa
      661 ttggcagcag attcatcatt tataaaagca gtaagtcctt ctacttgcag tcctgctggt
      721 ggaaattcat catattgctc ttctcctgta taaagtccta ttccatgtcc caactcatgt
      781 aaagcggcat caggagcagt ttcatccaca aaaattacgc ctctccttaa cttcaaactt
      841 gctcctgtta taccttctcc catcgctcct tttggcaaaa ctgcaactac aaaatcaatc
      901 cgcggcgttt cttcccctgc tatccaatca cccacggcag cagttgtatt gataaatccc
      961 tgggcgactg cgagagtatt ggctattcgg ttcaaaaggg cgacactgct cagcattgat
     1021 aatattccac catgaaaagt cgttaccggc gcggtccaga tattaatgtt ttcttttgat
     1081 attggcagca tagccggtat gagtttcttc agggcattct ctacgccttt accatcaggc
     1141 gcacttttgg tatatccggc aataaataac tgaactggct gaacaactat atttaattgc
     1201 ctgctatatg gagaagaaaa tgttaaagtt ttacgaaaga tttgtttcac acccttatca
     1261 ggagcatcat caaacccctc aatacctgtt gtatctattt ttacttctat ataaattgaa
     1321 tccttatctg tcgccggaat gatgaaatca acggtggtat tgccataaaa aatatctttg
     1381 cctaaatttg aaatatctct atgcaggatt acatttgtat ttacagggtc tattcctttt
     1441 gtatctccat ccaaatatgc tttgaatttc acccctgaaa catttgcata gtttgttgct
     1501 atatcaaggg ataatagtgt ttctctacct ttaagaagag tatggtcttt tctacctgac
     1561 catggtgaag gatggggcag acaattctga ccgaccacat aatcatttac ccagaaagaa
     1621 ggcaactttt ttttgttttt catttcagac ccagaactat tccaattaga aacaagaaaa
     1681 tgcagagtat taatttccgt tcctctggtt aaaaatgtcc gtaacatatt accaatcacc
     1741 ggcgctttat caccataata attatcatct cccaagtaaa tcggagtaaa aggtccgttt
     1801 agtccttcaa acctggggcc gctctgccca acaaaactat taccggaaaa agtgggatga
     1861 actgaagagt cctctgacca ttttggcaaa tcatatacag agaaatcgtc aaaaacaagg
     1921 tctgaaccaa aaacacaatc tttaaattcg gcttcatttc tgttgtgtaa ggtaacaaaa
     1981 tcggcgaatt cgcaattttc aaattttgga ctccttccgt taattgaaat aggagataaa
     2041 aaggttgtgt tttccgcttc aaaactcttt atttttgaaa tagaaaactc tgataaaacc
     2101 gttgaatctt tgatggaaag agaggactcc tctgatagtt gaagtccata tatttctgac
     2161 ttgttaatag atacaagagt atttggctgg acagtaatta aaaatttgct tttacaattc
     2221 ccaatattag aaccagtgga tttttgataa aaaatcagtt ctccatttcc tcctatgcta
     2281 tcaatattta ctgactgctc tccccgaaaa tggatattca caccattcaa taccgcacca
     2341 ctttgaaaat ttattgttcc atctacataa atagaattat atgaagcgct tccgataagt
     2401 ttgttcttta tagtcaaggt tttaccatcc gggacaacaa cattgccttc cagagttgcc
     2461 acatcgttta ttgtttcatt atacaggaga gttcccccga tttttccgtt tatattcact
     2521 gatacaggca aaccataatt aacaccatca attactaatt ggaaagtata atttccttct
     2581 gtaagttcac tatgaagttt tgtcccttca agttttcctc ctccctctgg taaacttcct
     2641 ataactgctt cttctccatc actacaaaca attttaattt catctgcttt gtatccaatt
     2701 tcaacttcgt attgaagatg aacactatca agacctttat aactaacact aatttttgta
     2761 gcagcattac atttgaaaat aaaagaaaaa aacaccaata ttgcaataaa aatttttttc
     2821 attttccttt tctccttttt ttgtattttt tagtattacc gcgggggtta aattcacgcg
     2881 gttatacttc tacttctggt ataacattgc agttctattc ggctactgat agttaaattc
     2941 acgcggttat acttctactt gctccgttgt gcttgctaaa tctccccttc tcctctccgc
     3001 cacaaatctt gtcggacaga tgctttctaa aagaggggca acaatctccc tttacaaaag
     3061 gggaaactac gataaattcc ttccttttat aaaggaaggg tgggaatgga tttctctgta
     3121 tctcctcttt caatccccct ctttccacct tttttctccg attgctccct cctttgacaa
     3181 aggagggcgg gggtggattt ctaaatcccc ctctatcccc ctttattaaa gggggaatat
     3241 tttgtatgac gcaccaactc tgccctgcta aaccctgctt ccttagtcgg tttttcaaag
     3301 ggggaactta tgggtaaggg acagccctct ttacaaaaga ggggagttac tccttccctt
     3361 tataaagcat gtccgccaat gttttagtgc gatagtccgc caaagtatca gtggcggaga
     3421 ggagaagggg agctcgcccg tagggctatg cccgagggaa tggattgtct tacacagaag
     3481 ggtttagagt tttgcttaca gaccatccct acgccgaagt gataagttat acttacggga
     3541 ttttcattta taaatccccc tcagcccccc tttctaaaag gggggaatat tccttccttt
     3601 aacaaaggaa ggtctctctt tttgccacaa tatctaaccc gcaggacaag gaatatccct
     3661 tcctttaaca aaggaaggtt aggatggatt tctaaatctc cccctacccc tctttctaaa
     3721 agaggggagt attttgggat gacgcaccaa ctctgaggca caaggtaagc aacttttgtc
     3781 ggtttcccaa aaaggggata ttttgggatg gagttttttt tattttctgc ctgtttttat
     3841 ttcctcataa agagtttgtc aaattatcag gtaagtccaa ctactcttct taatatcaaa
     3901 attacatctg ttatatccac ttctccatct ccatttatat ctgccagaga agtatctaga
     3961 gggtctaatt caattgccat cctcaaacat aatattacat ctgatatatc tattccccca
     4021 cttttatcaa catctccttt tcccggtaat ttcccatata tttttatatc gtcaactgcc
     4081 caataccaat cccaatttgc attataataa tgccatctta tttgaacatt tttttccccg
     4141 gaagatattg aagttaaatc aataattact tttccttcta tatcctgtct tttaaatttt
     4201 tttaaatttt gccattgccc atttcctatt tttatgtcaa catctcctat ttcattttca
     4261 taatattcaa aaacattttt aaattctaaa taacaggtag catatgaagt aaaatcaata
     4321 gaaggggtta taagttcctc atccatatca actgttcctg cccaatcact atcaacaatc
     4381 ataaaaggag agtttaaatc caaatttctt ctaccagggt ttgtatatgt ccatgttttt
     4441 ccatcattat atccatcaac aatagtccag ttaacaggaa tacctgatga aaagtcctct
     4501 gaaaaaacaa gtgtaaaaga agaaaataaa tcagaagtta agaaagcaga taaaaactca
     4561 tcccacaatg cactttctgg gaaagaaatt gtttccccat tataatcaaa agcaattact
     4621 tctccataat caggaaagta aattgaaagt ccataagaat tatcactttc ctcattatca
     4681 gaaaaaactg tttgcttaaa aatatcttga aattcctcaa tttttgactt taatgtttca
     4741 ttttctgttg tctgataaag tttattaaaa aaatcataaa ggtcaatata agttagttca
     4801 tcaaaatttt ttgtctcact tctaactaaa taaatatcta aatatatgtc actgctcata
     4861 atctcatcta acacataatt taaagaatct ataagttgtt gtatatttat aagagaaatt
     4921 gcggaaagtg tatcataaga atatgaattt acaattattt caccgaattc ttcagatgta
     4981 gataatggtt gtgctttcaa ttcacttaaa atttcaacat aatcccagcc agaggcaggt
     5041 tcaacttctt ctgatgcgac cataacagaa gcgaaaggac ttaactcata ggcaacttca
     5101 atcataccca tcaaacaggc atcaaatcca attaaatcca ctccaccagc attttcaatt
     5161 gcctgtctta cttcatttgt tgaaagatag tccccgtctg tatcatcata acaaacttct
     5221 tttaaaacag gtatttcttt acctgctatt tgatacattg actgatataa atctctccaa
     5281 ccacctccgt gattccataa aattaaagta tatttttctg ctgggtaatt ttcttttgcc
     5341 cagagaataa aatttgccag tgtttcaggg tctcccatat ttacttctct ctcccctttt
     5401 ccatctcccc agttagaaat tgcattttct tctgttggtt ctattccctt tgttacataa
     5461 aatctattac aaccagtcca attcccataa ctactgtcat aacgagaaat tctatcaaat
     5521 tgaacaacta tattaacatt ttcatcagaa ctaatttcac tcatttctaa aaagtcatcc
     5581 aaagcatatt cttcaaggtt attgtctcca tctaaataaa tcataattgt ccactttgaa
     5641 attgttgctt cagaaaagaa agagaaaaaa acaaaaagga aaaggataaa tataaatttt
     5701 ctcataaagg tatttttact tttatatatt ccccatcttt atcaataaat tttgcttttt
     5761 caatttttaa attctctaca tctcctgata atgtaataac aagtttatca aatatttctt
     5821 tattaccagc aattgaaaaa gagttttttc ttataataat atctgttttt tctggtttta
     5881 tatcttcaat ttttatttta cctttttttt caatttcaat gaagaaattt ttaaaatcac
     5941 ctaatttatc tgacttaaat aaaaaagatg ttttatcatc atttttttgt ttttcaattt
     6001 ccagaattgt ttcaacagaa agatttattt gaaattcttt tttcattgtt tcttcagttt
     6061 caggaaaggc agaaaaaata attttataat tccctccttt tacattattt ttgtccactt
     6121 tgattactat ctctccttta ttcttttcat aatttatcac aggaaattca atttcaatac
     6181 catctggaat tatttctttc ccaaataata tttttgaatt aagtccacca tagtaatcac
     6241 aacttaaagg tatttcaatt ttatttttat cataaggaat atacacattt tcttcatatt
     6301 ttaaattaaa ctccctttcc attgggactt ttttccattt ccataaaatt ttactgtcct
     6361 ctgttattat aaaatttgcc tgatttgaat ttccatttct ggcagcacca gttccttcat
     6421 aaccagtgca tacaactttt tccatttcac ttattggcac tctatcctct accttacaaa
     6481 taacactttc tccttttgta aatgtcgtaa aaccatttgg aggagatgga tttccatatt
     6541 cgctcataac aagaagaatg caattatttg aagttggagc aaaatttccc tgttgttcta
     6601 cctgatatgt tttacttttt gaagtttctc tctctgaccg aacaggaata ttactttctc
     6661 tgccctggtt tgttattctt tctcctggtg tatttcttat tattggttta atagttgatg
     6721 ttggagaaga aagttctggt gtttcaggta aagaactttg agaatcttca ttttcttttg
     6781 tttccccctt ttcaccttca atctggatag tagatgtaat ccctatacaa tttagttttt
     6841 tcccatcttt atcagataat cttatccctc ttatagacat atttacctta ccaccttttt
     6901 taattaaaaa tctgacatat actaacacac catttcctga aataccttca agttttgggt
     6961 caaaaccagc aattcttata tttccttctg atacttcatt taccagaaca ttccaatcaa
     7021 aaacaagttc tccatttgaa attccatcaa aattaagaat ctctttatca aaataaacat
     7081 caaattggaa agcagaaaac ttttcttcac ttgatatttt tataggaata gttatttcac
     7141 ttacagaaga agaaacagat aaattctcaa ttgtaattgt agcagaaaaa acagaaccta
     7201 taagtaagaa aaataccaaa aaaattactt ttttacacat tttctttgtg gaagtaaata
     7261 cttattaatc tctattcatg tttcaggtcc agaagggtta ttttgatttt agccactaaa
     7321 tttaattact aaaattattt taaaacatat tttttaaatg tcaaattttt ttcaaaagca
     7381 aaaaaagcaa cttatttatt tttattcttc cattgtcttc caaaatatgt ctcatttact
     7441 tctactatcc atccaaaatt ctttggaata tctctttgca taatttctct tattttatta
     7501 caacatttta aaattttata ctctgataat cccgtttgct cttttatcat ttttaccctt
     7561 ttagatatta aaaatcacct caaaaatttc ttccattcat atctgcaatt tttacacctt
     7621 attttacccc ttctctttct tcctcgctct ctgatacctc tctgctattt tttacttatt
     7681 ttcttcctta ttcccatttt taattccttt ttcatttgca cctccggttt tttcttattt
     7741 tatccgaagg tgctatttaa atcctatttt tcgagtagat tcctattttg tcttgtctaa
     7801 catacctttt ggagtagatt tattatgtct caattcgttg gtttgaagtt tatcatttta
     7861 aaaggtataa tatttttatg gaaaatgtaa gagaaccagt agtagcaggt tatttttatc
     7921 catcttcaaa gtcacaactt ttaagaatgc ttgaagagtt tatcaaaaaa gatgatgaaa
     7981 aagaagatgc aatatcaata gtggttcctc atgcgggcta tatttattct gggaaaacag
     8041 cagggagtgt ctattcaaaa attgtagttc ctgaaactgt tataattatc ggtccaaatc
     8101 atactggata tggagaacca tatgcagttg cttcttatga ttggtggctt actcctattg
     8161 gtaaaattcc tgttaataag aaccttgtta atcttcttgt tgagaaaagt agatatcttc
     8221 aaatagataa ttttgctcat gaaagagaac attctgttga agtacaactg ccttttcttc
     8281 aagttgtaaa aagtaatttt aagattgttc caataacttt gatgggatat attgataatc
     8341 cggcgtggat tgaaatagga gaggcaattg ctgaaagtat aaaagaactt ggagaaaagg
     8401 ttctaattgt tgccagttct gatatgaccc attataaacc acatgatatt gctgaagaaa
     8461 atgataagta tgctattgaa gcaatttgtt gtttagatga agacctttta attgaaagga
     8521 tacttgaaag ggaaatttca atatgtgggt atgctccaat aattgtctca attgttgctt
     8581 caaaaatttt aggagcgcaa caaggagacc tcataagata tacaacaagt ggagaaactt
     8641 caggtgacag ggaccaggta gttggttatg caggaatact tataaaatag gaattatgac
     8701 aagggagttt gagtttgttt atatttcttt taatttctct ttcagttgca agtatttttt
     8761 attctctaaa actttgtgaa aaagataatt ttccagaatt tttgctcatt tttttaatta
     8821 tcttttattc tcaaatactt tgtttttcta tagctttagg tctttttcat attttaaatt
     8881 taaaatttct tttctttttt tctcttgttt ctgttattat ttctatttta aaatataaaa
     8941 aagaaactat taaaatcttt ttaacatcat ctttttctaa ctttaatatt attatttttt
     9001 cccttgtttt ctctttttat ttctttgcct gtattcaact ttcttttcta ctacctcctc
     9061 ttgcaacaga tggacttctt taccatcttc cttttgcagt tcacttttta aaaacatcct
     9121 caatttcttt atgccctctt tattttgttg atattgctat gagttattat ccctttgggg
     9181 gagaaatatt ttattttttt actttacttt cacaaaagga ctttttactt aaattcacgc
     9241 aattgccttt tcttattatg ggaactattt ctgttttttt aatctccaag gaactttctt
     9301 tctcaaacat ttcatcactc ctaacttctt taatttttgc tatgataaaa cctatttttc
     9361 aagaagcatc tctatgcttt gttgatctta tgatggcttc aacttttctt gcttctatct
     9421 attttttatt aaaagacaag aaaaaatata ttgttttagc aattctttca tgtacaattt
     9481 taatttcaat aaaaacactt tctttaattt attttctttt tttagttcct tttttcttta
     9541 agaaaagaga tgggaaatac ataaaaaaag atatattttt ctcattagtt tatttctttt
     9601 tctttggatt tttttcatat ataagaaatt tcattgttac aaaaaatcca ttttacccgg
     9661 ctaatatctc tattggaaaa tttacaattt ttcccggctc atacatttac aaaaagggac
     9721 ttccaataaa ttgctttaaa attttatttc acccacaatc tcatatagac ccttcttttt
     9781 ttataatttc tattatgttt ctatttctat ttatttctat ttttatttat tgtatagaaa
     9841 aaagaaaatt cccttattta ttttttcttc ctttttttat aattttggga tattttattt
     9901 taattcctcc taattattat caaataagac atctccttcc aatttatgga atttgtgcaa
     9961 ttgcctttgt ttttccattt caaaaaggat actttcaata tcttcctttt ttatttcttt
    10021 tttatttctt ttgtctcctt tttccttata accaccaaaa tgtttatctt ttccttatta
    10081 tctttacaat ttttacaatt tctttttatt tgattaaaaa atttcctatt tttaatttta
    10141 ttttcttctt tttctttttt ctctatcttt ccgtttattt atttcctgtt gcaacttttt
    10201 catataataa agtcaagttt aaaatatggg gaacatttta taaggacttt gctgatgtgt
    10261 gggaatttgt tggtaaaagc aaaaacaaaa atatctctta tgttggtggg tttcttttat
    10321 atccattttt tggagaaaat tattccaata atctttatta tcaatctgta aattctgttg
    10381 aaacatatcc agtccatttt tacaaaggaa aaattgtttt ctctgaagaa aatccagaaa
    10441 aaatttatag aaaggaccca tcatttaatc tctggtttat gggactaaaa aagaaaaaaa
    10501 ttgattgggt agttttgaaa aaagatgaat attatattga aaaagaatgg attgaaaaaa
    10561 accctcaata ttttaaatta atattttcag gtaaaattac agaaatttat aattttttat
    10621 actgatagtt ttggatattt tatttataat tatatgtttt ttaattactt tttaagaata
    10681 ataatttaca aataccaaga aaagcataaa tagaagatac tatcaaaaat gtatttttaa
    10741 attcaaacat attacttaaa aaaccacagc aaataggacc catagaacta cctaatccta
    10801 taaaaaattc atgtatctct gttttaaatc caagttttag gtgtgaaatt aaactattat
    10861 gataagaaat agcacttaat atacctatta ttgaaaaagg aattaaaaat tcaactggtt
    10921 ttttcaaaaa aagaaacaga aaaaatgaca gagaaaagaa aaaacaggca aaagggaaaa
    10981 agaatttatt tttaaaaaca ggtttatttc ttattagaaa gaatgttaaa aaattaaaaa
    11041 cctgaagaga agaaacaaga aaacttatat ttttaggtga taattttatt tcaagtccat
    11101 atcttggaaa aagaaaaaat gaataaccaa ctgcaaaata acttaaaaaa ttcaaaattc
    11161 ttacatcttt tatatctttt ttatttatat tttttctctc aattccttca aagttattct
    11221 tatttataat aggagataga tgattttttt tgaataaaat aaagtaaata ccaagaatta
    11281 taagaagcaa acccgatata ataaatggta aagaatttct aattgaataa ataaaacctg
    11341 aaaggaaaaa tccaataata acaccaaatg accatgaaat attaaaatat cctgtatgtt
    11401 ttggttctgt ttttctgaaa gataattgaa tatttggcca gaatcttcca aatagaaacc
    11461 caattagtaa tagtgatatt aaaaaaactt tccatggcaa agcagataat aaaagataaa
    11521 ataaaccaat aaagaaaatt gtaaagggga aaattttttc ttcttcatat tttgaaaaaa
    11581 taaatgttgt aattgtatat gaaaaattta ttaaaaagcc agaaagtcca atactgaatt
    11641 tactcacatt gtatttttca gtgagataca ttggaataga cacatctact atactggttg
    11701 acatactaac taaaaaagca attatattta aaattaagta catttttata ataatttggt
    11761 aattttaaaa tttttgtctt aatttttact taattaaacc aatatatcat accataggta
    11821 aaccctaaaa caattaggaa atgataaatt tctttaaaaa atcttataca aaagcaatta
    11881 atatggtata ataaaaagta ataagtcagt agaaccttta tgttaggcac aaatgaaaat
    11941 ccatcccctc gggcatagcc ctacgggcga gcccaccttc tcctccacaa agcgtggcgg
    12001 acaggatttg ttaaaggaag gaataagaac aaaaaaaatc ccctctttga aaaaccgaca
    12061 aaagcagatt accttgtgcc tcagagttgg tgcgtcatac aaaatattcc ccctttaaca
    12121 aagggggatg gagggggatt taatatgaaa tccatcccat ccttcctttt tcaaaggaag
    12181 gagtatattt ttccccttta at
//
LOCUS       DAOOVW010000045        11838 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_3540_length_11838_cov_7.502673, whole genome shotgun sequence.
ACCESSION   DAOOVW010000045 DAOOVW010000000
VERSION     DAOOVW010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11838
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3540_length_11838_cov_7.502673"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(457..2022)
                     /locus_tag="PLW95_06435"
     rRNA            complement(457..2022)
                     /locus_tag="PLW95_06435"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(2103..2318)
                     /locus_tag="PLW95_06440"
     CDS             complement(2103..2318)
                     /locus_tag="PLW95_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22300.1"
                     /translation="MAEKKMNPAALVILIIIILVALFFIIKQMTKKPAATPEAMPPAM
                     EGPMGPEGMPAPEAPAPEAPAPEAPAE"
     gene            complement(2379..2963)
                     /locus_tag="PLW95_06445"
     CDS             complement(2379..2963)
                     /locus_tag="PLW95_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOV22301.1"
                     /translation="MDIYEVIKKRRTIRRFEQKPISNELLIKFIDMARLAPSASNLQP
                     LEYIVVNKKEVVDEIFPNLAWAGYLGEKGQPPEGKRPVSYIVVIANKQINVLKYKLEC
                     GAAIENILLCATYEGIGTCWIGSVNRKQVRKILQIPGNYIIDSVIALGYPAEKSVIEE
                     FNGSVKYWKDEQEIMHVPKRNLKDIIHLNKFEVK"
     assembly_gap    3157..3256
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3393..3468)
                     /locus_tag="PLW95_06450"
     tRNA            complement(3393..3468)
                     /locus_tag="PLW95_06450"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3430..3432),aa:Pro,seq:ggg)
     gene            complement(3470..4273)
                     /locus_tag="PLW95_06455"
     CDS             complement(3470..4273)
                     /locus_tag="PLW95_06455"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF005719.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOV22302.1"
                     /translation="MIIIGERINGTREHIKKAIEEKDKNYIKREIRKQIENGATYIDL
                     NAGTGKGQEREKQDIKWLIEITKEENISKICIDSSDPDVIKFALAFVENNDIIINSIN
                     GEKDKKKKLLDIIKRFPECKIIVLTMDDNGIPKTIEKKIEIAKNLIDALSQIGMKKEN
                     IFIDCLVEPISVDIKNAMNFLKCVKKIKEEVPEVKTTCGLSNVSFGLPKRKIVNKYFL
                     SLCIYEGIDSVIIDPTIVDIKEGLYTTNLLTGNDEFCMNYIRGIREGRF"
     gene            complement(4293..>4694)
                     /locus_tag="PLW95_06460"
     CDS             complement(4293..>4694)
                     /locus_tag="PLW95_06460"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOV22303.1"
                     /translation="LYPETMIGTGEEIIRIIEEFNSPYLGVCFDTGHAHTIGKFKEEF
                     IALKDKIFTFHLHDNDGERDLHLPPIQGTIDWKWFIKELKKSNYSGQLPIEIELPKYL
                     KWSEVVSNMKKLVENDGEGEFLYGNRIIKII"
     assembly_gap    4695..4794
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5032..5370
                     /locus_tag="PLW95_06465"
     CDS             5032..5370
                     /locus_tag="PLW95_06465"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HOV22304.1"
                     /translation="MEISKEVFEEIIYQKLNCLPKEIKDKLENICFIVEENAPSYTLL
                     GLYHGVPYPKRGPSYSLVLPDRIILYKRNIEAGCKTEKELIKRIEKVLFHEIGHYLGL
                     EEKQLRKLGL"
     gene            complement(5365..5931)
                     /gene="pyrE"
                     /locus_tag="PLW95_06470"
     CDS             complement(5365..5931)
                     /gene="pyrE"
                     /locus_tag="PLW95_06470"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOV22305.1"
                     /translation="MNDILKILKETGAILTGHFLLSSGLHSDTYFQMAIIFQYPDYGQ
                     ILCENLANNFKEKKINVVIGPAIGGIIISYELARILKARSIFAEREEGKMKLRRGFKI
                     EKGEKVLICEDVITTGGSVREIIDIVEKVKGQIEGIGCIVERGKIEFNYPLRSLVKLE
                     VKNYNSSECPLCKRGIPLVKPGSKNIDL"
     gene            complement(5976..6866)
                     /locus_tag="PLW95_06475"
     CDS             complement(5976..6866)
                     /locus_tag="PLW95_06475"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOV22306.1"
                     /translation="MPEIYLVNGKVKQKKDIGLPILNRGFLFGDGIFETIISKNYSLF
                     RFFEHYERLKKGADFCGFRIPPKNEIEKSILKYLKKFRIKNGYIRINVWRKKGKSVIP
                     EGKETNILIILKNFTSYPKYFYERGISCIISEKIIRNEKSPLTKIKSFNFLENIIGKI
                     EAKENGCEDTIFLNNNDFLTETTVSNLFLVKNNIVYTPSLDCGILNGITRKIIIEICQ
                     KNKIKIKEGKFTAKTLSECDEIFITNTLMGIMPVGKVKNFFQNNRNEMTDFIIKKYIE
                     ILKEETIFFKKNKVSQKSMD"
     gene            complement(6844..8235)
                     /gene="pabB"
                     /locus_tag="PLW95_06480"
     CDS             complement(6844..8235)
                     /gene="pabB"
                     /locus_tag="PLW95_06480"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HOV22307.1"
                     /translation="MMNIFFEYLNYLPPELIFKSFHTKKFFFFLDSVSPSYGRYSIVG
                     FEPFLIFKSKGDKITIEDTNSKKTYIGKPFDYLNELLEKYWVDTRFFFIPGAVGYLGY
                     DAGLKFENLPDINPDILKIPDIFFCFYDTFLIFDIIKKKLILTGINFFEKESTFKKKI
                     SLISSEIKNSLKFQNDEKEAKIKNFSSNMKFSEYVSSIEKIKNYIKQGDVYQINFSQR
                     FQAEGIFNPSEIYLKIRKINPSGYSAYFDFNNFQILSNSPELFIKKYGIKIITKPMKG
                     TISRGKNRKEDSENKKELLDSEKDRAELIMIVDLERNDIGKICEFGSIKVKKLVEIEK
                     YKTCFQTTSTIEGILKKEVAPVDIIKNIFPGGSITGAPKIRAMEIIEELEPEKRSFYT
                     GSLGYIGFNKNMLLNILIRTILHKENKIYYPVGGGIVWDSIPEKEYEETLIKTKNLFL
                     TLGIDQNAGNLFS"
     gene            complement(8232..8669)
                     /gene="rpiB"
                     /locus_tag="PLW95_06485"
     CDS             complement(8232..8669)
                     /gene="rpiB"
                     /locus_tag="PLW95_06485"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012057395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOV22308.1"
                     /translation="MKIGIASDHAGFEMKDFLKKELENDGYEIIDYGPEIKKVVDYPD
                     FAEKVASGIIRKEIEFGILICGTGIGMSISANKFKGIRAARCCTIYDALLARQHNDAN
                     ILTLGGRLLGNTLSLEISKTFFETPFLKGRHLKRVNKIKKLEK"
     gene            complement(8666..9640)
                     /locus_tag="PLW95_06490"
     CDS             complement(8666..9640)
                     /locus_tag="PLW95_06490"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOV22309.1"
                     /translation="MEKILEEKEENIKVVVNTLKKGGVVIVPTDTVYGLISGGFNDRS
                     KEKIYEIKKRDYKKMLIGFVDTIEKAEKYIEIDRQFIKEKWPGSTTIIGKSKIEIPFL
                     TSDEKKIGLRIPDNKFLLDVLKNFEIIASTSANISGEKTPSCLSEISDEVKNKVDLII
                     NGGKTEGKESTIWDVSFSPPKLVRGKVIFVCEGNSCRSPMAEYFLKNFLKEKNVKIEV
                     NSAGTGITSWGKISDKTLEVMKKVGIEIKNFVSKPLNFQMLKESDLIFVMEKEQKEKI
                     LSIIPKEENKIFVLDVPDPAGKELFHYEEIRDIIKEKVKNIVLKRMAI"
     gene            complement(9645..10496)
                     /locus_tag="PLW95_06495"
     CDS             complement(9645..10496)
                     /locus_tag="PLW95_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012308998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOV22310.1"
                     /translation="MNKIIKKEQLAEKIKRIEVISPIISKKAKPGQFIVLRVDEKGER
                     IPLTIADVNKEKETITLIFQEVGTTTAKLGFLENGENILDIVGPLGKATEIEKYGNVC
                     IIVGGVGAAFIFWMAKTFKEKENYLITIIGARNKDLIILEEELKNISDEIYITTDDGS
                     KGRKGFNTEMLVDLIQHGEKIDYCLTAGPIPMMKKVSEITKEHNIKTIASLNPIMVDG
                     TGMCGCCRVSIGGETKFACVDGPDFDAHLVNFDELLKRTSFYKEMEKISYEKFINSCK
                     CTSHQQK"
     gene            complement(10506..11042)
                     /gene="dcd"
                     /locus_tag="PLW95_06500"
     CDS             complement(10506..11042)
                     /gene="dcd"
                     /locus_tag="PLW95_06500"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HOV22311.1"
                     /translation="MVKSDKWIKMLGEKGMITPFFPEKIKKGISFGLSSYGYDFTLSN
                     EFLIFKGIGIVSPKKITKDQFDEFKGDVCKIKKNNFILGRSKEYFKIPRDVLGICFGK
                     STYARCGIIINVTPLEPEWEGYITIQIANLSSVDGEVYANEGIGQVIFISGEDICEKS
                     YKDLSGKYDKAKGIVLPK"
     gene            complement(11064..11135)
                     /locus_tag="PLW95_06505"
     tRNA            complement(11064..11135)
                     /locus_tag="PLW95_06505"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11101..11103),aa:Thr,seq:tgt)
     gene            11381..11503
                     /locus_tag="PLW95_06510"
     CDS             11381..11503
                     /locus_tag="PLW95_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22312.1"
                     /translation="MDIRYEKEFWKARSGKKREKILFIFNIGDIIIGNYKLKKK"
BASE COUNT         3386 a         2293 c         1563 g         4396 t
ORIGIN      
        1 cagggtctca tccagagggt tttctatcgc cagttgtccc acctgtcccg catcggccaa
       61 agccgccagt atgtcatccg gcttcacccc gccggaacaa aacaggaccc accagaccgc
      121 cacgctcccg accgcgacgg ccagtcccgc agccaccact ggaaccgaat tccgcataag
      181 ccttccgtcg agttcccccg taaatccgac gaaacacaac tacacagcca atgctccatg
      241 ctaccatact tcggtacccg ctttcaacat cggctcggga accttgagct tgaacttcgt
      301 aaagaacaac cgcatatact cggcacacaa aaaaagcccc gtcggttttg ccgacggggc
      361 ttgggtattc aagaaacagt taagcctgag ccgcgaaagc acctgacttt cgtccgtggt
      421 cccaaaactg accacaaacg tctaagtaat ccttagaaag gaggtgatcc agccgcaggt
      481 tcccctacgg ctaccttgtt acgacttagt gccagtcgtc ggacttaccg tcggcaggcg
      541 tctccttgcg gttaacaacc cgacttcggg tacttccaac tcccatcact tgacgggcgg
      601 tgtgtacaag gctcaggaac acattcaccg cgacatagct gattcgcgat tactagcgat
      661 tccaacttca tgcaggcgag ttgccagcct gcaatccgaa ctggggccgg ctttttgcga
      721 tttgctccac ctcgcggtct cgcgtcgctt tgtaccggcc attgtagcac gtgtgatgcc
      781 ctaggcataa aggccatgat gacttgacgt catccccacc ttcctccggt ttaacaccgg
      841 cagtcccgct agagttcccg gcatgacccg ctggtaacta acggcaaggg tttcgctcgt
      901 taagggactt aacccgacat ctcacgacac gagctgacga cagccatgca gcacctgtgc
      961 aagtttcact ccgaagagca gcttaacctg tttccaggca agcatccaag catgtcaaac
     1021 ctaggtaagg ttctgcgcgt tgcttcgaat taatccacat gctccaccgc ttgtgtgagc
     1081 ccccgtcaat tcctttgagt tttagccttg cgaccgtact ccccaggcgg cttacttaac
     1141 acttttgctt cgactgtgcc ggcgtcagaa ccgccacaat ctagtaagca tcgtttacgg
     1201 cgtggactac cggggtatct aatcccgttc gctccccacg ctttcgtccc tgagcgtcag
     1261 ttctgggata agaaactgcc ttcgccagtg gtgttcctgc cgatatctac acatttcacc
     1321 gctacaccgg cagttccgtt tctctccccc agactcaaag tcagaatgtc tcaactccaa
     1381 ttctttggtt gagccaaagg atttcagagt tgacgctcct gaccgcctgc ggacccttta
     1441 cacccagtaa atccgggtaa cgtttgtccc ctccgtatta ccgcggctgc tggcacggag
     1501 ttagccggga cttcctctgg aggtaccgtc atccgccata ggcggattct tccctcctga
     1561 caggggttta caacccgaag gctttcatcc cccacgcggt gtcgctgggt caccctttcg
     1621 ggcattgccc aagattcccc actgctgcct cccgtaggag tcggggccgt gtctcaatcc
     1681 ccgtgtggct ggccgtcctc tcagaccagc tacccgtctt tggcttggtg ggcttttacc
     1741 ccgccaacta cctgatagga ctcaggccca tcctctggca tttcccttgc gagaaatttt
     1801 tctcattcac catctggcaa atgagaatat caggtattag accacctttc gatggattat
     1861 ccctgtccag agggtagatt acccaagtgt tactcacccg tccgccgctt tccctaagga
     1921 aatctcacca taaggtaaaa tttctttagt tcgacgcacg acttgcatgt gttatgcaca
     1981 ccgccaacgt taaccctgag ccaagatcaa accctcagag taaattcaac cggatacccc
     2041 aaatttccaa agaacttttt ttacaaattt attttacctt cttactttca tgttgtcaat
     2101 acttattctg ctggtgcttc tggtgctggt gcttctggtg ctggtgcttc tggtgctggc
     2161 attccttctg gacccattgg tccttccatt gctggtggca ttgcttctgg tgttgccgct
     2221 ggttttttcg tcatctgttt aattataaaa aagagcgcca ccagaattat aattatcaga
     2281 attaccaatg ctgctggatt catctttttt tctgccattt tttctcacct ccttccattc
     2341 tataaaatat aaaacaaatt ttaatttttg tcaatggttt attttacctc aaatttgttt
     2401 aaatgtataa tatcttttaa atttcttttt ggaacatgca ttatttcttg ctcatctttc
     2461 caatatttta cacttccatt aaattcttca ataactgatt tttcagcagg atatcctaat
     2521 gcaataacag aatcaatgat ataatttccg ggtatttgta aaatttttct tacttgtttt
     2581 ctgtttactg aaccaatcca gcatgttcct attccttcat aagttgcaca aagaagaata
     2641 ttttcaattg cagcaccaca ttcaagttta tattttaaaa catttatttg tttattggca
     2701 attacaacaa tataagaaac aggccttttt ccttctggtg gttgtccctt ttctccaaga
     2761 tagccagccc atgcaagatt tgggaatatc tcatcaacaa cttctttttt attaacaaca
     2821 atatattcca atggttgtaa gttagaagca cttggtgcta atcttgccat gtcaataaat
     2881 tttataagaa gttcatttga tattggtttt tgttcaaatc ttctaattgt tcttcttttt
     2941 tttataactt cataaatatc cattctattt tctccctttc aaatcaatgc gtcctctaaa
     3001 aatcgtagcg cacgcccatt gaaaaacttc ggggtgcccg gattgtgcta tctgtaaatt
     3061 cccctatccc ctttcccctt tactaaaggg ggaaggatat ctccttcctt tgaaaaagga
     3121 aggatgggat ggatttcctg taatttcttt aaatccnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnngaaa ttcctcttta aatcccctct aactcccgtt gtgcctattg
     3301 aaatacatta cggcaccaac tctgctttgc tatcctttgc ttctttcgtc ggtttgaacc
     3361 catgttcaaa tcctctcaat gaagcgtgca agtcggggcg cccggatttg aaccggggac
     3421 ccccagaacc ccattctggt gccctaagcc agactgggcc acgccccgat taaaacctgc
     3481 cttctcttat tcccctgata tagttcatac aaaattcatc atttcctgtc aatagatttg
     3541 ttgtatataa tccctctttt atatcaacaa ttgtagggtc aattattact gaatcaatcc
     3601 cttcataaat acataaagaa agaaaatatt tattaacaat ttttcttttg ggaagtccaa
     3661 aagaaacatt tgataacccg caggttgttt ttacttctgg gacttcttct tttattttct
     3721 ttacacactt taagaaattc attgcatttt ttatatccac acttattggt tcaaccaaac
     3781 aatctataaa aatattttcc tttttcattc ctatctggga aagtgcatct attagatttt
     3841 ttgcaatttc aatttttttt tcaatagttt ttggaatacc attatcatcc attgttaaaa
     3901 caattatttt acattcagga aaccttttta ttatatcaag taattttttc tttttatctt
     3961 tctcaccatt tattgagtta attattatat cattattttc tacaaaagca agtgcaaatt
     4021 ttattacatc cgggtctgaa ctatcaatgc atattttaga aatattttct tcttttgtaa
     4081 tttcaatcaa ccatttaata tcctgttttt ctctttcttg tccttttcct gttcctgcat
     4141 ttaggtcaat ataggtagcg ccattctcaa tttgttttct tatttctctt tttatataat
     4201 ttttatcttt ttcttcaatt gcttttttta tatgttctct tgtgccattt attctttccc
     4261 ctattataat catctctctc ctattttggt atttatatta ttttaattat cctattacca
     4321 taaagaaatt ctccttctcc atcattttca actaattttt tcatatttga gacaacttca
     4381 ctccatttta aatattttgg taattctatt tcaattggta attgtcctga ataatttgat
     4441 ttttttaact cttttataaa ccatttccag tcaattgttc cttgaattgg tggaagatgt
     4501 aggtctcttt ctccatcatt atcatgcagg tggaatgtaa aaattttatc tttcagggca
     4561 ataaattctt ctttaaattt tcctatagta tgtgcatgcc ctgtatcaaa acaaacgcca
     4621 agatatggag aattaaattc ttcaattatt cttattattt cttcccctgt ccctatcatt
     4681 gtttcaggat atagnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnttttaa
     4801 aaatttttta tagtcatcta tctctttatc attaaaaaga agaaaatttc cataaacctc
     4861 actccatata tacttaatag aaacatcttt tagtatttct tcaatttcct cttttttaga
     4921 taaatcaaca attcttacag caatttcata ttttcccatt ttctcccttt attatatttg
     4981 caaattaaaa attcacggta ttagttaata ttgtaatata atatattaaa aatggaaatt
     5041 tcaaaagagg tatttgagga aataatatat cagaaattaa attgtttgcc gaaagagata
     5101 aaagataaac ttgagaatat atgttttatt gttgaggaaa atgccccttc atatacactt
     5161 cttggtcttt atcatggagt tccctatcct aaaagaggtc cttcttactc gcttgttttg
     5221 ccggatagaa ttatcctgta caaaaggaat attgaagcgg gttgtaaaac agaaaaagaa
     5281 cttataaaaa gaatagaaaa agttttgttt catgaaattg gacattattt ggggttagaa
     5341 gaaaagcaat taagaaaatt aggactataa atcaatgttt ttactaccag gttttaccag
     5401 tggtattccc cttttacata aaggacattc agatgaatta taatttttaa cttcaagttt
     5461 tacaagtgac cttaaaggat agttaaattc aattttacct ctttcaacta tacacccaat
     5521 tccttctatt tgtcctttaa ctttttcaac aatatcaatt atttctctaa cagaaccacc
     5581 tgttgttatt acatcttcac atataagaac cttctctcct ttttcaattt taaagcccct
     5641 tcttaatttc attttacctt cttctctttc tgcaaaaata ctacgtgctt tcaatattct
     5701 tgcaagttca taggaaatta ttattccacc aattgcaggt ccaataacaa catttatttt
     5761 tttctcttta aagttgtttg ctaagttctc acatagaatt tgaccatagt caggatactg
     5821 aaaaataatt gccatctgaa aatatgtatc tgaatggagt cctgatgaaa gaagaaaatg
     5881 tccagttaaa attgcacctg tttcttttaa aatttttaat atatcattca tttttttatt
     5941 ataacatttt ttaaggacat cgtcatctgt gcggactagt ccatactttt ttggctcacc
     6001 ttattttttt taaaaaaaat tgtttcttct ttcaaaattt ctatgtattt ttttataata
     6061 aaatcagtca tttcatttct attattttga aagaaatttt ttacttttcc aacgggcatt
     6121 attcccataa gggtatttgt aataaaaatc tcatcacact cacttaaagt tttagcagtg
     6181 aactttcctt cttttatttt tattttattt ttttgacata tttcaatgat tatttttctt
     6241 gttattccat ttaaaattcc acaatctaat gaaggagtat aaactatatt atttttcacg
     6301 agaaataaat ttgaaacagt tgtttcagta agaaaatcat tattgttcag aaaaattgta
     6361 tcttcacaac cattttcttt tgcttcaatt ttccctatta tattttcaag aaagttaaac
     6421 gactttattt ttgtgagagg cgacttttca tttcttataa ttttttcgct tataatacaa
     6481 cttattcctc tttcataaaa atacttagga taggaagtaa aattttttaa tataataaga
     6541 atatttgttt cttttccctc agggataaca ctttttcctt tttttctcca gacattaatt
     6601 cttatatatc cattttttat cctaaatttt tttaagtatt tcagtattga cttttcaatt
     6661 tcattttttg gtggtattcg aaaaccacag aaatctgcac cttttttcaa cctttcatag
     6721 tgttcaaaaa atctaaaaag tgagtagttt tttgaaataa ttgtttcaaa tataccatcc
     6781 ccaaagagaa atcccctgtt taaaattggc aaaccaatat cttttttttg ttttactttt
     6841 ccattaacta aataaatttc cggcattttg gtcaattccc aatgtaagaa ataaattctt
     6901 cgtttttata agtgtttctt catattcttt ttcagggata ctatcccaga caattccgcc
     6961 accaacagga taataaattt tattctcttt atgcaaaatt gttcttatta aaatgttaag
     7021 gagcatattt ttattaaatc ctatatatcc aagcgaacca gtataaaaag accttttttc
     7081 tggctcaagt tcttctatta tttccattgc tcttatttta ggtgcccctg taattgaacc
     7141 accgggaaat atatttttta ttatatcaac aggagctact tcttttttta atattccttc
     7201 aatagtagaa gttgtctgga aacatgtttt gtatttttct atttcaacaa gtttttttac
     7261 tttaatacta ccaaattcac atatttttcc gatgtcattt ctttcaaggt caacaatcat
     7321 aataagttcg gctctgtcct tttcactatc taataattcc tttttatttt cagagtcctc
     7381 ttttctgttt ttccctcttg atatagtacc tttcattggt tttgttatta tttttattcc
     7441 atattttttt ataaaaagtt caggagaatt ggaaagaatt tgaaagttat taaagtcaaa
     7501 atatgcagaa tatccagaag gatttatttt tctaattttg agataaattt ctgatggatt
     7561 aaatatccct tctgcttgaa atctttggga gaaattaatc tgataaacat ccccctgttt
     7621 aatatagttt tttatttttt caattgaact gacatattca gaaaatttca tattggaaga
     7681 aaaattttta attttagctt ctttttcatc attttgaaat ttaagagaat tttttatttc
     7741 tgatgaaatt aaagaaattt tctttttgaa tgtgctttct ttctcaaaaa aatttattcc
     7801 agttaagata agtttttttt taataatatc aaatataaga aaagtgtcgt aaaagcagaa
     7861 aaaaatatcg ggtattttta aaatatccgg atttatgtct ggtaaatttt caaatttcaa
     7921 ccctgcatca taacccaaat acccaactgc ccctggaata aagaaaaaac gagtatctac
     7981 ccagtatttt tcaagaagtt cattcaggta atcaaaaggt tttcctatat aagttttttt
     8041 tgaattggtg tcttctattg ttattttatc tcctttgctt ttaaaaataa gaaatggttc
     8101 aaaaccaact attgaatatc taccgtaaga tggtgaaaca ctatcaagga aaaagaaaaa
     8161 ttttttggta tggaatgact tgaaaatgag ctcaggaggt aggtaattta ggtattcaaa
     8221 gaaaatattc atcatttttc aagtttttta attttattaa ctctttttaa atgtctccct
     8281 tttaaaaatg gtgtttcaaa aaatgtcttt gaaatttcaa gagataaagt atttcctaac
     8341 aatcttcctc cgagtgttaa aatatttgca tcattatgtt gcctggcgag aagtgcatca
     8401 taaattgtac aacatcttgc tgctcttatt cctttaaatt tatttgctga tatactcatt
     8461 cctattcctg ttccacatat taaaatacca aattcaattt ctttccttat tattcctgat
     8521 gcaacttttt cagcaaagtc cggatagtca actacttttt taatttcagg tccatagtca
     8581 attatttcat aaccatcatt ttcaagttct ttttttaaaa agtccttcat ttcaaagcca
     8641 gcatggtcac ttgctatacc tattttcata ttgccatcct ttttaaaaca atatttttga
     8701 ctttctcttt tattatatct cttatttctt cataatgaaa aagttctttt cctgctgggt
     8761 ctggtacatc taaaacaaat attttatttt cttctttcgg aataattgat aaaatttttt
     8821 ctttttgttc tttttccata acaaaaataa ggtctgactc ttttaacatt tggaaattta
     8881 atggttttga aacaaaattt ttaatttcta ttccaacttt tttcatcact tcaagtgttt
     8941 tatcagatat tttaccccaa gatgttattc cagttccagc agaatttact tcaattttaa
     9001 catttttttc ttttaagaaa ttttttaaaa aatattcagc cataggactt ctacaggaat
     9061 tgccttcaca aacaaaaatt acttttcctc ttacaagttt aggtggagaa aaagatacat
     9121 cccagattgt tgattctttc ccttctgttt ttccaccatt aataattaaa tccactttgt
     9181 ttttaacttc atcagatatt tcagatagac aggaaggtgt tttttcgcct gatatatttg
     9241 cacttgttga ggcaataatt tcaaaatttt ttaaaacatc aagaagaaat ttattatcag
     9301 gaattcttag tcctattttt ttctcatcag aagttagaaa tggaatttca attttacttt
     9361 ttccaattat tgttgtactt cctggccatt tttcttttat aaactggcgg tcaatttcta
     9421 tatatttttc tgctttttct attgtatcaa caaatccaat taacattttt ttataatccc
     9481 tttttttaat ttcatatatt ttttctttgc ttctgtcatt aaatccacct gatatcaatc
     9541 cataaactgt atctgttggg acaataacaa ctccaccctt tttaagggta tttaccacaa
     9601 ctttaatatt ttcttctttc tcttctaata tcttttccat ttttttattt ttgttgatgg
     9661 gaggtgcact tacaggaatt tataaatttt tcataagata ttttttccat ttctttataa
     9721 aaagaagtcc tttttaaaag ttcatcaaaa ttaactaaat gcgcatcaaa atcaggtcca
     9781 tcaacacatg caaattttgt ttctccacca atagagaccc tgcaacaccc acacattcct
     9841 gttccatcaa ccataattgg atttaaactt gcaattgttt ttatgttatg ttcttttgtt
     9901 atttctgaca cctttttcat cataggaatc ggacctgctg ttaaacaata atcaattttt
     9961 tccccatgtt gtattaaatc aacaagcatt tcagtattaa atccttttct gcctttactt
    10021 ccatcatctg ttgtaatata aatttcatca cttatatttt ttaattcttc ttcaagtatt
    10081 attaaatcct tattccttgc accaattatt gtaattagat aattttcttt ttctttaaat
    10141 gtttttgcca tccagaatat aaaagcagct ccaactcctc ccacaataat acatacattt
    10201 ccatatttct caatttctgt tgcttttcca agaggaccca caatatctaa aatattttct
    10261 ccattttcta aaaatcctaa ttttgctgtt gttgttccaa cttcctgaaa tataagagtt
    10321 attgtttctt tttctttatt tacatctgca attgttaatg gtattctttc tcctttttcg
    10381 tcaactctta aaacaataaa ttgtcctggt tttgcttttt tagaaataat aggagagata
    10441 acttcaattc ttttaatttt ttctgctaat tgttcttttt ttattatttt attcattttt
    10501 taaaattatt ttggaaggac tattcctttt gctttatcat atttaccaga aaggtcctta
    10561 tatgactttt cacaaatatc ttctcctgaa ataaaaataa cctgcccaat tccctcattt
    10621 gcatagacct ctccatcaac agaagaaaga tttgcaattt gtattgttat atatccttcc
    10681 cattctggtt ctaatggtgt tacatttatt attattccac atcttgcata agttgatttt
    10741 ccaaaacaaa ttccaagaac atctcttgga attttaaaat attctttact tcttccaaga
    10801 ataaaattat ttttttttat tttacataca tcacctttaa attcatcaaa ctggtctttt
    10861 gttatttttt taggggagac aattccaatt cctttaaaaa taagaaattc attagataat
    10921 gtaaaatcat atccataaga agacagccca aaagaaattc ctttttttat tttttcagga
    10981 aaaaaaggag taatcattcc tttttctcct aacattttta tccatttatc tgactttacc
    11041 atttttttat ttgaaaataa taaagccacc tcggggactc gaaccccgga cccgcgcatt
    11101 acaagtgcgc cgctctgccg ctgagctaag gtggcaaact cttgctattt taataattta
    11161 tgcaatattc ccttttatcc tgaacatatt agtcagcatt ccatttcttt ctctctttta
    11221 taaatttctt tttaattata cccttttcat ctccattttt caaaatttat tgattcaaat
    11281 gtattttctt ctataaattt attcaaacat tcaatatcaa acattaattc ttcccaattt
    11341 attacttttg ggttatccct aaaaatgata aaatgtggct gtggatataa gatatgagaa
    11401 ggagttttgg aaagcaagaa gtggtaaaaa aagggaaaaa attttattta tctttaatat
    11461 tggagatata ataataggaa attataaact gaagaaaaaa taaataaatt actttttttt
    11521 gcttgttaaa aaaatttgcc atttttaaaa gtatgtttta aaatatcttt agataattaa
    11581 atttagtggc taaaattaaa gataatccaa aaattttagg gctttaaaaa taaaaactat
    11641 cacacaatca tgtggtaata acaatgtatt gacagaaaaa aagaaggggt aaaataatag
    11701 taaaagagga aggaaaatga tgaaaagaat agtaggaagt aataatagag aagaagtaat
    11761 agtcaagaaa aagaacacac acctagtatc tttttgtttt ttccttataa tcacaatttc
    11821 ttttttaatc attacaaa
//
LOCUS       DAOOVW010000046        11821 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_3548_length_11821_cov_9.145164, whole genome shotgun sequence.
ACCESSION   DAOOVW010000046 DAOOVW010000000
VERSION     DAOOVW010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11821
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3548_length_11821_cov_9.145164"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PLW95_06515"
     CDS             complement(<1..198)
                     /locus_tag="PLW95_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOV22313.1"
                     /translation="MIKTESNDYKDRTYLSAIEAAKYLGISVETLHNLVNKFIKAQVS
                     ASGQMRFDLKELKKYEEKFKNK"
     gene            complement(380..1345)
                     /locus_tag="PLW95_06520"
     CDS             complement(380..1345)
                     /locus_tag="PLW95_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020279282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOV22314.1"
                     /translation="MKLGVFLVLFGDKNLEEALDKAKELGLDAVEIGTGGYPGDAHCK
                     VNQLLEDEGKLKNFKNAFEKRGLEISALSCHGNPLHPDEKIATAHREIQRKTIQLAEK
                     IGVPRINTFSGCPGDSENSKYPNWVVCPWPDDFSAILKWQWEKKVIPFWKEEVEFAKK
                     HNLKEICFEMHPGFVVYNPETLLKLRKEVGEEIGANFDPSHLFWQGIDPLAALKKLKG
                     AIYHVHAKDTKINPYNTAINGVLDTKTYLDEVNRSWIFRTVGYGHGTEFWNDFVSTLR
                     MIGYDGVLSIEHEDSLMSTEEGLKKAVEFLKSVLIRQPKPKVWWT"
     gene            complement(1370..2134)
                     /locus_tag="PLW95_06525"
     CDS             complement(1370..2134)
                     /locus_tag="PLW95_06525"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOV22315.1"
                     /translation="MGKQIAGVQLYTLRDFCKSVSDTVNTFKRIKDMGYNVVQISAVS
                     EPQDVVELKKILDDTGLIPCSTHTAYEKIVNEIDRVIEEHKILGCNAIMCPGLPLQLH
                     NKEGYLKVASEFEKVIPKIKENGLIVGYHNHGIEFEKYDGKIGLEILLDNCEELEAEI
                     DTYWVQYGGGDPTFWIEKFSGRVSEVHFKDMGIIKNQQVMPPIGEGNLNWDRIVKCCK
                     KSGVKYCLVEMDNCTIDPFDGLKVSLENMKKWGIKV"
     gene            complement(2150..2959)
                     /locus_tag="PLW95_06530"
     CDS             complement(2150..2959)
                     /locus_tag="PLW95_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019908025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOV22316.1"
                     /translation="MKIGVIPDCFRVPIKEGIRKAAELEIEGIQPYATHGELDPKNLT
                     KTGRQDFKNFVSSLGLTISALVGDFGGHGFADAETVEWRLERTKQVIDLAVDLGVNIV
                     TTHIGVVPEDENDPAWKTMEESLPEVGNYASNKGVFLATETGPEPATLLKKLLDKLSN
                     PGIRVNYDPANLVMVAGDDPVKGVYTLKDYIVHTHAKDGIKLPDENGQKRWKEMPLGQ
                     GKVDWENYLKAMKEIGYNGFFTIEREVGENPEVDIILARDFLREISKKVGI"
     gene            complement(2937..3179)
                     /locus_tag="PLW95_06535"
     CDS             complement(2937..3179)
                     /locus_tag="PLW95_06535"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HOV22317.1"
                     /translation="MFEIKVKNEKCKGCKLCIEVCPKNVFKISTHFNKLGVHYAIVDK
                     ENNCNGCKRCVIICPDIAIEIYQKEKGGKNEDRGNT"
     gene            complement(3179..3958)
                     /locus_tag="PLW95_06540"
     CDS             complement(3179..3958)
                     /locus_tag="PLW95_06540"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOV22318.1"
                     /translation="MINSVFILENKKIEGIEKEKEKTEDIVRNCGKVVEKDIKNVDFL
                     ITMGGDGTILKGFHLLENKETLIYGIKYGKVGFLTNSNENIEEKLKKVFAGNFYVSER
                     TLLEIEIRSGNEFYKDICLNEIVVFRKGIRIINLSVETEKEKVLCFRADGIICSTPTG
                     STGHSLSAGGPILDPEIISFLIIPLMPHLLTTRPVILGKKDRIKISINGKVGIVIDGQ
                     KEYITEENSEINVFLSEKKGKIVLEDKKFFEKLSSKFHWSK"
     gene            complement(4206..6029)
                     /gene="dxs"
                     /locus_tag="PLW95_06545"
     CDS             complement(4206..6029)
                     /gene="dxs"
                     /locus_tag="PLW95_06545"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOV22319.1"
                     /translation="MKIIEILNENPSELKNLNSEGLENLANEIRETIIETVSKNGGHL
                     ASNLGVVELTIALHYVFNIPPDKIIWDVGHQCYTHKILTGRYKKFNTIRTPDGLSGFP
                     SPDENNADVFFTGHAGTSISSSTGLKYGLEKQEGNSKVISVIGDGSLTNGLTFEGLNF
                     LGSVKKNVIVILNDNRMSISPTKGALSYYLTKLITSPILNRPKEEFTEIIKKFPGVGD
                     DLLKIASDIEQKAKFLIVPGVFFEKLGLRYLGPIDGHDIIQLVDILENIKEIQQPVVL
                     HIITKKGKGYEFAEKNPCLFHSASPFDIKTGEFIKEEKNTPSIFTGNLLEKMAEENEF
                     FVITAAMEIGLGLENFAKKFPERFFDVGIAESHAIVLAGGIAKTGMKVIVSIYSTFLQ
                     RGFDQIFHDICLQKLPVIFLVDRSGIVGEDGPTHHGNFDISFLRVLPNIKIFAPYSLK
                     NIEEIIRNSFKETSTPTFIRYPKGHLPEDIEKERGDKKRKITLLGVGSMAEITHNVCD
                     ILNSENIPCLSIAIDRIKPLDDKIDGYLNSDVIATFEENTTIGGFGSSIAEYIADKRV
                     KRNLLIFGLPDKYIEQGDRKQLLDKYGLSERKIVDKIRGIL"
     gene            complement(6016..7371)
                     /locus_tag="PLW95_06550"
     CDS             complement(6016..7371)
                     /locus_tag="PLW95_06550"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOV22320.1"
                     /translation="MKKIIFPLLVIFLFSGCSTLPIKKEPVLKNYAKGVVAYQQENYD
                     EAIKFFEKEIKENGQSSYLNLKLGNLYLEKRMPDKAKKYFYRAKKKNPNDPDVYFGLG
                     LAYLFEKNNEMATYFIEKGLERDPGKTSFRLILSDIYVTLQNYEKGELHYSVLVNQFP
                     SNYLLHYNYAKLLEKTGKENLAEIEYLKTLDIVPFFWQANLSLALLYQKQGKNEKAKQ
                     FFENTIQYNPKNPVPYIFLSSIYYSEEKIDSAIEILEKGIGQGLKEKMIFDFLGSLYY
                     QKEEYNKAIENFNKSLEISDTSDTRFRLGILYDKIGQKQQMEEQMKKAIQLNPENPLA
                     LNYLGYSYLDENKNIKEAYKMIKKAVEKDPKNGAYLDSLGWAYYKMGNYKKARYYLEK
                     AINYEQDPEIEEHLAWTYFKLCNWEKALFYFTKAYEKTGKENILKEIEKIKCLIKNEN
                     H"
     gene            complement(7587..8465)
                     /gene="hisG"
                     /locus_tag="PLW95_06555"
     CDS             complement(7587..8465)
                     /gene="hisG"
                     /locus_tag="PLW95_06555"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOV22321.1"
                     /translation="MKKKKLKLVLPKGSLQETTFTIFKKAGFEIFSTSRSYFLSVDDP
                     EIECVLLRTQEIPRYVELGTFDTGIAGKDWITENRADVIEVCELVYSKQGFMPVKIVI
                     AVPEKSNIRKVEDLEGKLIATELVNLTNDFLKKRGIKAKVEFSYGATEIKAGKVVDAI
                     VEITETGSTLFSHNLKIIEVIMESTPRLIANKDAWKDNWKREKIKNISLLLNGALISE
                     GKVGLKMNLKKEDLSKIIKILPAIKKPTISPLADEKWVAIETIVDKNIVKDLIPKLKK
                     AGAEGIVEYSLNKVIY"
     gene            complement(8446..9414)
                     /locus_tag="PLW95_06560"
     CDS             complement(8446..9414)
                     /locus_tag="PLW95_06560"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002685762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HOV22322.1"
                     /translation="MRKKYLPFEKPIEEIERRIEQLEKARKNGSSEVGGEILKLKYQL
                     IEKTKEIFSNLTAWQIVQIARHPERPHTQDYINKIFDDFFELHGDRISGDDTSIIAGI
                     GKFKGITVTIVGHQKGKDTKENIERKFGMAHPEGYRKARRVMKLAEKLHLPVITFIDT
                     PGAQPDIEAEEKGQALTIAENLLEMSLLKTPIICIIIGEGGSGGALAIGVGDRILMQE
                     FAIYSVISPEGCASILWKNQNAVEDAAKSLKLTAKEIYQLGIIDEIISEPIGGAHSNW
                     DETFKNMEGAIDKNLRELKTIDIEDLVELRYQKYRKIGALDEKEKT"
     gene            complement(9416..9709)
                     /gene="gatC"
                     /locus_tag="PLW95_06565"
     CDS             complement(9416..9709)
                     /gene="gatC"
                     /locus_tag="PLW95_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008099983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOV22323.1"
                     /translation="MEKKIIKKDDVDYLGDLARISLNEQEKEKICSDLEKILNYVSQL
                     EKLDTEDVEPTYHSHPVKNVFRKDIVERHLSNDEILKNAPERKGDFFKVPKII"
     gene            complement(9879..10001)
                     /locus_tag="PLW95_06570"
     CDS             complement(9879..10001)
                     /locus_tag="PLW95_06570"
                     /inference="COORDINATES: protein motif:HMM:NF026853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22324.1"
                     /translation="MLVDIRIGQYSNGKKHDHGTGFRVLPSKLDLCFKHREKVL"
     gene            complement(9961..10434)
                     /locus_tag="PLW95_06575"
     CDS             complement(9961..10434)
                     /locus_tag="PLW95_06575"
                     /inference="COORDINATES: protein motif:HMM:NF026853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MvaI/BcnI family restriction endonuclease"
                     /protein_id="HOV22325.1"
                     /translation="MIELKSARKNATSMLTLFTKSPLPPKANSILLERFGYESGKDNK
                     KKELHTTVNAKEFNQLKGKAGFKIDIQEDNINIITPENEIVGYWDKETLKKSFEKKLP
                     KLLYVKAESRGRGENEEFWFNEAWLLSGFNFRKFCPSIKRRNNISRYKNRTILKW"
     gene            complement(10473..10586)
                     /locus_tag="PLW95_06580"
     CDS             complement(10473..10586)
                     /locus_tag="PLW95_06580"
                     /inference="COORDINATES: protein motif:HMM:NF026853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MvaI/BcnI family restriction endonuclease"
                     /protein_id="HOV22326.1"
                     /translation="MKEYFLLIEKLQEIKEKGYIKTHRKGNTGIGKTLEIF"
     gene            complement(10583..11488)
                     /locus_tag="PLW95_06585"
     CDS             complement(10583..11488)
                     /locus_tag="PLW95_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014406950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOV22327.1"
                     /translation="MSDIKIREEKKNSKKLDIDYKRKCNCPKSHINCLTPKEWVKGQV
                     AIWEFYYEKRDIRDKDIHPAVFPIALPKKCIELFTHKGELVLDPFVGIGTTLIAAKDL
                     GRNAVGFDLNQKYIDFANERLQNPDLFISNDETKQIAICDDAINIPNYLENTTIALCV
                     TSPPYANMLNHERLNKSLRSDLRNNKHYKKIQQYSNHPNDLGTMKLTDYIQALSEIYR
                     QILPLLKPKAHCVININDLWENNHRYPTHSYIIEAMEEIGYKLRNIIIWDKRNLVNKV
                     GIFGYPSNYITLSTTFEYILDFWRP"
     gene            complement(11562..11652)
                     /locus_tag="PLW95_06590"
     tRNA            complement(11562..11652)
                     /locus_tag="PLW95_06590"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11616..11618),aa:Ser,seq:gct)
BASE COUNT         3607 a         2134 c         1396 g         4684 t
ORIGIN      
        1 tttattttta aatttttctt catacttttt tagttctttt aaatcaaatc gcatttgtcc
       61 acttgcagaa acttgtgctt taataaattt atttacaaga ttatggagtg tttcaactga
      121 aatccctaaa tattttgctg cttctattgc tgaaagatag gttctatctt tatagtcatt
      181 tgattctgtt tttatcattt tttgccttaa tagtgtctat acaaaaaagt tatagataaa
      241 tatattattt atatttattt tatcaatttt gtaaaataat tcaaaaagga atgtttgaac
      301 tgcgaagaca ataacaaaaa aattatttct tatattctta actctaaaat tttaacaaaa
      361 tataaattaa attggaaaat tatgtccacc aaacttttgg ttttggttgt cttattaaaa
      421 cactttttaa aaactcaact gcttttttca gtccctcttc tgtgctcatc agggaatctt
      481 catgttctat actcaaaaca ccatcatatc caatcatcct taatgttgaa acaaaatcat
      541 tccagaattc agttccatgt ccatatccga ctgttctaaa aatccaactt ctatttacct
      601 catctaaata tgtttttgta tcaagaacac catttattgc tgtattgtat ggatttattt
      661 ttgtatcttt tgcatgaaca tgataaattg cacctttaag tttcttcaaa gcagcaagtg
      721 ggtcaatccc ctgccagaat aaatgagatg ggtcaaaatt tgccccaatt tcttctccaa
      781 cctcttttct taattttaaa agtgtttctg gattataaac aacaaatcca ggatgcattt
      841 caaaacaaat ttcttttaaa ttatgctttt ttgcaaattc cacttcttct ttccaaaatg
      901 gaataacttt tttctcccat tgccatttta aaattgcaga aaaatcatct ggccaggggc
      961 aaacaaccca gtttggatat ttgctatttt cactatcacc aggacatcca gaaaaagtat
     1021 ttattcttgg cactcctatt ttttcagcca gttgtattgt ttttctctgt atttctctat
     1081 gagcagttgc aattttctca tctggatgca acggattgcc atgacaggat aaagcagata
     1141 tttcaagccc tcttttttca aaggcatttt tgaaattttt taatttacct tcatcttcaa
     1201 gcaattggtt cactttacaa tgagcatctc ctggatatcc acctgtccct atttctactg
     1261 catcaagtcc taattctttt gctttgtcaa gtgcttcttc taaattctta tcgccaaata
     1321 gaactaaaaa aacccccaat ttcatttttt acctccagtt tttattagat taaaccttta
     1381 ttccccattt tttcatattt tctaaactta cttttaatcc atcaaagggg tcaattgtac
     1441 aattatccat ttcaactaaa caatatttaa cacctgattt tttgcaacat ttaacaattc
     1501 tatcccaatt caaatttcct tctccaatag gaggcattac ctgttgattt tttattattc
     1561 ccatatcctt aaaatgaact tcacttactc ttcctgagaa tttctcaatc cagaaagttg
     1621 ggtctcctcc accatattga acccagtaag tatctatttc cgcttcaagt tcttcacaat
     1681 tgtcaagcaa aatttcaagc cctatttttc catcatattt ttcaaactct attccatgat
     1741 tgtgatatcc cacaataagt ccattttctt taatttttgg aataactttt tcaaattcac
     1801 ttgcaacttt taaatatccc tctttgttat gaagttggag aggtagtccc ggacacatta
     1861 ttgcattaca tccaagtatt ttgtgttctt ctattaccct gtcaatctca ttgacaattt
     1921 tttcataagc agtatgagtg gaacagggta taagacctgt atcatcaagt attttcttta
     1981 attcaactac atcttgtggt tcagaaacag cagaaatttg aacaacatta tatcccatgt
     2041 ctttaattct cttaaatgta tttaccgtat ctgaaacact tttacaaaaa tcccttaaag
     2101 tataaagttg aactcctgca atttgttttc ccatttttat catctccttt tatataccaa
     2161 ctttttttga aatttctctt aaaaaatctc ttgctaaaat tatatcaact tccgggtttt
     2221 ctccaacttc tctttcaatt gtaaaaaatc cattataccc tatttctttc attgctttta
     2281 aatagttttc ccaatcaact ttaccctgtc caagaggcat ttctttccat cttttctgtc
     2341 cattttcatc aggaagttta atcccgtctt ttgcatgagt gtgaacaata tagtccttta
     2401 aagtataaac acctttgaca gggtcatcac ctgcaaccat aacaagattt gcagggtcat
     2461 aattaactct tattcccgga tttgataatt tatcaagaag tttttttaat aatgttgctg
     2521 gttcaggacc tgtttctgtt gcaagaaaga cacctttatt ggaagcataa tttcctactt
     2581 ctggcaatga ctcttccatt gttttccaag cagggtcatt ttcgtcttca ggaacaactc
     2641 caatatgagt tgttacaata ttaactccaa ggtcaacagc aaggtcaatt acctgctttg
     2701 tcctttcaag acgccattca actgtttctg catctgcaaa accatgccct ccaaaatcac
     2761 caacaagagc agaaattgta agtccaagag aagaaacaaa atttttaaag tcctgtctgc
     2821 ctgtctttgt cagatttttt gggtcaagtt ctccatgagt tgcatatggc tgtattccct
     2881 caatttctaa ttctgctgct ttcctaattc cttctttaat cggcactcta aaacaatcag
     2941 gtattacccc tatcttcatt tttgcctcct ttttcttttt gatatatttc aattgcaata
     3001 tcaggacata ttataacaca tcttttacaa ccattacaat tgttttcctt atcaacaatt
     3061 gcataatgaa cacctaattt attgaaatga gtagaaattt taaaaacatt ttttggacac
     3121 acctcaatac aaagtttaca acccttacat ttttcatttt ttacttttat ttcaaacatt
     3181 atttgctcca atggaacttt gaagagagtt tttcaaaaaa ttttttatct tcaagaacaa
     3241 ttttaccttt tttttcagaa agaaagacat ttatttctga attttcttct gttatatatt
     3301 ctttttgtcc atcaataaca ataccaactt ttccatttat gcttatttta attctatctt
     3361 ttttacctaa aataacaggt cttgttgtaa gaagatgtgg cattaaagga ataatgagaa
     3421 aagaaataat ttctgggtcc aaaataggac ctcctgctga taaagaatgg ccagtggaac
     3481 cagttggagt tgaacaaata atcccatctg ctctaaaaca aagtactttt tctttttctg
     3541 tttcaacaga aaggttaata attcttatac cttttctaaa aacaactatt tcatttaaac
     3601 aaatatcttt ataaaattca ttcccgcttc ttatttcaat ttcaagaagt gtcctttcag
     3661 aaacatagaa attaccagca aaaacttttt ttaatttttc ttcaatattt tcatttgaat
     3721 ttgtcaaaaa accaactttc ccatatttta ttccataaat taatgtttct ttattttcaa
     3781 gtaaatgaaa tccttttaaa attgttccat ctcctcccat agttattaaa aaatccacat
     3841 tttttatgtc tttttccaca acttttccac aattccttac aatatcctct gttttttctt
     3901 tttctttctc aattccttct attttcttgt tttctaaaat aaaaacacta tttatcattt
     3961 acaattccct ctaaatatcc actgaacatt tgtatttcag gcataaaaat taaactcccc
     4021 cttttttccc gttatgtttg gaaattcctc tcaaaatccc ccttaacccc ctttgtcaaa
     4081 gggggaattt tgggaaaaaa gcaccaactc tgctttgcta ccctttgctt ccttagtcgg
     4141 tttgttaaag gggaaacttg taagtaagaa atatcttctc tatggtcttt ttgttttacg
     4201 aggatttaca aaattcctct tatcttatcc acaatttttc tttcacttaa tccatattta
     4261 tcaagtagtt gttttctatc tccctgttca atatatttat caggtaatcc aaatattaaa
     4321 agattccttt ttactctttt atctgcaata tattcagcaa ttgaagaacc aaagccacct
     4381 atggttgtat tttcctcaaa agttgcaatt acatcactat tcagataacc atctatttta
     4441 tcatcaagtg gttttatcct atcaattgca attgacagac acggtatatt ttcagaattt
     4501 aaaatatcgc atacattatg agtaatttct gccatacttc caacacctaa aagggttatt
     4561 tttctttttt tatctcctct ttctttttca atatcttctg gtaaatgtcc cttgggatac
     4621 cttataaaag ttggagtact tgtttcctta aatgagtttc ttataatttc ttcaatattt
     4681 ttcagagaat aaggagcaaa aatttttatg tttggtaaaa cccttaaaaa agaaatatca
     4741 aaatttccgt gatgtgtagg gccatcttct ccaacaatac cacttctatc aacaagaaaa
     4801 ataacaggta atttttgtag acatatatca tgaaaaattt ggtcaaaccc tctttgtaaa
     4861 aaagttgaat aaatagaaac aataactttc attccagttt ttgcaatacc acctgctaaa
     4921 acaatagcat gactttcagc aattccaaca tcaaaaaatc tttcagggaa tttttttgcg
     4981 aaattttcaa gtccaagccc aatttccata gcagcggtta taacaaaaaa ttcattttct
     5041 tcagccattt tttcaagaag attacctgta aaaattgatg gagtattttt ttcttcttta
     5101 ataaattctc ctgtttttat gtcaaaagga gaagcactat ggaataaaca tggatttttt
     5161 tctgcaaatt catatccttt accttttttt gttattatat ggagaacaac tggttgttga
     5221 atttctttta tgttttcaag aatatctacc agttgaataa tatcatgtcc atcaattggt
     5281 cctaaatatc tcaagccaag tttttcaaaa aaaacaccag gaacaataag aaattttgct
     5341 ttttgctcaa tatcagatgc aatttttaat aagtcatctc caacacctgg aaatttcttt
     5401 attatttcag taaattcttc cttaggtctg tttaatatag gagaagttat aagttttgtt
     5461 aagtaataag aaagcgctcc ttttgttggg gaaatagaca tcctattatc atttaaaata
     5521 acaataacat ttttctttac tgacccaagg aaattcaaac cttcaaaagt tagtccattt
     5581 gtaagagagc catctccaat taccgatatt acttttgaat tgccttcttg tttttcgagc
     5641 ccatatttta aaccagttga agaagaaata gaagtgccag catgtcctgt aaaaaaaaca
     5701 tctgcattgt tttcatcagg agaagggaaa ccgcttaatc catcaggtgt tcttattgta
     5761 ttgaattttt tatatctccc tgtcaggatt ttatgagtat aacattggtg tccaacatcc
     5821 catataattt tatcaggagg gatattaaaa acataatgaa gtgctattgt aagttcaaca
     5881 actcctaaat ttgatgcaag atgtccacca ttttttgaga cagtttcaat tatagtttct
     5941 cttatttcat ttgcaaggtt ttcaagtcct tctgaattta gattttttaa ttcagaagga
     6001 ttttcattca atatctcaat gattttcatt ttttatcaaa cattttattt tttcaatttc
     6061 tttaagtata ttttctttcc ctgttttttc atatgccttt gtaaaataaa aaagtgcctt
     6121 ttcccaatta caaagtttaa aatatgtcca ggcaagatgt tcctcaattt cagggtcttg
     6181 ttcataattt attgcttttt caagataata tcttgctttt ttataattac ccatcttata
     6241 ataagcccag cccaaactat caagataagc accattttta gggtcttttt ctactgcctt
     6301 tttaatcatt ttatatgcct cttttatatt tttattttca tcaagataag aataacctaa
     6361 ataatttaat gcaagaggat tttcaggatt taattgtatt gcttttttca tctgctcttc
     6421 catctgttgt ttttgcccga ttttgtcata taaaattcct aaacgaaatc ttgtatcaga
     6481 agtgtctgat atttctaaac ttttattaaa attctctatt gccttgttat attcctcttt
     6541 ttgatagtaa agagaaccta aaaaatcaaa aatcattttc tcttttaatc cttgaccaat
     6601 ccctttttct aaaatttcaa ttgcagagtc aattttctct tcgctgtaat atatacttga
     6661 aaggaaaata taaggaactg gatttttggg gttgtattga attgtattct caaaaaattg
     6721 ttttgctttt tcgtttttcc cctgtttttg ataaaggaga gcaagagata aatttgcctg
     6781 ccagaaaaaa ggaacaatat caagtgtctt taaatattca atttctgcta aattttcttt
     6841 tcctgttttt tcaagcaact ttgcataatt gtaatgaagg agataatttg aagggaactg
     6901 atttacaaga actgaataat gtaattcacc tttttcatag ttctgtaatg ttacatagat
     6961 atcagataaa attaacctga aagaagtttt tcccgggtcc ctttcaagtc ctttttcaat
     7021 aaaatatgtt gccatttcat tatttttttc aaaaaggtaa gcaagaccaa gtccaaaata
     7081 aacatcaggg tcattgggat ttttcttttt tgccctataa aaatattttt ttgctttatc
     7141 aggcattcgt ttttctaagt ataaattccc taattttaaa tttaaataac tactctgccc
     7201 attttctttt atctcttttt caaaaaactt tattgcctca tcataatttt cctgttgata
     7261 agcaacaaca ccttttgcat aattttttaa aacaggttct ttttttatag gtaaagttga
     7321 acaaccacta aataaaaaaa ttaccaataa aggaaaaata atttttttca tttatctttt
     7381 tcttttttta tgtcttaatg cttttcttct tttttttctt ttatgtgttt ttattttcat
     7441 cctttttctt tttttaccag atggcatatt ttctcctttt caattttata gattttataa
     7501 tatttttact ccaaaaaaca attttgctat tctttcaaag aaatatcttc ctactattaa
     7561 ttattaacat attttgtaag aagtgtttaa tatattacct tatttagtga atattcaaca
     7621 ataccttctg ctcctgcctt ttttaatttt ggaataaggt cctttacaat atttttatca
     7681 acaattgttt caattgctac ccatttttca tctgcaagtg gagaaatagt cggctttttt
     7741 attgctggta gaatttttat aatttttgat aagtcctctt ttttcaaatt catttttagt
     7801 ccgacttttc cttctgaaat taaagcacca tttaaaagca atgaaatatt ttttattttt
     7861 tcccttttcc agttgtcttt ccatgcatct ttatttgcaa ttaaacgagg ggttgattcc
     7921 ataattactt caataatttt tagattatga gaaaaaaggg tagagccagt ttcagttatc
     7981 tcaacaattg catcaacaac tttgcctgct tttatctcag ttgctccata ggaaaattca
     8041 acttttgctt ttatccctct ttttttaaga aaatcattcg ttaagttaac aagttcagta
     8101 gcaataagtt ttccttctaa atcttcaact tttcttatat ttgacttttc tggaactgca
     8161 ataacaattt tgacaggcat aaaaccctgt tttgaataaa caagttcaca aacctcaata
     8221 acatctgctc tattttctgt tatccagtcc tttcctgcaa taccagtatc aaaagtccct
     8281 aattctacat atcttggtat ctcctgagtt cttaaaagaa cacactcaat ttcagggtca
     8341 tcaacagata agaaataact ccttgaagtg gaaaaaattt caaaccctgc ttttttaaaa
     8401 atcgtaaaag ttgtttcttg caatgaccct tttggtaaaa caagtttaag ttttttcttt
     8461 ttcatctagc gctcctattt ttctgtattt ttgatacctt aattcaacga ggtcttcaat
     8521 gtctattgtt tttaattctc ttaaattttt atcaatcgct ccttccatat tcttaaatgt
     8581 ctcatcccag tttgaatggg ctcctccaat aggttcggat ataatttcgt caataattcc
     8641 taattgataa atttccttag cagtcaattt aagcgatttt gctgcatctt caactgcatt
     8701 ctggtttttc cataaaattg aagcacatcc ttcaggcgaa attacagaat atattgcaaa
     8761 ttcctgcatc aaaattctat ctcctacgcc aattgcaaga gcaccaccac ttcctccctc
     8821 tccaataatt atacatataa taggggtttt caaaagagac atttcaagta aattttcagc
     8881 gattgttaat gcctgtcctt tttcttctgc ttcaatatct ggttgtgccc caggagtatc
     8941 aataaatgta ataacaggta aatgaagttt ttctgcaagt ttcataactc ttcttgcttt
     9001 tctgtatcct tctggatgag ccatcccaaa ttttctttcg atattttctt tggtatcttt
     9061 ccctttttga tgtcctacaa ttgtaacagt tattccttta aatttcccta taccagcaat
     9121 tattgaagta tcatctcctg agattctatc accatgcaat tcaaaaaaat catcaaaaat
     9181 tttattgata taatcttgtg tatgtggcct ttctggatgc cttgcaattt gaacaatttg
     9241 ccaggcagtt aagttggaaa aaatctcttt tgttttctct ataagttggt atttcaattt
     9301 taaaatttct cctccaactt cagaagaacc atttttcctt gctttttcta attgctcaat
     9361 tcttctttca atttcttcaa ttggtttttc aaaaggtaga tatttttttc tcattttaaa
     9421 ttatttttgg gactttaaaa aaatcacctt ttctttcggg agcatttttt aatatttcat
     9481 cattagatag gtgtctttcc actatatctt ttctaaatac attttttaca gggtggctat
     9541 gataggttgg ttcaacatct tctgtgtcca atttttctaa ttgtgaaaca taatttaaaa
     9601 ttttttcaag gtcagaacag attttttctt tttcctgttc atttaaagaa atccttgcta
     9661 aatctcctaa ataatcaaca tcatcctttt taattatttt tttctccata ctttaagttt
     9721 atttcaaaaa aacaaaaaag tcaacttctt cacttctatg gtatttctca aaaataaaaa
     9781 tactaaaagt aaagttaaaa cagatttttg ttttttttgt ttcctaaatt gtattaaaaa
     9841 ttttaagatt tttgaaatgt aatcaatgga aactgcactt ataatacttt ttctctatgt
     9901 ttaaaacata ggtctaattt agatggtaaa accctaaaac ccgttccatg gtcatgtttt
     9961 ttaccatttg agtattgtcc gattcttata tctactaata ttgttccttc ttttaataga
    10021 tggacaaaat tttctaaaat tgaaaccact taataaccat gcctcattaa accaaaattc
    10081 ttcgttttca cctcttcctc ttgattctgc tttcacatat aataatttag gaagtttttt
    10141 ctcaaaagat ttttttagcg tttctttatc ccaatatcca acaatttcat tttcaggagt
    10201 tataatattt atattatctt cttgaatgtc aattttaaaa ccggccttcc ctttcagttg
    10261 attaaattcc ttagcattta ctgtggtatg taattctttc tttttattat ctttaccaga
    10321 ttcatagcca aatctttcta ataaaataga gtttgctttg ggcggtaatg gagatttagt
    10381 aaataaagtt agcatactgg tagcgttttt tctcgctgat ttcaattcta tcatttttgc
    10441 atttggtcca ggtatattat tttcctttat ccctaaaaga tctcaagggt ttttcctata
    10501 ccagtattac cttttctatg agttttaata tatcctttct cttttatttc ttgtagtttt
    10561 tcaataagca aaaaatattc tttcatggtc gccaaaaatc aagtatatac tcaaatgtag
    10621 tacttaaagt tatatagtta ctaggatagc caaaaatacc tactttattt actaaatttc
    10681 ttttatccca aataataata tttcttaatt tgtaacctat ctcctccatt gcttcaataa
    10741 tataagagtg tgtaggatac ctatgattgt tctcccataa atcgttaata ttaataacac
    10801 aatgtgcctt tggttttaac aaaggtaaaa tttgtctata aatttctgat aacgcttgta
    10861 tataatccgt taatttcata gtacctaaat catttggatg atttgaatat tgctgaattt
    10921 ttttgtaatg tttattattt cttaaatcac ttcttaaact tttatttaat ctttcatgat
    10981 tcaacatatt tgcataaggt ggtgaagtta cacataaagc aatagttgtg ttttcaagat
    11041 aattagggat gtttattgca tcatcacaaa tagcaatttg tttagtttca tcgttagata
    11101 taaataaatc tggattttgt aatctttcat ttgcaaaatc gatatatttt tgatttaaat
    11161 caaagccaac tgcatttctt cctaaatctt tagcagcaat taaagttgtt ccaataccaa
    11221 caaaagggtc taatactaat tctcctttat gagtaaaaag ttcaatacat tttttaggca
    11281 aagcaatagg aaaaacggca ggatgaatat ccttatctct aatatctctt ttttcataat
    11341 aaaattccca aattgctacc tgtcctttaa cccactcctt gggagttaga caatttatat
    11401 gggattttgg acaattacac tttcttttgt aatcaatatc taatttttta gagttctttt
    11461 tttcttctct tatttttatg tctgacattt tttaaccgtt tgtttatata ataccagata
    11521 agttaaaatt aagtaaagat ttttgttgtt tttaagataa gcggaggggg tgggagttga
    11581 acccacggta gcgcgtttcc acgctacaac ggattagcaa tccgccgcac taggcctcta
    11641 tgcgacccct ccttttactc attataccat ttacctctcc catattgcaa tttatcagca
    11701 ttccacaaaa ataatctcca agaaagagga tagtttcctc ataaataaat ttgataaaga
    11761 taaagtccat cctgtatata tgaataaaaa agaaaggaga tatacaggat ggtcacaaaa
    11821 a
//
LOCUS       DAOOVW010000047        11752 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_3570_length_11752_cov_8.724801, whole genome shotgun sequence.
ACCESSION   DAOOVW010000047 DAOOVW010000000
VERSION     DAOOVW010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11752
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3570_length_11752_cov_8.724801"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="PLW95_06595"
     CDS             complement(<1..992)
                     /locus_tag="PLW95_06595"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOV22328.1"
                     /translation="MPTKKEVINIIQNVGLWGRKEIELALEEQSKTGQPLKEIFQNRG
                     MLPFGEVAPSFYFQLGIVQRELSAREIPHEVLSLLPGKIAKSHRVVPWEKRENGVVVL
                     VTDDPINILAGDYFKGLINIEGIKDVEIMVTFKEEMDQLLEKYYGEGEMEDLTKMIQE
                     ASSATIDLDLPAEELGDDEESLALQAPVVKIVNLVFVEALRRRASDIHFEPLEKKFRV
                     RFRIDGVLYETVSPPKRIEKAVIARVKLMAKMDLAEKRLPQDGRIMLDISGRPIDFRV
                     SSLPGIYGESVVLRILDKTSMLKGLEELGFLEYDLKRWENLLKYSGGIVLVTGPT"
     gene            complement(1108..2277)
                     /locus_tag="PLW95_06600"
     CDS             complement(1108..2277)
                     /locus_tag="PLW95_06600"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOV22329.1"
                     /translation="MAKIIYNFPYKNYILFIDLFQGNITIKNVEFKFISEKKKADFET
                     FKKVLEGKEKAKRVHFAELLFDYEIKKYISEKYFGRKWIDFSYENIDDWTKQEIYFWY
                     QLGYDYARISGGLDFEGKGRVGEDTAVLSRGQRGWVEENKGVISNWDEFEKYPWPKIN
                     EIDYKTYEITSKNLPEGMKMVVCPSSGVFEISSEKILGFENMSYLIYENYELVEAVFN
                     KVGQLMVSFYENIVEIDNVEGFFQGDDLGFKTSTFLSPFYIRKLVIPFHKKFSQIAHE
                     KGKMYFLHCCGNVLEIMNDFIYDVKIDAFHSFQDEIIPVWEFKELYDEKIGILGGIDV
                     NKLCTYNEENLRKYVRRVLDQCMPERYALGSGNSIANYIPVQNFLIMLDEGWKYK"
     gene            2250..3620
                     /locus_tag="PLW95_06605"
     CDS             2250..3620
                     /locus_tag="PLW95_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOV22330.1"
                     /translation="MENYKLFSPSDYRYKVDQLIDYLTEESFLNYKIKVEIALLQTLS
                     DFNLCPEDVVKEVEKVARNIKAEEIYEEEKRIKHDIRAIVNVLKKNVSEKAKPFIHFC
                     ATSYDIVEPARCLMLKEATEKVIIPDMIELEDIFIKQARKEKKTIQIGRTHGQHAEPI
                     TFGFFLSVYIERWGERILKVNNAKENLKGKFSGPVGCYNGLSLFIKDVEKFESTFLEK
                     LSLKPSRISTQIVPPEPMVDFVHSIISSFSVLANLSDDMRNLQRTEIGEVGEPFEENQ
                     VGSSTMPQKRNPINFENVKSAWKTFMPRIITSYSDQISEHQRDLTNSLSQRYIPELLV
                     MFDSSIRRIKSIMEKIVVDKNNLVKNFNMSKDKIIAEPMQILLSFYGHPDAHEKIRQL
                     SMKSYQTGIPLTEIVFQDEEIKKYIEKFTPQQRKVIENPENYTGIASQKVNKICNFWE
                     KKLKRI"
     gene            3622..4659
                     /gene="purC"
                     /locus_tag="PLW95_06610"
     CDS             3622..4659
                     /gene="purC"
                     /locus_tag="PLW95_06610"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010878767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOV22331.1"
                     /translation="MGSVKDLEIIKKAEKDKMGIGRFHFSDRYSVFDWGEMPDNIDGK
                     GASLCIVSAFFFEKIERKGIKTHYRGVVDKNGNLRKFGEIERPVSTMEISLVRVLKPT
                     FSSGKYDYSIYKKEKGNFLIPLEIIYRNGIPSGSSILKRLKNGELNLSDIGLDHFPVE
                     GEKLEKPIFDISTKLEMKDRYISLKEGKEIACLSDEEVSQMQEVLFVINATISEEVKK
                     IGIENEDGKIELGFTPEREFIVVDVIGTPDECRFTYNGINLSKEIARQYYRKTKWYEE
                     VERAKNEAKDIGIDDWKSLCKLNPKKLPTKLKKTIENIYKSFANELTGKKFFASPPLK
                     EIIQEYSSYLP"
     gene            4934..5683
                     /gene="arsM"
                     /locus_tag="PLW95_06615"
     CDS             4934..5683
                     /gene="arsM"
                     /locus_tag="PLW95_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HOV22332.1"
                     /translation="MKKDIKQIVKKYYGKIATKNTGCCSSGCGCENTATNEQIAESIG
                     YSNQEIKSASDANLGLGCGNPTGMGQIREGDVVLDLGSGAGFDCFLAAKKVGISGRVI
                     GVEISEEMVERARSNANKYGYKNVEFRLGDIEELPVEDNSINIIISNCVINLAPDKSK
                     VFNEARRVLKKGGKMYISDIVLLKNLSKEQKGDNELIAGCVGGALLKNDYLKIIKKAG
                     LKIKVLSEDKDISKRHYQGIPLESLKIEIQK"
     gene            complement(5806..5898)
                     /locus_tag="PLW95_06620"
     tRNA            complement(5806..5898)
                     /locus_tag="PLW95_06620"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5862..5864),aa:Ser,seq:cga)
     gene            5950..6879
                     /locus_tag="PLW95_06625"
     CDS             5950..6879
                     /locus_tag="PLW95_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22333.1"
                     /translation="MNYLNYGIIEKEFPWIFEKQQQAIISPDVDGILCGLLMSNYLEW
                     EIVGFYDGKMLCQTKNISIKDCIFLDIEIFRKEIRSCGHHMLLYNKNEVPDNWGNFNK
                     SINPNNLRNFDALHNFQKKYPLATIHFLLCCLKEILNKEIKFLNTTTVPLLYVDGTFK
                     NLLNYPENCNEWLMFLKAKKQDSPIYPIFEIFSRQKLKDIIHLLQSIFEKFDNIKINK
                     RRGDKILLEKINNIPLSFQKYYPLFELLGEYTGWEFKKEKWNLEEFKIIKLEKKIENS
                     LTGKNFIEIIEKNPISWAITANKRMEYTLGNFG"
     gene            complement(6880..7725)
                     /locus_tag="PLW95_06630"
     CDS             complement(6880..7725)
                     /locus_tag="PLW95_06630"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011959181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOV22334.1"
                     /translation="MDERYKILYNKGMIEKLINKVVCMDCVEGMKLLPDKSIDLVVTS
                     PPYDNIRNYHGFKLDLHSVGMEIYRVLKEGGIAVMVIQDQTKNYSKTLTSFKTIIDWC
                     ENIGFKLFECLIYKKYGAEGAWWNKRFRVDHEYMPVFLKGEKPQYFNKAPLKIPSKWG
                     GVTMTGGATRLTNGKTLKSRQIKINKMKCRGTIWEYLTCGDGDKLKHQHPATFPDKLP
                     VDFIECFCPENGIVLDPFMGSGTTAIASIKLGRKFIGFEISKEYCELAEKRIEMYSIP
                     LFIEK"
     gene            complement(7752..8408)
                     /locus_tag="PLW95_06635"
     CDS             complement(7752..8408)
                     /locus_tag="PLW95_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOV22335.1"
                     /translation="MGIKENLNEIFRKLPGGVELIAATKSRSVDEILEAIECGIKIIG
                     ENYVQEAEEKFKVIGKKVKWHMIGHLQKNKVKKAIEIFDMIETLDNFSLAKEIDKHAK
                     RKEIIYPVLLEINSGREIQKSGVLPEDIEKVVEEISTLENVRIKGLMTMGPLVEKPED
                     MRQYFRLTKELFDKLKEKCFPNVEMKYLSMGMTDTWEIAIQEGANMVRIGTGIFGPRK
                     "
     gene            8455..10122
                     /locus_tag="PLW95_06640"
     CDS             8455..10122
                     /locus_tag="PLW95_06640"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HOV22336.1"
                     /translation="MFSIGLDFGTNSVRCVIVDWINGQVSGEAISNYISGIDGVIIDE
                     KNPHFARQNPSDWLICLEKVIKEAIKKSKKENSFSIDKIIGIGVDTTGSTPLPVDENL
                     TPLCFYPEFQNNPNAMAYLWKDHTSINESEEITQLAKKIRPEYLKKIGGVYSSEWFFS
                     KLLHLARVDKNVFEKMGTFIECSDFIPAVLSGIKKPEEVKRSICAAGHKAMFNEKWGG
                     LPDNEFLEKLDISFKGLRKKLYENTYPAGKIAGYLCKDWAIKTGLKEGIPISVGGLDA
                     HIGAVGAGIKEKILVKVIGTSGCDMMIYPKNKYLEDIPGICGIVPDSIVPGYYGIEAG
                     QPAVGDIFYWFVKKFMPEFTENSYYYFTKKAEKIKPGESGLLCLDWQNGNRTILVDEK
                     LTGLILGFTLNTKPEEVFRCLIEGTGFGEKVIIEQLKKYCGEVNEIIATGGISEKNLL
                     LMQIYSDILGKEIKLPVQQQTCAVGASIFGAIAGGYFKNVNEAQEKICKFKDFVYKPE
                     NKNKKIYDRIYVCYKQLYDAFGTKNYKGTLYNVMKDLLKIKEETTGS"
     gene            10156..10485
                     /gene="metG"
                     /locus_tag="PLW95_06645"
     CDS             10156..10485
                     /gene="metG"
                     /locus_tag="PLW95_06645"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HOV22337.1"
                     /translation="METIKFDDFKKIDLRVGKILEVEEIEGADKLYLLTVDIGDETRK
                     LVAGIKKWYKREELVGKTIVVAVNLEPKVIRGIESKGMLLATLFDTQLSVLTTDKDDV
                     PAGSKIT"
     gene            complement(10511..11368)
                     /locus_tag="PLW95_06650"
     CDS             complement(10511..11368)
                     /locus_tag="PLW95_06650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOV22338.1"
                     /translation="MKVIPSVLKERKKDTHKGNFGHLFIIGGSTGLTGAVCLSSISAL
                     RSGCGLVTIGIPSCLNKIIEVKLTEVMSLPLPNDNGFISEKGIDVAYKFIKEKSDGVV
                     IGPGISLNSGTKKFVMGLLPLIDKKLLIDADALKIISENLSIFEKLKVKPVITPHPGE
                     MKFLTGKEIDEIQRNREKIALKFAKENNVVVVLKGYRTIVSDGKEIYVNLTGNPGMAT
                     GGSGDVLSGIIGSFLVQGMDEFESAKFGVFVHGLAGDFACKEKGEISLIPSDIVEKLP
                     YTFKYLKEK"
     gene            complement(11371..11619)
                     /locus_tag="PLW95_06655"
     CDS             complement(11371..11619)
                     /locus_tag="PLW95_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013933203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOV22339.1"
                     /translation="MGRKKQGNGRDSNPKFRGTKIFAGQVVKGGNIIVRQKGTKIMPG
                     KGVGIGKDFTLFSLIDGVVEFSQKKGKKVVNVVPFSDK"
BASE COUNT         4239 a         1855 c         1727 g         3931 t
ORIGIN      
        1 gttggacctg ttacaagcac aataccacct gaatatttta acaaattctc ccatcttttt
       61 aaatcatatt caagaaaacc caattcttcc aatcccttta acattgatgt tttatcaaga
      121 attctcaaaa caacactttc tccataaatt ccaggtaaag atgaaacacg aaaatcaatt
      181 ggtcttccac ttatatcaag cataattcta ccatcctgcg gaagccgttt ttcagccagg
      241 tccatttttg ccattaactt aactcttgct ataactgctt tctcaatcct ttttggtgga
      301 gagacagtct catataaaac cccatcaatt ctaaacctaa ctctaaattt cttttcaagt
      361 ggctcaaaat gaatatcact tgctcttctt ctcaatgctt caacaaaaac aagatttact
      421 attttaacta ctggcgcctg taatgcaagg gactcttcat catcccctaa ttcttctgct
      481 ggtaaatcca ggtctattgt tgcactactt gcttcctgaa tcatttttgt taagtcctcc
      541 atttctcctt caccataata cttttcaaga agttggtcca tctcttcttt aaaagttacc
      601 ataatttcta catcttttat accctcaata tttatcagcc ctttaaagta atcaccagca
      661 agaatattta tagggtcatc tgtaacaaga acaacaactc cattttctct tttttcccat
      721 ggaaccactc tatgactctt ggcaattttc ccgggtaata gagaaagaac ttcatggggt
      781 atttctcttg cggataattc tctttgaact attcctaatt gaaaataaaa agaaggagca
      841 acctctccaa acggtaacat tcccctattt tgaaaaattt cctttaatgg ctgacctgtt
      901 ttactttgtt cttccagtgc aagctctatt tcttttctac cccaaagacc tacattttga
      961 ataatattta ttacttcttt ttttgttggc attgtataac tataatatca ctttaaaaat
     1021 attttgtcaa caacaatttt ttctttcttt ttttgctatc ttgatttgaa aataatattt
     1081 atccttatca aatcctcacc taaaaattta tttatatttc catccttcat caagcataat
     1141 aagaaaattt tgaacaggaa tatagtttgc tattgaattt ccagaaccca aagcatacct
     1201 ttcaggcata cattggtcaa gaactcttct tacatatttt cttaaatttt cttcattata
     1261 agtacataat ttattaacat caatcccacc caaaatacca attttttcat catataactc
     1321 tttaaattcc catacaggaa tgatttcatc ctgaaaagaa tgaaaagcat ctatctttac
     1381 atcatatata aaatcattca taatttctaa aacatttcca caacaatgaa gaaaatacat
     1441 tttccctttt tcatgcgcaa tttgagaaaa ttttttatga aatggaatta ctaattttct
     1501 tatgtagaac ggtgaaagaa atgttgatgt tttaaaaccc aagtcatccc cttgaaaaaa
     1561 tccttcaaca ttatcaattt caacaatatt ttcataaaaa gataccatca attgtccaac
     1621 tttattaaaa accgcctcaa ctaattcata attttcatat attaaataac tcatattctc
     1681 aaaaccaaga attttctcac ttgatatttc aaaaacacca gaagaagggc aaaccaccat
     1741 tttcattcct tctggtaaat ttttacttgt aatttcataa gttttataat caatttcatt
     1801 tatttttggc catgggtatt tttcaaactc atcccaattt gaaattacac ctttattttc
     1861 ttctacccat cccctctgcc ctcttgaaag aactgcagta tcctctccta ccctcccttt
     1921 cccctcaaaa tcaagccccc ctgatattct cgcataatca taccctaatt gataccagaa
     1981 ataaatttcc tgtttggtcc aatcatcaat attttcataa gaaaaatcaa tccattttct
     2041 cccaaaatat ttttcactaa tatatttttt aatctcataa tcaaaaagaa gttcggcaaa
     2101 atgcaccctt ttcgcttttt ctttcccttc aagaactttt ttgaatgtct caaaatcggc
     2161 ttttttcttt tctgatataa atttgaattc cacattttta attgtaatat ttccttgaaa
     2221 taaatcaata aataaaatat aatttttgta tggaaaatta taaattattt tcgccatctg
     2281 attatagata caaagtagac caacttattg attatcttac agaagaaagt tttcttaatt
     2341 ataaaattaa agttgaaatt gcacttctcc aaacactttc agattttaat ctctgtccag
     2401 aagatgttgt aaaagaagta gaaaaggtag cccggaatat aaaggcagaa gaaatatatg
     2461 aggaagaaaa aaggataaaa catgatataa gggcaattgt taatgttttg aagaaaaatg
     2521 tatctgagaa agcaaaacca tttattcatt tttgtgctac ttcttatgat attgttgaac
     2581 cagcaagatg tcttatgtta aaagaagcaa cagaaaaagt aattattcca gatatgattg
     2641 agcttgaaga tattttcata aaacaggcaa ggaaagaaaa gaaaacaatt caaattggaa
     2701 gaacacatgg acaacatgca gaaccaatta cttttggatt ttttctttct gtttatattg
     2761 aaagatgggg agaaagaatt ttaaaagtaa ataacgcaaa agaaaaccta aaaggaaaat
     2821 tttcaggtcc agttggttgt tataatggac tttcactttt tataaaagat gtagaaaaat
     2881 ttgaaagtac ttttcttgaa aaactttctc ttaaaccatc aagaatttca actcaaattg
     2941 ttccacctga accaatggtt gattttgtcc attctataat ttcttctttt tcagtccttg
     3001 caaatctttc agatgatatg agaaacttac aaagaacaga aataggagaa gttggagagc
     3061 catttgagga aaatcaggtt ggttcttcta caatgccaca aaaaagaaat ccaataaatt
     3121 ttgaaaatgt aaagagtgca tggaaaacat ttatgccaag aattataact tcttattcag
     3181 accagatttc agaacatcaa agggacttaa caaattcttt atcacaaaga tatattccag
     3241 aattgcttgt tatgtttgat tcatctatca gaagaataaa aagtataatg gagaaaattg
     3301 ttgttgataa aaataatttg gtgaaaaatt ttaatatgag taaagacaaa attatagcag
     3361 aaccaatgca aattttactt tctttttatg gacatcctga tgcacatgaa aaaataaggc
     3421 aactttcaat gaaatcatat caaacaggta ttcccttaac agaaattgtt tttcaggatg
     3481 aagaaattaa aaaatatatt gaaaaattta cacctcaaca aagaaaagta atagaaaacc
     3541 cagagaatta tacaggaatt gcaagtcaga aagtgaacaa aatatgcaat ttctgggaaa
     3601 aaaaattaaa aaggatataa aatgggaagt gttaaggact tagaaattat taaaaaagca
     3661 gaaaaagata aaatgggaat tggaagattc catttttcag atagatactc tgtttttgat
     3721 tggggagaaa tgcctgataa tattgatgga aaaggtgcct ctttatgtat tgtaagtgct
     3781 ttcttttttg aaaagattga aagaaaaggc attaaaacac attatagagg ggttgtggat
     3841 aaaaatggaa atttaagaaa gtttggggaa attgaaagac ctgtttctac aatggaaatt
     3901 tctcttgtcc gagttttaaa accaacattt tcatcaggta aatatgatta ttcaatttat
     3961 aaaaaggaaa aaggtaattt tctcattcct cttgaaatta tttatagaaa tgggattcct
     4021 tctggttcaa gtatattaaa acggcttaaa aatggtgaat taaatttgtc agatattgga
     4081 cttgaccatt ttccagtaga aggagaaaaa ttggaaaaac caatatttga tatcagcaca
     4141 aaattagaga tgaaagatag atatatttcc ttaaaagaag gtaaagaaat tgcatgcctt
     4201 tctgatgagg aagtaagtca aatgcaagaa gttctttttg taataaatgc aacaataagt
     4261 gaagaagtca aaaaaatagg gattgaaaat gaagatggaa aaatagaact tggatttacc
     4321 cctgaaagag aatttattgt tgttgatgtt ataggaactc ctgatgaatg cagatttaca
     4381 tacaatggta taaatttaag taaggaaatt gcaagacaat attatagaaa aacaaaatgg
     4441 tatgaagaag tagaaagagc aaaaaatgag gcaaaagata ttggaattga tgattggaaa
     4501 tcactttgta aattaaatcc aaaaaaatta cctacaaaat tgaaaaaaac aattgaaaat
     4561 atttataaat catttgctaa tgaacttacc ggtaaaaaat tctttgccag tcctccttta
     4621 aaagaaatta tacaagaata ctcctcatat ttgccctaat tctggaacta tgtttcagaa
     4681 aacggtgtgt atttgaaaaa atccgaattg cttttgttct tttgcggcaa aatacagtta
     4741 aataataata aaaagagagg gaatttattg agatgaaaaa acaacaaaat ttgaataaaa
     4801 aagtgttacc gatgtgccca actcatacac accacaaaaa agagagaaag tgtgtttatt
     4861 gtgcaataag aaaaatttaa aaatatcagt aattttttaa ataaatttat caattaacaa
     4921 ggaataaaaa attatgaaaa aagacattaa gcaaatcgtt aagaaatatt acggaaaaat
     4981 tgcgactaaa aataccggct gttgtagcag tggttgcggt tgtgaaaata cagcaaccaa
     5041 tgaacaaatt gcagaaagca taggttattc caatcaagaa ataaaatcag cgtctgacgc
     5101 aaatctggga ctcggctgtg gcaatccaac aggaatggga caaattagag agggagatgt
     5161 tgttttagat ctaggttcgg gagcgggatt tgattgtttt ttagcagcca aaaaagttgg
     5221 catatcagga cgtgttattg gcgtagaaat atctgaggaa atggtagaaa gagcaagatc
     5281 taacgccaac aaatacggat ataaaaatgt agagtttaga ttgggcgata ttgaagaatt
     5341 gccggttgag gataattcca ttaatataat aatcagtaat tgcgttatca atcttgcgcc
     5401 ggataagtca aaagttttta atgaggcacg ccgagtcctc aaaaaagggg ggaaaatgta
     5461 tatttcagat attgtattgc tgaaaaattt atcaaaagaa caaaaaggag acaatgaatt
     5521 aatagcgggg tgtgtgggcg gtgctttatt aaaaaatgac tacttgaaaa ttattaaaaa
     5581 agcaggactc aaaataaaag ttttatccga agataaagat ataagcaaaa gacattatca
     5641 aggaataccg cttgagagtt taaaaattga aattcaaaaa taataaagaa aaacccgctt
     5701 ctggcgttct gccttcggcg gaacaaaaac aaaaaattaa actttgcgcc taaaatcggc
     5761 tggcgagccc tcgcaaaggg cgagccaaac ctttcctttt ccaactggcg gagagggagg
     5821 gatttgaacc cccgtccacc cttttcgggc agaagccgat ttcgaatcgg ccgctttcat
     5881 ccactcagcc acctctcctg ttggtaaaaa ttattatttt ataattcaat aatttgttat
     5941 aattatacta tgaattatct taattatgga attattgaga aagaattccc atggattttt
     6001 gaaaaacaac aacaggcaat tataagtcct gatgtagatg ggatattatg cggtttactt
     6061 atgtcaaatt acttagaatg ggaaattgtt ggcttttacg atgggaaaat gctctgtcag
     6121 acaaaaaata taagtatcaa agattgtatt tttcttgata ttgaaatttt tagaaaggaa
     6181 ataagaagtt gtggtcatca tatgctttta tacaacaaga acgaagttcc tgataattgg
     6241 ggaaatttca ataagtcaat caatccaaat aatttaagaa attttgatgc tcttcataat
     6301 tttcaaaaga aatacccatt agcgacaatt cattttcttt tgtgctgttt aaaagaaata
     6361 ttaaataaag aaattaagtt tttaaacacc acaactgttc ctcttttata tgttgatgga
     6421 acttttaaaa atttacttaa ttaccctgaa aattgtaatg aatggcttat gtttcttaaa
     6481 gcgaaaaagc aagatagtcc aatttaccca atttttgaga tattttcaag acagaaactt
     6541 aaagatatta tccatttatt acagagtatt tttgaaaagt ttgataacat aaaaatcaat
     6601 aaaagaagag gagataaaat tttattagag aaaattaata atatcccttt atctttccaa
     6661 aaatattatc cactttttga actattaggt gaatatactg gatgggaatt taaaaaagaa
     6721 aagtggaatt tagaggagtt taaaataatt aaacttgaga aaaagataga aaacagctta
     6781 acgggtaaaa attttataga gattatagaa aaaaacccaa tctcatgggc aattacagca
     6841 aacaaaagaa tggaatacac attaggaaat tttggataat tatttttcaa taaataaagg
     6901 aatagaatac atttctattc ttttttcggc taattcacaa tattcctttg aaatttcaaa
     6961 tccaataaat ttcctaccta actttattga tgctattgct gttgttccac tacccataaa
     7021 tgggtctaac acaatgccat tttcaggaca aaaacattct ataaaatcaa caggtaattt
     7081 atcaggaaaa gttgctggat gttgatgttt taatttatcg ccatctccac aggttaaata
     7141 ttcccagata gttcctctac acttcatttt atttattttt atttgtcttg atttaagtgt
     7201 ttttccattt gtcagtcgtg ttgctcctcc tgtcatagta actccacccc atttagatgg
     7261 aatttttaat ggcgctttat taaaatattg tggtttctcc ccttttaaaa aaacaggcat
     7321 atattcgtgg tcaactctaa atcttttgtt ccaccacgct ccttctgcac catatttttt
     7381 gtatattaaa cattcaaata acttaaaacc aatattttca caccagtcaa taattgtttt
     7441 aaaagaagta agtgttttac tataattttt tgtctggtct tgaattacca ttacagcaat
     7501 acccccttct ttaagaactc tgtaaatctc cattccaaca ctatgaaggt ctaatttaaa
     7561 tccgtgataa ttccttatat tatcgtaagg tggagaagta actacaaggt caatactttt
     7621 atctggaagc aatttcattc cttctacaca atccatacat acaactttat ttataagttt
     7681 ttctatcata cctttattat ataaaatttt gtatctttca tccactcagc cacctctcct
     7741 gttggtaaaa attatttcct tggaccaaaa atcccagtcc caattcttac catatttgct
     7801 ccttcttgga ttgctatttc ccatgtatct gtcattccca ttgataaata cttcatttct
     7861 acatttggga aacacttttc ttttaattta tcaaataact cttttgtcaa cctgaaatac
     7921 tgtctcatat cttctggttt ttcaacaagt ggtcccatag tcatcaatcc ttttattctt
     7981 acattttcaa gagtagagat ttcttcaaca actttttcaa tatcttctgg taagacacca
     8041 cttttttgta tttctcttcc actatttatt tccaacaaaa ctggataaat tatttctttt
     8101 ctttttgcgt gtttatctat ttcttttgca agagaaaaat tgtcaagtgt ttcaatcata
     8161 tcaaatatct caattgcttt ttttactttg tttttttgta aatgccctat catatgccat
     8221 ttaacttttt tccctataac tttaaacttt tcttctgctt cctgaacata attttctcct
     8281 attattttta ttccacactc tattgcctca agtatttcat caacacttct tgattttgtt
     8341 gcagcaataa gttcaacacc gccaggtaat tttctaaaaa tttcatttaa gttttcttta
     8401 attcccattg aattctcctt tcttttatta tacaattttt gtaaggagat tataatgttt
     8461 tcaattggtc ttgattttgg aacaaattct gtaagatgtg taattgtgga ttggataaat
     8521 ggacaggttt caggagaagc aatttcaaat tatatttcag gcattgatgg tgtaataatt
     8581 gatgagaaaa atccacattt tgcaaggcag aatccttctg attggttaat ttgtcttgaa
     8641 aaagttatta aagaagcaat aaagaaaagc aaaaaagaaa atagtttttc cattgataaa
     8701 attataggaa ttggtgtaga tacaacagga agtactcctt tacctgttga tgaaaattta
     8761 actccccttt gtttttatcc tgaatttcaa aacaatccaa atgcaatggc atacctttgg
     8821 aaagaccata catcaattaa tgaaagtgaa gaaataacac aacttgcaaa aaaaattagg
     8881 cccgaatatc taaagaaaat aggaggtgtc tattcctctg aatggttttt ttcaaaatta
     8941 ctccatctgg caagagttga taaaaatgtt tttgaaaaaa tggggacttt tattgaatgc
     9001 tcagatttta tacctgctgt tctctctggt ataaaaaaac cagaagaagt aaaaaggtct
     9061 atttgtgcag caggacataa agcgatgttt aatgaaaagt ggggaggact tccagataat
     9121 gaatttcttg aaaaacttga tatttctttt aaagggttaa gaaaaaaact atatgaaaat
     9181 acctatccag cagggaaaat tgctggatat ttatgtaagg actgggctat aaaaacagga
     9241 ctgaaagaag ggatacctat tagtgtagga ggacttgatg cccacattgg agcagtagga
     9301 gcaggcataa aagaaaaaat acttgttaaa gttataggga cttctggatg tgatatgatg
     9361 atatacccta aaaataaata tttagaagat attcctggta tttgtggaat tgttcctgat
     9421 tctattgttc caggatatta tggaattgag gcaggacaac ccgctgttgg agatattttt
     9481 tattggtttg taaaaaagtt tatgcctgaa tttactgaaa attcttatta ctactttaca
     9541 aaaaaagcag agaaaataaa gccgggagaa agtggacttt tatgtcttga ctggcaaaat
     9601 gggaatagaa caattcttgt tgatgaaaaa ttaacagggc ttattcttgg gttcacttta
     9661 aatacaaaac cagaagaagt tttcagatgt ttgattgaag gaactggttt tggagagaaa
     9721 gtaataattg aacaactcaa gaaatattgt ggtgaagtaa acgaaataat tgcaacagga
     9781 ggtatttctg aaaaaaatct tcttttaatg cagatatact ctgatatttt gggaaaagaa
     9841 ataaaattac ctgttcaaca acaaacatgt gcagtaggag caagcatttt tggggcaatt
     9901 gcaggaggat attttaaaaa tgtaaatgaa gcacaggaaa aaatttgtaa atttaaggac
     9961 tttgtctata aacccgaaaa caaaaacaaa aaaatttatg atagaattta tgtgtgttat
    10021 aaacaacttt atgatgcatt tggaacaaaa aattataaag gaactcttta taatgtaatg
    10081 aaggacttac ttaaaataaa agaagaaaca actggaagtt aatttatata atttttataa
    10141 aaaatggagg agataatgga aacaataaaa tttgatgatt ttaaaaaaat tgatttaagg
    10201 gtaggaaaaa ttttagaagt tgaagaaata gaaggagcag acaaacttta tcttttaact
    10261 gttgatattg gggatgaaac aagaaaatta gttgcaggaa taaaaaagtg gtacaagaga
    10321 gaagaactgg ttggaaaaac aatagttgtt gctgttaatt tagaaccaaa agttataaga
    10381 gggattgaga gtaaaggtat gcttcttgca acattatttg atactcaact ttctgttttg
    10441 acaactgata aagatgatgt ccctgctggc agtaaaatta cttaattttt ataaataaac
    10501 tggtaaaaac tcatttttct tttaagtatt tgaaggtata aggtaatttt tcaactatat
    10561 cagaaggtat taatgaaatt tcaccttttt ccttacaggc aaaatctccg gcaagtccat
    10621 gaacaaaaac cccaaatttt gcactttcaa actcatccat tccctgaaca agaaaactgc
    10681 caataatacc tgaaagaaca tctccacttc cacctgttgc cattcctgga tttcctgtta
    10741 aattcacata tatttctttt ccatcactta caatagttct ataccccttt aaaacaacaa
    10801 ctacattatt ttcctttgca aatttcaagg caattttttc tctatttctt tgtatttcat
    10861 caatttcttt gccagttaaa aatttcatct ccccgggatg gggtgtaata actggtttta
    10921 cttttaattt ttcaaaaata cttaaatttt ctgaaataat ttttaaagca tctgcatcaa
    10981 taagaagttt tttatcaatt aatggtaata atcccataac aaactttttt gttccactat
    11041 ttaaagaaat cccgggacct ataacaacac catcactttt ttcttttatg aatttatatg
    11101 caacatcaat acctttttct gaaataaaac cattatcatt aggtaaaggt aaactcataa
    11161 cttctgtaag ttttacctca attattttat ttaaacagga aggaatacca atagttacaa
    11221 gtccacaccc actccttaaa gcagaaatag aggaaaggca aactgctcct gttaagccag
    11281 tactacctcc aattataaaa agatgtccaa aatttccttt atgagtatct ttttttcttt
    11341 ctttaaggac tgaagggata actttcatta ttatttatct gaaaaaggca caacatttac
    11401 tactttttta cccttttttt gtgaaaattc aacaactcca tcaattaaac tgaaaagagt
    11461 aaagtccttc ccaattccca cacccttgcc aggcataatt tttgttcctt tttgtctaac
    11521 aataatattg ccacccttaa caacctgccc agcaaaaatc ttggttcctc taaattttgg
    11581 atttgaatct cttccattac cttgtttttt tcttcccata gtgctttaat tatacttttt
    11641 aaaaatttta ttgtcaacag gtcggcattc ctcaaaaata atctctttga aagagagtag
    11701 tttcctcata aataaatttg acaaagataa aggccatcct gtatatatga at
//
LOCUS       DAOOVW010000048        11707 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_3587_length_11707_cov_8.351785, whole genome shotgun sequence.
ACCESSION   DAOOVW010000048 DAOOVW010000000
VERSION     DAOOVW010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11707
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3587_length_11707_cov_8.351785"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            276..2243
                     /locus_tag="PLW95_06660"
     CDS             276..2243
                     /locus_tag="PLW95_06660"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOV22340.1"
                     /translation="MRNTIYLNDGWKLCWRSFRDEKDIDKLTTGSTDDWISATVPGDV
                     HIDLLKNGIGPDFYYGLNADLWRWVEEKDWWYRTEINTPNIQHNEVVHIIFEGVDTFS
                     TIFINGEKIITNKNMFTPIEIDITQYCKKYQNIKIAVKISSPIFSVDVNKDQKIVDSN
                     LPRMFARKAQYNYGWDIAPRLISIGIWRPVRIETYNCGRIKDFWITIKKLDKKKSILE
                     CECEIEYFKQIENGEVKIEIFEGAIDGQGNKIIETSKKISSKNFPLKISINLKNPKIW
                     WPNGFGEQNLYSYIVSLKKDGKIIDEKRGNFGIKKIELIQEKLSKSERSFYFKINNKK
                     IFIKGLNWTPLDALPGSIENEKYEKVIKLVKNINANMLRVWGGGIYEPDIFYDLCDKY
                     GIMIWQDFMFACGRYPENKEFLNEVKDEATYVIKRLRKHVCISLWCGGNENDTFRYWE
                     DGHNYIKHKCRNVLKKVWEKLNTQTPFIPDSPFSPSENDPNDDKEGDVHLWAHGKSYK
                     DNFYIKKRPKFVSEIGHISVPDKKTLLSFIPDEKLWPVFNEYWYYHSSDTLRVGWKYR
                     INSLFNSIKANNLPQPENIDQLIEITQNLQAEAYKFWVEFYSSIPDCGGILLWNVCDC
                     WPQISDSIITYSLTPKKAYYKVKEIFKKAEY"
     gene            complement(2484..2636)
                     /locus_tag="PLW95_06665"
     CDS             complement(2484..2636)
                     /locus_tag="PLW95_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22341.1"
                     /translation="MNKIKERLKESGWLDEKPENSFTSFLDILQGVELGILEIEKVKK
                     NAECIN"
     gene            complement(3782..5875)
                     /locus_tag="PLW95_06670"
     CDS             complement(3782..5875)
                     /locus_tag="PLW95_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22342.1"
                     /translation="MDKILFKNYGGSYQIVIEKGEDLLNVLKLDEGLWAATSVPIEIL
                     NCDKKFLEYLDIDNNKRIRTDEVRNAISYILSLIKNPSIVDGNDTLLLSDINDQTETG
                     KLLIKTAEIILTNSGIEEKEKINLSQVRNSQEIMAKSILNGDGIITPDSSEKEEISSL
                     IKIIIEKIGWKIDVNGEKGIDKEIVDEFFKECSEYLNWYEKGIIPDDKETTDIMIFGK
                     ETPYLHNIFTQIEEKIEQYFLLSNFLKFDEKTEELLKLKEDDFKNIDIENRDILIEKL
                     KKLPLAPPNKNLILDFDGIINILYKDKIKNFKENIVNKIFPNSNKLTLENYEKIKKMF
                     SNYKDWIENKKGEKVEQIGIENIKKYIEGNYKEEIEKLIEKDLSVLPFIQSIKDLEKL
                     ILYKKYIIEFLNNFVSFSNVYNPDITSLYEAGVLVIDGRKINLNILVKDINAHKKVSS
                     MSNTFIMYLNISGKENNQSQFQIASPLTSGDMGRIRLGKRGIFTDRSGNEWDAEIVDI
                     IKNPVGLLEGIKAPFIKIGENIKNQIEKITETRQKKVEQTLTSPSASSSIRDIMIGGG
                     VAFAALGTCFAYITRVFSQIKFTHILLAIVVICALIFIPSFIITIFRLRGRDLSILFE
                     ASECALNVKMRLGTKLGKILTYIPNFPENSEKLKVDVLSGFTKIKSRKRLKVLIFILI
                     LLLIFIIFYYKYLLP"
     gene            complement(5891..7723)
                     /locus_tag="PLW95_06675"
     CDS             complement(5891..7723)
                     /locus_tag="PLW95_06675"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOV22343.1"
                     /translation="MKEKIIEPTENSKCNVFPVAIKNVKLSGFLGKRMEINRKVSIPS
                     LYKNFEKYGTVNNFRIVSGEKSGEITKRLATDSDLYKWIEGVSFDLQNTYNKENEKLL
                     EYLIKIIGKSQEKSGYLNTNAYLTTKKFDNLKFSHELYCGGHLIQSAIAHFRSSGKDN
                     FLNIAIKWADYICKEFGKGKLEKTDGHPEVEMAFVELYRTTKEKKYLEMAKFFLEVEN
                     PEYWGLPNIPFIKFKELEGHAVRMLYLSCGATDYYSETGDKKYKETLLKLWNDLSERK
                     IYITGGVGSRYDGETFGYPYELPNLRSYCESCASIASMMWQYRMFLLFPESKYFDIFE
                     TTLYNGFLSGISFDGEKYFYVNPLASRGNYERKQWYGTTCCPPNIQRMIASLPGYFYS
                     INEKGIYINLYGEGISEISLFSGEKVKIIQKTKYPFEGKVEIDIALDKEKQFSIFLRI
                     PSWSKKTKIKDGKDEFSPNPGYYEIKKIWKGENKIIINFDISPSFYISHPEIESTKNC
                     IAIKKGPIVYCLESIDNPKISLFSSKIKKQKLNEKFEKGFFDWVPIIYGEIMNLETTK
                     LPIYENEKFYPELIYKKERFKAIPYFLWANRGKSKMVVWIEKAY"
     gene            complement(7735..8727)
                     /locus_tag="PLW95_06680"
     CDS             complement(7735..8727)
                     /locus_tag="PLW95_06680"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HOV22344.1"
                     /translation="MEKIAILVVPEQLEKNFGKEEIEKLKNYGEVKVWNGNENFKEFL
                     KGATIIITSWDSPEITSSIFSFAPDIKFIMHSAGSIKSIVEREVMEKGVRVSSCSNVL
                     GKFVAITALGYILTSVKKIFWWNDFIKNTGKWRENEKLMEYTDEIVGPDIGIISLSNV
                     GKNLLRFLRIFGEQIYVYDPYWSEERIKNLGGKKVNTLLEIAEKCEIIALCAPLTEET
                     KGMIGKDFFQKMKDGSVFINTARGAIIDENALIEELKKERIFACLDVTCNEPLDKTSP
                     LRKLKNVVISPHIAGLVKNGLKELGKFSVEEVVRFIRGEKLENEIIYEKLDITA"
     gene            complement(8740..10038)
                     /locus_tag="PLW95_06685"
     CDS             complement(8740..10038)
                     /locus_tag="PLW95_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012966665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF561 domain-containing protein"
                     /protein_id="HOV22345.1"
                     /translation="MDKFPVIQVALDFTELKRAIKVAQEVVKGGIDWIEVGTPLIKST
                     GVEAIRQIKKEFPNKKIIADMKIMDAGRLESEIAFKSGADIVVVLGNASDQTIKECVS
                     AGENYGGKVMVDLMEESANLKRAKEVEKLGVDYISVHTPIDEQMTGNISFDIVKDIAK
                     NIKIPVAVAGGLNSENISDAIKSGASILIVGGAITKSENVENEVKKLKEAVKKLKKIP
                     SLYYKRVKKGNIREVLLKVSTDNISDALHRGGWLKDISPVLGRQFKMAGKAITVRTFP
                     GDWAKPVEAIDISEKGDVIVIDAGGVSPAVWGELATSSAKVKGVSGVVIYGYIRDVEE
                     IIEMNFPSFAVGITPQAGEPKGFGEINVPIKIGGQVVKPGDWIVGDIDGVVVIPEEKV
                     VEITNRAMDVFERENRIRKEIKDGSTLSSVAELLRWEKTK"
     gene            complement(10060..10464)
                     /locus_tag="PLW95_06690"
     CDS             complement(10060..10464)
                     /locus_tag="PLW95_06690"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV22346.1"
                     /translation="MGNKERVAVLIVEKEFAGLEAISDFVQRMCSQEKIGFKKTWELM
                     LSIDEICSTLITCSYNDGQFIRVIWVSSENKVHVKIEENGSPFNPLKNFDEEDAIYGL
                     GPQIIETMVDEAKYERENNFNCILITKKIRKK"
     gene            complement(10454..11614)
                     /locus_tag="PLW95_06695"
     CDS             complement(10454..11614)
                     /locus_tag="PLW95_06695"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOV22347.1"
                     /translation="MKICHIITRLVIGGAQENTIFTVEGLKKKGYNVDLVSGKTKGPE
                     GSLENEVRNKNINLIIIPQLIRNLNPFYDIIAFIKIFKVLKKNKYDIIHTHSGKAGVL
                     GRMAGKLSNRKTTVIHTIHGPSFYPNQKKFAYLFYKITEKIAGKFTDHFICVGEIMKE
                     RYLKNGIGKENQYSVIYSGFPITPYIEVQNKREYLRKKLRIDKDEKVIGMIGRLFPLK
                     GQEYLLSAFSMLVNNFSNIKLIFVGDGILKEKLKNIAKEKGIEKKVIFTGLVPPEKIP
                     EYVSVIDIVVHTSLREGLPKAVAQALAGGKPVVSFNVDGAKEIVIDGQTGFLVKSKDI
                     NKLAEKIKILLENPEIAEKMGKVGREKILSMFSVDKMVDEIEKIYLNYKNGK"
BASE COUNT         3845 a         1861 c         1406 g         4595 t
ORIGIN      
        1 taaatcatag tgaaggggaa tatgtaaata aagaaggaaa tcacataaat ggacttgtcc
       61 aaaggctccc ctcgggcgta gacccaacgg ccgagcctgt ggatttgata tttttgaaaa
      121 tatgttttac aatattttta ggtaagtaaa aataattaaa tttagtggct aaaattaaag
      181 ataatccaaa aataagtact ttttggtatt ttaatatatg ttaattgtag ttaaatagaa
      241 aatattaatt aaacaattat tcgggataaa aaaatatgag aaacacaatt tatttaaatg
      301 atggatggaa attatgttgg aggtctttta gagatgaaaa agatattgat aaacttacaa
      361 cgggctctac tgatgattgg atttctgcga ctgttcctgg ggatgttcat attgatttat
      421 taaaaaacgg tattggtcct gatttttatt atggattaaa tgcagattta tggagatggg
      481 ttgaagaaaa agattggtgg tatagaactg aaataaatac tccaaatatt caacataatg
      541 aagttgttca tataattttt gaaggagtag atacattctc aacaattttc ataaatggtg
      601 agaaaataat tacaaataaa aatatgttca caccaataga aattgatata acccaatatt
      661 gtaaaaaata tcaaaatata aaaattgcag ttaaaatatc ttctcctatt ttttcagttg
      721 atgtaaataa agaccaaaaa attgttgatt caaatttacc aagaatgttt gcaagaaaag
      781 cacaatataa ttatggatgg gatattgctc caaggttaat tagtattgga atatggagac
      841 ctgtcagaat agaaacatat aattgtggtc gaataaagga cttctggata acaataaaaa
      901 aattagataa aaaaaaatct attttagaat gtgaatgtga aatagaatat tttaagcaaa
      961 tagaaaatgg ggaagttaaa atagaaatat ttgaaggagc aattgatgga caaggaaata
     1021 aaataataga aacttcaaaa aaaatatctt ctaaaaattt tcctttaaaa atatcaataa
     1081 atctaaagaa tcccaaaata tggtggccaa atggttttgg agaacagaat ctttattcat
     1141 atattgttag tttaaaaaaa gatggaaaaa ttattgatga aaaacgagga aattttggga
     1201 ttaaaaaaat tgagttaata caggagaagt tatctaaaag tgagaggtct ttttatttta
     1261 aaataaataa caaaaaaatt tttataaaag ggcttaactg gacaccatta gatgctttac
     1321 ctgggagtat agaaaatgaa aaatatgaaa aagtaataaa acttgttaaa aatataaatg
     1381 caaatatgtt aagagtttgg ggtggaggaa tatacgaacc agatatcttt tatgatttat
     1441 gtgataaata tggaattatg atatggcagg actttatgtt tgcatgcggt agatatcctg
     1501 aaaacaaaga atttttaaat gaagttaaag atgaagcaac ttatgtaata aaaagattaa
     1561 gaaaacatgt atgtatttct ttatggtgtg gtggaaatga aaatgataca tttagatatt
     1621 gggaagatgg acacaattat ataaaacata aatgtagaaa tgtgttaaaa aaagtatggg
     1681 aaaaactaaa tactcaaact ccttttattc ctgatagccc attttcaccg tcagaaaatg
     1741 accccaatga tgataaagaa ggtgatgttc atctatgggc tcatgggaaa agttataaag
     1801 ataattttta tataaagaag agaccaaagt ttgtaagtga aattggacat atatctgttc
     1861 ctgataaaaa aacactttta tcctttattc ctgatgagaa attatggcct gtttttaatg
     1921 aatattggta ttatcattca tcagatacat taagagttgg atggaaatat agaattaact
     1981 ctcttttcaa ttcaataaaa gcaaataatt taccacaacc tgaaaatatt gaccaattaa
     2041 tagaaataac acaaaatctc caggcagaag catataaatt ctgggttgaa ttttactcat
     2101 caatacctga ttgtggtgga atacttttat ggaatgtttg tgattgctgg cctcaaatat
     2161 cagatagtat tattacatat tcacttactc ccaaaaaagc atattataaa gtaaaagaaa
     2221 tttttaaaaa agctgaatat tagtttttcc gaccagatac taataccata ttttaatgat
     2281 agaataaaat tattctaaaa ataagatatg aggcggcggc acagaaatgt tttaattcac
     2341 tttacccctt caaaacaaga aaaccatata ggatagggga ttaagcaatt tattgtagta
     2401 tagcaatctt tctttttatc ttttatacat cagtaaaata ctcttcgtct atctcaaaaa
     2461 ttcctaatat tctttttgtc tcatcagtta atacattcag catttttttt tactttctct
     2521 atttcaagta tacctaattc tacaccctgt aaaatgtcta aaaatgatgt aaaactattc
     2581 tctggtttct catctaacca accactttcc tttaatcttt cttttatttt attcacagac
     2641 cagctgtgcc agacctggtc tggaggagga ttaacatatt ttttcagtag atttattatg
     2701 tctcaattta ctttttggat agtaaaaaca ttaattttaa tttttattta aaaaaatatt
     2761 gtgtgaacta acctttttta aaaaagaaag gtattttaaa taggatggta gatactcttt
     2821 ttgacattcg gatattcaag tggaggagga aagaaaataa aaaatataat agatgagagg
     2881 gtagggtagt aaattttaaa atctttttgt tctttcttta aaatttttag caaataaggg
     2941 ggaatcatct tccatattgc cttgagagga acaatagtat actgctggat aaataccttt
     3001 tacctttttt ctaatcattt tatcaaaggt taaaatcaaa gcattatact caatccctaa
     3061 ggtagccaat atcaaatcat aggttttaag ctctcttata gtagaagata tatagagaag
     3121 tagcaggtat atccgtgatc attatttctt ttctatgtaa taatttccac aatttttttt
     3181 ccactctgga ttttgaaatt ccttttttca ataatgtctt tgttgtttct aataacataa
     3241 ctgaaggaat aattattttg gttttatcca aaaagtgcga gagtaattca actattttcc
     3301 aagcatcttc aaaatatctg ttccatctaa aactgcaatt atgaaactgg tatcttctat
     3361 tataaaggtc atttatacct catttcatat aaaatttcat tggcaggttt agaattttta
     3421 ggctgctctt tctttgttat tactaattct tttttcattt ttcttatcat cttttctatt
     3481 tttttcctca tcatttttct tttttttcat ttttatacct ccaaataaac tttacaatat
     3541 tctaatttta gtgttaattt aaaaaatttt tggtatccaa atagtatcca tatgatgtcc
     3601 aaaatcatac aaattcatac aaaatggtat aaaaaaaatt catataaatc attgataatc
     3661 aatggaatta caacaatatc aagaattttc agaaagtggt taattagcat tacaagtgcg
     3721 ccgctctgcc gctgagctaa ggtggcatta tttaaactat tatattaaat tatttttttc
     3781 tttatggcaa taaatattta taataaaaaa tgataaaaat tagaagaaga attaaaataa
     3841 aaataaggac ttttaatctt tttcttgatt ttatttttgt aaaacctgac aaaacatcta
     3901 cttttaattt ttctgaattt tctgggaaat ttggaatata agttaaaatt ttccctaatt
     3961 ttgttcctaa tcgcattttt acatttaagg cacattcgct tgcttcaaat aatatgctta
     4021 aatctcttcc tcttaaccta aaaatagtta ttataaaact tggaataaaa attaaagcac
     4081 atataacaac aattgcaaga agtatatgtg taaatttaat ctgtgaaaaa acccttgtaa
     4141 tataagcaaa acatgtgcca agtgcagcaa aagcaactcc acctccaatc attatgtccc
     4201 ttattgaaga agatgcagaa ggagaagtaa gtgtttgttc tacttttttt tgtcttgttt
     4261 ctgtaatttt ttcaatctga ttttttatat tttcacctat ttttataaaa ggtgctttta
     4321 ttccttcaag caagcccaca ggatttttta ttatatccac aatttctgca tcccactcat
     4381 tcccgcttct atcagtaaaa attcctcttt taccaagccg aattcttccc atatctccag
     4441 atgttaatgg agatgcaatt tgaaattgag attgattgtt ttctttccct gagatgttta
     4501 aatacataat aaatgtatta ctcattgaag atactttttt atgggcattt atatctttta
     4561 caagaatgtt taaatttatt tttcttccat caattacaag tactcctgct tcatacaaag
     4621 aagtaatatc aggattatat acatttgaaa aactgacaaa attatttaaa aattcaatta
     4681 tatatttttt ataaagaatt aacttttcaa ggtctttaat actctggata aaaggtaaaa
     4741 cagataggtc tttctctata agtttttcta tttcttcttt gtaatttccc tcaatatact
     4801 ttttaatatt ttctattcca atttgttcta ctttttctcc ttttttgttt tcaatccagt
     4861 ccttataatt tgaaaacatc ttctttattt tttcataatt ctctaatgtt aatttatttg
     4921 agtttgggaa aattttattt actatatttt ctttgaaatt ttttatttta tctttatata
     4981 ggatatttat tattccatca aaatccagaa ttaaattttt atttggtgga gcaagaggta
     5041 atttttttaa tttttcaatc agtatgtctc tattttcaat atcaatgttt ttaaaatcat
     5101 cttctttcaa ttttaaaagt tcttctgttt tttcatcaaa ttttaagaaa ttagaaagta
     5161 gaaaatactg ctctattttt tcctctatct gggtaaatat attatgaaga taaggggttt
     5221 cttttccaaa tatcattatg tctgttgttt ctttatcatc agggattatt cctttttcat
     5281 accagtttag gtattcacta cattctttaa aaaactcatc tacaatttct ttatcaatcc
     5341 ctttttctcc attaacatct attttccaac ctattttttc tattataatt tttataagag
     5401 atgaaatctc ttctttttca gatgagtccg gggtaataat tccatcacca tttaaaatac
     5461 tttttgccat aatttcctgc gagtttctta cctgacttaa atttattttt tctttttctt
     5521 caattcctga atttgtaagt attatttctg ctgtttttat taataatttt cctgtttctg
     5581 tctggtcatt tatatctgaa agaagtagag tatcatttcc atcaacaata gaagggtttt
     5641 ttatgagaga aagtatataa gatatggcat ttcttacttc atctgttctt attcttttgt
     5701 tattatctat atcaagatat tcaagaaatt ttttatcaca atttagtatc tcaattggaa
     5761 cacttgttgc tgcccaaaga ccttcatcta atttcagaac atttaataag tcctctcctt
     5821 tttcaattac tatttgataa ctgccaccat agttcttaaa aagaatttta tccataatta
     5881 tcctctaatt tcaatatgct ttttctatcc atacaaccat ttttgatttt cctctatttg
     5941 cccagagaaa ataaggaatt gctttaaatc tttctttttt ataaataagt tctggataga
     6001 acttttcatt ttcataaatt ggcaatttgg tggtttctaa attcattatt tctccataaa
     6061 ttattggtac ccaatcaaaa aatccttttt caaatttttc atttaatttt tgttttttta
     6121 ttttagaaga aaaaagagat attttaggat tatcaatact ttctaaacaa taaacaatag
     6181 gacctttttt aatagcaata caattttttg tgctttctat ttcaggatga gaaatataaa
     6241 aagaaggaga aatatcaaaa tttataataa ttttattttc tcctttccat atttttttta
     6301 tttcataata tcctggatta ggagaaaatt catctttacc atcttttatt tttgtctttt
     6361 tactccacga tgggattctt aggaaaattg aaaattgttt ttctttatct aatgcaatat
     6421 ctatttcaac ttttccctca aaaggatatt ttgttttctg aattatcttt actttttcac
     6481 ctgaaaaaag agaaatttca cttattcctt ctccataaag atttatatat attccttttt
     6541 catttataga atagaaatat ccaggtagag aagcaatcat cctttgaata tttggtggac
     6601 aacaagtagt accataccat tgttttctct cgtaatttcc ccttgaagca agtggattta
     6661 cataaaaata tttttctcca tcaaatgaaa tacctgaaag aaaaccgtta taaagtgttg
     6721 tttcaaaaat atcaaaatat ttgctttcag gaaataaaag gaacatcctg tattgccaca
     6781 tcattgaagc aatagaagca caactttcgc aataacttct taaatttggt agttcataag
     6841 gatacccaaa ggtttctcca tcatatcttg aaccaactcc acctgttata taaatttttc
     6901 tttcagataa gtcattccat aattttaaaa gtgtttcttt gtatttttta tctcctgttt
     6961 cagaataata atcagttgct ccacaagata aataaagcat tcttactgca tgaccttcaa
     7021 gttctttaaa ttttataaaa ggtatatttg gtaatcccca atattctgga ttttctactt
     7081 caagaaaaaa tttcgccatt tctaagtact ttttttcttt tgtagttcta taaagttcaa
     7141 caaatgccat ttcaacttca ggatgtccat ctgttttttc taatttccct tttccaaatt
     7201 ctttacaaat atagtcagcc cattttatag caatatttaa gaagttatct ttccctgaac
     7261 ttctaaaatg ggcaattgct gattgtatta aatgcccgcc acaatacaat tcatgagaaa
     7321 attttaaatt atcaaatttt tttgttgtta aatacgcatt tgtgttcaag tatcctgatt
     7381 tttcctggga ttttccaata atttttatta aatattctaa taatttttca ttttccttgt
     7441 tataagtatt ttgtaaatca aaagaaacac cttcaatcca tttataaagg tctgaatcag
     7501 ttgcaagtcg ttttgttatt tcaccacttt tttcaccaga aacaattcgg aaattattta
     7561 ctgtaccgta tttttcaaaa tttttgtata aagaaggaat acttactttt ctgttaatct
     7621 ccattctttt tccaaggaaa ccagataatt ttacattttt aattgcaaca ggaaaaacat
     7681 tacattttga attttctgtt ggttcaataa ttttttcttt catttcacca cctattaagc
     7741 agtaatatca agtttttcat aaattatttc attttcaagt ttttctcctc ttataaatct
     7801 tacaacttcc tcaacagaaa attttccaag ttcttttaac ccatttttta ccagaccggc
     7861 aatatgagga gagataacaa cattttttaa ttttcttaaa ggactggttt tatcaagagg
     7921 ttcattgcat gttacatcaa ggcaggcaaa aattcgctct tttttaagtt cttcaataag
     7981 ggcattttca tcaattattg ctcctcttgc tgtatttata aaaacacttc catctttcat
     8041 tttttgaaaa aagtcctttc ctatcatacc ctttgtttcc tcagttaatg gggcacaaag
     8101 agcaattatt tcacactttt ctgctatttc cagtaaagta ttaacttttt tccctcctaa
     8161 atttttaatt ctctcttctg accaatacgg gtcataaaca taaatttgtt cgccaaatat
     8221 ccttaaaaat cgtaataagt ttttcccaac atttgataaa cttataattc ctatatcagg
     8281 accaacaatt tcatctgtgt attccatcaa tttctcattt tctctccact ttcctgtatt
     8341 ttttataaaa tcgttccacc agaaaatttt ttttacagaa gtaagaatat atccaagtgc
     8401 tgtaattgca acaaattttc ctaaaacatt tgaacaactg ctaactctta ctcctttttc
     8461 cataacttct ctttcaacaa ttgatttaat tgaacctgct gaatgcataa taaatttaat
     8521 gtcaggagca aaactgaaaa ttgaagaagt tatttcagga gaatcccaac ttgttataat
     8581 tattgttgct ccttttaaaa attccttaaa attttcatta ccattccata cttttacttc
     8641 tccataattt tttagttttt caatttcttc ttttccaaaa ttcttttcaa gttgttcagg
     8701 aacaactaaa attgcaattt tttccatttt tctcttatgt tattttgttt tttcccatct
     8761 taaaagttct gccacactac ttaaagtaga accatcttta atttcttttc ttatcctatt
     8821 ttctctttca aaaacatcca tagccctatt tgttatttct acaacctttt cttctggtat
     8881 tacaacaacc ccatctatat ctccaacaat ccaatcacct ggtttaacaa cctgccctcc
     8941 tatctttatt ggaacattta tttctccaaa cccttttggt tctcctgcct gtggagtaat
     9001 accaacagca aatgaaggaa aattcatttc tattatttct tctacatctc ttatatatcc
     9061 ataaataaca actcctgaaa cgccctttac ttttgcacta cttgttgcta attctcccca
     9121 cacagcagga ctaactccac ctgcatcaat aacaataaca tccccttttt cagaaatatc
     9181 tattgcctca actggttttg cccaatcacc tggaaatgtt cttactgtta ttgcttttcc
     9241 tgccatttta aattgcctac caagaacagg actgatatct tttaaccagc cacctctatg
     9301 taaagcatct gaaatattat cagttgaaac tttaagtaga acttctctta tatttccttt
     9361 ttttactctt ttataatata aagatgggat tttttttaat tttttcactg cttctttcaa
     9421 tttttttact tcattttcta cattttctga cttagttatc gctcctccaa ctataaggat
     9481 agatgctcca ctttttattg catctgatat attttcagaa tttagtccac ctgcaacagc
     9541 aacagggatt tttatatttt ttgcaatatc tttaacaata tcaaaagaga tattccctgt
     9601 catttgttca tcaattggcg tgtgaactga tatataatca actcctaatt tttcaacttc
     9661 ttttgctctt tttaaatttg ctgattcttc cattaaatca accattactt ttcccccata
     9721 attttcacct gcacttacac actcctttat tgtctggtca gaggcattcc caagaactac
     9781 aacaatatct gcacctgatt taaatgcaat ttcactttca agtcgtccag catccattat
     9841 tttcatatct gcaattattt ttttattggg aaattccttt tttatttgtc ttattgcttc
     9901 tactcctgtt gatttaatta agggagtacc aacttctatc caatcaatgc ctcctttaac
     9961 aacttcttgt gcaactttta ttgctctttt cagttcagta aaatcaagtg caacctgaat
    10021 tactggaaat ttatccattt tttatctcct gtgttcctac tattttttcc ttattttttt
    10081 tgtaattaat atacaattga aattattttc tctttcatat tttgcttcat ctaccattgt
    10141 ttcaataatc tggggaccaa ggccataaat agcatcttct tcgtcaaaat ttttaagtgg
    10201 gttaaatgga gaaccatttt cttcaatttt aacatgaact ttattttcac ttgataccca
    10261 tattactctt atgaactgac catcattata tgagcaggtt attaaagtag agcatatttc
    10321 atcaatagat aacattaatt cccatgtttt tttaaacccg attttttcct gtgaacacat
    10381 tctctgaaca aaatcagaaa ttgcttcaag tccagcaaat tctttttcta caattaaaac
    10441 agcaactctt tctttatttc ccatttttat aatttaaata aattttttct atttcgtcaa
    10501 ccattttatc aactgaaaac atgctcaaaa ttttttctct cccaactttt cccatttttt
    10561 ctgctatttc agggttttct aaaagaattt ttattttctc agccagttta tttatatctt
    10621 tactttttac taaaaagcca gtctgtccat ctattacaat ttcttttgct ccatccacat
    10681 taaaagatac aactggtttt ccacctgcaa gggcctgtgc tactgctttt gggagtccct
    10741 ctcttaaact tgtatgtaca acaatatcta ttactgaaac atattctgga attttttcag
    10801 gtgggacaag acctgtaaat attacttttt tttcaatccc tttttctttt gcaatgtttt
    10861 ttaatttttc tttcaagatg ccatctccaa caaaaataag ttttatattt gagaaattgt
    10921 taacgagcat agaaaaagca gaaagtaaat attcctgtcc ttttaatggg aataatcttc
    10981 ctatcattcc aataactttt tcatctttat caattctcaa tttttttctt aaatattctc
    11041 ttttgttttg aacttcaata tatggtgtaa taggaaaacc actgtaaatg acagaatatt
    11101 gattttcttt tcctatgcca tttttaagat acctttcttt cataatttct ccaacacata
    11161 taaaatggtc agtaaatttt cctgctattt tttcagttat tttgtaaaaa agataagcaa
    11221 attttttttg gtttggataa aaagagggcc catgaattgt atgaataaca gttgtttttc
    11281 tatttgataa ttttcctgcc attcttccaa gaactcctgc ttttccacta tgggtatgaa
    11341 ttatatcata tttatttttt ttaaggactt taaaaatttt aatgaaagca attatatcat
    11401 aaaatgggtt taagtttctt ataagttgag gaattattat taaatttata tttttatttc
    11461 tgacttcatt ttctaaggaa ccttctggtc ccttagtttt tccacttaca aggtcaacat
    11521 tatatccttt ttttttaagc ccttctactg taaaaattgt attttcctgc gctccgccaa
    11581 taaccaatct tgtaataata tgacatattt tcatatcagt gcagtaattc tcattaatat
    11641 ttagttagca ggtatattaa ggagttttat agcattttct cttgcaactt tattgaaaac
    11701 aatttct
//
LOCUS       DAOOVW010000049        11311 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_3726_length_11311_cov_9.179549, whole genome shotgun sequence.
ACCESSION   DAOOVW010000049 DAOOVW010000000
VERSION     DAOOVW010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11311
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3726_length_11311_cov_9.179549"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..371
                     /locus_tag="PLW95_06700"
     CDS             111..371
                     /locus_tag="PLW95_06700"
                     /inference="COORDINATES: protein motif:HMM:NF024064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HOV22348.1"
                     /translation="MKTDIATNLYFPKELYADMKKISKKLGISIAEYVRQVVQKDIES
                     RIKEIDWENDPLWDIIGIGETKESDISINHDHYLYGGQKKEK"
     gene            371..781
                     /locus_tag="PLW95_06705"
     CDS             371..781
                     /locus_tag="PLW95_06705"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOV22349.1"
                     /translation="MRSIFADTSALFSLIDRTDRYHKKAIELSKKTRKENLEIVISDH
                     ILSETITLVRNKMGFPQASLMGIKLFDSRITTLIIVDENILKMAWDIFSKYTDKDFSF
                     VDCISFAVMKQTGIDTAFTFDHHFVEIGFRILGR"
     gene            1153..1308
                     /locus_tag="PLW95_06710"
     CDS             1153..1308
                     /locus_tag="PLW95_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22350.1"
                     /translation="MFYTVSLRKAYEPALQGRDKISRRDLIKGDTEKSTLTLLYKGRE
                     RKEQSGF"
     gene            complement(1717..2877)
                     /gene="yaaA"
                     /locus_tag="PLW95_06715"
     CDS             complement(1717..2877)
                     /gene="yaaA"
                     /locus_tag="PLW95_06715"
                     /inference="COORDINATES: protein motif:HMM:NF015820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HOV22351.1"
                     /translation="MNKNILFIITCSNGKIEGGITNYNYKSSICNYISSECISYLMEG
                     RQFNLTHIKNGGFTRQKKILKDLPYNKELKNSFDFGGKEEAKFLPAYERYCGRIYKEI
                     DKETWKNRRHQVLIFSGLYGWVLPEEFIQRYSLHLRDSQLIIDIWKGEIATFILESFV
                     QRNNIDVLVDCTGEDLYREIVDWEYFEARGYDVFHIYGEQNAGPSVLPAIGYFLQNKG
                     LTGEDSELLNLMNKIKYFQTTYEKIWFIKGKKELEELKLPREEESYELQEKVNQIVTS
                     IERPSEFDEIESIHKVKIIFNYKVLSQIIELPKEIYGKFLQNLKLYIKNPEHAGLKTE
                     KIQKDGETFYRFRINDFYRVHVDPYIKDPVDKNKKILNIRAVGGAKVESISH"
     gene            complement(3015..3788)
                     /locus_tag="PLW95_06720"
     CDS             complement(3015..3788)
                     /locus_tag="PLW95_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22352.1"
                     /translation="MKKKIPKRDDKFQRYIKQIDDTWGEEVYEFTILKKYKVDFWKYQ
                     IYHTQRWLSYSIAMAYTNQHKNVHNKISRMAIKSYHLSSGLVLLPACWDKLAQLLRCF
                     LGIKFSKENLQGRSAEEKERKLTNFQKIMDYLKKGNTCIENKIYRTLLPYWNENKEES
                     KRRKRIYDLANEVKHKIHPEYLGIPVKDYIEIKEEELNQGRFSFGSKAIKEDVLYEHI
                     KLLKKANNSFVKCVVKIDQIIDFEQFYKKPKNCKKIVSL"
     gene            4037..5014
                     /locus_tag="PLW95_06725"
     CDS             4037..5014
                     /locus_tag="PLW95_06725"
                     /inference="COORDINATES: protein motif:HMM:NF014610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HOV22353.1"
                     /translation="MMKKALGLVSGGLDSLIAISLIKEQNFDVIGVFILTPFISKFGE
                     ETIEILKKQSSELNFKLCVVPIDIDYIEIIKNPVFGYGKNLNPCIDCHIYMLKKAKEI
                     MEKENASFVFTGEILGQRGKSQTLQALKIIEEKSSLKGKLLRPLTALNLPETEIEKNG
                     IVDRNKLLGIKGKERKMQLTLAEIKNLKFFSSPAGGCLLTDSQFCKRLSDIFKYNVDA
                     EINDYYLLQVGHHFRISPETKLVISRNRKESGKILELFSNKDIIILSSDVNKDIIGII
                     IGGFSEICLKIFASYISKSPVSIIIEEKGKKEEKIVQPEEKFLYKAYLI"
     gene            5492..6208
                     /locus_tag="PLW95_06730"
     CDS             5492..6208
                     /locus_tag="PLW95_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HOV22354.1"
                     /translation="MGRERFYRNIMKIDNLKKFEVKVKETDLLIFAEKDIKKEIEEEV
                     KKQRRIIETYIKKHPEFYLSFSPVDVENDEEIIKLMSISSKLTKTGPMASVAGCISEV
                     IGKKFLSISKEIFIENGGDIFMNLKNEVKIGIYAGSSPFSMKIGIKIKKDGPYGIATS
                     SGTVGHSFSYGDADAVTIISSSATFADGAATYFGNLIKGKIDKEVIEKEIENFPFVDG
                     ILIIRGKELFVWGNMELVKF"
     gene            6178..6738
                     /locus_tag="PLW95_06735"
     CDS             6178..6738
                     /locus_tag="PLW95_06735"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOV22355.1"
                     /translation="MGKYGIGKILKKRSTFGWLFFVVIAIFGKGNLKQILIGFFIVVI
                     GEFFRTFASGIIKKNQEVAKDGPYKWCRHPLYFGSFLISTGLLISSFNIVPLYNIIPL
                     IYFLIFFPLFYIPTMKNEEKFLKEKFGEEYTVYSKNVPAFFPLFKKTKKENFSFTQVK
                     KNSEYYNWIAIFLVYLVLVLKLLIIY"
     gene            6912..7682
                     /gene="rpsB"
                     /locus_tag="PLW95_06740"
     CDS             6912..7682
                     /gene="rpsB"
                     /locus_tag="PLW95_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOV22356.1"
                     /translation="MMEIEIQNLLEAGVYFGHPSRQFDPRIKPYLYGRRQGIYIIDIE
                     KTIEKLKEAGEFLKKVAANGGKILLVGTKKQAQTIIKEIGEKCEMPYVNYRWIGGTLT
                     NFNEIRKRINRLKEIEDYEKSGKINLYTKKEAQLLMKEKEDLLKKFGGIRDMESFPDA
                     IVVVDVRREINAIKEAKKANIPIVGIVDTNGNPEIVDYPIPGNDDGFKSINLLLSVIS
                     EYILEGKKEGAYVVEEKNKESKEDNKGEKSTKEEKNES"
     gene            7672..8259
                     /gene="tsf"
                     /locus_tag="PLW95_06745"
     CDS             7672..8259
                     /gene="tsf"
                     /locus_tag="PLW95_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451211.1"
                     /note="EF-Ts; functions during elongation stage of protein
                     translation; forms a dimer; associates with EF-Tu-GDP
                     complex and promotes exchange of GDP to GTP resulting in
                     regeneration of the active form of EF-Tu; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HOV22357.1"
                     /translation="MKADIDKIKELREKTSLSVMECKNALEKTGGDLEKALEYLKKRG
                     FEMSEKKESRETKEGLIGSYVHINGKVGTLVEVNCESDFVSRNEEFKELVKNICLQIT
                     ATGPKYIDRNSVPDEEKNKIKDIVKEEFKDKKEEIIEKIIEGKLNDFYKENVLLDQIY
                     VKDENITVNEYIKSKIAKLGENIKVKRFSRFEIGE"
     gene            8281..8553
                     /locus_tag="PLW95_06750"
     CDS             8281..8553
                     /locus_tag="PLW95_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22358.1"
                     /translation="MEFEIGYMLALVIVGLSFIGFFLAMMINEINKKKGVVIFILSLI
                     LFGFGIYYYYVVGQWQKMKGGPSVNKFNYYLNIYSPSPSILPYENN"
     gene            8540..10414
                     /locus_tag="PLW95_06755"
     CDS             8540..10414
                     /locus_tag="PLW95_06755"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HOV22359.1"
                     /translation="MKTIKIFKLWFFVFFSFIFYVFPQNNFSYDEESSVYLLDYAKVE
                     VDSNFKSKTLIHQKIKIIGQKGKRFAELRIPYDADRQKVKIISALTITPEGKILKATK
                     ENIKIVTPAELTEYTALYPGVKTICVNLPGVEIGSTVEYKYEIYTSRPLINGHFYDGF
                     YFQSVEPFLISKYELKIPKKIKIFIYEEGIKLKEKKESSAYTTYIWENNNANPIIEEP
                     FMPPLYEIVPRVYITTFNSWEEIGKWYYNLSKDCSKPDENIIKKVNELIEDKKTEEEK
                     ISAIYNFVCQKIRYVGVELGPYGFKPHDAKDVFHLKYGDCKDKANLMKTMLEIAGIKS
                     YITLVNTDGKIEKDIPFPGQFNHAIVAIENNNNFLFLDPTSEVYRFPQIPPSDQNKYV
                     LISKPGLPLEKTPLFAPEENYIKRKINTTLYENGDMLSNVEIETNGLYDAIFRSSFIY
                     LKDIERQRALSSELNSILPGTNLISFEIEGIENLEKNVIEKYSFKTNSFATVIAEHKI
                     IFTPGIIDTLKDTSIVSLEKRNFPLRFGYLFKKEEIVVYTLPENFKVEILPSPIEIDN
                     NFATFKYEIKENDGKIYYRRIFEIKKDEITIDEYNDFRNFYRTVSKIDRLPVILTKIE
                     "
     gene            complement(10513..10791)
                     /locus_tag="PLW95_06760"
     CDS             complement(10513..10791)
                     /locus_tag="PLW95_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOV22360.1"
                     /translation="MVKKEIIEKIAERTGLNRHLVRRVVDEFLSVVSEAFKEKERVEL
                     RRFGVFYFKKRKRKIGRNLKTGEEIIIPEGEKLIFKPSLSKKNRGDEE"
     gene            complement(11041..11126)
                     /locus_tag="PLW95_06765"
     tRNA            complement(11041..11126)
                     /locus_tag="PLW95_06765"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11090..11092),aa:Leu,seq:caa)
BASE COUNT         4107 a         1607 c         1804 g         3793 t
ORIGIN      
        1 ggttatagtt cccttttatt ccaaaacaaa acatcccttc tttacaaata aattttaaag
       61 aagtataata gtattaatat tagttggtaa taatagtagg aggaaaaaaa atgaaaacag
      121 atatagcaac aaacttatat tttccaaaag aactctatgc tgatatgaaa aaaatatcta
      181 aaaaattagg catatcaatt gctgaatatg taaggcaggt tgtccagaaa gatattgaat
      241 ctcgtattaa agaaatagat tgggaaaatg accctctttg ggatattata ggaattggcg
      301 aaacaaaaga atctgacata tctattaatc acgaccacta tctttatggt ggacaaaaaa
      361 aggaaaaata atgagaagta tttttgcaga tacatcagcc ctcttctcct tgatagacag
      421 aactgaccgc tatcataaga aagcaataga gttatctaaa aaaactcgca aagagaattt
      481 agaaatagtt atttcagacc acattttatc tgaaacaatt actcttgtca gaaataaaat
      541 gggttttcca caagcatctc ttatgggtat aaaactattt gattccagaa ttacaacttt
      601 aattatcgta gatgaaaata tattgaaaat ggcatgggat attttttcaa aatatactga
      661 taaagatttc agttttgttg attgtatttc ttttgctgtt atgaaacaaa caggaataga
      721 cactgctttt acttttgacc atcattttgt agaaattgga tttaggatat taggaagatg
      781 aagaaaaaag ggaaaaacaa gaagggacta tcttaaaaat actttacaaa actacctgtt
      841 tattcaaaaa tttagaacac ttcgtaaaaa gatgatgaaa aaaagtttcc gtatttatac
      901 tgactgaaaa tcccgcaaat tttaataact atacagttaa taccaatttg acaacggaca
      961 tagagtaaat attttcccct cacctatcct ctcctctcag gggagaggct cgcccgtagg
     1021 gctacgcccg aggggaagat atcccttacc cacaagttcc cactttgaaa aaccgactaa
     1081 ggaagcaaag gggagcaaag cagagttggt gcgttatcgt cttccagata gcacaacggg
     1141 gatggagggg gattgttcta cacagtcagt ttaaggaaag catacgaacc agccctacaa
     1201 ggtagagata agataagcag gcgggattta ataaaaggag atacagagaa atccacccta
     1261 accctccttt acaaaggaag ggagagaaag gaacagagtg gattttgaaa aatataccac
     1321 aaactttgtt gcattatttg aaaagtgata aagtaagaaa aatgtagtat ttttcaattt
     1381 tataaaagaa aataggagca aagggaatct ctttatccaa gaggattgta gaagatgtgg
     1441 gtaagggata ggaggggaag ggtgatgggg gtagttcctt ctacccaatg gggagagggt
     1501 atgggtgagg gggctgattt ttattacact ttttgatgta aaaatagtat gaatatctta
     1561 gtgtccaaaa atatgggaat ttaggaagga aagtttccgt atttatactg ataaggaata
     1621 tttttgaata ggtcttataa aatatacata atttaatccc tactgttata ttttttaaga
     1681 tggcttatcc atacaaagag ttctaaaaat tcctttttaa tgagaaatac tttcaacttt
     1741 ggcaccacct actgctctaa tattaagtat ctttttattt ttatccactg ggtctttaat
     1801 ataagggtct acatgaactc ggtaaaaatc attaattcta aaacggtaaa aggtctctcc
     1861 atctttctgt attttttctg ttttaagacc tgcgtgttca ggatttttta tatataattt
     1921 caggttttgg aggaattttc cataaatttc ttttggtaat tctataatct gagagagaac
     1981 tttataatta aagataattt ttactttatg tattgattca atttcgtcaa attctgatgg
     2041 tctttctatg gaagtgacta tttgattcac cttctcctga agttcatagg actcttcttc
     2101 tcgaggcaac tttaattctt ctaattcttt ttttccctta atgaaccata ttttttcata
     2161 agttgtttga aagtacttaa ttttgttcat taaatttagt aattcggaat cttccccagt
     2221 taaacctttg ttttgtaaaa aataacctat tgctggtaaa actgatggac ctgcattttg
     2281 ctctccatat atgtgaaata catcatatcc tcttgcctca aaatactccc agtcaacaat
     2341 ttctctatat aagtcctctc ctgtacaatc aactaataca tctatattgt tcctttgcac
     2401 aaatgattca agaataaaag ttgctatttc tcctttccat atatctatta tcaattgaga
     2461 gtcccttaaa tggagtgaat aacgttgtat aaattcttct ggtaaaaccc atccataaag
     2521 tccagaaaaa ataagaacct gatgtctcct atttttccat gtctctttgt caatttcttt
     2581 ataaatcctt ccacaatatc tttcatacgc tggcaaaaat ttcgcttctt cttttcctcc
     2641 aaaatcaaaa ctatttttca attccttatt atatggtaaa tccttcaaaa tttttttctg
     2701 tcgtgtaaaa ccgccgtttt tgatgtgagt aaggttaaat tgtcgtcctt ccattaaata
     2761 agaaatgcat tctgaagata tataattaca gatagaactt ttataattat agttagttat
     2821 tccaccttca atcttaccgt tagaacaggt tattataaaa agaatatttt tattcattta
     2881 tttctccata ctgatatgtt atgatatatt aaaaataaca tcaagtttac tctttaaata
     2941 ttatttcgca tgaatttact tctttggtta tgcccttcca tttaaatcgc tgttttttta
     3001 aaaaagcaat caatttacaa agatacgatt ttcttgcaat ttttgggctt tttataaaat
     3061 tgttcaaaat ctattatttg atcaatttta acaacacatt taacaaaaga attattggct
     3121 ttttttaaaa gttttatatg ctcgtagaga acatcctctt taattgcttt actaccaaat
     3181 gagaatctcc cttgatttaa ttcttcttct tttatctcta tataatcttt tacgggaata
     3241 cccaaatatt caggatgtat tttatgttta acttcatttg ctaaatcata aattctcttt
     3301 cttcttttgc tttcttcctt gttttcattc caataaggca ataaggttct atatatttta
     3361 ttttctatac aagtatttcc cttttttaaa taatccataa ttttctgaaa atttgtaagt
     3421 tttctctcct tttcttcggc cgatctccct tgtaaatttt ctttagaaaa tttaattccc
     3481 aaaaaacatc ttagcaattg tgctaactta tcccaacatg caggtaacaa aacaagtcca
     3541 gaagataaat gatatgattt aattgccatt ctagatattt tattgtgaac atttttgtgt
     3601 tgattggtat atgccatagc tattgagtaa ctaagccatc tttgagtatg ataaatctga
     3661 tatttccaaa aatctacttt atattttttt agtatagtaa attcataaac ttcttctccc
     3721 caagtatcat ctatttgttt tatatatctt tgaaatttat catctctttt aggtatcttc
     3781 tttttcattt gatttccccc tctatccccg ttgtgctatc tggcaaaatc caccccgacc
     3841 ctcctttata aaaggaggga gaacgcacca actctgaggc acaaggtaag caacttcagt
     3901 cggtttaata aaggggagaa ccaaaaagag gagaagtata tgggataaag gggaattaaa
     3961 ggaatattat ttggtggcaa atttgtataa gaaaaaaaat actttttgtt ggttgcatat
     4021 tgaggtaaaa ttgagtatga tgaaaaaagc gctcggactt gtttcaggag gacttgatag
     4081 tttaattgca atttctctta ttaaagaaca gaactttgat gtaataggtg tttttatttt
     4141 aacccccttt atatctaaat ttggtgaaga aacaatagag attttaaaaa aacaatcctc
     4201 cgaattaaat tttaaactct gtgtagttcc aattgatatt gattacatag aaattataaa
     4261 aaatcccgtt tttggatatg gaaaaaatct aaatccatgt attgattgtc atatttatat
     4321 gttaaaaaaa gcaaaggaaa ttatggaaaa agaaaatgct tcttttgttt ttacagggga
     4381 aattttggga caaagaggga aatcacaaac attgcaggca ctaaaaataa tagaagaaaa
     4441 gtcatctcta aaaggaaaat tattaagacc ccttactgct ttgaatttac cagagacaga
     4501 aattgaaaaa aatgggattg ttgatagaaa taaactttta gggataaaag gaaaagagag
     4561 aaaaatgcaa ttaactcttg cagaaataaa aaatttaaaa tttttttctt ctcctgctgg
     4621 tggatgttta ttgactgatt ctcaattctg taaaaggtta tctgatattt ttaaatataa
     4681 tgtggatgca gaaattaatg actactatct tttgcaagtt ggacatcatt ttagaatttc
     4741 tccagaaact aaacttgtaa tttcaagaaa tagaaaagaa agtgggaaaa ttttagaatt
     4801 atttagcaat aaagatataa ttattctttc atcagatgtc aataaggata taattggaat
     4861 aataattggg ggtttttcag aaatttgtct taaaattttt gcttcatata tttcaaagtc
     4921 acctgtttca ataattattg aggaaaaagg taaaaaagaa gaaaaaatag ttcaaccaga
     4981 agaaaagttt ttatataagg catacttaat ttaagaataa ataatggcgg tgtaaagtaa
     5041 aaaggacccc cctcatccga gcattctccc caacggggag aaggacgaaa atcccctctc
     5101 taactcccct ttattaaagg ggagaattaa aaatgggaat gacgcatcaa ctctgttttg
     5161 ctactctttg cttctttagt cggttttaca aagaggggaa atatcccccc tcatcctgca
     5221 ccttctccct aacagggaga aggacgaaaa tcccccttta aatccccctt aatccccgtt
     5281 gtgcctattg aaatacatta cggcaccaac tctgtgtgct aaactttgct acttttatcg
     5341 gtttacaaag ggggaagtat ttgggagaac gcaccaagtc tgaaaaacaa ggtaagcaaa
     5401 tttagtcggt ttatcaaagg gggaatgggg atatttatct taaactctgt acttcttacc
     5461 tggtgaatac ttaaaattta aggaagggaa aatgggaaga gaaaggtttt acagaaatat
     5521 aatgaaaata gataatttaa agaaatttga agtaaaagtt aaagagacag accttcttat
     5581 ttttgcagaa aaagacatta aaaaagaaat tgaagaggag gtaaaaaaac aaagaagaat
     5641 tatagagaca tatataaaaa agcatcctga attttatctt tccttttctc ctgttgatgt
     5701 tgaaaatgat gaagaaataa taaaacttat gtcaatttca tccaaattaa caaaaacagg
     5761 accaatggct tctgttgcag gatgtatctc agaagttatc gggaagaaat ttttatcaat
     5821 ttcaaaagaa atatttattg aaaatggggg ggatattttt atgaatttaa aaaatgaggt
     5881 taaaattggt atttatgcag ggagttcccc tttttcaatg aaaataggta taaaaattaa
     5941 aaaagatggt ccatatggta tagcaacatc aagtggaaca gttggacatt ctttcagtta
     6001 tggggatgca gatgcagtaa ctataatttc aagttctgcc actttcgctg atggagcagc
     6061 aacttacttt ggaaacctta taaaaggtaa aattgataaa gaggtaattg aaaaagaaat
     6121 tgaaaatttt ccatttgttg atggaatttt aattataaga ggaaaggaac tttttgtatg
     6181 gggaaatatg gaattggtaa aattttaaaa aagagaagta cttttggatg gctttttttt
     6241 gttgtaattg ctatttttgg aaaggggaat ttaaaacaga ttttaattgg tttttttata
     6301 gtggtaattg gagagttttt cagaactttt gcctctggaa ttataaagaa aaaccaggaa
     6361 gtagcaaaag atggtcccta taaatggtgc agacatcctt tatattttgg aagtttttta
     6421 atttcaacag ggcttctaat ttcatctttt aatatagttc ctctttataa tataattcct
     6481 cttatttatt ttttaatttt tttcccattg ttttatatac caacgatgaa aaatgaagag
     6541 aaatttttaa aagaaaaatt tggagaagaa tatactgtct attcaaaaaa tgttcctgct
     6601 tttttcccat tgtttaaaaa aacaaaaaaa gaaaattttt ctttcacaca agtcaaaaaa
     6661 aattctgaat attacaactg gattgctatt tttcttgttt atttggtcct tgttttgaaa
     6721 ttattaataa tttattgata ggaaattatt atttcaagtt ctgttataat aaggaactaa
     6781 tataatttat tatttttcaa tatatgcgtt gattttcctt tttttttatg ttaaagtaaa
     6841 taatgttgac attattttgg gaaatttagt aaaataatta gaattaactt taaaaataaa
     6901 aaaggataat aattatggaa attgaaattc agaatttgtt agaagcgggt gtttattttg
     6961 gacatccatc aagacagttt gaccctcgta taaaaccata cttatatggt agaagacagg
     7021 gaatatacat tattgatatt gaaaaaacca ttgaaaaatt aaaagaggca ggggaatttt
     7081 taaaaaaagt cgctgcaaat ggaggaaaaa ttttattggt tgggacaaaa aaacaggcac
     7141 agacaataat aaaggaaatt ggggaaaaat gtgaaatgcc ttatgtaaat tatagatgga
     7201 ttggtggtac tttaacaaat tttaatgaaa taagaaaaag aattaaccgc ctaaaggaaa
     7261 ttgaggacta tgaaaaatca ggaaaaataa atctctacac aaagaaagaa gcacaactac
     7321 taatgaaaga aaaagaggat ttgttaaaaa agtttggtgg tataagagat atggaaagtt
     7381 ttcctgatgc gattgttgtt gttgatgtga gaagggaaat aaatgcaata aaagaagcaa
     7441 aaaaagcaaa tattccaata gttggtattg ttgatacaaa tggcaatccc gaaatagttg
     7501 actatcctat tccaggaaac gacgatggat ttaaatcaat caatttgtta ctctcggtta
     7561 tttctgaata tatattggaa ggtaaaaaag aaggtgcata tgtagttgaa gaaaagaata
     7621 aagaatcaaa agaagataat aagggagaaa aaagtacaaa agaggagaaa aatgaaagct
     7681 gatatagata aaataaagga attaagagaa aaaacttcat taagcgttat ggagtgtaaa
     7741 aatgcccttg aaaaaacagg tggtgatttg gaaaaagcac tggaatattt aaaaaagagg
     7801 ggatttgaaa tgtcagaaaa aaaagaatca agagaaacaa aagaaggact tattggctct
     7861 tatgttcata taaatggaaa agttggtaca ttggtagaag taaattgtga aagtgatttt
     7921 gtcagtagaa atgaagaatt taaagaactc gtaaaaaata tctgcttgca gataacagca
     7981 acaggcccaa aatatattga taggaattct gttcctgatg aagaaaaaaa taaaataaaa
     8041 gatatagtta aagaagaatt taaagataaa aaagaagaaa taatagaaaa aattattgaa
     8101 ggcaaattaa atgattttta taaagaaaat gttttacttg accagattta tgttaaagat
     8161 gaaaatataa ctgtgaacga atatataaaa tcaaaaattg caaaattagg ggaaaatata
     8221 aaagtaaaaa ggttttcaag atttgaaatt ggagaataaa atttaagaaa ggaggagagt
     8281 atggaatttg aaattggata tatgctggca ttagtaattg ttggtctttc ttttattggt
     8341 ttttttcttg caatgatgat taacgagatt aataagaaga aaggagtagt aatttttatt
     8401 ctatcactca tactttttgg atttggaatt tactattatt atgttgtagg ccaatggcaa
     8461 aaaatgaaag gaggtccatc agttaacaaa tttaattatt acttaaatat ttatagtcct
     8521 tctccttcaa tacttcctta tgaaaacaat taaaattttc aagttatggt ttttcgtctt
     8581 ttttagtttt attttttatg tttttcccca aaataatttt tcttatgatg aagaatcttc
     8641 tgtttattta cttgattatg ccaaagttga ggttgattca aactttaaaa gcaaaacatt
     8701 aatacatcaa aaaataaaaa taatcggaca aaagggaaaa agatttgcag agttgagaat
     8761 accctatgat gctgaccgac aaaaagtaaa aataatatct gcacttacaa ttactcctga
     8821 aggaaaaatt cttaaagcga ctaaagaaaa tataaaaatt gttacaccag cagaactaac
     8881 tgaatatact gctctttatc caggagtaaa aacaatttgt gttaatcttc caggagtaga
     8941 aataggcagt acagttgaat ataaatatga aatatataca tccagaccac ttataaatgg
     9001 acatttttat gatggatttt acttccagtc agtagaacca tttctaattt ctaaatacga
     9061 acttaaaatt cccaaaaaaa taaaaatttt tatatatgaa gaagggatta agttaaaaga
     9121 aaaaaaagaa tcttctgctt acacaacata tatctgggaa aataataatg ctaatccaat
     9181 tatagaagaa ccatttatgc ccccacttta tgaaattgtt ccaagggttt atataacaac
     9241 attcaattcc tgggaagaaa ttggcaagtg gtattacaat ctatcaaaag attgttcaaa
     9301 accagatgaa aacattataa aaaaagtaaa tgaattgatt gaggataaaa aaactgaaga
     9361 agaaaaaatt tctgcaatat ataactttgt ctgtcagaaa ataaggtatg ttggagttga
     9421 acttggccca tatggtttta aaccgcatga tgcaaaagat gtctttcact taaaatatgg
     9481 tgattgtaaa gataaagcca atcttatgaa aacaatgctt gaaattgctg gaataaaatc
     9541 atatatcacc cttgtaaata ccgatggaaa aatagaaaaa gatattcctt tcccaggaca
     9601 gttcaatcat gcaatagttg caatagaaaa taataataat tttctttttc ttgaccctac
     9661 atctgaggtt tatcgttttc ctcaaatacc tccatcagac cagaataagt atgttctaat
     9721 ttccaaacca ggattacctc ttgaaaaaac acctcttttt gctcctgaag aaaattacat
     9781 aaaaaggaaa ataaatacaa ccttatatga aaatggagat atgctatcca atgtagaaat
     9841 agaaacaaat ggtttatatg atgctatttt tagaagtagt tttatatatc ttaaagatat
     9901 tgaaagacaa agggcgcttt cttctgaact aaatagtatt ctccctggaa ctaatttaat
     9961 ttcatttgaa attgagggaa ttgaaaattt agaaaaaaat gttatagaaa aatattcatt
    10021 taaaacaaac tcatttgcaa ccgtaatagc agaacataaa ataattttta cccctggaat
    10081 aatagacaca cttaaagata cttctattgt ctcattagaa aaaagaaatt ttccattaag
    10141 atttggttat ctttttaaga aagaagaaat tgtagtttat acacttcctg aaaattttaa
    10201 agttgagatt ttaccttctc ctattgaaat tgataataac tttgctactt ttaaatatga
    10261 aataaaagaa aatgatggga aaatttatta tagaagaatt tttgaaatta aaaaggatga
    10321 aataacgata gatgaataca atgattttag aaatttttat agaaccgtat caaaaattga
    10381 ccgccttcca gttattctta caaaaattga atgattcatt tttactttct ttaaatgggt
    10441 ttttcatttc catcttaaac gaacaagatg aaagaaacat aattgaaaaa attaacaaaa
    10501 gtattaattt tttcattctt cgtctcctct attttttttg cttaatgatg gtttaaaaat
    10561 aagtttttca ccttctggaa taataatttc ctcacctgtt tttaaatttc tgccaatttt
    10621 ccttttcctt ttcttaaaat agaaaacacc aaatcttctt aattctactc tttctttttc
    10681 tttaaatgcc tctgaaacaa ctgataaaaa ttcatcaacc actctcctca caagatgcct
    10741 attcaagcca gttctttctg caattttttc tattatttct tttttgacca ttttttatct
    10801 ctatattctg tggaggactg tcctccacaa tatcacaccc gctgggattg ttcttgttca
    10861 tcacaatccc ttacttcgct cccttcaact tcctcttcaa tgatgtcaat tatgccggag
    10921 gagggagttg gcgccctcgc atcccacaac tgctcgggtc ggccacccgc tgggattgtt
    10981 cttattcatc acaatccctt acttcgctcc cctcaacttc ctcttcaatg atgtcaatta
    11041 tgccggagga gggagttgaa ccctcacgga gtttaagctc caccggattt tgagtccggc
    11101 gcgtctgcca gttccaccac tccggctgta ctcttctttt acagctctac tgacgaccta
    11161 tattcttaca aaatttctct gcttgtcaca gttgtgctac aattttcttc ttttttactt
    11221 ctttttctat aattgctgtt tttttctcgt ttgctcaaat ttttcactct catccgcacc
    11281 agctcggcgt aagcaaatca tagtgaagta t
//
LOCUS       DAOOVW010000050        11153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_3797_length_11153_cov_9.231573, whole genome shotgun sequence.
ACCESSION   DAOOVW010000050 DAOOVW010000000
VERSION     DAOOVW010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11153
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3797_length_11153_cov_9.231573"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="PLW95_06770"
     CDS             <1..598
                     /locus_tag="PLW95_06770"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOV22361.1"
                     /translation="YYKTDWSVIMNKKKLILTVAEKIISEKGYSNTTIEEITKRAKIG
                     KGTFYNYFKNKEELFYLIIKEGLDNLVIEIKDSIEDIDDFFERIKMGIEMYLKYHQKH
                     FYFFKILIQEKPFLKNKKFINFWEDFFDRWDFIKKDLKKQIERNKIVDIEPDDILYSL
                     LGILHGNIHKWHISGRKYCLVEKKDAIFNVFINGIRRK"
     gene            600..1856
                     /locus_tag="PLW95_06775"
     CDS             600..1856
                     /locus_tag="PLW95_06775"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOV22362.1"
                     /translation="MRYFLIMLLCFPCFVFSENFFTLPDAIKVAINNNLEIKISEDKI
                     EQAYYKKEEVKTYFFPKFTSSFAYTYSGDSEKFRTDDNLYNLSLSLSQPIYTGKRIKE
                     TYEISKENLDNAKLEYNVQLQNLIFNVKKGYFSILQANKFLKTSERYKQMLEKHMDDA
                     KKLFEQGLVTNLDILRTEVSVKNAETQIIQAENLIKISKSNFNYILNNPIDNEFEVED
                     VLEIKGDKRDYEYWKTLALENRPEIKSMEKLLSIYNRAINVERSNLYPQVYLFYNYNT
                     DRGIQVSPNSWNPNWNTGIALTLDIWNWGETKDKVKQAESEKKQVEKQLEITKKSIEL
                     EVKNCYLNLLANEKSIEERLKQMETAEENLRVANLLYQEGMATTTEVIEAATSLTEAT
                     NNYSQALYDYHISFAELEKASGKGVE"
     gene            1856..2965
                     /locus_tag="PLW95_06780"
     CDS             1856..2965
                     /locus_tag="PLW95_06780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOV22363.1"
                     /translation="MKKKILLICILILFVIGLGNAIVKNIKKRRGNEVKEEVVKIPVK
                     VMQVKRGDVREVVSASGDIQPWSQVTVFSKVTGQVEKLFVDKGYYVKKGGLIAQVDYR
                     NNALAVKQIENQLSAARINYENLKKDYERMKDLFNQKVISEKKLDDTRTACESAYHEV
                     ESLEAQLELAKIKLGDTRITSPINGVVAEKFIDEGEIITEASMSKSAPIVTIVDIDTV
                     KIVVNVGEKKISKVKKGQSVEVKVDAYPDKLFIGKVYNISPITDPQTRTTEVEISVKN
                     QNYLLKPGMFARTNIIITSHKNVIVIPFDAIVEREGKKCVFVVEGNYAKMKEIDTGLQ
                     EVDKIEVTSGLTENENLIIEGQNTVKDGIEVMLVE"
     gene            3009..6119
                     /locus_tag="PLW95_06785"
     CDS             3009..6119
                     /locus_tag="PLW95_06785"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOV22364.1"
                     /translation="MSIPEIGVKRPVTTAMIFIGILILGGVSLTRLGLDLLPDIEIPS
                     IMVLTTYEGAGPEEVEERITRVAEEQLATVDNLDKIEAFSQEGMSGITLRFNWGTNLD
                     NAVNDIRDKLDLVKPMLPDEADDPIIYKFDVSMMPILVYGVSADQTYPRLKKLLEDNV
                     CIPLESVTGVASAVVRGGLERAILVELDAERLKAYNISIAQVENAIVAANLSTPGGHI
                     KEGKFDYIVRVPEEIKIKEIEDVAIGVYNGIPIKIKDIAEVKDSFKEITQETHVNRRS
                     GMTIMIQKESDANTVNTCRAVFKKLEALKETLPSDVKFSLVSDSSDQIVKSINNLKNS
                     LISGGILVILVIFLFLGNIRSSLIIACAIPISLIITFVFLYMAGYTINIISLSALAIA
                     IGMVVDASIVVHENIHRHLENGEEVINSCIVGGNEVAGSISASVLCMVAIFLPIMFTG
                     GLTAIFFGQLAFVCSAALIASLFNALMLIPMLSSKLLSIPQKEDTNGKKKGILDKITS
                     FAEVWFKKLDNAYGDLLRWCLRNRKKVVFGSATLLFLSLLLVKFIGTEFIPEMDEGRV
                     AFKITLPIGTRYEKTGEITKGVEDIIYKNVPEIQTGFSRWGEGGGGLSSLLGGEEGSN
                     IGNVTLKLIDKEKRNRTPRQIAYDIKDKLNVFPDAEVRYNPSSMSAMMFGGAKPLSVE
                     VRGYDFDQARTLSHKVEEILKNTKGVSDVEISRKEGRPELQVLIDREKASTLGLNVAT
                     ISHMVETSFKGKVSTYYRESGDEYDIEVKLREEDRTKKENLENLFITLPSGKKIHLTE
                     IAEIRETAGPLKIERKGRERMVRVEGEIYGRDLGSVVNEINEKLEKLQLPQGFSFYFG
                     GEYEEQQKSFRQLTFALILGIILVFMIMAAQFESIIDPFVIMFAIPFALIGVVWALFL
                     SGNTISIFSFIGVIMIVGIGLETGIVLISFIKDLRKAGVELHEAVVQAGKLRLRAVLM
                     TTLTTIFGLLPLALSKGEGAEMWVPLGWSLIGGLLVSFLLTLFFVPILYTIYEEKILQ
                     RGKKKA"
     gene            6254..8104
                     /locus_tag="PLW95_06790"
     CDS             6254..8104
                     /locus_tag="PLW95_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22365.1"
                     /translation="MRKKEIIEKAIEKVAMTNNTQIIKEHAWSFNPFVERYRYFDKFN
                     REYKGKITKNGIIVKVVPVRIEKKLNLTETLEFIVKGDIIYRITDKKIILEYVREFFY
                     ERGYCISFLNEKKGKMLVKGRNIIGFFENQQFGEIEIAKKINFWEVKIRDENKEKPYF
                     CSPNFQFILKLKNEIKEGESLLIKYDEEEITSTSLSPFAIIKRAIYGFKSPNLRAFYS
                     EDGLFPEVQHLIRLSVLANFSAAIVNASIFVRIAERLKGNTWYTLASGLLAFVRAPAE
                     IYYMKKSLKLSENLPSTKVQQVLDSEKFKRIIESKEIRTWRNFERELFEILRKKGISN
                     FSNEYGKIKKDIMGYVERNEKEKILSVFEKFHLTDDEKEKISSFIKFLVPISLNDFKK
                     KVMEEIKYLCENKVLKEEAEKAIEDYIDMIFVYNLERFTFNKSYKLLLDKIKQEERTP
                     TNEEITSLINGCKRGILLYRVSDLFLFFSLYAIGYLASFYIGLPDFAIPIYYIFNGMT
                     ANIIVAALNRYESQISLQVLYRELDSAPTITSAADAWNEYNSHLMRIWAIFSSLGLGL
                     GIAGKLLADKTGNFSRYFVEGIAFFMYIQAFRERLFYYEKLEKRKKRGEE"
     gene            8202..9533
                     /locus_tag="PLW95_06795"
     CDS             8202..9533
                     /locus_tag="PLW95_06795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOV22366.1"
                     /translation="MNNYRTVCLMGLDEIPLDNLKELENEFSCQIQRTNTIPEKGILL
                     ILDLEALKENVISEIKNKNIPYIVVCECDEKEVRRVINSGAIDFLTKDKEKNYIKIFP
                     DYLKHILSQLVKQFYAEKQVFSKWSEYALFGIYVFGEDLKFRYVNPGFLKMLGYTADE
                     IYKKHDALSIIYPEDREMTREKIRERFEGREKIVEYPNRFVHKNGDVKWVYIKGFVTK
                     LDGEKVIIGTVSDITEIKKYQEELEKEKAELEETLRESIYAINKIFEVRDPYTSSHQI
                     RVAEIAEKVAKELNFNEKSLKEILWAGLLHDIGKIAIPSDILSKPSKLNDIEYSLIRL
                     HPITGYNILKEIHQVGKIAEIVYQHHERLDGSGYPRGLKKDNIIFEAKLLATIDVIEA
                     MGSHRPYRAARKVEEILEELNSGRGKKYEPIIVDKVIELMKKGEIIITPKE"
     gene            complement(9517..9861)
                     /locus_tag="PLW95_06800"
     CDS             complement(9517..9861)
                     /locus_tag="PLW95_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOV22367.1"
                     /translation="MNKIEIGKSGEEEAINFLKRNKYKILNRNFKTKFGEIDIIAKKN
                     GEIVFIEVKTRNTTLFGYPEEAIDKRKINHIQKVATYYLQRNKIKIPFKFEVLSILTK
                     NGRKEINVIPLE"
     gene            complement(9858..10190)
                     /gene="rplS"
                     /locus_tag="PLW95_06805"
     CDS             complement(9858..10190)
                     /gene="rplS"
                     /locus_tag="PLW95_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOV22368.1"
                     /translation="MNKQIEEIEKEIIKNKEIPEFWPGDDISIHYKIKEGEKERIQIF
                     TGTCIGRKGSGAQETFTVRKISYGEGVEKVFPLYSPNVVKIEVIKTRKRNRLAPRAKM
                     YYLRNKNK"
     gene            complement(10183..10869)
                     /gene="trmD"
                     /locus_tag="PLW95_06810"
     CDS             complement(10183..10869)
                     /gene="trmD"
                     /locus_tag="PLW95_06810"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009019 - tRNA
                     (guanine-N1-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOV22369.1"
                     /translation="MRIYILTIFPDVFTYFDVSIIKRAKEKGIIEINVLDLRKFTKDK
                     HKKVDDKVYGGGKGMVLMCQPIFDGVEFLKKENKDAKVILTTPQGKLYNQEMAKELSK
                     EKSLIIICGHYEGVDERVTKICDYEISIGDYILTGGEIPAMVIVDSVIRLLPGVLPEE
                     VPVSDSFYNPIFDWPAYTRPENFRGMKVPEILLSGNHKKIEKYRKEKAIEKTKEKRPD
                     IYKKYGGRNE"
     gene            complement(10866..11129)
                     /gene="rpsP"
                     /locus_tag="PLW95_06815"
     CDS             complement(10866..11129)
                     /gene="rpsP"
                     /locus_tag="PLW95_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOV22370.1"
                     /translation="MAIRIRLVRTGKKNKAFFRIVAVDRKKDGRGDVKEILGHYDPLG
                     DPEIVEIKEERISYWFEKGAIPTQTVKSLCKKKGIFIPPNVDK"
BASE COUNT         4196 a         1346 c         1918 g         3693 t
ORIGIN      
        1 ttattataaa actgactggt cagtcataat gaataaaaaa aaattaatat taacagtagc
       61 agaaaaaata ataagtgaaa aggggtattc aaatacaaca attgaggaaa ttacaaaaag
      121 agcaaaaatc ggaaaaggaa cattttataa ttattttaaa aacaaagaag aactttttta
      181 tttaataatt aaagaaggtc ttgataattt agtaattgag attaaggact caattgaaga
      241 cattgatgat ttttttgaaa ggataaaaat gggaatagaa atgtatttaa aatatcatca
      301 gaaacacttt tatttcttta aaattttgat tcaagaaaag ccatttttga aaaataaaaa
      361 atttataaat ttctgggagg acttttttga tagatgggat tttataaaaa aggacttaaa
      421 aaaacagata gaaagaaata aaatagtaga tattgaacca gatgatattc tttattctct
      481 gcttggaatc ctgcatggga atattcataa atggcatata agtggaagaa aatattgtct
      541 tgttgaaaaa aaagatgcta tatttaatgt gtttataaat ggaataagga ggaaataaaa
      601 tgagatactt tttaattatg cttttgtgtt ttccatgttt tgttttttct gagaactttt
      661 ttactcttcc agatgcaata aaagttgcaa ttaacaataa tcttgaaata aaaatttcag
      721 aagataaaat agagcaggca tattataaaa aagaagaagt aaaaacatat tttttcccaa
      781 aatttacttc atcgtttgcc tatacatatt caggagatag tgaaaaattt aggacagatg
      841 ataatttata taatctttca ttatcattat ctcaaccaat ttatactgga aaaagaatta
      901 aagagacata tgagatatca aaagaaaacc ttgataatgc aaaattggag tataatgttc
      961 aacttcagaa tttaattttt aatgttaaaa aagggtattt ttctatatta caggcaaata
     1021 aatttttaaa aacaagtgag agatataaac aaatgttaga aaaacatatg gatgatgcaa
     1081 aaaaattatt tgaacaggga cttgtaacaa atcttgatat tttaagaaca gaggtctctg
     1141 taaaaaatgc agaaacacaa ataatccagg cagaaaattt aatcaaaatt tcaaaatcaa
     1201 attttaatta tatcttgaat aatccaatag ataatgaatt tgaagttgaa gatgtacttg
     1261 aaataaaagg agataaaagg gattatgaat actggaaaac actggcatta gaaaacagac
     1321 ctgaaattaa gagtatggaa aaacttttat caatttataa tagagcaata aatgtagaaa
     1381 ggagtaattt atatccgcag gtctatcttt tttacaatta taatactgat agaggaatac
     1441 aggtatctcc aaatagttgg aatccaaact ggaatactgg aattgctctt actcttgata
     1501 tatggaactg gggagaaaca aaagataaag taaagcaagc agaaagtgaa aaaaaacaag
     1561 tagaaaaaca acttgaaatt acaaaaaaat caattgaatt agaagttaaa aactgttatt
     1621 tgaacttact tgctaatgaa aaaagtattg aagagcgact taaacaaatg gaaacagcag
     1681 aagaaaactt aagagttgca aacctcctgt accaggaagg gatggcaaca acaacagagg
     1741 taattgaagc agcaacttct ttgacagaag cgacaaataa ttattctcag gcattatatg
     1801 attatcacat ttcttttgct gaacttgaaa aggcaagtgg gaaaggagtg gaataatgaa
     1861 aaagaaaatt ttacttattt gtattttaat tctttttgtt attggattgg gtaatgcaat
     1921 tgtaaaaaat ataaagaaaa gaagaggtaa tgaagtaaag gaagaagtag ttaaaattcc
     1981 tgttaaagta atgcaggtaa aaagaggaga tgtcagggaa gttgtttctg cttccgggga
     2041 tattcagcca tggtctcagg ttacagtttt ttcaaaagtt acaggacaag tagaaaaatt
     2101 atttgttgat aaagggtatt atgttaagaa aggtggacta attgcccagg ttgactatag
     2161 aaataatgct ttagcagtaa aacaaataga aaatcaatta tcagcagcaa gaataaatta
     2221 tgagaattta aagaaggatt atgaaagaat gaaggactta tttaaccaaa aagttatatc
     2281 tgaaaagaaa ttggatgata caagaactgc atgtgaatcc gcctatcacg aagttgaaag
     2341 tttagaagca caacttgaac ttgcaaaaat aaaattagga gatacaagaa ttacctctcc
     2401 tataaatggt gtagtagcag aaaaatttat tgatgaagga gaaattataa ctgaagcatc
     2461 catgtcaaaa agtgctccta ttgtaactat cgttgatatt gatacagtaa aaattgttgt
     2521 taatgttgga gaaaaaaaaa taagtaaagt aaaaaaaggt cagtcagttg aagtaaaagt
     2581 agatgcttat cctgacaaat tgtttatagg taaagtttat aatatttcac caattacaga
     2641 cccacaaaca agaacaacag aagttgaaat ttctgttaaa aatcaaaatt atcttttaaa
     2701 accagggatg tttgcaagaa caaatattat tattacttct cataaaaatg ttatagttat
     2761 tccttttgat gcaatcgttg aaagagaagg taaaaaatgt gtttttgttg tagagggtaa
     2821 ttatgcaaaa atgaaagaaa tagatactgg attacaggaa gtagacaaaa tagaagttac
     2881 atccggattg acagaaaatg aaaatttaat tattgaaggg caaaatacag taaaagatgg
     2941 aattgaagtt atgttggtag agtaagcaaa agcgtaaagt gtaaagtgta aattagggga
     3001 gaataaaaat gagtatacca gaaataggag ttaaaaggcc agttactact gctatgattt
     3061 ttataggtat tcttatactt ggcggtgttt ctttgacgcg tttaggtctg gatttattac
     3121 ctgatattga aattccttca attatggttc ttacgactta cgaaggagca gggcctgaag
     3181 aagtagaaga aagaattact cgtgttgcag aagaacaact tgcaactgtt gataatcttg
     3241 ataaaattga ggcattttca caagagggta tgtcaggtat tacattaaga tttaattggg
     3301 gaacaaattt agacaatgca gtcaatgata taagggataa acttgacctt gttaagccaa
     3361 tgcttcctga tgaagcagat gacccaataa tttataaatt cgatgtttct atgatgccta
     3421 tattggtata tggagtaagt gctgaccaaa catacccacg acttaaaaaa cttcttgaag
     3481 ataatgtttg tattccatta gaaagtgtta caggggttgc gagtgcagtt gttcgaggtg
     3541 gacttgaaag ggctatattg gttgaattag atgctgaaag attgaaggca tataacattt
     3601 ctatagcaca ggtggaaaat gccatagtag cagcaaactt atctacacca ggtggacata
     3661 taaaagaggg taaatttgat tatattgtca gggtccctga agaaattaaa ataaaagaaa
     3721 tagaagatgt tgcaataggt gtttataatg gaattccaat aaaaattaaa gatatagcag
     3781 aagttaaaga tagttttaaa gaaataacac aggaaacaca tgtaaatagg cggtctggaa
     3841 tgactataat gattcaaaaa gagtcagatg caaatacagt aaatacatgc agggctgtat
     3901 ttaaaaaact ggaagcatta aaagaaacat taccttctga tgtaaaattt tctcttgtaa
     3961 gtgattcatc agaccagatt gtaaaatcaa taaataattt aaaaaattct cttataagtg
     4021 gtggaatact tgttatactt gttatttttt tatttttggg caatatccgt tcttcactta
     4081 ttattgcatg tgctatccca atttctctta ttattacatt tgtttttttg tatatggctg
     4141 gttatacaat aaatattatt tctctatctg ctttagcaat tgctatagga atggtagtag
     4201 atgcatcaat tgttgttcat gaaaatattc accgtcattt agaaaatggt gaagaagtca
     4261 taaattcatg tattgttggt ggtaatgaag tagcaggttc tatatctgct tctgttttat
     4321 gtatggtagc aatatttttg cctataatgt tcacaggtgg tcttactgct attttctttg
     4381 gtcaacttgc ctttgtttgt tcagcagcac ttattgcttc tctatttaat gcgcttatgc
     4441 ttattccaat gctttcatct aaattacttt ctataccaca aaaggaagat acaaatggaa
     4501 agaaaaaagg aattttggat aaaataactt cttttgctga ggtatggttt aaaaaattgg
     4561 ataatgctta tggagaccta ttaagatggt gtttaaggaa tagaaaaaaa gttgtatttg
     4621 gtagtgcaac tttacttttt ttaagtttac ttcttgtcaa atttattgga actgaattta
     4681 ttcctgaaat ggatgaagga agggtagcgt ttaaaattac actcccgata ggaacaagat
     4741 atgaaaagac aggagaaata acaaaagggg tagaagatat tatatataaa aatgtccctg
     4801 aaattcaaac aggtttttct cgatggggtg aaggtggcgg aggactttct tctctattgg
     4861 gtggagaaga aggttctaat atagggaatg taactcttaa acttattgac aaagaaaaaa
     4921 gaaatagaac accaagacaa attgcttatg atataaagga taaacttaat gtttttcctg
     4981 atgcagaagt aagatataat ccttcatcta tgtctgcaat gatgtttgga ggagcaaaac
     5041 cgctatcagt agaagtgagg ggatatgatt ttgaccaggc aagaacttta tctcataaag
     5101 tagaagagat attgaaaaat acaaaaggtg taagtgatgt agaaatatca agaaaagaag
     5161 gtagaccaga attacaggtt ttgatagata gagaaaaggc atctacactt gggctgaatg
     5221 ttgcaactat ttctcatatg gtagagacat cttttaaagg aaaggtatca acttattata
     5281 gagaaagtgg agatgagtat gatattgagg taaaactaag agaggaagat agaaccaaaa
     5341 aagagaatct ggaaaatttg tttattacac ttccttctgg caaaaaaata catttaactg
     5401 aaatagcaga aataagagaa acggcaggtc ctttaaagat agaaagaaag ggaagagaac
     5461 gaatggtaag agtagaagga gaaatttatg gaagggattt aggaagtgta gtaaatgaaa
     5521 ttaatgaaaa attagaaaaa ttacaacttc cacagggttt ttctttttat tttggtggtg
     5581 aatatgaaga acaacaaaaa agttttagac aactaacatt tgctttgata ttagggataa
     5641 ttcttgtatt tatgattatg gcagcacaat ttgagtccat tattgacccg tttgttataa
     5701 tgtttgctat tccatttgct cttattggag ttgtttgggc attattttta tcaggtaata
     5761 ccatttctat tttctctttt attggtgtaa ttatgatagt aggaattggc ttggaaacag
     5821 gtattgttct tataagtttt ataaaggatt taagaaaagc aggtgttgaa ttacatgagg
     5881 cagttgtaca ggcagggaaa ttgcgactga gagcagttct tatgacaact cttactacaa
     5941 tttttggtct tcttccttta gcattatcaa aaggtgaagg tgcagaaatg tgggttccat
     6001 tgggttggtc tcttattggc gggcttcttg tatccttcct tttaactcta ttctttgtcc
     6061 caattcttta tactatttat gaagaaaaga tattgcaaag aggtaagaaa aaagcataat
     6121 tcttcatagt attggaaata aataactgga atgtccacaa aaatcttctc tatttattca
     6181 aacaaattcg tttcaaaatt aactttttgt taataattgt tgacagatgt gtattaaaaa
     6241 ggaaaataaa agtatgagaa aaaaagaaat aattgaaaaa gcaattgaaa aagttgctat
     6301 gacaaataat actcaaatta taaaagagca tgcatggtct tttaatccct ttgttgaaag
     6361 atatagatat tttgataaat ttaatagaga atataaaggc aaaattacca aaaatgggat
     6421 tattgtaaaa gttgttcctg taagaataga aaagaaactt aaccttactg aaacattaga
     6481 atttattgta aagggagata ttatatatag aattactgat aaaaaaatta tccttgaata
     6541 tgtcagagaa tttttttatg agaggggata ttgtatatcc tttcttaatg agaaaaaggg
     6601 gaaaatgctg gttaaaggaa gaaatataat aggatttttt gaaaatcaac aatttggcga
     6661 aattgaaata gcaaaaaaaa taaatttttg ggaagtaaaa ataagagatg aaaataaaga
     6721 aaaaccatat ttttgttcac ctaattttca atttattcta aaattaaaaa atgaaataaa
     6781 agaaggtgag tcccttctaa taaaatatga tgaagaagaa ataacatcaa cttcactttc
     6841 accttttgca attataaaaa gggctatata tggatttaaa agtccaaatt taagggcatt
     6901 ttactcagaa gatggccttt ttccggaagt acaacattta ataagattaa gtgtccttgc
     6961 aaatttttca gcggcaattg taaatgcaag tatttttgtt agaattgcag aacgtcttaa
     7021 aggtaatacc tggtatactc ttgcttctgg acttcttgct tttgtcagag cgcctgctga
     7081 aatatattat atgaaaaagt cccttaaatt gagtgaaaat ctcccttcaa caaaagttca
     7141 gcaagtactt gattcagaaa aatttaaaag aattattgaa agtaaagaaa taagaacatg
     7201 gagaaatttt gaaagagaac tttttgagat tttaagaaaa aaaggtattt caaatttttc
     7261 aaatgaatat gggaaaataa aaaaagatat aatggggtat gttgaaagaa atgaaaagga
     7321 gaaaatactt tctgtttttg aaaaattcca cttaactgat gatgagaaag aaaaaatttc
     7381 atcttttatt aaattccttg ttccaatatc tttaaatgat tttaaaaaga aagtaatgga
     7441 agaaataaaa tatttatgcg aaaacaaagt tctaaaagaa gaagcagaaa aagcgataga
     7501 agattatatt gatatgattt ttgtttataa tcttgagaga tttacattta ataaaagtta
     7561 taaattactt ttggataaaa taaaacaaga agaaagaact ccaacaaatg aagaaataac
     7621 atctctaata aatggatgta aaagagggat tttactatac agggtctctg accttttttt
     7681 atttttttct ctttatgcaa ttgggtattt agcaagtttt tatattggac tgcctgattt
     7741 tgcaattccc atttattata tttttaatgg gatgactgca aatattattg tcgctgcgct
     7801 taacagatat gaatcacaga ttagtctaca ggttttatat agagaacttg attctgctcc
     7861 aacaataacc agtgctgcgg atgcgtggaa tgaatataat tcacatctta tgaggatatg
     7921 ggctattttt tcttctcttg gacttggact tggtattgct ggaaaacttc tggcggataa
     7981 aacagggaat ttttcgagat atttcgttga aggaattgcc ttttttatgt atatacaggc
     8041 atttagagaa agattatttt attatgaaaa acttgaaaaa agaaagaaaa gaggagaaga
     8101 ataaaaactt aaaaaagaaa taagatgtac cttaaaaccc cataggttaa aaagtaataa
     8161 ctatattggc aataaaatat attttaaggt ataattaaga aatgaataat tacagaacag
     8221 tttgtttaat ggggcttgat gaaattccac tggataattt aaaggaatta gaaaatgaat
     8281 tttcctgtca gatacaaaga accaatacca taccagaaaa aggtattttg ctcatattgg
     8341 atttggaagc actaaaagaa aatgtcattt ctgaaattaa aaataaaaat attccttata
     8401 ttgttgtgtg tgaatgcgat gaaaaagaag taaggagagt tataaatagc ggtgcaattg
     8461 attttcttac aaaagataaa gagaaaaatt atataaaaat ttttcccgat tatttaaagc
     8521 atattctttc tcaattagta aagcagtttt atgcagaaaa acaggtcttt tctaaatgga
     8581 gtgaatatgc actatttggt atttatgttt ttggtgagga cctaaaattt agatatgtca
     8641 atccgggatt tttgaaaatg ttgggatata ctgctgatga aatttataaa aaacacgatg
     8701 ctttaagtat aatttatcca gaagatagag aaatgacaag agagaaaatt agagaacgat
     8761 ttgaaggaag agaaaaaata gttgaatatc caaatagatt tgttcataag aatggtgatg
     8821 tgaaatgggt ttatataaag ggttttgtaa caaagcttga tggtgaaaaa gttataatag
     8881 gcacagtttc agatataaca gaaataaaga aatatcaaga agaacttgag aaagaaaagg
     8941 cagaacttga agaaacactt agagaaagta tttatgcaat taacaaaatt tttgaggtaa
     9001 gagacccata tacttcttct catcaaataa gagttgcaga aattgcagaa aaagttgcta
     9061 aggaattaaa ttttaatgaa aaaagtttaa aagaaatctt atgggcaggc cttttacatg
     9121 atatcgggaa aattgcaatt ccttctgata ttctttcaaa accatcaaaa ttaaatgata
     9181 tagaatattc tttaatacga cttcatccta taactggcta taatatttta aaagaaatac
     9241 atcaagtagg gaaaatagca gaaattgtat atcaacatca tgaacggctt gatggaagcg
     9301 gatatccgag gggattgaaa aaagataata taatttttga ggcgaaatta ctcgcaacta
     9361 ttgatgtaat tgaagcaatg ggttcccatc gtccttatag agcagcaaga aaggtagaag
     9421 agatattaga agaattaaat tcagggagag ggaaaaaata cgaaccaata atcgtagata
     9481 aagtgattga attgatgaaa aaaggagaaa taattattac tccaaaggaa taacatttat
     9541 ttctttcctg ccgttttttg tcagtataga taatacctca aatttgaaag gaatttttat
     9601 tttatttctt tgtaagtaat aagtagcaac tttttgtata tggtttattt ttctcttatc
     9661 aattgcttct tctggatatc caaagagagt tgtattcctg gtttttactt ctataaaaac
     9721 aatctctcca tttttctttg caattatgtc aatttcacca aactttgttt tgaaatttct
     9781 atttaggatt ttatatttgt ttctttttaa aaaatttatt gcttcttctt ctccgctttt
     9841 tcctatttct attttattca tttattttta tttcttaaat aatacatttt tgctcttggg
     9901 gctaaacgat ttctttttct tgttttaata acttctattt taacaacatt aggagaatac
     9961 agaggaaaaa ctttttccac tccctctcca taagaaattt ttctaacagt aaatgtttct
    10021 tgtgcaccac taccttttct tccgatacat gttcctgtaa atatctgtat tctctctttt
    10081 tctccctctt ttattttata atgtatagaa atatcatcac caggccagaa ttcaggaatt
    10141 tctttatttt ttattatttc cttttcaatt tcttcaattt gtttattcat ttcttcctcc
    10201 atatttttta taaatatcag gtcttttttc ttttgttttc tcaattgctt tttcttttct
    10261 atatttttct atttttttgt gattccctga taataaaatt tctgggactt tcatacctct
    10321 aaaattttca ggtcttgtat aggcaggcca gtcaaaaata ggattataga aactatcaga
    10381 cactggaact tcttctggta agacacccgg tagtaatctt ataacactat caactataac
    10441 cattgcagga atttcccctc ctgttaaaat ataatcacca attgaaattt cataatcaca
    10501 tatttttgtt accctttcat caactccctc ataatgaccg cagattatta taagtgattt
    10561 ctctttgctt aattcttttg ccatttcctg gttatataat tttccctgtg gtgttgttaa
    10621 aataactttt gcatctttat tttctttttt taaaaattca acaccatcaa aaattggttg
    10681 acacattaaa accatccctt ttcctccacc ataaacttta tcatcaactt ttttatgttt
    10741 atcttttgta aattttctta aatccaatac atttatttca ataattcctt tctcttttgc
    10801 cctttttata atacttacat caaaataagt aaaaacatca ggaaaaattg taaggatata
    10861 aattctcatt tatcaacatt tggaggtata aatatacctt ttttcttgca gagtgattta
    10921 actgtttgag ttggtattgc ccctttttca aaccagtagg atattctttc ttctttaatt
    10981 tcaacaattt ccgggtcacc aagagggtca taatgaccaa gtatttcttt aacatctcct
    11041 ctgccatctt tttttctatc aactgcaaca atcctgaaaa atgccttatt ctttttccct
    11101 gttcttacaa ggcgtattct tatagccata attaactcct tttatatttt tct
//
LOCUS       DAOOVW010000051        10707 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_3974_length_10707_cov_8.466485, whole genome shotgun sequence.
ACCESSION   DAOOVW010000051 DAOOVW010000000
VERSION     DAOOVW010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10707
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3974_length_10707_cov_8.466485"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            249..710
                     /locus_tag="PLW95_06820"
     CDS             249..710
                     /locus_tag="PLW95_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HOV22371.1"
                     /translation="MELIKDVQIKNLKVIPDERGILIEILRNDDEIFEKFGQVYITTA
                     YPGVVKAWHCHQFQNDNLTVIKGMAKIVLCDLRKESPTYKKINEFFVGIYNHILIHIP
                     AGIYHGFKCISEEECIVLNIPTNTYNYKNPDELRLPFDTDKIPYNWERRNY"
     gene            763..960
                     /locus_tag="PLW95_06825"
     CDS             763..960
                     /locus_tag="PLW95_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22372.1"
                     /translation="MGIKCRDCNKKAEVNIQKIWIKWDYNYKDDSYSDTFEALLDAEE
                     PIGENNLHFCKKCFYKWMYWE"
     gene            942..1877
                     /locus_tag="PLW95_06830"
     CDS             942..1877
                     /locus_tag="PLW95_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22373.1"
                     /translation="MDVLGIMINDIYSNSCWIMSSNKENFKYAIEIYNRTWGVQDKWF
                     PQWKALERENIIFFYISDNVKKIIGCGKIENKFIQREPLWPDEIRENKVKYPLRFTFK
                     VDILLSENEWNNRGIDIIKFMKENWGRGKFAELLRGGVNFIQHKELINFLIREFNKKL
                     SYEILFDKIPIQYEEEVIEEQDLHKQLIELIVQIGNMNNLLSQKEYKIDNMILDVVWR
                     RIEKGSPTYVFEVQVGGDVYHALSKLKHAFDLWNSNVFLIIHSKRDVIKADQLLGGTF
                     HEIKERVKIINSKDIRELYKRKKSWVEFEKKLGIL"
     gene            1888..2070
                     /locus_tag="PLW95_06835"
     CDS             1888..2070
                     /locus_tag="PLW95_06835"
                     /inference="COORDINATES: protein motif:HMM:NF018054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22374.1"
                     /translation="MNFIRETKTPENVKKVAIVDGIIWFAKKGKIKEQIENIKDDELV
                     FSALLLDEFLKGGIIK"
     gene            2067..2798
                     /locus_tag="PLW95_06840"
     CDS             2067..2798
                     /locus_tag="PLW95_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOV22375.1"
                     /translation="MKGVILAGGLGKRLYPLTKITNKHLLPVYNKPMIFYPIETLIDA
                     GITDILIVTGGNFAGDFLQLLGNGKIFGLPNLNYTYQEGEGGIADALSLAENFSKDDK
                     IVVILGDNIIEKSIKEQVEIFKKQKKGARILLKKVENPERFGVVEFNKKGKIKRIIEK
                     PEKAPTDYVVTGIYMYDRYVFEIIKTLKPSKRGELEITDVNNRYLKNGELTYGIIDGW
                     WTDAGTFDSLLKANNLVAKKTKKRK"
     gene            2795..3808
                     /gene="rfbB"
                     /locus_tag="PLW95_06845"
     CDS             2795..3808
                     /gene="rfbB"
                     /locus_tag="PLW95_06845"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010494539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOV22376.1"
                     /translation="MKIVITGGCGFIGSNFIRYIIKKYPEYEILNIDKLTYAGNPENL
                     GDIENNKNYKFLKKDICDKSIIDDISAGSFGKNYDVIINFAAESHVDRSIGNPEKFLK
                     TDIFGTFNLLEIVRKLNFKRFIQISTDEVYGSIQKGSFDESAPLNPSNPYSASKGSAD
                     LLVLSYFKTYKSPVVIIRSTNNYGPYQYPEKFIPLFIINAIRNKKLPLYGDGRNVRDY
                     LFVLDNCKGIDIVLHKGKEGEIYNISAGNEKENIYVANKILEYLGKDESLIEFVKDRP
                     GHDKRYSINSDKIRKLGWKDEVSFEDGLKRTVEWYKENVEWWKKILNRQSYKNYYKKQ
                     YRK"
     gene            3805..4644
                     /gene="rfbD"
                     /locus_tag="PLW95_06850"
     CDS             3805..4644
                     /gene="rfbD"
                     /locus_tag="PLW95_06850"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOV22377.1"
                     /translation="MKILITGGKGLVGSYLTKILSEKYNVISTGREEVDIVNYDKSLD
                     FIKNIKPEVIIHCAAFSNVDGCEVEQEKAYKVNSIGTLNISKICQEVKSKLIYISTDF
                     VFNGEKKKPYSEIDIPEPINHYGKTKLIGEYYVSHLLNDFVIVRSSRIFGKSGKNFGS
                     KLPTLLKNDQKVKLTTDIINSPTYAFELAKAIEFLIKQNYKGIINICNEGECSWYEFG
                     KEVIKILKKKERRIEKIKFSQFEYKAKRPKYSTLDVSLLKTLGYYPVSWQLSLRKFLS
                     DQF"
     gene            4704..4775
                     /locus_tag="PLW95_06855"
     tRNA            4704..4775
                     /locus_tag="PLW95_06855"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4737..4739,aa:Glu,seq:ttc)
     gene            complement(4859..5158)
                     /locus_tag="PLW95_06860"
     CDS             complement(4859..5158)
                     /locus_tag="PLW95_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22378.1"
                     /translation="MEKEVKRYRMRPNEYFCLSPEETLTIGISPGEQIIEEIKLIRNP
                     YFGYIMYASIRELPESEIKKIPFKERYEFLFESRKEAMEFIKTIGVDILHLYRVI"
     gene            5247..6725
                     /locus_tag="PLW95_06865"
     CDS             5247..6725
                     /locus_tag="PLW95_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOV22379.1"
                     /translation="MKNLNALKEFKGIANLAGPLMLIEGVEGAGYGELVEVLTPDGMR
                     LGRILEIEQDKALIQVFEGTRGISPDRAKVRFTGKGIELGVSPDILGRVFTGMGKVRD
                     GGPEIIPEKYLNINGEPINPTRRDYPSEFIQTGISAIDGLNSLVRGQKLPIFSGSGLP
                     HNLLATQIVKQAKIISKEETKFAIVFVAMGITFEEAEFFLKEFRDAGAIERTVTFINL
                     ADEPAIERTATPKVALTAAEYLAFENDMHVVVILTDMTNYAEALREISAARNEIPGRR
                     GYPSYLYTDLSTIYERAGRIKGKKGSITQIPILTMPEDDKTHPIPDLTGYITEGQIIL
                     SRQLHLKGIYPPIDVLTSLSRLRDKGIGVNKETGEIHTRQDHPAIASQLFASYARGKE
                     AEELAMVLGEASLTEEDIAHLNFAKEFEEKFLSQGIDENRTIQQTLDIGWQLLRTLPM
                     DTIKRIPPEIMEKYWKNENKSKSNENGVIETEKKSTGSKERT"
     gene            6646..7269
                     /locus_tag="PLW95_06870"
     CDS             6646..7269
                     /locus_tag="PLW95_06870"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOV22380.1"
                     /translation="MKIKVNPTRMELLKLKRRVQVAKRGHKLLKDKEEQLLIEFRKLL
                     DEIKDKRKKVEEELYQYYLKVVELKGITQKKKWENLISSPFIKTEVKVIKKRIFNIPV
                     KEINFEVKETLTDYFSSPYYQFLIEKGTEVINRLFYLYHLENKLISFTEEIERTRRRV
                     NALEYVLIPGIEEAIKFIKFKLEENERANLTKLKHLQLIELRHTKST"
     gene            complement(7313..8440)
                     /locus_tag="PLW95_06875"
     CDS             complement(7313..8440)
                     /locus_tag="PLW95_06875"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR00045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOV22381.1"
                     /translation="MKLLICPDSFKGCFTSLEGSKIIKNVFKKYFPESTPILFPLADG
                     GEGSIEVLNKFIKGKFVFIESSDPLGRKIKTKYLKKGKAVYIELAKEAGLTFLKEEER
                     NPLETTTYGVGEVILRAIEDGCENISIFVGGSATNDAGIGALSAIGVKFFDEKGKTIF
                     PGKGKDLVKIRGTDISSVDKRIFNIKFTILTDVKNPLYGKNGASYIYAWQKGANSKEV
                     KILDDGLRNFSFVLKKFTGKDVSRIKGAGAAGGFASGFISIFNSKIESGIELILKKGN
                     FKEKIKNCDFVITGEGKFDKQSFFGKVVGVVIEYCKKYKVPVIILAGTVERNIYKKIN
                     EENIIVMSILPGFLSHDEAMKEGKKLLKIKAQQIFKLLKFK"
     gene            complement(8437..9483)
                     /locus_tag="PLW95_06880"
     CDS             complement(8437..9483)
                     /locus_tag="PLW95_06880"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOV22382.1"
                     /translation="MKSSVYFLPIEKISVLYDFIKWTGVFDSIGEKDTIALKIHFGNS
                     RHNNHIPAEYSKEILRVLEERKCFAFFTDTNVLYRGERENTISHIKIVKLHNYHNLGV
                     PVVIAGGSSGKDEVVLEINGKHFKNIYIAKEYSEVSGMIALTHFKGHILAAIGGTIKN
                     VGMGCASRKGKYAMHSNITPQINISKCIGCGKCVKECPVEAIELKNKKASINKKKCIG
                     CGSCIHTCPKEAINIPWSSVTPAQFQEKIVEYASGVISQYAENKFFAINFLTKIAIEC
                     DCASNPGKFLSPDIGVLVSKDPVAIDRASVDIIKKVNGKDVFEGIRPPYNYQLEYGEE
                     IGLGSISYKLIEFK"
     gene            complement(9473..10138)
                     /locus_tag="PLW95_06885"
     CDS             complement(9473..10138)
                     /locus_tag="PLW95_06885"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOV22383.1"
                     /translation="MIKGIIFDVDGVLVDTEKIHVEAWENVFCPKGITLSKDDYEKGI
                     GIADKEFLIQLKNQKKIPENLDIGRLCKEKEKYFLEIISNGVNVIDGVIKILNFLKEK
                     KFKIAAASNSKMVFVKNVLKYGGIKDFFDVIVAREGIKEAKPEPEIYIKTLELLKLKK
                     NEVIVFEDSEVGIESAKRAGIFCVGIETTQQYDKLKKSDIILKKMDIEKIKNIIKGME
                     SEK"
     gene            10318..10647
                     /locus_tag="PLW95_06890"
     CDS             10318..10647
                     /locus_tag="PLW95_06890"
                     /inference="COORDINATES: protein motif:HMM:NF012684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HOV22384.1"
                     /translation="MKIKKDEIKTYFYKSRGPGGQRKNKKETAVKIVHIPTGIIVRGT
                     ESRSQIENRKLALKRLEEKLEKLNKKPKKRIPTKKSEKVKQKQIEEKRKKSEKKKLRK
                     HINPYEI"
BASE COUNT         4011 a         1465 c         1645 g         3586 t
ORIGIN      
        1 ggcagagcac cgccctttta aggcgggggt ggcaggttcg agccctgcgc ggcctattaa
       61 aacaggtaaa acatagacaa aaaaaataat tggggtaaaa tataaatata ataaacgaat
      121 tagataaaag agagaacacc tcctcagtac cccctttaag ggagaggttc caaaattata
      181 ctgggacctc tcccattttt tttatattat ttttttaaaa ttctaaaact tctgataaaa
      241 tataaattat ggaattaata aaagatgtcc agattaagaa tttaaaagtc attcctgatg
      301 aaagaggaat attaatagag attttaagaa atgacgacga aatttttgaa aagtttgggc
      361 aggtatatat aacaactgct tatccaggcg ttgtgaaagc atggcattgt caccagtttc
      421 aaaatgataa tttaactgtc ataaaaggaa tggcaaaaat tgtcctttgt gatttgagaa
      481 aagaaagtcc aacttataaa aaaattaatg agttttttgt tggtatttat aatcatattt
      541 taattcatat tcctgctggt atctaccatg ggtttaagtg catatctgaa gaagaatgta
      601 tcgttttaaa tatccctaca aacacatata attataaaaa tccagatgag ttacgactcc
      661 catttgatac agataaaatt ccatataact gggaaaggag gaattattaa gtgaaagggg
      721 taattcaaaa aaaattaata ttaaaaagag gtgatgtaaa aaatgggaat taaatgtaga
      781 gattgtaata aaaaagctga agtgaatata caaaaaattt ggataaaatg ggattataat
      841 tataaagatg actcatattc ggatacattt gaagcattgt tagatgccga ggaacctata
      901 ggagaaaaca atttgcattt ttgtaaaaaa tgtttttata aatggatgta ctgggaataa
      961 tgataaacga tatatattct aattcttgct ggattatgtc cagcaataaa gaaaatttca
     1021 aatatgcaat tgagatatat aatagaactt ggggtgtgca agataagtgg tttccacagt
     1081 ggaaggcatt agagagggaa aatataatat ttttttatat ttcagataat gtaaaaaaaa
     1141 taataggatg tggaaaaatt gaaaataaat tcatacaaag ggaacctctt tggccggatg
     1201 aaatcagaga aaataaagta aaatatcctc ttagatttac cttcaaagta gatattttac
     1261 tttccgaaaa tgagtggaat aatagaggta tagatattat caagtttatg aaggaaaatt
     1321 ggggaagagg gaaatttgct gaattactgc ggggtggtgt aaattttatt caacataaag
     1381 aactgataaa ctttttaatt agagaattta ataaaaaatt gagttatgaa atactttttg
     1441 acaaaatacc aattcaatat gaagaagaag taatagaaga acaggatttg cataaacaat
     1501 taatagaatt aatagtacaa attggaaata tgaataatct tttgagtcaa aaagaatata
     1561 aaattgataa tatgatatta gatgttgttt ggagaagaat tgaaaagggt tctcctacat
     1621 atgtatttga agttcaagta gggggagatg tttatcatgc tttaagtaaa ctcaagcatg
     1681 cttttgattt gtggaatagt aatgtttttc ttataatcca ctctaaaaga gatgttatta
     1741 aagcagacca gttattagga ggtacttttc atgagataaa ggagagagtt aaaattatta
     1801 attcaaaaga tattagagaa ctttataaga gaaaaaaatc ttgggtagag tttgaaaaaa
     1861 aattggggat tttgtaatat ttttacgatg aattttataa gagaaacaaa aacacctgaa
     1921 aatgtaaaaa aagttgctat tgttgacgga attatctggt ttgcaaagaa ggggaaaata
     1981 aaagaacaga ttgaaaatat taaggatgat gaacttgttt ttagcgcttt actattagat
     2041 gaatttttga aaggaggaat tattaagtga aaggggttat tctggctggt gggctgggta
     2101 aaaggttgta tcctttaaca aaaattacta ataaacatct tcttcctgtc tataataaac
     2161 caatgatttt ttatccgata gaaacactta ttgatgcagg aataacagat attttgatag
     2221 ttacaggagg aaattttgcc ggtgattttc tccaacttct tggaaatggg aaaatttttg
     2281 gacttcctaa tttgaactat acttatcaag aaggagaggg tggtattgca gatgcccttt
     2341 ctctggctga aaacttctca aaagatgata aaattgttgt tattcttgga gataatataa
     2401 ttgaaaaaag tataaaagaa caggttgaaa ttttcaaaaa gcaaaaaaag ggagcaagaa
     2461 ttttgttaaa gaaagttgaa aacccggaaa gatttggcgt cgtggagttc aataaaaaag
     2521 gaaaaataaa aagaataata gaaaaaccag aaaaagcgcc tactgattat gttgttactg
     2581 gaatttatat gtatgataga tatgtttttg aaattataaa gacacttaag ccatcaaaaa
     2641 ggggagaact tgaaataact gatgttaaca atagatactt aaaaaatgga gaacttacat
     2701 atggaattat tgatggttgg tggacagatg caggaacttt tgattccctt ctaaaagcaa
     2761 ataatcttgt tgcgaaaaaa acaaagaaaa gaaaatgaaa atagtaatta caggtgggtg
     2821 tggatttatt ggttcaaact ttataaggta tataattaaa aaatatcctg aatacgaaat
     2881 tttaaatatt gataaactta cttatgcagg aaatcctgag aatttaggag atattgaaaa
     2941 taataaaaat tataaatttc taaagaaaga tatatgtgat aaaagtatta ttgatgatat
     3001 atccgcagga tcctttggaa aaaattatga tgtaataata aactttgcag cagaaagcca
     3061 tgttgatagg tcaataggta atccagaaaa atttctgaaa acagatattt tcgggacttt
     3121 taatttactt gaaattgtaa gaaaattaaa ttttaaaaga tttattcaaa tctctactga
     3181 tgaagtttat ggaagtattc aaaaagggag ttttgatgag tcagcccctc taaatccttc
     3241 taacccatat tctgcaagta aagggagtgc tgacctttta gttctttcat attttaagac
     3301 atataaaagt cctgtagtta ttataaggag tacaaacaat tatggtccat atcaatatcc
     3361 agaaaaattt attccacttt ttataataaa tgcaataaga aataaaaaac ttccacttta
     3421 tggagatgga cggaatgtca gagattatct ttttgttctt gataactgta aaggaataga
     3481 tattgtttta cataaaggga aagaaggaga gatttataat atatctgctg gtaatgaaaa
     3541 agaaaatata tatgttgcta ataaaatact tgaatattta gggaaagatg aatctctaat
     3601 tgaatttgtt aaagatagac ctggacatga taaaagatat tcaataaata gtgataaaat
     3661 aagaaaattg ggctggaaag atgaagtttc ttttgaagat ggactgaaaa gaactgttga
     3721 gtggtataag gaaaatgttg agtggtggaa aaaaatactt aataggcaga gttataaaaa
     3781 ttattataaa aaacagtaca gaaaatgaaa attttaatta cagggggaaa aggacttgtt
     3841 ggtagttatc tcacaaaaat tctatcagaa aaatataatg ttataagtac tggaagagaa
     3901 gaagttgata ttgtaaatta tgataagtca ttggatttta ttaaaaatat aaaaccagaa
     3961 gttattatac attgtgctgc tttttctaat gtagatgggt gtgaagtaga acaggagaaa
     4021 gcatataaag ttaattctat cgggacatta aatatatcaa aaatttgtca agaggtaaaa
     4081 agcaaactta tatatataag tactgatttt gtttttaatg gagagaagaa aaaaccatat
     4141 tctgaaatag atattccaga accaataaat cattatggga aaacaaaact tattggagaa
     4201 tattatgttt ctcatctctt aaatgatttt gtaatagtga ggtcttcaag aatctttggc
     4261 aaaagtggaa aaaattttgg ttcaaaatta ccaactctct taaaaaatga ccagaaagtc
     4321 aaacttacaa ctgatataat aaactcccca acttatgcat ttgaactcgc aaaagcaata
     4381 gagtttttaa ttaaacaaaa ttataaagga ataataaata tttgtaatga aggagaatgt
     4441 agttggtatg aatttgggaa agaagttatt aaaattttaa aaaagaaaga aagaaggata
     4501 gaaaaaataa aattttcaca atttgaatat aaagcaaaaa gaccgaaata ttctacattg
     4561 gatgtatctt tattgaaaac attgggttat tatcctgttt cctggcagtt gtctctaaga
     4621 aaatttttat ccgaccagtt ttagttatct tgacagacac acttttatca gtataatctt
     4681 aatttaagtt atattttgaa tttgccccca tcgactagcg gttaggtcac cactctttca
     4741 aggtggtagc gccggttcga gtccggctgg gggcgttttt aaaatttatt taaaacattt
     4801 ccttaaaatc aaaattttct tcatcataag aaatctcact tctatcaata acttttgttt
     4861 aaattactct ataaagatgt aaaatatcaa cccctattgt ttttataaat tccatcgctt
     4921 cttttctgct ttcaaacaaa aattcatatc tttccttaaa aggtattttt tttatttcac
     4981 tttctgggag ttctcttata gatgcataca ttatataacc aaagtaagga tttcttatga
     5041 gttttatctc ttcaataatc tgttctcctg ggcttattcc aatagttaat gtttcttctg
     5101 gagataaaca gaaatattca tttggtctca tcctatatct ttttacttct ttttccatat
     5161 aaaaatttta aaataattct ttcaatgaag tcaaatttaa tttttatatt tacacttttt
     5221 taattttttc gtataataga aaaacaatga aaaatcttaa tgcacttaaa gaatttaaag
     5281 gtatagcaaa tcttgcgggc cctcttatgc ttattgaagg agttgaagga gcgggatatg
     5341 gcgaacttgt agaagtactt actccagatg gaatgcggct tggaagaatt cttgaaatag
     5401 aacaggataa agcacttatt caggtttttg aaggaacaag aggtattagt cctgatagag
     5461 caaaagtaag atttacagga aaagggattg aattaggggt ttctcccgat attttaggaa
     5521 gagtttttac agggatgggc aaggtaagag atggaggacc tgaaattatt ccggaaaaat
     5581 atcttaatat aaacggagaa ccaataaatc caacaagaag agattatcct tcggaattta
     5641 tccaaacagg aatatcagca atagatgggc taaatagttt agttagaggg cagaaacttc
     5701 ctattttttc tggttcaggg ctgcctcata atcttttagc aactcaaatt gtcaaacagg
     5761 caaaaattat atcaaaagaa gagacaaaat ttgcaattgt atttgttgca atgggaataa
     5821 cttttgaaga ggcagaattt ttcctcaaag aatttagaga tgcaggtgca attgaaagga
     5881 ctgttacatt tataaatctt gcagatgaac cagcaattga aaggaccgct actccaaaag
     5941 ttgctttaac agcagcagaa tatcttgctt ttgaaaatga tatgcatgta gttgtaatac
     6001 taactgatat gacaaattat gcagaggcat taagagaaat ttcagcagca agaaatgaaa
     6061 ttccaggaag aagaggatac ccgtcttatt tatatacaga cctttcaact atttatgaaa
     6121 gagcaggtag aataaaaggc aagaaaggtt ctattacaca gataccaatt ttaacgatgc
     6181 cagaagatga taaaacacat cctatccctg accttactgg ttatattaca gaagggcaga
     6241 ttattctttc aagacaactt catcttaaag gtatttaccc cccgattgat gttctaactt
     6301 cactttcaag attaagagat aaaggaattg gggtaaataa agaaacaggg gaaattcaca
     6361 caaggcaaga ccatccggca attgccagtc agttatttgc ttcttatgca agaggtaagg
     6421 aagcagaaga acttgcaatg gttcttgggg aggcatcttt aacagaagaa gatattgcac
     6481 atcttaattt tgcaaaggaa tttgaggaga aatttctttc acagggaatt gatgaaaata
     6541 ggactattca acaaacactt gatattgggt ggcaattatt gagaaccttg cctatggata
     6601 caataaagag aataccacct gaaataatgg agaaatactg gaaaaatgaa aataaaagta
     6661 aatccaacga gaatggagtt attgaaactg aaaagaagag tacaggtagc aaagagagga
     6721 cataaacttc taaaagataa agaagaacaa cttcttattg aatttagaaa acttttagat
     6781 gagataaaag ataaaagaaa aaaagttgaa gaagaattat atcagtatta tttaaaagta
     6841 gttgaattaa aaggaataac tcaaaagaag aaatgggaga atttaatttc ctctccattt
     6901 ataaaaacag aagttaaggt tattaaaaaa aggattttca atattcctgt caaagagatt
     6961 aattttgaag tcaaagaaac cctaactgac tatttttctt ctccttatta tcaattttta
     7021 attgaaaaag ggacagaagt tataaatcgc ctattttatc tttatcattt agaaaataaa
     7081 cttataagtt ttacagaaga aatagaaaga acaagaagaa gagtaaatgc attagaatat
     7141 gttctaattc ctggtataga agaagcaata aaatttataa agtttaaatt ggaagaaaat
     7201 gaaagggcga acctgacaaa attaaaacat ctccaactta ttgaattgag acatacaaaa
     7261 tccacttgaa tagagaatta gccaaaataa aaggtaggaa ttagatttgt ttttacttga
     7321 attttaagag tttaaaaatc tgttgtgcct taattttcaa taattttttt ccctctttca
     7381 ttgcttcatc atgacttaaa aaccctggca gaatactcat aacaattata ttttcttcat
     7441 ttattttctt atatatattt ctttcaactg tccctgctaa aataattact ggaactttat
     7501 attttttaca atattcaata accacaccaa caacttttcc aaaaaatgac tgtttatcaa
     7561 atttcccttc acctgttata acaaaatcac aattttttat tttttcttta aaatttcctt
     7621 tttttaatat taactcaatc cctgactcta tttttgaatt gaaaattgaa ataaatccgg
     7681 atgcaaaccc acctgctgct cctgcccctt taattctgct tacatcttta cctgtaaatt
     7741 tttttaaaac aaaagaaaaa tttcttaatc catcatcaag aatttttact tctttgctat
     7801 ttgctccttt ttgccatgca taaatataag aagcaccatt tttcccatat agagggtttt
     7861 ttacatctgt taaaattgta aatttaatat taaaaattct tttatcaaca gaagaaatgt
     7921 cagtccctct aattttaact aaatccttcc ctttgccagg aaaaattgtt tttccttttt
     7981 catcaaagaa ctttactcca atagcactta aagcaccaat acctgcatca tttgttgcac
     8041 ttccaccaac aaatatactt atattttcac acccatcttc aatcgctctt aaaattactt
     8101 ctccaactcc ataagttgtt gtttctaatg ggtttctctc ttcttctttt aaaaaagtta
     8161 gtcctgcctc ttttgcaagt tcaatataaa ctgccttgcc tttttttaga tattttgtct
     8221 taatttttct accaagtggg tcagaacttt ctataaaaac aaattttcct ttaataaact
     8281 tatttaaaac ttcaatactg ccttctccac catctgcaag tggaaaaagt attggagtac
     8341 tttccggaaa atacttttta aaaacatttt ttattatttt gcttccttct aaacttgtaa
     8401 aacatccttt aaaactatca ggacaaataa gtaatttcat ttgaattcaa ttaacttgta
     8461 tgaaatactg ccaagcccaa tttcttcacc atattcaagt tgataattat aaggaggtct
     8521 tatgccttca aaaacatctt tgccatttac ctttttaata atatcaacac ttgctctatc
     8581 aattgcaaca gggtcttttg aaacaagtac tccaatatca ggagataaaa attttcctgg
     8641 attagaagca caatcacatt caatagcaat tttagttaaa aaatttatag caaaaaattt
     8701 attctctgca tactgactta taacacctga tgcatattct actatttttt cttgaaattg
     8761 agcaggagta acacttgacc atgggatatt tattgcttct tttggacagg tatggataca
     8821 acttccacat ccaatacatt ttttcttatt tatactcgct tttttatttt tgagttcaat
     8881 tgcttcaact gggcactctt ttacacattt tccacaacct atgcactttg aaatattaat
     8941 ttgaggagtt atatttgaat gcattgcata tttccctttt cttgatgcac atcccatacc
     9001 tacatttttt attgttcctc caatcgctgc aagtatatgc cctttaaaat gagttagagc
     9061 aatcattcca cttacttcac tatattcttt cgctatataa atatttttaa aatgttttcc
     9121 atttatttct aaaacaactt cgtctttgcc actacttcca cctgcaatta caactggaac
     9181 tcctaaattg tgatagttat gtaatttaac tatttttata tgacttattg tattttctct
     9241 ttctcctcta tataaaacat ttgtatctgt gaaaaaagca aaacattttc tttcttccaa
     9301 aacccttaaa atttcttttg aatattctgc tggtatatga ttattatgtc ttgaatttcc
     9361 aaaatgtatt ttaagagcaa tagtgtcttt ttctccaatt gaatcaaaaa cacctgtcca
     9421 cttaataaaa tcgtacaaaa cacttatttt ttcaataggc aaaaaataaa cactactttt
     9481 cactttccat cccttttatt atgtttttta ttttttctat atccattttt tttaaaataa
     9541 tatcactttt tttaagttta tcatattgtt gagttgtctc aatgccaaca caaaaaatac
     9601 cggccctttt tgctgattct atgccaactt ctgagtcctc aaaaacaata acttcatttt
     9661 ttttcaattt aagcaattct aatgttttta tatatatctc cggttctggt tttgcttctt
     9721 tgataccttc tcttgctaca ataacatcaa aaaaatcttt tattcctcca tattttaaaa
     9781 cattttttac aaaaaccatt tttgagtttg atgctgctgc aattttaaat tttttttctt
     9841 tcaaaaaatt tagtatttta ataacaccat caataacatt aactccatta cttataattt
     9901 ctaaaaaata tttttctttt tctttacata atctaccaat atctaaattc tctggtattt
     9961 tcttttgatt ttttaattgt ataagaaatt ctttatctgc aattccaatt cccttttcat
    10021 agtcatcttt acttaaagtt attcctttgg gacaaaaaac attttcccat gcttcaacat
    10081 gtattttttc tgtatcaaca agtaccccat ctacatcaaa aattattcct tttatcatct
    10141 ttttataatg gtgaagtgaa cattttcatt ttatattttt acccttcaag agttcacttt
    10201 tcaatactgt cttatcaaat gaaaattcat tccatcatat agcttatatt tctctttttc
    10261 ctttgctgcc ttgactattt agaaattact tataaaattt ctaaatacaa agggaaaatg
    10321 aaaataaaaa aagacgaaat aaaaacatat ttttacaaaa gtaggggtcc tggtggtcag
    10381 agaaaaaata agaaagaaac agcagttaaa attgttcata ttccaacagg aattattgta
    10441 agaggtactg aatccaggtc tcaaattgaa aatagaaaat tagcactcaa aagattagaa
    10501 gagaaacttg aaaaattaaa taaaaaaccg aaaaaaagaa ttcctacaaa gaaaagcgaa
    10561 aaagttaaac agaaacaaat tgaagaaaaa agaaaaaaaa gtgaaaagaa aaaattaaga
    10621 aaacatatca atccatacga aatataaaac tatatattaa ggagttttat agcattttct
    10681 cttgcaactt tattgaaaac aatttct
//
LOCUS       DAOOVW010000052        10650 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_3999_length_10650_cov_8.400944, whole genome shotgun sequence.
ACCESSION   DAOOVW010000052 DAOOVW010000000
VERSION     DAOOVW010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10650
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3999_length_10650_cov_8.400944"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..1243)
                     /locus_tag="PLW95_06895"
     CDS             complement(80..1243)
                     /locus_tag="PLW95_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015842742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOV22385.1"
                     /translation="MKEIKIGLIGYKFMGKAHSHAYKDVGLFFQLSIRPIMKVICGRS
                     KKPLSIAKDRFGWEEYETDWRKVVTRDDIDMIDITTPPNAHKEIAIEAAKNGKIIFCE
                     KPLASRIEDAYKMVEVAEKYGVKHAICFNYRKLPAIGLAKKLIDEGKLGKIYHIRAVY
                     LQDWIIDPEFPLVWRLQKEIAGSGAHGDLNAHLIDMARYLVGEFDQVIGMNKTFIKQR
                     PLLEETEKLETGLKDKEKSKEKGIVDVDDATIFLAKFKNGVIGSFEATRFAAGRKNYQ
                     RFEINGSKGSIIFNLERLNELQYYSTDDPDYASGFRTIIATEPSHPYSANWWPAGHII
                     GYGESFINMVADIFNSIEKGENPSPNFYDGIKCQEVLEAVNRSIKEERWIELK"
     gene            complement(1230..2258)
                     /gene="recA"
                     /locus_tag="PLW95_06900"
     CDS             complement(1230..2258)
                     /gene="recA"
                     /locus_tag="PLW95_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOV22386.1"
                     /translation="MEKEKALELTISQIEKEFGKGSIMKLGEKTHIEVPVIPTGAISL
                     DIALGVGGIPRGRVTEIFGQESSGKTTLALSVLAQAQKAGGQVAFIDVEHALDPNYAK
                     KLGLKIEDMLVSQPDSGEQALEIAEALIRSNAIDVIVIDSVAALVPKAELEGEMGDSV
                     MGLQARLMSQALRKLTGYISKSNTSTIFINQVREKIGVFFGNPETTPGGRALKFYASV
                     RLELRRIESIKRGEDLIGNRVKVKVVKNKVAPPFKEVILDFYYGEGISKEASLIDAAI
                     SFGILEKKGSYFYYDGKTLEQGVDNMIKILKTNQKLYDEIENKVKEKSGFVVKKETKK
                     GEGKNERD"
     gene            complement(2263..2853)
                     /gene="thpR"
                     /locus_tag="PLW95_06905"
     CDS             complement(2263..2853)
                     /gene="thpR"
                     /locus_tag="PLW95_06905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOV22387.1"
                     /translation="MEVRGKFIRYFIKMRLFVGIKLPDNLKEEIRKIEASLRKKVREV
                     KVVSPENLHITLKFLGEVGEKDVGNIDEKLNEVKKKFSSFDVSIGKIGSFPDDKKIRV
                     LWIGVESHGKLKKLNSEIERQLVTIGYKEENRFTEHITIARFKSTPDLGFIEKLKEKY
                     NMVISSFKVENFSLINSKLTKSGPIYEDLETYKLGG"
     gene            complement(2899..3894)
                     /gene="ilvC"
                     /locus_tag="PLW95_06910"
     CDS             complement(2899..3894)
                     /gene="ilvC"
                     /locus_tag="PLW95_06910"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOV22388.1"
                     /translation="MAKIYYEKDADLGVFSGKKVGIIGYGSQGRAHALNLRDSGVDVV
                     VSELEGTPAYKKAVNDDFKVVDAAEVVKTCDVFMMLVQDNAQPTVYKNFIHPNLKKGQ
                     AMGFAHGFSIRFNQIVPPEDIDVFMVAPKGPGDLVREQFAQGQGVPCLVAVYQNPTGN
                     ALKIALAYAKGLGGTRCGVVETTFKEETETDLFGEQVVLCGGVTQLIKDAFEILVESG
                     YQPEVAYYETCHELKLIVDLINTRGIQGMRDVVSDTAEYGDMTRGPRVINECVREEMK
                     DILKEIQTGKFALEWILENQAGRPVYNSLKEEAREHLIEKVGAKMRSMMPWLKGK"
     gene            complement(3909..4418)
                     /gene="ilvN"
                     /locus_tag="PLW95_06915"
     CDS             complement(3909..4418)
                     /gene="ilvN"
                     /locus_tag="PLW95_06915"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOV22389.1"
                     /translation="MNNNGIKPHIISVTVENKFGVLSKIAGLFSARGYNIDSLSVAET
                     DDPTVSRMIIVVRGDEKILEQIYKQLNKLIDVIKVQDLTEQDFIERELALIKLSINNF
                     NERQEVLQLVQIFRAKIISVGKKTLTIEAVGDTSKLDALVDLLKPFGFKEFIRTGKIA
                     ISRETIEKK"
     gene            complement(4429..6096)
                     /gene="ilvB"
                     /locus_tag="PLW95_06920"
     CDS             complement(4429..6096)
                     /gene="ilvB"
                     /locus_tag="PLW95_06920"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOV22390.1"
                     /translation="MKGAKIFIECLKKEKVEVIFGYPGGTVLPFCDVLFDSGIRFILT
                     RHEQGAAHAADGYSRSSGKVGVCLATSGPGSTNLVTGIATAYMDSIPMVAITGQVATH
                     LIGNDAFQEVDTTGITRPITKHNYLIKDVNEICKVVKEAFYIATTGRPGPVLIDFPAN
                     IQSMEGKFNYPSKIEMRSYKPVYEGHPKQIEKAWELIESSKKPVIIAGGGIISSNASS
                     ELNEFVEKTGIPVAFTLMGLGCMDVRHPLSLGMPGMHGTKYANYAIVESDLIISIGCR
                     FDDRVTGKIENFAPKAKIIHIDIDPAAISKNVEVDVPIVGDAKNVLKKLLEEAKKLDI
                     EQWQEKVKKWKNENPLKYDNNGFKPQYVIEKISEITKGEAIIATEVGQNQMWTAQFYR
                     FKKPRTFITSGGLGTMGFGFPAAIGAKVANPYEIVIDIAGDGSIQMNIQELATAVSNK
                     IPVKIIILNNGYLGMVRQWQQLFYNRKYAFTCIANGQPDFVKLAESYGAIGYRVKTKE
                     EFDKVIDKVLEEKEKVVMLDCRIEPEENVFPMVPAGASLDQMLEGIA"
     gene            complement(6287..>6920)
                     /locus_tag="PLW95_06925"
     CDS             complement(6287..>6920)
                     /locus_tag="PLW95_06925"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOV22391.1"
                     /translation="IQNAKKLIESGKLGFIHRVVAFNCYFVDLIKESRSWKLNSKIEI
                     GSIFDTEVHYIDDLRYLLGKEIIEVYGKVRKFKKEFENAPADDVIAIIDFEEGSNGFL
                     EISENQWRGNGNISNGEGINFHGNDGSLEISLSGKFNAYFKDGQEIKENYTPDYNKIW
                     KEFHHSFVKSILEDSPIPVTGEEATKNLAVILAILKLSEKNRPISLIEDK"
     assembly_gap    6921..7020
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7255..9816)
                     /locus_tag="PLW95_06930"
     CDS             complement(7255..9816)
                     /locus_tag="PLW95_06930"
                     /inference="COORDINATES: protein motif:HMM:NF036482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22392.1"
                     /translation="MKKIDLKGKWMLLGCEPKTGLPADIIKPELEKEQWLIADVPGDV
                     NATLLKYNKIPNPHYDTQGRDCYWVTEKEWWYKLVFDASDVGNAKADLCLEGVDGHAD
                     IYLNNKYLGEMKNAFRQFRFNITGLLKLKKNVLLIRFKSIDQLLGGSRIDELLGWRER
                     RAFIRKPQFSFGWDWALPIPSIGLSGNVWIEYHNEFCIKEFSIQTFMNGRIDFSFEVS
                     KGTKLSGYSIIINIDGYGQNITKEIRRNTFKSYTSINIENPKLWFPNGYGEQPLYHYS
                     IGLLVSGNIVDKKEGYFGIRESKIIEEPFTPEAGHGFSFRITINGESIFCKGANWVPL
                     ELWPVNATEEQYRFYLNKAKEANFNMLRVWGGGIYEPEIFYKLCDELGIMVWQDFMFA
                     SSGYPVDLLRDEIIAEANYQIRRLRNHPCIVIWCGCNEDIYSWSYPEQEEDDTQQDSG
                     VYSKKESEDKWKVDRLKDDPQIYTMILRGLVSKFGLDVPYVDSSPSSKDDYGNMPNSG
                     NSHISCWKYSLFESDGKPEQFRKHFEKVCSFDSEFCIQGPCAEKTFKKFFSPDNLWPP
                     NQAWVYHIQRGHANLPHHEQTIFIAGAIFGKIDSLEKYVKFGQATHVEMMRAEFESAR
                     RDRPNNGGTMVWMFNDCWPTSNWSIIDYYRCPKPAFYAAKRACATLLPIIFERSEKIE
                     FFFSNDSSKNAEVELLYGQETLQGNQVWSKHKKLTIDKNSTKLFDAIERDKMVFPIGD
                     FLYIDAIVNNKKLPRVIYFPDGWKDIQWPHPNIEIKFIDQKFFKGKWNTTIKLRTDTF
                     ARFCHIILKENDSDISFSDNYFDMSASSDREINIQSDAKIDINNLIIGNWWTKWE"
     gene            complement(10305..>10650)
                     /locus_tag="PLW95_06935"
     CDS             complement(10305..>10650)
                     /locus_tag="PLW95_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22393.1"
                     /translation="PSQKFKYPSPRPVSYAFSLYTVTEGIKIRNDPVTRMAKLNRVKA
                     PSEKVWLSDYFRTYNFNPGLIVPTRWESEGFRIHNNGVNCLFFDGHVEWVSFERAQAP
                     VQGTPNPMFRPF"
BASE COUNT         3223 a         2124 c         1252 g         3951 t
ORIGIN      
        1 tcaaaaattt tatagttatt atatatttag atgacaaaat ttttgaaaag tttacttttg
       61 aatattttat tgttagttat tatttcaatt caatccatct ttcctctttt attgacctat
      121 taactgcttc aagaacttcc tggcatttta ttccatcata aaagtttgga gatggatttt
      181 cacctttttc aattgaattg aatatatctg caaccatatt tataaatgat tctccatatc
      241 caataatatg ccctgcgggc caccagttag cagaataagg atgggatggt tctgtggcaa
      301 ttattgttct aaaaccagaa gcatagtcag ggtcatctgt actataatat tgcaactcat
      361 taagacgctc taaattaaat ataatgcttc ctttactccc atttatttca aatctttgat
      421 agttttttct tcctgctgca aatctcgttg cttcaaaaga accaattact ccatttttaa
      481 actttgcaag aaaaattgtt gcatcatcaa catctacaat tcctttttct ttactttttt
      541 ctttatcttt aagccctgtt tctaattttt ctgtttcttc taatagtggt ctttgtttta
      601 taaatgtttt attcatacca ataacctggt caaattcacc aactaaatat cttgccatat
      661 caattaagtg tgcatttaaa tccccatgtg caccacttcc tgcaatttct ttttgaagtc
      721 gccatacaag aggaaattca gggtcaataa tccagtcctg aagataaaca gcccttatgt
      781 gataaatttt tcctaatttt ccttcatcta tcagtttttt tgccagccca atagcaggta
      841 attttctgta attaaaacaa attgcatgtt taactccata tttttcagca acttcaacca
      901 ttttatatgc atcctcaatt ctggatgcta atggtttttc acaaaatata attttcccat
      961 tttttgctgc ttcaattgct atttctttat gtgcatttgg tggcgtcgtt atatcgatca
     1021 tatcaatatc atctctcgta acaacttttc tccagtccgt ctcgtattct tcccatccaa
     1081 atctatcttt tgcaatagat aatggttttt tactccttcc acaaattact ttcataatag
     1141 gtctaataga aagttggaaa aaaagtccaa catctttata agcatgagag tgggctttcc
     1201 ccataaattt atatccaata agtccaattt taatctcttt catttttccc ctcccctttt
     1261 tttgtttctt ttttaacaac aaatccgctt ttttctttaa ccttattttc tatttcatca
     1321 taaagtttct ggtttgtttt taaaattttt atcatattat ctacaccttg ctctaatgtc
     1381 ttcccatcat aataaaaata acttcctttt ttctccaata tcccaaaact aatggctgca
     1441 tcaattaaag atgcttcttt gcttattcct tctccataat aaaaatctaa aataacttct
     1501 ttaaatggtg gagcaacttt attttttaca acttttactt ttacccggtt tcctataagg
     1561 tcctcacctc ttttaatact ttcaattctt cttaattcaa gacggactga agcatagaat
     1621 tttaaagccc ttcctccagg tgttgtttct ggatttccaa aaaaaacacc tattttttct
     1681 cttacttgat ttataaaaat tgttgatgta tttgatttgc ttatataacc agtcaatttt
     1741 cttaatgcct gagacattaa ccttgcctgt aatcccataa cactatctcc catctctcct
     1801 tctaattctg cttttgggac aagagcagca actgaatcaa tgacaataac atcaattgca
     1861 ttacttctta taagtgcttc tgctatttcc aatgcctgtt cacctgagtc aggttgactg
     1921 acaagcatat cttcaatttt caaacccaat ttttttgcat aatttgggtc caaggcatgt
     1981 tcaacatcaa taaaagcaac ctgcccccct gctttttgtg cctgtgctaa tacacttaaa
     2041 gcaagagtgg tttttccaga agattcctgt ccaaaaattt ctgttaccct tcctcttgga
     2101 ataccaccaa caccaagtgc aatatctaag gaaattgctc ctgttggtat tacaggaact
     2161 tcaatatgcg ttttttctcc taatttcatt attgaacctt ttccaaattc tttttctatc
     2221 tgagatattg taagttcaag tgctttttcc ttttccattt ttttatcctc caagtttata
     2281 ggtctctaaa tcctcataaa ttggaccact ttttgttaat ttactattta taagagaaaa
     2341 gttttcaact ttaaaactac ttatgaccat attatacttt tcttttaatt tttcaataaa
     2401 tcctaaatcg ggtgtggact taaatcttgc tattgtaata tgttctgtaa atctattttc
     2461 ttctttatat ccaatagtta caagttgcct ctcaatttct gaatttaact ttttaagttt
     2521 cccatgactt tcaactccaa tccataagac cctgattttt ttatcatctg gaaaacttcc
     2581 tattttccct attgagacat caaaggaact aaatttcttt ttaacttcgt ttaatttctc
     2641 atcaatattt ccaacatctt tttcgcctac ttctccaaga aactttaatg taatatgcaa
     2701 attttcaggt gatacaactt taacttctct aacttttttc cttaaagatg cttcaatttt
     2761 tcttatttct tcttttaaat tatcaggtaa ttttattcca acaaatagtc gcattttaat
     2821 aaaatatcta ataaattttc ccctcacctc catcctctcc ccttagggga gaggaaagat
     2881 gggatatttg actggtattt atttaccttt tagccagggc atcatacttc tcatttttgc
     2941 ccccactttc tcaattaaat gttctcttgc ctcttctttt agtgaattat aaacaggtct
     3001 acctgcttga ttttcaagta tccattccag agcaaattta cctgtctgta tttcttttaa
     3061 aatatctttc atctcttctc taacacactc atttataact cttggacccc ttgtcatatc
     3121 accatattca gcagtatcac tcacaacatc tctcattccc tgaattcctc ttgtatttat
     3181 taaatcaaca attagtttca attcgtgaca tgtttcataa tatgcaactt ctggttgata
     3241 tccactttca acaagtattt caaaagcatc ttttataagt tgagttactc caccacaaag
     3301 aacaacctgc tcaccaaaaa ggtctgtttc tgtttcttct ttaaatgttg tttcaactac
     3361 tccacatctt gttcctccta aaccctttgc atatgcaagt gcaattttaa gggcatttcc
     3421 agttggattt tgataaacag ccactaaaca tggaactccc tgaccttgag caaactgttc
     3481 cctaacaagg tctccaggac cttttggcgc gaccataaaa acatctatat cttctggtgg
     3541 gacaatttga ttaaaacgta ttgaaaaacc atgtgcaaac cccattgcct gccctttttt
     3601 taaatttgga tgaataaaat ttttataaac agttggctgt gcattatcct gaacaagcat
     3661 cataaaaaca tcacatgttt taacaacttc tgctgcatca actactttaa aatcatcatt
     3721 tactgccttt ttataggcag gggttccttc taactcagaa acaacaacat caaccccgct
     3781 atctcttaaa tttagagcat gtgcccttcc ctgactacca tatcctataa ttccaacctt
     3841 tttaccacta aaaacaccta aatcagcatc tttctcataa taaatctttg ccatttcttc
     3901 ctcctttttt actttttttc aatagtttct cttgatatcg caattttacc tgttctgata
     3961 aactctttaa aaccaaatgg tttaagaagg tcaaccagag catctaattt tgaagtatct
     4021 cctaccgctt caatagttag tgttttcttt cctacactta ttatttttgc tctaaaaatt
     4081 tggacaagtt gcaaaacctc ctgcctctca ttaaaattgt taattgataa cttaataaga
     4141 gcaagctctc tctcaataaa atcctgttct gttaggtcct gaacttttat aacatctatg
     4201 agtttattta attgtttata tatctgttca agtatttttt catcccccct aacaacaatt
     4261 atcattcttg aaacagttgg gtcatctgtc tctgcaactg agagggaatc aatattataa
     4321 ccccttgcag aaaaaagtcc tgcaattttt gacagaactc caaatttatt ttcaacagtt
     4381 actgaaataa tatgcggttt tatgccgtta ttattcattt tttctccctt atgctattcc
     4441 ttcaagcatc tggtccaatg aagcacctgc aggtaccatt ggaaaaacat tttcttctgg
     4501 ttcaattcta caatcaagca taacgacttt ttctttctcc tccaaaacct tatctattac
     4561 tttatcaaat tcctcttttg tttttactct atatccaatt gccccataac tttctgctaa
     4621 tttaacaaaa tcaggctgac catttgcaat acaggtaaaa gcatattttc tattataaaa
     4681 caattgttgc cattgtctca ccattcctaa atacccattg ttaagaataa taatttttac
     4741 aggaatttta tttgaaactg ctgttgcaag ttcctgaata ttcatctgta tacttccatc
     4801 ccctgcaata tcaataacaa tttcatatgg atttgcgact tttgcaccaa ttgcagcagg
     4861 aaaaccaaat cccattgttc caagtccacc tgatgtaata aaagttcttg gctttttaaa
     4921 tctataaaat tgtgctgtcc acatttgatt ttgtccaact tctgttgcaa ttattgcctc
     4981 tccttttgta atctctgata ttttttctat tacatattgt ggtttaaatc cattgttatc
     5041 atatttaaga gggttctcat ttttccattt tttgactttc tcctgccatt gttcaatatc
     5101 caattttttt gcctcttcta ataatttttt taaaacattt tttgcatcac caactattgg
     5161 aacatctact tcaacatttt tacttattgc agctgggtca atatcaatgt gaataatttt
     5221 tgcttttgga gcaaaattct ctattttccc tgtcactctg tcatcaaacc tgcatccaat
     5281 agaaataatt aaatcacttt caacaattgc ataatttgca tattttgttc catgcatacc
     5341 aggcataccc aatgaaagag gatgccttac atccatacaa ccaagaccca taagagtaaa
     5401 ggcaactgga atccctgttt tttcaacaaa ttcattcaat tcactacttg cgttagaact
     5461 tataattcct ccacctgcta ttattactgg tttttttgaa ctttcaatta actcccatgc
     5521 tttctcaatt tgttttggat gtccttcata aacgggctta taacttctca tttcaatttt
     5581 tgatggataa ttgaattttc cttccatact ctgtatatta gcaggaaaat caattaaaac
     5641 aggtccaggt ctacctgttg ttgcaatata aaatgcctct ttcacaactt tgcatatctc
     5701 atttacatct tttattaaat aattatgttt tgttattggc cttgttattc cagtagtatc
     5761 aacttcctga aaagcatcat ttccaattaa atgagttgca acctgtcctg taattgcaac
     5821 cattggtatt gaatccatat aagcagttgc aattcctgta acaagatttg ttgacccggg
     5881 accagaagta gcaagacaaa ccccaacttt tccagatgac cttgaataac catcagcagc
     5941 atgagcagca ccctgttcat gccttgttaa aataaatctt atcccactat caaaaagaac
     6001 atcacaaaat ggtaaaactg ttccacctgg ataaccaaat ataacttcaa ctttttcttt
     6061 tttcaaacat tctataaata tctttgcacc tttcattttt ctctccattc attatttaac
     6121 ttatgaaata ttatatcaca aaaaaaacaa aatcaaattt gacaacatag agtttgacca
     6181 gatacaaaga aggttggtct gtattcttga accctgtatt gataaatgtc ctgtaaaagg
     6241 tattaaaaaa taaaattgcc ttttgtatat tacaaaagaa attaagttat ttgtcttcaa
     6301 taagagaaat aggtctattt ttttctgata attttaaaat tgctaaaata actgcaaggt
     6361 tttttgttgc ttcttcacca gtaactggaa ttggtgagtc ctctaaaata ctttttacaa
     6421 atgaatggtg aaattctttc cagattttat tataatcagg agtataattt tcttttattt
     6481 cctgtccatc tttaaaataa gcattaaatt tccctgaaag tgaaatttca agagaaccat
     6541 catttccatg aaaatttata ccttcaccat ttgaaatatt cccattacct cgccactgat
     6601 tttcacttat ttccaaaaaa ccattacttc cttcttcaaa atcaattatt gctattacat
     6661 catcagcagg agcattttca aactcctttt tgaattttct tactttccca taaacctcaa
     6721 ttatctcttt cccaagaaga tacctaaggt catcaatata atggacctct gtatcaaaaa
     6781 ttgaaccaat ttctatttta ctattaagtt tccaactgcg actttctttt ataaggtcaa
     6841 caaaataaca attaaaagca acaaccctat gaataaaacc aagttttcca ctttcaataa
     6901 gttttttagc attttggata nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 ccaaattcct gtgcaaagtt ttctgcttgt gaaatatcaa catcacataa tgtttttaat
     7081 tctgtctctt ctaatgattt tattgcctct atgtgacaac gactaagata accacaccca
     7141 atcatcccat attttcaatc tttttcccat agttttcctt tcttttgtaa aatttttctt
     7201 gtttttttat tatgcaggag attgctcttt ttataggaat ttttggtatt tactttattc
     7261 ccattttgtc caccaattgc caattattaa attattaatg tcaatttttg cgtctgactg
     7321 aatattgatt tctctatcac tactggctga catatcaaaa taattatctg aaaaagatat
     7381 atctgaatca ttttctttta atataatatg acaaaaacga gcaaatgtat cagtccgaag
     7441 tttaatagta gtattccact ttcctttaaa aaatttttga tcaataaatt taatctcaat
     7501 attaggatgt ggccattgaa tatctttcca tccatcagga aaataaatta ccctaggcag
     7561 ttttttatta ttaacaatag catcaatata taaaaaatct cctattggaa ataccatttt
     7621 atctctttca atagcatcaa ataatttagt tgaatttttg tctattgtta atttcttatg
     7681 ttttgaccaa acttgattac cttgtaatgt ttcctgtcca taaagtaatt ctacttctgc
     7741 atttttagat gaatcgttgc taaaaaagaa ttcaattttt tcagagcgtt caaaaattat
     7801 tggaagtaat gtagcacatg ctcgtttagc agcataaaat gcaggttttg gacatctata
     7861 ataatcaata attgaccagt tgcttgtagg ccagcaatca ttaaacatcc ataccatcgt
     7921 tcctccatta tttgggcggt ctcgtctagc actttcaaat tcagccctca tcatttctac
     7981 atgagttgct tgaccaaatt tcacatattt ctcaagagaa tctattttcc caaaaatagc
     8041 acctgcaata aaaattgttt gttcatgatg gggtaaattt gcatgacctc gttgtatatg
     8101 atatacccac gcttgattag ggggccagag attatcagga gaaaaaaact ttttaaatgt
     8161 tttttctgca caaggacctt gaatacaaaa ttctgaatca aaagaacata ctttttcaaa
     8221 atgtttccta aattgttctg gtttcccatc tgattcaaac aaagaatatt tccagcaaga
     8281 tatatgcgaa tttcctgaat taggcatatt gccataatca tcctttgaag atggacttga
     8341 atctacataa ggaacatcaa gaccaaattt actgactaat ccccgtaaaa tcatagtata
     8401 tatttgtggg tcatctttta atctatcaac tttccattta tcttcacttt cttttttaga
     8461 ataaacacca ctatcttgct gagtatcatc ctcttcctgt tcaggataac tccatgaata
     8521 aatatcttca ttacagccgc accagattac aatacatgga tgatttctaa gacgtctgat
     8581 ttgataattt gcttctgcaa taatttcatc ccttaataga tcaacaggat aaccagaact
     8641 cgcaaacata aaatcctgcc agaccattat tccaagttcg tcacataatt tataaaaaat
     8701 ttctggttca tatattccgc caccccaaac gcgaagcata ttgaaatttg cctcttttgc
     8761 tttatttaag taaaacctat attgttcttc tgtggcattt actggccata attctaatgg
     8821 aacccaattt gcccctttac aaaatataga ttctccatta attgtaattc taaatgaaaa
     8881 accatgtcct gcttcaggag taaatggttc ttctataatt tttgattcgc gaataccaaa
     8941 atacccttct tttttatcta caatattacc agaaactaaa aggcctattg aataatgata
     9001 tagtggttgt tctccatatc cattaggaaa ccataatttt ggattttcaa tatttatact
     9061 tgtataagat ttaaaagtgt ttcttctaat ttcttttgta atattttgac catatccatc
     9121 aatatttatt ataatactat aacctgatag tttcgttcct ttactcactt caaatgaaaa
     9181 atctattctg ccattcataa atgtttgaat agaaaattcc tttatacaaa actcattatg
     9241 atattctatc catacattcc ctgaaagacc tattgaagga ataggtagtg cccaatccca
     9301 gccaaatgaa aattggggct tacgaataaa agcccttctt tctctccatc ctaagagttc
     9361 atcaatacgg gaaccaccta aaagttggtc aattgatttg aatctaatta acaaaacatt
     9421 tttttttaat tttaataaac cagttatatt aaaacgaaat tgacgaaaag catttttcat
     9481 ctcaccaaga tacttattat taaggtaaat atctgcatgt ccatctactc cttctaaaca
     9541 aaggtctgct tttgcattac ctacatctga agcatcaaat actaatttat accaccattc
     9601 tttttctgta acccaataac aatctcgtcc ctgagtatca tagtgagggt taggaatttt
     9661 gttatacttt aataaggtag cattaacatc tcctggaaca tcggctatta accactgttc
     9721 tttctctaat tctggtttaa taatatcggc aggtagtcct gttttaggtt cacaacccaa
     9781 taacatccat ttccccttta agtcaatttt tttcataata tttccctgta agtattcagt
     9841 gaatcccatc ccgataattc gggccctttt ctgtctttgc acggtactaa acacagaatg
     9901 agatttactt tcaacaaatc aatttttctt ttaactttat cacatacatc tatgaattga
     9961 caaattttaa ttctgttttc attatactcc ccccaagttt tttcaattgt tctgcaggtc
    10021 cttaatatat gtagattttg atttattttc aaaaaaacaa aaagagacat tgataggtat
    10081 cagtgtattg acaaacagaa agaagaaaaa cttaaactct aagaattttt accaaataaa
    10141 cttgatcaga aaataaaaac tcttttaatt ctaaaaaccc tcttaaaata acttcttcaa
    10201 gtttagccta ggtatattat tgagcaataa gatatcgtag atgctaaaaa ttaggactgc
    10261 tctaatggag ttcagttagg attgacctca ttttttactc cctgttaaaa aggtcgaaac
    10321 attggatttg gcgtaccttg tacaggagct tgtgcccttt caaaacttac ccattctaca
    10381 tgcccatcaa aaaaaagaca attcactcca ttattatgta tcctaaaacc ttccgattcc
    10441 caccgtgttg gcactattag accaggatta aagttatagg tcctaaaata atctgataac
    10501 catacttttt cagatggcgc ttttactctg ttaagttttg ccattcttgt tactggatcg
    10561 tttctgattt ttattccctc agttactgta tatagagaaa aagcataaga aactggacga
    10621 ggagagggat atttaaattt ctggcttgga
//
LOCUS       DAOOVW010000053        10539 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_4055_length_10539_cov_9.100248, whole genome shotgun sequence.
ACCESSION   DAOOVW010000053 DAOOVW010000000
VERSION     DAOOVW010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10539
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4055_length_10539_cov_9.100248"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..413)
                     /locus_tag="PLW95_06940"
     CDS             complement(153..413)
                     /locus_tag="PLW95_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22394.1"
                     /translation="MEKNKTVDRKRKINEKKFGKWDTFPDGGRKYWMEIRGKSGWSAR
                     YIKEVDKNENTIKFYQEIYNGKGELVEIHCKFPKDTGHKRIR"
     gene            complement(416..676)
                     /locus_tag="PLW95_06945"
     CDS             complement(416..676)
                     /locus_tag="PLW95_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22395.1"
                     /translation="MKVNRKIVAEKLKDYLDFKISHSQLVDWAENIIEKGEFDENDVE
                     ILREIVGYIGLSDVKEFGLSWEDCKNFLEKLGFKVKVEVIIN"
     gene            complement(841..912)
                     /locus_tag="PLW95_06950"
     tRNA            complement(841..912)
                     /locus_tag="PLW95_06950"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(878..880),aa:Phe,seq:gaa)
     gene            1077..2477
                     /locus_tag="PLW95_06955"
     CDS             1077..2477
                     /locus_tag="PLW95_06955"
                     /inference="COORDINATES: protein motif:HMM:NF015442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HOV22396.1"
                     /translation="MEEIKNVRKRDGRIVPFDKEKITEAIFKAAKSVGGENRYLAEDI
                     TEAVTLYLNKKFQGQTPSVEEIQDAVERVLIKTGHAKTAKAYILYRQRRANVRKVREG
                     IKPPDLIEREEIRNIRDIKISVVRSDENLTDWNREKIIEALLRETGISRNIAEIIVSE
                     VEEEVITSKLNVLTSSIIRELVNSKLVKYGFEKEREKHSRIGIPLYDVEQIFKNFKGT
                     PDQLSLKFGQHIKKEYAISNVFSDEVIFSHFKKEIFIHCLEEIDKFYSLEVNWRDEEE
                     NLKRAMDWCSFVFFKSDKLLNWQGSNNNILRAVEIPLFGGFDFLAEIVKERPFEVELN
                     KERQGDILYVGKISLNTPSIVSKDKIFSIIKKAFDEKRKFLENILSDEIKEFFDSFKK
                     KNYLEISTEDINIENFIFPDEDIKIGLKTSSENFVNYLKRIESKNFAIKGKISQNNIS
                     ILHEMKEDIFVKVNLW"
     gene            2471..3361
                     /locus_tag="PLW95_06960"
     CDS             2471..3361
                     /locus_tag="PLW95_06960"
                     /inference="COORDINATES: protein motif:HMM:NF017847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HOV22397.1"
                     /translation="MVIKVGLFGYQGSGKTTLFKKYTGKEEENYDPFVPKIGIGRYED
                     ERLKKIKEVLNPEKVIFPEFEFYDFKGFPEGEGFPPEFFKNFYTLDIIICVVKNFSDD
                     AKPEEEANSLIMELIFHDIDKLEGIKQTRDKEGNIEENKIIEKALEILQKDKLLKSLQ
                     ENDKRQLSGIQLLTTKDFLIYLNGNKKKVNLSYSVVSEEEDIYKKIIEQLSMITFYTI
                     KGDIAQGWIIPENLSAKQAAGKIHKDIEKGFIKAAVLPWNNFVEIGDWKKAKNMGVLK
                     FLGPNSIFSDGDVVEFYFSK"
     gene            3526..4665
                     /locus_tag="PLW95_06965"
     CDS             3526..4665
                     /locus_tag="PLW95_06965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOV22398.1"
                     /translation="MEDIKNKVKKIEKDIISWYKDLHRYPELGFQEFKTSKKIQSLLK
                     KFKIPYQVKATTGVVGYIKGKRDKTLGIRADIDALPIEEKTPIPFKSKHKGCMHACGH
                     DGHTAILLGVIKVLSELKELKSNIKFIFQPSEERPPSGAKQMIEEGVIDDVDCIIGFH
                     LYSVIPSRKLRIGKGPVSANTDLFKIKIKGKGGHGSRPHLTNDPITCTGYLITFLQTI
                     VSRKIDPIAPCVVSIGQISSGSVFNVIPDDVEIVGTVRTLSEDIRDKIKEEMEKITEK
                     VCSSFGCKGKVEYNKHLPANVNDAFLTEKIINITQKYYPESLSESRLSMGGEDFAFFA
                     QKIPSTYIYIGMGEKCGPIHSSIFKIDEKILPFAVEYLTTLLVNL"
     gene            4829..5896
                     /locus_tag="PLW95_06970"
     CDS             4829..5896
                     /locus_tag="PLW95_06970"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOV22399.1"
                     /translation="MEYRQLGRSGLRVSKLCLGTMRDFTESNYDEAKKVVDEAIDSGV
                     NFIDTADCYGQSEEILGRIFAENGKREKVVLATKFGWYMGEGANDYGSGRKHIIESCE
                     GSLKRLKTDWIDLYIIHVIDPNPPMDETLYALDTLVRQGKVRYIGTSKHPASIILEGL
                     FISEKYGWAKFVSEQPPYHILDRSIENELIPMCVKHGVGITPFWPIASGLLSGKYRSG
                     KETTEGRFARGKVDEKILEVIEKLIPLAENRGITLAEFSLAWLMQQPGVTAPILGARK
                     VEYIRSGVKACDIKLTEEEINKVDEIVPPGTYIVPHYENNIWRPARMFYSSKARSIKG
                     TGAYIPDTKTDSSKKSGYPQL"
     gene            complement(6021..6488)
                     /gene="ispF"
                     /locus_tag="PLW95_06975"
     CDS             complement(6021..6488)
                     /gene="ispF"
                     /locus_tag="PLW95_06975"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOV22400.1"
                     /translation="MKIGIGFDIHRFEKDRKFILGSVEIPYSHGLIGHSDGDVLLHSL
                     SDAILGSLAKPDIGFYFPDTDEKIKGIDSKKILKKVLTIMKKENCKIENIDVVIICEK
                     PKLSPYVPLIRKKISEITGIDEKNIGIKVKTYEGIGEIGKGNACACLSSVLIS"
     gene            complement(6569..7573)
                     /locus_tag="PLW95_06980"
                     /pseudo
     CDS             complement(6569..7573)
                     /locus_tag="PLW95_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546645.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            complement(7608..7693)
                     /locus_tag="PLW95_06985"
     tRNA            complement(7608..7693)
                     /locus_tag="PLW95_06985"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7657..7659),aa:Ser,seq:gga)
     gene            complement(7679..7891)
                     /locus_tag="PLW95_06990"
     CDS             complement(7679..7891)
                     /locus_tag="PLW95_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22401.1"
                     /translation="MGKYSTDVRKIYKKKQIKAKEKLKKLETGEISYSQLNRLSKKLF
                     YKRLKAGYEFPARIFSSDKKSSGGVA"
     gene            complement(8045..8206)
                     /locus_tag="PLW95_06995"
     CDS             complement(8045..8206)
                     /locus_tag="PLW95_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22402.1"
                     /translation="MSEVPRWFPMSLNFKNISFKNEYLRSSLIAEIHCLFALLPLVFK
                     EIISFWIKL"
     gene            9369..9734
                     /locus_tag="PLW95_07000"
     CDS             9369..9734
                     /locus_tag="PLW95_07000"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOV22403.1"
                     /translation="MGNYICIFYKTVSGNSPVEDFIESLDEDTQDKFIYKKELLEHFG
                     PQLRCPHTSEISEGIFELRFKGKEGQIRILFFFYYEKQIILVHGFVKKTQKTPKKEIN
                     IAKKRKTEYYQRVKEEEKL"
     gene            9731..10048
                     /locus_tag="PLW95_07005"
     CDS             9731..10048
                     /locus_tag="PLW95_07005"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOV22404.1"
                     /translation="MRKVDDYIKEKMTKNPDFKSRYEIIKQKVEVAKRIIEYRNKYNL
                     SQSQLAKKLGVSQQYISKIEEGEFSNIETVENILNQIGYCLKLEVVPLSNQDKGSYQQ
                     VLP"
BASE COUNT         3909 a         1480 c         1730 g         3420 t
ORIGIN      
        1 agggggattg ttctacacag gcagtttaag gaaagcatac gaaccagccc taccctcctt
       61 cgctaatgct cagacgggca ggcgcagaag tgctaagttg ggcttacggg gtttccaagc
      121 acaacgggta aagaggggga tttgtattgc ttttatctaa ttcttttatg tcctgtatct
      181 tttgggaatt tacagtggat ttccaccaat tcccctttcc cattataaat ttcctgataa
      241 aactttattg tattttcatt tttatctact tctttaatat atcttgcact ccaccctgat
      301 ttacctctta tttccatcca atattttctt cctccgtcgg gaaaagtatc ccacttccca
      361 aatttcttct catttatctt tcttttccta tctactgttt tatttttttc cacagttaat
      421 taattataac ttcaactttt actttaaacc ccaatttttc aagaaaattc ttacaatctt
      481 cccatgatag cccaaattct ttaacatcag aaagcccgat ataacctact atctctctta
      541 aaatttctac atcattttca tcaaactcgc ccttttcaat tatattctct gcccaatcca
      601 caagttgaga atgagatatt ttaaaatcta aataatcttt taatttttct gctacaattt
      661 ttctgtttac tttcattatt ttgctctcct ttttttcaat tcacattata tttattaaat
      721 tccctactcc cctttgaaaa atagacaaaa gcaggggata gcaagcacaa cagggaatga
      781 tgcttttcct ttttacctgt tgctttcagt caactacgtt ggctgattat ttatagaagg
      841 tgccggggga cagaatcgaa ctgtccacgc cagccttttc agggctgcgc tctaccactg
      901 agctaccccg gcatgataaa tattaaagaa gtattgctat actcatgctt aaaataaaac
      961 tacaccaacc tgataaactt cactaaagtc ataaacttaa aataccacaa atataactat
     1021 tttgtcaagg aaacgaaaag tttgtcatta aaggaaagaa aaagtataat gaaaaaatgg
     1081 aagaaataaa aaatgtcaga aaaagagatg gcagaattgt tccttttgat aaagaaaaaa
     1141 taacagaagc aatatttaaa gcagcaaaaa gtgtaggggg agaaaacagg tatttggcag
     1201 aagatattac agaagcagtt actctttatc ttaataagaa atttcaggga caaactccat
     1261 ctgttgaaga aattcaagat gcagttgaaa gagttcttat aaaaacaggg catgcaaaaa
     1321 cagccaaagc atatatactt tatagacaaa gaagagcaaa tgtaagaaaa gtaagagaag
     1381 gaataaaacc acctgattta attgaaagag aagaaataag aaatataaga gatataaaaa
     1441 tatctgttgt tagaagtgat gagaatttaa ctgactggaa tagagaaaaa ataatagaag
     1501 cacttctgcg agaaacaggt atatcaagaa atattgcaga aattattgta agtgaagtag
     1561 aagaggaagt tattacttca aaactaaatg ttttaacaag ttcaattata agagaacttg
     1621 taaatagcaa gttagtaaag tatggttttg aaaaagaaag agaaaaacat tctcgtattg
     1681 ggatacctct ttatgatgta gagcaaattt ttaagaattt taaagggacc ccagaccaac
     1741 tatctttgaa attcgggcaa catattaaaa aagaatatgc aatttctaat gttttttctg
     1801 atgaagtaat ttttagtcat tttaaaaaag aaatttttat acattgttta gaagaaatag
     1861 ataaatttta tagtttagaa gttaattgga gagatgaaga agaaaattta aaaagagcaa
     1921 tggattggtg ttcctttgtt tttttcaagt cagataagtt attaaattgg cagggaagta
     1981 ataataatat tttaagggca gttgaaatac ctctttttgg tggatttgat tttcttgcag
     2041 aaattgtcaa agaaagacct tttgaagtag aattaaacaa agaaaggcag ggagatatcc
     2101 tttatgtagg gaaaataagt ttaaatacac cttcaatagt aagtaaagat aaaattttca
     2161 gtataattaa aaaagcattt gatgaaaaaa ggaaattttt agagaatatt ttaagtgatg
     2221 aaattaagga attttttgat agttttaaga aaaagaacta ccttgaaata agtacagaag
     2281 atataaatat tgagaatttt attttccctg atgaagatat aaaaattgga cttaaaacaa
     2341 gttctgaaaa ttttgttaat tatctaaaaa gaatagaaag taaaaatttt gcaattaagg
     2401 gtaaaataag tcaaaataat attagtattt tgcatgaaat gaaagaagat atttttgtaa
     2461 aggtgaattt atggtaatta aagttggact ttttgggtat cagggaagtg gaaaaacaac
     2521 tttattcaag aaatatacag gtaaagaaga agaaaattat gacccatttg ttccaaaaat
     2581 tggaattgga agatatgaag atgaacgatt gaaaaaaata aaagaggttc tcaatccaga
     2641 aaaagttata tttcctgaat ttgaatttta tgattttaaa ggttttccag aaggtgaagg
     2701 ttttccacca gaatttttta aaaattttta tactcttgat ataataattt gtgttgttaa
     2761 aaatttttct gatgatgcaa aaccagagga agaggcaaat tctttgataa tggaacttat
     2821 ttttcatgat attgataaat tagaaggaat aaaacaaaca agagataaag aaggcaatat
     2881 agaagaaaat aaaattatag aaaaggcatt agagatatta caaaaagata aattattaaa
     2941 gtcattacaa gaaaatgaca aaagacaact ttcgggaatt caacttctta caacaaagga
     3001 ctttcttata tatttaaatg gaaataagaa aaaagttaac ctctcatatt ctgttgtgag
     3061 tgaagaagaa gacatttata aaaaaattat agagcaactt tcaatgataa ctttttatac
     3121 aattaaaggg gatattgcac agggatggat aattcctgaa aatttatctg caaaacaggc
     3181 agcaggtaaa attcataagg atattgaaaa agggtttata aaagcagctg tcttgccatg
     3241 gaataatttt gttgaaatcg gtgattggaa aaaagcaaaa aatatgggtg ttttaaaatt
     3301 tcttggacca aactcaattt tttctgatgg agatgttgtt gaattttatt tttcaaagta
     3361 ataatataat cagttaactg cgttgactaa gaggtagcaa gtataaatga aaaattaaaa
     3421 acgcacatta ctaaaacttt gtgcttctgt gctttactca cgggtttact gaatatttaa
     3481 tatttacttt aaatgtctat aatggaaaaa ggatgaatat tcaaaatgga ggacataaaa
     3541 aataaggtaa aaaaaataga aaaagatatt attagttggt ataaagattt acatcgatat
     3601 cctgaacttg gatttcagga atttaaaaca tcaaaaaaaa tacaatctct tttaaaaaaa
     3661 tttaaaatac cataccaggt aaaggcaaca acaggagttg taggatatat caaaggcaaa
     3721 agagacaaaa cacttggaat aagggcagat atagatgctt taccaattga agaaaaaaca
     3781 cctattccgt ttaaatcaaa acataaaggg tgtatgcatg cttgcggtca tgatggacat
     3841 acggcaatac ttcttggggt aataaaggtt ttatctgagt taaaagaact aaaatcaaac
     3901 atcaaattta ttttccagcc gagcgaagaa agaccaccaa gtggtgcgaa acaaatgatt
     3961 gaagaaggtg ttattgatga tgtagattgt ataattggtt ttcatctgta ttcggtgatc
     4021 ccatctagaa aattacggat tggcaaaggt cctgtttcag caaacactga cctgtttaaa
     4081 ataaaaataa agggaaaagg gggacatggt tctcgtccac atttaacaaa tgacccgatt
     4141 acctgtactg gttatcttat tactttttta cagaccattg tgagtagaaa aatagacccc
     4201 attgctccct gtgttgtttc aattggacaa atttcaagtg ggtctgtatt taatgtaatt
     4261 cccgatgatg ttgaaattgt agggactgta agaacattat cagaagacat aagagataaa
     4321 ataaaggaag aaatggaaaa aataacagaa aaagtttgtt cctcttttgg ttgtaaagga
     4381 aaagttgaat ataataaaca tcttcctgct aatgtaaatg atgcatttct tactgaaaag
     4441 ataataaaca ttacacaaaa atattatcca gaaagtttat ctgaatctcg tctctccatg
     4501 ggaggagagg actttgcttt ttttgctcaa aaaataccat caacatatat ttatattgga
     4561 atgggagaaa aatgtggacc tattcatagt tctatattta aaatagatga aaaaatttta
     4621 ccttttgcag ttgaatattt aactacttta ttagttaatt tataaacaat cagtcaacac
     4681 aattgcttga gaagtagcaa gtagtaagat aaaggaaagg atttaacctt atatgtcttg
     4741 ctttaaaaaa gagttagcgg gctttttctt tctaatataa tggtaaggat gcttagatga
     4801 atatttatgt gtcgaaggag gggaagaaat ggaatataga cagttaggaa gaagtggatt
     4861 aagagtttca aaattatgtt taggaacaat gcgtgatttt acagaaagca attatgatga
     4921 agcaaaaaag gttgttgatg aggcaattga ttcaggtgtt aactttattg atactgctga
     4981 ttgttatggg caatcagaag aaattttagg gaggattttt gctgaaaatg gaaaaagaga
     5041 aaaagtagtt cttgcaacaa agtttggatg gtacatggga gaaggtgcaa atgattatgg
     5101 ttcaggaaga aagcatataa ttgagtcctg tgaaggtagt ttaaaacgac taaaaaccga
     5161 ctggatagac ctttacataa ttcatgttat agacccaaat cccccgatgg atgagacact
     5221 ttatgctttg gatacacttg taagacaggg aaaagtaaga tacataggaa cttcaaaaca
     5281 tcctgcaagt attatacttg aagggctctt tataagcgag aaatatggat gggcaaaatt
     5341 tgtaagtgag caacccccct accatatcct tgatagaagt attgaaaatg aacttattcc
     5401 tatgtgtgta aaacatggtg ttggaattac tccattctgg cctattgcgt caggcctttt
     5461 atcaggaaaa tatcgttctg gaaaagaaac aacagaaggc cgttttgcaa gagggaaagt
     5521 agatgaaaaa attttagaag taatagaaaa acttattcct cttgcagaaa atagaggaat
     5581 tacattagca gaattttctc ttgcatggct tatgcaacaa cccggtgtaa cagcacctat
     5641 tcttggagca agaaaagttg aatatataag gtctggagta aaagcgtgtg atatcaaatt
     5701 gacagaagaa gaaattaaca aagttgatga gatagtacct cctggtactt atattgttcc
     5761 tcattatgaa aataatatct ggagaccagc aaggatgttt tattcatcaa aagcaagaag
     5821 tataaaagga acaggtgcat atattcctga tacaaaaaca gattcttcaa aaaaatcagg
     5881 ttatcctcaa ttgtgaaaga cataaaaggg aaaaaagaaa taacaggggt cagaaagtag
     5941 cgagtagata aaaaaaagta aagaacgcag ataaaaaagt gaataactaa tagggcataa
     6001 ttttaacagg aataagaaat ttaagaaatt aaaacagacg aaagacaggc acatgcattt
     6061 ccttttccta tttcaccaat tccttcgtat gttttaactt ttatccctat atttttttca
     6121 tcaatccctg taatttcaga aatttttttt cttattaagg ggacataagg agaaagtttt
     6181 ggtttttcac atattataac aacatcaata ttttctattt tacaattttc ctttttcatt
     6241 attgtaagaa ctttttttag aattttttta ctatcaattc cttttatttt ttcatctgta
     6301 tcaggaaaat aaaaaccaat atctggttta gccagagaac ctaaaatagc atcagataaa
     6361 gaatgaagaa gaacatctcc atcactatgc ccgataagtc catgactata aggtatttca
     6421 acactcccaa gaataaattt tctgtccttt tcaaatctat gaatatcaaa cccaatccct
     6481 attttcatat tatttttacc tcacaagaaa ttatttaaaa aattttttat aatttttcca
     6541 atctctcaat ctcttgtttt atttcttcaa ttacttctgg actaataata gtttttactg
     6601 aaagtaattc tctttttgtt gaatcagata ggtcaatttt tgaaccgaat tttaattttt
     6661 ttgggtctat tttcttatca aattttatat ggtctttaaa gatataaggt aaatttgaaa
     6721 tctctaaatt ccaatttgtg tttctaaatt tttgctttat tatttcaaac tctgtatttt
     6781 gaataaaatc tattttgtct ataccaactt tttcttttat tattgataaa aaattttcgt
     6841 ttatttcata tccaattgaa tttctatata aatttttagc agcaagagtt gtagttccac
     6901 tgcccaaaaa agggtccaaa atagcatctt ctacaaaact gaacatttta attaatcttt
     6961 tgggtagttc ttctggaaac attgctaaat gttccgtttg cttttcaccg gtaaaattcc
     7021 aatgcccaga aaaatattca ttccattctt tttttgtaag tttggatttt tcttttaact
     7081 cttttgaatc ctttggggga tttccgggtt ttttaaatat taatataaat tcataatcaa
     7141 tctttataat accatttctt gggtaaggaa aactgcccat taaagtagca ccaccagtag
     7201 tattcatagt tgttggtttt tgccaaataa tatcccccat ataatcaaat cctattgttt
     7261 cacagaattt aattatttct gttcgtatag ggataatttt atatcttcca taatagacag
     7321 aacgagcaaa ttggtcgcct atatttatac atagacgaca accatcatat aataccctat
     7381 aacattcatt ccacaccaaa tttaaattgt ttatataatt ttcataagta tcattaaaac
     7441 caatctcatt tttaactccg taatctttta attgccaata tggtggtgat gtaataataa
     7501 gatgaactga tttatcggga atttcctcca tttttcgtga atcaccaatt attattttat
     7561 gttttgttac catatatttc tctttaagta taaaaccatt ctaaaagcgg agggggtggg
     7621 agtcgaaccc acgatcccag attgggatac acgctttcca agcgtgcgca ctcggcctct
     7681 atgcgacccc tccagaactt tttttatcag atgagaaaat tcttgcagga aactcataac
     7741 ctgcttttaa tcttttataa aaaagttttt tagaaagtcg atttaactga ctataagaaa
     7801 tttctcctgt ctctaatttt tttaattttt cctttgcttt tatttgtttc ttcttataaa
     7861 tttttcttac atctgtacta tattttccca tattggatat cctcctattt tggtatgtag
     7921 tttttaaaaa ttatattgga taaaccattt caggtcaaga aaaatgagaa ataaataata
     7981 gaagtgagat ttgtaatacc cctttcagtg agaaaaaata gaaatatttt tacaaaaatc
     8041 cgaattatag tttaatccaa aacgatatta tttctttaaa aacaagtggc aataaagcaa
     8101 atagacaatg aatttctgct attaatgaag aacggagata ttcattttta aaacttatgt
     8161 ttttaaagtt taaagacatt ggaaaccatc gcggaacttc tgacatatat ttacggtatt
     8221 gctcaccata caattccaat aaatgttgtt cttcatattt tacaacaagc gaatagacaa
     8281 ttacccataa tatgcctcct ttcaggtctt taggttattt cctaaaaggt tacatattta
     8341 aacacttttt acaatctttt tctccctatt agacccggtg cactatggtt gccgatagtt
     8401 agtacattat accctgcttg gtgacataaa ctgcctgtgt agaacaatcc ccctcaattc
     8461 ccgttgtgct tattgaacct ccaaaatccc cctcaatctc cgttgtgcta tctgaaatcc
     8521 ccctttatcc ctccgccaaa atgcgcgtcg gacaggcctt tgttaaaggg gaaaatgacg
     8581 caccaactct gaggcacaag ggaagcaact tttgtcggtt taataaagtg gggaaacttt
     8641 gtcttcaatt ttttcatttt gcacttctca catcccccct catcataatc ttctccccac
     8701 aggggcgaag aaaagacaat ccccctcacc ttctatcctc tccccacagg ggagaggctc
     8761 gcccgtaggg tcctcgcttt tggctacgga ttctaaacta ctcccgaggg gaagggtgag
     8821 gggggaatta atttttagta tatatttggt gtaaaaacag tatgaatatc acaatgccta
     8881 aaaaaatatg ggaatttagg attattaaaa aacatataag agatattaag aaattttagc
     8941 agtaccatgg aacgcaaaaa gaggtaaaaa tggaaaaagt aaaagaataa aaaattgata
     9001 tagtaataat gaaattagaa taaattgaaa attattagaa atttatgaaa ttaattccag
     9061 aagaaaattt agaagaatat gagaattcaa aagaaataaa aaaagcatat aattctgcaa
     9121 taaaaatcta tcccccaatt taatgaaaat agaaaaaaca aattacttta taaaagagta
     9181 taaaaaactc ccgtcaaaag ttaaatcact ttataaaaag caagaagaaa tatttatttc
     9241 aaattggtca aatatacttt tgtttacttc ttataattat tcccttattt taattctcta
     9301 aatgaatatt ttgggaaact tgacgattac aactaaatag tggtataatc ttaaaaaatt
     9361 atttatttat gggaaactat atttgtattt tttacaagac agtcagtgga aattcgccag
     9421 tagaagattt tattgaatct ttggatgaag atacacaaga taagtttatt tacaaaaagg
     9481 aattactgga acattttggt ccgcaattac gttgtccgca tacaagtgaa atttctgaag
     9541 gtatatttga gttgcgattt aaaggaaaag aggggcagat taggattttg tttttctttt
     9601 attatgagaa acaaattatt ctggtgcatg ggtttgtgaa gaaaacacag aaaacaccga
     9661 aaaaagaaat taatattgca aagaaaagga aaacagaata ttaccaaaga gtaaaagagg
     9721 aggagaaatt atgagaaaag tagatgatta tataaaagag aaaatgacaa aaaatccaga
     9781 ttttaaatct cgttatgaaa taataaaaca aaaagtagaa gttgctaaaa gaattattga
     9841 gtataggaat aaatataatt tatctcaatc tcaacttgcc aaaaagttag gtgtctcaca
     9901 acaatatatt tctaaaatag aagaaggaga gttttctaat atagagacgg tagaaaatat
     9961 tcttaaccaa ataggatatt gtctaaaatt agaagtagtt cctttatcta atcaagataa
    10021 aggttcttat caacaagttt taccataaaa ttgcgcacaa ataagaaact tgaaaaagtt
    10081 gagataccat aggtaacatt ccacagtttt ttgagaagtt caaggattca tataataaaa
    10141 gaaaggaagc agtgaagata gaagcagaag tggagttttg aaagaggtaa acaatttatt
    10201 acgccatatc taaccgcggg ggttgaacaa gaaggacact aaaatttgac ttttttaaga
    10261 ggacacttta gaaattttag tagttaaaaa tttatggata gttgcttctt gtttttacag
    10321 gaaaaaatag acattaccat gaaaagttct tatttcatat tgggaattaa actcaaaaaa
    10381 taacttttgc aggtaaatgt ttttagagat taattgaaaa tttggataat ttatagcaat
    10441 atactgaacc tttttaattt tgtaaagaag agtttaggtt tgcaattgtt gcctgaacat
    10501 tttttattct ctcttctata tctggatggt cgcttaaaa
//
LOCUS       DAOOVW010000054        10141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_4235_length_10141_cov_9.252132, whole genome shotgun sequence.
ACCESSION   DAOOVW010000054 DAOOVW010000000
VERSION     DAOOVW010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10141
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4235_length_10141_cov_9.252132"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..1556
                     /gene="purH"
                     /locus_tag="PLW95_07010"
     CDS             21..1556
                     /gene="purH"
                     /locus_tag="PLW95_07010"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOV22405.1"
                     /translation="MINIRRAILSVSDKRGIVELANFLKKFNVEILSTGGTAKILREN
                     GIEVKDVSEFTSFPEILEGRVKTLHPKIYGGILSIRDNEKHQKQMEENNLVPIDLIVC
                     NLYPFEETLKKGATDEEIIENIDIGGPTMIRAGAKNYKYVVVVIKPEMYKELMAEMGK
                     NNGKVSEDFSFRCAGYVFNFTSRYDYIISSYFKSKIEKKEFEDEIDIRLVKVSDLRYG
                     ENPHQVAAWYRFSDKEFERKQLQGKELSFNNLLDMESTYLLVNQFKENACVITKHTNP
                     CGVAIGENLVEAYEKALQTDPLSAFGGIIGFNRKVGIDVAKIIIKQFYEVIIAPDYEE
                     EALEIFKKKENMRIIKSPQIPTYKYDFRSLNDGFLIQTPDLIDMEKFEVVTEKKPTDE
                     EIEALKFSWVVCKFVKSNAIVIGTKGQTLGIGAGQMSRFDSARVAVMKMKDNFKEKVN
                     LVVMASDAFFPFPDSVEYVVKEGRVTAIIQPGGSLKDKEVIDVCNKLGVSMILTGTRH
                     FRH"
     gene            1604..2452
                     /locus_tag="PLW95_07015"
     CDS             1604..2452
                     /locus_tag="PLW95_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22406.1"
                     /translation="MKGSIFGWAVVGICVGIYLFFTGFRKLWYKRLIQNIPTSKIRSI
                     AMGLVEIQGIAIPDIILVSHYTKQQCVFYHIIMERLVIHKNSVNWVKEFEMKSNIPFF
                     LQDDTGTVIIDPVGAEIDIPLRYTHMEGNKRYKEYYIKIKEPIYVLGTAKSIESIEEH
                     IQKEVETRIKAIIESPEEKQKLDINKDMWIDENEWQLAKQRIMEEIKEKFRATQGKIS
                     SKNFPPDYLKNVIIGKGELDRHFFISNKSEKEIVGEYKYRVFLYIIGGAILTLLCLKI
                     VISFIF"
     gene            2467..3036
                     /locus_tag="PLW95_07020"
     CDS             2467..3036
                     /locus_tag="PLW95_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOV22407.1"
                     /translation="MGIIVAVFVAFVVIGLLLYFISVYNGLIRLSRDIEKNWANIDVL
                     LKQRYDEIGKLIKVCEGYMKYERETLEKIVGLRTEYLKTDSVEEKKKIDGEITGALKT
                     IFAVAENYPELKSNNNFIQLQNRISELENSIADRRELYNDSVNLYNIRIHQIPDKFVA
                     TMLNYKDKPLFEVSPEEKKDVEIKFDFPK"
     gene            3125..4090
                     /locus_tag="PLW95_07025"
     CDS             3125..4090
                     /locus_tag="PLW95_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001981311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HOV22408.1"
                     /translation="MKTEISGTTQYLWNDLPQLTEKEIFLSKLKGNGKRYKRYLGSPL
                     RYAGGKSWAVGYVIEKLPDNINRLISPFFGAGSIEIAVAKELGIDVLGFDIFDILINY
                     WQVQIKNPFKLYQKLLKLKPDKETYNKVKTILKEHWEGKRKLSSLKLATYYYFNHNLS
                     YGPGFLGWMSSVYANEKTYHNLIKRVKDFNVQNIKIECCSFEKVIPEFKNDFLYCDPP
                     YYLGKDSTLFKGLYPQRNFPIHHNNFNHKLLRDLLLNHRGGFILSYNDSPTIRNWYND
                     FVIVELPIHYTMGQGETRIGVNRKRDNRNHIKKTKELLIIKEQKR"
     gene            4087..4872
                     /locus_tag="PLW95_07030"
     CDS             4087..4872
                     /locus_tag="PLW95_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22409.1"
                     /translation="MTKKKAMTSAKASYVKRKGHADAREFAECLGIGKEFKNDPKAKK
                     DIIDSEGYSYSVKSGEKKWQIFLYGKTRFENDYTFKGMNGIGDLLLECIETFPSQRSD
                     YLQNKELYKKRLQEPMKKLCEKLKEKRILSAFIDKSMFNSGEVDFLVIKDGNIFHVFG
                     NRDVVEILTNLFEVENSKSRGEGQFDAQKVIFKVQDRTFGEIEMRNDSEVHYREIKFW
                     MDKKLIFNLLQSQIKQKGNFNDRIILYGSSIRKLLKLKQNKKS"
     gene            4915..6138
                     /locus_tag="PLW95_07035"
     CDS             4915..6138
                     /locus_tag="PLW95_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOV22410.1"
                     /translation="MEKYEVVVIGAGPSGIFSSIFAKQNKKEVSLIERNDTIGKKIFL
                     TGNGRCNLTSHNEIDEFISKYRNGKFLINAFSKFFNKDLIEFFEKNGLKLKVERGKRV
                     YPASDKAEDVVKTLKTIIDKRGVKLFLKNRVQNIEKAENKFIIKTDKNIIEAKKIVIA
                     TGGKSFPETGSEGDGYTLAKEFGHTIYGPFPALCGIEIKENFIKKWQGITLKNVKIKA
                     VLNKKVIGEEFGEVLFTHYGISGPAVLNLSGDVSENISKGEIKISINLKPALNYEKLE
                     KRLEREFISNPNKEIKNVFKNLLPNKMIDEFLYQCGIMTEKKANQITKEERKILVSNL
                     LSFNLSVKKTRDFSDSMVTRGGVVVKEINPKTMESKIVKGIYFAGEIIDIDGKTGGYN
                     LQASFSTGYVAGISV"
     gene            complement(6140..7015)
                     /locus_tag="PLW95_07040"
     CDS             complement(6140..7015)
                     /locus_tag="PLW95_07040"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOV22411.1"
                     /translation="MSPFIISLVLISAVMHAGWNLFARKDKDEITFFSQMLTVISVIG
                     FIPAFLTEFITHSLTLKAYLCVIGSGFFCGIYFFALANAYSFSDFTIVYPIARSLPVL
                     FVAIGDIIRGRDLSLIGWSGILLVVAGCFLSPLTSFKDISLKHYFNRTGFWLILTAIG
                     TTAYTLLDKIASEVVQKGSATAARYGYFFYFITCITYHILLHFSGIKKRKKGIEKWKL
                     PSLSAFLDFGSYWLILWVYQFTDITSYVIALRQFSILIGVILAFMIYKEKGVFVRIFG
                     TLLLTAGLIIIGVFT"
     gene            complement(7138..7222)
                     /locus_tag="PLW95_07045"
     tRNA            complement(7138..7222)
                     /locus_tag="PLW95_07045"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7187..7189),aa:Ser,seq:tga)
     gene            complement(7331..9379)
                     /locus_tag="PLW95_07050"
     CDS             complement(7331..9379)
                     /locus_tag="PLW95_07050"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HOV22412.1"
                     /translation="MEELIYKKVAKAANICKDKLIENVEEGELCFKQLLSMFPDDGII
                     YYRMGEGYKELFERTGNAVFKEKAIKSFQKAREILPFLAWQQKAENNLNQLIQDENIK
                     QLPEGNFVSPILYEVIKAVEIEIQRIKILKNRPHYYMFGKTFESERIINNIPYYEVGF
                     GNFHERIIIDAEFGKKNNIDLRIESGFIKGIISRHTNNEIVLLIPLTQISYEKISKAT
                     ENGIYYIADFKKSVFQLRNFLLKRRAVENEFILYLIRSATLPYNYGNYNHINLNKYKN
                     KYQFDQYKWQAIEKALTQNITFIWGPPGTGKTTVLAALAHILLEETDKNILFLTLSNS
                     TLDSLFIKVIEYVYKYSNKPYPEETFTRLGKIREKSTPKEIAKYYRDKISLNSRIVAA
                     NYQSLIFRNIKSAIFDYVIMDEVSMTPIPLLVAGSFFAKKNLILAGDPYQLPPPYPED
                     ADLPNDFYSMNVFEKVKIKKENDPRAVFLKTQYRMHQDISNLVSELFYDGKLECGNKK
                     IEKLSQNGSPFPKSVLFRNTEGVLEKIGSRIEIFNRKNDPYAFADVKLAKNIIKYCDI
                     KPEQIGIIVPYNAQVCNIYRYLKKENLEGIKVNTVHSFQGQEKDVIIYDITDDNIEPS
                     PLTTNWKFLNVALSRARRFLCVIGNQKYLMSSFFNAEEQERFRKIIEHGKWNITPYDL
                     "
     gene            complement(9423..9581)
                     /locus_tag="PLW95_07055"
     CDS             complement(9423..9581)
                     /locus_tag="PLW95_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22413.1"
                     /translation="MSKFVSFLYKLARTANDIEKITSGDPKKIARRAKNKILGRKVIS
                     KIYKWPKF"
     gene            10070..10141
                     /locus_tag="PLW95_07060"
     tRNA            10070..10141
                     /locus_tag="PLW95_07060"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10103..10105,aa:Lys,seq:ttt)
BASE COUNT         3809 a         1324 c         1678 g         3330 t
ORIGIN      
        1 ctaaaaaatg gaggaaaaaa atgattaata taagaagggc aattttaagt gtttctgata
       61 aaagagggat tgttgaactt gcaaattttt taaagaaatt taatgttgaa attttatcaa
      121 caggaggaac tgcaaaaatt ttgagagaaa acgggattga agtaaaagat gttagtgagt
      181 tcacttcttt tcctgaaata cttgagggaa gagttaagac acttcatcca aaaatttatg
      241 gaggaatttt atcaataaga gacaatgaaa aacaccaaaa acaaatggaa gaaaataatt
      301 tagtcccaat tgaccttatt gtttgtaatt tatatccatt tgaagaaaca cttaaaaaag
      361 gggcaacaga tgaagaaatt attgaaaaca ttgatattgg tggaccaaca atgataagag
      421 caggtgcgaa aaattataaa tatgttgttg ttgtgataaa accagaaatg tataaagaac
      481 ttatggctga aatgggaaaa aacaatggaa aagtaagtga ggatttttct tttagatgtg
      541 caggttatgt ttttaatttt acttcaagat atgattacat aatttcttca tatttcaaaa
      601 gcaaaattga aaaaaaggag tttgaagatg aaattgatat aagattggta aaagtaagtg
      661 atttaagata tggagaaaac ccacatcaag ttgctgcctg gtatagattt tctgataagg
      721 aatttgaaag aaaacaactt caaggtaagg aattatcttt caataatctt cttgatatgg
      781 aatcaacata ccttcttgta aatcaattta aagaaaatgc ctgtgtaatt acaaagcata
      841 caaacccatg tggagttgca ataggagaaa atttagtaga agcatacgaa aaagcattac
      901 agacagaccc tttaagtgct tttggtggaa taattggatt taataggaaa gttggaatag
      961 atgttgcaaa aataataatt aaacagtttt atgaagtaat tattgctcct gactatgagg
     1021 aagaagcact tgaaattttt aagaagaaag aaaatatgag aattataaaa tcacctcaaa
     1081 tacctaccta taaatatgat ttcagaagtt taaatgacgg atttttaatc cagacacctg
     1141 atttaattga catggagaaa tttgaagtcg ttactgaaaa aaaaccaact gatgaagaaa
     1201 tagaagcact taaattttca tgggtagttt gtaaatttgt aaaatcaaat gccatagtaa
     1261 ttggaacaaa agggcaaaca ttaggaattg gagcaggaca gatgtcaaga tttgattctg
     1321 caagagttgc agtaatgaaa atgaaagaca attttaaaga aaaagtaaat ttagttgtta
     1381 tggcatctga tgcttttttc ccatttcctg atagtgttga atatgtagta aaagaaggta
     1441 gagttactgc aataattcag ccaggtggtt cattaaaaga caaagaggta attgatgtgt
     1501 gtaataaatt aggtgtttca atgattttaa caggaacaag gcacttcaga cattaatagg
     1561 gacaccacaa ttatttcgtt tgtattggta taatatatcc aatatgaaag gaagtatttt
     1621 tggctgggca gtagtaggaa tttgcgttgg tatttatcta ttctttactg gatttcgtaa
     1681 attatggtat aagcgactaa ttcagaatat ccctacttct aaaattcgtt ctatcgcaat
     1741 gggactggtt gaaattcaag gaatagcaat acctgatatt attcttgtct ctcattatac
     1801 aaaacaacaa tgtgtttttt atcatatcat tatggaacga cttgtaatac ataaaaattc
     1861 ggttaattgg gtaaaagaat ttgaaatgaa aagtaatata ccttttttcc ttcaagacga
     1921 tacaggcact gtaataatag accctgttgg tgcagaaata gatatacctt taagatacac
     1981 tcacatggaa ggcaataaga gatataaaga atattacata aaaataaagg aacctattta
     2041 tgttcttgga acagcaaaaa gtatagaaag tattgaggaa cacatacaga aagaggttga
     2101 aacaagaata aaagcaataa ttgaaagccc ggaagaaaaa cagaaattag atatcaacaa
     2161 agacatgtgg attgatgaaa atgaatggca gttagcaaaa caaagaataa tggaagagat
     2221 aaaagagaaa tttagggcaa cacagggaaa aatatcctca aaaaatttcc ctcctgacta
     2281 tcttaaaaat gttattattg gcaaaggtga acttgacaga cattttttta tttcaaataa
     2341 aagcgaaaaa gaaatagtgg gtgagtataa atacagggta tttctttata tcattggtgg
     2401 tgctatactt actttgctct gtttaaaaat agtcatttct tttatatttt aaaaaggagg
     2461 gggaaaatgg gaattattgt agctgtattt gttgcttttg tggttatagg tctcttacta
     2521 tacttcataa gtgtctataa tggattgata cgcctgagta gagatataga aaaaaactgg
     2581 gcaaatatag atgttcttct taaacagcga tatgatgaga ttggaaaact gataaaggtc
     2641 tgtgagggat atatgaaata tgagcgagaa acattagaaa aaatcgtggg actaagaaca
     2701 gaatatttga aaactgattc agtagaagag aagaaaaaaa ttgatgggga aattacaggg
     2761 gcattaaaaa caatatttgc tgttgcagaa aattatccag aattaaaatc aaacaataac
     2821 tttatacaac tgcagaatag aatcagtgaa cttgaaaata gtattgcaga tagaagagaa
     2881 ttgtataatg atagtgttaa tctttataat atacgaattc atcagatacc tgacaagttt
     2941 gttgcaacaa tgttaaatta taaagataaa ccactttttg aagtaagtcc cgaagaaaaa
     3001 aaagatgtag agataaaatt tgattttcct aaatgactaa tgctctcacc ataaagaaat
     3061 aagataccca aaaattaaat tatttttgac agaaatatta aaaataagtt attttattta
     3121 taatatgaaa actgaaattt ctggcaccac tcaatattta tggaatgatt tacctcagtt
     3181 aactgaaaaa gagatatttt taagtaaatt aaaaggaaac gggaagagat acaaaagata
     3241 tttagggtct cctttaaggt atgcaggagg aaaaagttgg gcagttggat atgtaataga
     3301 aaaattacct gataatatca atcgcctaat atctccattt ttcggtgctg gttctattga
     3361 aattgcagtt gcaaaagaat taggaattga tgttcttgga tttgatatat ttgatattct
     3421 aataaattat tggcaggttc aaattaaaaa tccttttaag ttatatcaga aattattaaa
     3481 attaaaacca gataaagaaa catacaataa agttaaaacg atattaaaag aacactggga
     3541 agggaaaaga aaactttctt ctttgaaatt agcaacctac tattatttta atcataatct
     3601 ttcttatggt ccaggatttt tgggctggat gtcaagtgta tatgctaatg aaaaaacata
     3661 tcataatctt attaagagag ttaaggactt taatgtgcag aatataaaaa ttgagtgttg
     3721 ttcatttgaa aaagttattc ctgaatttaa aaatgacttt ttatattgtg acccccctta
     3781 ctatttagga aaagacagca ctctttttaa aggactttat cctcaaagaa attttccaat
     3841 ccatcataat aattttaacc ataaattact gagagacctt ttattaaatc atagaggcgg
     3901 atttatttta tcttataatg attcaccaac aataaggaat tggtataatg actttgtaat
     3961 tgttgaatta cccattcatt atacaatggg gcagggagaa acaagaatag gagttaatcg
     4021 taaaagagat aatagaaatc atatcaaaaa aacaaaagaa ttgttgataa ttaaagagca
     4081 aaaaagatga caaagaaaaa ggcaatgact tctgcgaaag caagttatgt aaaaagaaaa
     4141 ggacatgcag atgctcgtga atttgctgaa tgcttaggaa ttgggaaaga atttaaaaat
     4201 gacccaaaag caaaaaaaga cataattgac tctgaaggat attcatactc agtgaaaagt
     4261 ggcgaaaaga aatggcaaat ttttctttat ggaaaaacaa ggtttgaaaa tgattatact
     4321 tttaaaggta tgaatggaat aggagattta ttactggaat gtatagaaac ttttccttct
     4381 caaagaagtg attatttgca aaataaagaa ttatataaaa aaagattaca ggaaccaatg
     4441 aagaaattat gtgagaaatt aaaagaaaaa agaattcttt ctgcatttat agataaaagc
     4501 atgttcaata gtggagaagt tgatttttta gttataaaag atggaaacat ttttcatgtt
     4561 tttgggaata gagatgtcgt agaaatttta acaaaccttt ttgaggttga gaattctaaa
     4621 agtagaggtg aagggcaatt tgacgctcaa aaagtaattt ttaaagtcca agacagaact
     4681 tttggagaaa ttgaaatgag aaatgatagt gaggttcatt atcgcgaaat aaaattctgg
     4741 atggataaaa aattaatatt taatctcctt caatcccaaa taaaacaaaa gggcaatttt
     4801 aatgatagaa taattcttta tggtagttct ataagaaaat tattaaaact aaagcaaaac
     4861 aaaaaatcct aaaataaaca ttatcttcaa aggatttata aaaacaaatt aattatggaa
     4921 aaatatgaag tagttgttat tggggcaggt ccttcaggta ttttttcttc aatttttgcc
     4981 aaacaaaata aaaaagaagt atctttaatt gaaagaaatg ataccattgg aaagaaaatt
     5041 ttcttaacag gaaatggaag atgtaattta acaagtcata atgaaattga tgagtttata
     5101 agcaaatata gaaacgggaa atttcttata aatgcttttt caaaattttt caataaagac
     5161 ctgattgaat tttttgaaaa aaatggatta aaacttaaag ttgaaagagg gaaaagggtc
     5221 taccctgcat ctgataaagc agaagatgta gtaaaaacac taaaaacaat tattgataaa
     5281 agaggagtta aattgttttt gaaaaataga gtacaaaaca ttgaaaaagc agaaaataaa
     5341 tttattataa aaacagataa aaatataata gaggcaaaaa aaattgtaat agcaactggg
     5401 ggaaaaagtt ttccagaaac agggtcagaa ggtgatggat atacattggc aaaagaattt
     5461 gggcatacaa tttatggacc ctttcccgcc ttatgtggga ttgaaataaa agaaaatttc
     5521 ataaaaaaat ggcagggcat tacacttaaa aatgtcaaaa taaaggcagt tttgaataaa
     5581 aaagtaattg gagaagagtt tggagaagtt ttgtttactc attatggaat aagtggacct
     5641 gctgttttaa atttgagtgg agatgtttct gaaaatatat caaaaggaga aataaaaata
     5701 tcaataaatt taaagccggc cttaaattat gagaaacttg aaaagcgtct tgaaagagaa
     5761 ttcataagta atcccaataa agaaattaaa aatgttttta aaaatcttct tcccaataag
     5821 atgattgatg agtttttata tcaatgtgga attatgacag agaaaaaggc aaatcaaata
     5881 acaaaagaag aaaggaaaat acttgtttca aatttacttt catttaattt aagtgttaaa
     5941 aagacaaggg atttttcaga tagtatggta acaagaggag gagtagtagt aaaagaaata
     6001 aatccaaaaa caatggagtc aaaaattgtc aaaggtatat attttgcagg agaaataata
     6061 gatattgatg gaaaaacagg tggatataat ttacaggcgt ctttttcaac tggatatgta
     6121 gcaggaatct ctgtataaat tatgtaaaaa ctcctattat tataagtcct gctgtaagta
     6181 acaaagttcc aaaaattctt acaaagacac ctttttcttt ataaatcata aaagcaagaa
     6241 ttactccaat taaaatactg aattgtctta aagcaataac ataacttgta atatcagtga
     6301 attgatatac ccagagaata agccagtaag aaccaaaatc aagaaaagca gacagagatg
     6361 gaagtttcca cttttctatt cctttttttc ttttttttat acctgaaaaa tgtaaaagga
     6421 tatgataagt tatacaagtt ataaagtaga aaaaataccc atatcttgca gcagttgcag
     6481 accctttctg aacaacttca gatgcaattt tatcaagcaa agtataagca gtcgttccta
     6541 ttgctgttaa aattaaccaa aaacctgtcc gattaaaata atgttttaaa gagatatctt
     6601 taaatgaagt aagaggggat aaaaaacacc cggcaacaac aagaagtatc cctgaccatc
     6661 cgataagaga caaatctctt ccacggataa tatctccaat agcaacaaaa aggactggta
     6721 gagagcgagc gataggataa acaattgtaa aatcagaaaa actataagcg ttggcaagag
     6781 caaaaaaata tattccacag aaaaatccgg aaccaataac acaaaggtat gctttcaagg
     6841 ttagagaatg tgtaataaat tcagttaaaa aagcgggtat gaatcctatt acggatatta
     6901 cggttaacat ctgtgagaaa aaagttatct catctttatc ttttcttgca aataagttcc
     6961 atccagcatg cataacagca gaaattaaaa ctagcgatat aataaaaggg ctcatttaac
     7021 taaattcctg acaaaagaaa tattaaaatt taaaatattt gtgaaaatcg gattcataaa
     7081 cagaaaaaac atttttcccc tataacacct ttggacatgt ggtttctctc aaagaagcgg
     7141 agggtgaggg attcgaaccc ccatgtcctg tgaaggacgg cggatttcaa gtccgctgcc
     7201 ttacctgtta ggctaaccct ccaattactt ctattctcct ttttaaaatt ataccttctt
     7261 gattctattt ttcaaaattt atggtttttt cgtattttct ttataaattt gttgtttctt
     7321 attacaaatg ttataaatca taaggagtta tattccattt cccatgttct attatttttc
     7381 taaacctttc ttgttcctct gcattaaaaa acgaactcat tagatatttt tgatttccta
     7441 ttacacataa gaatcttctt gctcttgata atgctacatt aagaaatttc cagtttgttg
     7501 ttaaaggaga aggttcaata ttatcatctg taatatcata aattattaca tctttttctt
     7561 gtccttgaaa agaatgaacg gtatttactt ttataccttc taagttctct tttttaagat
     7621 acctgtatat gttgcagacc tgggcattat aaggaactat tattcctatt tgttcaggtt
     7681 taatatcaca gtacttaatg atattttttg caagttttac atcagcaaat gcataagggt
     7741 cattttttct attgaaaatt tcaattctac tacctatttt ttccaatacc ccctcagtat
     7801 ttctgaaaag aactgatttt ggaaatgggc taccattttg ggaaagtttt tctatttttt
     7861 tgtttccaca ttctaacttt ccatcataga atagttcact aacaagattg ctgatatcct
     7921 gatgcattct gtattgagtt ttcagaaata cagccctcgg gtcattttct ttttttattt
     7981 tgactttttc aaaaacattc attgaataaa aatcattagg caggtcagca tcttcaggat
     8041 aaggaggagg taattgatat gggtctccag caagaataag attctttttt gcaaaaaaac
     8101 tacctgccac caataaagga ataggggtca tggaaacttc gtccattatt acataatcaa
     8161 atatagcact tttaatattt ctgaaaatta aactttgata atttgctgct actattcttg
     8221 agtttaagga tatcttatct ctgtaatatt tagcaatttc cttaggagta gatttttcgc
     8281 ggattttacc caaccttgta aaagtttctt ctggatatgg tttattacta tatttataaa
     8341 catattctat aacctttata aataaagaat ccagagtact atttgaaaga gttaggaaaa
     8401 gaatattttt atctgtttct tcaagtaaaa tatgtgccaa agccgcaagt actgttgttt
     8461 taccagttcc agggggtccc cagataaatg ttatgttctg ggttaatgct ttctcaattg
     8521 cctgccattt atattggtca aattgatatt tatttttata tttattcaga ttaatatgat
     8581 tataatttcc ataattgtat ggtagagtgg cactcctaat taaatataga ataaattcgt
     8641 tttctactgc tcttctttta agaagaaaat tccttagttg gaaaacagat tttttaaaat
     8701 ctgctatata gtaaattcca ttttctgttg ctttacttat tttctcataa gaaatttgtg
     8761 ttagtgggat aagcaatact atttcattat tagtatgcct tgaaataatt ccttttatga
     8821 aaccactttc tattctcagg tcaatattat tctttttacc aaattcagca tcaataatta
     8881 tccgttcatg aaaattccca aaaccaactt catagtatgg tatattattt ataattcttt
     8941 cactttcaaa tgttttacca aacatataat aatgtggtct atttttgaga atttttattc
     9001 tttgtatttc aatttcaact gcttttataa cctcatataa aattggagaa acaaaatttc
     9061 cttctggtaa ttgttttata ttttcatcct gtattagttg attgagatta ttttcggctt
     9121 tttgttgcca ggcaagaaat gggagaattt ctcttgcctt ctggaatgat ttaattgctt
     9181 tttctttaaa tactgcattt cctgttctct caaataactc tttatatcct tctcccattc
     9241 tataatatat aatcccatca tcaggaaaca tacttaaaag ttgcttaaaa caaagttctc
     9301 cttcctctac attttcaata agtttatctt tacagatatt tgccgctttt gctacttttt
     9361 tataaataag ttcttccatt tataatggta tatttaaggt atctatttcc gtttgtaaat
     9421 cactaaaatt tgggccattt gtatattttg cttatgactt ttctacctaa aattttgttc
     9481 tttgctcttc gggcaatttt tttagggtct ccacttgtta tcttttctat atcattagca
     9541 gtccttgcca atttataaag aaaacttaca aattttgaca ttgttttcct cctgattttt
     9601 actttaacta taaacatttt acccttttaa aattaaaatg ataattcgtt atataaatta
     9661 ccgcaaaccc caaaatgata aaaaaggtag ggggggtgcg gaaaaggtag ttaaattcct
     9721 tgatacacct gctctttgac aacccacagt aagaatcact gacccgctta ataggggatt
     9781 atgaatagtg gcaaattttt catgtcactg tgaaaatttg cttaatttag aaaaaatata
     9841 acctcagaat taatcttata tatttataat taggtttttt atcttgttta atatggcttt
     9901 ttctatcagt ttattgtgtt tcaattcgct atcttattcc tatttttcga gtagattcct
     9961 tttttgtctt gtctaacata ccttttggag tagatttatt atatctcaat tcaatgtctt
    10021 gacatatctc ctttaaaaca ctaaaatata aaactcagag gtagttttgg ggccgctagc
    10081 tcatctggca gagcaccgcc cttttaaggc gggggtggca ggttcgagcc ctgcgcggcc
    10141 t
//
LOCUS       DAOOVW010000055         9922 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_4333_length_9922_cov_7.639201, whole genome shotgun sequence.
ACCESSION   DAOOVW010000055 DAOOVW010000000
VERSION     DAOOVW010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9922
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4333_length_9922_cov_7.639201"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="PLW95_07065"
     rRNA            <1..801
                     /locus_tag="PLW95_07065"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            996..2129
                     /gene="dnaJ"
                     /locus_tag="PLW95_07070"
     CDS             996..2129
                     /gene="dnaJ"
                     /locus_tag="PLW95_07070"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOV22414.1"
                     /translation="MSEKKDYYEILGVSRDASIDDIKNAYRNLVLKYHPDRNPGDKQA
                     EEKFKEITEAYEVLSDSEKRKIYDTYGHSGFGPGGFDWTQDFSRVQSDFSDIFGDIFG
                     DFFSDIFNTGRPSTRQRTVEKTRGSDLEAKIFITLNEVATGTEKYINISRYDPCPTCN
                     GTGSKSGVSKTTCPICHGTGKTTQSRGFFTITQTCSKCRGEGFIISNPCQNCRGTGRA
                     KNIHKILVKIPAGIENGTSLKLRGQGDIGQNNGERGDLYVTVYVEKNEIFERQGSDII
                     CEVPITIPQAVLGDEIEVPTLTGKVKVKIPPATQSGTLLRLKNLGVPRLAEYGKGDQI
                     LKIKVLIPKKLSREERNLYQQLKEIENHDNYPEIKEFKKKISE"
     gene            complement(2119..>2879)
                     /locus_tag="PLW95_07075"
     CDS             complement(2119..>2879)
                     /locus_tag="PLW95_07075"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOV22415.1"
                     /translation="MKEAIILGIIQGIFEWFPVSSSGHLIIFQKFFNFKVDITFDVFL
                     HLSSLVAIFIFFWKDIKEIGSKFFTFQKETYEFKIGIYIIISSIITGFIGFFLKDHKT
                     IFENFKLLSFAFLFTSILLLLTGIKRIEKNKKVDIKTSFITGIFQGVALIPGVSRSGA
                     TICSCLIAGVNREDTFRYSFLLGIPAILGAIIIEIPEIKKLYNPTFLLTGFTISLFIS
                     FVSLFLLRRVFLRGKFYLFGFYTLIVSILLLFIH"
     assembly_gap    2880..2979
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3042..3773)
                     /locus_tag="PLW95_07080"
     CDS             complement(3042..3773)
                     /locus_tag="PLW95_07080"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOV22416.1"
                     /translation="MNRKRNRRGFTLIELLVVLVIITILAGLLLPSVRQARKKALVDK
                     AKAEMTSLASAETMVRLDIGWYVRLCDLADGDLLDDYVTDSPHWGEKFGRTSSDGTFA
                     YYDVDKKEDDTGYESELTFEHPWDGPYQVFQTGTTYQRGKNGSVPKIDDNVEGWGDLE
                     DAVPDGTPLDPWGHTYLVAFDSYDSGTTDKKIEKVMIIYSAGPDGKLQTGAKATRPGD
                     RDDEDEDYEDEVEYPKSDDILYKFR"
     gene            complement(3870..4673)
                     /locus_tag="PLW95_07085"
     CDS             complement(3870..4673)
                     /locus_tag="PLW95_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOV22417.1"
                     /translation="MEKIKEIFYIGRERKFEEPAQQRNNKVQQTGFTLIELLVVIAII
                     AILASFLLPTLSSARERARRTTCMNNLKQFSLAYEMYAEDYYEKFPDQPDALYGTPDD
                     CIYTKYIKTTEIFWCPSSINRGNEAPPTIEGSTNWGGKNHSYYDWDNSYAFVFGLTTS
                     NNCSRPVPVISDKGIYSDAVTHGNHKDGMNVLYLEGNVIWVKVNELYDSDGDPIEDAF
                     SPASDGGPGKVACRSNKKDRNPSGDGPEDNYSIYLNDDDSDVDIEEWGQ"
     gene            complement(5008..6318)
                     /locus_tag="PLW95_07090"
     CDS             complement(5008..6318)
                     /locus_tag="PLW95_07090"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOV22418.1"
                     /translation="MPHFQYNAIDQSGNSLTGTIEAGHIQEAIGKLKSMGYYITNVAP
                     ARNVSAVSKSPDVKPNAPAKKTGKEKAKTKGKGQGMQIKLSFGPAKIKPKELMIITRQ
                     LATLIGSGLPLLRSLRVLKEQRKGGAAVVLGKVADDIESGALFSEALAKHPTSFPRIY
                     VAMIKAGEAGGALETVLARLAEFMEKEAKLKGKIKGAMAYPVVIVIVASGILTFIMMK
                     IVPTFVDIFKDFETDLPAPTILLINLSKLFTQKFYLIIIFVVSIFVLYKILTKIKFTK
                     YWIDMAKMKIPVFGPVVSKTIISRFARTFATLITSGVPILQSLLIVRDTVGNDVVAKA
                     INEVRNRVREGEGVSGPMMRTKVFPAMVTNMIAVGEETGAMDSMLEKVADAYDAEVDA
                     AVAAMTSMLEPMLIVCMGGVVGFIVISLFMPLIKLAMSMSGEGG"
     gene            complement(6330..8054)
                     /locus_tag="PLW95_07095"
     CDS             complement(6330..8054)
                     /locus_tag="PLW95_07095"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOV22419.1"
                     /translation="MPGKKLLGEMLIEQGLITEEQLRVALAEQRKSGNFLGRILVEMN
                     FVSEKDLKRVLSIQHGVEMVDLKNTVIDKKAIDSFPSALAKTYNVVPVKLEKDTLTLA
                     VGDTLSLNIQDDISFILGYKIKMVLADENDINDILDTYYGKEIETIDDLIKWLAQDIE
                     EMEELNAIASQGEYATITSLEEIASLPPVVKLFDLILLQTVRDNASDLHLEPFEDDFR
                     VRYRVDGVLYDLVHPPKGLSFALFCRFKIMAGMDIAERRLPQDGRIELSVMGRAVDLR
                     VNTVPTVFGECLAVRVLDRGKTIFELENLGLLEKDMETVESIIHKPYGIILATGPTGC
                     GKTTTLYAVLRKINTPDVKIITTEDPVEYMLEGAIQVPIRENIGLNFARCLRSILRQD
                     PDIIFVGEVRDFETAQMAIQSSLTGHLVLSTLHTNDAPSTIMRLMDMQAEPFLLASTI
                     EAVVAQRLVRVLCPRCKEEYKPTEIELLELDLTEEEVKKRNMKFYKAKGCPFCRGGYK
                     GRVAIFEILLPNDEFWKAVIGKKTLGEIREIAIKSCGMRTMLEDGLEKINRGITSVEE
                     VAKEIHGY"
     gene            complement(8056..9195)
                     /locus_tag="PLW95_07100"
     CDS             complement(8056..9195)
                     /locus_tag="PLW95_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013108690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOV22420.1"
                     /translation="MPKRIEELLELVVKENASDLHIAVGLPPMLRLHGGLVKADTDPL
                     TPEDTVNYMKAITSREHQEEVQKVGGTDFGFAFKDLARFRVSVYKDRGDIAMALRLIP
                     YTFLTFEQIGLEQRTMEYLLTRPRGLILVTGPTGSGKTTTLASMVNWINANLEKHIIT
                     IEDPVEYYHTHKRSIINQRELGVDVPTFAEALRRGLRQDPDVFLVGELRDLETIEAAV
                     TAAETGHVVFGTLHTTGASRTVDRMVNIFPVAQQEQIRVMLSVSILAIISQLLLPRVD
                     KKGRVAVFEIMVATPSIQNLIRERKTYRIVSEIQTGAKWGMVTLDACLMDLFKAGKIS
                     FDDVMIYAYDPESTKGQLIAEGIIDEEMAAKVIKQDGIQDVKVEK"
     gene            complement(9188..>9922)
                     /locus_tag="PLW95_07105"
     CDS             complement(9188..>9922)
                     /locus_tag="PLW95_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012991155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOV22421.1"
                     /translation="PTGSGKTTTLYASLQKLNTIDRKLMTVEDPVEYQIPGINQTPIN
                     ADIGLTFASVLRAFLRQSPDIILVGEIRDTETADIALRAALTGHLVFSTLHTNDAPSA
                     ITRLIDMGMAPFLIASSVQGVQAQRLVRILCPYCKEKIEPDEELKRKLKIKEGEEVNV
                     CRPKGCNECNFTGYHGRIAIFEIFIMNNELRELTLKRADISTLRQAARRSGMRTMYED
                     GIEKVKIGITTFEEVVSIVGEGGIDA"
BASE COUNT         3119 a         1955 c         1509 g         3239 t
ORIGIN      
        1 ctgaggcgcg aaagcgtggg gagcgaacgg gattagatac cccggtagtc cacgctgtaa
       61 acgatgggtg ctaggtactg gttcggtaaa acgaatcggt gtcgaagcta acgcgttaag
      121 cactccacct gggaactacg gccgcaaggc taaaacccaa aagaattgac gggggcccgc
      181 acaaccggtg gaacatgtgg tttaattcga tgatacacga agaaccttac ctgggcttga
      241 catctaccta gtaaaacccc gaaaggggaa tgatccgcct tatggcggaa gggtagacag
      301 gtgctgcatg gctgtcgtca gctcgtgccg tgaggtgttg ggttaagtcc cgtaacgagc
      361 gcaacccctg ctcgcagtta ctcgctcttc aagagcagca ctctgcgagg actgccgtgg
      421 ttaacacgga ggaaggtggg gatgacgtca agtcagcacg ccccttatgt ccagggctac
      481 acacgtgtta caatggctat tacaaaggga tgcaaaaccg cgaggcggag caaatcccaa
      541 aaaaatagcc ccagttcgga ttgcaggctg aaattcgcct gcatgaagct ggaatcggta
      601 gtaatcgcgg atcagccatg ccgcggtgaa tacgttctcg ggccttgtac acaccgcccg
      661 tcacgccaac tgagtcgagg atacccgaaa ttacatgcca tttgctttta gcaatatggg
      721 gtaatgaggg tatgctcggc aaggagggcg aagtcgtaac aaggcagccg taccggaagg
      781 tgcggctgga tcacctcctt tctaaggaga aaaaaaccgg ataccccttt ttatttgttt
      841 gtttcattcc ttctctcata ttttctcttc cttatgggaa aaaacataag aaaatatgag
      901 agaaggaaat ttttattaaa agcaaaaata ataaattatt tattgttata ttatttttcc
      961 atttctttta tttctgattt ttgaaaacaa aaacaatgag tgaaaagaaa gattattatg
     1021 aaattcttgg agtaagtcgt gatgcttcta ttgatgatat aaaaaatgcc tatagaaatt
     1081 tggttttaaa ataccatcct gatagaaatc ctggtgataa gcaagcagag gaaaaattca
     1141 aagaaattac agaagcatat gaagttctaa gtgactctga aaaaagaaaa atatatgata
     1201 catatggaca ttctggcttt ggtccgggtg gttttgattg gacacaggac ttttcaagag
     1261 ttcaaagtga tttttctgac atttttggag atatttttgg tgacttcttt tctgatattt
     1321 ttaatacagg acgcccttca acaaggcaga gaacagttga gaaaacaaga ggatctgatt
     1381 tagaagcaaa aatttttatc acattaaatg aagtagcaac aggaacagaa aaatatataa
     1441 acatttcaag atatgaccca tgtccaacat gtaacggaac tggaagcaag tcaggtgttt
     1501 caaaaacaac atgtccaatt tgtcatggaa caggcaaaac aacacaaagc agaggttttt
     1561 ttacaataac tcaaacatgt tcaaaatgca gaggtgaagg atttataatt tctaatccct
     1621 gtcaaaattg tagaggaacg gggagagcaa aaaatattca taaaatactt gttaaaatac
     1681 cagcaggaat tgaaaatggg acaagtttaa aattaagagg acagggcgat attggacaaa
     1741 ataatggaga aagaggggac ctttatgtga ctgtttatgt tgaaaaaaat gagatttttg
     1801 agagacaggg tagtgatata atatgtgagg tacctattac aataccacaa gcagttttag
     1861 gagatgaaat agaagttcca acattaactg gaaaagttaa agtaaaaatt ccaccagcaa
     1921 ctcagtcagg aactcttttg cgacttaaaa atttaggagt gccaagatta gctgaatatg
     1981 ggaaagggga ccaaatatta aaaataaaag ttctcatacc taaaaaatta tcaagagaag
     2041 aaagaaattt atatcaacaa ctaaaagaaa ttgaaaacca tgataattat ccagaaataa
     2101 aagaatttaa gaaaaaaatt agtgaataaa aagaagcaaa atagaaacaa ttagagtata
     2161 aaaaccaaaa agataaaatt tccctcttaa aaagaccctt cttaataaaa aaagagaaac
     2221 aaaacttata aataaagaaa ttgtaaagcc agttaataaa aaagttggat tataaagttt
     2281 ttttatttca ggaatttcaa taataattgc ccctaaaatt gcaggaatac ctaaaagaaa
     2341 agaatatcta aatgtatctt ccctatttac acctgctatt aaacaggaac aaatagttgc
     2401 acctgacctt gaaacaccag gtataagagc aaccccttga aaaataccag taataaatga
     2461 tgttttaata tctacttttt tatttttttc tattctttta attccagtta aaagaagcaa
     2521 aatagatgtg aataaaaaag caaacgataa gagtttaaaa ttttcaaata ttgttttgtg
     2581 gtcttttaaa aaaaagccaa taaatcctgt tataattgat gaaataataa tataaattcc
     2641 aattttaaat tcatatgttt ctttttgaaa agtaaaaaat ttactcccaa tttcctttat
     2701 atctttccag aaaaaaataa aaattgcaac aagagaggat agatgaagaa aaacatcaaa
     2761 ggtaatatct accttaaaat taaaaaattt ttgaaaaata ataagatgtc ctgaactact
     2821 aactggaaac cactcaaaaa tcccctgaat aatcccaagt ataattgcct ctttcatctn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gaaaccactc aaaaatcccc
     3001 tgaataatcc caagtataat tgcctctttc atctgggagg gttagcggaa tttatagagg
     3061 atgtcatctg actttggata ttctacttca tcttcatagt cctcatcttc atcatctcta
     3121 tctccaggac gagttgcttt cgctcctgtt tgtagtttac catctggacc tgcagagtaa
     3181 attatcatga ctttttcaat ttttttatct gtggttccag aatcatatga atcaaaagca
     3241 acaagataag tatgtcccca ggggtctaat ggtgtaccat caggaacagc atcttctaag
     3301 tcaccccagc cctcaacatt atcatctatt ttaggaactg aaccattttt acctctttga
     3361 tatgttgtcc ctgtctgaaa aacctgataa ggaccatccc atggatgctc aaaagtcaat
     3421 tcactttcat atccagtatc gtcctccttc ttatcaacat cataataagc aaaggtacca
     3481 tcagaagaag tcctaccaaa tttttctccc caatgaggag aatcagttac ataatcatcc
     3541 agaaggtcac catctgccaa atcacatagt cggacatacc agccaatatc taatctgacc
     3601 attgtttcag cacttgcaag ggaagtcatt tctgcttttg ccttatctac caatgccttt
     3661 ttccttgcct gtcttactga tggaagaaga agtcctgcca atattgttat tattaccaaa
     3721 acaacaagta attcaattag tgtaaaccct ctcctattcc tttttctatt catttttccc
     3781 cctattaaaa gatattatta cttcctcttt ttttaagaaa taatttttga gaaagtatag
     3841 ttcctcactt acatttttcg catattctct tattgtcccc attcttcaat atcaacatca
     3901 ctatcatcat catttaaata tattgaataa ttatcctctg gtccatcacc agaagggttt
     3961 ctatcttttt tattacttct acaggcaact ttgcccggac caccatcaga agcaggtgaa
     4021 aaagcatcct ctattgggtc cccatcagaa tcatataatt catttacttt tacccagata
     4081 acatttcctt ctaaatataa aacattcatc ccatctttat gatttccatg tgtaacagca
     4141 tctgaatata tacctttatc actaataaca ggaacaggtc ttgaacaatt atttgaagtt
     4201 gtaagtccaa aaacaaaagc ataagaattg tcccagtcat aatatgaatg gttttttcct
     4261 ccccaatttg tagaaccttc tatggttggt ggtgcttcat tacctctatt aatacttgat
     4321 ggacaccaga atatttctgt tgtttttata tattttgtat aaatacaatc atcaggggtt
     4381 ccatataaag catcaggttg gtctgggaac ttttcataat agtcctcagc atacatttca
     4441 taagcaagag aaaattgttt taaattattc atacaggttg ttcttcttgc tctttccctc
     4501 gcagaagata aagtaggtag aaggaaagaa gcaagtattg ctataattgc tattacaaca
     4561 agaagttcta ttaaggtaaa tcctgtttgt tgtactttat tatttctctg ttgggctggt
     4621 tcttcaaatt tcctctctct gccaatataa aatatctctt ttattttttc catttttcct
     4681 ccatcaattt ttccttggtt ctgccaaaat tggtcaatta aattccctcc acactttctc
     4741 acaaagacaa gtgaaatata aaaagtaaga tgtgagaagt ggaaatccac ccctgccctc
     4801 cttttttaaa ggaaggacta atttccccct ttagtaaagg gggattaagg gggatttata
     4861 aattaaaatc catcccctcg ggcatagccc tacgggcgag cccaccttcc ttttataaag
     4921 gaaggagtat tttccccctt gacaacgggg ggtgaagggg atttttcttt ttcatctatt
     4981 ttttgacagt acctcttcgt aacttcctca accaccttca cctgacatac tcattgctaa
     5041 ttttatcaat ggcatgaata gagaaataac tataaaacca acaactcctc ccatacatac
     5101 aataagcatt ggctcaagca tagaagtcat tgcagcaact gctgcatcaa cttctgcatc
     5161 ataagcatct gcaacttttt caagcataga atccatagca ccagtttctt caccaactgc
     5221 aatcatattt gttaccatag caggaaaaac ttttgttctc atcataggtc ctgatacacc
     5281 ttcaccttct cttactctgt tccttacttc atttattgcc tttgcaacaa catcattacc
     5341 aacagtatct cttactataa gtaatgactg taaaattggt actccacttg ttattagagt
     5401 tgcaaaagtt cttgcaaatc ttgaaataat tgtttttgaa acaactggac caaaaacagg
     5461 aattttcatt tttgccatgt ctatccagta ttttgtgaac tttatttttg tcaaaatttt
     5521 ataaagcaca aatatagaaa caacaaatat aataataaga taaaatttct gagtaaaaag
     5581 ttttgaaagg tttataagta agattgttgg agccggtagg tctgtttcaa aatccttaaa
     5641 aatatctaca aatgtaggaa ctattttcat catgataaat gttaatatac cagaagcaac
     5701 aataactatt acaacaggat atgccattgc cccttttatc ttccctttca attttgcctc
     5761 tttttccata aattcagcaa gtcgggctaa tactgtttca agcgctccac ctgcttctcc
     5821 tgcctttatc attgcaacat aaattcttgg aaaacttgta gggtgttttg caagtgcctc
     5881 tgaaaataaa gcaccacttt caatatcatc tgcaactttt cccagaacaa cagcagctcc
     5941 tccttttctt tgttccttta agaccctcaa tgaccttaaa agagggagcc ctgaaccaat
     6001 aagagttgct aattgtcttg taataatcat tagttctttt ggtttaattt ttgccggacc
     6061 aaaagatagt tttatctgca ttccctgccc tttacctttt gtttttgctt tttctttccc
     6121 tgtttttttg gcaggtgcat ttggtttcac atcaggagat ttagatacag cagatacatt
     6181 tcttgcagga gcaacatttg taatataata acccatactc tttaatttac ctatggcttc
     6241 ctgtatatgt cctgcttcaa ttgttcctgt taaactattt cctgattggt caattgcatt
     6301 atattggaaa tgtggcattt tccccctcct taataaccat gaatttcttt tgctacctct
     6361 tctactgaag ttatacctct atttatcttt tcaagtccat cttcaagcat cgttctcata
     6421 ccacaacttt ttatagcaat ttctcttatc tctccaagtg tctttttccc tatgactgcc
     6481 ttccagaact catcatttgg aagtaaaatt tcaaaaattg caactcttcc tttataacca
     6541 cctctacaaa atggacaccc ctttgcttta taaaacttca tattcctttt tttaacttct
     6601 tcttctgtta aatctaattc aagtaattct atttctgttg gtttatattc ttctttacat
     6661 cttggacaca gaactcttac aagtcgctga gcaacaactg cttcaatcgt agatgcaagt
     6721 aaaaatggtt cagcttgcat atccataagt cgcataattg tacttggagc atcatttgta
     6781 tgtaatgtac ttaaaactaa atgtcctgtg agtgatgatt gtatagccat ctgtgctgtc
     6841 tcaaaatccc ttacctctcc aacaaaaata atatcagggt cctgccttaa aatacttctt
     6901 aaacatcttg caaaattaag tcctatattt tctcttattg ggacctgaat tgctccttca
     6961 agcatatatt ctacagggtc ctctgtggta ataattttta catcaggagt atttattttc
     7021 ctcaaaacag cataaagagt tgttgttttt ccacaaccag ttggacctgt tgcaagaatt
     7081 ataccatatg gtttgtgaat aatgctttca acagtttcca tatctttttc aagtaatccc
     7141 aaattttcaa gttcaaatat cgtttttccc ctatcaagaa ctctcactgc taaacattct
     7201 ccaaaaacag ttggaactgt atttactcta aggtcaactg ctctccccat aactgaaagt
     7261 tcaattctac catcctgtgg aagacgcctt tctgctatat ccataccagc cataatttta
     7321 aatctacaga aaagtgcaaa tgaaagtcct tttggaggat gaacaaggtc atacaaaaca
     7381 ccatcaactc tatatcttac tctaaaatca tcttcaaatg gctctaaatg aaggtctgat
     7441 gcattatctc ttactgtttg taaaagtata aggtcaaaaa gttttacaac tggcggaaga
     7501 gaagcaattt cttccaatga tgttattgtt gcatattctc cctgagaagc aattgcattt
     7561 agttcttcca tttcttcaat atcctgagca agccatttta taaggtcatc aattgtctct
     7621 atttcttttc cataatatgt atcaagtata tcatttatat cattttcatc tgcaagaacc
     7681 attttaattt tatatcccaa aatgaatgat atatcatcct gaatatttaa acttaaagta
     7741 tctccaactg ctaatgttaa tgtatctttt tcaagtttta ccggtacaac attatatgtt
     7801 tttgcaagag cagaagggaa agaatcaatt gcttttttat caattactgt attcttcaaa
     7861 tcaaccattt caaccccatg ttgaatactt aaaactcttt tcaagtcctt ttcagaaaca
     7921 aaattcattt caactaatat cctgcctaaa aaattcccac tttttctttg ttctgctaaa
     7981 gcaacccgca attgctcttc tgttattaat ccctgctcta ttaacatttc ccctaaaagt
     8041 tttttacctg gcattttatt tctccacttt tacatcctgt ataccatctt gctttatcac
     8101 ctttgctgcc atttcttcat ctataattcc ttctgcaata agttgacctt ttgttgattc
     8161 agggtcataa gcataaatca taacatcatc aaaactaatt tttcctgctt taaaaaggtc
     8221 cattaaacag gcatctaatg ttaccatccc ccattttgcc cctgtctgaa tttcagatac
     8281 aattctatat gttttccttt ctctaattaa attttgaatt gaaggagttg caaccattat
     8341 ttcaaaaaca gcaactctgc cttttttatc aactcttgga agaagtaatt gtgaaataat
     8401 tgcaagaatt gaaacagaaa gcataactct tatctgttcc tgctgtgcaa cagggaaaat
     8461 atttaccatt ctatcaactg ttcttgaagc acctgttgta tgaagtgttc caaacacaac
     8521 atgccctgtc tctgctgctg taactgctgc ttcaattgtt tcaaggtctc ttaattcacc
     8581 aacaaggaat acatcagggt cctgtcttaa acctctcctt aatgcttctg caaaagttgg
     8641 gacatctact cctaattctc tttgattgat tattgacctt ttatgagtat gataatactc
     8701 aacagggtct tcaattgtaa taatatgttt ttcaagattt gcatttatcc agttaaccat
     8761 agacgctaat gtcgttgttt ttccacttcc tgttggacct gttacaagaa taagccctct
     8821 tggtcttgtt agaagatatt ccattgttct ttgttcaagc cctatttgct caaatgtcaa
     8881 aaatgtatat ggaattaatc ttaaagccat tgcaatatcc cctctatctt tgtaaacact
     8941 aaccctaaat cttgcaaggt ctttaaaagc aaacccaaaa tcagtcccac ctactttttg
     9001 tacttcttct tgatgctctc ttgaagttat tgctttcata taattaaccg tatcttcagg
     9061 agttaatggg tcagtgtcgg cttttacaag tcctccatga agtcgcaaca ttggtgggag
     9121 tccaacagca atatgcaggt cagaagcatt ctctttaaca acaagttcaa gtaattcctc
     9181 aattctttta ggcatctatt cctccttctc caacaataga aacaacttct tcaaaagttg
     9241 ttattcctat tttaactttt tcaattccat cttcatacat agttctcatt ccagaccttc
     9301 ttgctgcttg ccttaaagta gatatatctg ctctttttaa tgttagttct cttaattcat
     9361 tattcataat aaatatttca aaaattgcta ttcttccatg ataaccagta aaattacatt
     9421 cattacatcc ttttggacga cagacattga cttcttcgcc ctctttaatt tttaattttc
     9481 tctttaattc ctcatctggc tcaatttttt ctttacaata tggacaaagt attctaacaa
     9541 gtcgctgtgc ctgtacaccc tgaacggatg aggcaataag aaaaggtgcc attcccatat
     9601 caatcaatct tgttattgca cttggagcat catttgtatg aagtgttgaa aaaacaaggt
     9661 gtcctgtaag ggcggctctt aaagcgatat ccgctgtttc tgtatctcta atttctccaa
     9721 ctaatataat atccggggac tgccttaaaa aagcacgcaa aacacttgca aaagtcagac
     9781 caatatcagc atttattggt gtttgattaa tccctggaat ttggtattca acagggtctt
     9841 caacagtcat taatttccta tctattgtat ttaatttctg taaggaagca taaagagttg
     9901 ttgtttttcc acttcctgtt gg
//
LOCUS       DAOOVW010000056         9885 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_4349_length_9885_cov_8.306714, whole genome shotgun sequence.
ACCESSION   DAOOVW010000056 DAOOVW010000000
VERSION     DAOOVW010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9885
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4349_length_9885_cov_8.306714"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1279
                     /locus_tag="PLW95_07110"
     CDS             <1..1279
                     /locus_tag="PLW95_07110"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOV22422.1"
                     /translation="SEGASGKEFTSKNKVKVIYKKIPDFHSVAVGVFVKVGSRTEDEE
                     ISGISHFVEHILFKGTKKRTSNQIKEEIEGVGGMFNAFTSEEATCYWIKILDEYLEKT
                     FDVLSDMIENPALKKEDIEKERAVILEEIKMYKDIPAKYVHEIFDSMLFEGHHLGRPI
                     AGTEETVKKITKEDMEKYMNELYGSSNIVISVAGNFSENKIRDLSKKYFKLRNGRKIT
                     FPKWQGNESGPKVKVLKKDTEQTHIVIGGLGYSREDNRKYPLCVLNTILGGNMSSRLF
                     NKIREEMGLAYEIGSFIRNYTDTGAFIISAGVKHSNSLKATEAIIGELVKIRNEGVND
                     DEIERSKKYLISHTLMGLEGNLEYMMFLGEQRLLREKITSVEEIRKKIEEVTKEDVKE
                     VAEQLFTQKNFYLAMISPYGEGKKFLKITDELK"
     gene            1303..1944
                     /locus_tag="PLW95_07115"
     CDS             1303..1944
                     /locus_tag="PLW95_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HOV22423.1"
                     /translation="MSNEILNGIKESVIVGDAKKTEELVNKAVKENIEVKKILDEGLI
                     AGMMVVGEKFKNNEYYVPEVLIAARAMHKGMDIIEPLIVQAGIKPVAKLAIGTVKGDL
                     HDIGKNLVVMMWKGAGFEVEDLGIDVPPEKFVEAANRGTQIIGMSALLTTTMIAMKEV
                     IEKLKETGIRDKVKIIIGGAPVTQEYADEIGADAYAPDAATGVDKVKELLNIK"
     gene            1995..2606
                     /gene="plsY"
                     /locus_tag="PLW95_07120"
     CDS             1995..2606
                     /gene="plsY"
                     /locus_tag="PLW95_07120"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOV22424.1"
                     /translation="MDILKIFFLFIFSFILGSIPFGYVLCKIIKKIDIRKYGSGNIGA
                     TNVYRVCGGFLGSSVLFLDIIKGYIPVFISRNFFEFSSIISILIGLAAILGHTFSPFL
                     KGKGGKGISTSFGVIIALFPLSAFCSFIIFVLVLFIFHIVSVASITASFFLPISIYIS
                     SKDIPLTIFGIIIFIFIIYTHRENIKRLKKGKEKRIILPWEKK"
     gene            2591..3577
                     /locus_tag="PLW95_07125"
     CDS             2591..3577
                     /locus_tag="PLW95_07125"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOV22425.1"
                     /translation="MGEKIGILGSGAWGICLSLLLSKKNNEITLWEPIKDNYEILIKE
                     RESTIYFPGVKIPENVVITNSIEETIKNSNNLIITVRSTFFKETIEIIEKHYSDQKIL
                     VGTKGMEVKTKKFFSEIIEERRIVKNYGIISGPTIAREIVYGFPSAAVIASNCKDISL
                     YFQKLLSSDIFRVYITKDIKGVQVGGSFKNVIAIGAGIIDGLQLGMNTKASYLTRGLN
                     EMIKVGRKLGGKEKTLRGLSGIGDLITTSFSKYSRNRSFGEGIIREGKENYLEKTKMV
                     IEGIYATDVYYTLSHRLKVELPITSSIYRIIFSNSSPTEEINYLMKRKLKRE"
     gene            3574..3897
                     /locus_tag="PLW95_07130"
     CDS             3574..3897
                     /locus_tag="PLW95_07130"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOV22426.1"
                     /translation="MNEEKEKFFYIGDVSKITGIPEHILRYWEGEFRILKPIRDQRGH
                     RLYSEKDIEKIKNIKLLIYKEGYKIKGAKKKIKTKEDKKSKKGIVDFLNYLIKEIEGI
                     KKCLK"
     gene            3885..4493
                     /gene="purN"
                     /locus_tag="PLW95_07135"
     CDS             3885..4493
                     /gene="purN"
                     /locus_tag="PLW95_07135"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOV22427.1"
                     /translation="MFKIGVLASGKGTNLKAIIDYIKGEKASFEIGIVLSDNLDANAL
                     TIAKNEGIKAEYISPGRYKTYLEPEIEKKYVNILKENKVELVCLAGFMRVIKKTFLEN
                     FSNKIINIHPSLLPSFPGIEAWKQALEYGVKFTGCTVHFVNNRIDAGPIIMQAVVPIL
                     SDDTPGSLHKRIQEKEHIIYPLSINLISEGRVSISGRKVLIK"
     gene            4550..5029
                     /locus_tag="PLW95_07140"
     CDS             4550..5029
                     /locus_tag="PLW95_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012963025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOV22428.1"
                     /translation="MHTNQDIKIRKATIKDTKHIKKIVDKFADEEKMLPLSYNQIYER
                     IRDFWVIESGKQIIGCGSLKPTWKDLAEIRSLAVKENFQKMGYGKLLIENILKEGEQL
                     GFKKIFVLTYKPGYFEKFGFKKISKSKLPHKIWFDCINCPKFPKCDETALIKELIKK"
     gene            5026..6129
                     /locus_tag="PLW95_07145"
     CDS             5026..6129
                     /locus_tag="PLW95_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22429.1"
                     /translation="MKKLILIIFLLFFPSILESKVFFGENIKIESIKEDIFALGEKIE
                     AKGNLENEFIGIGRNILSDISLNGDYIGIGLNQKLKCESKSDVYMLGNNVETTGNIKG
                     SIAIFGSIISLKGNIEKNLRLFGEKIFLDTNVDGNTVIWGQNIYLKGNFGNLVIHSDE
                     INFNEMSVINGNLVYYSKKEMDFKNVKIKGEKIWKKPMSETIKEKMPILKLKSFYNFL
                     SLLFPFFFMLWFTPNVLSQTAIYSGKKFLYCFGLGIILILITLIIVPIIFVTIIGIPF
                     GLIVSSIFISCVYVSRVFPLIFVGRKILFKLPDKRITWFFSTFIGVLFFTLISIIPNL
                     KLLLNLITIPVGFGAIVLGRVDQFKKLKKANFL"
     gene            6143..7081
                     /locus_tag="PLW95_07150"
     CDS             6143..7081
                     /locus_tag="PLW95_07150"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOV22430.1"
                     /translation="MKIKIISNLIKEVNEKNLSNNLFYLAKNPLPFRKLNYTIPGHSK
                     NTLYETDDFIENELAKYGYKIEKEEGKVQAYGRDITKPKSSQYAPPSPESPWYTAHNL
                     YAKKKGKTYPDEIILIVSHKDSQSWVDSPGAYDNAVGVVSNMEIARILSNYESVRSLY
                     FLFCNEEHTPWTSITAAKNAKTRGDNLIMIINIDSIGGKSKEEAETGAKTNVTLYTTD
                     EGEKIADLMIELNKIYNIGLIQRKYKRTSPGDDDGSFIKEGYKVAVANIGSYPYADPN
                     YHDEKDIPEFVDIQNVKMSTQAILSSILTIDKEGIK"
     gene            7078..8283
                     /locus_tag="PLW95_07155"
     CDS             7078..8283
                     /locus_tag="PLW95_07155"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOV22431.1"
                     /translation="MKIVVAYSGGLDTSIIIKYLIEKYNAEVIAFTANIGQKIKEKEL
                     EKKALKTGAKKFYFKDLREKFVKNYIIPSLKGSALYQDKYPMATSLSRPLIAEEMVEI
                     AKKEKVDAVAHGCTGKGNDQVRFELTFKYLSPELKIIAPLREWDFKSREEEIEYAKKN
                     KIPIPVSKEKPYSIDENLWGISIECGKLEDPWEQPPDDAYQITISPEKAPDKPTYIEI
                     EFEKGVPVGLNGKKMDILKIIEFLNKVGGENGVGRIDMVEDRVVGIKSREIYEAPAAV
                     ILHTSHSELEKLIFTKELWEFKKLVSIKFSQLIYYGNWFSDFRECLTAFVEETQKYVT
                     GKVKVKLYKGNCEVVGRKSPYSLYIEELATYTEKDIFDHKASNGFIEIFGLSALLEGR
                     RKLKTKKEK"
     gene            complement(8813..9616)
                     /gene="istA"
                     /locus_tag="PLW95_07160"
     CDS             complement(8813..9616)
                     /gene="istA"
                     /locus_tag="PLW95_07160"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HOV22432.1"
                     /translation="MAGRRIKVIDIREMLRCLRMKESNRRIAKMLGINRRTVSKYRQW
                     ANWHNLLEGELPSLEIIEKFLEESGMIGIGERKGSKVVPYHDRVEELISKNCSIQVIY
                     ERLRDNYGFKGSYDSVRRYIKNIREKESDKYFRIETAPGEEAQVDFGYSGLMYDFVEK
                     RLRKAWCFGMVLSYSRHIFVKFVFDQKISTWLRLQQDGFEYFEGVVKKVVLDNLKAAV
                     VKAAVYDTVLQRSYLEFAEHYGFLVSPCRIGTPRHKGKVKNADQRSGLL"
     gene            complement(9677..>9885)
                     /locus_tag="PLW95_07165"
     CDS             complement(9677..>9885)
                     /locus_tag="PLW95_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22433.1"
                     /translation="EKALKEVWYILDCPCGRRLGDYIGEIVPILEKKGEIKITEEGRC
                     HQAGYNVLTIGTHSAPGRIGRKRL"
BASE COUNT         3793 a         1296 c         1680 g         3116 t
ORIGIN      
        1 gtcagaagga gcatctggaa aagaatttac atctaaaaat aaagttaaag taatatacaa
       61 aaaaattcct gattttcatt ctgttgctgt cggtgtgttt gtaaaagttg gaagtagaac
      121 agaagatgaa gaaatttcag gaatttcaca ctttgttgaa catatattat tcaaaggaac
      181 aaagaaaaga acgagtaatc aaataaaaga agaaattgaa ggagttggcg gtatgtttaa
      241 tgccttcaca tctgaagaag caacatgtta ttggataaaa attcttgatg aatatttaga
      301 aaaaacattt gatgttttat cagatatgat tgaaaatcct gctttaaaga aagaagatat
      361 tgaaaaagaa agggctgtta ttcttgaaga aataaaaatg tataaggata tacctgcaaa
      421 atatgttcat gaaatttttg attccatgct ttttgaaggg catcatttgg gaagaccaat
      481 tgcagggacg gaagaaacag ttaagaaaat tacaaaagaa gatatggaaa aatatatgaa
      541 tgaactttat ggttcttcaa atattgtaat aagtgttgct ggaaatttct ctgaaaataa
      601 aatcagagat ttaagcaaaa aatattttaa attaagaaat ggaaggaaaa ttacctttcc
      661 aaaatggcag ggaaatgaaa gtggaccaaa agtaaaagtt ctaaaaaaag atactgaaca
      721 aactcatatt gtaattggtg gtcttggata ttcaagagaa gataatagaa aatatccatt
      781 atgtgtttta aatacaattc ttggtggaaa tatgagcagt cgtcttttta ataaaataag
      841 agaggaaatg ggacttgctt atgaaatagg aagttttata aggaattata ctgacacagg
      901 tgcttttata atttctgctg gtgtaaaaca ttcaaattca cttaaagcaa cagaagcaat
      961 tattggagaa cttgtaaaaa taagaaatga aggggtaaat gacgatgaaa ttgaaagaag
     1021 taaaaaatat ttaatttccc atactcttat gggacttgaa gggaatcttg aatacatgat
     1081 gtttcttgga gaacaacgac ttttaagaga gaaaattaca tctgtggaag aaataagaaa
     1141 aaaaatagaa gaagttacaa aagaggatgt taaagaagtt gcagaacaac tttttactca
     1201 gaaaaatttt tatttagcaa tgatttctcc ttatggagag ggaaaaaaat ttctaaaaat
     1261 aactgatgaa ctaaaataaa aactttaaaa aggaggattt aaatgtcaaa tgaaatttta
     1321 aatggaatta aagaaagtgt tatagtggga gatgcaaaaa aaacagaaga attagtaaat
     1381 aaagcggtaa aagagaatat tgaagttaaa aaaattttag atgaaggact tattgcaggg
     1441 atgatggttg ttggagagaa gtttaaaaat aatgaatatt atgttccaga agttctaatt
     1501 gctgccagag caatgcataa aggaatggat attatagaac cattaattgt tcaggcagga
     1561 ataaaaccag ttgcaaaatt agcaatagga acagtaaaag gagacctgca tgatataggt
     1621 aaaaatcttg tagttatgat gtggaaaggt gctggatttg aagtagaaga ccttggaatt
     1681 gatgttccac cagaaaaatt tgttgaagca gccaatagag gcactcagat tattggaatg
     1741 agtgcccttt tgacaacaac aatgatagca atgaaagaag tgattgaaaa gttaaaagaa
     1801 acaggaataa gagataaagt taaaattatt attgggggcg ctcctgttac acaggaatat
     1861 gcggatgaaa ttggtgcaga tgcttatgca ccagatgctg ccacaggtgt agataaagtg
     1921 aaagaattgc taaatattaa ataagaaggg atagggtata tattggtaaa ttttaaatat
     1981 atttcaaaaa tcttatggat attttaaaaa tattttttct ttttatcttt tcgttcattc
     2041 ttggaagtat accttttggt tatgttttat gtaaaattat taaaaaaatt gacataagaa
     2101 aatatggaag tggaaatatt ggtgctacta atgtttacag ggtatgtgga ggatttttag
     2161 ggtcatctgt tttatttctt gatataataa agggctatat ccctgttttc atttcaagaa
     2221 atttttttga attttcttct ataatttcaa tattgatagg acttgctgct attttagggc
     2281 atactttttc tcctttttta aaaggtaaag gcggaaaagg aatttcaaca agttttggtg
     2341 ttataattgc tcttttccca ttatctgctt tttgttcttt tattattttt gttcttgttt
     2401 tgtttatttt tcatattgtt tctgttgcat caattactgc aagttttttt ctcccaattt
     2461 ctatttatat ttcctccaag gatattcctt taactatttt tggaataata atttttattt
     2521 ttattatcta tactcataga gaaaacatta aaagattaaa aaagggaaag gagaaaagga
     2581 taattttgcc atgggagaaa aaataggtat tttaggaagt ggtgcttggg gaatttgttt
     2641 atcactacta ctatcaaaaa aaaataatga aattactctc tgggaaccaa taaaagataa
     2701 ttatgaaatt cttataaaag agagggaaag tacaatttat ttccctggtg taaaaattcc
     2761 agaaaatgta gttataacaa actctattga agaaacaata aaaaactcta ataatttaat
     2821 tattacagta agatctactt tttttaaaga aacaattgaa ataattgaaa aacattattc
     2881 agaccaaaaa attcttgttg gaacaaaagg aatggaagta aagacaaaaa aattcttttc
     2941 tgaaataata gaagagagaa gaattgttaa aaactatgga attatttcag gaccaacaat
     3001 tgcccgtgaa attgtatatg gttttccatc agcagcagta attgcttcaa actgtaaaga
     3061 tatatcttta tattttcaaa aacttttaag cagtgatatt ttcagagtat atattacaaa
     3121 agatataaag ggagttcagg taggtggttc ttttaaaaat gttattgcaa ttggagcagg
     3181 tattattgat ggactccaat tgggtatgaa cacaaaggca tcatatttaa caagagggtt
     3241 gaatgaaatg ataaaagtgg gcaggaaact tggaggaaaa gaaaaaacat taagagggct
     3301 ttcaggtatt ggagacctga taacaacttc ttttagtaaa tatagcagaa atagaagttt
     3361 tggagaagga attataagag agggcaaaga gaattatctg gaaaaaacaa aaatggtaat
     3421 tgaaggcata tatgcaacag atgtctacta taccctttct catagattga aagttgaact
     3481 tcctataaca tcatcaattt acagaataat tttttcaaat agcagcccaa cggaagaaat
     3541 aaattattta atgaaaagga aacttaaaag agaatgaatg aagaaaaaga aaaatttttc
     3601 tatattggag atgtaagtaa aataacagga atacctgaac atattcttag atactgggag
     3661 ggagaattta gaattttaaa gccaataaga gatcagagag gacatcgtct ttatagtgaa
     3721 aaagacattg aaaaaattaa aaatataaag ttgcttattt ataaagaggg ttataaaata
     3781 aaaggtgcaa agaaaaaaat aaaaacgaaa gaggataaaa aaagtaaaaa aggaattgtt
     3841 gattttttaa attacttaat aaaagaaata gaagggataa aaaaatgttt aaaataggag
     3901 ttcttgcttc tggaaaaggg acaaatttaa aggcaatcat tgattacata aaaggtgaga
     3961 aggcatcttt tgaaataggg attgttttaa gtgataattt agatgcaaat gcattgacaa
     4021 tagcaaaaaa tgaaggaata aaagcagaat atatttcacc aggaagatac aaaacatatc
     4081 ttgaaccaga aattgagaaa aaatatgtaa atattctaaa agaaaacaaa gtagaacttg
     4141 tatgcctggc tggttttatg agagtgataa aaaaaacatt tttagaaaat ttttcaaata
     4201 aaattataaa tatccaccct tcccttcttc catcttttcc tggtattgaa gcatggaaac
     4261 aggcacttga atatggtgta aaatttactg gttgtactgt ccattttgtt aataatagaa
     4321 ttgatgcagg ccctattata atgcaagcag ttgttcccat tttatcagat gatacacctg
     4381 gatcattaca taaaagaatt caggaaaaag aacatataat atacccatta tcaattaact
     4441 taatttcaga aggaagagta agtatttctg gaagaaaagt acttataaaa taaaaatccc
     4501 cctttttccc cgttgtcaaa cgggaaagat taaacgaaaa gaggtaaaaa tgcacactaa
     4561 ccaagatata aaaataagaa aagcaacaat aaaggacaca aaacatatta aaaaaattgt
     4621 agataaattt gctgatgagg aaaaaatgct tcctttatct tataatcaga tatacgaaag
     4681 aattagggat ttctgggtaa ttgaatcagg aaaacaaatt attggttgtg ggtctttaaa
     4741 accaacatgg aaagaccttg ctgaaataag gtcgcttgca gtaaaagaga attttcaaaa
     4801 aatgggatat ggaaaattgt taattgaaaa tatattaaag gaaggggaac aattgggctt
     4861 taaaaaaatt tttgttctta catataagcc aggatatttt gaaaaatttg gatttaaaaa
     4921 aatatcaaaa tcaaagttgc cacataaaat ctggtttgat tgtataaatt gcccaaaatt
     4981 cccaaaatgt gatgaaacag cgttaataaa agaacttata aaaaaatgaa aaaattaata
     5041 ttaataattt ttcttctatt ctttccttct attcttgaaa gtaaagtttt cttcggagaa
     5101 aatataaaaa tagaaagtat aaaagaagac atttttgctc ttggggagaa aatagaagca
     5161 aaagggaatc ttgaaaatga atttattgga attggtagaa atattctttc tgacatttct
     5221 ttaaatggag attatattgg aattgggtta aatcagaagt taaaatgtga aagtaaaagc
     5281 gatgtttata tgttaggaaa taatgttgaa acgacaggaa atataaaagg gagtatagca
     5341 atatttggta gtattatttc actaaaaggt aatatagaaa aaaatttaag gttatttgga
     5401 gaaaaaatct ttcttgatac aaatgtagat ggaaatactg taatatgggg acagaatatt
     5461 tatcttaaag gaaattttgg gaatttagta attcatagtg acgaaataaa ttttaatgaa
     5521 atgtcagtta taaatggaaa tcttgtttat tattccaaaa aagaaatgga ttttaaaaat
     5581 gtaaaaatta aaggcgaaaa aatctggaaa aaaccaatga gtgagacaat aaaagaaaaa
     5641 atgcctattt tgaaactaaa aagtttttat aatttccttt cacttttatt ccctttcttt
     5701 tttatgctct ggttcactcc caatgttctt tctcagacag caatttattc agggaaaaaa
     5761 tttttatatt gttttggact tggtattatt ttaatattga ttactttaat aattgttccc
     5821 attatatttg taacaataat tggcatccct tttggtctta ttgtaagttc tatatttatt
     5881 tcttgtgtat atgtctcaag ggtttttcca ctaatttttg ttggaagaaa aatacttttt
     5941 aaacttcctg ataaaagaat aacatggttt ttttcaactt ttattggtgt tttatttttt
     6001 actttaattt caataatccc gaatttaaaa cttttactta atttaattac tatacctgtt
     6061 ggttttggag caattgtttt aggaagagtt gaccaattta aaaaactaaa aaaagcaaat
     6121 tttttataaa aaggaggcaa aaatgaaaat aaaaattatt tccaacttaa taaaggaagt
     6181 taatgaaaaa aatttatcaa ataatctatt ttatcttgca aaaaaccctc tcccttttag
     6241 aaaattaaat tatactatac caggacattc taaaaataca ctatatgaaa cagatgattt
     6301 tatagaaaat gaacttgcaa aatatggcta taaaattgaa aaagaagaag ggaaagttca
     6361 agcatacggc agggatataa caaaaccaaa aagttctcaa tatgctccac catccccaga
     6421 atcgccctgg tatactgctc ataatttata tgcaaaaaag aaaggtaaaa cttatcctga
     6481 tgaaattatt ttaattgttt cccataagga ctcccaaagt tgggtggatt ctcctggtgc
     6541 ttatgataat gctgttggag tagtaagtaa tatggaaatt gcaagaatac tttcaaatta
     6601 cgaatctgtt cgttcactat atttcctatt ttgtaatgaa gaacatacac catggacaag
     6661 tataactgct gcaaaaaacg ccaaaacacg aggagataat ttgataatga taatcaatat
     6721 tgatagtatt ggtggtaaat caaaagaaga ggctgaaaca ggggcaaaaa caaatgtaac
     6781 tctttacaca actgatgaag gagaaaaaat tgctgacctt atgatagaat taaataaaat
     6841 atataatatt ggactaattc aaagaaaata taaaagaacg tctcctgggg atgatgatgg
     6901 ttcatttatc aaagaaggat ataaggtagc agtagcaaat attggctctt atccttatgc
     6961 ggacccaaat tatcatgatg aaaaagatat tcctgaattt gtggatatac aaaatgtaaa
     7021 aatgtcaact caagcaatat tatcttcaat tttaacaatt gacaaggagg gaataaaatg
     7081 aaaatagttg ttgcatattc tggtggactt gatacatcta taataataaa atatctgata
     7141 gaaaaatata atgcagaagt tattgctttt actgcaaaca taggacaaaa aataaaagaa
     7201 aaagaacttg aaaaaaaagc actaaaaaca ggagcaaaaa aattttattt taaggattta
     7261 agggaaaaat ttgttaaaaa ttacatcatc ccatctctaa aaggttctgc tctttaccag
     7321 gacaaatatc caatggcaac ttcattatca agacctttga ttgcagaaga aatggtagaa
     7381 atagcaaaaa aagaaaaagt tgatgcagtt gcacatggtt gcacagggaa aggaaatgac
     7441 caggtaagat ttgaacttac atttaaatac ttatctcctg aacttaaaat aattgctcct
     7501 ttaagagaat gggattttaa atccagagaa gaagaaattg aatatgcaaa aaagaataaa
     7561 atcccaattc ctgtctcaaa agaaaaacca tatagtattg atgagaattt atggggtata
     7621 tcaatagagt gtggaaaatt agaagaccca tgggaacaac caccagatga tgcatatcaa
     7681 attaccatat ctcctgaaaa agcacctgat aaaccaacat atattgaaat tgaatttgaa
     7741 aaaggagttc ctgttggtct caatgggaaa aagatggata ttttgaaaat catagaattt
     7801 cttaataaag ttggtggtga aaatggagtt ggtagaattg atatggttga agatagagtt
     7861 gttggtatca aatcaagaga gatatatgaa gcaccagcag cagttatttt acatacatca
     7921 catagtgaac tggaaaaact tattttcaca aaagaattat gggaatttaa aaaacttgtt
     7981 tcaattaaat tttcacaact tatttattac ggaaattggt tttctgattt tagagaatgt
     8041 ttaaccgcat ttgttgaaga gacacaaaaa tatgtaacag gaaaagtaaa agtaaagtta
     8101 tacaaaggga attgtgaggt tgttggtaga aagtcccctt actcacttta tattgaagaa
     8161 cttgcaactt atactgaaaa agatattttt gaccataaag catcaaacgg ttttattgaa
     8221 atttttggac tttctgcttt acttgaagga aggagaaaat taaaaactaa aaaagagaaa
     8281 taaaatgctg atttttaaaa gatttgtgcc tctgttctgg ttaagataga agaacatggt
     8341 tctctaggag aaccatgttc ttgaaaagag tattttgttt ctgcctatct tttcaggaca
     8401 tgggcttaaa atcgttatgc cgtcaagacc atatcctaat tgcaggaatt ttaaaggttt
     8461 ttctttctac aaagaaaatg cgattgaggg ctgcgacttt tagccagaga tgaaaatcgc
     8521 atcaatatat ttttgtttgg taaaaatata ttgatataat aatagcaaaa gatatttccc
     8581 ctcactccgc cctctcccct ccaccacaaa gattggcgga cagaattagg ggagaggctc
     8641 gcccgtaggg ttcctcgctt ttggctacgg attatgaact acgcccgagg gattaaagtg
     8701 aggggagaat taaagtaaga caataaattt gctgtcaaat ttgtataagg tggtggtaag
     8761 acaatataga gaaacacagt agttcatatg aacaaccgag gctatgggag atttaaagaa
     8821 gccccgacct ttggtcggcg ttcttcactt tccctttatg acgaggtgtt cctattctac
     8881 aaggtgatac taaaaaacca tagtgctcgg caaattctaa ataagacctt tgtaataccg
     8941 tatcatacac agctgctttt acaaccgccg cctttaagtt gtctaatact actttcttta
     9001 ccactccctc aaaatattca aacccatcct gctgtaacct taaccatgta cttatcttct
     9061 ggtcaaaaac aaacttaaca aatatatgtc tactataact taataccatc ccaaaacacc
     9121 acgccttccg caaccttttc tccacaaaat catacatcaa tccactatat ccaaaatcta
     9181 cctgtgcctc ttctcccggt gctgtctcta tacgaaagta tttatcagac tctttttccc
     9241 ttatattctt tatatatcgc ctcacagaat cataactccc tttaaatcca tagttgtctc
     9301 ttaatcgttc gtatatcacc tgtatactac aatttttact tattaattct tctaccctgt
     9361 cgtgatatgg aactactttg gaaccttttc tttctcctat cccaatcata cctgattctt
     9421 ctaaaaattt ctctattatc tccaaagaag gtagttcacc ttccaataaa ttatgccagt
     9481 tagcccattg tctgtatttt gataccgtcc tcctgttaat tcccaacatc tttgctatcc
     9541 gacgattact ctctttcatc cttaaacatc ttaacatctc tcttatatct atcactttta
     9601 ttctccttcc tgccataata tacctccttt caggtcttta ggatgtttcc taaaaggtta
     9661 catatttaga cactttttac aatctttttc tccctattcg acctggcgca ctatgagtgc
     9721 cgatagttag tacattatac cctgcttggt gacaccttcc ttcctctgtt atctttatct
     9781 cccctttctt ttctaatatc ggtactatct ctcctatata atctcctaat ctcctgccac
     9841 atggacaatc taatatgtac catacttctt ttaatgcttt ttcca
//
LOCUS       DAOOVW010000057         9764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_4403_length_9764_cov_8.809558, whole genome shotgun sequence.
ACCESSION   DAOOVW010000057 DAOOVW010000000
VERSION     DAOOVW010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9764
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4403_length_9764_cov_8.809558"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PLW95_07170"
     CDS             complement(<1..362)
                     /locus_tag="PLW95_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOV22434.1"
                     /translation="MVRIKIFPSLVKRGEGRFKIHPLGRSPEKFCGPYGRAHLPLQKE
                     GVGKRKASISASAVSCGGGFTLIELLVVIAIIAILAAMLLPVLSQAREKAKGSICMNN
                     LKQCGMAIFMYTNDYNEY"
     gene            complement(661..1653)
                     /locus_tag="PLW95_07175"
     CDS             complement(661..1653)
                     /locus_tag="PLW95_07175"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOV22435.1"
                     /translation="MKTKAVVFTRENEIELKELTLEDIGKDDIGVRTIITAISPGTER
                     WILKGKHIGTKFPCVPGYHRIGVVEKVGENVKNFKEGDVVYGCAGDGKWKEKEIYSMW
                     GAHLSYSIGDWRNYLFLSSKIPDKFELETNVFTQLSSVATRGINALEIKPQEKIIIIG
                     SGILGICASQLASYKNAIPVLIDKDTERVEFSKKFAFSFNIDDENLENKLKEIAPDGF
                     DYLYDTVGFPETTDKLVQFMKYRGKVLFQAQYFDKERCAVDLDQIKIKELTIKTTCGI
                     DTENFYQTLFYIRKRILKISDIITHRFSSREILDGYKIFKEGRQFNLGILFWWD"
     gene            complement(1662..2699)
                     /locus_tag="PLW95_07180"
     CDS             complement(1662..2699)
                     /locus_tag="PLW95_07180"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOV22436.1"
                     /translation="MENKMKVAVSEGRGDIKLVEIDIPEIDDYQCLCKTLSCATCSGT
                     DLKIINSKMFWAKNYPVIVGHESVGQVIKKGKKVRYIEEGDIILRPTAVYAGQQTNGY
                     FSALGGFAEYGVITDLNAFKEDNPGKEFNNYCIYQLKLPSDIKISPADATMLITAKET
                     AGFLFNIGVKLNSSILILGSGAVSMNLCYFSKILGCFPVIIIGRNQKTLEYIKNLGAD
                     WTINTKQENFIKKVKEITEGKGVDFVIDTTGNKELVVNCLEVISEKGGIAPYASYEEE
                     DVFGEYKEDNRFIFKSPGECETHNYLCYLLRMNLIELRYFYSNVLPFSEIEYGFKLLK
                     EKKAFKIVFEM"
     gene            complement(2773..2952)
                     /locus_tag="PLW95_07185"
     CDS             complement(2773..2952)
                     /locus_tag="PLW95_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22437.1"
                     /translation="MRKNLPFDKTSSPLKNENVLFHTGFCKYVVKCSEQIKISLTHLF
                     TSVFIIAQNEMANLT"
     gene            complement(2956..4737)
                     /locus_tag="PLW95_07190"
     CDS             complement(2956..4737)
                     /locus_tag="PLW95_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22438.1"
                     /translation="MKRYFTPAITRSPLSDFYGREIEDSIANHPEWYLKEIRDEGFDS
                     VWLHCILREVVSSSPFPEFGNKDKLDCLKKFVEYTSKFGIKVYLYLCEPRGLVEKDKF
                     WEKHPDVKGQPHIWVGTDFSGKYYALCSSTQKVRDFLYESCYNLFKDVSGLGGIFCIT
                     ASEFHTHCYSHFPKWKKEVKNFPEMIEWARQGFYCKRCEKRQPYEVVSEIITLINKGV
                     KDANKNADVIAWSWSWNIIEPQPQKNLINSLPEDVILMGDFERGGYKFMKGKRYPVDE
                     YSLSYIGPSPRFKQVIVLAKNRGMRTMAKLQMSTTHEIVTIPYIPAIHNFAEKLDRMR
                     KMKIDGFLSCWIFGGNISPMSKIAGKFSTSEKSKKEIIKEVAISEFGNSAYKKVINGW
                     KEFSIAFSNYPFSVPFTYNGPINYAVVYPLSIDAKKVDAIPSWRPLPRDEKGHLKVGD
                     NLETFLDIPYETFCYQMEKLTKKWKEGLKILEQGMKKEDNKRLKREIDLANYVYLSFR
                     SSLNIVSFYVNLREYKKGKKEETIKIKKVLEDELEITKSAYEIFKRNPDFGYHPEAYE
                     NFLNEDDFKYKISLIQSQLKGLNKIEK"
     gene            complement(4691..6706)
                     /locus_tag="PLW95_07195"
     CDS             complement(4691..6706)
                     /locus_tag="PLW95_07195"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOV22439.1"
                     /translation="MHKPKRCLFFDFHTMPACPDVGEKFNPDEFTDRIKRCGVDFIVF
                     PAKCNLGMCYYDTEIGNKHPSLKYDMFGKLVEFCKKKGIAISAYINVGISHEDAIHHR
                     EWCKITPEGYIYKPDRLDSFFRLMCYNTPYKDYILEIVKEVVSKYPVDGLFLDCFAPN
                     SPCVCPDCIGEMKEKGIKWDDFEKQKEFVLFSAVRMAERISQTARNLNPELLLYFNGI
                     PYFLQQDIGNYLELECLPTGGWGYDYLPVYAKYLRNLGKPVLNMTGRFHKSWGDFGGI
                     RTEASVEYDVFLGIANTLRVTIGDHMHPRGEINKPVFDLAENIYKKVQKYEIYYENAK
                     PLVDTALIVPEEWMGFSDEDGLSIAKGATRMLSELKMQFDILTDIPEDNGKYKLLIFP
                     DNFLFDDEEEEKLKKYIKNGGKVLLSGYSGINKEKNKFVIEDFGLEFKGELEYDPSFV
                     IVEEKISKDIPDMPMNFYEGGIKIIVSDKAEVLARIVAPYYNRHWDGEHGFVYLPPDK
                     ITEYPAAIKNRDLIYVSHFVFKSYFHSSPIYLKNFVRNLIEILLPDPIVKTTLPSFAK
                     VLTTYQEKRTILHILTYIPERRGEVNIIEEPVEVTDIDMKLKLDNKEIKKVYDVSQGK
                     EYSFKIENNYVLIKVPYIKGYSIIVFEYKKEKNEKIFYTSNNKVAIK"
     gene            complement(6943..9447)
                     /locus_tag="PLW95_07200"
     CDS             complement(6943..9447)
                     /locus_tag="PLW95_07200"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046353 - aminoglycoside
                     3-N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HOV22440.1"
                     /translation="MNNKKLYSQIYEIIDEKYLFYNIERLWKVEFGQTFKDYHNAAEL
                     TARLIKEVGLQNVEKISFPADGKTSYQDKITPLAWDASYGKLTIIKSPVSLQDNVVAD
                     YKRHPFHLIKGSVSTPPEGLNVRLITWEQLTHGQDAYKTMVVLDPDILPRDEVVRYIL
                     DMGAIGWINTSLTGRYLTPDDICWMNACTEGNHWHVHSDDRPFIGFCVSPKVGDTLLS
                     ASRKGEVIAKVECDGRRHEGEVDVVTGVIPGEDKKEFWLISHLYEPLASDNSLGVITG
                     ICIAKVLKTLFDSGILPKPKFTLRLVSGLEMYGFAAYVEKRGGNLRNEVVGAMNLDGL
                     TCRKGENVNCWLSPKASPFFGNSIFEEMANNIPNEDLPDIKFEIKEEGAYFDDMFLAD
                     PTVGIPVIWTLKQAVVHHNSCQTMDFIQIPIVKQYSTFYAAFTANVLTTNPAKMSTLE
                     EPSISSIEKHHDINNTLWKIAETIIPERITRGFPFDLAKVPKEERRKLPDGCIYGPFA
                     RVLANMDGNKSLKYLIQEAEIESKQDIKEAKVREYIGAIEFLAEYGYLKNKYTLCINK
                     EDIIKSLKDVGIKNGNLVLVHSGLSHFGHIEGGADTVIDAFLDVIGKEGTLLLPTFTS
                     SYIYFEGECAPIEPIYRPFDKNSNIVNTGKIPSMFLKREGVIRSLHPSHSVAGIGPLA
                     NKCLVDHLPTDPPTCRRSPFGKLLENNGKMIWFGADLATTTFFHFLEDELNIPYLGKC
                     ICRMKKEDGTIESILIEKHLPGHRDFYRVPAEESKMYRKLIQEGLIIKKAVLGFGEIK
                     VIEAEQMYALGIKALKECPTLLLCDDKECRFCSRFV"
     gene            complement(9518..>9764)
                     /locus_tag="PLW95_07205"
     CDS             complement(9518..>9764)
                     /locus_tag="PLW95_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22441.1"
                     /translation="WWPLHPIGFLIGPVWLMDYLWFSIFIIWLIKKLILKYGGANIYE
                     KLKYFFLGLPLGLYTCAGVWFIIDFFTGKHGNLIFWI"
BASE COUNT         2987 a         1917 c         1148 g         3712 t
ORIGIN      
        1 aaatactcat tataatcgtt tgtatacata aaaattgcca tcccacattg ttttaagtta
       61 ttcatacaaa tagaaccttt tgctttctcc cttgcctgtg ataatacagg taataacatc
      121 gctgctaaaa tcgcaattat agctattacc acaagcaatt caatcaatgt gaaccctccg
      181 ccacaagaca cagcggacgc tgaaatactc gcttttcttt ttccaactcc ttccttttgt
      241 aaaggaaggt gggctcgccc gtagggtcca cagaatttct ctgggctacg cccgagggga
      301 tggattttaa atcttccctc acccctcttt actaaagagg ggaatatttt tattcttacc
      361 atcttacacc tccttccgcc acaagacatg gcggatgctt aaatacactt ttttgttttc
      421 catccaccaa atagatggta gatgcttaaa tacaccccct cttttttcca tctcttcact
      481 tctttaaaat ctcctctcac cccttccgcc acaaatcctg tcggacagat actttgttaa
      541 agaggggagt ttttttgttt ctatcttttc ctcacctcct tttcttaatt gtctaacttt
      601 attatacact ataaaaaaat tttaccaaga ggcaaatttt cctcccttta ttttctgaat
      661 ttagtcccac cagaaaagaa ttcccaaatt aaattgtcta ccttctttaa aaattttata
      721 tccatctaat atctctctac tactaaatct gtgagttatg atatcggaaa tttttaaaat
      781 tctttttctt atataaaaaa gtgtttgata aaagttttct gtatctattc cacatgttgt
      841 ctttattgtt aattctttta tttttatctg gtcaaggtct actgcacatc tttctttatc
      901 aaaatattgt gcctgaaata aaactttccc tctgtatttc ataaattgaa ctaatttatc
      961 tgttgtctct ggaaatccaa ctgtatcata taggtagtca aatccatcag gagcaatttc
     1021 ttttaatttg ttttctaaat tttcatcatc tatattaaaa gaaaaagcaa atttcttact
     1081 aaattcaact ctctcagtat ctttatctat caaaactggt attgcatttt tataacttgc
     1141 aagttgagat gcacatatcc ctaaaattcc acttcctatt ataataattt tttcttgtgg
     1201 ttttatttca agtgcattta ttcctcttgt tgcaactgat gataattgag taaaaacatt
     1261 tgtttcaagt tcaaatttat ctggaatttt tgaagataga aacaggtagt ttctccaatc
     1321 tccaatagaa taggaaagat gtgctcccca catactataa atttcttttt ctttccattt
     1381 cccatctcct gcacatccat atacaacatc tccttctttg aaatttttga cattttctcc
     1441 tactttttcc actactccta ttctatgata acccggtaca catggaaact ttgtccctat
     1501 atgttttcct tttaatatcc atctctctgt cccaggagat attgctgtta ttattgttct
     1561 aacacctata tcatctttcc caatgtcttc aagtgttaat tcctttaatt caatttcatt
     1621 ttctcttgta aaaaccactg cttttgtctt cattattcac ctcacatttc aaaaacaatt
     1681 ttaaatgctt ttttttcttt taatagtttg aaaccatatt caatttcaga aaaaggtaaa
     1741 acattggaat aaaaatacct caattcaatt aaattcattc ttaaaagata acacaaataa
     1801 ttgtgtgttt cacactcacc aggggattta aaaataaatc tattatcctc tttatattca
     1861 ccaaatacat cttcttcttc ataacttgca taaggagcaa taccaccttt ttctgatata
     1921 acttctaaac aattaactac caattcctta ttgccagttg tatcaataac aaaatcaact
     1981 cctttccctt ctgttatttc ttttactttt tttataaagt tctcttgttt tgtatttatt
     2041 gtccaatctg cccctaaatt ttttatatat tcaagtgttt tttgatttct gccaataatt
     2101 attacaggaa aacaacctaa aatttttgaa aaataacata aattcatact gactgcccct
     2161 gaaccaagaa ttaaaattga tgaatttagt ttaactccaa tgttaaataa aaaacctgct
     2221 gtctcttttg cagttataag cattgttgca tctgctggtg aaatttttat atctgatggt
     2281 aattttaatt gataaataca atagttatta aattctttac ctggattgtc ttctttaaat
     2341 gcatttaaat cagtaataac tccatattca gcaaaaccac caagggctga aaaatatcca
     2401 tttgtttgct gacccgcata tacagcagtt ggtcttaaaa ttatatcacc ttcttctata
     2461 tatcttactt tctttccttt tttaatcact tgcccaacac tttcatgtcc aacaataact
     2521 ggataatttt tagcccagaa cattttactg tttattattt ttaagtcggt accagaacat
     2581 gttgcgcaag atagtgtttt acataaacat tgataatcat ctatctcagg aatgtcaatt
     2641 tctaccaatt ttatatcccc tctgccttca ctaacagcaa ctttcatttt attttccatt
     2701 ttctttttcc tcctctctgc ctttttattt ttttctatgt ctaactttac tatacaacat
     2761 aagaaaattt tgtcaagtca agttcgccat ttcattttgg gcaattatga aaacacttgt
     2821 aaataaatgg gttaaagaga ttttaatttg ttcactacat ttcactacat atttgcagaa
     2881 acccgtatga aataaaacat tttcattttt gagtggcgaa cttgtcttgt caaaaggtaa
     2941 attttttctc attttttatt tttcaatttt atttaaacct tttagttgag attgaattaa
     3001 tgaaattttg tacttaaagt catcttcatt taaaaaattc tcatatgcct ctggatgata
     3061 tccaaaatca ggattccttt taaaaatctc ataagcactt tttgtaattt caagttcgtc
     3121 ttctaatacc ttttttattt ttattgtttc ttcttttttc ccctttttat attccctcaa
     3181 attcacataa aaactcacta tatttagaga acttctaaaa cttaaatata catagttggc
     3241 aaggtcaatc tctctcttta atcttttgtt gtcttctttt ttcataccct gttcaagaat
     3301 ttttagtccc tctttccatt tctttgttaa cttttccatc tgataacaaa atgtctcata
     3361 gggaatatct aaaaatgtct ctaaattatc acctactttt aaatgccctt tttcatctct
     3421 tggtaatggt ctccatgacg ggatagcatc aacttttttt gcatcaattg ataatggata
     3481 gacaactgcg taatttatag gaccattgta tgtaaaaggt acagaaaagg gatagttaga
     3541 aaatgcaatt gaaaattcct tccatccatt tattactttt ttataggcac tattaccaaa
     3601 ttctgaaatt gctacttctt ttattatttc ttttttgctt ttttcactgg ttgaaaattt
     3661 tcctgcaatt ttactcattg gtgatatatt tccaccaaat atccagcaac ttaaaaatcc
     3721 atctattttc atcttcctca ttctatcaag tttttctgca aaattatgga tagcaggaat
     3781 atatggaata gttacaattt catgagtggt tgacatctgt aatttcgcca ttgttctcat
     3841 acctctattt ttagcaagaa ctattacctg tttgaatctt ggtgaaggtc ctatataaga
     3901 tagagaatat tcatcaacag gatatctttt ccctttcata aatttatatc cacctctttc
     3961 aaaatccccc attaaaatta catcttctgg aagggaattt attaagtttt tttgtggttg
     4021 tggttcaatt atgttccaac tccatgacca ggcaattaca tctgcatttt tgtttgcatc
     4081 tttaactcct ttatttataa gggttattat ttccgaaaca acttcatatg gttgcctttt
     4141 ttcacatctc ttacaataaa aaccctgtct tgcccattct atcatttcag gaaaattctt
     4201 tacctctttt ttccattttg gaaaatgact ataacaatgt gtatgaaatt cacttgcagt
     4261 tatacaaaaa atacctccaa gtccagaaac atctttaaat aaattgtagc aactttcata
     4321 taagaagtcc cttacttttt gtgttgatga acataatgca taatatttgc ctgaaaaatc
     4381 agttccaacc cagatatgtg gttgtccttt tacatcagga tgtttttccc agaatttgtc
     4441 tttttctact aatccccttg gttcacataa atataaataa acttttatcc caaatttaga
     4501 tgtgtattca acaaatttct ttaaacaatc taatttatct ttatttccaa actctggaaa
     4561 aggacttgat gaaacaactt ctcttaaaat acaatgcaac cacacagaat caaatccctc
     4621 atcccttatc tcttttaaat accattcagg atggttggca attgagtcct ctatttctct
     4681 tccataaaaa tcacttaatg gcgaccttgt tattgctggt gtaaaatatc ttttcatttt
     4741 tctccttttt atattcaaaa acaattatac tatatccctt aatataaggt acttttatta
     4801 atacataatt attttcaatt ttaaaactat attctttgcc ttgtgaaaca tcatagacct
     4861 tttttatttc tttattgtct aattttaatt tcatatcaat atcagttact tcaactggct
     4921 cttcaattat atttacttct cctcttctct ctggaatata tgtcaaaata tgtaaaattg
     4981 ttcttttctc ctggtatgtt gtaagaactt ttgcaaaaga tggaagtgtc gtttttacga
     5041 ttgggtctgg aagtaaaatc tctattaaat tccttacaaa atttttaaga taaataggag
     5101 aactatgaaa atatgattta aaaacaaaat gacttacata tatcaaatcc ctatttttta
     5161 ttgctgctgg atattcagta attttatcag gtggtaaata aacaaaccca tgttcaccat
     5221 cccaatgtct gttgtaataa ggagcaacta ttcttgctaa aacttctgct ttgtcagaaa
     5281 cgattatttt tattcctcct tcataaaaat tcataggcat atcaggtata tcttttgata
     5341 ttttttcttc aacaataaca aatgatgggt catattcaag ttctccttta aactctaatc
     5401 caaaatcttc aatgacaaat ttatttttct ctttgttaat ccctgaatat ccagataaaa
     5461 gaacttttcc accatttttt atatattttt ttaatttctc ttcctcctca tcatcaaaaa
     5521 gaaaattgtc agggaaaatc aaaagtttat atttcccatt atcctctggt atatctgtta
     5581 gtatatcaaa ttgcattttt aattccgata gcatccttgt tgcccctttg gcaattgata
     5641 aaccatcttc atctgaaaaa cccatccact cttctggaac aattaaagca gtatcaacaa
     5701 gcggttttgc attttcataa tatatttcat atttttgtac ttttttatat atattttctg
     5761 ctaaatcaaa aactggttta tttatttctc ctcttggatg catatggtct ccaattgtaa
     5821 ctcttaatgt atttgcaatc cctaaaaaaa catcatattc tactgatgct tctgttctta
     5881 ttcctccaaa gtccccccat gatttatgaa atcttcctgt catatttaaa actggcttgc
     5941 ctaaattcct taaatacttt gcataaacag gtaaataatc atatccccat ccacctgttg
     6001 gtagacattc taattccagg taatttccta tatcctgttg taaaaaatat gggataccat
     6061 tgaagtaaag aagaagttct ggatttaaat tccttgctgt ttgggaaatc ctctctgcca
     6121 ttctcacagc agaaaacaaa acaaactctt tctgtttctc aaaatcatcc cattttatac
     6181 ctttctcttt catctcacca atacaatcag gacaaacaca gggagaattt ggagcaaaac
     6241 aatcaagaaa aagtccatca actggatatt ttgaaacaac ctcttttact atttctaaaa
     6301 tatagtcctt atagggagtg ttataacaca ttagtcggaa aaaactatct aacctatctg
     6361 gtttgtaaat atatccttct ggtgttatct tacaccattc tctatgatgt attgcatctt
     6421 cgtggcttat acctacattt atatatgcag aaattgcaat ccctttcttt ttacaaaatt
     6481 caacaagttt cccaaacata tcatatttta aggatggatg tttattccct atttctgtat
     6541 cataataaca catacctaaa ttacatttgg caggaaaaac tataaaatca actccacacc
     6601 tttttatcct atctgtaaac tcatctggat tgaatttttc tccaacatct ggacacgctg
     6661 gcattgtatg aaaatcaaaa aaaagacatc tctttggttt atgcattttt ccccctatgg
     6721 gtaaaagttg ttttgctaaa aaagaaaaat tttaaattgt aattgtaatt ttcatccctc
     6781 caaaaagtca tttaacaaca tggtaaatcc gacgctcctt ttggcaaaag taatgggact
     6841 atctccatct tctttttgga ggacactgaa aaacacgctt tgtatttttc agtcatctcg
     6901 tatccgctgg gtatttacta ttttattatt atctctcata aattaaacaa acctactaca
     6961 aaacctacat tctttgtcat cacacaacaa aagcgttgga cactctttta atgcttttat
     7021 acccagggca tacatttgtt ctgcctcaat tacctttatc tctccaaaac ctaaaactgc
     7081 ttttttaata attagtcctt cttgtattaa ctttctgtac atttttgatt cttcagccgg
     7141 tacacgataa aaatctctat gacctggtag atgtttttct atcaaaatac tctctatagt
     7201 cccatcttct tttttcattc tacaaataca tttcccaaga tatggtatgt ttaattcatc
     7261 ttctaaaaaa tgaaaaaatg tcgttgttgc aaggtcagcc ccaaaccata tcatcttccc
     7321 attattttca agtagtttcc caaaaggact ccttctacaa gttggtgggt ctgttggaag
     7381 atggtcaacc aaacatttat ttgcaagcgg acctattcca gcaactgaat gtgatggatg
     7441 taaacttctt ataacacctt ctcttttcag aaacattgat ggaattttac ctgtatttac
     7501 aatattgctg tttttatcaa aaggacggta tattggttct attggagcgc attctccttc
     7561 aaaatagata taagaacttg taaaagtcgg taaaagtaga gttccttctt tacctataac
     7621 atctaaaaac gcatcaatta ctgtatctgc cccaccttct atatgcccaa aatgagacag
     7681 cccactatga actaaaacta aatttccatt tttaattcct acatctttca atgatttaat
     7741 aatatcctct ttatttatac ataaggtata cttattttta agatagccat attcagcaag
     7801 aaattctatt gcccctatat attctcttac ttttgcctct tttatatcct gttttgattc
     7861 tatttctgct tcttgtataa gatatttcag gcttttattt ccatccatat tagccagaac
     7921 tcttgcaaaa ggaccatata tacaaccatc aggtagtttc cttctttctt ccttagggac
     7981 ctttgctaag tcaaaaggaa aacctcttgt tattctttca ggtattattg tttctgctat
     8041 tttccacaat gtattgttta tatcatgatg tttttcaatt gatgatattg atggttcttc
     8101 aagagtactc atttttgcag ggttagtagt caaaacattg gcagtaaatg ctgcataaaa
     8161 agttgaatac tgtttaacaa ttggtatttg tataaaatcc attgtttgac atgaattatg
     8221 atgtactact gcttgcttaa gtgtccatat aactggaata ccaacggtag ggtctgctaa
     8281 aaacatatca tcaaaatatg caccttcttc ttttatttca aattttatat ctggcaggtc
     8341 ttcatttgga atattatttg ccatttcttc aaaaatggaa ttaccaaaaa aaggagatgc
     8401 ttttggactt aaccaacaat ttacattctc accttttcta caagtaagtc catctaaatt
     8461 cattgcacct actacctcat ttcttaaatt tccacctctt ttttctacat aagcagcgaa
     8521 tccatacatc tcaagcccag aaactaatct gagcgtaaat tttggttttg gtaaaatacc
     8581 tgaatcaaaa agtgttttta agacctttgc aatacatatc cctgtaataa cacctaatga
     8641 attatcactt gccaggggct cataaagatg acttattagc cagaattctt ttttatcttc
     8701 accaggtatt acccctgtta ctacatctac ttccccttca tgtcttctac catcacattc
     8761 aacttttgct atgacttcac cttttcttga tgctgaaaga agtgtatctc ctacttttgg
     8821 acttacacaa aaaccaataa atggtctatc atctgaatga acatgccagt gatttccttc
     8881 tgtacaggca ttcatccagc atatatcatc aggtgttaaa tatcgccctg taagagaagt
     8941 attaatccat ccaattgctc ccatatctaa tatgtatctt actacttcat ctcttggtag
     9001 aatatctggg tcaagaacaa ccattgtttt ataagcatcc tgaccatgtg taagttgttc
     9061 ccatgtaata agacgaacat tcaatccttc tggtggagta ctaactgaac cttttattag
     9121 atgaaatgga tgccttttat aatcagcaac aacattatct tgcaaagaaa caggtgactt
     9181 gattattgtc aatttcccat atgaagcatc ccaggcaagt ggagttattt tatcctgata
     9241 acttgttttt ccatcagcag gaaaagatat tttttccaca ttttgtagac caacttcttt
     9301 tatcaggcga gcagtgagtt ctgctgcatt atgatagtcc ttaaatgtct gtccaaattc
     9361 aaccttccag agtctttcaa tattataaaa aagatatttt tcatctatta tttcatatat
     9421 ttgactatat agtttcttgt tattcatctt tcactttctc ctcgtataat atgttcgtaa
     9481 atgttttaaa aaaccttgat aatttttctt ttctcattta tatccagaaa attagatttc
     9541 cgtgttttcc tgtaaagaag tcaattataa accatacacc ggcgcatgtg taaagaccca
     9601 atggcaaccc taagaagaag tactttaact tttcataaat atttgctcca ccatacttta
     9661 aaattagttt ctttataagc cagataataa aaattgaaaa ccataaataa tccataagcc
     9721 atacaggccc tattaaaaaa ccaattggat gtaatggcca ccat
//
LOCUS       DAOOVW010000058         9448 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_4568_length_9448_cov_7.352709, whole genome shotgun sequence.
ACCESSION   DAOOVW010000058 DAOOVW010000000
VERSION     DAOOVW010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9448
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4568_length_9448_cov_7.352709"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="PLW95_07210"
     CDS             <1..818
                     /locus_tag="PLW95_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22442.1"
                     /translation="HTTNSDGTLTPDEICKIYKNAGYDFISITDHNKFTMPSDSHNLL
                     LIPGIELTSNRKGEFHLVCIGIAKDIDVKGFFPQQMINTVNFKKGIPIIAHPYWSGLT
                     SSYVLKLSGWFGIEIYNNTCERRWGKGSSTIHWDEILQKGRKCFGFAADDSHHRFGEF
                     LEDDIAGSFIMVRASKLEEDEILNSIRNGFFYSSIGPLIQNFEVYKKKIYIKTTPVYT
                     INFIGYGWTGQLFSGKGKEITEIEYTIKGEEKYIRVEITDKNNKKAWTNPVYL"
     gene            820..1854
                     /locus_tag="PLW95_07215"
     CDS             820..1854
                     /locus_tag="PLW95_07215"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOV22443.1"
                     /translation="MDETFHIQWHITNSCNLRCTHCYQENFTSEKDIYFEFLKGIFTN
                     IENFLKEKNKKLVLDLTGGEIFLYKDWKKIIKLVFSSEIVKKIGIITNGFFLNDSTMK
                     FLKNFPEIEIKISTEGIEKEIYEFYRGKGNFKKFIEVMEILKSINFNKTLMFTITENN
                     SEQMEKIFDFCRQYNFSKFIIERFIPLGNGKEMREDIVKIDTWCKIIKILFEKCGLEF
                     DIADILPYRGFMVEMKNEGNFLYGASCIVGRDGMAIMPEGDVYPCRRFPLKIGNLLQQ
                     KLSDIWENSSVLNLSRDKKNLKGICKGCIINDCFGCRALAYSLKNDFLEEDILCFLNW
                     KGGENVNGTK"
     gene            1841..2383
                     /locus_tag="PLW95_07220"
     CDS             1841..2383
                     /locus_tag="PLW95_07220"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HOV22444.1"
                     /translation="MVPSKVFLTKGVGKDKEELTSFERALRNAGIAQFNLVSISSIFP
                     PNCKLISKKEGLALLQPGQVVYTVISRNKTNEPHRLITSAVGIAIPKDKNQYGYLSEH
                     AGFGETEDKAGDYAEDLAATMLATILGLEFNPDSSYDEKKEIWKMSEGIVKTTSIVQS
                     AIGEKNGLWTTVVAVAVFIL"
     gene            2383..3270
                     /gene="speB"
                     /locus_tag="PLW95_07225"
     CDS             2383..3270
                     /gene="speB"
                     /locus_tag="PLW95_07225"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HOV22445.1"
                     /translation="MENNFLGIKNTSFKSSKFLILSIPVELTTSFGKGTQFAPENIIF
                     SSKQLEFFDEQFKIEPYKYGIKTLEPLQIDVKNSHSMLKKIENSIKKLIKERKIIISI
                     GGEHTITYGIIKGFKHFYENFNVLILDAHADLRDKYQNSKFSHACVSRRITEEDLKVH
                     ILGVRNMSKEEFLFTESNKENIKIFYSYQMKKTDWRKEIVEKLPSGKYYLSIDADFFD
                     PSLIPEVGTPEPGGFFWDETIEFFENFILRKDIELIGFDFVELSPKRLDSPSSFISAK
                     IIYKLIGLLSLNEDKNHRK"
     gene            3245..4099
                     /locus_tag="PLW95_07230"
     CDS             3245..4099
                     /locus_tag="PLW95_07230"
                     /inference="COORDINATES: protein motif:HMM:NF027889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide 5'-hydroxyl-kinase"
                     /protein_id="HOV22446.1"
                     /translation="MKIKIIGNRHFEILFQKKINKILLIGNVDTGKTTILKEIARKFI
                     FYSIPVTIIDCDIGQSFIGPPTTISLIKLKKNVYNFFPFPDRFYFTGAISPSSNIIGF
                     LTGIEKIEEVCKREKNEKILIDTTGYIKDKIAREIKIHKIEVFSPDLVLLLEKKDELF
                     FVRNFLRYSKIKFLTIKIPENFPVKTITEREENRSEKFRRYFRITDKIKININNISIK
                     KINLDEGISLNNPDGLLLSLRDRNLNDRVLGVISKKERDFLEVFYPKGKIDFEVKGIA
                     ISNIKIDF"
     gene            complement(4115..4199)
                     /locus_tag="PLW95_07235"
     tRNA            complement(4115..4199)
                     /locus_tag="PLW95_07235"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4164..4166),aa:Leu,seq:cag)
     gene            complement(4663..5505)
                     /locus_tag="PLW95_07240"
     CDS             complement(4663..5505)
                     /locus_tag="PLW95_07240"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22447.1"
                     /translation="MNIEDIKKFIINSKKFDKLGNSYIIYGGTKQERIEVATFLASSL
                     NCENFCCGKCDSCLKIKNRTHPDVKWIIPEKSLLSIDTVRWIKEDIFISPYLGKSKSY
                     IFQINFIKEEAANSFLKILEEPPSYSNIIILSDNINFFLPTIISRCHKLKLNYCLPEF
                     NEEMEKSGNKFNEIISTLEEKKYFDFFKLIDTFVKEKEREEIEGFLENISIILRDIYF
                     KKNGLSSNLLVNKNIFDKKVVEKIDIENIEKTIETKNRIRYNVNIKLALENLFFSFFI
                     NSSL"
     gene            complement(5486..6112)
                     /gene="tmk"
                     /locus_tag="PLW95_07245"
     CDS             complement(5486..6112)
                     /gene="tmk"
                     /locus_tag="PLW95_07245"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOV22448.1"
                     /translation="MKKGLIISFEGIDGCGKSTQAKLFEKYLKENGEKTILLYEPGGT
                     VIGEKIRKLLLNNKSNIDKWTELFLYLASRSQLLQEKIKKEKDSGSTVILDRYIYSTI
                     AYQGYGRGISEKLIMTIHKYFIDKYFPDITFVIDVKPESVISVLNKKKKDRIENESIQ
                     FQRKVREGYLKISKSLNNVNVIKRKTVQKTHMEIIETWEKFKDEYRRY"
     gene            complement(6112..6708)
                     /locus_tag="PLW95_07250"
     CDS             complement(6112..6708)
                     /locus_tag="PLW95_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22449.1"
                     /translation="MSPIFGKIIAVILISLSIILLILSEIVFITNRKKGWISFVLSLI
                     FTGISYYYFLLFFYQNIQCSSVSKKSINLPVVKVEEKITEDGKNIDIKPEETSLLVYI
                     EMDGAILEATSEDEIEIPKETKFRITKVEIKPERKHIKANFVGFVPESKNTTNDINFW
                     ISYKNIRKDKAIDKEKTKFELIIKSGNEILGKVYFKFI"
     gene            complement(6783..7988)
                     /locus_tag="PLW95_07255"
     CDS             complement(6783..7988)
                     /locus_tag="PLW95_07255"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOV22450.1"
                     /translation="MQKNIYGKKLLIFTWVLLTVVISEIILFTNAFIKRDVPIREIFI
                     DLIPFLIFLYIFLFLTYKLVEHYYLQLLIQKTKLEKLHTAFLKVSSSLKVNEILKSSL
                     ETIMNYFNANKGIFIIIDERVKKYSYSDILVINNFAGNRKNNFENYRMITFYPLSIDN
                     EKIKEYIEEYQLNNCNSVMIIPIFEKGIIVLGTYQIMNKKEEEEFESLKIIIDIFTEQ
                     LNTQLENAILHEEINQLAITDPLTNLFNRRYFVERLKDEFILAKRQGFPVSIMISDLD
                     NFKHYVDTYGHSNGDIILREVAKVVKLSLRESDVVCRFGGDEFAYILPFTSSSDAKVV
                     ATRVKNNVSEYNFLENIIKEEIYLTLSIGIATFPEHGENEEEILGKADNALFKAKNIG
                     KNKVVIYGE"
     gene            8086..8814
                     /locus_tag="PLW95_07260"
     CDS             8086..8814
                     /locus_tag="PLW95_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22451.1"
                     /translation="MKIKVLFIFFLMSSIVFSKVNYRIIIDRNIFSPKSIEAEKKVEY
                     PEVVRKFTPPEIDKIFKVAGTIIFEEDRGKNIVILEDTKTKSMSFRKIGDIINGFKIL
                     EIDENEVIFESDGEKYLLNEIGCECLTIPKEAIVFEVKMGALLEVIEKQSNLIEKIEV
                     KKIKKDGNFIGFEIGGIEKDSFLEEFGIQNKDIITGINGHLFENEDFYFDFYEAILRQ
                     EIKRVEIKFLRDGKHHIYIYHFIP"
BASE COUNT         3409 a         1243 c         1360 g         3436 t
ORIGIN      
        1 ttcatacaac aaattctgat gggactttaa ctcctgatga aatttgtaaa atttataaaa
       61 atgctggtta tgattttatt tcaataacag accataataa atttacaatg ccttctgact
      121 cacataattt acttttaata ccagggatag aattaacaag taatagaaaa ggagaatttc
      181 atcttgtgtg tattggaatt gcaaaagata ttgatgtaaa aggttttttc ccacaacaaa
      241 tgataaatac tgtcaatttc aaaaaaggaa tacctataat tgctcatcca tattggagtg
      301 gcctaacatc aagttatgtt ttaaaattat ctggttggtt tggaattgag atttataata
      361 atacctgtga gagaagatgg ggaaaaggtt cttcaactat tcactgggat gagattttac
      421 aaaaaggaag aaaatgtttt ggttttgctg ctgatgatag ccatcatcgt tttggagaat
      481 ttttagaaga tgacatagca ggaagtttta taatggtaag ggcaagcaaa ttagaagaag
      541 atgagatatt aaattcaata agaaatggtt ttttctattc ttcaataggt ccattgatac
      601 aaaattttga agtatataaa aaaaagattt atataaaaac aacacctgtt tataccatta
      661 attttattgg atatggatgg acaggacaac ttttttctgg caaaggtaag gaaataacag
      721 aaattgaata tacaattaaa ggagaagaaa aatatataag agttgaaata actgataaaa
      781 acaataaaaa agcatggaca aatcctgttt atctataaaa tggatgaaac atttcatatt
      841 caatggcata ttacaaattc ctgcaattta agatgcacac attgttatca ggaaaatttt
      901 acttctgaaa aagatattta ttttgaattt ttaaagggaa tttttaccaa tattgaaaat
      961 tttttaaaag agaaaaataa aaaacttgtt cttgacctga ctggtggaga aatttttctt
     1021 tataaggact ggaagaaaat tattaaattg gttttttctt cagaaattgt aaagaaaatt
     1081 ggcattataa caaatggttt tttcttaaat gattcaacaa tgaaattttt aaaaaatttt
     1141 cctgaaattg aaataaaaat atcaacagaa ggaattgaaa aagaaattta tgaattttat
     1201 agaggaaaag gaaattttaa aaaatttata gaagttatgg agatattgaa aagtataaat
     1261 tttaataaaa cacttatgtt tacaataacg gaaaataaca gtgagcagat ggaaaaaatt
     1321 tttgattttt gcagacaata taatttctct aaatttatta ttgaaagatt tattccattg
     1381 ggaaatggta aagaaatgag agaggatatt gtaaaaattg atacatggtg taaaataata
     1441 aaaattttat ttgaaaaatg tggtttagaa tttgatattg ctgatattct gccttataga
     1501 ggttttatgg ttgaaatgaa aaatgaagga aattttcttt atggtgcttc gtgcatagtt
     1561 ggcagagatg gaatggcaat aatgccagaa ggggatgttt atccttgtag aagatttcca
     1621 cttaaaatag gtaatttatt acaacaaaaa ttatctgata tatgggaaaa ttcatctgtt
     1681 ttaaatttat caagggataa gaaaaatttg aaaggtattt gtaaagggtg tataattaat
     1741 gattgctttg ggtgtagagc ccttgcatat agtttaaaaa atgatttttt agaagaagat
     1801 attttatgtt ttttaaattg gaaaggaggg gaaaatgtca atggtaccaa gtaaggtttt
     1861 tttaacaaaa ggggttggta aagataaaga ggaattaacg agttttgaga gagcattaag
     1921 aaatgctggt attgctcaat ttaatctcgt tagtatatca agtatatttc ctcctaattg
     1981 taaactgatt tctaaaaaag agggacttgc acttttgcaa ccaggacaag ttgtatatac
     2041 tgttataagc agaaacaaaa caaatgaacc gcatagattg ataacatcag cagtgggtat
     2101 tgctattcca aaagataaaa atcaatatgg gtatttatct gaacatgctg gattcggaga
     2161 gacagaagat aaagcagggg attatgcaga agacttagca gcaacaatgc ttgcaactat
     2221 tttaggactt gaatttaatc ctgattcttc ctatgatgaa aaaaaagaaa tatggaaaat
     2281 gtcagaagga atagttaaaa caacaagtat agttcaatca gcaatagggg agaaaaacgg
     2341 tctctggaca actgttgtgg cagttgccgt ttttattcta taatggaaaa caatttctta
     2401 ggtataaaaa atacaagttt taaatcatca aaatttctta ttttatctat ccctgttgaa
     2461 cttaccactt cttttggaaa agggacacaa tttgctcctg aaaatataat attttcttct
     2521 aaacaacttg agttttttga tgaacaattc aagatagaac catataaata tggaataaaa
     2581 acacttgaac cattacaaat tgatgtaaaa aattcccact caatgctgaa aaagatagaa
     2641 aacagcataa aaaaactcat taaagagagg aaaataataa tttcaatagg gggggagcat
     2701 actataactt atggaattat aaagggattt aaacattttt acgaaaattt taatgtcctt
     2761 attcttgatg ctcatgctga tttaagagat aaatatcaaa atagtaaatt ttctcatgct
     2821 tgtgtttcaa gaagaattac agaagaagat ttaaaggttc atattttagg agtaagaaac
     2881 atgagtaaag aagaattttt atttacagaa agtaataaag aaaatataaa gatattttat
     2941 tcttaccaga tgaaaaaaac agattggaga aaagaaatag ttgagaaatt accttccggg
     3001 aaatattatt taagtataga tgctgatttc tttgaccctt ctttgattcc agaagttgga
     3061 acacctgaac cagggggatt tttctgggat gaaacaattg aattttttga aaattttatt
     3121 ttaaggaaag atattgaatt gattggattt gactttgttg aactatctcc aaagagattg
     3181 gattcccctt cgtcttttat ttctgcaaaa ataatttata aactaattgg gcttttatct
     3241 ttaaatgaag ataaaaatca taggaaatag acactttgaa attctttttc agaagaaaat
     3301 aaataaaata cttctaattg ggaatgtgga taccggaaag accacgattt tgaaagaaat
     3361 tgcaagaaaa tttatttttt attcaattcc tgttaccata attgattgtg atatagggca
     3421 atcatttata ggacctccaa caactatatc tcttatcaaa ttaaagaaaa atgtttataa
     3481 ttttttccca tttcctgata gattttattt tacaggtgct atatctccat cttcaaatat
     3541 aattggtttt ctcacgggaa ttgaaaaaat agaagaagta tgtaaaagag aaaaaaatga
     3601 gaaaatcctt attgatacaa ctggctatat taaagataaa atagcaagag aaataaaaat
     3661 tcacaaaata gaagtttttt ctcctgacct tgttttatta cttgaaaaaa aagatgaact
     3721 attctttgtg agaaattttt taagatattc aaaaataaaa tttttaacaa ttaaaattcc
     3781 agaaaatttt cctgttaaaa caataactga aagagaagaa aacaggagcg aaaaatttag
     3841 gagatacttt cgcataacag ataaaataaa aataaatata aataatattt caataaaaaa
     3901 gataaattta gatgaaggaa tatctttaaa taatccagat ggtttgctat tatctttaag
     3961 agatagaaac ttaaatgata gagttcttgg agtaatttca aaaaaagaaa gggatttttt
     4021 agaagttttt tatccaaagg ggaaaattga ttttgaagtg aaaggaattg ctattagtaa
     4081 tataaaaata gatttttgat tttgagtgat aaaatgccga agaggggatt tgaaccccta
     4141 cccgctataa tgcggaccag gtcctgaacc tggcgcgtct gccgttccgc cacttcggct
     4201 ttactcttct tttacatttc tactgacgac ctatattctt acaaaatttc tctgcttatc
     4261 acagttgtgc tacaatttct tcttttttac ttctttctct ataattgctg tttacttctt
     4321 ttttacccaa atttccctgc tctcatccgc accagctcgg cgtaagcaag tcatagtaaa
     4381 gtattagggg aaaaacaacg ccgaggttcc gccacttcgg ctttactttt cttttacatt
     4441 tctactgacg acctatattc ttacaaaatt tctctgctta tcacagttgt gctacaattt
     4501 cttctttttt acttctttct ctataattgc tgtttacttc ttttttaccc aaatttccct
     4561 gctctcatcc gcaccagctc ggcgtaagca agtcatagta aagtattagg ggaaaaacaa
     4621 cgccgaggtt ccgccacttc ggcttttact ctttgaatag tattacaggg aagagttgat
     4681 aaaaaaagaa aaaaataaat tttcaagagc gagttttata tttacattat atcttattct
     4741 gttttttgtc tctattgttt tctcaatatt ttctatatca attttttcaa ccactttctt
     4801 atcaaaaata tttttattta ccaataaatt agatgataac ccattttttt taaaataaat
     4861 atctcttaaa ataatagaga tattttccaa aaaaccttca atttcttctc tttctttttc
     4921 tttcacaaaa gtatcaatta acttgaaaaa atcaaaatat tttttttctt ccaatgtaga
     4981 tataatttca ttaaatttat ttccgctttt ttccatttct tcattaaatt caggaagaca
     5041 ataatttaat tttaatttat ggcatctgga tattattgtt gggagaaaaa aatttatgtt
     5101 atcgctcaat ataataatat ttgaataaga aggtggttct tcaagaattt ttaaaaaaga
     5161 gtttgctgct tcttctttta taaaatttat ctggaaaata tatgactttg attttcctaa
     5221 atatgggctt atgaaaatat cttcttttat ccatctaact gtatctattg aaagcaaact
     5281 tttttcagga attatccatt ttacatcagg gtgtgttctg ttttttattt ttaaacaact
     5341 atcacatttt ccacaacaaa aattttcaca attcaaagaa gatgctaaaa aagtagcaac
     5401 ttcaattctt tcctgttttg ttcctccata aataatataa gagttaccta atttatcaaa
     5461 ttttttagag tttattataa attttttaat atcttctata ttcatcttta aatttttccc
     5521 atgtttctat aatttccata tgtgtttttt ggactgtttt tctttttata acattgacat
     5581 tatttaaact ttttgaaatt tttaaatatc cttctcttac ctttctttga aattggattg
     5641 attcattctc aattctatct ttcttttttt tatttaaaac agatataaca gattctggtt
     5701 taacatcaat aacaaaggtt atatcaggaa aatatttatc aataaagtat ttatggattg
     5761 tcattataag tttttccgat atacctcttc catatccctg atatgcaatt gttgagtaaa
     5821 tatatctatc aagaattaca gttgaaccac tatctttttc ctttttaatt ttttcctgga
     5881 gaagttggga cctagatgca aggtataaga aaagttcagt ccatttatca atattacttt
     5941 tattatttag tagtaacttc cttattttct ctccaattac agttccacct ggctcataaa
     6001 gcaaaattgt tttttctcca ttttccttta aatatttttc aaatagtttt gcctgtgtgc
     6061 ttttcccaca accatcaata ccttcaaaac ttataataag tccttttttc attatataaa
     6121 tttaaaatat acctttccga gaatctcgtt tccactttta attattaatt caaactttgt
     6181 tttttcctta tcaattgcct tatcctttct tatattctta taacttatcc agaagttaat
     6241 atcatttgta gtatttttgc tttctggaac aaatccaaca aaatttgctt taatatgttt
     6301 tctctccggc tttatttcaa cttttgttat cctaaatttt gtttcttttg gtatttcaat
     6361 ttcatcctct gaagttgctt ccaatattgc tccgtccatt tcaatataaa caagtaaaga
     6421 tgtttcttct ggttttatat caatattttt tccatcctca gtaatttttt cttcaacttt
     6481 tacaacaggc aaattgatgc ttttttttga tacagaagag cattggatat tttgataaaa
     6541 aaataataaa aagtaatagt atgaaatacc ggtaaatatt aaagaaagaa caaaagaaat
     6601 ccatcctttt ttcctattag ttataaaaac aatttctgac aaaataagca aaattattga
     6661 taaagatatt aaaataacag cgattatttt tccaaaaatt ggactcattt tttctccttg
     6721 gtttaattag ttataatttt taaaaacctt catttatatt ccatttctta ttttaactct
     6781 tttcattctc cgtaaataac caccttattt tttcctatat tttttgcttt aaataaagca
     6841 ttatctgctt tacctaaaat ttcttcttca ttttctccgt gttcggggaa tgttgcaatt
     6901 cctatactta aagttaaata aatttcttct tttattatat tttctaaaaa attgtattca
     6961 gaaacattat tttttactct tgttgcaaca acttttgcat cactacttga tgtaaatggc
     7021 aagatataag caaattcatc tccaccaaat ctacatacaa catctgactc tcttaaactt
     7081 aatttcacaa ctttggcaac ttcccttaaa ataatatccc catttgagtg tccatatgta
     7141 tcaacatagt gtttaaaatt atcaaggtcg gaaatcatta ttgaaacagg aaatccctgt
     7201 ctttttgcaa gaataaattc atcttttaat ctttcaacga aatatcttct attaaataaa
     7261 tttgttaaag ggtcggttat tgctaattga tttatttctt catgcaagat tgcattttca
     7321 agttgagtat tcaactgttc tgtaaaaatg tcaattataa tttttaatga ttcaaattct
     7381 tcttcctctt ttttattcat tatttgatat gtccctaata caataattcc tttttcaaaa
     7441 ataggaatta tcatgactga attacaatta tttaattgat actcttcaat atattccttt
     7501 attttttcat tatctataga taggggataa aatgttatca ttctgtaatt ttcaaaatta
     7561 ttttttctat ttccagcgaa attatttatt acaagaatat ctgaataact atattttttt
     7621 actctttcgt caataataat aaaaattcct ttatttgcat taaaataatt catgattgtt
     7681 tcaagagaag attttaggat ttcatttact ttaagacttg aacttacttt aagaaaagcg
     7741 gtatgcaatt tttcaagttt tgttttttgt ataagcaatt ggagataata atgttcaacc
     7801 aatttatatg ttaaaaaaag aaaaatataa aggaaaatca aaaaaggtat taaatcaata
     7861 aaaatttccc ttattggcac atctctcttt atgaaagcat ttgtaaaaag gataatttca
     7921 ctgattacaa cagtaaggag aacccatgta aatattaata actttttccc ataaatattt
     7981 ttttgcatat atttattatt ttactttttt tcaaaaaaaa gtaaaaatat gttttttatt
     8041 acccgaaaaa ttgacagaaa ctgaaagaaa gaataaaatt tcaatatgaa aattaaagtt
     8101 ttatttatat tctttctaat gagcagtatt gtattttcta aggttaatta caggataatt
     8161 atagatagaa atatattttc tccaaaaagt atagaagcag aaaaaaaagt tgaataccct
     8221 gaagttgtaa gaaaatttac tcctcctgaa attgataaaa tttttaaagt tgcaggcact
     8281 attatatttg aagaagatag agggaaaaat atagttattc tggaagatac aaaaacaaaa
     8341 agtatgagtt ttcgtaaaat tggtgatatt ataaatggtt ttaagatatt agagattgac
     8401 gaaaatgagg ttatatttga atctgatgga gagaaatatt tattgaacga aattgggtgt
     8461 gagtgcttaa ctattccaaa agaagcaatt gtttttgaag taaaaatggg cgctctttta
     8521 gaagttatag aaaagcagtc caatttaatt gaaaaaatag aagtaaaaaa aataaaaaaa
     8581 gatggtaatt ttattggttt tgaaataggc gggattgaaa aagattcatt tttagaagaa
     8641 tttggcattc aaaataaaga tataattaca ggaataaatg gacatttatt tgaaaatgag
     8701 gatttttatt ttgactttta tgaggcaata cttagacagg aaattaaaag agttgaaata
     8761 aaatttttaa gggatggaaa gcatcatatt tatatttatc attttattcc ttgatttaaa
     8821 ttttatgaaa agtaagttat tgattttcaa tgacttgtta acttcgtaag ttaacaaaaa
     8881 tattttatga ctatccattg acaattacac tttggtatag gtaatatatt tatgcggagt
     8941 ggatgagtgc cagttcaagt accgggtagt ggagttgaaa aatcccgggg cgtcagaatt
     9001 ccttcgggaa gtaatccagg tttctggagc gcctacttca ttgcaccgcc actccgcttt
     9061 ttaactctaa ttcataaatt cttacgacaa ttaaaaaaga tgtcctacta tttttcctac
     9121 atatcagcaa acattcaacc caaattccgc cataattagg cactaagtaa gtttattata
     9181 tcatatattc atcacagcaa cagggttttt taacaaaggt agatgctata cttaatgcct
     9241 atatgaacat tataagaaga aaatatgtct ttatcccaat ttgagtttat acatacagta
     9301 aaaacctttc ccctcaccta tcctctcccc ggtggggaga gggaaaaaag agaagagggg
     9361 agatgctcgc ccgtaggata tacgcccgag gggaagggtg aggggggtat taatttctat
     9421 tatacatttg atgtaaaaac agtatgaa
//
LOCUS       DAOOVW010000059         9195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_4707_length_9195_cov_8.756455, whole genome shotgun sequence.
ACCESSION   DAOOVW010000059 DAOOVW010000000
VERSION     DAOOVW010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9195
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4707_length_9195_cov_8.756455"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(195..992)
                     /locus_tag="PLW95_07265"
     CDS             complement(195..992)
                     /locus_tag="PLW95_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22452.1"
                     /translation="MIIDSNSYLGRWGLRTKGITKVEEFLKIMDKNKIDISVVTSTVA
                     LVTDTKEGNLWLIKEIFPYLNRFIPVACINPIWGLKETMEYFDKYPFKIARISPSLHN
                     YSLKDIVFLNPFMDLFKEENIPLYITVEVCYNFCSIANKMGFSPPIYPISEIINFAEQ
                     YSSQKIVICGYDTQLFSEVNNLLIPSFKKCKNLFIEISDILVAGVIEKLVKEIPNQIL
                     LGTGNAINEPSGSIYKVKFAKITDEEKEKILFRNAEKVFSSENVIKK"
     gene            complement(989..1711)
                     /locus_tag="PLW95_07270"
     CDS             complement(989..1711)
                     /locus_tag="PLW95_07270"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOV22453.1"
                     /translation="MIIDAHTCWNTYEMKGIRISEEEYISALDQFGIDMAMVCCPFYL
                     LTDVSLGNNRVLKLMKKYPSRIIGFSTLNPLFGKESIEELDKCIKEGMKGIKLHCDLS
                     QIPYNDPLTFPIVEKAIELNIPLFLHTGEDSIEEAKFISQKYPEATFIFAHIGNTKWK
                     QMSRFAKEQKNIILCLSGTIFEQGFLEEAVSNVGDERVIFGSDFVLVNPAINLGIIKN
                     SKLSEESKKKILGLNIKRTLKL"
     gene            complement(1715..2902)
                     /locus_tag="PLW95_07275"
     CDS             complement(1715..2902)
                     /locus_tag="PLW95_07275"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOV22454.1"
                     /translation="MGKNFRCGVSKVDITPSIGCEMAGMGRGHIAKSIHDNLYANILI
                     LDDGKEKFAIVTADLIGIDIEITEKIKELVKGKINLENFLITASHTHYGPNTRFILPY
                     PEKDISYIYQLCKKIANGICIALRNMKEAKIGMGRGAIKESVNRRINLPDGSHHYLYD
                     EPQLLKYAKGPVDDEVGVICIEDMNSKPIATFFNYTCHPICLPIDNLSISADYPGVAK
                     RLIEEDIGGVAFFSNGACGNIGPIRALEGFQRMGELGENLAIEVKRVLKNIKFTSDVK
                     LKVAKSFVELPIREEIVKRWDMNRRFRNKKTIISEIITFSVNNLVFIGIPGEAFVELG
                     LEIKKKAPFSDTYILYLTNDDLLYIPTKESYKEGGYEVSVAMLGEESYDIILNQTLKL
                     MEN"
     gene            complement(2957..3433)
                     /locus_tag="PLW95_07280"
     CDS             complement(2957..3433)
                     /locus_tag="PLW95_07280"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOV22455.1"
                     /translation="MRCRKFKKGDAKQVSELMIKAFKSFLGDKLENFNEFSPSVFEKK
                     SNFKDAFSERISFVVEDNKKIVGYISVTAGKNGLGSLEIVGVEPEYFSKGVGKILMKK
                     AEEFWASKKQRKISTCVSAHNKRALIYYIKNGFIPEGYRRDHFFVGVDEIILGRFL"
     gene            complement(3490..4230)
                     /locus_tag="PLW95_07285"
     CDS             complement(3490..4230)
                     /locus_tag="PLW95_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOV22456.1"
                     /translation="MKYEMMFPDQIRKAIKDNTPVVLPVGVIEYHSEHLVVGVDTLLI
                     IRALEIIEKEMDVVILPPFYYGAASYGVEPPENNGTIHIPGPSLFPFAKGLFNGLLRI
                     GFRNIHIFIHHQSENFVQGMPTDLAFKFAARQSIFEFIEANLGEGWWGKESSKDYYTQ
                     QEKGQDPFNWIKIHPFITEEIQKKFPIDHAGKQETSLMLTFCPDGVDMKKFDESKWYS
                     KTAKEASLEYIKEAREEILSDIKKILKN"
     gene            complement(4317..5474)
                     /locus_tag="PLW95_07290"
     CDS             complement(4317..5474)
                     /locus_tag="PLW95_07290"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOV22457.1"
                     /translation="MEIKIPDSEFEKRWKEVVEKVKAKNLDILIVQSNEADFANVRYL
                     TDYWPIFETSGVAISKNGGIALLIGPESETFAMNRSRVKKIYRILQYRESAEPEYPGM
                     KLDNFSIVFKEMVGEKVKKIGIAGYQIFPIPIYEAIKQAEPDAEIIKSDEIMVDMRIK
                     KSEVEIKMMKEAFKISEIALKEAIENMKPEMTELQLVGIIEESLYKNGAEYEGHPQYV
                     LAGKNSTNAIGRPSYTKLGKNNLIQLNIGARVAGYSSSVGRPVCIGKMSEEMKKLVSA
                     GLDMHKKTIDWIKSGVKAGDVAKKFFEYGEKIGVGKNILYGPCHGIGMIEVERPWMET
                     NSDYYLEENMTFQVDTFLYCDDFGLRWENGVIVKKEGAELLSSELMKIIEV"
     gene            complement(5452..6573)
                     /locus_tag="PLW95_07295"
     CDS             complement(5452..6573)
                     /locus_tag="PLW95_07295"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOV22458.1"
                     /translation="MGKIKSNSYVPLSKQIKEKLLEDIRKGRILPGEKIPSEEKLAEE
                     FGISRMTVREAIIELINENLLFRMPGKGTFLTQEFNNGQNTIKDLIVIKVPNLRNSFY
                     FQIISGIQKVLSREGIEFTIFSERDNPIEEKIYLRKILKEKRKGLFLISSYYTHTNRS
                     TVDKISKEIPIVIIDVEIPGISADIVMSDDYKGGFLITEHLIELGNKKILHLLGPVGD
                     SSADERRNGYINAIEKYKIKEKIIRFTKWSLEDGYFETKKYFLNYNADAIFACNDEVA
                     VGAYKALRELNLRVPEDISLVGYGNMEVGQILESPLTTVDQQAEKIGESAAELLMDKI
                     KGKRKFSDIKKVKIDTKLIIRKSCGIYEKGVKNGNKNTG"
     gene            complement(6771..6935)
                     /locus_tag="PLW95_07300"
     CDS             complement(6771..6935)
                     /locus_tag="PLW95_07300"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOV22459.1"
                     /translation="METKKDKSREKINEFILNNNSHLTEEILCKKFKVSRTPVREVLK
                     YLEQDGFIQK"
     gene            complement(7103..8206)
                     /locus_tag="PLW95_07305"
     CDS             complement(7103..8206)
                     /locus_tag="PLW95_07305"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOV22460.1"
                     /translation="MIKVKKSGIVYRNSKPHLKSLHAYFPSLVLLRDGDILVSFTIGS
                     AFDSVDCHPVYSRSSDGGKTWVFEGDLPVIKRDFISSTCRISLCADGEIIGFGAYFIR
                     NSDEQELLNPETNGFVPTELFLIRSKDKGFIWTKPEIIIPPIKGHPFEICSPVVALTS
                     NEWLIPTSTWKGWNGEDPIGMKAIVLRSYDKGKTWSEYSIVMDGSNKEIVYWEQKLIK
                     IANEQLLSVCWTHNLKDKKDMEIHYAISQDRAKTFSAPQSTGLKGQTCTPIYLEDNKI
                     LSLYRRTDKPGLWANISHLDGNKWINDEEVPLWGEHFSQLTRKQRNIVKEMNILKFGL
                     PTIIKLNNKEIFAAFWCVEECVAIIRWFHIEIT"
     gene            complement(8290..8982)
                     /locus_tag="PLW95_07310"
     CDS             complement(8290..8982)
                     /locus_tag="PLW95_07310"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOV22461.1"
                     /translation="MIKKIKEIDNCYFIKIDCFFFLFLPFFLFIFFTREEKEEFKNFK
                     NRKRKIQFISGRIVGKFGVSQYFGREKLRYKEIKINREQKGNAYCFLKNKKIYLSISH
                     TDGISVATCGDSKFIGIDIEKKNKNIKKIAKKFLSKEEIEKTKGISLITLWCVKEAVA
                     KALGTGFVFSPKDIFIQSTDGEKFKVKLKGKFLSLFPEFENKEIKVKTFKKYNYILAF
                     CNIEDNTFHLKK"
BASE COUNT         2863 a         1687 c         1010 g         3635 t
ORIGIN      
        1 caaaatatgc aatgaaaatt gaaagtaaaa gaccagaaat aaatgctctt cttgtaaaga
       61 atttatccac atttacctcc tgagtaagta ttcaagccca attcatactt aagtagcaag
      121 tagagattct ttatagggtc catcctatcc aaactattgt cttttttaaa cagagaagag
      181 gtaagattaa aaatttattt ctttattaca ttttctgatg aaaaaacttt ctctgcattc
      241 ctaaacaata ttttctcttt ttcttcatcc gtaattttag caaattttac tttatatatt
      301 gaaccagatg gttcatttat tgcattaccc gttcctaata gaatctggtt aggaatttct
      361 tttactaatt tttctattac tcctgcaact aatatatcag aaatttctat aaataggttt
      421 ttacattttt taaatgaagg aataagaaga ttgtttacct ctgaaaataa ttgagtgtca
      481 taaccacata taactatttt ctgtgaagag tattgttctg caaaatttat aatttctgaa
      541 ataggataaa taggggggga aaatcccatt ttgtttgcaa ttgagcagaa attataacaa
      601 acttcaactg ttatataaag aggtatattt tcttctttaa ataaatccat aaaggggtta
      661 aggaaaacaa tatcttttaa agaataatta tgaagagaag gagagattct tgcaatttta
      721 aaaggatatt tatcaaaata ttccattgtt tcttttaatc cccagatagg attaatacaa
      781 gcaacaggaa taaacctatt aagataggga aaaatttctt taatgagcca gagattacct
      841 tctttagtat cagttaccaa tgcaacagtt gaagtaacaa cagaaatatc aattttattt
      901 ttgtccataa ttttaagaaa ttcttctacc tttgttatcc cttttgtcct taatccccat
      961 cttccaagat aactatttga atcaataatc ataatttgag agtccttttt atatttaaac
     1021 ctaaaatttt ctttttgctt tcttctgaaa gtttagaatt tttaataatc cctaaattaa
     1081 ttgctggatt aacaagaaca aaatcacttc caaaaattac tctttcatct cctacattag
     1141 agaccgcctc ttcaagaaat ccttgctcaa aaatagttcc tgataggcag agtataatat
     1201 ttttttgttc tttagcaaac cgactcatct gtttccattt tgtatttcct atatgagcaa
     1261 aaataaaagt tgcttctgga tatttttgag aaataaattt agcttcttct atactatctt
     1321 cccctgtatg taagaaaaga gggatattta attctattgc tttctccact atgggaaaag
     1381 ttaaagggtc attataaggt atttgagaaa ggtcacagtg aagtttgata cctttcattc
     1441 cttctttaat acatttgtct aactcttcaa tagattcttt tccaaatagg ggatttaaag
     1501 tagaaaagcc aataatacgc gaaggatatt ttttcataag ttttaatacc ctgttatttc
     1561 ctaaagaaac atctgtcaga agataaaatg gacaacaaac cattgccata tcaataccaa
     1621 attggtctaa tgcagatata tattcttctt ctgaaattct aattcctttc atctcatagg
     1681 tattccagca agtatgagca tcaataatca ttttttaatt ctccattaat tttaatgttt
     1741 ggtttaaaat tatatcataa ctttcttctc ccagcattgc tactgaaact tcataaccac
     1801 cttctttata actttctttt gtaggtatat acaacaaatc gtcattcgta aggtaaagaa
     1861 tatatgtatc agaaaaaggt gcctttttct ttatttcaag tcctaattct acaaatgctt
     1921 ctcctgggat gccaataaaa accaaattat taacagaaaa agttataatt tctgaaataa
     1981 ttgttttttt atttctaaac cttctattca tatcccatcg cttaactatt tcctcacgaa
     2041 tgggaagttc aacaaaactt tttgctactt ttaattttac atctgaagta aatttaatat
     2101 tttttaatac tctttttacc tctattgcta aattttcccc taattctccc attctttgaa
     2161 atccttctaa agccctaata ggtccaatat tcccacacgc accatttgag aaaaaagcaa
     2221 cccctccaat gtcttcctct atcaatcttt ttgctactcc tggatagtca gcagaaatag
     2281 aaaggttatc tattggtaga catattgggt ggcatgtata attaaaaaaa gttgctattg
     2341 gttttgaatt catatcttca atacaaatta cacctacttc atcatctact ggcccttttg
     2401 catatttaag taattgaggt tcatcataaa gataatgatg acttccatct ggtaagttaa
     2461 ttctacgatt tacactctct ttaattgctc ctctccccat accaattttt gcttctttca
     2521 tatttctcaa tgcaatacaa ataccattag caattttctt gcaaagttgg taaatataag
     2581 aaatatcttt ttcaggataa ggaagaataa acctggtatt tggtccatag tgggtatgag
     2641 atgcagtaat taaaaaattc tctaaattaa ttttcccctt aacaagttct tttatttttt
     2701 ctgtaatttc tatatctatt cctattaaat ccgcagtaac tatagcgaat ttttcttttc
     2761 catcatcaag aatcaatata ttggcatata aattatcatg tatacttttt gctatatgtc
     2821 ctcttcccat acctgccatt tcacaaccaa ttgaaggtgt aatatctact ttggaaacac
     2881 cacatctaaa attttttccc atttattatc tcatctattt gtatctcctt agcaattttc
     2941 ctttcatctg cttttttcat aaaaacctcc ctaaaattat ttcatcaaca ccaacaaaaa
     3001 aatggtctct tctatatcct tctggaataa atccattttt tatataataa attaaagccc
     3061 ttttattatg tgcagatacg catgtggata ttttcctctg ttttttactt gcccaaaact
     3121 cttctgcttt tttcatcaaa atttttccaa cacccttact gaaatactct ggttcaactc
     3181 ccactatttc aagagaaccc aacccatttt ttcccgctgt tacacttata taaccaacaa
     3241 tctttttatt atcttctaca acaaaagaaa ttctctctga aaatgcatct ttaaaattag
     3301 attttttctc aaaaacagaa ggggaaaatt cattgaagtt ttctaattta tctcctaaaa
     3361 atgacttaaa tgcttttatc attaattcag aaacttgttt tgcatctccc tttttaaatt
     3421 ttctgcatct catttttctc atctcctctc ttaataaatc tgttaagatt ttttatattt
     3481 atatttttct taatttttta atatcttttt tatatcggaa aggatttctt ctctcgcttc
     3541 ctttatatat tctaaacttg cttcttttgc ggtttttgaa taccatttac tttcatcaaa
     3601 ttttttcata tcaactccat caggacaaaa ggtaagcatc aaagatgttt cttgtttgcc
     3661 tgcatggtca attggaaatt ttttttgtat ttcctctgta ataaaaggat gaatttttat
     3721 ccagttaaat ggatcctgcc ctttctcctg ttgtgtgtaa taatcttttg aactttcttt
     3781 tccccaccag ccctctccta aatttgcctc tataaactca aaaattgact gtcttgctgc
     3841 aaatttaaat gcgaggtctg ttggcatacc ttgaacaaaa ttttcactct gatgatgaat
     3901 aaaaatatgt atgtttctaa aaccaattct taataaccca ttaaagagcc ctttggcaaa
     3961 agggaaaagt gaaggacctg gtatatgaat tgttccattg ttttctggtg gttcaacacc
     4021 ataacttgct gctccataat aaaaaggtgg taaaattaca acatccatct ctttttcaat
     4081 aatttcaaga gctcttataa ttagtaaagt atcaacacca accactaaat gctcactatg
     4141 atattcaatg actccaacag gtaaaacaac tggggtattg tcttttattg cttttcttat
     4201 ctggtccgga aacatcattt catacttcat ttctccccct ctttattttc aattttaata
     4261 ttttaaaatc cctctcatac cccattgtgc ttggaaaatt ttcctatttt ttgacattac
     4321 acttcaatta ttttcatcag ttcactactt aataattcag caccttcttt ttttactatc
     4381 acaccgtttt cccaccttaa accaaagtca tcacaatata aaaatgtatc aacttgaaat
     4441 gtcatatttt cttcaagata ataatcagag tttgtttcca tccagggtct ttcaacttca
     4501 atcatcccaa ttccatgaca cgggccataa aggatatttt tcccaactcc tattttttct
     4561 ccatattcaa aaaatttctt agcaacatcc cctgctttaa ctccgctttt aatccagtca
     4621 attgtttttt tgtgcatatc aagcccagca gagacaagtt ttttcatctc ttctgacatt
     4681 ttccctatac atacaggtct tcctacactt gaagaatatc ctgctactct tgccccaata
     4741 tttaactgaa ttaaattgtt ttttcctaat tttgtatacg aaggtctacc aattgcattt
     4801 gttgagtttt ttccggcaag aacatactga ggatgtcctt catattctgc tccattttta
     4861 tataaggact cctcaataat tccaacaagt tgtaattctg tcatttctgg tttcatattc
     4921 tcaattgctt cttttaatgc aatttctgaa attttaaatg cctctttcat catttttatt
     4981 tcaacttcac ttttttttat tctcatatca accattattt catcactttt tattatttct
     5041 gcatcaggtt ctgcttgttt tattgcttca tagataggta taggaaatat ttggtatcca
     5101 gcaattccga ttttctttac tttttctcca accatctctt tgaaaacaat tgaaaaatta
     5161 tcaagtttca tccctggata ttctggttct gcactttctc tatactgtaa aattctatat
     5221 atctttttaa ctctgcttct attcattgca aatgtttcac tttcaggtcc aataagaagt
     5281 gcaattcccc catttttaga aattgcaacc ccagatgtct caaatatagg ccagtaatca
     5341 gtcaaatacc ttacatttgc aaaatctgct tcatttgatt gaacaatcaa aatatccagg
     5401 ttttttgctt tcactttctc aactacttcc ttccatcttt tttcaaattc actatccggt
     5461 atttttattt ccatttttta ctcctttttc atatatacca catgattttc ttataattaa
     5521 ttttgtatca attttcactt ttttaatatc actgaatttt cttttccctt ttattttatc
     5581 catcaataat tctgctgcac tctcccctat tttctctgct tgctggtcaa ctgtcgttaa
     5641 aggggattct aatatctgac cgacttccat atttccataa cccactaaag aaatatcttc
     5701 aggaactctt aaatttaatt ctctaagtgc tttgtatgca cctactgcaa cctcatcatt
     5761 acaggcaaaa attgcatctg cattatagtt taaaaaatat tttttagttt caaaatatcc
     5821 atcttctaaa ctccactttg taaatcttat tattttctct tttattttat atttttcaat
     5881 tgcatttata tatccatttc ttctctcatc tgcacttgaa tcaccaacag gtcctaataa
     5941 atgtaaaatt tttttattcc cgagttctat taaatgttct gttattaaaa atcccccttt
     6001 atagtcatct gacataacta tatctgcaga aataccagga atttctacat ctattattac
     6061 aataggtatc tctttactaa ttttgtcaac tgttgacctg tttgtatgtg tataataaga
     6121 ggaaataaga aataacccct tccttttttc ttttaaaatt tttcttaaat atatcttttc
     6181 ttcaattggg ttatctcttt ccgaaaaaat tgtaaattct attccttctc tgcttaatac
     6241 tttctggatt cctgaaatta tctggaaata aaaagagttt cttaaatttg gcacttttat
     6301 tacaatcagg tcttttattg tattttgccc attattaaat tcctgtgtta aaaaagtccc
     6361 cttacctggc attctaaaaa gtaagttctc atttatcaat tcaattattg cttctcttac
     6421 agtcattctg ctaataccaa attcctctgc taatttttct tctgaaggaa ttttttcccc
     6481 tggaagaatt ctcccttttc ttatatcttc aagaagtttc tctttaattt gttttgataa
     6541 cggtacataa ctattacttt ttatttttcc cattttttat tttcctcatt tttttgtctg
     6601 atatcattat acattgaaaa aaaattttgt caagaggtaa aattgaacct aaaattctgt
     6661 tatgggaaga tttttaaaga taccatcctt ttctctgcaa actataaaaa tcatatggta
     6721 taatgccaag aaaaaatatt atttttataa tagatatgtg aaaaattatt ttatttttgt
     6781 ataaaaccat cctgttctaa atattttaaa acctctctca caggtgttct gcttaccttg
     6841 aattttttac aaaggatttc ttctgttaaa tgactattat tatttaaaat aaattcgtta
     6901 atcttttccc ttgatttatc tttttttgtt tccattttat tccccttttt gtattcaatt
     6961 tatcatcctt atttttttgt taagtcattt tctaatttgc tattccttct gtttttatat
     7021 attttcacat cttttttgta tacattataa tctattcttt tcaagataat tttcaacaaa
     7081 gttaatttat aaatttttct cttcatgtaa tttcaatatg aaaccatcta attattgcca
     7141 cgcattcttc aacacaccaa aatgcagcaa aaatttcttt attattcagt ttaataatag
     7201 ttggtaaacc aaattttaaa atattcattt ctttcactat attcctttgc ttacgagtaa
     7261 gttgggaaaa atgttcgccc cataaaggta cttcttcatc gttaatccac ttatttccat
     7321 ctaaatgtga aatattagcc cataaacctg gtttatcagt tcttctgtat aaactcaaaa
     7381 ttttattatc ttcaagatat ataggagtac atgtctgtcc tttcaaacct gttgattgag
     7441 gggcagaaaa agtttttgcc ctatcctgag aaatagcata atggatttcc atgtctttct
     7501 tgtcttttag attatgtgtc cagcaaacag acagaagttg ttcattcgca atttttatta
     7561 gtttttgttc ccaataaaca atttctttgt tagaaccatc cataacaata gaatattcag
     7621 accatgtttt tcctttatca taagaacgca gaactattgc tttcattcct attgggtctt
     7681 ccccattcca tcccttccat gttgatgtcg gaattaacca ttcatttgaa gttaatgcaa
     7741 caacaggtga acagatttca aaaggatgtc ctttaattgg tggaataatt atttctggtt
     7801 ttgtccatat aaatccttta tcctttgaac gaattaaaaa aagttcagtc ggaacaaatc
     7861 catttgtttc tggattgaga agttcttgct catcactatt gcgtataaaa tatgcaccaa
     7921 acccaataat ttcaccatca gcacataaac ttatacgaca tgtagatgat ataaaatctc
     7981 ttttgataac aggcaaatct ccttcaaaga cccatgtttt tccaccatca gaagaacggg
     8041 aatatacagg atggcaatca acactatcaa aagcactacc tatggtaaaa gatacaagaa
     8101 tatctccatc gcgtaatagt acaagagaag ggaaatacgc atgcaaactt ttcaaatgtg
     8161 gttttgaatt ccgatagaca atacccgatt ttttaacttt tatcattctt tttcttctaa
     8221 aaagtatttc taaaaatatt taatacttta ttatttttat atatcatttt tcttttcctg
     8281 tcaacttctt cacttcttta aatgaaatgt attatcttct atattacaaa aagccaagat
     8341 ataattatat tttttgaatg ttttaacttt aatttcttta ttttcaaatt caggaaataa
     8401 agataaaaat tttcctttta attttacttt gaatttttct ccatctgtgc tttgaataaa
     8461 aatatctttt ggagagaaaa caaaaccagt ccccaatgct tttgcaactg cttcctttac
     8521 acaccaaaga gtaattaatg aaataccttt tgttttctca atttcttctt ttgataaaaa
     8581 ttttttagca atttttttta tatttttatt cttcttttca atatctattc caataaactt
     8641 agaatcccca caggtagcaa cacttatccc atctgtatga gaaattgaaa gataaatttt
     8701 tttgtttttc aaaaaacaat atgcgttccc cttttgctct ctgtttattt ttatttcttt
     8761 atatcttaat ttttctctgc caaaatattg agaaacacca aattttccaa caattctacc
     8821 ggagataaat tgtattttcc tttttctgtt tttaaaattt ttaaattcct ctttctcttc
     8881 tctggtgaaa aaaataaaaa gaaaaaaagg taaaaaaaga aagaaaaaac aatcaatttt
     8941 tataaaataa caattatcaa tttctttaat tttttttatc attttcttta tcaatattaa
     9001 gttattataa ctttaaacct gacaataaaa aaattaaaaa atgcagttcc attcaaaaat
     9061 ttgacaaaaa aataaatatg ttttatactt ttcatagtta aaattttatg atggttgttt
     9121 cttgtttttc agagttggta cgtcatttcc ccctttaaca aaggcctgtc cgacgcgctt
     9181 tttggcggag ggata
//
LOCUS       DAOOVW010000060         8977 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_4827_length_8977_cov_9.061533, whole genome shotgun sequence.
ACCESSION   DAOOVW010000060 DAOOVW010000000
VERSION     DAOOVW010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8977
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4827_length_8977_cov_9.061533"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..434)
                     /locus_tag="PLW95_07315"
     CDS             complement(105..434)
                     /locus_tag="PLW95_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOV22462.1"
                     /translation="MKELLNNLKLTINELSKKNPEGLKKFNEFMECVLKNGSLDTKTK
                     ELIAIGIAVALRCPYCIGGHTEKALKAGATEDEIYEAGMVAVLMAGGPALTYITELKK
                     AIELFKK"
     gene            complement(447..920)
                     /locus_tag="PLW95_07320"
     CDS             complement(447..920)
                     /locus_tag="PLW95_07320"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOV22463.1"
                     /translation="MAIFFDVSEIYQFAINIEENGEKFYRKVSSKLREPKIKEIFSFL
                     ADEEGEHKKTFEKLLEDIKNYEPVETYPGEYFAYLKAYSQELIFPQMIERELDKSITP
                     IDAINFGIRRELESILYYIEVKNFVQETQHSQLDKIIEQERQHFIKLSNLKKEMK"
     gene            complement(933..2114)
                     /locus_tag="PLW95_07325"
     CDS             complement(933..2114)
                     /locus_tag="PLW95_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOV22464.1"
                     /translation="MPIKEIKKGIWAIMINDWDRRIFDELIPLPEGTSYNAYFLKDDK
                     NVLIDTADPRKKEELFEALEKINVKKIDYIVSNHSEQDHSGCLPTIIEKFPEAKIITS
                     EKGKEFLSSLLLIPENNFIVMKDCEKLSIGEKKLEFISAPYVHWPETILTYEEKNKVL
                     FPCDLFGSHLATSEIYASNNINVLTSAKRYYAEIMMPFRTFIKGHIKKLENYEISLIA
                     PSHGPIYNNPSLIINAYNDWVSDNVKNEVIVIYVSMHGSVKKMIDYFVDRLIEKSIVV
                     KPFNLTETDIGQLAISIVDAATVVIGSPTVLTGPHPVVLYASYLFKILKPKTKFLSII
                     GSFGWGTNMIKLLSEMIPSKDIDVLEPVMIKGYPKENDFALIDKLAEEIYKKHKEIKI
                     I"
     gene            complement(2114..2275)
                     /locus_tag="PLW95_07330"
     CDS             complement(2114..2275)
                     /locus_tag="PLW95_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOV22465.1"
                     /translation="MERYVCQVCGYVYDPEKGDAENGIKPGIPFETLPDNWVCPVCGA
                     SKDMFNKEE"
     gene            complement(2323..2754)
                     /locus_tag="PLW95_07335"
     CDS             complement(2323..2754)
                     /locus_tag="PLW95_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008694928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOV22466.1"
                     /translation="MNEKDIRKLFEEYGIQPSFHRFKILQFLMENKNHPTVEQIYRHL
                     VKDIPTLSKTTVYNTLRYFVSKGIVAEIMIEENEVRYDFETTPHIHFKCKKCKNLYDI
                     FLDCDILKKQQIDGHKIEEHHIYLLGICKNCRTIDNNKKQK"
     gene            complement(2751..3365)
                     /locus_tag="PLW95_07340"
     CDS             complement(2751..3365)
                     /locus_tag="PLW95_07340"
                     /inference="COORDINATES: protein motif:HMM:NF002305.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosylase/DNA lyase"
                     /protein_id="HOV22467.1"
                     /translation="MDVMITNLQRIYDDIKTIIKEKLECFNNLNKKEIKMEFIFCLLT
                     PQSEAKKCWQVVLELKEHGFPLAEEKIKNCISGVRFKNKKANYIKEGISKFESLYSKI
                     KEEKDVFKLRKDIVMKIKGIGWKETSHFLRNIGKGENFAILDRHILKCLCKYGVIEKI
                     PESISQKQYINIENKMREFSKNIKIPLSHLDFLFWYIEKGEIFK"
     gene            3492..4457
                     /locus_tag="PLW95_07345"
     CDS             3492..4457
                     /locus_tag="PLW95_07345"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOV22468.1"
                     /translation="MLFILIILSSLYMGWSMGANDAANCVGADIGSGVMKLRDGIIIT
                     CVFSFLGSILLGHKVIKTIGKGVVPLNLLPPLQSDLISLAAILGAAIWVTIATYKKYP
                     VSTSHSIVGAVAGGGLAFKTVIQWMKIKQIFICWVLTPFGSAIITILLYPLIKFIFSF
                     SAIKKYEKGLILFSIYVTSAYLAFSWGANDVANATGILIGTGNITPNGAAIIGALAIT
                     LGITTWGYKVIETIGFNIVQLTPLMTIAVEVSSAINIHLYTHYGIPVSTSHSIVGAIW
                     GIGILQGIKTINWKIAKDIMLTWALTPVISGLITFIFLRIINAFL"
     gene            4471..5139
                     /locus_tag="PLW95_07350"
     CDS             4471..5139
                     /locus_tag="PLW95_07350"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOV22469.1"
                     /translation="MERSRNIFVWLGQREKKEILNAELQHMELSFECVKEMKNCFQAY
                     YENKTEEKNNAISKVKEYEKKGDEIRKTITLELSKGYLMPADREDLLLLNTKLNDIAD
                     SAKGVARLFEFIEGIKMPELSKSLLDNSIIAVKSIEKLKNAIVSLIKDEIDRVLEECS
                     QIEILEEEGDDKRRELLGQLLKSEISLPISIIFYEIIDTTENLIDTVKNTADLVRVLA
                     IKLK"
     gene            complement(5144..6430)
                     /locus_tag="PLW95_07355"
     CDS             complement(5144..6430)
                     /locus_tag="PLW95_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine-DL-glutamate epimerase"
                     /protein_id="HOV22470.1"
                     /translation="MKIKKVNLNFEYEPFKINLGFKGNYVTGLWEVITYLESENNYGI
                     GLGTQSVLWSDADVFISNSAAGGNALMFSTTQFALNLLEGKNIESPVETIEEIFDDVH
                     EYAKKITGKKNLRKTFTLNSLVSVDNALWSLYAKETGNNDFFNMIPAKYQSTLKYRHE
                     KLACIPLISYSVSIDEVEKMVKEGYFVLKIKIGSDPEGDGDRDKMLKWDMERIKNIHH
                     QLKNYTTPYTKSGKLPYYFDANGRYDTKERLWELIDFMDKIGCLSQVVIVEEPFSEDN
                     EIDVSDFPVRIVADESAHTDKEIIKRCQMGYKGVALKPIAKTLSMTLKILNECEKRNM
                     DYFCADLTVIPPLIDWNKNLSARIKPLTEMKIQMVEANGFQHYENWKKLLSYHPFYGK
                     KWVDAENGIFVLDNDFYEKSGGIFDCSPHYKKLVIS"
     gene            6802..7482
                     /locus_tag="PLW95_07360"
     CDS             6802..7482
                     /locus_tag="PLW95_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOV22471.1"
                     /translation="MEIKSYSLGISFVAGVLSFLSPCILPLIPIYISYITGISIEQLQ
                     EPKNTLKIFIISLFFISGFTLIFVLMGASATAIGNFILKKKNILRIIGGIIIIIFGLH
                     LTGILKIKKLYTEKRIVLKNKKTGYLSSFLLGMAFSAGWTPCVGPVLSSILIIAANEK
                     TVFRGIILLFTYSIGIGIPFLITSLLLNKLLNFFNKIKKHYRVIEIIMGFLLVILGVL
                     FLLNKFIF"
     gene            complement(7472..8686)
                     /locus_tag="PLW95_07365"
     CDS             complement(7472..8686)
                     /locus_tag="PLW95_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007199368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOV22472.1"
                     /translation="MNKIKGNMLIAQSGGPTVVINQSLVAAVLEAKKHKEIKGIYGAL
                     YGVKGVLEENLIDLKKESNSNLKKVAFTPCAALGSVRKKPTDEECEKIFKIMTKYDIR
                     YFFYIGGNDSAETTYILNESAKKVGYEFRCFHIPKTIDNDLKENDHTPGYGSAAKFVA
                     MAVMGDNLDNKSLPGVKIDVVMGRHAGFLVASSSLAKIYEDDGPHLIYFPERPFIMEK
                     FLSDVDNVYKKYGRCLITVSEGISDEKGEPIASKFTKEVDAHGNVQLSGTGALGDLLA
                     DIVKNKLNIGRVRADTFGYLQRSFPGIYSEIDAKEAYKVGEIAVKSAISGDFGSSGSV
                     SIKRKKSKKYSIYYEIVPLSNVAKETRSMPPAFINSENNFVTSAFIEYAKPIVGKLPE
                     IGILKKIKVPKR"
BASE COUNT         2974 a         1437 c         1121 g         3445 t
ORIGIN      
        1 ccattcatcc gaaagtgcct tattaagcaa ttttactaat tcttctacat ctacactaat
       61 tatttcttta cctattcttt ccataatttc ctccttttta atggttattt tttaaatagt
      121 tcaattgctt tttttaactc agttatatat gtaagagcag gacctcctgc cataagaaca
      181 gccaccattc ctgcttcata aatttcatct tctgtcgctc ctgcttttaa tgctttttca
      241 gtgtgcccac ctatacaata aggacatctt aaagcaactg ctattccaat agcaattaat
      301 tcttttgttt ttgtatcaag ggaaccattt tttaagacac attccataaa ttcattaaat
      361 ttttttaatc cttctggatt ttttttagaa agttcattta tcgttaattt aagattattc
      421 agtaattctt tcactctatc acctccttat ttcatttcct tttttaaatt tgagagtttt
      481 ataaaatgtt gtctctcctg ttcaattatt ttatctaatt gggagtgctg tgtttcttga
      541 acaaaatttt taacttctat ataataaaga attgattcta attctcttct aatgccaaaa
      601 tttatcgcat caataggagt tatactttta tcaagttctc tttcaatcat ctgaggaaaa
      661 attaattctt gagaatatgc ctttaaatat gcaaaatact ctccgggata tgtttctact
      721 ggctcgtaat tttttatgtc ttcaagcaat ttctcaaatg tttttttatg ttcaccttct
      781 tcatcagcca aaaatgaaaa aatttcttta atttttggtt cccttaattt agaagatact
      841 tttctataaa atttttctcc attttcttca atatttattg caaactgata aatctcactt
      901 acatcaaaaa aaatcgccat tatccccctc ctttaaatta tctttatctc tttgtgtttt
      961 ttataaattt cttctgcaag tttatcaatc aaagcaaagt cattttcctt tggatagccc
     1021 ttaatcatta ctggctctaa aacatcaata tcttttgatg gaatcatttc tgacaataat
     1081 tttatcatat ttgttcccca tccaaaagaa ccaattattg ataaaaattt tgttttaggt
     1141 tttaaaattt taaacaaata ggaagcataa agaacaactg gatgtggtcc tgtaagaact
     1201 gttggagaac caataacaac agttgccgca tcaacaatcg aaattgctaa ctgcccaata
     1261 tctgtttctg ttaaattaaa tggtttaacg acaatgcttt tttctattaa tctatcaaca
     1321 aaataatcaa tcattttttt aacacttcca tgcattgaaa cataaataac aataacttca
     1381 tttttaacat tatctgaaac ccaatcatta taagcattta taataagaga tggattatta
     1441 tatattggtc catgacttgg agcaatgaga gaaatttcat agttttcaag tttctttata
     1501 tgtcctttta taaatgtcct gaaaggcatc ataatttctg catagtatct ttttgcagaa
     1561 gttaaaacat ttatattatt acttgcgtaa atttcgcttg ttgctaaatg agaaccaaaa
     1621 aggtcacatg ggaatagtac tttgtttttt tcttcatatg ttaaaattgt ttcaggccag
     1681 tgaacatatg gtgcagaaat aaattccaat ttcttctctc caatggataa tttttcacaa
     1741 tctttcataa caataaagtt attttcagga attaaaagga gagagctcaa aaattctttt
     1801 cctttttcac ttgttataat ttttgcctcc ggaaattttt caataattgt aggtagacac
     1861 cctgaatggt cctgttcaga atggtttgaa acaatataat caatcttttt aacatttatt
     1921 ttttcaagtg cttcaaacaa ttcttctttt tttctcgggt ctgctgtatc aattaaaaca
     1981 tttttatcat cttttaaaaa ataagcatta taagatgttc cttctggtaa aggtattaac
     2041 tcatcaaaaa ttcgtctatc ccaatcattt atcataatag cccagatacc cttttttatt
     2101 tcttttatag gcattactcc tccttgttaa acatatcttt acttgctcca caaacaggac
     2161 aaacccaatt atcaggtaat gtctcaaaag gaattcccgg tttaattcca ttttcagcat
     2221 ctcctttttc cgggtcatat acatatccac aaacctgaca tacatatctt tccattcttc
     2281 cctcctcttt tgtattgatt tcaactttgc tattatttca ttttactttt gttttttatt
     2341 gttgtcaata gtccggcaat ttttacatat acccaataaa taaatatgat gttcttcaat
     2401 tttatgtcca tctatttgct gctttttcaa tatatcacaa tccagaaaaa tatcataaag
     2461 atttttacat tttttacatt taaaatgaat atgtggagtt gtttcaaaat catatctaac
     2521 ctcattttct tctatcataa tttcggcaac tatccctttt gaaacaaaat atcttaaagt
     2581 attataaaca gttgtttttg aaagagttgg tatatctttt acaaggtgtc tatatatttg
     2641 ttcaacagta ggatgatttt tattttccat aagaaattgc aaaattttaa acctgtgaaa
     2701 agatggctga attccatatt cttcaaataa ttttcttata tctttttcat tcatttaaat
     2761 atctctcctt tttcaatata ccagaaaaga aaatcaagat gagataatgg aatttttata
     2821 tttttagaaa attccctcat tttattttca atatttatat attgtttttg tgaaatactt
     2881 tctggtattt tttctataac cccgtattta caaagacatt tcaaaatatg cctatcaaga
     2941 attgcaaagt tttcaccctt cccaatattt ctcaaaaaat gacttgtctc tttccatcca
     3001 atacctttaa ttttcataac aatgtccttt cttaatttaa aaacatcttt ttcttctttt
     3061 attttagaat ataaactttc aaatttagaa ataccttctt taatataatt tgctttttta
     3121 tttttaaacc tgacacctga aatacaattt tttatcttct cttcagctaa tggaaagcca
     3181 tgttctttta gttctaaaac cacctgccag catttctttg cctctgattg aggtgttaaa
     3241 aggcagaaaa taaattccat ttttatttct tttttattta aattattaaa acactcaagt
     3301 ttttctttaa taattgtttt gatatcatca tatattcttt gcaagtttgt aatcattaca
     3361 tccatataat aagtataata atttttttat ttttcgtcaa taaaatgtat aaaaaaataa
     3421 tgtttgtccc tcttatataa tttttgtttt aaatagataa gtatattata attaaggaaa
     3481 agggcatttt aatgttattt atacttatta ttttgtcttc tctttatatg ggttggtcaa
     3541 tgggagcaaa tgatgctgca aattgtgtgg gtgcagacat tggctctggt gttatgaaat
     3601 taagagatgg aattataata acatgtgtct tctctttttt aggttcaata ctgcttggac
     3661 ataaggtaat aaaaacaata ggaaaaggag ttgttccatt aaatctactg cctcctttgc
     3721 aatcagattt aatttctctc gctgctattt taggagcagc aatatgggta acaattgcaa
     3781 cttataagaa atatcctgtt tctacatctc attctattgt tggtgctgtt gcaggtggag
     3841 gacttgcttt taagacggtt atacaatgga tgaaaataaa acaaattttt atatgctggg
     3901 tattaactcc ttttggttcc gcaattataa caattttgct ttatccttta ataaaattta
     3961 tattttcttt ttctgctata aaaaagtatg aaaaagggct aatattattt tctatatatg
     4021 tcacaagtgc ttatcttgct ttttcatggg gtgcaaatga tgttgcaaat gcaacaggta
     4081 ttttaatagg aacaggtaat attactccaa acggtgctgc aataattggt gctttggcaa
     4141 taactcttgg aattacaaca tggggatata aagtaattga aacaattggt tttaatatag
     4201 ttcaattaac tcctcttatg acaattgcag ttgaagtatc ttctgcaatt aacatacatc
     4261 tctatactca ttatggaata cctgtttcta catctcactc aatagttggg gcaatatggg
     4321 gaatagggat acttcaaggt ataaagacaa taaactggaa aattgcaaaa gatataatgc
     4381 ttacatgggc attaactcct gtgatttctg gtttaataac ttttattttt ttaagaataa
     4441 ttaacgcttt cttataacag gaggtgcaaa atggaaagaa gtagaaatat atttgtatgg
     4501 ttaggacaaa gagaaaaaaa agaaatactt aatgctgaat tacaacatat ggaattatcg
     4561 tttgaatgtg tgaaagagat gaaaaattgt tttcaggcat attatgaaaa taaaacagaa
     4621 gaaaaaaata atgcaataag taaggtaaaa gaatatgaaa aaaaagggga tgaaataaga
     4681 aaaacaataa ctcttgaact ctcaaaagga taccttatgc cagccgatag agaggattta
     4741 ttgcttctta atactaaact taatgatatt gctgatagtg caaaaggtgt tgcaaggtta
     4801 tttgaattta ttgaaggaat aaaaatgcca gaactttcaa aaagtttact tgataatagt
     4861 atcatagcgg taaaaagtat tgaaaaactt aaaaatgcaa ttgtttcttt aataaaagat
     4921 gaaattgaca gagttttaga agaatgttct cagatagaaa tacttgaaga agagggagat
     4981 gataaaagga gagaattact tgggcaactt ttaaaaagtg aaatatcttt accaatttca
     5041 attatttttt atgaaataat tgatacaact gaaaatctta tagatactgt aaaaaatact
     5101 gccgaccttg ttagggttct tgctattaaa ttaaagtaaa tggtcaggaa ataacaagtt
     5161 ttttatagtg aggagagcaa tcaaaaattc ctccactttt ttcataaaaa tcattgtcaa
     5221 gaacaaaaat accattttct gcgtcaaccc actttttccc ataaaaagga tgataagaaa
     5281 gaagtttttt ccagttttca taatgctgaa aaccatttgc ttctaccatt tgaattttca
     5341 tttctgtcaa tggttttatt cttgcagaga gatttttatt ccaatcaatt agtgggggaa
     5401 taactgtaag gtctgcacaa aaatagtcca tatttctttt ctcacactcg tttagaattt
     5461 tcaaagtcat acttaatgtt ttagcaattg gttttaaagc aactccttta taacccatct
     5521 ggcatctttt tataatttct ttgtctgtat gagcactttc atctgcaact atccgaacag
     5581 gaaaatcact tacatcaatt tcgttatctt ctgaaaatgg ttcttcaact ataactacct
     5641 gtgatagaca acctatttta tccataaaat caatcaattc ccatagtcgt tcttttgtat
     5701 catatcttcc attagcatca aaataatatg gtaatttacc tgattttgta tatggtgttg
     5761 tataattttt aagttgatgg tgaatatttt ttattctttc catatcccat ttaagcattt
     5821 tatctctatc accatcacct tctgggtcag aaccaatttt tattttaaga acaaaatatc
     5881 cttcttttac catcttttct acctcatcaa ttgacactga atatgaaatt aaaggaatac
     5941 aggcaagttt ttcatgtcta tatttcaaag tagattgata ttttgcagga atcatattaa
     6001 aaaagtcatt atttcctgtt tcttttgcat aaagagacca taatgcatta tcaacactaa
     6061 caagagaatt taatgtaaat gtttttctta aatttttttt acctgttatt ttttttgcat
     6121 attcatgaac atcatcaaat atttcttcta ttgtctctac gggactttca atatttttcc
     6181 cttctaataa atttaaagca aactgagtag ttgaaaacat cagtgcattt ccacctgctg
     6241 cactattact aataaaaaca tcggcatctg accagagaac actttgagtt ccaagtccaa
     6301 ttccataatt attttctgat tcaagataag ttattacttc ccataatcca gttacataat
     6361 ttcctttaaa acccaagttt atcttaaatg gttcatactc aaaattcaaa ttcacttttt
     6421 taatcttcat ttcatccccc tttacccaat taggtactgc caaaattggt taattaaatt
     6481 cactcccctt tcataaaggg gagagtgttt ttcccttcct ttataaaagg aaggagttaa
     6541 aatttcccct ttgacagcgg agtgagaggg atttttattt cccatctttc tttcatctaa
     6601 gtttttgaca ctattcaatt atacttcaaa acaataaagt acattattat ataaataaca
     6661 taaatacata aaaagtaaaa atttttttgg ctcaaacttt taaatggtat aattttattg
     6721 taaatactca gttccaaaaa aactgaaagc agagagtatt attgaaaagc acaaagtaaa
     6781 aaatataaat tacaaaatat aatggaaatt aaaagttatt ctcttggaat aagttttgtt
     6841 gctggggttc tttctttttt aagtccctgt attcttccac ttatcccaat ttacatttct
     6901 tatataacag gaataagtat tgaacaatta caagaaccaa aaaatactct aaaaattttt
     6961 attatttctt tgttttttat atctggcttt actctaattt ttgttcttat gggtgcatct
     7021 gcaactgcaa ttggaaattt tattttaaag aaaaaaaata tattgagaat tataggtggt
     7081 atcataataa taatttttgg ccttcactta actggaatac taaaaataaa aaaattatat
     7141 acagagaaaa gaattgtctt aaaaaacaaa aaaacaggat atttaagttc tttcctgtta
     7201 gggatggcat tttctgctgg atggactccc tgtgttggcc ctgttctttc ttcaattcta
     7261 ataattgctg caaatgaaaa aacagttttt cggggaataa ttttgttatt tacctattca
     7321 ataggaattg gaataccttt tcttataaca tctctattat taaataaact tctaaacttt
     7381 tttaacaaaa taaaaaaaca ttacagagta atagagataa ttatgggttt tctccttgtt
     7441 attcttggtg tcttatttct tctaaataaa tttatctttt agggactttt atttttttta
     7501 gtattccaat ttcaggtagt ttcccaacaa ttggtttagc atattcaata aatgcagaag
     7561 taacaaaatt attttcactg tttataaagg cagggggcat acttctcgtc tcttttgcaa
     7621 catttgatag tggaacaatt tcataataaa tactatattt ttttgatttt ttccttttaa
     7681 ttgagacaga accagaactg ccaaagtcac ctgaaatagc actttttact gcaatttcac
     7741 caactttgta tgcctctttt gcatctattt cagaataaat tccaggaaaa gacctctgta
     7801 aatatccaaa agtatccgct ctcactcttc ctatatttaa tttgtttttt acaatatctg
     7861 ctaataaatc accaagagca ccagttccac ttaattgaac atttccatgt gcatcaactt
     7921 cctttgtaaa tttagaggca attggctctc ctttttcatc ggaaattcct tccgagacag
     7981 taataagaca tcttccatat tttttatata cattatcaac atcagaaaga aatttttcca
     8041 taataaaagg tctttcagga aagtatataa ggtgtggtcc atcatcttca taaatttttg
     8101 caagagaaga agatgcaacc aaaaaaccag catgcctgcc cataacaaca tctattttaa
     8161 ctcctggcaa tgacttatta tctaaattat ctcccataac agccatagca acaaattttg
     8221 cagcagaacc atatccaggt gtatggtcat tttctttcag gtcattatca attgtttttg
     8281 gtatatgaaa acatctgaac tcatatccaa ctttttttgc actttcattc aaaatataag
     8341 ttgtttcagc agaatcattt cccccaatat aaaaaaagta tcttatatcg tattttgtca
     8401 taattttaaa tatcttttca cattcctcat cagttggttt tttcctcaca cttcccaaag
     8461 cagcacacgg agtaaaggca acttttttaa gattagaatt gctttctttc tttaaatcaa
     8521 ttaaattttc ttcaagaacc ccttttacac catataaagc accataaatt ccttttattt
     8581 ctttgtgttt ttttgcctca agaactgctg caactaaact ttgatttatt acaacagtag
     8641 gtcctccact ttgagcaatt aacatattac cttttatttt attcatatcc acctccattt
     8701 tctctataat tttgttatag aaattatttt aacatatttt ttttcatttg ggaaatgaat
     8761 ttcactactt aattcttgca gagttagaag ttaatttgca tgtctgaaag atacaaaaac
     8821 ataaatggaa agtcaaagaa aatgaatttt ttatgaaagg agtaaatttt tatagattct
     8881 gattactaat atttaaaaat tttactataa atagtattta aaattttgaa agagaaataa
     8941 gttgatttaa aaatttttta tttatattgc ctttcat
//
LOCUS       DAOOVW010000061         8797 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_4937_length_8797_cov_10.488561, whole genome shotgun sequence.
ACCESSION   DAOOVW010000061 DAOOVW010000000
VERSION     DAOOVW010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8797
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4937_length_8797_cov_10.488561"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(595..3366)
                     /gene="uvrA"
                     /locus_tag="PLW95_07370"
     CDS             complement(595..3366)
                     /gene="uvrA"
                     /locus_tag="PLW95_07370"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOV22473.1"
                     /translation="MEPKFISIRGAREHNLKNINIDIPRNNLVVITGVSGSGKSSLAY
                     DTLYAEGQRRYIESLSTYARQFLEQMKKPDVDHIFGLPPAIAIEQRKAASNPRSIVAT
                     TTEIYDYMRLLFARIGIPHCPACGKIISRQSSSEIIDNILSFPPTKEVKILAPVVRGR
                     KGEYVKVFDEIKKSGFLMVRVDGKYYEVDEKIKLEKYKIHNIEVLIDQFIIGEEERSR
                     VAESVEMGLKVGKGVIIVDDGQKEVFFNENFGCVKCGISFEELSPRMFSFNSPYGTCP
                     ECKGLGFLMRIDPDLVIPDKNKTFRKGAIRPWHETGGRGIFLYYRSLLRELLYQFNKG
                     LDDSPSDLTEEQLNILLYGSDEYNFEGVIPNLERLFKQTESERRKEEIMKYIREIKCP
                     VCNGGRLRKEALSVTVGGKSIMDITSMSVKGVKEFFNTLKLNEREKMIAKQILKEIIS
                     RLNFMEEVGLDYLTLDRMTHTLSGGEVERIRLATQIGSGLVGVAYILDEPTIGLHQRD
                     NLRLINTLKSLRDLGNTVVVIEHDEEMMKNADWVVDLGPGAGIHGGYVVAEGKVDDII
                     KVKNSITGKFLSGEREITLPSKRRKPTGKRIKIIGAKHNNLKNINVEIPLGLFVCVTG
                     VSGSGKSSLIEDVLYNALRKILYKSKEEPGEHDAILGIEYINKVVEIDQSPIGRTPRS
                     NPATYTGAFTYIREIFANTEEARIRGYKPGRFSFNVKGGRCEACIGEGITKIEMHFLP
                     DIYIPCEVCKGTRYNKETLEIKYKGKNIAEILDMKVEESVEFFKNFPHLKNKLQTLYD
                     VGLGYISLGQPATTLSGGEAQRIKLAAELSRKGNERTIYILDEPTVGLHLADIEKLLG
                     VLNRLVEKGSSVIVIEHNLEVIKYADWLIDLGPEGGDKGGYVIAQGPPEKIAKNPKSY
                     TGKFLKEKL"
     gene            complement(3826..4512)
                     /locus_tag="PLW95_07375"
     CDS             complement(3826..4512)
                     /locus_tag="PLW95_07375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOV22474.1"
                     /translation="MKKSKVGNGFTLIEILISMFILVIIFGLITVLFARASTVRKIVV
                     AHSEIQQTLSQMMDTIVHGKRGGWGLADASCIRSITLNEGTYDDTCVSYNTLVAYNET
                     QNETMIVRIASERELTDEGDNTKTLWVGWYSDPLPPDASNFYNEEALIDINNKVELVV
                     NNDEPENTRYSNFTYYDSRGRDIITGELDTETTLVKIVLSAKSKDPGLKNKAPVVLQT
                     AIKLKNALPF"
     gene            complement(4644..5153)
                     /locus_tag="PLW95_07380"
     CDS             complement(4644..5153)
                     /locus_tag="PLW95_07380"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOV22475.1"
                     /translation="MKNYKNGFTLLEMVISIFLIAIVIGIVLLLMSSNMNVVNKANEI
                     MIANALSQYSIEEVKNFEFPPVYSDRWGEQKPEEYGKIVDEIEDENSINIGTGTDFTP
                     PEFQDRFKIERYVIGYGNDTSTPLSNWATSPDYDKAMGLRVIVYVLRRKGNAVLSRRE
                     IYISRNGLF"
     gene            complement(5852..6511)
                     /locus_tag="PLW95_07385"
     CDS             complement(5852..6511)
                     /locus_tag="PLW95_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22476.1"
                     /translation="MRKKIINLVLFGIAMGYLEAAVVYYLREIYCPSGFFFPFPMPPV
                     KLYLVELGRELSTIVMLTTMSYLIGKGVEILGCFCILFGLWDIFYYVFLYVLLKWPSS
                     LFTWDILFSIPVSWASPVICPVLVSILMIVFGVMILIIKDKKEKIIILNREKILFILS
                     LFFIFISFTIDAFINPKIYLSVKPENFHWGFFSTGVIFFIIVLYNIYKENRTKNKRFS
                     P"
     gene            complement(6508..7416)
                     /locus_tag="PLW95_07390"
     CDS             complement(6508..7416)
                     /locus_tag="PLW95_07390"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOV22477.1"
                     /translation="MVIKKIFLFIFLVSSFVFSTSYNWQNAPSYEEKPILKFALITDT
                     HCTCQGEFKKEPKHIIQIGPYRVHWKNSYYSFSIVEKTIKYIGNNVKPDFIIHCGDIT
                     EYGKKEDMEEVKKILDNSTIPYYVVMGDHDVVGKNNFKDVFGEKYYSFDFNNWHFIII
                     GIFPSDEEISYLKNDLKENSQKPTIIATHRLLICDRFTKFLVKNFTHNTPLLMPKANE
                     ILSIIKEFPQVKIVLSGHIHSNLHYKENNIHFISTSSLIEPPYQFRNFVVYKDKIFVQ
                     TYISKNINSLLNGEWAVFNEEIIYLK"
     gene            complement(7407..7904)
                     /locus_tag="PLW95_07395"
     CDS             complement(7407..7904)
                     /locus_tag="PLW95_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOV22478.1"
                     /translation="MEEIIKILKERRSIRKYQKKEVPEDIIKEIIDCARFAPTAVNVQ
                     PWEFIVVKEEKRRNEIANITDYGKFIADAPVCVAVFCKDTKYYLEDGSAATTYILLAA
                     KGFGLGSCWIAGDKKHYAEKIREYLEVPTGYKLVSLISIGYPAESPKKEKRPLNEVLH
                     KEKWS"
     gene            complement(7966..8190)
                     /locus_tag="PLW95_07400"
     CDS             complement(7966..8190)
                     /locus_tag="PLW95_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010871560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HOV22479.1"
                     /translation="MSILPIVKPKDVIRVAIKLGFVFDRQSGSHAVYYRKKDKRRVVI
                     PIHSKDMKKKTLSGIIRDMGIKIEEFKKLL"
     gene            complement(8187..8414)
                     /locus_tag="PLW95_07405"
     CDS             complement(8187..8414)
                     /locus_tag="PLW95_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015226323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOV22480.1"
                     /translation="MEEYMYTVIIEREQEGGYHAFCPILQGCHTQGETYEETLENIKD
                     AIKLYIESLKSHNETIPVEDDISIKLAKVTL"
BASE COUNT         2703 a         1764 c         1196 g         3134 t
ORIGIN      
        1 ctcatccccg ccttctcccc aacggggaga aggaaataaa tccccctcaa tccccgttgt
       61 gctatctata caaatccact tcttaaaatc tccctctgcc cctctttata aaagagggga
      121 gtatttttgg atgacgcacc aactctgctt tgctaccctt tgcttcctta gtcggttttt
      181 caaaggggga agcatctctc cttcccttat aaagggaagg tcgggatgga tttataaaaa
      241 tgcccttacc ttattttttc aaggacccgg ttctcacatc caaaggaccc ctctgggcgt
      301 agcccaacgg gcgagccttc ggagagaacc aggccctttt atcttattct ttcttaaaat
      361 cccccctcat ccccgccttc tccccaacgg ggagaaggaa ataaatcccc ctcaatcccc
      421 gttgtgccta ttgaaataca taacggcacc aactctgctt tgctaccctt tgcttcttca
      481 gtcggtttgt taaaggggag aactaaaaga gagaacttat gggtaaggga tagttcccct
      541 ttgggagaga ataggtgaga ggtgcctttt cagtcaaagc agttgactaa aaacttaaag
      601 tttttctttc aaaaactttc ctgtatatga ttttgggttt ttggcaattt tttctggcgg
      661 tccttgtgca attacatatc cacctttatc tcctccttca ggtccaaggt ctatgagcca
      721 atcagcatac tttataacct ctaaattatg ttcaattaca ataacacttg accctttttc
      781 aaccaatcta ttcaaaacac caagaagttt ttctatatct gctaaatgaa gtccaacggt
      841 tggttcatca agaatataaa tagtcctttc attccctttt cttgaaagtt ctgctgcaag
      901 tttaattctt tgtgcttctc cacctgataa agtcgttgct ggttgtccta atgaaatata
      961 accaagtcca acatcataaa gtgtttgtaa tttatttttc agatgaggaa aatttttaaa
     1021 aaactcaaca ctttcttcta ctttcatatc aagaatttct gctatatttt tccctttata
     1081 ttttatttca agcgtttctt tgttatacct tgtcccttta catacttcac atggaatata
     1141 aatatcaggt aaaaaatgca tctctatttt tgttatacct tcacctatgc atgcctcaca
     1201 tcttccacct ttaacattga aactgaatct tcctggttta taccctctga ttcttgcttc
     1261 ttctgtattt gcaaaaattt ctcttatata ggtaaacgcg cctgtatatg ttgctggatt
     1321 tgaccttgga gttcttccta ttggtgattg gtcaatttca acaactttat ttatatattc
     1381 aatacctaaa attgcatcat gttcacctgg ctcttctttt gatttatata aaatttttct
     1441 taatgcatta tacaaaacat cttctattaa actacttttc cctgagccag aaacacctgt
     1501 aacacaaaca aatagcccca atggaatttc aacatttata tttttaagat tattatgttt
     1561 tgctccaatg atttttatcc tttttcctgt tggttttctt cttttggaag gtaaagtaat
     1621 ttccctttct cctgaaagga attttccagt aattgaattt ttaaccttta taatatcatc
     1681 tacttttcct tctgcaacaa catatccgcc atgaatacct gctcctggtc caaggtcaac
     1741 aacccaatca gcatttttca tcatttcttc atcatgctct ataactacaa ctgtattgcc
     1801 aaggtctctt aatgatttca atgtatttat caatcttaaa ttatctcttt gatgaagtcc
     1861 tattgttggc tcatcaagaa tataagcaac tcctacaagt cctgaaccaa tttgagttgc
     1921 aagacgaatt ctttcaactt ctccaccaga taaagtatga gtcatcctgt caagtgtcaa
     1981 ataatcaagt ccaacttctt ccataaaatt taatctgctt attatttctt ttaatatctg
     2041 ttttgctatc attttttctc tttcatttaa ttttaaggta ttaaaaaatt cctttactcc
     2101 ctttacactc atagaagtta tatccattat tgattttccc ccaacagtta cagacaacgc
     2161 ctcttttctc aaccttcccc cattacaaac agggcatttt atttctctta tatatttcat
     2221 tatttcttct tttcttcttt cactttctgt ttgcttaaaa agtcgctcta aatttggaat
     2281 aactccttca aaattatatt catcagaacc ataaagcaaa atattaagtt gttcttctgt
     2341 taagtcagaa ggactatcat caagaccctt attaaattga taaagtaatt cccttaacaa
     2401 acttctgtaa tacaaaaaaa tacctctacc acctgtttca tgccatggtc ttattgctcc
     2461 ttttctaaat gtcttatttt tatcaggtat tacaaggtca gggtcaattc tcattaaaaa
     2521 ccccaatcct ttacattcag gacatgttcc ataaggacta ttaaaagaaa acatccttgg
     2581 tgaaagttct tcaaaactta ttccacattt aacgcatcca aaattctcat tgaaaaaaac
     2641 ttccttctgc ccatcatcaa ctataattac acctttccca actttcaatc ccatttcaac
     2701 actttctgct accctacttc tttcttcttc tcctataata aactggtcaa taagaacttc
     2761 tatattatgg attttgtatt tttcaagttt tattttttca tcaacttcat aatattttcc
     2821 atcaactctt accataagaa aaccactttt cttaatttcg tcaaaaactt tgacatactc
     2881 tccttttctt cctcttacaa caggcgctaa aattttcact tcttttgtag gtggaaaaga
     2941 tagaatatta tcaataattt cggaagatga ctgtcttgaa ataatttttc cacatgctgg
     3001 acaatgaggt atcccaattc tcgcaaaaag taatctcata taatcataaa tttcagttgt
     3061 tgttgcaaca atggaccttg gatttgaggc ggcttttctc tgttcaattg caattgcagg
     3121 tggaagtcca aaaatatggt caacatctgg tttcttcatc tgctctaaaa attgtcttgc
     3181 ataagtagat aaactttcaa tataccttct ctgtccttct gcatataagg tatcataagc
     3241 aagggatgac ttaccagaac cagaaacacc ggttattact actaaattat tccttgggat
     3301 gtctatattt atatttttta gattatgttc tcttgctcct ctaatactta taaattttgg
     3361 ttccataatt tattatttta ccctcaaaat ataaataatg ggatatattc aatttacaat
     3421 tttaattaac agaaagtccc gcaaatttta ataattatgc agttaatacc aatttgacaa
     3481 tatacataca gtaaaaatat tttcccctca ccttaaatca ctcgggcgta gtccacaggc
     3541 tccgtaagcc aaaagcgagg accctacgga cgaggggaag ggtgaggggg gtattaattt
     3601 ctattatata tttgatgtaa aaacagtatg aatatcttag tgtccaaaaa tatgggagtt
     3661 taggaaaata gtataaaccc ctttcaaaat gtgaattctt taaatttttt catagttata
     3721 aaaaaacggg attttaggtt ctaacagaag ggagaatata tttagagaag taatgtaagt
     3781 tattgatttt caaaaacttg ttaacttcgt aagttaacag gaaggttaaa aaggaagagc
     3841 attttttaat tttattgctg tttgtaaaac aactggtgcc ttgtttttta atccagggtc
     3901 ttttgattta gcagaaagaa ctatttttac aagcgttgtc tcagtgtcca attcccctgt
     3961 aataatatct ctgcctctgc tatcataata agtaaaattt gaatatcttg tattttctgg
     4021 ctcatcatta ttaacaacaa gttctacttt attatttata tcaattagtg cttcttcatt
     4081 ataaaaattg gaggcatctg gtggtaatgg gtcagaatac caaccaaccc ataaagtttt
     4141 tgtattatct ccttcatcag taagttctct ttcacttgca attctaacaa tcattgtttc
     4201 attttgtgtt tcattataag caacaagggt attataagaa acacatgtat catcataagt
     4261 tccctcatta agagtaattg accttataca agatgcatca gcaagtcccc aaccacctct
     4321 tttaccatga acaattgtat ccatcatttg agaaagtgtt tgttgaattt cactatgtgc
     4381 aacaacaatt tttcttactg ttgacgctct agcaaaaaga acagttataa gaccaaaaat
     4441 aatgacaaga ataaacatag aaatcaaaat ttcaataaga gtaaatccat ttccaacctt
     4501 actttttttc atattttgcc tctcttaaac tattgctttc ctttcaaaaa aatcaactcc
     4561 ttcctttcca aaaggaaggt aggctcgccc gtagggtcca cagaatttct ctgggctatg
     4621 cgcgagggga tggatttctc cctttaaaac aacccatttc ttgaaatata aatttctctt
     4681 cttgataaaa ccgcattccc ttttcttctt aaaacataaa caataactct taaacccatc
     4741 gctttgtcat aatcaggaga agtcgcccag ttagataggg gtgtagaagt gtcattacca
     4801 tatccaatta catatctttc aattttaaat ctatcctgaa attcaggagg agtaaaatca
     4861 gttcctgttc caatattaat tgaattttca tcctcaattt catcaactat tttcccgtac
     4921 tcctccggtt tctgttcacc ccatctatca ctataaacag gaggaaattc aaaattttta
     4981 acttcttcaa tactatattg agaaagagca tttgctatca ttatttcatt tgctttattt
     5041 acaacattca tatttgatga cattaaaagt aaaactattc ctataacaat agcaattaaa
     5101 aatatagaaa tgaccatttc aagaagagta aatccatttt tataattttt cattttgctt
     5161 ttcgcccata tttattttac catatttatt tttctttttc accatttttt tacttcttat
     5221 ttactttttt caatgttttt ttctacttgg aggtaaattt tttattctct caaaatcccc
     5281 cctcatcccc accttctccc cgctggggag aaggataaaa tctccccctc aaaatctccc
     5341 tttatccccg ttgtagttat taaatccctc ttaatctccc ttttataaag ggagaatttt
     5401 ggggaacaag caccaactct gctttgccat gcctaccttc cttagtcgtt ttacaaaagg
     5461 ggaaattaca ataaatccct tccttttata aaggcctcgc ccgtaggctg tagcctacag
     5521 gctacggccg agggaagggg ggctcgcccg tagggctacg cccgaggggc tggatttatt
     5581 aaaccccgta agcccaactt agcacttctg cgtagggctg gttcgtatgc tatattttaa
     5641 actgcctgtg tagaacaatc tcccctcatc cccgccttct ccccaacggg gagaaggatg
     5701 aaatccccct ctctccttct cccctctccc cgctggggag aggctcgccc gtagggctac
     5761 gcccgagggg ctggggtgag ggggaaattt atctccttcc cttaataagg catgcccgtc
     5821 tatctttgta gaggggaagg gaaggtgagg gtcagggtga gaatctctta ttcttggttc
     5881 tattttcttt atatatatta taaagaacaa taataaaaaa aataacccca gtagaaaaaa
     5941 atccccaatg aaaattttct ggtttcacag agagatatat tttgggattt atgaaagcat
     6001 caattgtaaa agaaataaaa ataaaaaata aacttaatat aaacaaaatt ttctctctat
     6061 tcaaaataat aattttttct tttttatctt taattatcaa tatcattact ccgaaaacaa
     6121 tcataagaat agaaactaaa acagggcata ttacaggaga tgcccatgaa acaggaattg
     6181 aaaaaaggat atcccaggta aataatgatg aaggccattt taaaaggaca taaaggaata
     6241 cataataaaa aatatcccat aaaccaaata aaatacaaaa acaccctaaa atttcaacgc
     6301 cttttccaat aagataactc attgtagtta acataactat tgtagaaagt tctcttccaa
     6361 gttcaactaa atatagtttt actgggggca ttgggaatgg gaagaagaac ccagaagggc
     6421 aataaatttc tcttaaataa taaacaactg ctgcctctaa atatcccatt gctatcccaa
     6481 aaagaaccaa atttataatt ttttttctca ttttaagtaa ataatttctt cattaaagac
     6541 agcccattct ccatttaata aggagttgat gttttttgat atatatgtct ggacaaaaat
     6601 tttatcttta taaacaacaa aatttctgaa ttggtaaggt ggttcaatta aggaagaagt
     6661 tgaaataaaa tggatattgt tttctttata atgaagattt gaatgtatat gtcctgataa
     6721 cacaattttt acctgtggaa actcttttat aatagataga atttcatttg cttttggcat
     6781 caataaaggt gtattatgag taaaattctt tacaagaaat tttgtaaatc tatcacatat
     6841 aagtaatcta tgagttgcaa ttattgttgg tttttgagaa ttctctttta aatcattttt
     6901 aagataagat atttcttcat cagatgggaa aataccaatt attataaaat gccaattatt
     6961 aaaatcaaat gaataatatt tttccccaaa tacatctttg aaattattct tcccaacaac
     7021 atcatggtct cccattacaa cataataagg aattgttgaa ttatcaagaa ttttttttac
     7081 ttcctccata tcttcttttt ttccatactc tgttatatct ccacagtgaa ttataaaatc
     7141 tggttttaca ttatttccaa tgtattttat ggttttttca acaattgaaa aagaataata
     7201 ggagtttttc cagtgaactc tataaggtcc tatttgaata atatgttttg gttctttttt
     7261 aaattctcct tgacaagtgc aatgagtatc tgttataaga gcaaatttca aaattggttt
     7321 ttcttcataa gaaggagcat tttgccaatt atatgaagta gaaaaaacaa aagatgaaac
     7381 aagaaaaata aatagaaata ttttttttat gaccattttt ctttatggag aacttcattc
     7441 aatggtcttt tctctttttt aggactttct gctggataac caattgaaat cagtgataca
     7501 agtttatatc cagttggtac ttctaaatat tctcttattt tttcagcgta atgtttttta
     7561 tcacctgcta tccaacaact tccaagtcca aaaccttttg cagcaagtaa aatatatgtt
     7621 gtggctgctg acccatcttc taaataatat tttgtatctt tacaaaaaac agcaacacaa
     7681 acaggggcat ctgcaataaa tttcccataa tcagttatat ttgcaatttc gtttcttctc
     7741 ttttcttctt tcacaacaat aaattcccat ggctgaacat ttacagcagt tggtgcaaat
     7801 ctcgcacaat caattatttc tttaataata tcttctggga cttccttttt ttgatatttc
     7861 cttatacttc ttctttcctt caaaattttt ataatttctt ccatttttct cctcctctat
     7921 cattctattt tccaagtgca tagcaaaaat atatgtcaac ttcattcaaa gcaatttttt
     7981 aaattcctct atttttattc ccatatctct tataattcca gataatgttt ttttcttcat
     8041 atcctttgaa tgtataggaa ttacaactct ccttttgtct ttttttctat aataaacagc
     8101 atgacttcca gattgtctat caaagacaaa acctaatttt atagcaactc taattacatc
     8161 ttttggtttc acaattggta aaatactcac aaggttacct ttgctaattt aatagaaata
     8221 tcatcttcta ctggtattgt ctcattatga cttttaagag attcgatata aagttttatt
     8281 gcatccttta tattttcaag tgtttcctca tatgtctccc cttgcgtgtg acatccctgt
     8341 aaaatagggc aaaaagcatg gtacccacct tcttgctctc tttctattat aacagtatac
     8401 atatattctt ccatttttct cctcctctat cacttactga tactgccaaa attggctaat
     8461 taaattcact cccgttgtgc tatctggaag acgataacgc accaactctg agaaacaagg
     8521 taagcaactt cagtcggttt ataaaagagg ggaacattaa atcccccctc atccctacct
     8581 tctccccagc ggggagaagg atgaaatcct cctcaatccc cgttgtacct attgaaatac
     8641 attacggcac caactctgct ttgctaaccc ccgctacttt agtcggttta ctaaaggggg
     8701 aagtttttct ccttccctta taaagggaag gtcgggatgg attttattaa accccgtaag
     8761 cccaacttag cacttctgcg tagggctggt tcgtatg
//
LOCUS       DAOOVW010000062         8491 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_5126_length_8491_cov_8.153864, whole genome shotgun sequence.
ACCESSION   DAOOVW010000062 DAOOVW010000000
VERSION     DAOOVW010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8491
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5126_length_8491_cov_8.153864"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="PLW95_07410"
     CDS             <1..682
                     /locus_tag="PLW95_07410"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF013641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOV22481.1"
                     /translation="GVNLTIPHKKDAFFLVNEVGEEAKLLKVINTIKNEDGKLTGYIT
                     DGKGFVRSIKEDGKMNLKGKKIYLIGAGGTAWAISGCLIKENIKGIFITNRTLEKAIL
                     LKKHLTKNFNFNNVEIIPFEKRNDINFDEIDVIINTTSVGMKENDPILIEEKFIKKKF
                     FIYDVVYNRKTELIKTAEKLGLPYVNGLSMLVYQGAISFEIWTGEKAPVDIMKKSLYN
                     FLKNGEKK"
     gene            679..1836
                     /gene="aroC"
                     /locus_tag="PLW95_07415"
     CDS             679..1836
                     /gene="aroC"
                     /locus_tag="PLW95_07415"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOV22482.1"
                     /translation="MRFLTSGESHGKCLVGILEGMVANMDISISEINKELERRQRGYG
                     RGKRMEIEKDKVEIISGIRNGKTTGGPIGIIIKNKDWQNWKEIMDIEKGHGRENFIPR
                     PGHADLPGFIKYKYTGLRNVIERSSARETAMRVAIGNICKQFLKDFNINIFSRVIQVG
                     KVKDKSTWDEVKRNYALIEKSEVRNFIKEKEIIKEIEKARKNGDTIGGIFEIIIEGVP
                     PGLGSYVQWDRRLDAKISFYLMSIPAVKGINIGIGFEGIEKFGSQFHDEIYYDESKKI
                     YRKTNNAGGIEGGITNGENIIIRCALKPIPTLKKPLNSVNIKTLKEEISIYERSDICV
                     VFPASVIGENILSFLIADEILNKFGGDTIEDVKTNYNFYLKSISKYWSNKK"
     gene            1833..2363
                     /locus_tag="PLW95_07420"
     CDS             1833..2363
                     /locus_tag="PLW95_07420"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOV22483.1"
                     /translation="MKKNIILTGFMGTGKTLIGNIVADKLNMEFIDTDKVIEEREKDR
                     IARIFQVKGEEYFRKLEEKVINEICKKENCVIATGGGAIIREKNYNNMKKSGIIICLE
                     AEPSIILLRTSTTEDRPLLLKNKDAISTIRYLINSRKKYYDKADYKIDTTSLSPEQAG
                     EKIIEIWKKENEKNRN"
     gene            2344..3414
                     /gene="aroB"
                     /locus_tag="PLW95_07425"
     CDS             2344..3414
                     /gene="aroB"
                     /locus_tag="PLW95_07425"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOV22484.1"
                     /translation="MKKIEIKIEEKKKSICFIDIPFEKIPEKLKSNGDKFLIISDKNV
                     YPLFGEKLRSVISKNGDVYKFILKSGENEKNLKNIEKILHYCYENNFSRDDTILALGG
                     GVISDISGFVSSIYMRGINFITIPTTLLAQVDASIGGKTGVNFYNKKNLIGSFYQPEF
                     IFINFETLKTLPEREMKQGIAEIIKYGVIKNKKIFELLEKNKENIKKYLPEIVEKCVK
                     IKGDIIKKDEKEKTGLREVLNFGHTIGHAIEVLDNYKFNHGEAIANGMVYETFIAFKL
                     GFCEKATYERIKNIVSSFNFPEFKGKNNIDKIIENLWYDKKVRKGNLRFVLPEKIGKV
                     KTGVVVKEEIIKKTFLEEENNG"
     gene            3407..4483
                     /gene="pheA"
                     /locus_tag="PLW95_07430"
     CDS             3407..4483
                     /gene="pheA"
                     /locus_tag="PLW95_07430"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012991967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOV22485.1"
                     /translation="MDEIERKREEIDQIDKKLLELLDLRAEKVKEITDIKNKNNKPIF
                     DPSREKRIIEKHIEKRFKYLKKEDIELIMSTIFKIYRGFFKPLNISFLGPEGTFTHQA
                     ALRKFGNKCNFISAKTVEDIFKEVEKDRVDYGVVPIENSVEGVVTYTIDMFLESNLKI
                     TSEILLEIHHYLLSNESSLKKIKKIYSHPQAFAQCRKWLLSNLENVELIETESTTNAV
                     KKAKKMKNTGAIGSEIASSLYKLPVLAEKIEDFTENITRFLVIGNDSPKMTGKDKTSI
                     IFSIKDKVGALYDSLYPFKEKGINLTRLESRPSKKRAWDYVFFVDFIGHCDEDKVQSA
                     LEELEKNCVFLKVLGSYPLENKEK"
     gene            complement(5056..5496)
                     /locus_tag="PLW95_07435"
     CDS             complement(5056..5496)
                     /locus_tag="PLW95_07435"
                     /inference="COORDINATES: protein motif:HMM:NF025126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOV22486.1"
                     /translation="MCPGGQELHHIGRSKHREVVYDTYGNVRECKKCEYYGVCTTSPR
                     GRHISISEHEGLFKEMREKLSTEEGKEIYGIRKITLEPVFGNLSQNLGFREFVLRGKE
                     KVRGEFSLMCTAHNLLKIAKFVRKSGKKLGELLREREVLSIGAT"
     gene            complement(5587..5805)
                     /locus_tag="PLW95_07440"
     CDS             complement(5587..5805)
                     /locus_tag="PLW95_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22487.1"
                     /translation="MANDVTEKQSDCCQLIPMVEEVLKNIEKVVPEKREKINIVADSG
                     YSSGKNFASLERKEYKEKIEAYIPDVIT"
     gene            complement(5843..6028)
                     /locus_tag="PLW95_07445"
     CDS             complement(5843..6028)
                     /locus_tag="PLW95_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22488.1"
                     /translation="MLPEGIREKEKRIKEIEKEIKKLEESKKKLREGKKKKINLTDAS
                     VEFQKDKTRQDKENTWL"
     gene            complement(6125..6469)
                     /locus_tag="PLW95_07450"
     CDS             complement(6125..6469)
                     /locus_tag="PLW95_07450"
                     /inference="COORDINATES: protein motif:HMM:NF017416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOV22489.1"
                     /translation="MPSVGNPSYHPALMIKIWFYGYATKVYSSRKIEEKLRSDIAFIY
                     LSGMEKPDFKTISEFRRKNINALRDTFVEILQICNRIGMTRLGTISIDSKVMKGNASS
                     ERTYTEEEIIKE"
     gene            6741..7061
                     /locus_tag="PLW95_07455"
     CDS             6741..7061
                     /locus_tag="PLW95_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22490.1"
                     /translation="MRAFYGKLYGVVTENNGNYGFVHLNRPNGSDRISKARLQTIKFP
                     VFSLSDTKEIKSLFLKNSGVRKEVSRPLGYDRRLLPYLWLSPSPSFSKGENKMLKKQS
                     YRYD"
     gene            7111..7323
                     /locus_tag="PLW95_07460"
     CDS             7111..7323
                     /locus_tag="PLW95_07460"
                     /inference="COORDINATES: protein motif:HMM:NF013698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22491.1"
                     /translation="MEAFPFENVSFPGMKKLLDKTLIYGKNPIFVFEATNVFWKPLYF
                     HITSSGYQCFTVSASQTGAYRKRKNP"
     gene            7295..8212
                     /locus_tag="PLW95_07465"
     CDS             7295..8212
                     /locus_tag="PLW95_07465"
                     /inference="COORDINATES: protein motif:HMM:NF014430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOV22492.1"
                     /translation="MLIVKGRIRKTKTDKIDCKEILELYLKGEAQPTKILSQHLTSLK
                     ELTRLYTHLVDTKTNLSYRIREYIYQLFPEWENTFKDIFCKTILYLLEKQFSSPQLLS
                     KTRRDKLAHIIRKVSHGKFGLPVVNQLKENAQNSFGIPLIQQALSLNLSLLVKSINSL
                     DNILIPLENNIKNIFLQVPQHLLTVPGVAVIPASSFISELGNPNDFSNADKVIAWFGL
                     DIVWKSSAGKGKGYHVSKTGTPYGRKWVYVAAGEFVRHFPPAKQKYLKIFKEHHIHKS
                     SLIPICADLTKILFAMYRDGTTFDPSRYH"
BASE COUNT         3115 a         1301 c         1278 g         2797 t
ORIGIN      
        1 aggggtaaat ttaacaatac cgcataaaaa agatgctttt tttcttgtta atgaggttgg
       61 tgaagaggca aaactactta aagtaataaa tacaattaaa aacgaagatg ggaaattaac
      121 tggatatata acagatggaa aaggctttgt aaggtcaata aaagaagatg ggaaaatgaa
      181 tttaaaagga aaaaaaatat atttaattgg tgctggtgga actgcatggg caattagtgg
      241 ttgtcttatc aaagaaaata ttaaaggtat ttttattact aacagaacct tagaaaaggc
      301 aattttactt aaaaaacatc ttacaaaaaa ttttaatttt aacaatgttg aaataatacc
      361 atttgaaaaa agaaatgaca taaactttga cgaaatagat gtaattataa atacaacatc
      421 tgttggtatg aaagaaaatg acccaatttt aatagaagaa aaatttataa agaaaaaatt
      481 ttttatatat gatgttgttt ataatagaaa aacggagttg attaaaacag cagaaaaatt
      541 aggtcttcca tatgttaatg gtttatcaat gcttgtttat caaggagcaa tatcttttga
      601 aatatggaca ggagaaaaag cccctgtgga tataatgaaa aaatcgttat ataatttcct
      661 taaaaatggg gagaaaaaat gagattttta acaagtggtg aatcacacgg aaaatgttta
      721 gttggaattt tagaaggaat ggttgcaaat atggatattt caatttctga aattaataaa
      781 gaacttgaaa gaagacaaag aggatatggg cgtggaaaaa gaatggaaat tgaaaaagat
      841 aaagtagaaa ttatctctgg cataagaaat ggaaaaacaa caggtggccc aataggaatt
      901 attattaaaa ataaggactg gcaaaattgg aaagaaatta tggatattga aaaagggcat
      961 ggaagagaaa attttattcc aagaccagga catgcagacc tgcccggatt tattaaatat
     1021 aaatatactg gtttaagaaa tgttattgaa agaagcagtg caagagaaac agcaatgaga
     1081 gttgcaattg gaaatatctg caaacaattt ttaaaggatt ttaatataaa tattttcagc
     1141 agagttatac aggttggtaa ggttaaagat aaaagtacat gggacgaggt aaaaagaaat
     1201 tatgctttaa ttgagaaatc ggaagtaaga aattttatta aagaaaaaga aataataaaa
     1261 gaaatagaaa aagcaagaaa aaatggagat acaattggtg gtatttttga aattataatt
     1321 gaaggagtgc caccaggact tggcagttat gtccaatggg acagacgact tgatgctaaa
     1381 atttcatttt atttaatgag tatcccagcg gtaaaaggaa taaatatagg tatcggtttt
     1441 gaaggaattg aaaaatttgg ttctcaattt cacgatgaaa tttattatga tgaaagtaaa
     1501 aaaatttata gaaaaacaaa taatgcaggt ggaatagagg gtggaattac aaacggggaa
     1561 aatataatta ttagatgtgc attaaaacca attccaacac tcaaaaaacc attgaattca
     1621 gtaaacatta aaacattaaa agaagaaata tcaatttatg aaagaagcga tatttgtgtt
     1681 gtatttcctg catcagttat tggtgaaaat atactctcct ttttaattgc tgatgaaatt
     1741 cttaataaat ttggaggaga tacaattgaa gatgtaaaaa caaattataa tttttatctc
     1801 aaatcaatat caaaatactg gtcaaataaa aaatgaaaaa aaatattatt ctgactggat
     1861 ttatgggaac aggtaaaaca cttataggaa atattgtcgc tgataaattg aatatggaat
     1921 ttatagatac tgataaagta attgaggaaa gagaaaaaga tagaattgcg agaatttttc
     1981 aggttaaagg agaagaatat tttagaaaat tggaagaaaa agtaataaac gaaatatgta
     2041 aaaaagaaaa ttgtgtaatt gcaacaggtg gaggagcaat aataagagaa aaaaattata
     2101 acaacatgaa aaaaagtgga attattatct gtcttgaagc agaaccatct ataattcttt
     2161 taagaacatc aacaacagaa gatagaccac tactcttaaa aaataaggat gcaatttcaa
     2221 caataagata tttaataaat tcaagaaaaa aatattatga caaagcggac tataaaatag
     2281 atacaacttc tctttcacca gaacaagcag gcgaaaaaat aattgaaata tggaaaaaag
     2341 aaaatgaaaa aaatagaaat taaaattgaa gagaaaaaaa aatcaatatg ttttattgat
     2401 attccttttg aaaaaatacc tgaaaaacta aaatcaaatg gtgataaatt tctaattata
     2461 agtgataaaa atgtctatcc tctttttgga gaaaaattaa ggtcggttat ttcaaaaaat
     2521 ggagatgtct ataaatttat tttgaaaagc ggggaaaacg aaaaaaacct taaaaatatt
     2581 gagaaaatac ttcattattg ctatgaaaat aatttttcaa gagatgatac aatacttgct
     2641 cttggcggag gagttataag tgatatatct ggctttgtat cttctattta tatgagagga
     2701 ataaatttca tcacaatacc caccacttta cttgctcagg ttgatgcctc tattggtggg
     2761 aaaacaggag taaattttta caataaaaaa aatcttattg gaagtttcta tcaaccagaa
     2821 tttatattta tcaactttga aacattaaaa acattacctg aaagagaaat gaagcaggga
     2881 attgctgaaa taataaaata tggtgttata aaaaacaaaa aaatttttga attgcttgaa
     2941 aaaaataaag aaaatataaa aaaatatcta ccagaaattg ttgaaaaatg tgtcaaaata
     3001 aaaggtgata ttattaaaaa agatgaaaaa gaaaaaacag gacttcgcga agtattaaat
     3061 tttgggcata caattggaca tgcaattgag gtattagata attataaatt caatcacggt
     3121 gaagcaattg caaatgggat ggtatatgaa acatttattg cctttaaact tggcttttgt
     3181 gaaaaagcga catatgagag aataaaaaat attgtaagta gtttcaattt tcccgaattt
     3241 aaaggtaaaa ataacataga taaaataatt gaaaatttat ggtatgataa aaaagtgaga
     3301 aaagggaatt tgagatttgt tctaccagaa aaaattggaa aagttaaaac aggagtagtt
     3361 gtaaaagaag aaataataaa aaaaacattt ttagaggaag aaaataatgg atgaaattga
     3421 aagaaaaagg gaagaaattg accagattga taaaaaactt cttgaacttt tagaccttcg
     3481 ggctgaaaaa gtcaaggaaa taactgatat taaaaataaa aacaataaac caatttttga
     3541 cccttcaaga gaaaaaagaa ttatagaaaa acacattgaa aaaagattta agtatctaaa
     3601 aaaagaagat attgagttaa taatgtccac aattttcaaa atttatagag gattttttaa
     3661 accattaaat atatcctttc ttggtccgga agggactttt acacatcagg cagcactcag
     3721 aaaatttggt aataaatgca actttatttc agcaaaaaca gttgaggata tttttaaaga
     3781 agtggaaaaa gatagagttg attatggtgt tgttccaatt gaaaattctg tggaaggagt
     3841 ggttacatat acaatagata tgtttcttga aagcaattta aaaataacat cggaaatttt
     3901 acttgaaatt catcattatc ttctttcaaa tgaaagttca ttaaaaaaaa ttaaaaaaat
     3961 ttattctcat cctcaggcat ttgctcaatg tagaaagtgg ttattatcaa atttagaaaa
     4021 tgttgaattg atagaaacag aaagcaccac aaatgctgta aaaaaagcga agaaaatgaa
     4081 aaatacagga gcaattggtt ctgaaattgc ttcatctctt tataaattac ctgtcctggc
     4141 tgaaaaaata gaggacttta ctgaaaacat aacaagattc cttgtgatag gaaatgactc
     4201 tccaaaaatg acaggaaaag ataaaacatc aataattttc tctataaaag ataaagtcgg
     4261 tgctctttat gattctttat atccatttaa agaaaaagga attaacttaa cacgattaga
     4321 atcaagacca agtaaaaaaa gagcatggga ttatgtattt tttgttgatt ttattggtca
     4381 ttgtgatgaa gataaagttc aaagtgcatt agaagaactt gaaaaaaatt gtgtattttt
     4441 aaaagtttta ggttcttacc cattagaaaa caaagaaaaa tagtaaaatg taaaactaaa
     4501 agcggaaaag ttaaagatac aacttaaaat ttaaaaaatt tttgtccttt gttcttcgtc
     4561 cgctaaaaag cgcatcggac gctaaaatac gcgttttgaa tccgcctcat tcgatacaaa
     4621 acatggcgaa tccgctgcga tttttggcgt aaagatggac tggcagtact ttccagggga
     4681 agaagcaatt ttctcccttt cttaaaccga ctgaagttgc ttaccttgtt tctcagagtt
     4741 ggtgtttaac gttttttcca agcacaacgg agaggaattt tacgtttccc ccttttagaa
     4801 aggcctgtcc gacgcgcatt ttggcggagg gattaagggg gatttcagca agcacaacgg
     4861 ggttaggaat ctgtgtcata aatagaaaaa ataattattt tttttcaaaa tgttcaacaa
     4921 aaacctttaa aaattatact tatcttttta tttctttttt gttctaatac atctatttac
     4981 tccaacttcc ttttttctaa ctattattaa agaaaaaatt cctaattttc tttatttcta
     5041 tcttcctatt tttttctatg ttgcacctat tgataatact tctctttctc ttaacaactc
     5101 tcctaatttc tttccactct ttcttacaaa ttttgctatc ttcaaaagat tatgggctgt
     5161 acacatcaat gaaaattccc ctcttacttt ctctttccct cttagtacaa attccctaaa
     5221 tcctaaattc tgacttaaat tcccaaaaac tggttctaat gttattttac gtatcccata
     5281 tatctctttc ccttcttctg ttgataactt ctccctcatt tctttaaaca atccttcatg
     5341 ctctgatatt gatatatgtc tacctcttgg gctggttgta cataccccat aatactcaca
     5401 cttcttacat tccctaacat tcccatatgt atcatatact acttccctgt gtttgcttct
     5461 ccctatatga tgcaactcct gtccacctgg acaaattaat atcttttcct ttttattata
     5521 tataaaatta cttttatcaa atctattatc ctgtttatgt gaactaccta tccctttcct
     5581 gttttgttat gttattacat ctggtatata tgcctctatt ttctctttat attccttcct
     5641 ttctaatgaa gcaaaattct taccactact atatccacta tctgcaacta tatttatctt
     5701 ttccctcttc tcaggtacta ctttttctat attctttaat acttcttcta ccatcggtat
     5761 caattgacaa caatcactct gcttctctgt tacatcatta gccactatca cttactcctt
     5821 tgaatctact gctgtatgtg ctctataacc atgtattttc cttgtcttgt cttgtcttgt
     5881 ctttctgaaa ttctaccgat gcatctgtaa gatttatttt cttctttttc ccctctctta
     5941 acttcttctt actttcttcc aatttcttta tctccttttc tatctccttt atcctttttt
     6001 ctttctctct tattccttcc ggaagcacat tcccactttt ttcactccca tatctcttgt
     6061 cttcttcttc atctacctgc tctgccctct ctaaatactc ctttatcgct ccttctaatt
     6121 cctcctactc ctttattatc tcttcttctg tatatgtcct ttcactactt gcattccctt
     6181 tcattacttt gctatctatt gatatcgtcc ctaatcgtgt catccctatt ctgttacata
     6241 tctgtaatat ctctacaaat gtatccctta acgcatttat attcttccga cgaaattctg
     6301 atatcgtctt gaaatccggc ttctccatac ctgataagta tataaacgct atatcacttc
     6361 taagtttctc ctctattttc cttgaactat atacctttgt cgcatatcca taaaaccata
     6421 tctttatcat caatgccgga tgatatgacg gattccctac actcggtata ttcttataat
     6481 aagaacttaa atctatctca tctactacat gtgctaaatg tccttcttct aaataatccc
     6541 ctaccgatgc aggaaataaa agctgactct ttaaaggttt taattttaag gaagggtaaa
     6601 gaaagcggat atttttcctg ctctttataa gagcgagacg gagtttgctt ctttctcctt
     6661 cttgtttttt gtgttttaat atatcagtaa acagggaaaa gagagtagca cgttttcctt
     6721 accctttaaa agggaataaa atgagagcat tctacggaaa actttatgga gtagttactg
     6781 aaaataacgg aaattacggc tttgtccatt tgaatagacc aaatggctct gaccgaatat
     6841 caaaagcaag gttacagacc ataaagtttc ctgttttctc tttatctgac acaaaagaga
     6901 ttaaatctct tttcctaaaa aattcaggtg tcagaaagga ggtgtctcgc ccattgggct
     6961 acgaccgacg gctactccct tatctctggt tatcaccttc tccttctttt tcaaaaggag
     7021 aaaataaaat gttaaaaaaa caatcttatc gttacgattg accctggtaa atatatttgc
     7081 caggcactta tctgtcaaaa tggcaaaatt atggaagctt tcccatttga aaatgtctct
     7141 ttccctggaa tgaaaaaact tctggataaa acattgattt atggaaaaaa tcctatattt
     7201 gtctttgaag caactaatgt cttctggaaa cctttatatt ttcacattac ttcttctgga
     7261 tatcaatgtt ttaccgttag tgcttctcaa actggtgctt atcgtaaaag gaagaatccg
     7321 taaaactaaa actgataaaa tcgactgtaa agaaattctg gaactatatc ttaaaggtga
     7381 agcacagcca actaaaattc tatcacaaca tttaaccagt ttgaaagaac taactagatt
     7441 atatacccat ttagttgata ctaaaacaaa tctctcttat cgcatcagag aatatattta
     7501 tcaactattt ccagaatggg aaaatacctt caaagatatc ttctgtaaaa caatccttta
     7561 tttgctggaa aaacaatttt cttctcctca attattaagc aaaactagaa gagataaact
     7621 tgcacacata ataagaaaag tcagtcatgg aaaatttggt ctgccagtag ttaaccaact
     7681 taaagaaaat gcccagaata gtttcggtat ccctcttatt caacaagcac tttctcttaa
     7741 tctctctctt ctggttaaat ctatcaactc tttggacaat attcttatcc cattagaaaa
     7801 taatatcaaa aatatttttt tacaggtgcc tcaacatcta ttaactgtcc caggagttgc
     7861 tgttattcca gcatcttctt ttattagtga actgggtaac ccaaatgatt tctctaatgc
     7921 tgataaggtt attgcctggt tcggattaga cattgtttgg aaatcttctg ccggcaaagg
     7981 taaaggttat catgtctcca aaacaggtac tccttacggc agaaaatggg tatatgtcgc
     8041 tgctggtgaa ttcgtcagac atttcccacc agcaaaacaa aaatatctca aaattttcaa
     8101 agagcatcat atacataaat catctttaat acctatttgt gctgacttaa ctaaaatatt
     8161 atttgctatg tatcgggatg gaactacttt tgacccatct cgataccatt aactgaaaat
     8221 caaggagtta aggtgataac taactgtttt tatagttttc aaagttcatt tccttttacc
     8281 acttttttgt ggcacggtgg gactgttgtc tctttttcat cttttataat ttaccccttg
     8341 actcctcatt ggaaatctcc cctttccttt tttttacttc tttactccct attattcttt
     8401 attatacaaa attatcagga ataattccaa tctttttcgt ctttcctcaa aataaaatct
     8461 attcgaaata taatttaaat taagcatttt t
//
LOCUS       DAOOVW010000063         6858 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_6539_length_6858_cov_9.331177, whole genome shotgun sequence.
ACCESSION   DAOOVW010000063 DAOOVW010000000
VERSION     DAOOVW010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6858
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6539_length_6858_cov_9.331177"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..977
                     /locus_tag="PLW95_07470"
     CDS             153..977
                     /locus_tag="PLW95_07470"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOV22493.1"
                     /translation="MKKSVNIGVIGSMDFEKGLSIIKDAGFDAVELNLSGVLSMESSE
                     EEVKKVKEKVNSKGLEIASLLAGAFWQFPLTSDNQQKRQSGEKLLLKSIEICKYLETD
                     AVLVVPGVVGTVWESEERVKYDIAYKRSQESIKKCVELAEKNNIYICVENVWNKFLLS
                     PLEMKNFVEEIGSEYVGVYFDVGNILTIGYPEDWIRILGEKIKRIHLKDFKLSVGNIN
                     GFCNLLEGDVNWKEVISALREIKYDGYLTAEYGPYKFFPETIIYNLSLAIDKIIGG"
     gene            983..2125
                     /locus_tag="PLW95_07475"
     CDS             983..2125
                     /locus_tag="PLW95_07475"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOV22494.1"
                     /translation="MTSKERIYDVLLGRIPDRVPVSLYKIDPFDENSFWAQHKSFSKL
                     LETARNLQDTFFFYKLKTGFFFSSPELLEIKVEEYQDTPISKTIKITVITELGPLTRI
                     ARTSNMSMHQWVQKPWIESEKDIHKFLSLPYSPYTPDISEFYKLQEKIGDKGIIVVAL
                     PDPLGVVGFLFAPGDFSKFAIDYPDLIHKLLDKIYERLINIYTFISKNVENVIIRIRG
                     AEYATPPILPEEYFNIKKAFWDFVIRYDTNLIEILKKGKKNYICYHWHDHIDDLISYV
                     MKLGIHIIEPISNTFEKPNFILKIRKIVGDEVVIMGGVDAIDMEFKTPEEITQLVKET
                     IIQGGRKGRFILIPSDIPESAPLTPELERNYITFLQAAIQYGKYPL"
     gene            complement(2132..2926)
                     /locus_tag="PLW95_07480"
     CDS             complement(2132..2926)
                     /locus_tag="PLW95_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOV22495.1"
                     /translation="MSKIIDFHTHAFPDVVAEKAIPLLEKEGGIKARHDGKIKSLLSS
                     MDKYKIDKSVICSIATKVSQYNSILNWSKEIRNERIIPFLSFHPDDKEWEKILDEIKK
                     NNFKGIKLHPYYQNFNLDDKKMFDIYAKIEEVDLILVSHTGFDFAFERIRKADPEKII
                     KIKNAFPSLKLVTTHLGAWEMWDEVEKFIVGKEIYMEISFSLQFLDEDKAKRIILNHP
                     PEYILFGTDSPWADQGETIENLKKLKLSSKLEEMIFYNNASKLLQL"
     gene            complement(2919..3572)
                     /locus_tag="PLW95_07485"
     CDS             complement(2919..3572)
                     /locus_tag="PLW95_07485"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOV22496.1"
                     /translation="MEKLLKEGEVNKILCIGDSITDCGRRDAFYPYGNGYVRIFRDLL
                     ISTFPEKKFEIINKGIGGNTVLDLHNRWEDDVIYNKPDILTILIGINDLHRVLGKNEL
                     LEEYIPESFRKWYNNILQRTVEKLDCKIILMEPFYITIDKTDSFRGTVLKEIENYIQI
                     VQEMSNKYKTYLINLHRIFQHHLEYRESETFAPEPVHPNQTGHTIIANEILKIFKNE"
     gene            3625..4458
                     /locus_tag="PLW95_07490"
     CDS             3625..4458
                     /locus_tag="PLW95_07490"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOV22497.1"
                     /translation="MSKIKLIAVDLDGTLLTDKREIHRKNKYYLKIFSKKGGIVVLSS
                     GRMSNSVSPFSDKLGIDCPLIVYNGAMARLKKSENRKIIFHKPLPSKYSDYIIDYCLK
                     NKFLLNFYINDKLYSQNDPGLKKYALIYSTQTNSKYTFLDNLAKLKGKSPTKLILITD
                     SKNKDIERTRDYQYKFFKRIFKDKLKIVRTNPEYLEFMNKNVDKSVALKKIAQYYKVK
                     KEEIISFGDGENDIEMMCLCRFSVSPSNAKEDVKKIATISLKYSNNDGFIGEFLKILD
                     F"
     gene            complement(4447..5880)
                     /locus_tag="PLW95_07495"
     CDS             complement(4447..5880)
                     /locus_tag="PLW95_07495"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOV22498.1"
                     /translation="MIVKKDREEIISYLEDSSNFKGGKAEVVFIPENEDEIVSILKES
                     SKNKFPLTISGGGTGTVGGRIPVEGGIISLEKFNKIKFINKENKTACLSAGVIVDEFL
                     KELDKYSLFYPPFPTERTAFIGGNIATNASGEYSFRFGPTRKYVKKIRMIRSNGEIFE
                     IKRGDVFEKDNFIDYGCFKIPLPSYRTPDVKCSAGYYSQKGMDGIDLLIGSEGTLGII
                     TEIDVSLIEKLPERFIIVLFVGNKENIPEIIKKIKSEKEFLQLFSLEFFDSKALEFLK
                     SDFPEIPDNTCAIYCEGVSEIEKMERWAEIAEEIKVVETMIGEDKQNYEKLIDFRHRL
                     PDNVNAYFKKIGSVKVAVDAAVPEDKFPLMYNFYTEIIKQNTDLHMILFGHIGENHLH
                     FNLFPKDEKQKEKAWRIYEEAVKKAVSFGGTGFAEHGIGKLKHKYLEIMYGKRGILDM
                     VRIKKIFDPACILSLDNIFPKEYLLEI"
     gene            complement(6047..6346)
                     /locus_tag="PLW95_07500"
     CDS             complement(6047..6346)
                     /locus_tag="PLW95_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22499.1"
                     /translation="MKIKLAQVYTTDKQIEGNIRINLGEGELKDVKFKFEISNKENNY
                     LTEIKADGNNYVKFGINPVNLTAGEYLLKVSAIKGQKEIGSCEEKIHIIKSHFQF"
BASE COUNT         2371 a         1050 c          888 g         2549 t
ORIGIN      
        1 gatttaccta tacctgaata taatacatag ttataaaaaa cgggatttta ggttatacat
       61 cacctttttt ctgtcaggat ataaatttga caatgtcaaa aagataaaat aaaatttaat
      121 taaaattaac aagcaaaata ctggaggaaa aaatgaaaaa gagtgtaaat ataggtgtaa
      181 taggtagtat ggattttgaa aaagggcttt caataattaa agatgctggt tttgatgcag
      241 ttgaattaaa tttaagtggt gttttaagta tggaaagttc ggaagaagaa gtaaaaaaag
      301 taaaagaaaa agtgaattca aaaggacttg aaatagcaag tttacttgca ggtgctttct
      361 ggcaatttcc attgacaagt gataaccagc agaaaagaca gtcaggagaa aaacttcttt
      421 taaaaagtat agaaatatgt aaatatcttg aaacagatgc agttcttgtt gttcctggtg
      481 tagttggaac tgtatgggaa agtgaagaga gagttaaata tgatattgca tataaaagaa
      541 gtcaggaatc aataaaaaag tgcgttgaat tagcagaaaa aaataatatt tatatatgtg
      601 ttgaaaatgt gtggaataaa tttcttttaa gtcctcttga aatgaaaaat tttgttgagg
      661 aaattggttc tgaatatgtt ggtgtttatt ttgatgtagg gaatatttta actataggat
      721 atccagaaga ttggataaga atactcgggg aaaaaataaa aaggattcat cttaaggact
      781 tcaaattaag cgtaggaaat atcaatggtt tctgtaatct tcttgaagga gatgttaact
      841 ggaaagaagt tataagtgct ttaagggaaa taaaatatga tggttattta actgctgaat
      901 atggaccata taaatttttc cctgagacaa taatttataa tttatccctt gcaattgata
      961 aaattattgg agggtgaaat agttgacatc aaaagaaaga atttatgatg ttcttttggg
     1021 gagaattcct gatagagttc ctgtttctct ttataaaata gacccatttg atgaaaactc
     1081 attctgggca caacataaaa gtttttctaa attattagag acagcaagaa atttacaaga
     1141 tacatttttc ttttataagt taaagacagg gtttttcttt tcctctcctg aattacttga
     1201 aattaaagta gaagaatatc aggatactcc tatttcaaaa acaattaaaa ttactgttat
     1261 tactgaatta ggtcctttaa caagaattgc aagaacatca aatatgagta tgcaccaatg
     1321 ggttcaaaaa ccatggattg aaagcgaaaa agatatacat aaatttttat ctcttccata
     1381 ttctccttat acacctgata taagtgaatt ttataaatta caagagaaaa taggagataa
     1441 aggtataatt gtagttgctt tacctgaccc acttggagtt gttggttttc tttttgcccc
     1501 tggtgatttt tcaaaatttg ctatagatta ccctgacctt attcataaac ttcttgataa
     1561 aatttatgag agattaataa acatatatac tttcatatca aaaaatgttg aaaatgtgat
     1621 aataagaata agaggagcag aatatgcaac tccaccgata cttccagaag aatattttaa
     1681 tataaaaaag gcattttggg attttgttat tagatatgat accaatctta ttgaaattct
     1741 taaaaagggt aaaaaaaatt atatatgtta ccactggcat gaccatattg atgatttaat
     1801 tagttatgtt atgaaattgg ggatacatat aatagaacca atatcaaata cttttgagaa
     1861 accaaatttt attttaaaaa taagaaaaat agttggtgat gaagttgtga ttatgggtgg
     1921 agtagatgca attgatatgg agtttaaaac acctgaagaa attactcaat tggtaaaaga
     1981 aaccataatt cagggaggta gaaaaggaag atttatttta attccatccg atatacctga
     2041 atcagcccca ttaactcctg aacttgaaag aaattatatt acttttttac aggcagcaat
     2101 acagtatgga aaatatcctc tttaatagaa tttataattg gagcagttta gaagcattat
     2161 tataaaatat catttcttca agtttagaag atagttttaa tttctttaag ttttctattg
     2221 tttcaccctg gtctgcccat ggtgaatcag tcccaaaaag tatatattca ggtggatgat
     2281 ttaaaataat tctttttgcc ttgtcttcgt ctaaaaattg tagagaaaaa gatatttcca
     2341 tatagatttc ttttccaact ataaattttt caacttcatc ccacatttcc caggcaccaa
     2401 gatgagtagt tacaagtttt agagagggaa atgcattttt aatttttata attttttcag
     2461 ggtctgcttt tcttatcctc tcaaatgcaa aatcaaaacc agtatgtgaa acaagaatca
     2521 aatcaacttc ttcaattttc gcataaatat caaacatttt tttatcatct aaattgaaat
     2581 tctggtaata tggatgtaat tttattcctt taaaattatt ttttttaatt tcatcaagta
     2641 ttttttccca ctctttatca tctggatgaa aagaaaggaa tggtattatt ctttcatttc
     2701 taatttcttt tgaccagttt aaaattgagt tatattgaga tacctttgtc gcaattgaac
     2761 aaataacact tttatcaatt ttatatttat ccattgaact aagtagactt tttatttttc
     2821 catcatgtct tgcctttatc ccaccctctt tttcaagtaa aggtattgct ttttctgcaa
     2881 caacatctgg aaaagcatgg gtatgaaaat caataatttt actcattttt aaaaattttt
     2941 aaaatttcat ttgcaattat tgtatgtcct gtttgatttg gatgaactgg ttctggtgca
     3001 aatgtctcac tttctctata ttcaagatga tgttggaaaa ttctatgtaa gtttattaaa
     3061 tatgttttat atttattact catttcctga acaatttgaa tataattttc aatttctttt
     3121 aaaacagttc ctctaaaact atctgtttta tctattgtta tataaaatgg ttccattaaa
     3181 attattttac aatcaagttt ttcaactgtt ctttgcaaaa tattgttgta ccattttcta
     3241 aaactttcag gtatatattc ttccaataat tcattttttc ctaaaactct atgaaggtca
     3301 tttattccta ttaaaatagt taaaatatct ggcttattat aaataacatc atcttcccat
     3361 ctattatgaa ggtctaaaac tgtatttccc cctattcctt tatttattat ttcaaacttt
     3421 ttttctggaa aagttgaaat taaaaggtct ctaaaaatcc tgacataacc atttccataa
     3481 ggataaaatg catctcttct tccacaatct gtaatactat ccccaataca aagaatttta
     3541 tttacttccc cttcttttaa taatttctcc attttttctc cttttaaaat tttttgtttt
     3601 taaagtataa tacaaagaag aaaaatgtca aaaataaaat taatagcggt tgaccttgat
     3661 ggaaccttgc ttactgataa aagagaaatc catagaaaaa acaaatatta cttaaaaata
     3721 ttctcaaaaa aaggtggaat tgttgttctt tcttcaggga gaatgagtaa tagtgtttct
     3781 ccattttctg ataaattggg aattgattgt cctttaattg tttataatgg agcaatggca
     3841 agattaaaaa aaagtgaaaa cagaaaaata atatttcata aaccacttcc ttcaaaatat
     3901 tctgattata taattgacta ttgtcttaaa aataaattcc ttcttaattt ttacataaat
     3961 gataaactct attctcaaaa tgaccccggg ttaaaaaaat atgctcttat ttactcaact
     4021 caaactaatt caaaatatac attccttgat aaccttgcaa aactaaaagg aaaaagtcca
     4081 acaaaactta ttctcataac tgatagtaaa aataaagata ttgaaagaac aagggactat
     4141 caatataaat ttttcaaaag aattttcaaa gataaattaa aaattgtcag aacaaatcct
     4201 gaatatcttg aatttatgaa taaaaatgtt gataaatcag ttgccctaaa aaaaattgct
     4261 caatattata aagttaaaaa ggaagaaatt atttcatttg gagatggaga aaatgatatt
     4321 gaaatgatgt gtctttgtag attttctgtc tctccctcaa atgcaaaaga agatgtaaag
     4381 aaaattgcaa ccatttcttt aaaatattcc aataatgatg gatttatagg tgaattttta
     4441 aagatattag atttctaata aatattcctt aggaaaaata ttatcaagag aaagaataca
     4501 tgcagggtca aaaatttttt taattcttac catatctaat attcctcttt tcccatacat
     4561 aatttcaagg tatttatgtt ttaatttccc tattccgtgt tcagcaaaac cagttcctcc
     4621 aaaagaaact gcctttttta ctgcttcttc ataaattctc catgcttttt ctttttgttt
     4681 ttcatctttt ggaaaaagat taaaatgaag atggttttct ccaatatgcc caaagagtat
     4741 catatgaagg tcagtgtttt gttttataat ttctgtataa aaattataca tcaaaggaaa
     4801 tttatcttct ggaactgcgg catctactgc aacttttaca cttccgattt ttttaaaata
     4861 tgcattaaca ttatcaggta atctatgtct aaaatcaatt agtttttcat aattttgttt
     4921 atcttcccct atcattgtct caacaacttt tatttcctct gctatttcag cccatctttc
     4981 cattttttca atttctgaaa ctccttcaca ataaattgca caggtattgt caggaatttc
     5041 aggaaaatca ctttttaaaa actcaagtgc ttttgaatcg aaaaattcta aagaaaaaag
     5101 ttgtaaaaat tccttttcac tttttatttt ttttataatt tctggtatgt tttctttatt
     5161 gccaacaaat aaaactatta taaatctttc aggtaatttt tctattaaag aaacatcaat
     5221 ttctgtaatt attccaagtg tcccttctga accaattaaa aggtcaatcc catccattcc
     5281 cttttgtgag taatatccag cagaacattt tacatcagga gttctgtaag aaggcaaagg
     5341 tattttaaaa catccataat caataaagtt gtctttttca aaaacatccc ctcttttaat
     5401 ttcaaatatt tctccattac ttcttatcat tcttattttt ttaacatatt tccttgtggg
     5461 accaaatctg aaactatatt cccctgatgc attggttgca atattacctc ctataaaagc
     5521 agttctttct gtcggaaatg gtgggtaaaa aagagaatat ttatctaatt cctttaaaaa
     5581 ttcatcaaca ataactcctg cagaaagaca tgctgtttta ttttctttat ttataaattt
     5641 aattttatta aatttttcaa gagaaattat ccctccctca acaggtattc ttccacctac
     5701 tgttcctgtt ccaccaccag aaattgttaa tggaaattta tttttactgc tttcttttaa
     5761 aatagataca atttcatctt cattttcagg tataaaaaca acttctgctt ttccaccttt
     5821 aaaatttgaa gagtcctcta aataagaaat tatttcctct ctgtcttttt ttactatcat
     5881 tttttcttcc tgtgttaaaa tttggtactg ccaaaattgg ttatagcttt tataatgctt
     5941 ttatttatct tttaatgctt ttatatgtct ttatatccct gccctttttt atctattttt
     6001 tgacactacc tttaaaatgt tcaagtttta cactcataat ctcctgtcaa aattgaaaat
     6061 gggatttaat tatatgaatt ttttcctcac aactgccaat ctctttctgc ccctttatag
     6121 cacttacttt caataaatat tctcctgctg ttaaattcac cgggtttatc ccaaatttta
     6181 catagttgtt tccatctgct tttatctctg tcaaataatt attctcttta ttacttattt
     6241 caaatttgaa tttcacatct tttaattccc cttctcctaa atttatcctt atattacctt
     6301 caatttgttt gtctgttgta taaacctgag caagttttat tttcatctct aatacttacg
     6361 gtaaatatat cccctttaca taatatgttg aataatctct cccaatttct acatttgcta
     6421 tttgttaaaa ttctttcccc ttcccaaatg atattagcca aattccttcg gctgatgaaa
     6481 tcctcctgtc gttactttcc attgacttag ttcatttatc tcccagttgg tatgacaaaa
     6541 attcgctccc caaatacacc ccttcggcac actccttaat ccaaaatcac taaatgccta
     6601 aattccgcca taattaggca ctaagtaagt ttattatatc atatattcat cacagcaaca
     6661 gggtttttta acaaaggtag atgctatact taatgcctat atgaacatta taagaagaaa
     6721 atatgtcttt atccccattt gagtttatac atacagtaaa aacatttccc ctcaccttaa
     6781 atccctcagg cgtagtccac atgctatccc ttacccacat cttctacaat cctcttggat
     6841 aaagagattc cctttgct
//
LOCUS       DAOOVW010000064         6822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_6577_length_6822_cov_9.261859, whole genome shotgun sequence.
ACCESSION   DAOOVW010000064 DAOOVW010000000
VERSION     DAOOVW010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6822
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6577_length_6822_cov_9.261859"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            600..1265
                     /locus_tag="PLW95_07505"
     CDS             600..1265
                     /locus_tag="PLW95_07505"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HOV22500.1"
                     /translation="MEILNDFPLSVDKELIMQKLKIKKGTEDEKKFEQLLNEGMKIGN
                     PKVVYIEGFIDELGEKTVKINGIIFESKLLRKNLEKVGRVFPFIATSGMEMENKKFGN
                     DDFLEKYWWDIIKEQFLFIARDHLIEYLKKNFSLGNVSIMSPGSGELGLWRIEEQKKL
                     FSLFGDTEKLLGVKLTDSNLMIPNKSVSGIIFSTEKNFNTCKLCSRKNCPHRKAEFEK
                     VFK"
     gene            complement(1269..2384)
                     /locus_tag="PLW95_07510"
     CDS             complement(1269..2384)
                     /locus_tag="PLW95_07510"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOV22501.1"
                     /translation="MIEIAKKSDYEKILRFLESAYGHFFNFFPLTYPNEWEEENFDYE
                     NTLIIKEEGQIVSLVKVFPLPMVFEGIEVKFAGIGSVSTAYSHRGKGYMSELLNECFE
                     KMKKENYPLSILWGDRHRYQNFGYEVAGKKVYITITSRGIAKNKIESDKKTERFLGDR
                     KILNKIISAYNLHPYRKIRTEEEMFEKVLKKFLPATYYLEGQNKFAYVSISREGGILE
                     FGGDEELILKILKYLEERFSYSSFSLFYPDFEYIPDLIFSTASSWQICPDGMIKIIDL
                     FQTLKIFSKKFLSIPENFEITFSTENQSAILRKEKGDIIIKNGKGKNEIFLNEIDMVK
                     LFFGTDFWKPKGGSKEILNILKSVLPINVFIWSLDHI"
     gene            complement(2443..3762)
                     /locus_tag="PLW95_07515"
     CDS             complement(2443..3762)
                     /locus_tag="PLW95_07515"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HOV22502.1"
                     /translation="MKNKLWLIIMFIFTTAVFCEANFDYGLIFRLREEYLKNAWDFNN
                     QSNVFQNDNYFRIRSSFWMKYNFGENISLYTRLTGEPDIYLHSKGTLRNMAGNYIGDN
                     EIVIDNLYLDVKNVFNLPVDLRIGRQDFLPPYGEGFLIINGTPQDGSRTLYFNAIKTT
                     YHFNENNSLDLIFLYNRDEDKYLPVINDLETKLINSDEKGVILYGNFKPTEKLSLQPY
                     YIYKSEDAYTTQNGTNIPKLELNTIGVRHVYSFNPWQLRGELSYEFGEYEDDDNRNAV
                     GGYVYLTRFFKDKKFSPSLDFGFGYLSGDNDKTKGTEGWDPLFSKFPWISELYLYAYA
                     IEAGEPAYWTNTQLWRTALTLNLSEKTDLLIAYNYLRANENLDGTAPFFGDGKERGHL
                     QQLILEHNFNKNVSGHLWCEYFIPGDFYSSNNQDPALFFRWEMIFKF"
     gene            complement(4212..5093)
                     /locus_tag="PLW95_07520"
     CDS             complement(4212..5093)
                     /locus_tag="PLW95_07520"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOV22503.1"
                     /translation="MRMLIRILIDAVIIIFVLTIIFGWRAIMPARAPRYLIPSDLGIP
                     YEKVNFQTDDGKKLTGWLIHSPKSKSVIICLHGYPSSKSDILPVVSYLYPDFSLLLFD
                     FRAHGESKGKVVYFGLKEYLDVKAAIDFLKNDKRTKNLKIGVWGYSLGASVGIISASK
                     YQEIKCLVSDSAFANFPEMITHYYKKFGPLKYAFSYLSNLLGKIILKDDFMENSPENY
                     IDKVKCPVLIIHSREDDFVPFSHAEILYNKIKGEKELFVLEGSHVDFDARYNQEYRDK
                     VKEFFCKYLNEKVVSKK"
     gene            complement(5077..5613)
                     /locus_tag="PLW95_07525"
     CDS             complement(5077..5613)
                     /locus_tag="PLW95_07525"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HOV22504.1"
                     /translation="MGKIKNPIPVKIFCGFIYKDAEIILKVEEKLKERWGKIDFVSYP
                     LPFNFTDYYSEEMGKNLMRKFISFSKIILPEKLWEWKIFTNKVEEEFLENGKRKVNID
                     PGYLDLSKIVLLSTKDFYHRIYLGNGIYAEVTMYFSKGKYQFFPWTYPDYRTENYIRF
                     FENIRELYSKQLKNENVN"
     gene            5684..>6822
                     /locus_tag="PLW95_07530"
     CDS             5684..>6822
                     /locus_tag="PLW95_07530"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOV22505.1"
                     /translation="MKKKIYLVFIVLFLFFVNSFAQQGKEKLPLITENKEVKNIILFI
                     GDGMGLSQIAATHIKTSGAEGILNMECMPITGLLYTYSANSLITDSAAAGTALATGYR
                     TNNGIISMSPDGKVLYTILEAARDKGASTGIVVTSPITDATPACFVCHVMARTKETEI
                     ASQYLKSKVNVLLGGGRQYFIPQSSSGSKRDDERDLISEAKKIGYSFVKTKEEMMNVK
                     TNYLLGLFEMGSLSTKPEPTLAEMTQKAIEILSQNKKGFFLMVEGSQIDKWAHVNNID
                     NVIQQTALFDESIKVGIDFAMKDKQTLVIVLADHETGGMSIIRGNLNGEDMQVRWASP
                     SHTPIPVILFAFGPMAEKFTGVNHHTYIPKTISEILGIKNFPKII"
BASE COUNT         2355 a         1091 c         1019 g         2357 t
ORIGIN      
        1 tccatcccga ccttcccttt ataagggaag gagagatgct tccccctttg aaaaaggggg
       61 attgaggggg atttatttcc ttctccccgt tggggagaag gtggggatga ggggggattt
      121 gatactttct cctctttaac aaaccgactg aagttgctta ccttgtgcct cagagttggt
      181 gcgttatcgt cttccagata gcacaacagg gcttagggga gatttgggaa attacaggaa
      241 atccctcaca tccttctccc ctccgccact gatacgttgg cgggctatcg cactaagaca
      301 ttgacggaca tgctttttta aaggaaggga aaaaagtttt ccccctcacc catacccctc
      361 gggcgtatga catacgggcg agcctctccc cactggggag agggaagaaa gaaaaaaagg
      421 gagaggaaga gaagcaaaaa gaggggggag ggatataaag acatataaaa accttaaaag
      481 acaaagaaag gcattataaa agctatagcc aattttggca gaactggcgg agaggcgagg
      541 gggttattat gaaagttatt tgtcaatttt ggcaatactt gaaagccaag aggagaaaaa
      601 tggaaatttt gaatgacttt cctttatcag tggataaaga actaattatg caaaaactaa
      661 aaataaaaaa agggacagaa gatgaaaaga agtttgaaca attactaaat gaagggatga
      721 aaatagggaa tccaaaagtt gtttatatag aaggttttat tgatgaattg ggagaaaaga
      781 cagttaaaat aaatggtatt atttttgaaa gcaagttatt gagaaaaaat ctcgaaaaag
      841 ttggcagggt ttttcctttt attgcaacat caggaatgga aatggagaat aaaaaatttg
      901 gaaatgatga ttttttagaa aaatattggt gggacataat aaaagagcaa tttttattta
      961 ttgcaagaga ccatcttatt gaatacttaa agaaaaattt ttcacttggt aatgtctcaa
     1021 ttatgtcacc tggtagtgga gaacttggat tatggagaat tgaagagcag aaaaaactct
     1081 tttccctttt tggtgataca gaaaaacttc ttggggtaaa attaactgat tcaaatttaa
     1141 tgataccaaa taagtccgtt tcaggaatta ttttttcaac tgaaaaaaat tttaatacct
     1201 gtaagttatg cagtagaaaa aattgtccac atagaaaagc agaatttgag aaggttttta
     1261 agtaaatttt aaatatggtc taatgaccat ataaatacat ttattggtaa aacacttttt
     1321 aagatattta aaatttcttt gctccctcct tttggtttcc agaaatcagt cccaaaaaat
     1381 aattttacca tatcaatttc atttaagaaa atttcgtttt tccctttccc atttttaatt
     1441 ataatatctc ctttttcttt tcttaaaatt gctgattggt tttctgttga aaaagtaatt
     1501 tcaaaatttt caggtattga tagaaatttt tttgagaaaa tttttagtgt ttggaataaa
     1561 tcaataattt ttatcatacc atctggacat atttgccaag aggaagcagt agagaaaatt
     1621 aaatcaggaa tatattcaaa atcaggataa aaaagagaaa aagaagaata actaaacctt
     1681 tcttcaagat attttaaaat ttttaaaatt aattcttcat ctccaccaaa ttctaaaatc
     1741 cctccctctc ttgaaattga aacatatgca aatttatttt ggccttcaag ataataagta
     1801 gcaggtaaaa attttttcag gactttttca aacatttctt cttctgttct tatctttctg
     1861 tatggatgta aattataggc agaaataatt ttgtttagaa tttttctatc tcctaaaaat
     1921 ctttctgtct ttttatcact ttctatttta ttttttgcaa tgcctcttga agtaattgtt
     1981 atatatacct ttttaccagc aacttcatat ccaaaatttt gatatctatg tctatctccc
     2041 cataaaatag ataaaggata attttctttt ttcatttttt caaaacactc attaagcaat
     2101 tcactcatat atccttttcc tctatgggaa taagcagttg atacagaacc aattccagca
     2161 aatttcactt caattccttc aaataccatt ggtaaaggga aaacttttac aagagaaaca
     2221 atttgtcctt cttcctttat gattaaggta ttttcataat caaaattttc ttcttcccat
     2281 tcattagggt aagtcaaagg gaagaaatta aaaaaatgtc catatgcact ttcaagaaat
     2341 ctcaaaattt tttcataatc actttttttt gctatttcaa tcacttttcc tccttctgtt
     2401 aacttcgtaa gttaacaagt tattgaaaat caataactta cattaaaatt taaatatcat
     2461 ctcccatcta aaaaataatg cagggtcctg attattagat gaataaaaat cacctggaat
     2521 aaaatactca caccataggt gcccacttac atttttatta aaattatgtt ctaaaattaa
     2581 ttgctgtaaa tgtcctctct ctttcccatc accaaaaaat ggagcagtac catctaaatt
     2641 ttcatttgct cttaaataat tgtaagcaat caaaaggtct gttttttctg aaagattaag
     2701 agtaagtgca gttctccata attgagtatt tgtccagtaa gccggctcac ctgcttcaat
     2761 agcataagca taaagataaa gttcacttat ccatgggaac tttgaaaata aagggtccca
     2821 tccttctgtt ccttttgttt tatcattatc tcctgacaaa tacccaaaac caaaatcaag
     2881 agatggagag aattttttat ctttgaaaaa tcttgttaaa taaacatatc caccaactgc
     2941 atttctatta tcatcatctt catattcacc aaattcataa gatagttctc cccttaattg
     3001 ccacgggtta aaagaataga catgtcttac tccaattgta ttaagttcta attttggtat
     3061 atttgttcca ttttgtgttg tatatgcatc ttcgctttta taaatgtaat atggttgaag
     3121 tgaaagtttt tcagttggtt taaaattccc ataaagaata accccttttt catcagaatt
     3181 aattaatttt gtctctaaat catttattac tggcagatat ttgtcttcat ctctattata
     3241 aagaaaaatc aaatcgagag aattgttttc attgaaatgg tatgttgttt ttattgcatt
     3301 aaaataaaga gttcttgaac cgtcttgagg agtgccatta ataattaaaa aaccctctcc
     3361 ataaggaggc aaaaagtcct gtctaccaat tcttaaatca acaggtaaat taaaaacatt
     3421 tttaacatca agatataaat tatcaataac aatttcatta tctcctatat aatttcctgc
     3481 catatttctt aatgtccctt ttgaatgtag atatatgtct ggttctcctg ttaaccttgt
     3541 gtataaagat atattttcac caaaattata tttcatccag aaggaacttc ttattctaaa
     3601 ataattgtca ttttgaaaaa cattactttg attattaaaa tcccaggcat ttttaagata
     3661 ctcttctctt aacctgaaaa ttaaaccata atcaaaattg gcctcacaaa aaacagcagt
     3721 tgtaaaaata aacataataa tcagccataa tttatttttc attttttctc cttgtttttc
     3781 tcattgattc ttaaaaattt attggcatta cttctaaatc catccctacc cttcctttaa
     3841 aaaaggaagg gaattaaggt gaaaaactat ctgcttttgt cttatttccc ccttttagaa
     3901 agggggattt agaaatccat ccactcaggc gtagtttatc gctgagtctc tccccgctct
     3961 gcggggagag ggaaatcccc ccaattcccc ccttttgtaa accgactaaa gttgcttacc
     4021 ttgtttctaa gagttggtgc gttaaataga aatccatccc ggccttcctt taataaagga
     4081 aggagtaaca ctctcccctt taacaaaccg attaaagttg cttaccttgc ttctcagagt
     4141 tggtgcgtca tcgtctccca gatagcacaa cgggagttag aggggatttt aatttttcat
     4201 ctttctttca tctatttttt tgacactacc ttctcattta agtatttaca aaaaaattct
     4261 tttaccttat ctctatattc ttgattatat cttgcgtcaa aatcaacatg acttccttct
     4321 aaaacaaata attccttttc cccctttatt ttattatata atatttcagc atgagaaaaa
     4381 gggacaaaat catcttctct tgaatgaata attaaaacag gacatttgac tttatcaata
     4441 taattttccg ggctattttc cataaagtca tcttttaaaa taattttacc aagtaaattt
     4501 gaaagataag agaaagcata ttttaaagga ccaaattttt tatagtaatg agttatcatt
     4561 tcaggaaaat ttgcaaaagc ggagtcagaa acaagacatt ttatttcctg atattttgag
     4621 gcagatataa ttccaacaga agcacctaag gaatatcccc agacaccaat ttttaaattt
     4681 tttgttcttt tatcattttt taaaaagtca attgctgctt ttacatcaag atattctttt
     4741 aatccaaaat aaacaacctt tcctttactt tcaccgtgtg ctctaaaatc aaaaagtaaa
     4801 agtgaaaaat caggatagag atatgaaaca acaggtaaaa tatctgattt acttgatgga
     4861 taaccatgaa gacaaattat aactgattta ctttttggtg aatgaattaa ccatccagtt
     4921 aattttttcc catcatctgt ttgaaaattt actttttcat atgggattcc caaatcagaa
     4981 gggatgagat atcttggtgc tctcgctggc ataattgctc gccagccaaa aataattgtt
     5041 aaaacaaaga taattattac tgcatctatt aaaattctaa ttaacattct catttttaag
     5101 ttgctttgag tatagttctc ttatattttc aaaaaatctt atataatttt ctgttctata
     5161 atcaggatat gtccatggga aaaactggta ttttccttta ctgaaataca ttgtaacttc
     5221 tgcataaatc ccatttccta aataaattct atgataaaaa tcctttgttg ataaaagaac
     5281 aatttttgat aagtcaagat atcctgggtc aatatttact ttccttttcc cattttctaa
     5341 aaattcttct tcaactttgt tggtaaaaat tttccattcc cataattttt caggaagaat
     5401 aatttttgaa aaggatataa attttctcat taaatttttt cccatttctt cactataata
     5461 gtcagtgaaa ttaaagggca aaggatatga tacaaaatca atttttcccc atctttcttt
     5521 aagtttttcc tcaactttta aaattatttc tgcatcctta tagataaatc cacagaaaat
     5581 ttttacaggt attggatttt ttatttttcc cattttttta ttttaccata tttaaaaaaa
     5641 tcaaaaattg actgtataat attttgagac gaggagagtt attatgaaaa agaaaattta
     5701 tttggtattt attgttttat ttttgttttt tgtaaatagt tttgctcaac agggaaaaga
     5761 aaaattacct ttaattacag aaaacaaaga agtaaaaaat ataatacttt ttattggtga
     5821 tggtatggga ttatctcaaa ttgcagcaac acatataaaa acatcaggag cagaaggaat
     5881 ccttaatatg gagtgtatgc caataacagg tcttctttac acttattctg ctaattctct
     5941 tataacagat tccgcagcag caggtacagc acttgcaact ggttatagaa ctaacaatgg
     6001 cattatttca atgtctcctg atggtaaggt tctttatact atacttgaag cagcaagaga
     6061 taagggggcg tcaacaggga tagttgttac aagtcctatt actgatgcta cacctgcttg
     6121 ttttgtctgt catgtaatgg caagaactaa agaaacagaa attgcatctc aatatctaaa
     6181 aagtaaagta aatgtattac ttggtggtgg taggcagtat tttattcctc aatcttcttc
     6241 tggtagcaaa agagatgatg aaagagacct gatatcggaa gcaaaaaaaa taggttattc
     6301 ttttgttaaa acaaaagagg aaatgatgaa tgttaaaaca aattacctct tagggctatt
     6361 tgaaatgggt tcattatcca ctaaaccaga gccaactttg gcagaaatga cacaaaaagc
     6421 aattgaaata ttaagtcaaa ataaaaaggg atttttccta atggtagaag ggtcacaaat
     6481 tgataaatgg gcacatgtca ataatattga taatgttatt caacaaacag cactttttga
     6541 tgaaagtata aaagttggga ttgattttgc aatgaaggat aaacaaacat tagttattgt
     6601 tcttgctgac catgaaacag gaggaatgag tattataaga ggaaatttaa atggtgaaga
     6661 tatgcaggtt agatgggcat ctccaagtca tactcctatt cctgtgatat tatttgcatt
     6721 tggtccaatg gctgaaaaat ttacaggagt taaccatcac acttatatcc ctaaaacaat
     6781 ttctgaaata cttggtataa aaaattttcc taaaataatt ga
//
LOCUS       DAOOVW010000065         6771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_6619_length_6771_cov_7.840530, whole genome shotgun sequence.
ACCESSION   DAOOVW010000065 DAOOVW010000000
VERSION     DAOOVW010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6771
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6619_length_6771_cov_7.840530"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(777..1646)
                     /locus_tag="PLW95_07535"
     CDS             complement(777..1646)
                     /locus_tag="PLW95_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HOV22506.1"
                     /translation="MSIKYAVPVKIDIKSHPELNETWVQNIIAEDPSIIGLGNLVLKD
                     KERIQPRAGRLDLLLQNVDATKRYEVEIQLGKTDENHIIRTIEYWDIERKRFPQYEHC
                     AVIIAEDITSRFLNVINLFNGAIPLVAIQMNAIQVGDTISLVFTTVLDQLKLGFVDED
                     EEVYEITDRNYWEQRAKEQTVAMADKILELINSIWPGFELKYNKCYIGLAKDGQPNNF
                     VIMRPQKNIMRVEFKIPKSDDIENKIESAGLDMLDYDSRWERYRIKLNKPDIEKNKDF
                     IIELLKAAYENNL"
     gene            complement(1932..2771)
                     /locus_tag="PLW95_07540"
     CDS             complement(1932..2771)
                     /locus_tag="PLW95_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22507.1"
                     /translation="MSIENFKKWIEAGHTIYDIFEGRYNVYPICRNWVEKWYENDKFS
                     ISQDDIERLNEIRKNFYYAAFEIAPQLQQRMDDAFQQLIIFLDENRCQYNIGLGVAPY
                     LFLWNFRRFKEYFEDERFNLSEYFQEIGYFFQDKRDIIKEYRQKKIYREEINENEIKQ
                     ILNDLNDCLKKCGRNNNEPVGVAKSLHIFAPYYFPLIDNSIAKATGLIKSQRDGLSLE
                     KYFNWIRYMKKWVETYNIAEIQEIEERHNLSILKLIDEGLYISCSIHLGSRIKILGFE
                     IDN"
     gene            complement(2901..4424)
                     /locus_tag="PLW95_07545"
     CDS             complement(2901..4424)
                     /locus_tag="PLW95_07545"
                     /inference="COORDINATES: protein motif:HMM:NF021593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HOV22508.1"
                     /translation="MEFNLKKCRDCFKEAYYEVYGKQINIEPLEQFFRNSLSQQTLTY
                     ETLISVIDKKYWEFGKHLNPPPKTQIEPFLEKINSLLNEKLPENEEFVIKELVMIFHD
                     LQFTSLILRFAHPEFYGIYSTPVIAITRVETTRAIDQYMKYLKRLREWKETYGFERVA
                     DVDHAAWSLAEKEDMGKCVEKKCENYFLYKRDPKIISDIAFSLYDRKKTEEAIEKLNN
                     AKEICNDKEPIFQTLMMIYEEIKNYSEMINEGIELINWYKEKKNYYKIPDVCEQIYKY
                     EKIVEILEDEIEAYKHIGDKNKQIEKLITLAELYKQDGQIKKAMEVYNKIILIAPEQK
                     EIISELKEKLEEFAEEQKSIGKGIKICLKCQKLAEEIDCIYILLPKEKWNECELCTIW
                     AKKYDQYKELSGRIITIVGGKESLKERYKEEIKRLGAKDCLFHNAIDNINEISSLVKK
                     GDYGIIITSIASHAGTNKAKSEFEKEKKIFIRLHQTGIDSLVETVVNNLIPRIRNIQ"
     gene            4679..>6771
                     /locus_tag="PLW95_07550"
     CDS             4679..>6771
                     /locus_tag="PLW95_07550"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012992322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOV22509.1"
                     /translation="MTKEEKFYKALQDVFIGAKIEGTGGFINLMRIKSNYYRKIEDIL
                     KKDIESALKNYPKFRDELFDKLYSFFNRYFTESGSIYFNQTPFHNNVYEKVYTDEKDV
                     VLFWKTQMLYYVKTDRIFRSLPFEFDGLKFFFDASTIENKKSNEKRSVVFELDKIRED
                     NTIVFSVSYSERGTKTKQDEILKNIKKKGIAITEEQLERAFRVFEKQSEVDFFINKNA
                     KAFLQEQFKLWSYQYFWEGSQEWGADRVNQLQILKDIAFKIIDFIGQFEDELVKIWNK
                     PKFVKNSNYVITLDRIKDENLIEKIKKHVGIKEQIKEWQEIGIVDKNFKIKEIFEIDL
                     TGKHLSKKYEHLPIDTKYFKDLEIEILSQFDDLDKSLDGWLIKSENYQVLNTILPKFK
                     EKVQTIYIDPPFNLNSSDQFLYRTNYKDANWATLLENRLRIAKDWLNEKGSIFVRCDY
                     NGNWIVRCLMDDIFGRENFRNEIFVKRGYVPKGLTNQYLTGIDSIFFYAKDVNKMAFK
                     GAKRKIREEDRQWISLDMPGQRRTYELQVRYFFGKPWLPPKGQHWGLSQQKITEYEKL
                     EWIRINPGRKYIDTQGNTVKGMPEYLKEPELLLDTNWTDIKSYETHNTFFATENSEIL
                     LKRVIEGMEYDKQLIIMDFFLGSGTTTAVAHKLGRKWIGVEMGEHFYSVVLPRMKKVL
                     AYDKSGISKEVKEYQG"
BASE COUNT         2331 a         1040 c          932 g         2468 t
ORIGIN      
        1 accttgcaat tattgctgaa cgaaaaaaag aaaaagcaat aaaacttgac aactatctta
       61 caagaatgag agagaatgga tatttataaa atataggtta aaacttctat agaaaaagat
      121 ttaagaaaaa ttccccacca atatataggt aaaattattc aagaaataga aaatctctct
      181 acaaatccgt accctactgg aacagtcaaa cttacagcaa ctgaaaatac ttatagattt
      241 agagtgggaa attatagagt gatatatcaa gtaataaaaa gcaaaagaac aattatcgtt
      301 caccatataa gacacagaaa agaagcatat aggtaaattt ctctcatcca tcctgtctat
      361 tgtatttctt gatttttata cacccccatt tcaatgtccg aatgtccgaa aaattaaaga
      421 aacctatatc agataaggaa tttggcatct gacatcagat tttcaatgtc tgtacctatg
      481 tctaaatgtc agaaaatagt ttttatcttc cccttaaaaa tgcctttctt ttttaaaaaa
      541 ggttagttca gacgatattt tttgaaagaa aaattaaaag atggttttta ctgcctaaag
      601 ggttttcttt tttaaaatac ctatagaaaa cgggttagga agacaattag cctattttgg
      661 gtatacatct caaatagtgt atttattata tctttatact ataataaatg aaaaatctat
      721 aattgaaatt tagagaattt ttggaaagta aaagattaat aagaatatcg gctgttttat
      781 aagttatttt catatgctgc ttttaatagt tcaataataa aatccttgtt tttctcaata
      841 tctggcttgt tcaattttat tctgtacctt tcccacctgc tatcatagtc cagcatatca
      901 agtccggcag attctatttt attttctatg tcatcggatt tcggaatttt gaattcaact
      961 cgcataatgt ttttctgtgg acgcattatc acaaaattat taggttgtcc gtcttttgca
     1021 aggccgatat agcatttatt atattttagt tcaaaacctg gccagatact gttaatcaac
     1081 tcaagaattt tatcagccat cgcaactgtc tgttcttttg ctctctgttc ccaataattt
     1141 ctgtcggtta tttcataaac ttcctcatct tcatcaacaa aaccaagttt taactggtca
     1201 agcactgttg taaatacaag agaaatagta tcaccaacct ggattgcatt catttgaatt
     1261 gcaactaggg ggatagcacc attaaaaaga tttataacat tcaggaatct actggtaatg
     1321 tcctcagcaa tgataacagc gcagtgttca tattggggaa atctttttct ttctatatcc
     1381 cagtattcta tggttctgat aatatgattt tcatcggttt tacccagttg aatttctact
     1441 tcgtatcttt ttgtagcatc tacattctgt aagagtaaat caagtcttcc ggcacgaggt
     1501 tgaattcttt ctttgtcttt aagcacaagg ttccctaatc ctattatact tggatcctca
     1561 gctataatat tctgaaccca cgtttcattc aattccggat ggctcttgat atcaatcttt
     1621 accgggacag catattttat agacatttta cctccatttt gataataatt tctgataact
     1681 acactgtaaa gtatatcaca aatatttaaa ttttactata tatcaagtgg ttatattttt
     1741 ctaaattaag caaattttca cggcgacatg aaaaatttgc cactattcat aatcccctat
     1801 aaagtaagaa taatttttcc taattcttct acaaatctct tgtttataac aactttattt
     1861 ttctctcgaa tgacttcact attcaacaac tgataatgct tcttagagtt ttattttcat
     1921 attcaatctt cttaattatc tatttcaaaa cctaatattt ttatccttga acctaaatgg
     1981 atagaacagc ttatatacaa tccttcatca attaatttca aaattgataa attgtgcctt
     2041 tcttctatct cttgtatttc tgctatatta tatgtttcaa cccatttttt catatacctt
     2101 atccaattaa aatatttttc taatgataaa ccatctcttt gggatttaat taatcctgtt
     2161 gcttttgcaa ttgaattatc tatcaatggg aaataatatg gagcaaatat gtgtagtgat
     2221 ttagcaacac cgactggctc attattattt cttccacact ttttgaggca atcattaaga
     2281 tcattaagta tttgttttat ctcattttcg tttatttcct cacgatatat cttcttttgt
     2341 ctatattctt ttataatatc tcttttatcc tgaaaaaagt atccaatttc ttgaaaatac
     2401 tcagataaat taaacctttc gtcttcaaaa tattctttaa atcgtcgaaa attccacaaa
     2461 aatagataag gagcaactcc taaacctata ttgtactgac atctattctc atctaaaaaa
     2521 attattagtt gttgaaaagc atcgtccatt ctttgttgaa gttgaggagc aatttcaaaa
     2581 gcagcatagt aaaaattttt tcttatttca tttaatctct caatatcatc ttgtgaaatg
     2641 ctgaatttat cattttcata ccatttttct acccaattcc tacatattgg ataaacatta
     2701 tatctacctt caaaaatatc ataaattgta tgtcccgctt caatccattt tttgaaattt
     2761 tcaatagaca taattacacc tcctacaaaa aaatttaaat ttttttatat tacatttttc
     2821 taactatagg aatcctctct gagttaaaaa tatttagata aaaaaaacaa agccgtgaag
     2881 caatctctcc gaccttttta ttattgtata tttcttattc ggggaattaa attatttaca
     2941 actgtttcta ctaaactatc tattcctgtt tgatgaagtc ttataaaaat tttcttttcc
     3001 ttctcaaact cactttttgc tttattagtt ccagcgtggc ttgcaatact cgtaataatt
     3061 attccataat ctcccttttt cactaaacta cttatttcat ttatattatc aattgcatta
     3121 tgaaataaac aatcttttgc acctaatctt tttatttctt ctttatatct ttcttttaaa
     3181 ctttctttac caccaacaat agtaattatt ctcccagata gttctttata ttggtcatat
     3241 ttctttgccc aaattgtaca aagttcacac tcgttccatt tctcttttgg aagaagaata
     3301 taaatacagt caatctcctc ggctaatttt tgacacttaa gacatatttt aattcctttc
     3361 ccaatgcttt tttgttcttc tgcaaattcc tctaattttt cttttaattc tgatattatt
     3421 tctttctgtt cgggcgcaat taagataatt ttattataaa cctccattgc tttttttatt
     3481 tgtccatctt gcttatacaa ttctgctaag gttattaatt tttctatttg tttgtttttg
     3541 tctcctatat gtttgtatgc ttcaatctca tcttctaaaa tttctactat tttctcgtat
     3601 ttatatattt gctcacagac atcaggaatt ttatagtaat tttttttctc tttataccaa
     3661 ttaattaatt caataccctc atttatcatt tcactgtaat tttttatttc ttcataaatc
     3721 atcataagtg tttgaaatat tggctcttta tcattacaaa tttcttttgc gttgtttaat
     3781 ttttctattg cttcttctgt tttttttcta tcatatagag aaaaagcaat atcagatatt
     3841 attttggggt cccttttata aagaaaatag ttttcacatt ttttttctac acattttccc
     3901 atatcttctt tttctgcaag agaccaagca gcatggtcaa catcggcaac tctttcaaat
     3961 ccatatgttt ccttccattc tcttaatctt tttaaatatt tcatatattg gtcaatagca
     4021 cgagttgtct ctactcttgt tatggctatt acaggggtac tataaatacc ataaaattct
     4081 ggatgagcaa atcttaatat taaagaagta aattgcaggt catgaaaaat catcactaac
     4141 tcttttatta caaattcttc attttcaggc aatttttcat ttaataaaga attaattttc
     4201 tcaagaaaag gttctatttg tgtttttggt ggaggattta gatgttttcc aaattcccaa
     4261 tattttttat caattacgga tattagtgtt tcatatgtca atgtttgctg agaaagagaa
     4321 tttctgaaaa attgttctaa tggttcaata tttatctgtt ttccatatac ttcatagtat
     4381 gcttctttaa aacaatcacg acattttttt aaattaaatt ccatttcttt attttaacaa
     4441 aaatttaata aaagaggaat atttctatat aagtttgtag atgataccat tttactctat
     4501 taaccagtat gaataaatgt tatattgcat tggctctttt tctttgtgaa attttaaatt
     4561 tgattggtaa aaatgtaaat gttaaaatat aaaagtagaa atagaattat aatttgttgt
     4621 aaaaaattac aaaagagagt ggtgagttat tttattaaat ataaccaaat aagtatatat
     4681 gacaaaagaa gaaaagtttt acaaagcatt gcaagatgtt tttatcggtg ccaaaattga
     4741 gggaacaggt ggttttatca acctgatgag aataaaatca aattattacc gcaagataga
     4801 agatatcctc aagaaagata ttgaatcggc tctaaaaaac tatccaaaat ttagagatga
     4861 actttttgat aaactttact ccttttttaa cagatatttt actgaaagtg gttcaattta
     4921 ttttaaccaa actccttttc ataacaatgt ttatgaaaaa gtttacacag atgaaaaaga
     4981 tgttgtttta ttctggaaaa cgcagatgct ttactatgtc aaaacagacc gaattttcag
     5041 gagtttgcct tttgaatttg atggtcttaa gtttttcttt gatgcttcaa caatagaaaa
     5101 caaaaaaagc aatgaaaaac gctcagtagt ttttgaatta gacaaaattc gtgaagacaa
     5161 cacaattgtt ttcagtgttt cttattctga aagagggaca aaaacaaaac aagacgagat
     5221 attaaaaaat ataaagaaaa aaggcatagc gattacggaa gagcaattgg aacgtgcttt
     5281 tagagttttt gaaaaacaga gtgaagtaga tttttttata aacaaaaatg ccaaagcatt
     5341 tctgcaagag cagtttaaac tctggagtta tcaatatttt tgggaagggt ctcaagagtg
     5401 gggtgcggat agagttaatc aactgcaaat tttaaaagat atcgccttca aaattattga
     5461 ttttatcggg cagtttgaag atgaactggt aaaaatatgg aacaagccaa agtttgttaa
     5521 aaactccaac tatgtgatta cactggatag aataaaagac gaaaacttga ttgaaaaaat
     5581 caagaagcac gtgggcatta aagaacaaat aaaagaatgg caagagatag gaatagttga
     5641 taagaatttt aagataaaag aaatttttga aatagattta acagggaaac atctctcaaa
     5701 aaaatacgaa cacttaccta ttgatacaaa atatttcaaa gatttagaaa ttgaaatttt
     5761 aagtcagttt gacgacttag ataaatcatt agacggttgg ttgataaaat ccgaaaacta
     5821 tcaagtatta aacacaattc tgccgaaatt caaagaaaaa gtgcagacga tatatattga
     5881 tccgccattt aatttaaatt catcagacca atttttatat cgcactaatt acaaagatgc
     5941 gaattgggca acgcttcttg aaaatcgttt gcgaatagca aaagattggt tgaatgaaaa
     6001 aggaagtatt tttgttagat gtgattataa tggcaactgg attgtaagat gtttgatgga
     6061 tgatattttt ggaagagaaa attttagaaa tgagattttt gttaaaagag gatatgtgcc
     6121 aaaaggatta accaaccaat atttaacagg tattgattca atattcttct acgcaaaaga
     6181 tgttaacaaa atggctttta agggtgcaaa aagaaaaatt agagaagaag atagacaatg
     6241 gatttcttta gatatgccag ggcaaagaag aacttatgaa ttacaggtaa gatatttctt
     6301 tggcaaacca tggttacctc caaaaggaca acattggggt ttatcgcaac aaaaaataac
     6361 tgaatatgaa aaattggaat ggataagaat taatcccggc agaaaatata ttgatacaca
     6421 aggcaataca gttaaaggaa tgccggaata tcttaaagaa ccagaattac ttttagatac
     6481 aaattggact gatataaaaa gttacgaaac tcataacact ttctttgcca ccgaaaattc
     6541 agaaatacta cttaagagag taattgaggg aatggaatat gacaaacaat taatcataat
     6601 ggatttcttc cttggctctg gcacaaccac cgcagtagca cataaacttg gtagaaaatg
     6661 gattggcgtt gagatgggag aacattttta tagtgtagtt ttgccgagaa tgaagaaagt
     6721 tttggcttat gataaatctg gtatttcaaa agaagtaaag gaatatcagg g
//
LOCUS       DAOOVW010000066         6731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_6668_length_6731_cov_9.687687, whole genome shotgun sequence.
ACCESSION   DAOOVW010000066 DAOOVW010000000
VERSION     DAOOVW010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6731
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6668_length_6731_cov_9.687687"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            408..1742
                     /locus_tag="PLW95_07555"
     CDS             408..1742
                     /locus_tag="PLW95_07555"
                     /inference="COORDINATES: protein motif:HMM:NF025065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOV22510.1"
                     /translation="MDIYKFLGKEFKCEYCGKKHKVDIKFIEKGNIKNLPELISNFYG
                     NGNKILVLCDNITYDVAGKQIEKVIKKENNVESLVLKEKSGKRVIAKEEYLPQIEEYA
                     KTNNLIITVGTGTITDLGKITGDKLNIPVLSFPTAASMNAYTSNVAAYIKNGVKFTIP
                     VKSADGVFMDMQVIKEAPIELTKSGFADSLAKSFANSDWKISSIMTGEEFCSLPYKIV
                     SDVEKQYIDKGDLIIQGDEKTIEVLMEGLNLGGISMMIAGKSSPASGGEHLISHFLDM
                     YAHQYERDVFAFHGIQVASGIYISSLLYDKIKNINKTEIKKMVEKTKINYSEKYEKIV
                     NYFPSGKEILKKEFENKIEILKKLRIKLPEKWDEIKEGVFKIVIPPERIKSVLKKGKV
                     PFHLKDIGVDENLIYDDIIFSRFIRARVTILDIADEIGILENFTNKYIGGVI"
     gene            1739..2440
                     /locus_tag="PLW95_07560"
     CDS             1739..2440
                     /locus_tag="PLW95_07560"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOV22511.1"
                     /translation="MKNTVVVAHRGLSSKYPENTILSFQKAVEIKVDAIELDVRETKD
                     GEIIIMHDEKVDRTTDGNGYVEQLTYNEIKKLDAGKWKGNFKDVKVPSLKEVFENIGN
                     KIYYFIEIKKVNISKVIKLIDDFNLKENVVISSFHIEYLLETRKIVPEMPVAFITGNF
                     PDNINTLIENGIRTLNLYHPEITDEKFIYLTKKGILTHVWTVDEKEDMEKYLNMEIPI
                     ITSNCPDLLLKVLGR"
     gene            2437..3357
                     /locus_tag="PLW95_07565"
     CDS             2437..3357
                     /locus_tag="PLW95_07565"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOV22512.1"
                     /translation="MKEKEIIEFIRKSVITRSKNIIAGIGDDTAVVKYTKNDYLLLTI
                     DSIVENVHFKLNQATYYQIGKKAIAVNLSDIAAMGGIPLYILISIGLPEGEKETIGNL
                     LNGFKYMAKRYKFEIIGGNLTKSNILFIDVCAVGKVEKKYLKLRNGAKEGDLIYVTGN
                     LGASQIKKHLNIKPKIEEGRFLVKNFPISAMIDISDGLSSDLITLSKESNVGFNIFLE
                     KIPLSNEVKKISKTKEEEILHALNDGEDYELLFTIPEKYKNNIPEKINKTKITLIGEI
                     TKDREYKGIYKNREINITASGFDHFPILTS"
     gene            complement(3399..4526)
                     /locus_tag="PLW95_07570"
     CDS             complement(3399..4526)
                     /locus_tag="PLW95_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HOV22513.1"
                     /translation="MRKSYHSVCRWTFNPGKGGFVPSNIRPSFGGEKFTTADFVKLVA
                     DKIRPKLPENIVLGIEVHYDTEINEKNIKQVIGVMKETGIFLGLASPGAHTYWAYGGI
                     SSLDKEERKKANEFGIRAVDIGYEMENTWDKEILPTFVLWNGSWGYDLATPLVKNMYE
                     NLKESIANLCKYEEKKSGKFYMAIEPKPNEGHPAMLLPTVASSILFWKKLHQQYGINI
                     DKKGVNKEFGHSEMIGLDHIYDTVEEIDNNNLVHMHVNSQGYNDGVILGGPGKFDIDN
                     GARVNGMNIAIAKLIQQANYKRWKGHDMQPRPYDNEQQAIERVIRSILSWEACEVVAE
                     KMEISVLTEYMEKRDTAKAEDYIRELVVEAQKVFNQLYIQG"
     gene            complement(4546..5109)
                     /locus_tag="PLW95_07575"
     CDS             complement(4546..5109)
                     /locus_tag="PLW95_07575"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOV22514.1"
                     /translation="MYVLGICGSPRRHGNSELLLDMFLKHVEKEEIKTKKFILNQMRF
                     VACQECENVRNDGRCKIEDDMQKIYPEVENADVIVVASPIFFGSLSAQTKMMIDRFQC
                     QYLAINFFKTYKPKNKKGYFICVEASERNDFIENAKSIIKNFFVTIGAKYSGEIICKG
                     VDKKSEVKEKKECLEKVKEISISLITR"
     gene            complement(5109..5231)
                     /locus_tag="PLW95_07580"
     CDS             complement(5109..5231)
                     /locus_tag="PLW95_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22515.1"
                     /translation="MCKSCGCGRKKESKKYVCKKCGRESNKQEVCCGEKMKENK"
     gene            complement(5261..5641)
                     /locus_tag="PLW95_07585"
     CDS             complement(5261..5641)
                     /locus_tag="PLW95_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012966446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HOV22516.1"
                     /translation="MLNIFQSADWKKEKHVPVIEIIEKNKEKGICLKVSVGKEIPHPN
                     TTSHHIVWIEVYFLPDGSKFPYQIGKVDFLSHGASTEGPDTSSIYTEPILQMKFKTAK
                     EGKILSFSYCNIHGLWENEVELKF"
     gene            complement(5652..6155)
                     /locus_tag="PLW95_07590"
     CDS             complement(5652..6155)
                     /locus_tag="PLW95_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOV22517.1"
                     /translation="MKNITKKVLDEAFAGESMAHMKYLSFAEIAEKEGFSNIARLFRA
                     IAFAEQVHAINHARVLEYIDNTEKNLQTAIEGETYEVEEMYPSFDAIVNLQGEEKAHR
                     SIHYALEAEKIHQLLYSNAKKSVIKGKDVEIDEIYICPVCGYTHIGTPPENCPVCNVS
                     GKKFKKF"
     gene            complement(6208..6687)
                     /locus_tag="PLW95_07595"
     CDS             complement(6208..6687)
                     /locus_tag="PLW95_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOV22518.1"
                     /translation="MERKGKEIVGVDVEELVKLLNKALSDEWLAYYQYWIGAKVVKGM
                     MKEEVIAELTQHAGDELKHAEMIVGRIIQIGGTPVLKPEDWYKLTNCGYEAPEDPGII
                     PILKQNIKGEQCAIKVYQKLLDFVKDKDPITYGMVLSILTDEVEHEDDLINILEDIE"
BASE COUNT         2378 a         1030 c          913 g         2410 t
ORIGIN      
        1 atcttgataa tgtaattcca tatgttttaa aggtgattga tataataacc tacaacagaa
       61 agtataaata attccttcca gaaaaatgga aaagtatttt ttaatttatc tattacttct
      121 ttattctatt ttattaaaaa ccaactcaga gaaaagttat atgcttaact tttttaaaaa
      181 atttaccctc taaaaaacaa tattttccta atacttttac ttgatttgtg ggaagttagg
      241 gtttaaagaa aagaaataaa tcgtttcact atattgatga agtaataaaa tgagaaaaca
      301 tagaatagga taaggaaagg gaagtattta tgtaatttag agtggcgaag ttgacttgtc
      361 aaatttttag atatatgttt taattttaaa catataaaag gagaaaaatg gatatttata
      421 aatttttggg gaaagagttt aaatgtgaat attgtgggaa aaagcataag gttgatatta
      481 aatttataga aaaggggaat ataaaaaatt tacccgaact tatatcaaat ttttatggaa
      541 atggaaataa gatattggtt ttatgtgata atataactta tgatgttgct ggaaaacaaa
      601 ttgaaaaagt aattaaaaaa gagaataatg ttgaatcact tgttttaaaa gaaaaaagtg
      661 gaaaaagagt aatagcaaaa gaggaatatc tacctcaaat tgaagaatat gcaaaaacaa
      721 ataatttaat aataactgtt gggacaggaa caataactga tttaggaaaa ataacgggtg
      781 ataaattaaa catccctgtt ttatcattcc caactgctgc ttcaatgaat gcttatacat
      841 ctaatgttgc tgcatatata aaaaatggag ttaaatttac aattcctgta aaatcagcag
      901 atggtgtttt tatggatatg caggtaatta aagaagcacc aatagaactt acaaaatctg
      961 gttttgctga ttctcttgca aaaagttttg caaattctga ttggaaaata tcctcaatta
     1021 tgacaggtga agaattttgt tctttgccat ataaaattgt cagtgatgtt gaaaaacaat
     1081 atattgataa aggagattta attattcagg gagatgaaaa gacaattgaa gttcttatgg
     1141 aagggcttaa tttaggtggt atctcaatga tgatagcagg aaaatcatct cctgcatcag
     1201 gaggagaaca ccttatatct cattttcttg atatgtacgc acaccagtat gaaagagatg
     1261 tttttgcttt tcatggaata caggttgctt ctggaattta tatttcatcg cttctttatg
     1321 ataaaatcaa aaacataaat aaaacagaga ttaaaaaaat ggttgaaaaa acaaaaatta
     1381 actattctga aaaatatgaa aaaattgtta attattttcc atcaggaaaa gaaattttga
     1441 aaaaagaatt tgaaaataaa atagagatat taaaaaaatt aagaataaaa ctacctgaaa
     1501 agtgggatga aataaaagaa ggtgttttta aaattgttat tccacctgaa agaataaaaa
     1561 gtgttctgaa aaaaggaaaa gtcccttttc atttaaaaga tattggtgtt gatgaaaatt
     1621 taatttatga tgatataatt ttttcaagat tcataagggc tagggtaaca attcttgata
     1681 tagcggatga aatagggatt ttagaaaatt ttactaacaa atacattgga ggtgttatat
     1741 gaaaaacaca gttgttgttg ctcatagagg tttaagtagt aaatatcctg aaaatacaat
     1801 tctctcattt caaaaagcag ttgagataaa agttgatgca attgaattgg atgtaagaga
     1861 aacaaaagat ggagaaatta ttataatgca tgatgaaaaa gtagatagaa caacggatgg
     1921 aaatggatat gttgaacaac tgacatataa tgaaataaaa aaacttgatg caggaaaatg
     1981 gaaaggaaat tttaaagatg tgaaagttcc ttccttaaag gaggtctttg agaatatcgg
     2041 gaataaaatt tattacttta ttgaaattaa aaaagtaaat atctcaaaag ttataaagtt
     2101 gattgatgat tttaatttaa aagaaaatgt tgttatttct tctttccata ttgagtatct
     2161 tttagaaaca aggaaaattg ttcctgaaat gcctgttgct tttattactg gtaattttcc
     2221 tgataatatc aatactttaa tagaaaatgg aataagaact ttaaatttat atcaccctga
     2281 aataactgat gaaaaattta tttatctaac aaaaaagggg attttgacac atgtgtggac
     2341 agttgatgaa aaagaagata tggaaaaata cttaaatatg gaaataccaa taattacaag
     2401 taattgccct gatttacttt taaaagtcct tggaagatga aggaaaaaga aataattgag
     2461 tttataagaa aaagtgtaat aaccagaagt aaaaatatta ttgcaggtat tggtgatgat
     2521 accgctgttg taaaatatac aaaaaatgac tatttacttt tgacaattga ttcaattgtt
     2581 gaaaatgtcc acttcaaatt aaatcaggca acttattacc aaattggtaa aaaagcaatt
     2641 gctgtaaatt taagtgatat tgcagcaatg ggaggtattc ccctttatat actcatttca
     2701 attggtttac cagaggggga aaaagagaca attggaaatt tattgaatgg ttttaagtac
     2761 atggcaaaaa gatataaatt tgaaattatt ggaggtaatt taactaaatc aaatatctta
     2821 ttcattgatg tctgtgcagt tggaaaagtg gaaaagaaat atttaaaatt gagaaatgga
     2881 gcaaaagaag gggacttaat ttatgtcact ggaaatttag gtgcttctca aataaaaaag
     2941 cacttaaata ttaaacccaa aatagaggaa ggtagatttc ttgtaaaaaa ttttcctatt
     3001 tctgctatga ttgatatttc agatggtctt tcttccgatt taataactct ttcaaaagaa
     3061 agtaatgttg gatttaatat atttcttgaa aaaatcccgt tatccaatga agtaaaaaaa
     3121 ataagtaaaa caaaagaaga agaaattttg catgctttaa atgatggaga ggactatgaa
     3181 cttcttttta cgatacctga aaaatacaaa aataacatcc ccgaaaaaat aaataaaaca
     3241 aaaataacac taattggaga aattacaaaa gacagagaat acaaaggaat ttataaaaac
     3301 agagaaataa atataactgc atctggtttt gaccactttc caattctaac ttcctaaact
     3361 ccaactttta tcatatataa taacgagaaa ttttaaaatt atccttgaat ataaagttga
     3421 ttaaaaactt tctgtgcttc tactacaagt tctcttatgt aatcttctgc ttttgctgta
     3481 tctctttttt ccatatattc tgttaaaaca cttatttcca ttttttctgc aactacctca
     3541 catgcttccc atgaaagaat acttcttata actctttcaa ttgcctgttg ttcattatca
     3601 tagggtctgg gttgcatatc atgtcctttc catcttttat aatttgcctg ttgaattaat
     3661 ttcgctattg ctatattcat cccatttact cttgcaccat tgtcaatatc aaatttcccc
     3721 ggtccaccta aaattactcc atcattataa ccctgactat tcacatgcat gtggacaaga
     3781 ttattattat caatttcttc aactgtatca taaatatggt caagtccaat catttcagaa
     3841 tgcccaaatt ctttatttac acctttttta tcaatattta taccatattg ttgatgtagt
     3901 tttttccaga ataaaattga acttgcaacg gttggaagta gcattgcagg gtgcccttcg
     3961 tttggttttg gctcaatcgc catataaaat ttgccacttt tcttttcttc atatttacat
     4021 aaatttgcta tactttcttt taaattttca tacatatttt taactaaagg tgttgctaaa
     4081 tcataacccc atgaaccatt ccagagaaca aaagtaggta atatttcttt atcccatgta
     4141 ttttccattt catatcctat atcaacagcc cttattccaa attcgtttgc tttttttctt
     4201 tcttctttat caagtgaaga tatacctcca tatgcccaat aagtatgagc accaggagat
     4261 gccaaaccaa gaaaaatacc tgtttctttc attactccaa ttacttgttt aatatttttc
     4321 tcattaattt ctgtatcata atgtacctca atcccaagaa caatattttc aggtaattta
     4381 ggtctaattt tatctgctac aagttttaca aaatcagcag tcgtaaattt ttcaccacca
     4441 aaagaaggtc ttatattaga aggaacaaaa ccacctttgc ctggattaaa tgtccatcta
     4501 caaactgaat gataactttt tctcattgta ttctcctttt aaatttcatc tcgttattaa
     4561 ggaaatagaa atttctttaa ctttttcaag acattctttt ttttctttta cttcactttt
     4621 cttatccact cctttacata taatttctcc tgaatatttt gcacctattg ttacaaaaaa
     4681 attttttatt atacttttag cattttcaat aaaatcattt ctttcacttg cttcaacaca
     4741 aataaaatat ccttttttat tttttggttt atatgttttg aaaaaattta tagcaaggta
     4801 ttgacattga aatctatcta tcatcatttt tgtttgagca gataaactac caaaaaaaat
     4861 aggagaggca acaacaatta catctgcatt ttcaacttct ggataaattt tttgcatatc
     4921 atcttctatt ttacaccttc catcatttct tacattttca cactcctgac acgcaacaaa
     4981 tctcatctga tttaatataa atttttttgt ttttatctct tctttttcta catgttttag
     5041 aaacatatca agcaaaagtt cagaattacc atgccttctt ggacttccgc atatgcctaa
     5101 aacatacatt atttattctc cttcattttt tctccacaac aaacttcctg cttattggat
     5161 tctcttccgc atttcttaca tacatatttt ttactttctt tttttcttcc acatccacaa
     5221 cttttgcaca tttctctcac ccccttatag ttaaaaaatt ttaaaacttc aattcaactt
     5281 cattttccca caacccgtga atgttgcaat atgaaaaaga taaaattttc ccttcctttg
     5341 ctgttttaaa tttcatctgt aaaattggtt ctgtataaat agaacttgta tcaggccctt
     5401 ctgttgaagc accatgtgaa agaaaatcaa ctttacctat ttgataggga aattttgacc
     5461 catctggcag gaaataaact tctatccaga caatatgatg agaagttgta tttggatggg
     5521 gtatttcttt accaacactt acttttaaac atataccttt ttctttattc ttttcaatta
     5581 tctcaataac cggaacatgt ttttcttttt tccagtcagc cgactgaaat atattcagca
     5641 tacctacctc cttaaaattt tttaaatttt ttacccgata cattacatac aggacaattt
     5701 tctggaggag tccctatatg agtgtacccg cagacaggac atatatatat ttcatcaatt
     5761 tcaacatctt tgcccttaat aacacttttt tttgcatttg aatacaacaa ttgatgaatt
     5821 ttttctgctt caagtgcata gtgaattgac ctatgtgctt tttcctctcc ctgtaaatta
     5881 actattgcat caaaagaagg atacatttct tctacctcat atgtttctcc ttcaattgcc
     5941 gtctgtaaat ttttttctgt attatctata tattccagaa ctcttgcatg atttattgca
     6001 tgaacctgtt ctgcaaaagc aattgctcta aaaagtcggg ctatatttga aaacccctct
     6061 ttttctgcga tttctgcaaa ggataaatat ttcatatgag ccatactttc accagcaaat
     6121 gcttcatcta aaactttttt tgtaatgttt ttcattttca aaaactccat ttttgtattc
     6181 aaccactgcc aaatttgaca ctacttttta ttcaatatct tcaagaatat ttataaggtc
     6241 atcttcatgc tcaacttcat cagttagaat acttaatacc ataccataag taattgggtc
     6301 tttatcttta acaaaatcaa gaagtttttg atagacctta attgcacatt gttcaccctt
     6361 aatattttgt tttaaaatag ggataatacc agggtcttct ggtgcctcat atccacaatt
     6421 tgttaattta taccaatcct ctggttttaa aacaggagtt ccaccaattt gaataattct
     6481 gccgacaatc atttcagcat gtttcaactc atcacctgca tgctgagtaa gttcggcaat
     6541 tacttcttct ttcatcatcc ctttaacaac ctttgctcca atccagtact gataatatgc
     6601 aagccattca tccgaaagtg ccttattaag caattttact aattcttcta catctactcc
     6661 aactatttct ttgccttttc tttccatact ttcctccttt tttggttatt ttttaaatag
     6721 ttcaattgct t
//
LOCUS       DAOOVW010000067         6594 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_6835_length_6594_cov_8.052455, whole genome shotgun sequence.
ACCESSION   DAOOVW010000067 DAOOVW010000000
VERSION     DAOOVW010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6594
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6835_length_6594_cov_8.052455"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(663..744)
                     /locus_tag="PLW95_07600"
     tRNA            complement(663..744)
                     /locus_tag="PLW95_07600"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(709..711),aa:Leu,seq:tag)
     gene            863..1639
                     /locus_tag="PLW95_07605"
     CDS             863..1639
                     /locus_tag="PLW95_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOV22519.1"
                     /translation="MGIIIFIFGLIFGSFANVCIYRLPKGKSIITPGSFCPNCKKSIL
                     WYDNIPLLSYIILKGKCRYCGEKISQRYFIVELLTGILFLLNYNVFGLTSSFFIYTLF
                     IFSLIIISFIDIETFLISDVIVIPCIFLGLLFSSFFPMMHHFSGKLEGLLYSLEGLIL
                     GVGLLLFIAFAGKIAFKKDAMGGGDIKLLGMIGAFLGWKCVFLTIFFSSLLGTIISLV
                     LIGLKKKKIDDYIPFGPYLGLGAVISLFFKGHDFLGFFIN"
     gene            1644..2159
                     /locus_tag="PLW95_07610"
     CDS             1644..2159
                     /locus_tag="PLW95_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22520.1"
                     /translation="MSYPSFEIVKEFFTIFDFFIIEKEDVIFVKNLRVEKEDIKNFIL
                     KKEDISKIEQAIVKPIAWHTQKFTPSVLNKFPEIFDFFKPDFLKDENFRKILVIPGLP
                     STENLKKKSIEIINDKGIDNIILFPTVISSLVEKINSRKVYLSFTNEILRILKFYDFF
                     SETMELPFKKK"
     gene            2156..4177
                     /gene="recG"
                     /locus_tag="PLW95_07615"
     CDS             2156..4177
                     /gene="recG"
                     /locus_tag="PLW95_07615"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008165.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOV22521.1"
                     /translation="MKITLNTSLLEIPSVGEKKYKLFKKLNIFTVEDLLFYFPRKYID
                     LRNIKKIYQIQPGDIVTIKGNVIAVEEKRRWGRIPYLKVAISDGTGILYLVFFNQTYL
                     KNIFKAGEKFFIYGGVEKFNNQLEIVSPFYEKEENGKIDYILPVYPLTEGLSQRYLRK
                     ILKFLLENLLEYPPEFLPIYERQKYGFSNIKHALKNIHFPFSEINLQKSRSYLIFREF
                     FILQLNLLWKKNIEQNIIKEKLPDIKIDEKIIDIFQAKIPFKLTKGQIDVIGDIINDL
                     KKEKLIRRLIHGEVGSGKTVIAIFALWFFARSGYQAILLAPTEILAEQHYLNWQEFFL
                     MQDINVSLLVGQLSEKEKEEMREKIKSGEIQVVIGTHALLSEKVEFKNLRMVVIDEQH
                     KFGVEQREILKQKGEVVHHIVMSATPIPRSVALTIYGDMDFSTLGDLPKGRRDVITYL
                     FHEKEKNKIYSFIEYQNKKREKGFIITPSIKENEDIKSAEEEYEKIRNILPDVKVGLI
                     HGKLSQEEKEKTMQMFRNGQLQLLVATTVVESGIDVPEASFIIVEQAERFGLAQLHQL
                     RGRVGRSGNTGYCFLVVYTDNKEIIERIESFIEAESGFEIAEIDLKLRGPGDLLGTRQ
                     HGILPLKIGDIMKDMELLKIARKKVEEILSDDPKLEKEKNKIIRTFINV"
     gene            4170..4853
                     /locus_tag="PLW95_07620"
     CDS             4170..4853
                     /locus_tag="PLW95_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOV22522.1"
                     /translation="MYKEIAVVIPAYNEEKKIGEVVKEAKKLFEKVIVINDGSTDKTE
                     EIAKNQGAIVISHSKCMGKGTALKTAFSYIISEGIPAFITIDGDGQHLISDSENFIEK
                     YKKIKRGGIFIGKRKMVSTKMSFLRRITNTSMSLLISLLSFQWIPDTQCGYRLIKKDV
                     IEKVKLITSHYETESELLIKASWKGFRIVSVPITTVYRDEKSKIKPGRDTLRFFKMIL
                     VIIFPQLSK"
     gene            4870..5508
                     /locus_tag="PLW95_07625"
     CDS             4870..5508
                     /locus_tag="PLW95_07625"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOV22523.1"
                     /translation="MNFEILRKQMVETQLIPRGIKDRKVIDAFLKVPREKFVPDDIKK
                     FAYDDTPLPIGEGQTISQPYIVALMTSLLEVKDGEKILEIGTGSGYQTAILCEIGCDV
                     YSVEKIPALAMRAKNILQQLNYKVKITIGDGTLGWEEYAPYDGIIVTAGGPKIPEALL
                     SQLNENGKLVMPVGDIFSQDLIRVKKSKGKFIKENFGACQFVPLKGENGWQE"
     gene            complement(5603..6283)
                     /locus_tag="PLW95_07630"
     CDS             complement(5603..6283)
                     /locus_tag="PLW95_07630"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOV22524.1"
                     /translation="MNITIGEEIFRIFLSGVLSGIIGFEREKHGREAGLRTMFLVGIG
                     VTTILISSFNVYNVFSNVESPILSIDPARIAYGVVTGIGFLGAGAIIKDTKRIRGLTT
                     AACLWVVTSIGLAVGCGLYTISLFTTFFSLFILYALKIIEHKILPDIYSTLTIVVSQE
                     NEKTLSELKTLLKNFQMKILSISFERDIENKISSFTFILRYNREKLVEELIKKLTNNK
                     DIKKIIWK"
     gene            complement(6345..>6594)
                     /locus_tag="PLW95_07635"
     CDS             complement(6345..>6594)
                     /locus_tag="PLW95_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22525.1"
                     /translation="FLCLLRFLYVFISLSPLIYLLPAGEKERQSPLIFTFSPAGRRNS
                     ILFLPLPFFFLPSPLWGEGKGEGEFHLPFICFLTLPSL"
BASE COUNT         2441 a          853 c         1158 g         2142 t
ORIGIN      
        1 aagggtgagg ggggtattaa tttctattat acatttgatg taaaaacagt atgaatatca
       61 taatgcctaa aaatatggga atttaggtca aagtaaaaat tgaggtgcga ggggagggaa
      121 aagtttatcc gccaattact atggcggaaa ccctcatggg gttgctctcg ctcttgggct
      181 attccttact cataagtttc cccttgatta aaccgactaa agttgcttcc cttgtttctc
      241 atagttgatg cttgttttcc ccctttgaca aaccgactaa agtagcaaag tttagcacac
      301 agagttggtg ccgtaatgta tttcaatagg cacaacgggg tagggggatt tagaaatcca
      361 cccccgccct cctttgtcaa aggagggagc aatcggagaa aaaaggtgga aagaagggga
      421 tttgaagggg gatttaggat taaaagccat cccatccttc ctttttaaaa ggaaggagaa
      481 taatgaacaa aataggataa aagggaatct ctttatacaa gaggatttta gaagatgtgg
      541 gtaagggata gggtgtatac catacgggcg agcatttcct aacgataccc ccaaaacgaa
      601 aagcgatgga aaacaacttt ttcccgttcc ctttttatcc cctgagtgtt cttttaatta
      661 ggtgcgagga gagggagttg aaccctcacg ggataaaccc actggatcct aagtccagcg
      721 cgtctgccat tccgccaccc tcgctcttgt tctttctaat agaaatttat cattaaaaat
      781 tatatggttt ttctctttat ttttcaactt aaccttttta aaaaattttt acagagttgg
      841 aaatttataa tataatttca gtatgggaat aataattttt atatttggac ttattttcgg
      901 tagttttgct aatgtatgta tttatagatt accaaaagga aaatcaatta taacaccggg
      961 ttctttttgt cctaattgta aaaaaagtat tttgtggtat gataacatcc ctcttttaag
     1021 ttatataatt ctaaaaggga aatgtagata ttgtggagaa aaaatttctc aaaggtattt
     1081 tattgttgaa cttttaactg gaattttatt tcttctaaac tataatgttt ttggattaac
     1141 ttcttccttt tttatttata cactttttat tttttctctt attataatta gttttattga
     1201 tattgaaaca tttttgattt cagatgttat tgtaatacca tgtatttttc tggggcttct
     1261 tttttcttct tttttcccga tgatgcatca tttttctgga aagttagaag gacttttgta
     1321 ttcacttgaa ggacttatac ttggggtcgg cttacttctt tttattgctt ttgcaggtaa
     1381 aattgctttc aaaaaagatg caatgggggg aggggatatt aaacttcttg gtatgattgg
     1441 tgcttttctt ggctggaaat gtgtttttct tacaatattt ttttcttctc ttcttgggac
     1501 tataataagt ttagttttaa ttggtttaaa gaagaaaaaa attgatgatt atatcccatt
     1561 tggtccttat cttggtctgg gtgcagttat aagtttgttt tttaaagggc atgattttct
     1621 tggattcttt ataaattaaa aaaatgagtt atccaagttt tgaaattgta aaggagtttt
     1681 ttacaatatt tgatttcttc attattgaaa aagaagatgt tatttttgtg aaaaatttaa
     1741 gggttgaaaa agaagatatt aaaaacttta ttttaaaaaa agaagatatt tcaaaaatag
     1801 agcaggcaat tgtaaaacca atcgcatggc atactcaaaa atttactcct tctgttttaa
     1861 ataaatttcc tgaaatattt gattttttta aacccgattt tttaaaagat gaaaatttca
     1921 gaaaaatact tgtaattcct ggcttacctt caactgagaa tttaaaaaag aaaagtatag
     1981 aaataataaa tgacaaagga atagacaata ttattctttt tccaactgta atttcatctc
     2041 ttgtggaaaa aataaattca agaaaagttt atttatcatt tacaaatgag atattaagaa
     2101 ttttaaagtt ttatgatttc ttttcagaaa caatggaact acctttcaag aagaaatgaa
     2161 aataacctta aatacatcac ttttagaaat accctcagtt ggagaaaaaa aatataaact
     2221 atttaaaaaa ttaaatattt ttacagttga ggacttactt ttttatttcc caagaaaata
     2281 tattgattta agaaacatta aaaaaatata tcaaattcaa cccggtgata ttgtaactat
     2341 aaaaggaaat gttattgcag tagaagaaaa aaggagatgg ggaagaatac cttatctaaa
     2401 agttgctatt agtgatggaa ctggtatttt atatcttgtt tttttcaatc agacatattt
     2461 aaagaatata tttaaggcag gagaaaaatt ttttatatat ggtggagttg agaaatttaa
     2521 taaccagttg gaaattgttt caccctttta tgaaaaagaa gaaaatggaa aaattgacta
     2581 tattttacct gtttatcctc ttacagaagg gttgtcacag cgatatttaa ggaaaatttt
     2641 aaaattttta ttggaaaatc tattggaata tccacctgaa tttttaccaa tttatgaaag
     2701 gcaaaaatat ggtttttcaa atataaaaca tgctcttaaa aatatacatt tccctttttc
     2761 tgaaataaac cttcaaaaaa gcaggagtta tttaattttt agagaatttt tcatcttaca
     2821 attaaattta ttatggaaga aaaatattga acagaatatt ataaaagaaa aacttcctga
     2881 tataaaaata gatgaaaaaa taatagatat ttttcaggca aaaattcctt ttaaacttac
     2941 aaaagggcaa atagatgtta ttggggatat tataaatgat ttaaaaaagg aaaaacttat
     3001 aagaaggtta atacatggtg aagttggttc aggtaaaact gtaattgcaa tttttgcctt
     3061 atggtttttt gctcgttcag gatatcaggc aattttactt gctcccacag aaattcttgc
     3121 tgaacaacat tatcttaact ggcaggaatt ttttttaatg caggacataa atgtttcact
     3181 tcttgtgggg cagttatcag aaaaagaaaa agaagaaatg agagagaaaa taaagagtgg
     3241 ggaaattcaa gttgttattg gaacacacgc tttattaagt gaaaaagtgg agtttaagaa
     3301 tttaagaatg gtagttattg atgaacaaca caaatttggg gttgaacaga gagaaatttt
     3361 aaagcagaaa ggcgaagtag ttcatcatat tgttatgagt gcaactccca taccgagaag
     3421 tgttgcacta acaatttatg gtgatatgga tttttctaca ctcggtgatt taccaaaagg
     3481 cagaagagat gttattacat atctcttcca tgagaaagaa aaaaacaaaa tatattcttt
     3541 tattgaatat caaaataaaa aacgagaaaa agggtttatt ataactcctt caataaaaga
     3601 aaatgaggat ataaaatcag cagaagaaga atatgaaaaa ataagaaata ttttaccgga
     3661 tgtgaaagtt ggacttatac atggcaaatt atctcaggaa gaaaaagaaa aaacaatgca
     3721 aatgttcaga aatgggcaat tacaacttct tgttgctaca actgttgttg aatcaggaat
     3781 agatgttcca gaggcaagtt ttataattgt agaacaggca gaaaggtttg gacttgctca
     3841 acttcaccaa ttgagaggaa gagttggcag aagtggcaac accggttatt gttttcttgt
     3901 tgtttatact gacaataaag aaattattga acgaattgaa agttttattg aggcagaaag
     3961 tggatttgaa attgcagaaa ttgaccttaa attaagaggt cctggggatt tactaggaac
     4021 aagacaacat ggtattctgc ctttgaaaat aggtgatata atgaaagata tggaattatt
     4081 aaaaattgca agaaagaaag ttgaagaaat actttctgat gacccaaaac ttgaaaaaga
     4141 aaaaaataaa attataagga cttttataaa tgtataaaga aattgcagta gttattcctg
     4201 cttacaatga agaaaaaaag ataggtgaag ttgtaaaaga ggcaaaaaaa ttatttgaaa
     4261 aagttattgt tataaatgat ggttcaactg ataaaacaga ggaaattgca aaaaaccaag
     4321 gagcaattgt tatttctcat agtaaatgta tgggaaaagg gacggctttg aaaactgctt
     4381 tttcatatat tatttctgaa ggcatacctg cttttataac aatagatggt gatggacaac
     4441 acttaatttc tgattcagaa aattttattg aaaaatataa aaaaataaaa agagggggaa
     4501 tttttatagg gaaaagaaag atggtttcta caaaaatgtc ttttttaaga agaattacaa
     4561 atacttcaat gtctctttta atttctctgt tgtcttttca atggataccg gatactcaat
     4621 gtggataccg ccttataaaa aaagatgtga ttgaaaaagt aaaacttata acatcccatt
     4681 atgaaacaga atcagaactc ttgattaaag catcgtggaa gggatttaga atagtttctg
     4741 ttccgattac aaccgtttat agagatgaaa agagcaaaat taaaccaggc agagatacat
     4801 taagattttt taaaatgatt ttagtaataa ttttcccgca attatcaaag taaaattatg
     4861 gaagaaaaaa taaattttga gattttaaga aaacagatgg ttgaaacaca acttattcca
     4921 cgcggtataa aagacagaaa agttattgat gcttttttaa aagtaccaag agaaaaattt
     4981 gtccctgacg atataaagaa atttgcctat gatgatactc cacttcctat tggagaagga
     5041 caaacaataa gtcagccata cattgttgct cttatgacat ccttacttga agttaaagat
     5101 ggcgaaaaaa tacttgaaat aggtacaggc agtggctatc aaacagcaat tttatgtgaa
     5161 attggttgtg atgtatattc agttgagaaa attccagcac ttgcaatgag agcaaaaaac
     5221 atattacagc aattaaatta taaagttaaa ataacaatag gagacggaac attgggttgg
     5281 gaagaatatg ctccgtatga tggaataatt gtaacagcgg gtggacctaa aattccagaa
     5341 gcattacttt cacagttaaa tgaaaatgga aaacttgtta tgccagttgg agatattttt
     5401 tcacaggatc ttataagagt aaaaaaatca aaaggcaaat ttattaaaga aaactttggt
     5461 gcctgtcagt ttgttcctct aaaaggggaa aatggctggc aggaataatt aaaaagaggc
     5521 acaagaagaa atccattgta aatttaaaat taaaaacgaa aaatgtaaaa ttataactca
     5581 aaatttaaat tagatgtcta cattatttcc agataatttt ttttatgtct ttattatttg
     5641 taagtttttt tatcaattct tctactaatt tttctctatt atatcttaaa atgaaagtaa
     5701 aagaagaaat tttattttct atgtctcttt caaaagaaat tgatagaatt ttcatctgaa
     5761 aattttttaa aagtgtcttt aattcagata gcgttttttc gttttcctga cttactacaa
     5821 ttgttaaagt gctataaata tcaggtaaaa ttttatgctc aattattttt aaagcataaa
     5881 ggataaaaag ggaaaaaaat gttgtgaaaa gagaaatagt atataaacca caaccaactg
     5941 caagtccaat acttgtaacc acccacaaac atgcagcagt tgtaagtcct ctaatccttt
     6001 ttgtatcttt aattattgct cctgctccaa gaaagccaat gcctgtaaca actccataag
     6061 caatacgagc agggtcaata ctcaatattg gactttcaac atttgagaag acattataaa
     6121 cattgaaaga agaaattaaa attgtggtta ctcctattcc tacaagaaac attgttctta
     6181 aacccgcttc tcttccatgt ttttctcttt caaatccaat aataccactc aaaacgccag
     6241 atagaaaaat tctaaaaatt tcttccccaa tagttatgtt cataacttaa ttatatcaat
     6301 tctttcagat agttcaaata aatttgaaaa agaattataa aaggttataa tgaaggtagt
     6361 gtcaaaaaac agatgaaagg gagatgaaat tccccctcac ccttaccctc tccccaaagg
     6421 ggagagggaa gaaagaaaaa agggagaggc agaaagagga tggagttcct tctccccgcg
     6481 ggggagaagg tgaagatgag cggggattgt ctttccttct ccccagcggg gagaagatag
     6541 atgagggggg agagggatat aaagacatat aaaaacctta aaagacaaag aaag
//
LOCUS       DAOOVW010000068         6014 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_7595_length_6014_cov_8.624769, whole genome shotgun sequence.
ACCESSION   DAOOVW010000068 DAOOVW010000000
VERSION     DAOOVW010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6014
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7595_length_6014_cov_8.624769"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..958)
                     /locus_tag="PLW95_07640"
     CDS             complement(29..958)
                     /locus_tag="PLW95_07640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOV22526.1"
                     /translation="MKKLTRILLVKSGITRKPKDQYGYWEGIISIGINLILFGIKYWA
                     GIVSKSIAVKADAFHTLSDVITSVLVIVGFFFALQPPDKKHPFGHGRSEKVATIVMAT
                     LLCVIGYEFFISSLNQFLHPAEIQFRVLFVVVFFISILLKEFLYFVSLIMYEESGYEA
                     LKADAWHHRSDSLASLIVILGLFFFKAGLVKLDGILGMLIALLIVYTGLVLILEVGSF
                     LLGEAPSPDLEEQIQKIVKNIGGQGIHHIHIHDYAGKLEITLHVRMNPNVSLEQVHKK
                     ATSIEQAIKAEIPNCEVTVHSEPENLKSNPTKK"
     gene            complement(1303..3201)
                     /gene="dnaK"
                     /locus_tag="PLW95_07645"
     CDS             complement(1303..3201)
                     /gene="dnaK"
                     /locus_tag="PLW95_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014405748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOV22527.1"
                     /translation="MKEKIIGIDLGTSNSSAAVMEGDKPVIIPSAEGTTIVGGKAFPS
                     YVAFTKDGQILVGEPARRQAAVNPEGTVFAAKRKMGTDYKYNIYGKTYTPEQISAFIL
                     QKIKKDAETFLGVPIKKAVITVPAYFNDNQRQATKDAGTIAGLEVVRLINEPTAAAFA
                     YGLNKLDQELKFLVFDFGAGTLDVTIMEMMRGVFKVLSTSGNTQLGGTDMDNVLIDYI
                     VGEFKKETGIDLKQDKMAIQRIREAAEKAKIELSSTVETDINLPFITADQTGPKHLVM
                     KIRRAKLEELIQPIIDKCKEPIDRAIEDAKLTRSEIKKIILVGGPTRMPIVQKFIEEY
                     IGVKPERGVDPMECVSIGAAIQGAILAGEMKDKDILLLDVTPLSLGVETLGGVFTKVI
                     ERNTTIPTKKSQIFTTAADNQTSVEVNVLQGERPMAKDNLSLGKFHLDGIPPAPRGVP
                     QIEVSFDIDANGILHVSAKDLGTGKEQSIRITASKKLSQEEIEKMVKEAEKHAEEDKK
                     ARELIDLCNQADGLIYSVNKTLKEHGDKLNDAEKKAIEEKIKNLDNLIKSETKDAESI
                     KTGMSELEQASHKLAQILYQQAQSSQQQQQTETPTSEQQPTEEKKEDKGNVVDAEYKE
                     VDDDKKNE"
     gene            complement(3537..4622)
                     /locus_tag="PLW95_07650"
     CDS             complement(3537..4622)
                     /locus_tag="PLW95_07650"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOV22528.1"
                     /translation="MIITNITPLRYSPGKLEYFSFIRGDVGAQFHQGPEIYPFSDGRV
                     LILWGAYDIQECSKDSVVLYSESDDQGITWSAPQVFLKTPGCLNSALLLQMRNTPKVL
                     LFIRESFYVDYVEDKKHRIFTKWADYSKSYSRMILRESNDLGKTWSLGREMPFYCFEA
                     LQIESGRILVSVYSLDKSKKEQFYIAYFLYSDDEGKTWEKSNDIIMPEKRGAMEPTFV
                     EVSPNKIYCIIRNKSGYLYESISEDGGKTWQSAKKTQIPAPESMARLLKLKSGNVLLV
                     WNNIPSTSQHPRHPLVTSLSCDGCESWSEPKIIADDIGLNQLSNHGISQLKDGRILLG
                     ISHYYATLPSFGDLEMAIFDEEWMSSR"
     gene            complement(4619..5773)
                     /locus_tag="PLW95_07655"
     CDS             complement(4619..5773)
                     /locus_tag="PLW95_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22529.1"
                     /translation="MDIYLECFSSLGNSVEHGQKIPVAKTLFWPDEEIYRIVNEMKRY
                     NFSVSIPYTDPFPYALFNLKGRKIRDFKVAHDEYLARIKRFVEYAHDEKIKVFLYFPV
                     GIASISTGYYKEKSTQLQKRYLLGYEIMEHFPTSFTYDCNGFSSWERLVEGKDNIAFM
                     SLANFDVRSYFAESYLTIVENVDADGIQLEPLSMPLDKDGVCIFGYEKSMVNAFIENF
                     GRKPKNNNDSDWVIFRGNCAIEFVKEIKEKLFSLKHPIEFSTVGYSLKDDIRHFWPWQ
                     KMIKEGLIDAFYFRTTSSENLQERIREVKETCEANNPYCRFVGCLDIKNYSQFKNEKE
                     IANGVKKILNSGAKFLGIYGLRWTKFFNNPKERDLKIWENTIKQLQGGVL"
BASE COUNT         1758 a         1169 c          881 g         2206 t
ORIGIN      
        1 ccccgcggtt atactggacg gttaaatttt atttttttgt tgggttggat tttaaatttt
       61 ctggttctga atggacggta acttcgcaat taggaatttc tgccttaatt gcttgctcaa
      121 ttgaagtagc ttttttgtgt acctgttcaa gagaaacatt tgggttcatc cttacatgca
      181 gggtaatttc caatttgcca gcatagtcgt gaatatggat atgatgaatt ccctgtccac
      241 ctatgttttt aactattttc tgaatttgtt cttcaaggtc gggagatggt gcttctccta
      301 aaagaaaact accaacttcc aagattaaaa ccagtcctgt ataaacaata agaagggcaa
      361 ttaacatacc taatattcca tctaatttga ccaggccggc tttgaagaaa aaaagaccca
      421 atataactat aagactggca agggaatcac tgcgatgatg ccaggcatct gcttttaatg
      481 cttcataacc actttcttca tacataatta aagagacaaa ataaaggaat tctttgagca
      541 atatagaaat gaaaaacaca accacgaata gaacccggaa ctgtatttct gcaggatgta
      601 aaaattgatt caaagaagaa ataaaaaatt catatccaat cacacacaga agtgttgcca
      661 ttacaatcgt agcaactttt tcgctccgtc catgtccaaa aggatgcttt ttatctggtg
      721 gttgtaaagc aaagaaaaaa ccaacaataa ccagaactga tgtaattaca tcagacaaag
      781 tatgaaatgc atctgctttg acagcaatac ttttgctaac aattccagcc cagtacttaa
      841 ttccaaataa aatcaggttt atgccaatac ttataattcc ttcccagtat ccatattggt
      901 cctttggttt tcgtgtaata cctgatttta ctagaagaat tcttgttagt tttttcattt
      961 ttttccactc agtatttagt ttgattattg cctatttgaa tttttaacta ttgacaatat
     1021 atatttattt taaatatgtt atatgataac aaacaaataa agataaatca aacaaggaca
     1081 taaattgaaa gagatgctta ctatatttct ggtcatcagg attcctctgg ggggtaattc
     1141 cgcaggctca tctatcgggc tatccccagt aggagcctgc ggatacagag gaacagagat
     1201 atatcctcac aatagagaga gtgaattttt cattgcctgc cccgcctttt ggcggattaa
     1261 atccaaaagt gtataaattt acaccctatc gttatatttt tgttattcgt tctttttgtc
     1321 atcatcaact tctttatatt cagcatctac tacatttcct ttatcttctt tcttctcttc
     1381 tgttggttgt tgttctgatg taggtgtttc tgtctgttgc tgttgttgtg atgattgtgc
     1441 ctgttgataa agtatttgag caagtttatg agatgcttgt tctaattcac tcattcctgt
     1501 ttttatactt tctgcatctt ttgtctcgct ctttattaaa ttatctaaat tttttatttt
     1561 ctcctctata gcttttttct ctgcatcatt taatttatca ccatgctcct ttaatgtttt
     1621 atttactgaa tatatcaatc catctgcctg attgcacaaa tcaataagtt ctctggcttt
     1681 tttgtcttct tctgcatgtt tttctgcttc ttttaccatt ttttctattt cttcttgtga
     1741 taatttttta gaagcggtta ttcttattga ttgttctttt ccagttccta aatcctttgc
     1801 agaaacatgt aaaataccat ttgcatcaat atcaaaacta acttcaattt gaggtactcc
     1861 tcttggtgcg ggtggaatac catctaaatg aaatttacct aaacttaaat tatcttttgc
     1921 cataggtctt tcaccttgaa gaacatttac ttccacagat gtctgattat ccgcagcagt
     1981 tgtaaatatc tgactttttt tcgtagggat tgttgtgttt ctttctatta cttttgtaaa
     2041 aactcctcct aatgtttcta cccctaagga aagaggggtt acatctaaaa gcaatatatc
     2101 tttatctttc atttctcctg caagaattgc cccttgaatt gctgctccta ttgatacaca
     2161 ttccataggg tcaactcctc tttctggttt tactccaata tattcttcaa taaatttctg
     2221 aactattggc attcttgttg gtccaccaac aagtattatt ttcttaattt cgctccttgt
     2281 taattttgca tcttcaattg ctctatcaat tggttcttta catttatcta taattggctg
     2341 gatgagttct tccaattttg ctcttcttat tttcattaca aggtgtttgg gtcccgtctg
     2401 gtcagcagtt ataaatggca gatttatatc tgtttcaaca gttgaagaaa gttctatttt
     2461 tgctttttct gctgcttctc ttattctctg tattgccatt ttatcctgtt ttaggtcaat
     2521 acctgtttct tttttaaatt cccctacaat gtaatcaatt aagacattat ccatatctgt
     2581 accgcccaat tgtgtatttc cacttgttga tagaacttta aaaacacctc tcatcatttc
     2641 cattattgtt acatcaagtg ttcctgcacc aaaatcaaaa acaagaaatt ttagttcctg
     2701 gtcaagttta ttgagaccat aggcaaaagc agctgctgtt ggttcgttta ttaatcttac
     2761 aacttcaagc cccgcaatag ttcctgcatc ttttgttgcc tgtctctggt tatcattaaa
     2821 atatgctgga actgttataa ctgctttctt aataggaact ccaagaaatg tttctgcgtc
     2881 tttctttatt ttctgcagaa taaaagcaga aatttgttca ggcgtgtatg ttttcccata
     2941 gatattatat ttataatcag ttcccatttt tctttttgct gcaaagacag ttccttctgg
     3001 attaactgct gcctgtcttc ttgctggttc tccaacaaga atttgtccat cttttgtgaa
     3061 agcaacataa gaagggaatg ctttccctcc aactattgtt gttccttcag cacttggaat
     3121 tataactggt ttatctccct ccatcactgc tgctgaggaa ttacttgttc caaggtctat
     3181 tccaataatt ttttccttca tttttaccac ctccattttg taaatatcat taaatcctgt
     3241 ttatatttaa gtaacaaaag aaaaaacttt ctttaaaatc catcctaacc ttccctcggc
     3301 catagccacg ggcgaggcct ttttaaagga aggaactttc cccttttttt aagggaggat
     3361 ggggaaattt ttctttgttt ttatcttact acttatctac gggatagcct gtagactacg
     3421 cccgagttaa tcctgcggac tactctctct acttacagtc aacgaaagtt gactgagtag
     3481 ttgatattta gatgatagag tcgtttaaaa gtttcagtaa atatataact atttaatcaa
     3541 cgagaactca tccattcctc atcaaaaatt gccatttcta aatcaccgaa ggatggtagt
     3601 gttgcatagt aatggctaat gcctaataga attctaccat cttttaactg tgatattcca
     3661 tgattactta gttggtttaa tccaatatca tcggcaataa ttttaggctc actccaactt
     3721 tcacaaccat cacaagaaag tgaggtaact aaagggtgtc ggggatgttg agaagttgat
     3781 ggaatattat tccaaactaa aagaacattt ccacttttaa gtttaagtaa tcttgccatt
     3841 gactcaggag cagggatttg tgtctttttt gcagattgcc atgtttttcc tccatcttct
     3901 gagatagatt cgtaaagata tccactctta tttcggataa tacagtaaat tttatttggt
     3961 gaaacttcaa caaatgttgg ttccatcgct cctctttttt ctggcattat aatatcattg
     4021 cttttttccc atgttttgcc ttcatcgtca gaatacaaaa aataggcgat ataaaactgc
     4081 tcttttttgc ttttgtccaa ggaataaacg ctaacaagaa ttctgccact ctcaatttgt
     4141 aatgcctcaa aacagtaaaa tggcatttct ctgcctaaac tccatgtttt tcctaaatca
     4201 ttagattctc tgagaatcat acgggaataa ctttttgaat aatcagccca ttttgtaaat
     4261 atccggtgtt ttttatcttc cacataatca acataaaaac tctctcttat gaataataag
     4321 acctttggag tatttctcat ttgaagcaat aatgcactgt tcaagcaacc gggtgttttt
     4381 aagaacactt gaggggctga ccatgttatt ccttggtcat ccgactctga atataaaact
     4441 acactatctt ttgaacactc ttgaatatca taagcacccc ataatattaa aactctacca
     4501 tcagaaaaag gataaatctc aggtccttga tgaaattgtg ctcctacatc tccacgaata
     4561 aaagaaaaat actccaactt tcctggggaa taccttaacg gtgtaatatt tgtaattatc
     4621 ataaaacacc tccttgcaat tgttttatag tgttttccca gatttttaaa tctctttcct
     4681 taggattatt aaaaaattta gtccatcgta atccatatat tcctaaaaat ttagctccac
     4741 tatttaaaat ttttttaacc ccatttgcaa tttccttttc atttttaaat tgtgagtaat
     4801 tcttaatatc caaacaacca acaaatctac aataaggatt atttgcctca caggtttctt
     4861 ttacttctct tattctttct tgtaaattct ctgaagaagt tgtccgaaag taaaatgcat
     4921 caatcaatcc ttcttttatc atcttttgcc aaggccaaaa gtgccttata tcatccttta
     4981 aactatatcc tactgtagaa aattctattg gatgctttaa agaaaataat ttttctttaa
     5041 tttctttaac aaattcaata gcacaatttc cacgaaaaat aacccaatct gagtcattat
     5101 tatttttggg ttttctccca aaattttcta taaaagcatt taccattgat ttttcatatc
     5161 caaagataca aactccatct ttatcaagtg gcatagataa cggttctaat tgtataccat
     5221 cggcatccac attttcaaca attgttaaat aactttcagc aaaataagag cgaacatcaa
     5281 aatttgctaa actcataaag gcaatattat ctttaccttc aactaatcgc tcccaggaag
     5341 aaaaaccatt acaatcataa gtaaaagaag taggaaaatg ttccattatt tcatatccta
     5401 ataaatatct cttctgaagt tgtgttgatt tttctttata atatcctgtt gaaatacttg
     5461 ctattcctac gggaaaataa agaaaaactt ttattttttc gtcatgtgca tattcaacaa
     5521 accttttaat tcgtgccaaa tattcatcat gtgctacttt aaaatctctt atttttctgc
     5581 ctttgagatt aaaaagagca taagggaaag ggtctgtata aggaatagat actgaaaaat
     5641 tatatctttt catttcattg actatccgat aaatctcttc atctggccaa aacaaagttt
     5701 ttgcaacagg aattttttgt ccatgttcaa cagaattacc caaactacta aaacattcta
     5761 aataaatgtc cataaatatt ttgggagtat tgttatggtc tgtgaattct taataggtgg
     5821 tgcaggactt tgcctgtccg ccacgctttg tggcggaagc cctcgggcgt agccctacgg
     5881 gcgagcctgc gccgtcgctg tcgcttctta aagggaaggg cgattcccct ccctttaatt
     5941 tttctctcca ccaaaaccct tataaatatg ggaaaaagtt ttttcccata ccttttcagc
     6001 cgcaggagtt atta
//
LOCUS       DAOOVW010000069         5782 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_7899_length_5782_cov_7.703510, whole genome shotgun sequence.
ACCESSION   DAOOVW010000069 DAOOVW010000000
VERSION     DAOOVW010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5782
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7899_length_5782_cov_7.703510"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="PLW95_07660"
     CDS             complement(<1..773)
                     /locus_tag="PLW95_07660"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008868914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOV22530.1"
                     /translation="MVSELEEKLTNNKFVITSEVGPGKGTNLKFLEEVEVLRGKVDAL
                     NVTDLQSSVMRLGSLAVSHLLIEKGFEPIFQITCRDRNRLALQSDLLSAYVLGIRNVL
                     CLTGDHTTLGDHPDSRPVFDLDSVSLLKTASCLIEGKDIVGNQLDGKPEFFLGAVVSP
                     CYEPVELQVIKMEKKIEAGAKFFQTQAVYDISIFEKFIKMTENYKVPVLCGIVLLKSA
                     GMAKYMNENVAGVSVPEKYIKMMAEAKKEERAKVSIQIAT"
     gene            complement(1587..2231)
                     /locus_tag="PLW95_07665"
     CDS             complement(1587..2231)
                     /locus_tag="PLW95_07665"
                     /inference="COORDINATES: protein motif:HMM:NF023646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOV22531.1"
                     /translation="MLATKIKNEDEILKYLEKKVIVFKCFGCKEVYFPEEGINEFLKK
                     NNQNIIEIIKIDYLCNEEFAETIVNKNIKKFEKCENIIVFSCGVGVQVISKILIEDKK
                     VYKNISVGCDSFCLDGFQGITAQKYSCSGCEECYLNWTGGICPFTSCLKGLLNGPCGG
                     ADKNGKCEVNRSRDCAWIKIYEKMKKLGIDKNLKENDIFIKNYLLMGKKKEFPL"
     gene            complement(2213..4204)
                     /gene="rsxC"
                     /locus_tag="PLW95_07670"
     CDS             complement(2213..4204)
                     /gene="rsxC"
                     /locus_tag="PLW95_07670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOV22532.1"
                     /translation="MEKEKIIIEYTNYKLKPENEIEELLKGTKSIYIFWCKKCFTKAT
                     LDEPFPEIIGKIKQEKKVDFSAVDFLCNNYHINKIIEDIDFSSFEVIGVISCGIGIQL
                     VSDIIDNKIVIALSDTISFSENSTNFPIEHGISLSPEKCGGCGQCYLNITGGICPIIN
                     CAKGLLNGPCGGADKNGMCEVNKNKKCAWIEIYERLKKQKEKLNPTIEIRNYNIIPDE
                     EKEKRTDENIERRQSGFYGGLYPEEEKEKTENIKIKKYQDPPYVIIFLSQHTGIPSIP
                     IVKVGDKVKVGQKIGSSSGFISSNIHSSISGKVLSIEEKIHPVSLTLEPAIVIENNGL
                     DDLSSEIQPLENWYNLPKEKILDFINEKGIVGLGGAMFPTHVKLSPPKKVDTLIVNGC
                     ECEPFLNSDNRVMIEYYREIIKGIEIVKKILEVKNVIVGIEDNKKVAINLLKQYSSEN
                     IKISQLPTKYPEGAEKILIKCLTGRQVPKKGLPFDVGVVVLNISTILAIYRAINYGFP
                     LISRVITISGEDIKMTGNYEIKIGFPLKHIIPSFIDENEISEYDIKMGGPMMGICQEN
                     FETAVIKGTTGYTFLKKTPVNFDEDRNCIKCGRCVDACPVELYPLYFALLGQEGKFLE
                     MGKYNVNECIECGCCQYICSSKINLLKFIKEGKKCLQQK"
     gene            complement(4786..5130)
                     /locus_tag="PLW95_07675"
     CDS             complement(4786..5130)
                     /locus_tag="PLW95_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014488412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22533.1"
                     /translation="MTYHKELADGKWFKLSLVEQLANIGSEVLRAISWEKKDKNYSKL
                     AAERGIELIDLTISDPKNRKRLKEICRLRELVCDYFFFDNEYRSDEKQWENYFCPFMV
                     YVRKKRYDVKEK"
     gene            complement(5117..5470)
                     /locus_tag="PLW95_07680"
     CDS             complement(5117..5470)
                     /locus_tag="PLW95_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020004846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5674 family protein"
                     /protein_id="HOV22534.1"
                     /translation="MKIVAENKIKLSELKKMAEESFGNLVKGVVDIEKKIMVIGGELH
                     SDEEAFLLENGSEQKNIWGINIYPEKIGDDFIEFDSIINLRPSFGNRTRGIESYEIRN
                     KIIEIVKGKVENDIP"
BASE COUNT         1679 a         1217 c          718 g         2168 t
ORIGIN      
        1 gttgctattt gaatacttac ttttgccctt tcttcctttt ttgcttctgc catcattttt
       61 atatattttt cgggaacact aacacccgca acattttcat tcatatattt tgccatacct
      121 gctgatttaa gaagaacaat tccacaaaga accggaactt tataattttc agtcattttt
      181 ataaattttt caaaaatact tatatcatag acggcctgtg tctgaaaaaa ctttgctcct
      241 gcctctattt ttttctccat ttttataact tgtaattcta ctggctcata acagggactt
      301 acaactgccc caagaaaaaa ttctggtttt ccatctaatt ggttacctac aatgtctttc
      361 ccttcaatta ggcaagatgc tgtctttaat aatgaaacag aatctaagtc aaaaactggt
      421 ctactatctg gatggtcacc taaggttgta tggtcacctg tcaagcataa aacatttctt
      481 atacctaaaa cataagcaga taaaaggtct gattgtaagg caagtcggtt tctatctctg
      541 catgtaatct gaaaaattgg ctcaaatccc ttttcaataa gtaaatgaga aacagcaagt
      601 gagccaagtc gcataacaga actttgtaag tcagtcacat ttaatgcatc tactttccct
      661 cttaaaactt caacttcttc aagaaatttt aaatttgttc cttttcccgg tccaacttca
      721 gaagttataa caaacttatt attagttaat ttttcttcaa gttcgcttac cattttttct
      781 cctttttctc tcctttggta ctgtcaaaat ttttgttacc tgttcatttc cccctcactc
      841 tcgcccctct cccctatggg gagaggatat aggtgagggg cgatttcatc tccttttcat
      901 ctattttttg acactactct ctcctcttaa taaaaatagc gaaattcaaa aatttcccct
      961 cgcgtgtagt ccgaggggac gaacctaacg ccattatgct tctcaaatcc cccttgaaat
     1021 ccccctttat acctgttgtg ccttttgaaa tacattacgg caccaactct cctttactaa
     1081 actttgctac cttagtcggt ttattaaagg gggaaatatc aactccccct catcccccac
     1141 ttctccccgg cggggagaag agatgaaatc cctcctaatc cccgttgtgc ctattgaaat
     1201 acattacggc accaactctg tgggctaaac tttgcaactt tagtcggttt attaaagggg
     1261 gaacgttgtc tccttccttt atcaaagctt gcccgtccat ctttccgcca aaaatagcag
     1321 ctgattcgcc attgtttctg gcgaatgtgg aggggaaggt atggatgtat tttaattata
     1381 aatccctctc tatctccctt tataaaaggg agaattaatc cccgttgtgc ttgctataaa
     1441 tctcccctca cccctgttgt gctatctaag aaacgataac gcaccaactc tgaggcacaa
     1501 ggtaagcaac ttcagtcggt ttgttaaagg ggagagtatt aaaatcccct ctaactcccc
     1561 ttttataaag ggaaaaatcc tgtttattaa agagggaatt ccttcttctt ccccattaac
     1621 aaataattct tgataaaaat atcgttttct ttcaaatttt tatctatacc caattttttc
     1681 atcttttcat aaatttttat ccatgcacaa tctcttgacc tatttacctc acattttcca
     1741 tttttatccg ctcctccaca gggtccattt aaaagtcctt ttaagcaaga agtaaaagga
     1801 catattccac ctgtccaatt caaataacat tcttcacatc cagaacaact atacttttgg
     1861 gcagttatcc cctgaaatcc atctaaacaa aatgaatcac atccgacact aatattttta
     1921 taaacttttt tatcctcaat taaaattttt gaaatcacct gaacaccaac accacaggag
     1981 aaaacaataa tattttcaca tttttcaaat tttttaatgt ttttattaac aattgtttct
     2041 gcaaactcct cattacaaag ataatctatt tttataattt caattatatt ctggttgttc
     2101 tttttcaaaa attcatttat tccttcttct ggaaaataaa cttctttaca accaaaacat
     2161 ttaaaaacaa ttactttttt ttcaagatat tttaaaattt catcttcatt ttttattttt
     2221 gttgcaagca ttttttccct tcttttataa atttaagcaa gtttattttt gatgaacaaa
     2281 tatactggca acaaccacac tcaatacatt cattaacatt atatttcccc atttctaaaa
     2341 attttccctc ctgccctaaa agagcaaagt aaagtggata taattctaca ggacaggcat
     2401 caacacatct accacatttt atacaatttc tatcttcatc aaaatttaca ggtgttttct
     2461 ttaaaaatgt ataaccagtt gttcctttta ttactgctgt ttcaaaattt tcctgacaaa
     2521 ttcccatcat tggaccaccc atttttatat catattcaga aatctcattt tcatcaataa
     2581 atgacggaat aatatgcttt aatggaaaac caatttttat ttcatagttt cctgtcattt
     2641 taatatcttc tcctgatatt gtaataacac gactgataag aggaaaccca taatttattg
     2701 ctctataaat tgcaagaatt gtgcttatat ttaaaacaac aacaccaaca tcaaaaggta
     2761 gccctttttt aggaacttgc ctaccagtta ggcattttat taaaattttt tctgcacctt
     2821 ctggatattt tgttggtaat tgtgaaattt ttatattttc agatgaatat tgctttaata
     2881 aatttattgc gaccttttta ttatcttcta ttccaacaat aacattttta acttcaagaa
     2941 tttttttaac aatttcaatt ccttttataa tttccctata atactcaatc ataaccctgt
     3001 tatctgaatt taaaaatggt tcacactcac atccattaac tattaaagta tctacctttt
     3061 ttggtgggga taattttaca tgtgttggaa acattgcccc acccaatcca acaattcctt
     3121 tttcatttat gaaatccaaa attttttctt ttggtaagtt ataccaattt tcaagtggtt
     3181 gaatttcaga agataagtca tctaaaccat tattttctat aactattgct ggttccaaag
     3241 ttaaacttac tggatgtatt ttttcttcaa ttgaaaggac tttaccactt atacttgaat
     3301 gaatattgga agaaataaaa ccagaagaag agccaatttt ctgcccaact tttactttat
     3361 ctccaacttt aacaattgga atagatggta ttcctgtgtg ttgagaaaga aatataatta
     3421 cataaggggg gtcttgatac tttttaattt ttatattttc tgttttctcc ttttcctctt
     3481 ctggatataa accaccataa aagccacttt gtcttctttc aatattttca tctgtccttt
     3541 tttctttttc ttcatctggt ataatattgt aatttctaat ttcaattgta ggatttaatt
     3601 tctctttttg tttttttaat ctttcatata tttcaatcca tgcacatttt ttattcttat
     3661 ttacttcaca cattccattt ttatccgctc ctccacatgg tccatttaaa agtccttttg
     3721 cacaatttat aataggacaa attccaccgg taatattgag ataacattgt ccacatccac
     3781 cacatttttc aggacttaaa ctaataccat gctcaatagg gaaattggta gaattttcac
     3841 taaaagaaat tgtatctgaa agggcaatca ctatcttgtt atcaataata tctgaaacaa
     3901 gttgtatccc aattccacag gaaataactc caattacttc aaaagaagaa aaatcaatgt
     3961 cttcaattat tttatttata tgataattgt tacataaaaa atctacagca gaaaaatcaa
     4021 cttttttctc ctgctttatc ttcccaatta tttcaggaaa gggctcgtca agtgtcgctt
     4081 ttgtaaaaca ttttttacac cagaaaatat aaatactttt tgtccctttc aaaagttctt
     4141 ctatttcatt ttctggtttt aatttataat ttgtgtactc aataattatt ttttcttttt
     4201 ccatttattt tccttgcttc tcatacaaat ttgacaccaa atttattgtc ttgttttaaa
     4261 tctctcctct tttctttctt ctctctcccc tgcggggaga gggcgggggt gagggggttt
     4321 tccccatttt ctttttccat ttatttcctt tgcttttcat acaaatttga caccaaattt
     4381 attgtcttgt tttaaatctc tcctcttttc tttcttctct ctcccctgcg gggagaggct
     4441 cgcccgtagg gtcctcgctt ttggctacgg atatagtaat ccacagaatt tctctgggct
     4501 atgcccgagg ggatggattt atatttaaaa caccaactct gccctgctaa tccccgctac
     4561 tttagtcggt ttgctaaagg gggaagtttt tctccttcct tttacaaagg aaggttggga
     4621 tagattttct gtaattactc gaaatcccct ctatctcccc tttattaaag gggagaatta
     4681 aaaggagaat taaacaccaa ctctgccctg ctaatccccg ctactttagt cggtttaaca
     4741 gagggaaaat tgtcagaatt tattaacttg acagtattaa atttttcatt tttcttttac
     4801 atcatatctt ttttttctaa catacaccat aaaagggcaa aaataatttt cccattgttt
     4861 ttcatctgac ctatactcat tatcaaaaaa gaaataatca catacaagtt ctctcaatct
     4921 acaaatttct ttcagtcgct ttctattttt ggggtcagaa attgtaaggt caataagttc
     4981 aattcctctt tcagcagcaa gttttgaata atttttatct tttttttccc atgaaattgc
     5041 ccttaaaact tcacttccaa tatttgccaa ctgttcaaca agagataatt taaaccattt
     5101 tccatctgca agttctttat ggtatgtcat tttccacttt tccctttact atttcaataa
     5161 ttttatttct tatttcataa ctttcaattc ctcttgttct atttccaaaa gaaggtctta
     5221 aatttataat agaatcaaat tcaataaaat catctcctat tttttctgga taaatattta
     5281 ttccccatat atttttttgt tctgacccat tttcaagcaa aaatgcttct tcatcactat
     5341 gtaattcccc gccaatcacc ataatttttt tttcaatgtc aacaacacct tttactaaat
     5401 ttccaaaact ttcttctgcc atttttttta attccgataa cttaatttta ttttctgcta
     5461 ctattttcat tttttccctc atcttaatcc ccgttgtgct atctgtaact ccctaaatcc
     5521 cctctaactc ccgttgtgct atctgggaga cgatgacgca ccaactctgc cctgctaccc
     5581 ttcgctactt cagtcggttt atgaaagggg agaatatact ccttcccttt ttaagggaag
     5641 gatgggatgg attttatcaa atccccctca tcccccttta taaaggggga aatgactcct
     5701 tcctttatca aaaggaaggt aggctcgccc gtaggggcta cgccctgagg ggatccgtag
     5761 ccaaaagcga ggaccctacg gg
//
LOCUS       DAOOVW010000070         5708 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_8013_length_5708_cov_9.031842, whole genome shotgun sequence.
ACCESSION   DAOOVW010000070 DAOOVW010000000
VERSION     DAOOVW010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5708
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8013_length_5708_cov_9.031842"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(412..1629)
                     /locus_tag="PLW95_07685"
     CDS             complement(412..1629)
                     /locus_tag="PLW95_07685"
                     /inference="COORDINATES: protein motif:HMM:NF039709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6263 family protein"
                     /protein_id="HOV22535.1"
                     /translation="MKKLLIFLSIFLITSQILISGEKEGDELLKKGIEALSQGKTENA
                     LEFFIKSTEEKANFAQGYYYIGLCYAKLGEIEKAKQNLIYAKVLTDENDFKNEITKVL
                     EDIENGKYEKVNVVKVDEKEEQEIKEEVKSEEQKETEEKEKVKLSYKFNKGDVSYYGL
                     NIKDKEKGKINNNPFQREQNIYIEFKEEVIDIDENGNGTTKLTLVRATYEGQDIGEKG
                     KEAYIKFTPNGKIIESQNLQEIIDQFIKVIRKSIAGIVPGFDRIPLNIDLKKFSSNEF
                     NAFWEVAKPILPEEEIKVGGSWETPPSGWQRDIGQKQPTKYTLKTKEGNNATIEIKME
                     QEENKINGEAVFDLEKGKVIKQNITVIIPKAKGSIDSKDIEKTGIMKGVPSFKMSYEI
                     EKQTTFNMNLLNK"
     gene            complement(1626..2231)
                     /locus_tag="PLW95_07690"
     CDS             complement(1626..2231)
                     /locus_tag="PLW95_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOV22536.1"
                     /translation="MPTGFTLIELLVVIAIIAILAAMLLPALSQARAKARQSVCINNL
                     KQIYLGFSMYAADYNEIIPPRDTGLAEEFPFCFINWPNFIRPYLEPNLNKIDIINWPP
                     PPNFYFCPESKKAKAKFLQEYPIFTPFTSYIIHTEPPDGDPIVKGKKIDGRWEDEEGN
                     YGASNIWLLEDPHFGDWADILHSGGINKLYLDGHCKWEKIR"
     gene            complement(2353..3252)
                     /locus_tag="PLW95_07695"
     CDS             complement(2353..3252)
                     /locus_tag="PLW95_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22537.1"
                     /translation="MRKFFISLLFLVIFNISLFAGDEIKISEIMKKIENNIENVKDGE
                     VKNFTGDIYFKLSFGKNTYMTIKPYMEKIFEREIPEEINIKGKFIGSFSTGKLDNFLI
                     KGNSEIGSFIFMTKGDNFKLLLPDLQIQIEDKISTVNEFAKQNKDIKKEIDKLKAPKI
                     DFYINKVKLKKGLDELNKMISNIEVKNKKEGDENLYAFDVDLGKINVLVYEKYYTIKE
                     ITFSNRSGNLIFSFPKPKNETNITLFNFLPEKVNLDIKNGNDILNLKLENLKYNRFLD
                     ENDFKIRNLNFPELLNYIYFKLF"
     gene            complement(3254..3667)
                     /locus_tag="PLW95_07700"
     CDS             complement(3254..3667)
                     /locus_tag="PLW95_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22538.1"
                     /translation="MEKNIDELLESLFKTEENPDIELDSLMKYSYFISNERKNSRSRL
                     KILTYICIFLLISNILLLKFYPIRKSLNSKNEEVKHIIISKNTKVETKYRNKTEKTAN
                     NEEIVLPLQNFFEEMDSFIEFAKSLENLQETENGG"
     gene            complement(3702..4226)
                     /locus_tag="PLW95_07705"
     CDS             complement(3702..4226)
                     /locus_tag="PLW95_07705"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOV22539.1"
                     /translation="MEINEEKQLVKEWKKGNKEALSTLFKKYLNKFSSLAAYLSNSKE
                     DAEDLLSETFIILLEKGNKFREKSSFFTYVYSIMTKLNLKKRRSKYYKIKLRQKLEKK
                     VKSDNNTEVEKILKLREIIEKLPQLYKEILFLRYNQDLKESEISEILKIPVGTVKSRL
                     SKAKKVLIKFWNKK"
     gene            complement(4619..>5708)
                     /locus_tag="PLW95_07710"
     rRNA            complement(4619..>5708)
                     /locus_tag="PLW95_07710"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1716 a          962 c          725 g         2305 t
ORIGIN      
        1 ttaataacta tgcagttaat accaatttga caacggacat agagtaaata ttttcttctc
       61 acccttaccc ctctccccat aggggagagg ctcgcccgta gggtcctcgc ttttggctac
      121 ggattatgaa ctacgcccga gggatataag tgaggagggg gatgttaatt tttattacac
      181 ctttgatgta aaaatagtat aatccccttt caaaatgtga attctttaaa ttttttcata
      241 gttataatat aaatacatct attattcaaa ttcacaattc taaatctaca ctactttttc
      301 tttttgataa ttttcgattc tccatagtta taagaaaacg ggattttggg ttaataaata
      361 gttattttaa ttggatagaa tcaaattaga aatttaaaat ttataaaaaa attatttatt
      421 taaaagattc atattaaatg ttgtttgttt ttctatttca taggacattt taaaagaagg
      481 aacacctttc ataattcctg ttttttcaat atcctttgag tcaatactac cttttgcctt
      541 aggaattatt actgttatat tttgctttat cacttttcct ttttctaaat caaaaactgc
      601 ctcgccattt attttatttt cttcctgttc catttttatc tcaatagttg cattatttcc
      661 ctcttttgtt tttaaggtgt attttgttgg ttgtttttgt ccaatatctc tttgccaacc
      721 acttggtggt gtttcccaac ttccaccaac ttttatttct tcttctggaa gaataggctt
      781 tgcgacttcc cagaaagcat taaattcatt agaagagaat tttttaaggt caatgtttaa
      841 agggattcta tcaaaaccag gtacaattcc tgcaatgctt tttctaataa cttttataaa
      901 ttggtcaatt atttcctgta aattctgact ttcaattatt tttccgttag gtgtaaattt
      961 tatatatgct tcttttcctt tttctccaat atcttgtcct tcataagttg ctcttactaa
     1021 tgttaattta gttgtcccat ttccattttc atcaatatca attacttctt ctttaaattc
     1081 tatataaata ttttgctctc tctggaaagg gttattgttt atttttcctt tttctttgtc
     1141 tttaatattc aatccataat aggaaacatc tcctttatta aacttgtaag aaagtttaac
     1201 tttctctttt tcttctgttt ctttttgttc ttcacttttt acttcttctt taatctcttg
     1261 ttcctctttc tcatcaactt ttactacatt tactttttca tatttcccat tttctatatc
     1321 ttcaagaact tttgttattt catttttaaa atcattctca tcagttaaaa cttttgcata
     1381 aattaaattc tgttttgctt tttcaatttc tcccaatttt gcataacata aaccaatata
     1441 ataataacct tgcgcaaaat ttgctttttc ttctgttgat ttaataaaaa attctaaggc
     1501 attctcagtt tttccttgac tcaatgcttc aattcccttt ttaagtagtt catccccttc
     1561 tttttctcct gatattaaaa tctgtgaagt tattagaaat atacttaaaa aaattaataa
     1621 ttttttcatc ttattttctc ccatttacaa tgtccatcta aataaagttt atttatacca
     1681 cctgaatgta aaatatctgc ccaatcgcca aaatgagggt cctctaataa ccatatattt
     1741 gatgccccat aattaccttc ttcatcttcc catcgcccat ctattttttt gcctttgact
     1801 attgggtctc catctggtgg ttctgtatgt ataatataac ttgtaaaagg tgtgaatata
     1861 ggatattctt gaagaaattt agcttttgct tttttacttt caggacaaaa ataaaaatta
     1921 ggaggaggtg gccaattgat aatatcaatt ttatttaagt ttggttctaa ataaggtctt
     1981 ataaaatttg gccagtttat aaaacaaaat ggaaattctt ctgccagacc tgtatctctt
     2041 gggggtataa tttcattata atcagcagca tacattgaaa agcccaaata tatttgcttt
     2101 aaattattta tacatacaga ctgtcttgct tttgcccttg cctgtgataa agcgggcaaa
     2161 agcattgctg ctaaaattgc tataattgca attacaacaa gcaattcaat taaagtaaat
     2221 cccgtaggca ttaacttagc acttctgcgc ctatatgcct gagcgttagc gaagatgggt
     2281 agggctggat ggtatatttc tctctttctg tttgtccaga acaactcatt tttcattata
     2341 tttccttttt atttagaaaa gtttaaaata tatatagttc aaaagttcag gaaaattcaa
     2401 atttcttatt ttaaaatcat tttcatcaag aaatctgttg tatttcaagt tttctaattt
     2461 caaatttaag atatcatttc cattttttat atcaaggttc actttttctg gtaagaaatt
     2521 aaatagggtt atgtttgttt cattttttgg ctttgggaaa gaaaaaatga gattacctga
     2581 acggtttgaa aatgtaattt cttttattgt atagtatttt tcatagacaa gaacattaat
     2641 ttttcccagg tcaacatcaa aggcataaag gttttcatct ccctcttttt tattttttac
     2701 ctcaatatta ctaatcattt tatttaattc gtcaagacct tttttcaatt ttactttgtt
     2761 tatataaaaa tcaatttttg gtgcctttaa tttatcaatt tcttttttta tgtctttatt
     2821 ttgttttgca aattcattta cagttgatat tttgtcttca atttgaattt gtaaatcggg
     2881 aagaagaagt ttaaaattat ctccttttgt catgaaaatg aaactaccaa tttcggaatt
     2941 acccttaatt aaaaaattat ctaattttcc tgttgaaaat gagccaataa atttcccctt
     3001 aatatttatt tcttctggta tttccctttc aaaaattttt tccatataag gttttattgt
     3061 catatatgtg tttttaccaa aagaaagttt aaaatatata tctccagtga aatttttaac
     3121 ttctccatct tttacatttt caatattatt ttcaattttt ttcataattt ctgatatttt
     3181 tatttcatct cctgcaaata aagaaatatt aaaaataact aaaaacaata aagaaataaa
     3241 aaattttctc attttatcct ccattttcag tttcttgtaa attttcaagt gattttgcaa
     3301 attcaataaa tgaatccatt tcttcaaaaa agttttgtaa aggcaataca atttcctcgt
     3361 tatttgctgt tttttccgtt ttatttctat attttgtctc tacctttgta tttttagata
     3421 ttattatgtg tttgacttct tcattcttag aattcaaact tttcctaatt ggataaaatt
     3481 ttagaagaag gatatttgaa attaataaaa atatacagat ataagtcaga atttttaatc
     3541 tgcttcttga gttttttctc tcattagaaa taaaataact gtatttcatt agtgaatcta
     3601 attcaatatc tgggttttct tctgttttaa acaacgattc taataattca tcaatattct
     3661 tttccattta attaattagt tccataaaaa ttaaatttgg ttcacttttt attccagaat
     3721 tttatcagga ctttttttgc ttttgaaaga cgagatttca cagttccaac tggaattttt
     3781 aaaatttcag agatttcact ctcttttaaa tcctgattat atcttaagaa taaaatttcc
     3841 ttataaagtt gaggaagttt ttcaataatt tctcttaatt ttaaaatttt ttcaacttcg
     3901 gtattattat ctgactttac ctttttttct aatttttgcc tcaattttat cttatagtat
     3961 ttacttctcc ttttttttag atttaattta gtcattatac tataaacata agtaaaaaaa
     4021 gatgattttt ctctaaattt atttcctttt tcaagaagta taataaatgt ttcagataaa
     4081 aggtcttcag catcttcttt ggaatttgat agatatgcag caagagaaga aaatttatta
     4141 agatattttt taaaaagcgt agatagtgct tctttatttc cttttttcca ttcttttacg
     4201 agttgttttt cctcatttat ctccattata aaatttttat catagatttg tgataaaggc
     4261 aaaagttaaa atatcgtaaa aactctatat ctttttgtat aatacaatta tctggataca
     4321 aagttatata gttccatgaa aaataatttt agttcacttt ttattttata attttatcaa
     4381 gatttttttt aaatgtgatt tataagttac agctgggatt tttaaaattt cagaaatctt
     4441 ggctcttttt aaaagttaag agtagataaa aacaattggt aaaaaacaca aacataaagt
     4501 tataagggaa acaaagaagg gaaaaaattc ggaataagtt ttgtatctta tacaaatcat
     4561 aaaaatacta taaataaaaa agggcagggg gattacccct gcccttaatt taaaatttat
     4621 ggtcaagccg ctcgacctat tagtacccct ccgcttaggc cattactgac cttacactcg
     4681 gggcctatca accaggttgt ctacctgggg tcttactctc catcttgcga tggagaaggg
     4741 aaacctaatc ttgaggtggg cttcccgttt agatgctttc agcggttatc ccttccggac
     4801 atggctaccc agcgattacc cttggcagga tagctggtac accagaggtc cgtccagccc
     4861 ggtcctctcg tactagggcc ggctcctctt caagtttcct acgcctgcgg cggatagaga
     4921 ccgaactgtc tcacgcatat ttatccactc ttttcagaat ggtatggact ataccttcat
     4981 cctttcctcg ctggaaaggc gactggcgta ttattctttc tttttattga aagaatctca
     5041 tcttttacag atgaagtctc tacgggggta agtaaattat tttctttcaa aaacttctct
     5101 actacttccg aagtaatagt tctattggta gaataattca gtattttgaa agaatctacc
     5161 atccgacaca attccaaaaa ggaatttgca tcagaaacct tagaaagttt atctataatc
     5221 ttcaatccca gtttaatatg ttgtttcttc aattgtacat acggttttaa ggtttgaaga
     5281 atacgattta cttccttagg ttgcacaatt gtataatcgc tgattcctgt tttgcgatgg
     5341 cgtatatatc cacttttcaa ttgtgtttta atccagtgca gaatctttta attattactc
     5401 ctctgataaa aactcacact gcatctgatt tggtatccaa aaatataatc ttttcttcta
     5461 accaattgaa aaaaaatact tccatcgcaa tccaaaaaac ctgcgatata agcgagaatt
     5521 gtgcggtcca taatttacca ccttccctcg gtattaccct ttcaaccagt tcatattgag
     5581 gagggctcca ccgatatgag ccaattttat ttttcctaaa gttaccttta ggcaacgcca
     5641 atgttgacgt tctgaaccca gctcacgtac cgctttaatg ggcgaacaga ccaacccttg
     5701 ggacctgc
//
LOCUS       DAOOVW010000071         5592 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_8213_length_5592_cov_7.408705, whole genome shotgun sequence.
ACCESSION   DAOOVW010000071 DAOOVW010000000
VERSION     DAOOVW010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8213_length_5592_cov_7.408705"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="PLW95_07715"
     CDS             <1..771
                     /locus_tag="PLW95_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOV22540.1"
                     /translation="PSFANAQAGRRRSAKLEHRGFTLIELLVVIAIIAILAAMLLPAL
                     SRARENARQSVCMSNLKQIGIAVMMYCQDYNEVLPSVNIGQNWYIKLLRYTNPKLSGV
                     PVAEYPAKPKGPVTVFVCPSNRYYYTSGSTTPWSYAVNYTMNMECGILFTGGPSNYPR
                     KYASIKKPSEKVIIGDSTYGPGTTGSGISYIGTYIWFQGGTAYVGTNNRWLGYIHNDG
                     SNLLWVDGHVSWQRKDIIEAQGLQYGVGSGGGWFDFDN"
     gene            831..1895
                     /locus_tag="PLW95_07720"
     CDS             831..1895
                     /locus_tag="PLW95_07720"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HOV22541.1"
                     /translation="MKRVIAYVVCIFFCIGISCLKGAENLLLIGDFEYDRDNNGVPDG
                     WAKRGVATSNVSYSMDTEVVHSGKKSLKMVFDSKRTESDEGYVVLRDKKMEFFERGRK
                     YRLTGYVKGKEFKGNAVLSVSIYGENNVLLLTSADKVGLSGETDWLLLKTEFTVPANA
                     IGVDVYTDTSGGIGGGIVWWDSISLYDITGEKEPEYAEKVIDSFENEESMKFWISSGA
                     LITRSDEYASDGKFSLKMDFKGSDTDTWPGVKRTLFFDKGEVDWTKYEYLAFDVYNPK
                     TNPEYISLRIDDAEKKASFVSYVCVPGTWTNIKVKISTLTGINTSMIESVLFYMRMPR
                     RDATLYLDNVRFLTIKEGEK"
     gene            1892..4261
                     /locus_tag="PLW95_07725"
     CDS             1892..4261
                     /locus_tag="PLW95_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HOV22542.1"
                     /translation="MREKIMVVLCFLFLRVLWSNSTEGRTIINEKFQSSLLDRGTWQV
                     TDEGVSIKDGKCLISEGGQILSVNSYPEGVLEIRLSLTKPLTSGAVAWGFRHKYGARP
                     RITFSVEESMELRASIYDNEGKKMIDSQKIPLDCKVHNYKIVWQPKRVVFYFDGKEIF
                     NYDFEEDIEISPRPITIYNVGSDTVVSVEEITYRTDATDEQLAEIKNKYQFRLGSIKD
                     LKFVELIHVDMTELPEVSDFWKERGYIIFSRPYTQRVFCDDTPEAGEIIDVNKGLTTF
                     VTPDEYEPITFAVHALKETKEYEVSITDLSDGKGHFIFADAISIGMVRSLNKRLLYNY
                     KWSPDYIFMPTFIEGKKRANIPSGKNAQYWLTIKVPKDTKAGKYFGMIEFASIYGPEG
                     GIPTRFPLTIEVLPFTLKEADRYTYGFWYPASNYINTREEFASDFLEMRKHNMNALFG
                     GVGIGATAEEKDGKIVIKGVSDKFNWICEEYKKADFKKPILYSGDIGSSWANSRVADI
                     QSEQWQEEYIRYTRAVLSMVKKNYGLDLIVQPIDEPAWQGEAIKDKCVRYLRLLKKAG
                     AVTETDGPYDTFMVEEAGPYSDVWNINGGLCPIEKIKQHKAEGKILWAYNNDVEGIRP
                     ELMRYAAGYWLWISGADGINNWAYRTRSGKSIYNDLSGETSNFVYYYPKTDNEIGGPA
                     IAFEGFREGIDDIRYLLTWEDAVEKAKKEGKTKLAGESQAKITEVFSKINWNYRLRDQ
                     AKWQRTFYLSTGQKAVVGDLKVTNGLSWEDYDKIRKVIADEIVKLISEK"
     gene            4608..>5592
                     /locus_tag="PLW95_07730"
     CDS             4608..>5592
                     /locus_tag="PLW95_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22543.1"
                     /translation="MKKRAEYIGGKWGVMVHFLSDFPCNRERVNLEIDEWNKIVDNFD
                     IKNFVRNLSKVKTNWLIFTIGQNSGFYCSPNKTYDEIVKREKSRLSKRDLIKELGEEL
                     NSKNIKLIAYLPSHAPSRDIYAVERLKCTPIWEDGGIRWGLKKEEYKFRAGVDERLSE
                     FQRNWENIIIEWSKRWGKLVSGWWIDGCYYSDKMYNFPEPPNFKSFADALRSGNKDSI
                     IAFNPGIKIPVISLTEYEDYTAGEIANGFPVFYKWVPFTRFINNAQFHILTFLGDIWG
                     LGEEPRFPKELIISYTKYITHLGGAITWEFPLSDMKGNINKKFLNQLISLSK"
BASE COUNT         2064 a          657 c         1241 g         1630 t
ORIGIN      
        1 ccctcctttg ctaatgctca ggcgggcagg cgcagaagtg ctaagttaga gcataggggg
       61 tttacattga tagagttgct tgtggtaata gcgattatag cgattttggc agcgatgtta
      121 ttacctgctt tatcaagggc aagagaaaat gcaagacagt cagtatgtat gagtaattta
      181 aagcagatcg gtatagccgt aatgatgtac tgccaggatt ataatgaggt attaccttct
      241 gttaatatag gacaaaactg gtacataaaa ttactaagat atacaaatcc aaaattatca
      301 ggtgtaccag tagcagagta tccagcaaag ccaaaaggac ccgtaaccgt atttgtttgt
      361 ccaagtaata gatactacta cacgtctggt agtactactc catggagtta tgctgttaat
      421 tacacaatga atatggagtg cggaatatta tttaccggtg gcccatctaa ttatccacgt
      481 aaatacgcaa gcattaagaa gccatcagaa aaagttataa ttggtgattc tacatatggt
      541 ccgggtacta caggatccgg aatatcttat ataggaacat atatttggtt tcaaggggga
      601 actgcttatg tcggtacgaa caatcgctgg ttaggatata ttcacaacga tggcagtaac
      661 ttactttggg tggatggaca tgtaagttgg cagagaaaag atattataga ggcacaagga
      721 ttgcaatacg gcgtgggtag tggcggaggg tggtttgatt ttgataattg atatgtggac
      781 aagaaataag gataattttg gcagaaccgg taagaaaaca aggaggaata atgaaaagag
      841 taatagcata tgtggtatgt atattttttt gtataggaat aagttgtttg aaaggggcag
      901 agaatttatt gttaattggg gattttgaat atgatagaga taataatgga gttccagatg
      961 gatgggcaaa acggggagtt gcaacatcta atgtgtctta ttcaatggat acagaagttg
     1021 tgcatagtgg gaaaaagagt ttaaagatgg tatttgatag caaaaggaca gagtcagatg
     1081 aaggatatgt ggtattacga gataaaaaga tggaattttt tgagagaggc aggaaataca
     1141 gattaacagg atatgtaaag ggaaaagaat ttaaagggaa tgcggtactt tctgtttcaa
     1201 tatatggaga aaacaatgtt cttctattga caagtgcgga taaagttggt ttatcaggag
     1261 agacagattg gttattatta aagacagaat ttacagtacc agcaaatgct attggtgtag
     1321 atgtatatac tgacacttcg ggaggtattg gaggagggat agtatggtgg gatagtatct
     1381 ccctttatga cataacaggg gagaaagaac cggaatatgc ggaaaaagta attgactcat
     1441 ttgagaatga agaaagtatg aaattctgga tttcttctgg tgcattaatt acaaggagtg
     1501 acgagtatgc ttcagatggg aagttttctt taaaaatgga tttcaaaggg tcagatacgg
     1561 atacgtggcc gggggtaaag cgaactttat tttttgataa aggagaagtt gattggacaa
     1621 aatacgagta tttagcattt gatgtttata atccaaaaac aaatcctgaa tatatttctt
     1681 taaggataga tgatgcagaa aaaaaggcga gttttgtaag ttatgtctgt gtgccgggta
     1741 catggacaaa tataaaggta aaaatatcca ctttaacagg aattaatact tcaatgatag
     1801 agagtgtatt attttatatg aggatgcctc gtagagatgc aactttatat cttgacaatg
     1861 tcaggttttt aacaataaaa gagggggaga aatgagagag aagataatgg ttgtattatg
     1921 ttttttgttt ttacgggttt tatggagtaa tagcacagaa gggcgaacaa ttatcaatga
     1981 gaaatttcaa agttccttac ttgaccgtgg gacatggcag gtaaccgatg aaggtgtatc
     2041 aataaaagat ggtaagtgtt taatttcaga aggagggcaa attctttcag ttaattcata
     2101 tccagaggga gtacttgaaa taagactatc tttaacaaaa ccacttactt ctggggcagt
     2161 agcgtggggt tttcggcaca aatatggagc aagaccccgt ataacttttt cagtagaaga
     2221 gagtatggaa ttaagagcaa gtatatatga taatgaaggt aaaaagatga tagacagtca
     2281 aaaaattcca ttagattgta aagttcacaa ttacaaaatt gtctggcaac ccaaaagagt
     2341 agttttttat tttgatggta aagaaatttt caactatgat tttgaagaag atattgaaat
     2401 atcccctcgt ccaataacaa tttataatgt tggttcagac acagtagtat cagtagaaga
     2461 aattacttat agaacagatg caacagatga acaacttgcc gaaataaaaa acaagtacca
     2521 atttcgtctt ggtagtataa aagacctgaa atttgtagag ttaatacatg tggatatgac
     2581 agaactcccg gaagtatcag atttctggaa agaaagggga tatataattt tttctcgtcc
     2641 ttacactcag cgagtatttt gtgatgatac accagaagca ggagagataa tagatgttaa
     2701 taaagggttg acaacttttg taacaccaga tgagtatgaa ccaataacat ttgcagttca
     2761 tgcactaaaa gagacaaagg aatatgaggt ttcaataaca gacctttcag atggaaaagg
     2821 gcattttatt tttgctgatg caatttccat agggatggtg cgttcgctta acaaacgcct
     2881 tctttacaac tataaatgga gtcctgacta tattttcatg cctactttta tagaagggaa
     2941 gaaaagagcg aatattccct caggtaaaaa tgctcaatac tggttaacta taaaagttcc
     3001 aaaggataca aaggcaggga aatattttgg tatgatagaa tttgcctcta tatatggtcc
     3061 agagggagga attccaacac gttttccttt gacaatagag gtattaccat ttactttaaa
     3121 agaagcagat aggtacacat atggtttctg gtatccagca agtaattata taaacacaag
     3181 agaggaattt gcatctgatt ttttagaaat gagaaagcat aatatgaatg cattatttgg
     3241 aggagttggg attggtgcaa ctgctgaaga aaaagatgga aaaattgtta ttaaaggagt
     3301 atctgataaa tttaactgga tatgtgaaga atataaaaaa gcggacttta aaaagcccat
     3361 tctttattct ggcgatattg gttctagttg ggcgaattct cgtgtagcag atatacaaag
     3421 tgaacaatgg caagaagagt atataaggta tactcgtgca gttctttcaa tggtaaagaa
     3481 aaattatgga ttagacctta tagttcagcc aatagacgaa ccggcctggc aaggagaggc
     3541 aattaaagat aaatgtgtaa gatatttaag acttttaaag aaagcgggcg ctgtaaccga
     3601 gaccgatggc ccgtatgata cctttatggt tgaagaagca ggaccttata gtgatgtatg
     3661 gaatatcaat ggtggattat gtcctattga gaaaataaaa caacacaaag cggagggaaa
     3721 aatactttgg gcatataaca atgatgtaga aggtattaga ccagaactaa tgagatatgc
     3781 agctgggtat tggctatgga tttcaggtgc tgatggtata aacaattggg cttatagaac
     3841 aaggagcggg aagagtatat ataatgattt aagtggagaa acatcaaatt ttgtttatta
     3901 ttatcccaaa acggacaatg agataggagg accagcaatt gcatttgaag gttttagaga
     3961 aggtatagat gatatacgat atcttctcac atgggaagat gcagtggaaa aggcaaaaaa
     4021 ggaaggaaaa acaaaacttg caggggaaag tcaagcaaaa attacagaag ttttttcaaa
     4081 aattaattgg aactatagat taagagacca ggcaaagtgg cagagaacat tttatttgag
     4141 cacaggccag aaagcagttg ttggggattt aaaagtgaca aatggtcttt cctgggaaga
     4201 ttatgataaa attcgtaaag ttattgcaga tgaaattgta aagttgataa gtgagaaata
     4261 aaaaagtggg ttggctaaaa aattaatgaa aattctctat ctatagagaa gaggttataa
     4321 gttatgtatt gtttatattc gaaggtgctt tgaaagtaat ttatattata ggaggtattt
     4381 ctcattacaa agaatagagt aaactttata atatctttaa aatgaagaat ataaaaaaat
     4441 taaatgtaaa agggtggtaa aattttaaag aaaaaaataa aaaaagaaaa agttggcatt
     4501 ttatcattaa gataaaagaa gaattattgc caaatagtaa ctactaaata tttaatctca
     4561 cacaaaattt ttttttgctg aagaagttta agagagaaaa agttaagatg aaaaaaagag
     4621 cagaatatat tggtggaaaa tggggcgtaa tggttcattt tttatctgat tttccatgta
     4681 atagggaaag agtaaattta gaaattgatg agtggaataa aattgttgat aattttgata
     4741 ttaaaaattt tgtaaggaac ttatcaaaag taaagacaaa ttggttaata tttaccattg
     4801 gacagaattc tggtttttat tgttccccaa ataaaactta tgatgaaata gtgaaaaggg
     4861 aaaaaagtag attatctaag agagatttaa taaaagaatt aggagaagaa ttaaattcaa
     4921 aaaatataaa acttattgca tatctacctt cacatgctcc atcaagagat atttacgcag
     4981 ttgaaaggtt aaaatgtaca cccatctggg aagatggagg aataagatgg gggttgaaga
     5041 aagaagaata taaattccgg gcaggagttg atgagcgact tagtgaattt cagagaaatt
     5101 gggaaaatat aataatagag tggtcaaaaa gatggggaaa acttgtaagt ggatggtgga
     5161 tagatggatg ttattattca gataaaatgt ataatttccc agaaccccca aattttaaaa
     5221 gttttgcaga tgcactaaga agtgggaaca aagatagcat aattgctttt aatcctggta
     5281 taaaaattcc tgtaataagt ttaactgaat acgaagatta tacagcagga gaaattgcaa
     5341 atggatttcc tgttttttat aaatgggtac cttttacaag atttataaat aatgctcaat
     5401 ttcatatttt aactttttta ggagatattt ggggattagg agaagaacca agatttccaa
     5461 aagaattaat tatctcatac acaaaatata taactcatct tggtggagca attacttggg
     5521 aatttcccct atctgatatg aaaggcaata taaataaaaa atttttaaat caacttattt
     5581 ctctttcaaa at
//
LOCUS       DAOOVW010000072         5410 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_8536_length_5410_cov_8.597946, whole genome shotgun sequence.
ACCESSION   DAOOVW010000072 DAOOVW010000000
VERSION     DAOOVW010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5410
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8536_length_5410_cov_8.597946"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(730..2733)
                     /gene="uvrB"
                     /locus_tag="PLW95_07735"
     CDS             complement(730..2733)
                     /gene="uvrB"
                     /locus_tag="PLW95_07735"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOV22544.1"
                     /translation="MDNFKLISKFKPAGSQPEAIKQLVKNLEKGKKQQILLGITGSGK
                     TFTMANVIEKVNRPVLVISHNKTLAAQLYSEFREFFPENKVRYFVSYYDYYQPEAYIP
                     ETDTYIEKDASINEDIDRLRLAATSAILSGRDVIVVASVSCIYPIGSPEDHKAMMLHL
                     EKGQNTERKYILKRLIEIQYERNEFDFTRGKFRVRGNTIEIIPSYEEIGVRIGMVGNK
                     IVKIEKFDPLTNKKLSDEEEIYIYPAKHFVTPRDKLTKAIESIKEELEICVDDFKKRG
                     KLLEAERLEMRTKYDLEMLEELGYCHGIENYSRHLAGRLPGERPEVLLDYFPDDYLIF
                     IDESHVTVPQIKGMYFGDKARKETLVQYGFRLPSALDNRPLKFDEFTKLANSVIYVSA
                     TPGKYELEKCGVNRLGEKSPLIVEQIIRPTGLIDPPIIVKETKTQIDDLIPRIKERIK
                     KKQRVLVITISKHLAEEIADYLKEFDIKVMYLHYEIDTLERVEILKDLREGNFDVLVG
                     INLLREGLDLPEVSLVAILDADKEGFLRSETSLIQIAGRAARSIDGEVVMYADRITDA
                     MRGAIEETERRRKIQIEYNKKYNITPRSIEKPIYKSVSEILGGKNIREERKIAEKEEE
                     YYGKDVFQTIKRLEKKMMGAAKKLDFEQAIYYRDKIKKLKEEK"
     gene            complement(3171..4868)
                     /locus_tag="PLW95_07740"
     CDS             complement(3171..4868)
                     /locus_tag="PLW95_07740"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HOV22545.1"
                     /translation="MNEGELEFEETKEDQPTEILPKERRVYSDKPDRSIFELYRQYQK
                     GNLELRPEFQRLQVWDNTKSSRLIESVLLEVPIPVIYLSEESDSKYSVIDGQQRLNAF
                     IKFLENNLRLSGLTILTELNGKRFQNIPKNLQDKFENATIRIIEIRKESNPDVKFEIF
                     ERLNTGAVQLNAQELRNCIYRGNYNDLLKELAEDKDFQFLLGLKEPHYRMQDRELILR
                     FFAFFHTTHLKYTPSMKHFLNKEMEKYRNLNNAEEKELRTVFKKSVGLSKSVFGDKAY
                     RRFVSGSSKDPNGYWETRKINKGLFDIVMFGFAMYEENQIVPNSDQIREKLIWLMTHD
                     LEFIDTITVTTDRKDKIHTRFDKWLSTLKEIVGTAKTESRTFSLQYKKQLWESNPTCA
                     ICGQRIHLVDDAEVDHVEQYWQGGKTIPANARLTHRYCNRSRPREDIQPTVGGKANAI
                     SPTTMVPKGIKYSRVISKGERTPRQAFRIPILEALIELGGTGEMDKILEKVEIKMKHI
                     LNSVDYKKLPSGVMIRWQNTAQWERYVMVQDGLLSPHSPRGIWEITEKGKEFLKNNR"
     gene            5222..>5410
                     /locus_tag="PLW95_07745"
     CDS             5222..>5410
                     /locus_tag="PLW95_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22546.1"
                     /translation="MSNLSIFLIILPQVFVNSFQSIVIPVFYHLPLSIDRKKIGGKII
                     TYYKTDWSVIMNKKKLILT"
BASE COUNT         1516 a         1030 c          796 g         2068 t
ORIGIN      
        1 tgtggtatat ttttcaaaat ccactctgtt cctttctctc ccttcctttg taaagcatgt
       61 ccgtcaatgt ctttgtggag gggaaggtta ggctcgcccg taggactacg cccgagggat
      121 ggatttctct atatctcctt attaaacccc gtctgctcaa cttatctcta ccttgcctgc
      181 ccgtctgagc attagcgaag gagggtaggg ctggttcgta tgctatattt taaactacct
      241 gtgtagaaca atcctcaaaa tccccgttgt gctatctggg agacgatgac gcaccaactc
      301 tgaggcacaa ggtaagcaac tttagtcggt ttacaaaagg gggaagtatt gtagaaacaa
      361 gcaccaactc tgctttgcta cccctcgctt ctttagtcgg tttaataaag gggaaaatac
      421 ttgccccctc atcctttcct tctccccgtt ggggagaagg aaatatccct ctcttcttct
      481 cccctctccc cacaggggat aggctcgccc gtagggctac gcccgagggg aagggtgagg
      541 gggatattaa tttttagtat acatttgatg taaaaatagt atgaatatct tagtgtccaa
      601 aaatatggga atttaggata atatttatgc tgttaaaata ataaaaaggg gaaaaagaag
      661 aaatgaaaac atactaatag aaataattaa atatcttaaa ttatagtttt acatttgaaa
      721 ggtttaagtt tatttttctt cttttagttt ttttatttta tccctgtaat atattgcctg
      781 ctcaaaatcc aatttttttg ctgctcccat catctttttt tcaagtcgtt ttattgtctg
      841 aaaaacatcc tttccgtaat attcttcttc tttttctgct atttttcttt cttctcttat
      901 atttttccct ccaagaatct cacttacact tttatagatt ggtttttcaa ttgaacgagg
      961 tgttatgtta tattttttat tgtattcaat ctgtattttc cttctccttt ctgtttcttc
     1021 aattgctcct ctcattgcat ctgttattct atcagcatac attacaacct ctccatcaat
     1081 acttcttgcg gctcttcctg caatttgaat taaagatgtc tcacttctta aaaacccttc
     1141 tttatcagca tcaagtattg ctacaagaga aacttcaggt aggtcaagtc cttcccttaa
     1201 taaatttatt ccaacaagaa catcaaaatt cccctctctt aaatccttta aaatttccac
     1261 tctttcaagc gtatcaattt cataatgtaa atacataact tttatatcaa attcttttaa
     1321 gtaatcagca atttcctctg ctaaatgttt acttatagtt attacaagaa ctctttgttt
     1381 tttctttatt ctttctttta ttcttggaat taggtcgtct atctgtgttt ttgtctcttt
     1441 tacaattatt ggcgggtcaa taagtcctgt tggtcttata atttgttcaa caattagagg
     1501 tgatttttct ccaagtctat ttactccaca tttttcaagt tcatattttc caggtgttgc
     1561 cgagacataa attacactat tggcaagttt tgtaaattca tcaaatttta agggtctgtt
     1621 atcaagtgca gaaggaagac gaaacccata ttggacaagt gtttccttcc ttgctttatc
     1681 tccaaaatac attcctttta tttgggggac ggtaacatga gattcgtcaa taaaaattaa
     1741 atagtcatca ggaaaatagt caagaagaac ttcaggcctt tctcctggta atcttcctgc
     1801 taaatgacgg ctataatttt ctattccatg acaataacct aattcttcaa gcatttcaag
     1861 gtcatatttt gttctcattt caagtcgttc tgcttcaaga agttttcctc tttttttaaa
     1921 gtcatcaaca catatttcta attcctcttt tatactttct attgcttttg tgagtttatc
     1981 tcttggtgta acaaaatgtt ttgctggata aatataaatt tcttcttcat cagataattt
     2041 tttgtttgtt aatgggtcaa atttttcaat tttaacaatt ttgtttccta ccatgccaat
     2101 tcttactcca atttcttcat aagatggtat tatttcaatt gtatttcctc ttactctaaa
     2161 cttacctctt gtgaagtcaa actcatttct ttcatactga atttcaataa gtcgtttcaa
     2221 aatatatttt ctttctgtgt tttgcccttt ttctaaatga agcatcattg ctttatggtc
     2281 ttcgggactg ccaattgggt aaatacagga aacacttgca acaactatga catctctacc
     2341 tgataaaatt gcacttgtag cagcaagccg taatctatct atatcctcat ttattgaagc
     2401 gtctttttca atataagtat ctgtttcagg aatatatgct tctggctgat aatagtcata
     2461 ataactaaca aaatacctta ccttattttc ggggaaaaac tctctaaatt cactataaag
     2521 ttgggcagca agtgttttgt tatgagaaat tacaagaaca gggcgattaa ctttttctat
     2581 tacatttgcc attgtaaatg tttttccact acctgtaatc ccaagtaata tctgttgttt
     2641 tttacctttt tctaaatttt taacaagttg ttttattgct tctggttgac ttcccgcagg
     2701 tttaaatttt gatatgagtt tgaaattatc cataaaaata ttatacctga aaaatccctg
     2761 tgattagtaa attatttatt taccattatc acactgtttc ttaaacagaa tccactctat
     2821 tttaacaaca aaacggttat ttttgcacct ttctttactt tttattttat cgggaggtgc
     2881 tattttaatt ctgatttttc gagtagattt ctcttttgtc ttgtctaaca taccttttcg
     2941 tgtagattta ttatgtctca attcgaaaat ttgacaattt ataaatgtat gttttacaat
     3001 ttttgtagtt aaaattttat ggatggttgc ttcttgtttt tacaggaaaa aatagacatt
     3061 atccacgtat tgctaaataa gaacataagt ttttcaactg tttaatttca gcttgggtga
     3121 gtgctgtaag gcacgaacct gccgtgttat acgatgttgg ttgcctttgg tcatcgatta
     3181 tttttcaaga attctttccc cttctctgta atttcccata ttcctcttgg ggaatggggg
     3241 cttaataatc catcctgaac cattacatac ctctcccatt gagcagtatt ttgccatctt
     3301 atcataactc cagatggtaa ttttttatag tcaacagaat ttagaatatg tttcatttta
     3361 atctcgacct tttctaagat tttatccatt tcacctgtac caccgagttc tatcagtgct
     3421 tctaatatag gaatgcgaaa agcttgacga ggggttctct ctcctttaga tattactctg
     3481 gagtatttaa ttccttttgg gaccatagta gtaggactaa tagcatttgc ctttcccccg
     3541 acagtaggtt gtatatcttc tcgtggtcta cttctattac aataacgatg tgttaatcgt
     3601 gcatttgcgg gtattgtttt tccaccttgc caatattgct ctacatggtc aacctcagcg
     3661 tcatcaacaa gatgaatcct ctgtccacag atggcacagg taggattaga ttcccaaagc
     3721 tgttttttgt attgcaaaga aaaagttcgt gattctgttt ttgctgttcc tacaatttct
     3781 ttaagtgtag aaagccattt gtcaaagcga gtatgaattt tatcctttct gtcagttgta
     3841 acggtgattg tatcaataaa ttccaaatca tgagtcataa gccatatcaa tttttcccta
     3901 atttggtcac tgtttggaac aatttgattt tcttcataca tggcaaagcc gaacattaca
     3961 atgtcaaaca aacccttatt tatttttctt gtttcccaat aaccattagg gtctttactt
     4021 gaaccagata caaatcttct atatgctttg tctccaaaaa cactttttga taatccaaca
     4081 ctctttttaa aaacagttct gagttctttt tcttcagcat tgttcagatt tctatatttc
     4141 tccatttctt tgttcaaaaa gtgtttcatg gaaggagtat atttaagatg tgtagtgtgg
     4201 aagaaggcaa agaagcgtaa aatcaactcc ctatcttgca tacgataatg tggttcttta
     4261 agccccaata aaaattggaa atctttatct tcggctaact cttttaacaa atcattatag
     4321 tttccccgat aaatacagtt tcgcaattct tgtgcattaa gttgaacggc tcctgtattt
     4381 aatcgttcaa atatctcaaa ttttacatct ggattggact cctttctaat ttctataatt
     4441 ctgattgtgg cattttcaaa tttatcctga aggtttttgg gaatgttttg aaatcttttt
     4501 ccattcagtt ctgttaaaat cgttagtcca cttagtctca agttattttc aaggaatttg
     4561 ataaaagcat tcaatctctg ctgcccatct ataactgaat atttactatc tgattcctcg
     4621 cttaaataaa taacaggtat tggaacttct aataataccg attcaattaa acgacttgat
     4681 ttggtattat cccaaacctg aagcctttga aactctggcc gtagttccaa attgcccttt
     4741 tgatattgac gataaagttc aaaaatactt ctatcaggtt tatctgaata tacccgccgt
     4801 tctttcggta gtatttctgt tggttgatct tctttcgttt cttcaaattc aagttctcct
     4861 tcgttcatat tctaacactc ctctttaagc aaccaatggg taaataggga cagcgaccat
     4921 ttaaactcct ttcgcttcca gtgttcttgc ccacattaac ccttgtcagg ttcgtaatgt
     4981 attagcttca aaaagtcaag tttatttttc cagtgtagca catctttaat tttgtgtcaa
     5041 aagaagaacc tgcctgaact acataattca gttccagcaa tcaaataaaa aggtaagtat
     5101 tcagtgaacc ccatcccgat aattcgggcc gttttctgtc tttgcacggt gctaaaaaca
     5161 gaacgggatt tactttcaac aaatcaactt cccttttaac tttatcacat acatctataa
     5221 attgtcaaat ttatctattt ttttaattat actaccccaa gtttttgtca atagttttca
     5281 aagtattgtt attcctgtat tttatcattt accattatct attgacagga aaaaaattgg
     5341 gggtaaaata attacttatt ataaaactga ctggtcagtc ataatgaata aaaaaaaatt
     5401 aatattaaca
//
LOCUS       DAOOVW010000073         5240 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_8885_length_5240_cov_6.746962, whole genome shotgun sequence.
ACCESSION   DAOOVW010000073 DAOOVW010000000
VERSION     DAOOVW010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5240
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8885_length_5240_cov_6.746962"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PLW95_07750"
     CDS             complement(<1..290)
                     /locus_tag="PLW95_07750"
                     /inference="COORDINATES: protein motif:HMM:NF024172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOV22547.1"
                     /translation="MGQKRKEVKSKRERGTRNQPVFGILCRDGRVWARIIDGVEKKDL
                     QPLIENKVKKGTVVCSDTWRGYTGIATKGYLHRLVNHSEGEYVNKEGNHINGL"
     gene            complement(295..444)
                     /locus_tag="PLW95_07755"
     CDS             complement(295..444)
                     /locus_tag="PLW95_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22548.1"
                     /translation="MIKEQTGLSEYKILKCCNKIREIMKRDIPKSFEGIVEVDETYFG
                     RQKEE"
     gene            complement(751..2109)
                     /locus_tag="PLW95_07760"
     CDS             complement(751..2109)
                     /locus_tag="PLW95_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22549.1"
                     /translation="MRRIVCFFILIIITVFSVEGGITIQIEKLADDFTIGKGQYLAPF
                     GLKVCGTSGEKISGFSLVFSNLEREDIEEIDVFKASGEYNKFLINFPQDWQKIDGEIN
                     TNEFPEIQVNFKEDLELPVFFENRIEFLIGFKLKETIKGPLTFSVTLTSISFDKSNKF
                     FPFSLKADILVDDKPPEIKVNTEKLYFSDNLTGIRINVETDEPLAFPAQIKPRFGWDA
                     KKKGIQWQIRDYNKWRPEAEQVSSRISMNKVKDNFYQYDYLFLIPLSENDKDIKEKTI
                     SYSLDPYPDQWGARKDDTGYDLIDGDARPGWFCISEVRWDIGSDPTIITFDLKDNVLI
                     GRVIVYCWENIDRIEIWGKKEEDENWQKMTSLPGISFPLYQYYIEPHLNLYVAEANFS
                     QLQQIRYLKILLFGEGWKTVTEIEIWGFPQSGTVIIPNGFNIYAIDKVGNTSKYNLKI
                     EK"
     gene            complement(2106..4991)
                     /locus_tag="PLW95_07765"
     CDS             complement(2106..4991)
                     /locus_tag="PLW95_07765"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HOV22550.1"
                     /translation="MNKKYFKKVVNMFIFYLFLNYPVISEEIPRIRIPFFQDNVIIDG
                     NIEEDVWKKAVSTGPFYLLGGKEIPTQESEAVIWHNGKRIFVAFICKEKDIDKLEAKV
                     IENDKQVWNDDCVELFVSPFSDADPYYHFVANTLGIKYDEKRIEEGSIDIPEWGGSWE
                     VQSRVFSDKYIVEMSIPFGLFDNFLSSDWRISFSRENKINGELSVWPPMGKERGFHNP
                     EKFAFLEGVNAQISSVIKVENAKFLFLSRENGLINGNLIIDFSNQGDGKTYILNFLLS
                     GVDNFYHQEQKEIFFSSGKTELNFPISLIKEGEYKIKCELKEGDEIVYFWQDEVEVPP
                     LSFSVSLLKPYYRNSIFPSQKISSIIIKIENLSSNSLLKGTIKIKGKDNKEIVQKSFS
                     FKGKEKEISIPIPDLKVGEYFIQIKSDKGEMVIPLKKLSPTEGTEVWIDENNNFVVKG
                     KPVFPLGFYSVSSCVNYKKSGYNFIFSYSLPSKETLEWLEENQAWEFASTIGLFTHAR
                     SSQLNQAGIQKVQDLISQNPYRIVGWYCADEPEIHKDLNRSAYRDALKLMRDMDPYRP
                     ISVVHDTTGGIKLDKDLTDIIMMDPYLGFEKNKNFPMRPLERISIFIDEAVKSCGNKK
                     PVWAVLQCFPSGYFGGDPEVSRYPTIQEIRAMTYLSLIHGAKGIIYWAFSSSSSNLLD
                     VPALWSALKEFSSEIHYLEPVLLSSYEVNWSKIKSVHYLKKELNGVEYIFAVNTRNTR
                     DQIDIPVDKNTYWVLSENRQVKGDGNKITDEFAPYQTHIYISQPVPDLPFRKTLENFR
                     EEDAWIGVSGSENLALGWEGGKIKVSSCNPYRCSFAAIDGGYISDWLSYNDNSPYVEI
                     NLRKKSNIKRIRLVFSPQYLNKISYNVKYFSDNNWHLINLKEPKIYHLFWDDEINKWV
                     EFSPEQKGISLCQAVVHEYEIDIPETEAFRIEFSSFGHLAEFGAYEK"
BASE COUNT         1619 a         1022 c          593 g         2006 t
ORIGIN      
        1 agtccattta tgtgatttcc ttctttattt acatattccc cttcactatg atttaccaat
       61 ctatgtaaat atccttttgt cgctatccct gtataacctc gccaagtatc tgaacaaaca
      121 accgtccctt ttttaacttt attctcaata agcggctgta aatctttctt ttctactcca
      181 tctattattc ttgcccatac tctcccatct ctgcaaagaa tcccaaaaac tggctgattc
      241 ctagttcctc tctctctttt gctttttact tctttcctct tttgtcccat atttttattc
      301 ttccttctgt cttccaaaat atgtctcatc tacttctact atcccttcaa aactctttgg
      361 aatatctctt ttcataattt cccttatttt attacaacat tttaaaattt tatactctga
      421 taatcccgtt tgctctttta tcatttttac ccttccagat actaaaaacc acttcaaaaa
      481 tttcttccat tcatatctgt ttaaatttaa cagatgcttc ttttttctaa actcaacttc
      541 accccttcaa gacaggaaaa ccataagtgt cagaagtcag gaaagatatt ggtatgtagc
      601 accttaggta attacaaaaa tttcttgatt tttctgctga tttattagac cttatatggt
      661 aaggatttcc ttgtttttga accttttgat gctaatatat tactggattt tatagtacac
      721 taatttatca caaaatcttt ttgggatata ttatttttct attttcaaat tatatttgct
      781 tgtatttcct actttatcaa tcgcatagat attaaaccca tttggaataa taacagtgcc
      841 agattgagga aaaccccaga tttctatttc tgtaactgtt ttccatcctt ctccaaaaag
      901 aagaattttt aaataccgaa tttgttgtaa ttgagaaaaa tttgcttctg caacatataa
      961 gttcaaatgt ggctctatat aatattgata taacggaaat gatattcctg gtaaagaagt
     1021 cattttttgc caattttcat cctcttcctt tttaccccaa atttcaattc tatctatatt
     1081 ttcccaacaa tatacaatta ccctaccaat taaaacattg tctttaaggt caaaagtaat
     1141 tatagtaggg tcagagccaa tatcccatct tacttctgat atacaaaacc aaccaggacg
     1201 agcatctcca tctataaggt cataaccagt gtcatctttt cttgcccccc attggtcagg
     1261 ataagggtca agagaataac taattgtttt ttcttttata tctttatcat tttctgaaag
     1321 aggaataaga aataggtaat catattggta aaaattgtct tttactttat tcatagaaat
     1381 acgactactt acttgttctg cttctggtcg ccatttatta taatctctta tctgccattg
     1441 aattcctttc tttttcgcat cccatccaaa acgaggtttt atttgagcag gaaatgctaa
     1501 tggttcatct gtctcaacat ttattctaat tcctgtaaga ttgtcagaaa aataaagttt
     1561 ttctgtattg acttttattt ctggtggttt atcatcaact aaaatatctg cttttaaaga
     1621 aaacgggaaa aacttattac ttttatcaaa tgaaatagaa gtaagagtta cagaaaaagt
     1681 taaaggacct tttatggttt cttttagttt aaaaccaata agaaattcaa ttctattctc
     1741 aaaaaaaaca ggtaattcta aatcttcttt gaaatttact tgtatttccg gaaattcatt
     1801 tgtgtttatt tccccatcta ttttctgcca gtcctgtgga aaatttatta aaaatttatt
     1861 atattctcct gatgccttaa aaacatctat ctcctctata tcttctctct ctaaatttga
     1921 aaaaactaaa ctaaatccac ttatcttttc accagaagtg ccacatacct ttaatccaaa
     1981 tggggcaaga tattgtcctt tcccaatagt aaaatcatct gccagtttct ctatctgtat
     2041 tgttataccc ccttccacag aaaaaacagt aataataatt aatataaaaa aacaaactat
     2101 tctcctcatt tttcatatgc tccaaattct gcaagatgtc caaaagaaga aaattctatc
     2161 ctgaatgctt ctgtttctgg aatatctatt tcatattcat ggacaactgc ttgacaaagt
     2221 gaaattccct tttgttctgg tgaaaattct acccacttat ttatttcatc atcccagaaa
     2281 agatgatata tctttggttc ttttaaattt atcaaatgcc aattattatc agaaaaatat
     2341 tttacattat atgaaatttt atttaagtat tgcggagaaa aaactaatct tattctcttt
     2401 atattgcttt tttttcttaa atttatttct acataaggtg aattatcatt ataagaaagc
     2461 caatcactta tataaccacc atcaattgca gcaaaagaac aacgatatgg gttgcatgat
     2521 gataccttta ttttccctcc ttcccatcct aatgctaaat tttcagaacc acttacccca
     2581 atccatgcat cttcttctct aaaattctcc aatgtttttc taaatggtaa atctggaact
     2641 ggttgactaa tataaatatg tgtctgataa ggggcaaatt catctgttat tttatttcca
     2701 tctcctttaa cctgtctatt ctctgaaaga acccaatatg tatttttatc aacaggtata
     2761 tctatttggt ctcttgtatt ccttgtatta actgcaaaaa tatattcaac gccatttaat
     2821 tctttcttca aataatgaac tgattttatt ttagaccaat taacctcata agaagataat
     2881 aatactggtt ctaaataatg tatttctgaa ctaaattctt ttaaagcaga ccacaaagca
     2941 ggaacatcta ataaattaga agaagaagag gaaaatgccc aatagataat cccttttgct
     3001 ccatgaatta gtgatagata tgtcattgct cttatttctt gaattgttgg ataacgggat
     3061 acctctggat ccccaccaaa ataaccactt ggaaaacatt gaagaactgc ccacactggc
     3121 tttttgtttc cacaggactt tactgcttca tctataaata tagatattct ttctaatggt
     3181 ctcataggaa aattcttatt tttctcaaaa cctaagtaag ggtccatcat tattatatct
     3241 gttaggtctt tatctaattt tatacctcct gtggtatcat gaactacact aattggccta
     3301 taagggtcca tatctctcat aagttttaag gcatccctgt atgctgacct atttaggtcc
     3361 ttgtgaattt caggctcatc tgcacaatac caaccaacta tacgatatgg attctgagaa
     3421 attaggtctt gaactttttg aatacctgct tgatttaatt gacttgaacg agcatgagta
     3481 aataatccaa tagttgaagc aaactcccat gcttgatttt cttctaacca ctctaatgtc
     3541 tcttttgatg ggagagaata actaaatata aaattatatc cacttttctt gtaattaaca
     3601 caagatgaaa cactataaaa tcctaatgga aaaactggtt tcccttttac tacaaaatta
     3661 ttgttttcat ctatccaaac ttctgttcct tctgttggag atagtttttt taaaggaatg
     3721 accatctccc ctttatctga ttttatttga ataaaatatt ctcctacttt taaatctggt
     3781 ataggaatag atatttcttt ttctttacct ttaaatgaaa aagatttttg aactatttct
     3841 ttattgtctt tgccttttat ttttattgtc ccttttagta aagaatttga agataaattc
     3901 tctattttaa taataataga agatattttt tgtgatggaa aaatactatt tctgtaatat
     3961 ggcttcaata aactgacaga aaaagaaagt ggcggaactt ctacctcatc ttgccaaaag
     4021 tatacaattt catctccttc ttttaattca cactttattt tatattctcc ttcttttata
     4081 agtgagattg gaaaatttaa ttctgttttc ccagaagaaa agaaaatttc tttttgttct
     4141 tgatgataaa aattgtcaac acctgaaagt aaaaaattta atatatatgt ttttccatct
     4201 ccttgattgc tgaaatctat tattaaattg ccatttataa ggccattttc tcttgataaa
     4261 aagagaaatt ttgcattttc tactttgata actgatgata tttgggcatt cactccttct
     4321 aaaaaagcaa acttttctgg attatgaaaa cccctttctt tccccattgg tggccataca
     4381 cttaattctc catttatttt attctcacga gaaaaactta ttcgccaatc agaagataaa
     4441 aaattatcaa aaagtccaaa aggtattgac atctctacta tatatttatc actaaacacc
     4501 ctcgattgca cttcccaact gcctccccat tcaggaatgt ctatactccc ctcttctatt
     4561 ctcttttcat cgtattttat acctaaggta ttggctacaa aatgataata tgggtctgca
     4621 tcagaaaaag gggaaacaaa taactctaca caatcatcat tccatacttg cttgtcattc
     4681 tcaataactt ttgcctctaa tttgtcaata tctttttctt tacaaataaa tgccacaaat
     4741 attctcttgc cattatgcca tattactgct tcactttcct gcgtcggtat ttctttacct
     4801 ccaagtaaat aaaaaggacc cgttgatact gcttttttcc atacatcttc ttctatatta
     4861 ccatctataa ttacattatc ttgaaaaaat ggaattctta tcctcggtat ttcttcactt
     4921 attacaggat aatttaaaaa aagataaaaa ataaacatat ttactacttt tttaaaatat
     4981 ttcttattca tttttatctc ctctttttct gtttctcttc cttttttaac aacccatccc
     5041 cttttgaaat ctcccctgaa aattccctct tactcccgtt gtacctattg aaatacatta
     5101 cggcaccaac tctacttctg ctactccccg ctacttctgt cggttttaca aaaggggaat
     5161 tttaactcct tccttttttc cttttcatct ctctttcatc taagtttttg acaatacctt
     5221 ctgtttacca gttaactgct
//
LOCUS       DAOOVW010000074         5143 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_9085_length_5143_cov_6.418239, whole genome shotgun sequence.
ACCESSION   DAOOVW010000074 DAOOVW010000000
VERSION     DAOOVW010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9085_length_5143_cov_6.418239"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(233..1441)
                     /locus_tag="PLW95_07770"
     CDS             complement(233..1441)
                     /locus_tag="PLW95_07770"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOV22551.1"
                     /translation="MAIVSLEKMKKFIDIPEAAEILLTRSEKEIAFNVNLKTGRDEII
                     FCDAFVVYHNVALGPAKGGIRISPDVSLDETRTLAQIMTYKNALAGIPFGGGKSGIKI
                     NGGNIDEFKRVAIIKEFVHMIKEELIFGYYVPAPDLGSGPEDMAVIYGETHIPESVTG
                     KPVRIGGLPGRNEATGRSVAMSTYLVSEKILKEKISNCSVAIQGFGNVGRNTAIFLSE
                     MGAKIVAISDIFGGTYNGEGINIKELLSYAPTKDKSVLDFPEGENIKNEELLNLNVDF
                     LIPAAAGEVINETNAEDIKAKVIIEGANDPVNEKADEILEKKGVIILPDILCNSGGVI
                     ASYIEWRNAKSGNQTNKKEVYQIIDRKISDSFEKILNIKNNLRSSYRIAATYISLNEL
                     IEAMRERRWL"
     gene            complement(1924..2820)
                     /locus_tag="PLW95_07775"
     CDS             complement(1924..2820)
                     /locus_tag="PLW95_07775"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HOV22552.1"
                     /translation="MGKNFLLFIFTFCFIPFTLFSKEIYTVSVISGKIKLDGKLDEDV
                     WKKVKAIDNFYILGAGNPTTYKTEVKIGISEDSLYIGVICYEEKMDKIKAKYSDGGIL
                     WEEDSIEIFIYPEGAETYYQFVINAIGSKWNSSSSIQLSDWQAKVYKGNDYWSIEVRI
                     PFEVLGRYPEGVWTGNICRNRCVSDKQEFCSWAKILIGGFHQPDIFNELIFEKNNEIA
                     SKQLRKKVGNKIKELEKYIVKGNKKTISINQKITVSDTYQILNILYKFEDKITKESEQ
                     LSEQLKRKINAIEYNKLKRAFF"
     gene            complement(2842..4590)
                     /locus_tag="PLW95_07780"
     CDS             complement(2842..4590)
                     /locus_tag="PLW95_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22553.1"
                     /translation="MKQMIFVCLFLLCFVLPIQAEEVKVFVLTYPVSNQRVLPNEPVF
                     VPTTSEILIIATPGEYEPGSFVLNSKENLQQVEIEVSDLKSKEGEIIKSSSVDIKIVK
                     CWYQDKGRGYIEKTPARGYSPIGYSGKKVLVPELLLNDDNLIKVDYEKGKNYIKIKTE
                     KEEKYIDITPGNDVEEIEKQLPDGYNKWSGMTLVKDSEKLLPFELKENENKQVWLTIK
                     IPEDQKAGIYEGNVGIKNKGKELVNIVMKINVLPFKLCSYYPISSIYCQGMSRDLKNF
                     KKENEDLYTHGILEPYFHDTYVDKNFEEIKDDFGQRLKILQEIGLYKRGFYFFCGRTR
                     HFIEPTSDNLNKLKENVKKIVDFGRSYGINDVYFYGIDEAREERLTAQRPVWEAIHEA
                     GGKVFVAGYKGDNFSLMGDIQDLLVCAGYPSREEAEKWHAKGHKIVCYGNPQGGIEAP
                     DTYRRNFGILLWQYNYDGAMTYCYHWEIQGFGPMWSDWCERKYKAHNMVYPTGDGVID
                     TLQWEGYREGIDDVRYLNVLIEEVKKAKDSQMVKKEAQKFLEELKKGDINRDIEDMDT
                     LRLKIIDYIMKLKGKQ"
BASE COUNT         1509 a         1043 c          593 g         1998 t
ORIGIN      
        1 agtaaaaact gacttaaaaa gaaatttaag attttttata agtggaaaca aatttatttc
       61 aaaataataa agtgcaaaat gtaaagtaca aagcgtaaaa ttaaaacgaa aaattgaaaa
      121 tgaagaacaa gaaaacaaaa aagttttgaa ctttaagtta tagttttaca ctttgctctt
      181 ttaattttaa atttatactc tgtacttctt agttaacaca gttgactgag tattatagcc
      241 atcttctttc tctcattgct tctataagtt catttaaaga aatataagta gcagcaattc
      301 tataagatga ccttaaatta tttttaatat tcaaaatttt ctcaaatgag tcagatattt
      361 ttctatcaat gatttgataa acttcctttt tatttgtttg attacctgac tttgcattcc
      421 tccactcaat ataagaagca attactcctc cactattaca taatatatct ggtaaaatta
      481 taacaccctt tttttcaaga atttcatctg ctttttcatt aacggggtca tttgctcctt
      541 caataattac ttttgctttt atatcttctg cgtttgtttc atttattact tctcctgctg
      601 ctgcaggaat aagaaaatca acatttaagt ttaataattc ttcatttttt atattttcac
      661 cctctggaaa atcaagtaca cttttatctt tagtcggtgc ataacttaaa agttctttaa
      721 tgtttatacc ttcaccatta tatgttccac caaatatatc actaatagca acaatttttg
      781 ctcccatttc agacaaaaat atagcagtat ttcttccaac atttccaaat ccctgaatag
      841 caactgagca attagaaatt ttttctttta aaatcttttc actaactaaa taggttgaca
      901 tagcgacact tctaccagtt gcttcatttc taccaggtaa tcctccaatt cttactggct
      961 ttccagtaac tgattcagga atatgtgttt ccccataaat aacagccata tcctcagggc
     1021 cactgccaag gtcaggtgca ggtacataat atccaaaaat taattcttct tttatcatat
     1081 gaacaaattc tttaattata gcaactctct tgaattcatc aatatttcct ccatttatct
     1141 taattcctga ttttcctcca ccaaatggga ttcctgcaag tgcatttttg taagtcataa
     1201 tttgagcgag agttcttgtt tcatctaaac ttacatcagg agaaattctt ataccacctt
     1261 ttgcagggcc taaggcaaca ttatgataaa caacaaatgc atcacaaaaa ataatttcgt
     1321 ctcttcctgt tttcaaattt acattaaaag caatttcttt ttctgacctt gttaataaaa
     1381 tttctgctgc ttcaggtata tcaataaact ttttcatctt ttcaagagaa acaatagcca
     1441 tttgttcttg tatgatgaag ttagagtttt tagaggagtt atttagagta attatactcc
     1501 ttgtgaactc tattacttca catacaataa tcacctcctt taaaggtttt aattttaagg
     1561 aaggggaaag aaagcggata tttttcctgc tatttataaa agcacgacga agtttgcttc
     1621 tttctccttc ttgttttttg tgttttaata tatcagtaaa cagggaaaag agagtagcac
     1681 gttttcctta ccctttaaaa gggaataaaa tgagagcatt ctacggaaaa ctttatggag
     1741 tagttactga aaataaagga aattacggct ttgtccattt gaataaacca aatggctcta
     1801 accgaatatc aaaagcaagg ttacagacca taaagtttcc ttttttctct ttatctgaca
     1861 caaaagaaat caaacctctt ttcctccatc cctttctttt ttaataagta ttctcttacc
     1921 attttaaaaa aatgcccgct ttaacttatt atattctatt gcatttattt ttctttttag
     1981 ttgttctgat agttgttcgg attctttcgt tattttatct tcaaatttat acaggatgtt
     2041 taatatctga taagtatctg agacagtaat tttttgattt atagatattg tttttttatt
     2101 tccttttact atatattttt ctaattcttt tattttattt cctacttttt ttcttaattg
     2161 ttttgatgca atttcattgt ttttttcaaa tatcaactca ttaaaaatat ctggttgatg
     2221 aaatcctcca attaatatct ttgcccatga acaaaattct tgtttatcag aaacacatct
     2281 gtttctacat atattccctg tccatacacc ttctggatat ctacctaaca cttcaaatgg
     2341 tatccttact tctattgacc aataatcgtt ccctttatat acctttgctt gccaatcgga
     2401 aagttgaata gaactacttg aattccattt actaccaatt gcatttataa cgaactgata
     2461 atatgtctct gcaccttctg gatatataaa aatttctata ctatcctctt cccataaaat
     2521 cccaccatct gaatacttcg cttttatctt gtccattttt tcttcataac atatcacccc
     2581 tatatataaa ctatcctctg atataccaat tttcacttct gttttatagg tagttgggtt
     2641 ccctgctcca agtatgtaga aattgtcaat cgcttttact tttttccaga catcctcatc
     2701 taatttccca tctaatttta ttttcccaga aataacagaa acagtatata tttctttgct
     2761 aaataaagta aatggaataa aacagaatgt aaagataaat aaaagaaaat ttttccccat
     2821 ttgaaattcc tcctttcttc tctattgctt cccctttaat ttcattatgt aatcaataat
     2881 ctttagtcgt aaggtatcca tatcttctat atctctgttt atatcccctt tctttaattc
     2941 ttctaaaaat ttctgtgctt cttttttcac catctgacta tcctttgcct tttttacctc
     3001 ttcaattaat acattcaaat acctaacatc atctatccct tctctatatc cctcccattg
     3061 taatgtgtct attactccat cacctgttgg atataccata ttatgtgctt tatattttct
     3121 ctcacaccag tcagaccaca tcggtccaaa tccttgtatt tcccaatgat aacagtatgt
     3181 catagctcca tcatagttat attgccagag aagtattcca aaatttcgtc tgtatgtatc
     3241 tggtgcttct atccctcctt gtggatttcc ataacaaaca attttgtgcc cttttgcatg
     3301 ccatttttct gcttcttctc ttgatggata accagcacaa acaagcaaat cctgtatatc
     3361 ccccattaaa gaaaaattat cacctttata tccagcaaca aatactttac ctcctgcctc
     3421 gtgtattgct tcccagacag gcctttgagc agttaatctt tcctcccttg cttcatcaat
     3481 accataaaaa tatacatcat ttatcccgta agatctccca aaatctacaa tcttttttac
     3541 gttttctttg agtttgttca aattgtcaga agtcggttct ataaaatgtc ttgttctccc
     3601 acaaaaaaag taaaaacctc ttttatagag acctatttcc tgtaaaattt tcagtcgctg
     3661 cccgaaatca tccttaattt cttcaaaatt tttgtcgaca taagtatcat gaaaatatgg
     3721 ttctaaaatc ccatgggtat ataaatcttc attttctttt ttgaaatttt ttaaatcccg
     3781 gctcattccc tgacaataaa tagatgaaat gggatagtaa ctacataact tgaatggtaa
     3841 aacatttatc ttcattacaa tattcaccaa ttctttccct ttattcttta ttcctacatt
     3901 accttcatat attcctgctt tttggtcttc tggaattttt attgtcaacc agacctgttt
     3961 attctcattt tcttttaatt caaaaggaag gagtttttca ctatccttta caagtgtcat
     4021 cccactccac ttgttatagc catctggtaa ttgtttttcg atttcttcta catcatttcc
     4081 tggtgttata tcaatatatt tctcttcttt ttctgtcttt atctttatat aatttttccc
     4141 cttttcatag tcaactttta ttaaattatc atcattaagc aataattctg gaactaatac
     4201 cttcttgcca ctatatccaa tgggactata tcctcttgcg ggtgttttct ctatatatcc
     4261 tctcccttta tcttgatacc aacactttac tatttttata tctactgatg atgattttat
     4321 tatttcccct tctttactct ttaaatcact tacttctatt tctacctgtt gaagattttc
     4381 tttgctgttt aatacaaaac tccctggttc atattctccc ggtgttgcta taattaaaat
     4441 ctcagatgtg gttggaacaa ataccggctc atttggtaac accctttgat ttgatacagg
     4501 atacgttaat acaaatacct tcacttcttc tgcttgaata ggcaaaacaa aacataataa
     4561 aaacaggcag acaaatatca tttgcttcat aattccctcc ttttcttatt attggtactg
     4621 ccaatctttt ataatgcctt tctttgtctt ttaatgcttt tatatgtctt tatatccctc
     4681 cccctctttt catctatttt tttgacacta cctccccgtt gtgctatctg ggagacgatg
     4741 acgcaccaac tctgccctgc taccatttgc ttccttagtc gggagcagtt gtgctatctg
     4801 aaatccccct ttatccccct ttgttaaagg gggaaataaa gcaccaactc tgctctgcta
     4861 agccctgctc cttcagtcgg tttgttaaag ggagaactta cttaccttat tgtttcaaga
     4921 acccggtttt tacagaaaac caggttcttc tatcttaaca tttttcaaag ggggaattta
     4981 tgggtaatct ctatccttat ggcccgaggg gtggatttgt tctttacttc tttctactct
     5041 ctactcgcta ctttcagtca gtgtgattga gtaagtatat acgaagaact tctaccaaat
     5101 aaactcgcta agaaaataaa aactctctta attctgaaaa ccc
//
LOCUS       DAOOVW010000075         4989 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_9429_length_4989_cov_8.459465, whole genome shotgun sequence.
ACCESSION   DAOOVW010000075 DAOOVW010000000
VERSION     DAOOVW010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4989
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9429_length_4989_cov_8.459465"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(299..436)
                     /locus_tag="PLW95_07785"
     CDS             complement(299..436)
                     /locus_tag="PLW95_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22554.1"
                     /translation="MKKKDEKEKGCFSESKTEKKLFIENFGNRKKHRKSEKRGLKVNR
                     A"
     gene            complement(567..1280)
                     /locus_tag="PLW95_07790"
     CDS             complement(567..1280)
                     /locus_tag="PLW95_07790"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOV22555.1"
                     /translation="MNILAVGAHPDDLELLCGGTLAKYSKKGHKIFMAHLCNGNLGGK
                     DITPDQLAEIRDKEAKKSGSLINAEVLGPIASDLDLYPTKEMRKEVVDIIRHARPDII
                     ITHSPDDYMPDHTTTSKLVFDAAFTATLPNYKTKETFWEKITPIFYMDTSVGLKFQPD
                     YYVDITEFFEIKKKMFLCHESQYKWLKGHHLSDPLNIIETMGKFRGLQCGVLYAEGFV
                     IEKVWGRVNPTELISGITL"
     gene            complement(1314..2264)
                     /locus_tag="PLW95_07795"
     CDS             complement(1314..2264)
                     /locus_tag="PLW95_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HOV22556.1"
                     /translation="MNLIDTIKGSLLENFFPKGWNLEKIDRCCSFPPEKIFEKQNWWN
                     KNFQPIACDTLEEFNLKMGHEIALEISQTKKEGKKLILILPVGPMGMYKWAVYFLKEW
                     KVDCEHVYGFNMDEWSDKNGNTLPPDNPGAFQNAMEGAFYGPLGKLTVPVKQRFFATK
                     ELLPKYPEKISKLRGEGARMVLIFGIGRVFHIAFWEPHFAEEFKSEKEWISQMYRTGA
                     KLHPLTIEQNAITSFKSRTTLVPCFANTIGPGIFLKGDKIIGGADGILGRGMMWQGMS
                     LWVTLRYGPDIWVPSSFIPTLPGKLFFLKELAGPLIPECN"
     gene            2349..3611
                     /locus_tag="PLW95_07800"
     CDS             2349..3611
                     /locus_tag="PLW95_07800"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013873453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOV22557.1"
                     /translation="MEKLAIEGGSPVNTRPFPMWPSFSQKSIEMVAEPLKNGKVNYWT
                     GDIGNKFEKEWAKWNGAKYCITTTNGTSALHTAVSALNIGPGDEVICPSYSFIASSFC
                     ILQAGAIPVFADVEKNSHTLDPNDIEDKITDRTKGIIVVHLYGIVANMYPILEVARKH
                     NLFVIEDCAQCFGGIYKGKKTGTIGDVGCFSFCQSKHFTTGGEGGAVITNNEDLYWEC
                     MSFRDHGYDVKERLRLLEMEKKLMYIHKRVGFNYRMTEIQSLIGLCELERFDTWNLAN
                     RKRNGKFLISALKDHPLVLYPPLDTEERQNAFWWAPFVIDTEKLKVPLKQFIAAIEAE
                     GVPAYGVQWPEMYKERAYIEKNGFGRLKYPFDDPNARKIDYSKVECKTAKWLSDRTMS
                     FFTHPVYEMEHMEKCVEAFNKVANVYMK"
     gene            4065..>4989
                     /locus_tag="PLW95_07805"
     CDS             4065..>4989
                     /locus_tag="PLW95_07805"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOV22558.1"
                     /translation="MRKVNMGVIGAGGIAKRRSIPGMLDAKNIKVVAVMDVIGAKKIA
                     DEFKIDKYYEKEEDMVKDEEVECIYIASPAFLHLKHIKLSAENGKHILCEKPLCINLK
                     DGKKAVEICKKNNVFIQEGYMMKFHGAHKKIKQLIEEGKLGRIVYMRAQLSCWYPPIE
                     GAWRQDPKKGGGGSLIDMATHLYDLLEFLIDKKVKRVSAITNNLVHFYKSEDSSTTLL
                     EFVDGTHATVDSFFCIPDEASKTRLEIYGSKGAILTEGTIGQSIGGKMEGIFGVGEGG
                     YDAIQSKDKIPQFEEIPFEKINPYTAEFEYFA"
BASE COUNT         1619 a          808 c          862 g         1700 t
ORIGIN      
        1 gaatatctat aaacattttt tctccttttg tttataaagg ttatgccaaa attggctata
       61 gcttttataa tgcctttctt tgttttttaa tgcttttata tgtctttata tccctccccc
      121 tttttttggt aaaataatat gaaaaaagga ggttttttat gtctcaaaca agtaatgtta
      181 atcgttttgt tctaggggcc cgccggattc tgcgtacata ttgcctctaa attgccctaa
      241 atctttttta aataacttta tacctatttc ttgatatagt ccctgcctta ttgctcattt
      301 atgccctatt tacttttagt cctctttttt cactctttct gtgctttttt ctattcccaa
      361 aattttcaat gaacaatttt ttctctgttt tgctttcaga aaaacaccct ttttcctttt
      421 catctttttt tttcatctat tttttgacac taccttatat agttcccatc taaatattcc
      481 catgcaccat tttgatattc taaaaagcag atactttatc ttctacttca atattatgca
      541 gttttgttct tgtttttgct tcaattttat aaggtaatcc cgctaataag ttcagttgga
      601 tttactcttc cccagacctt ctcaattaca aatccctctg catacaaaac accgcactgt
      661 aatcccctga actttcccat tgtttcaatt atatttaaag ggtcagaaag atgatgtcct
      721 tttaaccatt tatattgact ctcatgacag agaaacattt tcttttttat ctcaaaaaat
      781 tcagttatat caacataata gtcaggctga aatttaagtc caacggaagt atccatataa
      841 aaaattggag ttattttttc ccagaatgtt tcttttgttt tataatttgg tagtgttgca
      901 gtaaaagcag catcaaaaac aagttttgat gtagttgtat ggtctggcat ataatcatca
      961 ggactatgag tgataattat atctggtctt gcatgtctta taatatcaac aacttctttt
     1021 ctcatttctt ttgttggata aaggtcaagg tcagatgcaa ttggtccaag aacttctgca
     1081 tttatcaaac tacctgattt ttttgcttct ttatccctta tttccgcaag ttggtcaggt
     1141 gttatatcct ttcctcctaa atttccatta catagatgag ccataaatat tttatgccct
     1201 ttttttgaat attttgctaa tgttccacca cataaaagtt caaggtcatc tggatgtgct
     1261 ccaaccgcta atatattcat ttttttctcc tttgaaggaa taccctcaca ttattaatta
     1321 cattcaggaa ttagtggtcc tgcaagttct ttcaagaaaa ataattttcc tggtaaagtt
     1381 ggtatgaaac tgcttggaac ccatatatca ggtccatatc ttaaagtaac ccataaactc
     1441 attccctgcc acatcattcc tcttcctaat atcccatctg ctcctcctat aattttatca
     1501 cctttcaaaa atattccagg tcctattgta tttgcaaaac agggaacaag agttgttcta
     1561 cttttaaaac ttgttattgc attttgttca attgtaagag gatgtaattt cgctcctgtg
     1621 cgatacatct gagaaatcca ttccttttct gacttaaatt cttctgcaaa atgtggttcc
     1681 cagaaagcaa tatgaaaaac cctgccaatc ccaaaaatta aaaccatcct tgctccttct
     1741 cctctcaatt ttgaaatttt ttcaggatat ttcggtaata attcctttgt tgcaaaaaat
     1801 ctttgtttta caggaacagt caattttcca agtggcccat aaaatgcacc ttccatagcg
     1861 ttttgaaaag cgcctggatt atcaggtgga agagtattgc catttttatc actccattcg
     1921 tccatattaa aaccataaac atgttcacaa tctactttcc actctttaag aaaatacaca
     1981 gcccatttat acattcccat tggtccaaca ggcaaaatta aaattaactt tttcccttct
     2041 ttttttgttt gagatatttc cagagcaatt tcatgtccca tcttcagatt aaattcttca
     2101 agtgtatcac aggcgattgg ttgaaaattt ttattccacc aattttgctt ttcaaaaatt
     2161 ttttcaggtg gaaaagaaca acatctatca attttttcta aattccatcc ttttgggaaa
     2221 aaattctcta aaagcgagcc cttaattgta tctattaaat tcataaatac ctccctgttg
     2281 acataggttt ttttctatta taacatactt ttaaaggtaa aaaaatttaa taaaaaaagg
     2341 aggaaagaat ggaaaaatta gcaattgaag gtggaagtcc tgtaaataca agaccatttc
     2401 ctatgtggcc ctctttttct caaaagtcaa tagaaatggt agcagaacca ttgaaaaatg
     2461 ggaaagtgaa ttattggaca ggagatattg gcaataaatt tgaaaaagaa tgggcaaaat
     2521 ggaatggtgc taaatattgt attaccacaa caaatgggac aagtgcttta cacactgctg
     2581 tttctgctct taatattggt ccaggtgatg aagtaatatg tccttcttat tcttttattg
     2641 cttcttcctt ttgtatttta caggcaggag caatacctgt ttttgctgat gttgagaaaa
     2701 actcacatac tcttgaccca aatgatatag aagacaaaat tactgataga acaaaaggta
     2761 taattgttgt tcatctttat ggaattgttg ctaatatgta cccaattctt gaagttgcaa
     2821 gaaaacataa tctttttgtt attgaagatt gtgctcagtg ttttggagga atttataagg
     2881 gaaagaaaac gggaacaatt ggagatgttg gttgttttag tttctgccag agcaaacact
     2941 ttacaacagg tggagaggga ggagcagtta ttacaaataa tgaggacctt tactgggaat
     3001 gtatgtcatt tagggaccat ggatatgatg taaaagagag attacgactt cttgaaatgg
     3061 aaaaaaaatt aatgtacata cacaaaagag ttggttttaa ttatagaatg acagaaatac
     3121 aatcattgat aggtctttgt gaacttgaga gatttgatac atggaattta gcaaatagaa
     3181 aaaggaacgg gaaatttcta atttctgcct taaaagacca tcctcttgtt ttatatccac
     3241 cacttgatac agaagaaaga cagaatgctt tttggtgggc tccatttgtt attgatacag
     3301 agaaattaaa agtcccttta aaacaattta tcgcagcaat tgaggcagaa ggtgttcctg
     3361 cttatggagt tcaatggccc gaaatgtata aagaaagagc atatatagaa aaaaatggtt
     3421 ttgggcggtt aaaatatcca tttgatgacc ctaatgcaag aaaaattgat tactcaaaag
     3481 ttgaatgtaa aacagcaaaa tggttatcag ataggacaat gtctttcttt acacatcctg
     3541 tatatgaaat ggaacatatg gagaaatgtg ttgaagcatt taataaagtt gctaatgtct
     3601 atatgaaata gtagtatgct acaaaataag ggtttcgttt ttgataattt tattcttttt
     3661 tgttttttgt agaaattatt ctcccttttg taaaccgact aaggaagcaa aggggtagca
     3721 aagcagagtt ggtgcgtcat agtttcttaa atagcacaac aggggcaggg ggagatttaa
     3781 gacaaatcca ttcctacctt cctttataaa aggaaggagt tttttctccc tttataaagg
     3841 gagattaaga gggatttcat ccttctcccc ttagcctgtc cgacaagatt tgtggcagag
     3901 ggagaaggtg gggctcgccc gtagggctac gcccgaggga tgagggggtt ttcagatagc
     3961 acaacggggg ttagagagat tttggaaatt aaagttctct ctacttgtta tttaaatata
     4021 aactggttag tgaaaaattt attattccaa taagaggagg gattatgaga aaagttaata
     4081 tgggagtaat aggggcaggt ggtatagcaa aaagaaggtc aatacctgga atgctggatg
     4141 ctaaaaacat aaaagttgtt gctgttatgg atgttattgg agcaaaaaaa atagcagatg
     4201 aatttaaaat tgataaatac tatgaaaaag aagaggacat ggtaaaagat gaagaagttg
     4261 aatgtattta tattgcatct cctgcttttt tacacttaaa acatataaaa ctttcagcag
     4321 aaaatggaaa acatatatta tgtgaaaagc cgttatgtat aaatttaaaa gatgggaaaa
     4381 aagcagtaga gatttgtaag aaaaataatg tttttattca agaaggatat atgatgaaat
     4441 ttcacggagc acataagaaa attaagcagt taatagaaga aggtaaatta ggaagaattg
     4501 tatatatgag ggcacaactt tcctgctggt atccaccaat agaaggggca tggcgacagg
     4561 accctaaaaa aggcggaggc ggttctttaa ttgatatggc aactcatctt tatgaccttc
     4621 ttgaatttct aattgataaa aaagtaaaaa gagtatctgc aattacaaat aaccttgtcc
     4681 acttttataa atcagaggac tcatcaacaa ccttacttga atttgttgat ggaactcatg
     4741 caacagttga tagttttttc tgtattcctg atgaggcgag taaaacacga cttgaaatat
     4801 atggttcaaa aggagcaatt ttgacagaag ggacaatagg tcagagtatt ggtggaaaaa
     4861 tggaaggaat ttttggtgtc ggggaaggcg gatatgatgc aatacagagt aaagataaga
     4921 ttccacaatt tgaagaaatc ccctttgaaa aaatcaatcc ctatactgct gaatttgaat
     4981 attttgctg
//
LOCUS       DAOOVW010000076         4967 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_9474_length_4967_cov_8.019544, whole genome shotgun sequence.
ACCESSION   DAOOVW010000076 DAOOVW010000000
VERSION     DAOOVW010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4967
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9474_length_4967_cov_8.019544"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..1271
                     /locus_tag="PLW95_07810"
     CDS             93..1271
                     /locus_tag="PLW95_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOV22559.1"
                     /translation="MEKKKIESERIKKLPPYMFQEINARKLKLRREGKDIIDFGMGSP
                     DKPPAKPVIEKLKEVVDNPKVHGYSASKGITHLRKEICTWYGKRFGVKLDPEEEAIVC
                     IGSKEGISHLSLSIFDKGDVVLIPNPTYPSHLYSAIIAGASVYDIPLTKENNFIPDFS
                     HPIYDRFPYPKCMILSYPNNPTTQVVEIDFFKEVVKFAKKNNLIVIHDNAYSEICFDG
                     YKAPSFLQTPGAKNIGIEFYSLSKTYNMAGWRVGFAVGNKYIIQALTKLKSYYDYGIF
                     TPIQVAAITALRLEQKYIDEVVETYRKRRDVLVNGLMRIGWKVEKPKATMYIWAEIPE
                     KYKKMGSMKFAMYLLEKGNVSVSPGVGFGKYGEGYVRMALVENEQRIKQAIKSLRKII
                     "
     gene            complement(1303..2910)
                     /locus_tag="PLW95_07815"
     CDS             complement(1303..2910)
                     /locus_tag="PLW95_07815"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012621017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOV22560.1"
                     /translation="MKKNKKFIFITGGVVSSLGKGIASASIGSLLESHGYKVTFLKFD
                     PYINVDPGTMSPFQHGEVYVTEDGAETDLDLGHYERFTDAKMTRKNNATSGQIYYTVI
                     SKERKGEYLGKTVQVIPHITNEIKKRVFDLIDKVDFIITEVGGTVGDIESLPFLEAIR
                     QIQITLPEEDTLHIHLTLIPYIRAAEELKTKPTQHSVGRLREIGIQPKILLCRTEKPL
                     GDEIREKIALFCNVKKEAVIEAIDTDLIYEIPLIFAHQKLDRIILKYLGMRGNKNNLS
                     KWENIVKALKNTKKEVVIGVIGKYIKLQDSYKSIYEALIHGGIGNETKVSFKKIDSED
                     IEKNSAEKYLIDVDGAIMPGGFGLRGVEGMIKAIKYIRENKIPFLGICLGMHCSIIEF
                     ARNVCTLQDANSSEFNPKTKNPVIDLMEQQKKISKMGGTMRLGAYRCVLKKGTISYSS
                     YGKNKIYERHRHRYEFNNTYKDIMEEKGMVFAGINPDNGLIEIIEIKEHPFFVATQFH
                     PEFKSKPFAPHPLFSAFIKASILNIKR"
     gene            complement(2934..3683)
                     /gene="kdsB"
                     /locus_tag="PLW95_07820"
     CDS             complement(2934..3683)
                     /gene="kdsB"
                     /locus_tag="PLW95_07820"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF009905.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOV22561.1"
                     /translation="MRKRIICVIPARYGSERLPGKVLYKIGEKTMLQRVYERAKIVGI
                     FDKIYIATESRKVQEECEKFKYEVIWTSENCRTGSDRVAEAIGKIKEKIDIVVNIQAD
                     EPFLPIEAVKKPLEIMLGNPSINISTSVTKIREKEDLYNPNVVKVVLDKENNALYFSR
                     SLIPYPRIYFNPDKLYSSKKVVFYKHIGVYIFRKVFLEKFAKMETTFLESIEKLEQLR
                     IIENGYKIKTAIVKKDSPCVDTIGDIEKLKM"
     gene            complement(3680..4720)
                     /gene="rfaE1"
                     /locus_tag="PLW95_07825"
     CDS             complement(3680..4720)
                     /gene="rfaE1"
                     /locus_tag="PLW95_07825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HOV22562.1"
                     /translation="MEKEKLSRFLSQKKLKEVLEKSQRKNILVVGDVILDNYIYGDVS
                     RISPEAPVPVVEVKEESFTAGGAGNVALNIKSLGGNVFLIGGIGKDTNGEILTHIIKE
                     KGIETFFLKRSLSSTITKTRIIGRDQQIVRIDREKRKEISDKEVKQIIGKIDEIVNNK
                     IDGIIVSDYGKGIITKKLINYLIDIKKKRGIILCIDPKIQHFTYYKNVTCLTPNKKEA
                     AEGMKENNPESKNEIIKLGKKIKKKLKSEILIITLGKQGMILFDRKNNIFHIPSVAKE
                     VYDVTGAGDTVIASFTLTFSQTKDEVISSIIANLAGGIVVGKLGTAVVTSEELEKFFE
                     KNLNSEYIKIEK"
     gene            complement(4721..>4967)
                     /locus_tag="PLW95_07830"
     CDS             complement(4721..>4967)
                     /locus_tag="PLW95_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22563.1"
                     /translation="WWPLHPIGFLIGPVWLMDNLWFSIFIIWLIKKLILKYGGANIYE
                     KLKYFFLGLPLGLYTCAGIWFIIDFFTGKHGNQIFWI"
BASE COUNT         1602 a          807 c          658 g         1900 t
ORIGIN      
        1 tcttttttgt ctcaacgata ccctttcgtg tagatttatt atgtctcaat tcgaagaatt
       61 gacagggaaa aatataaaga taaaataaag gtatggaaaa gaaaaaaata gagtcagaaa
      121 gaataaaaaa attacctcct tatatgtttc aggagataaa tgcaagaaaa ttaaaattaa
      181 gaagagaggg aaaagatatt attgattttg gtatgggaag tcctgataaa ccaccagcaa
      241 aaccggtaat tgaaaagtta aaagaagttg tagataatcc aaaagtacat ggatatagtg
      301 catcaaaagg aataacgcat cttagaaaag aaatatgtac ttggtatgga aaaagatttg
      361 gagtaaaact tgaccctgaa gaagaggcaa ttgtttgtat aggttctaaa gaaggcataa
      421 gtcatctgtc cttgtcaatt tttgataaag gagatgtagt tttaattcca aatccaactt
      481 atccaagcca tttatatagt gccattattg caggggcctc agtttatgat atcccattga
      541 caaaagaaaa caattttatt cctgattttt ctcatccaat atatgaccgt tttccctatc
      601 ctaaatgtat gattttaagt tatccaaata atccaacaac acaagtggta gaaattgatt
      661 ttttcaaaga agtagttaaa tttgcaaaga aaaataattt gattgtaata catgacaatg
      721 catattcaga aatatgtttt gatggatata aagcaccaag ttttttacaa acacccggag
      781 caaaaaatat tggaatagag ttttattctc tttcaaaaac atacaatatg gctggatggc
      841 gtgttggatt tgcagttggt aataaatata ttatacaggc acttacaaaa ctaaaaagtt
      901 attatgatta tggaattttt actcctatcc aggtcgctgc aataactgct ttgcgacttg
      961 aacaaaaata tatagatgag gtagttgaga catatagaaa aaggagagat gttcttgtaa
     1021 atggacttat gagaattggg tggaaagtag aaaaaccaaa agcaacaatg tatatatggg
     1081 ctgaaattcc cgaaaaatat aaaaaaatgg gttcaatgaa atttgcaatg tatcttcttg
     1141 aaaaaggcaa tgtttctgta tctcctggag ttggttttgg aaaatatgga gagggttatg
     1201 ttagaatggc tttggttgaa aatgaacaaa gaattaaaca agcaataaaa tcacttagaa
     1261 aaataattta gttatttttt cccgaaaaat ataagaatgt ttttatcttt ttatattcaa
     1321 aatagacgct tttataaaag cagaaaatag gggatgtggt gcaaatggtt ttgatttaaa
     1381 ttctgggtgg aattgagttg caacaaaaaa gggatgctcc ttaatttcaa taatttcaat
     1441 aagtccgtta tcaggattga ttcctgcaaa aaccattcct ttttcttcca taatatcttt
     1501 gtatgtgtta ttaaattcat atctatgtct gtgtctttca taaattttat ttttcccata
     1561 tgaggaataa gaaattgttc ctttttttaa aacacatcta taagcaccta atctcattgt
     1621 tccacccatt ttgctaattt ttttctgttg ttccataagg tctattactg gatttttggt
     1681 ttttggatta aattcactgc tattggcatc ttgaagggta caaacatttc tggcaaattc
     1741 aattattgaa caatgcatgc ctaaacatat acctaaaaat gggattttat tttctcttat
     1801 atattttatt gcttttatca taccctctac gcctcttaat ccaaaaccac ctggcattat
     1861 tgctccatca acatctatta aatatttttc tgcactattt ttttctatat cttctgaatc
     1921 aattttttta aaacttactt ttgtttcatt accaattcca ccatgaatta acgcttcata
     1981 aattgattta taagagtcct gcaattttat atactttcct ataactccaa ttacaacttc
     2041 tttctttgtg ttttttaatg ccttaactat attttcccat ttggaaaggt tatttttatt
     2101 acctctcatt ccaagatatt ttaaaattat tctatctaat ttctgatgtg caaatataag
     2161 agggatttca tatataaggt ctgtatcaat tgcttctatt actgcttctt ttttgacatt
     2221 acaaaaaaga gcaatttttt ctcttatttc atctcctaat ggtttttctg ttctgcataa
     2281 aagaattttt ggttgaatac ctatttctct aagtcgtcca acactatgtt gagttggttt
     2341 tgtttttaat tcttctgctg ctcttatata tggaataaga gtaagatgta tgtgtaatgt
     2401 atcttcttct ggcaatgtaa tttgaatttg ccttattgcc tcaagaaatg gcaaactttc
     2461 aatatctcca acagttcctc caacttccgt aattataaaa tcaactttat caataaggtc
     2521 aaaaaccctt tttttaattt catttgttat atgggggatg acttgaactg ttttgcctaa
     2581 atattctcct tttctttctt ttgaaataac agtatagtaa atctgtccac ttgttgcatt
     2641 gttttttctt gtcatttttg catctgtgaa tctttcatag tgtcccaggt caaggtctgt
     2701 ctcagcacca tcttctgtaa cataaacttc tccatgttga aatgggctca tagttccagg
     2761 gtcaacattt atgtatgggt caaactttaa aaatgttact ttatacccgt gtgattcaag
     2821 taaagagcca attgaagcag atgcaatccc tttccctaag gaagaaacaa ctccaccagt
     2881 tatgaatata aattttttat ttttcttcat atttataaat attcccttta attttacatt
     2941 ttaagtttct ctatatcacc aattgtatca acacacggtg agtccttttt aactattgct
     3001 gtttttattt tatacccatt ttcaataatt ctcaattgtt ctaatttttc aatactttca
     3061 agaaaggttg tttccatctt tgcaaatttt tcaagaaata cctttctgaa aatataaact
     3121 ccaatatgct tataaaaaac aacttttttt gaagaatata atttatccgg gttaaaataa
     3181 attcttggat atggaattaa agaacgagaa aaatataatg cattattttc tttatccagt
     3241 acaactttta caacattagg attataaagg tcttcttttt cccttatttt tgttacagat
     3301 gtagaaatgt taatagatgg gtttcctaac ataatttcta atggtttttt aactgcctca
     3361 ataggcaaaa atggttcatc tgcctgaata tttacaacta tatcaatttt ttccttaatt
     3421 tttccaattg cttctgcaac tctatcgctt cctgttctac aattttctga tgtccaaatg
     3481 acttcatatt taaatttttc acattcttcc tggactttcc tactttctgt tgcaatatat
     3541 attttgtcaa aaataccaac tattttcgcc ctttcataaa ccctctgtaa cattgttttt
     3601 tcaccaattt tataaaggac cttaccaggc aatctttctg aaccatatct tgcaggtatt
     3661 acacaaatta ttctttttct catttttcta ttttaatgta ttcggaattt aaattttttt
     3721 caaaaaattt ttcaagttct tcactggtta caactgctgt tcctaattta ccaacaacaa
     3781 taccacctgc caaatttgca attattgaag aaataacttc atcttttgtc tggctaaaag
     3841 ttagggtaaa agaggcaata actgtatccc ctgctcctgt tacatcatat acttcttttg
     3901 cgacagatgg aatatgaaag atattatttt ttctatcaaa taaaatcatt ccctgttttc
     3961 caagagtaat aataagaatt tcacttttaa gtttcttttt tattttcttt cctaatttta
     4021 ttatttcgtt tttactttca gggttgtttt ctttcattcc ttctgctgct tcttttttgt
     4081 ttggagttaa acaggtaaca tttttataat aagtaaagtg ctgaattttt gggtcaatac
     4141 atagaataat tcctcttttc tttttaatat caataaggta atttatcaat ttttttgtaa
     4201 taattccttt tccataatca gaaacaatta ttccatcaat tttattattt acgatttcat
     4261 caatttttcc aataatttgt tttacttctt tatcagatat ttcttttctt ttttctctat
     4321 ctattctcac aatttgctgg tctctgccaa ttattcttgt tttggtaatt gtagaagaaa
     4381 gggacctttt aagaaagaat gtttcaattc ccttttcttt tattatatga gttaaaattt
     4441 caccatttgt atctttacca attccgccta tcaaaaacac atttccacca agtgatttta
     4501 tatttagagc aacatttcct gctcccccag cagtgaaact ttcttctttt acttcaacca
     4561 caggaacagg tgcttctgga gaaattcttg aaacatcacc ataaatataa ttatcaagaa
     4621 taacatctcc aacaacaagt atatttttcc tctgtgattt ttcaagtact tcttttaact
     4681 ttttctgact taaaaacctt gataattttt ctttttccat ttatatccag aaaatttgat
     4741 tcccgtgttt tcctgtaaag aaatcaatta taaaccatat accagcgcat gtataaagac
     4801 ctaatggcaa ccctaagaag aaatatttca atttttcata aatatttgcc ccaccatatt
     4861 ttaaaattag tttttttata agccagataa taaaaattga aaaccataaa ttatccataa
     4921 gccatacagg ccctattaaa aaaccaattg gatgtaatgg ccaccat
//
LOCUS       DAOOVW010000077         4837 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_9796_length_4837_cov_9.460686, whole genome shotgun sequence.
ACCESSION   DAOOVW010000077 DAOOVW010000000
VERSION     DAOOVW010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4837
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9796_length_4837_cov_9.460686"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..2665
                     /gene="mutS"
                     /locus_tag="PLW95_07835"
     CDS             77..2665
                     /gene="mutS"
                     /locus_tag="PLW95_07835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOV22564.1"
                     /translation="MWYNFLMENLTPMLEQYNSIKKEYPDCIVFFRLGDFYEMFYDDA
                     KIASQILDLVLTSRSAGKMGKVPMCGIPFHSADSYISKLVKAGYKVAICEQVEDPSKA
                     KGIVKREVIRVITSGTFIDENSTEGRYLVSIYPDKKTIGISFLSSEGETIFTNQFDDI
                     TKVVDIIFKYPIYEIIFPEEKEEFIKNIFEQYPFLKLRKIVLSSCNDYTFNYEISKET
                     IFSHFKVESLRGFGIEEKELCVRTSGAIIEYLKFVNKKDFSHLNKISIYSDDDFLYIS
                     PSAFYGLEINKLVGILNIAVTPSGKRLLKDWIYHPLKKVEEVKERHKAVRFLKEKQEI
                     REKARNLLKGIGDVEKAVSKISCGYGNVKDIFIVANLIFKIPDIKKVISEIDIKNNYF
                     SVEDSDDLRNFLDMAINFDISPINYEGKLIKEGYNISLDELREISYKAKEYLRNLQKK
                     EIEKTGINSLKIGFNKVFGYYIEITKANLHLVPSDYIRKQTLVNAERFITPELKEFEE
                     KILTADTKIKEIEDKIIEEVKNKIIENAPTIYSITKKISELDVLICFASVSEENNYVM
                     PEIDEGFEIFIKDGRHPVVEKGIDNFVPNDTYMNCSTDKFLIITGPNMAGKSTYIRQV
                     GLIVIMSQSGCFVPASFAKIGIIDKIFTRIGSQDEISKGQSTFMVEMTETAEIINNLT
                     ERSLIILDEIGRGTSTYDGFSLALSISEYLQSKKVKVLFATHFHELTVLSQKYKGVKN
                     YNVSVRKEGENVIFLHKIMEGSSDESYGIYVAKLAGIPEKIIKRADEILRLLEAEDKI
                     KAQIMEKSEIAIPSLFENVDLYKEKNNKEEMDLKKEIKNMDIENITPLQALLKLKELK
                     EKIDKS"
     gene            3018..4718
                     /gene="mutL"
                     /locus_tag="PLW95_07840"
     CDS             3018..4718
                     /gene="mutL"
                     /locus_tag="PLW95_07840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOV22565.1"
                     /translation="MGKINVLPEKVISKIAAGEVVERPSSVLKELLENSIDAKSSKIF
                     VEIEKAGEKLIKVVDNGVGIDQEDIGYVFSRYATSKIKDEKDLEKILSLGFRGEALYS
                     IGVVSDVILRSRSKGDEIGTEIHIRGGKELSRKKVGMSEGTIVEVRELFFNTPVRKKF
                     LKSDTTELRQILNVFLPYAILFYNVEFKLIHNKREIFYLPTTSDLKERVKSATGINSD
                     ELILIEKKFDNFSVNFLLGNINIQRPRRDLQFLYVNKRPVYNFNILNSINRVYRAILP
                     PDVYPAFFIMIEIDPEMVDVNIHPTKREIKIKNENEIIKLIGEEIYYTLVEKGKGKKV
                     ERKSVYIERKEEGIEPQPIKERGIFDFKTEDREETKEKDNLKNKLKDITFIGVLKNKY
                     IFFQGEETLFVFDQHAVHERINYEKFLAQIKSKNIEIQKLLTPIIVKLTKEEMVIYEE
                     IKEKIENCGFLTTRWSEDEVAIHGYPTFLKNPEFSLRTIFTDIVSPQGPSAKIIDEEK
                     IARSACRSSIMAGDKISEGECLSLIKQLLKCENPFVCPHGRPTVIEFPISFFDRQFLR
                     "
BASE COUNT         1904 a          539 c          899 g         1495 t
ORIGIN      
        1 ttttttcttg ctactctctc cttgctactt tttaagaact gcttgctact tttacaaagg
       61 ggttaaagga aaggaaatgt ggtataattt tcttatggag aatttaaccc ctatgttgga
      121 gcagtataat tcaataaaaa aggaatatcc tgattgtatt gtttttttcc gattaggcga
      181 tttttatgag atgttttatg atgatgcaaa aattgcctct caaattcttg accttgtttt
      241 gacttcaaga agtgcaggca aaatgggtaa agtgcctatg tgtggtattc ctttccattc
      301 agccgactcc tatatttcaa aattagttaa agccggatat aaagttgcta tttgtgaaca
      361 ggttgaggac ccatcaaaag caaaaggtat agtaaaaaga gaggttataa gggttataac
      421 ttctgggact tttattgatg aaaattcaac agaaggaagg tatttagttt caatttaccc
      481 cgataaaaaa acaataggta tttctttttt gagttcagaa ggagaaacaa tttttacaaa
      541 tcaatttgat gatataacaa aagtggttga tattatattt aaatacccta tttatgagat
      601 tattttccca gaagaaaaag aggaatttat aaaaaatata tttgaacaat atccattttt
      661 aaagttaaga aaaattgttt tgagtagttg caatgactat actttcaatt atgaaatttc
      721 aaaggagaca attttttcgc attttaaggt tgagagtttg cgaggatttg gaattgaaga
      781 gaaagaactt tgtgtaagaa cttcaggagc aataattgaa tatttaaagt ttgttaataa
      841 aaaagatttt tcccatctaa acaaaatttc aatttattct gatgatgatt ttctttatat
      901 ttctccttct gctttttatg gacttgaaat aaataaattg gtaggaattt tgaatatagc
      961 agttactcca tctggaaaac gacttctaaa ggactggatt taccatccat tgaaaaaggt
     1021 agaagaagta aaagaaagac ataaagcagt aaggttttta aaagaaaaac aagagataag
     1081 agaaaaggca agaaatttat taaaaggaat tggggatgtt gaaaaagcag tctcaaaaat
     1141 aagttgtgga tatggaaatg ttaaggatat ttttattgtt gctaatttaa tttttaaaat
     1201 ccctgatata aaaaaagtta tttcagaaat tgatattaaa aataattatt tttcagttga
     1261 agatagtgat gatttaagaa attttcttga tatggctata aattttgata tttctcctat
     1321 aaattatgaa ggaaaactta taaaagaagg atataatatt tctcttgatg aattaagaga
     1381 aataagttat aaggcaaaag agtatttaag aaatttacaa aaaaaggaaa tagaaaaaac
     1441 aggtataaat tccttaaaga ttgggtttaa taaggtcttt ggttattata ttgaaattac
     1501 aaaggcaaat ttacatcttg tcccttccga ttatataaga aaacaaacgc ttgtaaatgc
     1561 tgaaaggttt ataaccccag aacttaaaga gtttgaagag aaaattttaa ctgctgatac
     1621 aaaaattaaa gaaattgaag ataaaataat tgaagaagtt aagaataaaa taattgaaaa
     1681 tgctccaacc atttattcaa tcacaaaaaa aataagtgaa ttagatgttt taatatgttt
     1741 tgcatctgta tcagaagaaa ataattatgt aatgccagaa attgatgagg ggtttgagat
     1801 ttttataaaa gatggaaggc atcctgttgt tgaaaaggga attgataatt ttgttcccaa
     1861 tgatacttat atgaattgtt caacagataa gtttttgata ataacaggtc caaatatggc
     1921 aggaaaatca acttatataa gacaggttgg acttattgtt attatgtctc aaagcggttg
     1981 ttttgttcca gcgagttttg cgaaaattgg tataattgat aaaattttta caagaattgg
     2041 ttcacaggat gaaataagta aagggcagag tacttttatg gtagagatga ctgaaacagc
     2101 agaaataata aacaatttaa cagaaaggag tttaataata cttgatgaaa ttggaagagg
     2161 gacaagcact tatgatggtt tttctcttgc attatctatt tcagagtatt tacaaagtaa
     2221 aaaagtaaag gttctttttg caacacattt tcatgaatta acggttcttt cccaaaaata
     2281 taaaggggta aagaattata atgtttcagt tagaaaagaa ggagaaaatg taatttttct
     2341 tcataaaata atggaaggta gttctgatga aagttatgga atttatgttg ccaaacttgc
     2401 aggaatacct gaaaaaataa taaaaagggc tgatgaaatt ttacgactac ttgaagcaga
     2461 agacaaaatc aaggcacaga taatggaaaa aagtgaaatt gctattccat ctctttttga
     2521 aaatgttgac ctgtataaag agaaaaacaa taaggaagag atggacttga agaaagaaat
     2581 aaaaaatatg gatattgaaa atattacacc tcttcaagca cttttaaaac ttaaagaact
     2641 aaaagagaaa attgataagt catgaacaaa tcccattagg gatattaata aaaagtaaaa
     2701 agtccccctc accccaaccc tctccccagc ggggagaggg aaatcccccc caattccccc
     2761 ctttattaaa ccgactgggg aagcaaaggg tagcagggca gagttggtgc ttaatgtcta
     2821 agcacaacgg gggttagagg gaattttgga gggggattta gcaagcacaa cggggagtaa
     2881 ggggagtgag ggggatttaa taaaatccac ccctgccctc ctttacaaag gaaggagaaa
     2941 aaagagaggg gaaggtaggg ggattttaag acttcgtgaa atataaacgg ataataaata
     3001 tttgaggaaa attgaaaatg ggaaagatta atgtattacc tgaaaaagta ataagcaaaa
     3061 tagcagcagg agaagttgta gaaagacctt cttctgtttt aaaagagttg cttgaaaatt
     3121 ctattgatgc aaaaagtagt aaaatttttg ttgagataga aaaggcagga gaaaaactga
     3181 ttaaagttgt tgataatggg gttggaattg accaagaaga tattggatat gttttttcaa
     3241 gatatgcaac aagtaaaata aaagatgaaa aagaccttga aaaaatcctt tcattgggtt
     3301 ttagagggga ggcactttat agtattgggg ttgtttcaga tgttatttta agaagtagaa
     3361 gtaaaggaga tgaaataggg actgaaatac atataagagg tggaaaagaa ttaagtagaa
     3421 aaaaagtagg aatgagtgaa ggaacaattg ttgaagtaag agaactattt tttaacaccc
     3481 ctgttagaaa aaaattctta aaatcagata caactgaatt aagacagatt ttaaatgttt
     3541 ttttacctta tgctattctt ttttacaatg ttgaatttaa actgattcat aacaaaagag
     3601 aaatttttta tttaccgaca acttccgatt taaaagaaag agtaaaatca gcaacaggaa
     3661 taaattcaga tgaactaatt ttaatagaaa aaaaatttga taatttttct gtaaattttt
     3721 tactcgggaa tataaatata caaagaccaa gaagggattt acaatttctt tatgtaaata
     3781 aaaggcctgt ttataatttt aacattttaa attccataaa cagggtatac agagccattt
     3841 tacctcctga tgtatatcct gcttttttta taatgataga aatagaccca gaaatggttg
     3901 atgtaaatat ccacccaaca aaaagagaaa taaaaataaa aaatgaaaat gaaattataa
     3961 agttaatcgg agaggaaatt tattatactc ttgtagaaaa aggaaaaggg aaaaaagttg
     4021 aaagaaagtc cgtttatatt gaaagaaaag aagaaggtat tgaaccccag ccaataaaag
     4081 aaagggggat ttttgatttt aagacagaag atagagaaga gacaaaggaa aaggataatt
     4141 taaaaaataa gttaaaagac ataactttta ttggtgtttt gaaaaataaa tatatctttt
     4201 ttcaaggtga agagaccctt tttgtttttg accagcatgc agtccatgaa agaataaatt
     4261 atgaaaagtt tttagcccag attaaaagta aaaatattga aattcaaaag ttattaacac
     4321 caattatagt aaaattaaca aaagaagaga tggttattta tgaagaaata aaagagaaaa
     4381 ttgagaattg tggatttttg accaccagat ggagtgaaga tgaagttgca atacacgggt
     4441 atcctacatt tttaaaaaat cctgaattct ctctcagaac aatttttact gatattgttt
     4501 ctccacaggg tccttcggcg aaaattattg atgaagaaaa aattgcaagg agtgcctgta
     4561 gaagttcaat tatggcagga gataaaattt cagagggaga gtgcctttct cttataaaac
     4621 aattacttaa atgtgaaaat ccttttgtct gtccacatgg cagaccaaca gttattgaat
     4681 ttcctataag tttttttgat aggcaatttt taagataaat cagtcaaaac agagaaattg
     4741 aagtttagaa attggtaaaa attttcccac aggagattgt caaaataact tttcctttaa
     4801 gtttccatcc aataaatggg gaatttttac tttttga
//
LOCUS       DAOOVW010000078         4804 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_9871_length_4804_cov_9.310381, whole genome shotgun sequence.
ACCESSION   DAOOVW010000078 DAOOVW010000000
VERSION     DAOOVW010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9871_length_4804_cov_9.310381"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="PLW95_07845"
     CDS             complement(<1..582)
                     /locus_tag="PLW95_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018194332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HOV22566.1"
                     /translation="MKGKNESIIKRFFFERNPDIVAKELLGKKFIRKICGKILSGIIV
                     ETEAYFGENDPASRAYKRKSKLFHKKMSSKAGTLLIYVVHNNYLLNIISHKKNKVGAV
                     LIRAVEPIEGIEIMKKNRGKEKIEDLSSGPGKFTKCFCIDKKFDGIDITKSKNEIYIL
                     NTENKNFEIKSSKRIGVKTDLKRNLRFFISGNKF"
     gene            615..1208
                     /locus_tag="PLW95_07850"
     CDS             615..1208
                     /locus_tag="PLW95_07850"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HOV22567.1"
                     /translation="MKEITKKWDFIWENYYEKLEYLSLAKKISISLLFALIIGISGQI
                     YIKLPFTPVPLTLQVFFVLLSGILLGSNFSALSNIFYLLFGILGINWFFASSSGFFRP
                     TTGYIIGFIFSSYFVGKYFFFRKSKIYSFLIMFSAVLIIYIFGCLFLSVFLKFNFKKT
                     ILLGVLPFIPFDLVKAYLAGIIGNSIMRNKRRNYERI"
     gene            1195..2211
                     /locus_tag="PLW95_07855"
     CDS             1195..2211
                     /locus_tag="PLW95_07855"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007956427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOV22568.1"
                     /translation="MKEYNVAVIGIGMVGKKMVEILYQRNFPLKNLKVLATREREEEI
                     AGKILKIEKIKENSFNEIDIAFFAGTEGEKGASSLFGWEAVKKGCIVIDNGADFRMDE
                     KVPLIIPEVNPHHLKKHQGFIANPNCSTIQMVMAIYPLDKEFCLKRVIVSTYQAVSGT
                     GRKAVEELKKQTESFVKGEKIKSEVYPHQIFGNLIPEIGSLSDEFPGYFTEEVKMIKE
                     TRKILDNYDLKISATCVRVPVFNGHSECITCEFEKEVHVKKVREILSNFHGIKVMDRP
                     EESYYPTPLFATEKDEVFIGRIRKNPAIENAIDMWVVSDNIRKGAALNTIQIAEKMIE
                     MSLL"
     gene            complement(2297..4555)
                     /locus_tag="PLW95_07860"
     CDS             complement(2297..4555)
                     /locus_tag="PLW95_07860"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HOV22569.1"
                     /translation="MNKNVYFPKELEWMWNARYSSWLFTYDQVFNYSVNDFEKKAKAL
                     HNGGINTVIIAGFHFRWDWYYLWPELTQALVRIVNACHKYGIRVIEHHSSVLKSFYPN
                     KEELEIYKKKFDLNRYPWFSQHIKDVTCDPVIDNIPFSSMYQVDIRTGEPSWSYYHGH
                     IFCFNNPDYQRIYFRYLEDLYRTGIDGIMTDDIQFMPCIYSCGCYWCRKLFTEQHGYE
                     IPEKDTEDRSFFFNFDNPAFRAFIRFRSNSHIAHHHRVNEHFQKLGYIMARPNYCSAS
                     TSVFGVVGTGYDLEGAAPCFNTVFTEICHSEEQLSCWAWTVHELKHKSALAARYRIPA
                     MALFYSANQSQGYFHWALSKILGQNLWLSGGSGSSIDFNAHKEALLSKQGNVFQQQHP
                     KLYIHPVSCARVAVFFSRNTMMNYQAASGESFTNEYAGWCQQLFKNNILFDVILDDDI
                     EDISVLKKYDLIILPNTACMSDVQTDNITQFVKEGGGLIATFETGHYNQTGEHRKIPA
                     LQELFGIKDLGLIPSQNFWKAGPDVSLLNVQIPFYIDNCRPYRQVKPTRDGKIILESV
                     NEDLWPNSPVYFKTSFGRGNITYFSGYIGSQSFMPFGHAISTEKDRNDRSTKYLALKK
                     ASDPCLAQILVQSVRNMLGEKQQLLIEGAPEGILAGLYQHGEEYVLHLLNASKTLRRQ
                     GETFGGNGKWNAKPCGEIHITLRFCPCRTATLFSPDFEGSYQVPVKISEDTLNLTIKA
                     GLLQKYSVINLK"
BASE COUNT         1582 a          761 c          779 g         1682 t
ORIGIN      
        1 aaatttgttt ccacttataa aaaatcttaa atttcttttt aagtcagttt ttactcctat
       61 tctttttgaa ctttttattt caaaattttt attttctgta tttagaatat aaatttcatt
      121 tttactcttt gttatatcaa tcccatcaaa ttttttatca atacagaagc attttgtaaa
      181 ttttcctgga cctgatgata ggtcctctat tttttctttt cccctatttt ttttcattat
      241 ttcaattcct tctattggtt caactgccct tattaagaca gcaccaactt tatttttttt
      301 gtgagagata atatttaaaa ggtaattatt atgaacaaca taaattagta atgttcccgc
      361 ctttgatgac atttttttgt gaaataattt actttttctc ttatatgctc tacttgccgg
      421 gtcattttca ccaaaatatg cctctgtttc tactataatc ccggacaaaa tttttccaca
      481 tatttttcta ataaattttt ttcctaataa ttcttttgct acgatatctg gatttctttc
      541 aaaaaaaaac cttttaataa tactttcatt ttttcctttc atattgtaaa attataaact
      601 aaacaggaga gaaaatgaaa gaaataacaa aaaaatggga ttttatctgg gaaaattatt
      661 atgaaaaatt ggagtatcta tcattggcaa aaaaaatatc tatttctttg ctttttgcac
      721 ttataatagg tatttcaggg caaatttaca taaaattacc ttttacacca gttccattga
      781 cattacaggt attttttgtt ttactttcag ggattttgct tggaagtaat ttttctgccc
      841 tttctaatat tttttattta ctttttggta ttttagggat taactggttt tttgccagta
      901 gttctggttt ttttagacct acaacaggat atataattgg ttttattttt tcttcatatt
      961 ttgttgggaa atatttcttt tttagaaaaa gtaaaattta ttctttttta attatgtttt
     1021 ctgctgtttt aattatttat atttttggtt gtctatttct ttcagttttc cttaaattta
     1081 attttaaaaa gacaatactt cttggtgttt taccttttat tccttttgat ttagttaagg
     1141 catatctggc aggtataatt ggaaattcca taatgagaaa taaaaggaga aattatgaaa
     1201 gaatataatg ttgcagttat tggtattgga atggttggta aaaaaatggt tgaaatactt
     1261 tatcaaagaa attttccatt aaaaaattta aaagttcttg caacaagaga aagagaagaa
     1321 gaaatagcag gaaaaatttt aaagattgaa aaaataaaag aaaattcatt taatgaaata
     1381 gatattgctt ttttcgcagg aacagaaggg gaaaaaggag caagttcttt atttggatgg
     1441 gaagcagtga aaaagggctg tattgttatt gataatgggg cagattttag aatggatgaa
     1501 aaagtcccct tgataattcc agaagtaaat ccacatcatt taaaaaaaca tcagggattt
     1561 attgcaaatc caaattgttc aactattcaa atggtcatgg caatttatcc tcttgataaa
     1621 gaattttgtt tgaaaagagt tattgtttca acatatcagg cagtttcagg aacaggaaga
     1681 aaggcagttg aagaattaaa aaaacaaaca gagagttttg taaaaggaga gaaaataaag
     1741 agtgaagttt atccacacca gatttttggc aatttaatac cagaaattgg cagtttatca
     1801 gatgaatttc ctggctattt tacagaagag gttaaaatga taaaagaaac aagaaaaatt
     1861 cttgacaatt atgacctaaa aatttccgca acatgtgtta gagtacctgt gtttaatggt
     1921 cattcagaat gtattacctg tgaatttgaa aaagaagtac atgtaaaaaa agttagagag
     1981 attttaagta attttcatgg tattaaagtt atggatagac cagaggagtc atattatcca
     2041 acacctttat ttgcaaccga aaaagatgaa gtatttattg gaagaataag aaaaaatcct
     2101 gcaattgaaa atgcaataga tatgtgggtt gtttctgaca atataagaaa aggtgctgct
     2161 ttaaatacaa tacaaattgc tgaaaaaatg atagaaatgt cccttctata atattcagta
     2221 aatatatctt atgtaaatta agcacaatat ctactctctt catcatactt taattaattt
     2281 ttcattggat tactttctat ttcagattaa ttactgagta tttctgtagt aaacctgctt
     2341 taattgttag gtttaatgta tcttctgaaa ttttcacagg cacctgataa cttccttcaa
     2401 aatcaggaga gaaaagtgtg gctgtccggc acggacagaa acgcagagtt atgtgaattt
     2461 ctccacatgg ttttgcattc cactttccgt ttcctccaaa tgtctctcct tgtcggcgca
     2521 atgtttttga tgcgttaaga aggtgaagaa catattcttc accgtgttga tataatcctg
     2581 ccaaaatacc ctcaggtgct ccttcaataa gtaattgttg tttttcacca agcatatttc
     2641 tgactgactg cacaagaatc tgtgccagac aaggatcgct tgcttttttg agggcaagat
     2701 atttagtact gcggtcatta cggtcttttt ccgtagatat agcatgtccg aaaggcataa
     2761 atgattggct accaatatat ccagaaaaat aagtaatatt tccccttcca aaagaagttt
     2821 tgaaatacac aggagaattt ggccataaat cttcatttac actctcaaga attatcttcc
     2881 cgtcccgagt tggcttaacc tggcggtatg gtctgcagtt atcaatataa aaaggaattt
     2941 gtacatttag gagagaaaca tctggtcctg ctttccaaaa attctgcgat ggaataagcc
     3001 caagatcctt aataccaaaa agttcttgta aagcgggtat ttttctatgt tcacctgtct
     3061 ggttgtaatg acctgtttca aatgtagcaa taagtccacc accttctttt acaaactgcg
     3121 ttatattatc agtttgtaca tcactcatac aggcagtatt gggaagaata ataaggtcat
     3181 attttttgag aactgatata tcttcaatat catcatcaag aattacatca aaaaggatgt
     3241 tatttttaaa gagttgttga caccaccctg catattcgtt ggtaaaagat tctccactcg
     3301 cggcttgata gttcatcatt gtatttcggg agaaaaacac cgcaactctt gcacaagaaa
     3361 caggatgtat ataaagtttt ggatgttgtt gctggaaaac attaccctgt tttgaaagta
     3421 atgcttcttt atgagcatta aaatcgatag aactacctga accacctgaa agccagagat
     3481 tctgacccag aatttttgat aaagcccagt ggaaatatcc ctgtgactga tttgcactat
     3541 aaaaaagtgc catagcagga atacggtagc gtgcagcaag agcagatttg tgtttgagtt
     3601 catgcactgt ccacgcccag caacttagtt gttcttctga gtgacagatt tcagtaaaaa
     3661 cggtgttgaa acagggtgca gcaccctcaa ggtcgtatcc agttcctaca acaccaaata
     3721 cagatgtaga ggcagagcaa tagtttggtc tagccataat atacccaagt ttctggaaat
     3781 gttcattgac tctatggtga tgagcaatat gtgagtttga gcggaagcgg ataaatgcgc
     3841 gaaaagcagg gttgtcaaaa ttgaagaaaa atgacctgtc ttctgtgtct ttctccggta
     3901 tttcatagcc gtgctgttct gtaaaaagtt ttctacacca gtagcaacca caagaataaa
     3961 tacaaggcat gaattgaata tcatctgtca taataccatc tataccagtg cggtataaat
     4021 cttcaagata cctgaaataa attctttgat aatcagggtt attgaaacag aaaatatgtc
     4081 catggtaata actccaactt ggttctccag ttctaatatc cacctgatac atagaagaaa
     4141 atggaatatt atctatcaca gggtcacagg tcacatcttt gatatgttga ctgaaccatg
     4201 gatagcgatt aagatcaaac ttctttttgt atatttctaa ttcttccttg tttgggtaaa
     4261 aacttttcag tacactactg tggtgttcaa taactctaat gccatattta tgacatgcgt
     4321 tcactatgcg gacaagtgcc tgtgtcagtt caggccagag ataataccag tcccatctga
     4381 aatgaaaccc agcaataatt actgtgttaa tacctccatt atgaagtgcc tttgcttttt
     4441 tctcaaaatc atttacagaa taattaaaaa cctggtcata agtaaatagc cacgaactat
     4501 aacgggcatt ccacatccac tcaagttctt ttgggaaata aacatttttg ttcataataa
     4561 cattctcctt gctaaaatta ctctccattt ttctctgtgt aaatatttac tcaaccccat
     4621 ctttctgatg agaatattta tctactttta tactatatta aaaggagagt taaagttaaa
     4681 attattttat caattaaact ttaacatatc tcctatcaat tgacaagtca acttcgccat
     4741 ttcattttgg gcaattatga aaacacttgt aaataaaggg gttaaagaga ttttaatttg
     4801 ttca
//
LOCUS       DAOOVW010000079         4681 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_10175_length_4681_cov_7.477086, whole genome shotgun sequence.
ACCESSION   DAOOVW010000079 DAOOVW010000000
VERSION     DAOOVW010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4681
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10175_length_4681_cov_7.477086"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..1815
                     /locus_tag="PLW95_07865"
     CDS             67..1815
                     /locus_tag="PLW95_07865"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOV22570.1"
                     /translation="MKTKKLEKTNFKNRNFINLESISLIIFFFAGILLNFKPAYDYTI
                     IKVVFGYLFVLLLSTSYILEKKEFHFNYSILIIFLIFFSYILFSSFTAPFKYGSAFLL
                     ENYLLYFLIFVLSLNLEIKNEWIYYWLIAAFISSITGLFQYLGPRHYAISTFGNPNFF
                     AGHIIMPICIGISLLFSKEFKKPQKFLTLFFVIICFITLILTRSRAAIVGFLFGISTI
                     FFLINKNKKIKWLGYIILLLISVFLSKFIYKEFLTNIRYYIWTGTIKLIKQKPITGWG
                     FGQFIFYYFYFRRRDYFLQSESTPVTNHAHNEYLEMLSEIGIIGILIFFIFIYIIVNK
                     FFKSKELCGKNYKLEKNLIAGFVGGIFAVMVDNILSTNLRNPSTAMYFYFLLGILGSL
                     RRNRAFHFNISKLLYSTTFFVSFLMIILTSYYRILPDVYLKRGIWAKDMGDYKKAIEN
                     YKVVCSLNPYNYTALYKLAFVYGEIGDYKNAERIYLYINNYVFPHFAKTDTNLGTVYL
                     KIGDYEKALKYYLYAEWLNPYDKDVLCSISSIYLIYYNDVDKALKYLNKVLTIDPENR
                     YANTVKSQLKKEGKIK"
     gene            1825..2043
                     /gene="rpmB"
                     /locus_tag="PLW95_07870"
     CDS             1825..2043
                     /gene="rpmB"
                     /locus_tag="PLW95_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOV22571.1"
                     /translation="MSKVCEICGKRSKTGNNVSHSNKKTRRVWKPNIQCFSVEIDGVR
                     RKINICTRCLRSGKVKKAVNRKSLNVEI"
     gene            complement(2064..3611)
                     /locus_tag="PLW95_07875"
     CDS             complement(2064..3611)
                     /locus_tag="PLW95_07875"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOV22572.1"
                     /translation="MEKIINKILENRGIKEKEAFFSSEISSPVDPFVVPGIKQACEII
                     KRIIEKKENVFVYGDGDVDGIFGTYFIIKMFEKTGTPFSYYLTHRLDTYEIEEEFISY
                     LKKENISLFITVDCGISSVNFLKKMEDEKIKVIVIDHHIFDIFFLPKTHTYINPKLYN
                     TATEIKYLSSTALAQKLSNALSFFIPFPFEENIELVALSTIADNCPVIGENRKLIMTG
                     LDKIFSTEIIGLASLIDELKIKNKNLINQLKMKLIPKLNSPGRFGKPEIVLDLLLSEN
                     VDKIIKEIKEIDRKRYQLTKIHLTKFREKAEKATFLVESEILPTICGIISSRLSYEMN
                     KPIAIASERNGIIQGSARCPELFNLFDFIKKTKNYMLSSGGHQQAIGFTFDKKFLNNI
                     KEEWENFLFTGKEENFYYDAEIELVNLTPVFLEELSKFAPFGPGNPEVVFLSKNIKVE
                     KIMRKTDEELTFWVRENNSLFECITKNFNGNKEILEGNFINIYYIPKYRENNGLYRIY
                     LLVKKVE"
     gene            complement(3583..4074)
                     /locus_tag="PLW95_07880"
     CDS             complement(3583..4074)
                     /locus_tag="PLW95_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HOV22573.1"
                     /translation="MNSQYTQIHRPDWDHYFMKIAELVSERSTCLRRKVGCVLVKRKR
                     ILATGYNGAPTGLPHCEEVGCIRVELKIPSGERHELCRGLHAEQNAIIQSAYYGVSIK
                     DSILYVTCHPCSVCAKMIINSGIKEVIIKEGYPDKLATDFFKNANVKVRYIENGKNNK
                     QNT"
     gene            complement(4046..>4681)
                     /locus_tag="PLW95_07885"
     CDS             complement(4046..>4681)
                     /locus_tag="PLW95_07885"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548053.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOV22574.1"
                     /translation="GLVIAGIHQSPFPFTDFVTTTTHKTLRGPRGGLILCKNIFKENI
                     DSTIIPGIQGGPLVHVIAGKAVAFKEASEKSFIDYQKQIVKNSKKLCEELKKRDYRIV
                     SNGTDNHLMVVDLRNKGLTGLEGQEILEKGGIITNKNLIPFDPLPPAKTSGIRIGTPA
                     ITSRGMKEKEIEKIADWIDIILSNSKNEKILKDISKKIRRFSNEFPIYPNT"
BASE COUNT         1546 a          638 c          617 g         1880 t
ORIGIN      
        1 atatccccac atagtgtaaa attataccat atcgctataa ttgtgtaaat aaaactctaa
       61 ttgaaaatga aaacaaaaaa attagaaaaa acgaatttta aaaataggaa ttttataaat
      121 ttagaaagca tctcgttaat tatttttttc tttgctggaa tacttctaaa ttttaaacct
      181 gcttatgatt atacaattat aaaggtagtt ttcggttacc tttttgtttt attgctttca
      241 acaagttata tattagaaaa aaaggaattt cattttaatt attcaatttt aattatattt
      301 ttaatatttt tttcttatat tttgttttct tctttcactg ctccttttaa gtatggcagt
      361 gcctttcttc ttgaaaatta tcttctatat ttccttatat ttgttttatc tctgaattta
      421 gaaattaaaa atgaatggat atattattgg ttaattgcag catttatttc ttcaatcact
      481 ggtttatttc agtatttagg tcccagacat tatgctattt ctacatttgg taatcccaac
      541 ttttttgcag gtcatattat aatgccaatt tgtattggta tttctttact tttttctaaa
      601 gaattcaaga aaccacaaaa atttttaact ttattttttg ttattatatg ctttattact
      661 ttaattttaa caaggtcaag agcagcaata gtaggatttc tttttggtat atcaactatt
      721 ttttttctca taaataagaa taaaaaaatt aaatggcttg gatatattat tttgcttctt
      781 atttctgttt tcctttcaaa atttatttat aaggaatttt taacaaatat aaggtattac
      841 atatggacag gaactataaa acttataaaa caaaaaccaa taactggctg gggttttgga
      901 caatttattt tttattattt ctattttagg agaagggatt actttcttca gtcagaatca
      961 actcctgtaa ctaatcatgc tcataatgaa tatcttgaaa tgttatccga aataggaata
     1021 ataggtattc taatcttctt catttttatt tatataattg ttaataaatt ttttaaatcc
     1081 aaagagttat gtggaaagaa ttacaaatta gaaaaaaatt taattgctgg ttttgttggt
     1141 ggtatctttg ctgttatggt tgataatatt ttatcaacaa atttaagaaa tccttcaact
     1201 gctatgtatt tttatttttt attgggtatt ttaggttctt tgaggagaaa cagagcattt
     1261 cattttaata tttcaaaatt attgtattct actacttttt ttgtgtcttt tttgatgatt
     1321 atacttacct catattatag aattttaccc gatgtctatt taaaaagggg aatttgggca
     1381 aaagacatgg gtgattataa aaaggcaatt gaaaattata aagttgtttg ttcattaaat
     1441 ccatataatt atactgctct gtataaactt gcttttgtat atggagaaat tggtgattat
     1501 aaaaacgcag aaagaattta tctatatatt aacaattatg tttttccaca ttttgctaaa
     1561 acagatacaa atttagggac agtttatctt aaaataggtg attatgaaaa agcattaaaa
     1621 tattatcttt atgctgagtg gttaaatcct tatgataaag atgttctttg tagtatatct
     1681 tctatttatt taatttatta taatgatgtt gacaaagcgc tcaaatattt aaataaggtc
     1741 ttgacaattg acccagaaaa taggtatgct aatacagtta aaagtcaatt aaaaaaagaa
     1801 gggaaaatta aataggagat agggatgagt aaagtttgtg aaatatgtgg aaaaaggagt
     1861 aaaacaggaa ataatgtaag tcattctaat aaaaaaacca gaagagtgtg gaaaccaaat
     1921 attcaatgtt tttccgttga aatagatgga gtgagaagga aaataaatat ctgtacgaga
     1981 tgtttaagaa gtggtaaagt caaaaaagca gttaatagga agagtttaaa tgtggagatt
     2041 taacccctgc ggttagaaaa aaattattca acttttttta ccagcagata tattctgtaa
     2101 agaccgttgt tttctctata ttttggtatg taataaatat ttataaaatt accttcaagt
     2161 atttctttat ttccattaaa gtttttagtt atacattcaa atagagaatt attttctcta
     2221 acccaaaaag ttaattcctc atctgttttt ctcattattt tttcaacttt tatattttta
     2281 gataaaaaaa ctacctctgg atttccagga ccaaaaggag caaattttga taattcttca
     2341 agaaaaacag gagttaaatt aacaagttca atttcagcat cataataaaa attctcttct
     2401 tttccagtga ataagaaatt ttcccattct tcttttatat tgtttaagaa ttttttatca
     2461 aatgtaaaac caattgcttg ttgatgtcca ccagaagaaa gcatataatt ttttgttttt
     2521 ttgataaaat caaaaagatt aaagagctca ggacatcttg ctgaaccctg tattattcca
     2581 tttctttcag aggcaattgc aattggttta ttcatttcat aagataatct tgaagatatt
     2641 attccacaaa tagttggtaa tatctcactt tcaacaagaa atgttgcttt ttctgctttt
     2701 tctctaaatt tagttaaatg aattttagtt aattggtatc tttttctatc tatttcctta
     2761 atctctttta ttattttatc tacattttct gataaaagta aatcaagaac aatttctggt
     2821 ttaccaaatc ttcctggtga attcaatttt gggatgagtt tcattttgag ttggtttatt
     2881 aaatttttat tttttatttt caattcatca attaatgatg ccaatccaat tatctcagta
     2941 gaaaaaattt tatcaagtcc tgtcattata agttttctat tctctcctat aacaggacaa
     3001 ttatcagcaa ttgttgataa agcaactaac tcaatattct cttcaaaagg aaaagggata
     3061 aagaaactta atgcatttga aagtttttga gcaagagcag ttgaacttaa atattttatt
     3121 tctgtggctg tgttataaag ttttggattt atataggtat gtgtttttgg gagaaaaaat
     3181 atatcaaaaa tatgatggtc tattacaatt acttttattt tttcatcttc cattttttta
     3241 aggaagttca cagaagatat tccacaatca acagtaataa aaagagaaat gttttctttt
     3301 tttaagtaag aaataaattc ttcttcaatt tcatatgtat caaggcgatg tgtaagataa
     3361 taagaaaagg gggtccctgt tttttcaaac atttttataa taaaataggt gccaaaaatt
     3421 ccatcaacat ctccatctcc atatacaaaa acattctctt ttttttcaat aattcttttt
     3481 attatttcgc atgcttgttt tatacctggt actacaaaag ggtcaacagg tgaggaaatt
     3541 tcagatgaaa aaaatgcctc tttttctttt atacccctgt tttcaagtat tttgtttatt
     3601 attttttcca ttttctatat accttacctt aacattcgca tttttaaaaa agtcagttgc
     3661 aagtttatca ggatatcctt ctttaattat aacttcttta attcctgagt taataatcat
     3721 ttttgcacaa acagaacacg gatgacatgt tacatataaa atactatctt taattgagac
     3781 accataatat gctgattgaa ttattgcgtt ttgttctgca tggagtcctc tacaaagttc
     3841 atgcctttct ccagatggaa tttttaattc aactcttata catccaactt cttcacaatg
     3901 aggaagtcct gttggtgcac cattataccc ggttgctaaa atcctttttc tctttactaa
     3961 aacacagcca acttttcttc ttaaacatgt tgacctctct gaaacaagtt ctgcgatttt
     4021 catgaaataa tggtcccaat caggtctatg tatttgggta tattgggaat tcatttgaaa
     4081 atctccttat tttttttgaa atatctttca atattttttc atttttacta tttgataaaa
     4141 ttatatcaat ccaatcagca attttttcta tttctttttc tttcatccct ctgcttgtaa
     4201 ttgcaggagt tccaattctt ataccacttg tttttgcagg aggtaggggg tcaaatggta
     4261 tgaggttttt atttgtaata ataccacctt tttcaagaat ttcctgacct tcaagtcctg
     4321 ttaagccctt atttcttaaa tcaactacca tcaaatgatt gtcagttcca ttacttacaa
     4381 ttctataatc tcgttttttt aattcttcac ataatttttt tgaattttta acaatttgtt
     4441 tttgataatc aataaaactt ttttcacttg cttccttaaa ggcaactgct tttcctgcta
     4501 ttacatgaac taatggtcct ccttgaattc caggtattat tgtgctatca atattttctt
     4561 taaatatatt tttacaaagt attaaaccac ctcttggtcc tcttaatgtt ttgtgtgttg
     4621 tggttgtaac aaagtcagta aatggaaatg gtgactgatg aataccagca attacaagac
     4681 c
//
LOCUS       DAOOVW010000080         4509 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_10628_length_4509_cov_10.790076, whole genome shotgun
            sequence.
ACCESSION   DAOOVW010000080 DAOOVW010000000
VERSION     DAOOVW010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10628_length_4509_cov_10.790076"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..503)
                     /locus_tag="PLW95_07890"
     CDS             complement(135..503)
                     /locus_tag="PLW95_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012965141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HOV22575.1"
                     /translation="MEKIKIKIGNIEVEGEILDTKLGKKIIEKIPFSSKANLWGDEIY
                     FEVDVDEKLEMPVSEVEKGDIGYWPTGKCFCLFFGPTPISYGDKIIPASKVEIVGKIT
                     KGIEKLKQVKERDEIIVEKG"
     gene            complement(469..975)
                     /locus_tag="PLW95_07895"
     CDS             complement(469..975)
                     /locus_tag="PLW95_07895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOV22576.1"
                     /translation="MEIKKFLKGKVVIIGIGNPLKGDDNAGNIIVKKLMEIVKSDKVN
                     FLVVEEGIENFIGKIERLKSDNIIIVDAVNFKGKIGEIKIFYPDQLMKNTTTTHSFSL
                     PDLLGIYNFKKCLIIGIQPGNLSFGTEISKEVKESVDKIVLLLAKYLTSQAGRRIYGK
                     NKNQNREY"
     gene            complement(1038..1634)
                     /locus_tag="PLW95_07900"
     CDS             complement(1038..1634)
                     /locus_tag="PLW95_07900"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HOV22577.1"
                     /translation="MKYNICEIFYSIQGEGKLQGFPFVFIRFSGCNLKCPWCDTKYAL
                     KKGYKIEIDEIIKKAGKYRCKNVCITGGEPYLQPLKPILEKLKENNFFVSIETNGTIW
                     QDIKFDWITVSPKKQGKKYHKNGYDEKFIKVASEFKYVITGKDDFKFIDKEIRKNIVL
                     QPVNNDKKISKLIIKYIKENPYLNYQIKLQLHKILKLP"
     gene            complement(1666..2775)
                     /locus_tag="PLW95_07905"
     CDS             complement(1666..2775)
                     /locus_tag="PLW95_07905"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOV22578.1"
                     /translation="MPGRELFGDEELKEVIDVMKRKVIFRYGFEKEREGIFKVVEFEN
                     EFSKFIGSKYSLGVSSGSSSLKVALESLNLPKGKEIITQCFTFSATIEAIRESGFIPV
                     LCEIDKTLNMDVDDLKRKINNRTVAIMPVHMMGVPSRIEEICEIAKDRNLKVVEDNCQ
                     STGALYKGKKLGTFGDIGCFSFDYVKVMTTGEGGMVVTDDEELYKQADYYHDHGHPHL
                     PGVSRGEEKKLRDGFNFRMNELQGAIGIAQLKKLPEVIRRHKENKNKIKNGIKNLNGI
                     EFREILDVDGDISTFLTMFLPDKEKAEKLKEKMRERGVAPATLNYWHFTANVEICGGN
                     YPKSQELLERSVVLELKAIMSDEEIEKIIDVMKSF"
     gene            2918..3325
                     /locus_tag="PLW95_07910"
     CDS             2918..3325
                     /locus_tag="PLW95_07910"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOV22579.1"
                     /translation="MKFKRHYQMTKGELNLTPLVDIIFQQIIYFMLTSSFIMQPGIKI
                     KLPKASTSTETIQKQEIYVSISEEGIIFYNGQPVKLEELEEIFRQEKERRQKLTLVIK
                     ADENTKHGRVVQVMDTGRKQGIDKIAVATTPEL"
     gene            3332..4018
                     /locus_tag="PLW95_07915"
     CDS             3332..4018
                     /locus_tag="PLW95_07915"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOV22580.1"
                     /translation="MEPNRLLLKTFTISLFFHIVGISLFSIIFPIPPSKTLPIEVTLL
                     PPAMDKKIQLETTEVLPNLPALETVSQSEKLAQSDNSSEIVKISEEKFTGFPEFIPTT
                     KITGEFEIPQFQVKFPPLPSIIQQAQSEIRTKSNEIIEGPIGERKLIYKEPIEYPDWA
                     EKNGIEGNIKIKFWVKADGKISETEIITSSGYPELDVYTMNKFMKYLFQPINKNEDVW
                     GIITFIFRLK"
     gene            4015..4248
                     /locus_tag="PLW95_07920"
     CDS             4015..4248
                     /locus_tag="PLW95_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22581.1"
                     /translation="MIKINIDIALTFYLMLPIIILTFWIFFEKRANKPFEIKSHDYLW
                     QCPICFYMYIDSKSDKISRCPRCKTLHKKGEKL"
     gene            4271..4477
                     /locus_tag="PLW95_07925"
     CDS             4271..4477
                     /locus_tag="PLW95_07925"
                     /inference="COORDINATES: protein motif:HMM:NF022229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOV22582.1"
                     /translation="MITEIGIVAGEIWSYLEKYNETTFEKLIKATGRDRDLLIMGLGW
                     LAREGHIILEKVGNDYKIKLHDKR"
BASE COUNT         1572 a          763 c          522 g         1652 t
ORIGIN      
        1 ggtggttcaa aatataatct gccaatatgc atatgaatat ctatcatttt ttctctcctt
       61 ccttaatttt caaggataat ttaaacattt tcccaatttt tgtcaaatta ctgaattagt
      121 tttgagccga atttttaccc tttttctaca attatttcat ctcgttcttt tacctgtttt
      181 aatttttcaa ttccttttgt aattttccca acaatctcaa ctttacttgc aggaataatt
      241 ttatctccat aacttattgg agttggccca aaaaacaaac agaaacattt accagttggc
      301 caatatccaa tatctccttt ctcaacttca cttacaggca tttctaattt ttcatcaaca
      361 tcaacttcaa aataaatttc atctccccat aagtttgctt ttgaactaaa tggaattttc
      421 tcaattattt ttttacctaa ttttgtatca agaatttctc cttctacttc aatattccct
      481 attttgattt ttattttttc catatatcct ccttcctgct tgactggtaa gatatttagc
      541 aagcaacaga acaattttat caacactttc cttaacttct ttacttattt cagttccaaa
      601 agataagttt cctggttgaa ttccaataat taaacatttt ttaaaattat atatacctaa
      661 aaggtcaggt aaagaaaaag aatgagtcgt agttgtattt ttcatcaact ggtcaggata
      721 aaatatttta atttctccta ttttcccttt aaaattaaca gcatcaacta taattatatt
      781 gtctgatttt aatctttcta ttttcccaat gaaattttca attccttctt caactactaa
      841 aaagtttact ttatctgatt taactatttc cataagtttt ttaacaataa tatttccagc
      901 attatcatca ccttttaaag gatttccaat tccaattatt acaactttcc cttttagaaa
      961 ttttttaatt tccattttat cataatacct taaaagaaat attatagata taataattcc
     1021 acagagtaaa gtgagattta tggaagtttg aggattttat gaagttgaag tttaatctgg
     1081 tagtttaaat atgggttttc tttaatatac ttaattatta acttactaat ttttttatcg
     1141 ttattaactg gttgaagtac tatatttttt cttatttcct tatcaataaa tttaaagtca
     1201 tctttccctg ttattacata cttaaattca gaggcaactt ttataaattt ctcatcatat
     1261 ccatttttgt gatatttttt tccctgtttt ttgggactta ctgttatcca gtcaaatttt
     1321 atatcctgcc atattgtccc atttgtttca attgagacaa aaaagttatt ttcttttaat
     1381 ttttccaaaa ttggctttaa tggttgtaaa tatggctctc cacctgtaat acacacattt
     1441 ttacacctat atttcccagc tttttttatt atttcatcaa tttctatttt atatcctttt
     1501 tttaacgcat attttgtatc acaccaagga cattttaaat tacacccaga aaaacggata
     1561 aaaacaaaag ggaatccctg taattttcct tcaccctgaa ttgaatagaa aatttcgcag
     1621 atattatatt tcacttcttt ttctatacca atatctccaa aataattaaa atgatttcat
     1681 aacatcaatt attttttcta tttcttcatc actcataatt gcttttaatt caagaacaac
     1741 gcttctctcc aaaagttctt gactttttgg atagtttcca ccacatattt caacatttgc
     1801 tgtaaaatgc cagtaattca aagttgccgg tgccactcct ctttctctca ttttctcttt
     1861 taatttttct gctttttctt tatctggcaa aaacattgtt agaaatgtag aaatatcacc
     1921 atcaacatca agtatttctc taaattcaat tccatttaaa ttttttattc cgttttttat
     1981 tttatttttg ttttctttat gtcttcttat tacttctggt aattttttta attgagcaat
     2041 acctattgct ccctgtaatt cgttcattct aaaattaaat ccatctctca gcttcttttc
     2101 ttcgcctctt gatacaccag gaagatgtgg atgcccatgg tcgtggtaat agtccgcctg
     2161 tttatataat tcttcatcat cagttacaac cataccaccc tcccctgttg tcataacttt
     2221 tacataatca aaagaaaaac atccaatatc accaaatgtt cccaattttt tacctttata
     2281 taatgcccct gtactctgac aattatcttc aacaactttt aaatttctgt cttttgctat
     2341 ttcacagatt tcttcaattc tggaaggtac ccccatcata tgaacaggca taattgcaac
     2401 ggttctgttg tttatttttc tttttaagtc atcaacatcc atattaaggg ttttatcaat
     2461 ttcacaaaga acaggaataa acccactttc ccttattgct tcaattgttg cagaaaatgt
     2521 gaaacactga gtaattattt ctttcccttt tggtaaattc aaactttcaa gtgcaacttt
     2581 taaagaagat gacccagaag aaacaccaag tgaatattta cttccaataa attttgaaaa
     2641 ttcattttca aattcaacta ctttaaaaat gccttctctt tctttttcaa atccatatct
     2701 gaaaataact ttccttttca taacatcaat aacttccttt aattcttcat caccaaataa
     2761 ttctctacct ggcattttca tctcctcctt acatttttat tttaatatta tattttattc
     2821 taaaatataa tttcttgcaa attccataag aaaatctaaa ttgcactata tctataaata
     2881 aactaaaata tataaaaaga aaaagaggaa tacaaaaatg aagttcaaaa gacattatca
     2941 aatgacaaaa ggagaattaa atttaactcc tcttgttgat ataattttcc agcaaattat
     3001 atattttatg ttgacttcaa gttttataat gcagccagga ataaaaatta aacttccaaa
     3061 agcatctaca tcaactgaaa caatccagaa acaagaaata tatgtaagta tatcagaaga
     3121 aggtattatt ttttataatg gacaaccagt aaaattagag gaattagagg aaattttcag
     3181 acaggaaaaa gaaaggaggc aaaaacttac tcttgttata aaagcagatg aaaatacaaa
     3241 acacggaagg gttgttcagg taatggatac aggaagaaaa caagggatag ataaaattgc
     3301 agtagcaaca actccagaac tttaatataa aatggaacct aatcgtctac ttcttaaaac
     3361 atttactata agtttatttt tccatattgt tggcatatca cttttttcta ttatatttcc
     3421 tattccccct tctaaaacat taccaattga agtaactctc cttccacctg caatggataa
     3481 gaaaattcaa ttagaaacaa cagaagttct cccaaatctt cctgctcttg aaacagtatc
     3541 tcagtcagaa aaacttgctc aatctgataa tagcagtgaa attgtaaaaa tttcagaaga
     3601 aaaatttact ggctttcctg aatttattcc aacaacaaaa attacaggtg aatttgaaat
     3661 acctcaattt caggtaaaat ttccgccact tccatcaata atacaacagg cacaatcaga
     3721 aataaggaca aaatcaaatg aaataataga aggaccaatt ggagaaagaa aacttatata
     3781 taaagaacca attgaatatc ctgattgggc agagaaaaac ggtattgaag gtaatataaa
     3841 aattaaattt tgggtaaaag cagatggaaa aataagtgaa acagaaatta ttacttcatc
     3901 aggatatcca gaacttgatg tatatacaat gaataaattt atgaaatatc ttttccagcc
     3961 aataaataaa aatgaagatg tctggggtat tataactttt attttccgac tgaaatgata
     4021 aaaataaata ttgatattgc tttaacattt tacttgatgt taccaataat aattcttact
     4081 ttctggatat tttttgaaaa aagggcaaat aaaccatttg aaattaaaag tcatgattat
     4141 ctatggcaat gtcctatatg cttttatatg tatattgaca gcaagtcaga taaaatatca
     4201 aggtgtccaa gatgtaaaac tcttcacaaa aaaggcgaaa aattgtaaaa taataaaaaa
     4261 tggaggaaaa atgataacag aaataggaat agtagcagga gaaatatgga gctatcttga
     4321 aaagtataat gagaccacat ttgaaaagtt aattaaagca acaggaagag atagggactt
     4381 actaataatg ggacttggct ggcttgcaag agaaggacat ataatactgg aaaaagttgg
     4441 caatgattat aaaattaaac tccacgataa aagatgatat ttgaaattgg gggatcgtct
     4501 aatggcagg
//
LOCUS       DAOOVW010000081         3958 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_12434_length_3958_cov_10.233154, whole genome shotgun
            sequence.
ACCESSION   DAOOVW010000081 DAOOVW010000000
VERSION     DAOOVW010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12434_length_3958_cov_10.233154"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="PLW95_07930"
     CDS             complement(<1..317)
                     /locus_tag="PLW95_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22583.1"
                     /translation="MKRIVESNNKEEVIVKRKDSLVLVVSFCFLFFIITISFLIITKF
                     SFINSIILSGVFLSSVYIFLFLLISILPSLKKRGGGRFYKIHPDLPLYKDRWVNLYKG
                     RSL"
     gene            complement(462..1661)
                     /gene="hisD"
                     /locus_tag="PLW95_07935"
     CDS             complement(462..1661)
                     /gene="hisD"
                     /locus_tag="PLW95_07935"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOV22584.1"
                     /translation="MKKINFSQLVEILKERRKKNEKIEDEVRNIIKRIKKEKDKGVLY
                     YTEKFDKVKITQIKVEKEEIKKCFKKIDKDVIEAIKRSSEKIEKYHKKQLPGGFILKE
                     DSYKIIFKFSPVESTGIYIPGGQSPLISTILMSTIPAKVAGVKNIHIATPPSKNIDLI
                     LGVCYFLGIENIYRVGGAQAIASFAYGTETIPEVDVIAGPGNKYVNTCKKIISGDKRI
                     DLPAGPSEVVIFSDSSGNKKFIEYDFKAQMEHTDGFGLFITTDEKIGKEISKKVVKNG
                     YYIIVKSQEEALKIINSVCPEHLQIISKTPSFLVENSIAGAIFVGDYTPAVVGDYFVG
                     PSHILPTNQSAKSFSGLSVYNFLRSYAIIEVKKNFYKKYGKYIERIAEIEGLKNHSYT
                     IKIREKY"
     gene            complement(1658..2299)
                     /gene="rpe"
                     /locus_tag="PLW95_07940"
     CDS             complement(1658..2299)
                     /gene="rpe"
                     /locus_tag="PLW95_07940"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014095495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOV22585.1"
                     /translation="MVKISPSLLSADFSCLGKEVEEITKSGADLIHFDVMDGHFVPNL
                     TFGPMILSSIRKFSNLPFQAHLMITNPEKYWRDFANSGADIIGIHIECKIPHRKLIEE
                     IKNYGKKVCLVINPPTSVDEIVPFLEIINEVLVMSVNPGFGGQKFIADVVPKIEKLNN
                     IKTQKKLDFDIEVDGGINNETAKIVKKAGVNIVVAGSYIFSSKNYKKAIESLR"
     gene            2564..3709
                     /locus_tag="PLW95_07945"
     CDS             2564..3709
                     /locus_tag="PLW95_07945"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOV22586.1"
                     /translation="MFFFFPIRDEYRVKKFPIVVISIITINCIVFFLSAFSPNYEGIV
                     LKYGFVPANPSILTAITSMFLHGGISHLGFNMWYLYLTGDNLEDRWGRFNFLFFYLLG
                     GIFSVFLYSVLIPPQHDLVPLIGASGAIAAVLGAYIVLFPKTIITFKYFCWFFLFIRV
                     GEFKLYASFWLGLWFFQQALYTFLVKKLLITSSVAFAAHFAGFIFGVIIGIGTKLFKE
                     AKYRENVELGQNLLLNIIGREKSQKVISLKEMEEIEDIKGKIKENFEKERALATSYYT
                     QLLKKYPEKATIDEKTHFEIAESLKRQGKIDIAIECYKNFLLSYPFSKLADDALFSLG
                     KIYFEKGEYEKAKNCFLQIVIFYPYSNVYEESKYYLEKQLPQYTNKD"
     gene            complement(3758..>3958)
                     /locus_tag="PLW95_07950"
     CDS             complement(3758..>3958)
                     /locus_tag="PLW95_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22587.1"
                     /translation="QSKICKALLEKKCKLIIHYADWGFDDKKQFYFLNACPILQKCIE
                     SNFELVKKYGHYWIYIAKKTNN"
BASE COUNT         1347 a          573 c          500 g         1538 t
ORIGIN      
        1 ttcaaactcc ttcccttata aaggtttacc catctatctt tgtagagggg aaggtcagga
       61 tggattttat aaaatctccc cccacccctc ttttttaaag aggggagaat tgaaataagg
      121 agaaataaaa atatatatac agaagataaa aaaactcctg ataaaataat tgaatttata
      181 aaagaaaatt ttgtgattat taaaaaagaa attgtgatta taaaaaaaag aaaacagaaa
      241 gatactacca acacgagaga gtcctttctt ttgactatta cttcttcttt attattactt
      301 tctactattc ttttcatcat ttctcttttt ttattatttt acccctttct ttccttctgt
      361 caatactcat aactttaatt tttgtaaaat gaagtaaaac acttagtata tttatatgat
      421 ttagaaaatt gtcagaaaaa cagattttaa attgaaagga attaatattt ttctcttatt
      481 tttattgtgt atgaatgatt tttcagtcct tctatttctg ctattctttc tatatatttc
      541 ccatattttt tataaaaatt ttttttaact tcaattattg cataacttct taaaaaatta
      601 taaacagaaa gtcctgaaaa tgactttgct gactgatttg ttggtaatat gtgagatgga
      661 ccaacaaaat aatcccctac tactgcggga gtataatcac caacaaaaat tgcccctgct
      721 attgaatttt caacaagaaa agagggggtt tttgatataa tttgtaagtg ttccggacat
      781 acagaattta ttatttttag tgcttcttct tgacttttta caattatata atatccattt
      841 tttacaactt tttttgaaat ttcttttcca attttttcat ctgttgttat aaaaagtcca
      901 aaaccatcag tatgttccat ctgtgcttta aagtcatatt ctataaattt tttattacca
      961 gaactatcag aaaaaataac cacttcactt ggtcctgctg gtaggtcaat ccttttatca
     1021 ccagagataa tttttttaca ggtattgaca tatttatttc caggtcctgc tataacatca
     1081 acttctggaa ttgtctcagt tccataagca aaagaagcaa ttgcctgtgc tccaccaact
     1141 ctgtaaatgt tttcaatacc aagaaaataa caaactccga gaattaaatc aatatttttt
     1201 gaaggaggag tagcaatatg aatattctta acaccagcaa cttttgcggg tattgtactc
     1261 attaaaattg ttgaaattaa aggtgactgc ccaccaggaa tataaattcc agtactttca
     1321 acagggctaa atttgaaaat tattttataa ctatcttctt ttagaataaa cccaccagga
     1381 agttgttttt tatggtattt ttctattttc tcagatgacc ttttaattgc ttcaattaca
     1441 tctttatcaa tttttttaaa acatttttta atttcttctt tttcaacttt tatctgggta
     1501 atttttactt tatcaaattt ttcagtgtaa tataaaacac ctttatcttt ttcttttttt
     1561 attcttttaa ttatatttct aacttcatct tcaatttttt catttttctt tcttctttct
     1621 tttaaaattt ctaccaattg agaaaaattt atttttttca tcttaaactt tctattgctt
     1681 ttttatagtt tttactgcta aaaatgtaag aaccagcaac aacaatatta actcctgctt
     1741 ttttaacaat ttttgctgtt tcattattta ttccaccatc aacttctatg tcaaaatcta
     1801 actttttttg ggtttttata ttatttaatt tttcaatttt cggaacaaca tcagcaatga
     1861 atttttgtcc tccaaaacca ggattgacac tcattactaa aacttcatta ataatttcaa
     1921 gaaaaggaac aatctcatca acagaagttg ggggatttat tacaagacat acttttttcc
     1981 cataattttt tatttcttca ataagttttc tatgaggtat tttgcattca atatgtattc
     2041 ctataatatc tgctccacta ttcgcaaagt ccctccagta tttttctgga tttgtaatca
     2101 ttagatgtgc ctggaaaggc aaattagaaa attttcttat tgaagataaa atcataggac
     2161 caaatgttaa atttggtaca aaatgtccat ccatgacatc aaaatgaatt aagtcagcac
     2221 cactttttgt aatttcttct acttcttttc ctaaacaact gaaatctgct gaaagcaaag
     2281 aaggtgatat tttaaccata gattgttatt ttaacatata aaagaattaa aatctaaaat
     2341 ttgaagaagt gaagaagttg acagagaata tagaaaatat aaaattaata aaggagtatt
     2401 ttgattgaag aacattaaaa tcaattttta aaactgctaa tatctctggt gaagaatttt
     2461 aaaaaactta tctaataaaa atgaattttg gagtcgctga taaaaaacaa tgatgtttcc
     2521 tttggtctat gctcagaggc atcctaaatt ataaatttaa aaaatgttct tctttttccc
     2581 aataagagat gaatatagag taaaaaagtt tccgattgta gttatttcta taataactat
     2641 taattgtatt gtttttttcc tatctgcttt ttctccaaat tatgagggaa tagttttaaa
     2701 atatggtttt gttcctgcta atccatctat tcttactgca attacttcta tgtttttaca
     2761 tggcgggatt tctcatttag gttttaatat gtggtatctt tatttaacag gagataacct
     2821 tgaagataga tggggaagat ttaatttcct atttttttat cttcttggtg gtattttttc
     2881 tgtattcctt tattcagttc ttatcccacc acaacatgac ttagttcctt taataggtgc
     2941 ttctggtgca atagcagcag ttcttggagc atatatagtt ctatttccaa aaacaattat
     3001 aacttttaaa tatttttgct ggttttttct atttataaga gttggagaat ttaaattata
     3061 tgcttcattc tggcttgggc tatggttttt tcagcaggca ctttacacat ttctggtaaa
     3121 aaaacttctt attacaagtt ctgttgcttt tgccgcccat tttgctggat ttatttttgg
     3181 agtaataatt ggaattggga caaagttatt taaggaagca aaatatagag aaaatgttga
     3241 gttggggcaa aatttacttt tgaatataat aggaagagaa aaatctcaga aagttatttc
     3301 actaaaggaa atggaagaaa tagaggatat aaaagggaaa ataaaagaaa attttgagaa
     3361 agaaagagcc cttgcaactt cttattatac tcaactttta aaaaaatacc ctgaaaaagc
     3421 aacaatagat gagaaaacac attttgaaat tgcagaatca cttaaaagac agggaaaaat
     3481 tgatattgct attgaatgtt ataagaattt tcttctttca tatcctttca gtaaattagc
     3541 agatgatgca ttattttcat taggaaaaat ttattttgaa aaaggtgaat atgaaaaagc
     3601 aaaaaattgt tttttacaaa ttgtcatttt ttatccatat agcaatgttt atgaagaaag
     3661 taagtactac cttgaaaaac aacttcctca atataccaat aaagattaaa tttcaataat
     3721 ttattttcta aataatattt aaataacatt taaaaattta attattggtt ttttttgcaa
     3781 tatatatcca gtagtgtcca tattttttaa ctaactcaaa attactctca atacatttct
     3841 gtaaaattgg gcatgcattt aaaaaataaa attgtttctt gtcatcaaat ccccaatcag
     3901 cataatgaat tattaactta cattttttct caagaagtgc cttacaaatt tttgattg
//
LOCUS       DAOOVW010000082         3700 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_13479_length_3700_cov_8.063649, whole genome shotgun sequence.
ACCESSION   DAOOVW010000082 DAOOVW010000000
VERSION     DAOOVW010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13479_length_3700_cov_8.063649"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            405..797
                     /locus_tag="PLW95_07955"
     CDS             405..797
                     /locus_tag="PLW95_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22588.1"
                     /translation="MTSPPGENLDLKWTGWEIDPERKTTYLREDEWLTQPNNIKNHFS
                     RVSPSSGYILKSDGWEIMACLKYKDSSEVPYLLLHKVGNGFLVITSAEIGYGGGWYTF
                     GTPYYKEAGMLIENLYNMFLKDLSMVIF"
     gene            1283..3373
                     /locus_tag="PLW95_07960"
     CDS             1283..3373
                     /locus_tag="PLW95_07960"
                     /inference="COORDINATES: protein motif:HMM:NF024597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HOV22589.1"
                     /translation="MKKYSIPKLQHKGHLLKVFTEYWEVEQDINRGGSIVSIVFVNGS
                     GKNILRKPLLSYVDKMGDVLSFMSAGKRPEHSEEKLFFSTSSEKKPKVEIDKTSAGFP
                     IIISSGFLYNNKGETTGISYSEQCEYHSSFIRRTITFQFDEPANDILVLGVAKIVIDR
                     SLCEYAYKYSAWQARHGGNLLTNLIYGKVYPFAYPVYVERYPFRYLSLFNRGKDAIEF
                     LPSSNVELWETGITGTPGSGFFGLYTLEDKRGNTILMEPFHELTLGYPDPKYKRPELK
                     GKYSFTFTIGLPMIEKNYPADIRHVGFNNHPWPDEKEIRKWAEYGVNVARLHNDFDKS
                     GNFWHDGTFPPYDEEDIKKMKETIATAHRYGIKVIPYFSLSELHPSSEAWKKNHDFWK
                     RTEDDNDTEIHNYWQNGEYGAQMCLASGWNDYLKNYIKKIVETYGFDGVYFDWVSSLY
                     CNNRRHKEYNHLTCDEILEFLEWTRNFLGTNRVLVIHTSASPFMAAESYADATIVFEE
                     IYAPPAFMKDMPPIDCLQPQLDMANHIQKLVCPSILAFKGKTAAKKFVSSCIAMGLLS
                     YIGRQKGSSDPFIENFQRLRKYKLTNYAVIPATRAPVVVNKKNIRVCFFVNNSEIIVI
                     ITNLSAKKELFNIQFNPSAVNPIWNKYKRYLIEDKDGKRKKTVGLNGIKGSGQISGYD
                     YIIWRIIPILKRKT"
BASE COUNT         1325 a          531 c          644 g         1200 t
ORIGIN      
        1 tttgtaatga ttaaaaaaga aattgtgatt ataaggaaaa aacaaaaaga tactacaaat
       61 atgagaaatc cgcttctttt aactattact tcttctcttt tattactacc tattattctt
      121 tttatcattt ttctttcttt aattatacta tccttttttc cctctgtcaa aacattgtat
      181 tgagacataa taaatctaca cgaaagggta tgttagacaa aacaaaaaaa atctacttga
      241 aaaataggat ttaaatagca tctccggata aaataagaaa aaactggagg tgcaaatgaa
      301 aaaggaatta gaaatgggga taaggtgtca ataagcatct taaatgcacc actaatcgtc
      361 agttgatact gcaccacttt tttaataata ccactgtgta aattttgaca tcacctcctg
      421 gtgaaaatct tgatttgaaa tggacaggat gggaaataga ccctgaacga aaaacaacat
      481 atcttcgtga agatgaatgg cttactcaac ctaataatat caaaaatcat ttttctagag
      541 tgtctccttc ttctggttat attcttaaat cagatggatg ggaaataatg gcttgtttaa
      601 aatataaaga tagttctgaa gttccatatt tgttattgca taaagttggt aatggatttt
      661 tagtaattac atctgctgaa ataggatatg gtggtggctg gtataccttt ggaacacctt
      721 attataaaga agcggggatg ttaattgaaa atctttacaa tatgttttta aaagatttat
      781 ctatggttat attttgaaaa gggtaaatct taattgaact ccataagagc agtcctaatt
      841 tttatcatct aagatatctt attgctcaat agatatatct aactaagctt gaaaaagtca
      901 ttttaagtca gtttcagaat taagagagtt tttattttct gagcaagttt atttgataag
      961 gttcttagag tttaagtttt tttcctttat gtttgtcaat atctcttttt gtttttttaa
     1021 aaatatatca aaacccacat atattaagga catgcagaac acttgacaaa aacttggggg
     1081 gataatgaaa acagaattaa aacttgtcaa ttgatagtaa atatgttaag gtttaattga
     1141 taaaaaaatt tgaactttaa cttgctcact ctgcttttaa tagagtacaa aagtagataa
     1201 atattcttat cagaaagatg gggttgagta aatacttaca aaaaagagtg atgtataata
     1261 aaagcagaaa ggagaaattt ttatgaagaa atattctatt cctaaattac aacataaggg
     1321 acatctatta aaggttttca cagaatattg ggaagtagag caggatataa atcggggagg
     1381 ttctattgta tctatagttt ttgttaatgg gtccgggaaa aatattcttc gcaagccctt
     1441 actctcatat gtggataaaa tgggagatgt tctttctttt atgtctgcgg gaaagagacc
     1501 agaacacagc gaagaaaaat tatttttcag cacctcttct gaaaaaaaac caaaggtaga
     1561 aattgataaa acaagtgctg gttttcctat aattatctct tcaggttttt tgtataacaa
     1621 taaaggagaa acaactggta tttcttatag cgaacaatgt gagtatcatt caagttttat
     1681 ccgaagaaca attacctttc aatttgatga acccgcaaat gacattttag ttctcggagt
     1741 tgcaaaaatt gttattgata ggtctctatg tgaatatgct tataaatatt cagcatggca
     1801 ggcaagacat gggggtaacc ttttgaccaa tcttatctat ggtaaagttt atccctttgc
     1861 ctaccctgta tatgttgaac gatatccttt cagatattta tccctcttta atagaggaaa
     1921 agatgctata gaatttttac cttcgagtaa tgtggaatta tgggaaactg gcattaccgg
     1981 tacccctggt agtggctttt ttggtttata tacacttgaa gataaaaggg gaaatactat
     2041 attgatggaa ccttttcatg aacttacctt agggtaccct gaccctaaat ataaaagacc
     2101 tgaactaaaa ggtaagtatt ccttcacttt tacaatagga ttaccaatga ttgagaaaaa
     2161 ttatcctgcg gatatcagac atgtgggatt caacaaccac ccctggccgg atgaaaaaga
     2221 aattcgtaaa tgggctgaat atggtgttaa tgtagcaaga ttgcataatg attttgacaa
     2281 aagcggtaat ttctggcatg atgggacatt tcctccctat gatgaagagg atattaaaaa
     2341 aatgaaagaa acaattgcaa ctgcacatcg ttatggaata aaagtgattc cttatttttc
     2401 tcttagtgaa ttacatccat cttcagaagc atggaaaaaa aaccatgatt tttggaagcg
     2461 cacagaagat gacaatgata cagaaataca taattattgg caaaatggag aatatggagc
     2521 acaaatgtgt cttgcttcgg gatggaatga ctacttaaaa aattacatta aaaaaattgt
     2581 agaaacctat ggatttgatg gtgtctattt tgattgggtt agttccttat actgcaataa
     2641 tagaagacat aaggagtata accatcttac ttgcgatgaa atccttgaat ttctggaatg
     2701 gacaagaaat tttctgggaa caaacagggt attagtaatt cacacttctg catctccttt
     2761 tatggcggca gaaagttatg ccgatgctac tattgttttt gaagagattt atgcgccccc
     2821 tgcatttatg aaggacatgc cacctattga ttgtttacag ccacagttag atatggcaaa
     2881 tcacatacaa aaacttgtct gtcccagtat acttgctttt aaggggaaaa cagcagccaa
     2941 aaaatttgtt tcaagttgca ttgcaatggg acttctttca tatattggaa ggcagaaagg
     3001 aagtagtgac ccttttatcg agaacttcca gaggctgcgt aaatataagt taactaacta
     3061 tgctgtaata cctgctacac gtgctcctgt tgtcgttaat aaaaaaaata taagggtttg
     3121 cttttttgtc aataacagcg aaattattgt tataataacc aaccttagtg ctaaaaagga
     3181 attattcaat atacaattca atccatcagc agttaatcct atatggaata agtataaaag
     3241 atacttaatt gaggataaag atggaaagag aaaaaaaact gttggattga acggaataaa
     3301 aggaagtgga caaatcagtg gatatgacta cataatatgg cgtattatcc ctattttgaa
     3361 gcgaaaaact taataaaaag ataaaacgtt cgttatgtta ggcacaaaaa cacaacaaag
     3421 ggaacaaaga agaatgctaa atttaggcaa agaggaaaaa caaaatataa atagatagaa
     3481 tattttactt tgttcctttg tatgagcaca tctttcactg atatatttac ttatatgaag
     3541 aattattaaa acactatttt cgtatatatt ttttatgagg caaagtgttg acaaatcaac
     3601 ttcgccattt aacaatttct gacatttaac aaatccttat tatcctaacc taaaatcccg
     3661 ttttttataa ctatgtatta tattcaggta taggtaaatc
//
LOCUS       DAOOVW010000083         3667 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_13636_length_3667_cov_6.838317, whole genome shotgun sequence.
ACCESSION   DAOOVW010000083 DAOOVW010000000
VERSION     DAOOVW010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13636_length_3667_cov_6.838317"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="PLW95_07965"
     CDS             <1..1059
                     /locus_tag="PLW95_07965"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HOV22590.1"
                     /translation="PIKTFSIGFDVKEYNELKYAKIVAEKFGTQHHEFIIQPDIIKCL
                     PKLVWHYNEPFADSSMIPTYYLAKETSNYVKVALNGDGGDENFAGYDRYYQTMVLSKL
                     YSFCKKAGLTSSYVKNLIEKIYQKFPAPFPVRILEWIKEADDFGFGFAYSRRLQSFSD
                     IWKEKIYSPWFKNELKNYNSIEIVDKLWEKNKADNLLEKMLYTDFHLYLPEVLMVKID
                     IATMANSLEGRSPFLDYKFIETISSFPPELKLKGKISKYILKKKLKGFLPDEILKRGK
                     MGFGVPVGEWFKGELANYLKSIILNKRFFKRNFFEKKYVEEIVQQHISGRVNHSSRLW
                     TLLCFEIWCRIFLDGEQV"
     gene            1056..2285
                     /locus_tag="PLW95_07970"
     CDS             1056..2285
                     /locus_tag="PLW95_07970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOV22591.1"
                     /translation="MKKEFNSNNYTKGIWKKLLFIFICSLLIRVLFILTLNNSIDVWG
                     DWWDELGWKLASGKGFWVENPYFPGGQKFYSWRTPGFPFFLAIIYKIFGHNYLAAKIG
                     LAIISSFTCILIYFLLREFFNSKISFIGSFLYSIYPSSIFWTGYLAPETLETFLLVLF
                     SIFLIKGKNKKSDTFILLSGIFLGIAVLTRSLFFIFLPLIFFYFLIEFKRKGILKFIF
                     LFLGCFLVITPWVLRNYKIHKKLVLTSTEGGIVCYIANNEKSLSQPSGYWNPPPPFFE
                     KFNGMSEVEIDHKLYELALNFILSHPKEYLKLVWDRFIRYWRFFPHTFSGPGENYKKY
                     HVILGFITFAPIIILSFIGFFITLKNLKKYLWIYFGILGWSLPTILLFKTVIRYREPI
                     IPYLMVLLLSIFKNVEK"
     gene            2272..2991
                     /locus_tag="PLW95_07975"
     CDS             2272..2991
                     /locus_tag="PLW95_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009761128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOV22592.1"
                     /translation="MWKNKKVSVIFPTYNERDSIREAIDDFFASSFVDEIVVVNNNAV
                     EGTDEEVKKTKAKLVYEKRQGYGYAIWKGLEEATGDYLIISEPDGTFLGRDVIKLLAY
                     SDDFDYVLGTRTTRELIWKGANMNFFLKWGNWAVAKIMEFLYNTTILTDVGCTMRLIK
                     RNIYIKVKPYFTVGEQHFGPELTLLVIKSGTKFVEIPVNYKPRVGKSSVTGNMGKAFS
                     LGLKMIILIFKYKFKRINFEK"
     gene            2988..>3667
                     /locus_tag="PLW95_07980"
     CDS             2988..>3667
                     /locus_tag="PLW95_07980"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOV22593.1"
                     /translation="MILCYHRINPWYKKDAITVSPTKFHKQINYLFSKNFKFLIMDKY
                     IEDFFISNDKKVVISFDDGYADNLWFAIEILNDFGISPIIYLTVSYIGTNILFPRYKE
                     ILKDRFLNWKEVSEMVNNGVEFGSHTLTHPDLTKIDKKQAEKEIIESKKIIEDKIGKE
                     TKFFCYPYGNFNSEIIEIVQKAGYKGAVVNRKKNMEITKFTLPRVGIYSHNNNFIYRV
                     KIWREYIK"
BASE COUNT         1340 a          422 c          586 g         1319 t
ORIGIN      
        1 cctataaaga cattttcaat tggttttgat gtgaaagaat ataatgaatt aaaatacgct
       61 aaaattgttg cagaaaaatt tggaacacaa caccatgaat ttattatcca accagatatt
      121 atcaaatgtt taccaaaact tgtctggcat tataatgaac catttgctga ttcttctatg
      181 attccaactt attatttggc aaaagaaact tcaaattatg taaaagttgc cttaaatgga
      241 gatggtggag acgaaaactt tgctggatac gatagatatt atcaaacaat ggtattatca
      301 aaactttaca gtttttgtaa aaaagcagga ctcacttctt catatgtcaa aaatttgatt
      361 gagaaaatat atcaaaaatt tcctgctcct ttccctgtaa gaattttaga atggattaaa
      421 gaggcagatg atttcggttt tggtttcgca tattcaagaa gattacaatc tttttcagat
      481 atatggaaag aaaaaatata ttctccttgg tttaaaaatg aacttaaaaa ttataactca
      541 attgaaatag ttgataaatt atgggaaaaa aataaagcag ataatttatt agaaaaaatg
      601 ctttatactg actttcatct ttatttacca gaagttctaa tggtcaaaat agatatagca
      661 acaatggcaa attctcttga aggaagaagt ccttttcttg attataaatt tattgaaaca
      721 atatcttcat ttccaccaga attaaaatta aaagggaaaa tttctaagta tattttgaaa
      781 aaaaaattaa aaggattttt gcccgacgag attttaaaaa gggggaaaat gggctttggt
      841 gtcccagttg gtgagtggtt taagggagaa cttgcaaatt atttaaaaag cataatttta
      901 aataaaagat ttttcaaaag gaactttttt gaaaaaaaat atgtagaaga aattgttcag
      961 caacacattt ctggtagagt taatcattct tctcgtttat ggactttatt atgttttgag
     1021 atttggtgta gaatatttct tgatggagaa caggtatgaa aaaagagttt aactctaaca
     1081 attatacaaa aggaatttgg aaaaaattat tatttatatt tatttgttct cttttaataa
     1141 gagtactgtt tattcttact ttaaataatt caattgatgt ttggggtgat tggtgggatg
     1201 agttgggatg gaaacttgcc tcaggaaaag gattctgggt agaaaatcct tattttccag
     1261 gtggacagaa attctattca tggagaactc cgggatttcc tttttttctt gcaataatat
     1321 ataaaatttt tggtcataat taccttgctg caaaaattgg gcttgctata attagttcat
     1381 ttacttgcat tttgatttat ttccttctta gagagttttt taattcaaaa atttctttta
     1441 taggcagttt tctttattca atatacccat cttcaatttt ctggactggt tatcttgcac
     1501 ctgaaacact tgaaaccttt ttattagttt tattttcaat attcttaata aaaggcaaaa
     1561 ataaaaaaag tgatactttt attttattaa gtggtatttt tcttggaatt gccgttttaa
     1621 cgaggtctct tttttttatt tttcttcctt taatattttt ttattttctt atagaattca
     1681 agagaaaagg gatattaaaa tttatttttt tgtttttagg ctgttttctt gtaataactc
     1741 cttgggttct aaggaattac aaaattcata aaaaacttgt tcttacttct actgagggag
     1801 gaattgtttg ttatattgct aataatgaaa aatcactttc tcaaccatca ggatattgga
     1861 atcctcctcc tccttttttt gaaaaattca atgggatgtc agaagttgaa attgaccata
     1921 aattatatga acttgcttta aattttattt tatcccatcc aaaagaatat ctaaaacttg
     1981 tctgggatag atttataaga tattggagat ttttccctca tactttttct ggtcctggtg
     2041 aaaactataa aaaataccat gtaatacttg gatttataac ttttgcccct attataattc
     2101 taagttttat aggtttcttt attactctta aaaatttgaa aaaataccta tggatttatt
     2161 ttggaattct tggttggtcg ttaccgacaa tccttttatt caaaactgta ataagatata
     2221 gggaaccaat aattccatat ttgatggttt tattactttc aattttcaaa aatgtggaaa
     2281 aataaaaagg tctcagtaat atttccaaca tataatgaaa gggactcaat aagagaagca
     2341 atagatgatt tttttgcgag ttcttttgtt gatgaaatag ttgttgttaa taataatgcc
     2401 gttgaaggaa ctgatgaaga agtgaaaaaa acaaaggcaa aacttgttta tgaaaaaaga
     2461 cagggatatg gatatgcaat ttggaaagga cttgaagaag caacagggga ttatttgata
     2521 atttcagaac cagatggaac ttttcttgga agagatgtaa ttaaactact tgcttatagt
     2581 gatgattttg attatgtact tggaacaaga acaacaagag aattaatatg gaaaggtgca
     2641 aatatgaatt tttttctgaa gtggggaaac tgggcagtgg caaaaataat ggaattttta
     2701 tataacacta caattttaac agatgttgga tgtacaatgc ggcttataaa gagaaatata
     2761 tatataaaag ttaaaccata ttttacagtt ggagaacaac attttggtcc agaacttacc
     2821 cttcttgtta taaaatcagg aactaaattt gttgaaatac ctgtaaatta taaaccaaga
     2881 gttggaaaat catcagtaac tggaaatatg ggaaaagcat tttctcttgg attaaagatg
     2941 attattttaa tttttaagta taaattcaaa aggataaatt ttgaaaaatg atactatgct
     3001 atcacagaat taatccatgg tataagaaag atgcaataac agtcagtcct acaaaattcc
     3061 ataagcagat taattattta ttttctaaaa attttaaatt tcttataatg gataaatata
     3121 tagaagattt ttttatttct aatgataaaa aagtcgtaat ttcatttgat gatggatatg
     3181 ctgataattt atggtttgct attgaaatac ttaatgactt tgggatttct ccaataattt
     3241 atcttactgt ttcttatatt ggaactaata tccttttccc aagatataaa gaaatattga
     3301 aagatagatt tcttaattgg aaagaagtat cagaaatggt aaataatgga gttgaatttg
     3361 gttcacatac actaactcat cctgatttaa cgaaaattga taaaaagcaa gcggaaaaag
     3421 aaataattga atcaaagaaa attattgaag ataaaatcgg taaagaaaca aaattttttt
     3481 gttatccata tggcaacttt aattctgaaa taattgaaat tgtacagaaa gcagggtata
     3541 aaggggctgt tgttaataga aaaaaaaata tggaaataac aaaatttaca ttacccaggg
     3601 ttggtattta ttctcataat aataatttta tttatagggt caaaatatgg agagaatata
     3661 taaaaga
//
LOCUS       DAOOVW010000084         3660 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_13673_length_3660_cov_8.281831, whole genome shotgun sequence.
ACCESSION   DAOOVW010000084 DAOOVW010000000
VERSION     DAOOVW010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13673_length_3660_cov_8.281831"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2574)
                     /locus_tag="PLW95_07985"
     CDS             complement(<1..2574)
                     /locus_tag="PLW95_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22594.1"
                     /translation="MSIKITEIAVKNCGPISEFKESFGSINLIYSKNERGKSFLVEFI
                     IRCLFKNKNNWGYLRESGEGKVTIQGLDEKNIDFRPNRGVKLEDYLEKKQGLPLSLVK
                     LMVVKEGETDIVDKNESGIDKNTIKNILSPRKLLDSILNKISKTVRKGEIINLGIDIA
                     NQGDGETYNKKREEFYRLNEIVDKLTKDYEQGIIHNITIQIEKLKEEKEILIKSKKYK
                     GYKLSREIHNLEEELEKIPEFSIKELKELITEYKNKRNQIEDINREITRIRKQTEKLP
                     DLEEKHKKLLQAKKYQGYLISEELKKREGEINKFPEEEIKKIEQGISNYQNKKNELKE
                     KEKQINELKENSKGYEWLKAAKEEYTKISNTPVIPKQPLFLYITFILFLITGLVLISL
                     NYRIYGIILIVLGTISEICYLHKIKKDIVSYKQADELISLKNDFKSRFGTSLNNLVQI
                     ETLLNEQQKAHNCLDILEGEQQRLKIEVNTLFNSIIDGFKRLKIESPLDETNWNNILS
                     DIYTKRKKLEEECQKLQKKLDGLDIDESQYQIENPGIEFDKDEMEKIEKEIEKIKTLK
                     EEEERKSGQYEDLKNQLSKNKEKINALFQNITGETIDEGYWEEKINEILDKRERHNNE
                     INKKIGELKGLGVGENEYVEEDPGKEYSEEELKKVEKAIGEMENKRSEEEKKLNDLKY
                     TICQYTGSDISCDWSVLIDNLFKKREEIKNAFQEIEAKILSGIIVNKTIEKLQEEEDK
                     ELLESLNSEEVKNFLFKLTGRYKQLLFNDSDIIISDDYNEFSLKDLSTGAKEQVMIAL
                     RIGFLSHILKTDSAFLILDDAFQHSDYDKREILIKSLFELAMNNWQIIYLTMDDHIKD
                     LF"
     gene            complement(2571..>3660)
                     /locus_tag="PLW95_07990"
     CDS             complement(2571..>3660)
                     /locus_tag="PLW95_07990"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOV22595.1"
                     /translation="MLYIIDMKIAITADVHLKEQNETPDRYRVVESIFNECISKQITT
                     LLIAGDTFDKNFFQYQSFDKICGKFKNIKVIIIPGNHDTEVKQEFFTSGNIEVINSVS
                     LKTFDGISFLLIPYISTKTMDEVLIEFISENKPDNWILVGHGDYITTNWQTNPYEPGT
                     YMPLSAAVINKYNPLKVFLGHIHKADNFGKVYYPGSPFPLDITETGKRQFIIYDTGAN
                     TIENQFIKTEKIYFIEEILTFPVENEIDLIKKQIEQMIKNWKLPDEELNYVNLRLKVR
                     GFTTNLLSLKGAIINSLSKYGISLYDEEGPDLTDIKVLNTLSDERIFIFNKVKQEIEK
                     LELKNFHATKEQILEKAMELIFGELDKK"
BASE COUNT         1062 a          600 c          381 g         1617 t
ORIGIN      
        1 aaataaatct ttaatgtggt catccattgt aagataaatt atctgccagt tattcattgc
       61 taattcaaaa agggatttta ttaaaatttc tcttttatca taatcagaat gctggaatgc
      121 atcatcaagt attaaaaaag cactatctgt ttttaatata tgacttaaaa aaccaattct
      181 taaagcaatc ataacctgtt cttttgcccc tgtgcttaaa tcttttaaag aaaattcatt
      241 ataatcatct gaaataatta tgtctgaatc attaaacaat agttgtttat atctgcccgt
      301 taatttaaaa agaaaatttt taacttcttc ggaattaagt gattctaata gttctttatc
      361 ttcttcttcc tgtaattttt caattgtttt atttacaata attcctgata aaatttttgc
      421 ttcaatttcc tggaaggcat tttttatttc ttctctcttt ttaaataaat tatcaattaa
      481 aacagaccaa tcacaactta tatcactgcc agtatactga catatagtat attttaaatc
      541 atttaatttc ttttcttcct cactcctttt gttttccatt tcacctattg ccttttcaac
      601 tttttttaat tcttcttctg aatattcttt accagggtct tcctctacat attcattttc
      661 tccaacaccc agccccttta actcaccaat ttttttattt atttcgttat tatgtctttc
      721 tcttttgtcc aatatttcat ttattttttc ttcccagtac ccttcatcaa ttgtttctcc
      781 tgttatattc tggaaaaggg cattaatttt ttctttattt tttgataatt gattttttaa
      841 atcctcatat tgcccacttt ttctttcttc ctcttctttt agtgttttta ttttttctat
      901 ttctttttct attttttcca tctcatcttt atcaaactct attcctgggt tttctatttg
      961 atactgactt tcatcaatat ctaatccatc taattttttt tgtaattttt gacactcctc
     1021 ttctaatttt tttctttttg tataaatatc agaaaggata ttattccagt tggtttcatc
     1081 cagtggactt tcaattttta gtcgtttaaa cccatcaata atggaattaa aaagagtatt
     1141 tacttcaatt tttaatcgct gttgttcgcc ttcaagtata tcaagacagt tatgtgcctt
     1201 ttgttgttcg ttaagtaatg tctcaatttg aaccagatta ttaagagaag ttccaaaacg
     1261 acttttaaaa tcatttttta aagaaataag ctcatctgcc tgtttataag aaacaatatc
     1321 tttttttatt ttatgtaaat aacatatttc tgatattgtc cctaaaacaa tcaatataat
     1381 tccatagatt ctataattta aagaaataag aactaatcca gttattaaaa atagaataaa
     1441 agtgatatat aaaaataatg gctgtttagg aataacagga gtatttgaaa tttttgtata
     1501 ttcttctttt gctgctttta accattcata accttttgag ttttctttta attcatttat
     1561 ctgtttttct ttttctttca attcattttt tttattttga taatttgaaa ttccctgttc
     1621 tattttttta atttcctctt ctggaaattt atttatttca ccttctctct tttttaattc
     1681 ctctgaaata agataaccct gatatttttt tgcttgaaga agttttttat gtttttcttc
     1741 taagtcaggt aatttttcag tttgttttct tatccttgtt atttctctgt ttatatcttc
     1801 aatctggttt cttttatttt tatattcagt tatcaattcc ttcaattcct ttattgagaa
     1861 ttcagggatt ttctcaagtt cttcttctaa attatgtatt tcccttgata gtttatatcc
     1921 cttatatttt ttactcttaa tgagtatttc cttttcctct tttaattttt caatttggat
     1981 tgttatgtta tgtataatcc cctgttcata atcttttgta agtttatcaa ctatttcatt
     2041 taatctataa aattcttctc tttttttgtt ataagtttct ccatcaccct ggtttgcaat
     2101 atcaattccc aaatttatta tttcaccttt tctaactgtt ttagaaattt tatttagaat
     2161 tgaatccaat aatttacggg gagacaaaat attttttatt gtatttttat caattccgct
     2221 ttcattttta tcaacaatat ctgtttcacc ttctttaaca accatcagtt ttacaagaga
     2281 taatggtaat ccctgttttt tttctaaata gtcctccaat tttactcctc tatttggacg
     2341 aaaatctata tttttttcat ccaatccttg aatagtaact ttcccttctc ctgattctcg
     2401 tagatacccc caattatttt tatttttgaa tagacatcta attatgaatt ctacaagaaa
     2461 tgattttcct ctttcatttt tgctgtaaat aagatttata cttccaaacg actctttgaa
     2521 ttcactgatt ggaccacaat ttttaactgc tatttcagtt atttttatac tcatttttta
     2581 tccaattcac caaatattaa ttccatcgct ttttctaaaa tttgttcttt tgttgcatga
     2641 aaatttttta attctaattt ttcaatctcc tgttttactt tattaaatat aaaaattctt
     2701 tcatcggaga gtgtatttag aactttaata tctgtcaggt cgggtccctc ttcgtcatat
     2761 aaggaaattc catattttga aagagaattt ataattgccc cttttaaaga taacaggttg
     2821 gtggtaaaac ctctcacttt aagacgcaag ttgacataat ttaattcctc atcgggtaat
     2881 ttccagtttt ttatcatctg ctctatctgt ttttttatta agtcaatttc attttcaaca
     2941 ggaaatgtta aaatttcttc tataaagtaa attttttctg ttttaataaa ttgattttca
     3001 atagtatttg cccctgtgtc ataaataata aattgccttt tgcctgtttc agttatatca
     3061 agaggaaatg gagaaccggg ataatatact ttcccaaaat tgtctgcttt gtgtatatgc
     3121 ccgagaaaaa cttttaatgg attgtatttg ttaattactg ccgcagaaag aggcatataa
     3181 gtccctggct cataagggtt tgtctgccag tttgtagtaa tataatcacc gtgtccaacc
     3241 aaaatccaat tatctggttt attttcagaa ataaattcaa ttaaaacttc atccattgtt
     3301 ttagttgaaa tataaggtat taagagaaaa gatattccat caaatgtttt taaagataca
     3361 gaatttataa cttctatatt acctgatgta aaaaattcct gcttaacctc tgtatcatga
     3421 ttaccaggaa taattattac tttaatgttc ttgaatttcc cacaaatttt atcaaaactc
     3481 tgatattgga aaaaattttt atcaaatgta tcacctgcaa ttaaaagagt ggtaatttgt
     3541 tttgaaatac attcattgaa gatactttca acaactctgt atctatctgg tgtttcattt
     3601 tgttctttta aatgaacatc agcagtaatg gcaattttca tatctataat gtaaagcatt
//
LOCUS       DAOOVW010000085         3659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_13677_length_3659_cov_6.413152, whole genome shotgun sequence.
ACCESSION   DAOOVW010000085 DAOOVW010000000
VERSION     DAOOVW010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13677_length_3659_cov_6.413152"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1235
                     /locus_tag="PLW95_07995"
     CDS             <1..1235
                     /locus_tag="PLW95_07995"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOV22596.1"
                     /translation="QNKKTISVGGDNRLTTPEIKEKLIKGLLSTGCEVFDIGIVPTPV
                     LYFSVHYYCFDSGIMVTASHNPPQYNGFKIVIGNRSIYGKEIQEIADIIEREDFKNDK
                     GKVSKEIADVDYINYIKSKFNFKNKFRVGVDTGNGIAGPIIVPLFKELGIKIFPLYIE
                     SDGNFPHHLPDPVVPENLKDLIKIVKEYSLDVGFGFDGDGDRLGVVDDTGEIQWGDRL
                     LILYSREILKKMPKSKIIFDVKCTKALQEEIEKAGGIPIMWKTGHSLIEDKLHQENSP
                     LAGELSGHLYFADEYYGYDDAIYASLRLLKILDNEGKKPSQLFSDVKKYFSTPEIRIE
                     VPDEKKFDIVEKLKNYYKGKYPSSEIDGIKVYFPDGWALVRASNTQPALVIRIESESQ
                     EALNKIKNEFLEVIEKFK"
     gene            1505..1975
                     /locus_tag="PLW95_08000"
     CDS             1505..1975
                     /locus_tag="PLW95_08000"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOV22597.1"
                     /translation="MKKILILFLGFLFISCIAEEQQKKAPVFSLKTVNNEIINLSDYK
                     GKVVIVNFFATWCPPCKKEIPDFVKFYNENKDKGIVVIGICVGSKEQDIKKLIEEYKI
                     SYPVCISDGKVENAYGGINAVPTTFIIDRNGFIFTKQIGMMEEEQLYNILKQIK"
     gene            1972..3645
                     /locus_tag="PLW95_08005"
     CDS             1972..3645
                     /locus_tag="PLW95_08005"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOV22598.1"
                     /translation="MKLEEIIEKLKGKGLREEQIKIVLEEKRISNSPFGYLLIKFGLV
                     SADDWYNFVLKELKIVPIKIENMDISKEVLNILPEFLCRKYRTIPIFKGGNKIVCAMV
                     DPVDESVIEELKKITGKEIEPRLTRDEEIRNALEKYLTQGGLDVILPVSKSQTIGEVY
                     QPVGIEQIRDDSAVAAFEDIISKASELKATDIHLEIEEEGIRLRYRINGLLYEFPPPP
                     LELYTPIVSHIKVLSNLDIAEKRIPQDGYCKLKLKDRDIDLRVSTFPTIFGEMIAIRV
                     LDKKNIISGLEQLGFLPETLHRWRMLLEEPYGIILVTGPTGSGKTTTLYSSLNELDNT
                     HKKIMTVEDPVEYHLKNVDQTQINPRAGLTFAVALRSILRQDPDVVMVGEIRDLETAE
                     IAFRAAQTGQLVFSTLHTNTAPGAIIRLLDMGVEPYLISSSLIAILNQRLVRSICSGC
                     KSEYKPIDEEIKTLDPTLLGKDIKYYTGRGCNLCNGTGFGGRIGIFELMIMNEELRRL
                     TMRKSDLRDIMEEARKTGMRILREDGVIKVIEGITTINEVIYSTRKDSE"
BASE COUNT         1419 a          421 c          696 g         1123 t
ORIGIN      
        1 aacaaaataa aaaaacaatt tcagttggag gagataatcg cctaacaact ccagaaataa
       61 aagaaaaatt aataaaaggg cttctttcaa ctggctgtga agtttttgat ataggaatag
      121 ttcctactcc tgtactttat ttttccgttc actattattg ttttgattca ggaattatgg
      181 ttactgcaag tcataatcct cctcaatata atggatttaa aatagttatt ggaaacaggt
      241 ctatttatgg aaaggaaatt caggaaatag cagatattat tgaaagagaa gattttaaga
      301 acgataaagg taaggttagt aaagaaattg ctgatgttga ttatataaac tatataaaaa
      361 gtaaattcaa ttttaaaaac aaattcagag taggagttga tacaggaaac ggtattgcag
      421 gtcctataat agttccctta tttaaagaac ttggaattaa aatttttcct ttatatatag
      481 agagtgatgg taattttcct catcatcttc cagaccctgt tgttccagaa aatttaaaag
      541 accttataaa aattgtcaaa gagtactcat tagatgttgg ttttggtttt gatggagatg
      601 gtgaccgact tggagttgtt gatgatacag gggagataca atggggggat agattgctga
      661 ttttatattc aagagaaatt ttaaagaaaa tgccaaaaag taaaataata tttgatgtta
      721 aatgtacaaa agcacttcag gaagaaattg aaaaagcagg tggtatacca ataatgtgga
      781 aaacaggtca ttcattaata gaagataaat tacatcagga aaattctcca cttgctggag
      841 aattatcagg gcatctttat tttgcagatg aatattatgg atatgatgat gctatttatg
      901 cgagtttaag attattaaag attcttgata atgaaggcaa aaaaccttct caactttttt
      961 cagatgttaa aaaatatttt tcaacgccag aaataagaat agaagtaccc gatgaaaaaa
     1021 aatttgatat tgttgaaaaa cttaaaaatt attataaagg aaagtaccct tcaagcgaaa
     1081 ttgatggaat aaaagtttat ttcccggacg ggtgggcact tgtcagggca tctaatacgc
     1141 aaccagcact tgttatcagg attgaaagtg aatcccaaga ggcattaaat aaaattaaaa
     1201 atgaattttt agaagttatt gagaagttta agtaaatttt tatgtgggtt aacaatattt
     1261 gtaaatcaat aattcacaaa tataaaaggg ggtgagggaa aatgaaatgc cctctaaggt
     1321 tgcggatgca aaaaagacca ctgggagaat ctgcttttgg tgttcctgaa caaattgagg
     1381 attttgattt ttgtcttgaa aaagaatgtg catgatggga tgaggaaaat aattcctgta
     1441 ttttatatca tctaatagaa atagatgaaa agttaactat tttaattgaa aggaggtaag
     1501 aaaaatgaaa aaaattttga ttttattttt gggattttta tttatttctt gtattgcaga
     1561 agagcaacaa aaaaaagcac ccgttttttc tcttaaaaca gtcaataatg aaataataaa
     1621 tctttctgac tataaaggga aagttgtaat tgttaatttt tttgccacat ggtgtcctcc
     1681 ctgtaagaaa gaaatccctg attttgtaaa attctataat gaaaataaag ataaaggaat
     1741 tgtagtaatt gggatatgtg ttgggagtaa agaacaggat ataaaaaaac ttatagaaga
     1801 atataaaata agttatcctg tttgtataag tgatggaaag gttgaaaatg cctatggcgg
     1861 aataaatgct gttcctacaa cttttataat tgatagaaat ggttttatat ttacaaaaca
     1921 aataggaatg atggaagaag aacaactata taatatttta aaacaaataa aatgaaatta
     1981 gaagaaatta ttgagaaatt aaaaggtaaa ggactgagag aagaacaaat aaaaattgtt
     2041 ttagaagaga agagaataag caattctcct tttggttatc ttttaataaa atttggtttg
     2101 gtaagtgctg atgattggta taattttgtt ttaaaggaat taaaaatagt acctataaaa
     2161 attgaaaata tggatatatc caaggaagtt ttgaatatat taccagaatt tttatgtaga
     2221 aaatacagaa caataccaat tttcaaaggc gggaataaaa ttgtttgtgc aatggttgac
     2281 cctgtagacg aaagtgtaat tgaagaactt aaaaaaataa caggaaaaga aattgagcca
     2341 cgccttacaa gagatgaaga aataagaaat gcacttgaaa aatatcttac acagggaggt
     2401 cttgatgtaa ttttgcctgt aagtaaatca cagacaattg gtgaagttta tcaaccagtt
     2461 ggaattgaac aaataaggga tgattcagca gttgctgctt ttgaagatat aattagcaag
     2521 gcaagtgaac ttaaagcaac agatattcat cttgaaattg aggaagaagg aattaggtta
     2581 agatatagaa taaatggcct tttatatgaa tttccacctc cacctcttga attatacact
     2641 ccaattgtct ctcatataaa agttctttca aaccttgata ttgcagagaa aagaattcca
     2701 caggatggat attgtaaatt gaagttaaaa gatagggata tagacctgag agtctcaaca
     2761 tttcccacta tatttggaga gatgattgca ataagagttc ttgataaaaa aaatataatt
     2821 tctggacttg aacaattagg atttttacca gaaacattac atagatggag aatgctactt
     2881 gaagaacctt atggaattat acttgtaaca ggacctactg gaagtgggaa aacaacaact
     2941 ttatattctt ctttaaatga acttgataac actcacaaaa aaattatgac tgttgaagac
     3001 cctgttgaat atcaccttaa aaatgttgac cagacacaga taaatccaag ggcaggactt
     3061 acttttgctg ttgctttaag gtctatttta aggcaggacc ccgatgttgt tatggttgga
     3121 gaaataagag acctggagac agcggaaata gcatttagag cagcacaaac aggacaactt
     3181 gttttttcaa ctcttcatac aaatactgct cctggggcaa ttataagatt acttgatatg
     3241 ggagttgaac cataccttat ttcttcttct ttaattgcta tattgaatca gcgacttgtt
     3301 agaagtatat gttctggctg taaatcggaa tacaagccaa ttgatgagga aattaaaaca
     3361 cttgacccaa ctcttttagg taaagatatt aaatactata caggaagagg atgtaattta
     3421 tgtaatggaa ctggttttgg aggaagaatt ggaatttttg aattgatgat aatgaatgaa
     3481 gaattgagaa gattaacaat gagaaaaagt gatttaaggg atataatgga agaagcaaga
     3541 aaaactggaa tgcgaatact tagagaagat ggggttataa aagtaattga aggtataacg
     3601 acaataaatg aagtaattta ctcaacaaga aaggactcag aatgaatata tttaaaaat
//
LOCUS       DAOOVW010000086         3562 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_14161_length_3562_cov_8.812090, whole genome shotgun sequence.
ACCESSION   DAOOVW010000086 DAOOVW010000000
VERSION     DAOOVW010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14161_length_3562_cov_8.812090"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="PLW95_08010"
     CDS             <1..939
                     /locus_tag="PLW95_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOV22599.1"
                     /translation="LLQFIPLIIGMTLISFIIIQLAPGDYFSKLKMNPEISPQTIEQM
                     RRSFGLDKPVIVQYFYWLKNILTFNLGTSFSYHIPVSFLIKQKLKNTLALSIFSMFLT
                     WIIAIPLGIFAAIKNGRWQEKLFSFIAYIGISLPSFFIAMLLVFLFAKYPVLPIGGTR
                     SIFVYRSTFEMFGDYLRHLLVPGIALTLAGIGSLYRLMKNNFLSALNSPYIITARSKG
                     LPEYKVYYKHALRNAINPMITIFGYELSGILSGAGLVEIVTGWPGMGQLILEGVLSQD
                     LYLVMASLLISGLLLIAGNLIADILIAFADPRVRLR"
     gene            936..1994
                     /locus_tag="PLW95_08015"
     CDS             936..1994
                     /locus_tag="PLW95_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012674697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOV22600.1"
                     /translation="MRSFFYKLRRKNIVAFISLVIIILLYVFSFLTDFIAPYPFDKQF
                     RDFSLSPPTRIHFIDENRKFHIIPFVYESKVINPITNKYEEDRSKIYQVKFFVKGEKR
                     KILGFIPFNYCLFGTEGDGKIFLFGTDIHGRDVFSRILYGSRISLSIGLIGVSISFIL
                     GLLLGGISGYFGGKVDNFLMRLVEIIMMFPSFYLLLALRGTFPTDISTVKIYFLIVII
                     LSFIGWASLARIIRGMVLSIKEEEFILSARALGLSTFRIITKHIIPNTFSYTITAITL
                     SIPGYILGESALSLIGLGIQEPFPSWGNMLSVSIGNLKILTSAPWMLIPGFFIFLTII
                     CFNFLGDGLRDLMDPGLK"
     gene            2467..3426
                     /locus_tag="PLW95_08020"
     CDS             2467..3426
                     /locus_tag="PLW95_08020"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV22601.1"
                     /translation="MVLEIKNLVVSLDTFSKRIEILRNVNFSIGKNEVVSLVGESGCG
                     KTFTALSIMKLLPKNAKIEKGEIFIEGENITEKSEKDIAKIRGNKVSMIFQEPSSYLN
                     PVFTIGNQMTECIKEKINKKEKEEKIFKVLKEVNLSEDVFFQYPHQLSGGMQQRVMIG
                     MAIINSPSLLIADEPTTALDVTTAFHIINLLKKLIKTHNISVLFITHDICLAINFSDK
                     IYVMYAGKIVEKASSKKIISSPLHPYTEKLILCLPEKYKKGERIKAIPGNVPDFSSLP
                     SGCAFHPRCPYTKQICKNFSPAEVKIGENYVRCFKYGEIVENN"
BASE COUNT         1188 a          528 c          616 g         1230 t
ORIGIN      
        1 ctattacaat tcataccttt aattatcggg atgacactta tttcctttat tattattcaa
       61 ttagcccctg gtgattattt ttctaaacta aaaatgaacc ctgaaatctc cccacaaaca
      121 atagaacaaa tgagaaggtc atttggactt gataaaccag ttatagtcca gtatttctat
      181 tggttaaaaa atatcttaac ttttaattta ggaacttctt tttcatatca tattcctgtt
      241 tcatttctta taaaacaaaa acttaaaaat acccttgctc tttcaatttt ttcaatgttt
      301 ttgacatgga taattgcaat acccttagga attttcgctg caattaaaaa tggaagatgg
      361 caggaaaaat tgttttcttt tattgcttat attggaattt ctttaccttc tttttttata
      421 gcaatgcttc ttgtattttt atttgcaaaa tatccagtac taccaattgg agggacaaga
      481 agcatatttg tttacaggtc aacatttgaa atgtttggtg attatttaag acatctgctt
      541 gttccaggaa ttgccttaac acttgcaggt attggttctc tttatcgtct tatgaaaaat
      601 aatttccttt ctgctttaaa ttccccatac attattactg ctcgttcaaa gggattacct
      661 gaatataagg tctattataa acatgcttta agaaatgcaa taaatcctat gataacaatt
      721 tttggatatg aactttctgg aattttatca ggtgcaggat tggttgaaat tgtaactggc
      781 tggcctggta tgggacaact tatacttgaa ggagttcttt cacaggactt atatcttgtt
      841 atggcttctt tattgataag tggacttctt ttaattgctg gaaatttaat tgcagatatt
      901 ttgattgctt ttgctgaccc aagagtgagg ttaagatgag aagttttttt tataagttga
      961 gaaggaaaaa tattgttgct ttcatttcat tggttattat tattcttctt tatgtatttt
     1021 cttttttaac tgatttcatt gccccctacc cttttgataa acaattcagg gacttttctt
     1081 tatctccacc aacgagaatt cattttattg atgaaaacag aaaatttcat ataatccctt
     1141 ttgtatatga atcaaaagta ataaatccaa ttacaaacaa gtatgaggaa gacagaagta
     1201 aaatttatca agttaaattt tttgttaaag gagaaaaaag aaaaatatta ggttttatcc
     1261 cttttaatta ttgccttttt ggcacagaag gagatggtaa aattttcttg tttgggacag
     1321 atatacacgg tagagatgtt ttttcaagaa ttctttatgg gagcagaatt tctctttcaa
     1381 taggactgat tggtgtttca atttctttta tacttggtct tcttttaggg ggtatttcag
     1441 gttattttgg tgggaaagta gataatttcc ttatgcgtct tgttgaaata attatgatgt
     1501 tcccttcttt ttatcttctt cttgctttaa gagggacttt cccaacagat atttcaactg
     1561 tcaaaattta ttttttaatt gttataattt taagttttat tggatgggca tctcttgcaa
     1621 gaataataag aggaatggtt ttatcaataa aagaagaaga atttatttta tctgcaagag
     1681 cattgggact ttcaacattt agaattataa caaaacatat aatcccaaat actttttctt
     1741 atacaattac tgcaataact ctttcaatac ctggatatat tctcggagaa agtgctttaa
     1801 gtttaatcgg gcttggcata caggaaccat ttccaagttg gggaaatatg ctttctgtct
     1861 caataggaaa tttaaaaatt ctgacatctg ctccatggat gctgatacca ggttttttta
     1921 tttttttaac aataatatgt tttaattttt taggagatgg gttaagggat ttaatggacc
     1981 ctggactaaa ataaagggct ccccctcacc ctcttcctct ccccgcaggg gagaggagag
     2041 aaaggggtaa aagatatatc cttctccccg atggggagaa ggctcggatg agggggattt
     2101 aatattttcc cctttgacaa accgactaag gaagcaaagg gtagcaaagc agagttggtg
     2161 ctttttccct tttaattctc ccctttagca aaggggagtg agaggggatt ttggagaatt
     2221 acagaaaatc catcccctcg ggcatagccc tacgggcgag cccaccttct cccctccgcc
     2281 actgatacgt tggcggacta tcgcactaag acattgacgg acatgctttg ataaagaaag
     2341 gagttaaaat ttcccccttt tgcaaaccga ctgaagttgc ttaccttgtt tctcagagtt
     2401 ggtgcgttat cgtcttccag atagcacaac ggggataaag gaggatttgg aaggagtatt
     2461 taaattatgg tacttgaaat aaaaaattta gtagtatcat tggatacatt ttctaaaaga
     2521 atagagatat taagaaatgt gaatttttct attggaaaaa atgaagttgt ttctcttgta
     2581 ggtgaaagtg gttgtggaaa aacatttacc gctttatcaa taatgaaact tcttccaaaa
     2641 aatgcaaaaa tagaaaaagg cgaaattttc attgaaggag aaaacataac agaaaaaagt
     2701 gaaaaagaca ttgcaaaaat aaggggaaac aaagtaagta tgatttttca agaaccatct
     2761 tcatacctaa atcctgtttt tacaattggt aatcaaatga cagaatgtat aaaagaaaaa
     2821 ataaataaaa aagaaaaaga agaaaaaatt tttaaagttc tcaaagaagt gaacctgtca
     2881 gaagatgttt tttttcagta tcctcatcaa ttaagtggag gaatgcaaca aagagttatg
     2941 attggtatgg caataataaa cagcccttcc ctcttaattg cagatgaacc aacaactgcc
     3001 cttgatgtaa caactgcttt tcatattatt aacctgctta aaaaacttat aaaaacacat
     3061 aatatttcag ttctttttat tactcatgat atttgtcttg ctataaattt ttcagataaa
     3121 atttatgtta tgtatgcagg gaaaattgta gaaaaggcat cttcaaaaaa aataatttct
     3181 tctcccttgc atccgtatac tgaaaaactt attttatgtc ttcctgaaaa atacaaaaaa
     3241 ggtgaaagaa ttaaagcaat accgggcaat gttcctgatt tttcttctct tccttctggt
     3301 tgtgcatttc atccaagatg tccatacaca aaacaaatat gtaaaaattt ttctccggca
     3361 gaggtaaaaa taggagaaaa ttatgtgagg tgctttaaat atggagaaat tgtggaaaat
     3421 aattgacttg gaaaaaagtt ttgtaataaa agataaatat gggatgacca aaaaaattga
     3481 tgcattgaaa aatataaata ttgatataga aaaaggggaa acacttggta taattggtga
     3541 aagtggaagt ggtaaaacaa ca
//
LOCUS       DAOOVW010000087         3413 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_14945_length_3413_cov_9.021739, whole genome shotgun sequence.
ACCESSION   DAOOVW010000087 DAOOVW010000000
VERSION     DAOOVW010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14945_length_3413_cov_9.021739"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(284..2767)
                     /locus_tag="PLW95_08025"
     CDS             complement(284..2767)
                     /locus_tag="PLW95_08025"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOV22602.1"
                     /translation="MGKKYMWVIGCLLFLCFQFEIYSQSNIDKMKRNEILELSNNGRT
                     AYVIITAEKTIPAEDYAAKELSEHFYKITGAKFPIISEKEKLFSSKGIYVGQTEFAAS
                     KGINFSELNEEEWIIKNYGKDLVLSGGRPRGTLYAVYEFLEQGIGCHWFDGYMEVIPD
                     IPNLCIKEQDIHNKPIFWDRDIYTACPENENDFILRARNKDTRPKPAKFGFGYPLGTH
                     TFYSYSRNFPEDKLEYHAMDSAGNRPKATSPSGPGQICLTNPDARENVLQQIEKSIEK
                     NLKSTEKSTDGSRPQLVYFINPNDTPWICQCPDCKSLAEKEEADSGPYIDFINYIADS
                     IKEKYPEVLIEGWAYANTMKPPKTIYPKDNVVIRIIQLKGEWAVDAIGKDNFSQWSPA
                     WYPDIFRPRIHYANYRANEVLLGWEKISQHLAVWDYWRLYRQQFLSPYINLKSIYYDL
                     QLFGCKKVGSMFAEDEICFDSFFWLKTWAGWKLMQNPYQDIDKLVKIFMDGYYGPAAE
                     KMTSYLNYMEDSIDAVPQESGNMSSMRTFERPYLTFEFYKTCEHLLDEAEALCETGSP
                     YLLNVQRERIPVDAGIIAMWHILGYQLPVGESMPWDKSFLIERYRKNCLNQIEQRHFH
                     GNKESVENDIKKLIEISESYKGVFLRPLVSTGENNLLKNGNFSEGLNGWTYPESELVI
                     VPEIDRLETCSGGACIVGRGIEGKQFLVSQQVEPVKGINKYRLSGWIKIKDFTNLWQA
                     HIQATISLRNLDGKIVNKVVADCNTHHEINNMGWHFRGKEFEVPKDESIISISVTIFT
                     EHPSGKGPKESRPNRGTVWFDEIVLEPVI"
     gene            complement(2897..3262)
                     /locus_tag="PLW95_08030"
     CDS             complement(2897..3262)
                     /locus_tag="PLW95_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22603.1"
                     /translation="MKNKIGVVVLVIIIIAALGFIVKQAMPKKTLYTVVLIDVEAKKA
                     FQKSFDTDAEQIHYPVQSPYTGKNTAYPALKCMQCGAIFPYILEESASVTMPKCPKCS
                     SSEVRALELPKGTKEIDVN"
BASE COUNT         1014 a          658 c          456 g         1285 t
ORIGIN      
        1 atgatataat aaacttactt agtgcctaat tatggcggaa tttggaacca tagataagat
       61 tcactccata cttttttaaa tcctttaaac gcaatttttt tactttttgg tctgcataag
      121 tgagaactct cactcttccc cttgtaaaaa aattactatt atccaaataa agatattttt
      181 cattaaataa ttctggcact tcttttatct ttttttactt tatttccttt tacttttgag
      241 aaaaacaaat agcggaattg agatgaaaaa aattacttcc attctaaata acaggttcaa
      301 gaactatttc atcaaaccat acagttcctc tatttggtct tgattctttt ggacctttac
      361 cacttggatg ttctgtaaaa attgtaacac taatggatat tattgattca tccttgggaa
      421 cttcaaattc cttacctcta aaatgccatc ccatattatt tatttcatga tgagtattac
      481 aatcagcaac tactttgtta acaattttcc cgtctaaatt ccgcaatgaa atagttgcct
      541 gaatatgtgc ctgccataaa tttgtaaaat cttttatttt aatccatcct gataaacgat
      601 atttatttat tccttttact ggttcaactt gttgactgac aagaaattgt ttaccttcta
      661 tcccccttcc tacaatacag gcacctccag agcatgtttc caatcggtct atttcaggaa
      721 caataacaag ttcactttct ggatatgtcc agccatttaa tccttctgaa aaattcccat
      781 tttttaagag attattttcc cctgtggaaa ccaacggtct taaaaatact cctttataac
      841 tttctgatat ttctattaac tttttaatat cattttctac tgattcttta tttccatgaa
      901 aatgtcgttg ttctatttgg ttaagacagt tctttctata tctctcaatc aaaaatgatt
      961 tatcccaagg catagattca cccactggta attgatatcc caatatatgc cacattgcaa
     1021 taatacctgc atcaacagga atcctttctc tttgtacatt caacaaataa ggactgccgg
     1081 tttcacaaag tgcctctgct tcatcaagta aatgctcaca ggttttataa aactcaaaag
     1141 ttagataagg gcgttcaaaa gttctcatac ttgacatatt tcctgattct tgagggactg
     1201 catctatact atcttccata taattaaggt aggaagtcat tttttccgct gcagggccat
     1261 aatatccatc cataaaaatt ttgaccaatt tatcaatatc ctggtaagga ttttgcatta
     1321 gtttccatcc tgcccatgtt tttaaccaga aaaaactatc aaaacaaatt tcatcttctg
     1381 caaacatact ccctactttt ttacaaccaa ataattgtaa gtcataatat atacttttca
     1441 aattgatata aggagaaaga aattgttggc gataaagacg ccaataatcc caaacagcaa
     1501 gatgttgtga aattttttcc caccccaaca atacctcatt tgctctataa tttgcataat
     1561 gaattcgtgg gcgaaatata tctggatacc acgctggtga ccattgagaa aaattatctt
     1621 ttcctatagc atctactgcc cattctcctt taagttgtat tatccgaata actacattat
     1681 cttttggata tattgtctta ggaggcttca ttgtatttgc atatgcccat ccttcaataa
     1741 gtacttctgg atatttctct tttatgctat cagcaatata atttatgaag tcaatatatg
     1801 gtccactatc tgcttcttct ttctcagcca atgatttgca atcagggcac tgacatatcc
     1861 aaggagtatc atttgggtta ataaaataca caagctgtgg tctacttcca tctgtggatt
     1921 tttcagtaga tttcaaattt ttttctatac ttttttctat ttgttgtaga acattttctc
     1981 gtgcatcagg gtttgtaaga caaatctgtc cagggcctga aggagaggtg gcttttggtc
     2041 tatttcctgc actatccatt gcatgatatt ccaatttgtc ttctggaaaa ttgcgcgaat
     2101 aactataaaa tgtgtgtgtc ccaagtggat atccaaaacc aaattttgct ggcttaggtc
     2161 tagtatcttt gtttcttgca cgtaatataa aatcattctc attttctgga caagcagtat
     2221 aaatatctct gtcccagaat attggcttat tgtgaatatc ctgttctttt atacataagt
     2281 taggaatatc aggaataact tccatatatc catcaaacca gtgacaaccg attccttgtt
     2341 ctaaaaattc gtatactgca taaagtgttc cgcgtggacg ccctcccgaa agaacaaggt
     2401 cttttccata attttttatt atccattctt cttcattaag ttctgaaaaa ttaatcccct
     2461 tgcttgctgc aaattcggtt tgtccaacat aaataccttt tgaagagaat agtttttctt
     2521 tttctgatat tattggaaac tttgcacctg taattttata aaaatgttca ctcagttctt
     2581 ttgctgcata atcctcagca ggtattgttt tttctgcggt tattattaca taagcagtcc
     2641 ttccgttatt tgataattca aggatttcgt ttcttttcat tttgtcaata tttgattgtg
     2701 aatatatctc aaattgaaaa caaaggaata ataaacaacc tattacccac atatattttt
     2761 tccccataaa tatccccctt atgtaaaatt tataaaaaga aatagatatc taactttctt
     2821 tattactgct cgctatgttt ttgccctttc agtccaatat gagagtttaa atttttcccc
     2881 ttaattacat ttttatctaa tttacatcta tttcttttgt cccttttggt aattcaagtg
     2941 ctcttacttc tgatgaacta cattttggac attttggcat tgttacagat gcagattctt
     3001 caagtatata gggaaatatt gccccgcatt gcatacattt taaagcaggg taagcagtat
     3061 ttttacctgt ataaggggat tgtacaggat aatgtatttg ttctgcgtct gtatcaaatg
     3121 atttttgaaa agcttttttc gcttcaacat caattaaaac tactgtataa agtgtcttct
     3181 ttggcattgc ttgtttaacg ataaatccta atgcagcaat aattataatt actaatacta
     3241 caactcctat tttgtttttc atattcttat ctccttttga ttttattttc tctaacttat
     3301 ttcttgtcta ccaaggataa ttagcccctg cacaatctag accaaccctc acagacagtg
     3361 gcagtggttg aatattatat aggtctttaa tacctatatt ccttacactt cca
//
LOCUS       DAOOVW010000088         3068 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_17196_length_3068_cov_9.003983, whole genome shotgun sequence.
ACCESSION   DAOOVW010000088 DAOOVW010000000
VERSION     DAOOVW010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17196_length_3068_cov_9.003983"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(213..1484)
                     /locus_tag="PLW95_08035"
     CDS             complement(213..1484)
                     /locus_tag="PLW95_08035"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOV22604.1"
                     /translation="MVTVVVGTQWGDEGKGKVIDYLAKDMDIVARYQGGANAGHTVII
                     NGKKFIFHLVPSGILYPEKICLIGNGTVIDPVSLFEEIAFLEKNGIKVDDRLFIYENA
                     HITMPYHKILDQIEDKFRGKGELGTTGRGIGTTYADKYGRIGIRVVDLLDDDVFYEKL
                     KIALELKNYLLKEYYKEKILNADKIYNDYKEIREKIKKYIINGTIFINDMLSKGKNIL
                     AEGAQGTFLDIDFGTYPYVTASNPISGGSCTGLGISPTKIDKVIGIVKAYTTRVGKGP
                     FPTEMKGLLEDTLRRSGNEYGATTGRPRRCGWFDSVIVRFASIVNGLDEIFITKLDVL
                     SGIEKLKIGVAYKYDGKIYNEYPMNMKVFSNCEVIYEEMDGWSESISDIKEYSKLPTN
                     AKKYIERIQELIEVKISAISVGSEREQIIKI"
     gene            complement(1486..2568)
                     /locus_tag="PLW95_08040"
     CDS             complement(1486..2568)
                     /locus_tag="PLW95_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HOV22605.1"
                     /translation="MGKKITLIPGDGIGPEITEVVKNCIDATGVDIEWDIKEVGLEAL
                     EKYGTPIPDETLMSIKENKVCLKGPITTPVGYGYRSINVFIRQYFNLYVCLRPFKIYE
                     GAKTKFQNVDLVIVRENMEDLYAGIEFEKGKENTLELIEFIEKKTGSKIENDSGISIK
                     PISVKNSERIVRFAFEYARKNRRKKVTAVHKANIMKFTDGLFLDVARKVADEYKDIEF
                     EDKIVDNMAMQLVQKPEIYDVLVCPNLYGDILSDLCAGLVGGLGLSAGANLGEDIAVF
                     EPTHGSAPKYKGQNKVNPSAMILAGILMLDYIGYSDESKKLENALASVIKEGVFVTYD
                     FKEKDDNSSVGTKEMGDAIIRKLKGG"
     gene            complement(2570..>3068)
                     /locus_tag="PLW95_08045"
     CDS             complement(2570..>3068)
                     /locus_tag="PLW95_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOV22606.1"
                     /translation="LKGYVHKFGDDINTDYIISGKYKFKTLDMNELATHLMEDIIPDF
                     YKKIKKGDFIVAGNNFGCGSSREQAPLVIKHSGISGVIAKSFARIFFRNSINVGLVLI
                     QADTDKIDDGDEIEVDVEKGIVRDITKGIEIKSSSLPSFMIDILNEGGVVNYIKKYGS
                     FKIEE"
BASE COUNT          949 a          619 c          300 g         1200 t
ORIGIN      
        1 ttcttaatcc acttacatct tcttccaatt tttcaagttt aggagcaatt actttttgaa
       61 gaaaagaaca tctactctca ttttttcatc tctattttta ttatacttgc ttctttgttt
      121 cctatcaagc attaattagg tacgggtacg gttcttatag agcaccaaaa ccttttattt
      181 ttcaataata ccaaatttta ataaaaaata acttatattt taattatctg ctccctctca
      241 gaaccaacag aaattgcaga tatctttact tcaataagtt cctgtattct ttcaatatat
      301 ttctttgcat ttgttggtaa tttactatat tcttttatgt cagatatact ttcactccat
      361 ccatccattt cttcataaat aacctcacaa tttgaaaata ctttcatatt cattggatat
      421 tcattataaa ttttaccatc atatttatat gcaactccta ttttcaattt ttcaatacct
      481 gataaaacat ccaattttgt tataaaaatt tcatcaagtc catttacaat agaagcaaac
      541 cttacaatta ctgaatcaaa ccaaccacat cttcttggcc ttcctgttgt tgcaccatat
      601 tcattccctg accttctcaa tgtatcttca agaagccctt tcatctcggt aggaaatgga
      661 ccttttccaa ctcttgttgt atatgcttta acaattccta tcactttatc tatttttgtt
      721 ggggaaattc caagacctgt acaactcccc ccagatattg gatttgaagc agtaacataa
      781 ggatatgtac caaaatcaat atcaagaaat gttccctgtg ctccttcggc aagaatattt
      841 tttcccttgg agagcatatc atttataaaa attgttccat ttattatgta tttttttatt
      901 ttctctctta tttctttata atcattataa attttatctg cattaagtat tttttcttta
      961 taatattctt ttaagagata gttctttaat tcaagagcaa ttttaagttt ctcataaaaa
     1021 acatcatcat caagtaagtc aacaactctt attcctattc taccatattt atctgcataa
     1081 gttgtcccta tccctcttcc agttgttcct aattctcctt ttcctctaaa tttatcttct
     1141 atctggtcaa gaattttatg gtatggcatt gtaatatgag cattttcata aataaaaaga
     1201 cggtcatcta cttttatccc atttttttca agaaatgcta tttcctcaaa taaagaaaca
     1261 gggtcaataa ctgtcccatt accaattaaa caaattttct cgggataaag aataccagat
     1321 ggaacaagat gaaaaataaa ttttttacca tttataatta ccgtatgacc tgcatttgct
     1381 cctccctgat atcttgcaac aatatccata tcttttgcaa ggtaatcaat aactttccct
     1441 ttcccttcat ctccccattg tgttccaaca acaactgtaa ccatttcaac ctccttttaa
     1501 tttccttata attgcatcgc ccatttcttt tgtcccaacg gaagagttat catctttttc
     1561 tttaaaatca taggtaacaa aaacaccttc ttttattaca cttgcaagtg cattttcaag
     1621 ttttttactt tcatcagaat aaccaatata atcaagcatc aaaatacctg ctaatatcat
     1681 agcagaagga tttactttgt tttgtccttt atattttgga gcagacccat gcgttggctc
     1741 aaaaactgct atatcttctc ccaaatttgc acccgcagaa agtcctaatc caccaacaag
     1801 ccctgcacaa aggtcagaaa gaatatcccc ataaagattg ggacaaacaa gaacatcata
     1861 aatttctggt ttttgaacaa gttgcattgc catattatca acaattttat cttcaaattc
     1921 aatatcttta tattcatctg caactttcct tgcaacatca agaaaaagtc catcagtaaa
     1981 tttcattata tttgctttat gaacagcagt tacttttttt cttctatttt ttcttgcata
     2041 ttcaaaagca aatctgacaa ttctctctga atttttaact gagattggtt ttatgcttat
     2101 tccagaatca ttttctattt tacttcctgt ttttttctca ataaattcaa tcaattcaag
     2161 tgtattttct ttcccttttt caaactcaat tcctgcataa aggtcttcca tattttctct
     2221 aacaattaca aggtcaacat tttgaaattt tgtttttgca ccttcataaa ttttaaatgg
     2281 tcttaaacaa acatataagt tgaaatattg tcttataaaa acatttatac ttctatatcc
     2341 atacccaaca ggtgttgtta taggaccctt caggcatact ttattttctt ttatagacat
     2401 taatgtttca tctggaatag gagttccata tttttcaagt gcttccaatc caacttcttt
     2461 tatatcccat tcaatatcaa ctcctgtggc atcaatacaa tttttgacaa cctctgtaat
     2521 ttctggtcct attccatctc ctggaattaa tgtaattttt tttcccatat tactcctcta
     2581 ttttaaaact accatatttt tttatgtaat taacaacacc accttcattt aaaatatcaa
     2641 tcataaatga tggcaacgaa gaacttttta tttctattcc ttttgtaata tctctaacaa
     2701 tacctttttc tacatctact tcaatttcat ctccatcatc tattttatca gtatctgcct
     2761 gtattaaaac aagaccaaca tttatgctat ttctaaaaaa tattcttgca aaactttttg
     2821 caatcacacc tgatatacct gaatgtttta ttacaagagg tgcttgttct ctacttgaac
     2881 cacacccaaa attatttcct gcaacaataa aatcgccttt tttaattttt ttatagaaat
     2941 caggaattat atcttccata agatgtgttg caagttcgtt catatcaagt gttttaaact
     3001 tatattttcc tgaaataatg taatcagtat ttatatcatc accaaattta tgaacatatc
     3061 cttttagt
//
LOCUS       DAOOVW010000089         2572 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_21837_length_2572_cov_7.328566, whole genome shotgun sequence.
ACCESSION   DAOOVW010000089 DAOOVW010000000
VERSION     DAOOVW010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21837_length_2572_cov_7.328566"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..2054
                     /locus_tag="PLW95_08050"
     CDS             48..2054
                     /locus_tag="PLW95_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22607.1"
                     /translation="MFIRYYLPENKEERLGELVEFCKKTETDGILFFTSSYDSQPSFI
                     DIEKYRNYAKKLKKWAILIKKENLRFGINMLQTLGHIYFPKKIERKFPFQRKVDINGI
                     ESKGGCCPLDENFKKYIYEVYQVFASLKPDIIYVDDDFRYIMDGFFCFCPLHLKKYEK
                     ETGEKITREKLKEEILKETFIPSDIKKKYFEILNLSLKEVAELIEKAVHSISPETRVG
                     LMLQTFPMCFWGTDINEIIKTLAGNRQPLCRPQLPIYKEYGFDLKLSIPLSFAQPLII
                     RNLINKDVEIQPEIENYPYSIFSKSKQIFKYQISFCYINGFEKPLVNIFDTYGQKFDE
                     NIEFIEVLKKNKKFFGKLKEISKNNVSKGISIIHTSKTTLFTTPQNKNLYTIFNSLCW
                     DRIIPLLGLPISYNWNEPEFYILSGNEVLGLEKEKLEQIIKNGGVIIDKDALQILQYL
                     GYEKKIGVKCREAVPIDDCGSIYFHKNKINGPYQDEFFPVRFATFPKDFVNLEILSEK
                     FETIASLVNFENKIVSPAIVITENKYGAKFGIFSFVPTENLLPVFLNPKTQVLLRNLF
                     PEINGKSLPISITNHPYLVPYYFEGKNGIFILILNLSSDKVSNIVLNSQNIFNKKCFK
                     PYLLLGNGKFIEFNKKVKFEKKENIIKIQYNLSPFEFLIMSFRK"
     gene            2062..2235
                     /locus_tag="PLW95_08055"
     CDS             2062..2235
                     /locus_tag="PLW95_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22608.1"
                     /translation="MNNVYLKRYEKNPIMEIKKGFILKKVAVIFVQRIPSGVDPTGEP
                     LDKIVILKEIGKN"
BASE COUNT         1076 a          276 c          375 g          845 t
ORIGIN      
        1 agtagttatt ttgttaaaga taaatacttt cttatatttt aataaaaatg tttattagat
       61 attatttacc tgaaaataaa gaggagagat taggagaatt agttgagttc tgtaaaaaaa
      121 cagaaacaga tggaatttta ttttttactt caagttatga cagtcaacct tcatttatag
      181 acattgagaa atatagaaat tatgcaaaaa aacttaaaaa atgggcaata ttgataaaaa
      241 aagaaaatct aagattcggg ataaatatgt tacaaacact tggacatata tatttcccaa
      301 aaaaaataga gagaaaattc ccttttcaaa gaaaagttga tattaatgga attgaatcta
      361 aaggaggatg ttgtccatta gatgaaaatt ttaaaaaata tatatatgaa gtttatcaag
      421 tttttgccag tttaaaacca gatattattt atgtcgatga tgattttaga tatattatgg
      481 atggtttttt ttgtttttgc cctcttcact taaaaaaata cgaaaaagaa acaggtgaaa
      541 aaattactcg tgaaaaatta aaagaagaaa tattgaaaga aacttttatt ccatctgata
      601 ttaaaaagaa atattttgaa attttaaatt tatccttaaa agaagtagca gaattgatag
      661 aaaaagcagt tcatagtata tcaccagaaa caagagtagg gttgatgttg cagacatttc
      721 caatgtgctt ttggggaact gacataaatg agattataaa aacattagca gggaataggc
      781 aacctttatg cagaccacaa ctgccaatat ataaggaata tggatttgat ttaaaattat
      841 caattccatt atcttttgct cagccattaa taataagaaa tctaataaat aaagatgttg
      901 aaattcaacc agaaatagaa aattatccat actcaatatt ttcaaaatca aaacaaattt
      961 ttaaatacca aatttctttc tgttatataa atggatttga gaaaccactt gtaaatattt
     1021 ttgatactta tgggcagaaa tttgatgaaa atattgagtt tatagaagta cttaaaaaaa
     1081 ataaaaaatt ctttggaaaa ttaaaggaga ttagtaaaaa taatgtatcc aaaggtatat
     1141 caataattca tacttctaaa acaactttat ttacaacccc ccaaaataaa aacctttata
     1201 caatttttaa ctcattatgc tgggatagaa taatacctct tcttggttta cctatttcat
     1261 ataattggaa tgaaccagag ttttatattc tttcaggaaa tgaagttctt gggttagaaa
     1321 aagaaaaatt agaacaaata attaaaaatg gaggtgtgat aattgataaa gatgctttac
     1381 aaatacttca atatctcggt tatgagaaaa aaataggagt taaatgtagg gaagcagttc
     1441 ccattgatga ttgtggaagt atttattttc ataagaataa aataaatggt ccttatcagg
     1501 atgaattctt tcctgtaaga tttgcaactt tcccaaaaga ttttgttaat cttgaaattt
     1561 tgtcagaaaa atttgagaca attgcatctc tggtaaattt tgaaaataaa atagtttctc
     1621 ctgcaattgt tataactgaa aataaatatg gtgcaaaatt tggaattttt tcttttgttc
     1681 ctactgaaaa tttattgcct gtttttctaa atccaaaaac tcaggtacta ttaaggaatc
     1741 tctttcctga aataaatgga aagtcattac ctatatctat aacaaaccat ccttatcttg
     1801 tgccatatta ttttgaaggt aaaaatggaa tttttatatt gatactgaac ctttcatctg
     1861 ataaagtttc aaatattgtt ttgaactccc agaatatttt taataaaaaa tgttttaaac
     1921 catacttgct tttaggaaat ggtaagttta ttgaattcaa caaaaaagta aaatttgaaa
     1981 aaaaagaaaa tataataaaa attcaataca atttatcccc ctttgaattt ttaattatgt
     2041 cttttagaaa ataggagaga tatgaataat gtatatttaa aaagatatga aaaaaaccca
     2101 attatggaaa taaaaaaagg gttcattctt aaaaaagttg cagtaatatt tgtccaaaga
     2161 atcccctcgg gcgtagaccc tacgggcgag cctctggata agatagtgat actcaaagag
     2221 ataggaaaga attaaagtta ggagtgaaga tttggtataa taaagagaaa aaaatggata
     2281 aaaagtatag acagaataaa ctgatgaaaa tactttatca attaagtaag aagaagaaat
     2341 atctctaagc ataaattatt aaaattgtga ggttattaaa atataaatta aatactttaa
     2401 atgcaactta aataagaaag accataaaaa aataaaaaaa gtaaactttt caaaaatttt
     2461 gtcatctaat atataataac tataaaattt ttgaaaaaag gaggaaaaaa tggaaagaac
     2521 gaaaaaaaag atagttgggt tagttgtagg aatagtatta gttttatctt tc
//
LOCUS       DAOOVW010000090         2512 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_22572_length_2512_cov_8.160765, whole genome shotgun sequence.
ACCESSION   DAOOVW010000090 DAOOVW010000000
VERSION     DAOOVW010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22572_length_2512_cov_8.160765"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(465..974)
                     /locus_tag="PLW95_08060"
     CDS             complement(465..974)
                     /locus_tag="PLW95_08060"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOV22609.1"
                     /translation="MEKEEWKEKSIVIQNKFLSTLSLENFSCVLTYVNVDREVKTDLI
                     IKTALSNGKRICVPKIDWIEKIFIPVKILSFSDIDFNKKIPQPKNNDTVSYDEINLSI
                     TPGIVFDIYGNRIGRGEGFYDKFFQNLKNVFKVALAFDFQVLEDKLPVKEYDSKVDLI
                     ITEKRIIRC"
     gene            complement(928..2334)
                     /locus_tag="PLW95_08065"
     CDS             complement(928..2334)
                     /locus_tag="PLW95_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOV22610.1"
                     /translation="MEDLFEPEQQNFERNSLSVKMRPEILDEYVGQQHLLGEGKILRK
                     LIEKDNIPSMILFGPPGCGKTALAFLIAHQTKKPFYPLNAVTSTVSDVREVIKIAREK
                     FSQTGKSTILFLDEISHFNKLQQDALMRDVEEGIIILIGTTTHNPYFYINTPLLSRCT
                     VFELKPLTDEDIERIIKNAIKDRKKGLGNYNLKITEEAIKYIVKSSEGDARKSLNTLE
                     IGVSIAPVNSDGVINFDIDIARECMQKKYLLYDREDRHYDTISAFIKSMRGSDPDAAI
                     YYLAKMIASGEDPKFIARRILICASEDVGNADPQALLIANACYQAVEVIGMPESRIIL
                     AQATIYVATAPKSNSSYLAIEKAIEDVESEKTKVVPEYLQHGGYKGAEELKRGQGYIY
                     PHDYPYHYYPQKYVYGEKNFYIPTDIGYEKRIKSYLNFIERLKKEYEKRGNKERNKEQ
                     KRKDGEGGMEGKKYCNPK"
BASE COUNT          734 a          536 c          347 g          895 t
ORIGIN      
        1 aaatttttca ctctcatccg caccagctcg gcgtaagcaa atcatagtga agtatcaggg
       61 gaaagacaac gccgaggttc caccactccg gctgtaccaa atttataata cattagcctc
      121 aaaaagcaac tttccctcgt cttcctctcc ccggtgggga gaggataggt gaggggactt
      181 tttcttttac attttcctca tttttttctt gctactggct acttttatta aaccccgttt
      241 ccttacattg ctcttacctt gtagggctgg ttcgtatgct ttccttaaac tgctccgcag
      301 gatccctctg ggcatagcca acgggcgagc ctgtggactg tgtagaacaa tcccccctca
      361 accctcgcct tctccccctc tgggagaagg gaaaaacttc ctaacgataa tatattaccc
      421 aaatttttct acttaaaaca ttataacttc ttttctcttt actttcaaca ccttataatt
      481 cttttttcag taattatcaa atccacttta ctatcatatt ccttcactgg caatttatct
      541 tctaaaacct ggaaatcaaa agcaagggca actttaaaca cattttttaa attctgaaaa
      601 aatttgtcat aaaatccctc tcccctccca attctattcc catatatatc aaatacaata
      661 cctggtgtaa ttgataaatt tatttcatca taagagacag tatcattatt ctttggttga
      721 gggattttct tattaaaatc aatatctgaa aaagatagaa ttttcacagg aataaaaatc
      781 ttttctatcc agtcaatttt gggaacacat attcttttac catttgagag agccgtttta
      841 ataattaaat ctgttttaac ttctctatca acattgacat aagtaagaac acaggagaaa
      901 ttttctaatg acaaagtact taaaaactta ttttggatta caatactttt ttccttccat
      961 tcctccttct ccatcctttc tcttttgttc tttatttctt tccttatttc ctcttttttc
     1021 atattctttt ttaagtcgtt caataaaatt taaataactt tttattcttt tttcataacc
     1081 aatatctgtt ggaatataaa aatttttttc tccataaaca tatttttgag gataataatg
     1141 atatggataa tcatgtggat agatatatcc ctgccccctt tttaattctt ctgctccttt
     1201 atacccgcca tgttgaagat actcaggaac aactttagtt ttttcgcttt caacatcttc
     1261 aattgctttt tcaattgcaa gatatgatga attactttta ggtgcagtag ccacataaat
     1321 tgttgcctgt gcaagaataa tcctgctttc tggcatacct ataacttcaa ctgcttgata
     1381 gcaagcattc gcaattaaaa gtgcttgtgg gtctgcatta ccaacatctt cacttgcaca
     1441 tatcaaaatc cttcttgcaa taaatttagg gtcttcacca ctcgcaatca tctttgctaa
     1501 ataataaata gcagcatcag ggtcagaacc acgcatactt ttaataaaag cagaaattgt
     1561 atcatagtgc cggtcctctc tatcatacaa gagatacttt ttctgcatac attctctggc
     1621 aatatcaata tcaaaattta taactccatc tgaattaaca ggagcaattg aaacaccaat
     1681 ttcaagagta tttaacgatt ttcttgcatc cccttcactt gattttacaa tatattttat
     1741 tgcttcctct gttattttta agttatagtt tcccaatccc ttctttctat ccttaattgc
     1801 attttttatt attctctcaa tatcctcatc agttaatggt tttaattcaa aaacagtgca
     1861 tcttgaaagt aaaggtgtat ttatataaaa atacggatta tgagttgtag ttcctatcaa
     1921 aattataata ccttcttcaa catctctcat taaagcgtcc tgttgcaatt tattgaaatg
     1981 tgaaatttca tccaaaaata aaatagttga ttttcctgtt tgactgaact tttctcttgc
     2041 aatttttatt acttctcgta catctgaaac agtagaggta actgcattaa gaggataaaa
     2101 tggctttttc gtctgatgtg caataagaaa agcaagggct gtctttccac atcctggcgg
     2161 accaaataaa atcattgaag gaatattatc cttctctata agttttctta aaatcttccc
     2221 ctcacctaat aaatgttgct gtccaacata ctcatcaagt atttcaggac gcattttaac
     2281 tgagagggag ttcctttcaa aattctgttg ttctggttca aaaaggtctt ccattttctt
     2341 taatgtggag gacagtcctc caccaaactt tcaatgggcg gtacaggact tgaacctgtg
     2401 ccgtcgcttt cgctcctggg acaggggtgc ttccccctcg ggcgtagccc tacgggcgag
     2461 cctttcccaa cgataccccc aatacgaaat acattattat aaatatggga aa
//
LOCUS       DAOOVW010000091         2472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_23091_length_2472_cov_4.892429, whole genome shotgun sequence.
ACCESSION   DAOOVW010000091 DAOOVW010000000
VERSION     DAOOVW010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23091_length_2472_cov_4.892429"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="PLW95_08070"
     CDS             <1..1161
                     /locus_tag="PLW95_08070"
                     /inference="COORDINATES: protein motif:HMM:NF014948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HOV22611.1"
                     /translation="FGDERGIADTIRYSQLDVPVFVHAFPDEIDKMDVSNRRDSFCGK
                     ISICNIFYQYGIKFTLSSFHTLTLDEEFKKEIDNFIKICRILKNLKNLKIGAIGTRPV
                     AFKTVRYSEKILENNGISVDTIDLSEIFSKIDKIQNNDIKVKDEFEQLKNYADTSLIS
                     DQAIIKMAKFKIVIENWIREYDIKTVAIQCWNSIEENYGIAPCIVMSYLSNYLIPSAC
                     EVDITGALGMYILQIASEKPSAIVDWNNNYKNEKDKCVLFHCSNFPISFFEENKIVSS
                     EIIKSSVGEENAYGSIIGKIKAGHFTFLRLSTDDIKGEIKAYTGEGEITEEKVKTFGG
                     YGVAEIKNLQKLLKFICENGFEHHTCINFSNVSNIIYEALKKYKKWDIYKND"
     gene            1241..2254
                     /locus_tag="PLW95_08075"
     CDS             1241..2254
                     /locus_tag="PLW95_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOV22612.1"
                     /translation="MTKKERVKYALLHQETDIVPFNINFTQPAYRKLTNYFGGEISED
                     EIGNHIVYIEPVSFNSWVEIKPNMWMDEFGVMWDRSIDKDIGNPIPVLKYPSIKGYKF
                     PDPDNPKRYSHFSTIIEKNKDKFILCSIGFSLFERAWTLRGMENFLIDMIENPPFVNE
                     LLDTIVNFNIKIIKNTSKYPIDGFRFGDDWGQQKGLIMGIPFWRKFLKPRLEKMYQEV
                     HKVGLPVFIHSCGDVQEILPELIDIGVNVFNPFQPEVMNIFEIKRIYGDKLSFFGGIS
                     VQKLLPFGTPQQVKDETKKILQTIGKGGGYIASPSHDIPKDVPVENIVALIETLNPDF
                     PFK"
     gene            complement(2255..>2472)
                     /locus_tag="PLW95_08080"
     CDS             complement(2255..>2472)
                     /locus_tag="PLW95_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22613.1"
                     /translation="KNKVISPIAVPISPHKKITTTKYKNFILVIQYNTFFDFLSTEIF
                     ISSERKKLQVRWGNFTSNTRCIIFIYS"
BASE COUNT          955 a          280 c          395 g          842 t
ORIGIN      
        1 tttggggatg aaagagggat tgcagataca ataagatatt ctcaacttga tgttcctgtt
       61 tttgtccatg catttccaga tgagattgat aaaatggatg tttcaaatag aagagatagt
      121 ttttgcggta aaatttcaat ttgtaatatt ttttatcaat atggaataaa atttacttta
      181 tcctcgtttc atacattaac tcttgatgaa gagttcaaaa aagaaataga taattttatt
      241 aaaatatgta ggattttaaa aaatctaaaa aacttaaaaa ttggagcaat tggaacaaga
      301 cctgttgctt ttaagacagt aagatatagt gagaaaatac ttgaaaataa tggtataagt
      361 gtagatacaa ttgacctttc agaaattttt tcaaagatag ataaaattca aaacaatgat
      421 ataaaagtta aagatgaatt tgaacaactt aaaaattatg cagatacaag tttaatttca
      481 gaccaggcaa ttataaaaat ggcaaaattc aaaattgtca ttgagaactg gataagagaa
      541 tatgatataa aaacagttgc aattcaatgt tggaattcaa tagaagaaaa ttatggaatt
      601 gctccttgca tagttatgag ttatctttca aactatctta ttccttctgc ttgtgaggtt
      661 gatattactg gtgctttagg tatgtatatt ttacaaattg catcagaaaa accttctgca
      721 atagttgatt ggaataacaa ttataaaaac gaaaaagata aatgtgtcct ttttcattgt
      781 agcaattttc ctatatcatt ttttgaggaa aacaaaattg taagttcaga aataataaaa
      841 tcaagtgttg gtgaagaaaa tgcttatggg tcaattattg ggaaaataaa agcaggacat
      901 tttacttttt taagattatc aacagacgat ataaaaggag aaattaaggc atatacaggg
      961 gaaggagaaa tcacagaaga aaaggttaaa acatttggtg gttatggtgt tgcagaaatt
     1021 aaaaaccttc aaaaattatt aaaatttata tgtgaaaatg gatttgaaca tcatacctgt
     1081 ataaatttct caaatgtttc aaatattatt tatgaagcat taaaaaaata taaaaaatgg
     1141 gatatatata aaaatgacta aggtttagag taaacaattt ctctattaca atactttttt
     1201 tgttaaacat aaaaatttta gtttgatagg gaatatgaaa atgactaaaa aagaaagagt
     1261 taaatatgcc ttgcttcatc aagaaacaga tattgttcct tttaatataa attttactca
     1321 acctgcatat agaaaactta ctaattattt tggaggggaa atctctgaag atgaaattgg
     1381 aaatcatata gtttatatag aaccagtttc ttttaactca tgggttgaaa ttaaacccaa
     1441 tatgtggatg gatgaatttg gagttatgtg ggataggtct atagataaag atataggtaa
     1501 tccaatacct gtgctaaaat atccctctat aaaggggtat aaatttcctg accctgataa
     1561 tcctaaaaga tattctcatt tctctacaat tatagaaaaa aataaagata aatttatttt
     1621 atgctctatc ggtttttctc tttttgaaag agcatggaca ttaagaggaa tggaaaattt
     1681 tttaatagat atgatagaaa atccaccttt cgtcaatgaa cttcttgata caattgttaa
     1741 ttttaatata aaaataatta aaaacacttc aaaatatcca atagatggtt ttagatttgg
     1801 agatgactgg ggacaacaaa aagggttgat aatgggaata cctttctgga gaaaatttct
     1861 taaacctcga ttagaaaaaa tgtatcagga agttcataaa gttggcctgc cagtatttat
     1921 tcattcttgt ggagatgttc aagaaatact acccgaatta atagatattg gcgtaaatgt
     1981 ttttaatcca tttcaaccag aagtaatgaa tatctttgaa atcaaaagaa tttatggaga
     2041 taaactttca ttttttggtg gtattagtgt tcaaaaactt cttccatttg gtacacctca
     2101 acaggtaaaa gatgagacta aaaaaatttt acaaactatt ggcaaaggag gaggatatat
     2161 tgcttctcca tctcatgata ttcctaaaga tgttcctgta gaaaatattg ttgctcttat
     2221 agaaacatta aatcctgatt tcccttttaa ataactatga gtaaataaaa attatacatc
     2281 tcgtattact ggtaaaattg ccccatctaa cttgtaattt ttttctttca cttgatataa
     2341 aaatttcagt tgacagaaaa tcaaaaaaag tgttatattg aattaccaaa atgaaatttt
     2401 tatatttagt agttgtgatt tttttatgtg gactgatagg aacagcaatt ggagaaatta
     2461 ctttattttt tg
//
LOCUS       DAOOVW010000092         2458 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_23274_length_2458_cov_6.499376, whole genome shotgun sequence.
ACCESSION   DAOOVW010000092 DAOOVW010000000
VERSION     DAOOVW010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23274_length_2458_cov_6.499376"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..2335
                     /locus_tag="PLW95_08085"
     CDS             38..2335
                     /locus_tag="PLW95_08085"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HOV22614.1"
                     /translation="MNIFKNCGWIWYDHYGYEDVNVFMLARKSFSLRDIPKTAEINIT
                     ADSRYKLYVNGEYVCYGPARGFPESYPFDRIDIKRYLKKGENVIAVVVWQWGHGTFQS
                     IYAGGNGLIVDGKIGKVDIGTKINNGWLVKKCPAHKQDMPRRSVQIGYQEYYDARKIS
                     EDWMLPETNIKFDRTDWQEPTWRKSGCAPWFKFEERGIPLLKEEKKFFKKILHMYYGN
                     NWKNWEKERNLTIIYLNELNGKEDRSRVKDPENMLFDNEKFIEILPFPENKKISFIID
                     YGEEVAGFIGFEIIGNGGEVLDITTTEVCKDGWPFVGDPLSGSKIAMSDRYIARNGKQ
                     QFETFSIHGFRYLCLTIRNIKENFKIKKIYVRSISYPFQEKVVFNSSDETINKIWDMC
                     VRTQICCSFDSYVDCPWREQAQWWGDARVQGANTYYLFGDMRLFRRGIKQAGQSQIEN
                     GLTYGTFPSIAVGCILPDYTLTWVHTHLDYYKYTGDISLLKEQFDKIEKAISFFDKKV
                     EENYLLPPMPEWWVFLDWAPLYKEGFSCLFNLMYLYTLKTMVEICKILKREDRKNLYE
                     EKSNVLEKRIDKIFWDKKKNVYFDGYDIKKKKIIKKISQHTHTYAILLNLKKEYHKKW
                     IEEILLPPMKEYPLKHPTIIEGSPFFYYYIIEVMKKVGGYEKEIIDFIKRRWGKMIEE
                     GATTCWEVWKPEMGLSSLCHAWSAHPAIHFIELIAGVKPIAKKREKVKIKKPQFVENL
                     YFKIKTKTEEIEIVKNGKNVKTKRK"
BASE COUNT         1001 a          253 c          471 g          733 t
ORIGIN      
        1 taaatgaagt aatttactca acaagaaagg actcagaatg aatatattta aaaattgtgg
       61 atggatatgg tatgaccact atggatatga agatgtaaat gtttttatgc ttgcaagaaa
      121 aagtttttcc ctaagagata ttccaaaaac agcagagata aatataacgg ctgattcaag
      181 atataaactt tatgtaaacg gagaatatgt gtgttatgga cctgcaaggg gatttcctga
      241 aagttatcca tttgatagga ttgatataaa aagatattta aaaaaaggag aaaatgtaat
      301 tgctgttgta gtatggcaat ggggtcatgg aacttttcaa tcaatttatg caggtggaaa
      361 tggtcttatt gtagatggaa aaatagggaa agttgatata gggacgaaaa taaacaatgg
      421 ttggcttgta aaaaaatgtc ctgcccataa acaggatatg ccaagaaggt ctgttcaaat
      481 tggatatcaa gaatattatg atgcaagaaa aataagcgag gattggatgt tgcctgaaac
      541 aaacataaaa tttgatagaa ctgactggca agaaccaaca tggagaaaat ctggctgtgc
      601 tccttggttt aaatttgaag aaaggggaat accactttta aaagaagaga aaaaattttt
      661 taaaaagatt ttacatatgt actatggaaa taactggaaa aattgggaaa aggagaggaa
      721 tttaactatt atttatttaa atgaattaaa tgggaaagaa gatagaagca gagtaaaaga
      781 ccctgaaaat atgctttttg ataatgaaaa atttatagaa attttgccat ttccagaaaa
      841 taaaaaaatt agtttcataa ttgattatgg agaagaagtt gctggtttta taggttttga
      901 aattatagga aatggtggag aagttttaga tattacaaca acagaagttt gtaaagatgg
      961 ttggcccttt gtgggggacc cattatctgg aagtaaaata gcaatgagtg atagatatat
     1021 agcaagaaat gggaaacaac aatttgaaac attttcaatt catggattta gatatttatg
     1081 tttgacaatc agaaatataa aagaaaattt caaaataaaa aaaatttatg tgagaagtat
     1141 ctcttatcct ttccaagaga aagttgtttt taactcgtca gatgaaacaa tcaataaaat
     1201 atgggatatg tgtgttagaa ctcaaatatg ttgtagtttt gattcttatg ttgattgtcc
     1261 atggagagaa caggcacaat ggtggggaga tgcaagggtt caaggagcaa atacatacta
     1321 tttatttggt gatatgcgac ttttcagaag aggtataaaa caggcaggac aatcacaaat
     1381 tgaaaatgga cttacttatg ggacttttcc atcaatagca gtagggtgta ttttacctga
     1441 ttatactctt acatgggtcc atacacatct tgattattat aaatatacag gagatatttc
     1501 tcttttgaaa gaacaatttg ataaaataga aaaagcaata agtttttttg acaaaaaggt
     1561 tgaagagaat tatttacttc caccaatgcc tgaatggtgg gtttttcttg attgggcgcc
     1621 tttatataaa gaaggttttt cttgtctttt taatttaatg tatctttata ccttaaaaac
     1681 aatggtggag atatgtaaga ttcttaaaag agaagataga aaaaatttat atgaggaaaa
     1741 aagcaatgta ttggaaaaaa gaattgataa aattttctgg gataagaaga aaaatgttta
     1801 ttttgatgga tatgatataa aaaagaaaaa aattataaaa aaaatatcac aacatacaca
     1861 tacttacgca atcttactta atttaaaaaa agaatatcac aaaaaatgga ttgaagaaat
     1921 actccttccc ccaatgaaag aatacccttt aaaacatcct actataattg agggttcacc
     1981 atttttctat tactacataa tagaagtgat gaaaaaagtt gggggatatg agaaagaaat
     2041 aattgatttt ataaaaagaa gatggggaaa aatgatagaa gaaggagcaa caacttgctg
     2101 ggaagtatgg aaaccagaga tgggtttaag ttctttatgt catgcatgga gtgctcatcc
     2161 cgcaattcat tttatagaac taattgcagg tgtaaaacca attgcaaaaa aaagggaaaa
     2221 agttaaaatt aaaaaaccac aatttgttga aaatttatat tttaaaataa agacaaaaac
     2281 agaggaaata gaaattgtta aaaatggaaa aaatgttaaa acaaaaagga aataagaagg
     2341 aaataataga ttttaataat ccatttacaa agaaaggaaa ttggtataaa ggatgttttc
     2401 atattcatac aacaaattct gatgggactt taactcctga tgaaatttgt aaaattta
//
LOCUS       DAOOVW010000093         2376 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_24416_length_2376_cov_8.499354, whole genome shotgun sequence.
ACCESSION   DAOOVW010000093 DAOOVW010000000
VERSION     DAOOVW010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24416_length_2376_cov_8.499354"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(589..1860)
                     /locus_tag="PLW95_08090"
     CDS             complement(589..1860)
                     /locus_tag="PLW95_08090"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HOV22615.1"
                     /translation="MRREKIIFIISIFLLCFLYSVNGEERQIEAIWTDVRPIIDGKLE
                     GCWQKAAKAENFYDCSELKLADVQTTAYLLYDDKALYIAFYCDEPNINEINRILSYKT
                     RDSRIWENECVEIFLSPYSNGSPYFHFITNVAGTKYDALGTESSWNYLWEVATSIGKD
                     YWVAEFCIPFSSLNLSSDTNQVWRINFNRERKISCENSAWSSTFSEFHKPEKFGVLRG
                     LKFPAPTIHIVSLDERIVWGKNKFKAIIENESQDDWSGVAYLRIEDEERNFPITLAKE
                     QVNIPGKTIKEIQLEKYTRQKDGRNIVSLTLEDANGEVIVSASRTLPIIPLFLQKFFI
                     LYKGEEDLSFEVTAPEMISSANFYILQGKKRVMEKKVKFSTSGKEPTMVAIPTKGLEC
                     GSYSCKIDVFFLQEKTAETLNFNFEIIPGIF"
BASE COUNT          771 a          456 c          355 g          794 t
ORIGIN      
        1 aaatggaaat agaaaaagaa gagaaaaata aaagatgtgt tggagtagat ttttaatgtc
       61 tcaacgaatt agtatttcga gtagattttt atttgacaca acagttacct tgttattacc
      121 atatgattat gtgacatctt ctaccaaata aactcgctaa gaaaataaaa actctcttaa
      181 ttctgaaaac cctcttaaaa taacttcttc aagtttaggt aggtatatta ttgagcaata
      241 agatatctta gatactaaga attaggtcta ctgctaaggg agttcaatta ggatttaaaa
      301 aaatacttat acaaaagaag aaaaaaataa aattgtccaa gaacaaatcc ttaatatcgt
      361 gatgccttac atcttatttg tttttcccca tgatccttca aacaatactt gtgacccttc
      421 gggcgtagtc ataggagaaa ggggagggat tttgtccttt cgttttttat cttgctactt
      481 tctcctctct actttcagtc aacggaaatt gtccaaagac ttctgtggaa ctgagtattt
      541 gcttctactg tccatttttc aagattgcat tgtacaaata tgttgttatc aaaaaatacc
      601 tgggataatt tcaaaattaa aatttaatgt ttccgcagtt ttctcttgca agaaaaaaac
      661 atctatttta caggaataag aaccacactc taaacctttt gtcggtatag caaccatagt
      721 tggttctttc ccagaagtag aaaacttaac ttttttctcc ataacccgtt ttttaccctg
      781 cagtatataa aaatttgctg atgaaatcat ctccggcgca gtaacctcaa aacttaaatc
      841 ttcctctcct ttataaagta taaaaaactt ttgtaaaaaa agtggaatta tcggcagagt
      901 tcttgatgcg gaaacaataa cttctccatt cgcatcttcc aaagtaagag acactatgtt
      961 tctgccatct ttttgtctgg tatatttttc taactgaatc tcttttattg ttttacccgg
     1021 tatatttacc tgctcttttg ccaatgtaat cgggaagttt ctttcttcat cctctattct
     1081 caaatacgct acaccagacc aatcatcctg gctttcgttt tcaataattg ctttgaattt
     1141 attctttccc caaacaattc tctcgtctaa acttacaata tgaatagttg gtgctggaaa
     1201 tttaagacct ctaagcacac caaatttctc tggcttgtga aattcactga aagtagaaga
     1261 ccatgcacta ttttcacatg aaatctttct ctccctgttg aagtttattc tccatacctg
     1321 attggtatcc gaagaaagat ttaaagaaga aaaaggaata caaaattcag caacccagta
     1381 gtccttacca atagatgtag caacttccca aagataattc caagaagatt ctgttcctaa
     1441 agcatcatat tttgtcccag caacattggt tataaaatga aaataagggc tcccatttga
     1501 ataaggggaa agaaatatct ctacacattc attttcccaa attctgctgt ctctggtttt
     1561 ataggaaaga atcctattta tttcgtttat attaggttca tcacaataaa aagcaatata
     1621 caatgcttta tcatcataaa gtagataagc agttgtttga acatcagcca attttaattc
     1681 agaacaatca taaaaatttt ctgcttttgc cgctttttgc caacatccct ctaattttcc
     1741 atcaattata ggacgaacat cagtccatat tgcctcaatc tgcctttctt ccccattaac
     1801 agaatataaa aaacaaagca ggaaaatact tataataaat ataatttttt ctctcctcat
     1861 tttatctcct cctcgtataa tatgtttgta atgcctttaa tgtttttaag taagtattaa
     1921 tacaaatgtt ctttgaaaat ttgactaaaa aagccgggtt tttaaccatc atatcttttg
     1981 tgagatagag atatggaggg aaatgcttgc tcctccttgc acataaagtg ctttcaaaag
     2041 agagcatccc tttctcttat tggttaaatg ctaacttcca caggctcgcc cgttgggcta
     2101 cgcccagcgg gggatccggc ggacacacag tgcggctctt ctttctatct tctgtcactt
     2161 gactgggaca ggtgcatcca gatagacaag aaactactta taataagtcg aggttgcagt
     2221 ttcccataag agtaaagaac caatgtatat ctcaaaatac atctcacaag ggaggtgatt
     2281 ctttatgttc aaaacacttt ttgtaggtat agatatcagc cttaaaactg ctacctgttg
     2341 tttcttaaat caagaggata aacatcacgg gaaacc
//
LOCUS       DAOOVW010000094         2365 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_24579_length_2365_cov_9.237229, whole genome shotgun sequence.
ACCESSION   DAOOVW010000094 DAOOVW010000000
VERSION     DAOOVW010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24579_length_2365_cov_9.237229"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="PLW95_08095"
     rRNA            complement(<1..622)
                     /locus_tag="PLW95_08095"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(668..2014)
                     /locus_tag="PLW95_08100"
     CDS             complement(668..2014)
                     /locus_tag="PLW95_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOV22616.1"
                     /translation="MKKNDYSLFKILNEKEIKEEFIKKLSIIFSNLCKILNVFLSKRT
                     LPTREISFTLMKEIEKIETFLDDYGASHNRTFFYFRELIASIRWLNIALFHSLHIFAR
                     IENYELQIKKNEKEKFIKDLETNTWFYFSCIKNLIKELKKEAGKLGIEVHNEKFSEEL
                     VLPKIQKKILPPDLNINSGTNGGNQIFNFLVKFLEESEKVNFLVCKKIYPEEVTEENL
                     EKFRSIFHQLESMYDTFIIKTEIEKKTKYLITLRGYIALILHLLEIGRALTHFYERHS
                     DEVRKYKTAIKITKIVDKEKIKEIVKNFILFYSLFFISKGKSLSYKIFKSLGTEAEEF
                     IIQTVVLLIPAHRIEDFHIRPIMPITQIANKYKFDTYLYFQRKKYNLKNAIEVAIAIP
                     DIRDFLVNKDVELVVQGPEKAVKEICEFLKEKCGAQIQPECETFPLSLKISLDTSL"
BASE COUNT          669 a          451 c          354 g          891 t
ORIGIN      
        1 actgcttgta ggcatacggt ttcaggttct atttcactcc ccttccgggg ttcttttcac
       61 ctttccctcg tggtacttgt ccactatcgg tcgagtccgt gtatttagtc ttggaaggtg
      121 gtcctcccag attcctacgg agtttcccgg tttctccgta gtactcagga attcatctca
      181 ggtactttca aagttttgtc tacgggactt tcaccctcta tggttaacct ttccatgtta
      241 ttcaactacc ttgaaagatt tttatacctg ataggaaggt agaccttcct atgatgaacc
      301 ctacaacccc gaataacgaa cgcgtctacg ctattaccgt tattcggttt ggactattct
      361 ccgttcgctc gccgctactt agagaatcgc ttttgctttc ttttccttgg ggtactgaga
      421 tggttcactt cccccagttc gctttctgct gctatttata tttacagcag aatatctgcc
      481 ctttcaggca gatgggttgc cccattcggg aatccccgga tcaaaggttg cttgcaccta
      541 cccggggctt atcgcagttt gccacgccct tcatcgccag gactcgccaa ggcatcctcc
      601 gtatgccctt tgtagcttga ccataaattt aaattttcaa agaacattta tgtatattat
      661 acaaacttta taatgaagtg tcaagagata tttttaaaga taatggaaat gtttcacatt
      721 ctggttgaat ctgggctcca catttttctt ttaagaattc acatatttct ttaactgctt
      781 tttccggacc ttgaactaca agttcaacat ctttatttac taaaaaatcc cttatatctg
      841 gtatagcaat tgcaacttca attgcatttt ttaaattata ttttttcctt tgaaaataaa
      901 gatatgtatc aaatttatat ttattggcta tctgtgttat tggcattatg ggccttatat
      961 ggaaatcttc aatcctatgt gctggaatta acagaacaac cgtctgaatt ataaattcct
     1021 ctgcttctgt tcctaatgat ttgaaaattt tataagagag tgatttccct tttgaaataa
     1081 aaaataatga gtaaaaaaga atgaagtttt taacaatttc tttaattttc tctttatcaa
     1141 caattttagt gatttttatt gctgttttat attttctaac ttcatcacta tgtctttcat
     1201 aaaaatgtgt aagtgccctt cctatttcaa ggagatgtaa aattaaagca atataacctc
     1261 taagtgttat taaatatttt gtcttttttt caatttctgt ttttataata aaagtatcat
     1321 acattgattc taactgatga aatattgacc tgaatttttc aagattttct tctgttacct
     1381 cttctggata tattttttta cagactaaaa agttaacttt ttcactttct tcaagaaatt
     1441 taacaaggaa attaaaaatt tgatttcctc catttgttcc tgagttaata tttaaatcag
     1501 gtggaagaat tttcttttga atctttggca atacaagttc ctcagaaaat ttctcattat
     1561 gtacttctat tcctaatttt cctgcctctt tttttaattc ttttatcagg ttcttaatgc
     1621 atgaaaagta aaaccatgta tttgtttcca agtccttaat aaatttttcc ttctcatttt
     1681 ttttaatttg taattcataa ttttcaatac gagcaaatat atgaagagag tgaaaaagag
     1741 caatgtttaa ccatctaatt gaagcaatta attctctaaa ataaaaaaag gttctattgt
     1801 gagaagcgcc ataatcatca aggaatgttt caattttctc aatttctttc atcaatgtaa
     1861 aacttatttc tcttgttgga agagtccttt ttgacaaaaa aacatttaga attttacaga
     1921 gatttgagaa aataatagaa agttttttaa taaactcttc ttttatttct ttttcattta
     1981 atattttaaa aagagaataa tcattttttt tcattttttc cctcgtgcgt agtccgaagg
     2041 atccatagcc aaaaacgagg accctacgga cgagcctttc ccaacgatac ccacaatacg
     2101 aactgcttta ttagataacc ataaatatgg gaaaaagttt tttcccatac ccttttggtt
     2161 gcctggggta tatttttttt caatgttttt ttgcttactc ttttgtataa ctttttttgt
     2221 ggagatagcc ggagtcgaac cctcggacag aaccctacgg gcaagccggc aactcgctca
     2281 cgctcttggg acaggggtgc ttcccctttc ccaacgatac ccccaatacg aactgcttta
     2341 ttagataatt ataaatatgg gaaaa
//
LOCUS       DAOOVW010000095         2364 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_24597_length_2364_cov_9.173235, whole genome shotgun sequence.
ACCESSION   DAOOVW010000095 DAOOVW010000000
VERSION     DAOOVW010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24597_length_2364_cov_9.173235"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1393
                     /gene="mnmE"
                     /locus_tag="PLW95_08105"
     CDS             <1..1393
                     /gene="mnmE"
                     /locus_tag="PLW95_08105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOV22617.1"
                     /translation="MKKDTICAISTPLGVNGIGIVRLSGPDTYKIIEEIFVAGKNLKQ
                     GKKIKIKDIPSHTIHYGYIVESRRRIDEVLLTIMKSPKTYTKEDIAEINCHGGIIPLK
                     EVLSVCIKKGARLASPGEFTMRAFLNGRIDLTQAESVLEIITSQTEKSLEISLLKLEG
                     SFSEKINNIKEKIVRYLSQFEYLIDFPEEEDIKAIERVGEKEIDVLIKEIEKIISSSK
                     RGKILFEGVKVAIIGRTNVGKSSLLNKLIEEERAIVSEIPGTTRDIIQEVINIKGIPV
                     KIIDTAGIRKTKGKIEKMGVEKAVEWMKKAEINILILDGSQQLKKDDKKLFEKIKGNN
                     YIIAINKVDLPLKIEIEKIKKIFSDEKIVFISAKEGKGIDELKEKIFKIVEKGEKVSE
                     GEIYLNLRQEELIRKLKKALIGCLNSIKKNLPLEISTEYLRQAIREIDYLTGNRYNDE
                     VLENIFSNFCIGK"
     gene            1631..>2364
                     /locus_tag="PLW95_08110"
     CDS             1631..>2364
                     /locus_tag="PLW95_08110"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOV22618.1"
                     /translation="MNKIFKMGRFYKIIPVVLLFIFSGCATYYNPVTKKVEYTFYSEK
                     DEIDMGVAMDKKLQKELKIIPTPVKVEEIGQKIANACDRPNLKYTFRVVEGEDINAFS
                     IPGPFVYIYTGLLKNAEDDEIACVIGHEIGHICARDGVHRLQTQLLYSIPAAVLLSNA
                     RSEAIQKSIDTIFNLTMLKYSREEELRADALGVIYAYRGGYKPEGMVTFFKKLQEIEK
                     KNPKIIPAFLSDHPDIEERIKNVQAT"
BASE COUNT          956 a          276 c          441 g          691 t
ORIGIN      
        1 aatgaaaaaa gatacaattt gtgcgatttc aactcctctt ggagtaaatg gtatagggat
       61 tgtaagatta agtggtccag atacatataa aattattgaa gaaatttttg ttgccgggaa
      121 gaatttaaag cagggtaaaa aaataaaaat aaaggatatt ccatctcata caattcatta
      181 tggttatatt gttgaaagca gaagaagaat tgatgaggtc ttactaacca ttatgaaatc
      241 tccaaaaaca tatacaaaag aagatatcgc agaaattaat tgtcacggtg gaattatccc
      301 attaaaagag gttttaagtg tatgtattaa aaaaggagca cgacttgcca gccctggaga
      361 atttacaatg agagcatttc tcaatggtag aattgacctt acacaggcag aaagtgttct
      421 tgaaataata accagtcaga cagaaaaatc acttgaaatc tctcttttga aattagaagg
      481 cagtttttca gaaaaaataa ataatataaa agaaaaaatt gtgaggtact tatcacaatt
      541 tgagtacctg attgattttc ctgaggaaga agatataaaa gcaattgaaa gagttggtga
      601 aaaagaaatt gatgtcttaa taaaagaaat tgaaaaaatt atttcatctt ctaaaagggg
      661 gaaaatttta tttgaaggag ttaaagtagc aataattggg agaacaaatg taggcaaatc
      721 atcactcctt aataaattga ttgaagaaga aagagcaatt gtaagtgaaa ttcccggtac
      781 aacaagagat ataattcagg aagttataaa tataaaaggg attcctgtaa aaataattga
      841 tacagcagga ataagaaaaa caaaagggaa aattgaaaaa atgggagttg aaaaagcagt
      901 tgaatggatg aagaaagcag aaataaatat acttatttta gacggaagtc aacaattgaa
      961 aaaagatgat aaaaaattgt ttgaaaaaat caaagggaat aattatatca tagcaattaa
     1021 caaagttgac ctgcccttaa aaatagaaat tgaaaaaatt aaaaaaattt tttctgatga
     1081 aaaaattgta tttatttcag caaaagaagg taaaggtatt gatgaattaa aagagaaaat
     1141 ctttaaaatt gttgagaagg gagaaaaagt aagtgaaggt gaaatttatc tcaatttaag
     1201 gcaggaagaa cttataagaa aattgaaaaa agcacttatt ggttgtctta actcaattaa
     1261 aaagaattta ccgttagaaa tatcaacaga atatttgaga caggcaattc gtgaaattga
     1321 ctatctaact ggaaataggt ataatgatga agtacttgaa aatatatttt caaatttttg
     1381 tattggcaaa tagcaaacac tcatacttgg tagttactgt ttttttaggc ggtagcagtt
     1441 gtgctatctg ggaaacgatg acgcaccaac tctgctttgc taccctttgc ttctttagtc
     1501 ggtttatcaa agggggaact tatgggtagg ggatatcttt gggagagagg ctcgtcgata
     1561 aactatgccc gagtggatgg attttacttt gtgcctaaat ataacataac ttctctaaat
     1621 acttaaaaaa atgaataaaa tttttaaaat gggcagattt tataaaatta taccagttgt
     1681 tcttcttttc attttttctg gatgtgctac ttattacaat cctgttacaa aaaaagttga
     1741 atatacattt tattctgaaa aagacgagat tgatatggga gttgctatgg ataaaaaact
     1801 ccagaaagaa cttaaaatca ttccaactcc tgtcaaagtt gaagaaatag gacaaaaaat
     1861 tgcaaatgcc tgtgatagac cgaatttgaa atatactttc agagtagttg aaggagaaga
     1921 tataaatgct ttttcaattc caggaccttt tgtttatatt tatacagggc tattaaaaaa
     1981 tgcagaagat gatgaaattg catgtgttat cggacatgaa ataggacata tttgtgcaag
     2041 agatggagtt catagattac agacacaact tctttattca attccagcag cagttcttct
     2101 tagtaatgca aggtcagaag caatacaaaa atcaattgat acaattttca atttaacaat
     2161 gcttaaatat agtagagaag aagaattaag agcagatgct ttgggtgtaa tttatgctta
     2221 tagaggtgga tataaaccag agggaatggt aacatttttc aaaaaacttc aagaaattga
     2281 gaagaaaaat cccaaaataa ttcctgcttt tttaagcgac catccagata tagaagagag
     2341 aataaaaaat gttcaggcaa caat
//
LOCUS       DAOOVW010000096         2242 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_26560_length_2242_cov_11.135345, whole genome shotgun
            sequence.
ACCESSION   DAOOVW010000096 DAOOVW010000000
VERSION     DAOOVW010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26560_length_2242_cov_11.135345"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..>2242)
                     /locus_tag="PLW95_08115"
     CDS             complement(33..>2242)
                     /locus_tag="PLW95_08115"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOV22619.1"
                     /translation="IVRVRFSPDGVFPQSPLIRYGFVKDDFKPIKVKIEEKDKEIIAQ
                     TKRIKVVIEKSPLKIKYIDEKGVVIKMDNLEIIDKEYKVTFGISDKEGFFGFGDQTRD
                     RIQHYGTKPIMWIENVKRYIPIPFFMSSRGYGMFFNTTWRSKFDIGVTHKDKLSFLST
                     GGILDYYFIYGPDFKKILGLYTDITGKPYLPPLWSFGLWFICRTQANDFEVVSDALNF
                     RDRKIPCDVLGLEPGWMSENYDLSINKKWHPERFPIPSYAMKGPHTFISAIKRLGFKL
                     ELWLCNDYDLSIEEERILGNDISSPKISREFAEDDKEKDKHFTEPIIMDKLTIPDQPW
                     FEHLKKFIDQGVDFFKQDGAFQVCEHPDRKWANGMTDEEMHNLYPLLYSKQMYLGFKN
                     YTGRRPCGFTPAGWAGLQKFTGTWTGDTGGGPKTLVACLNLCLSGHSLNTCDMEVFTK
                     EGIHYGFLLPWAQVNSWNYWKHPWLLGKELEEVFKFYAELRYKLIPYIYTYAYLSHKT
                     GIPMMRPLILEFPDDKNTWNILTQYMFGEELMVSAFTDKIYLPAGKWIDYWTGKEYTG
                     PTTIEYKIPENRGGGLFVKSGSIIPFALEMDYIGEKKLDEITIEIFPEKNGKFLLYED
                     DGVSFNYEKGEFCITEFKYEMNNKNIRLMIGERKGKYKGIPETRKYIIIVHNIVKEPI
                     EVQCGEEEKLLTKTKDMSDFKNMKESFIYNAETKTLYLKINEDKKDTFLNVIF"
BASE COUNT          680 a          439 c          244 g          879 t
ORIGIN      
        1 atttttcatt atttattttc accttataaa gatcaaaaaa taacatttaa gaaagtatct
       61 tttttgtctt catttatttt tagataaagg gttttagttt ctgcattata tataaaagat
      121 tctttcatat ttttaaaatc actcatatct tttgttttag tcaataattt ttcttcctct
      181 ccacattgca cttcaattgg ttctttaaca atgttatgga ctataataat atattttctt
      241 gtttcaggta ttcctttata tttccctttt ctttccccta tcatcaatct aatgtttttg
      301 ttattcattt catacttaaa ttctgttata caaaattctc ctttctcata attaaaactc
      361 actccgtcgt cttcgtacaa tagaaatttc ccattttttt ctggaaatat ctcaatcgtt
      421 atctcatcaa gttttttctc tccaatatag tccatttcaa gagcaaatgg aattatactg
      481 cctgatttta caaataaccc tccaccccta ttttcaggta ttttatattc aatagttgtt
      541 ggtccagtat attctttccc agtccagtaa tctatccatt taccagcagg taaatatatt
      601 ttatctgtaa aagcactgac catcaattcc tctccaaaca tatattgagt aagaatattc
      661 catgtatttt tatcatcagg aaattctaaa attaaaggtc tcatcattgg tattcctgtt
      721 ttatgagata agtaagcata tgtgtaaata taagggatta atttatagcg aagttctgca
      781 taaaatttaa atacttcttc taactctttc ccaagtaacc atggatgttt ccaataattc
      841 caactattaa cttgtgccca tggtagcaaa aaaccataat gtattccctc ttttgtaaac
      901 acttccatat cacatgtgtt aagcgaatgc cctgataaac ataaatttaa acatgcaaca
      961 agtgtttttg gccctcctcc tgtatctcct gtccatgttc cagtaaactt ttgtaatcct
     1021 gcccacccag ccggtgtaaa accacatggc cttcttccag tataattctt aaatcctaaa
     1081 tacatctgtt ttgaatataa taatgggtaa agattatgca tttcttcatc tgtcatacca
     1141 tttgcccatt tcctatcagg atgttcacat acctgaaatg ctccatcttg tttaaaaaaa
     1201 tcaactccct ggtctataaa cttttttaaa tgttcaaacc atggctggtc aggaatagtc
     1261 aatttatcca taataattgg ttctgtaaag tgtttatctt tttctttgtc atcttcagca
     1321 aattctcttg aaatttttgg actactgata tcgtttccta agatcctttc ttcttcaata
     1381 cttaaatcat aatcattaca taaccataat tctaatttaa accccaatct ttttattgct
     1441 gatataaatg tatgtggtcc tttcattgca taagaaggaa tagggaacct ttctggatgc
     1501 cattttttat ttatagacag gtcataattt tcactcatcc atcctggttc taaaccaagc
     1561 acatcgcacg gtatttttct atctctgaaa tttaatgcat cgcttacaac ttcaaaatca
     1621 tttgcttgtg tcctgcatat aaaccataat ccaaatgacc ataaaggagg caaatatggt
     1681 ttacctgtta tatcagtgta tagtcccaat atcttcttaa agtcaggtcc ataaataaaa
     1741 taataatcta aaattccacc tgttgataaa aaagaaagtt tatctttatg tgttacccct
     1801 atatcaaatt tgcttcgcca tgttgtatta aaaaacattc catatcctct actgctcata
     1861 aaaaacggta tgggtatata tcttttaaca ttttctatcc acattatagg tttagtacca
     1921 taatgctgta ttctatctct tgtctggtcc ccaaatccga agaaaccctc tttatcagat
     1981 attccaaagg taaccttata ttctttatct ataatttcta aattatccat ctttataact
     2041 accccttttt catcaatata cttaattttt aaaggacttt tttcaattac cactttaatt
     2101 ctttttgttt gtgcaataat ttctttatct ttttcttcta tttttacctt tattggctta
     2161 aaatcatctt tcacaaatcc atatcttata agtggtgact gtggaaaaac tccatcagga
     2221 gaaaatctta ctcttactat at
//
LOCUS       DAOOVW010000097         1991 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_31519_length_1991_cov_9.603822, whole genome shotgun sequence.
ACCESSION   DAOOVW010000097 DAOOVW010000000
VERSION     DAOOVW010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31519_length_1991_cov_9.603822"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            479..778
                     /locus_tag="PLW95_08120"
     CDS             479..778
                     /locus_tag="PLW95_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22620.1"
                     /translation="MSTKFFSKEAPILGISSRTTKDKKGGRQENIWGRSGGKEEGIFA
                     KTLDTTDIFTGWTETICVENKSPEKVFKENQLPETNFSDAVKTMELVENIYKNTI"
     gene            complement(771..1664)
                     /locus_tag="PLW95_08125"
     CDS             complement(771..1664)
                     /locus_tag="PLW95_08125"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOV22621.1"
                     /translation="MKIATISMACEHRKIEKIQDNLEYIEKTFEEISNIKPDLVCLPE
                     IFYYAGVEKRGDFYSSEIKVFLKNLAKKYKTCIAGSIYDEREKGVFNTCVFINREGEI
                     IGKYDKIHPTESEIEDGIIPGSKEQKVVETEFGYLAAQICFDANWFDEWKKFSDMGAK
                     LIVFSSSYPGGKVLNSISLLNSIYIVASIREELKAGIIDNTGRWIVKTNRFSYWVWCD
                     INLERTVFHWDFQGDRLKGILKEYRDKIKIETFGDEALFTIEPISKDVKIDEIIKKYE
                     LVTYKEYIKRATKIQDKTRTK"
     gene            complement(1661..1987)
                     /gene="trxA"
                     /locus_tag="PLW95_08130"
     CDS             complement(1661..1987)
                     /gene="trxA"
                     /locus_tag="PLW95_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013553626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOV22622.1"
                     /translation="MEEKIIELTNDNFDNFLKEKEFVIVDFWAPWCMPCRMVSPIIEN
                     LSKKYEGKISFGKLNVDQQQDIAVKFGIMSIPTIIIFQNGKEVDRIIGAMPEEMFEER
                     IKGVLK"
BASE COUNT          704 a          299 c          276 g          712 t
ORIGIN      
        1 agaaaaatat agtaagaaag gatatttttt acctggtgag atagaaaaat cttttgcaga
       61 aataggactt aataaccttt ttcagtagta attattcttt gtgcaaattg aatagaagta
      121 aattttatta aaatcaaaaa attagaaaga ataagaaaat aaaaacaaat aaaaagatat
      181 aggtaatttt acaggtagta tcaaaaaata gatgaaagag aaaaaattgt tcattgaaaa
      241 ttttggggat agaaaaaagc acgggaaggg tgaaaaaaga ggactaggaa gtaaataggg
      301 cataaatgag caataaggca gggactatat caagaaatag gtaataaggt tatttaaaaa
      361 agattcaggg caatttagag gcaatatgtc cgtagaatcc ctcgggcgta gcccagaggg
      421 atcctttaga ttggtaagaa accatttttt gtattttctc ttttcccttg atattcctat
      481 gtctacaaag tttttttcaa aagaagcgcc gatcttgggt attagtagca gaacaacaaa
      541 agataaaaag ggaggaaggc aggaaaatat atggggaaga agtggcggga aagaagaagg
      601 gatatttgcc aaaacattag atacaacaga tatttttact gggtggacag agacaatatg
      661 tgttgagaac aaatcaccgg aaaaagtatt taaagaaaac caattaccag aaacaaattt
      721 ttctgatgcg gttaaaacaa tggaacttgt agaaaatatt tataaaaaca ctatttagtt
      781 cttgttttat cctgaatttt tgttgctctt tttatgtatt ctttataagt tacaagttca
      841 tattttttaa ttatttcatc aatttttaca tcttttgaaa ttggttcaat agtaaataat
      901 gcttcatcac caaatgtttc tatttttatt ttatctctat attctttcaa aatacctttt
      961 aatctatctc cttgaaaatc ccagtgaaac acagttcttt ctaaattaat atcacaccag
     1021 acccagtaag aaaatctgtt tgtttttaca atccatcttc cagtgttatc tataattcct
     1081 gcttttaatt cttctcgtat agaggcaaca atatatatac tatttaataa agagattgaa
     1141 tttaaaactt ttccaccagg ataagaggaa gaaaatacaa ttagttttgc acccatatct
     1201 gaaaattttt tccattcatc aaaccaattt gcatcaaaac atatttgtgc tgctaaatat
     1261 ccaaattcag tttcaactac cttttgttct ttacttccag gaattattcc atcttcaatt
     1321 tctgattctg ttggatgaat tttgtcatat tttcctataa tttctccttc tctatttata
     1381 aaaacacagg tattaaaaac acctttttct cgttcatcat aaatagaacc agctatgcat
     1441 gttttatatt ttttagccaa attttttaaa aatactttaa tttcagaact ataaaagtca
     1501 cccctttttt ctacccctgc ataatagaaa atttcaggta gacaaacaag gtctggtttt
     1561 atatttgata tttcttcaaa tgttttttca atatattcaa gattatcctg aattttttct
     1621 atttttctat gttcacatgc catagagata gttgcaattt tcatttgagt acccccttta
     1681 ttctttcttc aaacatttcc tctggcattg ctccaataat tctatccact tctttaccat
     1741 tttgaaaaat tataattgtt ggaatagaca taattccaaa tttaactgca atatcctgtt
     1801 gttggtctac atttaattta ccaaaagata ttttcccttc atattttttt gagagatttt
     1861 caattattgg agataccata cgacagggca tgcaccatgg agcccaaaaa tcaacaatta
     1921 caaattcctt ttcctttaaa aaattatcaa aattatcatt tgttaattca attatttttt
     1981 cttccatttt t
//
LOCUS       DAOOVW010000098         1891 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_34074_length_1891_cov_7.694444, whole genome shotgun sequence.
ACCESSION   DAOOVW010000098 DAOOVW010000000
VERSION     DAOOVW010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34074_length_1891_cov_7.694444"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1483
                     /locus_tag="PLW95_08135"
     CDS             <1..1483
                     /locus_tag="PLW95_08135"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOV22623.1"
                     /translation="TKKKIVGLVVGIVLVLSFIVVAGVKNFKEVPKLPNPSTFVSVSP
                     EVMSLQNAFVSVSKAVRPSVVQITTEKTITYRYWDPFGDIEDFFNFPFDNFFSIPKRN
                     QQPKTFKKKQEGLGSGFIVDPDGYILTNNHVIEGVDRVLVKLFDDENKYPAKIIGTDP
                     KTDLALIKIDVKRALPSVVLGDSDTIQVGEWAIAIGNPMGLTETVTVGVISATGRSGF
                     GITQYEDFIQTDAAINPGNSGGPLVNIKGEVIGINTFIITPYAAQNIGFAIPINIAKS
                     VFKQLKEKGKVVRGYLGIVLQPLTDDLSKSFGLNNTKGAVVAQVMSNSPAEKGGLKVG
                     DVIVEFDGQKIEDVKDLQMKVANTPVGKKVKVGIFRDGKAITLEVQVGEMPEEEKVAK
                     TEEEQSGLWFGMKVSEITDQIANKYGLENKEGVIVVYVEQGSVADESGIIPGSVIKKI
                     DKYTIKNYADFLEAKKNVNSKQYISVWIVINGQGRFVVLKKSE"
     gene            1476..>1891
                     /locus_tag="PLW95_08140"
     CDS             1476..>1891
                     /locus_tag="PLW95_08140"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOV22624.1"
                     /translation="MNKDEKIVFYKVLKLLSKKNYSENELRNKLNSVDNDIVNRIIEK
                     LKEKGLIDDKKYSHDIVERYLERGKGFYYIINILKRKGFNEEIINQIRQNFDFEKEYR
                     VAQALIKKRKNKGKNNLFLYLKGRGFSSETINKIIE"
BASE COUNT          754 a          192 c          374 g          571 t
ORIGIN      
        1 aacgaaaaaa aagatagttg ggttagttgt aggaatagta ttagttttat ctttcattgt
       61 tgttgctgga gttaaaaatt ttaaagaagt acctaaatta cctaatccca gtacttttgt
      121 ttctgtttca ccagaagtaa tgtctttaca aaatgctttt gttagtgttt caaaagctgt
      181 aagaccaagt gttgttcaaa ttaccacaga aaaaacaata acttatagat attgggaccc
      241 atttggtgat atagaggatt tttttaattt cccttttgat aattttttta gtattcctaa
      301 gagaaaccag caaccaaaaa catttaaaaa gaaacaggaa ggtttaggtt ctggatttat
      361 tgttgaccct gatggatata ttttaacaaa taaccatgta attgaaggag tagatagagt
      421 tcttgttaaa ctatttgatg atgaaaataa atatccagca aaaattatag gaacagaccc
      481 aaagacagac cttgcactta taaaaattga tgttaaaagg gcactaccat ctgttgtttt
      541 aggtgattct gatacaatcc aggttggtga atgggcaatt gcaataggaa atcctatggg
      601 cttgactgaa acagtgacag tgggcgttat aagtgcaaca ggtagaagtg gttttggaat
      661 aacacaatat gaggatttta tacagacaga tgcagcaatt aatcctggaa attctggtgg
      721 accactggta aatataaaag gagaagttat agggattaat acatttatta ttactcctta
      781 tgctgcccaa aatattggat ttgctattcc tataaatata gcaaaaagtg tttttaaaca
      841 acttaaagaa aagggtaaag ttgtaagagg atatttagga attgttcttc aaccactcac
      901 tgatgacctt tcaaaatcat ttggactgaa taatacaaaa ggagcagttg ttgcacaggt
      961 tatgagtaat tcacctgctg aaaaaggtgg tttaaaagta ggagatgtaa tagttgaatt
     1021 tgatgggcaa aaaattgaag atgttaagga tttgcaaatg aaagtagcaa atacaccggt
     1081 tggaaagaaa gttaaagtgg ggatttttag agatggaaag gcaataacat tggaagttca
     1141 agttggagaa atgcctgaag aagagaaagt tgcaaagaca gaagaagaac aatctggttt
     1201 atggtttgga atgaaagtaa gcgagataac agaccagata gcaaataaat atggacttga
     1261 aaataaagaa ggagttattg ttgtttatgt tgaacagggg tctgttgctg atgaaagtgg
     1321 gataattcct ggaagtgtta taaaaaaaat tgataaatat acaataaaaa attatgctga
     1381 ctttttagaa gcaaagaaaa atgtaaattc aaaacaatat ataagcgtct ggatagtaat
     1441 aaatgggcag ggcaggtttg ttgttcttaa aaaaagtgaa taaagacgaa aaaattgtct
     1501 tttataaagt attaaaactt ctttcaaaaa aaaattattc tgaaaatgaa ttaagaaata
     1561 agttaaattc agttgataat gatatagtta acagaataat tgaaaaatta aaagaaaagg
     1621 gtcttattga tgataaaaag tattcccacg atattgttga aagatattta gaaagaggaa
     1681 aaggatttta ttatattata aacatattaa aaagaaaagg atttaatgaa gagataataa
     1741 atcaaattag acaaaacttt gattttgaaa aagaatatag agttgctcag gcacttataa
     1801 aaaaaaggaa aaataagggg aaaaataatt tatttttata tttgaaagga agaggttttt
     1861 cttctgaaac aataaataag ataattgaaa g
//
LOCUS       DAOOVW010000099         1832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_35716_length_1832_cov_8.348340, whole genome shotgun sequence.
ACCESSION   DAOOVW010000099 DAOOVW010000000
VERSION     DAOOVW010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35716_length_1832_cov_8.348340"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..1766
                     /locus_tag="PLW95_08145"
     CDS             93..1766
                     /locus_tag="PLW95_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOV22625.1"
                     /translation="MERKELGQILIEKGIITPLQLEEALQEQKKTDKFLGEILVEKKF
                     TTKEDIVHALTEQKKADTVVLSRFRGLKKEDIKIIPENIARKFTLIGIAKENETLIVA
                     MKDPLDIVAMDTVRRITGMKIKVVKAEEKEILDLIERYYNEEKDIPEMFQELENSREQ
                     EEVVDENQLRIAAEDAPIIRFVNSLFLNAIEKRATDIHLEPGEIDINVRFRIDGILHS
                     SFQSPPKYAYPGIVTRMKILSNLDIGERRLPQDGRFKIKVGIREIDVRISTLPTIHGE
                     KVVMRLLDRENLVLDMEQLGFEAEELKKFKGSLMRPFGMIIVTGPTGSGKTTTLYSGL
                     TFINTSEKNIITIEDPVEYQLERINQVQIKPKIGLTFPVVLRHILRQDPDVIMIGEIR
                     DIETAQIAIQSSLTGHLVISTLHTNDTVSTISRLSYMGVENYLISDAVNLILSQRLIR
                     KICERCKEKDKEGKTTLEKIGIDVAKKEIYKGRGCIDCNYTGYYGRIAIYEVLEVNRK
                     IREMIIEKKSEDKIRDAAKENGMKTLREIGIKKVVDGITTVKEALSVTL"
BASE COUNT          767 a          205 c          330 g          530 t
ORIGIN      
        1 tttataaaat gtcaaaagga aaaatagaat aaaacctctg catattacaa ttggttgtgt
       61 aaatagatgt tttatggtaa aataatattg gtatggaaag aaaagaatta gggcagatac
      121 ttattgaaaa aggtattatt actcctttac aacttgaaga agcattacag gaacaaaaaa
      181 agacagataa atttttaggt gaaatacttg ttgaaaagaa gttcacaaca aaagaagata
      241 tagtacatgc tcttactgaa cagaaaaaag cagatactgt tgttctttcc agatttagag
      301 ggttaaaaaa agaagatata aaaataattc cagaaaatat tgcaagaaaa tttaccctta
      361 ttggaattgc aaaggaaaat gaaacactta ttgtagcaat gaaagaccct ttagatattg
      421 tagcaatgga tacagtaaga agaattactg gaatgaaaat aaaagttgta aaagcagaag
      481 aaaaggaaat ccttgactta attgaaaggt attataatga agaaaaagat attcctgaaa
      541 tgtttcaaga acttgaaaac agtagggaac aagaggaagt agtagatgaa aaccaattaa
      601 gaattgctgc ggaagatgct cctataataa gatttgtaaa ttcacttttt ttaaatgcaa
      661 ttgaaaaaag agcaactgat attcatttag agccaggaga aatagatatt aatgttagat
      721 ttagaataga tggtattctt catagtagtt ttcagtcacc tcctaaatat gcttaccctg
      781 ggattgttac acgaatgaaa attctatcaa accttgatat tggtgaaaga agattacctc
      841 aggatggaag atttaaaatt aaagtaggca taagagaaat agatgttaga atttcaactc
      901 ttcctacaat acatggagaa aaagtggtaa tgcgactttt agatagagaa aatcttgttt
      961 tagatatgga acaattaggg tttgaggcag aagaattaaa aaaatttaaa ggctccctta
     1021 tgagaccgtt tggaatgata atagtaacag gtccaacagg aagtggaaaa acaactactt
     1081 tatattctgg cttaactttt ataaatacat ctgaaaaaaa tataataacg attgaagacc
     1141 ctgttgaata tcaattagaa aggataaatc aagttcagat aaaaccaaaa attgggctta
     1201 cttttccagt tgttttgcga catattttaa gacaagaccc tgatgttata atgattggag
     1261 aaattagaga tattgaaacc gcacaaattg ctattcaatc atcacttaca ggacatcttg
     1321 ttatttctac ccttcataca aatgatactg tttctactat ttcaagatta tcttatatgg
     1381 gggttgaaaa ttatcttata tctgatgctg taaatttaat tctttctcaa agattaatta
     1441 gaaaaatatg tgaaagatgc aaagaaaaag ataaagaagg aaaaactact cttgaaaaaa
     1501 ttggaattga tgtcgctaaa aaagaaattt ataaaggtag aggttgtatt gattgtaatt
     1561 atactgggta ttatggtaga attgctattt atgaagtatt ggaagtaaat agaaaaataa
     1621 gagaaatgat aattgaaaag aaaagcgaag ataaaataag agatgcagca aaagaaaacg
     1681 gaatgaaaac attaagagaa attggaatta aaaaagttgt tgatggaatt acaactgtaa
     1741 aagaagcgct aagtgtaaca ctgtaaataa aaagtacaaa agcatctaca ttttcccctt
     1801 tttaaaaaaa gattctcccc tttgacaaag gg
//
LOCUS       DAOOVW010000100         1829 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_35803_length_1829_cov_7.428410, whole genome shotgun sequence.
ACCESSION   DAOOVW010000100 DAOOVW010000000
VERSION     DAOOVW010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35803_length_1829_cov_7.428410"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            278..616
                     /gene="rsfS"
                     /locus_tag="PLW95_08150"
     CDS             278..616
                     /gene="rsfS"
                     /locus_tag="PLW95_08150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOV22626.1"
                     /translation="MRRCSIKRIIKKIIKSIEDKKGTDLKVLDVSKLTWICDYFIIAS
                     GETTIQTKVIAENILEKIKERVISVEGLEDGKWILIDYGEIIIHIFLTETRNYYNLDK
                     LWGDAEIIKV"
     gene            complement(606..1052)
                     /locus_tag="PLW95_08155"
     CDS             complement(606..1052)
                     /locus_tag="PLW95_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22627.1"
                     /translation="MEKKKYKRRHYLINRSIQFTYAGIAIYLLLIAIILVGSFTYYIT
                     LNTILNQLQLENQLIDAYKIVSSINSIILKRIGILFFALILLVFILEILFLHRIAGPL
                     YRIEKTLNDIADGKKVEFIKLRKKDFLKSLAISVNKVIEKINSFKL"
     gene            complement(1062..1787)
                     /locus_tag="PLW95_08160"
     CDS             complement(1062..1787)
                     /locus_tag="PLW95_08160"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HOV22628.1"
                     /translation="MKKGFTLIEILVAVLIIFIICLISFRLISTVIQKSKVASAKVHI
                     SQLALLLEQVKNDTGYYPVFLSDMTSNTPPKMLEKGWFGPYINEIPLDPWKHPYFYKI
                     PPTTLFASPSIPRVSGTPDTFTAFFEGIQGKAILHIENYGLTSCEITLNGVKVLNENE
                     LKNKPIPQIIEKEVDILEGNNIFTRARSTPGDSLIFSISSYNIPTGKYFILGSYGKDG
                     KEGGKGFNKDIVWYSDKYPNFQQ"
BASE COUNT          664 a          240 c          264 g          661 t
ORIGIN      
        1 aaaactctaa acccttctgt gtaagacaat ccatcccctc gggtgtagcc cagagaaatt
       61 ctgtggacta ctataggcga gcccactttc catctccctt tcatctaagt ttttaacact
      121 accactgaaa tataattttt ttaaaatttt cttttattta atttatcatt tttcttttct
      181 acctgtcaat ttattaaaaa aaaattattt tatggtaata ttttatatat ataaaatgtg
      241 ttatataaac atttttttat tagaatggta aaatatagtg cggaggtgta gtataaaaag
      301 aataataaaa aaaataatta agtcaataga ggataaaaaa gggactgact taaaagttct
      361 tgatgtaagt aaattaacat ggatatgtga ttattttatt attgcttctg gtgaaaccac
      421 aattcaaaca aaagttattg cagaaaatat tcttgaaaaa ataaaagaaa gggttatttc
      481 tgttgaagga cttgaagatg gaaagtggat attaattgat tatggagaaa taataattca
      541 tatttttctg accgaaacaa gaaattatta taatcttgat aaactatggg gagatgcaga
      601 aataattaaa gtttaaatga atttattttt tcaattactt tattaacaga aatagccagt
      661 gattttaaaa agtccttttt tcttaattta ataaattcaa cttttttccc atctgcgata
      721 tcgtttagtg ttttttctat tctatataaa gggcctgcaa ttctatgaag aaaaagtatt
      781 tcaagaataa aaacaagaag gataagggca aaaaataaaa taccaatgcg tttgagaata
      841 atagaattga ttgaagaaac aattttgtat gcatcaataa gttgattctc caattgtaat
      901 tgattcaaaa tagtatttag ggttatataa tatgtaaaac ttccaacaag tataattgca
      961 ataagtaaaa gatatatagc aatacctgca taagtaaatt gtattgacct atttattaaa
     1021 taatgtctcc ttttgtactt ctttttttcc atctttcacc tctattgttg aaaatttggg
     1081 tatttatctg aataccatac aatatcttta ttaaaaccct ttcccccttc tttgccatct
     1141 tttccataac ttccaaggat gaaatatttt ccagttggaa tattataaga cgaaattgaa
     1201 aaaattaagg aatcaccagg agtacttctt gccctcgtaa agatattatt cccttcaaga
     1261 atatctactt ctttttctat tatttgaggt attggtttat ttttaagttc attttcattt
     1321 aaaactttga cgccatttaa agttatttcg catgaagtta atccataatt ttctatatga
     1381 agaattgctt ttccctgaat accttcaaaa aaagcagtaa aagtatcagg agttccagat
     1441 actcttggaa tagaaggaga agcaaataaa gttgtcgggg ggattttata gaaatatgga
     1501 tgtttccatg ggtcaagagg tatttcattt atataaggtc caaaccatcc tttttctaac
     1561 atcttaggag gtgtgttaga agtcatatca gaaagaaata caggataata gccagtatca
     1621 tttttaacct gttctaaaag taaagcaagt tgtgagatgt gcacttttgc actggcaact
     1681 tttgattttt gtataacagt actaatgagg cgaaaagata tcaaacaaat aatgaaaatt
     1741 attaaaactg ctacaagaat ttctataaga gtaaatccct ttttcattat aataatttta
     1801 aattatgaat atattgtttg tcaagtatt
//
LOCUS       DAOOVW010000101         1645 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_42107_length_1645_cov_9.708176, whole genome shotgun sequence.
ACCESSION   DAOOVW010000101 DAOOVW010000000
VERSION     DAOOVW010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42107_length_1645_cov_9.708176"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PLW95_08165"
     CDS             <1..186
                     /locus_tag="PLW95_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22629.1"
                     /translation="YLLNDNIFLTIFNFRLSYIIIYFIIVPNIVSKIIEMIVKIYQRR
                     IIDIKLKIRTKDKTQEK"
     gene            195..266
                     /locus_tag="PLW95_08170"
     tRNA            195..266
                     /locus_tag="PLW95_08170"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:227..229,aa:Lys,seq:ctt)
     gene            complement(822..1589)
                     /locus_tag="PLW95_08175"
     CDS             complement(822..1589)
                     /locus_tag="PLW95_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV22630.1"
                     /translation="MKKIIVFVFGVFCFFSFAEINKVEVWNEISKKYQNVSEISGEIE
                     IKMDMMGKIMTMPMKFWAKEKKFKMETVYQEQGMEKPMEIVMLMDGEKLIQYQKSINT
                     VMKFDLTKFPEELKKDWEKQYNFKDNIPLDIEKISNNVDLEIKNKGGKEYYVLTINNL
                     QTLNIPGLQNINGKSQFFTKSVVWINRDNLLPESISYFADKETPGMEIVFKSLNLSPV
                     SDDIFKINIPDDAKILDMTDMMINMANTMKQQITNPQ"
BASE COUNT          545 a          294 c          241 g          565 t
ORIGIN      
        1 tatcttttaa acgacaacat attcttgacg atatttaatt ttaggttatc atatattatc
       61 atttatttta ttattgttcc caatattgtt tccaaaataa tagaaatgat agtaaaaata
      121 taccaaagaa ggataataga tataaaactg aaaataagaa caaaagacaa aacacaagaa
      181 aagtaatacc tcatgggccg ttagctcaat ggcagagcag ggcactctta atgccaaggt
      241 tgcaggttca agtcctgcac ggcctataaa aactggttaa acaaaaataa cttatctact
      301 ttaacaatcg gaaataacta cctgcaacca aaagggtatg ggaaaaaaca ttttcccata
      361 tttataatta tctaataaag cagttcgtat tgggggtatc gttgtaaata cttactcaac
      421 cacattaatt taaaagcaca aaagcccccc tcaccctcgc cctctcccct gtgggtagag
      481 ggtaataaga ggaaaaaagt tcttctcccc agcggggaga aaatagatgc agggataggg
      541 tgtttttctc tttatagaaa tggaaagaca gaaaagtagg gaagaaattt aatatggtta
      601 ataactcctg cgtctaaaaa gggtatggga aaaaactttt tcccatattt ataataatct
      661 aataaagtag ttcgtattgg gggtatcgtt ggaaaggctc gctcgtagag catacgcccg
      721 agggggatgc tcccctgtcc caagagcgga agcgagttgc aggcttccgc caaaaagagt
      781 ggcggacaag caatgtcctc cacggcctat gaaaacaatg tctattgggg atttgttatc
      841 tgttgtttca ttgtattagc catatttatc atcatatcag tcatatcaag aatttttgca
      901 tcatctggta tattgatttt aaaaatatca tcactgacag gtgataaatt caatgactta
      961 aaaactattt ccatcccagg agtttcttta tcggcaaaat aagaaatact ttctggtaat
     1021 aaattatccc tatttatcca aacaacactt tttgtaaaaa attgtgattt accatttata
     1081 ttttgtagac caggaatatt taatgtctgt aaattattaa ttgtcagaac ataatattct
     1141 tttccacctt tattttttat ctccaaatca acattattag aaattttttc aatatcaagg
     1201 ggaatattat ctttaaaatt atattgtttc tcccagtcct ttttcaattc ttccggaaat
     1261 tttgttaaat caaatttcat tactgtattg attgattttt gatattgaat caatttttct
     1321 ccatccatca acatcactat ttccatgggc ttttccatcc cctgttcttg atatactgtt
     1381 tccattttaa attttttctc ttttgcccag aatttcatag gcattgtcat tatttttccc
     1441 atcatatcca tttttatttc tatttcacca cttatctcag aaacattctg atactttttt
     1501 gaaatctcat tccaaacttc tactttattt atttcagcaa aagagaagaa acaaaaaacc
     1561 ccaaatacaa aaacaattat ttttttcata gtgcctcctt tttttcatta tggttctgcc
     1621 aaaattggct atatctttta taatg
//
LOCUS       DAOOVW010000102         1514 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2431
            NODE_47820_length_1514_cov_5.878684, whole genome shotgun sequence.
ACCESSION   DAOOVW010000102 DAOOVW010000000
VERSION     DAOOVW010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864506
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:18:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,636
            CDSs (total)                      :: 1,589
            Genes (coding)                    :: 1,582
            CDSs (with protein)               :: 1,582
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47820_length_1514_cov_5.878684"
                     /isolate="AMR_MDS_2431"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1503)
                     /locus_tag="PLW95_08180"
     CDS             complement(<1..1503)
                     /locus_tag="PLW95_08180"
                     /inference="COORDINATES: protein motif:HMM:NF026240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOV22631.1"
                     /translation="MSGSDFVEMFVGVGASICGDEMVLIKENNQVKLQPISKVVDKYY
                     RKKGEGYPVAVSNLETLGFYKGTTNFWGFKNNLDKFYFGGAKWSKIKSVYRHKVEEIY
                     EIHYLGGKIKTTGDHSVFVREKNYIVAKQVRDLKEGDILVNIPFKVRSSFIPNYGTTH
                     KIMSHKFEKCEIEKISLWENDKKIEENYQFIMENKGKIPQGQLAKRTGVSQTTISNWQ
                     MKKYSPASLSLVLNTSRRKIPKQIEITPSLLLLLGYYTAEGRTTPYYVQFIFGSHEKA
                     LHKDTINLMKDIFNVDPHLEYTSDNTLRITYHSKILSEFFEKTCGNGSHNKHIPEFLW
                     KLPKEYFLNYLKGFSKGDGYISKEGNLSMSTVSKQLALEISWLSSMHSIGVSCYKRKN
                     KEGRVIKNKPITCGSFWTIKISKTSSPFNEHNQSSFQFKKPIVKKIVKKHYDGFVYDF
                     CGCDNEGFFAGNKPILVHNSRVRDLFRQAKKMAPCIIFIDEIDAVGRQRFA"
BASE COUNT          471 a          259 c          198 g          586 t
ORIGIN      
        1 ggcaaaacgt tgtctaccta ctgcatctat ttcatcaata aaaataatac atggagccat
       61 tttttttgcc tgccgaaaga ggtcccgtac tctgctgtta tgaacaagta ttggtttgtt
      121 tccagcaaaa aatccttcat tatcacaacc acaaaaatca tatacaaaac catcataatg
      181 ttttttaaca atttttttaa caattggttt tttaaactga aaagaacttt ggttatgttc
      241 attaaaagga gatgatgttt ttgaaatttt tatagtccag aaacttccac aagttattgg
      301 tttattttta ataactctac cctctttatt ttttctctta tagcaagaaa ctccaatact
      361 atgcattgaa gaaagccatg aaatttccaa tgcaagttgt ttgctcacag tagacattga
      421 caaattacct tcttttgaaa tatatccatc ccctttacta aatcctttta agtaatttaa
      481 aaagtattcc ttgggtaatt tccataaaaa ttcagggata tgtttgttat gagaaccatt
      541 accacaagtt ttttcaaaaa actcacttaa tatctttgaa tgatatgtaa tccttaatgt
      601 gttatcagat gtatattcaa gatgtgggtc aacattaaaa atatccttca ttaggtttat
      661 agtgtcttta tgcagtgctt tttcatggga accaaaaata aattgaacat aataaggagt
      721 tgttctccct tctgctgtat aatatccaag tagcaataaa agagagggag taatttcaat
      781 ttgttttgga atttttctcc ttgaagtatt aagaactaat gaaagagaag caggggaata
      841 tttcttcatt tgccagttgc tgattgtagt ttgtgaaact ccagttcttt ttgcaagttg
      901 cccctggggt attttccctt tgttttccat aatgaattga taattttctt ctattttttt
      961 gtcattttcc cagagagata ttttctctat ctcacatttt tcaaatttat gagacatgat
     1021 tttatgtgtt gttccataat ttggaataaa actgcttctt actttgaaag gaatattgac
     1081 aagtatatct ccttctttta agtcccttac ttgttttgct acaatataat tcttttctct
     1141 aacaaataca ctatggtctc ctgttgtttt aatttttcca ccaagatagt gaatttcata
     1201 tatttcttct actttatgtc tataaacgct ttttattttt gaccatttgg caccaccaaa
     1261 gtaaaatttg tctaaattat ttttaaagcc ccagaaattg gttgttccct tataaaaacc
     1321 aagtgtttca agatttgaaa ctgctactgg ataaccctct cctttttttc tataatattt
     1381 atcaactact ttacttatag gttgtaattt aacttgatta ttttctttaa ttaaaaccat
     1441 ttcgtcacca cagattgatg ctcctacacc tacaaacatt tcaacaaaat cagaaccact
     1501 catgcttaaa aatg
//
